LOCUS       DACKAC010000001       101342 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.001, whole genome shotgun sequence.
ACCESSION   DACKAC010000001 DACKAC010000000
VERSION     DACKAC010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 101342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 101342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..101342
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.001"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            85..972
                     /locus_tag="I0B46_000001"
     CDS             85..972
                     /locus_tag="I0B46_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAR2082336.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTITSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1112..2038
                     /gene="cysK"
                     /locus_tag="I0B46_000002"
     CDS             1112..2038
                     /gene="cysK"
                     /locus_tag="I0B46_000002"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAR2082337.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2274..2720
                     /locus_tag="I0B46_000003"
     CDS             2274..2720
                     /locus_tag="I0B46_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAR2082338.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2993..4345
                     /locus_tag="I0B46_000004"
     CDS             2993..4345
                     /locus_tag="I0B46_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAR2082339.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4516..4803
                     /locus_tag="I0B46_000005"
     CDS             4516..4803
                     /locus_tag="I0B46_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2082340.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            5029..5472
                     /locus_tag="I0B46_000006"
     CDS             5029..5472
                     /locus_tag="I0B46_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAR2082341.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(5478..6662)
                     /locus_tag="I0B46_000007"
     CDS             complement(5478..6662)
                     /locus_tag="I0B46_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAR2082342.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            6930..7628
                     /locus_tag="I0B46_000008"
     CDS             6930..7628
                     /locus_tag="I0B46_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAR2082343.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            7635..8312
                     /gene="nth"
                     /locus_tag="I0B46_000009"
     CDS             7635..8312
                     /gene="nth"
                     /locus_tag="I0B46_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAR2082344.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(8438..8971)
                     /locus_tag="I0B46_000010"
     CDS             complement(8438..8971)
                     /locus_tag="I0B46_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2082345.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(9268..11649)
                     /locus_tag="I0B46_000011"
     CDS             complement(9268..11649)
                     /locus_tag="I0B46_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAR2082346.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(11639..12286)
                     /gene="recU"
                     /locus_tag="I0B46_000012"
     CDS             complement(11639..12286)
                     /gene="recU"
                     /locus_tag="I0B46_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAR2082347.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            12350..12889
                     /locus_tag="I0B46_000013"
     CDS             12350..12889
                     /locus_tag="I0B46_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAR2082348.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            12979..13398
                     /gene="gpsB"
                     /locus_tag="I0B46_000014"
     CDS             12979..13398
                     /gene="gpsB"
                     /locus_tag="I0B46_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAR2082349.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            13420..13787
                     /gene="rnpB"
                     /locus_tag="I0B46_000015"
     ncRNA           13420..13787
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="I0B46_000015"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            13996..15162
                     /locus_tag="I0B46_000016"
     CDS             13996..15162
                     /locus_tag="I0B46_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAR2082350.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            15208..16704
                     /locus_tag="I0B46_000017"
     CDS             15208..16704
                     /locus_tag="I0B46_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAR2082351.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            16785..17468
                     /locus_tag="I0B46_000018"
     CDS             16785..17468
                     /locus_tag="I0B46_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAR2082352.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            18113..19303
                     /locus_tag="I0B46_000019"
     CDS             18113..19303
                     /locus_tag="I0B46_000019"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAR2082353.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            19405..20358
                     /locus_tag="I0B46_000020"
     CDS             19405..20358
                     /locus_tag="I0B46_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAR2082354.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            20427..21776
                     /locus_tag="I0B46_000021"
     CDS             20427..21776
                     /locus_tag="I0B46_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAR2082355.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(21959..22282)
                     /locus_tag="I0B46_000022"
     CDS             complement(21959..22282)
                     /locus_tag="I0B46_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2082356.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            22411..23574
                     /locus_tag="I0B46_000023"
     CDS             22411..23574
                     /locus_tag="I0B46_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2082357.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            23644..24993
                     /locus_tag="I0B46_000024"
     CDS             23644..24993
                     /locus_tag="I0B46_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAR2082358.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(25038..25469)
                     /locus_tag="I0B46_000025"
     CDS             complement(25038..25469)
                     /locus_tag="I0B46_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAR2082359.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            25857..28499
                     /gene="alaS"
                     /locus_tag="I0B46_000026"
     CDS             25857..28499
                     /gene="alaS"
                     /locus_tag="I0B46_000026"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAR2082360.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            28699..29295
                     /locus_tag="I0B46_000027"
     CDS             28699..29295
                     /locus_tag="I0B46_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAR2082361.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            29417..30565
                     /locus_tag="I0B46_000028"
     CDS             29417..30565
                     /locus_tag="I0B46_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAR2082362.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(30603..31016)
                     /locus_tag="I0B46_000029"
     CDS             complement(30603..31016)
                     /locus_tag="I0B46_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAR2082363.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            31095..31526
                     /locus_tag="I0B46_000030"
     CDS             31095..31526
                     /locus_tag="I0B46_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAR2082364.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(31625..31858)
                     /locus_tag="I0B46_000031"
     CDS             complement(31625..31858)
                     /locus_tag="I0B46_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAR2082365.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            32041..33711
                     /locus_tag="I0B46_000032"
     CDS             32041..33711
                     /locus_tag="I0B46_000032"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAR2082366.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            33858..34244
                     /locus_tag="I0B46_000033"
     CDS             33858..34244
                     /locus_tag="I0B46_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2082367.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            34231..34929
                     /locus_tag="I0B46_000034"
     CDS             34231..34929
                     /locus_tag="I0B46_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAR2082368.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            34934..35398
                     /locus_tag="I0B46_000035"
     CDS             34934..35398
                     /locus_tag="I0B46_000035"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAR2082369.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            35395..36306
                     /locus_tag="I0B46_000036"
     CDS             35395..36306
                     /locus_tag="I0B46_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAR2082370.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            36541..37080
                     /gene="pyrR"
                     /locus_tag="I0B46_000037"
     CDS             36541..37080
                     /gene="pyrR"
                     /locus_tag="I0B46_000037"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAR2082371.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            37094..38377
                     /locus_tag="I0B46_000038"
     CDS             37094..38377
                     /locus_tag="I0B46_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAR2082372.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            38390..39316
                     /locus_tag="I0B46_000039"
     CDS             38390..39316
                     /locus_tag="I0B46_000039"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAR2082373.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            39335..40615
                     /locus_tag="I0B46_000040"
     CDS             39335..40615
                     /locus_tag="I0B46_000040"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAR2082374.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            40620..41696
                     /gene="carA"
                     /locus_tag="I0B46_000041"
     CDS             40620..41696
                     /gene="carA"
                     /locus_tag="I0B46_000041"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAR2082375.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            41701..44886
                     /gene="carB"
                     /locus_tag="I0B46_000042"
     CDS             41701..44886
                     /gene="carB"
                     /locus_tag="I0B46_000042"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAR2082376.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            44940..45716
                     /locus_tag="I0B46_000043"
     CDS             44940..45716
                     /locus_tag="I0B46_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAR2082377.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            45713..46645
                     /locus_tag="I0B46_000044"
     CDS             45713..46645
                     /locus_tag="I0B46_000044"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAR2082378.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            46642..47358
                     /gene="pyrF"
                     /locus_tag="I0B46_000045"
     CDS             46642..47358
                     /gene="pyrF"
                     /locus_tag="I0B46_000045"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAR2082379.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            47365..47994
                     /locus_tag="I0B46_000046"
     CDS             47365..47994
                     /locus_tag="I0B46_000046"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAR2082380.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            47982..48296
                     /locus_tag="I0B46_000047"
     CDS             47982..48296
                     /locus_tag="I0B46_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2082381.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            48334..48975
                     /locus_tag="I0B46_000048"
     CDS             48334..48975
                     /locus_tag="I0B46_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAR2082382.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            49006..49710
                     /locus_tag="I0B46_000049"
     CDS             49006..49710
                     /locus_tag="I0B46_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAR2082383.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            49707..50609
                     /locus_tag="I0B46_000050"
     CDS             49707..50609
                     /locus_tag="I0B46_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAR2082384.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(50767..52473)
                     /locus_tag="I0B46_000051"
     CDS             complement(50767..52473)
                     /locus_tag="I0B46_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAR2082385.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            52663..53286
                     /locus_tag="I0B46_000052"
     CDS             52663..53286
                     /locus_tag="I0B46_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2082386.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIQEEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            53736..54785
                     /locus_tag="I0B46_000053"
     CDS             53736..54785
                     /locus_tag="I0B46_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAR2082387.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            54782..55663
                     /locus_tag="I0B46_000054"
     CDS             54782..55663
                     /locus_tag="I0B46_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAR2082388.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            55666..56436
                     /locus_tag="I0B46_000055"
     CDS             55666..56436
                     /locus_tag="I0B46_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAR2082389.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            56715..57569
                     /gene="aroE"
                     /locus_tag="I0B46_000056"
     CDS             56715..57569
                     /gene="aroE"
                     /locus_tag="I0B46_000056"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAR2082390.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            57747..58910
                     /locus_tag="I0B46_000057"
     CDS             57747..58910
                     /locus_tag="I0B46_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAR2082391.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            59027..59437
                     /locus_tag="I0B46_000058"
     CDS             59027..59437
                     /locus_tag="I0B46_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAR2082392.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            59611..61839
                     /gene="topB"
                     /locus_tag="I0B46_000059"
     CDS             59611..61839
                     /gene="topB"
                     /locus_tag="I0B46_000059"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAR2082393.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            61977..62294
                     /locus_tag="I0B46_000060"
     CDS             61977..62294
                     /locus_tag="I0B46_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2082394.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            62454..62690
                     /locus_tag="I0B46_000061"
     CDS             62454..62690
                     /locus_tag="I0B46_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAR2082395.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            62687..63085
                     /locus_tag="I0B46_000062"
     CDS             62687..63085
                     /locus_tag="I0B46_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAR2082396.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(63752..64696)
                     /locus_tag="I0B46_000063"
     CDS             complement(63752..64696)
                     /locus_tag="I0B46_000063"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAR2082397.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(64992..65936)
                     /locus_tag="I0B46_000064"
     CDS             complement(64992..65936)
                     /locus_tag="I0B46_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAR2082398.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            66157..67371
                     /locus_tag="I0B46_000065"
     CDS             66157..67371
                     /locus_tag="I0B46_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAR2082399.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASIYGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            67393..69408
                     /locus_tag="I0B46_000066"
     CDS             67393..69408
                     /locus_tag="I0B46_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase"
                     /protein_id="HAR2082400.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEETFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(69491..70864)
                     /locus_tag="I0B46_000067"
     CDS             complement(69491..70864)
                     /locus_tag="I0B46_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAR2082401.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(70890..71174)
                     /locus_tag="I0B46_000068"
     CDS             complement(70890..71174)
                     /locus_tag="I0B46_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAR2082402.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(71176..73191)
                     /locus_tag="I0B46_000069"
     CDS             complement(71176..73191)
                     /locus_tag="I0B46_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAR2082403.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(73370..73675)
                     /locus_tag="I0B46_000070"
     CDS             complement(73370..73675)
                     /locus_tag="I0B46_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAR2082404.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(73764..74795)
                     /locus_tag="I0B46_000071"
     CDS             complement(73764..74795)
                     /locus_tag="I0B46_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAR2082405.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            75002..75877
                     /locus_tag="I0B46_000072"
     CDS             75002..75877
                     /locus_tag="I0B46_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAR2082406.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(76029..76871)
                     /locus_tag="I0B46_000073"
     CDS             complement(76029..76871)
                     /locus_tag="I0B46_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAR2082407.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(76963..78840)
                     /locus_tag="I0B46_000074"
     CDS             complement(76963..78840)
                     /locus_tag="I0B46_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAR2082408.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVCNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(78837..80258)
                     /locus_tag="I0B46_000075"
     CDS             complement(78837..80258)
                     /locus_tag="I0B46_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAR2082409.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(80261..83650)
                     /locus_tag="I0B46_000076"
     CDS             complement(80261..83650)
                     /locus_tag="I0B46_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAR2082410.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            83907..85298
                     /locus_tag="I0B46_000077"
     CDS             83907..85298
                     /locus_tag="I0B46_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAR2082411.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(85371..86018)
                     /locus_tag="I0B46_000078"
     CDS             complement(85371..86018)
                     /locus_tag="I0B46_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAR2082412.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(86026..86712)
                     /locus_tag="I0B46_000079"
     CDS             complement(86026..86712)
                     /locus_tag="I0B46_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAR2082413.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(86718..87791)
                     /locus_tag="I0B46_000080"
     CDS             complement(86718..87791)
                     /locus_tag="I0B46_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAR2082414.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            88013..88687
                     /locus_tag="I0B46_000081"
     CDS             88013..88687
                     /locus_tag="I0B46_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAR2082415.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            88692..90032
                     /locus_tag="I0B46_000082"
     CDS             88692..90032
                     /locus_tag="I0B46_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAR2082416.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(90181..91167)
                     /locus_tag="I0B46_000083"
     CDS             complement(90181..91167)
                     /locus_tag="I0B46_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAR2082417.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            91891..92922
                     /locus_tag="I0B46_000084"
     CDS             91891..92922
                     /locus_tag="I0B46_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAR2082418.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            93031..94563
                     /locus_tag="I0B46_000085"
     CDS             93031..94563
                     /locus_tag="I0B46_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAR2082419.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            94702..95514
                     /locus_tag="I0B46_000086"
     CDS             94702..95514
                     /locus_tag="I0B46_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAR2082420.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            95504..95989
                     /locus_tag="I0B46_000087"
     CDS             95504..95989
                     /locus_tag="I0B46_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAR2082421.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            96012..96557
                     /locus_tag="I0B46_000088"
     CDS             96012..96557
                     /locus_tag="I0B46_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAR2082422.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(96630..98273)
                     /locus_tag="I0B46_000089"
     CDS             complement(96630..98273)
                     /locus_tag="I0B46_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAR2082423.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(98402..100630)
                     /locus_tag="I0B46_000090"
     CDS             complement(98402..100630)
                     /locus_tag="I0B46_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAR2082424.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            complement(100663..101142)
                     /locus_tag="I0B46_000091"
     CDS             complement(100663..101142)
                     /locus_tag="I0B46_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2082425.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
BASE COUNT        32052 a        18131 c        20467 g        30692 t
ORIGIN      
        1 aattaaaagt ataatttaat taattaagtt tgttttaaga agagaacttt ttattaagtt
       61 tttttatttt gaggaggaat ttgaatgcaa gagctgaaga cccgcagtgc ccggcaccat
      121 agacctaaaa gaaaaatggt gatcaataaa aaagcgattg ctcctttaat tggaacgagt
      181 cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc agatgaacaa
      241 ggatctcaaa cagtcgctga tcctcaagcg tttatcgatc aaatcggctg gtctgcgtca
      301 gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc gttgttggaa
      361 agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt cggtgtgaaa
      421 ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaaa cggacaatgg
      481 gtaacgatga atgagccatt ccgaaaatat tcttcttatt gggaatcctt ccaagaccat
      541 gcgaatgttc ttcgttcaac cagtttttcc acgggcgatt atcattatgc tggcgtgtgg
      601 aaaagcaata caacaagttt ttatgatgca acagcgtact tgacaggtcg ttatgctacc
      661 gatccaaatt atggggccaa attgaactca ttgattaata cttataattt aacccgtttt
      721 gatacgccat caacaggttc ggctacaaca atcacaagta caacaacaac gactactacg
      781 acgacttcaa cgtcatcatc ttcaacaaca gatgtaaata caaataatgg tcagacatat
      841 acagtcgtgt caggtgatac gctatgggat attgcacaaa aatttggcat gtctgttgat
      901 gatctaatgg cgaaaaatgg catgtctatg tccagttatt ctttattagt tggtcaagaa
      961 ctaaacgttt gatttagata aaaaggcaaa gaaattacca tatatggtat atctttgtct
     1021 tttttttgct agtcaaagac gaatcactcg ccataaatga acagattgta ttatgataag
     1081 taagaagagc aaagacaagg aggaattgaa gatgacagct atctataact cagtcaccga
     1141 attgattggc aaaacgccaa tcgtcaaatt aaataaaatc gtaccagaag attcagcaga
     1201 tgtttttgta aagcttgagt tttttaatcc aggaggaagt gtaaaagatc gtattgcttt
     1261 aagtatgata gaaaaagcag aacacgatgg tctactgaaa ccaggtgata ctatcattga
     1321 acctacttct ggaaatacag gaatcggatt gtcgatggta ggagttgcaa aaggatataa
     1381 agtaatcatc gtgatgcctg agacgatgag tatcgaaaga cgtttattga tgaaagggta
     1441 cggcgcagag ttgattttga ctcctggtgc ggatggtatt tctggatcaa tcagagaagc
     1501 agaacgtttg gcaaaagaaa acggctactt tttaccatta caatttgaaa atgaagcaaa
     1561 tcctcttgtg catgagaaga cgactggtcc tgagatacat caagcatttg gagtaaatgg
     1621 cttggacgcg tttgttgcag gtatcggtac aggaggaacg atcactggcg ctggccgaga
     1681 attaaaacgt gtgtatccga aaatcgaatt aatcggggta gaaccagcag aatctgctat
     1741 tttagaaggg aaagaagcgg gacctcacaa aatccaagga atcggtacag gttttgttcc
     1801 taaaacactg gatacttctg tgtatgataa agtcctttct attagtggag acgaagcgat
     1861 ggaaacagct agagaagttg ggcgaaaaga agggatttta gtaggcatct catctggcgc
     1921 tgcaattgct gccgcgctaa aagtcgcaaa agaattagga aaaggcaaga aggtattagc
     1981 tgtggttccc gacaatggtg aacgttattt atctacagcc ctttaccaag agatataagg
     2041 cacttggttt ttctccagat aggtttatgt aaaaaacttt taaagatgaa aagaacacac
     2101 aaaaatttat gatgagaaaa attatttgaa tagagacgta gaatagtgaa cgcgactgtt
     2161 ttacgtcttt tttcattaaa atacaataaa attgaatttt cttttcatgt taagtggatt
     2221 ttaaaatgta aaaatagtat gataagacga gaataacaac gtggggtgtt tacatggaaa
     2281 ataagttagt cgaagaggtt atcgaaaagt taaaaaaaga gaacattcga atcacacctc
     2341 aacggtacgc ggttttagaa tatttgattg aacatcattc ccatccaacg gctgatgaga
     2401 tttataaagc acttgaacat cggtttccaa atatgagtgt tgcgactgtt tacaataacc
     2461 ttcgcttatt tacttcgatt ggatttgttc aagaaatgaa gtacggcgat gcttctagcc
     2521 gatttgattt cagttcaaag cgtcattatc atgtaatttg tgaaaactgc ggtaagattg
     2581 ttgattttca ttacccaggg cttgaagata tagaaatggc tgcaagcaga ctgactgatt
     2641 ttgagattaa tgaacatcgg ttggaattgt atggactctg tccggattgt aaagagtcag
     2701 caaaaaaggc gcaaatgtaa aaacagtttc tacaagtgtt atacctaaaa aaagctagct
     2761 gtgtaaaaaa aacgctagct tttttatttt tttgtctttt attcacaatc ttagatataa
     2821 aagtgaatat tattaaaata agaatcttgt agatattggg ttcttatgct tgaaaaggaa
     2881 tgaaactaca aacaaacctt tgtgaattca caagcattaa actgttgtat ccgttttatt
     2941 tctgttatag tagttttgtc gaaggacaaa atgttaatcg gagggaccat aaatgaaagt
     3001 agtagtaatt ggctgtactc atgcaggtac tgcagctgta aaaagtattt taaaaaatca
     3061 tccagaagca gaagtgacag tatatgaaag aaatgacaat atttctttct tatcttgcgg
     3121 tatcgcactt tatgtcggcg gagtagtaaa ggacccagca ggcttgttct actcaaatcc
     3181 tgaagaatta tcttcattag gagcaacagt taaaatggaa cacgatgtaa cagacgtcga
     3241 tacagaaaat aaaacagtaa cggcaaaaga tctgaaaaca ggagaaacag aaacggtttc
     3301 ttatgataaa ttagtgatga caactggttc atggcctatt atcccaccga ttagtggaat
     3361 cgatgcgaaa aatgtactcc tatgcaaaaa ctacaatcaa gcaaatgaaa ttattgcaca
     3421 agcgaaagaa gcaaaacgtg tagtaattgt tggtggtgga tatatcggta ttgaattagt
     3481 agaagctttt gtcgaatctg gaaaacaagt gacattgatt gatggattag atcgcatctt
     3541 gaataaatat ctagataaac cattcacaga tatcttagaa aaagaattga cagaccgcgg
     3601 tgttactctt gcgctaggcg aaaatgttac tgaatttatc acagatgaag aaggaaaagt
     3661 gaaacaagta gctacaccaa cagatacatt tgatacagat atggtcattt tatgtgtggg
     3721 cttccgtcca aatacaaaac tgatcgaagg aaaagtagat acactgccaa acggggcaat
     3781 caaagtgaat gaatacatgc agacaagtaa tccagacatt tttgcagctg gagactctgc
     3841 agtagtcaac tacaatccaa gtggcacgca aaactatatt cctttggcaa caaatgccgt
     3901 tcgtcaaggt ttgcttgtgg gaaataactt gacagatcat aaaatggcgt atcgtggtac
     3961 tcaaggaact tcaggacttt acctatttgg ctggacgatc ggttcgacag gtgtgaccaa
     4021 agaaagcgca actttgaacg atctagaagt ccaagcgact gtatttgaag ataattatcg
     4081 tccagagttc atgccaacta ctgaaaaagt aatgatggag ctagtctatg aaaaaggaac
     4141 gaatcgtatc gtaggtgctc agttcatgtc caaatacgac attacacaat cagcaaatac
     4201 catgtcttta gctgttcaaa ataaaatgac tgtagaagat ctcgcacttt cagatttctt
     4261 cttccaacca cattttgacc gcccatggaa ttacttgaac ctacttgcac aagcagcgct
     4321 aggcgaaatc gaaactgcac aataaagaaa aagggttgtg acacaatttt gtcacgaccc
     4381 tctgtctttt tttgtgaaaa gtacaattag ttgtctttct tcgaattttt tgtcacaata
     4441 agcagcaaag ataaagctgt tacataaaag aacaagcagc tttatttgat tttgaaaaga
     4501 aaggtgagaa agaagatgaa taaagaatat tttgtccaac aagatgcatt tttagaagat
     4561 tataaaagtg cttcgaagaa attaacgatc aatattgatg agttggcaga tcaagtactc
     4621 tctcagttga atgaaacgca agaggaatat tacacgatgc cagcaaatga cgccaaagat
     4681 aatgtttctc atcgttttcc tttcagtaga cgcatctact cagatgaaaa tgacggcaca
     4741 ttgaatacag agtacacata tgaaggtcag ccatatgtgc tagaaactga tgatgatctt
     4801 taaagacagt ttttgaaaag gccgctcatc cttaagtgat gacaaaaaat catcacaatt
     4861 accgatgact gtctctttac tatcttttaa tcagaaaaag gggttatgac aagctttgtc
     4921 aacccctttt tttcttggtt tcagattggg ttttcctaca aaatattttt atctttgttg
     4981 aataggaaac aaaaaaacaa tataatgaaa gaaaaagaag gtgtagaaat ggataaaaga
     5041 caactgctaa tagaagaata tctggattta gctaatgaga ttattcatcg taataaacca
     5101 gaaatggaaa aagaatttgc aggatttact ctaaatgaac tagaagtgat tgaacatatc
     5161 gggaaaatcc ctgatcctaa cgtgacaaaa ttagcgaatg ctagttacat gactagagga
     5221 gccatcagta agctaacaaa aaaactgata gctagaggga tcataaacac ttatcaaagc
     5281 gaagagaata aaaaagaaat ctattttcaa ttgacgccaa tcggacaaaa aataaggaat
     5341 agacacgagc aactccatca aaaatgggta cagcgagatg ctactgtatt tgaaaatatg
     5401 tcaaaagaag aatttgacac agtatttcgt tttatcggtc gatacagaga atttatacga
     5461 agtagcaaat gaagctacta ttttttattg tagtgataat aggcaaaaat cataagcaaa
     5521 aatccgaata ccgtcggaag aaatggtgct gcagcataaa gctgtcctgc gattagcggg
     5581 ccgatgacgc gcgatagaga ttgtatactt tgggtaccgc ccattacaag accttgctgg
     5641 ttatctgaga cagaattgga tagttggcca ttgaatacag gagtaaaaat agaatcaccg
     5701 aaaccataac agataatccc aaataaaaac aaaatcggca gtagaagaat tccagataat
     5761 gtgatgaata gataggcgag gatttcactg aacatcccta ttcttgtgat ctgctgttct
     5821 gaaaattttt tgagcaatcg aggcataatg aacaattgcg agacagtatc caagattcca
     5881 atcaaagaga agcttaaacc aaccaaccca gccttccact gaaaggcgtc aatcgaaaat
     5941 tgagaaaaga tagattgaag agagccagct gccaaccaaa cagcaaaacc tgctgtcaat
     6001 aaaggaataa cggatgaaat cttgaataat tgttgtaatt ggtgaaaagg tcttacatgg
     6061 gagaaactta aatctactga acgttttttt attggaaggc tttctagcat aaatgtgtag
     6121 ccataaacgg catttaagaa agtaaagagt gctccaataa atataggaac agaattcccc
     6181 aactcagcta atagaccccc tataatagga cccagcgttg ttcctatacc gactaaagca
     6241 cccatccatc caaaaacttt ggttctttca ttaggttccg tgatgtcggc aaagtaagca
     6301 tataaggtgg agatttctcc tgccgtcaat ccttcaatga tacgaccaag gaacaacatc
     6361 catatcgaat ttcctaaacc aaaaagcaaa tatccgatac ttgatcctaa taaactgata
     6421 ataaggatcg gatgacgccc aaaatgatca cttaagctac cgagaatggg ggcagataag
     6481 aaggcagcta gagcataaac agacatgagc aaagtgatat agaaagcttg ctgatgtatg
     6541 gagtgagaat aaggcagcgt taaaaacgga agtacaggac tgacgatagt aaatcctaat
     6601 ccagtgaata agacagagac gtatccaaaa actttagaat gtttatttgt gaaactgtac
     6661 atgtaaaaac tcctttatgc aataatgtta acttggaaac aaaaatagtg tatagtaatt
     6721 ttgtttcctt gtcaacattc taaggaacag cgaaatctac ttaatttatt tattccgaaa
     6781 agtgaagata cgcatcaaaa acatgaacaa acaaaaatca agtagacgac attgtaaatc
     6841 tgaccgatca gtgaagtaaa tagaaaagaa acctgttctt gcttcccatg ttttttttac
     6901 gtatactgtg attgagcgga ggagaaatca tgttgaattt agaaaattac ttaagcgctg
     6961 gacaaacagt catatctaat cttttactta aaaattatca taggatcggt ctacaatctg
     7021 aagaattttt atttgttcta cagttgcata tggcacagct tgaaggagat actttccctg
     7081 atttgcagat gattgctatg gatatgggaa tcaaacaaga tcagattttt caaatactgg
     7141 atcgtctcgt aacaaatgga tttatcaaga tagaaacgac gattgtcaac ggtaaaaaag
     7201 cagatcgata taatctttac cccatctatg atctattagg tgagtattta aaaacacaag
     7261 aaaaaaaata cgaagaaaaa acacaagaaa aagaaatcca gtcagtgtat caattatttg
     7321 aacaagaatt tggacgcccg ctttcttcga tcgagttcca gcggatcgga caatggctag
     7381 aagaagacca ttatcagccg gaaattttga agctagcact gcgggaagcc gtattaaatc
     7441 aggcttatag ttttaattac gttgaccgca ttttgctatc gtgggaaaga aaaaatctac
     7501 gtacaaaaca acaagtggaa gaagaccaaa aacgaagaaa acagcagctt cttcaaagag
     7561 aagtagaaca gtcagctcaa caagaagaat tgccgaaagt ctcactgaaa aattggttgg
     7621 aggagtagcg gaatatgtta agcaaacaaa aaacaatgga agctctagaa acgatgtatg
     7681 gaatgtttcc cgaagcacat ggagaactca agcacaataa tccatttgag ttattgattg
     7741 cagtgatctt aagtgcccag gcgacagatg tatcagtgaa taaagcaacg ccagatttgt
     7801 ttgcctcttt tccgacaccc gatgcgttag cagaagcttc aatcgatgaa atcattctta
     7861 aaataaaaac gattggtttg tatcgaaata aagcaaaaaa catcaaagcg tgtgcacagc
     7921 agctgattga acgtttcgat gggcaagtgc caacgtcaag agaagaattg atgtctcttc
     7981 ctggtgtagg cagaaagaca gcaaatgtcg tattggggga tgcattcggg ataccagcaa
     8041 tcgcagtaga tacccacgtg gaacgtgtct caaaacgcct gaggatctgc aaactagatg
     8101 ctacggtcat ggaagtagaa gaaacactga tgcggaaagt accacaagaa ttatgggtaa
     8161 agacacatca tacactgatt ttttttggta gatatcattg cacggcaaga aatcccaagt
     8221 gcgaggtatg tcctttgcta tcaatatgtc aagatgggaa aaatcggatg cgtctaaaag
     8281 aaaaggcgct aaaaaagaaa ccacgcctgt agacgtggtt tctttaattg caaataaatg
     8341 tatgaattat cttgttatta tggagaaaag ctgtttcgtt tatctttttt gcatgaaaaa
     8401 gtaaatgtac atactcagta gctaggatgg taacttatta caatttattg cgataaaaat
     8461 aaaagttgtt atttttattg ggaaaatacc cgatgtagaa aataatgcct tccaatatac
     8521 ctataaaata agtccagtat tccaaaggat tatataaata agtgacgtat ctatctaaag
     8581 gtgtaataaa aatgataagt gataataaaa aaataccatt tataactaaa cataaactaa
     8641 tggatttggg cggtttcagg ctcatcagaa aaatatagat tctggtgaac aacaaccata
     8701 aaaaagtgag aatcattgca tgagagcttc tttccataaa ttttccttta agagtagtgc
     8761 ttaaaagtac agaaaaaata ataaatgcaa ggataggagc aattaaagta aatttattta
     8821 ataataaaca aaatttttca gcttttttat tcttggaata acgaggtatt gttttcgata
     8881 aaaaaagatg gtgcagacaa attatgataa ttaagcatac tatcagataa ctcaaaatga
     8941 agccttcttt caaaaaatta ttcaaaacca tatattgtat tttttatttg ataataacaa
     9001 tgggattatt aagaagtggc actaaataaa agactagtgt gtatttagcc gatttaatta
     9061 taaacaagta aatggataaa gtatatgtaa tgataaaaga aaaatggatt ttctaatttt
     9121 taagctaaat gttagatcac acatgtatat gctggatagt cttataaaaa atcatgtaat
     9181 acagcttggt acatgatttt tatttgtagc tgttttttac atagacacaa aaaagaaacc
     9241 acgcttgtag acgtggtttc ttttttacta tctgtttcca gaagctgagg tgctgctgga
     9301 tggtgtatgg tcatttgcag catccgctcc gctagttgct ggctcactgg aagaagcagg
     9361 aggagcagat gaagacggtg tcgagctttc ttcggatgaa gaagaaggaa ccgttgttga
     9421 agacgctggc tcgcttgttt ctgctgtcgt gctttctttt gaggactcag cagttgattc
     9481 gctttgacta gtagagctag agctttgagt cgttgtctct gttgttgagc ttccaggagt
     9541 ctggacatag ctggaagatg gaatagttgt tgatggtgta attgcattcg atctagctgt
     9601 atattgatct ttgtagtaca gttcaccgcc cacacgaatt aatccgcttg gcatctccca
     9661 gtctgtattt gtcacgttag ctgaaacata ctgcataagc tcgcgataaa catcagaggc
     9721 tacgtgtgac gattctgaag tgacaggtgt cattttttta ttgtaacctg tccacacgga
     9781 aatcgaataa tttggcgtat agcccgcaaa taggatatct ggatacaccc cagaagaaat
     9841 accaagtttt gcatactcgt catctgtgta attggatgtt ccggtcttac cggcttgata
     9901 taacccagcg atttgcgcat tcgtaccagt tccttcagtg atcgtatctt ttaaaatgtc
     9961 tgtgatcata taagctgttt ctggagacat ggctgttttt ccatctggct cataagtttc
    10021 ttctgttccg tcttggaaga cgattttatt aacatattga ggtttgtaat aagtccctcc
    10081 attggcgaaa gccgcatagg ctgctgccat ttttaaggaa gaagctccgt attttgtccc
    10141 atcttgttct tccgtattac tagagatcgc attcgattga tggatcgtac tgtattcgat
    10201 cccaagattt tttaagaaag aagccacttt atctgaaccg acttcattga ataatttgac
    10261 tgctggaaca ttacgggata agtaaagtgc ttggcgtaaa gtaatggtgc ccatatattg
    10321 attatcccag tttcctacag gagtcgaagt cccttcatag ttatacggtg cgtcagtaat
    10381 agtttttcca gtagaatatt tcaaatattc aaaagctggt ccgtaatctg tgacaggttt
    10441 catcgtagaa ccaaaatcac gggaagtatt cactgctaag ttgttgccta aagttacgtc
    10501 ttctgcgatg tgacgtccgc caatctgggc ttttactttc ccggtattgg tgtcgataag
    10561 ggtggaggca acttgcatct cgtcgtctgg gtaagagaca tactgatccg tgttgacgat
    10621 atcatatagt ttcttttggg cgtccagatc caaattcgta taaatctcta aaccgtctgt
    10681 gtagacattc ttatctgttt tttcttgtac ttcattgatg acttctttga cataattatc
    10741 gacgatctta gtattgtcat cggattgagt tagttcttgc agaccatccg ttaccggtac
    10801 attaacagct tgatcatatt ctgtttgaga gattttttcg ttttgaagca tcgtatacaa
    10861 aacagtatct cgtcttttct tcgcctgatc tggataaaca taaggatcat aggcggaagg
    10921 ggcttgaggc atcccagcaa gcaatgctgt ttgcggtaaa gaaagttctg ataatttctt
    10981 accaaagtac atctcagaag ctgtctccat cccgtacaag ccgtttgaca tatacacttt
    11041 atttacgtag taggtcaaga tctcttgttt cgattttttt tgttccaatc ttacagccat
    11101 ccacgcttct tgggccttgc gtttcagagt ctgatcctcg gcactggtgg agaagaagga
    11161 aagtttgatc aattgctgtg tcaaagtact tccgccttgc agtccgccag aggtaaagtt
    11221 agagagggca gaaccgatga tccgaatagg gtcaaccccg atgtgtttat aaaaacggcg
    11281 atcttctacc gacacgatcg catcttctaa tgttttcggt aattcattcg cagaaatttt
    11341 ttcacgcttt tctgcgccta aatcttcgaa tagttcgcca tcttgtgtat atagtttgga
    11401 agatacggta gcgtctaatt ttttatccgt caattcgggc gcatcttttg cgtaatacca
    11461 gaaaagtcct acacctgcta aaaagaggat acagaaaaat gagagtattc ctaaaaggat
    11521 cttgataaat aatccttttt tatgtgttcc agaggacttt ccagaacctt tgccagaatt
    11581 cttcttcaca ctctttttag gagtaggctg tttctgtcgt ctagagcttc tagtttgttc
    11641 atttgccatt cttgctttct ccttcaatag ataatgttga taaatagaag tcaaccgcgt
    11701 ccaaataagg aatccgcgga gaaataccag ttttgatctc tattcctagt tcttcgatca
    11761 aagataaagg tagtgatttg cgtccttttt ccttttgggc gtcccagtaa gcaatcaaat
    11821 gaacactatt caaataaaaa cagcgcttca aagacgaaaa ccataacaag acaaagcaga
    11881 ccccattttg ttcgaggcat gctttcatat gttcgatttg atgggcatgg aagtttttaa
    11941 agggaaaaga tgttttgttc ttggtttctt ttgcttcaaa gtccaagtaa aagcctcgat
    12001 agacgccatt atagtctgta gttgaggctt gggtaaagta ggcttccttg atcacagcag
    12061 cacttctctt aggatagtct actttcacga tttggatagg ggtaggcttt ttatggataa
    12121 ccgccaaccc gcgggaaaga taatatttat tactttcatt gattgcttct tcaaagcgca
    12181 tcccccgatt gccgaattca gtgatctgat ttcttttttc ttgaattttt tcgacgggag
    12241 aggcattctt ataataaggt tggccatttg gatagcgcaa taccatcttt aatcacactc
    12301 ctaggccaca ttatagcaaa aaagcggata gaaagaaaag gaatctgaga tggataagtc
    12361 aaaagtaatc tatgtgtctg ggtatcgaag tttcgaactc ggtgttttta aagaaaatga
    12421 tcctaaaatc caagttgtta aaaatgtttt aaaaaaagaa ctccatcagt tagcagaaga
    12481 agggctggaa tgggtacttg tcagtgggaa tttgggcgtc gaactatggg ctgtgcaaat
    12541 agtagcggaa ttgaaagaag aatatccgga actatctctt ggtattttgt atcctttttc
    12601 tgattttggt aataactgga atgaagggaa ccaagaaaaa aaacaattag cagaacaatt
    12661 agcagattat gtcgaagctg ttagccatca accctaccaa tcacctagtc agctaaagaa
    12721 tcacactcgt tttatacttg agcatacagt gggctgtctg ctgatctatg atgaagaatt
    12781 ccctggaaaa acgaagtatt tttttgaaga tgcacagaat taccaagcag atcatcccta
    12841 cgaaattcgg ctgatcagta tggacgatct tcaaaatttt agtgaataat cgatattgtg
    12901 tttgatttat caactttttt cctatacaat gaaaaaggta gaaatgttat tacgtaaatg
    12961 aaaactagag gtgtaaatat ggcgaatttg gtttatagtc ctaaagacat tttacaacaa
    13021 gaattcaaaa caaaaatgcg cggctatgat cccgttgaag ttgatgaatt cttagacaat
    13081 atcatcaaag attatgaaac ttacagcaaa gaactccttg ctttacaaga agaaaacgat
    13141 cgattgagtg caaaagtagc tcaattatct aaaactcaag gagcagctca aacacgcgtg
    13201 caacaaacag aggcaccaaa gagcgcagca gtgaccaatt ttgatatctt aaaacgcttg
    13261 tcaaatcttg aacgtgaagt atttggtaaa aagcttgatc aacaagcaag tgcagtcaaa
    13321 ccagcacaac ctaatccaaa taactacaca aatgcagaca caagcttaga tgataatgaa
    13381 aaaacacgtc aattttaatt tttgattaat gaatttgcgg ttttcgggta atcgcggtct
    13441 gacttgatca ggctgaggaa agtccatgct cgcacaggct gtgatgcctg tagtgttcgt
    13501 gcttagcgaa accataagct aaggtactct ttggagtaac ggcaggaaaa caagctaagg
    13561 cttcggctat gcttaagtat ccttgaaagt gccacagtga cgaagcggct ggggaaaccc
    13621 atccggtgga acgcggtaaa cccctcgagc gagcaaccca aacaatggta ggggcgcttt
    13681 ttgtctggaa gaagaacagg acaaaaaggc agtataactg cagatagatg gttaccgctt
    13741 tgttttttct ccctggaaaa gcaaagtaca gaacatggct tatagaaaac cgcaaacatc
    13801 agggaattag gtcgtgacaa aagtaatgcg ttataagaaa caagaaaaac atttgaaagc
    13861 tatttttcaa tacttcagat gaatcgttct tattacaaaa caaacttttg tatcaccggt
    13921 tattcggaat ttgagtaaaa gtgtggggta ggactgatgt cccacctcct ttttttataa
    13981 ataaggagat tgtgtatgga aaaaaacagg aagttcaacc tcgtcgcaac tgcggcaagc
    14041 gggctagaag cactggttgg taaagaatta agagatcttg gaatcgaatg ccaagttgag
    14101 aacggacgtg cacgttttga aggaacaatg gaaacgatcg ctacagctaa tttatggtta
    14161 cgtacagcag atcgagtaaa aatcgtggtt ggggaattcg atgcgtatac gtttgatgaa
    14221 ttgtttgaaa acgtaaaagc attgccttgg gaagattttc ttccactaga tgcagctttt
    14281 cctgtagctg gaaaatcgat caagtcgaaa ctatatagca cacctgattg ccaagcgatc
    14341 acaaaaaaag caatcgttga acgtctccgt acctattatc accgtccagc ttctgtacca
    14401 ctgacggaaa ctggcgcaca ttttcagttg gaagtagctt tgctgaaaga ccatgtgatg
    14461 gtaactctgg atacgacagg accaagttta ttcaaacgtg gttatcgctt agaaaaaggt
    14521 ggagcaccat tgaaagaaaa catggcagca gcacttatta tgttgacaaa ttggcgaaaa
    14581 gaccgtccat tttatgatcc tgtatgtggt tcaggtacgc tttgtattga agcagcctta
    14641 ataggccata atatcgcacc aggattcaat cgtgagtttg cttgcgaatc ttgggattgg
    14701 ttcagtcaag aaatcatgga aaatgttcga tcagcagccg aagaaaaagc agattatgat
    14761 attgaattag atattactgg ttcagatatc aatggacgga tgattgaaat tgccaaagcc
    14821 aatgcagaag aaatcggatt aggctcttcg attaccttta agcaacaagc agtcaaagac
    14881 ttcaaaacgg ataaagaata cggggtcatc gtggctaatc cgccttacgg agaacgtcta
    14941 ggagaagaag aaactgttcg gaaattatat aaagaaatgg gcgatgtatt ccgtccatta
    15001 aaaacatgga gtaaatatat cttgactagc gacttagcgt ttgaggaatt ttacggacaa
    15061 aaagcaacca aaaaaagaaa attgtacaat ggtgcattgc gtacggatct attccagtat
    15121 tggggaactc gtccgcctag aaaaaaagag aaagacgaat aatcttacta attgattcat
    15181 tcaattggat gtttggagga atagggaatg aaagaaaaag agtttttaca agaagtaaaa
    15241 gaaatcaatc ttctacaaca agctttagga atcctcgatt gggatacaca aacaggtatg
    15301 cctgaaaaag ctagtgcata ccgaagtgaa gtagacagct atctttacag catttatttt
    15361 tcaaaaaaaa tcggtccaaa aatcaaagaa gccattcgct atttttctga acatccggaa
    15421 gaattgtcgg aagttggaac tgccgttttt gccaaagtaa aagaagagta tgatttggaa
    15481 catcgcgtac cggaagatct tatgagaaaa tatacagctg ccgtttcttc agcacacgga
    15541 caatggcaaa aagctagaga aacacaacaa ttttctgatt ttcaacacgc attgaatcaa
    15601 aatattgaat taacaaaaca gttgatacct tactggagaa aagatgaaaa gacgaattat
    15661 gatgttttgc tgaatcaata cgaacccggg atgactgtcg aaatccttga taaggtattt
    15721 gatcagctta aagaaggaat tctttcgatc cgccgtacgc tccaagaaaa agggactgaa
    15781 cctgaaacag actttttaaa tcggagaatg acgaaaaaac aacagaagag atttgtagtg
    15841 aaagtaatcg aacaacttgg ttatgatttt agtcgtggtc gtttagatga cactgtccac
    15901 ccatttatga ctggaattaa tccgaatgat gcacgaatca cgacacgatg gaacgaaaca
    15961 gatttcaaaa tggctgtttt tggagtgatc cacgaggctg gtcatggtat gtatgaacaa
    16021 gacatcgatg ataaatatgc gtatacaccg gtttatgaag gtacatcaat gggtatccat
    16081 gaatcacaat cgttgtttaa cgagatcatt attggaagta gtcgcagctt ctggaaaaag
    16141 caatatcctt tctttcaaga atgtgcagaa ggaacattcg atgatattcc ttttgacaaa
    16201 ttttatcgtt cattgaacca tacaaaatca agtttggttc gtatcgaggc agacagtttg
    16261 acttaccctc tccatatcat cattcgttat gaaatcgaaa aaatgatttt caatgaaggg
    16321 gtagatgttg ctgaactacc tgaaatatgg aatcaaaaat atgaagagta tctgggaata
    16381 cgaccaacaa atgatgtgga aggcatccta caagacgttc attggtcagg tgcaagcttt
    16441 ggttattttc cctcttatgc attagggtac atgtatgcgg cacaattact tcattctttg
    16501 cagcaagaaa tagatgtgga aaatgttcta gcgtcagacg attattctcc aatcaaaaac
    16561 tggatgtcag agcatatcca tcaatatggt gcatctagaa aaccaaatca attaatcatg
    16621 gatgcaacac atgaaccgct taatccgcaa tacctgatcg actatatgaa agacctgtat
    16681 tttaatgtat atcaggtaaa ataaaataca atttacaaag atagctgaaa ttcatctaat
    16741 ctatagatgt tttcagctat ttacttaaga aagaagtgga acgaatggat aaattacaag
    16801 tgatcgctga ttatagtatg gaccaattag cgcatgatca aacaggacat gggagcgatc
    16861 ataccaaaag agttgtcaag ttagctgaaa aaattttgga gacggaaccg caggcagacc
    16921 gctttgtgac gcttgcagca gcttacctac atgatacgat tgatgataaa gtcgttaaaa
    16981 atgagaacga ggcaaagcaa cagcttcgtg tattccttcg ctctttgcca atagtagagg
    17041 aacagatctc gatgattttt tccattatcg aaaatatgtc cttttctaaa aatctctctg
    17101 aagcggccga actatctctg gaaggtaaga tcgttcaaga cgctgatcgt atcgaagcat
    17161 taggagcaat cggcattttg cgtactgctt atttcggcgg tggacatggg cacccgattt
    17221 ttgatcctga actgtatccg caaacattca aggacaaaga aaattaccga aaaggcacga
    17281 cagtgatcaa tcatttttat gaaaaactgt tcttgctgtc tgataaaatg aatactgatt
    17341 acggatatga agaagccaaa cgaagagaaa ccttcatgcg tgaattctta gaagaatttt
    17401 ttctggaatg gtctattgac gatgaaaatt ttactccaga caattggatc aaaaataaca
    17461 aaaaatgatg gaggttgtga cagaagtggt cagctccaag atatttcgga tcattttccg
    17521 taaaagttac ttctggaaca ctgtttacca agaggtcgtg agcctgccgt tcagctccga
    17581 gtcacaagga acaatttcaa aaaatagctt ctcgtgtttg atgaatattg taacttgtga
    17641 ttcggaattg gcaggagaac accgtttatt cggaagaata ggactgtgac aagacttttg
    17701 tcacagtcct tttttcgaat tcaacaagta aaacttttaa gaaatattca gagagtaaaa
    17761 ttaaaaattg ctgataaacg aagatggaaa ttttatgtat ttttattggt ctatgcaata
    17821 aataaattaa atattcagaa tacaattgat tttaacgaaa gggcttctat caatggttgt
    17881 gttgtattta tctgcccagg tagagtaaaa tagatcatta tctaaaaggt aattgttcat
    17941 actatataag tcatttgaaa gtaaaaagat actgtaaata aaaaatgtgg ttaaataatt
    18001 ctcataatca gtgtaagcgt tatcaaaaat atagcatact tgctttgtag atgaatgaca
    18061 cgatatatgt ttggtatgtt ctgcaagaca gatgaatatg gaggtggaga caatgtcttc
    18121 caaagaacta acaatgtatt tagagaaaga attggatcaa ctgaaagaaa aggggttata
    18181 caatacgatc gacatactag aaagtgaaaa tggtgcttgt atcatcgtag acggaaaaaa
    18241 gatgatcaat ctagcatcca acaattatct tggatttgcc aatcgcgaag aattgaaaaa
    18301 agcatgtatt gaagcaaccg aaacttatgg tgtaggagca ggagcagtcc gaacgatcaa
    18361 tggttcgctg aaaatccatc agcaattaga agaaaaaatc gcagaattca aagggacaga
    18421 agcagcaatc gcctttcaat ctggattcaa ctgcaatatg ggcgctatct ctgcagtaat
    18481 gacaaaagaa gatgctatct tatcggatga attaaaccat gcatctatta ttgacggttg
    18541 ccgcttatcc ggagcaaaaa tcatccgaat taaacatcaa gatatgaaag accttgagaa
    18601 aaaagcaaag gaagcgatag aaagtaagaa atataaaaaa atcatgtata tcacagatgg
    18661 tgttttctcc atggatggag acattgcacg attgccggaa attattccga ttgttgaaaa
    18721 atatggactg atcacctacg tcgatgatgc gcacggctca ggcgtaactg gaaaaggtgc
    18781 aggaaccgtc aagcattttg gattaagcga caaaatcgat atgcaaatgg gcacgttatc
    18841 gaaagctata ggtgttgttg ggggttatgt agctggatcg aaaacactaa tcgactggtt
    18901 gaaagctaga agtcggccat tcctattttc tacttcgcta actccaggtg cagctgcagc
    18961 tgcattggct tcgattactt tgatgcaaga acatccggaa cttgtcgaaa aagtttggga
    19021 aaatgctaat tattttaaag aagaattgaa aaaagtaggc tataacatcg gtatgtctga
    19081 aacacctatt actccagtca ttttgggaga cgaaaaagtg actcagactt tctctaagaa
    19141 gctgatcgaa catgggattt atgctaaacc aatcgtttat ccaactgtgc ctcttgggac
    19201 aggcaggatc agaaatatgc cgactgcgga acatacgagg gaaatgcttg atgaagcagt
    19261 agcagtttat caaaaaatag gaaaagaaat ggaaattatt taactaaatt tagttttttc
    19321 gtctatttaa aattagagaa catgttcacg catatattgt taatcatctg aagatagtag
    19381 taaaaaagat cagggaggat catcatgaaa aaagtattgg tgacaggatg tttaggacaa
    19441 attggttcag agttgatcac acggctacgt caagaactag gcagtgaaaa tgtcattgcg
    19501 acagatatac gcatgcctga agtcattgag aaaaacgata tctttgagat cctagatgta
    19561 atggattacg aaaaaatgcg cagcatagca gaacaatatc aagtggacac attgattcat
    19621 ttggctgctc ttttgtcagc tgttgcagaa gcaaaaccac aattagcctg gaatttgaac
    19681 gtgactgggc tggtcaatgc attggaagtc gctagagaat tccagctgaa attttttacg
    19741 ccaagttcaa tcggcgcgtt tggtccaaat actccaaaag atcatacccc acaagatact
    19801 cttcagcgtc ctacaaccat gtatggggta acgaaggtag ctggagaact actatgtgat
    19861 tattattatg tgaaatatgg cgtagatact cgtggtgtcc gtttccctgg attgatttct
    19921 tatgcaacct tgccaggagg cggtacgaca gactacgcag tcgatattta ttattcagca
    19981 atcaaagaag gaaagtacac tagtttcatt gatcaaggta catcaatgga tatgatgtat
    20041 atgccagatg cgatcgatgc cattataaaa ctcatgaatg cagatcctga tcgattggtt
    20101 catcgaaatg ccttcaatat atcggcgatg tcttttgacc cggaagagat caaagcatct
    20161 attcaaaaaa tcataccaga ttttgaaatg gattatgatg tggatcctgt acgtcagtca
    20221 atagcagaat cttggccaaa ttcactagat gtttcttgtg cgcaagagga atggggcttt
    20281 tcgccgaaat acgacttaga tgtgatgaca aaggatatgt tggaaaaact gaaagaaaaa
    20341 ttggtagaga cggtataaaa caagaaccac atccaaaaag ttttttatac ttttgggatt
    20401 ggctatggta tgaaagggga caagggatgg aagaatatca agccacgcca gtaaaagaag
    20461 taaaaataga aaatgaatta aaacgtacga tgggattttt tactgcttta tcgactgtta
    20521 tgggcacagt gatcggtgca ggggtatttt ttaaagccgc aagcgtagca gaagttacag
    20581 gatccgccag tttgcatatg ttttcttggt ttctcggtgg tatgatttct gtctgtgcag
    20641 gactaacagg agctgaactg gcagctgcta ttccagaaac aggagggatg attaaatata
    20701 tcgaacggat ttacggaaat acagcagctt ttttattggg ctgggcgcaa gtcgtgattt
    20761 attttccagc aaatgtagca gccttgtcga tcattttcgg tacacagttc gtcaatctat
    20821 ttggtctttc ccagtcaatg attgttcccg tagctgttac agctgctgta tcgatcctgt
    20881 tgatcaattt tttaggttct aaagctggag gggcttttca gtcgattacg cttgtctgca
    20941 agcttattcc tttgtttgtg atcgtcattt tcggtttgtt ccgacaagaa ggagtagact
    21001 tccaactgtt tccgatccaa gcaggagaaa atctttcatt tttctcagcc ttgggagcgg
    21061 gcttgctagc aacgatgttc gcatacgacg gttggataca cgtaggaaat atttcgggag
    21121 aattgaaaaa gccggcgaaa gatttgccaa aagcaatttc tttaggaatc atcggaatca
    21181 tgatcgttta tttattagta aatgcggtgt tcttaagaac cgcatctatc gacggagtcg
    21241 caggaaacag caatgcggca agtgacgttg caaaaatgat ttttggcggc ttcggtggaa
    21301 gactggtcac agttggtatc ttgatttctg tttatggaac catcaatggc tataccctta
    21361 ctggaatgcg tctcccttat gttatggcaa aagaaaacaa cctgcctttt agcaagctct
    21421 ttgctaaact tcatgataaa actaaagttc ctgtagccgc aggtatctta gagctagtca
    21481 tcgctattgg catgatgatg atcggtggtt ttgatacact aacggatatg ctgatctttg
    21541 tcatttggat tttttatacg atggtctttg ttggtgtcat tcttcttcgt aaaaaagaac
    21601 ctgatttgtt tcgaccatac aaagttccaa tgtatccgtt tattccacta gtagcaatca
    21661 ttggagggac ttttatcgta tcaagcacgc ttatcactca gaccttttta gcttcgatgg
    21721 ggatagtatt gacacttgct ggtatcccga tttatctcta tttaaaacgt agatagctaa
    21781 aacattcgaa ttttattaga actgcacttc tctgccaaac aaaatatttt atttaaatga
    21841 ggagtacgtg gaagaaatgt caaaagtact cgttaatttt gatcacgtaa atgtcgtttt
    21901 gtctacgatg aaatagcaaa gtaaaagaca gaaatcgggc tcaaccgatt tctgtctttt
    21961 actttgattt gcgtaactga actattcggc gtacgccgcg ataaatattt aataaaacca
    22021 agcaaccaga aatgaaaatg ggaagccaaa tcagatagac agggtagcca agcactttag
    22081 acaaagcatg tggagtcatt ttatccattt caagaagtgc ttgctgccaa gtctgaagat
    22141 tgaagactag catgaaaccc gaaataatca ggagaataaa tcctaaataa tgaattgtag
    22201 ttctttttct tacataagcg atcgtagcat aaaggaacag taaaaaaata ggtacgccaa
    22261 aaacaaagta catattcagc atctcgacac cccctttgtg tttatcttac tttactttga
    22321 aagaaatgag aaataatagc ctcgttactt gcttgaaaca gcaaaggact gctaagataa
    22381 gagagataca aagaatcaag gaggagtcat atggcaagtt atatcgaacc gattcgtttt
    22441 gcgctgattg tttttccatt tttagcctta gcgatctcat ctgttttttt tatttatgaa
    22501 tatcgaaagt atggaacatt tctagtaaca agggcagtta ttttgtattc tttcgttttt
    22561 tatttattgt gtgcgtattt tttggtgatc ttaccacttc cggcaaagga gacggttgcc
    22621 caaatgacag ggccaaagat ggagctgcat ttatttgctt catggcaaca tttcagagac
    22681 aacacggtat tggtcttgtc tgaacctagt acctacctgc cggcaatgaa gcagagtgta
    22741 tttttggaac cagcttttaa tgttttatta ctcataccat ttggcattta tttaagatat
    22801 tatttccggc tttcttttat gaaaacgatg attgctagtt tctgcctttc tctatttttt
    22861 gaattgacgc agctaagtgg actttatttt atctacccaa gaccataccg cttatttgat
    22921 gtgaatgatt tagtgacaaa tacattgggt ggaatcattg gttttgtcgt tacgccgtta
    22981 tttacattta tgcttcctac aagaagacga atggatgaac tttcttatga aaaaggaaag
    23041 actgtctcat tgactcgacg cttggtcgct tatttgatcg actggacatt tttatctctt
    23101 attaatttgg tagcggcgat tttctttaaa attgcgttcg attcaggggc agttacttct
    23161 catatctggt ggcttttatt agaagtaggg gtatacttta tgattgtacc gtttataacc
    23221 aatggatgga cacttggcaa aaaaattgtg cgtatccgtt tggtggaaga agaccgcgaa
    23281 cgaatttctt taaaagcatt gatgatccgc tacgggtatc tgtactttct tttttatggt
    23341 ttaagctggt tggggcagtt gcttcagcaa atcatgaatt cgacaacgaa tacggtgttg
    23401 cagatgagtg cagtattcat ttctctcggt gtcttgttac tacaaggtat ttttatttta
    23461 aatatcttgc tatcattttt gagaaaaaaa cggattctgt tttatgaaaa agctagtcac
    23521 acatatactg ttagcacgat tggccaaaca aaggatgatg attctcgaaa ttagctgagc
    23581 aaaaacgctt tgtctgtgtt ataatgccaa agtcgtgata atttaaagag aagaggtgca
    23641 cgaatgaata attttgatat attccgcttc aaaccgttta tcaatgaagc gttaaccgca
    23701 aagggcttca aacagcccac agaggtccag gaacgtttga ttcctttaat tgcaaaagga
    23761 aaaaatatca ttgggcaatc tcaaactggt acaggaaaaa cacatacgtt tttgttgcct
    23821 ttgtttgaaa aaatcaatgc agaaaaagag gaagtacaag ttgttattac agctccaagt
    23881 agggaactag ctgcacagat ctatgaagca gctacacaaa tcgctaaatt ttctacaccg
    23941 gagatccgtg tctcgaattt tgtcggcggt acagacaaac agcgtcaatt aaatcgtctg
    24001 catcaccaac agccacaggt tgtagtaggc acgcctggta gaatattaga catgatgaat
    24061 gaacaagcgc tgaagattca caccgcattt gcatttgttg tcgatgaagc ggatatgaca
    24121 ttggatatgg gttttttaaa tgaagtagac caaattgcaa gtcgattacc agaaaagctg
    24181 cagtttttag ttttttctgc cactattcca gaaaaacttc gtccattctt acgaaaatac
    24241 atggaaaatc caatcattga agaaatcaaa ccaaaagcag ttatttctga agacattgaa
    24301 aattggctga tttcaacaaa aggtaaagat gagaatcaaa tgatttatca actgctgaca
    24361 attggtcatc cttatttagc aatcgtattt gccaatacaa aacagcatgt ggatgaaatt
    24421 gccgattact tgaaaaacca aggcttgaaa gtagcaaaga ttcatggaga catcacccca
    24481 agagagagaa aacgagtgat gcgacaagta cagaatctgg agtatcaata tgttgtagcg
    24541 actgatttag ctgctagagg gatcgatatc gaaggtgtct ctcatgttat taatgcagaa
    24601 attccagaag atctagactt ctttatccat cgtgtcggac gtactggacg taatggctta
    24661 aaaggaacgg ccattacgtt atattcgcca aatgatgagc aagccattga tgcgatagaa
    24721 aaattgggcg tctcatttga accaaaagaa atcaaaaacg gggaaatcgt caaaacatac
    24781 gatcgtaata gacgtgccaa acgagaaaaa tcaaaagatg tattagatcc cacgctgatt
    24841 ggtcttgtga agaagaaaaa gaaaaagatc aagcctggtt ataagaaaaa aatcgattgg
    24901 gcaattgcag aaaaaaataa aaaagaacgc aaaatcgaac gcagacagca aagccgtact
    24961 gctcgtaaaa ataagaaaaa ttcacataaa taaaaaacaa gaggttgtga caaaaggcgt
    25021 gtcacaatct cttgttttta agctagactt tttttcattc cgatacaaag tacttcatct
    25081 tcaataaaag gttctgtgca agtatgatac ccttgttttt cataaaaatc tacagccgtc
    25141 atttcagcta ctacataact agtctgacaa ccttctgaaa ccgcccgttt ttcagcagct
    25201 tcaagcaact gaccgcctaa cccttttcct cggtattctt ttgctacgca aaatcgttcc
    25261 ggcttgaagc aagtaggtgt tttcttttca taacgcaaag tagctactgg tcttttttga
    25321 tcaagaaata gataataggt ttgttgcggg atatcttttc cgtcgaattc tagttctgca
    25381 ggaatccctt gttctaatac aaaaacttga tatctcaaaa agcagctagc tgcgcgtatc
    25441 cattcatccg ctccaactat ttttttcatc atattcctcc ttgtcatgaa tagcagagat
    25501 gtattgattc ctttattctt atcgtaccaa aaatcgtgga gtatgaccaa aaggaaatga
    25561 aaataaaaat tgagatattg acagtgatca atagaatgcg tatactaaat aagtacgaaa
    25621 aggatatgat ttgtttttta agactcacag aaaattcttt cttggctgaa aaaagaatag
    25681 tcactaatca aattgctccc tttttctatt ttggtagaaa taccgacgca gctcagcgtt
    25741 atcgagttta agaggtaatg attacacatc attgcaatca aggtggtacc gcgagttttc
    25801 gtccttggct gtgatggtgt ttttttgtat ttaatttata gaaagcaggt aatgacatga
    25861 aacaattaag tagtgcacaa gttcgacaaa tgtttcttga tttcttccaa tctaaaggac
    25921 atacgattga acctagtgcg tctttgatcc ccgtgaacga tccaacctta ttgtggatca
    25981 actctggtgt tgcaacattg aaaaaatatt ttgatggttc agttgtgcca gaaaatccaa
    26041 gaatcacaaa tgcccaaaaa tctattcgaa caaatgatat cgaaaatgtc ggtaaaacag
    26101 cacggcacca tacgatgttt gagatgctgg gtaatttttc gattggtgac tatttcaaaa
    26161 atgaagccat ccactgggca tgggaatttt tgactgatcc aaaatggatg gcattcgatc
    26221 cagaaaaact ttatgtaaca gtttatccaa aagatacaga agcaaaacgc atctggcatg
    26281 aagaagttgg cctgccacta gatcacatta ttgatgtaga agacaacttc tgggatatcg
    26341 gtgcaggccc atgtggtccg gatacggaaa ttttctatga ccgtggagaa gcttataacg
    26401 atttagcaga agacgatcct gaaaactatc caggaggaga aaatgaacgt tatttagaaa
    26461 tctggaactt agtattttca gaattcaacc ataagcctga taacacattt gaaccacttc
    26521 ctcataaaaa cattgatacg gggatgggat tggaacgcat ggtctcaatc gttcaagatg
    26581 cgtcaacgaa ctttgagaca gacttgttta tgcctattat ccacgccgta gaaaagctaa
    26641 gcaaacaagt aaaatatggt gaaaacccag taacagatat ttcttacaaa gtaatcgcag
    26701 atcatattcg cgccctttca tttgctattg gcgacggtgc attgccatcg aatgaaggga
    26761 gagggtatgt cttacgccgt ttgcttcgtc gtgctgtcat gcatgggaaa aaattaggta
    26821 ttgaacaagc attcttatat cgtttagtcc ctgttgttgg ggaaatcatg gtaagttatt
    26881 atcctgaagt acttcaacaa aaagaattta ttgaaaaagt tatccgtaca gaagaagaac
    26941 gtttcaatga aacaatcaat gaaggtttgg aaatcttaaa tcaagtgatc caagaagtaa
    27001 aaaacgaaaa cgggacgact ctagcaggaa aagacatctt caaactatac gacacatatg
    27061 gcttcccggt agaattaacg gaagaggtgg cagaagatga aggactgaaa gttgatcatg
    27121 ctggttttga aaaagaaatg gatgcacaac gcgaacgtgc tcgctctgcc cgcagtactg
    27181 aaaaatcaat gggggtacaa tctgctttat tgacagatat caaagtagaa agtaaattca
    27241 ttggatacac agaacaagaa tcagatagtc aattattggt catcgttaaa ggtgagtctc
    27301 ttttgaatga agtcgcagaa ggtgaagctc aaatgatctt tgatcaaaca cctttctatg
    27361 cagaaatggg tgggcaagta gcggatcaag gatggatcat gaatgaagta ggcgaaatcg
    27421 tagcagttgt tacagatgtt caaaaagcgc ctaatggtca atttatgcac accgtagaag
    27481 taaaagcacc aatgagtgaa ggacaaacct atcatctatt gatcgatcaa tcgatgcgca
    27541 atcggatcat caaaaaccat acagcaaccc atctactgca caaggcattg aaagaagtac
    27601 ttggcgagca tgcaaatcaa gctggttctt tagtcgctcc tggacattta cgattcgact
    27661 ttacccactt tggtcaaatc acttcagatg aattagctga aatggaaaga atcgtcaacg
    27721 aaaaaatctg ggaagctatc ccagttgaaa caattgaaac agatatcgat acagccaaaa
    27781 acatgggtgc aatggctttg tttggtgaaa aatacggaca tgacgttcga gtagtaaata
    27841 ttggcggctg gtcaatcgaa ctgtgcggag gcacacatgt agcaaataca gaaaacatcg
    27901 gaatcttcaa aatcgtttct gaatcaggaa ttggtgcagg cgtgcgacga atcgaggctg
    27961 taacaagtaa agaagcgtac gaactacttg aagaagaaga aaaacaacta aaacaagtag
    28021 caacaattgt taaatcacct caattgaaag aagttgtttc aaaagcggaa caattgcaac
    28081 agcaagtaag agagctacaa aaggaaaatg aacaattagc tagcaagtta gcgaatcaac
    28141 aagctggtga tgtattcaaa gatccagaaa cgatcaatgg aaaaacaatc attgcagcac
    28201 aagtaaaagt gaaagatatg aatcagctgc gtcaattagc tgaccaatgg aaacaaaaaa
    28261 atatttcaga tattcttgtt ttagcaaacg aacaagaagg aaaagtcagt ctgctagcgg
    28321 caatgacgaa agaatcaaat gaagctggac taaaagctgg cgacttgatt aaagcaatcg
    28381 caccgaaagt aggcggtggc ggtggtggac gtccagacat ggcacaagct ggcgggaaaa
    28441 atcctgctgg tattccagat gcacttaaag ctgcgaaaga atgggttgcg caacaatagg
    28501 gcgtttacgt ttgacagaaa aacaaaagtt caaacattga acgaaacatg attatggggg
    28561 cgaagacaaa caagtcttgc cctcatactt ttttcaattg gatggcgcat ctaatcaaat
    28621 ggtcggattc ggatggtctt tagttgacga aagtgacaga aatttttacg attagtataa
    28681 ataaaaaggg gtgaaaaaat gaatagtaga aataagacct ttcgactagt cttacgtgcg
    28741 atcttgttag caatcatcat cgtacaagcg atggttcctt ggctggggtt tattccatta
    28801 ggattcatta gtttaacaat tatccatatc acagtaatta ttgctgcagt agtattaggc
    28861 ccaaaagatg gcatggtgat tggcttgttt tggggaatcg ctacaattgt tcgtgcctat
    28921 gccatgccaa caacgccatt tgatacatta gtgttcacga atccgattat ctcggtagtt
    28981 cccagagtac ttgttggctt agtagcaggc ttggttttcc actggattta tcaaaaaaat
    29041 aaatcaataa cgatcagctc agcctttgca ggggtactgg gttcattggt caatacagtc
    29101 ttagtgttag gattcatggg attattttat acaggtgcaa cggcagatgc ctatggtgta
    29161 gactctgctc ttttatttaa aaccttagca ggggttgcag caatcaatgg gataccagaa
    29221 gcaataggtg cgggaatcat aacaccatta ctagcaaaag cgttatttgc agcaacaccg
    29281 ttgaaaccag aataatgacc atcttttaat agtaatagga cgcacgatag atagaaaaag
    29341 gaagagagga gaggaaaaca atcgtcctct ccatcttttt acctgagcaa caagcaaact
    29401 agaaggggta aaaaacttgg ctaagaaaca agaagaaaaa aagaaacaaa taccgcaaca
    29461 gcggacaaaa aaaggagtac tagcgggtat cttaggattg gttacgaatc tgatcttgtt
    29521 tgtctctaag ctaatgatcg ggcttttttc aggaagtgtc tccattatgg ctgatgcagt
    29581 gaacagtcta tcagatactg cttcatccat attgactttg atcggattta aaattgctgc
    29641 aaagccagca gatcaggaac atccttatgg acacgaacgc ttcgaatata tcagtgggtt
    29701 atttgtttcg atcatcatta cttatgtagg atttcaattt ttagagtctt ctgctgaaaa
    29761 aatcatccat ccagaaaatg tctctttaac acccatcgtc ttttttgtcc taatcatttc
    29821 catctttttg aaatttgcac aaggcaaaat gtatcaggtt attgcgcaat ccattgattc
    29881 tgaaacattg agagctacag caacagatag ttataatgat gtctttacta cactggctgt
    29941 attgctttca gcagcaatcg aatgggtaac tggatggcgt attgatggat atataggttt
    30001 tgctttagca ttatatatcc tttatagtgg attgacgatg cttcgaaatt ttgtatacga
    30061 actattagga agtcgcccaa cgccagcaga gattgaagaa atggaagagc atttaaatgc
    30121 ttgcgatgat atacttggct atcatgattt attggtgcat aactatggac ccaataaacg
    30181 ctttgcatca gtccacatcg aagtagatga atcaatgaat ttgaatgtgg ctcataagat
    30241 tattgatctg atagaaaaag actttaaaaa gtctttggat gtggaacttg tctgtcactt
    30301 ggatccaatg cctgttgata acgaaaaata ctataatatc ttgcggcagt taaagcaaat
    30361 tcttcttgaa acaggtacag ggcttaatat tcatgatttt agagtaattc atcaaggaaa
    30421 aacgctgcaa tttgatgtag tggtccctga gaaattccag tttgttgata cagaactaga
    30481 aagaatctta cgtagtcaaa tagaagaaaa aatcggagat taccaattgg aaattacgtt
    30541 tgatcacaat tatcttttat attaaatgag aaactataaa aagactcctt aaagttttta
    30601 atttaaagag tctttttttc ttttaattgt ttatggagag cctgacgtgc aagattatct
    30661 gctcctttat ttttgttttc gggcaaccat tgcaggatca ataaagaaaa ttcggataga
    30721 agttcttgga ttctttgaag gtagggaagg aagagctcgt tcttcgttgc gtttttgtcg
    30781 accgtagaaa ctagtgtttt gctgtctgaa aaacagaaaa tcgtttcatt ctgtaagtcg
    30841 tttttcttta gaaattctaa agtttttaaa aagacttcga actctgcttg atggtttgtc
    30901 cctatagaaa gcgggataga tagctgttct tgtttacctt gatagacaat caagattcca
    30961 cctccactag gaccagggtt gccttttgta gaggcatcaa tgtatacttt taacataaaa
    31021 agagattctc ctttttgttc aaactaatgt tatggtaaga ttatatcatt actgaatcgg
    31081 tgagaggtta gagaatgaaa aaaaatattc gatggcagcc tgaactggca catacgatca
    31141 tctattggtc attgaccttg atcattctgt tttttagtct gattctttct ttagaaaaca
    31201 caggtcctta ttggaaaagc aattttgttt taggtatttt tcttgttttt ctatttctag
    31261 gtttgcggcg ttcttttatt ttaaaatcaa actctgtgat tatacgttat gctgcttttt
    31321 ggaaagaccg agaaatatct gtcggacaga ttcgatcttt gacgccaact aatactgggg
    31381 tgcaaatgat gttgcaagat gagaaagccc catctgtttt catgatgcga aaaaaagatc
    31441 aagtagtcct ttttgatcat tttgaaaaaa acggacccaa atcagaatca attccttatc
    31501 cttatacgaa aaaaagaaaa agctaatttt aaaaatcaga acctaaaaaa acgaattttt
    31561 acaggaaaag aaaagagtga gacgtacggt tcatcccgtt tagcgtctca ctctatttgt
    31621 ttatctattc atgcatacta gtatcggtag tctgaacgtt gcctttcttg acatgggcag
    31681 cttgtgttcc tcgattccct tcgatgatct caaattcgac cacttgattt tcttccaaag
    31741 ttttgaatcc ctcttcttca atcgcagtaa aatgtacaaa aatttcttct gtgtcatcat
    31801 aactgataaa gccgtatcct tttttgttgt caaaccattt tacaattcct gtggtcaaag
    31861 cactcacgac ctttctaaag tctgatcata gccttcatta taaagagtat ttataagtac
    31921 gtcaaataaa ttaaaaacct gtaattttat gttttttttc tgaataaaaa aaggtataat
    31981 tggcatatct taacaaagaa gatattttgt tgaataaagt tcgtgaaaag gggtaatcaa
    32041 atgaagaatg atctgcaaat ttcacaagaa acagagctag aaccgattgt tgaagtggct
    32101 agtaaattag atcttaagga agacgatttg gaactgtatg ggaaatataa ggcgaaaatc
    32161 aatttttctg gaatcaatcg atcaaaggat aacgctgagg ggcatttgat cttagttact
    32221 tccatcaatc caacaccagc tggcgaagga aagtcaacga tcactgtagg gttaggagac
    32281 gcgctcaata aaatcaataa aaaatctgtt attgcattgc gagaaccctc acttggtcct
    32341 gtgatgggaa tcaaaggtgg tgctgcaggc ggaggatatg cacaggtatt gcctatggaa
    32401 gatatcaact tgcattttac tggtgacatg catgctatca caacagctaa taatgcatta
    32461 tctgctcttt tagataatca tattcatcaa ggaaatgaat tgaacatcga tgcccgccga
    32521 gtgatttgga aacgggtagt cgatctcaat gatcgtgaac tgcgtaaagt aattgttggc
    32581 ttgggtgggc caatccaagg tgttccaaga gaagatgggt ttgatatcac agttgccagc
    32641 gaaatcatgg ctatcttgtg tcttgcggca gaccttgaag atttaaaggc tcgtcttgca
    32701 aggatcgttg tcggctatac atttgaccgt cagcctgtga ctgctggaga cttgaaagca
    32761 gaaggcgctc tggcattact gctgaaagat gcaatcaagc cgaatcttgt acaaacaatt
    32821 tacggaacac cagcttttgt ccacggggga ccgtttgcca atatcgctca tggctgcaat
    32881 agtattttag ctaccaagac agcattgaga ttagcagatt acgttgtcac agaggcagga
    32941 tttggtgcag atcttggcgg agagaaattt ttagatatca aagtacctaa cttgaaaaaa
    33001 gcgccggatg ctgtggtgat tgtcgccact gtccgtgcat tgaaaatgca tggcggaatg
    33061 aaaaaagatg aacttaaaaa tgagaatctt gatgctttga aagtaggttt tgcaaattta
    33121 aagcgccata tacgtaatat ggaacaatat cagctacctg tgatcgtcgc aatcaatgaa
    33181 ttcgtaacag atacagacaa tgaactcaca ctgttggagc acttgtgtga aaatcagggg
    33241 attcttgcca agagagcgag tgtatgggca gatggagcag aaggcggcgt tgatttagca
    33301 aaagctgtag tacgattgat tgatagaaaa gaagctaatt acaagccatt ataccgccta
    33361 gaagaaacga tacaagaaaa gacagaaaca atcgtcaaga aaatttacgg aggaaatggt
    33421 gttgttttca gcaaaaaagc gcaaaaacaa atagaagagt ttacgaagaa tggatgggac
    33481 aaactgccga tttgtatggc aaaaacacag tattcatttt ctgatgatcc ttcactgcta
    33541 ggtgcaccgg aagatttcac catcacgatc cgcgaaatca tcccaaaatt aggggcagga
    33601 tttttagtag ccttgactgg agatgttatg acgatgcctg gtttgcctaa aaaacctgct
    33661 gcattgaata tggacgtgac aaatgacgga gaagtgttgg gattgtttta agcttgatta
    33721 taatctacca tcgaaggagc tgtattttga ctacagcttc tttttttaga cgatagggaa
    33781 ttaattttgt ttacactagg atcattcttt ttctttattg tattttgagc tatactaaag
    33841 cataatagga gggtatgatg aaaacgacat ttataagaaa aaccggtttt tacggtatgg
    33901 gtagctcgat tcagctaaga atcaacaatg aaaaacgcag tgttctcagt catgaacaaa
    33961 agataacgct tgatctggaa gtgcccttta cgatgcaggt caccttttat tggctaaaaa
    34021 gccccgttta tacagtgaaa gaagcaaagc ctgcttatgt gatcacaatg aattacttga
    34081 ttcttcaaat ctatccgttt ttatttctag caactgggtt gggggccgtt ttcctacaaa
    34141 atttattttt cagttttttt gcagttattg tgttgattgc gttctttttc tatataaaaa
    34201 accgggcata tttgatcaag gaggaaaatt atggcaaact ctgatctttt tttaatcagt
    34261 ttcaatcgaa tcgaaaaatg gatgagagac gagatgggaa atgcacgaaa tatgggattt
    34321 accgaacttg tcagacgcct agctcaaaga aaacaattaa tgatcagaaa atacgaagat
    34381 gatcttttac agcttgctca gttaaggaat gcgatcgtcc atgatcggat cgcagtcgat
    34441 tttgtcattg cagagccaaa tgattggaca gtcaagcgaa tccagctgat cgaacaagaa
    34501 ctgattcagc ctgaaaccgt gctgcctcga tttgcaaaac gtgtaacagg ttttgaacaa
    34561 gatcttccgt tacttgaact gttaaagata gtagcagaaa aaaggtattc acaatttccg
    34621 ttatacaata aaggaaaatt tgtagcattg attactttac gtaatatcgg gttttggcta
    34681 gcaaaagaaa gccaaaaagg tccggttgat ttaacaaata aacgagcgtt ggatttgatc
    34741 atccaaaacg gaaagtacac aaattatcat tttgttccag ctaaaacaca tatttacgag
    34801 gttgaagcga tgtttcgtga gcaagggact ttggaagccg tacttatcac taaagatggg
    34861 aatcccgatg gtaatctgct cgggatcgta cgacctcgtg atatttataa acaaatagaa
    34921 aaggattgat tttttgttaa ttatttattg gatattcagc gctttgctag taggagtgga
    34981 tcagttggtc aaatggtgga tcactgataa tctagcgctt ggagagacga aaaatcttat
    35041 accaggtatt ttgtcactga atcatattcg aaacactgga gccgcatgga gtatgctaga
    35101 agggaagatg tggttcttta ctatagtgac actcattgca gtcgttgtga ttctcacatt
    35161 gatgattaaa aatcgtgaaa aaggtaatcg ctggttcatg attggattga gtttgattct
    35221 agctggtgcg attggcaatt ttatcgaccg tattcgtctt ggctatgtag tagatatgtt
    35281 ccagacagat tttatgagct ttccgatttt taacgtagca gatgtgacac ttgtatgtgg
    35341 agtgatttgt atgttaattt atattatttt agatgaaaag gatcaaagaa aaaaatgaca
    35401 gaacttaaaa cgacgattca ccatgaatct ggtaggatcg ataaagtatt gactaattta
    35461 ttcaccgatt acagtcgttc gcaaattcag ctgtggctga aaaatggagc ggtttctgtc
    35521 aatggacagc cagtcaaagc aaattacaaa gtaaaacaag gagacgagat tactgtcttg
    35581 atccccgaac cagaaacatt agatgtggtc ccagaaaata tcccgttgac gatcgtttac
    35641 gaagatgaag atgtagcggt gataaacaaa cctcaaggaa tggtcgttca tccttcagca
    35701 ggtcatacgc atggcacaat ggtcaatgcg ctgatgtacc atatcaagga tctttctacg
    35761 atcaacggcg ttattcgtcc aggtattgtt caccgaatcg acaaagatac ttccggactg
    35821 ctaatggttg ccaaaaatga ccaggcacac gaatcattag ctcagcaatt aaaagataag
    35881 acctctttgc gcaaatatgt tgctttagtc catggagaga ttcctcacga aaaaggaacc
    35941 atcaatgcgc caatcggccg ttcgaaggaa aaccggaaaa tgcaggcgat aagagaagat
    36001 gggaagtcgg ctgtcacaca ttttactgta ttggaacgtt tcgaaggatt tactctggtt
    36061 gaattaaaac tagaaaccgg aagaacacac cagatccgtg tgcatatgaa gtatatcggt
    36121 tatccgttag ctggagatcc agtatacgga ccgaagaaaa ctttgaaagg caatggtcag
    36181 tttctccatg cgcaaatgtt aggttttacg cacccgacta caaacgaaaa gatgatcttt
    36241 gaagcacctc ttccagaagt ttttgaaaaa actttagaaa aattaagaaa aaacattgca
    36301 ttttaattta gataacggta tagtaattaa caacgaaagc ataaaactta atagaattcc
    36361 tttaagttta gtcccgtgag gctaagaagg gtgggagcaa ggatacgtat ggtgcgccat
    36421 aagtagattg ctcattgaca tagtatagct atcagtgtat cagtatacgc tcagtctaaa
    36481 cattcctcct gtcctcttag ggcaggagtt tttttatgca aaaaagtagg gggaatgaaa
    36541 atgcaagcaa aagaagtagt agatcaggta accatgaagc gagcattgac tcgaattact
    36601 tatgagatta tcgagagaaa ccatagtatc caagatatcg tattggtagg tatcaaaaca
    36661 cgaggaatct acattgcatc aagaattgcc gaacgcctga agcagctaga agatatcgat
    36721 attcctgtag gagaactgga tatcacttta taccgagatg acaaaaaaga aaatccagaa
    36781 gaaccggaac ttcattcttc tgacatccca gtttccttag aaggaaaaga agttattttg
    36841 attgatgatg ttttgtatac aggacgaacg attcgagcag caatggacgc agtaatggat
    36901 tttggcagac caagaaaaat ttcattagca gtcctggtcg atcgtgggca tcgagaattg
    36961 ccaatccgtg cagattatgt tgggaagaac attccaacat ctcgggcgga agagatttta
    37021 gtagagatgc aagagcttga tggacaagat cgcatcatga ttttgaaaga ggaagattag
    37081 aggagagaga aacatgtcgg agaaaaaagg atttcataat gatgacgtgg tgttagatat
    37141 ccacgatcgc ccaaccccag gacattgggt aggattaagt ttacaacact tgttcacgat
    37201 gttcggagca accgtgttag tccccatctt agtaggtatc gatccaggta ttgcgctagt
    37261 cagttcagga ctaggtacat tggtgtactt atttactact aaaggaaaga tacctgcgta
    37321 cttaggaagc agcttcgctt tcattgcagc gatgcagctt ttgatgaaaa cagatggtta
    37381 tccagcgatt gcacaaggag cagtcacaac tggattagtc tatttgattg ttgcgctgat
    37441 catcaaaaag atcggttcag catggctgga taaaatcttg cctccaatcg tcgtcggtcc
    37501 agtcatcatg gttatcgggt tgggacttgc ggcaaatgca gcaacaaatg ctatgtataa
    37561 tcagggcacg tatgatttca agtatattgc tgtagcgctg atcacattag gattaacaat
    37621 tttcttcaac atgtggctaa gaggttttct cggattgatt ccgattttac taggtattat
    37681 tagcggatac atcgtagcct tgctgtttgg aatcgtggat actaagccaa tcgtagacgc
    37741 tgcctggttt gctgttccta actttgagat tccctttgta caatatgaac caaagctata
    37801 tcttggagca atcacaacaa tggcacccat cgcttttgtg acgatgacag agcatatcgg
    37861 ccatcttatg gtattgaata aactaactaa acgtaatttc tttgaagaac cgggtcttca
    37921 taagacgttg acaggtgatg gattagcaca aatcgtggca gcctttgtcg gaggtcctcc
    37981 tgtaacaagt tacggtgaaa acattggtgt tctggctatc acacgtgtcc atagcgtgtt
    38041 tgtgatcggt ggagctgcag tatttgcagt cattttagga ttcgtaggaa aattaagcgc
    38101 tttgatttta agtatccctg gaccagtcat ctcaggaatc agttttatct tatttggagt
    38161 aattgccgca agtggaatga agattttaat cgagaacaaa atcgattttg ataaaaagaa
    38221 aaatctgttg attgcttcag ttatcttagt agtaggaatt ggcggactcg tctttgaagt
    38281 aggcacattt acattgtcgg caatggcatt agcaactgta cttggcatca ttttaaatct
    38341 tatcttacca gaaacttcac gaagcgaaga aaactaagga ggaaacatta tgatcatcaa
    38401 atcagaacgt atcagcttaa agcatttatt gaccgttgag gcacttactg atcaagaagt
    38461 tatgggcttg atccgcagag ggcaggaatt caagcaaggt gctaattgga ctccgcaaaa
    38521 gcgccagtac tttgctacaa acctcttttt cgaaaatagt actcgcacac ataaaagctt
    38581 cgatgtagcg gagaaaaaac tagggctaga agtgattgaa tttgaagcaa gcacaagctc
    38641 tgtccaaaaa ggggaaacac tatacgatac agtactgacg atgtcagcat taggcgtaga
    38701 cgtagcggtt atccgtcatg gtgatgagaa ctattatgac gaactgatac aaagtaaaac
    38761 aatccagtgt tcgattatca atggaggaga cggaagcgga cagcatccaa cccaatgctt
    38821 gcttgatttg atgaccattt atgaagaatt tggtcatttt gaaggtctga aagttgccat
    38881 cgtaggtgat atcactcact ctcgcgtagc taaatccaat atgcagatgt taaaacgtct
    38941 aggcgcacaa gtattcttct caggtcctag agaatggtac gacgatgaat tcgaagtgta
    39001 cggacattat atgccattgg atgaactact agatcaagtt gatgtcatga tgatgcttcg
    39061 ggtgcaacac gaacgccatg atggaaaaga aagtttctca aaagaaggct accatcagga
    39121 acatgggtta acagttgagc gtgcgaagaa gttgcaaaaa catgcaatca ttatgcaccc
    39181 agcaccagtt aaccgtgatg tagaattagc tgattcatta gtggaaggat tacagtcgcg
    39241 gatcgttcag caaatgagca atggagtatt cgtcagaatg gcaatcttag aagcagtgct
    39301 aaatggaaaa gcgtaattat aggaggatga aaaaatgaaa acactcatta aaaacggtca
    39361 gatcaataca cgaaaaaata tgacgacacc agcagaaatt tggatagaag atgggcgaat
    39421 caaagcaatc ggtacaggat tctcagaagc cgaatttgat gaagtgtttg atgcaaaggg
    39481 acaattgatc acacctggtt tagtggacgt ccatgttcat ttgagagagc caggatttac
    39541 atacaaggag acgatcgaag ctgggactcg ctcagctgct cgtggcggtt tcactactgt
    39601 atgcgcgatg ccgaatttaa atcctgtacc tgatacggca gaaaagctga gacaagtata
    39661 tgacatcatt agaaaagatg cagttgtgaa ggtattgcag tatgcgccaa tcacagaaaa
    39721 tctgcgaagt gagaaattag ttgatcaaga agcactgatt gaagaaggtg cttttgcttt
    39781 cacaaatgac ggtgtaggtg tccaaacagc tgggacgatg tatctggcta tgaaagaagc
    39841 tgcaaaaaac aataaagccc tagtagccca tacagaagat gaatctttat tatttggcgg
    39901 cgttatgcac gctggcaaga aggcagaaga acttggtctt cccgggatct taagcgtaac
    39961 cgagtcttct caaattgcta gagatctgtt gctagctgaa gcaactggtg ttcattacca
    40021 tgtttgccat gtttctacta aagaaagtgt ccgtgtaatc agagacgcaa aaaaagcagg
    40081 aatccacgta acagcagaag tttcacccca tcatcttatt ttgatcgacg aagatattcc
    40141 cgaagatttc ggcttttgga aaatgaaccc gccgctaaga ggacgggagg acagagaagc
    40201 tttgatcgaa ggcttgcttg acggtacgat cgattgcatc gctacagacc atgcccccca
    40261 tggattagaa gaaaaaagcc aaagtttcat gaaatcacca tttggtatcg taggaagcga
    40321 aacagcgttt caactgatct atactcattt tgtagaaact ggtcgattca cgttggaaca
    40381 agtgatcaat tggttggcag ttaaaccggc tgagattttt ggtttgaatg caggaacgct
    40441 aacggttggt gcccccgctg atgtagcagt ttttgatatt acacaaactt gcacgatcga
    40501 taaagaagac tttttatcaa aaggggagaa tacacctttt atcggctgga aagtaaaagg
    40561 cgaaacacaa atgacttttg tcaacggaaa acttgtgtgg cagaagggag aataaaagta
    40621 tggaacgttt gcttatatta gaagatggaa cagttttcga aggaaaggct tttggtgcag
    40681 atgctagtgt cttcggagaa atcgttttta caacaagtat gactggttat caagaaacta
    40741 tcactgatca aagttttaac ggtcaaatca ttacatttac ctatcctatg gtgggtaatt
    40801 atggggtaaa tagagatgat tatgaatcga tttctccaac atgcaaagga gtagtagtaa
    40861 aagaacatgc acgtgtagct tcaaactggc gaaaccaaat gacgctagat tcatttttga
    40921 aacaaaaagg aattcctgga atatcaggta tcgatacgcg cgcccttact cgcaaaatcc
    40981 gtgaagtcgg aacgatgaaa gctagtatag ttgatgctgg agattccttc gaacatgcat
    41041 ttgatcagct gaaagcaacc gttctagcaa ccaatcaagt gcagcaagtc tcaactaaca
    41101 aaccttatcc aagtccagga acaggtcgga aagtcattgt cattgacttc ggcttgaaac
    41161 atagtatttt acgagaattg agtaaaagaa actgtaacct cacagtactt ccctataaca
    41221 cagatgccga gacgatttta gcgctatcac cagacggagt catgctgacg aatggacctg
    41281 gtgatccaaa agatgtgcca gaagcaatcg aaatgatcaa aaaaatccaa ggaaaagtac
    41341 cgatttttgg tatttgtctc ggtcaccagt tatttgcttt agccaacggt gcagatacct
    41401 ataaaatgaa attcggacat cgaggattaa atcacccggt acgagaagta gcgaccggaa
    41461 gaattgattt tacttctcaa aatcatggct atgcagtcga tgaagccacg attgatccag
    41521 aaaaactcat cgtaactcat gtagaagtaa atgacggaac agtagaagga gtcaaacaca
    41581 gagattatcc cgctttcagt gtgcagtttc atccagatgc tgcccctggt ccgcatgatg
    41641 cactacattt atttgatgaa tttatggaaa tgatggacgc aggaaaggag cagtaaacag
    41701 atgccgaaac gtacagatat caagaaaatt atggtgattg gatctggtcc gatcatcatt
    41761 ggtcaagccg cagaatttga ctacgcagga acacaagcct gccttgcctt aaaagaagaa
    41821 ggatatgaag tggttttagt aaattctaat cctgcgacga tcatgacgga taaagaagtg
    41881 gcagatcaag tatatatcga accgatcaca cttgaatttg tatcaaggat tcttcgaaaa
    41941 gagcgtccag atgctctcct tccaacttta ggcggacaga caggcctcaa tatggcaatg
    42001 gagctggcag catcaggtat tttagaagaa ttgaacatag aacttctagg aacaaagctt
    42061 tctgcgatcg atcaagcaga agatcgtgat ctattcaaac agttgatgaa agaattgaat
    42121 cagccaatcc ccgagtctga aatcgtcaat acagtagaag aagctgtaga ttttgcaaat
    42181 aaaatcggct atcctgtcat cgttcgtcca gcctttactt taggcggaac tggtggaggt
    42241 atgtgtgaaa atgaagaaga attacgtatc gtatcagaaa atggattaag tctttccccc
    42301 gctacccaat gtttgattga acgaagtatt gcaggtttta aagaaattga atatgaagtg
    42361 atgagagatt cggctgataa tgcaatcgtt gtctgcaata tggaaaattt tgacccggtg
    42421 ggtatccata caggtgattc tatcgtattt gcccctagtc aaacattgtc tgaccatgaa
    42481 tatcaaatgc ttcgtgatgc ttctctttcg attattcgag cacttaagat cgagggaggc
    42541 tgtaatgtcc aactcgctct tgatccacat agttttaatt attatgtgat tgaagtcaat
    42601 ccgcgtgttt ctcgttcatc tgcgttagca agtaaggcga ctggctatcc gatcgctaag
    42661 ctagctgcta aaatagcgct tggattaaca ttagacgaaa tgaaaaatcc tgttacagga
    42721 acgacttttg cagaatttga acctgcatta gattatgttg tagctaaaat tcctcgctgg
    42781 ccgtttgata agtttgaaaa cggagaacgg cttttaggca cgcagatgaa agcaactggt
    42841 gaagtgatgg ctatcggtcg gaatatcgaa gagtctttat tgaaagctgt gcgttcttta
    42901 gaaatcggta cacaccatat cgaacttcct gagttggcag acatcacgga cgatgaccta
    42961 atccaaaaag tggttcgagc acaagatgat cgcctgtttt atgtagctga agctattaga
    43021 agagggtaca ccatagaaga attagcggat ttgacaaaaa tcgatttatt ctttttagat
    43081 aaactgttgc atatcgtaga aatcgaacag cagttgaaac agcagccgtt ggatgacaag
    43141 atattgaaaa cagcgaaaca aaacggtttt acagacaaaa aagtggcgga gctgtgggaa
    43201 ataaacgaag aagccgttcg ccagatgaga aaagaaaatg atttgattcc tgtatataag
    43261 atggttgaca cttgtgcagc ggaattcgaa tcaaaaacac cttattttta tagttcctat
    43321 gaatatgaaa atgaaagttc acgatcagaa aagccctcaa ttctagtact gggatctggt
    43381 cctatccgta tcggacaagg ggttgaattt gactatgcga ctgtgcattc tgtcaaagca
    43441 atccagcagg caggatacga agcaatcatc atgaacagca atcctgaaac agtttcgact
    43501 gacttttcta tttcggataa attatatttt gagccattga ccttcgaaga tgtgatgaat
    43561 gtgatcgagc tagaacagcc gataggagta atcgttcaat ttggtggtca gacagcaatc
    43621 aacttagccg aacctttgac tgctgctggt gtaaaaatct tagggacaac gatcgaagac
    43681 ttggatcgtg cggaaaatcg tgatttattc gagcaagcgc tgaaagagtt ggatattcct
    43741 caacctttag gggatactgc tacaagtaaa gaagaagccg tggttatcgc ttcgagaatt
    43801 ggttatccag ttcttgttcg tccaagctat gtattaggcg gacgtgcgat ggaaatcgtt
    43861 gaaaaccagc gagatttaga agattacatg gaacatgctg tgaaagcatc tccagaacat
    43921 ccagtactag tggaccgtta cttgcttggt ggtgaatgcg aagtggatgc gatttgtgac
    43981 ggtgaaacag tactcatccc agggatcatg gagcatattg agcgtgctgg tgtccattca
    44041 ggggattcaa tggctgtgta tccacctcaa acattatctg aatcgatcaa gaaaacgatc
    44101 gaagattaca cgatccgctt agcattaggt ttgaattgtg tcgggatgat gaatatccag
    44161 tttgtcatcc atgaaaatga ggtttatgtg atcgaagtca atcctcgagc aagtcggact
    44221 gttccattct taagtaaggt gacaggaatt ccaatggcac aaattgcgac gaaagctatt
    44281 ttgggagaga aactaaagac acttggttat gaaaacggac tttatccaga aacaaaagaa
    44341 gttcatgtta aagcacctgt cttttctttt acgaaattgc agaaagttga tacttactta
    44401 gggcctgaaa tgaaatcgac tggagaagta atgggatctg acagacaatt agacaaagct
    44461 ttgtacaagg cattcgaagc ttctggatta cacttgcctg aatatggagc cgtattattt
    44521 acgattgcgg acgaaacaaa agaagaagca ctagaattag ctaaacgttt tacagaaatt
    44581 ggctatagtc tgataggaac gaaacataca gctgcttatt tcgaaaaaaa tggtctggtc
    44641 gttgaaacag tagcgaaaat ttctgaggaa gcagcagaaa aaaatgtagt cgacttaata
    44701 agagatggta aaacacaagt ggttgtcaat acgattgata aagatcgggg aaatgcttct
    44761 aaagacggtt tcatcatccg aagagaagca gttgaacatg gtattccatt gtttacttct
    44821 ttagacacag cagatgcgat cgttcgagtg atggaatccc gtgctttttc aactaaccct
    44881 atttaacggc gagcacatgt taagacaaaa agacgaaaaa ccgttgcagg aggggacaga
    44941 tgaaacaaga aatgatggaa atcgttcagc aaaaacagct ggcaccaaag atttttgaaa
    45001 tgacattgaa aggtgatcta gtaaaagaaa tgcagcaacc aggacagttc ttgcatattc
    45061 tcgtgccgcg tgcagacttg ctattacgcc gaccgattag cttgaatcat atcgaccacg
    45121 aaacaaatac ttgccgtatc atttatcggg tggaaggtga ggggactaga gtcttctcta
    45181 ctctttctgc cggagaccat ttagatgtca tgggtccttt aggcaatggt tttgatttgt
    45241 ccaatcttca tacaggagac gaagtattta taatcggtgg aggaatcggt gtccctcctc
    45301 tatatgagtt atcaaaacag ttagtcgaaa aaggaattaa gccaacgcat tttttaggtt
    45361 ttgccacaca tgaagtcgtt tattatgaaa aagaatttaa tcgtctagga gaaacaagaa
    45421 tcgctactga cgatggaaca tacggtatgc acggaaatgt cggtaattta ttgttaggaa
    45481 cgaagacgga acctacagct gtttttgcat gtggttcaaa cgggttattg aagacagtgg
    45541 aacagctgtt ttcttcgcat caaaatgtcc agttatcatt agaatcccgc atggcatgtg
    45601 ggataggggc ttgctatgct tgcgtttgtc atacgccgga agatgaaaca ggaacaaaaa
    45661 gtgtgaaagt ttgtgacgaa ggaccgattt ttaaaatagg agaggtaatt atatgaatga
    45721 gaatccttta gcggtcaaac ttcctggcct agaattaaaa aatcctatca tgccggcaag
    45781 cggctgtttc ggttttggca aagaatacgg aaaatactat gatttgaatc agcttggatc
    45841 gatcatggtc aaggcaacga ctccaaatgc ccgctttggc aatcccacgc caagagtggc
    45901 ggaaacacca tctggcatgt tgaatgcaat tggtttgcaa aatcctggtc tagacgttgt
    45961 tatgcataaa cttcttccag aattagagga gtacaatgag ttacccatca tcgcaaatgt
    46021 tgctggtgct tgtgaagaag actatgtaga agtttgtagc aaaattggcg aagcaccaaa
    46081 tgtgaaagca atcgagctga acatttcttg cccaaatgtc aaacatggcg gaattgcttt
    46141 tggaacagat tctgatgttg cttttcaatt aacacaagca gtaaagaaag tttcttctgt
    46201 tcctgtctat gtcaaattgt ccccaaatgt aacagacatc gttccaatcg ctcaggcaat
    46261 cgaagctgga ggggcagatg ggtttacgat gatcaatact cttcttggta tgcggattga
    46321 ccttaagaca agaaaaccga ttttagccaa tcaaacaggt ggactctccg gaccagcaat
    46381 caagcctgtc gctattcgtt tgattcacca agtagcaagt atatccagcc ttccaatcat
    46441 tgggatgggc ggcgtgcaga cagtggatga tgttcttgag atgtttatgg ctggagcaag
    46501 tgctgtagct atagggaccg cgaatttcac cgatccgtat atctgtccta aactaattaa
    46561 agagctccct gtgcgaatga gcgaactagg catcgaatcg ctggaaagat taatcaaaga
    46621 agtgagagag gaacgtgaac gatgaatcaa ctagaccaac gaccaattat tgcattagat
    46681 ttttcaacta ggcaagaggt agaagacttt cttcgatttt tcccgaaaga agaaaaattg
    46741 tttgtgaaaa tcggaatgga attgttctac caagaaggac ctgaaatcgt acgttatcta
    46801 aaagaggctg gtcataatgt atttttagat ctgaaacttc acgatattcc gaatacggtc
    46861 gaaaaagcaa tgcggggatt ggcaaagctt ggtgttgatc tcacctgtgt tcatgccgct
    46921 ggcggtattc gtatgatgga ggcggcaatg cgaggattag aagaggggac tccagaaggt
    46981 ggaaaacgtc cgctacttct agctatcact cagcttactt ctacaagtga agaagaaatg
    47041 catgccgatc aactaatcga agtttcttta gaaaaaagtg tgatccatta tgctagctgt
    47101 gccaaaaaag caggattaga tggtgtggtt tcttctgctt gggaagtaga agcgatcaaa
    47161 gaaatggcag gagacgaatt tgtttgctta actccgggta taagaccaga aggaacagtc
    47221 gcaggagatc agacaagagt agtcacacct tctcaggcaa aaaagatcgg ctcaactttt
    47281 atcgttatag gtcgaccaat cactcagtca actaatcctt ataaagcata tcaaacgatt
    47341 caaacagaat ggagctaacc taaaatgaat gttgaacaaa gtatcgcaaa agacttatta
    47401 gaaattgaag cagtattttt aaatccaagt aatcctttta catgggcaag tggaatcaaa
    47461 agcccaattt actgtgataa ccgaatcacg atgagctatc caaaagtccg aaaagagatc
    47521 gcaaaaggat tagcaagcaa aatcaaagaa gcttaccctg aagtacaagt gattgctgga
    47581 actgcaacag caggaatccc tcatgccgca tgggtagcgg aaatactgga tttgccaatg
    47641 gtctatattc gaagtaaggc gaaagatcat gggaaaggta accaaatcga aggacgtata
    47701 tttgaaggtc agaagatggt tgtgatcgag gatttgattt ctacaggcgg aagtgtcttg
    47761 gaagcagctg aagcagcaaa aagagaaggt gcggatatcc ttggtgttgc ggctatcttt
    47821 acgtatgagt tgccaaaagg aaaagcaaat ttcgaaaaag ccgaaatccc tcttatgacg
    47881 ttgaccaatt attctgtatt gattgaagca gctttagaag atcgttatat cgatgagcaa
    47941 gaattaactc tgctaaaaga atggaaaaaa gatcctgaaa attggcagat ttaagcgcgc
    48001 ttgatgaaga tagagaaatc cgatttgtgt ctccagaatc atcaattgga atagcaaaaa
    48061 tccaaaaact gattgcgaga agcatctttt catcaggtat gaaaagaaac ttctatcgtg
    48121 atcagttttt gattttttta aaattcattt ttctaagact ggttagcaga aagaagagaa
    48181 taatctcttc tcttttttct attatatata gattttctct attttttggt aagggagctg
    48241 tttcaaaaat ggtaaagagc ggaaatgttg cgaatatcag ttatttaatg atatgatatt
    48301 cggaaaaaga aatctcaaag ggaggtaact tttatggggt tgaatgaatt tattttagga
    48361 ttgaacaatt tagagacgat cacaagggca cctggatttt ttaaatatac agagcataca
    48421 gttgcagcac attcgtatcg tgtagcatct attgcgcaag ttctaggaga cattgaagaa
    48481 gcgaatggac aaccgatcga ttggaaactg ctctatgaaa aatcattgaa tcatgactac
    48541 acagagcgtt ttattggtga tatcaaaacg ccggtgaaat acgcaagcaa agagttaaga
    48601 gggatgctgc aaacagttga agaaaagatg acggacgaat tcatcaacca agagattcca
    48661 gctgagttcc aagaagttta tcgccgtcgg ttgttcgaag gaaaagatga aaccgtagaa
    48721 ggagaactat tagccatttc agataaggtt gaccttcttt atgaatcttt tgatgaaatc
    48781 atcaaaaata acccggaaaa agtttataag gagatgttca ttgaggcagt gactacgatt
    48841 aaaagctttg aacatcggcc ttctgttcag tatttcttta aagagatatt tccggaacta
    48901 ttgaaccagg aattttatgg aaaagaggcg tttttagaaa ccgtctcaac gattgtaaag
    48961 aaagaagaaa aataaaaatg atatgagatg cagggggaag tatagttgaa aaagaatatg
    49021 gatgtcgaat ggggctattc tggagacaaa ggtccggaaa attgggcgag tttatgtgat
    49081 tggtttgcga gaggtgcgga atttccattg cagtcgccta ttcatttgac caagggggaa
    49141 gaaaccaaaa tagaaggaga cacgctttcc tttcattatc aaaagcaacg gtttactgac
    49201 aaggaattca aaaacacgat tcatttagta ccttttgacc aactcagtta tgtagagttc
    49261 aagaaagaac gctattattt gacagatatt catttccact tgccttctga acatatcatc
    49321 aacggaaaac aggaagatat tgaatttcat tttgttcata tgaacagcaa aaaagagaat
    49381 ctagttgtgg gtgtcatgta ccagctaatg gtacaggaag gttggctgta taatcaagaa
    49441 aatggggaaa gttggaactt agaaaaacat gagcattggg taaatccgga tgtgttcttt
    49501 ccggagcaaa aaagccattt ccattatatt ggctcattga caactccgcc taccagcggg
    49561 ccaatccaat ggtttgtaat ggatcatgta ggaaaactga accaagagtt tcttctcccc
    49621 ttcgatggtc agtatgctcg accgaacaac cgaccaatcc agcccttgaa tggaagagaa
    49681 atttattatt tcgaggagtt tgagcaatga atattcagca gatgaaatac gtggtcgctg
    49741 tagcaaacaa tggtagtttc agagaagcgg caaagaaaat gtttgtctcc cagccaagtc
    49801 tttcagctgg tatcaaagaa ttagaaaccg agttagggat cacgctgttt actcgaacga
    49861 atcgaggggc atatttaaca gaagaagggc aagacttctt aaatcgtgcc gagaaaatat
    49921 tggtccagct tgatacatta gaaactcact atctaacgag tgaaaagaga gacaattttt
    49981 cgatcgctgc ccagcattat gatttcttag gtcctttgac cgcacagcta ctcgaggaat
    50041 tcaaagacga gatcaaacaa tttcggatca tggagacgac aacagcgaga gtgatcgaag
    50101 aagtcaaaga gcagcacagt gagatcggca ttatttatat gaatgagcat aatcaagctg
    50161 gtatcagaag atatttacaa caaggagagc taagtggccg cagcattgga aaattcaaaa
    50221 cgcatatttt tttaaggcaa ggacatccat tagctgaaca cgcagagatc accaaagaag
    50281 aattgttatc ttatcctcaa gttcgtttta cacaggatgg aaataatttc ccgtattttt
    50341 atgaagacct tatcgagatc cctgatcaag aaacagtgat ttatacgagt gatcgaggta
    50401 ctttgatgaa tatcgttctt gaaactgatg cttacgcttc aggatctggt attgtgattg
    50461 gagggattcg cgagcacttg cggttaatcc ctttggcaga aggcgaacta aatgaatttt
    50521 atatcattta ttcggcgaaa cgtccgctca gtgaaatcgc tcaaagattt atcaaagagc
    50581 ttactcggtt actggaccag cagagctaag aaaagcagat gagaacgctg tttattgaac
    50641 accgttgatc aaaaaagagg ggttgtgaca gaattttgtc acaactcctc tttttgtagc
    50701 aaacaattta taactaattg ctgttgtggg aaagtacgca aatatgattc tagaaacact
    50761 cattgattac ctgttttgtt tcatagctgt aattttttct tcttctggtg tcacgataat
    50821 cgtcttttgg ttttcataaa taacataacc aggcttggcg ccatttggtt ttcggatatg
    50881 ctttacctgt acaagatcaa ccggcacttg ggcagagtat cggtattttg aaaaataggc
    50941 agctaactcg gcagcttctg taattgtttc atctgagggc ttgtcacttt taatgatgac
    51001 gtgcgaaccg ggtatatttt ttgcatgaag ccaatagtcg gtttttttag ctgtcttcat
    51061 tgtcagctgg tcattttgaa gattgttttt accaacaaga atgagcgttc cgtcagaaga
    51121 aaagtattga tcaggctgag atggtttctt acgtttttgt ttttttaagt tcttcttttt
    51181 caggtatcct tctgaagtca attcttcttt aattgcttcg atatccattg ggccggcaat
    51241 ttccagttga gataagacag attcaagata ttggatttca tctttggctt cttggatttg
    51301 ttcgccaatc agcttaacag cgttcttcag cttttggtat cgatggaaat atttctgtgc
    51361 attttgattt ggtgtcaatg ccggatctag tgctattttg attggccgat cttcttcata
    51421 ataattaggt aaaacaactt cttttgcgcc gcgtggtact tgtgtcagaa aagtagtaag
    51481 tagttcgcca tttctacggt aattctcagc gttttcggat tctttgagtg tttgttcacg
    51541 ttttttcaat ttatttttat ttcgttttag ttcattttcg atttttcgaa tcagttctcc
    51601 gccttgttgt tttgcccgat ctttctctgc tttttcgtga taatagctat ctaataattg
    51661 actgagtgtt ggataagctg ttacagcaga tgcctgttcg gacaatacct gataagcaat
    51721 cggtgtgaaa aaatctttat tttccgtttc gtaaaaagta ggaataggct gatggctgat
    51781 ggctgagaaa aattcgtccc atacaaccat cttttcatta ggacgttcca gtaatctttt
    51841 ggtcagctct tgagcgatat cgaaaccgat tccctgaaac tgacgttgga ttgcttttgg
    51901 atctagttca gtttgggaaa gccgctgaaa gattttttct ttctcggagc taaatgggtt
    51961 caagacctct tgttttggcg gagcaacata ttctacacca ggaagcaaag aacgataagt
    52021 gttttgagag cttccaatgt gtttgatcgc atccagtatt tttccagttt ctcgattaac
    52081 taagacgatc gtactatgac gtcccattaa ttcaacgatc aatatgatat tttgtagatc
    52141 acccaactcg tttcgtttag caaagtggaa atggatcact cgatcatttt cgatttgttc
    52201 gatgtcttct aaaatagcgc catccaaata ttttcgaagc atcatcacga agttaggcgg
    52261 attatcagga ttctgataat cgatttgggt aatctgtaca cgagcataac ttgggtgcgc
    52321 agataataat aatcgatggt ttttgccgcg tgaacgaatc accagcacga tttcattttc
    52381 ataaggctga tggattttcg agatccggcc agatagtaac gtttcacgca attcgttgac
    52441 catcgcgtgg gtaaatacac catcaaagga catggttcat tcctcatttc ttttcataag
    52501 tatattcatt ataacgaaaa aaagaagaaa aaccaatttg gccgaatagt aaagcaaaag
    52561 tctagaaaaa tcttggacta attacataaa aaacagtgtt tctatcgttg aaatccaaat
    52621 ttttaggtat aataaaaaat aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa
    52681 gtagttaaat cgagagtaca agaaatgaat gaacatttct cagtgtcatt cgcagaaaat
    52741 ccaagtaagt attttcgacg tcttgttttt ctgctcatct tatcattgct gttaacagtt
    52801 gcaatgtttt tattgccgga aaaaatccaa gaagagcaag taaatagtct cgtttattcg
    52861 tccataccaa ccagcaaatt gaaacctttt gattacagca aagctgatca gaaaatcaaa
    52921 caaagccggg cgatatcggt gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg
    52981 cttgatgttt ttaatgatcc tgagcttatg gaagaattga atcgaccgat cttttattat
    53041 ccgatcgttt atgatgtaca tgaactggaa caaaaatata acatacgaac ggatgaagtg
    53101 acatttattt tctttgatgg aggaaaagaa gcaaatcgga taactgcggg taagggagga
    53161 ataactgatt ttgataagga gttgatccct gaactgaatc gacttccgtt agccaatatc
    53221 aaacaattag aagaagaatt ggatagaacg ggagtaactt cccaaaattc cgagattgct
    53281 aattgagaca ttggcgcgat gaaacggtgg gtctatatag cgctattttt aattatcaga
    53341 acaagtgatg tttgatcttc aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa
    53401 ttcaaaaatt cgattagact taaatcaatt cttaaggaaa gggtgacctg taatgaactt
    53461 atcaaaacgt atttcagcag aaaacaaaaa aaattggcgc agctggcgta gataagttgt
    53521 attaatggtg agactttccc atagatacaa ctttttagag tgcatttcta aagcttgtat
    53581 ctatgctaga catgaacatt gactgccgca tagattagaa tctatgcggt tttttgtgta
    53641 aagacaatga ccgctttggc cattgtcttt ttttgtagac tggatacttg cctgattata
    53701 ataaataaaa agattgactt tgcaggagga aaaaaatgaa aaacaaacga ttaataacag
    53761 ttgtagtaat tattttagtt tatttagcag gtgcatttat ttatggaaaa gcctcaggcg
    53821 aaaaagaaag tacttcgacg aaccagacaa aaaaaacaac agttgttggg gtattgcaat
    53881 acgtgagcca tccggcatta gatgagattt atcgaggaat caaagatggt ctgaaagaag
    53941 aaggattaaa agaaggagaa aatcttgaga tcaaatttca aaatggacaa gccgatcaga
    54001 gcaagctagc cacaatgagt caacagctag tacaatctga tccagatgcc ttgattggaa
    54061 tcgcaacacc tgccgctcaa tcattagcaa acacgacttc atcgatccca gttgtgttag
    54121 gtgctgtaac ggatcctgtg ggggctggat tagtcgcttc tttagagaaa cctggtggta
    54181 atgtaacagg tgtttctgat cagccgcctg ttacttcaca aatcaagctt gcttctgatt
    54241 tgttgccaaa tgcaaaaaaa gtcggaatct tgtatgcttc ttcagaggat aattcaaaat
    54301 accaagtagc acaagcagaa gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg
    54361 cggtgccttc cactaatgag attgcacaaa cggttcaagt gatgagccga caagtcgatt
    54421 ttatctatgt tccattagat aataccattg ccaatgcgat gcaaacagtg gtaaaagaag
    54481 caaacaaagc aaatatcccc gtcattccat ctgtggatac aatggtggaa caaggtggac
    54541 tagcaacaat cgggatcaat caatatcaat tagggcttca aagcggtaag atggcggcag
    54601 aacttgcgag tggaaaagaa aaaccggaaa cgacgcctgt ttatacattt gatcaagggg
    54661 atacagtgat caatcaatct caagctgatc gcttaggaat cattgttccc caatcgatga
    54721 aggaaaaagc caaaatcatt acagatgact cgcagcaaga aacatctaag gaggatgaca
    54781 aatgatcgta tcagctattt cacaaggcat gctttgggca atattaggat tagggatttt
    54841 catgacttat cggatcttag actttcctga tatgacgaca gaagggtcct tcccattagg
    54901 tgctgctgtc tgtgtgacag ctattactca tggggtagcg ccgattgtag ctacattatt
    54961 aggcgtaggt gctggcatga tagcaggact cgttacgggg ttactttata caaaaggaaa
    55021 gataccggtt attttagcag ggattttagt gatgtcagga ttgaactcag ttattttgtt
    55081 tgtgatgcga acaccaaatc tttctttatt gaatcatccg attctacaag acagcttcca
    55141 tcgctttggg ttacctgatt actttgacac agtccttctt ggcttcatct ctttgggcat
    55201 cttgattgct gtactgctat tcttcttcaa tacagatttg ggtcaaggct atattgcgac
    55261 aggagacaac gaaacgatgg ctcggtcgtt agggataaaa accgatcgta tgaaaatttt
    55321 aggtttaact ttgtcgaacg gtgtcattgc attatccggt gcgctgattg cccagaacga
    55381 cggatatgca gatgtaaata aagggattgg cgtaatcgtc atcggtcttg cttccatcat
    55441 catcggtgaa gtgatcttcc aagaattgac gctagcagaa agattgattg ctattgttgt
    55501 cggcagtatc atttatcagc tattgatttt agtagtaatc aaactagggt ttgacacgac
    55561 ttatctgaag ttgttctccg ctatcatctt agctatttgt ctgatgatcc cgcaattgaa
    55621 gaaagcactg aacttaaaaa cgggttttga gaaggaggca tagtaatgac tgatatattg
    55681 acaatcaaaa atggcagaaa agtcgtaaac aatggaatga atgaagagaa agtactattg
    55741 gatgaaatta accttacaat cagagaaggg gacttcatca ctgtccttgg cgggaatggt
    55801 gctggaaaaa gcacactgtt caatacgatc gctggtacgc ttcaactcag tcaaggatcg
    55861 atctctttta aagatcagct gatcacaaaa gattcagaag aaaaaagagc acgattcctt
    55921 tctcgagttt tccaagaccc caagatggga acggctcctc ggatgacagt agcagaaaat
    55981 ttgctacttg cacaaaaaag aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat
    56041 caaaaagaag attttttcag actatgtcaa gaagtcggta atggcctaga gcaacacttg
    56101 gatactccag cgggtgaatt atctggtggt caacgccaag cattaagctt gctgatggcg
    56161 acgatcacag ctcctgagtt actattatta gatgaacata ctgcagcttt agatcctaaa
    56221 acagctaaag cattaatgcg attaacaaat cagcggatca atgaacaaaa attgacttgt
    56281 ctcatgatca cgcatcgaat ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc
    56341 caaaaaggga aaatcgttca agatatttct gaaaaagaaa agcaacattt gacaatgggc
    56401 gaccttttgc agttctttga agaagtagtg gattgatttc gacagaaaaa ttcctttcag
    56461 tagaaggact tgaaaactca ttgacatctt cctttgattc atcctatact taaaacaata
    56521 aacaattaaa catatttggg tgttttgtag gatcacgtag tcaatgattt taacaattaa
    56581 atagaatatt ctttgctctg acaagtaaac gatgagaaaa gaatttgcta ctttgccacc
    56641 tagacgtttc gtactttttc gtatcaaacg ctaagtggct ttttttattg aagtaagaac
    56701 aaaggaggaa taagatgatt tctggacaaa ccaaattagc tgggctcttt gctgatccgg
    56761 cttctcacag tttatcccca ttgatgcaca atactgcttt tgaagcaaac aacatcgatg
    56821 cggtgtattt agctttccaa gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc
    56881 gaacttttga tatgttaggt gtaaatcttt cgatgccgaa taaaacagcg gttattcctt
    56941 atctggatga ggtaagccag gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg
    57001 aaaagggtcg tttgattggc tataatactg atggtgcggg cttcatgcaa tccgtgcaag
    57061 aagctgggat ttcgatcaaa aagaaaaaaa tgactctact gggagctgga ggtgcagcaa
    57121 aggcagtcac tgttcaagca gcgttagacg gcgctgagga aatcgtagtt tataaacgaa
    57181 acaaccaaac attcgctcaa gtaaaagaaa cattcgaaca actcagctat cgaacttctt
    57241 gtaagataaa agttcgtgac tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa
    57301 gctgtcttct agtcaatgca acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc
    57361 tcttagatat aagcatgatg catgagaatc tagcggtttt tgatttgatt tatgcaccta
    57421 gagaaacacg tttattaaaa gaagcaaaaa aagtaggagc aaaaggatac aatggacttg
    57481 gcatgttgat ctaccaagga gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga
    57541 tccatgtgat tgaaaagctg tttgattaaa aaaggataat agataaagat cttttattct
    57601 cgcataaacg gtctcaagaa aagtaatcct ttatgtatcg aagcatttat ttcgttattt
    57661 tttattaaat tatcaaaaaa tgataggctt ttgtcgattg gttatcgtat cctttattta
    57721 tcagtaagaa acgaggagga accacaatga gtcggatgga tcgctataag gacatccata
    57781 agaaggcgaa acctctgaag cgggatacgg ggagtatttt tgctagaaga gaacgaaaac
    57841 gaacgcgtat ggaaaatgag gaagatgcta cccgtattta tgaaagagag cagcattcat
    57901 ttgaacattt gaaccaatac gagcaacaga tatacagaga tgcaccaaga gcagaagaac
    57961 aacaaaaaaa taaaaaattt ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa
    58021 gaaagcgatc gtggaaaaaa atcattctat cgattttgct tgttttgatc ctttattcag
    58081 gtatctcatt ttttattggt aaatatgtag ctgaacatga cacaagctta gaggcagcga
    58141 cttcagaaac ctttcatggg caaaaagcga cgaatggtgc gcataatatt ttgatccttg
    58201 gaagtgatac acgcggagaa gatgccgggc gagccgatac gatcatggtc ttacagttgg
    58261 atggtccttc ccataagccg aaactgattt cttttatgcg tgacagcttc gttacgatcc
    58321 caggggtagg acaaaataag atcaattcag cttatgcata cggaggagcc gatctagtgc
    58381 gtcaaacttt agtagaaaat tttggaatcg attgtcaata ctatgcgaaa gtcgatttca
    58441 aatcgtttga aaaagtgatc gatgccttat tcatgaacgg agtgaagatc gatgcagaaa
    58501 aagatctgaa tctggatggt gtagatatta aaaaaggggg ccagaagatg gatggacatg
    58561 ttcttttgca gtacgcacgt ttccgaatgg atgaacaagg agactttggc cgtgtcagac
    58621 gccaacagca agtaatgaac gctattttca gccaattgaa gaatccattg aatctgatcc
    58681 gtgcacctta tgcagctggt aaagctttag gatatacatc cacagatgtc actgcttggt
    58741 tcttgacgaa aaacctcttt tcgattgcac gaggagctgg tggcattgat cgaataagtg
    58801 tgccagtaga cggttcgtgg aattatgggc aaagtgatta tgctggcagt attcttgaaa
    58861 ttgataatca aatgaatcaa tctgctatta cagatttttt gaataaatga tcataaaaaa
    58921 gatcaagaag aagctgcgca aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa
    58981 aaacgctttc ttatggtaga tttaaccaag agaggagctg tttacattgg atatctatct
    59041 gaacttcaaa aatgagtcgc gtgaagccat cgctttttat gaaaaagttt ttcagactga
    59101 agcatcaggt atcaccacat acggtgaaat gcctggaata gaacaaatga acttgccaga
    59161 agaagcaaaa aatctaatca cgaatgcacg gctgaacatt tatgatacat tagttatgtt
    59221 tgcagatacg ccagaaggaa ttggtcctga attagttgtg ggaaataatg tttctttagt
    59281 catccagaca agaaatgaag aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg
    59341 taagattgag atgccactgg atgctgttcc gtgggctcag ttgtatggga tggtaactga
    59401 tcgatttggc atcaactggc agattaacca agagtgacag ctaaagatga atattttctg
    59461 gaaacatcaa gcagcaaacg gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg
    59521 actatacggt atcttttttt tattgccaga tgcgaaaata tgttccataa tagagaagtc
    59581 cgcaaaaaat taagtaacgg aggtcagaag atgaagacgg tgatactagc tgaaaaacct
    59641 tcacaagcaa aagcctatgc cgaaaccttc caacagcaaa agcggcatga agggtattat
    59701 gaaatcagcg atccattgtt tgctggtgaa gtaaccatca cttatggttt tggacatttg
    59761 gtagatatgg ttgctccagg agcttatgaa gagcgttggg caaaatggtc tttagaaaat
    59821 ttgcctattt tcccagaaac cttccgttac gaagttccaa tggacaaacg tgcacaattt
    59881 tcgatcgtaa aacgagaatt gcagtcagcc gataccatca ttattgcaac agatggtgat
    59941 cgagaaggtg aagccatcgc gtggtcgatt attcgacaag caaacgcatt ttctaaggaa
    60001 aagaactatt tacgtttatg gatcaattct cttgaaaaag aagcaatcta tgaaggtttt
    60061 aagaatctcc aacctggaaa gaactatttt ccaaaatata aagaagcgca agcaaggcag
    60121 aacgctgatt ggctgattgg aatgaatgga agtccattat atagcttgct acttcaacga
    60181 aaagggattg atggcagttt ttctcttggt cgtgtacaaa caccgacact ctatatgatt
    60241 tatcagctgc aagaagaaat caaacacttc aaaaaagaaa cgtactttga aggagaaggt
    60301 ttaattacga caaacaaagg gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa
    60361 acccaagaag agttaacgaa ggaaatagaa aaacttggag cgcatttagg taagcaaaac
    60421 ggacggattc ttgaagttac gaaaaaggaa aagaggttgc atagtccacg tttattttcg
    60481 ctatctagtt tgcagacaaa gatgaatcag ctaatgaaag caagtgccaa agctacactt
    60541 gatgctgctc aaggattgta cgaaagcaag tttctaagct atccaagaac ggattccttt
    60601 tacattacag aaaatgagca tcagtatctg gttaaacatc tgcaaaatta taaaaaattt
    60661 ttaggaatag aagaagtaca gacaactgag atcaaagcaa agaaacgata tgtcgatgcg
    60721 aaaaaagttc aagaacatca tgcaattatt ccgacaaaga cgattccgac tcctgcacgc
    60781 tttgcgaagc tttccaaatt acagcaagca atctatttgc aagttttgcg cactactgtg
    60841 gctatgtttg ctgcagatta tatctacgaa gagacgaccg taatgacggg cgtacagcaa
    60901 ttaaagctaa aaagcatcgg aaagatccca ttacagcaag gttggcagga aatcttacgc
    60961 atcaagtcaa aaaagaaaga agctcagact cttcctccta tcgtagaaaa tgaagaagca
    61021 gaagtagaac tgaaaagtat ggagaaagaa acacaacctc caaaacccta taccgaagga
    61081 acgttgatca cagcaatgaa aactgcggga aaaacgctgg ataatgatga agcgcaagaa
    61141 attctcaaag aagtagaagg aatagggaca gaagctacac gagccaatat cattgaaaat
    61201 cttaagcaga agcattacat cgaagtcaat aaaaacgaaa taactgttac gccaaaagga
    61261 atcactcttt gtaaggcagt agccaatgaa ccattactga cgagcgcaga aatgactgcg
    61321 cgctgggaag ggtacttgag aaaaattggt aaacaagagg ggacaccatc tgttttttta
    61381 gagaatatca aaaaattcat cctacattta ttggcagaag ttcctaatca aatcgacaag
    61441 gtagactttt cagatgagat tagcgggaca cgtcagataa aagcgatgga gaagaaaaac
    61501 gatcaattag gaagatgtcc aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc
    61561 gctacatgct tgaatccgga atgcgacttc aaattgtggc caacagtagc taaaaagaag
    61621 ctgactaaaa cgaatctcag agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa
    61681 ggttttactg gtaaaaaagg gaaattcgat gcagttttag tattaaaaga agatatgaca
    61741 atcggttttt cttttccatg gatcgaaaac agtggaacaa aagcagttga tggaaacaaa
    61801 gaaacatcaa gaaaaaataa tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg
    61861 aatatttatt ccgaaaagtg agaatataaa taccaataaa actaaaaaat atcagtgaat
    61921 caagcaaaaa cgttggggta ggctgatttt atatagatag caaggagttt ttttatttga
    61981 aaaaagtgca ttcattttta aattatctta tgggagcaat cacgattgtg gctgtgtacc
    62041 aagcatttct aaagtatcct gattacggga cgaagttgtt gtcattgacg cacaatcaaa
    62101 cagcatattc accaacgttg attttatcga ctttgactgc aggagctaaa agcgctactt
    62161 atttttttca taaaacgaca agcatgctaa agcgaaagaa acagcgaaaa caattgttgg
    62221 tggtggtagc ggctatgatc attatttatt ttctaccagt tattacacaa gtatttcatc
    62281 aatattttgg ttaagaatac gagagtttgt gacaaaagtc atgtcacaaa cttttttctt
    62341 tgtcattttt tcgtaagaaa aagcaaaata aaagtatata cattgattaa aagtatatac
    62401 ttggatataa tgtacttact atagaatgag aatggtatag agggtggcga aatatgaaaa
    62461 aaatgaaaaa gtataaaatt agaaaatctg gcaatagcga tgtaacaact atcccaccag
    62521 aagtaaaaga attcatgggt gtgcagacag gtgacgcaat cagttatgtt tttcaatctg
    62581 atggttctgt acgaatgatc aaagcacaag aagaacctga tattgattca ttagttgatt
    62641 cgatcatgaa tcaatatgaa gacgcgttaa aagatttggt tgatttatga ggtatctttc
    62701 gactaaagaa attatcaaaa tcaacgcgaa agttatttta cgtcactcgc caggcgaaat
    62761 gatcggtgta aaagatgcaa atgcattaga tatggccgtc aaacagcctt cacaagcagt
    62821 attcaatcaa gaactctatc ctgaaatcta tgaaaaagcg gcgattttag caattaattt
    62881 agctaaaaaa catccttttc ataatggcaa taaacgaact gctcttgttg ccatgttgtt
    62941 gtttcttcaa ttgaataatt gtccagttgc tttttctcgt caagaagctg ttgattttat
    63001 cataatgatt accactagtt cattggattt cgattcacta aagtccaaga taaccagtta
    63061 tttacgacaa accgcaataa aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc
    63121 atgtcattaa ggaatatgct gattttttta tgtttaatca tacaaaatag ttttttcata
    63181 aaaatatgga aaatatggat taattttgaa ataattgatt taaaaggaat acaaaagagt
    63241 gaatagtagg aaatagtggt ttagaactag tgggctgatt ttttatatat tttagtttac
    63301 ataatatata ttatacaaag tagatttaaa aaatgataaa cctctctcga acggtttttt
    63361 atcaaaaatc gtcatttttc catcaaaatc tgcttatgca aaattataaa aaaattttgc
    63421 agtttttctt actttttttg tcgtttaatt gaatgataaa aaccagtttc tttattcagt
    63481 taatcaaata tgaacgaatt agatatgaat cattgtattt gccgttgcgg aaggattttt
    63541 taattaacca cagataaaca ttgataacga ttcgcttatt gtaatcaaca gattaaattt
    63601 catatgttca tatgtacgga tacattttcg gaccgattaa ttttaaccat tctgtttatt
    63661 tgcttaccta ttagatatca ataaaaaact gcttaacagc tatttcagct gttaagcagt
    63721 tttaggattt ttagttcata ttcattgttt cttataatac agtgttcaat acttctctta
    63781 aagcacttgt tgatttgctg aacattgctt tttcttcttc gttgattgat aattcaacaa
    63841 cttcacgaac accattttcg tcaacgattg atggcacacc tgtaaagata tctttttcgt
    63901 cgtattcacc agttaaataa gcagatacag gcaatacagc ttgttcgtta ttcaagatag
    63961 cttttacgat acgtgcagta ctcataccaa taccatagta agttgctttt ttacgatcaa
    64021 tgatctcata agcagcatta cggactttat cagcaatcac atctaattca tcttgggcaa
    64081 tacgatggtc tttttctaca atttcaaaca ctggtttacc accgacagtt gtatgtgacc
    64141 atgcagcaac ttctgaatca ccatgttcac ctaagatata gccgtggacg ctgcgtgggt
    64201 caacttttaa tttcaatgcg atttctttac ggaaacgagt tgtatccaat gttgttccag
    64261 taccgataac acgtgatgtt ggcagaccag attcgttcca agcgatatat gtcaaaatat
    64321 ctactgggtt tgaagcaaca acgataatac cgtcgaatcc tgatcccatg atttctttta
    64381 cgatttggcg catgattgac gcatttgttt taactaaatc taaacgagat tgaccaggtt
    64441 tttggttgat accagctgtg ataacaacga tatcagcatc tttacattct tcatagccac
    64501 ctgaccatac agccacattt tcatcgcccc aagccatacc atctaaaagg tccaatgctt
    64561 ccccttctgc tttttcttga ttcacatcga tcaatactaa ttcattagag attccttggt
    64621 tgatcattgc ataagcgata cttgttccaa caaaacctgt accgacgatt actactttac
    64681 gacttgtttt tttcatgaat aaacagctcc ttttcataac tatttgttac tttcatgaaa
    64741 agtatagtct tgtttattca ttttatcaac atgttaatta taatagttgt tattataatt
    64801 tttgatggaa acgaattcct gaatacgcat aacagataaa tgacaaatgt ttacacctat
    64861 atcccctgat aatacaacca aaagtttaga actgaaaata aatccaaaaa aattcgctgc
    64921 aacgatataa taagacagca atcgcaccta aaacaatttt ccttcatatg ctttagtgaa
    64981 agattttctt cttaatgttt atttctccat tcattgggag tacatgttga atacttcttg
    65041 aataatttga ataaagaagt cgtttgttta tatccgatac gattagcgat ttcttccaac
    65101 gaaaaactcg tttcttgaat cagttccaac gcaaagtcga attttttctt tagaaccagt
    65161 tcttgaaagg aagcatcaaa atacattttc aatttattac taagatagac cggattgtag
    65221 ccaaaatgag ctgctgtttc ttttaacgta caagaagtaa aatgcttatc gatataatct
    65281 aatactctaa gcatctcatt ttctgcgcat ccatctgctg atgaaaagct ttgttgttct
    65341 aataaatgag tcaattcgat cagtaatagt gatgtataga gatttaacga acggatctta
    65401 tgactgtatg tatcaaaata aatcaacagc atattttcaa tcaaatgcgt caaacgactt
    65461 gtttttccgc cgcgaaaatg aatgaattgt gtatgttccc ctttttcttt tgatgcattt
    65521 atcaaaaaag aagaaagtag attatttgtg cttgccaaat gattcaatat ctctgtagat
    65581 aaggtttctt cttttaataa gatgttgatt aaaatatcct cttgccctgt gtattcgatt
    65641 ctttgagtag cttctttatc aatgatcacc acgtcacttt cttccatctc tatcctttct
    65701 ccattaataa tttgtaaaca tgtaccgcta tacatataat tgatttcaac aaaatcatgt
    65761 gtatgctcgg gaacttccgc atatcgacgg tgtttactga tatgaatatt tccctgttga
    65821 aatagatccc ctcggtggat ttttggcggt gtctgaagat ggtaagctaa gtttaaatca
    65881 ttgatattca ttccttgttc acgctgcaga gtttcgattg attcttcgtt acgcaaaaaa
    65941 aatgtctaat tcagctagtt ccaattgact ccctcctttt tgtaagcggt ttctttcgct
    66001 tattagttca cgctgtactg ctattatatg caaagctatt tttggtttca taaagacttt
    66061 ctatggttgg gacatgaact tcctgtgatt ccgctattga gagaataacc acttcatact
    66121 atatttttga taagcgctta caaaaatgaa aggaacatgc ttatgaataa aaagatgatg
    66181 aaattttctc tgctgtcagt ctctcttatt ttgacttctg ctggtgcagt cacgtctgca
    66241 attcccagta tgtcagctac ttatgccacg cgttctttat ctgctgttga attattaacg
    66301 actgctccct cttttatggt cactattttt gttttgatta gtagttttat tgctaggaaa
    66361 atcggacaaa aacaaactgt gatcatcgga ttagtgattg catcgattta tggtatcctc
    66421 cccctcttct cacaaagtta cagtattgtc ttagcttcaa gagctggact aggtattggc
    66481 tttggattga tcaattcttt ggcagtcagc atgattgctg ccttttatca cggagatgaa
    66541 cgagcctcta tgattggttt tcaaagtgca tttcaaggat ttggtgcagc tttaatgaca
    66601 tttgttgcag gacagctagt acagttcggt tggcaatata cctttttagt ctacgccatc
    66661 agcttaccta ttcttgtctt gtttgttttg tttgttccta cacctgaaac aactgaactt
    66721 caatcacatg gcacagcccc aacaagacaa gtaatcaaaa cgaatcctaa aatgattagt
    66781 tatggtattt ttctcttgct ggtcatcgtg gtatataatg ctgtatcgat taaattagcc
    66841 acagtcctga cgacttattc tctaggaaac gaaactaatg cagcgaatat cttaacaatc
    66901 atgcaattgg caagtatggc tgccggagtt tgttttggct ggcttcagac aaaagcacat
    66961 cgctatatgc ttacactttc tttactactg atgggaatcg gctttgtatt gattgccact
    67021 gtgataaacc tgtatgtgat tgggtttggt gcgatcctaa caggaatttc tttttcgtta
    67081 ttcattcctt acttattcaa ccaagtaagt atccaagcgc ccaagtacgc ggagagtttc
    67141 aatacttcaa tcttattggt tggcgctaat ttaggtactt tcattgcacc atatggtttg
    67201 ctctttctct cttttgcttc tcttgggagt aaaacacttg ctgttttcgt caatggcagt
    67261 atcttgctgt tgattagcgc gcttattagc cttctagtag tacacaggac gaatagaaag
    67321 acagtgataa gagtagatga agaaataaaa gaacaattga caacaaaata aatttgatgg
    67381 aggtcgtgta aaatgacaaa tgaaaaacat aaagatagat ggctttggta tcctggagac
    67441 tttgagatcc gccatggact gctacaaaat ttccaacggg aagaacgagg ttttgactgg
    67501 ccagcgtact ggtacatgga tgattgccat cggaacgtca agtttaagag atattacttt
    67561 cttgatcaac cttccatgtt taaagtgacg atccaaggag ttgggtatgt agaaattaat
    67621 ggacaaaaac atccatgtgg aaaatggctg acttgtccag ctggtaaagc aaaaattcgt
    67681 atctttgtag gacatacatc agggctacca gcgatgttta tcgagggaga cgaagtaaaa
    67741 tcagatattg gctggacagc aagtaatttt attgaagaat atccagctgg ctggagtccg
    67801 ctttatgtcg atatcgcaaa agatccaaat caaatctatt atcaaaaaga atgcgtccat
    67861 ccagtcactg aaacggagat tggcggagga atcttattcg attttgggcg agcagtgaat
    67921 ggctgtgtag aactaatatc tctggatgac catatcgaat ctatcactct atgttatgga
    67981 gagtccgatg tagaagctat ggatatcgat tattgctact ataagcaaat aggaatgaca
    68041 agaaaaatga aagctcgtaa acgcgctttt cgctatttgt ttataccaga ggtttctact
    68101 ggaatggttc aacttcaagt ttttcatgaa tttttatctc tttcgaaaca agctgctttt
    68161 tcaagcgatt cttctttact gaatcagatc tggtcagtat cagaagaaac cttcaagtta
    68221 tgtagtgatt tgttttttat cgacggtatc aaacgagatc gctggatttg gtctggagat
    68281 gcgtatcaat cattcatgat caatcaatat cttttttttg acgaggaaat caataaacga
    68341 acgatattag cattaagagg acaaaatgaa atcaagcagc atatgaatac tatcgttgac
    68401 tattcgatct tgtggttgat tggtatcgaa aaccactata tgatgacgaa agactatgaa
    68461 tttttacgca tcgtctaccc taagatgcaa tcattaatga gatacttgat ggagcaaacg
    68521 aatgaattag ggtttattta tgggagagca aaagattgga tatttattga ttggtctgaa
    68581 atggataaag aaggaacgct tgctgcagaa caagtcttat tactgaaagc ctatcattcc
    68641 atgatcactt gcggagatat cttagctgaa acatatggag agaacgaaaa ttatcatact
    68701 catctctatc aagaaaaata tcaactattg aaacaaaacc tatttgacta cttttgggat
    68761 gaagaacttg gcgcttttat cgattgctat gaatctggga gaagaaatgt gacgcgtcat
    68821 gcgaatattt tcgctgtttt attcgatttt gctaacaaag accaacagca atcgatctta
    68881 aagaatgtct tattgaatga cactatcaca cagattacta ctccatattt caaattctat
    68941 gaacaagatg ctttatgcaa actcggacag acgaatatcg tctaccaaac gattcttgat
    69001 tattggggag ggatgttatc tcatgatgcg gtaactttct gggaagaata cgaccctact
    69061 caaacaggaa atgaaaagta cagtatgtat ggagatcctt atggaaaaag cttatgccat
    69121 gcatggggag caagcccaat ctatctttta ggacgctatt ttatcggttt gtatccaaca
    69181 aagccaggtt atgaagaatt tatgatacaa cctaatctag attcatttga aacactgaat
    69241 tgtacgctac ctatcaaaga tggaaagatt tcgcttgaaa aaacaaaaga taagatcgtg
    69301 attacttctg atagaacagg cggaacagtg aaaatgaatg gtaaaagtta tcctcttcta
    69361 gcaaaacagc cacttgtctt aacaagctat tcaaaaataa atgcataaaa caaaaaactg
    69421 cttgcgacat atcagaagac tttaaatctt cttctgatat tgtccaagca atttttattt
    69481 tgaagtttgc ttaattctgt tctttagaaa caatgtttgt tctttttccg gcaaaaagaa
    69541 agctagataa tacgacaagt aaaaagaaag cgatgattcc aactgtcaag gctatttgac
    69601 cgtattctcc tagtttccca agtacaagtc ctggtactag ataatcagta tcggcaaatg
    69661 tagaagaagc gattttaaga tctcctaata ctggaaggat aaataatggc agccaactaa
    69721 tcaataagct attgaaagca gaacctaata ccgcccctct tctaccgcca gtcgagttgc
    69781 cgaagacacc agaagcagct ccgcagaaga aatgtcctac taccccagga atgataactg
    69841 tagtttgcaa gcctatcata gcaaacatgg atagtgttcc tacaaagaag ctaacaaaaa
    69901 agccaattaa tacagcatta ggtgcatatg gaaaaacgat aggaacatct aatgctggtt
    69961 tagaatcggg tacgagtttt ttagcaatcc cttggaatgc tggaacgatt tctgccaaca
    70021 ccattcgaac cccttgaagg acgataacaa acccagctgt gaatgttcca gcttggatca
    70081 gtgcaaagat tacagcgttg tctccattgc ttagttctct ttcaacaaat ccaggaccag
    70141 ctaacaaagc aagtgcgaca tatacgatcg acatcaagag catgatcgat acagtagaat
    70201 ctcgcaaaaa gctgaaactt ttaggaatct ttatgtcctc tgtagaacgg tctttgtttc
    70261 caaaaaactt tccgatccat ccactagaag cataaccgat atttccggta tgccctaacg
    70321 caacttgatt gtttccagtg atgttcttca taaacggttg acaaagagca ggcgtaatag
    70381 ttaatagtgt tccttcgaag accccgccta ataagatcat ccacaaattt gcagtaaacc
    70441 ccgcgcttac taaaatgact gctgttagac aagaaacata aagcattgct tgacctgtaa
    70501 gaaaaatgaa tttatatctt gtgaatctag caagtaaaat attcacgatc atccctacta
    70561 gcatgatcaa agcagtaggt gtaccgtatt gttgcaaagc aatcgccact actgcttcat
    70621 tgctaggaac tactccttga gtatgcagag ctgtttggaa catcgaagca aaaggggtta
    70681 gtgaattact gattaaacca gcaccgccga tcagtactag gaatccgaca aatgttttga
    70741 tagttccttg gatcacttct gaaaatttct tcttctgaag aacgagacca agcagtgcaa
    70801 tcaaagaaac gaggatggag ggctgactga cgattgacac aaacaaattc aacaatttat
    70861 ccattgatag ttcctccctt gaatcatttt tattcttttg acaagatctt cgttactttt
    70921 tcttttactt cttcgctgtc aatgatcgat tgcaaaggta cgagcttttc atcaggtata
    70981 gaagttagcg tatctttcaa agaatgctca atgaagaaat aatcagccat atcgctagat
    71041 acacttccaa catccgtatg acttacttca aactcgtcca catctgctcc aagatctgca
    71101 agtgcttgtt tgatgttcat ctctaccata aaacttgttc ctaatccact ttgacatact
    71161 gcaaaaattt tcatatcatt cgtctccttt tcttatgatc gctaatattt cttcttttgt
    71221 tgatgcgtgt aataaatctt gaagattttg tttattagaa agtagctttg ttaaatttgc
    71281 caaagcccga agatgtgcct cattatcgat agcagctaaa cagatgaaca aactaatggg
    71341 atgggctgga ttatcaagca gatccacagg ttgttgaagt ttcagcaaag acatccctac
    71401 ttcgtttacg ccatcttctg gacgagcatg aggtaatgcg atgtgatcgc caatatggat
    71461 aaacgcacca tactgctcca cgcgctcgat catagctttg atataacttt cttctatttt
    71521 attttctgat aatagcggct gtgcggcaaa tgtgattgct tcttgccagt tttttggttg
    71581 atccgataat tggatcattt cttctgttaa taattcagat agcataggac gttgatcctc
    71641 cttcttttct aaaagtttgt tcattttacg atttaatact tgatagagct tttcttcgtc
    71701 tacgccttct tttaattcga tataaggcag tagcgccgac aacaattctt gggcagaagg
    71761 cattgaaaat cctggtatca gccaatctct ttgtacttga ttgatcagct gatttttttc
    71821 caagtcagtc atgattggat gcagaacata gagagacttt ttcttatcag taagtgtgag
    71881 aggtacagta gaaaaaataa tatcgtaaga agtttcgtca acctcgtgta agcggtcagc
    71941 tgaattagtc tctgtaaaaa agatcatcgg gaataattgc tgaagttcac tttttaagat
    72001 caatgaagag ctaatgccac ttggacagac gatcacggct cttatttttc tatttctttc
    72061 ttctgcgtcc gcttttctga tctctccgcc aaatagtata gtgaaaaaac caatctcttc
    72121 ttcagggatg ctcttcccta ttcgtctttc taacggaagt aatgcttttc ttgtcatttc
    72181 gtagatggaa gcatattgat gcttaatttg atcgatcatc acattaggta aataaaaaga
    72241 ataggaaata cgaaaatagg cagggaccaa gtgataaaag acattcatca ataattcacg
    72301 aaacgaagca aattctacgg ccatcaggaa ttccatgcga tggatgactt ctgaagaaca
    72361 ttccagcaaa aaatcaagtg caggctcctg gacatctcct tcaacgatcg atgctaagca
    72421 aagagagaaa aatataatct ccgaattttg atgttcggaa tagccaaggc cagtcaatac
    72481 ttgtcgacta atgtcagtga ctgcaagaga agccaacagt tcagcatctt ttaccagttc
    72541 catggatgtt tttccaattc gaatggttgt caaactcaaa taataagcta attcatccat
    72601 tcgatttggt gcgaatcgaa gatctgatag tgaaatcgtt ttatctagca aatctctaac
    72661 tttggcgtaa taatcaatgg aaagggttgc ggttaaatac gtgagcccaa atcttccagc
    72721 aagtgactgc atcaaacgag cagtgcgatt ttttgcttct cttcttaata ttgcttctga
    72781 ccctagcagc ctaaaaccag ttttcctagt atacgacagc tgacaatctt ccacttctaa
    72841 ttcatttctt atctttttga tatctgacag gactgtccct ttagaaacct gaagaaaatc
    72901 ttgaaaatga aaaacagaaa cagacgttgc agtcgaataa accatcaaat aaagaagatc
    72961 tcttcgctct tgttcggtat acacagcttc aaatcgagta atatccaaaa atttttctgc
    73021 ccagtcgatt gctcggaatt tctttatacg gatacgggta tcatccacat attcggcaaa
    73081 aggagttaat tgtttgatcg cctcgcttgt atttacttcg atgatctctg ctgctttttc
    73141 tagactgatt tctcttggtg ttttcaggag ataactaatt ttgtagttca cagcgctgtt
    73201 cacctcctac ttatattcta gtatggtagc gcttttaaat cgaggggtgt ttggtacaac
    73261 ttacagttgg atttttttta cgactgtacc gaacgatttc tatcgaatgg atacagaaaa
    73321 aaagcaggtg accaaatgtc tcctgctttt atgaattgat ttcgtccgat caatgatgat
    73381 catgtttatt ttttgaggtc agttcttcaa atttttcttt gacatcttca accgcttttt
    73441 cgatatcttt ttcaaagtga ttgggctcat taggctttgt ttcctcttct acagaatttg
    73501 aaccagataa agctgtttct ttattttctg tttcaccaga gtttgtgcca gctaatgatt
    73561 cttctttgtc ttctgtttca ccagaattag ttcctgctaa agccacttct tcttctgttt
    73621 taggggccat tgaacctctt aatcgtttgt gtttttcacc gtcataattt gtcatgttgg
    73681 ttcctcctag tagttctata ctttttcatc gtacaataag acagtttatg cgtcaaaaaa
    73741 caaacccgat tcttacaaat atgtcatatt tttccttctg attcttgaat gcttttgatt
    73801 ccttcttcca aaattttcat ttgtaagatt gtttcttcat ctttgatgac tttttctgcg
    73861 ccattaacta atgtctcgta aatcccttcg tagacacggc tgtaatcacc aacctcggaa
    73921 attacttttt cttcatgata actcccttgt tcatctatat aggtcaatac accataatgt
    73981 tcgggcaaat cgataccaaa gtcttcatga tcaggcatgt aaaacatttt cagatgttct
    74041 tcttgtcgat cttttgtttg tttcacgaac atcccttttt ttccgtaaac gacgaaactt
    74101 ggacgttctt tgatgcggaa ataactggat tttatcgaaa ttttcatatt tccatagaaa
    74161 aaatccagat cgaagtaatc attcattcgt cctttcccta aaagttgacg tacatcatag
    74221 ctgatatcat ctggttcgcc gaaataagaa agaacttgat ctactgtatg acaggcatgt
    74281 ccatataaat agctgttcgc agtagaaaat tcatgcgtat tttctggtac ttccggtctg
    74341 aaataatcat aatgcatttc tacttctaaa atatccccta attttccgct ttcgattact
    74401 ttttggactg tcaagaagtc agaatcgaac cgtctgtttt ggtaacattg gataaataga
    74461 cctttttctt ttgctaatgc aaataactct tctgcttcct ttgacgtttc tgcaaaaggt
    74521 ttctctacta gtacattctt accatgttcg agtgccattt ttccatattc ataatgaaat
    74581 tgactaggtg tagtgatgac aactaattgg atctcaggat catcccaaag atcagacagc
    74641 tgatcagtat attcgatgtc ttcgaatgtt ttccacactg gttcagatgg ggaaaaaatt
    74701 tttttgatat tgattttgtt cgtaagtttt cttgaaaaag gcaaatgata gcgatttgta
    74761 ctctttccat tgccaagata agcgattgta agcatgagtg gtgcctcctt ttattgatgt
    74821 tttcagtata tcctagaaaa gcgcagaaag tatttataat agctattctg gtaaaaaagt
    74881 tcagaaaaac acttgttatc attcggtttg gtgaaattaa tgttcaaagt aaggataaaa
    74941 atcaaaaaga tagtagcgga accaagtgtt tatcagtaag atagaatttg gaggtcagat
    75001 catgcttatt gaagaaaaac taacaaaaca agagttgttt accacgacag agaaacggat
    75061 tgtcgattac atccgtagga atatcgaagc tgcagtctac atgacgattg aagaattagc
    75121 aaaagctacg tatacttccc attcagctat cattcgttta tgcaaaaaaa tgggattcag
    75181 cggatttaaa gaattccgtt tcgagctggg gcgggaagtt catcaattga ttgctcagtt
    75241 caatcaaacg attgatccta attttccttt ttcaagtgaa gatagcactc agcaaattgc
    75301 aaagaaaatg gcggatttat cgatccagtc aataaaaaaa gcacaattgc agattgaaca
    75361 tgaagattta gatcaaatcg gtaaagtatt gaacaaggca cagcgaatct ttctttttgc
    75421 aaaaggtgat tctcaaatca cggcaaggaa attccagaat aaaatggtca agttgaataa
    75481 gtttttgatc atggctgaag aatacagtga ttcttcttgg aatgcagcca atttgactag
    75541 cgaggattgc gccattttta ttagctacag cggtcgcatc catcattatg aacgaataat
    75601 gacctatttg aagcatgtag aggcccctac cttgctgatt accggaaatc agcattcaga
    75661 aatggccaaa caggctgata tgtgtcttgt tatatcgcag gaggaatatg attttgctaa
    75721 agtagcgact ttctcctctc aaattgcctt cgattacgta ttaaatacgt tattctcagt
    75781 gatatattct caaaattttg aagagaatat ccgcaacttg aaaaacaaac aagaattgat
    75841 gcaaaaaggc cttcttaaag aatatcggaa aacttaagaa aagatcctca tttagtaata
    75901 agaaagatag tgtttgatag gctctctttg atatcatcat aaaaacaggc tgaagttttc
    75961 tttttcaaac ctcagcctgt ttgtttacgg aacgattcga atagatatat tatagaaatg
    76021 aggaatagct aacttctgtt tcttattttg aacgattgac gaaaaagata tataagaagg
    76081 aggaaaatca gtatacatgc agctaacaat ccatacagga aaaatcgttt gttctcggat
    76141 actttctgta cttctttttg aacttctttt gcatgataag gaacccggtg ccctctgaca
    76201 agtaagcgat gactgttgac catataaggt gtacaagtta ggagtgtcac gaaatcctgc
    76261 cctttttcga ttctcagatc ttttgtatca gtcggttcta ccgtcttgat ctgatcgact
    76321 tgataagcga gtgtccgtcc attgatttcg atatagaaag aatcacctaa agtcagtttc
    76381 ggtaagtctg tgaacaactt tgcttgaggc agtcctcgat ggctggaaat aacggcatgt
    76441 gtattttctc cccccgtagg ataagacgtt ccttccaata atgaggcacc tttttctagc
    76501 agcacagtat tcgtttcatc aaaaattggc aaatggacat tgatctcagg gatcgtcaaa
    76561 ataccgatcg tatgtttctc aaaataggtt ctatcctttt tcgcaggttc ttttttctta
    76621 gtaaagggat ctgcacctgg atttccacct tcttttgcta attgctggtt tttctttgtc
    76681 atgttttctt ggattttagc catgacttct tcattttctt tgaccgcttg ttgctgataa
    76741 tgagagatga tctgctgatc cagataatta ttcagcgcgt cgctaacaaa tggataagct
    76801 agtatcccta ttcctgagag aagcaagaaa agcatcagca gatctagaag tttccttttc
    76861 tttggtttca tttgtgttct ccttgtgatg actgtttgat aataaaagaa aaaggagcat
    76921 ttgcgtaatc cgctgctcct ttttccaatt cttgcaaaaa gactaaatct gactgtgctt
    76981 gcgtctagca aagtacagtc cagcaatcag tagaaggact actcctgcac cgatatacac
    77041 atagattccc ttaccgcctg ttgaaggaag tgtacctttg tgtttatttg gtattttttc
    77101 aggagaaatc aactgtcctg ctgttacata agattgttca tcgatagtaa atgctacacg
    77161 gtttgttaat ggaacatatt tttctggcgc tttcgtttct tccagatagt acgtgccata
    77221 ttttagacct gtcacatcga ttaaaccgtt acttgtggtt gtaaaaaccg ttgctgattc
    77281 tttcgccgat acccagctga cggcttttgt ggatgggtcg atcgataaat atttcgctgt
    77341 gtcactatct tgatcacgaa cgacgaattc tgctccagca agtgtttggt ctgatgtaac
    77401 gtcaccatct actttaacaa atcgtttgcc cccagtaacg acatcgactg acggtggtgt
    77461 ttgatcattt gtatgcccgt tatcgacatt tgcttggtta gaaaatcctt tggttggatc
    77521 tgctttttcg ttcaaatgca tatagtaaac gaatttcaat gtaccgccag gagttaatga
    77581 aggaatataa ttcggatcaa ctgaaaccgt gaatccgtct gtttgctcag tgacagaata
    77641 atttactggg tcgatttctt tatttccatc atataaggca taagcatacg ttcctgaaga
    77701 atcattatca aatgttaaag cagcgtcatg agtatcgata agtttgaatg ttgtgtactt
    77761 gttttgcgtt ccttctttat cagcgatacc taatgggata ttgacagaaa tctcatattg
    77821 aattttttca ccgattgcga catcttttcc attcaattga ggtgttgttt tatcaacttt
    77881 agacgtatca tttttgatgg tcttttctac tggtgtttgg ctatttgcca ggatctcaaa
    77941 aggcgtgatt gtttgctttt caatcatttc cgcattatca ggagctttta cttcttctaa
    78001 attatatttt ccaacttcta aatgattaac gatcaactgg cctttttcaa tagatgcctc
    78061 ggcaaagtca ttgttgccga tgtcataaga atgaccagta atgaaatgtt tggctttggt
    78121 ttgatcagtt gtccaagtgt acaatccatc tgtgacactt tggatgtatt ttttgacgct
    78181 tggtgttcct tcttctttag aaataataaa ttctgctcca tttagtgctt cgttttcggc
    78241 agtaccgatt tttgtaactt tcaacgttcc atcattacag actgtatttt tagggtagag
    78301 atggatagta tctagttctt ctgtcccgta tttataagag ccatctgctt gtttgatcat
    78361 ctcatataca gggaaagcta aaaccatgtt tgcggcagct gacactccgt cttttggttc
    78421 ttctttgatc gtgtagactg catctttccc attttgtttt ttaggtaaag ataaagtgac
    78481 atttccatca gcatctgtcg ttcctgaagc aactggtgta ccaggagtca aagactggac
    78541 tgcttgtttt gctgcatcca cggacgctcc tttatctcgt tgcgcataaa attcttgagt
    78601 gacgttataa actgaaaatg aaatatcggc taatccttgg tattgatcga attcgctcat
    78661 ttctttcccg ctgttttgga ttaaaggatc gggtaaatca gtcattttct ttttatgaag
    78721 gatgacttga gctgctgtct cctctgcaaa cacttttttt gcgccgaagc tgtttgtgag
    78781 taatggtaaa ataaggaaaa ggactcctaa cataacgttt atttttttat gatttttcat
    78841 tttttcactc ctgttctttt tttgataaac aaccagattg caaagcttgc gaataccata
    78901 ccaacaacgc ctaaaagtgt atttttcgtt tcgtttgttt ttggaagaaa tggacggcta
    78961 ttttcttttt tattgactac tttcaatata ctgtccgcct ctttatcctc agaaagaaaa
    79021 gttgaaatgg taaacggaat ttctgtttca cttaaaatat aaccagaagg tgcttcgatc
    79081 tcttttaatc gataacttcc tgttttcaac ccggaaattg aaaaagcgcc atttttatcg
    79141 gaaatcaggt gaagcgcatc tgattcgttc tttgtccact tgtatccgtt ggctgtttgc
    79201 tggaggtaat ttcctgcttt gtttttaata aggaatactg cgccagctaa tcgtttatct
    79261 ggtttatttg tctcgatttt gacaaattgc ttactccctg tatatacaga gactttcttt
    79321 gtactgacac caaaaccaaa atccagtgtt ccgttgttgt tgatttcctt gttcgcctgt
    79381 gctgtactgc ttagctgcat ctgataagaa atggtcagtt tttttccagc atatttttcc
    79441 aacgaaggga tcgaaaataa aacatcaaat ccgtgaggtt tcttgtgtat gacaaagcct
    79501 tctgtcagct tttctccatt cgatgaaatc gttaaactat taggtaaaaa cgttaatgct
    79561 tcatccgcac tatctgacaa ttcgaacttt tgatagtcaa ggatatttac aggaattgtc
    79621 gtatgcaagg aataagtgat tttttcacca atcgtaaaat catttctagg ctcttctaat
    79681 accttctcaa aaggtggatc agggtagtcg ttttcttcat ttttaggata caaatggata
    79741 gttgaaagtt tttgattgtt ttgtccatgt acaggaagaa caactaccat attctctgcc
    79801 ttttctttga cgacttctgg tactttggat tcaatgaaaa gatacgcttt atctcttttt
    79861 tcttgatctt tagcggccag agaaaaagaa gcgataccat cttcgttgtt aagagttgta
    79921 gttgttgctt ctgcggtaaa cataccggaa gacgcaccgt tttttgcgat ctctgcttgt
    79981 gcttcttcta ccgttttgcc cttttcccgt agatggtaaa aagaatctgt gacatcataa
    80041 acttggaatg tgacaccatt taatcctcga tacgtttgta ataaagcttt ttcttgtccg
    80101 tcatttggat gatttttcgg cagttgcccg tttgggaaaa gcaatttgtg taaagtaatc
    80161 tgaaccattt ctgtttctgt ttctgttgct acctgagtaa aagctggttt aaatagtaac
    80221 aagaagagac acatactaag ccaaccaagt tttttcatat ttagctgccc cctctttctt
    80281 ttttcaaaaa aagataccaa atagaaaacg cacaaccaat catccctact agatagattc
    80341 ctaaacggcc aattcctccc gtttcaggta atggtacttt tgcctgattc gtgatgtcta
    80401 aagaaatctg gttgtttttg gctcctggtg acagaacaga ttcatgatct tgtccatcca
    80461 cttgaattga cccatcttcg tgtataacta tagtaactgg ctcttttaga ccttggtatc
    80521 cttctggtgt aaaagtttcg gtcagcgtat aagttccagg ttttaaattt tcgaatagaa
    80581 aggtatctgt ttcttgtcca tcttttggcg attctaagtc catttctggt ccctgcagtc
    80641 tgaattttgc tccttttaat gtctgtccgt tgtcttcttt tttgtgtacc gttagatcaa
    80701 acgcttttag tcgattttga tgagtgagcg tccatacatt atttgtcatc tcggtatttt
    80761 ttcccgtgta aatgattttt ccatatttat cgatttggaa ttcataattt cccggcgaag
    80821 tatcatatcc ggcaggacca gctgtttcaa cgactcgata tttaccaggt gtgagtgaat
    80881 caaaactggc aagacctttt tcattcgttg tttggctgtc aattggctga taagtaccat
    80941 ttgcttcttt tttctgtagg gaaaatgctg ctcctttaag atttatctct ttgtccgtcc
    81001 cttgcatcgc atacttacgt actgctacag gaacttcaac aaaaggattt tcaatagtca
    81061 actggatcgt atcatcggaa gtagtgactg tttttccatc aatggttacc gtaccatcag
    81121 aagcgatctt gatttcccaa gtagtctttt tgcttaatcc atgcccttct ggagcttttg
    81181 tttctgtcag cgtataggtc atttcttttt gcaatttgac attttctgga agagaatagg
    81241 ttccgtcgcc atgatctgtt agtaaagtat caatagaatc tcctgttaat ttgaaaacag
    81301 cgccaataag atccttttca cctgtagagg aatttttcgt tattttcaag tttaacggtg
    81361 tgaattgttt agtatttttc catgtcattg catctatttg ttgagaatcg tatcctggta
    81421 caggtaattc tttgattgtt tggtaactga atgcttgacc ttgattattg tattgaggta
    81481 gtgataatat ctcttgataa ctttctccgc tatttgcaga taatttgtca atgtctgcac
    81541 gttcccacgt atttgttgta tctttagctg gtttaatgat tcgaatatat ccgtttttcc
    81601 aagctgcagc atttgtcgta tgttcccgtt gaatctcaaa agtaacttga tctggacgat
    81661 cagctagatt gttgtcaaat tcttcccata actttttgat gtgaagactg actccgggag
    81721 ctttagcaga tggtataccg aattcagcta attcattggt atcaatgttt ggctggaaag
    81781 ttgtcctgcc gttcatttga taccagaaat ttggatggaa gtcctcattt tctgtttgga
    81841 ttctcacttg gtaatggatt tggatttctt ggtcttttcc taaatagatc tgattgctct
    81901 tgatggtatt tccttctatg gaaatagatg gagtgaccgt tgtaggactt gtccccacac
    81961 tcttgactga aagtgtacca ggctgataaa tgaatggttc tgcgattgga tcatttattt
    82021 gtccattgct tacagttgct gagatctgta cagctttttt ggctaggtat tcagagatat
    82081 ctgttgcatg atcagctgat tcatagtaaa gatcgccttt ttcatctgat gaaaccattt
    82141 gtcgcatacg agactctact tctgcttttg agagaccagc tgccggatcg ctttgaagtt
    82201 ggatgccaag accatgtatt tcgattcctc gttctttgag tgccatcgct tctccgatgg
    82261 ttgcgataaa cgtactgtcg attcttctgg atagattgtt ttggtcaggt gcatcataga
    82321 ttcttgagat tagagaagta tttcccggcc gatcttgcgt attagaaaac tgagttccgt
    82381 aataattgct gcttgattgt gcgtgtaccc gctgtacttt ataggaaaat gttggtacac
    82441 catccgtcag caaaacgatc acttttttat gtccatttgg aacggatagc atgcttcctg
    82501 catctcttag tgctttttgt gtaaaagtac caccatttgt ccgtgaaggt gtaatggatt
    82561 ttacttgatt tttcactgaa tcaaatgaac ccatctgtac tgcaccgtta ctgtagctat
    82621 atccttcgct tgagtagccg acatatccca tattgatttt gtctgtgata ccgctatctg
    82681 ctaaagtatc gacaaaacga tcgacaccaa tctttacttc accgatccga ttattgtcgt
    82741 tcatacttcc tgaccagtct acgaccaata cgagatcaag aggcgtgata tctttttgta
    82801 catttccacg agcattcaaa taaacatcaa acaatccggg tgtacttgtt tcctttgcat
    82861 actttcgtat cgcatagtct gcttgattac cggttcctcc atattcgata tacgaatggg
    82921 tccgatcatt aggatctcca ttccaagaat ttataccatc ccattgattt gttccgtttt
    82981 tattgccttg atgatcaaga acatttgtat ttcctgtagg ttgccaactg gctttaggat
    83041 aagtgccctg atcatctgtt gtatattctg taggaatcgt ttgattagat ggcacactca
    83101 cagctggcaa ggataatgta ggcaatgtaa atgggctgac agaattttcg ttttcttttg
    83161 atgattcttc ttctatttcg tctggctttg gtactgacac atctgtatct ttcttatttt
    83221 ctgtctcatc aataatgtca tattctttat tatcgacgat cagcttgttt gcctggctgt
    83281 ggtaaagtgc tggatcgatt ggaaaactca gatcgatagt ttgtttagtg tttgatgaga
    83341 tttccaacgt aattttcccg ctatttacct gataagcata gccatctcct tcttttcttt
    83401 ccacatcact taagtaattt tcgactagac caatttcttt ttctatcttt tcagcactgg
    83461 tgttattcgc atttagcttg atgttcagtg ctttttttcc agactgatcc gaaatgactt
    83521 cagctgaaat tgtttctaaa actgttggat catctgtagt gtctgctatt gcagataatg
    83581 gcacaaagtt tgataaactc aatattatca gcatgaaaat aactttcagt ttcttccctg
    83641 tggtcgtcat ttttcccctc ctttttcttt ttttctgttt tgacgtgaat agtgaaaatt
    83701 attaaaataa acaggcgtta cataatttta tttattttaa tatgataatt attatacgaa
    83761 gggcgctaaa gggaatcaca ccaattttca taccaattgg tttcatttat gaagttgatt
    83821 ccttcgatta atgagcgttt cattgaaaga aaagggttga gggagaaaaa catatgatca
    83881 ggttgttgga atgcaactat agaaagatgg aaaaaatcat tcgaattctg tccatttctg
    83941 acaagtggat gactaaaaaa gaacttgcca aacgaattgg tagttcagaa tctacatttg
    84001 taaggtatat cgaagagatc aaaatccgct ggggaacgga tgtaacgatt caatcttcac
    84061 aaaaattggg gtatcgtctt gaacatttga atgtaccagc ttatttaaat gttctgatgg
    84121 aaatgtcgca aagttctaca aacagtcgtt tattaaatga gattattgtc aatccgggta
    84181 aaacgattga gtattattgt gaaaaaattt ccatcagccg gtcttctttt acacgtaagt
    84241 tgaaacaatg caatcaaatt ttagaaagct atttattaaa agtgatcgtt gaccaaggtt
    84301 ttcagttaac tagccttaaa agtgagctcc agttgcgtat ctttgttacc tttttctttt
    84361 taagttacta cggaagacgc acgttgccat ttccaatcga aaaaaagaag atcaaagaaa
    84421 ttatggtcaa gaataattgt cagatgaatt tgtttcagga agaaagccca tatgagcaat
    84481 ctttctttat tatgtatttt gttgtgcatc tcatgagaga atcccaagga actccatttg
    84541 aatggaaagc tggagactct tatgtacagt tgataaatat cgataaaaaa gacttcttaa
    84601 tcttgaaaaa tctcttagga aaattgaagt acagcagcta tataaaagta gtgaactatt
    84661 ttgtcaatac gattttttca gaattttcgg agaaagaaaa aaatgaaatc agtgagcaga
    84721 tacgaaaaaa tatgtcctac tgttcgtttt catccgctga ttgcaaagaa aaaaacgaag
    84781 aatatgttgt tcaattatta acgcaaattt attttttctc ccgtattttc ccatacgata
    84841 cgactcattt gacgatccgt gcaacttact ttgcacagca gatgcaacac cgccagatac
    84901 gtctatatgc tggatttcgt gcagaacttc aattttattc cactctactg aatcagaatc
    84961 tgatttatta tttatctagc tatatctttt ggatgcttat cggtaatcct gaaattcacc
    85021 attatacttc tgataaaaaa atactgatta tcagcgactt aagtcttcga catagtcaat
    85081 atattgaaga atatatcagt gatattcttg ctgtgcataa aattcactct gagacaactg
    85141 ctattactga agatcagctg tcgaaataca atcttctcga gtatgatctg atcgttacga
    85201 atcaacccat tttgaatgcc catgttcctc atatattgat tgatgatagt gtgtcattcg
    85261 caaatgaaga agaactcatc cgcttattcg agttatgaat aatcaaaaaa cagctatgta
    85321 taaaccaccc tgataaaaat ttcaggacaa atacacatag ctgtttcttt ttattgtatt
    85381 ccttcagcaa tccaacgaga tttatagcga aagtctttta acaactcttc ttttgtccaa
    85441 ttatttgcaa tgagatccgt caaagtgttc attacgaggg tcatggaaaa gtataggaaa
    85501 ctaactgctt cttccgacga ttgaatggag aaatgattga ttcttaataa ttccagccat
    85561 ttttcgttca ttggagaatc agccgatagt tctctacgtt tggttgtctg ctgatcattt
    85621 cgctgggtta acaagtataa cctttgccaa tagatacttt cgtgatctaa gtcgcagcac
    85681 cactctaaat atttttcgat ccccaaaaag aaatgttgtt tgttttcgct aatgaatttt
    85741 aagatatctt gatggactat gtgagaactg cttttgatcg tatacaaata agcatcttcc
    85801 aaatcttgaa aatatttata aaaagcaccg cgcgacatcc ccatcttgtc aacgatttct
    85861 gagactttga cttggctgat gtgctgtgag caaaatatct ccagtaatag gtcatcgatt
    85921 ctctgtttct ttgcgttcga taaattgaaa aaagtttctt ttggcatcca catctctcct
    85981 ttatctctga ttatacgtgg atagcccttc attgtcatga aggattcatt ccttcttttt
    86041 ctcttcttct gtcaagtacc catcttccat tcgataaacg cgatcactcc attgaatcat
    86101 acggacatca tgagtaacca taatagtcgc tttttgtttc tcatgagctt ctttaactag
    86161 taatttgacg acttcatatg catgatctgt atccaaacta gctgttggtt cgtctgctaa
    86221 gatcaagctt ggctcattat ataaagcgcg agcaattgcc actctctgtc tttctccacc
    86281 agaaaggtct ttaggataac tatttttaag tgactggata tctaaagatt gcagcaagga
    86341 atcgattttt gttgaatctt ctttttttga agtaacttgg tcaactagtt caaattgttc
    86401 ctttaccgtt aaaaaaggaa tcaaattaga gctttggaga ataaatccta tttctttaaa
    86461 gcgtaaatct gctcgttttt tttctggaag ctcagttagt gagtgaccgt tgatcactat
    86521 ttctccggaa gtcggtgttt gcaatcctcc tgaaatggta aggaaagtac ttttacctga
    86581 accagaagga ccaatgatgc tgacgaattc tccttccttt acataaaaat caattgcttt
    86641 caaagctgag acctttgtat gaccagaacc aaatgttttg gtaacttttt tcatcgttaa
    86701 tatttttttc atttttatta acctcctatt gctgtaattg ggtctacttt ggttatcgta
    86761 cgtatcgaaa acaatccgcc tagcattgcg acaatgatca aaatcccact atatagcaac
    86821 cattgtgacc aaaaaacagc aaaaggcatc gcgcttggta aaatcagact agtcaaataa
    86881 gcaaacaaaa gagcaagcag aacgcctacc accccaacaa taaaggactg tgctactaaa
    86941 gatttagcga tgaagaaatt tttgatccct tgtgctttca ttactccaaa aatagcagtc
    87001 ttctgaagtg tcatcacgta catgaaaata ccgaccacgg cagcaaccac taagaacaaa
    87061 aagtatatca tcgcatttaa ggtcatattt tgggcagaat agccaggaat actttcaatg
    87121 aatgtcccaa tagttaataa ctgtagaccc ttcgatgctt tttctttcga aattttagct
    87181 gaatccttta ttacgatccc attgatcggc tgttcttctt cagaagcaaa cggctgttgt
    87241 ccatacttca aagcagtcca cgtatctagg gacgcataga tgacgggaga aactgtgtaa
    87301 taagtttcag gaaagatacc gacaatcgtc aaaggatcgt cgtaactgcc aattttgatc
    87361 gtatctccaa ttttatagcc tgaatcagcc agattttgag agatgatgat ttcattcttt
    87421 gttttaaatt catgcccttt cgtgagtttt ggtaataaaa atgcttcttt cgttgtgccg
    87481 aaaactgtaa cattttcttt gttttttcct ttgattgctc cgctgtacaa tccgattggt
    87541 gctttctctc ctccttcaat gtatttcaga tctcctcttg ttaattgtga agctgcaaat
    87601 acttgatttg catcctccga caaaacaatc tcttgtgcgt cccaatcatc gattgctttt
    87661 ttaaactctt ctgaaagtcc attcgctagt cctgacaaca tgaatacgac atacgcaatc
    87721 aagaacataa taccgacgat caagccatat cgtaatttag aatagcgaat ttctttgata
    87781 gctagaaaca tttgtttttc ctccctatga tagattgata gtgacaaaat gtcacttgtt
    87841 gaatagtgta actctaaagt gacaatttgt cacttgtgtt gtttttttct tatcaataaa
    87901 ttgtctaaga catctttcga taaaacctta acatgagtca taaaaaattt atttaagcct
    87961 tatttatgct ttccatgata aactaaagtg accaaatcaa tgaggtggga aaatgattaa
    88021 actattagta gtagaagacg ataaagtatt atccaataat ataaaagaaa tactcgctgc
    88081 aatcggcgag ataacacaag tttatgatgg tagtgaagga ctgttcgagt tggaaagtgg
    88141 agtttatgat cttgcaatct tggatttgat gatgccggaa atgaatggat atgaagtgtt
    88201 agcaaaaata cggaaagaaa aaatacagac tccagtattg attttgactg caaaagatag
    88261 tttggaagat aagattgaag gatttgaacg aggtgcagat gattatctaa caaaaccttt
    88321 ctatcgagaa gaactgatca tgcgcgtgaa agcattattg aaacgctcgc taggtctctt
    88381 tgatgaaaat gttctgaagt ataaggatat cagttgtgat ttaaaaagca atgaagtctt
    88441 gtataaagaa caagttttgc ccatacaagg aaaagagttt gaattgctcg tttatttttt
    88501 acaaaataag ggaatcattt tgacaaaaga gcagatattt gaccggatct ggggatttga
    88561 ttctgatacg acgattacag ttattgaagt ttatatgagc aacttacgaa aacatctgcg
    88621 aagtactgga ttggataaag aaatcaaaac cttacgtaat gtcggttata tactgcaaag
    88681 ggtgtgatgc aatgaatgga gatattacac gaaagcaacg tagtcgcttt tttgcgacaa
    88741 atgtagcagc atttgctttt atcttttttc ttttgggatt cattacttta cagatattaa
    88801 accaatctgc atacagagaa acagatctgt cattagagaa aataaatttt gattctcgaa
    88861 cagtacaatt agagatacaa agatatcagc aaggcgatcc ctttttaaac ccgactcaaa
    88921 cggaacaaaa cagaacaaac atggaacagc cattaatgga accatcaaat gaccgtttta
    88981 acactcaagt gatcttgtgg tctaaaaatg gtgagatatt gaataaagca gctattggtg
    89041 ggcgtttcaa ccaaatcaaa gaattaaaaa tggatacgaa aaatctgaat accatccaaa
    89101 atattgaatt aaatgaagac agtggctcat ctgccctctc tttccgttcg attacgaaaa
    89161 aagcggagac gaatcaagat ggcgttgcct acatccaaat tttagaaaat acaaatcaaa
    89221 tcaagaattc attagcgact tttcgcacga ttatgatttt gtgtatgatt gttttttgga
    89281 ttctctctat tggtatcagt tattacttgt ctagtctaag catgcgccct attattcttt
    89341 cttggagaag acaaaaagaa tttgttgaga atgcttctca tgaattgcgc acaccgttaa
    89401 cgattattca aaatagtttg gaacatcttt ttaccaagcc taatcatact attattgaag
    89461 aatccgaaag catcgctcaa gcattgtcag agacgaggcg cctcactgga ttgacttcgg
    89521 atttgctgac aatcgctcgg aatgattctg atcagcagct gctttcaaag caaatgatca
    89581 atacacaaga atatatcgaa caacttgtta aaccatttca agagatggcc atcatagatg
    89641 gaaaagagtt tattctccat aacgatgcaa cattagaagt accaattgat gaaaaaaaaa
    89701 tccatcaagt tttggttatt ttgctggata atgcattaaa atatacgaat ccagaagata
    89761 agatcatttt ggattcatct gttaaccata aagaatggct gattcacgta aagaatactg
    89821 gcccttctat cagtgaagaa gatcaagcaa ggatttttga tcgattttat cgggaagatc
    89881 aatctcgggc gaaagagact ggcggatacg ggcttggctt ggcaattgca aaacaggtaa
    89941 tcgaacagca tcatggacag atttctgtat ccaattatct tccacaagga gtcgatttta
    90001 aagtccaatt gccgttgaag cctaaaaaat aatgcttatc cctaaaaaag ggctgtgaca
    90061 aaaatcttgt cacagccctt ttttgttgcg aataaacggt gttccagaag tagcttcttc
    90121 ggaaataagc cgaaatttct ttaagctgac cacttctgtc acaacctctt tagcttacgc
    90181 ttacgatttt ttattgcggt cttcaatgtg tacatcaccg cttgtgtcaa tatccaattc
    90241 ttctcggcta acatcttctg tcactttttc aacatcttca tgttcttctt tatgaatccc
    90301 aacttcttca cgaataactg tatgtttgct tacatctact tgctcttctt tgattgggat
    90361 agtaatcgtg tcatcttcca atttttcgtc tgttccctgt gaagattgat ctgttactgg
    90421 tttgcgttcg atgacgattt cttcacgttt gaccggtact tctaccgttt cagtatcgtt
    90481 gaccacatgt ttgtggatgt cgacttctcc agttgtcaca tcatgtgtat ttacatctaa
    90541 tttttcttct tttagttgaa ttgtatcatc atctagattt ggtttgcgat cgatgtcttc
    90601 agtcttgctt gtacccattg tatcagtctc gccaggtacg tccatcggat cagttcctgg
    90661 tgtcatattc aatggatccg ttgtattttg aaagcctgtc gtgccaccta cgtccattgg
    90721 atctgttcca gtgccagttt ctaatccagt ccccattgtg cttgctgggc taacagtatc
    90781 ttgtgtatcc attgtatctt ctgttgtttc tggctgacga tagcctttta ctgtgatgac
    90841 aaattttcca tcagaaatat ctcctctata ttgatataaa ggatcatttt ctggtgtagc
    90901 cgtctcagaa tcataggtat caaaagaaaa agtatctttt attttttccc ataaagaacg
    90961 gtcttcttca ccatccatcc gattttcatc tgtttcaaca tctataccag acaatccgtc
    91021 aaatcctagt ctagatgctg cttcatcagt agtataaata acgatatcat ctcgttcata
    91081 cccttcgtta cgcaaccgtt caattactga taatgcttct tccctcgtac tatagcttcc
    91141 gatgaccgtt aaatttttct cttccatgta aattcctcct agcaaataga tttatgattg
    91201 gtatattgtt acttataaat ttattctata ggagacgatt gttttctaca aatatttaga
    91261 ttggaatttg attaaaacaa tttaattgaa atttgaaaac atttatgtta atgaaagtca
    91321 tattctaata aaatgtcatt tgtttctata tgaattttta tgaaaaattt catataattt
    91381 ccactcgttg tgatggttta atttatccaa tccatcaata agcaaacaac ttatataaaa
    91441 aaggattaat tctcaatata attgctccta ccatagaata aatcttcatg taaagtaatg
    91501 aagtaccaac tatttctaat acttctttaa gaattatccc aacaatatcg ttagacaaac
    91561 cattattgaa taaatggaga gccaatcgat tctgaaaaaa tgttttctaa tttttcggaa
    91621 ataaaagaaa gagcctcctg ccattagaac gaaccaaatc catttcattg ctcatgaagt
    91681 aaccacatag ctaaaaagag gataaatcaa ttaaaagatc aaaagaatga ctgcactaat
    91741 aaaataacta aaaatgtatc ttcttacttc atatctttta gctagtactt agttaaaaat
    91801 ttgcttccta agatcaagac aaactgtcta tcttcatttg atgaaatgcg gtaaaatata
    91861 gaaaatgaac actattggag ggaatttgta atggacagca aaactagaaa acaactgcaa
    91921 gaaaagttaa agcaacgtac acacttgctc ccttccgtct ttcaacagtt tttagaagaa
    91981 aatcgttctt ccctttcatt aggcagtcgc tatgaatacg caaaagattt tcatctgttt
    92041 actgattatt tgagaaaagt attttctgaa gaagtggatg atcagctgat tatccattta
    92101 gaaaaacaag attatcagaa tttcttagct aaaatctatc gacatactcg ttcgttccaa
    92161 actacagcga accaagaaac ggagcaattg tttgaaaata gctattacgg gatttcaagg
    92221 aagcaatatt cattaaatca ttttttacga tttttgtatc aaaaaggact gattgaagaa
    92281 gagatttctc ttttgggtac aagtttaccg gaaacaacca aagcagctcc gcgatatatc
    92341 gatgcagaac ttttcaaaga ttttgagcat ttgtttatcc cgattgaagg ctttcaaaca
    92401 agcagagaag ctagctttga agaaaagaat agaccgagga accttgcaat tactgccatt
    92461 cttctgtatt gcggattaaa gatccatgaa gtagtagaac ttaaacgtaa agatgtatct
    92521 ttgaccaagc agattcttaa tctgaataga aaagaaaaca gactgaataa agtaccaata
    92581 ccaaaagaag cgatgccatt tctggagaac tattttaagt taacagcaca aaatacggac
    92641 gaaaattctt ttgtttttcg ttcctcacat gatcagcaaa tttctcctcg gactattcgc
    92701 caaattttga gcaaaattac agtatataaa aacgtatcgc cagaactatg cagaaaaaca
    92761 tatcgttatt atgcagaact atatttagat gatgctgacg cggtaaatta tttatgtggt
    92821 aatcgatacc tctcatccca ttcttttcaa gatatctggg aaaagatggt tgatttttct
    92881 tatcacgaat tagcggcatt cgttcgggtt tcttccatat aaaacaaatg tatgatcttt
    92941 ttttcacaat aaactatgtt attatataaa ggattatgtt acgattatct tgaatttgta
    93001 tattacttaa caaaagataa tggagtgtga atgatggaga ccataacgta ttcccctttg
    93061 aacttattta cgaattttca agaagctgca gctaactttt ccgagacccc gattatcttt
    93121 gatgagccac tttcagcttt cccagaatta ggacttgaaa caacttacaa aaaatgctgc
    93181 gaaagtgtgt tgaaacgggc ttatcaattg gctcatcttg gagtgaaatc cggagacaaa
    93241 gttattattt ataagagttc cgcttttgac acataccttc tagctgtttc tgtgtcttac
    93301 cttggtgcag tgcctgtcat gacttcttat catctaccaa caacaacaat ggaagtattt
    93361 atcgatcgtt tagaagaccc tttcatttta tttgatgatg agacaaaaaa gcgagtgaga
    93421 gaaatttcaa atggaacaaa atcaaaacag attcctattc tttatttaat ggaacaacct
    93481 gctttgtctg tttctcaatc atttttagac aaaaatgaaa tttcttatat gacccatact
    93541 tctggtacaa ctggtatccc taaacttatt tgtcattctg ctcattctat gggatggcgg
    93601 acgaaatggc aaaaaacagt tttcacaaaa atttcggaaa agaaattaat cgcttttcac
    93661 atctctccag tgcattccag attcaatatt ggtatctctt ctttgatgtc catgggattt
    93721 cctatgatgc ctttagcaaa cgcacaaagt tcaaaagttg ttcatatgtt ggaagctcac
    93781 cggccaattg cactagagac ccatccgaat aactttgttc agtggcgttt tactgctaag
    93841 gagcatccag aagctttttc gggtatccga tactatcact ccacttttga tgctataaac
    93901 aaccagacaa tgaagacatt tttgcgaacg tcgatggcac aggatgcggt atttcttcaa
    93961 gtatatggcc aaagtgaatg cggaccaatg attcttaagg ctcatacact agagagtttg
    94021 aaaacaagtg atgcaagaga tatgggcgtt ggactggaag acatgacaag cgctcggatt
    94081 acagattcag ttgggaatgt cctacctgaa aacacagatg gtcatataca attactgtct
    94141 aaaggacggg ctttgactta ttacaaggaa gatgcacggt tccaggaaaa tgtttatggc
    94201 gattggtggg atagcggaga ttacggtttt atggataagc atggccacct atttttgaaa
    94261 gatcggcaag tggatttgat cgaaacgatc aatagtacat tagctattga agacttttta
    94321 ttagattcgt tagatttttt ggcagaagta gtgattgtaa gggataaaaa tcattctcca
    94381 caaccgatta ttgctttagc tcctgataaa gaaatggatt ggaatcgatg gtgggagcaa
    94441 gtccatgaac ttcctcgctt gaacgtccct atcatccgtg attttgatgc aatcccgcga
    94501 acagctacaa tgaaagtgca acggcttcaa atcgagaaag aattaaaatc acaaaagtcc
    94561 taactattta aggaaagaaa gatccatttt ggtctttctt ttctttttgt ttttttaaat
    94621 tggatgagat aaaaatatgc caattggctg tttttctttt ttgaaaattt cgttatactc
    94681 ttattcatgg aggaaaatgt tatgaaaaga atcttgacaa ttggcggttc tgatccattt
    94741 gctggtggag gaatccaaac agatttaaaa acttttgaga attttggtct attcggacta
    94801 agcgccttga cttctatagg ggcactagat attaatgagc gatttatgct ggaatctata
    94861 tctttggaat tgcttaaacg acagttagtt tcgattgata aaatgacttc attagatggt
    94921 atcaaaattg ggttattgaa ttcaagtgaa gcaatcatgg ttgttcgaga cttcataaaa
    94981 acaaaacaaa atatcccaat cattttagac cctgttttgg cattcaaaga aactgaagct
    95041 gtgatcaatc aattgtatat ttcgcagctc atcgaagaac tttttccact tgttacgatg
    95101 attacgccta atttggcaga agctgcttta ctttctggtc aagctcttcc ctcttcgtta
    95161 acagaaatga tccatctcgc taaagaactc actgcttcag gaacaaaaaa cattgtgatc
    95221 aaaggcggca caaggatggc tggagatgaa gctgtggatc ttctctattc aattgatcat
    95281 tatgaggtat tttctcataa aaaaatcgac actacaacag taaacggggc cggttgtgcc
    95341 ttttcttctg caataacagc gaatcttgtg ttaggggcag attttaccaa agcagtagca
    95401 gaaagtaaaa aatttgttgc ccattgtatt tctaatggcg tgctgatgaa cgatcatacc
    95461 ggaagtgtct ggtctggcgg tattcaaaaa ggaggaatga gaaatgaaaa ctaagcaatt
    95521 aacgatctat gctatgctga ttgcgttgac agttgccctt tcattaacaa ttttgatccc
    95581 tgtaccggct acgaatggat ttgtgacatt atgcgaggcc gggatctata ccgctgcttt
    95641 tctttatggt ccaacgggtg gattagttgt tggtgcttcc agtggtctat tgatcgattt
    95701 gatttcagga tatccgcagt gggcagtttt ttcattagtg atccacgggt tacaaggata
    95761 tgttgctggt tctttcagca aatcttctac tcgtatgtgg ggattagggc ttttattagg
    95821 aagcttagtt atgatcatcg gctatggtat cgccggttgg attttatatg attggccggc
    95881 tggagtggct tctataccag gaaacatctt ccaaaatctg attggaatgg gacttgcctt
    95941 tcctataact gcaagtcttc aacgtattca agcacggaag caatattaat tgaaaaagaa
    96001 ggttttagaa aatgaagttg tcagaaaaag aattaaaaga ccaattgact gaaatagtta
    96061 atgacatatt ggctgaggca cacttgaaaa aaggtgatct ttttgtttta ggatgcagca
    96121 caagcgaagt cgttggagga catattggaa aaaattccag tgcggaagtt ggtcaatgga
    96181 tcatccgtac gttaaaagag ttgttggatc ctaaagaaat tgcgctagct gtccaaggtt
    96241 gtgaacattt gaaccgagca ttagtagtag aacgaacagt ggcagaagca aaaaatttcg
    96301 aaattgtctc tgttgttcct gcactacatg ctggaggtgc ttgttctgtt gctgcttttg
    96361 accagttcaa tgatcctgtc gaagtggaac acgtagttgg acaagccggt atcgatatcg
    96421 gtgacacatc aatcggtatg catatcaagc atgtccaagt acctgtcaga cctcgtttga
    96481 aaacccttgg acaagctcac gtgacagccc ttcgctctcg tcctaaatat atcggtggtc
    96541 ctagagcgaa ttattgaaat gagtaattga cagagcaatc tgaaaaactt cctcgcaaag
    96601 tccaactaat gaactttaac gaggaagttt tacttgtttg ttttatataa ccgagcttca
    96661 taaggtgcta gtatgccttt agcttcaaat tctccgctag aggaatttga caagagtaat
    96721 gtccaggact gatctgcttc ttttggaagt tcatagtcag caagtgattc tgttaagttt
    96781 accataataa gatattgttc atcttctaaa tatctttcat agacataaag ttgtggatgt
    96841 tcatgaagat attctttata agaaccgtat atcagacctt tattggtttg tctaatgcgg
    96901 atcatgttct tatagaattg caggaccgac tctggttcat attcttgttg ttctacattg
    96961 atcgttttgg tatttggatt agtgatcagc caaggttcat gtgtagaaaa accagcatat
    97021 tgttccgaag tccattgcat gggtgtcctg ctgttgtctc tcgttgtttc acgaatgata
    97081 tccaatgctt cttctcttgt cttaccttcc gcaagcagcc ttttataaag tcttttcgaa
    97141 tctactgcat caacttgatc gatcgattca aagggcatat tcgtcattcc gatttcctgt
    97201 ccttgataaa taaatggcgt accttgcaaa aagaaataca tcatagcgat tgcttttgca
    97261 gacatagccc aaaaatcggg acgagtatct ccaaacactg aaacggcacg aggaacatca
    97321 tggttttcca tatataaagc gttccatccg ataccatcca atgaagtttg ccaagcgctt
    97381 aatgcatgtt tcaatttaag gacatctaat tgcccttctt tttcttgctg ccagatatgg
    97441 atatgatcaa attcgaatat catatcgaaa taaccgtctt tccctaccca ttgtggtcct
    97501 tcttctgcag tgacaccact tgcttctccg acagtcatga tatcgtactc ttcaaaaaca
    97561 tgtttcaaat cggtcaaatg ttcttcgatt cctgagacat tttgaaaggg tgagaaaggg
    97621 ttctctgtgg cttttactga atattcatct ttttttacat gagaaatcgc atctaaacga
    97681 aatccatcta tccctttatc caaccaccat cggatcatat tgaatagttc ttctttcaat
    97741 ttctcgcttt cccaatttaa gtctggttgt tcttttgcaa aaacatgcag ataatactgt
    97801 ccagtttcat gagaatactc ccaagcagaa ccgccgaaaa tcgatgtcca attattcggt
    97861 tctttgccat ctaccccgtc gacccaaatg taataatcgc gatacgggct ttctttagat
    97921 tttttcgatt cttcaaacca ttgatgctga tccgaactat ggttgatgac aagatccata
    97981 atcactttga tccctaattc atgtgcttct tttagcaata aatcaaaatc ttccatcgtt
    98041 ccgaatctct cttctatatc ttgatagtca cttacgtcat aaccattatc gacaaatgga
    98101 gattgataga tcggattgat ccagataaag ccgataccaa gatctttcaa ataattaagt
    98161 ttttcacgga tcccattgat gtctccttga ccatcgccat tgctatcttt gaaactagca
    98221 ggatagattt gatagcctac tgcttctttc caccattttt gttcttgtat catatcgttt
    98281 cactccttta acggtccctc ttgattataa ccgattctat tttagaagta tccttgttac
    98341 cggtaactag aaacgaaaag ctcaacgaat ctgctaagtg cgttcgttga gctttatttt
    98401 atcatagata gcgggcaatc agttgaatca tttgcttggg aaattcattc aagtcagtaa
    98461 tatctacaaa acgactttca ccgtagatcg acttgatttc ttctttgtct tggccaatag
    98521 ctgcggagac aaataggatt ccttgacgtt catattcttt caaaacagac tggatatctt
    98581 cttttgcttt tttgcctgta taatccggca atgcttttgg ctgaccgtca ctaatattaa
    98641 gcaacagttt ggttgttgcg gattgtctgt ttaatttttc tgctgctaaa cgaagtgcag
    98701 caccatcacg attgttttgc cttggtttca tagtgactaa tttttcattt aaccattgaa
    98761 atccgtcttc aaattcttta taagaaaaca aagaagtctt ttctcttgac gaacggtctg
    98821 ctgtatctcc gtaaatcaac aatgggatat cgactttctt agaaaattcg gcaatggcaa
    98881 tcgctgcttt ttttgccgct tcgatccgat catcccgtat cattgaccca gattcatcga
    98941 tccgtacagc taccgctaaa gaaggttgct catgtggcgg gttctttttg tcaaaatttc
    99001 tcaaatcacc atatgctact ttgcttgcat tgaaccgctg accttcgtat tttccttttt
    99061 ggtacgtctc tgtttgttca ttatccagta actctaaaat ttgccggcta agtgattcta
    99121 ctattggcat tacttgcatg tgtagctcac gtgcttccgg cgtttttcct tggaaagctg
    99181 gtcggtgaac gatgagacct tctttttcat gaatcgtttc cttcatgatt tgtcgcgctt
    99241 ctttattcag ttgcttcttg atttccttat catgtgcttc catgtcagaa gcagacattt
    99301 cttgagaatc atctcctgtt tgttgttctt tttgattttt tccttcgata tcgctactgc
    99361 gttctttttg catttgagga ggaatctcgt ttttttctat tgtttcttct tgtttctctt
    99421 ctccctcatt tggttcttca gcagacaacg atttcttttc atctaagcgc agaatttctt
    99481 cagcattttc agtgatgttt cgttcatttg cttcttcggc gctaatggtt ctttcttttg
    99541 tatgctcaga ttgaggatga ccccattttt cttttctcaa tatgtcctga agttccagta
    99601 gcaatccact gtcagacaaa gcttgttcaa gaattgccaa ttcatctgaa tcagtggata
    99661 atttatacaa aacttttgga taaatcgact ggagtaaatc ttgtccttgc ttataggtat
    99721 ttgcccaata aaaataagaa cgcattcctg ctacaccttt gatggcgtta gcttttgcag
    99781 tttcatccaa caaacggata atctctgcca tctttaagag aagttcttgt ttttggatca
    99841 tagttttcgc catcgcacgt tctgccatga cttctaaggc aggaagatcc attttttccg
    99901 catgttgcat ccgatctctt aaagattcat ttaatgggcg atttccttgg taatttcgat
    99961 ttgtagtgat cacaatgacg caatctggat ggcgacgaat gattcctgtc ggtaaattca
   100021 acatcccatt tggttcgagc gctgagttaa gtgccacaag aacagaggca tctcggatga
   100081 cagtcggctc ttgaatctct aataaatagc ctttttcaat cgctcgaacg atttcagaag
   100141 ggtaatagct gtaatggatt gcatctggat aatcagcttc gatcctttga actagctctg
   100201 ctaatttttc tttagcagaa aaaggatcaa tgccgtaatg gttctggatc agtgtcagta
   100261 cagcttcaag tgtatctgtt tggtaaatag cttctagtaa ttcttggtct tgttccttat
   100321 tagaatctac tacaggtaat aaggctccga aaacatctga tttgtccata tctgcaaaac
   100381 aggtcacttt tgtataggga agttgcagat cagcagatag tgctttggct aattgtgtct
   100441 tccccgaccc ggcatctcct tccaaaagta cgttactgat tttcatttca ggatcttgcc
   100501 aattcccttt gatttcggag gcgatccgcc attcttctgc acttgtccga tggcttttcg
   100561 gtttcttcca gatcatttgc ttttctagcc gtgaaaaggt tcggttttct gttagtgaga
   100621 aattctccat gcttctcttc cccttttgat catctctgtc tattaatatc ctagctcatc
   100681 taaaagacgt tttaaaatac ggaatcgttc cccaatcagt tctccgtctt tttgtagtgt
   100741 atcgtaatac aaaatgatgt cttcatcgat ctttggattt ttggtatcta cttctaccgt
   100801 cattcggttc ccttgtaatc caatgatgtc tacgactgga ttttctggat cataaaattt
   100861 ttcataacga taagcatctt ggaagtataa acttccttgc gcagctaaga tcgctaaatc
   100921 taaaatacgt gtgattggta attcttccga ctgtcgtgac catttttctc cagtatgtcg
   100981 ccataccttg cctgagatat cgacttttcc acgatcattc cactgagcaa ggcccagtga
   101041 aagccctttt gcatccatat ttaatgcatt tcttccatca actttatcat agtcttctac
   101101 aacgattact ggtttatgtt ttaaatttgt tggtatttcc attattttaa ctcctcatcc
   101161 ttttattttt agtaaataag taaattagta gtttactcat ttactaaact atatgcgaca
   101221 ttatagagga ttgaagtgag gattgcaaag aaaaagagct tcattgatag aaataaaaac
   101281 accatctcgt tcttttacac gctcattagt aacatgtaga aagtccaaga tgatgttgga
   101341 at
//
LOCUS       DACKAC010000002        98479 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.002, whole genome shotgun sequence.
ACCESSION   DACKAC010000002 DACKAC010000000
VERSION     DACKAC010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 98479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 98479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..98479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.002"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            591..1280
                     /locus_tag="I0B46_000092"
     CDS             591..1280
                     /locus_tag="I0B46_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAR2082426.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="I0B46_000093"
     CDS             1267..2475
                     /locus_tag="I0B46_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAR2082427.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="I0B46_000094"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="I0B46_000094"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAR2082428.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="I0B46_000095"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="I0B46_000095"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAR2082429.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="I0B46_000096"
     CDS             complement(6578..7273)
                     /locus_tag="I0B46_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAR2082430.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLTPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="I0B46_000097"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="I0B46_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAR2082431.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="I0B46_000098"
     CDS             complement(8472..8663)
                     /locus_tag="I0B46_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAR2082432.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="I0B46_000099"
     CDS             complement(8684..9574)
                     /locus_tag="I0B46_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAR2082433.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="I0B46_000100"
     CDS             complement(9561..10328)
                     /locus_tag="I0B46_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAR2082434.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="I0B46_000101"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="I0B46_000101"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAR2082435.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="I0B46_000102"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="I0B46_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAR2082436.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEVQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="I0B46_000103"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="I0B46_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAR2082437.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14944..15282
                     /locus_tag="I0B46_000104"
     CDS             14944..15282
                     /locus_tag="I0B46_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2082438.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(15450..16208)
                     /locus_tag="I0B46_000105"
     CDS             complement(15450..16208)
                     /locus_tag="I0B46_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAR2082439.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="I0B46_000106"
     CDS             complement(16226..17044)
                     /locus_tag="I0B46_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAR2082440.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="I0B46_000107"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="I0B46_000107"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAR2082441.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="I0B46_000108"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="I0B46_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAR2082442.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="I0B46_000109"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="I0B46_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAR2082443.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..20834
                     /gene="dnaN"
                     /locus_tag="I0B46_000110"
     CDS             19704..20834
                     /gene="dnaN"
                     /locus_tag="I0B46_000110"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAR2082444.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21063..21305
                     /gene="yaaA"
                     /locus_tag="I0B46_000111"
     CDS             21063..21305
                     /gene="yaaA"
                     /locus_tag="I0B46_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAR2082445.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21292..22416
                     /gene="recF"
                     /locus_tag="I0B46_000112"
     CDS             21292..22416
                     /gene="recF"
                     /locus_tag="I0B46_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAR2082446.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22413..24359
                     /gene="gyrB"
                     /locus_tag="I0B46_000113"
     CDS             22413..24359
                     /gene="gyrB"
                     /locus_tag="I0B46_000113"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAR2082447.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24379..26850
                     /gene="gyrA"
                     /locus_tag="I0B46_000114"
     CDS             24379..26850
                     /gene="gyrA"
                     /locus_tag="I0B46_000114"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAR2082448.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDRAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27086..27385
                     /locus_tag="I0B46_000115"
     CDS             27086..27385
                     /locus_tag="I0B46_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAR2082449.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27435..27947
                     /gene="ssb"
                     /locus_tag="I0B46_000116"
     CDS             27435..27947
                     /gene="ssb"
                     /locus_tag="I0B46_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAR2082450.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27973..28209
                     /gene="rpsR"
                     /locus_tag="I0B46_000117"
     CDS             27973..28209
                     /gene="rpsR"
                     /locus_tag="I0B46_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAR2082451.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28352..30325
                     /locus_tag="I0B46_000118"
     CDS             28352..30325
                     /locus_tag="I0B46_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAR2082452.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30331..30783
                     /gene="rplI"
                     /locus_tag="I0B46_000119"
     CDS             30331..30783
                     /gene="rplI"
                     /locus_tag="I0B46_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAR2082453.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31057..32424
                     /gene="dnaB"
                     /locus_tag="I0B46_000120"
     CDS             31057..32424
                     /gene="dnaB"
                     /locus_tag="I0B46_000120"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAR2082454.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNIIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33204..34496
                     /locus_tag="I0B46_000121"
     CDS             33204..34496
                     /locus_tag="I0B46_000121"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAR2082455.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            35283..36146
                     /locus_tag="I0B46_000122"
     CDS             35283..36146
                     /locus_tag="I0B46_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAR2082456.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(36275..37897)
                     /locus_tag="I0B46_000123"
     CDS             complement(36275..37897)
                     /locus_tag="I0B46_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAR2082457.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            38146..38667
                     /locus_tag="I0B46_000124"
     CDS             38146..38667
                     /locus_tag="I0B46_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2082458.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(38853..40583)
                     /gene="spxB"
                     /locus_tag="I0B46_000125"
     CDS             complement(38853..40583)
                     /gene="spxB"
                     /locus_tag="I0B46_000125"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAR2082459.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            40748..41566
                     /locus_tag="I0B46_000126"
     CDS             40748..41566
                     /locus_tag="I0B46_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAR2082460.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(41669..41866)
                     /locus_tag="I0B46_000127"
     CDS             complement(41669..41866)
                     /locus_tag="I0B46_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2082461.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            42035..42613
                     /locus_tag="I0B46_000128"
     CDS             42035..42613
                     /locus_tag="I0B46_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAR2082462.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            complement(43658..44161)
                     /locus_tag="I0B46_000129"
     CDS             complement(43658..44161)
                     /locus_tag="I0B46_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAR2082463.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            44519..45544
                     /locus_tag="I0B46_000130"
     CDS             44519..45544
                     /locus_tag="I0B46_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAR2082464.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            45867..46745
                     /locus_tag="I0B46_000131"
     CDS             45867..46745
                     /locus_tag="I0B46_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2082465.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            47221..47337
                     /locus_tag="I0B46_000132"
     CDS             47221..47337
                     /locus_tag="I0B46_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAR2082466.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            48264..48602
                     /locus_tag="I0B46_000133"
     CDS             48264..48602
                     /locus_tag="I0B46_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2082467.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            48696..48926
                     /locus_tag="I0B46_000134"
     CDS             48696..48926
                     /locus_tag="I0B46_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2082468.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            49326..49697
                     /locus_tag="I0B46_000135"
     CDS             49326..49697
                     /locus_tag="I0B46_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAR2082469.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(49971..50663)
                     /locus_tag="I0B46_000136"
     CDS             complement(49971..50663)
                     /locus_tag="I0B46_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAR2082470.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(50666..51451)
                     /locus_tag="I0B46_000137"
     CDS             complement(50666..51451)
                     /locus_tag="I0B46_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAR2082471.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            51688..53088
                     /gene="lacG"
                     /locus_tag="I0B46_000138"
     CDS             51688..53088
                     /gene="lacG"
                     /locus_tag="I0B46_000138"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAR2082472.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            53113..53430
                     /locus_tag="I0B46_000139"
     CDS             53113..53430
                     /locus_tag="I0B46_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAR2082473.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            53443..55116
                     /locus_tag="I0B46_000140"
     CDS             53443..55116
                     /locus_tag="I0B46_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAR2082474.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            55130..56761
                     /locus_tag="I0B46_000141"
     CDS             55130..56761
                     /locus_tag="I0B46_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAR2082475.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(57252..58817)
                     /gene="guaA"
                     /locus_tag="I0B46_000142"
     CDS             complement(57252..58817)
                     /gene="guaA"
                     /locus_tag="I0B46_000142"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAR2082476.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHDLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(58876..59799)
                     /locus_tag="I0B46_000143"
     CDS             complement(58876..59799)
                     /locus_tag="I0B46_000143"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAR2082477.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            59995..60600
                     /locus_tag="I0B46_000144"
     CDS             59995..60600
                     /locus_tag="I0B46_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAR2082478.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            60908..61924
                     /locus_tag="I0B46_000145"
     CDS             60908..61924
                     /locus_tag="I0B46_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAR2082479.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            62065..63366
                     /locus_tag="I0B46_000146"
     CDS             62065..63366
                     /locus_tag="I0B46_000146"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAR2082480.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            63412..64074
                     /gene="deoC"
                     /locus_tag="I0B46_000147"
     CDS             63412..64074
                     /gene="deoC"
                     /locus_tag="I0B46_000147"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAR2082481.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIVETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            64095..64493
                     /locus_tag="I0B46_000148"
     CDS             64095..64493
                     /locus_tag="I0B46_000148"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAR2082482.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            64559..65641
                     /locus_tag="I0B46_000149"
     CDS             64559..65641
                     /locus_tag="I0B46_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAR2082483.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            65853..67415
                     /locus_tag="I0B46_000150"
     CDS             65853..67415
                     /locus_tag="I0B46_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAR2082484.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            67408..68541
                     /locus_tag="I0B46_000151"
     CDS             67408..68541
                     /locus_tag="I0B46_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAR2082485.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            68541..69497
                     /locus_tag="I0B46_000152"
     CDS             68541..69497
                     /locus_tag="I0B46_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAR2082486.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            69596..70762
                     /gene="deoB"
                     /locus_tag="I0B46_000153"
     CDS             69596..70762
                     /gene="deoB"
                     /locus_tag="I0B46_000153"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAR2082487.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            70786..71604
                     /locus_tag="I0B46_000154"
     CDS             70786..71604
                     /locus_tag="I0B46_000154"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAR2082488.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            71621..72328
                     /gene="deoD"
                     /locus_tag="I0B46_000155"
     CDS             71621..72328
                     /gene="deoD"
                     /locus_tag="I0B46_000155"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAR2082489.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(72626..73312)
                     /gene="gpmA"
                     /locus_tag="I0B46_000156"
     CDS             complement(72626..73312)
                     /gene="gpmA"
                     /locus_tag="I0B46_000156"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAR2082490.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(73484..74065)
                     /locus_tag="I0B46_000157"
     CDS             complement(73484..74065)
                     /locus_tag="I0B46_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAR2082491.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(74198..74881)
                     /gene="rpiA"
                     /locus_tag="I0B46_000158"
     CDS             complement(74198..74881)
                     /gene="rpiA"
                     /locus_tag="I0B46_000158"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAR2082492.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISAGFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            75200..75613
                     /gene="rpsL"
                     /locus_tag="I0B46_000159"
     CDS             75200..75613
                     /gene="rpsL"
                     /locus_tag="I0B46_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAR2082493.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            75697..76167
                     /gene="rpsG"
                     /locus_tag="I0B46_000160"
     CDS             75697..76167
                     /gene="rpsG"
                     /locus_tag="I0B46_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAR2082494.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            76261..78348
                     /gene="fusA"
                     /locus_tag="I0B46_000161"
     CDS             76261..78348
                     /gene="fusA"
                     /locus_tag="I0B46_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAR2082495.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            78520..79707
                     /gene="tuf"
                     /locus_tag="I0B46_000162"
     CDS             78520..79707
                     /gene="tuf"
                     /locus_tag="I0B46_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAR2082496.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            80114..80422
                     /gene="rpsJ"
                     /locus_tag="I0B46_000163"
     CDS             80114..80422
                     /gene="rpsJ"
                     /locus_tag="I0B46_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003672692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAR2082497.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKDKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            80453..81082
                     /gene="rplC"
                     /locus_tag="I0B46_000164"
     CDS             80453..81082
                     /gene="rplC"
                     /locus_tag="I0B46_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAR2082498.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            81110..81733
                     /gene="rplD"
                     /locus_tag="I0B46_000165"
     CDS             81110..81733
                     /gene="rplD"
                     /locus_tag="I0B46_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAR2082499.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            81733..82023
                     /gene="rplW"
                     /locus_tag="I0B46_000166"
     CDS             81733..82023
                     /gene="rplW"
                     /locus_tag="I0B46_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAR2082500.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            82062..82895
                     /gene="rplB"
                     /locus_tag="I0B46_000167"
     CDS             82062..82895
                     /gene="rplB"
                     /locus_tag="I0B46_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAR2082501.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            82938..83216
                     /gene="rpsS"
                     /locus_tag="I0B46_000168"
     CDS             82938..83216
                     /gene="rpsS"
                     /locus_tag="I0B46_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAR2082502.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            83239..83586
                     /gene="rplV"
                     /locus_tag="I0B46_000169"
     CDS             83239..83586
                     /gene="rplV"
                     /locus_tag="I0B46_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAR2082503.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            83600..84256
                     /gene="rpsC"
                     /locus_tag="I0B46_000170"
     CDS             83600..84256
                     /gene="rpsC"
                     /locus_tag="I0B46_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAR2082504.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            84259..84693
                     /gene="rplP"
                     /locus_tag="I0B46_000171"
     CDS             84259..84693
                     /gene="rplP"
                     /locus_tag="I0B46_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAR2082505.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            84683..84871
                     /gene="rpmC"
                     /locus_tag="I0B46_000172"
     CDS             84683..84871
                     /gene="rpmC"
                     /locus_tag="I0B46_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAR2082506.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            84896..85162
                     /gene="rpsQ"
                     /locus_tag="I0B46_000173"
     CDS             84896..85162
                     /gene="rpsQ"
                     /locus_tag="I0B46_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAR2082507.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            85220..85588
                     /gene="rplN"
                     /locus_tag="I0B46_000174"
     CDS             85220..85588
                     /gene="rplN"
                     /locus_tag="I0B46_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAR2082508.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            85625..85933
                     /gene="rplX"
                     /locus_tag="I0B46_000175"
     CDS             85625..85933
                     /gene="rplX"
                     /locus_tag="I0B46_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAR2082509.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            85961..86500
                     /gene="rplE"
                     /locus_tag="I0B46_000176"
     CDS             85961..86500
                     /gene="rplE"
                     /locus_tag="I0B46_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAR2082510.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            86519..86704
                     /locus_tag="I0B46_000177"
     CDS             86519..86704
                     /locus_tag="I0B46_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAR2082511.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            86740..87138
                     /gene="rpsH"
                     /locus_tag="I0B46_000178"
     CDS             86740..87138
                     /gene="rpsH"
                     /locus_tag="I0B46_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAR2082512.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            87170..87706
                     /gene="rplF"
                     /locus_tag="I0B46_000179"
     CDS             87170..87706
                     /gene="rplF"
                     /locus_tag="I0B46_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAR2082513.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            87867..88223
                     /gene="rplR"
                     /locus_tag="I0B46_000180"
     CDS             87867..88223
                     /gene="rplR"
                     /locus_tag="I0B46_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAR2082514.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            88244..88744
                     /gene="rpsE"
                     /locus_tag="I0B46_000181"
     CDS             88244..88744
                     /gene="rpsE"
                     /locus_tag="I0B46_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAR2082515.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            88759..88938
                     /gene="rpmD"
                     /locus_tag="I0B46_000182"
     CDS             88759..88938
                     /gene="rpmD"
                     /locus_tag="I0B46_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAR2082516.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            88982..89422
                     /gene="rplO"
                     /locus_tag="I0B46_000183"
     CDS             88982..89422
                     /gene="rplO"
                     /locus_tag="I0B46_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAR2082517.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            89422..90717
                     /gene="secY"
                     /locus_tag="I0B46_000184"
     CDS             89422..90717
                     /gene="secY"
                     /locus_tag="I0B46_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAR2082518.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            90779..91426
                     /locus_tag="I0B46_000185"
     CDS             90779..91426
                     /locus_tag="I0B46_000185"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAR2082519.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            91617..91835
                     /gene="infA"
                     /locus_tag="I0B46_000186"
     CDS             91617..91835
                     /gene="infA"
                     /locus_tag="I0B46_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAR2082520.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            91868..91984
                     /gene="rpmJ"
                     /locus_tag="I0B46_000187"
     CDS             91868..91984
                     /gene="rpmJ"
                     /locus_tag="I0B46_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAR2082521.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            92002..92367
                     /gene="rpsM"
                     /locus_tag="I0B46_000188"
     CDS             92002..92367
                     /gene="rpsM"
                     /locus_tag="I0B46_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAR2082522.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            92395..92784
                     /gene="rpsK"
                     /locus_tag="I0B46_000189"
     CDS             92395..92784
                     /gene="rpsK"
                     /locus_tag="I0B46_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAR2082523.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            92864..93802
                     /locus_tag="I0B46_000190"
     CDS             92864..93802
                     /locus_tag="I0B46_000190"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAR2082524.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            93830..94210
                     /gene="rplQ"
                     /locus_tag="I0B46_000191"
     CDS             93830..94210
                     /gene="rplQ"
                     /locus_tag="I0B46_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAR2082525.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            94482..95321
                     /locus_tag="I0B46_000192"
     CDS             94482..95321
                     /locus_tag="I0B46_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAR2082526.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            95297..96166
                     /locus_tag="I0B46_000193"
     CDS             95297..96166
                     /locus_tag="I0B46_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAR2082527.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            96156..96953
                     /locus_tag="I0B46_000194"
     CDS             96156..96953
                     /locus_tag="I0B46_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAR2082528.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            97156..97902
                     /gene="truA"
                     /locus_tag="I0B46_000195"
     CDS             97156..97902
                     /gene="truA"
                     /locus_tag="I0B46_000195"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAR2082529.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
BASE COUNT        30777 a        18157 c        20435 g        29110 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggttgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tgtattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtgtcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga cttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa agaaatagaa
    20881 aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg tttatttttg
    20941 tttaaatcac cttttttaga ttaaatcgtc ttatatggaa aaatatatgg ataaatttgt
    21001 tttattgcga aaaaaaaggt ataatagagt atacttttaa taatgaaaat gagggatggg
    21061 cattgaaaca acaagtcatt ctaaatacgg aattcatgac gttgggccaa atgttgaaag
    21121 aagtcacagt tatcggtagt ggcggacaag caaaatggta tttggcagaa aacactgtgt
    21181 tagtcgatgg cgaaccagaa aatcgtcgtg gacgcaaact ttatcccggc atgatggttg
    21241 aagttccaga agtaggaact ttttttatgg taaaaaaagg aaacgacgat aatgaggctg
    21301 aataagctgt atttaaaaaa ttatcgaaat tacgaagagc tgaacatcga attttccaag
    21361 aacctcgtca tctttttagg agaaaatgcg caaggaaaaa cgaatctttt ggaaagtatc
    21421 tacgtattag ctatgacgcg aagtcatcgg acaagtagtg agcaggaatt gattggctgg
    21481 actgacgatc aggcgatgat tcaaggagag atcacaaaag gttcatccac tcttccatta
    21541 gaaatcttat tatctaaaaa aggccgaaaa accaagatca accatatcga acagaaaaag
    21601 ttaagtactt acgtgggaca aatgaatgtc attttatttg caccagaaga tctttcttta
    21661 gtaaaaggca gcccgcagat caggcggaag tttttagata tggaattagg ccagatcaat
    21721 ccaatctatc tttatgatct tgtccaatac caagcaattt tgaaacaaag aaaccagtac
    21781 ttgaagcaat tgaatgaaaa aaaacaaact gatttgcttt atttggatat tttgtccgaa
    21841 caactagcag cgtttggcag caaagtattg aaagcaagag cacagttcgt caagcgattg
    21901 gagttttggg ctaattcttt gcatcagcaa atcacccatc aaaaagagca gctggaaatc
    21961 gagtatctca ccgcagtcga ttctttagaa acacatacgc aagagcaaat ccaagaacaa
    22021 tttctcgcac ttttgaacca aaataaaaag aaagaccttt ttcggggaac aacgacagtt
    22081 ggtcctcatc gtgacgatct ttcctttttt attaatcaga aaaatgtgca gacttatgga
    22141 tcgcaaggac agcaaagaac aactgcacta agtgtcaaat tagcagaaat cgatttgatt
    22201 aaagaagaga caggggaata tccaatcctt ttgttagatg atgtgatgag cgagctggat
    22261 gataatcgtc aactccatct gctagaaaca atcgaaggaa aagttcaaac gttcttaaca
    22321 acaacgacac ttgaccatgt aaaaaataaa atgactgttg aacctgagat tttttatgtt
    22381 caacaaggga aactaaaagg agaagaaact gaatgacaga agaaagaagt ttagtagaac
    22441 gcgctaaaga gtatgatgcc agtcagattc aagtacttga aggacttgaa gccgttcgta
    22501 aacgacctgg catgtatatc ggttcgacta gttctgaggg tctgcatcac cttgtttggg
    22561 aaattgtcga taattcgatc gatgaagtat tagcaggttt tgcaacgaaa atccatgtga
    22621 ttattgaaaa agacaacagt atcaccgtta ttgacgacgg tcgggggata cctgtagata
    22681 tccaggcaaa aactggacgt cctgcagttg aaacggtctt tactgtttta catgctggag
    22741 gaaaattcgg cggtggtggg tacaaagttt ctggtggact tcacggagta ggatcttctg
    22801 ttgtaaacgc gctttctaca cagcttgatg tcaaagtata caaagaagga aatgtgtact
    22861 atcaagaata tcgtcgtgga gctgtagtgg acgacttaaa gattatcgaa caaacggatc
    22921 gccatggaac aacggttcac tttacgccag accctgaaat ttttacagag acaactgagt
    22981 ttgatttttc aaaattggct acccggatcc gtgaacttgc tttcttgaac cgcgggatgc
    23041 gtatatcgat tgaagacaaa cgagaagaag aaccagtcat caatgaatac cattatgacg
    23101 gcgggatcaa aagttatgtc gaatacttga atgcaaacaa aaccgtgatt tttccagaac
    23161 ctgtatattt ggaaggtgaa cagcaagata tcgcagttga agtctctatg caatatacag
    23221 acggctacca ttccaatatc atgagttttg ccaacaatat ccatacgtat gaaggtggaa
    23281 cacatgaatc aggattcaag actgctttaa ctcgtgtgat aaatgattat gcccgaaaac
    23341 aaaagctgat gaaagaaaat gacgacaact tgaccggtga agacgtgcga gaaggattga
    23401 cagctgttat ttctatcaag catcctgacc ctcaattcga aggacaaaca aaaacaaaat
    23461 tgggcaattc cgaagtacgg acagtcactg atcggttatt tagcgaacat ttcatgaaat
    23521 tcttgctgga aaacccaagt gtgggccggc aaattgttga aaaaggatta ctagcttcca
    23581 gagctcgtct agcagcgaaa cgtgcgcgtg aagtaacgag acgtaaaggt gctttagaga
    23641 ttagtaatct gccaggaaaa ttagcggact gctctagcaa cgatcctgaa aaatgcgaat
    23701 tgttcatcgt cgaaggagat tcggccggcg gatcagctaa gcaaggtcgt aatcgagagt
    23761 ttcaagccat cttgcctatt cgtgggaaaa tcttgaatgt tgaaaaagca agcatggaca
    23821 agatcttggc aaatgaagag atccggtctt tatttactgc tatgggtaca gggtttggag
    23881 aagattttga tgtatcgaaa gctcgctacc acaaattagt tatcatgact gatgccgatg
    23941 tcgacggcgc gcatatccgt acattgctgc tgactctttt ttatcgttac atgcgtccta
    24001 ttgttgaagc tggatacgtc tatattgctc agccaccttt atacggggta aaacaaggga
    24061 agaatattac gtatgtacaa ccaggaaaaa atgcagaaga agagttgaaa caagtcgttg
    24121 cttcacttcc tgcctcacca aaaccaagcg ttcagcgtta taaaggactt ggagaaatgg
    24181 atgatcatca actatgggaa acaactatgg acccaagcaa tcggatgatg gcccgcgtca
    24241 gtgtggacga tgctattgca gcagatcaga tatttgaaat gttgatgggg gatcgagtag
    24301 aaccccgtcg tgcattcatc gaggaaaatg cacactatgt gaagaactta gatatttaat
    24361 catgaaggag gaaaaagaat gagtgaagaa atcagagaaa acatccatga tgtcaatcta
    24421 accagcgaaa tgaaagactc cttcatcgat tatgcgatga gtgttattgt cgcacgtgca
    24481 cttcctgatg tcagagatgg tctgaaaccc gttcatcgcc gtattttata cggcatgaac
    24541 gaattaggtg taacaccgga taaagcccat aaaaaatctg ccagaatcgt cggagatgtc
    24601 atgggtaaat accatcctca tggagaccgt gcgatctatg agtcaatggt acggatggca
    24661 cagccattca gctaccgtta tatgctagtg gatggtcatg gaaactttgg ttctgttgac
    24721 ggtgacggtg ccgccgctat gcgttatacc gaagcacgga tgagcaaaat cgctacagaa
    24781 atgctgcgtg atatcaataa gaacactgtt gatttccaaa gcaactatga tgatacagaa
    24841 aaagaaccag ttgtactacc tgctcgcttc ccgaatcttt tagtgaacgg aacaacgggg
    24901 atcgccgtag gtatggccac gaatatccca cctcataact tgtctgaggt agtagcggct
    24961 atcgatttgc tgatggataa tcctgatgtg acgaccaatg aattgatgga agtattacct
    25021 ggaccagatt ttccaacagg tggtttggtt atggggaaat caggaatccg aagagcgtat
    25081 gaaacaggta agggttctat taccgtacgt gcaaaagttg agattactga gatgccaaat
    25141 ggtaaagaac ggatcatcgt gacagaattg ccttatatgg tcaataaagc aaaattgatc
    25201 gaacggattt ctgaactgca cagagacaag cgtatcgaag ggatcacaga tttgcgggac
    25261 gaatcttccc gtgaaggtat gcggatcgtc attgacatcc gtcgtgacgc cagcgcatcc
    25321 gttatcttga ataacttata taaattgaca tccttgcaaa attcatttgg attcaatatg
    25381 ttggcgatcg aaaaaggtgt gccgaaagtt ttgagcttga aacaaatcct tgaaaactat
    25441 atcgaacatc aacgcgaagt catcacacga cggacggtct ttgaaaaaga aaaagcagaa
    25501 gcaagagcac acatcttaga aggtttgcgt attgctttgg atcatatcga tgaaatcatt
    25561 gcgattatcc gcgggtctaa atcagatgac gaggcaaaag caacaatgat cgaacggttc
    25621 gatttatccg atcgccagtc tcaagctatt ttagatatgc gtttacgccg attgaccggt
    25681 ttggaacggg aaaaaatcga aaatgaatac caagaactct taaaactgat tgaagattta
    25741 actgacattc tggcaagacc agaacgtgtt acagaaatca tcaagaatga attagcagac
    25801 ttgaatcaac gatttggtga taaacgacgc acagaattac tagtcggtga ggtcttaagt
    25861 ctggaagatg aagatttaat cgaagaagaa gaaatcgtta ttacattgac aaacaatggt
    25921 tacatcaaac gtttggcaaa taatgaattc agagctcaac gaaggggtgg acgtggtgtc
    25981 caaggaatgg gtatccacaa tgacgacttc gtcaaaaatc tggtttcttg ttctacccat
    26041 gatacattgt tattctttac gaataacgga aaagtgtatc gggcaaaagg atacgaaatt
    26101 ccagaatacg ggcgtacagc caaaggaatc ccaatcatca atttactagg tatcgattcg
    26161 agtgaaagca tccaggcaat cattgcggtg acgggagaag cagaagaagg ccactatctc
    26221 ttctttacga cgcgtcaagg tacagtaaaa cgtacttctg tcactgcttt tgctaacatc
    26281 cgaagtaatg gtctgatcgc aattggtttg aaagaagacg atgagctcgt caatgtgcta
    26341 ctgacagatg gtcaatctaa tatcattatt ggaacacata acggctactc tgtcactttc
    26401 aaagaagaag cagtccgcga tatgggacgt acagcttccg gggtcagagg gatacgcctg
    26461 cgtgaggaag attatgtcgt tggggcagcc ttaatgaagg aaaatcaaga agtattagtg
    26521 atcacagaaa aaggttacgg aaaacggaca aaagtatcgg aatatccagt gaaaggccgc
    26581 ggaggtaaag ggatcaaaac ggcgaacatc actgaaaaaa atggtccact agctggattg
    26641 accactgttt ctggagatga agacatcatg ctgattacag ataaaggtgt cattatccgt
    26701 ttcaacgtat cgactgtttc tcaaactgga cgttcaacat taggcgtacg tttgatgaag
    26761 atggaagctg atacgaaagt tgtcacgatg gcagcagtcg aaccagaagc tgcagaacaa
    26821 acagttgaag aacaaggatc agaagaataa ccatcaagac taagtgtgta agctagcaag
    26881 aataaccgat taaaaaatct ggttattcct gctggctttt ttgattagtt gaatttctct
    26941 taaaatattc gaaactgttc attctgtatt gcttttgacc aaaatagagt gtataattag
    27001 agcttgtgag tagtctaatt aattagataa ctctccttgc tcttaggaac ctaagagccg
    27061 aaagtccaca gggaggtgaa tatcaatgga aaacacgaaa tatgaaatca tgtacattat
    27121 tcgtccaaac attgatgaag aagcaaaaac tgctttaatc gaacgtttcg atgcaatctt
    27181 gaaagataac ggagcagaag ttatcgaatc aaaagattgg gaaaaacgcc gtttcgctta
    27241 tgaaatgaac ggttaccgtg aaggtatcta tcacatcgtt aaagtatctt ctccatcatc
    27301 agcaaatgct gtcaatgaat ttgaccgtct tgctaaaatc aacgacgata tcattcgtca
    27361 catgattgtt aaagaagaag cttaattgtt gtttcatgtg aaacaataga aaacgatgag
    27421 aggagatgac tattttgatt aataatgttg tattagttgg acgtctgacg aaagatccag
    27481 atttgcgtta cacagcaagt ggtacggcag tcgcaacatt tactttggct gtgaatcgta
    27541 attttacaaa ccaaagcggc aatcgcgagg ctgattttat caactgtgtg atttggcgca
    27601 aatcagcaga aactttagca aactatgctc gtaaaggaac acttttaggt gttactggac
    27661 gtatccaaac tcgttcttat gataatcagc aaggacaacg tgtatacgtg acagaagtag
    27721 tagctgacaa cttccaattg ctggaaagtc gttctgcatc tgaaaatcgc caacaaagcg
    27781 gcggattcca aagttctggt caatcagctg gcggatttgg cggaaacaat aactctaatc
    27841 aaacatctca atcatccaac ggtatgcctg atttcgaccg tgatacttct gacccattcg
    27901 gctcaagttc aacgatcgac atttcagacg atgatttacc attctaaacg aatttgatag
    27961 gagggaaata gaatggcaca acaaagaaga ggcggacgta aacgtcgtaa agtagactac
    28021 attgctgcta accatattga atatatcgat tataaagata tcgaattact aaaaagattt
    28081 atttcagaac gcggtaaaat cttgccacgt cgtgtaactg gtacaggcgc taaaaaccaa
    28141 cgtaaaatta ctgttgcaat caaacgcgct cgtatcatgg gcttgctacc tttcgttagc
    28201 gaagactaat aaatcctagg aattgaatga ctgatccttt tgatcagcta ttcaattcct
    28261 ttttttctgt aaaaataacc tctattcgtt tagtcgctcg aatgacgaaa gttatggtaa
    28321 aatcaaatta gtcatatatg ggaggaaaat tatggaaaat aaaaacaatc atttatcccc
    28381 gaaacctctc ttccttatcc tgtttatatt aatcgaagta ttggtagtct taattttacc
    28441 atttcgtttt gttagcgctt cggtcattct attgatgaat ttatggggtg ttcgctattg
    28501 gttgaaaatg aaacgttggc ttattttaaa taaacgtgaa aaaatccagc aggcttcgac
    28561 tgtagctgaa accaatctag cttatgtctc agaagtcatg cctgtaggag tgattgctta
    28621 tcagccatca agccaaaaaa tcgaatggct caatccatta gcattgaaaa tcaaagatca
    28681 aagtggatta acagaggaag aattgctaga tacttttttt actgcaaaag aaaaaaagaa
    28741 aaaccaagtc tatctagcag ataaaacatt tcagtttgat atggatacag taaagggtgt
    28801 tgtttatttt aatgatatta cccaaaacca gttgttaaaa ctccatcaga cgaacagtca
    28861 gccggtgatt ggtattttat ctattgataa ctatgcagat gccattgata aattagatga
    28921 caaagagatt tcttatttga actctttcgt cactacacta gtttctgact ggatgaatga
    28981 ataccatgtc tttttcaaaa gaatcaacgc agaacggttt ttcttcgtag cacagatgat
    29041 cgatctgaaa aagatgatgg ataaaaaatt tgatttgctg gatcgctttc gagcagaagc
    29101 tgaaaaacag agctcgccta tcacccttag tatggggatt gcttatggct caagaaccct
    29161 cgatgaaatc ggtggagaag ctcagaataa tttagatgtt gctttagtcc gtggaggcga
    29221 ccaagttgtc gtgaaagagg taaaagaagg agcaaaacct gtttattatg gggggaaatc
    29281 tgctaagaat gtcaaacgca cgcgtgttcg ttctcgagcg atgagtaccg ctttgcgtcg
    29341 gatttttgat gaaacaggcg atgtgtttat catggggcac aagtttccgg atatggatgc
    29401 tttaggctct gcttttggtg tcagttgtct ggcacgcttc aaccagaaaa aagcttacat
    29461 cgttatcaat gagaacgaga tcattccaga tgtagaacgg tgtcttgaag aaatccataa
    29521 gcatcctgaa ttagaaatgc aactaatctc tccagaacgt gccatagagc tgaaaaaaga
    29581 agaggacctc ctcgtgatgg tggattacca taaaccttct ctatcgatct ctcagggcct
    29641 atatgagcaa tttgataaga ttgtgatcat tgatcaccat cgtcgtgggg aagagtttcc
    29701 aagtaaacct ctgctgactt atattgaatc ttcagcttct tcagcttcag agctagtttc
    29761 tgagctgatc caataccaaa gcagcaaaac aaatcgctta gatcgttttg aggccacatt
    29821 attgcttgca gggatggttg tagatacaaa tagtttcaat gtccgtacaa ctgcacgaac
    29881 ttttgacgtt gccagctatt taaggtcttg tggagcagac gcatccttag tacaatattt
    29941 attgagctcg gatttagcga catacttgga gatcagcaat ttgattgctg gaagcgacta
    30001 tgtgacttct gatattgttg tggcggcagg gacagacgaa aaagagtata atagtgttat
    30061 agcagcaaaa acagcagata cattgctttc gatgatcaat atcaatgcag ctttcgtcat
    30121 tacaaagcgg acagacggct tgattgggat cagtgccaga agtaccggca cgatcaacgt
    30181 ccaaatcatc atggaatcct taggtggcgg aggacacttc acaaacgcag ctactcaact
    30241 ggaaaatacg acagttgagg aaacaaaaca gaagctattt gctgcaatca aagaaaatat
    30301 gaatcaaata tatgataagg agtaatccaa atgaaagtaa tttttttaca agatgtaaaa
    30361 ggaaaaggta aaaaagggga catcaaagaa gttccagcag gttatgcaca aaattatcta
    30421 atcaaaaata acttggcaaa agaagcaacg aaaagcagta tcagtcagtt agcaggacaa
    30481 aagaaagcac aagaaaaaca cgaagctgaa atcttggaag aagctaaaaa gttaaaagaa
    30541 ttcttggaaa aagaagatac tgtagtcgaa ttgaaggcga aagcagggga agacagccgt
    30601 ttgtttggtt cgatcccttc aaaacaaatt gctgaagggt taaacaaaca atacaaaatc
    30661 aaattagata aacgaaaaat cgaattagaa aatccgatcc gttcactagg ctacacaaac
    30721 gtacctgtta agcttcatca tgaggtgacg gctacgatca aagttcatgt cgtacaagaa
    30781 taacaaatct tgatagcgat gttcagctgg taagtataac cagaaggaaa aaagacaaac
    30841 cgaaatttat ctgagaaaac agctgcagca atgaaagcaa aagctgcaaa tcgatttcag
    30901 attgatttcg gtttcttttt tgggaacagc aaaatagcat aaagaaaacg agtataataa
    30961 aagagaacta tttttttatt acactttcgg tacgaatgat gacttttctc tcattttctt
    31021 gtaaaatgaa gcaattgaag acaaggagcg atacgtatga atgaagtgtg gcaggatcga
    31081 atcccaccgc aaaatattga agctgaacaa gcggttttag gctctgtttt tcttgacgca
    31141 gaaacgatca ttgatgccat ggagtacata gagccaaaag acttttatcg tcgtggccat
    31201 caaattattt ttgagacaat gatcgagtta aatgatcgta atgaagcgat tgacgtggtt
    31261 actgtcaaag accggctgga acaagccaat ttgttagaag atgccggtgg tttaagttat
    31321 ctctcagact tggcattagc agttccgaca gcagcaaata tcgtctacta tgcgaaaatc
    31381 gttgaagaaa aatcattgct gcgtacattg atccagacag caacaggaat cgttacgaaa
    31441 ggattcgagc aaggagaaga agttcaaaca attttagatg atgccgaacg cagtatcttg
    31501 gaggtttcgg aaaaaagaaa tcgaagcggc tttttatcta ttgctgaagt actgaactcc
    31561 acgattgaaa atatcgatcg actagctcaa aacaatgaag aaatcacagg tctaccaact
    31621 ggctatcaag gtctggataa aatgacagca ggtttccagc ccgaagaact aatcattctt
    31681 gctgcacgtc cagcggtagg taaaacagcg tttgcattga atattgctca aaatgtagga
    31741 acaaaaacag atcgttctgt tgcgatattt agtttggaaa tgggggcaga atctcttgta
    31801 aatcggatgc tttgtgcaga aggttcgatt gaggccagcc atttacgtac aggtcagctt
    31861 tcggaagaag aatggtcgaa tctgatcgtt gcgatgggga gtctttctcg agcaaatatt
    31921 tttatcgatg atacaccagg gatcaaaatc tctgaaatcc gtgcgaaatg tcggaaacta
    31981 gcgcaagaaa aaggcaatct aggcttgata ttgatcgatt atttgcagtt gattgaaggt
    32041 aacggtaaag agaaccgtca acaagaagta tcagatatct ctcgccaact gaaaaaactg
    32101 gcgaaagagc tgaaagtacc tgtcattgct ctttcacagt tatctcgtgg agtagaacag
    32161 cgacaagaca aacgaccagt gctaagtgat attcgtgaat ccggatcaat tgaacaagat
    32221 gcggacatcg tagccttctt gtatcgagat gattattatg aaagagaagg cggagatgac
    32281 gatgatggaa acgagccaaa aaatgacaac atcatcgaag tcatcatcga gaagaaccga
    32341 agcggtgcac gaggaacagt tgaactactg tttatcaaag aatacaataa gttttcgtcg
    32401 ctttcgccaa gagaagaatt ttaaagaaga ggttgtgagc cttgagcaat cccgagagct
    32461 ctcgtaagaa gatgcaagga gtaaccgcag gaacgactag agtacttaat ttaaacagat
    32521 ctccaaaggg attgtgacaa aagtcttgtc acagtccctt tttccgaata aacggtgttc
    32581 aatcagctga cccttggtat tagctcagca aacgtcaaaa tatgaggaac tattttgacg
    32641 tttgtttctc taatactcag gtcaaaacgc tgatcttacg acctcttttc cttaaacggt
    32701 gttctcctgc caactcctcg gtcacaagtc acaatatcat caaacatgag aagcagtttt
    32761 ttgaaattgc tccttgtgag tcagagttaa acggcaggct cacaacctct ttcttaaacg
    32821 gtgttcaaaa gcttgcatct gcggtaatac gcccaatcaa acgaaaaata tgagaagcta
    32881 tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac ctctgattca
    32941 cggtgttcca aaagtagctt cttcgaaaaa taagtcgaaa tttcacaaaa atttgagaag
    33001 caattttcgt acattccttc ttatttcttg aagctgacca cttctgttac aacctctttt
    33061 ttaataacca ttctaaaaag ataaagttcg ttttttaagc gtttttataa aataaaaata
    33121 aaaatcattc gattttattt gaaatatgta tctattattg atacaatggt tcaggattaa
    33181 ataatataga acgaggtgtt cacatgtcat ctgtagtagt agtaggtacg caatggggcg
    33241 atgaagggaa aggaaaaatc acagactttc tcagtgaaaa tgctgaagtg attgcacggt
    33301 accaaggtgg cgacaatgca gggcacacaa ttaagtttaa cggtgtgact tataaactgc
    33361 atttgattcc ttctgggatt ttttataaag acaagatcag tgtgatcgga aacggcgttg
    33421 tagtcaatcc taaatcattg gttaaagaat tagcttattt acatgaaaaa ggtgtagaaa
    33481 caagtaattt gcgtatttct gaccgcgcgc acgtaatctt gccttatcat atcaaattgg
    33541 accaattaca agaagatgct aagggcgaaa ataagattgg tacaacgatt aaaggaatcg
    33601 gacctgccta tatggataaa gcagctcgtg taggtatccg tattgcagac ttattagata
    33661 aagaaatttt tgaagaacgt cttcgtttga atctagatga caaaaaccgt caatttgtaa
    33721 aaatgtttga ttcagaacca cttgattttg acgatatttt tgaagaatat tatgaatacg
    33781 gacaacaaat caaacaatac gtcacagata cttccgttat cttgaatgat gctttggatg
    33841 caggcaaacg cgtgttgttt gaaggcgcac aaggtgtaat gctggatatc gaccagggaa
    33901 cttatccttt tgttacttct tctaacccag tagccggtgg ggtaacaatc ggaagcggtg
    33961 tagggccttc aaaaatcaat aaagttgtcg gcgtctgcaa agcctatacg tcacgtgtcg
    34021 gtgatggacc atttccaact gaattatttg atgaaacagg agaacaaatc cgcgaagtag
    34081 gacgagaata cggtacgaca actggtcgtc cgcgtcgtgt aggctggttt gattctgtag
    34141 ttatgcgcca ttctaaacga gtttcaggta ttaccaacct atctttaaac tcgatcgatg
    34201 tattaagtgg attggaaaca gtaaaaattt gtacggctta tgaattagat ggcgaactaa
    34261 tctaccacta tccagcaagc ttgaaagagt tgaaccgctg caaaccaatc tatgaagaat
    34321 tgccaggctg gtcagaagac attacaggat gcaaaacact tgcagaactg ccagaaaacg
    34381 cacgtaacta tgtacgtcgt atctcagagc ttgttggtgt acgtatctca acattttctg
    34441 tcggacctga tagaacacaa acaaacatct tagaaagcgt ctgggcgcaa atctaaggat
    34501 aaaaacgacg actgaatcag gacgttctta ccagagcgag tcgaagtgtt tatctatacg
    34561 ataataataa cttgaagagg tagtgaaaga gacgtttagc cttattgccg ataggctgtc
    34621 tcttgaacac tgtttattca gaataaggga ctgtgacaaa aaccgtgtca cagtcccttt
    34681 ttccgaacaa acggtgttca aaagcttgca tctacggcac taagcccaat cagatgaaaa
    34741 atatggagtg ctatttttat ctgattgttc ttactgcttg atgcagagca gctttttcac
    34801 aacctctcca ctaaacggtg ttctcctgcc aactcctcgg tcacaagtca caatattcaa
    34861 caaacatgag aagctgtttt ttgaaattgt tccttgtgac tcggagctaa acggcaggct
    34921 cacaacctct tggtaaacgg tgttcaatca gctgaccctc ggtattagct caaaaaacgg
    34981 caaaatatga ggaactattt tttccgtttt tttctctaat actcaggtca aaacgctgat
    35041 ttcacaacct ctgattcacg gtgttccaga agtagcttct acggaaataa gccgaaattt
    35101 cacaaaaatt tgagaagcaa ttttcgtaaa ttccttctta tttcttgaag ctgaccactt
    35161 ctgtcacaac ctcttatttt gattagaact tgcgaaaata aaccggttaa agtgtgtcta
    35221 gcgcaagaga tttgctataa tagacgataa gggtaaaatg taacgagaag gggttgaaaa
    35281 atgtgtatca aattgtaaca gattcttgtt gtgatctgcc ataccatgta ttagcaaaag
    35341 aaaaagttga ttttttatca atgaatctcc aattgaatga tcaagagtta gtagatgacc
    35401 tcggaaagac attcaattat gaccgctaca tgaatcagct gaaaaatggt gcgatgccta
    35461 cgacatcaca aatcaatgtt ggccgatatt tagaattttt ccgttcttat gcggaaaaac
    35521 agattcctgt tttatatgtg gctttttcca gtggattaag cggctcttac agtagtgcgc
    35581 ttcaagcggt tgaattgctg aaggaagaat atgaagaccc tgagatctat gtttttgata
    35641 caaaagctgc aagtattggg gaagggcttt tagtattgga agctgtaaga ttgaaaaaag
    35701 aaggtaaaag catcgaagaa accctagagt ggctggaaag taattatttg aaagtccatt
    35761 catgggtaac agtagacgat ctgaaatatc tagaacgagg cggtaggatt tccaaagcaa
    35821 gtgctactct tggcagtcta ttgagtgtaa agcctattat tacggtggat aaagaaggaa
    35881 ggctgacgaa tgttgacaaa gttcgaggaa gaaacaagtc gatccagaca atcgtcaatg
    35941 agacggtaaa aacaatcgtt gcaccacttg atcaaatcgt ttatgtggct tacgctgggg
    36001 atctagagag cgcggagaag gcaaaacggc ttttggaaga gcagataaag atgaaagatg
    36061 tgaagcttta tcccttagga ccaacgatcg ccagtcacac aggctatggc tgtatcgcga
    36121 ttttttcgat gggaatcagt cgataaaagc agtaactcat tctgtaaaaa agcccagaaa
    36181 ttgattgtaa caaaaacagc gactcttaaa agatagatga gcatcctatc tcttagaggt
    36241 cgctgttttt ttatttttta agctaaatag gccattatgc ccataactcg ttcaattgtt
    36301 tttgaatatc tgcattttcc aaaaattcat catatgtcgt atcagctcga tcgatcacac
    36361 ctttatccga aacggcaatg atgcggtttg ccaatgtttg gataaattga tggtcatgtg
    36421 acgcaaataa gattgagcca gtaaatgcca tcaagccgtc attcaatgct gtgattgatt
    36481 ctaagtctaa atggttcgtt ggatcatcta atacaagtac gttagctttc gaaagcatca
    36541 tttttgaaag catgcaacgg actttctctc caccagataa aacattcact ggtttcaata
    36601 cttcttcacc agaaaacaac attcttccta agaagcttct taagaaggtg ttatcatctt
    36661 cttctttact tgcatattga cgcaaccagt ctaaaatcgt tagatcagta tcgaattctt
    36721 tcgttgtatc ctttggtaaa taagaattac ttgttgtcac accccaacgt actgttccag
    36781 tatctggcgt aatttcaccc atgataactt tgaatagcgt tgttgttgtt atatcatttt
    36841 ctgcgacgaa agcgaccttg tcttctttgc tcaaattaaa agatatatca tctaaaattt
    36901 tctttccatc aatcgttaca cttacatttt ctacctgcaa taagtcgttt ccaatctcac
    36961 gttcaggttt gaatccaacg aatggataac gtctagaaga aggttggata tcgtctaaag
    37021 taattttttc aagcattttt ttacgtgaag ttgcttgttt tgattttgat gcattcgcac
    37081 taaagcgggc aataaattct tggagttctt tgatttgttc ttcttttttg gcatttgcgt
    37141 ttgcttgaag tttagccgct agttgactag attccaacca aaaatcataa tttcctacat
    37201 aaagtttgat tttaccaaaa tctagatccg ccatatgtgt acatactttg ttcaaaaagt
    37261 gacggtcatg ggatacaacg atcacagtat tttcaaaatt gatcaaaaat tcttccagcc
    37321 aattgatcga ttggatatcc agaccgtttg ttggttcgtc tagcaaaaga acatcaggtt
    37381 taccaaatag tgtttgtgct aataacactt ttaccttttg tccaccagtt aattcactca
    37441 tttgctgatg gtgcaattct tcagggatgt tcagaccttg cagtaaaaca gctgcttctg
    37501 gttcagcttc ccatccatta agctcagcaa attctccttc taattcagct gctcgaatac
    37561 catcttcgtc agtgaaatcc tctttcatgt aaatcgcatc tttttctttc agcacttcat
    37621 acagacgctt gtgtcccatg atgactgttt caagtacagt atagtcttca tagtcgtaat
    37681 ggttctgttt caatacggcc atccgttcat ctggtcccat agtaacggaa cctgtggaag
    37741 gctgcagatc ccctgataac actttaagga acgttgattt acctgcacca tttgcaccaa
    37801 tcaagccata gcagtttccg ggggtaaatt taatattcac atcatcaaat agtttgcggt
    37861 ctgaaaagtg caaactcaca tcatttacag taatcaattt ttttcctcac ttttatcatt
    37921 attagttttg acaagtctta tagaattata taggggaaag gtaaaagatt caaggcaatc
    37981 gaacattctt tgtgtttcac gtgaaacaga atgaaaaaaa taaaaataaa agcggagcga
    38041 aaatagctac tataggattt attagtttgc aacagcataa tttttgacgt tatttataag
    38101 agggagtact ataaaactgt aaaagaaatg aaagaaggat tgaaaatgga tgaaaaaaga
    38161 accatcgact ggggctcatt gattttggga attttatttg tacttgtctc attgttatca
    38221 tttcaagacc ctgtcggaaa tttagttgca attgtagtag tctttgcgat ttttgccttt
    38281 attaaaggga tatttgaact gtttgtacgg aacagattaa aagagctaac aggatataaa
    38341 ggaaagatgc ccttaataat cgggatcatc gatattctag ttggcgtctt tttcttattc
    38401 aatattggtg caggagtcgc agcattgcct tttgtttttg cagtatggtt tattgctgat
    38461 tcagttttag cgttatttac tgctgacttg gcaagaggag taagtgaagg atattattgg
    38521 tttacgatca tcgtcaatat tttaggaatt ttattaggaa ttttcttatt gtttaatcca
    38581 atttcttctg cgttgacatt aagcttttta gttggattct attttatgtt atttggtatc
    38641 acacatatcg tctatgcatt tagataagta aaatcaaaca aataaaaaag ccggcttccg
    38701 attacagttt tactgttgta attggaagcc ggctttttta tcatggacaa atatgcacga
    38761 ggtgattttt aggtgtcgag acaaatttat gcaaaaatgt gagaagctat atacacaaat
    38821 tttatcttaa tactcgaagc atagcgcagt tgtcacaacc tcttttcata taattcattc
    38881 aatactggca aatcatggac ttcatatttt tctttgaatg cttggatttc ttcttcagaa
    38941 tattttcttg gatctaaatg cagatcttct actggcaatg ggcgcttgtt attgatcttc
    39001 acatcgatta ctacaggacg atctgatttt gcagctgcat caaaggcaaa tcgaagttgc
    39061 gagtattctg tgactgtaaa gctatttgcc ccgagcgctt ctccaacttt accgaaatct
    39121 gcatcttcta aaaatacacc aaatttcttt tgttccgtat cttcttgttc tgcttcgatg
    39181 aaaccaaaag aatcattcga aaagacaaca ttaataattg gaagctgata tttgacttgt
    39241 gtaatgatat cttgcatgac catagcaaac ccgccatcac cacttaaggt aaatacttgt
    39301 ttttctgggt agcttagttg tgcggcaatt cctcctggca caccattacc catagttgcg
    39361 aaccatccag atgtcgtatg tttttgttta ccgttcatat tcaataaacg aatagaatgg
    39421 atagtcgtgt taccgacatc tgtgacgaaa atcgcatcgt cttcagcaat acgattgatt
    39481 tctttataga cagcctctgg acgaatcggt tcttcctctc gatcttcaaa agatttcaac
    39541 caatttaccc atttttcttt attttcttga tttgctaggt accagctatc agcagggcga
    39601 gcatccccta actcgacaag tctttgcaaa gctgtgtttg catcaccgag gatcgacaaa
    39661 gatgtttcat ggcgtcgtcc aaattttgac gcatcgatat cgatttggat aaattcagca
    39721 gttggattaa aaaatgcacg tccaaatggg aagtcgctac cgacaaataa gatcagatcg
    39781 gcttctgcca atgcttcatt tccgggttta gtagcgacac gtccagcaaa accaagaaag
    39841 ttttcataat catcaggaat aattcctttt gctagaacgg aagcgacaac aggaactgaa
    39901 aaatgttctg agaattcttt gatttgcgga aatccgtttc gcgttccttg accaacataa
    39961 agaactggtt tttttgcttt ttcaagataa ggcaaagcag ctagcaaatc tttttcttct
    40021 ggtaatatca cacttgtttt gtgagtgtga gcggtggaga aaggttgttc gtcaatttct
    40081 tcaaaaccaa gatcaacagg gatagtcacg atagccacgc ctttatgctc ataggcggct
    40141 ttaatggctt catctactac atgcggaaga ctttgtggtg tcatgactgt acgattgtat
    40201 acgctaacat ctgcgaaaat aggattttca tttagttcct ggaaagagtt atagttcatg
    40261 gatgtagaag caacttgtcc taagaacgct actagaggca catggtccat ctgtgcatca
    40321 tacaaaccat tgatcaaatg tgtcgcacca ggtcctgcag agccgaagac agcgccgact
    40381 ttccccgtta attttgcatc tgcagcggct gcgagtgcgc cgacttcttc atgacgaact
    40441 tgaatgtagt tgatttcggt tttttcttga tatagcgcat ccataattga attgaaagat
    40501 cctccgggaa tgccgtaaat atggtcgata ccccagcttt caagaacttt taccattgcg
    40561 attcctgcat tgatttttga catggtgttt cactccttta gcaatgatga tacataaatt
    40621 ttttgtatct agagtataga actttttatc gaaaatccca actaattcgt cttttcacaa
    40681 aaatgtagtc atgtttttgg gaatatgcta gagtagtaca tagatagatt caagagaggg
    40741 gaatggcatg gttgaatggc tattgagact tgaaccttgg cagcaagcac ttgctgggac
    40801 agcttttact tattttatga cagctcttgg tgccgggttg gtttttttct ttaaagaaat
    40861 caaaaaagaa gtgctaaact tgatgttagg atttgcttct ggtgtcatga ttgctgcaag
    40921 tttttggtct ttgttagatc cggctattac aaaagcagaa gaaaatggag atattgcttg
    40981 gctagttgta agtattgggt ttggtttagg cggtgtgttt ttatatatgg cagataaaac
    41041 actgccccat atgcattttg gaccacaaca tgaagcagaa ggattgccta cccatttaaa
    41101 acggacgatc ttgcttgttt tttctattac attgcataac attcctgaag gtttagccgt
    41161 gggggtagca ttcggtgcag cagcaactgc ggataatccg acagccgcta tattagccgc
    41221 tgtttcggtg gcgctaggta ttggtatcca aaacttccca gaaggagcag ctgtatcaat
    41281 cccgttgcga caagaaggtc tgagtcgtaa aaaggccttt gtttacggac aagcttctgg
    41341 aatcgttgaa ccaattgctg gagtgattgg agcgctacta gtaacgaaag tggagctgct
    41401 gcttccttat gcacttgctt ttgccgcagg agcaatgatt tatgtcgtgg tggaagaatt
    41461 gatccctgaa gcacagcaga cattgtccag caaacgtcat tttgcagttt ttggcgtcat
    41521 gagtggattt atcattatga tgattttgga tgtggcatta ggataaataa aaaaaatata
    41581 tatgatatat caaatagggc cgcgaactta tatcgcgacc ctattttttt agtaagttat
    41641 atcataatga agtgaatggt atcatacatt aataatagtt ttttctatgt gtatttcctt
    41701 tgatccagcg aacaagccag atgataagtg cgattgggat tagaactttg accgcaaacc
    41761 ataaaagccc gcttaggaca ctgacaaaaa gactgatgac gaagctagct gcaattagca
    41821 ctaaaagaac catgaagata tttaagaaca tatttttttg acgcatacaa aaccctcctc
    41881 ttgttcattc tttactatag aaaaagtata acagggattg tttaaaaaca cctccaactg
    41941 aagactgatt tttacttcat ctacttaaat gatcaaacaa ttgctttttc tctggtttat
    42001 catctaaaat agagcggaaa aggaggagaa tatcatgcct aaaaatcgtc ttgaagcatt
    42061 tacagatgct gtaatcgcga ttatcatgac tattttagtt ttagaacttc atgaacctaa
    42121 cggagacacg tgggaggcgc tcgctgcatt aggttaccga ttttttatct atattgtcag
    42181 ttttatcatt ttagcgattt attggaacaa tcatcatcat ttatttcaag ttgtacataa
    42241 gataaatgga cgggtcttat gggcgaacag tttctttgtt tttgcgcttt cgctttttcc
    42301 gtttgcaact tcatggatca gtgagtacat caatagcctg gctccagaaa tgacttatgg
    42361 ttttgttacc ttgaccgcta atattgcgta ttatatcttg gcaaaagaac ttgtcaaagc
    42421 aaataaaggc cacgagaaag cattgaaggc gatgttgaat aattacaaga aatcgtatct
    42481 atcgattggt ttgaatgtgt taggaattgt cttagggtat ttagtagcgc cagtagccgt
    42541 gctgatcgca aacatcgtga ttctagctgc ttggatcatc ccaagtcgta ccatcgaatc
    42601 acaatatcgg taaaatgatc cgtgtttaaa atttaagaga aaaatataga aaaaataaat
    42661 aaacgatcaa atatccgagt gatactagta ttcaaagagg gatgcagatg gatcaaaaaa
    42721 gaattcatgt aggactgatt actagttttg tcacgatgct tatttatttg gatcaaggtc
    42781 atttgacttg gacagtagtt ggtgtcgtag gatttttgac attattcaca gcaataaaaa
    42841 aataaacatc tttatgtaat ctgatgttct atctatttaa gaaaagcatg tttacagcag
    42901 tatgattctg ttgtaaacgt gtttttattt gtggaaaatt taatttttag aaaagtgtat
    42961 cgaaaaatag atagttagtt tttccacagt ggataacttc gtaagagata aaaataaaaa
    43021 attagtcaaa gaagggcgta tagatttctt ttgataggag atatacaaca gaaatgaata
    43081 aatattaacg tgttttcaaa cagtataaaa cggtgtggtt tttggaataa aaaaataaca
    43141 tatctcattt tacacttttg ttattttata cttaaaaaat aacgacacag aaaataccgt
    43201 gtctcagatt tctctactac ttctaaaaac tactgttttt ttcctttgaa atgccttcaa
    43261 aaagtaggat caggcagaat atactggcag aatcaaggag aagggagcct aaataaggag
    43321 aaaaaccata gaaaatataa agtaattact tataaaacaa gctttttata gaaaaataat
    43381 atacctgagt agggtataaa ttaagacgga attcctttct agattgagat attgactagg
    43441 aagaatgtta agattttaag cctttaagct aaaaaaaaac cctttttgtt aaataagggg
    43501 aatatagcct gtcagtttca aactgcaagt cgtcctgctg caataatata gtagattaga
    43561 gatgaaaata gattttttta aaccaatgac tgaataaaaa gaccgtcata gatacacatt
    43621 ggaatgtgtc tattacggtc tttttttacg aacgatttta ttgaagacgg acagttcggg
    43681 tcaaaaataa aaaagctacg atcagaaata agaataaaat gtatcccaaa ataatggcta
    43741 gacggtctaa cttatttgtc gatagatgtt tgtttagaag ttgataggcc cataaaccta
    43801 tcagtcctcc tgttgtatta gttatcaaat cagtgatgtc cgctacgcca ataccgaata
    43861 ggaattgaat caattctgcc aagaagctta caaggaaaat aatccctaat tttttagaaa
    43921 aagaaagtct cttgaaattg acattcaaaa gcaccccaaa aggaaaaaag acaatcaagt
    43981 tataagtggt ctcacgtaat accgtcgtat tttcaaaaga aaaagtagaa aaagggacga
    44041 gattgacatt gctgtaagaa taagtgagaa attgtggaag attaaatgat agcttgaata
    44101 aaatcaacca taatagaaag aataaataaa tccctaataa aaattttgat acataccgca
    44161 tagacatctc ctccatttaa aaagtatacc atcgaaaacc gctgaaaaat cataaaaaag
    44221 tatgaagttt aaggaccaac gtagttatcg tacgaaaatt agaagctttt tttgttggga
    44281 atccggtttc tttaacaaag ctaagatagc gagttgcttg gaaaagttgc tatctttttt
    44341 ctgtcaaaac gactcggaat gttcgttttt tgttctaatt tctccaagtt gtttactttt
    44401 tgatactaag tttatgaaaa atcaagaaat agttgttata ctaggtaatg ctgacaaacg
    44461 ggattggcat ttcatcttgg ttcagctttt tttccaatta ctaggaagga gggatagatt
    44521 gttcaagact tattttcgtt tcaataaaat acaagataat ccttttcgta tcatgaatct
    44581 gatgctactt atctttattg tattatggtt aggttacata aatcatgatt ttttgatttc
    44641 aagtttttca tcattaggga tctttacttt tttctattat caaaacatac cgatgaaata
    44701 tttgctgaaa cgtatgtttc tgatcggttg cggactgttg gttgctttcg ttttggggat
    44761 cttatcgact tatgttttat ggcttgaacc atttgccgta gctttggttg ctttttccag
    44821 cagattcgtg ttgcgattat tccatatttc taaaccaggt ggcttgtttt ttgcgatgct
    44881 ttctgccatg ggaacgagca tgcagctgcc aatcgctcaa ttaccgatcg tctcacttta
    44941 tttttttatg ggtgtcgttt ttgccctgat tgcggcagtg attacaaaat tactggattc
    45001 acggcctgag cagacaattg aaaaagcaac tttaaaagaa agatttcacg aagagccttt
    45061 agtgatcatt gattctgttt tctacagcgc tgccttattt ctttccgttt atgtcagtca
    45121 cggcttgaat ctgcataatc cttattggtt gaccttgtca tgtgcatcga ttttgctagc
    45181 agaaaacttg gatgcgatga agcatcgaca agtccaatat ctgattggat cgatgggtgg
    45241 gttatgtgtg tcggcattct tgtcatttgt gccgtttacg cagttgcaga ccatttttct
    45301 gatcactttt ctatatggaa tcgcacaatt tctagttgct agaaattatg cagttgctaa
    45361 tatttttctt aatccgatgg cgttgatgct atctacactt attcgtggag catacttgat
    45421 ttctttgatc gaatatcgtt ttttagggat cgtcatagga agtttcatcg gtttaggtgt
    45481 tgcgtgggta atgacggttg gtttgcaaca ttatttaacg gttgttcgtc aaaaattaga
    45541 atagaaggac aaatatatgt gccaaaagag gttatgacaa gatttttgtc acaacctcta
    45601 attcaaggtg ttcaaaagcc agattctcag cattaagtca aattcacaca aaaatgtgga
    45661 aaggaacttt caaaaaaatt ttatatttat tctatcaaaa aattttgttt atgatggtaa
    45721 ctgaataaac tttcctgaga tggtcaatgc tttccttgaa agtcttacag atattctttc
    45781 ttcatagaaa gtttaagttt tatagttagt gtacaataaa agtatgttta taccatatgg
    45841 tgtccggata atcagtcatt taaacaatga ggttcaaaga tatgaaaagg ggaaatttga
    45901 tgaagaaaat aagcaatgaa atccaaattc tggcagaaac tttaactctt cccaaagtcg
    45961 ttatagagca aatgattgta caagcaaaaa acatccttga tgaggtgttt gagcaatata
    46021 gtcatgaact aatggatcct aataaagcaa aggcgactgc aaagaaaata gacaaagtct
    46081 atgaagagga accttttgca gcattagcta tttatctata tgcagcttct caaagttgga
    46141 aaaaaattta tcaaccattg aagattccaa gaactatcta tttggcaaca atgaatgctt
    46201 ttactagatt tattcatgaa catttccaag taacaggaag ctatcgcttt gaccgtggtt
    46261 tttggatttg gcattacact agtggcttga tctttcggat tggtgaattg gagtacgaaa
    46321 tgacatattt tccgaaaggg tacacggatc taagattaga aggaaagcag tgcctttctc
    46381 ttcatattcc ttccgatgca gatttatctc agaataagat aagagatagt tacgaaagtg
    46441 ccattcgttt ttttgaactt tattttcctg attaccatta ccaagtgatg tacacagata
    46501 cttggttact atcacccaat cttactaaat ggttgaaaaa agagtcaaaa atctgtcttt
    46561 ttgcagccga ttatcgatta ttatcagttg atgaacagga cgatagtgga gtcccttgga
    46621 tctttggacg agtagatgcc caaatacatg attatccaga aagtacgagt ttacagcgac
    46681 aagcaaaaga gcaattgctt gcaggcgagc acattggctc tggcttaggt attcttactt
    46741 cataagttta gtacttcgaa atagtttaaa agagcgtgag acaaaagtaa agaacacttt
    46801 tgtctcacgc tctaaacacg tataaacggc ggaagcagaa gcaactcctt cggaaataag
    46861 ctgaaattca caaaaatttg aaaagcaatt ttcgtgaatt tcttcttatt tcttggagtt
    46921 aaacgcttct gtcccagcct cttttttagt gttctatcga acacacagta cgttcaatag
    46981 gtaaactagc tataacaata gatattaaag ttaacggaca agagggcctt ttttaggtag
    47041 ctaaaacgta ataatgaaaa tttatttcca gagataggag tcaatcagct agtctgatcg
    47101 actgttttaa caagttgact ttcattttaa cttttgatac catttaaaac acaaatcgta
    47161 attgtttcga tttgtgtttt ttagcatgac tacaatcaaa gaagaaagtg ggtcgtccat
    47221 atggaaaaaa cagatatctc aagtgcatac cgccgcttga aaagcccaaa tatcaaaaca
    47281 cgaaaacgtg cgctgaagat catcaaagat gtgaaacgga atagtgggaa aaggtaagaa
    47341 gaaggaaact taattaaaaa aatagccgaa aaacgttttt cggctatttt caataatcgg
    47401 tttttgtgta aggtttccga aagaactgct gttgattttc catttttaac atatgataaa
    47461 ttggtagaat ctttcttttc aaattgatac cattccctaa ttccagttat atagttaact
    47521 acagaaccac aaagcacgta cgtttataaa agtattatct gtagttattt ttcaattccc
    47581 tttcaacctc ttattttgac cagtagtatt gatcacttta ccttttaaca cctttgttcg
    47641 tttttgaaac atttttagtc aagaatggaa acgtaactac ataatcaaag gttttgcgaa
    47701 atatttcatg aaatatttca tgtagaagcg agcaatacgg ttgcaaataa ccattatcgt
    47761 ttaataagca gttacgaaag ctcccaatat ctctaacgtt cagctcactc catttctttt
    47821 cttcaaaaaa atcagtgatt cgtatctctt tctatttgtc gaaaaagtta actttaaaaa
    47881 gtaacgccga aaaaatatgc aaaaaaataa aaaagcagtt actattgcta gaataacatc
    47941 aaggaataat aagttttggt tttcagataa tagtcgataa gttgtgtatt ggaattttag
    48001 atacagtaat aaagtatttt tcatgaaatt ttgtgagaat cactattaca agtatcttta
    48061 ataaaattaa tggttaagga ggaacttaaa gtagaatgta aatgaatagt tgtagtcagt
    48121 cgattgtgat actagactca aagttgcagg aataagtttt tcaatttgtg attggaagat
    48181 aaaaaatgaa aaagaagggc tttttttata ctttgttgat tgtagtgttt attttccctc
    48241 tgtttagcca agtaacattt tctatgactc aaccagtttc aagtgaggaa catgataacc
    48301 tttttcgtgc tacagaagag tttaagaaat atcaggaaaa tattgttccg gagactccat
    48361 tagctagtga gccaattact gatattgatg gaaaccaaat aggatggtat atacagtacg
    48421 aagttatggt ggtaaataat gactcaatag taaaaaatac agtaggctta gagctaacac
    48481 cgattgaaaa ggtgagttca ttgttaacgt atatttatac tgatgaaagt gacgagactg
    48541 agatcatttt aacggattac tcaaaaattg ctactgataa aaagtatata ttgtggatgt
    48601 agacactagt gaaatacttt atgatataga aattgatgaa aatttactag atgtagcaca
    48661 agaaatcgat catcaaaagc aggaagctat tcgatatgct ggtagaaaag ggaaaagaaa
    48721 atatttatta cgtgaatgta gcaaaagtta gagaagatga aaatgagtgg aaagaattca
    48781 agagtcgcta ctcaattaac tcgactccga catttacagt ttacagagaa ggatcgatag
    48841 aaaaaactgt tttttggact aaagaaagtg gaatgtcttt agctgaagta gaagagtttt
    48901 tggattatgt tagtatgcaa cagtaaggga gttcttatta tgaaaaattt tgacacatat
    48961 tatgttattg ttttttcgat atctttgctg ttaatattac taactttaat gcagtataaa
    49021 gagtatccag agtattttat tttaactttt gtatatttag taagtagcta cacacttcta
    49081 tataaattca gaagttcttc tagagtttct aaagtttgtt tcttaattag cttattgatt
    49141 ctataatatt tttgatttat ataggaaatt aaaaatatta tagaatcaac actcagagaa
    49201 cgggttggtt tttaatagat ttaataagta ctgataatga aatttctcct ggttgggaaa
    49261 acgaagttgg ttaccctgta gaagaatttt ttcaagatta ctttgcggag tatgccgata
    49321 ggataatgag agattacgta acagataaaa ctcaagaagt catcaatcaa tcccagtcat
    49381 taaatgaatt atctgataag gcgaaagaag aagcagttgc cattatttct tcagggatta
    49441 gtgactatat tgatatgagt ggtgaaatca attatggcga taaagacaaa attgtacgca
    49501 tgataaaagc ttttatcaaa acttttgatg tgaaggcaga tcttaagttt aatgatactt
    49561 acctagtagg aaaattaatt aatatccttg ggacagagaa agatacggaa acgtttattt
    49621 cctctttgtc tatgttgttt acagataagc atttttcaac tcagcttggt ggagaggaac
    49681 tagtggaaat cttttaacca ttacgcccag cattaataaa agtatatcaa aaaagtactc
    49741 ctgatgattt ttaagattgg tttgagaaat aatgtgaaaa cactattttt aatgttaatt
    49801 atagatagag atgaacgcgt catatggtta aaagaatgaa aaagtcaaag atacactgct
    49861 atatttcact aaggatatag cagtgtatct ttcatgtcgt ttcactggca gttttttata
    49921 aaaggaggtg tgcatttcag ttgcttttct ttggtagtta gttgaatcgt ttaagaatta
    49981 acggatgtgg cagacaggtg ctctgtccaa aaaccagtaa aatctttgat ggttccatct
    50041 ttatttagct gtgcctgttc aaatactgtt ttgtttttac cgaatggatc aattgcaata
    50101 attgttccga tccccgcacg tttggcagca agtaatcctg agtaagcatc ttcgataaca
    50161 agacaatcag ccggagctaa gctcagtttg tctgccgctt taagaaaaat atccggttgc
    50221 ggtttcccag ggaaagagcc gtcgtcatag acaatcttgt cccagtcaaa ccagcgggct
    50281 aaatcgaaaa tatcaaaata aaaagcaaca ttctctttga cagtagccgt ggcaatcgtt
    50341 aatggaatct gttggtcagt cagctggtca agagtcgctt caagcccttt tgtcaactga
    50401 agctcgtttt cattttgcaa gcaaagcata cgataatagc tttctttttc ttctgacaat
    50461 acggcgatct cagaggcggt caaatgctct gaaatgaaat gtttcaaaat ctccgtattc
    50521 gtccgaccat gaatattttt taaaatatcc tcgtctgata aaccatcttt tgtatgatca
    50581 tgtatcatat gcagccaagc agcttcatgc agatgagagt ccaaaaacat tgttccgttg
    50641 aaatcaaata taatcccttt catagttacg ctcctatagt ggtattttcg gttgaatcag
    50701 caatttgcag atcgacatat tgtgcatagt gctcttgaac ctcccgcgag atctggcgat
    50761 ccgtaatgag cagatcgaaa tcatgcaaat tataaaacac ataaaaatca tctcgattga
    50821 attttttatt atcaacgagt agatatttct tacgggaatt atttagagcg atctgctgtg
    50881 cttctccttc ttcggtactg taagtagaga tcgcttcgtt gtgaacgcca tttgaactga
    50941 taaatgcttt tgtaaacttt agtcttctta aattgtcgtt ggtgatcggt ccgacaaatg
    51001 cgccggtatt tttccggaaa tcaccgccaa tcagtagaat ttctggtcca tctgcttgag
    51061 caagaatatc aaaaactgga taattatttg taatgatccg aattttcttg ttcagtaaat
    51121 gcgtcgccaa tagttcgatt gttgtacctg gtcctaagaa aatcgtttct ccctcgtgaa
    51181 tcaaggaggc tgctgtggca gcaatttctt ttttttcttg aatctgcaga gtggcttttt
    51241 cagtgtgaga aagttcttga tctaaagtgt aggaaagact ttgagcccct ccatggacac
    51301 gcagcagctt gcccgatttt tctaattcat ctaaatctct gcgaacggtc atatcagaaa
    51361 catctaattg attgatgatt tcattaacgg taaggattcc ttttttattg accatttcta
    51421 cgatagtaag caatctttct tttttcaaca ttgtcttcac ctctaattta aggataatct
    51481 atcatataaa cggacaacag tcaatctaat tcgaacaaaa gtaaacattt agggtggttt
    51541 ttatgtttgt tacaatagat tttattgctg ttttctggtt tttatgatta tttgtgtttg
    51601 tttttgttga caaatgtttc ggatgcgttt acaatgcaat cagttagaaa gagcgaaaac
    51661 tgattataga tttgaggaga gatgtacatg cttaaattac cagaagattt tatttttggc
    51721 ggagccacag cagcctatca ggttgaagga gccactaagg aaggtggcaa aggcgctgtt
    51781 gcttgggatg actttttaga ggaacaaggg cgattcagtc ccgatccggc aagtgatttt
    51841 tatcatcaat atgcaaagga cattgaattg tgtgaacgtt ttggtgtaaa tggtctaaga
    51901 ttatccattg cttggagccg catttttcct gatggagctg ggaaaccaaa tccagaagga
    51961 atcgcttttt atcatcgtgt gttcgaagaa tgtaagaaaa gaaacgtaac gccgtttgtg
    52021 acgttgcatc attttgatac ccctaaacgt ttgtttgacc atggagattt tttgaatcga
    52081 gaaacaatag aagcatttgt ttcctatgcc attttttgtt ttcatgaatt caaagaagtc
    52141 aaggtgtgga gtacctttaa tgaaatctat ccagttgcaa ccaatcaata tttgttaggt
    52201 gttttcccac caggaatcaa atatgatttt acaaagatcg tcgcctgtct tcataatatg
    52261 atggttgctc atgcccgtgt agtgaactat ttcaaagaaa acgaacttcc gggtgaaatt
    52321 ggtgtagttc actcattaga gacgaaatat gcggcaacag acgcgccgga agataagcat
    52381 gctgcctttt tagacgatgc attgtcgatc cgtttcttat tagatgctac ctatttaggc
    52441 tattattcaa ctgaaacctt gactgctctt gatgagatat gtgaagcaaa tcaggccagc
    52501 tatcattttc ctgaagaaga tttcgttgaa ttgaaaaaag catccacgcg taatgactac
    52561 ttagggatca atcactacca atgccatttt gtcaaagctt acgatggaga aaatgcaatc
    52621 catcataacg gtacgggtga aaaagggacc tctgtttata aagtgaaggg aatcggagaa
    52681 cgtatttata aagaaggaat tcctcgaact gattgggact ggctgattta tccagaaggt
    52741 ctatatgact tgttgctgcg aattaaaagc gattacccac attacaacaa aatctatatc
    52801 actgagaacg ggatggggta taaagatcag tttgaagatg gcattattat ggatcagccg
    52861 agaatcgact atttgagagt ctatttggaa tccttaagca aggcgataac agccggggta
    52921 aatgtgaagg ggtatttttt gtggtcgctt atggatcttt tctcatggac aaatgggtat
    52981 aacaaaaggt atggcttgtt ttatgttgat tttgaaaccc agaagcgtta cccaaaagaa
    53041 tcagcttact ggtacaagtt ggtcagtgaa accaaaacga ttatttaata agtgataatg
    53101 gaggaagaaa agatgaataa agaggaatta caaatgcttg gttttgagat cgttgcatat
    53161 tcaggcgacg ctcgcagtac attattgaaa ttattgaaag aagtaagaca aggaaacttt
    53221 gaacatgttg attcggcgtt gaaagaagcc gacgaaaatt taacccttgc acataattcg
    53281 caaacaaaaa ttttagcgga agaagcatct gggaaagaga tggagatggg attcattttt
    53341 atccatgggc aggatcattt aatgaccact ttattgctga gagatctgat tcaagacttt
    53401 attgtactgt acagacagaa tcaaggatag gagaagaacg ctatggatgg aatcattaaa
    53461 caaatcgaaa aagggaaacc attttttgaa aaaatctcac gtaatagtta tttaggagcc
    53521 gttcgtgacg gatttttaac agcgatgcca gctattttat tttcaagcat tttcattttg
    53581 attgctgcgg ttcctgagat ttttggttac caatggcctg aatctgtctc cacatggcta
    53641 tggaaagtct ataattattc gatgggtgtt gtagggttac tcgtttcagc aaccactgct
    53701 cgctgcttgg ctgattcgat gaatcgtcgc ttaccgaaaa acaaagtaat caacaccacc
    53761 tctgtgatgc tggcctctgt ggtaagtttc ctgttgttaa gcgtctctcc gattgaaggt
    53821 ggattttcaa cggaatatat ggggacaaag ggactactgg cttcctttgt cgctgcattt
    53881 ttaacagtta atttatataa gttttgtgtg ctgaaagata ttaccattaa aatgcctaaa
    53941 gaagttcctg gaacgatttc gcaaaccttc cgagatattt ttcctttctc tttttcagta
    54001 tttgcggccg ttttaattga tttaggcatt cgacaaatct tccaaatctc atttgcagaa
    54061 gcaatcatta cggtgctgca gccgctattt acagcagctg acgggtactt aggaatcgct
    54121 attatctggg gagcgatggc gctgttttgg tttgtcggtg ttcatggacc gtcgatcgtg
    54181 gagccggcaa ttgcggcaat tatctatgcc aatgtggaaa ccaatctgca attgttcaat
    54241 tccggtgaac aagcttctaa agtactgacg gtgggtttag ggaattttgt aggaactatg
    54301 ggaggaactg gggcaacatt ggttgttcct tttatcttcc tactctttgc taagtctaag
    54361 cagttgaagg cagttgggaa agcttccttt attccagtaa gtttcgctgt caacgaacca
    54421 ttgctatttg cggcacccat gattttgaac ccttattttt ttattccatt tttgttaaca
    54481 cctgttgtaa acgtttgcct gttcaagttt tttgtcgatg tattgggcat gaacagcttt
    54541 atgtacgttt tgccttgggc aactccagca ccgatcggtt tggttctagg aacgggcatg
    54601 ggtatcttag cttttgttct agcagcggtt ctaatcgtcg ttgacttcct gatctatcta
    54661 ccattttgca aagcatacga tgctgtctta gtagagaaag aagcacaaaa agcagaggtg
    54721 atcgcaaccg aggaaggtgc ttctgccgat gtacgtgagg aagtacctgt ggcagcgagt
    54781 caagttatca gcgatgcaaa taaagaaatc aatgtgttgg tactttgtgc tggcgcaggg
    54841 acaagtgcga tgttagctaa tgctttaaaa aaaggtgcat ctgaatcgaa agcaccgatc
    54901 agtgcaatgg cgggtgctta tggttctcat tacgaaatgc tgcaggattt tgatatggtg
    54961 gtcttagcac cgcaagtcaa ttcttactat gatgatatca aagaagacac cgatcgctta
    55021 ggaatcaagc tggttgcgac aaaaggagca gaatatatcg gattaacgag agatcctgaa
    55081 aaagctgttc agtttattct gaaccaattt caataaagga ggagccataa tgaaaatggg
    55141 aatttggttg cgtaaagctg ttttatttgc agctgtgtta ggggctttgg gggctgtttc
    55201 aaaaactgtt gaagcatctg ccagcgaatt tattcgcgga gcagatattt cgatcctgtc
    55261 agatatggaa aaatctggtg caaaatatta tgaagacgat atttcaaaag atgccttaga
    55321 gattttaaaa aataatggtg tcaactatgt tcgactgcgt ttgtggcagg atccttataa
    55381 tagtaatgga gaaagttatg gagcagggac aaatgatctg gaaacgacga ttgaccttgc
    55441 aaaacgagca aaaaatctgg gacttaaggt gctgctggat tttcactaca gtgatttttg
    55501 ggttgatcct gggaaacaga atcttcctaa agcatggcaa ggtctgacct ttgaagaaat
    55561 gaatacggca ttgtacgact atacaaagaa cgttctgtcg gaaatgaagc agctggacgt
    55621 ttatcctgat atggttcaga tcggcaatga actgaatagc ggaatgctct ggccttatgg
    55681 caagtcttgg ggagaaggcg ggggagaatt tgatcgattg gctgcttttt tgaagtctgg
    55741 tatccaagct gttcgagaca ctcaacctaa aacaactcca gtaatgctcc acttagctga
    55801 tggtggcgat acaggagcat ttacttggtg gtttgatgaa atcacgagtc gaggtgtgtc
    55861 atttgattta attggagtgt cgtattatcc gtattggcac ggttcaatga atgaacttca
    55921 aaccaatatg aatgcgatca gccaacggta tggtaaagaa gtgatcgtag ttgaaacagc
    55981 ttatgggcat acaacagcga atgccgatac tatgccaaat gcttttggtg aagccgaagc
    56041 agctgcgggg ggatatgaac caacaccgac aggacaagca gagtatttgc tggatcttgc
    56101 cgatcggatt caggcagtac cgaataatcg cggggcgggc ttcttctatt gggaacctct
    56161 ttggtacaac ggaaatgtga gttgggcaac acaagcaggt atgaattatc tcagtgttca
    56221 atccactatg ggaaacgaat gggacaacca agcgatgttt gattttgctg gaaacgcctt
    56281 gccaagcttg cgtgcgttta aacaagcagg ggcgcaaata aatctagtta aaaatgcaag
    56341 tttcgaaagc gatggaccaa cgaccagtcc ttccaattgg caaacctggt ttcaacaagg
    56401 aaccgctaag ggtacgatca aaacggaagc gggcactttt tttggggact acaaactaac
    56461 tttttggcag gatcaagcat acgaggcatc agcttatcaa aatgttagtg gcataagcaa
    56521 aggaacttac acattgagtg cctatgtaat gtctggggga gagtttgata ctaatcagtt
    56581 atatatcaaa aattatggtg gagcggaact aaatactgcg gttgttaaaa caagtgaatg
    56641 gacaaaaatt acaatcaaag acattccaat tacaacaggt gtttgtgaaa ttggggttta
    56701 tagcaaggct tcaggaaatg cttggacgaa cgttgatttt gtttcattgg tgaaacaata
    56761 gactgcggat cgtttcgatt caagatcgga ggttcccttc agccacatcg ttagcgtaga
    56821 aggcaggaat cgaacctgca cctcgccttt gccaaaaata accagaaacg atttccagtc
    56881 atttttacca aaacgagtaa agcaccagac ttcatctaca ctttgtatta tgctaaagag
    56941 agttcaacga tttcgtaaga agcaatgaaa aaattgcaaa aagttgtgca gataacataa
    57001 agtttgttta agaaagggta accttagaat tccgtcgtga atatgatttg agcacttaga
    57061 tggatagaaa aaagttctcg gaaaacagtt cgtttttcga gaacttcttt ttatgataag
    57121 aatgaaacaa attaacttat attatagtct cttttggtag ggaaccatct tatttttaat
    57181 gaaaatcaag cattttagct attgaagaat gttgatttat agggattccc cctccatatt
    57241 atgttggctc tttattccca ctccactgtt gctggtggtt tagaagtgat gtcatacacg
    57301 atacggttga cgtgggctac ttcgtttacg atacgtacag agattttttg caagacttcc
    57361 catgggatac gggcaaagtc agcggtcatt ccgtcgattg aagttacagc acggataccg
    57421 actgtgtagt cgtaagtacg tccgtcaccc ataacaccga cagagcggat ccctggaaga
    57481 acagtgaaat attgccagat atcacggtca agtccagcag ctgcgatttc ttcacgcaag
    57541 atagcatcag attcgcgtac gatttctagc ttgtcttcag tgatttcgcc aagaacacga
    57601 ataccaagac caggaccagg gaatggctga cgccaaacga ttgaatcagg catgcctaac
    57661 tgagtcccta attcacggac ttcgtctttg aataatgtgt ttaatggttc gatcaattca
    57721 aattgcatat cttctggcag tccacctaca ttatggtgag atttgatcgt ctgtgctgtt
    57781 tctgtaccag attcgataac gtcagtatac aacgttcctt gtgctaggaa agagacgcct
    57841 tcttcaccag caagttttgt tgcttcgtca tcaaaaacat atacgaattc attcccaatg
    57901 attttacgtt tttgttctgg gtcagataca cctttcaact tgtctaagaa gcgatctctt
    57961 gcattgactt tgatgatgtt caagccgaat tttccgccta gactttccat tacttgttcc
    58021 gcttcgcctt tgcgtagcag atcgtggtca acaaagatag atgtcaattg gtcaccgatc
    58081 gctttttgta aaagaacccc aacgacacta gaatcaacgc cacctgaaag accaagcaag
    58141 acttttttgt caccaacttg ttcacggatt ttttctactt gcatatcgat aaagttatcc
    58201 attgtccagt taccttcaca gtgacaaaca tcaaaggcaa agtggcgcag caattcgatt
    58261 ccgtattcgg aatgacgtac ttctgcatgg aattggatac cataaaaatt acgtgttgtg
    58321 tcttggatag aagcaatcgg acaatctttg cttgttccga ctgtttcaaa gccttcagga
    58381 actttagtta ccaagtcacc atggctcatc catactgttt gttgttttgg tgtttcttta
    58441 aacaagacag cttgatcgct tgttacttct aacaaggctt tcccatattc gcggttttta
    58501 gcaggctcta cggaaccgcc aagtttgtac gtgatcaatt gcataccata gcaaatccct
    58561 aatacaggaa tacctaaatt gaagatctca ggatcgatcc caaatgcatt ctcatcatat
    58621 acactgtttg gtccgccaga aagaatgatt ccctttggat tcatcgcttt tacttcttct
    58681 gcagtaatgc ggtggctcaa taattcggaa aagactccaa gatcacgaat acgtcttgtg
    58741 atcaattggt tatactgact accgtagtct aatacgataa tcttttcaac gttcgtcata
    58801 tcggcaacgt ttgtcaaatt tattacccct atccctttaa attttatctc aaaatagtca
    58861 aactatttta aaatgctaat attttcgcat gaaaatcgaa tcgatgatat gattttcagt
    58921 tttatggaga attatatcgg ctcttcctcg tgtaggcaag atatattcct gtaaattttt
    58981 caaattcaca tttttccata catcttttgc cattttaaag gcttcttcac gatctccgat
    59041 agcaaaacga taataataat tctctggatt ttggaaagcc gtatccagta acgcaccaaa
    59101 acgatccaaa taccattttt cgatcagcac gggatcggca tcgacataga cagaaaaatc
    59161 aaaaaagtcg ctgacgtaga tttgctgatt cgcaggtaat tgaagcgtat tgatcccctc
    59221 aacgatcaag atatcaggct gctggatcaa ttcgtacttg tctggaatga tatcatacac
    59281 atcatgtgag taaacaggta cttttatctc ttcctgacca gattttacac ggttcaagaa
    59341 atcgatcagc atctccatat cataactttc aggaaatcct ttacggtcca tgattccacg
    59401 ctcttttaat atacgatttg gataaagaaa tccgtcagtc gtgatcagct gaacgtttct
    59461 tcgtttgaag gtacgagaca aaatcgtctg cagcagtctg gctgttgtac tttttccaac
    59521 ggctacactt ccggcgatcc cgataataaa aggcggtact tctacgtatt tatgaagaaa
    59581 taaaccttta ctcaagaaca aagattcaaa ttctttcata taaagataaa tcagatgagc
    59641 aagaggtaca tagatatctc gaacatcctg catggaaatg cggtcattca aacttttgat
    59701 attatctagt tcatcttgtg tcaaaggtgc ttgcccatta cgataaaatt ctcgccattc
    59761 ttctcttgcc acacggtagt agttcattgg atcattcata ttagcctcct gcgttctccc
    59821 atcaatggaa ctattttaac acaaaataaa cgcaaacccc accacttaag agagattgcc
    59881 gatctttttg tataatcttt ataatagaaa gaaacggagt cttattcttg acttgcgcac
    59941 ggatacataa aaggatagac tgttgtcatg aaagaaatga gaaggatgga agacatgaca
    60001 aatcattact attctgaacg cccagatacg gcgcacgatt ttgaacaatg ggcttttgag
    60061 ttaaaaggca gaacctttca atttgtaaca gattctggtg tattttcacg tgaaacagtg
    60121 gattatggtt cacgtgtcct gattgatgct ttcgattggg aagagttacc tgatgaagga
    60181 aagatcttag atgtgggatg cgggtatgga ccgattgggt tagctttggc atttgcgaca
    60241 cagcggtttg ttgaaatggt cgacattaat gaacgagcgg taagtctagc gcaaggaaac
    60301 gcaaagcgta accaaatcga acatgttgat atccatcaat cgaatatcta tgaagcagtt
    60361 catgaagaaa cctatgcagc tatcgtaagc aatccgccga tccgtgctgg aaaaaaagtt
    60421 gtacatgaaa ttttgacagg cgcatatcct cggttgaaga agggcggtac actgacaatt
    60481 gtgatccaga agaaacaagg agcgccgagt gcgcaaaaga aaatggaaga aacatttggt
    60541 aatgcagaaa tcgtcacaaa agataaagga tattatatca tcaaaagtgt gaaagaataa
    60601 ctagctgaaa aatagtggag cgacgaaaga gtggaactgg gcgtgcatgt acgggtacgt
    60661 atcagttctt tttttgttga gaagttttta aaaacaaaga actatttttt cttcaaaaag
    60721 aaatagtagg aacagagtcg ttgtttttta cgataaaata aataagatac agaacttgta
    60781 tagaaggctg ccaagtcggt agataaagga ggtttttatc aaaaaacata ctttcctttt
    60841 ttctctattc ttttcttggg ggttttggta cactgatagg gaatttagat agaagggagc
    60901 caataggatg aagctgacca tcagagatat cgcagaaatg gcaggcgtat cagtaacaac
    60961 tgtctcacag attttgaata acaaaggaag cagatttagt gataagacaa gaaaaaaagt
    61021 gttgtcgatc gtgaatgaac atcattataa gcccgattat ttcgcttcaa atgtgatcaa
    61081 tcgacattca aagaccatcg gaatgatcgt ccctgatgtc actgatttct tcttctccaa
    61141 actgatcgaa ggtgtagaga gctatttgaa tcctttgggc tatgtgatca tgttatgcaa
    61201 ttctcgtcat agccaagaaa atgagatcaa gtatttacaa gagctttccc accgctcagt
    61261 ggacggaatc ttgctggcca caccaaatat tttacctgag aaatatgcat tagacagcgg
    61321 tttttatcaa aaaatgccaa tcattttaat cgatcgcgga ttgaatcgtc gggataacgg
    61381 acggcttatc gtaaaggaat atgagggcgc ctatcaagct gtttcgctgt ttattgaaaa
    61441 tggccatacg aagatcggca tgctgaagga aaccactgga tactatcaat tagaagaacg
    61501 tttcaacgga tatcgtcatg cgctgaaaga tcatggcctg ccttttaatg ggaaatttgt
    61561 tgaacatgga gagttgactg tccaaggtgg atatactgca tcaaaaaaat tactcaaaca
    61621 taaagatatc acggctattt tttgcgggaa tgatgccatg gcgatcggat gctatcaggc
    61681 gatcgatgaa ttgggcaaaa aaatcccaga agatatctca gtcataggat tcgatggatt
    61741 aaagctgtca gaatacatga ttccgcaatt gacgacagtg cagcagccaa gttttgatat
    61801 tggattttat gctgcacgtt tcctgatcga tacgatcgaa tttcctcaaa gaaaagttcc
    61861 gaacaaagtg ttcgaaacaa aattaattat tcgtgagagc gtaaaagtat tgacaaaggg
    61921 ctaactcggc ggtatattgc ttatgtaagc atttacaaag gctagttagt gcttttttta
    61981 tttttagtaa accagtttac taaagtggtt tacctttcct aaaaataatg tataataccg
    62041 aatgaagaca aggaggatca ggcaatgcga atggtggatc tgattgaaaa aaagagggat
    62101 ggaaacgagc tgtcgaaaga ggaaatcgag tacatcgtca cgaactatac gaatggaaag
    62161 attccagatt atcaagtcag tgcattattg atggcaattt tttatcaaga catgacaaat
    62221 gaagaaatca cgaacttaac attggcgatt gctaattctg gtgatgtcat cgatttgagt
    62281 tctcttgaag gaataaaggt agataagcat tcgacaggtg gcgttggtga cacgaccaca
    62341 ttgattttag cccccctagt agctagtgtc ggagtgacag tggctaaaat gtctggacgc
    62401 ggtctaggtt acacaggtgg tacactagac aagctagaag caattcccgg atttcagatc
    62461 gagctttcgg atgaagaatt tgtacgcatt gttaatgaaa gtaaagtagc ggtaattggt
    62521 caatcaggaa acttggcacc ggcggacaaa aaattgtatg ccttgcgtga cgtcacagcg
    62581 accgttgatt ctcttccgct gattgccagt tcgattatga gtaaaaaaat cgcggcagga
    62641 gcagatgcaa tcgttttgga tgtgaccact ggagacggtg ctttcatgaa gaacatcgaa
    62701 gatgcaagac gtctggcaaa gaccatgacg agcattggga aactggctaa ccgagaaaca
    62761 gtggcggtta tctcagatat gtcagaacct cttggcgaag ctatcggaaa tagtctagaa
    62821 gttgtcgagg caatcgaaac attacaagga aatggtccag aagatctagt agaaatgtgc
    62881 tacgccttag gaagccaaat ggttgttctt gctggcaaag ctaagacaac cgatgaagca
    62941 agaacgttgc ttcaagaagc tctggaatca ggtaaagcgt tagcaaaatt caaagaaatg
    63001 atccaaaatc aagggggaga cccagcgatc gtggaacatc cagaacgaat tttgactgca
    63061 cgctacacaa tggagcttcc tgctaaacaa agcggagttg tgtcaaaaat cgtggcaaat
    63121 gaattaggaa ttgctgcaat gatgctaggt gctgggcgta aaaccaaaga agaagatatt
    63181 gatcacgctg ttggattgaa actgcataaa aaaattggtg acactgtgac aaaaggtgag
    63241 tcactattga cgatctatag taacgacgaa gaaatcactt ctgtcattga attattatat
    63301 aaaaatattg aaattggtga atcggcaatg aagccaacgt tgattcatga catcatcaca
    63361 gaataaagga aaaaagcgga ctttaccgtt ttaaagaaaa ggggaaaaga aatggaacta
    63421 aaccgaatga tcgaccatac tattttgaaa gcagacgcat ctaaagaagc tgtgatgcaa
    63481 attatcgaag aagcaaaaaa ataccatttt tattctgtct gcatcaatcc agcatgggtt
    63541 gcgctagcga aagaacaatt acaaggtgag ccagtagccg tatgtacagt aatcgggttt
    63601 cctctaggag caaacacacc cgagacaaaa gcatttgaaa caacagatgc catcaataac
    63661 ggtgcagatg aagtcgatat ggtcatcaat atcggtgctt tgaaatcaaa ggatgatcaa
    63721 caagtccaaa aagatatcga agcagttgtt gaagcagcaa aagatcgtgc gcttgtcaaa
    63781 gttattgttg aaacatcgtt attagataga gaagaaatca tccgagcttg cgaaatagca
    63841 aaagctgcag gcgctgattt tgtcaaaaca tcaactggtt tctcaacagg cggagcaaag
    63901 gtagaggatg tccgtttgat gcgtgaaact gtaggaccag aaatgggtgt caaagcttcc
    63961 ggcggtatcc ataacgaaga agaagcaatg gcaatgatcg aagctggtgc tacaagaatc
    64021 ggaacaagcg caggagtagc aatcgtatct ggttcaactg gtgaaggcta ttaatttttt
    64081 caaaggagaa gctcatggca gaactcaaac aagaatggat caacacagct attgaagcgt
    64141 tagataaagc ctatgttcct tattcccatt ttccggtagg cgcttgtctc gtaacggaat
    64201 caggcaagat ttatcaaggc atcaacattg agaatgcatc atttggatta acgaattgtg
    64261 ccgagcgcac agcttttttc aaagcagtat ctgaaggaga aaaaacattt acgcatctag
    64321 tcgtagcggg gcacaccccc gatcctatct ctccttgtgg tgcatgtcga caagtaatgg
    64381 cagaattctg ttcccctgat atgccggtta cgctagtagg agacaatggt gtgacgaagg
    64441 caacgaccgt tcgtgcactg ctgccttatg catttactga aaaagatcta taactcatgt
    64501 tcattcagtc agctgactgt tgaaaaataa aacaattatt ttaggaggct ttacagaaat
    64561 gaaaaaagca aaattatttg gtttaggtgc tgtcgcacta gcagcagggt tatttttagg
    64621 tgcatgcggc aacaacggct ctactgattc atccggtggc aaaagctcat cagacacaac
    64681 aactgcagca ttgatcacag acacaggtgg tgtagacgac cgttcattca accagtcagc
    64741 ttgggaagga ctagaaaaat gggggaaaga tcaaggactt tcacgaggaa atgatggctt
    64801 ccaatatttc caatcttcaa atgaatcaga ttacatccca aatattgacc aagcattgaa
    64861 tgctggattc aaaacgattt ttggtattgg ttataaacta aaaccagcga tccaagaaca
    64921 agcaacaaac aatacaggta cgaactttgt tattatcgat gatgtcatcg atggattaga
    64981 taacgttgta tctgcaactt tcaaagacaa cgaagcttct tatcttgcag gtgttgcagc
    65041 agcttatacg actgaaacaa acgtagtcgg ctttatcggc ggtgtaaaag gtgaagtaat
    65101 cgatcgtttt gatgctggat tcaaagccgg tgttgatgca ggtgcgaaag aattaggaaa
    65161 agaaatcaaa gtattgaatc aatatgctgg tgacttctct gctccagata aaggacgttc
    65221 aattgctcaa ggtatgtatg cacaaaacgc agatatcatc ttccatgctt caggcggtac
    65281 aggtaacggt gtattccaag aagcaaaatc attgaacgaa tcaggcgaca aaaaagtttg
    65341 ggttatcggt gttgaccgcg accaatcaga cgaaggcgaa tacacattaa acggcgaaaa
    65401 gaaaaacttc acattgacat ctacactgaa agcagttgga acagtagtag aagatttggc
    65461 acaaaaatca gcggacggta aattccctgg cggcgaacac actgtttatg gattgaaaga
    65521 agacggtgtt ggtctaacag aaggacaact ttctgacgaa gcgaaaaaag cagttgatga
    65581 agcaaaagaa aaaatcattt caggtgacgt aaaagttcca gaaactcctg aagaaaacta
    65641 aaaccatttc tatagccttg ttagacacat gtttgacaag gctatggttt gattagaaca
    65701 agaattgttc atttatccaa gctgccttgt gacagtttgg ataagggaac aactccctga
    65761 agtaatgttt gtttcaaaag aagaacgctc actaagcgtg aatcttagtc aatcgttgcc
    65821 ctagatagaa atcgttagga tgtgaaaaaa aggtggttca agaaaatatt gtcatagaaa
    65881 tgcagaatat taccaaagaa tttggaacat tcaaggcaaa tgacaacatc aatttacaag
    65941 taaaggcagg agaaattcat gcgttgcttg gagaaaacgg tgctggcaaa tctacattga
    66001 tgaacgtgct ttccggatta ttagagccga catcagggaa aattttgatg cgtgggaaag
    66061 aagtaaagat cacaagtccg accaaagcca accaattagg gattgggatg gtccatcagc
    66121 actttatgct tgttgatgcc tttactgtaa cagaaaacat cgtgttggga agcgagccta
    66181 gtcgcgcagg gatgcttgac cataaaaaag cacgaaaaga gatccaaaaa gtttctgaac
    66241 agtatggatt gtcagtcaac ccagatgctt acgttcgtga tatttcagtt ggaatggaac
    66301 aacgggtaga aattttaaaa acactttacc gaggagcaga tgtactgatt tttgatgagc
    66361 cgacagctgt attgacccct caagaaattg atgaattaat cgtgatcatg aaggaattag
    66421 tcaaagaagg caagtcgatc attttgatta cgcataagtt agatgaaatc aaagcagtag
    66481 ctgaccgctg tacagttatc cgccgtggaa aaggaatcgg tacagtcaac gttaaagacg
    66541 taacctcaca acaattagcc gatatgatgg ttggaagaac ggtttcattc aaaacgatga
    66601 aaaaagaagc gaagcctcaa gaagtcgttt tgtctattga aaatctagtg gtgaaagaaa
    66661 atcgtggatt agaagccgtg aaaaacttga acttagaggt tcgtgctggc gaagtacttg
    66721 gtatcgctgg aatcgatgga aacgggcagt cggagttgat ccaagctttg actggtttgc
    66781 gaaaggcaga aagcggacat atcaagctaa aaggggaaga catcactaat aaaaaacccc
    66841 gaaaaatcac tgaacatggt gtaggacacg tgccagaaga ccgtcataaa tacgggttag
    66901 tcctagatat gacattatct gaaaatatcg cactacaaac ctatcatcaa aaaccttaca
    66961 gtaaaaacgg catgctgaat tattcagtaa tgaatgaaca tgccagagaa ttgatcgaag
    67021 aatatgatgt tcgaacaacg aatgaacttg ttcccgcaaa agctttatca ggcggaaatc
    67081 agcaaaaagc aattatcgct cgtgaagtcg accgagatcc tgatctgttg attgttgcaa
    67141 atccaactcg tggactggat gtaggtgcga tcgaatttat tcataaacgt ctgatcgaac
    67201 aaagggacaa atacaaagca gtgttactga tcagtttcga attagaagaa attttaaatg
    67261 tttcggatcg tattgctgtt atccatgaag gagaaattgt cggtatcgtt gatccgaaag
    67321 aaacatctga aaatgaatta ggtctattga tggcgggcta taccttagaa gaagcacgaa
    67381 aagagttaga aaagggggct ggcgcacatg aatgatcgca gcgaaaaatt ccgtaattta
    67441 ttagtgcctg tactttcagt tgttttagga ttgatcttag gcgcgatatt gatggctgct
    67501 tttggttttc agcccatcca aggttattct tctatgctag atgcagccct tggttcgcaa
    67561 agagggatag gtgaaacgtt aaggcaagca acgccgttga tttttacagc attaggtttt
    67621 tctgtagcta actcagctgg gtttttcaat attgggcttt ctggacaagc actttgcggg
    67681 tggatcgtca gtgtctgggt ggcacttgcg tttccagatc tgccaaaagt tgttttgctt
    67741 ccgttatgcg tgatcttagg tgccttagct ggtgcgatgg cagcggctgt tccaggttta
    67801 cttcgagcat tttttggaac aagtgaagtg atcgtaacaa tcatgatgaa ctatatctta
    67861 ttatacgtaa gcacatttgc tttacatgaa gtgatgcctg aaagctatcg ctcaagtttg
    67921 gactcttcca atatgatcag tcaaaatgcc tcactgcggt taccatggtt aacacaagct
    67981 tttggcggct ctcgcgtaaa ctcaggatta tttttagcaa tcatcgcgct tattttcgtt
    68041 tggctgctga tgaagaaaac gactttagga tttgaaatcc gttctgttgg attgaatcct
    68101 tttgcttctg agtatgcagg aatgagtagt aaacgtacga tcgttttgtc gatggtcatt
    68161 tctggcgctt tggcagggct tggcggtgtg gttgaaggat taggaacgta tcaaaacttc
    68221 tttgttcaaa cgacttcgct atctattgga tttgacggaa tggctgtttc cttgcttggt
    68281 tctggatcag caatcggtat tcttttatca gcgatcttgt tcagtatttt gaagatcggt
    68341 ggacttggca tgcagacagg agcaggtgtt ccgtttgaga tcgtcaatgt atcgattgct
    68401 ttgattatct tctttgtcgg gatcaacttc ctgattcgtt tcgtcatggc taaagtcttc
    68461 caagggaaaa aacaagaaga gatcgttgcg acaatagaat cgaaaccaac gaatcaaaac
    68521 catgaagggg gcgaactata atgtcgacta tcgaattaat tgccttgatc cttacttcta
    68581 cactggttta ttcaacaccg ctaattctaa cgtcactagg cggaactttt tctgaacgaa
    68641 gcgggatcgt aaacgtaggg ctagagggaa tcatggtcat gggtgctttt agttccatcg
    68701 ttttcaactt gacgatgagt ccatcacttg gttctatgac accatggatc gctattttag
    68761 tgggtggcgt cgtaggtatc ctattttctc tgattcacgc agtagcaaca gtcagtttgc
    68821 gtgctgacca tattatcagc gggacagtaa ttaatctgat ggcacctgct ttaggtgttt
    68881 tcttgatcaa agcatggtat ggtaaaggtc aaactgataa catcacgcag aatctggggt
    68941 atttttcttt cccaggatta gcagatattc cggtcattgg accaattttc tttaaaaata
    69001 cattcttacc tgcttattgt gctattttga ttgctgtcct agcttgggtg atcatcttca
    69061 agacgaaatt cggcttgcgc ctccgttcag ttggtgaaaa tcctcaagct gccgacacat
    69121 taggtatcaa tgtatatgct atgcgttatt caggcgtact gatttctggc ttgttaggcg
    69181 gtatgggcgg tgctgttttt gctcagtcga tttccggtaa cttttcagct ggtacgatcg
    69241 ttggacaagg gttcatctcc atggctgcta tgatctttgg taagtggaat cctttagggg
    69301 caatgggtgc ctcattgttc tttggttttg ctcaaagctt gagtatcatt ggcgcacaat
    69361 taccagttat ttcttcgatt ccgccagtat tgctgcaaat tgctccatat ttgctgacaa
    69421 tcattgtgct ggttgttttc ttagggaaag cttctggtcc aaaagcaaac gggaagaact
    69481 atatcaaatc aaaataatct tttagcgaat aaaaagggtg ggttacggga agtgtttttc
    69541 gtctcgccct ttttgtttta aaggcaataa aaataaaaag aaagaaggaa taatcatgtt
    69601 taaacgcgta catctgatcg tcatggattc agtaggaatt ggagaagcac cagatgccga
    69661 taagttcggt gatgttggtt ctgatacatt aggacacatt gcgaaagaag caggattgaa
    69721 cgttccacat ttagagcagc ttggtttagg aacgatccgt cctcttgaag gtgtggaatc
    69781 agtgaaagat catcaaggct atgcgacgaa attagaagaa gtttctgtag ggaaagatac
    69841 gatgacaggc cactgggaaa tcatgggatt gaatatcaaa actccatttc gtgtgtttcc
    69901 taatggattc cctgatgatc tgatcaaaca aatcgaagaa ttttctggcc gtaaagtcgt
    69961 atgtaacaaa ccttatagcg gaacagctgt tattgacgat tacggtgaac accaaatgaa
    70021 aacaggcgat ttaattgtat atacttctgc cgatcctgtc cttcagatcg cggcacatga
    70081 agaaatcatt cctttagaag aattatatcg gatttgccaa ttcgttcgtg atataacaaa
    70141 agatgatccg tatatgattg gccgaatcat tgcacgtccg tacgtgggtg aaccaggaga
    70201 cttcacacga acaagcaatc gccatgatta tgcgttgaat ccattcggac acacagtcct
    70261 tgattctttg aaagaagcag gaaaggacgt gattgctgtt ggaaagatca acgatatttt
    70321 caacggacaa ggaatcactg aatctgtccg cacgaaaagc aatatggatg gcgttgacca
    70381 gctgctgaaa gttatgaaaa acgatttttc aggaatcagc tttaccaatt tagtagattt
    70441 tgatgcatta tacggtcatc gtcgggatgt agtcggctat gcgcatgcga ttgaagaatt
    70501 tgacttacga attccagaat tgcttgaagc gatggaagaa gacgacttgc taatgattac
    70561 agccgatcac ggaaacgatc cgacgtttac tggtaccgat catacgcgag aatatgtacc
    70621 gttattggtt tacagtaaaa aaatgaaaga acaaggggca ttgcctcaag gatattattc
    70681 agatatttca gcaacgattg ctgagaactt tagtgtttct tcgactgaaa atggcgaaag
    70741 cttcttgaaa ttattaaaat aatcttttta aataggagaa agcaaatgac caagttaagc
    70801 gagatgttac aagaaacgac ccagtttatc cttgataaag gagtaaaaga aattgatttt
    70861 ggtttgattc taggttcagg attaggtgaa ttagcagatg aaatcgaaga tgctgtaatg
    70921 atttcttatg atgagatccc gttctttcct gtttcgacag tagttgggca tgcaggaaag
    70981 ctagtttatg ggtctctggc aggcaagaaa gtgttagcca tgcaagggag attccactac
    71041 tatgaaggtc attccatgca aacagtgacc tatccagtac gtgtgatggc cggactaggg
    71101 gcccattcag tcattgtgac taatgcatgt ggtggtgtca acgaatcatt tgtaccagga
    71161 gatttgatgc tgattaccga tcatatcaat tttactggtg aaaatccttt gatcggtccg
    71221 aatgaagatg caatgggccc tagatttcca gatatgagtc aagcttatac gcctgactat
    71281 caagcaacag caaaagcagc agcagaaaaa ctaggtttgg atcttaaaga aggcgtatat
    71341 atgggctatt ctggaccaac ttatgaaacg cctgcggaga tccgaatgtc tcggacaata
    71401 ggagccgatg cagtagggat gtcgactgtt ccagaagtca tcgtagcagc tcacagcgga
    71461 ttaaaagtgt taggaatctc ttgtattacc aacttagcag caggtatgca agcgaatctg
    71521 agccatgaag aagttgtaga aacaactcag agagtcaaac aatcttttaa agcattggtt
    71581 aaagaaacat tgacattatt gtaataggag gaacaataaa atgagtgtgc atattgaagc
    71641 gaaagaaggc gagatcgccg ataagatttt acttccggga gatcctttgc gagctaaata
    71701 tattgcggag acttttttgg aagatccagt ttgttacaat caggtacgcg gtatgttagg
    71761 ctttaccggc aaatataaag gagaacgtat ttctgttcaa ggaacaggaa tgggaatgcc
    71821 atcagccacc atctatgccc atgaattgat ccagtcgtat ggtgtaaaga aattgattcg
    71881 agtaggtaca tgcggtgcat tgtcaaaaga cgtgcatgtt cgtgatttag tactagcaca
    71941 aggtgcagcg accagttctt cgatgatcga aaaaaatttc caagcctttc attttcctcc
    72001 aatcagtgat ttcaatctat tactgaaagc atatgagata gctaaagaaa agggctatac
    72061 ggttcatgta ggaaatgttc tttcggaaga ttctttctac aaagatgatt tgacagagac
    72121 gttccaatta gctgaacttg gcgttttagg agtggaaatg gaagcagcgg ctctttatta
    72181 cttaggcgcg aaataccatg tgcagacatt aagtttgatg accgttagcg accatttgat
    72241 caccggtgaa gaaacaacag ctgcagaacg tcaatctacg ttcaatgaaa tgatcgaagt
    72301 aggattggaa acagcgatag cagaataaga aggaaatgaa aaagctgccc tgcattaagt
    72361 aataagaaca tccaagcaaa aatagctcct catgtttttg acttggctgg acttattatt
    72421 gcaaatgtaa gcttttgaac accgtttacc aagaggttgt gaatctgccg tttaactctg
    72481 agtcacaagg aacaatttca aaaaacagct tttcatgttt gatgaatatt gtgacttgtg
    72541 accgagaagt tggcaggaga aaaccgttta tacgaaaaaa gggactgtga caaatcgtgt
    72601 cacagtccct tttttatagt gttttttata ggtagtattt tttagcaact gttaagtcat
    72661 catttaattc ataaacaagt ggttgacctg ttggaatttc aagatccatg atgtcttcgt
    72721 cagagatacc ttcgatatgt ttagctaggg cgcgtaatga gttaccatgt gcagctacta
    72781 aaacagtttt gttgtctagt aatgctggag caatttcatc ttgccagaat ggcaaagcgc
    72841 gttctaatgt cactttcaag ttttctccac caggaatatc gcgtttgtct aacattgcgt
    72901 aacgacggtc atttgctgca gaaccttcgt cagtttcttc cattaatgga ggaagagtat
    72961 cgtaagaacg acgccagatg tgcacttgtt cgtcgccgta tttttcagct gtttcttttt
    73021 tgttcaaacc ttgtaattta ccatagtgac gttcgtttaa gcgccatgat ttgatttgtg
    73081 gtacccaaag ttgatctgaa tattctaaga tcaagttaca tgttttgatt gcacgagtta
    73141 aaacagaagt gtaagcaaca tcaaattcga ttccagcttc tttgattttg cgtccgcctt
    73201 cttttgcttc ttcgattcct tctggtgcta gatttacatc agcccaacca gtaaattggt
    73261 tcaatgcatt ccattcacta agaccgtgac gagaaaaaac taattttggc atgtttagct
    73321 ctccttttaa gataggttta tatttctgaa attttcagtt ctgttttatt gtacaatttt
    73381 ttggaagaga tttccatcga aaaattagat aagattctat gagaaaacaa gtagagaata
    73441 taaaaaaacg aacagtgccc acgcaactgt tcgtttttag gtattagatg cataattgct
    73501 cgatttcttt ttcaggtaaa ttgatatatt tataacatgt tccaactgaa acaccgcatt
    73561 ttgaagaaat aaattggatc gtttcttttt tttcataata gagacgtcgg atttttttga
    73621 tcgtttttgc atcgattttt ggtcttccgc cgatttttcc ttttttacga gcttcatcta
    73681 atccaactaa tgtacgttct ttgatcaatg cgcactccat gtttgctagg ttttccatta
    73741 attggaaata gatttttccc atttcgcgcg tatcgattcc ttcatcaaga ctaatcaagt
    73801 gcagcccgcg atctttgaat aattgcgtga gttctgttaa ttgccgcgta gatttgccta
    73861 atcgatgaag ttggcaaatg actagtgcat cgccttgttg catgtcagcg atcactgact
    73921 ccaaatgaac agcagtagaa tcattttgtt ccacttcata tgtctcatgg aaaatttctt
    73981 gacaaccaaa gtcagataat tgatcaacct gagttgttaa atcaacgtca tttatggttg
    74041 tgcgtgcata accaataatt ttcatggtga ttcctttcta gcagtgattt tctaactgga
    74101 tataacagta aaaatgtagc ataaagtacg tcaaaaagat agttttttcg caaattttcc
    74161 gctgtctttt tttattggat gatcaagcag tttttcttta tttacgacgg attgcgtcaa
    74221 gaatctcaac gcctttttct gagccgacaa ctactttaga aaccatgttt aggaaaagcc
    74281 catgttctac taccccaaca agttcatcta aataggtagc taagctttct ggctgttcga
    74341 tgacttctag atgcagatca atgatgtaat gaccaccatc tgtggttaag acttctccgt
    74401 tttcgtctgt acgcaagacg ggttggaatc ttttttcttc aaaaatatga agtagctgtt
    74461 gggagccata agggatcact tcaactggga gtgggaactt cccaagttta tcgactaatt
    74521 tagaagaatc aacgatccaa atcgtttctt ttgagtacgt agcgactatt ttctcgaaaa
    74581 gcaaagcagc gccgccgcct ttgatccctt gaaaaccagc actgatttca tcagcaccgt
    74641 ctattgtcaa atcgactacg gggacttcat caatactctt taacggtatt cctaattttt
    74701 ccgcttgttc ttttgtcgca tttgaagtag taactcctgt gatacgtaaa ccttcttcac
    74761 gcatacggcg accaatctct tctaccatat aataggcggt tgaaccggtc ccgagaccta
    74821 cgatcatccc gtcctttacg aatttcgcgg cttcaatgcc agctaattgt tttaaattca
    74881 tctttgcgtc cacctttttt tagtttgagc atcgtgcttt cctcactatt ttagcgtgat
    74941 ttacggtaaa aagataccat tcttttttat tccaataaag ctttttcaaa aaaaattgca
    75001 tctcttgttg acatgttttt tcattcgtga tattctaaca aaggtgcttt atctacaggt
    75061 ttctgtgaag aaacgtgctg actgtttatc aaagcatatt tgataaggat gcagacattg
    75121 catttatttt ttatcacaaa aaaagaacca cctggatgtg tggaactaga cgcaaatcgt
    75181 gggaaggagg aaacaaggaa tgcctacaat taaccaatta gtacgtaaac ctcgtaaatc
    75241 aaaggtggaa aaatctaatt cacctgcttt gaacaaagga tataacagct ttaagaaaac
    75301 tcaaactaac gtaaactcac ctcaaaaacg tggggtagca actcgtgtgg gtacaatgac
    75361 acctaaaaaa ccgaactcag ctttacgtaa atatgcacgt gttcgtttgt caaacttaat
    75421 tgaagttact gcttatatcc cagggattgg tcataactta caagaacaca gtgttgtatt
    75481 attacgcggt ggtcgtgtaa aagacttacc aggggtacgt taccatatcg ttcgcggtgc
    75541 tttagatact gctggtgtta acgaccgtaa acaaagccgt tctaaatacg gaactaaaaa
    75601 acctaaagct taattttaat tattaaacaa atgaaacttg cttctactat atagaagtaa
    75661 ttactatgaa caatatttcg gaaggaggag ttacggatgc cacgtaaagg tcctgttgca
    75721 aaacgtgatg ttttaccaga tcctatttat aactcaaaat tagtaactcg cttgatcaac
    75781 cgtgtaatgg ttgacggaaa acgcgggatt gcagctaaca ttatctataa tgcctttgac
    75841 ttgatcaaag aatctacagg taacgatcca ttagaagtgt ttgaacaagc aatgaaaaac
    75901 gttcaacctg ttcttgaagt taaagcacgt cgtgttgggg gttcaaacta ccaagtacca
    75961 gttgaagttc gccctgaacg ccgcgttact ctaggtcttc gttgggtagt aaactacgct
    76021 cgcttgcgcg gtgaacacac aatggaagaa cgcttagcaa aagaaatcat ggatgctgca
    76081 aacaacacag gtgcttctgt taaaaaacgt gaagacactc ataaaatggc tgatgccaac
    76141 cgtgcgtttg ctcactatcg ctggtaagat tctctctgtt tggtcggctt ttataagtcg
    76201 acctcagata gtgtccttat ttttagcaac tagatagtaa agagaggaga aacaaggaga
    76261 atgaccagag aattttcttt acaaaacact cgtaatatcg gtatcatggc ccacatcgat
    76321 gctggtaaaa cgactgctac tgaacgtatc ttgtactata ctggtaaaat ccataaaatt
    76381 ggtgaaactc atgaaggagc ttcacaaatg gactggatgg aacaagaaca agaacgtggt
    76441 attacgatca cttctgctgc tactacagca caatggaagg gtcaccgaat caacatcatc
    76501 gacacaccag gacacgtaga cttcacagtt gaagttgaac gttcattgcg tgtacttgat
    76561 ggtgctgtaa ctgttcttga tgcacaatca ggtgttgaac ctcaaactga aacagtttgg
    76621 cgtcaagcaa caacttatgg tgtacctcgt atcgtatttg caaacaaaat ggacaagatc
    76681 ggtgctgact tcttatattc agtatcaact ttacatgacc gtttgcaagc aaacgctcac
    76741 ccaatccaat taccaattgg tgcggaagac gacttcacag gaattatcga ccttgtaaca
    76801 atgaaggctg aaatgtatac aaacgatctt ggaacagaga tcgaagaaac agaaattcct
    76861 gaagaatacc gcgaattagc tgaagaatgg cgcgaaaaat tggttgaagc tgtagctgaa
    76921 actgatgaag aacttacttt gaaatactta gaaggtgaag aaatcactga agctgagtta
    76981 aaagaaggta tccgccgcgc aacagttaac gtagaattct acccagtact atgtggttca
    77041 gcatttaaga acaaaggggt tcaattacta ttggacgctg ttcttgatta cttgccatca
    77101 ccgttagata tcccagctat caaagggatt gaccctaaaa ctgatgaaga agtagaacgt
    77161 cctgcagacg acagcgctcc tttctcagct ttagcattta aagttatgac tgacccattc
    77221 gtaggtcgtt taactttctt ccgtgtgtac tctggtgttt tacaatcagg ttcatacgta
    77281 caaaacgcaa caaaaggcaa acgtgaacgt gttggtcgta tcctacagat gcatgccaac
    77341 tctcgttcag aaatctctga agtttacgct ggagatatcg ctgctgctgt tggtttgaaa
    77401 gacacaacta ctggggacac actatgtgat gaaaagagtc tagttattct tgaatccatg
    77461 gaattcccag aaccagttat ccaagtcgct attgaaccta aatcaaaagc tgaccaagat
    77521 aaaatgggtg tggctttaca aaaactatct gaagaagatc ctactttccg tgctgaaaca
    77581 aacgtggaaa caggcgaaac aatcatcgct ggtatgggtg aacttcactt agatatcatc
    77641 gttgaccgta tgcgtcgtga attcaaagtt gaagctaacg taggtgcgcc tcaagtatct
    77701 taccgtgaaa ctttccgtgc tggcactcaa gccgaaggta agtttgtacg tcagtctggt
    77761 ggtaaaggtc aatacggtca cgtatggatt gaatttacac caaatgaaga aggtgctggt
    77821 ttcgaatttg aaaatgcgat cgtcggtggg gttgttcctc gtgaatacat tccagctgtt
    77881 gaaactggtt tgaaagatgc tatggaaaat ggtgtcttag ctggctatcc attagtagat
    77941 atcaaagcta aactttacga tggttcatac catgatgtcg attcaaatga aactgccttc
    78001 cgtgttgctg cttctatggc attacgtgca gcagctaaga aagctaatcc agtaatcctt
    78061 gaaccaatca tggctgtaga agttgttatc cctgaagatt atttaggtga tgttatgggt
    78121 cacgtaactg ctcgtcgtgg acgtgttgaa ggtatggaag ctcgcgctgg cggccaacaa
    78181 gttgttcgtg cgatggttcc acttgctgaa atgtttggtt atgctacaac tcttcgttct
    78241 gctactcaag gtcgtggtac atttacaatg acatttgacc actacgaaga tgtaccaaaa
    78301 tcagtacaag aagaaatcat taagaaaaac ggcggaaaag ctgaataatt tcttaaaacc
    78361 ggatagtcct gcaaagagat tatccaaaga gactatattt ttaatatagt ttcgtgtaaa
    78421 ataattatcg atgatatggg catgtggtgt accttgtgta catcatatac ctatcagaca
    78481 aataaaaaat atattgtatt tttaggagga cattttaaaa tggcaaaaga aaaatttgac
    78541 cgttctaaac cacacgttaa cattggtact atcggacacg ttgaccatgg taaaactaca
    78601 ttaacagctg caatcacaac tgtactatct aagaaaaacg gcggccaagc tatggcttac
    78661 gatcaaatcg atggtgctcc agaagaacgc gaacgcggaa tcacaatctc aacagctcac
    78721 gttgagtatg aaacagatac acgccactac gctcacgttg actgcccagg acacgcggat
    78781 tacgttaaaa acatgatcac tggtgctgct caaatggacg gagctatctt ggtagtttct
    78841 gctgctgacg gcccaatgcc tcaaactcgt gaacacatcc tattgtctcg tcaagttggt
    78901 gttccttaca tcgttgtatt cttgaacaaa gtagacatgg ttgatgacga agaattacta
    78961 gaattagttg aaatggaagt tcgtgaccta ttaacagaat acgaattccc tggtgacgat
    79021 gttcctgtag ttgctggatc agctttgaaa gctctagaag gcgacgcttc atacgaagaa
    79081 aaaattcttg aattaatggc tgcagttgac gaatacatcc caactccaga acgtgacaac
    79141 gacaaaccat tcatgatgcc agttgaagac gtgttctcaa ttactggacg tggtactgtt
    79201 gctacaggtc gtgttgaacg tggacaagtt cgcgttggtg acgaagttga agttgttggt
    79261 attgctgaag aaacttcaaa aacaacagtt actggtgttg aaatgttccg taaattgtta
    79321 gactacgctg aagctggaga caacattggt gctttactac gtggtgttgc acgtgaagac
    79381 atccaacgtg gacaagtttt agctaaacca ggtacaatca cacctcatac aaaattctct
    79441 gcagaagtat acgtgttgac aaaagaagaa ggtggacgtc atactccatt cttcactaac
    79501 taccgtccac aattctactt ccgtacaact gacgtaacag gtgttgttga attaccagaa
    79561 ggaactgaaa tggttatgcc tggcgacaac gtaacaatgg aagttgaatt aatccaccca
    79621 atcgctatcg aaaacggtac taaattctca atccgtgaag gcggacgtac agttggtgcc
    79681 ggtgtcgtta cagaaatcaa agcttaattc acttaagttt taaagtaaag caacatttat
    79741 tcggacgtaa tcgctcggaa cgaaggtctt aggatctttg ttccgagctt ttttatttat
    79801 tgcaggatca ggaaaaaaga caggagtatt ataaatagaa gaaacacgaa ataaaagagt
    79861 cagtattttc taaatgaaac tttatgaaaa caaaatgttt tttacaagaa aaagccttgc
    79921 tttgtgagtt tatatatatt ataatgacaa aggtgcgttt actttagtga attgcacaga
    79981 cgtaatcaga atgatttcgt cggctaagaa acgagaggtt gcgacacgcc cggacgcttt
    80041 gccacgaacg agcgtgacgg aaattttcgt ggagctatgt ctacttttca aaataggcga
    80101 aggagggaac aagatggcaa aacaaaaaat tcgtatccgt ttaaaagcgt atgaacaccg
    80161 tattttagat caatcagcgg ataaaatcgt ggaaactgca aaaagaactg gagctagcgt
    80221 gtctggtcca attccattac caactgaacg cagtctttac acaatcatcc gtgcgactca
    80281 taaagacaaa gattcacgcg aacaattcga aatgcgtaca cacaaacgtc ttatcgacat
    80341 tgtgaaccca acaccaaaaa ctgttgatgc tttgatgaag ctagacttac catctggtgt
    80401 aaacatcgaa atcaaattat aaaactaaaa caaaaataat ggaggtgtac tcatgaccaa
    80461 aggaatctta gggaaaaaag tgggaatgac acaaatcttc actgaatctg gtgaattaat
    80521 tccagtaact gttgtagaag ctactccaaa cgtagtacta caagtgaaaa caatggaaac
    80581 ggatggctac gaagctattc aagttggtta ccaagatatg cgtgaagttt tatcaaacaa
    80641 acctgcgaaa ggtcatgttg caaaagcaaa cacggctcct aagcgcttca ttcgtgaatt
    80701 caaaaatgtt gagctaggag aatatgaagt aggaaaagaa attacagtag atgttttcca
    80761 agcaggagac attattgatg taacaggtac tacgaaaggt aaaggattcc aaggggttat
    80821 caaacgccac ggacaaagcc gcggaccaat ggctcacggt tctcgttacc atcgtcgtcc
    80881 tgggtcaatg ggtccagttg cacctaaccg tgtatttaaa aacaaacgtc ttgctggccg
    80941 tatgggtggc aaccgcgtaa ctatccaaaa cttggaagtt gtacgtgtcg atgcagaaag
    81001 aaatgttatc ttgatcaaag gtaatgttcc tggagctaaa aaatctttaa ttactatcaa
    81061 atcagctgtg aaagctaaat aattgtagga agaaaggagg aactaaggaa tgccgaatgt
    81121 agcattattc aaacaagatg gaagccaaaa cggtgaaatc acacttaacg aagaaatctt
    81181 cggaattgaa cctaatgaaa gtgttgttta cgatgcaatc gtaatgcaac gtgcgtcatt
    81241 aagacaagga acacacgcgg ttaaaaaccg tagcgctgtt cgcggcggcg gacgtaaacc
    81301 atggcgtcaa aaaggaacag gtcgtgcacg tcaaggatct atccgctcac cacaatggcg
    81361 tggaggtggc gtagtctttg gaccaacacc acgttcttac agctacaaac ttcctaaaaa
    81421 agttcgtcgt ttagcaatga aatctgtatt gtcagaaaaa gttgctgaaa acaacttggt
    81481 agcaatcgaa ggcttgaact tcgacgcacc aaaaacaaaa gaattcaaac aagttcttgc
    81541 taacttatct atcgactcta aagtattggt tgttctagaa aaaggtaacg actttgctgc
    81601 tttatcagca cgtaacttac caaacgtttc tgtagtaact tctgataatg tgagtgtgct
    81661 agacgttgta tcaaatacaa aagtgttagc aacacaaact gctcttactc aaattgagga
    81721 ggtgcttgca taatgaactt actagacgta atcaaacgcc cagtgatcac tgaaaaatca
    81781 atgcttgcca tggatgacaa gaaatacact tttgaagtgg acactcgcgc aaacaaaact
    81841 ttagtaaaac aagcggtaga agctgctttt gacgttaaag tcaaaaacgt gaacatcgtg
    81901 aacgtacgtg cgaaattcaa acgtatgggc aaatatgcag gatatacaaa aaaacgtcgc
    81961 aaagcgatcg ttacattgac tgaagattca aaagaaatcc aattattcga agctgctgaa
    82021 taagcactaa tagataactc attaaattag gagggaaaaa cgtggcgatt aaaaagtaca
    82081 aacctacctc aaatggtcgt cgtaacatga caggttctga ttttgctgaa atcacaacat
    82141 caacacctga aaaatcattg ttacaaccat tgaaaaacca tgctggacgt aacaacaacg
    82201 gccgcatcac tgtacgtcat caaggtggcg gacacaaacg ccaatatcgc gtgatcgact
    82261 tcaaacgtaa caaagataac gttgttgcaa ctgttcaaac gatcgaatat gatccaaacc
    82321 gttcagctaa catcgcgtta gtacactatg aagatggagt gaaagcttac atcttagcac
    82381 ctaaaggact agaagtgggt atgaaacttg tttcaggtcc agaagcagat atcaaagtcg
    82441 gaaatgctct tccattagaa aacattccag ttggtactgt tatccacaac attgaaatga
    82501 aacctggtaa aggtggacaa ttgatccgtt ctgctggtac aagtgctcaa gtacttggta
    82561 aagaaggcaa atacgtattg atccgcttga actctggtga agttcgtatg atcttagctg
    82621 catgccgtgc tacaatcggt tctgtaggta acgaacaaca cgaattaatc aacatcggta
    82681 aagctggacg ctctcgttgg atgcgtaaac gcccaactgt acgtggtagc gtaatgaacc
    82741 ctaatgatca cccacacggt ggtggtgaag gtaaagctcc tatcggacgt aaagcaccag
    82801 tttctccttg gggtcaacca gctcttggtt acaaaactcg taacaaaaaa gctaaatcag
    82861 acaaacttat tgttcgtcgt cgcaaaaata aataattact ttgaccatgt gtcgcacatt
    82921 ttgagaggag gttcaccatg ggtcgtagtt taaagaaagg accttttgtc gatgagcatt
    82981 taatgaaaaa agttgaagct caagcagggg ttgaaaagaa aaaagttatc aaaacttggt
    83041 cacgccgttc aacagttttc ccaagttttg ttggatatac catcgcagtt tatgatggac
    83101 gtaaacatgt accagtttac atccaagaag acatggtagg acacaaatta ggtgaatttg
    83161 cacctacaag aacttatcgt ggacatgctg ctgacgataa gaaaacgaaa cgctaatttg
    83221 aggggaggat aaatccaaat ggcagaacaa atcacatcag ctaaagcaac tgcaaaaaca
    83281 gttcgcactt cacctcgaaa agcccgttta gtaattgacc ttatcagagg taaaagtgtc
    83341 gcagatgcaa tttcaatctt gaaattcaca ccaaacaaat ctgctggaat cattgaaaaa
    83401 gtattgatgt cagcagttgc gaatgcagaa aataactttg acttagatgt tgaaaacttg
    83461 gtagtaagcg aagcttttgt taacgaagga cctacaatga aacgtttccg tcctcgtgca
    83521 aaaggatcag cttctccaat caacaaacgt acaagtcata tcacggtagt cgtatcagaa
    83581 aaataaggag ggataatcag tgggtcaaaa agtacatcca attggaatgc gtgtaggcat
    83641 catccgcgac tgggatgcta aatggtatgc tgaaaaagag tatgctgagt tcttacacga
    83701 agatttaaga atccgtaaat ttatttcaac taaacttgct gatgctgctg tatcaacagt
    83761 tgaaatcgaa cgcgcaacag gtcgcgtaaa catttcaatt cacacagcta aaccaggtat
    83821 ggttattggt aaaggcggtt ctgaagtcga aagcctaaga aaagaattaa acaaattaac
    83881 tggtaaaaga atccatatca atatcgtgga aatcaaaaaa ccagatctag atgcaaaatt
    83941 agtaggtgaa ggaatcgcac gtcaattaga aaaccgtgtt gctttccgtc gcgctcaaaa
    84001 acaagccatc caacgctcaa tgcgtgctgg cgctcaaggg atcaaaactc aagtatctgg
    84061 tcgtctaaac ggagcagata tcgctcgttc agaaggatac tcagaaggaa ctgttcctct
    84121 tcatacttta cgtgcggaca ttgattacgc atgggaagaa gcagatacaa catacggaaa
    84181 actaggagtt aaagtgtgga tctatcgtgg agaaattctt ccaactaaaa aaaacactga
    84241 gaaaggaggg aaataatcat gttagtacct aaacgtgtaa aacaccgtcg tgaattccgc
    84301 ggaaaaatgc gcggggaagc taaaggcgga aaagaagtag cattcggtga atacggtttg
    84361 caagctgttg attcacattg gatcacaaac cgccaaatcg aagctgctcg tatcgcaatg
    84421 actcgttaca tgaaacgtgg tgggaaagta tggattaaaa ttttccctca caaatcttat
    84481 actgccaaag caattggggt acgtatgggt tctggtaaag gggcacctga aggatgggtt
    84541 gcaccagtaa aacgtggtaa aatcatgttt gaaatcgcag gcgttcctga agaagtagct
    84601 cgtgaagcgt tacgtctagc ttctcacaaa ttaccaatga aaactaagat cgtaaaacgt
    84661 gaggaaatgg gtggtgaatc gaatgaaggt taaagaaatc agagaattaa ccactgccga
    84721 aatgcttgat caagaaaaac aattaaaaga agaattgttt aatcttagat tccaattagc
    84781 aacaggtcaa ttagaaaaca ctgcacgtat taaagaagta cgtaaatcga ttgcacgcat
    84841 caaaactgtg ttgcgtgaac aagctaagta attatggaag gaggccatta agcgtatgac
    84901 tgaaggaaga aatcaacgca aagtttatca aggtcgcgtg gtttcagaca aaatggataa
    84961 aacaattacc gttgctgtcg aaacaaagaa aaaccaccct atctatggca aacgcatgaa
    85021 ctattctaag aaatacaaag tgcatgatga aaacaacaca gcaaaagttg gcgacatcgt
    85081 gagaattatg gaaactcgtc cattatcagc tacaaaacgt ttccgtttgt tggaagtagt
    85141 cgaagaagca gttattatct aataacacga gattttccta tatagattga atgcaaaatc
    85201 tgaaaggagg atacacaacg tgatccaagc agaaagtcgt ttaaaagtcg ctgataactc
    85261 aggcgcacgt gaaattttga cgatcaaagt ccttggtgga tctggtcgta aaactgctaa
    85321 catcggtgat gtgatcgttg ctacggttaa acaagcaacg ccaggtgggg ttgtcaaaaa
    85381 aggtgacgtc gttaaagccg ttatcgttcg aacaaaatca ggagctcgtc gtacagacgg
    85441 ttcatatatt aaatttgatg aaaatgctgc agtgattatt cgtgatgata aaagccctcg
    85501 tggaacacgt atctttgggc cagttgcacg tgaactacgt gaaaacaact tcatgaagat
    85561 cgtttcacta gcaccagaag ttttataatc ttgagacact tatcaaagga ggtgcgaaac
    85621 agttatgttt gttaaaaaag gcgataaagt taaagttatc actggaaaag acaaaaacaa
    85681 agaaggcgtt gtactagcag cgttcccgaa aaaagataaa gttattgtcg aaggtgtaaa
    85741 tgtcgttaaa aaacaccaaa aaccttctca agctgctcct caaggtggaa ttgttgagat
    85801 ggaagcgcca attcatgttt ctaacgtgat ggtggttgac tctactggtg tagctggccg
    85861 agttggctac aaagaagtag acggcaaaaa agtccgtgtt tctaaaaaaa ccggtgaagt
    85921 tttagataaa taaaagttta aggaaggagg gtcttactga atgaaccgcc taaaagaaaa
    85981 atatcttaaa gaagtaactc catcattgat ggaaaaattt aactatagct ctgttatgca
    86041 aacacctaaa gttgaaaaaa tcgtcatcaa catgggtgtt ggtgatgcgg tatcaaacgc
    86101 taaaaactta gataaagcag ttgaagaatt aactttgatc actggtcaaa aaccaatgat
    86161 tacaaaagct aaaaaatcaa tcgctggatt ccgtttacgt gaaggaatgc caattggtgc
    86221 aaaagttaca ttgcgcggag aaagaatgta cgaattctta gacaaattgg tttctgtttc
    86281 actaccacgt gtacgtgact tccatggtgt aagtaaaaaa gcctttgacg gtcgtggaaa
    86341 ctatacttta ggtattaaag aacaattgat cttcccagaa gtagattatg atttagtaga
    86401 taaagtacgt ggtatggaca tcgttattgt aacaacagca aactctgatg aagaatctcg
    86461 tgagttgttg acacaattag gcatgccatt ccaaaaataa ttaaaggagg cgaactacgt
    86521 ggctaaaaaa tcaatgattg ctaaaaacaa acgtcctgca aaattttcaa cacaagctta
    86581 tactcgttgc gaacgttgcg gacgtccaca ttcagtttat cgtaagttcc atctttgccg
    86641 tatttgcttc cgcgaacttg cctataaagg tcaaattccc ggcgtgaaga aagctagctg
    86701 gtaaaaagta aattcgcata agaaggaggt aaaagatcaa tggtcatgac agatccaatt
    86761 gcagattttc taactcgtat ccgtaacgca aacatggtta aacatgagtc tttagaagta
    86821 cctgcatcaa aaattaaacg tgacatcgct gaaatcttga aacgtgaagg tttcgttcgt
    86881 gatgttgaat acatcgaaga tgacaaacaa ggcgtgatcc gtgttttcct aaaatttggt
    86941 aaaaacggcg aacgtgttat cacaaactta aaacgtatct ctaaaccagg tttgcgtgct
    87001 tatgtcaaag ctgacgaagt accaaaagta ttgaacggtt taggtatcgc aatcatttcg
    87061 acttctgaag gtgttgtcac tgataaagaa gctcgtgaaa aaaatatcgg cggcgaagtt
    87121 atcgcttacg tatggtaatc aagtagaatc tacaaggagg tgtctttaag tgagccgtat
    87181 cggaaataaa gtcgttgtta tccctgaagg agtaacagtt actcaagacg gaaacaacgt
    87241 tacagttaaa ggaccaaaag gtgaattgac tcgtactttt tcagcagata tcaaaatgaa
    87301 tatcgaagga aacgaagtaa ctttcactcg tccaaacgac agtaaagaaa tgaaaacaat
    87361 tcatggaaca actcgtgcga acttcaacaa catggttgta ggtgtcagcg aaggtttcca
    87421 aaaagcgtta gaactaatcg gggttgggta ccgtgcacaa ttacaaggat caaaacttgt
    87481 attgaacgtt ggttactcac atccagtaga aatcacacca ccagctggag taactgttga
    87541 agtaccagct aacacacaag taattgttaa aggtgcgaac aaagaagaag ttggcgaatt
    87601 agctgcaaac attcgtggaa ctcgtcctcc agaaccttat aaaggtaaag gtattcgtta
    87661 tgttggcgaa tatgtacgcc gtaaagaagg taaaactggt aaataatcgt ttagaaaaat
    87721 tgcatagaag ccagcagcag gaaaaaggca tttggctgaa gtttaccttc aaaaaaatac
    87781 atttttactt gtgccgcagc ttctattttt cgaaacgtat ttactgcttg aaatagtagt
    87841 acaataaaaa taaagaggtg acaattgtga ttacaaaacc agataagaac aaaacacgtc
    87901 aaaagagaca tcgtcgtgta cgtaacaata tctctggtac tgctgagcgc ccacgcttga
    87961 acgtttttcg ttctaacaaa aacatctacg ctcaaattat tgatgacgta gcgggtgtaa
    88021 cgctagcaag tgcctctacc ttggataaag aaatttcagg tggaacaaaa acagaagcag
    88081 cacaagctgt cggtaaacta gttgctgaac gtgcagctga aaaaggcatt aaagtagtag
    88141 tctttgaccg tggtggatac ctttaccatg gccgtgtgca agctttagct gaagctgctc
    88201 gcgaaaatgg actagaattt tagaagaagg aggaatacca ttcatggttt atatcgatcc
    88261 aaaacatttg gaattagaag accgcgttgt tgcaattaac cgtgtaacaa aagttgttaa
    88321 aggtggacgt cgtctacgtt ttgctgcttt agttgttgtc ggtgacaaaa acggacacgt
    88381 agggtttggt acaggtaaag ctcaagaagt tcctgaagca atccgtaaag caattgaaga
    88441 tgcgaagaaa aacttagttg aagtacctat ggttggttca acaatcccac acgaagtaat
    88501 cggtgctttt agtggtggta gaatcctaat gaaacctgct gtagaaggtt ctggggttgc
    88561 tgctggtgga ccagttcgtg ccgtattaga attagctggg gtagcagata tcacttctaa
    88621 atctttaggc tcaaatacac caatcaacgt tgttcgcgca acagttgaag gtttgaaaca
    88681 attgaaacgt gcagaagaag ttgcagaact tcgcggcaaa tcagttgaag aattaatcgg
    88741 ataaggagga caacaataat ggctgaatta aaagttactt taaaacgcag tattatcgga
    88801 cgtcctcaaa accaacgcga tacagttaaa gcgttaggtc ttacaaagat taacagttca
    88861 gtggttaagc cttctaatga agcaattaaa ggcatgatca atactgtttc tcacctagtg
    88921 gacgttgaag aaatttaatt tagaaaaatc ataactagaa cattaaggag gtgccaaacc
    88981 tatgaaactt catgaattaa aacctgctga aggttcacgc caagtacgta accgtgttgg
    89041 tcgtggtact tcatctggta acggaaaaac tgccggacgc ggacaaaaag gacaaaaagc
    89101 tcgttcaggc ggcggtgtgc gtttaggatt tgaaggggga caaacaccat tgttccgtcg
    89161 tttaccaaaa cgaggattca caaacgtgaa ccgtaaagac tatgcagtca ttaatcttga
    89221 tgtattgaac cgctttgaag acggagctga agtaactcct gttgctttag ttgaagctgg
    89281 aatcgtgaaa aacgaaaaag ctggaatcaa agtattagct aatggggaac taaacaagaa
    89341 attaactgtg aaagcagcaa aattctctaa agcagctcaa gaagcaattg aagcggctgg
    89401 tggatcaatc gaggtgatct aatgtttaga ctattaaaag atgcttttaa agtaaaagac
    89461 attagatcga aaatcctatt tactgtattt gtgttgtttg tatttcgcct aggtgcacat
    89521 attactgtac caggcgtaaa tgctgcttca ttgcaaaacc tttcttcttt gccatttttc
    89581 aacatgttga acttggtaag tggtagtgcg atgcaaaact tttccatttt ctcaatgggg
    89641 gtttcaccat acattactgc ttctatcgtc attcagcttc ttcaaatgga catcgtgcct
    89701 cgatttgttg aatggtcgaa acaaggggaa gttggtcgta aaaaactgaa tcaagcgact
    89761 cgatatctga cattagtatt ggcatttatc caatctgtcg gtatcactgc tggattcaat
    89821 caatacgctc aattaggttt cgtgaataat cctaacgtag caacttacgt aacgatcggt
    89881 ttgattctaa cagccggtac gatgtttgtt acttggctag gagaacagat cacagaaaaa
    89941 ggtatcggaa atggcgtatc aatgatcatt tttgcaggta tcatttctag attgcctgaa
    90001 agtatcagag aaatttatga agattacttt ataaatattg atccttcaga catatggaaa
    90061 tctgctattt ttgtggccat tttactagta gcggtcttgt tgatcgtgac attcgtaaca
    90121 tttttccaac aagctgaacg caagatcccg atccaatata cgaaacgcgt agctggcgcg
    90181 cctacaagca gctatctgcc gttgaaagta aatgcagctg gagttattcc agttatcttt
    90241 gctagttcgt ttattgctac gccaaatgct gttttacaag cattgagcgg tagattcggt
    90301 tctgaaaact ggtacaaagt gatgatggag atctttaact ataatacagc acctggtgct
    90361 acgatttata cgattctgat cgttgccttc actttcttct acgcctttgt tcaagtaaac
    90421 cctgagaaat tagcggaaaa cttgcaaaag caaggaagtt atataccaag cgtacgccct
    90481 ggtaagggga ctgaagagta tgtctcaaga ttactgatgc gtttaagtac agttggtgca
    90541 gtcttccttg gtttagtggc attgcttcca atcattgctc aaatggtttg ggatcttcca
    90601 caatcaatcg gattaggcgg tacaagctta ctgatcgtta tcggtgtagc tttagaaact
    90661 gcgaaacagt tagaaggctt aatgctgaaa cgtaaatatg ttggattcat tgagtaagaa
    90721 agcgtgttga ggtttcccct caacacacgt tctcacacat ttattaggag ggaaaacaat
    90781 gaacctcatt ttaatggggc tgccaggcgc aggtaaagga acacaagctg aaaaaatcat
    90841 tgatgcttat ggtattccgc acatctcaac aggagatatg ttccgcgcag cgatgcaaaa
    90901 cgaaacagct cttggcctgg aagcaaagtc ttatatggat aaaggtgcat tagttcctga
    90961 tgaagtaaca aatggaatcg tgaaagagcg actagctgaa ccagatacag agaaaggctt
    91021 cttattagat ggctttccac gtacgctaga tcaagcaaaa gctctggacg caatgctaaa
    91081 agatttgaat aaaaaaattg atgctgtcat cgatatccat gttggtgaag aaatcttggt
    91141 cgaacgtcta gccggccgat ttatctgcag caattgtgga gcaacttacc ataaaatttt
    91201 caaccctaca aaagttgaag acacatgtga tcgttgcggt gggcatgaat tctatcaaag
    91261 agaagatgat aaacctgaga cggttaaaaa tcgtctggct atcaacatca aaaatagtga
    91321 accaatcttg gcttattata aagaacaagg tttgctaaac actattgatg gtgctcgtga
    91381 aatcgatgct gtatttgcag atgtacaaaa aatcattgaa aaatagcacg attgcccgta
    91441 gccgacttgc attatgcagt tactcttgtc aagttcaata aattatgata gaatatgtca
    91501 gtccgacttc tagagaatcc tctagatgat ccattttgag aattttgagg tgggaactgc
    91561 agtttccgcc attttatcgt gtgaaaatgc gaaacaagta caaggaggta ctgtgcgtgg
    91621 caaaagaaga tatgattgaa gtcgaaggta cagtcgtcga aactttgccg aatgcaatgt
    91681 ttaaagttga actagaaaac ggacaccaag ttcttgctac tgtttcaggt aaaattcgta
    91741 tgcattacat ccgtatctta cctggtgaca aagtaacggt tgaattgtct ccatacgatc
    91801 ttaaccgtgg ccgtattact tatcgcttta aataattgta ctccgtaaaa ctacaggagg
    91861 tattatcatg aaagtaagac catcagtaaa accaatgtgc gaacattgta aagtaattcg
    91921 tcgtaaagga cgcgttatgg tgatttgccc agcaaatcca aaacataaac aacgtcaagg
    91981 ataattggag gtgctataaa tatggctcgt attgcaggag tagatattcc tcgtgataaa
    92041 cgtgtagtag tttcacttac ttatatctat ggtattggta acactactgc taaaaagatc
    92101 ttagctgatg ctggtgtatc tgaagatgtt cgcgttcgtg aattaacgaa tgaacaaaca
    92161 gatgctatcc gtgcagaagt agataaatta aaagttgaag gggatcttcg tcgtgaagtg
    92221 aacttgaaca tcaaacgttt gatggaaatc ggttcttacc gtggaatccg tcatcgccgt
    92281 ggattgcctg ttcgtggaca aaacacgaaa aacaatgcac gtactcgtaa aggcccagct
    92341 cgcgctatcg ctggcaagaa aaaataattc ttaagtgaag gaggttaaaa cttcatggca
    92401 gcaaaaaaag tgaatcgtaa acgccgtgtg aagaagaata tagaagcagg tattgcacat
    92461 atccattcta cattcaataa cactatcatc atgatcactg atactcacgg aaatgcatta
    92521 gcgtggtcat cagctggttc attaggtttc aaaggaagca aaaaatcaac tccttttgcc
    92581 gctcaaatgg cagcagaaac tgctacaaaa gcagcaatgg aacacggatt aaagactgtt
    92641 gaagtaacag ttaaaggacc tggttctgga cgtgaagcag caattcgttc attacaagct
    92701 acaggtttag aagtgactgc aattcgtgac gtgactccag ttcctcataa tggtgcccgc
    92761 cctccaaaac gccgtcgtgt ttaatgagtg ccgctcattt tgagttttat cagtaacgtc
    92821 attgaacaag acacttttcg ttttgaaagg ggtaaagata agaatgattg agttcgaaaa
    92881 accaagaatc gcaaaaattg atgaagaaaa agattatggc aagttcatcg ttgaacctct
    92941 tgaaagaggg tatgggacta ctttagggaa ttccctacgt cgtattttat tatcttcttt
    93001 gccaggtgct gccatcacta atattcaaat cgatggtgta ctgcatgaat tctctaccat
    93061 caaaggtgtt agagaagacg tcactcaaat catcttgaac attaaaggct tagcgctgaa
    93121 aatgtatggc caagaagaaa aaacccttga aatcgatatt accggacctg ctacagtaac
    93181 tgctggcgat attatcgttg atagtgatgt agaaattctt aacaaagata tgtacatctg
    93241 tagtgtctct gaaggagcta cgttccatgc tcgcttaaca gtgaaacctg gacgtggtta
    93301 tgttcaagca gatgaaaata aaaaagaaga tatgccaatt ggcgttcttc cagtcgattc
    93361 aatctacaca cctgttcgtc gtgtgaacta tcaagttgag aacacacgtg ttggacaccg
    93421 tgaagatttt gataaattga cgatggagat ttggacagat ggttcaattg aaccgttgga
    93481 agcaatgagt ttgtctgcga aaatcatgac tgaacatttg gatatttttg tgaacttaac
    93541 tgatgaagca aaaaatgctg aaatcatggt cgaaaaagaa gaaacacaaa aagaaaaaat
    93601 gttggaaatg acgatcgaag agttagactt gtctgttcgc tcttacaatt gtttgaaacg
    93661 tgcaggaatc aatactgtac aagaacttac aaataaatct gagcctgaaa tgatcaaagt
    93721 ccgtaactta ggacgtaaat cattagaaga agttaaagca aaattgcacg acttaggttt
    93781 aggtttacgt aaagaagatt aaaacctttt acgaaggagg gaaaccaacg tgagttatcg
    93841 taaactagga cgcacatcta gccaacgtaa agcgatgttg cgtgatttaa caactgattt
    93901 attaatcaac gaacgtattg tgactactga agctcgtgcg aaagaagttc gctcaactgc
    93961 agaaaaaatg atcactttag gtaaacgcgg agatttgcat gcacgtcgtc aagctgcggc
    94021 tttcgtacgt aatgaagttg ccagcgtgcg tgaagaaaat gaggacatcg tcattgaatc
    94081 agctttacaa aaactgttca acgatattgc acctcgttac gcagaacgtc aaggcggata
    94141 cacacgcatt ttgaagacag aaccaagacg cggtgatggt gcaccaatgg ttgtcttaga
    94201 acttgtctaa aatcacacta ataacatcac tttatagatg attcttttag agtgttatga
    94261 tgttggaaga tatttttccg agtctagctc agcgctgctc ctcaagcgaa tatcgcttgg
    94321 ggagtaagca ctcatcataa agtgtttttt tataccttca attacctgtc atgacagata
    94381 tgtttgaagg cataaaaaaa cattttaagc gagaaataat tgaaaatttt gtcttttttt
    94441 gatagtgtta gtaggaactt taggatgaag aaggagtaat catggagcca attattgaat
    94501 tagaaaagat aaattataaa taccagccag atgatcttcg ccccgcattg aaagatgtct
    94561 cctttacaat tgataaaggt gagtggatcg ctattattgg acacaatggt tcaggaaaat
    94621 cgacacttgc caaaacgatc aatggattgc ttctgccaga atcagggatc gtgaaagtgg
    94681 gaaatcaaat attggatgaa gaaaatattt ggacgatccg acagatggtt ggaatggttt
    94741 ttcaaaatcc agacaatcag tttgttggtt cgacagtaga agatgacgtt gcttttggtc
    94801 ttgaaaatca agggattccc agagaagaaa tgctagtgcg tgtgaaagat gcacttgaga
    94861 aagttagaat ggctgagttt gcttcacgcg agcctgctcg tttatcagga gggcaaaaac
    94921 aacgtgttgc cattgccggc gtggttgcct tacgacctga tatcattatt ttagatgaag
    94981 caaccagtat gctagatcca gaaggtcgtg aagaagtgat ctctacgatc aagaagatca
    95041 aagaagaaag tcaattgacc gttatatcta tcactcatga tattgatgaa gcagctaatg
    95101 ccaatcggat actagtgatg agacaaggcg aacttgtccg tgaaggtaca ccaaaagaaa
    95161 ttttttctgc tggtcctgaa ctgatcgact taggtctaga tctaccattt cctgaaaaac
    95221 tgaagagcgc actgaaagaa cgtggggttg atgtgcccag tgaatatatg acagaagaaa
    95281 ggatggtgga ttggttatgg acatccgttt tgaacaagta gacttcactt atcagccgaa
    95341 tacaccattt gaacaaagag ccttatttga catcaatatg acgatcaaag aaaacagtta
    95401 tactgcatta gtagggcata ccggaagcgg gaaatcaacc ttacttcagc atttgaatgc
    95461 gcttgttaag ccaacaagcg gtacagtcca tattggagaa cgagatattc agccggatac
    95521 ggataataag aatttaaagc ccatccgaaa aaaagttggc atcgtctttc agtttccaga
    95581 agcacagcta ttcgaagaaa cggtagcaaa agacattgct ttcggtccta aaaactttgg
    95641 tgtcagcgaa gaagaagcac tagtcctagc aaaagaaaca ttagaacaag ttgggctgga
    95701 tgaaagctat ttggaacgtt cgccatttga actttcagga gggcaaatgc gtagagtagc
    95761 aatcgctggt gtgcttgcca tgaggccgga agtacttgta ttggatgaac caacggcagg
    95821 acttgatccc cagggtagaa aagaaatgat ggagatgttt tggcgtcttc ataaggaaca
    95881 gcagatcact attgtattag ttacgcattt aatggatgat gtagccaatt ttgcagatta
    95941 tgtctatgtg ttggaaaagg gacgaatcgt taatagcggg gagccgcaag aggtctttca
    96001 aaacattgaa tggctaaaag aaaaacaact aggcgtacct actgccaccg agtttgcaga
    96061 agaattaatg gctaaaggga tgaactttgc taccttgcct ttgacagctg aagaattagc
    96121 agacgctatc gtccattatg ctggaggaaa tgcccatgat gaataaattg atatttggtc
    96181 gttatatgcc tggagattcc ttgatccatc gattggatcc ccgagctaaa ttattagcca
    96241 gtttttattt tatcggaatc atttttttag caaataattg ggaaacgtac cttttgttgg
    96301 catttttcac attactttct gtcttcttgt caaaagtaga tttgaaattt tttatccgag
    96361 gcattcgccc attgatctgg ctaatcctct tcactgtagc tttgcaggtc ttgtttacaa
    96421 gtggcggaga aacttattgg agttggggtg ttttccatat cactgaattt ggtgtgatga
    96481 acggcctctt tattttctgt cgattcgtgt tgatcatttt tatgtcgact ctactcacgc
    96541 ttactacacc gcctttggaa ttatcggatg cgattgagta cttgcttcgt ccattgaaag
    96601 ccgtacggtt cccagtccac gaaatttcat tgatgctgtc gattgctctt cgttttgtgc
    96661 ctacattgat ggacgaaaca gaaaagatca tgaacgctca gcgcgctcgc ggtgttgatt
    96721 ttggtgaagg aaacctagtt cagaaaatga aagcagtcat tcctttgctg attcctttat
    96781 ttgtcagcag cttcaaccgt gcggaagatc ttgcgactgc tatggaagca agaggctaca
    96841 aaggtgggga aggaagaacg aaatatcgtg tattgcattg gcataataga gacacgatgg
    96901 ttatcattgc ttttgtatta ttaactgtgc tactgatctt tttgagagga taaacgttca
    96961 gtgggccgct atctattctt ttcttgctga tctgtttgca agaaaggatg cgagcagtgt
    97021 atgacaagaa atattagggt ctgtggcaga ctaaggtcac aggcctcttt cttttatctg
    97081 aaaagaaaga acgaaaaaat tttataaaaa aatcattatt ccagataacc ttttaggaac
    97141 aaaggagaga aaaacatggt acgttacaaa gcaatcattg catacgacgg aacaaatttt
    97201 aatggattcc aaaaacagcc aaatgggcgt actgttcaag aagaagtaga aaaaacatta
    97261 caaaaaatgg caaatggaaa agaaatcacg gtctttggtt cgggaaggac agatgccgga
    97321 gtacatgcga ttgggcaagt aatccatttt gattatccag aggagcgtcc gttagaacga
    97381 atgcgtttcg cgctagatac ccagtcacca gaggatattg ctgtaagaca agtagaaatt
    97441 gtttcagaag acttccacgc aagatatttg gtcaaagaaa agacgtatca atttcgagtg
    97501 gatataggga aaccacgcag tccttttcga cgccattatg caagttattt tccttaccct
    97561 cttgatttgg aaaaaattca gcgggcgctt cctgatttat tagggactca cgatttcacc
    97621 tctttttgtg catcaggaag ctcgatagag gataaagtac gtacgatcta cgaggctaag
    97681 atggaggtaa atgaaacaaa agacgaattg ttgtttacat ttaggggaaa tggcttcttg
    97741 tataagatga tacggatctt agtaggaacg cttttgaaaa ttggcaatgg ccgattgcct
    97801 gaagatagta tcccagatat tattgcgaaa aaagatcgta atgcagcagg acctacagca
    97861 catcctgaag gactttattt gtatgaagtg aaatatgaat aagaggttgt gacagaagtg
    97921 gtcagcttca agaaataaga aggaatttac gaaaattact tctcaaaatt ttgcaaaatt
    97981 tcggcttatt ttcgaaggag ctacttctgg aacaccgtga atcagaggtt gtgaaaaagc
    98041 tgccctgcat caagtaataa gaacaaccaa acaaaaatag ctcgtcatat ttttcgtttg
    98101 gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt ttaaggaaga ggttgtgaaa
    98161 tcagcgtttt gacctgagta ttagagaaac aaacggaaaa atagttcctc atattttgcc
    98221 gtttgttgag ctaataccga aggtcagctg attgaacacc gtttaagaaa gaggtcgtga
    98281 gcctgtcatt taactctgag tcacaagtaa caatttcaaa aaacagcttc tcatctttga
    98341 ggaatatcgt gacttgtgga cgaggcgttg ataagtgaat catctttttg ttacctattt
    98401 tttctgtaac caaaaaacga tggaacgttg ttttgattgc ccttaatgag gacagtcgga
    98461 acaggtttgt catttttat
//
LOCUS       DACKAC010000003        86975 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.003, whole genome shotgun sequence.
ACCESSION   DACKAC010000003 DACKAC010000000
VERSION     DACKAC010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 86975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 86975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..86975
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.003"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(606..962)
                     /locus_tag="I0B46_000196"
     CDS             complement(606..962)
                     /locus_tag="I0B46_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAR2082530.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(976..1386)
                     /locus_tag="I0B46_000197"
     CDS             complement(976..1386)
                     /locus_tag="I0B46_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAR2082531.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            complement(1732..3918)
                     /locus_tag="I0B46_000198"
     CDS             complement(1732..3918)
                     /locus_tag="I0B46_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAR2082532.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNISVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            4136..4819
                     /locus_tag="I0B46_000199"
     CDS             4136..4819
                     /locus_tag="I0B46_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAR2082533.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            complement(4900..5619)
                     /locus_tag="I0B46_000200"
     CDS             complement(4900..5619)
                     /locus_tag="I0B46_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAR2082534.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(5931..6137)
                     /locus_tag="I0B46_000201"
     CDS             complement(5931..6137)
                     /locus_tag="I0B46_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2082535.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            6382..6984
                     /locus_tag="I0B46_000202"
     CDS             6382..6984
                     /locus_tag="I0B46_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAR2082536.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            7125..8406
                     /locus_tag="I0B46_000203"
                     /pseudo
     CDS             7125..8406
                     /locus_tag="I0B46_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
     gene            8419..9147
                     /locus_tag="I0B46_000204"
     CDS             8419..9147
                     /locus_tag="I0B46_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAR2082537.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(9216..10739)
                     /locus_tag="I0B46_000205"
     CDS             complement(9216..10739)
                     /locus_tag="I0B46_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAR2082538.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            10991..12172
                     /locus_tag="I0B46_000206"
     CDS             10991..12172
                     /locus_tag="I0B46_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAR2082539.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(12232..13860)
                     /gene="treC"
                     /locus_tag="I0B46_000207"
     CDS             complement(12232..13860)
                     /gene="treC"
                     /locus_tag="I0B46_000207"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAR2082540.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(14311..17022)
                     /locus_tag="I0B46_000208"
     CDS             complement(14311..17022)
                     /locus_tag="I0B46_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAR2082541.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGTIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(17032..17733)
                     /locus_tag="I0B46_000209"
     CDS             complement(17032..17733)
                     /locus_tag="I0B46_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAR2082542.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            17919..18518
                     /locus_tag="I0B46_000210"
     CDS             17919..18518
                     /locus_tag="I0B46_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAR2082543.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            18599..19612
                     /locus_tag="I0B46_000211"
     CDS             18599..19612
                     /locus_tag="I0B46_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAR2082544.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            19720..20637
                     /gene="rbsK"
                     /locus_tag="I0B46_000212"
     CDS             19720..20637
                     /gene="rbsK"
                     /locus_tag="I0B46_000212"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAR2082545.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            20665..21060
                     /gene="rbsD"
                     /locus_tag="I0B46_000213"
     CDS             20665..21060
                     /gene="rbsD"
                     /locus_tag="I0B46_000213"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAR2082546.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            21060..22433
                     /locus_tag="I0B46_000214"
     CDS             21060..22433
                     /locus_tag="I0B46_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAR2082547.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            22790..24934
                     /locus_tag="I0B46_000215"
     CDS             22790..24934
                     /locus_tag="I0B46_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAR2082548.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(24986..26027)
                     /locus_tag="I0B46_000216"
                     /pseudo
     CDS             complement(24986..26027)
                     /locus_tag="I0B46_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
     gene            complement(26304..28106)
                     /locus_tag="I0B46_000217"
     CDS             complement(26304..28106)
                     /locus_tag="I0B46_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAR2082549.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(28120..28872)
                     /locus_tag="I0B46_000218"
     CDS             complement(28120..28872)
                     /locus_tag="I0B46_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAR2082550.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(28881..29243)
                     /locus_tag="I0B46_000219"
     CDS             complement(28881..29243)
                     /locus_tag="I0B46_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAR2082551.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(29419..30291)
                     /locus_tag="I0B46_000220"
     CDS             complement(29419..30291)
                     /locus_tag="I0B46_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAR2082552.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(30342..30830)
                     /locus_tag="I0B46_000221"
     CDS             complement(30342..30830)
                     /locus_tag="I0B46_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAR2082553.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            complement(30968..31759)
                     /locus_tag="I0B46_000222"
     CDS             complement(30968..31759)
                     /locus_tag="I0B46_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAR2082554.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GKSYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(31814..32422)
                     /locus_tag="I0B46_000223"
     CDS             complement(31814..32422)
                     /locus_tag="I0B46_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAR2082555.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(32415..32732)
                     /locus_tag="I0B46_000224"
     CDS             complement(32415..32732)
                     /locus_tag="I0B46_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAR2082556.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(32888..33616)
                     /locus_tag="I0B46_000225"
     CDS             complement(32888..33616)
                     /locus_tag="I0B46_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAR2082557.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            33775..34656
                     /locus_tag="I0B46_000226"
     CDS             33775..34656
                     /locus_tag="I0B46_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAR2082558.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERANRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(34893..35936)
                     /locus_tag="I0B46_000227"
     CDS             complement(34893..35936)
                     /locus_tag="I0B46_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAR2082559.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(35939..36607)
                     /locus_tag="I0B46_000228"
     CDS             complement(35939..36607)
                     /locus_tag="I0B46_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAR2082560.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            36749..39085
                     /locus_tag="I0B46_000229"
     CDS             36749..39085
                     /locus_tag="I0B46_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAR2082561.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSD
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(39200..39511)
                     /locus_tag="I0B46_000230"
     CDS             complement(39200..39511)
                     /locus_tag="I0B46_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2082562.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            39692..41167
                     /locus_tag="I0B46_000231"
     CDS             39692..41167
                     /locus_tag="I0B46_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAR2082563.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPTMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            41214..41798
                     /locus_tag="I0B46_000232"
     CDS             41214..41798
                     /locus_tag="I0B46_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAR2082564.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            41800..42219
                     /locus_tag="I0B46_000233"
     CDS             41800..42219
                     /locus_tag="I0B46_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAR2082565.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(42500..42784)
                     /locus_tag="I0B46_000234"
     CDS             complement(42500..42784)
                     /locus_tag="I0B46_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAR2082566.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(43016..43726)
                     /locus_tag="I0B46_000235"
     CDS             complement(43016..43726)
                     /locus_tag="I0B46_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAR2082567.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(43723..45195)
                     /locus_tag="I0B46_000236"
     CDS             complement(43723..45195)
                     /locus_tag="I0B46_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAR2082568.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            45333..45806
                     /locus_tag="I0B46_000237"
     CDS             45333..45806
                     /locus_tag="I0B46_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAR2082569.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(45959..46510)
                     /locus_tag="I0B46_000238"
     CDS             complement(45959..46510)
                     /locus_tag="I0B46_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAR2082570.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            46615..47145
                     /locus_tag="I0B46_000239"
     CDS             46615..47145
                     /locus_tag="I0B46_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAR2082571.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(47628..48071)
                     /locus_tag="I0B46_000240"
     CDS             complement(47628..48071)
                     /locus_tag="I0B46_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAR2082572.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(48087..49247)
                     /locus_tag="I0B46_000241"
     CDS             complement(48087..49247)
                     /locus_tag="I0B46_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAR2082573.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            49754..51454
                     /locus_tag="I0B46_000242"
     CDS             49754..51454
                     /locus_tag="I0B46_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2082574.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(51556..52389)
                     /locus_tag="I0B46_000243"
     CDS             complement(51556..52389)
                     /locus_tag="I0B46_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAR2082575.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(52693..54858)
                     /locus_tag="I0B46_000244"
     CDS             complement(52693..54858)
                     /locus_tag="I0B46_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAR2082576.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            55118..57412
                     /locus_tag="I0B46_000245"
     CDS             55118..57412
                     /locus_tag="I0B46_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAR2082577.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            57405..58070
                     /gene="pgmB"
                     /locus_tag="I0B46_000246"
     CDS             57405..58070
                     /gene="pgmB"
                     /locus_tag="I0B46_000246"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAR2082578.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            58063..59070
                     /locus_tag="I0B46_000247"
     CDS             58063..59070
                     /locus_tag="I0B46_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAR2082579.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            59092..60111
                     /locus_tag="I0B46_000248"
     CDS             59092..60111
                     /locus_tag="I0B46_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAR2082580.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(60392..61759)
                     /gene="nhaC"
                     /locus_tag="I0B46_000249"
     CDS             complement(60392..61759)
                     /gene="nhaC"
                     /locus_tag="I0B46_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAR2082581.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(61868..63289)
                     /locus_tag="I0B46_000250"
     CDS             complement(61868..63289)
                     /locus_tag="I0B46_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAR2082582.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(63532..65409)
                     /gene="tdc"
                     /locus_tag="I0B46_000251"
     CDS             complement(63532..65409)
                     /gene="tdc"
                     /locus_tag="I0B46_000251"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAR2082583.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(65700..66956)
                     /locus_tag="I0B46_000252"
     CDS             complement(65700..66956)
                     /locus_tag="I0B46_000252"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAR2082584.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            67536..68837
                     /locus_tag="I0B46_000253"
     CDS             67536..68837
                     /locus_tag="I0B46_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAR2082585.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(68930..70318)
                     /gene="ltrA"
                     /locus_tag="I0B46_000254"
     CDS             complement(68930..70318)
                     /gene="ltrA"
                     /locus_tag="I0B46_000254"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAR2082586.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
     gene            complement(70805..71404)
                     /locus_tag="I0B46_000255"
     CDS             complement(70805..71404)
                     /locus_tag="I0B46_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAR2082587.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            71658..72539
                     /locus_tag="I0B46_000256"
     CDS             71658..72539
                     /locus_tag="I0B46_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAR2082588.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            72552..74162
                     /locus_tag="I0B46_000257"
                     /pseudo
     CDS             72552..74162
                     /locus_tag="I0B46_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
     gene            complement(74239..74898)
                     /locus_tag="I0B46_000258"
     CDS             complement(74239..74898)
                     /locus_tag="I0B46_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAR2082589.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(75181..75846)
                     /locus_tag="I0B46_000259"
     CDS             complement(75181..75846)
                     /locus_tag="I0B46_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2082590.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(75993..76598)
                     /gene="dhaL"
                     /locus_tag="I0B46_000260"
     CDS             complement(75993..76598)
                     /gene="dhaL"
                     /locus_tag="I0B46_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAR2082591.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(76626..77609)
                     /gene="dhaK"
                     /locus_tag="I0B46_000261"
     CDS             complement(76626..77609)
                     /gene="dhaK"
                     /locus_tag="I0B46_000261"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAR2082592.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(77611..78006)
                     /gene="dhaM"
                     /locus_tag="I0B46_000262"
     CDS             complement(77611..78006)
                     /gene="dhaM"
                     /locus_tag="I0B46_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAR2082593.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            78160..78480
                     /locus_tag="I0B46_000263"
     CDS             78160..78480
                     /locus_tag="I0B46_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAR2082594.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(78592..79461)
                     /locus_tag="I0B46_000264"
     CDS             complement(78592..79461)
                     /locus_tag="I0B46_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAR2082595.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(79557..80861)
                     /locus_tag="I0B46_000265"
     CDS             complement(79557..80861)
                     /locus_tag="I0B46_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAR2082596.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFI
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEEISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(80858..81367)
                     /locus_tag="I0B46_000266"
     CDS             complement(80858..81367)
                     /locus_tag="I0B46_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2082597.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(81542..83677)
                     /locus_tag="I0B46_000267"
     CDS             complement(81542..83677)
                     /locus_tag="I0B46_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAR2082598.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(83760..85415)
                     /locus_tag="I0B46_000268"
     CDS             complement(83760..85415)
                     /locus_tag="I0B46_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAR2082599.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(85417..85731)
                     /locus_tag="I0B46_000269"
     CDS             complement(85417..85731)
                     /locus_tag="I0B46_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAR2082600.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            85901..86161
                     /locus_tag="I0B46_000270"
     CDS             85901..86161
                     /locus_tag="I0B46_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAR2082601.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            86175..86489
                     /locus_tag="I0B46_000271"
     CDS             86175..86489
                     /locus_tag="I0B46_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAR2082602.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
BASE COUNT        25589 a        17712 c        15507 g        28167 t
ORIGIN      
        1 tgtttgttgt cttgaattga atcaagacgc cctacacatt tggtttgtct tactttgttc
       61 agttttcaaa ggtctacttc atcaggagtg ttccccgatt cgtttttggc tgccgtagca
      121 acttttatat catatcagag agacaagtaa ttgtcaactc ttttttaaaa gtttttttga
      181 tttcgttttt cgaaatctta cagcaactca tttatcataa cttatcattt gttatttgtc
      241 aagaactttt ttgaagtttt tttcgataga atcaagcttc acaagtggtt cttttttaac
      301 aactcagtta tcttatcatg tcatttctaa catgtcaaca actattttat atgtttttta
      361 aaatactatt ttattgcttt gctacttaaa acaacagaaa tgaatatatc atgtaaattt
      421 acacatgtca atatattttt aactctatat ccccacctag atttgaattt tgaccacata
      481 aattgagcat gtctctatag tagaaaaata aagtcaaaaa aagaacacat aacacgcttg
      541 atgctaagtg ttcgactgtt acaactgtat gaaatgctca gtgacttgat taaaacagtt
      601 aaagtctatt tatttaagat aagctgttcc atcaactgct cattgtatcc aataaataga
      661 acttcatcga taataatcaa aggacgtctg aggagattgg tatgttgtgt gcataaaaaa
      721 atcagttgtt tttttcctac acaggaaagg tcaatcgcaa tgttgtgaaa agcctcagac
      781 caagtcgcaa atagctgttc tgcttgcact tcttcaaaac tgcataattc ttctgcttct
      841 tctgtactta gatcgcgttc ttctaagtca acaaattgat aaggcaattg atggtgttct
      901 aaccattgtt tcgccttcag acttgttaca cagtgcgctg atccatagac tttcacttcc
      961 acgaaggctc cctccctatg aatttaagat gttattgttt tgtaaataga tcagatttct
     1021 ggcaacacat cgaatttcac gagggacaaa tgaccggatc tcatcttcat taaatccgat
     1081 tagtattctt ttttcatcaa tcaaaatagg tcgtctcaat aatgaaggat attctttgaa
     1141 taagacgatc atttgcttta aagttaattc gttaaaatct atgcccagct tttcgtaaat
     1201 tttcgaccga gtggaaataa tatcttgaac tcctccttca gtcgctgcca agatcttttt
     1261 gatctcactt tctgttaacg gttcggcaaa aatattcttc tcttcaaatt caagttgatt
     1321 cgcttgcaac catgctcttg cttttctaca ggaagtacag ctaggcgaag tatatacttt
     1381 tatcattttc tcactccttt ttaattataa tccaattcta cagtaatttc ttttaaaata
     1441 ccaattatta gctaataaaa tataaaaaaa atcaaaataa ctgattattt acgtttgaat
     1501 aatgagaatt tttctatata ttctctattt tgataatagt attctcattt tcaaaataaa
     1561 taagggaggt gtgacaaaat tcttgtcaca cctccctttt ccgaataaac tgtgttccag
     1621 aaatagcttc ttcgaaaata agctgaaatt tcacaaaaat ttgagaagca attttcgtac
     1681 attccttctt ctttcttgaa actgaccact tctgtcacac cctctcttct atcactttcc
     1741 ttttttcaca cgactcccgg tttctttgat aaatctcgat actttcaatt ttcttgaatt
     1801 tttcatttga ggtacacata gatacagacg atttcttgct ctagtcatcg caacataaaa
     1861 tagtcttcgt tcttcttcta tcgtcgttac tggttctgct tttatttcat taaagtcgat
     1921 tactttcttt tctgtcgctc cgttataagg caaacttcct tcgttacaac caatcaaaaa
     1981 gacatttcgg taactaagac ctttactttt gtgaatgctc tggatacata gtgcatcaag
     2041 ttttccttgt ctgtaagcaa tcaagcgggt ttttaattct ttagctgtcg cttgggtttc
     2101 ttgcgataag gcttggagtc ttctgaaagt agcatgctgc tttacttctt ctcgtatcca
     2161 ttcttgatca ctctggtcga tttccttagc tatacgtggc agatgaagat agctttgaac
     2221 aagcgaatag atttcttgat gctggtctgt tttacgcaac gcttcctcaa actctcggat
     2281 ttctgcagat ttcgatttta aattggggct tatcgacaaa atataatctg caacggatat
     2341 attttgatat ttaggagaaa agtattgatc gcattcactt tttttcaggt agcagtctgc
     2401 catcagctgc cggagcaatt gctggtaggc ctgatttttc ttttgcagat tcagtgctgt
     2461 ttgccaatga acaatttttt gatagaaatg ttggaaagac ttgataccaa agataccttc
     2521 atatggacta tctgcttcca gtacaaaagg aatattcgtt ttcagcagag attggacaag
     2581 ctgctgcgtt tgtgaagctg atcggtataa tatgattgat tccttatatt tcccattttt
     2641 ttgaataaat gtctgaattt gtttgattag ccactgactt tctgctttag tatcagaaaa
     2701 caaataggct tccataacac ctgcttcttt ttgtgctgga atcatcgcct ttggtatccg
     2761 atcattttta ttatagctga tcaatctatt tgctccttgg acaatagctg cccgagaacg
     2821 ataattagtt aataagaaaa gagttttggt aggatattct ttttcaaagt tcttgatgta
     2881 tgctggctct gatcctctga atccatagat actctgatca tcatctccaa caacaatcaa
     2941 cttgatcctg ttttcctttg tcatccgttt cacgatttcc cattgaagtg gattagtatc
     3001 ttgaaattca tcaatgaata gataatcata tttttggccg atataatgct ggatctcctc
     3061 attctccaaa gcgcttttca gattttggag tacatcattg aactctagca attgctggct
     3121 tcttttttgt tcttggagtt ccgtgagcaa tccatacagt cgttctgata tcggctgtcg
     3181 tgcattctta tcaatggaat catatttttc tttaactaac tgaaccattt cttctactga
     3241 aaggttcata ttctgccaat agtctaaccg agaagcaatc gttttttctc catattgatc
     3301 aaatatataa agattatatt tacgtatcag ctgttgcaaa acagcattca ctttttgtgc
     3361 agtatccttt gaaaagtctc cttgtattcc ttgcattgct acttcaggaa attttcgaca
     3421 aatttcattg tatatcctaa aaaagatact atggaatgtt ccgatatgga cagcggtttt
     3481 tctctggctg aaattatcta ctcgtttttt tagttcattt gcagcgttcc ttgtaaaagt
     3541 gatcattagt atgcgttctt cgtctacttg tttttgcgtc atcagataaa cagcttttgc
     3601 acagattgtt tgcgttttgc cgctcccagc tcctgctatg attcgaagat gtttcttcat
     3661 atcatatgtg atagctgctt tttgttcttg attgaagcga ttataggaac gtttctccat
     3721 cattttaaag aaagagctgt ttccttcact ttcttttact tgtacttctt cctcaataag
     3781 tccgggtgct tcgccataag ccgcttcata ggtagcaatg atttcttctt gtagccgaaa
     3841 gacttgttga agctttttat acttgttctc ccagcgtttc aattcttttt gtaatcggag
     3901 aatttctttt tcattcaact gattgatttc cttcccatct ttcttctttg aatttgttta
     3961 tttcaagttg gttatttaaa gtttcgttgt ctattttttc tttatactaa cagacctttc
     4021 taaaaatgaa aatgggaagc gatatgattt tagttttctg aaaatttttc ctttttttca
     4081 atcgtttatg ctaagataag gaagacaaaa atattcataa aaggtgatga aaaaaatgca
     4141 gacttccttt cacgaggggc gtacaatagg acttttgctg acatttatcg gaggagccat
     4201 ggacagttat acttatatcc aatatggtgc ttttgcctct gctcaaactg gcaatatcat
     4261 tctatcgatc atccaagcat ttgacggaga atggctaagt gttggtaaaa aagtattgtc
     4321 tacccttttc tttttcttag ggattcttct aacaaagtat ctaattgatt attttagaaa
     4381 aaaagaaaaa catttctggc gcttgtttgt tctatattat gaagcagtga tttttttctt
     4441 tgttagtttg acacccgtcc atgagcatcc cgcattagta acgattttga ttgcttttac
     4501 tgcatcgatc cagtgggtag cttttgataa aatcaacggg cgtgcctaca ctaatttatt
     4561 tacaactgga aatttaaaag gggtagcaac gaacttatat gattattgta tttctaaaga
     4621 aaaagcagat tttgatcgat ttttccacta tctaagtgtc gttcttgcct ttatcgcagg
     4681 agctattgtc cttgttttct gtcatcatct gtttggcgcc cattcgattt tgatcgtttc
     4741 cattttattt ttgatcatgg ccctcactca gacatttcat ttatggcaat tttatcatac
     4801 gaatcggttc actatttgat ctcaatgaat caaaaaaact aagcaagatg ctgctcacag
     4861 agaaatatct gcgagtgagc aatttgctta gtttttatat tatttagttg taaagattcc
     4921 ctttgctttc gggttatttg tagtgtagcg gatcgactgg gcatctgcct cttctgtcat
     4981 tttctttgcc cgcatcttga tttcttgtac gatttgttct ccacatgccg gatcggctac
     5041 gaaatcatat ttgtccccat cgatcactat tttgggacat atatcaaact gttcatacca
     5101 ctcttcgtat cgtcgattga gttctttata gtaggaatag agttcaggat cttgttctag
     5161 ctgttcatat tcccggcctc tttttttgat tctcgctaac atagtttcaa aggatacacg
     5221 aatataaacc agtagatctg gtttcttttg tggcgaaatt tcttctaact ccttcagcat
     5281 cgtttctaaa agaccttcat actgctttac ttctacttca gaaacacgcc ctaaatctgc
     5341 attcaaatga aacagcatac tatcttcgta gatggagcgg tctagtacgt tgtcatcatg
     5401 gctgaaagca tctttgattg ccaaaaagcg tttgttcaaa aagaaaatct gtaataaaaa
     5461 agtatacttt tctggattag aataaaatag cggcaatact tcattatctt ccacgttctc
     5521 ataaaaaggt tttgtttcta aatcttgggc aagcatttca gttagtgtgc ttttccctgc
     5581 accgatcgtt cctgcaacaa tgatcacaat tatcggcatc cttcctgtaa taaaatctgt
     5641 tcttcatttt actacttcat ttatagaaat aaaagccttg actttatctg ctccttaaaa
     5701 gctacttctc taaaagtagc caaaagtaac tcctattcat gaaaataaag gctgtgacaa
     5761 aagtcttgtc gcagcccttt tttcaaataa acagtattct cctgccaaca cctcggtcac
     5821 aaggcaatat tcataaaaca tgaaaagctg ttttttaaca ttattccttg taactcagag
     5881 ctaaacggga gactcacaac ctctttatgc tctcaacatt ttatctgttt ttattcttct
     5941 attgtacttg atttgcgtac aactaactgc cagtgcagat tatgagcggt atcattgtct
     6001 tctactgcgc atttcatgac acgtatcatc acactatttt catagatttt ttctattttt
     6061 ccttttattg gtgattcaaa taaatcagat ttaaatgcat atgtttcgcc gacttcaaac
     6121 aattgttcat tttccataag aatccctcca tcacctttta taaggtcagt aattttttaa
     6181 cataaatcac tcgaaaaagc acaaacttgt cataaaaagt acttttcgta tcaaaactgt
     6241 cacttttttt ggtaaggttc ttggaaaacg aagatttttt taggagatca tgacggtttt
     6301 gatgtcaaaa ccacttttta tgaaacaaaa gacgtttttt atttttttta gcataaaatt
     6361 tgtggtggag gtacgtatga gatgaaagat aaagttgtga acaaattatt agaagcctta
     6421 ttcagcggag acaaatcgga agaagatatg gtgtatgtcc aagtaaaatt tgtcttagaa
     6481 gttttattga ataatcttgg aaaactagcg gtcgttctag tcttttctct ggtgacaggc
     6541 tcatgggcgg aaacaggaat cacctttctt tcttatatct gcatccgcag atatgcttat
     6601 ggattgcatt ccgactctga attcgtttgt ctgctgtgga cactacttta tttatggggc
     6661 gttcccttgg tgatgaaaca tctgcaattg actatctcct tcccaatgat ggtctttttg
     6721 ttaatcagct gctttttgtt actgcttcgc tatggatcaa gaggtacagc aatcaatccc
     6781 attgaacccg agaaacgtcc cccattatta aagaaggcga tcagcatgtt tctaattttt
     6841 tctctcatta cgttattttt ctcagcttct tacttttcta cctatttgct acttgggatt
     6901 gttttagaaa tagctacgtt gcttcctatt acaaactact tgatgaattt tggaggaatc
     6961 tttcatgaaa aaaacaatca ttaaacaatt aactaactta tctgactttt ttgtatctgg
     7021 ctgctgcttt ggtatgagtt ctgaattcaa aatccctgaa aaattaaaaa aataatacgc
     7081 caattgtgct ataatttggt aaaaatcaat gggagctgta ctttatgttt ttatggtcca
     7141 tagcgttgca atatttgatt tacacaattt gtatcttatt gattgaccat cacatccgat
     7201 acaaatatct gtttgcggga gtcattctga tttcttgtat tttgattgga tgggtctatt
     7261 cgattttagg gatttttact gctctaattg ctataggaat gctgatgctt acttttttct
     7321 tcttttcgac ttcgcgtcaa caattggttt acgcgctttc tttttgggtt attgacgtcg
     7381 cttttaggag atcacttaac aagcatcggc gatttttttg ttttgaaatc ttcggttatt
     7441 gaaccaggag attcattgca gtattttcac attgtcttat ccgctttttt atctgcttta
     7501 ttagcttttt gtttaaaaaa atcgttagag acttggtttc acactgtgga ccgccgtatg
     7561 atcggtacga tcgggacatt gatattattt acatactata tcattatttt ttatacccgt
     7621 ttttctggtg aaacacctga tgtcttagct ttgaatacga gttttttcat tctatatctg
     7681 tgtgttggcc tgatcatctt ggtttattat tggaaaatct ctaataagaa attagctgat
     7741 cagttattag agcaacaagt caggatgcaa caggattata tcaaagatct agaaaaaaac
     7801 tatcaagact tgagagaatt caaacatgat taccaaaatc tgcttttttc tcttcacagc
     7861 tatatttcag aaggcgactt agaaggatta cggtcttatt atgatcaaac gatattacct
     7921 acacgccaga tgatgaacct ttttccggcc aatctaagta tgttggataa tatgaaagtt
     7981 cctgagatcc gtagtttact gtcattaaaa ctcatgatgg cacaggaaaa agggttgatc
     8041 gtccagcttc attttcctga gccaatcgaa ctggatacga aacatacggt aaaccttgta
     8101 cgtatgcttg gtattatctt ggacaatgcg attgaaggag ctagtgctgc aaagaagaaa
     8161 aaaatcgaat tgaccatact caagaagaat cattccgtga tgattcgtgt gatcaataca
     8221 acaacaaata cattaccgtt aaaccagttg aaacaaaaag ggttctcaac aaaaacaaat
     8281 ccaaaaaacg aaggccttgg cttgtttatc ttggatgaat tgacaaagac caatccttat
     8341 cttttccttg aaacatcaat tgaaaaccag cgtttttctc aaacgattta tatacaaata
     8401 aaataaagga gtaatgatat gattcccatt tatatttgtg aagacgatcc aaaacaatta
     8461 gaagttttat caacggtgat caaaaataga atcatgattg aaaacttaga cagttatgtc
     8521 catatttcta cctctgatcc ggaagaactt ctcaatgcag ccaagatcag aacttcttct
     8581 tttggtattt actttttaga tatcgaacta gaagacagtg aaatggatgg tgtagcgatc
     8641 ggtaaattga tccgtgaatt agatccttta ggaaaaataa tctatgtgac ttcccatacg
     8701 gaaatgtcga tgaaaatctt aaaaagcaac atcgaaccga ctgattatat cgtcaaagaa
     8761 gatctttatg atttaaaaga aaatgtcgag catatcctga gcaaaatttt cgaagatttt
     8821 caaatgtccc aaatccaaaa agatatcttc aaaatagagt ttaatgatga gatcaaattt
     8881 ttaccgatca aagacatcca atatttttct acttcgctag gagcgccaca taaactggaa
     8941 gtccatttga ctcatgccca attacaattc tatgagaaga tcaaagaaat cgagaaaatg
     9001 catcggaatt ttatccgttg tcataaatcc tatgttatca atacacaaaa tgtgcggtcg
     9061 attaataaaa aaaccagaga agtcacgttg gcaaacggcg agaccatccc agcctctatc
     9121 cgtggattaa agaaattgat ttcataaaaa gaaccgaata gtttggaccg actaaatgac
     9181 cagccgatcc aaacccttcg gttctttttt gcctatcagt gcaaaagcat ttcctgtgca
     9241 atttcacgtc catccattgt atctggataa aatgttggcc aattctccat ttctttcagc
     9301 aatacttctt gactttcatt tcctgcatat aaatgaaaat gttcggagtc agccggtgca
     9361 atcgtgtgat cactgaattg aatatatcta aaagcgccac tttccttatc ttctgcttcg
     9421 aatagatacc ttacgccacg gtttcctgcc tcgtaggtca agatttcttt ccctacataa
     9481 cgataatttg aacgtttcgt tgttccattc ttaaacgtaa atgccatctc tttctcactg
     9541 atcttgattg aagcaatatc tgtttgatag cccttcgtat aatattcttt atattcttgt
     9601 gctgtcttat cctgattcaa tttttcttta tattcaaaaa caggatcaaa agtcccatct
     9661 tctacatacg tatctactgt ttgccaagtt cctgcccaat ctgaaagaga gcgatcttgt
     9721 acatcctcgt cttcaaaata accgttgtag acgctctttt cttttttggg ggtcaaagta
     9781 tcttctagcg ggttttctgt ctctgtcgtt tttttcaacg cttcgaggtt tgcttccatg
     9841 atagaaagat agttttctcc attttctatt tgttcattcg tcaatccttc caaaggattc
     9901 aatacttcta aagatacacc tgcttcgttt gctaacgttc tggcaatact atctttggcg
     9961 ttttcttcaa aataaatata gttgattcca tgttctttaa caaagtgttt cagttcagca
    10021 attctcgccg cagacggttc ttcactagaa gaaagaccag caatcgatac ttgattcaat
    10081 ccatattcta acgctagata gttgaatgca gcatgttgcg tgacaaaaga tctttgtttt
    10141 gcttgcttaa aggtttcttg ataaagctga tggagtgctt gaagtttctc ggtataggct
    10201 gctgcatttt ttgtccatac ttcctgtttt tctgggtaag cttcaatcag ctgatcacgt
    10261 atgtttgcta cctgcttgat tgctaaactt ggagctagcc atacatgtgg gtctagctca
    10321 tgtgtatggc cttcttcccc gtgttcatgg tcatgatcat gtccttcttc acttcccgga
    10381 agtaaaagca taccttctgt cgcttttatc gtatggacat tgccttcttg taatgttttt
    10441 tggattgcgg ggacccatgt ttccatgttt tcatcattat aaacaaagat atctgtatct
    10501 tggatctttg ccatgtcttt agcagaaggt tcataatcgt gggaatcagt ccctgctgga
    10561 atcaataact ctacttctcc ttcatccccg acgattgctt ttgtaaaatc atacatggga
    10621 taaaaggtcg tcatcacgct caatttttct ttactgtcct cggctttccc acttgccccg
    10681 caagcagtta acatacctgc taaaaccagc atggccaata tacctatcaa tcgtttcatt
    10741 tatgacactc ctcttctcat cttctttcat ttgaatgttc cgattcgctt ttcgcaaacc
    10801 gtaatagtta cgttttagca acaggattta atttatcata tggttaaaag aactgtcaag
    10861 cgttttctta ttttaattga tgagaaagtt tatatgaatg attgacgata tttttgcaat
    10921 caacctctat cattttcctt ttatacccgc ttttgtctat aatgaagtta tagataaagg
    10981 aggaaaaaga gtgaaaaaag aaacagatac aacgaatcgt ttgatccgct ttttaggagg
    11041 aaaaacctct tactatgttt taggaatagt tatcttactt gcgctgatca tttatttttt
    11101 ccatcagatc tcgtttatat tcaagcctgt cggaatcatt atcgctacta tcttgccccc
    11161 attattgttt gcactgatct tgtattatct tttggaacca gtcatcaaac gtttgagcaa
    11221 gcacctttcg aggaacttgt cagtcattat cgtttatgtg attattttat ttttattggg
    11281 gcttggaagc atatggctta ttccttttct tgaagaacag gcaaagacat tgatcagtag
    11341 tttgccagat ttgtttcaag attttcaaga gagtctccga caatttttgg agaaaacacc
    11401 ttttgccgat tcatttaatc aatttttcgc ttctattgac gacgtgacgt ctgatatagc
    11461 tggatttatt ggaaattatt gggaaagtgg cgcacaacgg atcagcaaca ttttttcaac
    11521 tgttacagct atttttatta ctttatttac tggtccaatt gtcgcttttt tccttttgag
    11581 agatcctcaa aaattttatc agtcagtttt agcgattgtc ccaccagcat ttcgtacaga
    11641 ttttaaaaat ttgacaaaga ttgctaatca acaattgggt tcatttctaa aggggcaaat
    11701 cattgcttca tttatccttg gagcagtcta ctgggtttgt ttcttactga tcggattaga
    11761 atttgctagt gttttagcta tttctgctgg gttgttatgt atcattccat atattgggcc
    11821 gtttatcgcc ttctttccag gtttgatcat tgcattccaa gactctactt ttatggctgt
    11881 caaatttgtc attgtctggt ttgccgtaca acttctccac ggtgacttgg tgattcctcg
    11941 agtaatgggt gatcgtttac agatccatcc gattacgatc ttaatcgttt tacttgtcat
    12001 gggtgatcta atgggaattg tgggcgttat ttttggtatt cctatctata cgttagtcaa
    12061 attactcgtt acttttattt tccgtaaatt caaacaacgc tacaataaat tttatggcga
    12121 taaaggagag tatgaaaact cttcattttc tgaagaagat tattttaaat aaaacacaaa
    12181 acgtcgatct tacgagaata gctcgtagga acgacgtttt gtgtttttgt tttaattttt
    12241 cagcaaagca attgcctggt atggttttag ctccagactt tgatgaagaa cagtatcgga
    12301 ataattatcg ataagaatat tggcttctgt gaatccttct gggatggcga tccttgtatc
    12361 tttatcatag aaatttgtta atacaagcag ctgctgtcct tcaaattggc gaagaaagcc
    12421 ataaacttcc ggatgatttg cttcataagc atgataatct ccttgtgcaa cgattggata
    12481 tgtttttctc agccgaatca atttcttgta ataggacaaa atagaatctt cagaagatag
    12541 ttctttttct acattaatct cttctagctt gcctccaact tttaaccaag gtttccctgt
    12601 agtaaatcct gcttgttctg tgtctgacca ttgcatgggt gttcgagagt tatcgcgaga
    12661 ttttgctttg atccgacgaa acgcttcttt ttctgtgaat ccttgttcaa ggagtaactt
    12721 ataagcattc aaactttcta cgtcaatata gtcggaaatc gatgaatagt ctgggtcgat
    12781 catgccgatt tcttctccca tataaatata gggcgtccca cggttcaaat ggatcatggt
    12841 tgctagcatc tctgctcctt gttttcgata ctgatcgata gctacaaatc gattgagcgc
    12901 acgtggctga tcatgattat taaaaaatag agcattccag ccgttaccct cactcatctt
    12961 ttctccccat gtatgaaata gtcgctttaa ttctgcaaaa tcaaatggag gagtagacca
    13021 tttattccca ttttcataat cgactttcaa atgatggaaa ttaaagacca ttgataattc
    13081 ttctctacta ggttctgtat acaagataca attttcgatc gtcgtagagc tcatttcacc
    13141 gactgtgatg atagtggaat ccgtcccaaa cgttgcttgg ttcaattctt ttagatacgt
    13201 atgggtgatt ggtttgtccg tatattcagg tttgccatca ttttctttat tatttttcaa
    13261 ttgttcgtct ttaccgataa cattgataac atcgaaacgg aatcctttta cccctttttc
    13321 catccaaaaa cgaacaactt tgaatagttc ctctcttact gcttggttcc gccagttcaa
    13381 gtcagcttgt gtccgatcat ataagtgcaa ataatatttc cccgtatctc caaatggcga
    13441 ccaagcactt ccaccaaatt ttgataccca atccgttggc tcttctctta aaatgaagaa
    13501 gtcctgataa tgctggtccc ctgctagtgc ttttttgaac cattcatgtt ctgtcgaaac
    13561 gtgattcagc accatatcga gcatgatttc tatccggtat ttctttgctt cggctattag
    13621 ctgttcgaat tcctccatcg taccaaagat aggatcgatg gctgtgtaat cagagatatc
    13681 ataaccgttg tctttttgag ggcttggata aatagggttt agccaaatca tgtcgatgcc
    13741 tagatcattc agatacggga gtttttcttt gattcctttt aaatctccaa ttccatcacc
    13801 attactgtct ttgtaagatt tgggatagat ttggtaaatg actttgtctt taaatgtcat
    13861 ggatatttac ctccattcaa ctgatttatt acctaccaca acttatatat acaagttatc
    13921 atttattatt ataactgaac cgaaagcgtt tgtaaaattt atctttatac attctaagag
    13981 gtctgttgcc caataaaaaa caagagatca gacatgtatt tgtctatcct cttgtttaaa
    14041 atagcaaaat attcttgttt cttctataaa tggtgttcaa aagcttttat ctacggcaat
    14101 cagtcttctc aaatgtaaaa catgaggagc taattttatt tgagtattct ttttgctcga
    14161 tgcaggacgg cttttttaca acctctgatt catggtgttc aatcagctgt ccttcggtat
    14221 tagctcaaaa aacggcaaaa tatgaaaaac tattttgccg tttgtttctc taatactcag
    14281 gtcaaaccgc tgatttcaca acctctgttt ttattcattc gatttcaacg catcaatcat
    14341 atcgatatgc tttaatttga aatgggtaac aatcatcaca ataaccgtaa atacgatagt
    14401 taatcctgct gataagacat aagcccacca tgtgatcact aatggaaaga tgacattctc
    14461 catagacgct tgttgcaaga tgaagtcagt caatatatat cctacaccaa atcctccgat
    14521 gattcctaaa agagtaaaga tgatattttc tcgaacaatg tacatcgtga cttctttatc
    14581 aaaaaatcca aggactttta ttgtagataa ttcgcgtaca cgctcggaaa tattgatatt
    14641 agttagatta tacaagacga taaaagccag cagtcctgat aatacgacaa agattaagac
    14701 aaccgagtct aagttcgcca ttgattcttc ttgcgtttcg atttgtttgg acatgaaaga
    14761 cgtatttgtc acagcgtctg tggcaagcag tttctcggaa agcatttctt cttcttttgt
    14821 tgtcatctta tctgtacgca gcaaaaatgc attatccatc atcgattgat cagtaactga
    14881 agcataatat tcaggggaca tataaataaa gctacctaga tagtttttca cgatttcttt
    14941 tacttcgact ttccattctt tttgatcttc atcataaagc gaaatatgat ctcctgtaga
    15001 taagtcaaat agttctgcta ctttttgcgt aatagcgatt cctgttttag gcaaggagcg
    15061 tgggttctct tcaagatcag ttaaatggat aaaacgatcc aaatcagctg ttttacttgg
    15121 tacgatcact gtcagacttt gcgaagcatg tccttttttt ctcaattcta cggtttgagt
    15181 atgaatagct aggatctcat ttactttttc ttgcttctgc agcatatccg ctgctttttg
    15241 acgactatct tccgtggatg aatccaatgt aacgattgct tgatagtcaa tgatcgttcc
    15301 aaattgtttg gctgaaacag aacttagaga atctttcaat cccacgcctg caaccatcag
    15361 cccggcacat ccagcaattc cgataatcgc cataagcatt cttgacttat atcggaagag
    15421 atttcggtaa ctgatttttt gattaaagct aaggcgtgac cacagcggag tgatgtattc
    15481 aagaaaaatg cgtttccctg gcttcggtgc tttcggttga agcaatgcag caggccgttc
    15541 tcttaaatct ttgaataaaa cgatcattgc agcgccaaat gcggcaatga agaatgcggc
    15601 agctgcttgt aaaatcggcg gccagtcata aaaaataagc gcactcccaa tatcataccg
    15661 ctcgttagac agctcaaaaa tgatccttgg caaaagattc gttcctaata tcgttcctaa
    15721 aacaatacct atccctgagg ctagacttgc atagattgca tatttttttg cgatttcaac
    15781 tttcgtataa cctaacgcct ttaatgtccc gatttccctg cggttttctt ctaccatacg
    15841 tgtcattgtc gtaaaggtga tcaatgcggc aataaagaaa aagaaaacag gaaaaacatt
    15901 ggcaatcgca gcgatacgat cagacagatc accaaattct tgaaaaccga cattttctga
    15961 acgttcattg tataggtatg atggtttagt taaattttca attttttctg catttcgatt
    16021 caattcttcc tctgcagagg ccagttcacg ttctttctca actaattgcc tttcttgttc
    16081 agcaagttga gcttcttgtt gagataatgt ttgaacagcg attgttcgtt gcgcttcagg
    16141 aagctgctgg atagttgctg actgttgcgc taactgtttt ttagcatctt ctaaccgtgt
    16201 tcttgcttgc gttgtttgtg ctttgccatc cgctattttt tgctgtgccg gttctaattc
    16261 ttcgttggcc gattttttca gctcttctaa gcgttcttcg ggtcgatctt gtaaatactt
    16321 ttctgccttt tcttgatttt gctccatttt atctttgtaa gctttgctgt acgtattcaa
    16381 attctttacg tttgtaaaat caagatataa gacagaataa atatctgttt taaaaacgga
    16441 ttcaggtaaa tagacaaaat aatcgacgct accatttccg acgttcgcgt atcctctctc
    16501 caaattgttg atgaacaatg gagaacggac aaagcccgtg atggtatact ctttttcttt
    16561 caattcatct gaatcaatct gataaatatc accgatttta tacccttcat cttccgcttt
    16621 ttcatccaat acaagctggt ttggtttttc cgggagtttt ccatcagtta attgtagctt
    16681 gttctgcttg ttatttttgt tataagaaaa tatttgaatc acttcgtttt tgccagtatt
    16741 ggaataatag atctgttttc ctgtttctac ttttgcatct gggatctttt ctgcttgagc
    16801 aatatctttt tgtgtcaagc caccagtaga aacgatttgc aaatcagaaa gtcctgctgt
    16861 accaacatag tgatccatcg ttttttgcat gacaggtcca gcagttttga cacctacaaa
    16921 taaaagtgtt cctagcatga caataaggat gattgcaatg aatcgtcctt tagattgccg
    16981 aatttcgcga aaacctgctt taagatatgt tcttgttttc ataaaatccc cctaccatac
    17041 aatctcatca atggatgaag gatgatcatt taattcaaca gaccttactt tagcatcatt
    17101 aatgtgaatg actctatcag cgatgggcgc taaagcagaa ttatgtgtaa tgatcaaaac
    17161 ggtattgccg tgttgtctgc tagcgttttg caataatttc aatacttgct tgcccgtttc
    17221 aaaatccagc gcgcctgtcg gttcatcaca taaaagaagt ttgggatttt tagctagtgc
    17281 acgagcaatt gaaaccctct gctgttcgcc tcctgataat tgggacggaa agttattcaa
    17341 tcgatgagct aggcccactt gcctcaatac ctctacagga tcaagcgcat caggagaaac
    17401 ttccgttgcc aattccacat tttcttttgc tgttagatta ggaacgagat tgtagaattg
    17461 gaaaacgaac cctacatctg ttcgtcggta agcagtaagc tgtttatctg aaaattgcgc
    17521 aatatctgtg tcatcaataa tgatctgacc ttcatcaggt gtatccatcc cacctaaaat
    17581 attaagaatc gttgactttc ctgctccact tggaccaagt ataatcacta attcgccttt
    17641 ttcaattgaa aaagaaatat cgtcatttgc tgtaatggtc gtttctccca ttttgtatcg
    17701 tttgtattca ttttttactt cgacatactt cactctgatt cctccatttt cacgatattt
    17761 ctcgctatat ctatgcatcg aatgcgtttt ctcacactta tttattttac cactgaattt
    17821 ctctagacta aagaaaggaa tcctttaatt tagttgacaa ataaacgatt tatgatttac
    17881 ttacataaaa aacattgtta tttgaaagaa ggaacatcat gaatgattta aaggaaaata
    17941 tgatttttga tttatcaaaa actgcttttg tcgcgatcga catgcaaaaa gagatcgtaa
    18001 ataacggtac actctctcca cacactgcag catctattct gacaaacaat gatttgttag
    18061 taagaacatt gaaacgtacc gctgccttga aagttttagt gaacgtagac atctccactt
    18121 tcccttattt gagtcaacaa actgatatgg gtggaatgac tgcttctgta cctccagaat
    18181 tcactgattt acttttgaag gattctttga aagatacaga taatatgttg actatcacaa
    18241 aatacaatcc aagtgctttt tttggtactt ctcttgatct tcaacttcgc cggcgtggaa
    18301 tcgaaactat cattttaagc ggtgtagcga caacaaacgg tgtatatgct actgcattag
    18361 atgcctttca acacgggtac catatcgtgc ttgctgaaga tgcttgcagt gatcgtgaca
    18421 aagaatccca tcaattgttc ataaaaaaga tatttccgaa aactgcacga gtacgctcaa
    18481 cgaaacaaat aatcgaagct atccaacaaa gcaaataagt actggcacat gtgattttca
    18541 tttacttctt tttgatttca tgggaaaata accacaggat ttagatagga gtatgaacat
    18601 ggtgaaaaaa aagatcacaa tcaaagaagt cgctaaactt tccggcgttt caatcacaac
    18661 tgtctccttg atattaaatg gaaatacgga gaaattcagt ccgaaaactg ttcagaaagt
    18721 tttagctgcc aaagaagaat taggctatca gcctgactat tttgctagac aaatgattac
    18781 caaggaaacg aaaacaattg gtgtactcgt tcctgatatc acgaatccat tcttcagtac
    18841 actgatgcga ggaattgagg atatcttgta taagcagaat ttcgtcacga tattatgtaa
    18901 tgctgattct gatcatcaaa aagaaatcga atatctagcc gagctcacac gtcgaggtgt
    18961 ggatggattc attatcgcga cttctgctgt ctctacagat gcaatcaacg aaaatttgaa
    19021 aaaacaaggc cggcccttca ttgttttgga tcagaaaaaa tctgaaggat ttagtgatgc
    19081 cgtacgaaca gacgattttc gcggtggtta cctagcgggc atgcacttgc ttagtctagg
    19141 acatcaaacg atcgcccttg tctatcctga aaatccaccg gaaaatgtcc acgctcggat
    19201 tgaggggttt aaatctgctc ttgatgttta ccaaatccct cacgaccaac tgatcctctt
    19261 acctacacag ttctcaaaac aaggtggcta ccagatcaca gcagaacttc ttgattctgc
    19321 tgcaactggt gtgtttgctt taaatgatga attagctttt ggattatatc gcggattaga
    19381 ggaagctgga aaaagcatcc ctgaagacta cagcattatt ggctatgata atatagatat
    19441 gtgcgagtat atcaagccga aactgacaac gatcgcacaa ccaatcttcg aattgggaca
    19501 aacatctgcc aagttattgt tagatcggat ccaattccca gaaaaagaat gggaagaaaa
    19561 aagattacct gtacgattag aaaaaagatt ttcaactgca cccttgaaat agctttcaag
    19621 gtgtgctata tttttatcga actattaaaa cgttttacta aaacgtttta aaaaataatt
    19681 ttcccatcaa aataaatgag atagttggag gtttgtaaaa tgaacacggt tacagtaatt
    19741 ggaagtatta atatggacac tacccttcgt ttaaccaaca tgcccaagcc aggcgaaacg
    19801 atgcatgcgc atgagatttt tcacgctggc ggaggtaaag gtgccaacca agcagtggca
    19861 gccaaacgtt caggcgcacg gacatctttt attggaggcg ttggggctga cagcgaagga
    19921 caacagttat tagatctact gacaaaagaa aacatcgata catctgggat agctgaaatc
    19981 caaggcgcca caaccggaca agcaatgatt atggtagacg cagcaggaga aaatagtatc
    20041 ttgatccatg caggggcaaa taatgctttc catgaacaag aagtcctaaa aaacaagcaa
    20101 ctgattacaa acagtgactt tattattgct cagtttgaaa gtagcttaga tgcgacgatc
    20161 ctagcttttt ctattgcaaa agacgcagga aaaacaacga tcctgaatcc tgcaccagct
    20221 cgtgaaacga ttcctactga actacttgaa aagacagata tcattatacc aaatgaaaca
    20281 gaaacagaaa tcattacagg aatcagagtg acagatcata acagtttggt cgcagcagcc
    20341 gaaaaactcc atgaattagg tattggaaca gtaatcatta cactaggcag tgccggcgct
    20401 ttctatcata cagaaaaaga acacggtatc gtccctgctt ttaaagtgga tgccgttgac
    20461 acaacagccg caggtgatac atttattggc gcattaagca gcacacttca gccagacctt
    20521 agtaacttga aagaagcgat cctttatgga aatctagcct cttctgttgc tgtgcagtct
    20581 tatggtgcgc aaccttctat cccttaccgc gaagctttag aacaatcagc agtctaaaat
    20641 taacaatgag aggaagaaac aaacatgaaa aaaggaaaag taatcaattc agatatttca
    20701 cgagtaatcg cacaaatggg gcactttgat aaattaagta tcggggatgc aggcatgcct
    20761 gtcccctttg gtactgaaaa aatagatttg gctgtctcaa acggtcttcc aagtttttta
    20821 tctgttttag aaaacgtttt agaagaactt gaggtacaat cgattgaatt ggcggaagaa
    20881 ataaagacaa tgaacccgga aatattggaa aaaatcaaat tattgttacc agatacaccg
    20941 attagcttta tcccacatga agaaatgaaa aaagagttga ataactgtca tgcatttatt
    21001 cgtacaggcg aaatgacacc ttacgccaat attatattag tgagtggagt ggtcttctaa
    21061 tggaaacgac aatgaatcct gaacagatgg tactgtccga gcagattcat atgctgattg
    21121 aacagttggg aggaaaagac aatattgatt ctgtccttcc atgtgcgact cggcttcgta
    21181 tcaaattgaa aaaacagcca gtagccaatg cagaaaaatt aaaacagcaa atcccttcca
    21241 ttaagtattt cattttactc gatagatatg cagaaataat catggatcag ggattggctc
    21301 ctgcttacaa ggaattgacg cgtcaaatcg atcattcagc tccgaaagaa aagctaaatc
    21361 cgatcgaaaa actatttggt ttgatatcgg aaatctttac gccgatcatt ccgctgtttg
    21421 ccggttctgg tatattaaaa ggtctggttg ttttagcgac tacgattggc tggcttagtg
    21481 aaacaagcgg aacttatcat attttgtctg ctgcgtcgaa tgccgtcttt tattttctgc
    21541 caatctttct tgctttttct gctgccaaaa cgtttaaagt gaatggatat attgcggctg
    21601 tcattgctgc agcactgatt gaaccgaatc tgacctcttt actttcagac agtggcaaag
    21661 cagctactga ttttctgggg attcctgtag tactgatgca gtatacgtct actgtcatgc
    21721 cggcgatttt aggtattttc ttttattctt ttgttgaaaa attcctcaaa aaatatattc
    21781 gcgaaaatat gcaattagta tttgtacctt tactttcatt agttatcacg gttcctttga
    21841 cgttaatggt attcgggcct gcgggtgttt accttggcga aggtttagct gccgcaatta
    21901 cttggatgat ggatattaac ggaccgatca gtggcgcaat catcgctggt ggttggaata
    21961 ttttggtaat atttggtatc caatgggctg taaaccctgt catgatcagt aatatctctg
    22021 cttatgggtt cgaccgaatc gttccgttga caggagcagc caattttgga atggctggtg
    22081 cagctttagg tgtatttctg cgaagtaaac gttcaaaaac ccgttcgatc tccggttctg
    22141 cttttgcttc gattttactt gcgggagtga ccgaaccaac tgtttacgga attgcgatcc
    22201 cattgaaaaa accatttgtt gctgcttgca ttggtgcagc tgctggtgga gctgtaatgg
    22261 gatttgccca agtgaaagcg atcgcttttg tatttggtag tctgaccaca ttgcctgcat
    22321 tcatttccgg tacttttttc tggtacttag caggactggc tgttagtcta gtcgtagcaa
    22381 tgatcactac tctcgttagc gggtttgatg aagacctaat gtcttatgaa tagttaatga
    22441 aaaagactga agtatgagat gtttatcaca tatttcagtc tttttctttt taggatcatt
    22501 atttttataa atttttgtga tactttccaa actttggtat aatttacata tgcataacca
    22561 tttttatccc cacttgttta tccccaaaca aaggtggtct atgcaataaa atgaacgtca
    22621 aagaaaaacg aatgaatgct cgttgcttat ttacaaattg ttttatttag aatacacaga
    22681 cagatttatg aaaagaaaaa gcttttcgac acgattatct gttccaaaaa atgtctaatg
    22741 aatgctgaat aagacagaaa atcaatgaaa ggaaaaggac tagtttatta tgaaaaataa
    22801 aaaaacgata cgtttgctag ctgctacttg gctgattgct ccgtctgttt tgtcgaccaa
    22861 taccgtattt gccgtagata atcaaatagc tgatcaaaca gtcacaacag atacgactac
    22921 atccagctca gctactagtg aaacaacgga ttcttttaca gaagacccaa ctggaacaac
    22981 taactcaaca acaggatcga ccgagccaac tgaaattgat ccggctgatc catcagaaaa
    23041 tgaaaagcca gccgagacat attcccttac aattgctcct tctttgattg gtaagatcca
    23101 actgactgct tcgacagcgg attcccctga acagcaacct ttggatatta cggaaagtgg
    23161 aacggtagaa ggtttattag cggaaacgaa ggtaacttac actattacgc cagctgaagg
    23221 tgaacaaatt gcttcagtcg ctgttaatgg tgccaaagaa gtaaattatg atgcaaagac
    23281 ctttacagta ggtacgaaca atttatctat tactgcagaa tttaaagctg gaccggatac
    23341 ttcaacagag aaaccaactg atcctagtga agaagtccct agtacaccag atccggaaac
    23401 gccaaacaaa ccagatgtgg gtaataacaa caataataat ggttcaaata attcaaacaa
    23461 taataacaat aattcgaaca gcgggaattc ttcgaatcaa acaaacccat ctggatcgaa
    23521 taacaatgga aatactcaga gagaaccaaa ccatccaaac aatggttcat cgaatggcgg
    23581 aacgaacaat gcttcatctc gtccaaatac agcaaatcaa agcggtatag agaatccatc
    23641 ttctgcttct tctgattttg ttgtaaaatc accaattgat gctgtattgc ctacaaatac
    23701 aacgaatgtc caacaagcaa tcgttcgaga agcattcaag catcttggaa aaccatatgt
    23761 atggggagca aaaggtccaa atacttttga ctgttctggt ttgacttatt acgtatatat
    23821 gaaagcaacc ggtcattata tcggtggctg gacaggcgaa cagcaatatg caggtacgca
    23881 gattccagta agtcaggccc aacctggcga cctcgtcttc tggggacctt cttccggtgt
    23941 tactcaccat gtaggtatct atattgggaa cggacaattt atccatgcgc ctcaaccagg
    24001 agataaagtt cgagtaactt ctatttcaga tttcacgcct gattttgctg tccgagtaaa
    24061 cttagcaggt ttacctgctg cttcaggttc gctgacaggc ggctcatcta ttttagatgg
    24121 attagatggc tcgttccatt tcagtcaaaa ccaaacaact gatcagttct tgaaaaagat
    24181 tgcagatgat gcacaggaaa tcgggcaaaa agaaggaatc tacgcatcag tcatgatggc
    24241 tcaagctatc cttgaaagtg gatctggaaa cagcttatta tcaagcgaac caaatcataa
    24301 cctttttggt atcaaaggat catataaagg aagcagcgtg accttcaata cacttgaaca
    24361 agatagttcc ggacaaagct accaaatccg cgcacaattc cgtaaatatc cttcttacaa
    24421 ggaaagtttg gaagactatg ccgacttgat caaaaatgga ctgactggca atcctgattt
    24481 ctacaaacca acttggaagt cggaaacaaa agactacaaa gaagcaacca aatatttgga
    24541 aggacgttat gctacagatc gccaatattc acaaaaattg aacgcgatca tcgaagcgta
    24601 cgacctgaca aaatatgatg aaccgaaaaa agaagcagca acaactgaag aagcaaacgc
    24661 tgaaatcctc aatatgtcta agcgattcga tgtgccaatc aaatggacaa cagatggcgt
    24721 gccaatcaca ggattagaat tttcaacgat aaaacgcgtc aacaacgctt cttccatctt
    24781 ccgtgtcgct tcaatctggg atttgtggaa caattttacg gcaagacaga taccagaaac
    24841 aactactcgt acagttaaag ctgcaactgt tcctgtacta accttgttaa gtttagacaa
    24901 atcattctgg cttgatctgc ctttaaagaa atagaataaa ttaaaaaggg gatgtgacac
    24961 tgtcacactc cctttttaat ttattctatt tctttatatt tattttgtat tccgttacaa
    25021 actcgcgtac ttggttaggt tccagagtga catctccaaa actttcaagt cgctccgctc
    25081 ctggcagttc ttgagtctca aatgtgatac cgccatgatt tactaattta ttcccgcgca
    25141 tttccggtcc atcttctcca aaatttgctg taaaaatcac gattcctgaa gcatctgttt
    25201 ttacttggat cgatactttt tcatctgggc ttgtcaattc cgcagcattt agtaaaccag
    25261 attgtttttt caacaaaaat ggatgatcga tgccgcccac tatcattgat tggcgaaaat
    25321 cactggcaaa gacctcacct aattttttag gacgttggaa gtcaaatgct gtgccactta
    25381 ctgaaactac ttctcctgta ggcgttgtat ctttattgag cgtagcaaat aatcggctat
    25441 tcaaccaaag tatatgttcg tcgattgact gtgctggatc accggttaaa ttgaaataga
    25501 catgatttgt aggattgaat actgttttgc ggtcactgat cccttggacc gtcactcgcc
    25561 atgtatcatc gttcgtcagc gtataccgta cttctacatc taaagttcca ggaaatccgt
    25621 tttccatatc tgggctagtc tgatagaaaa tgaccgatgc ttcgttttca ccattgatga
    25681 ctgtataatg ccactgcttg gcttcaaagc taggtgcacc accatgtaaa ccaatgacca
    25741 gtgtccgggt ctgtttttac ttcgaacgtt tcatcatcta atttgaatgt gccattcgta
    25801 atccgtccag ccatacgtcc gattgaagca ccgatataag ggtctttttc tagatattct
    25861 tttgcttcat caaaacctaa gatcaactct cgtttttcat tatcgattat tgtttgaaaa
    25921 tgaacaaccc gtgccccaag atcactgaca gacaaagatg tcccattttg attcgtgatc
    25981 gtaatcaagt gatagttttc gccaaaattt tgttcattga tttccatgtt ccttgcaccc
    26041 tcttttcttt catttgcact gattccagca tacatggaaa ccgttttctt aacagcttat
    26101 tccgtgagta acgagaaaaa atttactata ttctaataaa agaccgtgaa aaaaaggttg
    26161 tgacaaaaat attgtcacaa cctctttttc cgaacaaacg gtgttcaaaa gcttgcatct
    26221 gcggtaataa gcccaaccaa acgaaaaata tgaggagcta tttttgcttg gctgttctta
    26281 ttacttgatg caggacagct ttttcacaat ctctttttca atacgattcc agtatataac
    26341 catgtcgtca gtacatagta gatgaaatac aatacgaaga atatcgtaaa cggaacccaa
    26401 atatctttgt atggattttg catgaataga ccaaacattt gtaatccgaa taagacatgg
    26461 actacaccta agattcctgg tgctaagaat aacacgccta cttctctacg gatcgattgt
    26521 ttaagcaacc cgcgtcgtgt tcctatcttt tcaagcattt cataacgagc aatgtcactc
    26581 ttgcttcctg acaagatctt gaacatcaaa cagctagcaa gcatggtcaa gaaagctaaa
    26641 cctaggaaga atcccataaa ttcaaaacca gagaatagac cattatacac ttcatacatt
    26701 tcatatttct ggttgctgta ctctactttt tgcaaagatg gattgttcat tttattttga
    26761 gcaacaagtg tttcgattgg tttcaggttt gcttggaagt cttttacctg aactagctgt
    26821 aaagtggatt gtggcaagtt cagttgatca aattctgatt gactgactac cttgatttct
    26881 ttttcttgct gttccggcaa ttcatagctt cgaagttcct cttgtacagt cagttcattc
    26941 atttttttag caggaacttt ttctttcgtg atcttcggcg gtgttccatc ttcatgtttt
    27001 atcatgatca atggttgagc cgcaaattca cttgcattat agtaaatcgt tcccgcatct
    27061 tctttttgag tataagaagc agaaagtgtt ggtgaaagtt ctttgatttt ttgctgatct
    27121 tctgcccgag gattattcat cactagatca tatccagaat tgttttctgt caagtctgca
    27181 atttcatttt taaaacctaa tcctaccgtc aaagcgccca gtgccaaggc aaacagcata
    27241 gcgaccattg agagcatttg tgtatattca cgaatacgaa agtttaactg tgacaatgtg
    27301 aaattgttca attttttcaa tgaaatctgt tctgatttct ttagtaatga taaaaagaaa
    27361 atgatcacag aatggaaaat gaaataagtt cctaatacga tcgtaactaa ggctacccct
    27421 ataccaaaga cttgataggt ccccacgtgt ttcatcatat agtaaccgca gcctagaaaa
    27481 gcaattccca gtactacttc aaggaaaaac aagaatttat gctgcttgat tcttgttggt
    27541 gtctgatccg cacgaatcaa tgctaaaatc ggttttttga caatcgaaaa agcattgaca
    27601 acagcagcta ataaaaacaa aacagcaaaa aacaaaacag tgatcaacaa cccgtgaata
    27661 ctaaaaggtg taaaatgcga aatattgaga tctaactgat ccactaataa tcggtggacg
    27721 atacttgtca atcctacacc gacagctgaa cccactaaag tagctgcgat ccctactaag
    27781 aatgtttcag aaaaaatcat ctgtgcgatt tttctgcctt ttgctcccag catcataaac
    27841 attgcatagt ctttttgccg catcgtcatc aaaaaggaat ttgcatataa aatataaacg
    27901 aatgtgatga tccctagtaa aacagaaccg aatcggaaaa tgataactgt agccgcaacg
    27961 atcgtattac ttttcaaaaa agcttcgttg cttgccattg attcaaacat gtaaaaaata
    28021 gcagatgcca taactaatcc tgagaataac accaagtagt ctcttaaacg gctcttgatc
    28081 ccagtaagtg ataatttcca aagcatagtc aatctccccc tactgttcca agtttccaag
    28141 aatagatagt atttctcgat agaaggaatc acggctttga tctccacgtt tcacttcttg
    28201 atggataccg ccatccttga tgaataaaat acgttggcaa taacttgcag aaaacggatc
    28261 atgcgtcaca agaagaatcg atacatgatc ttttgtattc aattcgtcca tcgtatctaa
    28321 aagatcccgg gcacttttgg aatccagcgc acctgtaggc tcatccccta atagaatcgt
    28381 tggttgtgtg atcaatgcac gtgcggcggc gacccgttgt ttttgtccac cggaaatctc
    28441 agaaggatag ctttccaaaa tatgagagat ggataaacgt tctgcaattt tttggacacg
    28501 ttgcttgatt tcttttggtc tgacgccttg taaagaaaga ggaacagcga tattttcttg
    28561 tgctgtcaag ctttctaata aattgaaatc ttggaaaata aagccaatct cattcgcacg
    28621 aaaatctgct agttgatttc cttttaatgt ggtcacatct ttttgattga ttcggatatg
    28681 cccatctgtt ggtttatcta atgtggataa aatattcaaa agtgtcgact taccagaacc
    28741 agaagcgccc atgatcccaa taaattcgcc tttttctaca tcgaagctga tgccgcttaa
    28801 tgcttgcgtc tttttctcat tgttttttcc gtatactttt gttacattct gtacttcgac
    28861 tattttgttc atttctttcc ctacccttta attagttttt ccagtgctgt tttcggcacg
    28921 tctttttttt cgacttcttc atacgaagtg acacctttat ttttgttcca ggtgattttt
    28981 aaatatgctc ctttacgaag cggtcgagtc aattgtccct tgaagtccag ttctttttcc
    29041 ttaccctctt tcgtatagcc aggcaggata tatctatagt tgactgtttc ttgtccagag
    29101 tctgattttg aaactgtttt tgtaccatct gtcgtaattt gtacatagta ttcttctcca
    29161 cccataacta cgtaatctgc gattttcacc cctgcaagta ccacaacgat gaaaacaagt
    29221 agaccgatca gaagtttttt cactttttcc ccttctctct tactttatat actgattata
    29281 gaaaaaagcg aacaattctg ccttcgaatc aactatcatt cttctttcaa ttttgtcatt
    29341 ttctttaaaa caactaaaaa aagagttcaa gacaataatc cgtcctaaac tccttcttcc
    29401 tatcttacaa aaagtatgtc acttacggtc tttcatgtcg cctgtattga taacaggttg
    29461 cgttcctttg tctgtaatga ttgagacaag taaattattg atcaattgga ctttccgatc
    29521 atcggtgaaa ttgatttctt gtccatcttc gatttgctct aaggccattt gagtgattgt
    29581 gacagctcct tcaacaatgg tttgtcgagc agacaaaatg gcttttgctt gttgacgttg
    29641 gagcatggca ctagcgattt ctgttgcata ggctaaatgg ttcaagcgtg tttcgattac
    29701 ttcgacacct gctactgcta aacgctcttg cagctctttt tgcaactcgt cagacactgc
    29761 cgtcgtgttt cctcgaagcg tcaaatcatt ctcattgaat gtatcatacg ggtattggct
    29821 agcgatatgc cgaatggctg tctcactttg gatttctaca aaatcttgat aatacgctac
    29881 atcgaataat gcctttgctg tatcaacgac tttaaagact actactgctg agatttcgat
    29941 ggggtttcca tccaaatcat tgacttttaa aacagaacta ttgaaattcc gaactttcaa
    30001 cgaaacagtc attttttgtg ccaaaggtat cgtaaggaaa aagccatttt ctcgaatcgt
    30061 acctaaatat tgtccaaaaa acaaaattac ttttgcttga ttaggactga ctacagttgc
    30121 cgaacttaac aacaataacg tgatcaacca aagaaaaata cttaaaaaaa gtgcccaaac
    30181 tttcgcttgc cacataccta aatagaagaa aaacaatcca actaaagcta atacgaccaa
    30241 tccgacaatc cctgcataac cattgatata aaatgctctt ttttctctca tgctacttcc
    30301 tgcgctcctt tatccttcaa taaatatcta atgttacttt tttaattttt ttttcgaata
    30361 cgcgccatta caccttctag ttctaactct ttgtcttcag tgatgtacca accgcttgcc
    30421 tgtgtccctc cggctgcctg cggtaaataa tccaattttg ttcgaaccca gtgcttttct
    30481 tctgtaaaat agtcttctac caagacctct agatccatcc ctgctgaaag aggcaaatct
    30541 tcccgaacag atttaaagat aaatcgttcc ttcttcccat catacttcaa tattcctgta
    30601 tcgataatcg gtaaagaata ataatccagc tctttcttca ccgtcaataa atgtttgaaa
    30661 acttcatggt acacttgctc taattggcga ccttctgaat tgttcgggta attaaccatc
    30721 actttttctt tgtctggatc atagaaggtt tggcttagtt gagtgatata agtatgtaat
    30781 ctttccattt ctgtttttaa cttttctctt tgaccttctt ctaccttcat atactctttc
    30841 accctgtttt ctttttagat attttctttc ttattttact atagttatgt gcgttaagcg
    30901 atgaaaaaca gcaaagattg gaaggatctt tgctgttttt tcttaaaata tccagttgag
    30961 tacaggatca atctgtttca ttgtcctctt gatccgtcgt aatattgatc gatgtgtctt
    31021 tagcagtgat ctttagcttt ttctgtcctt gttctaacaa agtatttgtt attggctggt
    31081 agtctttttc aatataagat ttaccagacg tttggatagt atagccactg ttttccatct
    31141 catgcaaaga taaattgcca ccgtttagga tgacttttcc ttctttgctg atcaaactgt
    31201 cttctagatg gaagttgctg ttttcacctg tgacgatcaa gttatccaca ttggcctcat
    31261 ctaatcgaag tgagcctaat tcatctttcg cagttattgt tcctcctgta aattcattga
    31321 acgataccca gccatcatgt gtcttagtat tcactttttt gattgatact tcgttgatat
    31381 caattgcact attattccct tcgattgtca attcagataa ttcatgatct gcagggacaa
    31441 caatagtgat ttgtgcactt gtgtcttcta aactaatgat tgcacctgtt gattttttga
    31501 ctgcattcac agtcaacacg ccatttttct cctttatttc tggcttttct gtcttatcat
    31561 aagtcgtttt tatctcaaaa tccaatccgc gtatgaccga aactgtttga ttttctgcat
    31621 ggatgacgat tttgtttgca tccttgattt tttttgtctc agtaaccata tcgatcaacc
    31681 gaggaccatt atcccaagtc agactctttt gagcgcctaa tgtataagtt gcacctgcta
    31741 ggaggccacc agtaagcatc agaccaatac cggtaatcaa aaaatgtttc aatctcaatt
    31801 tatgtgtcct ccttcaaacg cccgctgaat gcagatctct ttttgtaata aaacgtcgtc
    31861 ccatccattt aacaaagttc atcatgacat aaaaaagccc ttttgttcct ttcagcaaca
    31921 gtggcgaaat aaatactgcg cttcctgtgg cgaacagacc gacaccgata aagaataatc
    31981 cactcagaat cgattgtcca aacacactga ttcctgagat gatcatgaat aggccaacac
    32041 ctaagatact gatacataaa acatagatac taaaaatgag cgctagtaaa gcaaccaata
    32101 gtcccccaat aaccaatata aaccccagtg ctagtggaat gaggacagga gaagcaaata
    32161 aaccgataat gattaaccaa atcaggcgaa tttgtctttt tggatttttc actaactcgt
    32221 tttctgcttg ttcttcttct gaaaagtaac tgatttttaa ttttaaggca aactgctttg
    32281 gtgtaccata ttcatcaatg atctgatcta atgtttttcc agaatctaaa aaatattcct
    32341 cataatactg gatcaaatca aaacgatcgt cttcaggaat ttcttgaaga tgatccacaa
    32401 cttcattcaa atattcattc atttgtgctc tcctccaata accggtctaa agaatctcga
    32461 aaaacaatcc attcatcaat aatttcttct aaatgacgac ttcccattgg tgtcaacgag
    32521 taatatcgac ggtttctgcc ttcaaaaggt tcatcatagg tctcaagata tccttctttt
    32581 ttcaatcttc tcaaaaccgg atatagtgta gattcactga cagagagact ttcttgtacg
    32641 atttttgtta aggcataccc atacatatct tccttgctta atgctgctag cacagtacca
    32701 tcaagaagaa cagttggaat ctggatgtgc attacgtcac cttctattct caagtgtaat
    32761 attttatttc aatctatatt atacaacata taatatagtt tcaaaatcag tttgtcaaat
    32821 acaaaaaaac tgactaaaag atttgatcct ttttagtcag caaaaaatgg cttctctttg
    32881 atttttatta taacagtggc ataatccaat ttgtccacat ccagctcagt ggcatgataa
    32941 cgatcattgc aggtatcatg tcagctactg ggaacatttt cacgttgatc attctaaaac
    33001 cggtagcgac catcaagaat ccgccgcaag ctttaaaatc taagatcatg tctggtgtag
    33061 tcaatggata aataaatttt gccaataaga acaaaatata aaaaataatg aattggggaa
    33121 ttgctatcac agaaacgaca taccctaaat tacacgcaaa aatagctgct gtgaaaaaat
    33181 caaggacaga tttagaaatc aaaatcgtac tgtctcctgt catcccagaa tctaaacttc
    33241 catagatccc cgtaccactt gcacaaaata gaacaatgac cgtcactaat gtgctgataa
    33301 attcatcgtg ggtcatacca agcttttcac tagggaagat tttagaaata ggtacttgca
    33361 tcagcgcagc ccccttattt atcccattac ctaaatgaac aatcaaacca atcgctgttc
    33421 ctatcactaa agcaaaaata acagccggca tatttttcat cggcgcaatg gaatagattc
    33481 ccatccccat cgaacacaca ccaaaaacca tcgtcagctg tgctttaaaa tgttcactta
    33541 atttatttcc tactaatccg ccaattagtc cgccaaacaa aacggaacct acattgatca
    33601 aaactccagt tggcatcaaa aaactcctct ctttttctac gaagcgaaaa aaatcagaaa
    33661 aatttctgta attattgctt tcgctgcaag aaagattgta acagacagag aagattacac
    33721 caattcattt tttgtgttaa agtattcttt attggaataa gtaggaggaa tatgatggat
    33781 atccataaat tcgaagtttt tcttgattta gccgaaacaa tgaatttcac caaaactgct
    33841 gatcgacaat tcaccaccca aggaaatatt tctaaacaga ttctttcgtt agaaacagaa
    33901 ttagacgtca aattattcga acgcgccaat agaaaaatcg aattgaccga agctggaagc
    33961 ttattgctcc catatgtaaa aaatgtcgta gaacaatacc atgctatgca agaagtgctt
    34021 gaaacctata cgaaagacaa caatctttca ttaaatatct taacgatacc gacaatgatc
    34081 aattataaag gtttttccaa aatcaccgag tttttgaaac gccatcctga atttacagtc
    34141 cagttgaaag aagtggaaag tgtcgaatta acttacaatg acgccaatga acatagtgac
    34201 acgatccact ttgctagaag tttccaggcc ccaccaagtt cgattgagtg gttgcctact
    34261 gaaacagacg attttgtagc agttctacct aaaaatcatc ctttagctag taaaaagaaa
    34321 ttggatctaa gcgaactgaa aaaggagaac tttttgctcc ttggccccac tacgaacttg
    34381 tatcaacctg tactagatct gtgtcgcgaa gctggatttg aaccaaaaat ctcttataaa
    34441 ggagcaagaa tcgacttgat catcaatatg attgcaaatg acatggggat tgctattgtt
    34501 atggaaaaaa cagtcaagaa tctattaaaa gaaaatactg tgattctccc tatttctcca
    34561 acaacagaga gttatcttgc atttactcgt aaagtcggtg agcattcttt ggcttcagat
    34621 acattttggg agtatttaaa tcaagaagaa aactaagccc tacattttct gtccatctat
    34681 ccacccaaaa aaagagagcg acgcatgact ttcgctctct ttcttgcatc atgtccttgt
    34741 ttcaatgaca cctctaacat ttctcgtagt tcaataagaa agcagcgatt tttatgccaa
    34801 aagaaccgta agaacatttc cctaatcaca aaaaaacgac taaaaacccc taaaaacctt
    34861 caatagaatt tttaggggtt ttattctttt tcctattgtt tacttaaccg tatcattgct
    34921 gtcgttccta ctccttcgat actgctataa tcaatacgtc catcatgcag ttctagaatt
    34981 tttttaacca gtgaaagtcc tagcccattc ccagaaattt gacgacttgt atctccttga
    35041 taaaattttt caaacattcg atctctggtc tcttcattca ttcctacacc tgaatcggtc
    35101 acttccagta caatctctgt ggctgtctca aatagtttga tatgaatctg tccattgatt
    35161 tgattgtact ttatggcgtt atccatgata ttcagccaca cttggtacaa aaactcttca
    35221 ttgcctgtgt aatttactgc agcaagttcg atatccaatt ctagctgttc tttctcccat
    35281 ttaggctgaa aaaaaacgat tacttctctg atttgttcat ctaatcgata ctctttcttt
    35341 tccagttgga tacgctggtt ttccagtttg ttcaatttta agatgttctc tgttaattgt
    35401 gaaagctgcg tgatactttc gataatccga tataaaaata tttggcgttc ttcgtcagag
    35461 agatttgggg cttgtaacaa ttgaacatat ccttggattg ttgctagtgg tgttttgaat
    35521 tcatgggata cactggacac aaagtcattc ctcagtgtct cgatactatt gagttcttgg
    35581 accataacgt tgaaatcttt atacagttgc tgtacttctg ctactttctg ctgttcatcc
    35641 attcgaatcg aaaaatctcc cgttgctacc agactcatat ttgtccctag cgcactgatt
    35701 ggatgcagga tacgccgccc aacaaaaatc gatattcctg tacccacgac catactgaac
    35761 cccgcaaaga cgaataacgg gtataacgtt ggttttccag ccgattcagc aggcgagata
    35821 gaatgtcgag acaataagaa aataaaaccg agaaaacaca gcagtgtcac taagatcgaa
    35881 atgaagacaa tactagcaaa atagatccat aattgtgaaa atagcttttt tttcatcttt
    35941 aaataaccgc cttatagcct agtccccgta ctgtgatgat ttgaaaatct tccgtctttt
    36001 caaaacgggt ccttaaacgt ttgatatgta catcgatcgt tcgttcttct gtattcgtat
    36061 ctaatcccca aatatcatcc atcaactgtt ggcgagtgaa gatcttgttg ggataagaca
    36121 gtaatttata cagaagcaga aattcttttt gaggcagaat caacgaatgc tgatcttgta
    36181 acactgtata agcatctagc tctaattttg tttgtccgat tatcaagcta ctgctgtaat
    36241 tgatctttga acgtcgaagc aatgcttcca cacgtaggat catctcattg acatcgatgg
    36301 gttttgtcat ataatcatct gctcctaatt tgaacccgcg tttcttatcc tcaaaatcgg
    36361 cctttgccgt aatcattaga acaggtgtgt caaatctggt gctacgtaat gagtctagca
    36421 agtcatagcc atccattttc ggcatcatga tatctgtaat gattaattct atacgtgtcg
    36481 cttctagttt tttcagcgca tcctctccat tcaatgcggt gatgactgaa aagcccgctc
    36541 gttccaacac agaacggtaa agcaactgta attgcggatc atcttctact actaaaatac
    36601 gattcattga tctccctctg ttttttgaaa aatttctaga ttttttcttg tagttcatct
    36661 tcagttaaca tttatttcgt atactacgat cattgttctg atatacttat tagtataccg
    36721 aatatttcaa tacgggagaa gggtgattat gcttcaatta aaagatataa aaaaatatta
    36781 taaagtcggc gaaaccacca ccaaagcatt ggatggtgtg tctgtcgctt tcagacaaaa
    36841 agaattcgta gcgattttag gtcctagtgg ttctggtaaa acgactatgc tgaatgtgat
    36901 tggtggacta gataattatg attccggtga tatggtaatc aacggaaagt ctactaaaga
    36961 ctttaaggac agtgactggg atgcctaccg aaacaactct atcggtttca tttttcaaag
    37021 ttacaacttg attggtcatc taggcattat tgaaaacgtt gaacttggga tgacattaag
    37081 tggtgtttct aaagacgaaa aacgaaaacg ggcagaagat gcattgcacc gagtcggctt
    37141 gacaaatcat atgcataaaa agccaaatca gttatctggt ggacaaatgc agcgagtggc
    37201 catcgctcga gcactagcca atgacccaga tatccttttg tgcgatgaac caaccggagc
    37261 gttagataca gaaacaagta tccagatcat ggaattgatc caagagttat cgaaagaaaa
    37321 attagtgatc atggtcacac ataatcctga actggcccat caatacgcag atcgaatcat
    37381 tgaattttct gatggtaaga tcttaacaga ttcccatcct cacatcgaac gaccaaaaga
    37441 tgatcaattt aacttacgca gaacaaagat gagtttttgg acagcgttaa agctttcctt
    37501 caacaatatc cgaacaaaaa aaggacgtac tttcttgacc tcctttgctt ccagtattgg
    37561 gatcatcgga atcgcgatcg ttctgtccct ttcttctgga ttccaaaaac aaatcgataa
    37621 cacgcaagct gaaacgatgg cgaaatttcc gattactatt tctaaggtaa cgactaacca
    37681 gactcgtgac gatgccggat tgggtgcaag caaagcggat tatccagaca gcaaaacgat
    37741 tactgcaaaa gtcagcgaag aagataaagc acagcacacg aataagattg atcaaacata
    37801 cgtcgattac gtaacagaca tcgatcctaa cttaagtaat aatatcggat ttacccgtac
    37861 tacaggaatc aatcttttac gtgatgtaaa tggcaaagta cagcctgtca gcttctcgaa
    37921 tcaaaatcct gatacggaat ctctttcttt atctagtacg atgtcagcaa tgacaggtgt
    37981 gggagtgtca agtttcccta cacagcttga cactagcaaa gaaaactttt taaaagacaa
    38041 ttattcttta cttgccggaa gttatccagc ctcagcaaca gatgtcgtgc tgatcgtaga
    38101 cggcaataat aatactaata tcaacgcatt gaagaatctt ggatttgatg tgaaagaaga
    38161 cgaaaaactt gattttgatg atatcgttgg tacaacattc aaattagtaa acaacaatac
    38221 ttactataca aaacttccaa ctgggaattt tatccctaat acagattatg atgcgatgta
    38281 tcagaatgct tctgatgaat tgaagatctc ggggatccta cgcgtgaaat cttcttctac
    38341 aatgaatctc ttatctccag gaatcgctta tagcgatcaa ttaacgactc aaatcgtcaa
    38401 cgaaaacaaa gaatcagaaa tcgtcaaagc tcaaagagac agtgatgtaa atgtcttaac
    38461 tactgaaaaa gttgacgaaa atgccaaaca aacattgctt agttatttag gtggagacag
    38521 cttgccttca agtatcatga tttatccgaa caattttgaa gacaaagaaa agatcttgga
    38581 ttatctagat gattacaata aaggcaaatc agatgaagat aaaatcattt atactgattt
    38641 agctggaacg atgactgaac tgactggcgg attgatggat gctatcactt atgtccttat
    38701 tgcttttgca gggatctctt tagtaacaag tatgatcatg atcagtatca ttacttatac
    38761 atctgtgatt gaacggacaa aagaaatcgg tgtactaaaa gctttaggtg cacgtaaaaa
    38821 agacatcact cgcgtatttg acgcagaaac gtgtatttta ggtatttctt ctgggatatt
    38881 aggtgtgttt attgcatggc tagctacgtt ccctatcaat agcatcttat acaacatgac
    38941 ggatctaaaa aatgttgctc aactaaatcc tgtccatgcg attattctag tgatcgtctc
    39001 tactatattg acgatgcttg gcggtcatct tcctgcacga atggcagcga aaaaagacgc
    39061 cgctatcgct ttgcgtgcag aataaggttg tgacaaccac atttagattt gagtcataag
    39121 agcgctgaaa tagtagaaaa cagcaagaaa cgaggaagta atatgttcct tgtttcgcgc
    39181 tgtttttttt aattattttt tataattctg gtatactttt ctttcctttg taccacaact
    39241 cttctggcgt atacctgtct tttccattta tgaatactgt tccatctgct tcaatcgtta
    39301 cttcagtccc aatccacata ttacgagaaa gtgcgagaag attttttctt tttcctcttc
    39361 ctttttttcc aaattgcaag gcatcggtta tctcttctgt ccaatgagat ttcacgtatt
    39421 ttacttctcc gttttcaaat gtaactttca aaatattcgc tatattttcc acttttactg
    39481 ctttaggaaa tgactcctcc atttttttca ctttccttcc tccaacacta agtatgcgct
    39541 ttcttttagt gataagtata tcatagttta ttctattatc aaaaaaacgc atccattcca
    39601 ttgtagaata ctttaccgaa caagttgaat tgttttcctc tttttcacct tttactatag
    39661 ataggtaaag acaactaagg aggaatctct catgacagat caaccgtatg atttaagaaa
    39721 agtcatcgaa gaactaaaaa cacttccagg tcaatatcac gaaacagata ttgaaatcga
    39781 tcctgaagca gaattatctg gtgtttaccg ttatatcggt gctggcggta cagtaaaacg
    39841 tccgactcaa gaaggaccaa ctatgacttt caacaatatc aaaggtttcc ccggcacacg
    39901 tgtgaacatc ggaacaatgg ctagtcgtaa acgtgtaggt gaaattttgc accatgatta
    39961 tcgaacactt ggtcgtttct tgaaagacgc tgtagaaaat ccaatcaaac ctgtgaaagt
    40021 agacaaagca gacgctccag cacaagaagt cgttcatcta gctaccgatg cagacttcga
    40081 tattcgcaac attttgcctg cacctacaaa tactcctgaa gatgctggtc catacatcac
    40141 aatgggtgta gtcttaggat cagatccaga aaaaacaatg agcgatgtaa cgatccatcg
    40201 tatggttcta gaagataaag atacgatcgg tatgtacatc atgcctggcg gacgccacat
    40261 tggtcacttc caaaaacaat tcgaagcctt gaataaacca atgcctatca ctatcaatat
    40321 tggtttagat ccagcaattg ccattggcac gactttcgaa ccacctacca ctccacttgg
    40381 ctacaatgaa ctatggtgtg caggagcttt gcgtaacgaa cctattcaaa tcgttgatgg
    40441 tatagcagtc gatgaagtgg gtattgctcg tgctgaattc atcattgaag cagaaatcat
    40501 gccaaatgaa acaatgcaag aagatatcaa cacgaactca ggaaaagcaa tgccagaatt
    40561 tcctggctat actggggatg ctaatccagc tgtacaagta gtgaaagtca aagcagttac
    40621 tcaccgaaaa gacaatccaa tcatgcaaac aacgattggt cccagtgaag aacacgtatc
    40681 tatggccggt attcctacag aagcaagtat tcttgccttg gtcgataaag ctattccagg
    40741 aaaagtagta aatgtctaca atcctcctgc aggtggtggt aaattgatga ccatcatgca
    40801 aatccgtaaa gaaaacgaag cggatgaagg tattcagcgt caagcagcta ttcttgcttt
    40861 atcagccttc aaagaattga agacagtcat tttagtcgat gaagatgttg atattttcga
    40921 catgaacgat gtagtttgga ctttcaacac tcgtttccaa ggagataaag atatcatcgt
    40981 gatcccagga atgaggaacc atccattgga cccatcagaa ttaccagaat ataatcctgg
    41041 ctctattcgt ttccgtggga taagtgcaaa aactatcttt gacggtacag ttcctttcga
    41101 tatgaaagaa cattttgtac gtgcaaaatt ccaagaagta gaagattggg aaaaatattt
    41161 gaaataatga atgatctgca actgttttga catcataaaa aggagtgcgg aaaatggaac
    41221 aattggcaca acggaagaaa aaaatcgtga ttggtgtaag tggcgcttca ggcaccattt
    41281 atgccatcaa cctagtgaag aaactaaaag aatatccgat gatagaaaca cacggaatca
    41341 tcagtccatg ggcaaaacag aacttaaagc tggaatccga cttatcacta actgaattcg
    41401 aaagcctgtt tgattaccat tattcaaaca aagatttagg tgctgctgtc gccagcggat
    41461 catttttgac cgatggaatg atcatcgtac cggcaagtat gaaaacagtc gcaggtatct
    41521 ctgttggttt aggcgacaac ctgattgcac gtgctgctga tgtaacaatg aaggagcaac
    41581 gaaagttagt cattgttcct cgtgaaacgc cattaaatgc cattcacttg gaaaacttga
    41641 ctaaattagc acgactaggt gtgcaaatca taccaccaat tcctgctttt tataatcagc
    41701 ctcaaacgat ccaagatctt gttaatcacc atacaatgaa acttttggat caatttgaaa
    41761 ttgtaaatga gcattcaaaa cgttggcagg gtgattaata tggaaaaaga aacgatagtt
    41821 gaagtaacca aactaggttt cacgatccaa gctgctgtta aagtaatcgg taaagactta
    41881 ttgatcactt taacaggcgg agatacacca catatcggaa cagtcactac attttctcgt
    41941 gatacagaga tgcaaactgt acgtttccct agccacgatg gtcggttcca taaagacgat
    42001 gtcttagccg aaaaaatagc tgtgatcatc aaaactgccg ttccgggaag ttgtacgatc
    42061 acttccggta tccatgttga ccatatctcc aatgcacaaa tcaacgcctc ttttccgatg
    42121 tcagaagagt taggttacaa gattttagat tggttggaaa aaatagattt ccatatcaaa
    42181 gaaccagttt attataaaga tggtgaaaaa ccattatgaa agaagaggtt gtgaaatcag
    42241 cgttatgacc tgagtatgag agaaaaaaat ggaaaaatag cttctcatat tttgccgttt
    42301 ttgagctaat accgagggac agctgattga acgccgttta tcaagaggtt gtgataaagc
    42361 cgcttcgttt cgagtagtaa gaaaaatttt gtaaacatgg gtttccacat ttttgcataa
    42421 atttgactta ctaccgagaa actggctttt gaacaccgtt tatcaagagg ttgtgacacg
    42481 tcttttgtca caacctcttt tatttttgct tcactgtaac tgctacaata tcaccgattt
    42541 cttttccgat gtttttacga atatcttttc gtagtccaag gatgtagcag actgaaccat
    42601 ctggattttt cacgcccata ttcacgatac tgccttcata tggcacattg tcaaaagttg
    42661 cttggacttt aactcttcca gcaccgaatt cttttcttat atcatacgga aaaataatat
    42721 atgccccgcc cttgccgact tcggaagcat agatttttga ttcaaaagaa taaatttccc
    42781 tcattttatt tctcctattt tttacatcta gtatagcata gcaaaaaaaa gaaggaactg
    42841 tgacaaaagt cttgtcgcag ttcctttttt cgaataaacg gtgttctcct gccaactcct
    42901 cggtcacaag tcacaatagt caacaaacat gagaagctgt tttttgaaat tgctccttgt
    42961 gactcagagt taaacggcag gctcacaacc tcttccttaa acggtgttca aaatattatt
    43021 cgccttttgc ttctttcact aattttgcga atgcttgttg ttggaattca gcagctaagg
    43081 ctttcgtacg ttgttcttgc tctgctttgc tcatttttgg attttcagtc attgctttta
    43141 ccataccgtc ttgaacataa gcttttgctt cactttccat cgtactttgc tgtttcttca
    43201 ctaattcagc taacttggct gcttggtccg tacttaattc tacccatgtc tctgggattt
    43261 tgaatgtatt cactcggctg tcatattcgt gattgttatc agcaatattg aaccagaatt
    43321 tatacatgtc gtttttgtat tcccaataag tagttttcgt cactttttca gcatcgccgt
    43381 ctgtcttttc tactttattt gtttcgttat ccgtaccagt tgcttttggt ttttcttgtt
    43441 tttcatttgt tcggtaaaga tacacttttt cttttccatt ccctaaagct ttatacaaaa
    43501 gcatatctgc tccttgggaa tcagcactgg aaacaatttt ttctgttttt gtttccgtta
    43561 ctttttccat tccaaagtga ttggacaaat tagcgacgat caaacttagt gaagcgacga
    43621 agattagtcc gaaaatcaaa gataaaaagg tttgccatgt ttttttagcg aacacgttcg
    43681 ttaaagcaaa agcaagcaag ctgacaatca aaactccaat aatcatgcat tatcccccgc
    43741 tttcattaga acaggttctt ttttcttcaa taaaaaggca ataataaagc caagaatccc
    43801 aaagatgatc gctacgataa acgcggcatg gtaacctgac agtgtggcat tgattgcctg
    43861 atctttataa gctaatggcg tattatccaa tacagatttg cttgggatat ttgcttttgt
    43921 cacattcgtc aacacactga tcaaaatagc tgtaccaatc gagcttgcga tttgacggaa
    43981 tgtattattt accgctgtcc catgactgat caagttattt ggcaatgcat tcataccaga
    44041 agtcgttacc ggcatcatga ccatggagat cccaaacata cggatcgcat aaaggatcac
    44101 gatatataaa actggagtcg tttttgtcaa gaaagcaaat ggtgcagttg ccgctgtcaa
    44161 aatgaacatc cctgtgatgg ctaaacgttt cgcgccgtat ttatcgaaaa tcgcacccgt
    44221 cacaggcatc atagccccca tgatcaatgc acccggcaac aacatcaagc cagaatggaa
    44281 tgctgattct ccacgaatat tttgaatata caatggtacg accatttctg cccctaccat
    44341 cgccatattt gttacaccag ctaaaacagc tgcaatcgtg aatgtagatg atttgaatac
    44401 acgaagttcc aagaatggat gttccaaatg taattgacgc catgagaaca aggcgatgaa
    44461 aatcacacca acaactaaga aaccaattac tttcaagctt ccccagccgt catctcctac
    44521 actagaaaat ccgtaaagta aactaccgaa tccaatcgtt gataaaatag cggataaaat
    44581 atcaatgcta ggatttgaca atggaataac acttttcatt aggaagaatg ctaaaaccaa
    44641 gacaagaaca acgattggca gtaccatacc aaacaagtca cgccaatggt agttatcaat
    44701 gatataacca gataatgtag ggcctaaagc aggagcaagt ccaataacga tccctgacat
    44761 ccccattgct gatcctcttt tttctggcgg gaaaatagat agcatgatat tttgcaataa
    44821 tggcatcgat actcccacac cgcaagcttg gatcaaacga ccgattagta atgtactaaa
    44881 attaggtgct gtaaagcaag taatcgttcc gatcaaaaag atcgtcatgg cagaaagata
    44941 taaatttctt gaactgaatt tattgatcag ccatgctgtt attgggatca tgatcccgtt
    45001 gactagcaag aaaccagtcg ttaaccattg cacatcagac gctgaaatat cgaaagcttt
    45061 catcaatgta ggaaaagctg tcgtcaataa ggtctgattc aaaacggtac aaaatgtacc
    45121 aattaataag acaacaacta aaagtgtccg attatatggt tttccgtaaa tatctacggg
    45181 ttggttgtta ctcatatttc tccttctttc tgaatttttt ctgaaactac tagaatactt
    45241 tagtcctaaa ttcgctattt gtcaacctac ttgacacata agttaatacg gatataatta
    45301 agggaagcaa gaggacaaaa ggagtgacaa aaatggttgg ggcttccctt aaacgtttgt
    45361 tggaaaatga tcaattggtt gttggcatca gtgaattaag tgatatcgta ggtgtgtctc
    45421 cacgtcaatt aaggtattgg gaacaaaagg gattcattcg ttcgatcgaa gctgatgcga
    45481 attgtcctag aaaatatcgt ttacctacag tcataaaagt agaactgatc aaaaaatatt
    45541 tagatgacgg atttactttg tcaaaagctg cagaaaaggc agaaatccgc caaaagaaaa
    45601 tgcaccatgt ccgtaaagtt ttttcaaaag caatcaaaga tatcgaattg atcgacgaac
    45661 gctatacgat tttacaaatc ggtaacttca aagaagagaa tgctaagatg tacatcattc
    45721 atgacaacga gacagacgaa ttaacttaca agctccttcc tgtcgatgaa aaagctgact
    45781 acgaacagtt gttaaaagaa ctctaaacgc cactaatctc ctaagtacca agctttttaa
    45841 taagtagatg ctacataaat acttgaaaaa aggcctgttt gaacataaga atatccgaac
    45901 ggtgatctgc gtttctttca tatagaaagg aattctccat cactgttcgg atattttttt
    45961 atttttttcg gttactttct tctaatacat ctgttagcca atttgcatat tctgtttccc
    46021 gattgatcgc atgcgctaaa atcaggtagt gtccgtaatt ttcatgtttt tctttttcag
    46081 taggaaagat cgtttttttt cgctctaata gatgaagtcg tttttcttca tgaaggtcac
    46141 gctgctgctg aataatttct actaatgccg gatcattcac gtctttgacg aaatacaatt
    46201 tcagaacaaa ttcgtctcga ttaaccggta gttcattggt gggtgtatga atccactctg
    46261 ccaattgttc cttcccttct tctgttaatt cataaacttt cttttccaat ttttcacctg
    46321 tgatctctac ttgatgctga atgattcctt gctcttccat ttttgcaagt tcaggataaa
    46381 tctgactatg ttttgcttgc caaaattctc ctatttcatt tttgaacgat tgattcaatt
    46441 catatcctgt catcttcttc ttacttaata accctaacaa aatatatcga agtgtatttt
    46501 ttcttgccat cagttttccc tccttgacaa atctttaaat ccattctatc ataaatgagt
    46561 aatcgaaaac atgtagttta ttacatgttt taatttataa ggagatgctt aaacatgaaa
    46621 gaatttaaag aattggaaga ttttattgga actcacttta tctatacgta tgacaatggt
    46681 tgggaatatg aatggtatgc caaaaatgac catactgttg attaccgtat ccatggcgga
    46741 atggtcaaag gccgttgggt aaaagaccaa gaagctgata tcgttaaatt gacggacggc
    46801 gtattcaaaa tcacttggac agagccaaca gggacagacg tggcattgga ctttatgcca
    46861 aatgaaaaga aacttcacgg aatgatcttt ttccctaaat gggttgaaga acatccagaa
    46921 atcactgtta ctttccaaaa cgaacatatc gcagaaatgg aagcagctcg tgaaaaatat
    46981 gaaacttatc caaaattact tgttcctgaa tttgctacga tcacttatat gggtgatgca
    47041 ggtatagaca atgaagatgt tattagcgaa gaaccatacg atggaatgcc agatgatatc
    47101 cgtagcggaa aatacttcga tgaaaattat aaacgagtaa aataaggttg tgaaagagag
    47161 gttgtgaaat cagcgttctg acctgagtat tagagaaaga aacggaaaaa tagttcctca
    47221 tgttttgccg ttttttgagc taataccgag ggtcagctga ttgaacaccg tttatcaaga
    47281 ggttgtgaaa tcagcgttat gctttgagta ataagaacgc tagttagaaa atagcttttc
    47341 atattttcta ggctagtggg cttattaccg agaagcagcc gtttgaacac cgtttatcaa
    47401 gaggttgtga aatcagcgtt ttgacctgag tattagagaa acaaatggaa aaatagttcc
    47461 tcatattttg ccgttttttg agctaatacc gagggtcagc tgattgaaca ccgtttatca
    47521 aagattgtaa caactgcata ttcacacagt taaattagtg acttcaaaat gaaataatgc
    47581 tcccagcaaa aaattaggct gggagcatta tttcgttatt ctgttcatca aaaatcagcg
    47641 ttttgattcc atttcatcac aagttcctcc tcatttactg cttcatcttt ttgctgtgtt
    47701 tgaattgtaa aaccttgtga aagatagaaa tgaatagctg attgattttt ttgatagact
    47761 gtcaaagtca gtgttttatt ggtttctttt atttttttca ttagttgcgt accgattctt
    47821 tgttgccggt attccctttt cacaaagatt cctgcgataa actgatcaat caccccagca
    47881 aaaccaataa tttcttgatc tatttctgct aagtaaatca ctgcatctgg taaagctttc
    47941 ctaacagatt ctacttgatt ctcccaataa gttgatggaa tgaattcatg agaatccaag
    48001 ttagcatgca gccaaatcga tagaacagat tccagttctt tggcagacaa ttttgttgtt
    48061 gtgcggatca ttttttctct ccttacttat tcattgattt taaaataaaa accatttttt
    48121 tgaagctctt ttacaagttt tcgcccctca aaaaagtata tcttctcttg ttttactttt
    48181 ctaataccaa aagaaacagc aactttagga tcatcagctg aaacgattcg ataagttcca
    48241 ccaggcaatt gatccagctt gactttccca tgatggtcgc tttgtactga aaacggcaca
    48301 ccatttctag taagtgtttt tttcttatgt ttcaaaatga atgaactacc atttaccggt
    48361 ttccctgacg aatcactaat aataaatgaa aaatcaagca cttttttact taatctatac
    48421 tgatagatta gtcgtccgat aaacactaac agtatgacta cagctattat cgttcctgca
    48481 agaataaacc attgccgcat ggttctttcc tgatttcctt ttccttcttc tttttttacc
    48541 gattcgttat aaggaatccg atgtcctgtc actagcaaac gatgcgtgtt gatcatatac
    48601 ggcgtacatg taagcaaggt aactaagtct tctccttctg aaatcgttag aacagacgtg
    48661 tcatctggca gtaccacttg tatattttta acttgataag ccaatttctc ccccaaaaca
    48721 gtcaaaataa acgtgtctcc gttttctaaa cgatctaatt cggtaaataa ccgtcgatta
    48781 ggtaaaccgc tgtgagcaga gatcactgaa tgcgtcgaat ttccaccaag cgggaaagag
    48841 gttccttgca agacgcctgc tccattttca aggatcctac cattgagccg atcataaagt
    48901 gaaatattta attggatctt aggtatattg atacttccga tcagccattc ctctaattgg
    48961 ctggatgttg tactttcatt tctgttcttt tcattgaaag gatcagtacc aggattgaat
    49021 ccttggtcag ttaatcgttg gtttttctcc tccattttct ttcgctgcac ttcaatttca
    49081 gctgcggtcc gtttttgtac ttgttcgata cgtttttgat caataaaact atttaaagaa
    49141 tccacataaa atggatataa agcgatcagt aaccctacga cgatacatat taataaaaac
    49201 aaacgaaaca aaagtcgacg agactgtttt tgcttactct gcttcacatg tgtcctccct
    49261 tgcaagtttt tttgttcttt cttttattat atgggttcag gaaaaaaata gaaaatgaaa
    49321 gccgatgagg ttgtgaaaaa gctgtcctgc atcaagtaat aagtacagcc caaaaaaaaa
    49381 gctactcata ttttttgttg ggttgggctt attaccgcag atgcaagctt ttgaacaccg
    49441 tttaaggaag aggttgtgaa aactgcgtta tgctttgagt aataagagcg ctagtcagaa
    49501 aatagcttct catattttct aggctagtga gcttattacc gagaagcagc tgattgaaca
    49561 ccgtttaccg agaggtcatg aaaaagctac tttgtttcga gtattaagaa aaattctgtt
    49621 tcaaaacaag gagatgcaac aaaacttatg tcgcatcctc tctccttaga actttcactt
    49681 atgtttttct tgtaaaattc ataaatattt tcatgaattt tctaatttct tgcctacctc
    49741 tcgcatagga tatatgatga atttatctaa agtttgcaaa ggagttcgac aattgaaaaa
    49801 atatggccca tttttagcaa tttttttcat tgctttggca tttctcatgc ctcatttatt
    49861 cacacataaa ctaattttgg gtgctgatgc cccattccat tacaatcggt tctatgatgc
    49921 cgcgcaacaa attaaatatg gggatttcca ttattttcct tccctttatg gattccagca
    49981 aagtggcagg atcgtgaatg ccgtttacgg cccatttttc gcttatttac aaggattgat
    50041 cgtgttgctt gcaggtagct ggtttcatta tcagattctt tcaaatttca ttctttatct
    50101 tatttcagga ttttctatgc tgattttact gagatatgtc aatgttcgaa attgggtaag
    50161 tgtaccagta gccgttttat tcatgagtac gtattctatt caatactggt ctatcaacca
    50221 aggattcact agctggggga cttgttttta tcctttatgt atgatcccta ttgctgattt
    50281 cgtccttcat aaaaagttcc ctgttatcaa agtcgctgtc tcagtgggac ttatgacaca
    50341 gatccattta ctgtcttcga tcatgttagc tttgatgtat atcccttttt atctttatta
    50401 cttttttaac cagactgaaa aaggaaaagc actgcttcaa ctgattaagt cggtcttgct
    50461 ttattttgtt ctgacagcaa atatttgggc aggattagct attgtttaca gtgggaataa
    50521 gattcttcct ccctttgtta acagagaaat gagtcaaaag gcgatagatc tggatggcag
    50581 ttattggctc cattacccta aggcctttcc tatattgata ttatcaggtt ttttgctttt
    50641 tattttcttt caacaaaaga acaccttatt ccaaaaaatc gtgttgtaca caagtcttgt
    50701 tttctttttt ctttctacta gcttgattcc atggacgaca ttgatcgaaa aaaatgtgcc
    50761 ttttattagt ttgatccagt ttccatttcg atttttcgtg ccatttacgc tattgcttct
    50821 actttacctt gccctttcac tgaaccagtg gtctgaacaa aagtggttcc gtcttttaag
    50881 tatatttggc attcttattt tttcagcaca gacgatccag aacttatacc agcatctgga
    50941 aaagtgggaa aatgaaacat ttgtcagtcg acatacttat ctattcgata cgccggaaga
    51001 agcaaggaaa acattcttca gcaaagatat gtctaccagc ttgttcgttt tccaaaaaac
    51061 cacacctgac tatctgccaa tctataagga gacaaaagac aataaatacg accggtataa
    51121 cgaagaaata ctagaaaacg aaacaacttt tataaaaaca catccaggcg gtcttttgac
    51181 aatcaagtgg aaaaacactg gggacaaaac tgttcatatc cctgttatcg tctacgatcg
    51241 gactgtgcta cagcaaaatg gaaaaacatt gactgattat gaagtaacag atatcgggac
    51301 cccaatcgtg aagcagcaaa aaggaatcaa cgagctcacg cttcattatc aaacgccaat
    51361 ctatttctat ttcattcttt ctcttacttt gattggctgg tttaccttgc tatgtttatt
    51421 tatttatcat agatacaagc ttttacgtgc ttaggcaaaa gggataaata catccgatca
    51481 aaaaactctc caaacggtat aatcgtttgg agagttttat tttaatcagt ttttttatgt
    51541 tatatgttat attttttatt cgttcacatc taattcgatt tccaccccgt agtgatcact
    51601 gatgattgga ctatcgatcc catcaaaaat ccgttgatat tttttaacct gcattccttc
    51661 agggacgaaa ataaaatcta tacgcaattt ttctgtattt tgttcccaac catctatttt
    51721 cttttccaca gtagctgctt ttcctttttc attcgcgaca acatacgaat cttgaagaag
    51781 aaggcaactt tctcttacag tataataacc ttgagagtct actggattat tgaaatcacc
    51841 taggaaaaaa agtggtacgc gagtttccaa aaagtagttt tccaagtttt tccactcaaa
    51901 ataaaagcca ctttttttat ctgtccacca agaaaagtgg cagctcgaga caaaaatgtc
    51961 ctgatcatct atctttgttt tcccaatcaa gatcttgcgt gtctgataat cttctttctt
    52021 ttgaaattgg gagacggtaa ggacctgact ttccaatgtt tttttcgtaa gcagtgcatt
    52081 tccttcttca tatttatcat aaccgatgtg gctcatttcc caactccaaa aatactcttt
    52141 tccgtgttcc tttaaaagct gaactaaacg gtaggcaaaa ttatctttac ggataggtac
    52201 ttgattcttt accggacaga acttcattag cttcgcaggt tctacttctt cagattccag
    52261 taattggttg atttcttgta aagcaatcac ttcataatct tctaacagga tcttatctgc
    52321 taattcttgc aattttttct ctggttctgg ctcaagccag ctatgcgtgt tgagtgtcat
    52381 gaatttcatt ggttttctcc ttgtcttcat ttcgaaacaa aagggttgtg acaaaatttt
    52441 tgtcacaacc tctccagcaa atagtgttct cctgctaact cctcagtaat aagctcaatg
    52501 gaccaggagc tattttttgt ttggctgttt ttatcacttg gtgcagggca gctttttcac
    52561 gacctctcca ctaaacagta ttcaaactgc tgcttctcgg taataagccc aaccaaacga
    52621 aaaataggag gagctatttt tgtttggctg ttcttatgac ttgatgcagg acagcttttt
    52681 cacaacctct ccttacattt taatggagcc gatcacttta ccagctgtca ctgtacctaa
    52741 ttgcttgatc actacttctt ctactttatc gtttgtaaat acgatcatga tcgttggatc
    52801 tttttcttcg ttttggatca catcaagatc catctcagct aatttcgttc ctgttcctac
    52861 ttcttggcct tctttgacag caatctcaaa agctggctga ttcatttgga ctgtatcgat
    52921 tcccatatgg atcaaaactt cgataccatc tttcgtttcc agcccaattg catgtttgct
    52981 agggaaaata cttgtcactt ttccagcgat cggcgcaaca acttctcttg atgttggaat
    53041 aacagcaaag ccgtcaccca tcattttttg tgagaatacc ggatctttta cttcagataa
    53101 aggaatcact tctccagaag ctgcggaaac tagttcttgt tctataccta agtattgctt
    53161 actacctgct tgtccatttc ctgtagaaac catttctggc tctggaatct ctgctcctga
    53221 ttgcagcaga tcttcgatat ctgatttcaa gacatctgct tttggtccat aaactgcttg
    53281 gactccattg tctttgacga tcaatcccat cgcccctgct cgtttccagg cttcttcagt
    53341 ccctactttt tctcgatcat ttacacttac gcgtaaccga gtcatacaag catcgacatc
    53401 tttgatattt tcttttcctc ccagcaaatg aatgatctgg accacttgct gatctgctgt
    53461 tcctgttccg ctgttagata caacggtttc ttcactgtca ttgtcgtaat ttccgttacg
    53521 tcccggtgta gcaaagttga attttttgat caagaaattt gctaagaaat acgtaacaac
    53581 accaaaagcg atcacacaca acacgaagtt gataagatct ccacctagac ccgctttgat
    53641 agctaacggt gtccgagtca gcaattcgat attcccaaat gaatggaccc gcaatggcaa
    53701 gatatctgcc atcgcaaatg cagccccttg gatcactgaa taaatgacat ataatggcac
    53761 agcagcaaac ataaacatga actccaatgg ttctgttaca cctgttagaa agacagccaa
    53821 cgctgctgaa aaatacattg atttatatct tgctttctta tcaggatcga cattacgata
    53881 catcgcaaaa gccattccca tcaagatacc tgaagaacca atcatttgtc ctactttgaa
    53941 acgagccggc gtccagttat gcaacacaaa ctcatattgc gacatatccc ctgcaccttt
    54001 aagattcact aaatcagtgg cccaagccaa ccataacgga tcttgcccaa atacttgtgt
    54061 ccccgcctgt gcgcctgata aaatctcata cgatccgcca agctgtgtat agttgatagg
    54121 aatcgtcagc atatgatgca atccaaatgg caatagtaaa cgttctaatg ttccaaataa
    54181 aaacggtgct agaatcggtg cgctttcttg tgattctgca atccataaac caaaattatt
    54241 gattccagcc tgtacgtttg gccacaccac agcaagaatc aatgccacga ttgttgacca
    54301 taagataaca acaaacggaa caaagcgctt tccattgaaa aatgacaaag catccggcaa
    54361 ttttctataa ttatagtact tattataagc cattgccccg acaaatccag cgatgatccc
    54421 tacgaatact cccatattca aagctggtgc ttccaataca cttgtaaaaa atcctttaac
    54481 catgatcttc gtaccgaaca gagtgtgggt aaatgcatct tcattcgcaa gcatgtcact
    54541 tgtgacacca aagatagccc cagtgatccg attgattaaa ataaatgaga tcccagctgc
    54601 aaatgctccg cctgctcttt cttttgccca acttcctcct attgctaaag caaataaaat
    54661 atgaagatta ccaataattg cccaaccaat attttcaatt actccgcccg ttgtgacaag
    54721 cggcgtccaa tcaggattga tcaagggaat tgatttccct atactgatca tcaacccagc
    54781 tgccggcata actgctacca caaccattaa agcctttcca aatttttgcc aaaactcaaa
    54841 actaaatatc tttttcattc cgtttagctc ctttacgcaa tcggtttcgt taaacgtatt
    54901 atagcgtgcg ttttcttttt tgtaaatgct ttatttttta tttttctgtc tataccattc
    54961 tataaaacct ataatacgtg tgttcccttc tataattttc tcctaaaaat ttattttttt
    55021 aagctttttt ctatgaaata ttttcttttg tctattgact tgtgcaatcg gttgcgttta
    55081 ttatgaacgt ataaaacagc tcattaggag gagaaaaatg aaacagatca aacgactatt
    55141 tgctattgat ccatggaaaa tcagcacgac acaattagat aaaaaaaatc ttcgactgca
    55201 agaatcttta acaagtattg gaaacggtta tatggggatg cgcgggaact ttgaagaaac
    55261 ttactcaggg gaccaccatc aaggtactta tttggcgggt gtctggtatc ccgataagac
    55321 acgtgtcggc tggtggaaaa atggttatcc agaatacttt ggaaaagtga tcaacgcctt
    55381 gaactttatc gcgatagata tttcgatcaa cgatcagctg atcgacttgg cttctttgga
    55441 accagaagac ttttactggg aattagatat gaaaaatggt gtattatctc gtacattcac
    55501 aatcactact tctactaata aagtacgcat ttccttcgaa cgttttctga gcattgtcaa
    55561 aaaagaatca gcttatatct atttaactat agaaatgctt gaaggtagcg gaacagtcaa
    55621 actcgtatca aaattggatg gaaatgttca aaacgaagac agcaactatg atgaacattt
    55681 ttggaatgag attgatcgtg gaactataga agaaatcggc tatttgacga ctaaaactat
    55741 tccaaacaac tttgggattg aacaatttac tgttactgca gcaatgcgcc atttcgttaa
    55801 tggaaaatca gtcatccctg actatcacga agatacttta gcattacatg gttgcttgtc
    55861 tgcagaatta tctcaaggtg agacatggtc agtggaaaaa gaaattatcg ttcttactag
    55921 tagagatatt ccagaagcgc agcaagcaga aaaagctgtc tcagaattaa cgaaccagac
    55981 aaacaattat atccaagcaa aagaagagca aacagcggct tgggcagaac gatggaagct
    56041 tgccgatgtg gtcatcgaag gagacgaaga agcacaacaa ggtattcgat tcaatttgtt
    56101 ccaactattc tctacttact acggcgaaga tgaacgattg aatatcggcc ccaaaggatt
    56161 cacaggagaa aaatacggcg gagccactta ctgggataca gaagcgtatg ctgtccctct
    56221 ttatcttgct ttagccaaac ctgaagtaac taagaatctg ctgaaatatc ggcataatca
    56281 actgccgcaa gcaatccaca acgctcagca acaaggatta aaaggcgcgc tttatcctat
    56341 ggtcaccttc actggtgtcg aatgccataa cgagtgggaa atcacattcg aggagattca
    56401 ccgaaatggc gcgattgcct atgcaatcta caattatacg aattacaccg gcgatacgga
    56461 atatttgaaa aatgaaggac tggaagtttt agcagaaatc gcacgcttct gggcagaccg
    56521 tgtccatttt tcaaaacgag caaataaata catgatccac ggtgtgaccg gcccaaacga
    56581 atatgagaac aatatcaata acaattggta tacaaatacg atcgcagctt gggtcttgcg
    56641 ttacacgcga gaaaattatt taacttatca aaaagaagct gctatcacta tcagcgaaga
    56701 agaactagcc aaatggaaag atatcgcaga gaacatgtac ttcccagttg acgaagaatt
    56761 aggaatcttt gtccagcatg acacattctt agataaagat ctcatgcctg tatccgatat
    56821 tcccacaagc gatcttcctc tgaatcagca ttggtcttgg gacaaaatcc ttagatcttg
    56881 cttcatcaag caggcagatg tactacaggg gatttatttc ttcaatgata cttttacaaa
    56941 agaagaaaag caaaaaaact ttgatttcta tgaaccaatg actgtccatg agtcttccct
    57001 ttcgccaagt atccattcga ttttagcagc agaattagga atggaaagca aagctgtaga
    57061 aatgtaccag cgaacagctc gcttggattt ggataattac aataacgaca cagaagacgg
    57121 cttgcacatc acttccatga ctggtagctg gttgtcgatt gtccaaggat ttgctcaaat
    57181 gaaaactgcc catgatcagt tgagattcgc accatttctg ccaaaaactt ggaaagctta
    57241 ttctttccat atcaattatc gcggacgttt attgttcatc tcagtcacag atgaacaagt
    57301 tactttcgag ttattagaag gttctcctct agaaatttac gtctatgaac agtcttattc
    57361 tttagcggat cgtctagaaa taacgctaag aaaggaggaa gcaaatgttt aaaggggtat
    57421 tgtttgattt agacggtgtc attaccgata ctgctgaatt ccattatcgt gcttggcgaa
    57481 agcttggtca ggaaatcggc attacgatcg accacacatt caacgaacag ctaaaaggcg
    57541 tcagccgaga agactcactc tctttgcttt tagcttatgg cggaaaagaa cactcttttt
    57601 caaaggaaga atttgcagaa ttagcgaaaa gaaaaaacga ttattattta gagatgatcc
    57661 agacgatcga accaaaagat gtcttccccg gggtcgtgcc tttactggat tctttaaaag
    57721 aagaaaaaat caagattgct cttgcttctg caagtaaaaa cgggccattc ctacttgaaa
    57781 aaatgggtct tacaccttac tttgatgcta ttgccgaccc ggcagaagca acaaacggaa
    57841 aacctgcgcc agatatcttc ctccttgcag caaaagctgt cggactaaaa gcagaagaat
    57901 gtatcgggat cgaagacgcg caagccggta tccaagccat cctctcaagt ggcgctcaac
    57961 caattggcgt gggacgtgct gaagaccttg gagaggagat cccgcttgtc cctgatacga
    58021 cttttcttac gatcgattac ttgaaagaaa agtggcatga ccatggataa tccagagatc
    58081 tccgtcgtac cagttccaga gactgaaata aagaaaatca cattgaagaa cgactactta
    58141 gaagtcgttc ttctcaatta cggtgcccgc ctgcaccaaa tcttcgcacc agacaaagaa
    58201 ggaaaatcag aaaatgtttt attgtcttac gatcgtttcg aagatgttct aacagacaaa
    58261 agcttttttg gagcaactgt cggacctgta gccggaagga tccgggatgc ttcttggggt
    58321 gagcatagat tggagaaaaa ctgcggatca caccatatcc acggtggcac gaaaggctgg
    58381 tctttccaat tttgggaagt agaagttttc aaaactccat catcaatagg tgtggtcttt
    58441 tacttaaaag atgaattttc tacctatcca ggaccaatca ctgctacgat cacttaccgt
    58501 ttaacaaaaa acgaattaga gatgacaacc tctgctagta gccttgttga aaccatctgc
    58561 aatccgacca accatgccta cttcaacctt tcaggaaatg gaacaagaga catctatgaa
    58621 catcagttaa cggtcttttt agatggtgtg cttgaattag atcaagaaaa actgcctact
    58681 ggaaattgga caaaaaaaga aaaactgcca atcgacttta gaaaatcccc aacactgcaa
    58741 gagatccttg catgttatcc agatggacta gatgatgtat ttcttttgca tcatcctagg
    58801 ctttccaaaa cttccttaca attattcgag aagcattctg gtcgccaaat gactatagca
    58861 acttctaata aaagtatggt attattttct acgactggat tcgaagctga tttctctgtc
    58921 aatggaaaac agatgcattc aaactacgga ttagcaatcg aaccgcaaga aatccctgat
    58981 atcgttcatt tccccaaatt tggatcgatc aacctccatc ctggtcaaga acggatcagt
    59041 cagacgattt atcggttctc tgcacaataa caagagaaag gtgtttttat catggctatt
    59101 accgtaaaag atgtggcgaa aaaagccggt gttgccacct cgaccgtctc tcgtgtcatc
    59161 aacgatcatc ctagtatttc cgagacaaca aagaagaaag tccgaaaaat tatggatgaa
    59221 ctaggttatg ttcctaacat gactgcccgc aatctaggaa aacgcatttc cagtgccatc
    59281 ggcgtaatct taccccctct tgattcaaaa gagcggatcg gtaacccatt ttatcttgag
    59341 atcatggaag caatcaacga agaagcgcgg aattatgata tgactacagc aatcgctact
    59401 gctaaaaatt ttgaattgct gttagagaac gtccagcgta tgcatttaca aaaacaagta
    59461 gatggtttta ttctagtgta ttcagatagt caagatactg tcattgatta tttataccaa
    59521 aaccacatcc ctttcacttt gatcggacag ccttatcatc atgagaacga aatcgtttat
    59581 gtagataatg ataaccagct tctaggtaaa caagcaacag aatttttgat ccagaatgga
    59641 cataagaata tcctgtttgt gacaaacacg actcatgaaa gtttgtattt cgaacgatat
    59701 ttcggttatc aaaaagcaat gatgatggct ggtcttcctg cgttatccag tgtagtacta
    59761 gagcatccgg aggattatct gtcctttgag aaaacgttga aagaaaacga agccacagca
    59821 ttggtggtca ttgacgatat cttcgccctt cgcacgatgc agttggcgca aatgtatggc
    59881 tatgtcattc cagagacact ctccgtcatt agcttcaaca actcgatctt ctcaacatta
    59941 acacaccctt accttacaag tatcgatatc gatatctccg aattaggacg tgtcgctatg
    60001 caaaagctgc gcgaattgat ccatgaagaa ttgagcagtg gcgtacgttt ggtcatccct
    60061 catcgcttga tcaaaagaga gacagtcgtt cctttagaaa acccatacta aatagctgac
    60121 tcactaaata cgctgaccat ttatcctatg gtcagctttt tatgtaaaag aagatactac
    60181 ttctaacaca ttgtttatct gtaaaaaaga gcgtgggaca aaagtaaaga acacttttgt
    60241 ctcacgctct aaacacgtat aaacggcgga agcagaagca actccttcgg aaataagctg
    60301 aaattcacaa aaatttaaaa agcaattttc ttgagtttct tcttatttct tggagttaaa
    60361 cgcttctgtc ccagcctctt ttttccttcc ttcaattttt tacacgtcga cgattcaata
    60421 aaaaaccgcc taatattgta aagacaggtg caagaatcga gaaaaaggcg aatggcaagt
    60481 aatgcaacgc gctaactttc atcgttccca taatgaatgt gccgctgact ccccacggta
    60541 cgagagaatt gacggtcgca cctgaatccg ctagtgttcg tgtcaaatag gtttttgata
    60601 agccaacgtc atcaaaagaa cgcttgaatg tttctcctgg aagaatgatg gacagatact
    60661 gttctccaac gatcagattg attccgatac agctcaaggc agtaaacccg actaaacgag
    60721 aaggtcggtc catcttttct tttaattctt gaacgatggt atcaacgatc agatatttga
    60781 tcagtaaccc acctaatgcc aatgcaagta aaatcaaact agcagaacct aacatactca
    60841 tgatcccgcc tcgagaaagc agttcatcta gctttggatc aggtgtattt gcgacataac
    60901 cattcatcaa gatgtctgct gcttttccga ccgtcaaact tttctcatgg ataaaagaaa
    60961 gaacgagagc agctgtcgat cccgctagta atgtagggat agccggaatt tttttgattg
    61021 cccccccaaa taaaataact agtggaatca atgtccaagg agagatccaa aagctcgctt
    61081 tcatgttttg gatcatttca ttgatactag ctagatcggc tgaatgactg ttatgtccta
    61141 atatcgtata aaaaatcaac gaaaggacga atgctgggaa atccgtataa cgcataccag
    61201 cgatatgttc gaatagatta actcctccaa tacccgctgc caagttggtc gtatctgata
    61261 aaggcgagca gttattccct aaaaaggcac cggaaacgac tgcgcctgta gtcattgcat
    61321 cattgaatcc aagtaaatga ccgatcccca aaaaagcaat tccaatcgtc gaaatcgttg
    61381 taaatgaact gccgacagtc actccgacaa ttgcacaaac aagaaatgct gtcggtaaaa
    61441 agaaacgcac agaaacaagt ttaaatccat aaaccataat cgtagggatg gttccagata
    61501 aaatccatga agcaactaag acaccaatca aaagaaaaat caggattgga atgatccctg
    61561 gagtgattcc tttgatgatc ccgtcatgga tctcatccca tgtgaacttt ttaaatctgc
    61621 cataaaacaa caaacacatg aaagctgcaa ggatcgggat atgtggcgag agctggaatc
    61681 cgatgatcag tactcctagg agtgccaaca acacgatcaa tacagcgata ctttctttta
    61741 aagaaatatt tttattcatc gtcgatctcc tatttcaaat gaaaaggccc tgccatgaaa
    61801 aaactattca cttggcagga cctcctacta aatcagcgac aaaacgtcgg ctgaaagact
    61861 tattttttta tttttcttct aaactttttt ctacctcaat cccccaagaa tctttcttga
    61921 agtgattgat gatcaatgga ataacgaaca tgaccactgt gatagctgct actaataatg
    61981 catcactcaa tgtcgctgta cctaatgtag caaatacgac aacgatgata gagaagatca
    62041 gcatgcagct gactacccaa gctaatccgt ttccgttttt accaattcgg taaggacgtt
    62101 cgaattctgg atgtttctta cgcattttaa gcaatgaaac agcaatcaaa atgtacacga
    62161 tacaataaat aacagttgta gcatttgtaa gtgtcaagaa tacagcactt acgtcatcca
    62221 ttacaccata aagcaaagca aataatgaaa taacgattga ttgtgttaga acgacatttc
    62281 tagatacgcc gtatttgttt tctttatgga atccgaattt tggtggaagg aatccttcac
    62341 gagctacttg gatgattgtt tttgatggtc cagtaaccca agctgataat tgtaaaagca
    62401 caccaataaa taccatgaag ctgaagatat ttccgatcac ttctggtaag cctaggattt
    62461 gacagtcaat caagataggc tgcgtgatat ttgctaattc catttttcca tctggtacga
    62521 catttgctac aagcatcgca ttgatgacat tcaataaaac taatccgatc aaagcgataa
    62581 atacgccttt tgtataattt tttgttgcgt ctttcaaacg aggcatgtaa acagaactca
    62641 tttcgatccc aacaaaaata aatgtgatac cagctaagta tttcaatgaa tcaatatgtt
    62701 ccaagtcagg gaaagctttt gaccatgaaa agtcacctag atagccgttt gagactagtc
    62761 cgactttgaa ggctgccaaa acacctaaaa cgaacatgat gactactggg atatataccc
    62821 ctagccatac accgatgttc ccgccgactt ttgccatatc gaatttcaag ttcaagatcg
    62881 tgatgatcca gtagataact aaaatacaac ctaaaacgaa ccagtgatta ttgccaagtt
    62941 ctacattacc aaatgtgttt cctaacaatg gtcctaaagt agaagcgacc attaccatac
    63001 ctgggaacat ttgcacccat aatagccatg caacgacaaa tccccatttg ctgcccagtg
    63061 ctgtttttac ccataactga ggaccgcctt cttgaggaag catcgtggaa agctcaccgg
    63121 aaaccattga tatcggaccg gcaaaaaaca aaactgcaaa aattgaataa aaaatcaatg
    63181 tccaaccgac tgcggctaac gtaggaatac tccgaaccgt tgcacacagt gccattgtca
    63241 gaccgataaa ggtcccaaga cccattttac ttacttcaga tttactcacc gttgacactc
    63301 tccttgattt tttggataaa ataacggtgt ctttgaagta gtttcagcaa taaacgaaac
    63361 aattcagcga atatttgagg ggattctttg agtttattgc ttgaagggtt tcttcaaaga
    63421 caacctcaaa acaaagggat cgaagagctt aggtctcccc ttgctcttca gatccaaaca
    63481 tgcgtcagaa acagttagta ttcaagctgt tttctaacat gattaccttg cttattttgc
    63541 ttcgcttgcc aataattggt ctgcgtagat tttttccaat ttttcttgaa gagctgcttt
    63601 gattttttca gcatattctt caaagtgttc ttctttgttc atgtatggtg tcattacgct
    63661 ggcacgcaat acagttactt ttccggcacg gttccattct tcatctgaga atcctagttg
    63721 gttcacaaat tgtaatgggc tgttgccata gtctgggata gcaaagtcag tatgtgaagt
    63781 taagaactca ttaccgtaaa tgcttccttt cacgtatgaa gaataatcat atacgtcatg
    63841 gttcaattta ttcattgcaa ctaagtcatc gttgcctttt tctttgaata catagtctac
    63901 catattgaaa tctggataag ttaatggatg aacttcgatt tctttgtctc ctactttaaa
    63961 tgacaagtca ttcaagaagt tgtagaaacg atgtgcacct tcgattgatg cacccattaa
    64021 tttaccatat cctgtgacat tcaaaggtaa gacatggtgt gctgcccaaa cgctggctgc
    64081 tgttgcccct gcttttgaac cttccaagat ataagcccca agtaatgctg ggatatctgc
    64141 gcctttttca aatacatatg tcgcaaagta agagataacg tctctcatgc ggatatcttt
    64201 gatgacgata ccaccggcag aatatggaac atagcccatt ttatgtgggt caatcgttac
    64261 agattctgct tcttcgattg ctttatatgc actgtgtact tcttctaaga tataatcttt
    64321 gttttctgta aagacattgt atttgtaatg aacatctttt aaatcttcaa atggaatgaa
    64381 gttgttgtct tcgtctaaga aaatcgcacg gccgtatccg ccataagcag catctacgtg
    64441 taagtagaag taaataccgt ctttttctaa tacgcgacgc aatgcaacga ttttatcgat
    64501 accgtcgatt gctccttctt ctgttgatcc tacaacaccg actacaccta ggattggtgt
    64561 tttttcagct gctagaccac gaacgatttt ttcaagttcg ttgatgtcca tgcgatagtt
    64621 gtggtcaact ggcactggaa tcacttggtc taaaccgata ccgatgatgt cggcagcttt
    64681 tagccatgaa tagtgtttag tttgtggaac caaccatttt cctaattttt ctaaatgttt
    64741 tccgctacgt gctgagtgag ctttgatttc gtcgattttt tctgggacgc tgtctaacag
    64801 attcatgatt tcttctgttg acaagttcat taattcccaa tcgctttttc cagcaactag
    64861 ttcaggtgtc acttctttca ttgcaagcgg caatgatttg atgttgcggg cataccaaag
    64921 tccttctaag tttgctaaag aaccatcagc aacgatgtgg ccccagccat ctttatagct
    64981 cattaatttt gcaaattcca ttcctacttc ttcttccatt tggcttgttg caggagaaga
    65041 ttcataggca acgttattac cattccatag catagcaaaa ttgtacgcta gtaaagatgg
    65101 catcaatgtt tcagagttca tatgacccca gtaacgtccg gcattatgcc aaggaacgga
    65161 atgggtacgc atccgcgctg aaatttctga tagaacatcc tttgttttat tcacagtgtg
    65221 ttcaaaacta gcactgcttt tttcttctgg cgtaataata ggcatgtctt gaggcatgta
    65281 gttttgacgc cagcctaaat gttcatccac caattcattc aacaaagctt tatagatttg
    65341 gccgttctca gctttgtccc caatgaatag ggcatttagg tttagatctt tcgacaatga
    65401 ttcactcata gtgaaaccct cctaaaaaaa tatttaaatt ttgtcgagcc ctacattaca
    65461 cctgttcttc tattctgtaa actaagtttt ggcctgattt ttaatcatat tttaatatgt
    65521 atcaattatc cgaattgttc acacttttga aataattaaa aaaaacgagg gaatttacaa
    65581 atgtaaactc cctcgcaaag attgtattaa cgacaaccag cgttctattt taggatgggt
    65641 ttcaaaattg gaatgttgta atagtatttc cctttttcaa ggtaaaaact tactaaattc
    65701 taatcaatta ctttggccaa aaagtaattt ttcttacctt ttcgaataac cacaaactta
    65761 ccatcgaatt catcggatgg atttatgaca aaatttaaat cggtaacgcg gtcaccgtta
    65821 atacttatag ctccgtttga aacatcttca cgtgcttgac gtttagatgg ttcaattttt
    65881 gtggaaacaa gcagttccac gatattttca ggtgtgcttg aaatttcaac gttcggcatt
    65941 ttaccgaatc cttgcgcgat ttcttccgca tttagttctt taatatttcc agagaacaat
    66001 gcttgcgtta ttttttgcgc ttctttcaag tcttcttcac tatgcacaaa tcttgtaact
    66061 tcttctgcca aacggcgttg tgcttcacgt ttctcaggtt ctgtctctac ttttttcgct
    66121 aagtcttcga tttcttcttg agacaagaaa gtaaagaatt tcaagtattt cactacatcg
    66181 cgatcatctt gattcaacca gaattggtag aactcaaatg gtgaagtttt cttcggatca
    66241 agccaaacgg caccgcctgc agtttttccg aatttcgttc cgtcagcctt cagcatcaat
    66301 gggatcgtta aaccgaacgc ttctgcttct ggtccttcta attttcggat taggtctaat
    66361 ccagctgtga tatttcccca ttggtctgct ccaccgattt gcaattgcac atcgtatgtt
    66421 ttatgcagat gcaaaaagtc gatagattgc aggatttggt aagtaaactc tgtaaatgag
    66481 attcctactt ccaaacggct ggcaacgata tcttttgcta acatcgtgtt gatgttgaag
    66541 tttttaccgt agtctctcaa aaattcaagt aaagagattt ttgacaacca gtcgtagttg
    66601 ttcacaaatg tgatatttgc atctttacca aacaattttc tcatttgatt ggaaagtgcg
    66661 tccacgttat gttgcacttg ttccatcgtt tgcaacacac gttctgtttt gcggccgctt
    66721 gggtcgccga tcgttcctgt tccgccgcca atcagaatat atggatgatg gcctgccaat
    66781 tggaatcttt tcatcatcat aaatggaatc aaatgtccga tatgcatcga atcaccagtt
    66841 ggatcaacgc cgcaataaag tgaaatgctc ttttcttcaa cgagctcgcg aagtctttct
    66901 tcgtttgttt gctgattaat ggcgtcgcgc caagttaatt catcaataat attcatttta
    66961 ctgacctccg tgtagttttt ttatgaaagc aggaacctgc tagcatgtgt aattaattat
    67021 ttcttcagga taggatagga aataaaaaaa gtcccttggc atataagcca agggacgaaa
    67081 tctcgcggta ccacccaatt tgtatgtcta gatatccaac atacctctcg aacatttaac
    67141 gctgtcgcgt ggcaatcact gccaaagact cctgaaatgt aattcgcttt aaagcatgta
    67201 ctggtttgca gcgaccacca gctttctgaa acattggctt taacaactac ttcgtttcga
    67261 tcttcgtctt gttattatta atatgtgtct atcatagagc gcaacttttt ctttgtcaac
    67321 agaaaaagga tatttttttt aaattcctca ttttttcttt tttgaatccg ctctaattcc
    67381 gaacatttta ttttcaatta ttgttaaaag aaataacttt ttctgtattt tttctgcaat
    67441 ttatcgagaa gtcactttac tttgcctctt attctttctt cgttacaata aggaatggga
    67501 caagtaaaaa aatgaaaaag caagggacga ggtatatgga aaaaaagcaa aaaattattt
    67561 taggagccac ttctgcttta actatcgtta ttttaattct aagtttgttt tttgtcatcc
    67621 gatcatctac aaaaaaagaa ccaatcattc ctgaaaaaaa aataacagaa aaagcaaaag
    67681 aaccgacaga tgataagcaa caactagtta ctcaagcaaa aaatctggct caaagctacg
    67741 attatgacca agcgattgcg ttattggaaa aagatagtaa gaacggatct actgaaacac
    67801 aacagcttct tcaaacacta aaaaaggaga aagaacaact gatcgactgg tctgatccta
    67861 ctcagatctc tcatgtcttt ttccacagct tgatcgtgga cccgcaaaaa gccttccact
    67921 ctcaacaggc acaaggatac aaagattata tggtgactgt tgaagaattc aaccgttcga
    67981 tcgaacaact ttatcaaaat gattttgttt tagttaattt gaatgatttg atccaaaaag
    68041 acgaacaagg aaatctttcc tttaacgggc ttgcgctacc tgaaggaaaa aaaccattgg
    68101 ttctttcaca agatgatgtc agctattatg aatatatgga taattccggt tttcctagcc
    68161 gattggtcgt atcaaataac aatgaaatca aaaatgtcta taaagagaaa gataaagaag
    68221 aaatcggtga ctttgacatg gttcctctga tcgatgcctt tgtcaagaaa catcctgatt
    68281 tttcttatcg aggcgcaaaa ggtaccttgg ccctgacagg ttacaacgga gttttaggtt
    68341 atcgaacatc aaaatctcaa tatggagaca atgaaaaaac aaaaaaagag attaaagaag
    68401 ctaaaaaagt ggcggaacag ttgaagaaag acggctggag ctttgcttcc catacatggg
    68461 gtcacttgaa tatgactcaa gcttcactag aagatgtaaa aaaagacaat gaattgtggc
    68521 agcaagaagt tgcacctatc ctaggaaaaa caaatattct gatttatcca tttggtgcag
    68581 acatcagcga ctggcagcct tattcagcag aaaatcaaaa atttgcttac ttgaagagtc
    68641 aaggtttcga tattttctgt aatgtcgatg cttctacacc agcttgggga caattaggta
    68701 cggatttcta tcgaaatgct cggatcaata tcgatggcat tcgttttcaa tctgatttag
    68761 acggaaaaaa tcctattctt gatccattca tcaatgtcca tgatgtctat gacaaagaag
    68821 cacgcgaatc tgcttgattt tacgtcattt atgaatcgag tagaggggaa agccaactga
    68881 ccttccgccc ctctcacacc accgtacgta cggacccgta tacggcggtt caataagttg
    68941 agtgtacgaa ctggtacttc aaggagatgt cctcatatcc ttggcgggcc agttctttgt
    69001 ctgttaatga tcgatgaaga atatgacttt tggagactga ccaaatacct tttcttgtat
    69061 ttgaccattc atacgctttc gctttatcga tccctaaacg aatcagattt tttcttttgg
    69121 ttcttggatt cttccattgt ttccaaatat attgtctgat tcttcgcttc aaccaaccgt
    69181 ttaactccct catgaattct ttcatctcac caattccata gtagttgatc caaccagtca
    69241 ttagttgctt gatttctttt aaaataagtt ggatacttct tccacggttt cttttagtga
    69301 gttgtttgag cttgcttttc acacgctgtt ttgcttttgc gtgagggcga attttaatac
    69361 ctttctttgt tggcagaatg cagaatccaa gaaactttcg tttcaatgga cttcctaccg
    69421 cactcttttc tcgatttacc gtcaacttta gttgttcttc gagaaattga atggatttct
    69481 tcatcacacg ctgtcctgct cgtttgcttc taacatagat attgcagtca tcggcgtatc
    69541 gaacaaattt atgtcctaga ttttctagca cttgatcaaa ttcgttcagg tagatgttac
    69601 ttaacagggg agagagattg cctccttggg gcgttccttc ttcagttgtt gtgatgagtc
    69661 cattttccat tacaccactc attaagtatt gacggatcag ttttaggact tgtttgtcct
    69721 ttactttccg ctcaatgaaa tacatcagct tatcgtgatt tacggtgtca aagtaggctt
    69781 tcatatcaat gtccaccaca tatcggtaac cttgttcata ataagatttt gctttcttca
    69841 ctgcgtcatg tgcgctgcga tttgggcgaa acccataact attatctgag aattctggtt
    69901 caaatagcgg ttgtaacact tgattgattg cctgttgaag catacgatca ataaccgtgg
    69961 gaattcctag ttttcttaca ccgccgtttg gtttcggtat ttctactcgc aaaacaggct
    70021 gaggtttata gttcccttgg cgtaaattgg atagtaattc ctcgcggtgc tctttcaagt
    70081 aaggaagcag ttgatcgacg gtcatcccat ctgcaccagc cgccccttta tttctgacta
    70141 cttgaagata cgcttgattg agattattcc gatctagaat tctcccaaac agattttcta
    70201 caccatcttg tttcctctgt tcaccgcaag aagtgctgcg cacttcatca tacccttcgt
    70261 gttccacact agctctttga tgatagccaa gttttcttgt tttctgcggt cgtcgcatct
    70321 tctcacacct cctaaattat tcaagattct tattgttcag tccttcgtaa cggtcagtgt
    70381 cgttactact atgacctcgg ctgacttctc atgattcacc atcatgtcgc catgatcgtt
    70441 gtgctggttt gttttcattc aacgcccaca acaccctcat gagatctccc ccggtaagag
    70501 tgaaaacttt cctcccatgt aactgctaga tttactgtat aggtttcggg cagtattgga
    70561 ctttgtcttg tgatgaagac ttatccgacc taattcagcc ttgtatctag tttctgttcg
    70621 tcagttcagg agtttgcgtc cgacttcctt cagccacaac ctcacgattg ccgccttgtc
    70681 tttcgctaac agttcctact gccaagcctg tagtggactt tcaccaccaa gttttcaccc
    70741 atgcagggcg cacaccaaaa aagccgaact gacattttat cgtcagtttc ggctttttta
    70801 tgctttattt cttcttcggt gcgattccct tgatcaggac ttctgtccat tctgctgtat
    70861 atggaagcag ttttgcgtat atctccggct tagaaattcc ttctaccatt gcttgaaaat
    70921 agaggatcaa gaactcatcc gaatatttca aatccacttt tccttctttc cgccctcgat
    70981 ggaaaagatc aagcatgata ctgaaacttt cttgcgtata cgcatccatc gcgtcagaaa
    71041 attctttttt ttcttttttt gtatagtatg tcatcaaatc gatataaaat tgtttgttca
    71101 ctcgattgag ataacggatt ttgttttcac tcatggcaat cagtgtttct tcaaatggaa
    71161 gattttccgc aatcaacgct ttcgcttctt gccctaaatc attcataaat gcaaaaaata
    71221 cttcataaat cagcccttcc ttgctgccaa aatatttaaa aatcgtcgct ttacttacct
    71281 gagcgttctc agctacctta tccattgtca ctccagcggt tcctatgtct gtgttcatca
    71341 agtcaaaagc tgcttgtaaa atctctttct ttttctcttg tgctctcttt tcaaaaccgt
    71401 tcatttgtag agcctcccgt aattttaaac ttttataata tatatagttc aaatttattc
    71461 tgaggtttat tataactatc tattgagaaa gtatcaagct agcaattcct tgtttatgaa
    71521 ttcattgaca gaaatttata aaaagaatat tatgtactaa aataataaaa aaagttttat
    71581 aaaaaggtat aaaacctttt tattaaacta tttaatcttg tttagtatat atttttagaa
    71641 aaaggagtgg ggtttttatg gcagaaatcg tcaaagtcac acatttgaaa aagagctttg
    71701 gtcgtttcga agctttaaaa gatgtctctt tcaccgtaca ttcaggtgaa gtcgttggat
    71761 ttatcggacc aaatggttct ggaaaatcca cgacgattcg gacactttta gggattataa
    71821 aaaaagacgg cggaacggca acgatttttg ggcaagatgt atgggaagat agtttatcta
    71881 tccatgaacg tctttcttat gttccaggcg atatctcttt gtggggaaat ttgactggag
    71941 gagcgatcat cgatctcttc atgaagcttc atggaaaagg gagtgctcaa aaaaaagatg
    72001 aactgattca acgtttctct cttgatccta agaaaaaagc aaaaaactat tcaaaaggaa
    72061 atcgccaaaa agtgggcttg attgctgctt tggctgttga ttcggatctc tatttatttg
    72121 atgagcctac ttctggttta gatcctttga tggaaacgat tttccaagaa gaagttgaaa
    72181 aattgaaaga acagggcaaa tccatcctac tttcttctca tattctcagt gaagtcgagc
    72241 gactagcgga taaagtcgtg atcatacgcg aaggaacact cgatgaagaa ggaacactcg
    72301 atgaattgcg tcatttgact cgttcgacca tcactataga aacagaagca gagaatagtc
    72361 acttagcaca attgaacggt gtccatgact ttgtgcaaaa aggtcacctc gctcaatttg
    72421 ctgctgataa cgaagctttc gatgtgattt tagctgaagc gtccaaatta ggtgtgaaaa
    72481 aatttgaatc tattccaccg actcttgagg atttattcat gagacattat gaaggatgag
    72541 gaggaagcga aatgactgat aaattttcac gctggtccac ccttttttct caaaacctta
    72601 gccgtgactg gaaaaagatc ctgatatggc tagtcggtgt cactgctttt tctggtggtt
    72661 ttgtccctgc gtttgaagaa ttgacttccg gacaaggatt agctggatta tacgaaacct
    72721 tgcaaaaccc ggcaatgatt gcaatggtcg gaccaactcc agcttcgaat gctgctgagt
    72781 atactttagg agcgatgtat gcaaatgaaa tgttgctttt ttgcagtttg tttgctgctg
    72841 tgatctctgt tttacacgtg gtgagccaaa cgagaaaaaa agaagaactt ggtctgattg
    72901 aacttattcg ttcctttcga gtcggacgtc aagcaaactc tttagctgtc atcgctgaaa
    72961 gtattctgat caatttgctt ttagcaatat cgatcagttg tacgatgatt tttttccaag
    73021 cggattcgat cactgcagaa ggtgcttggc tttttgggct atctgtaggt agtatgggga
    73081 ttttggggag tgccgttgca ctgtttgctg cacaactgat gccaagttca gccagttcga
    73141 atggtctgtc tttagcgttt ttaggtggac tttaccttct acgagcaata acagatgtag
    73201 ctgattcctc cctttcgatg tggaaacctt taggctggtc ttatttgacc tatccattta
    73261 caactaacaa ctgaattcct ttgctttatt tgtttttgtt cagtctaatc ttgataatca
    73321 gttcatttgt tttagaggaa catcgagatt tgaacaccgg ctatatcccc gaacgtgaag
    73381 gaaaaccgta tgctaaacgt tctcttttat ccttgcgtgg tttattattc tatctaaata
    73441 aagggatgac aatcagttgg cttatcactt ttggtttgat gggggcggca tacggctcta
    73501 tctacgggaa tatgcaacag tttttagaaa gtaacgactt aatgaaacag atgtttatcc
    73561 aatctggcgt ctcaattgaa aaaaacttta caagcacgat catggtcgtc ttgatttgtc
    73621 tagctgctat tttaccaatt gctagcatca atcgtttatt tacagaagaa acccacggac
    73681 gtatgaatca gctgttcgca acaaaaattt ctaggaacca actttattgg accaatatgt
    73741 tactagctat tctatttgct atattggcaa tcctgattag ttctggtgca ctagggcttt
    73801 ctgctttggc ttcgatgaag gatgcgaaag acatggatct attgatgttt cttaaagccg
    73861 ggttcaattt cctgcctgcc gttttagtgt ttatcggaat tagtggcttt ctattcggat
    73921 ggcttcctcg aatcggtaaa cttgcttata tctacttagc ttactccttt gcactcaatt
    73981 actttggcgg aattttggat ttaccagaat ggttttccaa aacagctgtc cctagctggg
    74041 ttccacggat gccaaacgat tcttttgatc tttccgtgtt ccttacgtta tcatgtatca
    74101 gccttgcact gttcgtcctt ggttctattg gttataggcg aagagactta ctagaaggat
    74161 aataaaaaag catgtacaat ttcatgctag cctccaaatc tgggtcaaaa atccaacatt
    74221 tggaggtcat ttttttattt acaagtcttc taactgctct ttatcaatga tccagacttc
    74281 tcgattcttt ctttttacat aaccattctc ctctaataat ttaaattttc tagataaagt
    74341 ttcgggggtc gtaccgataa atgcagcaag ttctttcatt ttcattggca ggaaaaagtg
    74401 atccttccct gtcgctttag ctaagtcaag cagatagaca gcgactcgtt cttctacttt
    74461 ttccatcatc aaaaaagttg tctgctgctg tacttttgtt aatttctcaa cgttgattgc
    74521 taaaagtttc agactaaggg cgggataatg cagtaagagt tccgagaaat ctttttgtct
    74581 caatatgcaa atttccgttt tttctagtgc ttcgccaaat aaagactcat ttggaacgcc
    74641 tagcaactgg ctctctccct catatccgcc cggctctatt actcgtaaaa gctgttcctt
    74701 tccgctagca gataattgat acactttcat actgccggag gccacgatca ctaattgagg
    74761 ttctccattc ggcgcaagaa tctgttctcc tttttccaca tagtgatgtt tgacaagtgc
    74821 gtgtactttt tcttgatctt gctgtactaa gtgattgaac aaaggaacaa atgaaacaca
    74881 taaatgatca tgactcataa tctctcctcc ttctaaacag ctatttctaa taaagaaaca
    74941 taacttccct tattccaact gataattttc ttttatcata gcataaacgt caagcgaacc
    75001 agataccagc aaatagagtg gatttctttt tgtcccttgc tttattttcc ttcttttgag
    75061 accttaagta attgattcct atcaattttt taaatattta ctttttgaat aatcaaaaac
    75121 aaaccttcac tcgcaagccg agagtaaggt ttgtttttcg cattgcttat ttcgtgttgt
    75181 ctattgaaat ttttccgcac ctagcttttg atatagtggt gctagttctt gtaaggtttc
    75241 agcggttatt tgctcaacgt gttcgatttc ttgcggatag aagaatagtt tacccatttg
    75301 aaaggttcgg tctctcatcg cttttttcaa ctcttcttgt ttctttgtat cggacaaata
    75361 gtcagacaaa gctaagtggt cgtcaagttc atgttctggt tgcggccata tgtaataatc
    75421 cttgatatcg actgttttgc tccactcttt cagtatcgca agcaactggt tatacgctgc
    75481 aatgctacca tatcgttctt cgcttttata atgttggtac atcaaataga tttggtattg
    75541 tttttgattc aacaaaactg ctagacaggc attctcgtct tgacgagatt cgcttcggta
    75601 cgcgctccaa aaatgactgc gtaaattcca gccattcgtc cagctttcga ttttaggttt
    75661 gctcatgccg tacatatcag gcaaaagtgc agctgtctgg agctgaatct ctttccattt
    75721 ttgccagtgt tctttatatt ctttcttgat ttgttcaatc ttatctgttg gatggctctc
    75781 tttgagctgt ttgaatgcgt agattggtcg atcaaacaac tgataatctg attttccaag
    75841 ttccattgtt tcgcctcttt ccgttatctt ttgtattcta cctgaatttt gcgatatgga
    75901 caaatagagg ttgtgaaaaa gctgttctgc atcaagtcat aagaacagcc aaacaaaaaa
    75961 ctgcgatgag catcaccgca gttttattga acttaaggtt gattgatcac atctaaaagc
    76021 gttgaaaata aaatggcact agaagcagct cctgggtcga tatgtccgat tgcacgctct
    76081 cctagatagg aagctcgtcc ttttttagct accaagactt tggtgtgttc ttttgcctgg
    76141 tcaatgattt cttttgttaa attccctgcc ttcaatgcct ctgtgactgg cagccaaaca
    76201 tctaacatcg ttttatcgtc agcttctgcc tttcctcggc tttgaatacc cgctgttccc
    76261 tgctcgatca cttcgcctaa tttttcttga gaatcaatag aatcgacctc ttttgtcgct
    76321 ttcatcatgt tcatgaatgc agatccgtat aacggaccag aagccccacc gactttcgat
    76381 atcatcgcca tagagaatac tttgaatgta tcgctgattg tttcaggatg atcggcttga
    76441 aaagcttctt cataagctga aacccctctt cccatgttat ttccatggtc tccatcgcca
    76501 atcggggtat ctagatcact gagatattgt ttgttctgat tgattgcctc tgcaaattgc
    76561 gtgagccatt cttggatttc ttgaacggtt agtttcatat tatcaccctt ttatcttgag
    76621 attttttacc atgaaatcgt ctgtacattg ctttctaaag aatctttcca ttgtgtctcc
    76681 gtcaagtcga tcagtgttag agaaagacct tgcatatcta gtgaggtcat aaaattaccg
    76741 acttttcgga aaactacatt gacgcctctt tcttccaaca gagctaaaac atcattcatg
    76801 aacacgaact gttccatcaa aggcgtcccg cccattccgt tgactagtac acctgcttct
    76861 tttggtaaat tcgagtaatc atctaaaatc ttagtaacaa gttcctttgc cagtacttta
    76921 gaaggctgca tcttttctcg acgatagcct ggctcaccgt ggataccaac accatactcg
    76981 atctcatctt ctcctagttc gaatcctggt ttccctactt ctggtacagt tgctgctcga
    77041 agcgctagac cgatcgtctt agttgcctgt acgacctttt ctcccagttc tttcaactca
    77101 gctagggaag caccgtttct tgctgcatcg cccacgattt tgtgtacaag gacagtacct
    77161 gcaactccgc gttttccagc agtatacgta ctattttcaa cggcgatatc atcatctacg
    77221 atcacacttt ctacttcaat atcgtccata gcagctaatt cctttgccat atcgaagttc
    77281 aatgcatccc ctgtataatt cttaacgatc agcaaaactc cttttccact atcggcttcc
    77341 ttaatagccg tctgaatctg atctggagtt ggagaagtaa acacatcacc aagcaccgct
    77401 gcacttaaca tcccttcccc tacaaacccg gcatgggatg gttcatgtcc gcttcctcca
    77461 ccagaaacca aaccaacttg ttccgctttc tgattcttgg ctacaacacg ggaagattct
    77521 acacgatgaa caagttctgg atagcttttt actaatcctt ccagcatttc ttcgacaata
    77581 ttgcttggat cattcatgat ttttttcaat ttattcccat cctttctttc cggcattttg
    77641 tgcttctgtt aagatttgct gagtatcatc tgttataccg gcagtgatgg ctgctgcaaa
    77701 agctccttcc actaaaggcg catccaccag atgataacgt tgctgctgct cttcttccaa
    77761 catgtctttt gccagttccg agttcaatac cgcgctaccg atatccgcaa aaacaaaata
    77821 actagctgca tcgcttgatt cattgactgc ttctacgatt ttcatcgggt ctgatccgat
    77881 ctcgccattt attccgccga gagaaaaaat ttgcacctct tcggattttt gcatctgctc
    77941 aatcatttct ttaataccat ctgttacttt ttgactatga gaaataagca aaatactttt
    78001 tttcacaaga atcttccttt cttttacata gttgctaacg attggtttcg ctttcattaa
    78061 tgaacaattc aagtatataa taaatttcta attttgggaa aatatgtgta gtgcttcttg
    78121 aagtttgtta tactttaatt aaaagagagg agtgatcgaa tgattacaac aacaacgaac
    78181 agtgtagagg gaaaagccgt agtagcttat aaagggatcg tttttggtga ggtcattact
    78241 gggatccatg catttaaaga cttggaggcg gggttgcgaa atatctttgg cggccgctca
    78301 aaaagctatg aaaacgaact aatgggtgca agagaagaag cacttgccga aatggccgaa
    78361 cgtgcaaaag cattaggagc tgatgcgatc attggcgtaa aaatggacta tgaagtatta
    78421 ggcagcgaca atggcatgct gatggtcaca tgtagtggaa cagctgtctc cacgagataa
    78481 gtaggggaca tctgttttaa taactaaaga gagatgggac atgtagtggc actcaagaat
    78541 tagatttttc agtctaactt cgaggtactc taccttccca tcctctcttt tttatttctt
    78601 tttgcggatg attccgtaag ctgctgcgac taacacaccc gcccctgcta aggaataact
    78661 gacagaagct gttttttcat ttgtttttgg caaagtctga cttgttgatt tggatggctc
    78721 tgtcgtttgc tgaactgttt ctgtcgtgct tccagaggtc actgattttt ctcctgccga
    78781 atgcaaaata tcttttattc gttggaaact agctgctaag tcagcttttt ttgcttcaaa
    78841 atcattagca actgtttgtg cttcgctgat ctttccgtgg attgcttgac tggccgctgt
    78901 gatatccgct tgagtttgac ttagctttcc agaagctgtt tgagcaacct gatttaattg
    78961 ctgattgaat tcttttgctt gttggatctt cccgtcaaga tctgtttgga cagcttccgc
    79021 ttttgctttc gcttcggcaa gctttttctc aagttgtgca cggtcatttc ccactttatt
    79081 tttaagatca gctaaatctt gatcgattgc tgcttggtct tctttcaatt tgttttttgc
    79141 agcttctaat gcttcttcga gttcttttct tttattttcc aagtcttgca aatgatcgat
    79201 ttggtcagaa gaggtagaac tatttattga tgtatcttct gaagatgaaa tcgtttctcc
    79261 tgaattatct gtcggtacat tggaagtcat ttctgtttcc gaggcaccgt tttcagcacc
    79321 agaagagctt atggttgttg aaggttctgt ttgagcaggt tcagaagttt ccgcaaaagc
    79381 tgtctgtccc aataagaaaa tactggaaac agcaattggc cagattaacg gcttcattct
    79441 ttttttcatc acttttccca tccttactac tttttaacta atttcttatt ataagcaaaa
    79501 atgtttggaa gaaccataaa cacaacaggc tgtttgtcta aaaaacggtg catcttttat
    79561 ttttttgtac gttccgggct tttccatgtc ccgcttccat aacctaaaat cgttttgact
    79621 gctggaatga aagtcgtcac gatcgttatc gcgttcacta tccagaacaa aagcatatat
    79681 agtggtgcga aaagaaggta tttcaatttt cttcgtcgat catctacaat cagcgaagct
    79741 agcaactgga aaaatcctgc gaccatttca aagcagatga acacaaaact gaatgtaaaa
    79801 gtaatataaa tctgttcgta gtttccttga tatacataat gcccgattaa tccagcaaaa
    79861 agaactacgg aaatacaaaa gaatattgac cagacaatac tcaatgtttg atcaatgaag
    79921 atgatcgttc ttccaatatg tttaaaagga tggagcatca cttttttgaa attcgtcagc
    79981 cagacttctg ttccaccttt tgcccaacgt tttctgtgtc gatatagcat cttcaacgtc
    80041 acaggaactt ccatgaaaaa gataatctca ggagcaaaaa cagataacca gtcatttaac
    80101 tgatgatccc aagcaatact gatttcttct gtcgctcgat cttgtctgaa tccgcccaca
    80161 tcgatcaagg cttcttttcg atacatcgta tttgctccac tgtacgcata aagaccaccg
    80221 aaaacagctg actgtgttcg tttaatgatg cccacgatac ttgagaattc aaccgtttga
    80281 gattttgcaa tcaatttggt acgattttgg acatccatat ttgcagtgac cgcagcaata
    80341 tgtcttgcac cagggcgaat gaaataattc acatacctaa tcagtgcatc tggctccggt
    80401 accgtgtctg catcattgct taaaatcaat ttcccttttg caaaggcaag tccgatatta
    80461 aacgcgtggg cttttccttt atttttttcg atccgaacca cacgaagatt agcgtatttt
    80521 ttcatcagtc tagctaatat ttctggtgtt tggtccgttg aaccatcgtc tgtcactaat
    80581 acctcatagt tgtgataatt caatttcgtc atcaaatact cgattgtata ttcgatcaca
    80641 atttcttcat tatgggctgg caccatgatc gtgatgaacg gttcgacact caagggaaca
    80701 tctacccatt ctttttgctt atgtttgtat ataaacacat aacaccagac accaataaac
    80761 catacaaatc cgcctaaaat cggataagaa acgagaaaaa aatacataat agacaaacaa
    80821 gtatctaaga ataaattacc tgtatgggtg attgagatca tttcagttcc accccatttt
    80881 ctttatacaa atctgcaatg aatgtcgtat ctagattttt ctcttcggag acagaataaa
    80941 aacggaccgt ttctctctct tcttttgttc caaaacgttc cgtatacact tcttcaagta
    81001 attcttgtct ttttttcaat ttttcatctt catattcaaa agatttattt acttttttgt
    81061 atagatttcg attgttccac ctagtcaata caaatgaggt aatcaggaaa ctgaaaaaaa
    81121 tcaatacaaa aaagccgatt aagaaataca gcattttctt ttctgataaa aaagcgtaca
    81181 aggaaaaatc cttggctctt tctggtctcg ttaatgggaa aaagacccat ataaaaggaa
    81241 gaacaacgat catccatcct aaaagagcca ctaacgtttg ccaaatcttt aaccagtaat
    81301 taccttttga aaaatactta tctttcacca aaattgatgc aacttcttct cttgttactt
    81361 ttttcatatc ctcacctcac gtttttttgt tacgacgtag gagtttacca tacatttcat
    81421 ttcttttcac ataaagtgtt cctaaatttt tcctaagaaa aagaacaaaa catttctcat
    81481 cagcaaatga aaaatatttt gttcttttta tcagttgttc tttttatttt ttatctctta
    81541 attatgttgc tctttccaat acgacaatgc ttttcattcc tctagatatc gccgtgtaca
    81601 tttgtctgcg tcttctttgc tcatctccat cgttttcaaa tggatataag attacatgat
    81661 caaattccag tccttttgct agactcaaag gaatgatttg gcagttttct aatccatatg
    81721 aatttttttt aatcgattct tgtagcgttt gggtttcctc ttctgtttgg gtgataactg
    81781 ccgctttttc tccttttaga gaaaccaaaa tctcagctag tattttcaac aattcttcag
    81841 aggatcgaaa agagcggtat tcgggttctt ttccttgcgg tcggatcgat acgatgtcga
    81901 tcttctcttg atccacagca taggttttga aaagctctgt gattgctccg ctagaacggt
    81961 aactatttct aagatcatac cgatggatcg gcagatggta gtcgtcgaaa caacgcataa
    82021 tatcagcaaa agtagtggaa gaattaaaga ttgcctgatt ctcgtcacct actaaagtaa
    82081 agtgagtttt tggaaataat tcgatcaata gacctaattg tgccagggta taatcttgta
    82141 cttcatcaat caatagatag cgaaatgctg gtacatttac cttttcaaca agtaaatgac
    82201 gaatagctaa tataatgact gcttcatcaa cagtacgtgt gggttgccaa gcataagctg
    82261 agccatatcg tttttcataa attctttcaa acaactgatg ctcatccacc catgccattt
    82321 cttcgatttg tctggttatt tttcgatatt ttttttgtaa aagtttcctt gcataagcta
    82381 caatactttg ttccgaatca tcagaaatca atttaccgaa taattccaac tgcctatctt
    82441 ctgtcaacga gagtacctga ttctgcacag caggactttt tgcttgtttc aaaagatgat
    82501 tcttccattc gctcatcagt gcctttttcg ttgcttgaag cctatcgatc aatttaggat
    82561 gagcaggaac tttctcatat attttttcga tcgttttttt agaaaggact gtttttccat
    82621 ttttagtaag tccacggata aaattcggat gatctaaaaa caaaggatct gattgtttta
    82681 gattatcgat aaactttttg ctacgcaaac gttctgtctg ctccgataca ttttctcccg
    82741 tgatacgttt gaaataagcc tcttcacctt ctatttcctc tgctgaaagc tgtgaaacca
    82801 gctgtatgat ggttagattc agtggatttc tttcacctaa tgatggtagg acattcgaaa
    82861 tatagtcgat aaaccgatga ttaggtgata gaatcagcaa gtcatcggat gtcatcgtat
    82921 ctcgatataa atagagcaga tacgcaatac gctgcatgat agcagaggtt ttaccacttc
    82981 ctgctacgcc atttaccaag atattttgac ttttcacatc ccgaatgatt gtgttttgtt
    83041 ccgattggat cgtagcagtg atagccttca tttcattggt ctcatcttgg cctaagactt
    83101 ctaataatac gtcatcttga atagctacag acgtatcaaa aaacttgacc aaacgatctc
    83161 tttccaaaag aaattgtcgt ctttctttaa taaccgtatc gattttattt tgacgaaccg
    83221 tataagaaga tggaccaatc atgttattgt aaaaaagttc ggcaattggt gaacgccaat
    83281 cataaacaag attttcatct gcatcatctg tgaaatttgc tgtaccgata taaaaggatt
    83341 cttttggctc attatccaga aagtctacaa cgacttttcc aaagtaggga cttttcaata
    83401 aacgttcgat ctttgctagc tgattttctg cgtttttctg tttgatattc atctgatcga
    83461 tttctctatt tttcatttca atcatcgaaa aagtatccag gttatctaag taactgtcaa
    83521 aattcaatcg aagctcgcca gccatttctt tcaacgactg gtttccttct atttttgccg
    83581 tatccatcga ttcccgcagt tcgttttttt tatcaattag cttttgatac acgttcttca
    83641 gatatgcttc ttctaactgt ttttgtgtgt tgaccataca cattctcctc tttttttaaa
    83701 gtggtggaac cgattagttt acctctttta agagtcgaat gcaagctttt taacgagtat
    83761 catttgttag gatatatcag ttcttcaggt cggacagttt gatataaaga aaatgcacca
    83821 tcaaggtttt ttacgtggta gccagactgc ttcaagattc tttccgctag atagctacgt
    83881 aatccgctat gacagctgac gatatattct tgttggctgt ctaattcatt taatcggtcc
    83941 cgaagttcat tcaaagggat atgtttggct tccttgaatt tgcccttttc caattcttct
    84001 gctgttcgca catctaataa aatttttcct tttgctagtt ctgtcggcaa ttcatgccat
    84061 tgtacggttt cactaagccc ttctgcaatg ttcattgctg catatcctag catgtttact
    84121 ggatctttgg ctgagccaaa tggcggtgca tacgtcaatt ctaattccgg taaatcgaag
    84181 atcgtcaaat gccctttgat tgctgttgct aaaatatcga tccgtttatc cacacctttt
    84241 gccccaacac cttgtgcacc ataaatctcg ccagtctctg gatggaagat caacttcagt
    84301 aaaatatctg ttgcttctgg ataatagctt gcatgatctt ttcctgatac atgaatgact
    84361 tcaaaagaca gttcgttttc tttggctgtt ctttcactta aaccggtgga tgcggcagtc
    84421 attccaaacg ttcgtataat tgctgttcca ataccgcctt gatttcttcg agaaattccg
    84481 gaaatcgtgt ccgcaacttg tctaccttga cgattggctg gtgaagcaag agaaataagt
    84541 gcatcttttc cagtgatttg ctgttttaca acgatagcat cccccaccgc ataaatatca
    84601 ggaatattcg tttgataacg ttcgtctact aggatacccc cgcgtatgcc taattcgatt
    84661 ccagcggtca ctgctagctg gttatcagga cgaacaccaa tggataaaat aactaaatca
    84721 gcagggatgg tacgcccatc atccagtcgc aatcgtttcc cgtggtcttc aaatccagcg
    84781 acagcatgag aagtaatcac ttctactcct tgatgagaca attctttttc tataaaagct
    84841 gccatttctt catccagtgt tggaagaata tgtggtgctt gttcgatcac catgacttct
    84901 aaacctcttc tttttaggtt ttctgccatt tccaatccga tgaatcctgc accaacgatc
    84961 acggcacgct ttggctgttt ttctaatgca tgcatcactt tatcgatatc tggtacattt
    85021 ctaatagaaa aaacattgtc ggcttctgtt atccctggaa tcgatggaac aactggtttt
    85081 gcaccaggag ataaaaccaa tgcatcgtac tgttcaatgt gtttttgacc ctcatgtacg
    85141 acttctaccg ttttgttttc gggaaagatg gctgtcactt catgatttgg acgcacatct
    85201 aaacaaaaac gagcggataa tgattctggc gtttggacaa gaagattttc ccgttcactg
    85261 atttctcccg aaagataata aggcaagccg caatttgcaa aagaaacata cggtcctttt
    85321 tcaaaaacaa cgatttctgc atcttccatt aatcggcgaa gccgtgtagc agcagacatt
    85381 ccacctgcta cacctccgac aataacgatt ttcatcttat ttccctccta ctgttcgatc
    85441 aaaccattga ttcatgccac cacgtacatt cactacatca taaccagatt tctttaattc
    85501 tttagctgct tgtttactcc tcatccctga ttgacaaatc acatagacgg tcttttcttt
    85561 gtctccatca tatctgttta tactttgtaa tggaacattt ttagctcctt ttatatgccc
    85621 gccccggtat tctgcaggtg ttctaacatc aagtaacgct ggattttcca aaagcttttc
    85681 ctttaattct gggactgtta tcgattttat tgattgaaat agcaaattca ttttctgcac
    85741 ctcatttaca tttttataaa tacctcatgg ggtatattaa acaggagctt tattaatatg
    85801 tcaaccagaa tgactcttca tttttttcac tactttttat atttgcataa aataccgata
    85861 aggggtatta tctaattata aaaatagagg agaatgtgtt atggagattg ataataaaaa
    85921 tatcctgaac cgtttgaaac gtacagaggg acaaattcgt ggtatccaaa aaatgatcga
    85981 agacgaaaaa gaatgtatgg atgtcatcac tcaattaagc gctgttcgtt ctagtattga
    86041 tcgcgtaatg ggcatgatcg ttgcagataa tctgaaaaat tgctttgaga aacctgatag
    86101 taatccagaa gaacaagcgg ctaaactaga acaagcgatc aagatgatta ttaaaaagta
    86161 aaggaatgat ttcaatgaaa gtcgttttcc atattgatga attagaaaaa tggtcagaaa
    86221 cagggaaaaa cgtaaaaaac ctgataaaag cttctccaga aacgacgatt gtcgtatctg
    86281 taaatggcat cgcgattacc ggttatctag actctgccaa tgcagaattc ttagatttgc
    86341 aaggagtcgt ttttcacgct tgcgccaatg ctatgcgtgc taatcatatt tcagaaagtt
    86401 ctttacctga acaagtaatc gttgtaccag caggtgtcct tgatttagtt gaactgcaat
    86461 cccaaggtta tgcttatatc aagccttagt cagtagaggt tgcgacagaa gtgatcagct
    86521 tcaagaaatt tcggcttatt tttcgaagaa gctacttctg gaaaaccgtg aatcaaaggt
    86581 tgtgaaaacg ctgtcctgca tcaagtaata agaacagcca aacaaaaata gcttctcata
    86641 tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg tttaaggaag
    86701 aggttgtgaa atcagcggtt tgacctaagt attagagaaa aaaacgggaa aatagctcct
    86761 catattttgc cgttttttga gctaataccg aaggtcagct gattgaacac cgtttaccaa
    86821 gaggttgtga gcctaccatt taactccgaa tcacaagaaa taatttcaaa aacaacttct
    86881 cctgtttttg gtttggttgg cttattatcg agaagtagct actgggagtc tatttatcaa
    86941 aaaaaggaat gcgacatagg tcacacccct ttttt
//
LOCUS       DACKAC010000004        85311 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.004, whole genome shotgun sequence.
ACCESSION   DACKAC010000004 DACKAC010000000
VERSION     DACKAC010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 85311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 85311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..85311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.004"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(93..521)
                     /locus_tag="I0B46_000272"
     CDS             complement(93..521)
                     /locus_tag="I0B46_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2082603.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(549..1187)
                     /locus_tag="I0B46_000273"
     CDS             complement(549..1187)
                     /locus_tag="I0B46_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAR2082604.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(1191..3227)
                     /locus_tag="I0B46_000274"
     CDS             complement(1191..3227)
                     /locus_tag="I0B46_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAR2082605.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERVISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            3353..4018
                     /locus_tag="I0B46_000275"
     CDS             3353..4018
                     /locus_tag="I0B46_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAR2082606.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDF
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            complement(5953..9102)
                     /locus_tag="I0B46_000276"
     CDS             complement(5953..9102)
                     /locus_tag="I0B46_000276"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HAR2082607.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(9115..10314)
                     /locus_tag="I0B46_000277"
     CDS             complement(9115..10314)
                     /locus_tag="I0B46_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAR2082608.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLIDLVVRLNKSTNSSRLPKVEF
                     EDIVAGEGRLNKDVSQKFDNRKGIEFLPNDILYGKLRPYLKNWLKATFTGVAIGDFWV
                     FRAKKSDSDFIYSLIQADRYQKAANDTSGTKMPRSDWKKVSDTVFYIPNDLKEQQKIG
                     IFFKKLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWEERKLGE
                     LGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIERKKWKPI
                     KDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFETINLPR
                     YSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLLKETKKS
                     FLQKMFV"
     gene            complement(10304..11899)
                     /locus_tag="I0B46_000278"
     CDS             complement(10304..11899)
                     /locus_tag="I0B46_000278"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAR2082609.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEMWLQGALEVFKHEQ"
     gene            complement(12483..13190)
                     /locus_tag="I0B46_000279"
     CDS             complement(12483..13190)
                     /locus_tag="I0B46_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2082610.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENG"
     gene            complement(13255..14091)
                     /locus_tag="I0B46_000280"
     CDS             complement(13255..14091)
                     /locus_tag="I0B46_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAR2082611.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(14109..15518)
                     /locus_tag="I0B46_000281"
     CDS             complement(14109..15518)
                     /locus_tag="I0B46_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAR2082612.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(15532..17355)
                     /locus_tag="I0B46_000282"
     CDS             complement(15532..17355)
                     /locus_tag="I0B46_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAR2082613.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(18198..18524)
                     /locus_tag="I0B46_000283"
     CDS             complement(18198..18524)
                     /locus_tag="I0B46_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2082614.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(18570..18932)
                     /locus_tag="I0B46_000284"
     CDS             complement(18570..18932)
                     /locus_tag="I0B46_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAR2082615.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(18969..19184)
                     /locus_tag="I0B46_000285"
     CDS             complement(18969..19184)
                     /locus_tag="I0B46_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAR2082616.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            19311..19646
                     /locus_tag="I0B46_000286"
     CDS             19311..19646
                     /locus_tag="I0B46_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAR2082617.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(19722..21719)
                     /locus_tag="I0B46_000287"
     CDS             complement(19722..21719)
                     /locus_tag="I0B46_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2082618.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(21817..22092)
                     /locus_tag="I0B46_000288"
     CDS             complement(21817..22092)
                     /locus_tag="I0B46_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAR2082619.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            22324..23058
                     /locus_tag="I0B46_000289"
     CDS             22324..23058
                     /locus_tag="I0B46_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAR2082620.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            23097..24239
                     /locus_tag="I0B46_000290"
     CDS             23097..24239
                     /locus_tag="I0B46_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAR2082621.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(24334..25725)
                     /gene="sufB"
                     /locus_tag="I0B46_000291"
     CDS             complement(24334..25725)
                     /gene="sufB"
                     /locus_tag="I0B46_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAR2082622.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(25730..26200)
                     /locus_tag="I0B46_000292"
     CDS             complement(25730..26200)
                     /locus_tag="I0B46_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAR2082623.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(26187..27422)
                     /locus_tag="I0B46_000293"
     CDS             complement(26187..27422)
                     /locus_tag="I0B46_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAR2082624.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(27419..28705)
                     /gene="sufD"
                     /locus_tag="I0B46_000294"
     CDS             complement(27419..28705)
                     /gene="sufD"
                     /locus_tag="I0B46_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAR2082625.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(28718..29491)
                     /gene="sufC"
                     /locus_tag="I0B46_000295"
     CDS             complement(28718..29491)
                     /gene="sufC"
                     /locus_tag="I0B46_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAR2082626.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(29688..30536)
                     /locus_tag="I0B46_000296"
     CDS             complement(29688..30536)
                     /locus_tag="I0B46_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAR2082627.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(30560..31246)
                     /locus_tag="I0B46_000297"
     CDS             complement(30560..31246)
                     /locus_tag="I0B46_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAR2082628.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(31239..32273)
                     /locus_tag="I0B46_000298"
     CDS             complement(31239..32273)
                     /locus_tag="I0B46_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAR2082629.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            complement(32668..33006)
                     /locus_tag="I0B46_000299"
     CDS             complement(32668..33006)
                     /locus_tag="I0B46_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAR2082630.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(33008..33364)
                     /locus_tag="I0B46_000300"
     CDS             complement(33008..33364)
                     /locus_tag="I0B46_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAR2082631.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(33370..34557)
                     /locus_tag="I0B46_000301"
     CDS             complement(33370..34557)
                     /locus_tag="I0B46_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAR2082632.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPVRS
                     SRR"
     gene            complement(34748..35593)
                     /locus_tag="I0B46_000302"
     CDS             complement(34748..35593)
                     /locus_tag="I0B46_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAR2082633.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            35827..36792
                     /locus_tag="I0B46_000303"
     CDS             35827..36792
                     /locus_tag="I0B46_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAR2082634.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            37169..38212
                     /locus_tag="I0B46_000304"
     CDS             37169..38212
                     /locus_tag="I0B46_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAR2082635.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(38274..38738)
                     /locus_tag="I0B46_000305"
     CDS             complement(38274..38738)
                     /locus_tag="I0B46_000305"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAR2082636.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            38984..39931
                     /locus_tag="I0B46_000306"
     CDS             38984..39931
                     /locus_tag="I0B46_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAR2082637.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(40142..42214)
                     /locus_tag="I0B46_000307"
     CDS             complement(40142..42214)
                     /locus_tag="I0B46_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAR2082638.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(42147..42524)
                     /locus_tag="I0B46_000308"
     CDS             complement(42147..42524)
                     /locus_tag="I0B46_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAR2082639.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(42653..43213)
                     /locus_tag="I0B46_000309"
     CDS             complement(42653..43213)
                     /locus_tag="I0B46_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAR2082640.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            43363..44655
                     /locus_tag="I0B46_000310"
     CDS             43363..44655
                     /locus_tag="I0B46_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAR2082641.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(44715..45833)
                     /locus_tag="I0B46_000311"
     CDS             complement(44715..45833)
                     /locus_tag="I0B46_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAR2082642.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(45847..47268)
                     /gene="arcD"
                     /locus_tag="I0B46_000312"
     CDS             complement(45847..47268)
                     /gene="arcD"
                     /locus_tag="I0B46_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAR2082643.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(47514..48635)
                     /gene="mnmA"
                     /locus_tag="I0B46_000313"
     CDS             complement(47514..48635)
                     /gene="mnmA"
                     /locus_tag="I0B46_000313"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAR2082644.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNDKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            49039..49638
                     /locus_tag="I0B46_000314"
     CDS             49039..49638
                     /locus_tag="I0B46_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAR2082645.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(49776..51062)
                     /gene="efmA"
                     /locus_tag="I0B46_000315"
     CDS             complement(49776..51062)
                     /gene="efmA"
                     /locus_tag="I0B46_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAR2082646.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(51333..51677)
                     /locus_tag="I0B46_000316"
     CDS             complement(51333..51677)
                     /locus_tag="I0B46_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAR2082647.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(51814..52950)
                     /locus_tag="I0B46_000317"
     CDS             complement(51814..52950)
                     /locus_tag="I0B46_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAR2082648.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(53139..54113)
                     /locus_tag="I0B46_000318"
     CDS             complement(53139..54113)
                     /locus_tag="I0B46_000318"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAR2082649.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(54193..55068)
                     /locus_tag="I0B46_000319"
     CDS             complement(54193..55068)
                     /locus_tag="I0B46_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAR2082650.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSEKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            55198..57105
                     /locus_tag="I0B46_000320"
     CDS             55198..57105
                     /locus_tag="I0B46_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAR2082651.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(57646..60924)
                     /locus_tag="I0B46_000321"
     CDS             complement(57646..60924)
                     /locus_tag="I0B46_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAR2082652.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(61195..61740)
                     /locus_tag="I0B46_000322"
     CDS             complement(61195..61740)
                     /locus_tag="I0B46_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAR2082653.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(61752..62444)
                     /locus_tag="I0B46_000323"
     CDS             complement(61752..62444)
                     /locus_tag="I0B46_000323"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAR2082654.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(62463..62747)
                     /locus_tag="I0B46_000324"
     CDS             complement(62463..62747)
                     /locus_tag="I0B46_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2082655.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(62760..63323)
                     /locus_tag="I0B46_000325"
     CDS             complement(62760..63323)
                     /locus_tag="I0B46_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAR2082656.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            63551..64213
                     /locus_tag="I0B46_000326"
     CDS             63551..64213
                     /locus_tag="I0B46_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAR2082657.1"
                     /translation="MIKRNWPWLLTLVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            64337..65533
                     /locus_tag="I0B46_000327"
     CDS             64337..65533
                     /locus_tag="I0B46_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAR2082658.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIAFTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(65634..67010)
                     /locus_tag="I0B46_000328"
     CDS             complement(65634..67010)
                     /locus_tag="I0B46_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAR2082659.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(67047..67913)
                     /locus_tag="I0B46_000329"
     CDS             complement(67047..67913)
                     /locus_tag="I0B46_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAR2082660.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(68002..69027)
                     /locus_tag="I0B46_000330"
     CDS             complement(68002..69027)
                     /locus_tag="I0B46_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAR2082661.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(69045..69719)
                     /locus_tag="I0B46_000331"
     CDS             complement(69045..69719)
                     /locus_tag="I0B46_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAR2082662.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(69903..70796)
                     /locus_tag="I0B46_000332"
     CDS             complement(69903..70796)
                     /locus_tag="I0B46_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAR2082663.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(70870..72567)
                     /locus_tag="I0B46_000333"
     CDS             complement(70870..72567)
                     /locus_tag="I0B46_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAR2082664.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(72560..73873)
                     /locus_tag="I0B46_000334"
     CDS             complement(72560..73873)
                     /locus_tag="I0B46_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAR2082665.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(73873..77391)
                     /locus_tag="I0B46_000335"
     CDS             complement(73873..77391)
                     /locus_tag="I0B46_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAR2082666.1"
                     /translation="MDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEK
                     KEISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMV
                     NFYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETL
                     YKDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDV
                     LFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGS
                     SNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAI
                     GRNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVS
                     PFGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYK
                     FSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSS
                     SIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFET
                     KMPILGDETGAISDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEY
                     TSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNI
                     NKLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDG
                     KYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRL
                     YDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTL
                     DGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQP
                     LHLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIID
                     GVLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPND
                     SSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTL
                     KVEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYIL
                     LAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(77684..78142)
                     /locus_tag="I0B46_000336"
     CDS             complement(77684..78142)
                     /locus_tag="I0B46_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAR2082667.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(78440..78958)
                     /locus_tag="I0B46_000337"
     CDS             complement(78440..78958)
                     /locus_tag="I0B46_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAR2082668.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(78960..79577)
                     /locus_tag="I0B46_000338"
     CDS             complement(78960..79577)
                     /locus_tag="I0B46_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAR2082669.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(79574..80428)
                     /locus_tag="I0B46_000339"
     CDS             complement(79574..80428)
                     /locus_tag="I0B46_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAR2082670.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIILLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(80425..81336)
                     /locus_tag="I0B46_000340"
     CDS             complement(80425..81336)
                     /locus_tag="I0B46_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAR2082671.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(81377..82711)
                     /locus_tag="I0B46_000341"
     CDS             complement(81377..82711)
                     /locus_tag="I0B46_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAR2082672.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            82919..83749
                     /locus_tag="I0B46_000342"
     CDS             82919..83749
                     /locus_tag="I0B46_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAR2082673.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(83993..84242)
                     /locus_tag="I0B46_000343"
                     /pseudo
     CDS             complement(83993..84242)
                     /locus_tag="I0B46_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(84433..84627)
                     /locus_tag="I0B46_000344"
     CDS             complement(84433..84627)
                     /locus_tag="I0B46_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2082674.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
BASE COUNT        24926 a        17907 c        14715 g        27763 t
ORIGIN      
        1 caactaaaaa tgaatatatt aaaactacca aaaataactt ttaacgctgt ttcattatat
       61 gttttttcta ttttatataa acttttggat acttaatacc aaccggttgg attgtaccaa
      121 catgagaatg tgtcagttct atttcctgtc gcacttgcat aggcccatac tccttttgtt
      181 gcatttgctg atcgaataga gtttccaact ttcgcttttg caccgtgacg attatagaca
      241 cgactatagt ggttagaatg acctgttttt ctcgtacaag ttgtatcact ataataaacc
      301 catcttccag agctatgatt aattgctgtt ctagaaattt cccggtctac tggtaaattt
      361 gacttaataa tattaccttt agcatcaaaa tgaatctctc ctggctttaa agatttgtaa
      421 tagatttcac cgtgaactga atttgcttcc acttcagatg acatagcgtt tcctccaaca
      481 aaacctatcc ctagtgcaca aataataact gcttttttca taaaacgaca tcctttcctt
      541 tttgattttt aaagcatgat cttcttgttt cctattttgg tcagatatgg atcatgcgtg
      601 actacgataa ctaacgtacc ttgggaagct tgttgcgata agatttcaac tactttatcc
      661 ttatttcttt tatccaatga cccagtcggt tcatcggcta aaatgatttt gctattcttc
      721 aataagactc tcgcacaggc gattctttgc ttctcaccac cagataatgt gtagataggc
      781 gtacttagct tcttgtttat atttactttt ttcaatgcct catactttgc tgttcgctta
      841 tctgaaacat ttaaattcga aaacttcaat ccaatatcta aattaaaatc gattgtttca
      901 tattcaatca acccaaaatc ttgaaaaaga aaagatatgg tatttctttt tatcaagcgc
      961 ttctctctcc cagttaattg tttcgtctct tgatcgaaca aatagaattg tcctgaactg
     1021 aactgatcat tcaatcctaa tatattcaat aacgtacttt tacctgctcc actttctcca
     1081 acaacagtca caagatcccc agcatccgca gtgaaacgat ccaaatgaag aatcgttttg
     1141 tttcgaatga tttttttcgc accttttatc actatcaacg gctccaattg ttaacctcca
     1201 ttcatcagca ataactgact tttcttttct gtagacaaaa taaaaacaac tgaaagaata
     1261 aaatcaatga agctaaagaa aaaaatatta aaaagatatt taggctctct ttctataaga
     1321 aaaaggaaga ttacacttgt cgttccccat ttgatgaggt tcatcatgaa gaaatcttta
     1381 tacgtttcaa agagcgaata accacttgaa cgaaagatag cgattttccg acaattgtag
     1441 acaaaatata tgcataacat gacatagctc aacaagaaga aagaaaagaa agtgagtaaa
     1501 aatatctgta tcaagtaatg aaatgatctg cttaacactt tgacagctat ctcttcagtc
     1561 tgagtatacg gaaatgataa ctgcaagtta tcacgtaact gactttcttc aatcagctca
     1621 aataaccttt ctttcgacaa agagcctatc ttgatcttta acggtggata gatttctccg
     1681 ctgagaatat ttctttccaa atacatactg tttttcaatg tcagtatttc aacaaccgga
     1741 taatgttcga tccaggggat agaaggaata aatgtataaa tgggttgatt gttttttata
     1801 aatagaaatt tcggttcttt ttcactgatt tcttgataat attctgttat ttgcgccagg
     1861 tcatttttta aatctgtatg tctatatcgt tcagggatca atataacagg gtgcgtttct
     1921 ttttcggtta tttggatcaa ccttccagag acatcaatca aaggaaattt cttaagataa
     1981 ttcagattta tttttatccc tctgccaaac tgttcatttt cttcattcaa gtaatctgat
     2041 acattgacta atatacttcc gtttttattc aaagtttcat ataattcctg atctgctgct
     2101 gatgcaaaac gattggaata aataaaatcc acgtggtttt tcccgatggt tactggataa
     2161 aatacatgat agtcctcacc agcgatttct tttggtggtt catgggaaac agctaactgt
     2221 gaagaagaaa aaacgatagt tgctaattcg acattggcaa cggtcaaaag aagagaaaac
     2281 aacaatccaa atgtaaaaag aatgtattgg atatagttga acgcttctct taacgaagcg
     2341 attttttcaa ctaataaaag agaaatcaaa gagataccat atattccgct gaacaagaaa
     2401 acgtacatct ggaacaaata aagaaattgc tccttataat acataaaaat aaaaccacca
     2461 atgattcctg caattatgaa tgtaataatg attttctttt tccccaataa acaccacaat
     2521 acttgatgta atgaaaagtc attattcaac aataaactga tttctttatt catcaataag
     2581 aaaaaggatg ataacaaaag gaataaaaga atcaatccaa tagttaaata cactcgataa
     2641 ttatatacgt ctgtatttga aataaccgaa tacgaatagc tattttcaaa gtcttcaaaa
     2701 atattagtgg cataatcagt gtgaaatgct tgattgaatc gatcagtgaa tagtgaaatg
     2761 acccgttcat aggcttcttt ttctctcgtc atcaagaaaa attcaccttc aaaatctttt
     2821 attttgatcg attcttcaat ggacgttacg cgaaaggagg tattagaata gggtaccttc
     2881 tcgtgagaag atttgacatc cccttgataa tacaatgtca tttcaagctt tggctgtatc
     2941 ttgaagttcc aagatgtatc tacttcattg gattcattta ctgttttttt catataagaa
     3001 agctcagttt ctgatgagac attttcaaat accatgatta gctgctcata ttcttcttgc
     3061 ccaataaaat gttgtggcaa tcgaatcgga gtataagtat ctgtttgatg gaaaaattca
     3121 tttgtccgat agagtacctg tttatttata ctaaccaagg caaaaccact catgataccg
     3181 gtcactatga ttgtgagtaa aactgtgaac ttttttttga ttatcaccac tctcccttac
     3241 ttaaatgatt ctttgataaa atcataccgt ttttcatcaa aaaaacgtat ctatcaagtg
     3301 tataggtcat gtagaataaa tctatatact gtagaatcga aaggagaaga agatgaacac
     3361 cggagaaaaa atacgaactt accgcagaaa ttgtaagctt acgcaaaaag aattaggcga
     3421 aaaaattggt gtttccgata aaacgatttc tagttgggaa aattctcgta caatgccaga
     3481 ctttgaaatg ctgtcacttt tacatcatac tttaggtctg ccgattgatt ttccttctat
     3541 tgaatcagct accgaatcaa tagaaccaac attgccagat gaacctcttg ttaacaagaa
     3601 atcttattca tcaaaacaac ccttctcact caaaaagaca ctatctttct ttctgctcgt
     3661 cttttgtttg gtctatcttt ttcttatcaa ttatcaatac aaaaatgaaa cgatcgatcg
     3721 attcaatcct tttttgaaac cacaaatcgg ctataccaca ctccctgtag gatacgtgga
     3781 caattccatc gaacgttggg tatgtaatga tatctttggc catggtacat ggctgtcttt
     3841 tcacgctgga gaggtagaaa aagagaaaaa ccatgctgta atcaaacaca aaggaaaaca
     3901 cgtcatgaac gttcgcctgc tcacttggga agccattccc tcagattacc aagaaatcct
     3961 cagccaagac tatgacgacg ccttaaaaag aagccctcag gagccagata ggcattaagt
     4021 aggagatgaa cgtagagata gaaaaaatca atgcacatga tcttatccaa aaaacagggg
     4081 tacaaattaa ttgaaaaagc cttgagatga aagttcctaa ataaaaaagt tcagaatgat
     4141 aggtatttct ggcttatagt ataatattta cgccacagcc tcccaatgat tctttttgga
     4201 atcataatca aggatggaca ttcacaaaaa tattttgctc attcgatccg aatagattaa
     4261 cgaccaatct agtaagtaat atagattggt acgtagtcaa gctgcacata atttagatca
     4321 attttagttc ttttttatat ttctatgatc tgctcattta cttttatatg atggcttaca
     4381 gtaacataaa taaatatgat aatcgctggt agtttatcca agagtactca ataaaataca
     4441 aaatggattg ttcaagcgcc tatcataatg ataaaatgaa acaaataaac gatacaagga
     4501 ggaagagact ttgagtaatg gcagaacaat tttttcattg taaaaattgt tcagttgtta
     4561 atctatggga tttcggtagc atgggcattt ttttatagag gaatcacacc aacctggccg
     4621 ttttatctct tgttagtggg aatattagta ggaaattttg cccctcttca ctatcgtacg
     4681 aacttttttc caactgattc tggccttttt cttaaaacaa agacgcgaaa attagaaaac
     4741 attattgaaa tgacaattac ctgtataatc attttcttag ttatattcct aaacgtcttc
     4801 taataagaat ctcttaaaat tagcttgtta cagttagtta cctagtttat ataacacagt
     4861 tatcttatct tccttatcaa catcttttgc caactctttg tcttaaattc gaaacatata
     4921 agcagagcta cgaacaccaa gactgtttcg gctctgtttt ttgcatcaaa ttcactctta
     4981 tatagctgat aatctcggct ctttaaaaga aattattttc gcttcatttt attataaaaa
     5041 ggctaggaac cttgatgtta tcaggttcct agcctttatt taagtattac cccacagacc
     5101 cttccatata gatttttaaa ttacacacta gtttaaacta aaacaaacaa ggaaaaactt
     5161 accatttaat caaaaaatta gctcatatga atatattaga atgtaaatta gactggtcaa
     5221 aatctggtca aaaaagagga cgaatacagg tagtcctctt ttttgttatt ttgatattct
     5281 aaatcatttt ttattggatt attcccttgt tcgatataga cccaaattga tctatcaata
     5341 attatcaaac tgagtcatat agagacaaaa atttcaaaaa tgacctgtta aatggtcatc
     5401 gaatatcatt taaaatcaat acgacggagt cagacacgga gtcaaaacaa gcccgctgag
     5461 agtaaaaaat atacagtccc ataggtaaag tgccacagga ctgtatattt ttttatttga
     5521 gattacctga aatgattcca tttaattttt atctcatttt ttcctactag ttagtatcaa
     5581 atactctcct attttctcaa taaactattt tgggttacat ggtatctgaa acagctaaaa
     5641 ccttgtcatt atagcaaaaa atcactttat ctccgtttca tttcagatac atttcactta
     5701 agcggattcc aaaaaaacaa aagaaaaagg aacccctagt tagcctatgc tttttattgg
     5761 ttcctctcaa aacacggtca tataacaacg gggtggggta aatgaatgaa aaaggtggag
     5821 gaaacattat tcaatagctg tcttaaatac ttcgttataa aatgtgaaca aataaaagac
     5881 atctatccct attttctaca cttccacttg actaccatct ttgaatcgta caattttctt
     5941 aactcttctc gtttacctca attcattctc aagtggaatc aactcatcat ctaacatttt
     6001 acgccatgct cgcttcattt cttgtgggta tttaaatggc ttcgcttctg gatgtttggc
     6061 tttatatcct tcgtaatcct tactgttgat aattgctttc atattaggaa ttggatcttc
     6121 acctggtaga tattgcagat tagaagcatg taattctttc tcagaaacaa accactcatt
     6181 ggaaaagaca tgaatcaaat catcactcgc ttgtgtaaaa tagcgacgtt taatgacaaa
     6241 gatatcttca ttactatcta aagtccccac atcaaatgca tttaaaattt gtcggtaaac
     6301 ttctttgact ccttctgttt tattcaattt agccaaagct ttttcaattt cttctcgtgc
     6361 atctgaacta ttcgcagcat aagttcctag taattgatca atataagttg aatcaatatc
     6421 atacaactta gtggtatttt cactataaaa ttcgatcgtt gaaaaatctt ctccttcttt
     6481 ttctggttct tcctctatta atgaaccctt cacggtctcg tacacgccaa tttgttcttc
     6541 caaaagttga atttgattgt tcaaagttgg agatttcacc atgtcatcat tgtaatcgtc
     6601 ataagtcact aacgattcat aagcacgatt caattcttga aatgctttga cgtactcgat
     6661 acggatttca agtggcgtat gttcatcaat ggattccatg gatgaaacct cttttaactt
     6721 accatatgct tgtttgaatc tttttttgga ttcctgataa cttggataaa tcaaacctga
     6781 ttcttctttt gcttttgaat acagttttgt cgcatcctcg acattcttct ccattgtcgc
     6841 tggcttacga aacgtcacaa tcaatccttt tgtcttatca ggatacgtac gattcgtacg
     6901 tgaaaaagct tggatcaatc ctgcatactc tagattacga tccacaaata gggtttgaat
     6961 cgtaggggcg tcaaaacctg ttaatagacg atccactaca atcgctaaat cgatttgttt
     7021 cccaaattgt ttgaattccg ctctttttcg tgccaaacga ttattgatgt ctccgttata
     7081 acgctcaatg tctgctaatg accaggaagt atcataatac tgattatatt cctcaataat
     7141 tttttgcatt tcttcttgtt gggcacttga atctcggcta ttttcttcta atgaataggt
     7201 aatagcgatt cgtggaaaat caggatcctc catcgttcgt ccttcacgaa tgagctgatt
     7261 gggaaactct tttgttaacc aatcaggctc tttcgtcatc tctttgatgg cccgataata
     7321 ctttttggcc atgtcaatgg aactcgtggt taagattgca gatttcgttg gcctgccatt
     7381 tcgaaaatca aattttgtat aggcattatc tggacggaac attttatgaa tgactttttg
     7441 gatatgttca tctgcttcaa aaacagctgg gtccaaataa ctttcttttt tgacaggttc
     7501 catttgatca atcatacgat tgatctgttc tgatgaatac tctgcatatg tttctacttc
     7561 ttttaatttc cgatagattt tattctctag tgaagtcggc tccacggtgt tttcatgctc
     7621 tacttgaaat cctaagacag agccatcttc tagcgcattc ttaatggtat aggtatgcaa
     7681 gacctctcca tactgatcat gggttgtacg tgccaattgc cctttggctt gtttccgatt
     7741 ctcaggaaaa atcggtgtac ctgtaaagcc aaaccaagtg gatttcggaa acattttttt
     7801 gatttcttcc atattttcag cacttaacgc tcgatgacac tcatctacta caaacacgat
     7861 atgttggccc attaattttt ggaagcgatt ggttcctttt ttctcttctt gtttctttgc
     7921 atatttcaac gccgcatcta gtttttgacg ggttgtaatg attacaacat ttgcattggc
     7981 atctgccaac agggtttcac ttaattcttt ggcacttccc gttccaacga tcaagctatt
     8041 cgctttggcg ttcccggaag aaatcccagt attaaattcc gaagcaaatt ttgtaaactc
     8101 tgtggtggtt tgattatcta aatctttgcg gtccactaac atgatcgttc gatcgatccc
     8161 cgattttcgt gctaaaagct tagtagatac aaaactagtc aacgtctttc ctgaaccagt
     8221 cgcatgccac acataccctg attgatgttt atttgcggca ataaacaagg cttgaatcgc
     8281 atgtacttga taaggatgta acaccatgag ggttttatta tcttgatctt cactgacgat
     8341 tgtataatcc gcaatcaaac gatgagcatc cgggatattt aacacttgtt tacaaaactc
     8401 atataagttt tctacttttt cattgtcttt cgttcgccaa ctaaataaaa acttagggtg
     8461 caagtctttc ggtaaggcat ttgcaaaata ccgagtggtt tgttcattgg aaaccacaaa
     8521 caactgtaag gtcgaaaaga tattgttccg aaacatacct tcttctgcat attttttgat
     8581 ttggttaaat gcctgataaa caccatcttt tgcagttact tgtttcagct cgatttggac
     8641 aatcggtaat ccattgatta ataaggtgac gtcaaaacgt cgatcccgtg cttctatgtt
     8701 acttccacgt tttgcaattt gatggaccac ttcataagta gagattcctc caccaatgtc
     8761 ttgattggaa taaaggataa gtgagacaga acccaattgc gaatcttccc gttcaatcgt
     8821 gatccgagcc atcccatttt caccttttaa ccatttcgca gcatcaaatg gggttttcgt
     8881 tcgcagcagt aattccgttt tgatcgtttc aaattcttta tcggtcaaag gaaaatcatt
     8941 cagttctgct tgattatttg agataatttt ttggcgtaaa ttgacccaaa ggtcttcttc
     9001 tgattttaga tccggacgat acgtccactg attatgccct tctccaagta cacgaatcaa
     9061 ctgttcttcg acttctgctt cctcacggtg actaattttc attcttcttc cctcctagac
     9121 aaacatcttt tgtaagaagc tttttttcgt ttcttttagt aaatctaact tacgttgatg
     9181 aagagcgatc gtatcatcta gctgtttgaa gaaagtaccg attttttgtt gttcatcctt
     9241 tacaggaacc ttaacactga tattctcaat atcagaactg ttataagaag gcaatgcccc
     9301 tacttgggag tatcttggca aatttatagt ttcaaacaat attttaccaa aatagatatc
     9361 ccaagtatta ttaaataaat aagccatggt attgttatct attaagaaag ggcttgtgac
     9421 caaacgtttt ctattaagca ttattgctgc gccaacttta gcaaatataa tcgctggcac
     9481 atcttttatt ggcttccatt ttttacgctc aatttgctca ttagatacat aatgatttgc
     9541 atttctcatt tcatactcat taccaatgtt attcatgtcg gaaactttaa aaaatggagt
     9601 accttcagaa ccaccttgct cggcatcagg aaatccaatt cccgattgag ttttacctaa
     9661 ttctcccaac ttacgctctt cccaatcttc cgtaaaacca ggaaaccgga tttctggcac
     9721 ttttgctccg ttttcaggga acattttttg taagaatcct tttttcgttt ccttcagcaa
     9781 atctaactta cgttgatgaa gagcgatagt gtcgtcgagt tttttgaaga atatgccgat
     9841 tttttgttgt tcttttaaat cattgggaat atagaatact gtatctgaaa cttttttcca
     9901 gtctgaacga ggcatttttg ttccagatgt atcatttgca gccttttgat atctatcagc
     9961 ttgaataaga gaataaataa agtccgagtc gctcttctta gctctaaaaa cccagaaatc
    10021 cccaatcgct acgcctgtaa aagttgcctt gagccaattt tttaaatatg gacgtaactt
    10081 tccatataga atatcatttg gtaaaaattc aatccctttt ctattatcaa atttctggct
    10141 cacatcttta ttcagtctac cttctcctgc cacgatatct tcaaattcaa cttttggaag
    10201 tctactggag tttgtacttt tattaagtct aacaactaaa tcaattaact tacgctcttc
    10261 ccaatcttcc gtaaatcctg ggaaacggat ttctggtcgt gtatcattgc tcatgtttga
    10321 acacctccag cgcaccttgt aaccacattt cgttttcagg tgttgtttgc agggaagaga
    10381 tagcttctaa aagttctttt tctagcactt gctgttcttg tcgtatcttc ttgatgtcat
    10441 ttccgacatg aaccatatca attggttctt cttcttcgaa agtatctaca tagcgtggga
    10501 tatttaagtt atagtcattt tctttgattt catcaaaggt tgccaaatga gcgtatttct
    10561 cgacatcttt tctttcagcg taggtttcga ggattttttg aatgttttct tccgatagtt
    10621 tattttgatt tttcccttta acaaattcac gactagcatc aatgaataat acatctcgag
    10681 tttgacgatt cttcttcaac acaatcaccg tcgttggaat cgatgtccca aagaacaagt
    10741 tcgcaggcat accgatcaca gcataaatac tgccatcttc tagtaatttt tgacggatga
    10801 ccccttctgc agcgccacgg aataaaactc cgtgtggtaa gacgatggcc atggtacctg
    10861 tttccttcaa atgatagaac ccatgaagaa gaaacgcaaa atctgctttg gattttggcg
    10921 ctaatttccc ataacgatta aaccgagaat catctaagaa agtcgtatct gctgaccaat
    10981 tagccgaata aggtgggttc attactactg catcaaatgt gtaaggttca tctgttggcc
    11041 agtccttatt caacgtatcc ccattgcgga gattcatttc ttcggcgtcg acaccatgta
    11101 aaatcaagtt catttttgct agattgtatg tcgttgtatt tagttcttgc ccatgatatt
    11161 taacgttatc tgggtgtgtt aagtagttac gtacgtttaa catcaatgaa cctgaaccca
    11221 tcgttggatc aaaaacacta aagaatcgtc gttccttttg atcgagcgtt acgatttgcg
    11281 ccatcatatc agaaaccata tgaggtgtgt aaaattctcc ggctttcttc cctgcttctg
    11341 aagcaaattg actgatcaga aactcatacg catccccgat cacgtcccca tcgtgttcta
    11401 aaacatctac atcattcaat ttcttgatga cttcggtaat cgtcacattt cgttgttgct
    11461 catctgttcc tagttttttt gattggagat ccacatcatc aaataaccca ttaaattgat
    11521 tgtaggtaga tgccaattgc acaaaggctt tgtttagatc gttcaattgg aacgttgctt
    11581 gttttgcttg atccgctaag acgttgaaaa gatactctgg tgcaattgca tatcccaaaa
    11641 tatcaacgat tgtggcaatc agatcttctt ttgattcttc atctgaaagt aattcacgat
    11701 acaacattgt ttgtttgctt actgtgtcat attcctctaa ggattcatct gctaaaagaa
    11761 ccacttgttc caacaatcga tcggataaat atttatagaa aatcagtccc aataaataat
    11821 ttttatattc tgacgcatcc attttactac ggagattatc tgccgcacta aataattttt
    11881 gatttaattc tgcacccaaa aagatacact cctatctctt atttaaagtt gctatatacc
    11941 ctcttatttt agcatacccc cttttataaa aagcaaaaaa aaaagaaaga aacttattct
    12001 tgctaataaa ctacgcgaaa ggagtctaac gttgtttgat ataacttaga ctgatcgtta
    12061 acttattttt tatatttcta catagttatc tttagaacca tttcagatac atatgaaaca
    12121 attacatatg attttttcat aaaaaaacag tactgtctag tagtataaaa tatacaaatt
    12181 tcttaaaaat tttttcacta aaatcactac ctgaaacagt ctaaaacact tatatatcaa
    12241 cgtttaatga aactatctcc gtttcatttc agatacaaat aaatatagtc gggctccaaa
    12301 tccgaaaaaa aataccccca gtagcttatc ctaagctact ggagatgtat aagtacggtc
    12361 atataacaac ggggtagggt aaataaatga aaaagatgga agaatcatta ttcaataact
    12421 tttttaaata cttcgttgta atcagttacc tgtccgttct taaataccac agaataatct
    12481 ttttacccat tttcaagtaa ccgaagtgga atgtctttac caacttgctc tgtcactgca
    12541 ttactgatac ctgttagatt agctaataaa tctgataaag attgagcatc tgtagaaatc
    12601 tctgtatcag tatttatcgt gattccttta tcttcattta caagcttcca agtaggtggt
    12661 atagctgtgt atgtttcgat gaatgaatca attctttcat ctatcgtctt cccagtatct
    12721 acttctgtct cattactctt agatgaatcg attctttcat ccatcgtctg ctccgtaact
    12781 gcttctgtct cattattctc accggaaact ctcttcacac ccaaacttat atattgtcca
    12841 tctttagtgt gcatagttaa aattcccacg cttttactaa taaattcact ataaccagtt
    12901 ctttcaatag agacttcaat gtatttcaca ctatcatact caaaagaaaa atagacatgt
    12961 aaaagcattt tgttgtttac atctacataa taatcctttg actctacttg agaccttcct
    13021 atacggaata cagctttact gtctgatatc tctataccag catccttatt attaatctgc
    13081 caatctccaa gtagctcttc tggcaagcta gtagtttctt gtgaacactt taccacttca
    13141 ctggtagagg tcgaaacatt cacctccgca ctgttagaag aattttccaa ggaatcagga
    13201 gccttagcac aaccaacaag tctttatata ttgtgcagcc gatattttct tcattcagta
    13261 cacccttgaa atatgcagtg ttagataaag cagttcgtcc tgatttgctg aggcacccat
    13321 ctcttcttga acgtactcct tgattttcaa ggccgcccga tactctttcg gaaaacgctg
    13381 cttcactgaa gccagcaaaa aatcatcctt ctccggcaac tcttccttct taatcattcg
    13441 ggcaataaaa aactgtaaat gcgtcacaaa tcttaagtaa agcatcgaat ctttatcaac
    13501 ctttttttcg ggaaacagat atcctagcat agccaagata tcctttagtg ctcttaccga
    13561 gagcatggtt tcttccatat ttgcttgttc actttgggca tttacaatgt gaaacgcaat
    13621 gtttccagcc tcctcttctg gcaactgaac attcattttt ttgttgatca attggatagc
    13681 atagcagccg atctcaaatt cttttgggta atacttttga atttcaatca gcaggcgatt
    13741 ttgaaaaaca atgccttttt gactgcgttc aatggcatgc ttcaagtgat caaccaaggt
    13801 aaaaaataga cttttattca gcttaaagtc aaacttggcg ttggcataat cgataattcg
    13861 cttgacgcaa ataattagat catccggagc agcctcaaac agtttaatgt aatccttttt
    13921 tagagcatca gaaagtgaat ctaatttaaa tactttttgg attttgtcag ccggaaccaa
    13981 atcgcctttc tttagttgaa atccaatccc tttccccatc aaaattactt ctttgccttg
    14041 cttatctaag caaagaatta aactgttgtt taacgtttta attactctca ttagattcac
    14101 cgttctctct aagctaagcc atttcttgcg attacttttt gataccaaaa gaagctttct
    14161 ttttttattc gttttaattc tttaagattc tcatcggttc ggtcaacata tataaaacca
    14221 taacgcttat tgaagccttg atgggtacta acaacatcaa tcgctgacca aggtaaataa
    14281 ccaaaaacat ctacaccatc tgttaaagct gcttgaatag ccagaatatg tttggctaga
    14341 taatcaattc gatcctggtc attaattcgt ctatcttcct caactatgtc gggtgcccca
    14401 tagccatttt cagtaattaa aataggtaaa tcatagcgat cgtacaattt cctcaatgtt
    14461 aatcgtagtc cagtagggtc aactacccag ccatatggcg ttttatctac ataaggattt
    14521 tcagctgccc ggtagacacc ttcttctccc aacatgatct gttgatcccc cgctctagcg
    14581 gaaacatctg atccgtcatt tttgctggca gcgatggtgg cagttgaata ataattaatc
    14641 gcaataaaat cgggtttacc ggaggataaa attgcctgat cctctgcctc aattttagga
    14701 tataatccac gatcattcaa atagttttca aacaaataat tgtattttcc ccgaactgct
    14761 acatctaaga aagaccagcc tctcaatgtt tcccaattat gggctgcaat tgcatcttct
    14821 ggactggagg tagcttgata catagccgtt aaattgattg ctggaccgat ttttccttta
    14881 agattcaact gatgatacaa gtgaattact ctagcttgag ctaataaaac atggtgatta
    14941 atttgaaaga gttctttttt tgaaggcagc tctccacttg gtggcaaacc aatcgctcca
    15001 ggatgaagga tcatcgtgtt ttgctcatta attgtcagcc aatagttcac cttgtcacca
    15061 tactcagtaa gcaatacctg tacatagtcc acaaaataat cgatagttgc tcgatttaac
    15121 caaccgccct gctcctctag aaaataaggt aaatcgaaat ggtacatggt gacaatcggt
    15181 tcgattccct gattcaggca ttcatcaatt acttgatgat aaaaagaaat tcccttttga
    15241 ttggttttat tattttcaat atccataatt cgcgtccaag cgatggaaaa gcgaaaagct
    15301 tttaagccta attctttaaa cagctgtata tcttctttat agtgatggta aaaatccgag
    15361 gcaacttcaa aattagctac cccttctgta tgctgatact tgtcaataat agacggttgt
    15421 ttcccatcct cctgataggc accctctgcc tggtaggcac taatggaggc accccataaa
    15481 aacttctctg gaaatgctga aagctgtttg tattgcacaa aaatcctctc cttaactaat
    15541 ttgcatcaat ggttcgtgtt cctttaagct tccgcttgct tcactaattc ctgcgtaatt
    15601 ttcactgttg gtcactacca caatcactgt atcatcgtag ccagcgtttc taatggcagc
    15661 gaaatcggcg ttgattaata attgtcctgc tgacacctga tctcctgctt ttacaaatac
    15721 ttcaaagggt tcccctttta aattcacggt gtcgatcccc acatggatca ataattcaat
    15781 accttcttta gaacgtagac caatcgcatg tttagattct aaaatagtaa tgacttctcc
    15841 ctcaacaggt gaataaatgc tgttttcttc tggatgaatg gctacgccct ttcccaatag
    15901 tcccttagaa aagacagcat cgttcacttc agaaatagga cacacggttc cttttaccgg
    15961 attttcaaca atgactgctt cggctattgc cgtttgctct gattcatcag aagtatcgtc
    16021 aaatccaatt agataagcaa tgataaaggt tgcaacaaag gcgattacac acgtaataat
    16081 tgcgtgaaca atattcattg gattttctcc aataaaggct ggtaaggctg ctaagccagg
    16141 ggaaacaaag gcatatctga ctaaaccgct caagccagca tacaaaccag caattgcacc
    16201 accaatcatg gaagcaacta atggtttttt cagcttcaag gttactccat agagtgaagg
    16261 ctcagtaatt cccattaaag cagtgatccc agtggacaca gctagctgct tcaactcttt
    16321 gtttttagtt ttcgcggcta cagcaaaggt agcacctgat tgagcaatat tagaagccag
    16381 cattcctggc ccattgatca tttcagaacc cgtactgctc aattgcatag tagcgattgg
    16441 cgtcattgcc caagctgtac ccgtaaccac caaaaatggc tgcaaaccac ccatcaaaaa
    16501 tggaattaac caagctgctt tgccgttaat ccattcagcc cccattgcta taagatcgtt
    16561 cagcaacgtt ccaaacggtc caatgacaac caacgctaaa ggccccataa ttaaaaatac
    16621 cagcatcggc ttagtaaaga atttaataaa cgaaggcgaa attcgatccg caaagggctc
    16681 tacataggac atgatccaaa cagtaaagat aatcggtaaa actgatcccg cataatctgc
    16741 agctaatacc ggaattccta gaaaagaaac ggcatcgcca gtagaaaaaa gctgtgaaat
    16801 atttggatga agcaaaatgc cagcaacagt aatcgccaaa attgggttga catcaaactt
    16861 tatggctgcc ccataggcta atagtactgg cataaaataa aaggctgcat cagcaatagt
    16921 attcaaaatg atgtaagttt gactactgtc tgatagtaca ccagtcaaag tcaagacggc
    16981 caaaatggct tttaccatcc cggcacctgt tatagctgga atcatcggtg taaaggtggt
    17041 agaaatgaca ctaatcaatc ttgaaataat actttcttga ttgtctgaag aggggccctt
    17101 tttaattgaa gttcccactt ccttttgaat ttccttgaag gctgtttgga cttcattgcc
    17161 aatcaccact tggtattgtc caccgctatc caccacacta attacaccaa gtgttttttc
    17221 taaggctttc gtatcggctt tccttttatc tttgagttca aaacgcagtc tggtggcaca
    17281 atgggttaag ctgaccacat tttcttttcc cccaacctgt tgcaatacat ttcttcctac
    17341 ttctctgtaa tccattattc tctctcctta aagcagtaaa aaagaccaag tcaaaccacg
    17401 ccccaatcaa cattgaagtg ttagtttgtc ttggtcttgc ctgcataatc agtaacatgc
    17461 caataacgtt attttgctgt aatccaataa tagcacatta tgatagcgca atcaacaggg
    17521 aatttaattt tttatctttt cgtttgaccg tatagggtaa tataacagtt agatcacatc
    17581 aactagggta tatctgcgta tacttactgc taaaataagt atatctaata ttaactatac
    17641 cagtaaaacc attcctacat aaccagcggg aaattcccaa aatcgtgaaa gcatcaaaaa
    17701 tgcatataac ggatcacaca acttcaacaa ttctgctctt gggaaaaatt gatgaatttg
    17761 gtgagtaaag gatggaataa acagcattcc aacaatatag gcaacaatag tgatacttgt
    17821 ttttttggta aaaatcagta agcaatagcc aatttcaatc attccgagat ataaaggtat
    17881 caatagaatg atttgggcta gtattcttaa aaattaacta gcaaatgttt aaactgattt
    17941 atttctaatc tacatgtagt aagtatttca attccaccaa ttatcaataa aaataccaaa
    18001 catatccata ataatttttt ctttttcatt tcttttatat tgttataaca gtgaataaca
    18061 taaaaattcc cgacaatcat tgtaatattt ttttgtcacc tgtattagta tttaatttat
    18121 aggaaactat aaaaactccc tagtgaattt acacctttgg gagttcctgt ttggacggta
    18181 tgcataatac atttaaatca atttaacatt aacacctcag catatggtat ctccacatat
    18241 atacggtctt tctttggata gtactcaata tttatgttga tagcacttcc attgtctaat
    18301 atagctttag cccggtatgt gttatctcca ttttcatgag taatcgtcac atcatcacat
    18361 gatcgattta agttgtactg ttcttgtaaa atctgtgtta ccaatggttt tgatgacttc
    18421 tctaatccag atgtacctgt catttttgaa aaaataatac ctataatagc caagacaaca
    18481 atacctataa tgacaaactt cttgttcata tttactttat tcaacttttt atctcctatc
    18541 tccaaaataa tttgttctta tccctcttat cagtacattg acatccctaa aagtacacca
    18601 aagattgctc cagctacccc tgctatcatc aaaatcgtac ccattttctc atctttttct
    18661 cgatataagt agcccataat aacagcaaca gcaccaaata caacaggaat aaatgctaac
    18721 gatactacca tagaaatcca accaataatc aaatacagct tccctgtctc ttgtccatgt
    18781 acgatttgtt ccttcatttc attaattgtc tgctcagtac ttttctttac tgtgtctgtt
    18841 ccggttgtct catctgtttc ttgtagtgct ccacaaaact ggcaatactt actttcatct
    18901 gttatttctt tgccacattt tctacaatac atgtcttctc ctcctaaaat cattcaattg
    18961 cttattagtt aactgtttac tttattaaat acatcagaaa acagctcatt cattggtaca
    19021 cttaatatct cagaaagcat cttcatttga tagggtttag gaatcgttct ccctgtttgc
    19081 cacgaactta cacattgctg tgtaacattc attttttttg ctaactccgt ttgagaaagt
    19141 cctttttctt ttctgtatcg gctgagatta cttacttttt tcatgattct cccctcctgt
    19201 tttattatat acaacaatat gatgtatttc aactcataaa atataaaaaa cttcaaatac
    19261 cattacaaat acaacaactt gttgtactat actgagatgg gggtgtatta atgaaacaga
    19321 aacaatatgc aaaggtcatt agagaaaagc gaaaagctct cgggtggaca caaaaagaat
    19381 tagctgaaaa aatcttctct acacaacagg cagtagcaag atgggaaaat tctgtaacag
    19441 agcctaacct cgatagctta acagctcttt ctagagccct aggaacccct gtaagccatt
    19501 ttctagacaa tgttgtcgta gattatgagg aagagttttt ggctctctat cgttctctta
    19561 gcactgaaga tgcagttcga acaattgact atatgaagct attaaaaagg caagaaaatg
    19621 aacgcaatca gctattgaaa gattgaaaaa taaataaccc accgaacttt aacggtgggt
    19681 gtattgagat gaaataagat taagagctat ggataacctg tctagtttaa tacaattggg
    19741 tcttttctct tccttactct ccgtccatca tctcttgtta cacttgtaaa ttgatacaga
    19801 ggaactatcc cagctcctga taatatcaat tcttttattg tttcatctat ccctgcccct
    19861 ttcgctaaat atattaaaaa actactactt gctttcacat tgtatagttt cttaatatcc
    19921 ttagaagctc gttcaagagt gaatccttgt ttaacaaagt agcctctgtt ttttattaaa
    19981 gcatttttca cgctgttatc cccaacatta cgtaggcgtt cacaaacagt gttaataagt
    20041 tctttctctg tcattagata tgtcccttgc cacgataagc cctgtaaaac ttcatgtgct
    20101 tcatctaaaa tcaattgttt ttccttgctg agttctgatc gggaattttg aggaaatact
    20161 cttttagcct ctttttcgtt aaaacatatc ttaacccatt cataagaaaa atattgatat
    20221 gtaaaatgaa ttttttccag aagtggaagg atatcttcat tcaatttttg aatcacagaa
    20281 gttgcattaa aaacgtctaa tcgatagaca ttgcttctct tatgattaaa gtttcctaac
    20341 gctttctctt cctttaacct atttaggtat tcgagcatct tcaaaggaac atcttcatca
    20401 tctaattttt ttatcaatcc tataaaagtc ataagtgata gtaacttatt acatttatct
    20461 atgtcataag ttttaccata gtaagaactc atttttttag ctaattgttc ccccgacaaa
    20521 atacaagtca cactttcctt actatcatca aaaaaattaa catcagaaag caagacatca
    20581 tagacacgtc gcatttcctc tagataattc cttattgtcc tgaaaagttt gtctttcatt
    20641 gattcaagag tgtcaaaaag tacttttgat tcctctttca attttcgaaa atgtgtagga
    20701 tacaagtcta ggttctctaa tagccaatta aatgtttcta cccgactgtc tttctttaaa
    20761 attagcatca caactccaat aaaattttca ctgatatcac aattactgga atgacaataa
    20821 aaaatggaat gcccagtttt actggtaaaa atactagcag agggattatg atcgttgtga
    20881 aacagacatc ttaagttttt acttgactta agtaatttat tcatatcaat ggttaacaat
    20941 ctttcataaa tctcattaaa agacatgtcg gaggaatcaa acaggcagtc tttaaaccac
    21001 tgtttcattt cttctttgtc gtcatttttt atcaacttaa caaaatcggt ttcattgact
    21061 agttcatact ctctccctga atcactttga caatttttag aacatgtcat ggtaaaatca
    21121 atcggaagct cagtaatatc taacatgttg tcttcattaa ttattatcac tgcatctgac
    21181 ttatatcctc caaaaaacat tctattcgaa gcatttacat tgtcatcaca cccggggatg
    21241 ttctttttca agtattcgta aacagcaacc attttaccat agtcagtaat tgcttctttt
    21301 aatagaaaga caatcttgta tttttctaga ttttcagagt accgaatact tttttgtatc
    21361 gcaaacgcag aattatgaac taaattgata gataaaacct tttctatgct taagtatcta
    21421 ctatcatctt tactcaaatc tactaccttc cccttctcat ctttaacagt gttatcgaca
    21481 tccagaataa ttactctggt ttccacaaaa ctctccttgt cattgccttt attctttgtc
    21541 ttgcttggaa taaatgattg tgatgaattt agtatctcta caaactcatc tcttgtaact
    21601 tctctatagt tatctaaaca cctatctctt acatcagttt tttctttttt tgtaactggc
    21661 ttctctaata atttttcttg atctacataa attggaatat taatcattga tgtttccatt
    21721 tgtgtttcct ccttataaaa ttctgttaaa gactaaaaca tacagataat aaaaacaaac
    21781 tctttattac tatatgtttt agtcttattt gtttactcaa cctttatacc gttccatgaa
    21841 actatcaata tcagattttt taaatctaat gaccgtatca atttttataa caggtaaccc
    21901 gtattgagaa acatattttc taatcgttcc tactgataca cctagatatt cacttgcctg
    21961 ctgatagtcc atatactcta gagtgactcc agaaataatg tcttctagca taggctttag
    22021 cactgtaatt atctctttca cttgttgttc aacttgtata gaaacaatgc tatcaatgat
    22081 ttgtagagac atatttcccc ctcctttcgt gattgcttga acaaattttc taagaattat
    22141 cttcgtattt tttgctcaaa ataaaagtat tttgttatta accgattaat caaacaaaaa
    22201 atgattacca gttcatcgac atacacataa tacctcgata aaaaaggggt gtcaacagct
    22261 ttgtaaaatt tatttacaac accccttttt tgaggtatac taattttagg aggtgtggtc
    22321 aatatgataa aacgtccacc aataaattat ttagaacgaa aaaagatact cggaacaaaa
    22381 ataaaagcta taagaaagag taaaaaatta acacaaccag catttggtct tatgattaac
    22441 aacggtcaat taattgacaa aaaaactatc tacgaatggg agaagggaac ctatttacca
    22501 ataccagagc gattatcccg aattgctgat ttgggaaata tgagtataga ggagttagtt
    22561 tgtggcaatg tagaagaata tattttggga attattcttt atagagatag cattgtacta
    22621 gatggtataa catttccaga taaaaacctg ttccagcatc ttcgtcaaca gttcccacca
    22681 gtacactcta atttagatac atggctggat agatattcca aattagaacc agagatgcaa
    22741 gaatttattg ctaacaagac ttgtaataaa gttaaaaatg aaaaaattag tcttttcaat
    22801 atcttaaaaa tagaagaatt attcatcaat gctattgtag aagaatttga caataacatt
    22861 cttttcctca ctagcagtat agaagaacta ctagaaagaa tggtagatga atggctacct
    22921 attcaattga aagacatgtc ttatcccgaa gaagctgtga gagaaattac tgataatata
    22981 aacaagctag aacaaacgat ttcatcaatt gggaaaaaat acactaagaa aaaaatgaag
    23041 ggaggtgata ccatctagtg gtaagtactc cttgtaaact aagaggagat gatttaatgg
    23101 caaaaatatc aaagtacaaa aagaaaaacg gtcaagtggc gtggatgtat aaaggacaca
    23161 ttgccaccga tccacgaact ggtgaaaaaa taaatactac gagaagaggt tttagtagca
    23221 aacaagaagc tcaaaaagat tatgacgagt acagacatca aatattatac ggtgttaaaa
    23281 agaaagcacc tgatatgaca tttgaagacc tgtataatga atggataact catcagcgta
    23341 ccagtgttaa agcttcaaca atcgcaatct cagtaagata cgcaaataat caaatacttc
    23401 cagcatttgg taagcttaaa ctatcgaata tctctgtccc atattgtcaa aaagtggtag
    23461 atgaatggca cagtaaatat gaaagttacg attatatgag aaaacagact gcacaaattt
    23521 tacgctacgg ggttgcaatg caatatattg acaataatcc tatggaaaaa acacttcttc
    23581 cacgaaaaaa agagtatgag aaaaatcgta aattttactc aaaagaggag cttaataatt
    23641 tactggatgc ttttaaagat tttggtaaca tgaaacaata cgccttcttc agattattgg
    23701 catacacagg tatgagaaag agtgaagtac tcgctttaca gtggaaagac atagacacgt
    23761 ttaataaaga actacatgtc aataaaactc ttgctgtaga tgaatttggt aaagttataa
    23821 ttcaatctcc gaaaacaaga gcttctagac gagttatctc tttagatact gagactcttt
    23881 ctatcctcaa caactggaaa ttacagcaaa aagaagagta tttaaaactt ggatacaata
    23941 ctagctctaa agagcaacat gtttttacaa cagttaaaaa cactctatac attccaaaca
    24001 ctgttaatga ttggttaaga tatattttga aaaaatataa cttgcctcgt attacacctc
    24061 atgggtttag acatacacat gctagtttat tattagaagc aggagaatct gtcaaggtag
    24121 ttcaacaacg attagggcat gaaaatagta aagtgacatt agacatctat gcacatatta
    24181 caaataatgc acctaagaaa acaggtcaag actttgctga tatgatggct catcagtaaa
    24241 agagctggtc aaaatctggt catttctagt atacaggcaa aacaaaaaag ctagaaccct
    24301 tatatatcaa gggttctagc tcctataaca ggtttaccca acactgcctt ccatctcata
    24361 actgatcaat cgattgagtt cgactgcata ttccattgga agttctttgg tgaatggttc
    24421 aacaaagccc atgacgatca tttcagttgc ttcagattcg gtcaggcctc ggctcatcag
    24481 atagtagagt tgttcttctg agatttttga gactttggct tcgtgttcta aagagacttg
    24541 gctgttgtgg atctcattga atgggatcgt atcggatttc gaccattcgt ccatgatgat
    24601 cgtgtcacat tcgatatgag agatcgaacc cgcactgttt ttggcaaagg ttacttgtcc
    24661 tcgatagttt acttctccgc cgtcttttga aattgattta gagacgatag agctagatgt
    24721 atttggtgca ttgtggatca tttttgcacc cgtatcttgg atttggtttg ctcctgcaaa
    24781 ggcgatggag agcatggtgc ctcgtgcacc ttttccatct aagtaaacac ttgggtattt
    24841 catcgttgtt tttgcaccta ggtttccgtc gatccattca acggttgcgc cttcgtatgc
    24901 ttttgctcgt ttggttacta agttgtacac attgtctgac cagttttgga tcgttgtgta
    24961 acggcaatac gcatcttttc ttgtaaagat ttctacgatg gcggcatgca ggctgttact
    25021 tgaataagta ggtgccgtac aaccttcgac atagtggatg cttgctcctt cgtcaacaat
    25081 gatcagcgta cgttcgaatt gccccgtgtt ttccgcattg atccggaagt acgtttgtaa
    25141 tggaacatcg actttgacgc cttttggcac gtaaataaag gtcccacctg accatacggc
    25201 tgaattcagc gctgctaatt tgttgtcggt tggcggtaca agttttgaaa agtactcttt
    25261 gaataattca gggtattctt tcaaagcaga gtcagtgtca gtaaagacga ttcctaactt
    25321 ttcgaattca tctttcatat tatggtacac gacttctgat tcatattgtg cggaagcacc
    25381 ggctagataa gctcgttcag cttctggtat accaatcttt tcaaatgtat ctttgatttt
    25441 ttctggtacg tcttcccagt cacgagcggg tttgtcactt ggtttttggt aatatttgat
    25501 ggcatcgaaa tcgatgtctg aaaggtcggg gccccatgtt tgcattggca ttttgttaaa
    25561 tgtttctaga gatttcagac ggaattcaag catccattct ggttcgcctt tgattcttga
    25621 catctcacgg atcacttctt ctgtcaagcc ttctcctgta ctgaaaaccg gttcgacatc
    25681 atcgtgaaac ccgaatttat attcttctaa ttctggtacg cccatgttct cactcctctt
    25741 ccttgtggta atgatggaaa gctgcggtac catcgttttc cattgctttt cctagtgctt
    25801 tccaggcaag tgtcgcacat ttgatccgag cgggaaattt agctacacca ccaagcatag
    25861 cagcatcgcc taattggtcg ggatctttga cttcttttcc ttggacaagc tgtagaaagt
    25921 ctacagttaa atgttctgct tcttctattg attttccgat gacggcgtct gtcatcatcg
    25981 aggcacttgc cgtactgatg gagcagccac tgccgtcaaa ggcgatattt ttgatgattc
    26041 cgtcttttac atcgacttgc aattcgatca catctccaca tgtggggttg ttcaattcga
    26101 tcttgcttgt cgagtcagtc aagcttccac gatgatgcgg atgggaagaa tgatccaaga
    26161 tcacttgacg gtataaattg tctaatttag atagtcccat gttggaaaaa ctcctttgtt
    26221 gccatgattg cttcaactaa acggtcggcg tcttgcgttg tgttgtagaa ataaaagctc
    26281 gcgcgagcag tcgctgatac ttcaaggtac ttcaatagcg gttgcgcaca atgatgacct
    26341 gctctgacgg ctaccccttc catgtctaaa gctgtcgcga catcatgggg atgtaaccca
    26401 tccaaattga atgcaacgac tcctgtccga aaattcgggt cttgtggacc ataaacagtc
    26461 aatccatcaa ttgcttgtaa ttttggcaac acataagcgg ctagttcttc ttcatatcga
    26521 tggatattgt ccataccgag tttttgcaaa taatcgattg catggcctaa tgcgatagcg
    26581 ccggcgatat ttggtgtccc tgcttcgaat ttccaaggta gttctttcca tgtactgtcg
    26641 tataattcga caaaatcgat catttcgcca ccaaattcaa caggttccat ttcttctaac
    26701 cattttcgtt taccatataa aacaccaata cctgttggtc cgcacatttt gtgtccgctg
    26761 aaagcaaaga aatcacagtc tagtgcttgg acatcaatgg ccatgtgcgg ggcagattga
    26821 gcaccatcta ctacgatgac cccgccgttt tcatggacaa gttcagctaa ttcttgaaca
    26881 ggattgaaga cacctaagac attggataca tgagcaatgg agacgatttt ggttttgtcc
    26941 gtaatctttt tcgctgcatc ctccatatcc aggaagcctt cttctgttag ttcgatatat
    27001 ttcaagacag cacctgttcg ttttgccaac tgctgccacg ggataacatt agaatgatgt
    27061 tccatgtaag agagcactat ctcgtctcct gctttgatat agcggtcgcc gaaggttctg
    27121 gctacccaat tcaagcttgt cgtcgtacca cgagtaaaca gcacttctgc tgtttctttt
    27181 gcatggatga acgcccgtac tttttctctt gcttgttcat aatcatgggt cgcgcgttct
    27241 gctaacgtat ggacaccgcg atgaacattg gcgttatcgt gttcgtaata gtacgctaat
    27301 gcctggagta cttgttttgg cttttgggtc gtcgctgcat tatccagata aactaaaggt
    27361 tcatcattga cgtcttgaaa taagatagga aaatcattgc gtagcttttt agcatcgatc
    27421 atccattcaa cttcccttcg attacttcga ctaattcttt ccgaacttgt tctacaggga
    27481 ttgctgttaa gacagaaccg aggaatcctc gaatcactaa gcgctctgct tcttctttat
    27541 gcaatcctct actcatcaga tagtacattt cttctggatc aactcgtcct acactggcag
    27601 catgtccagc agttacttcg ttttcatcga tcaaaagaat aggattcgca tcaccacgtg
    27661 ctttatcaga aagcatcaag acacgacttt cttgttgggc atctgcacct tttgcccctt
    27721 tcaagatgtg gccgatacca ttaaaggtca atgttccttt ttctcggatc actccgtgtt
    27781 gcaaaatatg cccgatcgta tgaggggctt tattggttac tcgtgtatcg attccttgga
    27841 tttgtctgcc cgagctgatt gccacgattt tgacttctgc gtgtgcacct tctcctgcta
    27901 aatctgagtc aaagtcagcg atcacatgac cgtcattcat gacaccaatt gcccagtcga
    27961 cagaagcatc tcgtaaaatg tgcccacggc gattcatata agaagtaata ttttctccta
    28021 attgatctac ggctgaatat ttgatttttg aaccagcttt agcgatgact tccacgatga
    28081 tattacctga tgattttgct acttgttctt tggttgtctg gaaacgttcc agataagaaa
    28141 attcactatg ttcatctgcc acgatcaaca catgcttgaa gaagtgctca tcggatgcgc
    28201 catcttgaat gaataaggac tcgagtggtt catcgatcac gacattttta ggcacataca
    28261 ggaaaatacc gctgttcata aaagctacat ggagtgcagt caattggtct tcgttcatgt
    28321 ttactgcttt tttcatataa tattcttgga ctagttctgg atattcgatc attgcagtga
    28381 acaagtcggt aaaaatgact cctttttctg ctaattccac aggcagttgt tcaaaaatcg
    28441 tccatgaacc ttgttggaca agcacaggat tgtctttcat tgcatcaaat gccggaatcg
    28501 tgcctgtttc actgatggtt tgtgtttctt tcacatcaaa taacgaccag cgatggaatt
    28561 tcacccgatc gatatgcggc aatggcagtt catctgcttt cgctagtgcg tcttgacgga
    28621 aggttctcat ccaatcaggt tcgcctttac ttaaagaaaa atcatttacc gcgtcaagat
    28681 aatcgagcca gtttttttct ttcattcttg ctccccctta cgcttcttct tctttatagt
    28741 cgattcctaa ttctttactg ataccagcgt agccttctgc ttccagacgt ttagctaggt
    28801 cagcattccc tgtcatgact actcggcctt ccatcatgat gtgaacgaca tcaggtgtga
    28861 tatagttcaa taatcgttga tagtgggtga tgatcaatgc accgaaattg tctccgcgca
    28921 tttcatttac tcctttagca acgactttca aagcatcgat atccagacct gaatcgattt
    28981 cgtctaaaat tgcaaatgta ggttctaaca tcaacaattg caggatctca ttccgtttct
    29041 tttctccgcc agaaaaacct tcgttcagat aacgttcagc catttcttct ggcatattta
    29101 gtaaagccat tttttcatct aactttttca aaaatgccat aacagaaatt ttgtcgtttt
    29161 catcacgttt cgcattgatc gctgcacgca tgaattccgc attcgtgata cctggtatct
    29221 cacttggata ttgcatagct aaaaataagc caagtcgcgc acgttcatct acttccaagt
    29281 ctaaaacatt tttcccgtca aacaaaattt cgccttctgt tacttcatag tttggattgc
    29341 ccatgatagc ggcagataag gtagattttc ctgtgccgtt aggtcccatg atggcatgga
    29401 tttctccggt tttcattgtt aggtttacgc ctttaaggat ttctttttcc tcgatcgaca
    29461 catgtagatt ttttatttct aagacagaca tgcattatac ccccaaattc tctttcgttc
    29521 tcactcattt tagactaatt gagaatgaag cgcaatgacc ttccttacta aaatcaaata
    29581 tcttttattt attattaaac aattttgatt tctaaattag aataggtata aaaaagatga
    29641 gaaataagcc attacagact tatttctcat cttttttgtt ccatacctta ttttgcatca
    29701 gcctttactg gtttaacaga gccgccccat ttgtctgtga tccattcttg gacttctttg
    29761 cggtgaagca cttcaatcag ttttttgatt ttgtcactgt cttcatcacc tttacgagtc
    29821 gcaataatat tcacgtaagg agagttgtcc ttttcaagca atacggcatc ttctaatgga
    29881 ttcaaccctg cgccgtaagc gaagtttgcg ttgattgcaa ctaggtcacc ttcatcattt
    29941 tggtacgtag aagctaaaag tgatgggtcg atcgtatggt tgaacttcaa gttcttaggg
    30001 ttttcttcaa tatcatcgaa tgttgctgtt tccagatcta ctccatcttt gactttgact
    30061 aagccagcat cttgtagaat tgtgatgatg cgtccccagt ctgattcaga atttgaagta
    30121 atgattgttg caccatcttt taattcggaa acatctttga cttttttaga gtacaagccc
    30181 attggttcga tgtggacagc accggcattg acaaagtcat atcctttttc tttgatttct
    30241 ttgttcaaat aaggaatatg ctggaagtaa ttggcatcaa tgtcgccttc ttccaaagat
    30301 tggttcggta atacatagtc atcgaatttg acgatttcaa gatcgattcc ttcatctttc
    30361 aataatggtt tcacatgttc caaaatctct gcgtgcggtg tcggtgaagc gccgactttc
    30421 aatgtcgtgt cgtctgcttt atttgctgaa tctttgctgt cgctgttgtt accgcatgct
    30481 gctaatccta cagtcagcaa aagcgctgct gcaaatccaa aaattttctt tttcatatcc
    30541 tacatccccc tatatcctat tatcttttgt ctgttttctt agttaagaaa tcaccgattc
    30601 cttgaacgat aaatacgatt agtaaaataa ttaaagtcgc aacgaatgtc actgttagat
    30661 taccacgctg gtagccttcc tgccaagcaa gacctccaag tccacctgcg ccgatcgcgc
    30721 cggccatcgc agtaaatccg atcatcgaga tcgttgtaac tgttaatcca gaaatcaatg
    30781 cgggaatact ttcaggtatc aagaccttcc agatgatttc ccagtaactt gctcccattg
    30841 catctgctgc ttccagtaca ccagggctga cttcacgaaa agcaatctct actagacggg
    30901 cataaaatgg tgctgcggat aaaacaagag ctggaatggc tgcttgcgca cctagcatag
    30961 tgcctacgat tgctttagta aatggaatga tcaaaaccat caggatcata aatggcgttg
    31021 accggaaaat attactgatg atcgatacga ttccataaat gattcgagca tacggtttgt
    31081 ttttgcgtcc aatagagtaa aggatcaatc ccaaaactaa tccgatgatc gtcactaaga
    31141 ccattgagat taatgtcata tatagtgtat caaggatagc ctgattcatc gtttccgggt
    31201 tgatttgttt aaaatctaga tatgtttctg aaaagctttt atccacgatg gatcacctca
    31261 attcctactc cttgttcttt gatgaacact tctgcttgat ctaattcttt ggtttcacca
    31321 gtcatcaaga cagtcaatga accaaaggaa ccttctttca cttgtttgat ctttccatac
    31381 acaacgttga catcgatgtt atagcgtcga atcgcttggg aaatgactgg ctcgttggca
    31441 tttctttcgc taaaggtcaa agttgccaaa cggccgcttg gattttcttt gatgagttca
    31501 gctaatagtt ctgtcgaatc agcttctggt tcgatgtctt gttgtacaaa gcgtttggtt
    31561 acttcttttt tcggttgtct gaagacagtc aatacgtctc cttcttctac gactttcccc
    31621 aactccatca cagcgacttt gttgcagatt ttgcggatca cattcatttc atgtgtgatc
    31681 aagacgatgg tcaaattcaa tttcttattg atcgctaaca ataagtctaa tacttcttct
    31741 gttgtttgcg ggtccaatgc gctagtagcc tcatcacaaa gcaggatctc aggatcattt
    31801 gccaatgctc gtgcgatacc gacgcgttgt ttctgtccgc cggaaagttg tgaaggatat
    31861 gcttgtccgc gtccttctaa cccgactaaa cgcaacagtt cttctgcccg ttctttacgt
    31921 ttgctctttg gtacgccagc taattccagc ggcagctgga tattttcaag tacagttctt
    31981 gaccataaca ggttgaaatg ctggaagatc ataccgattt tctttcggaa tgtacgtaat
    32041 tctttgtttt ttaattttgt aatatcttga tcgtttacga tcacttcgcc gctcgtggga
    32101 agctcaagtc cattcaggag acggactaat gtactttttc ctgcgccaga atagccgacg
    32161 attccataaa tatccccttt ttcaacgttt agtgaaacgt cattgacggc ttggatgttt
    32221 ccgtttttcc cattgaatgt ctttttgaca ttcgttagtt ggattaatga catgccattc
    32281 acatctttct cttatgattt ttcaaacaaa aaagccttcg ccctcttgcc ttaagcaaaa
    32341 ggacgaaagc ttgaacttcc gtgttaccac cttgatttac cgttacttcg caataacagc
    32401 ctcttccagt acttggactt agcttctttc cgtatactgt cgcgctgtaa cgggcactcc
    32461 cgtagtaagt ttgcttacgc tcgcttactc tgctctaaga ccatcttcca tctcattttc
    32521 gttacccatt ttcagcaaac agggctctct tcaaacgtcc gtgacatgta ctcttctttt
    32581 caaagctttc tcgtttcata taggattgct tatgattata gcaactggaa gaatcagtgt
    32641 caactttttt caagtgattt tctgatttta taattcgtca aattcttttg gatcaacatc
    32701 tttgaaactt aagatccacg cattcatttc atctgaatca ttcaagatat tgatatcttc
    32761 accgatttta tcattaacag aagaaactgt acccgttagc gggctggcaa attcattgac
    32821 cgctttttcc gcttcaactt cgatcagagg ttcatctttt tttagcgatt gtcctgcttt
    32881 tggcagattg gcaaacgtga tgtctccaag ttcttcttgc gcttcatttg tcaaaccaac
    32941 acagtattct tttccattaa ataagatcca aaggttgtcc tttttcttca aatgcttgcc
    33001 tgccatttta tatcagttct ccttcataaa ttttttcatt gaatccaatt gcaacaagct
    33061 ggtcctctcg taagatcagt ggtcgcttga tcagcatacc atctgtcgat aagacttggc
    33121 tcgcttttga agccgtaaac tcgcctactt gatctttcag tccaagttcc cgatatttca
    33181 tcccgcttgt gttgaacagg cgacggattg gtacttgact cgtttctagc caagtctcaa
    33241 tcgttgaggc cttcggtact tctttgatca tatcaacggt ttgataacta acgccatgct
    33301 ggtctaacca cgctttggct tttttgcatg ttgaacattt cgggtaccag taaaaggtgt
    33361 acaattttct tacctccttg aagatcttac aggtgcttca ttttggtatc tcatcgataa
    33421 tatcaacccg ataccgatca tgttccctaa aatcgaggat cctccctgac taatgaaagg
    33481 caatgggatc cctgttaaag gaagtaatcc gatatttgct ccaatattct caaagacatg
    33541 gaacagcagc atcatgatca aaccgcttgc gatataggcg taaaattcat tattcgtatc
    33601 gaaacacacc cgaatcatac gatagattaa aataaaatac aagaaaatga cgaatgcacc
    33661 accgataaag ccgaagtttt caccgataac ggtaaaaatc atatcggact cacgaacagg
    33721 cacatagata ttgcttacgt tgaatccttt accaaacaag ccaccggtac caattgccaa
    33781 aagtccttgg gccggctgat aactcattcc tgacgtatca tggaaagggt ccagccatga
    33841 atcaatacgt gcaaactgat aagatttgaa tccaacttta taaagcaagt ctcttccgcc
    33901 ttcagtcgta accaagaaga tcgttcctgc tccaacgact actgccaaga ttactacagg
    33961 tgcgatgatg cgccaagaga ttccagacat caggaaaatc ccgccaaaga tcgcaagaaa
    34021 gacaagcatc gttccaaagt catcttgggc catgatcaat gctaaaaccg gtattgtcac
    34081 gatcaagagc ttcccaatca gccaaaagtc cgatttcatc gtccgttctt ttacttgcgt
    34141 attatgttta gttacgatca gtgccatcat caagatgtac gcaatcttca tcaactctgc
    34201 tggctggaaa gtaaacgcac cgaatcgaaa ccagttttta gaccctgttg aagcagctaa
    34261 attctgatca tagaatttga gcaagagtcc cattaccact aaccccaaac cataaaggta
    34321 aggagtcagt ttccagatcc atttggaatt caaatgcatg atgacaacga ttgctgccgc
    34381 tccaactaaa taccagacaa cttgcatccc tacgcctttc aagactgctg gattgttcgg
    34441 atcatggcta agtgctacat ataatgagag cagaccaatc aaacataaca gaaagactgg
    34501 taaaatcacg ccataatcaa tacgattatc gttcgaagct tttaattttt tttccatgtt
    34561 tcatcccttt tcttaaaaac catactgctg attattatag cggtccagtt agataatgga
    34621 aagtctttcc atagacattt gggaaaaatt aactttccgt ttgtgcagtt gcttgagcaa
    34681 atgcagtaca aaagcctact gcttatcttt agtgtatcat agaacttttt ttgatacatg
    34741 caattatcta ccagctgaga ttctcctcgc tgtgagaagt ttctgtgctt tcattgtatc
    34801 gaaggatctc ttcggcaatc tctagcagct gatcagaaac cagattggtt cgttggatga
    34861 aataaaaata tcgatggaaa ttcaacggag tcattggcac attttctgga acagagcgct
    34921 tagacaaaat cgagcgaccg attccttcct tcagcaacgc aacaattaca tcgttacttt
    34981 tcacgatcat cttcttatct tggatattca tctcttcaaa ataacgctgc gtgtaatgaa
    35041 aaacaccaga gtttgcttct cttacaagcc acaatttgct ttttgggtcc ccagcgatga
    35101 ctagctggtc ttccatcaaa gagtatcttt gtacgccgga agtctctaat ggcttttcga
    35161 taaaaccaaa atcggattca tgtttcgcta cttgttctaa tgattcatga gaattcgtga
    35221 tttcgatctc aaaatgcact ttaggaaacc ttttgatcag atgtttcatc aactgaggca
    35281 aaagatagac agcaaaggtg tgggaagcaa cgatccgaca tacttcttct tcattttgtt
    35341 cattgagcaa tttttgctga gtttcttccc attcttctaa caaaaccagt gtccgttcat
    35401 ataataagtc ggcttgctta gttggaagca tctcttttct tccgttacgt tgaaaaagat
    35461 cgatattcaa ttcccgctcc aactgtttga tttgattcga gactgctggt tgagataaaa
    35521 acagtacttc tgctgctctg gaaaaatttc ttgtttcata tactgctcga aaagtcgtta
    35581 ataacttaaa catggcacca cttccataac tttttatgat aattactata aaaactattt
    35641 gatttaaata tacaaaaaac catctataat agaaatgtac aacaaaacag ttgctgatcc
    35701 aactagtaac ttagatgaag acaatcagct gtctgcttgc tcgtaatcat acattcctat
    35761 tcattttaca atgtattcgt tcatcttggt actaaaatct attaaataaa aaggagaata
    35821 ttatatatga agaacttttt gaaaattatt ccgatcccga tctgcgggtt gatccttggg
    35881 ttagcttccc ttggaaatct gtttaaagac tatcatcatg ttgctttagg caatctaact
    35941 ggcggtatct ctatgattct tatgatattc atttttgtta aattgtttat cttattcgaa
    36001 cacacgaaac aaacgttgca tgatccaatc atcgcctccg tttcaccaac ttttacgatg
    36061 tcactcatgg ttatttgtac ttattttgtt tcatatgaaa gtattgcacc aattgtcaaa
    36121 ttcatttggc tgtgcgctgt tatcttccaa gtgatccttg tcttttattt caattatcac
    36181 catgttatca aagctgatct ttcaattgaa gcaatttatc caagctggtt tatcatttac
    36241 gttggatttg gggtcatcac tgtaactgcc gggaacttct tcccacttat tggaaaaatt
    36301 ttcttttgga tttcattgat ctgctatgcg ttcttattgc cgatcatcat caaacgtgtc
    36361 tttttcgtaa gaaatatggc tcatccgaca cgtccgctga ttacgatcgt tgcagcacct
    36421 ggttctcttt gcctaacagg ttatcttaaa aactttgctg aacctaattt ttatcttgtc
    36481 gtagcattgt tcgctttatc tcaagtattg tatttcgttg tgctgtctat gcttccacgt
    36541 ttgttcaagc ttcgttttta tccaagctat gcagccttta cattcccgct tgttatctct
    36601 gcaacagcta tatttactac tgttcattac ttcaacagtt taggtatcta tagtagcgta
    36661 ttggatttct taaaagttgc ggaatgtctg tttgcaagtg tagttgtctt ctacgttctt
    36721 gctcattacg tacgtttctt aattaaagaa catagcaaac atggaaaatt tgtttctaaa
    36781 aaaatcgctt aataatcgat tcaataggtt gtgaaaaagc tgttctgcat caagtaataa
    36841 gaacggccaa acaaaaacag ctgttcatgt ttttgtactg gttgggctta ttaccgcaga
    36901 tgcaagcttt tgaacaccgt ttaccaagag gtcgtaaaag aagtggtcag cttcaagaaa
    36961 taagaaggaa tttacgaaaa ttgcttctca aatttttgtg aaatttcggc ttatttccgt
    37021 agaagctact tctggaacac cgtttattcg aaacaagagg tcgtacaaga cttttgtcac
    37081 gacctcttgt tcttttgtca aaatactggt ttagtgctac tatgttcgag tatgctttta
    37141 gtatttattg acgaaaggaa ccgatcaaat gaaatcattg tcacatccaa taaaatctgt
    37201 aaaggattca gcggtccgcg aaatcttacc cggtttactg ctttcttttg ccatcgctgc
    37261 cttcagcagg cttcttgcta tttatctgcc acagttaggt ggagcgacta ttgctatttt
    37321 attgggtatt ttattaggaa atactttttt taaacagcct gttctagcaa tcggcacaaa
    37381 atttgctgaa agcaaactat tagaattctc tgttttttta ctaggtgcaa ctgtcacagc
    37441 tcaaacgatt gctcaaattg gtgtaaaagg aattgttttt gtgttactgc aaatgtcagt
    37501 cactatcatc ggtgcttact tgattggtaa aaaactgcaa ttcagcgata cttcttcttt
    37561 gatgatggct ggaggaaacg ctgtttgtgg ttcttctgct attgcttcaa tcgccccttc
    37621 catccatgcg aaagaggaag aaaaaggaca gatcatcaca ctagtcaatc tactgggtac
    37681 tgtgatgatg ttgactctcc cttttcttgg attacagttg tttggtggaa atctcattga
    37741 aaaaagtgca cttttgggtg gtacactcca atctgttggt caagtagtcg ctggggcaag
    37801 cttacttgac aattctgttg tcgaattcgc catgctattt aagattctac gtattatgat
    37861 gctcgttgtt gttgtctatc tgtttgaaaa attcatgcac aagaaacaac cagctctgtt
    37921 tgaaacgcca actgatacgc agaaaaaacg aaaattaccg cttccttggt atgtattagg
    37981 gtttgtgatc ttttgtttgg tcaacagctt gttttcttta cctgtagtta ttgaccaatc
    38041 ggcacatttt atcagcagct ggtttgagat cactgcttta gcggcaattg gtctgcgttt
    38101 agactttgca aaattcatga aagaaggtac acgcttctta tcttatggat tagctgtcgg
    38161 gcttctccaa acagtgatgg ctttactatt tatttggctg atcaaagttt agaatgaaca
    38221 tgaaaaaaca gttgtgattc tcgcatggaa caatgtccga atcacaactg tttttaattt
    38281 tgcagatatc cttctaacaa caacaattgg cgtttgattt caaccccatc tattgcgttt
    38341 cccatatatc ctgtcagttt tccattttgc ccgatcactc gatgacaagg aataacgatt
    38401 ggcaatggat tggttcgatt tgcctgccca atcgctcgca cagctttcgg actatcaaca
    38461 gcaacggcta cttctttata cgttcggatt tctccataag gaatggtcga caaagctttc
    38521 cagacatttt gctgaaaagg cgtacctctc tttatagata aaggaacatc aaattctttt
    38581 ctttttcccg caaaatattc ttcaaactgt tcttgtgtgc gcgttaaaat tcgctgattc
    38641 gctgattcgc tttctgaaat tggctgaaaa gatacagcag tcaatccgga ttcatctgca
    38701 tctaaccata aaaagccgat aggtgtgtgt attttcattt tgtttgctcc tagtcaatag
    38761 actggaactt cctccttttt ctctatccaa aactacttgc caactaataa aaattgttct
    38821 taaaagtttg ataaaccgag gaaacatggt aaactaaata cgttagttta caaaaagaat
    38881 acctgttctt ttacgtaaaa tcaaatttta aaggtgttat ttaaggttta ttaaattttg
    38941 aatgtaaacc atatataaac atacgattga aaggatagaa acgatgaaag tatcaattgt
    39001 tgtcccatgt tttaatgaag aaaaaactgt tcctcttttt tttgatgagg ttgaaaaatt
    39061 caaaggtgcc catgagttcg aatatttgtt tattgacgac ggatcaaaag acggtacgtt
    39121 agcagcaatc aagaagctag cctcagctta tccttatgtc cgctttgtct cattttccag
    39181 aaattttggg aaagaagcgg ctctgtacgc tggattgcaa gcttctacag gagatttagt
    39241 gaccgtgatg gatgttgatc tgcaagatcc gcccgaactg cttcctgaga tgatcaaccg
    39301 gatcactact tctgatatcg attgtgtcgg cacccggcgt tcgactcgtg aaggcgagcc
    39361 tgcgatacgc agtttctttg cgaaaaaatt ctaccagttg atcaataaaa tcagcgatac
    39421 agaaatggtc gatggcgctc gtgatttccg cttgatgact cgtcaaatgg tggatgccgt
    39481 cctagaatta acagaataca accgattttc aaaaggatta tttagctggg tcggatttaa
    39541 aacagaatat atttcttatg aaaaccgcga acgtgtggct ggcgaaacat cttggtcctt
    39601 ctggaaatta ttcaactact cgattgatgg tatcgtgaac ttctcggatg caccgttaac
    39661 gattgcttca tttatcggcg ctttgtcttg tgtcggttct ggtattgcaa ttttgttcat
    39721 tattttgcgg gcattgttgt ttggtgatcc tacatcagga tggccttcca tggtctcgat
    39781 cttcctgttc attggtggaa tccaattgtt atgtcttgga atcatcggga aatatattgg
    39841 gaaaatcttc ttagaaacga aacagcgtcc tgtttatatc ataagagaaa cagaaaaagt
    39901 gaagccaaat gaattgaagc ctcaccaata atatacgtca aatgaagtaa acattttttt
    39961 aaatgaaaaa ggactgtgac aaaagtcttg tcacagtcct tttttccgaa taaatggtgt
    40021 tccagaagta gcttcttcgg aaataagccg aaatttcaca aaaatttgag aagcaatttt
    40081 cgtaaattcc ttcttatttc ttgaagctga ccacttctat cacaacctct ttcatatctt
    40141 attacttctt cattgctgct tttagtgctg ctgctaatgg gctttcttct ggttcttctg
    40201 cttgtgcata ttttttcagc aagcgtcgtt cttcatgttt ggtcattttc ttcttctgct
    40261 cttttttatc aagcaatttt tctgtcgttc catcgtattt acaacggaaa taagcaccgt
    40321 ttttaccttc gatgatttcc attttccggt gacattgcgg gcatcgatga ttagagactt
    40381 ttggatcttt tcttctgcgg tagttgcaat ctgaattggt gcagacatag attttgccgt
    40441 cacgtgtgtt tttctcgcgc agattggccc cacattctgg gcatttcttt tgggtaatcg
    40501 agaaatcttg atattcttgt ttgcttgatt tgatctcatt caccaagcgt tttgtttctt
    40561 tttcgattcc attcaagaag gttcctgctt gtttctttcc tgcagcaata gcttcaaggt
    40621 ctttttccca gctttctgtc aaatctggtg ttactaaaga gggatttacc aatactaata
    40681 attgcttacc tttaggagat acttgcagtc cttgaggggt acgttccatc agttcagatt
    40741 tgattagttt ctcgataatc tctgcacgcg tagctggggt tcccagactg tatttttcca
    40801 tttgaccaag caagcttgct tctgacaatg gtttaggcgg tgcagatagt tcttttttga
    40861 tggcgaaatc gggattgatt ctcattcctt tttcccattt gactgtttcc gtctcagttt
    40921 cagtttcgcc tttccatcct gcttggacga cacgattttg ccgaaaaatg aatcgttctt
    40981 taccaaattc aaccgtaact tttgtttggc tgactttatg gggttgagca aacagtccta
    41041 aaaaacgaga gacaatcatt tgatagattt ttcgctcttc attacttaat ttttcataac
    41101 gtgctctgtt ttcagttggc aaaagtgcat ggtgatcggt cactttactg tcttggaata
    41161 cttttgtctg ttggacgatg gctccgtttt tcaaatactg cttgacctca ggagcaaagt
    41221 cgctgactgc ttgcaatcgt tctttcatcg tacttttcat gtcatttgtc agatatttgc
    41281 tgtctgtacg cggataagta acgatcttat gggtctcata caaactttgt acaagactta
    41341 atgttttctt tgcagaatag ccatagcgtt gattggcttc tctttggatc tctgttaaat
    41401 cataaggcaa tggcgcagat tccgttttta atttttcttg gacttctgtg acagttcctt
    41461 tgcttgtact gatcaattga acaagttttt gtgcttcttc ttgtgttttt aaagcaaatt
    41521 gatttttttg aagcattttg gcttgttctt ttccagcttg caacgtgatc gtgaaatacg
    41581 tttgtggacg gaactgctcg attttctttt cttggtctct cactaatgct agtgtcggtg
    41641 tctgcacacg gcctgctgat aaattatccg cgtacttcac agttaaggct ctggtcacat
    41701 tcaatcctac cagccagtcc gcttttgccc gggcaagagc agattcatac aatgcatcat
    41761 attctttcgc aggtcttagt tgcttgaatc cagttttgat tgctttatct gtttgagaag
    41821 agatccataa ccgcttcact ggtttattga aatgcacata ctctaagatc cagcgagcaa
    41881 caagttcccc ttctcttccg gcatctgtcg caatgactgc ttctgagaca tcttttcgct
    41941 gagccagctg cttgattgct tttaattgat gtcctgtttt aggaagtggt ttgattccta
    42001 gttttttagg gatcattggc aacgtgtcca tttgccagct ctgccattcc ttattcagat
    42061 cttccggcat tttcaatcct aataaatgac caagggccca agtaacgatc accttgggtc
    42121 cttcataata atttttgtgc ttttgattag caccaagcac cttgcttagg tctttcgcaa
    42181 cgcttggttt ttccgctaga attagttgtt tcataaaatt cctttctgct ttctactgtg
    42241 atgggctgat gatttttcaa caacgcaagt cccttgtatt gctgtaaatc atcgtcacac
    42301 tcttcacacc agcgctcatc tccgtcattc caaaaggcgc tgagaaagtt actttttgca
    42361 ttgatatatt cataattttg atggatttcg ttccattgtt tttcgtacgc ttgtcgtgct
    42421 tcttcgaaag actcgaatgt ttcttcttct tcaatatctt cttcccagtc ttcaaaaaac
    42481 caccaaggct cattgtcccc gaacatggta attacttgat acatactcgt gctccttctt
    42541 tcagcttttc tagactcatc ttagtcactt ttcaagaaag ttgcaagaat tttctcttat
    42601 aaaagggtca tttaatgaac atcttaaaat tgttacaaat gaatctgatt gtttaaggca
    42661 aagtagcgat aaagttattg atcatccaaa aatagctgtc ttctggatcg attgttggtt
    42721 cttttgtaaa gtatcctaaa aaacgtaagg aaagataacc gtggatcata ctacgaagtt
    42781 ccctgcttcg atgaactttt tcttctttcg ttaattgaaa agcctcaaat atctgcaaaa
    42841 tgatctgatt cgtttcatgg attccatcta ataaaacagc ttcattggta tgcggtacac
    42901 tgatcagtaa ctcatacact gcttgatttg aaaaagcaaa ggatcgataa gtttctgcat
    42961 agatctgcag tgcttcctga ccactttttc ccacaagagc ccgtcgcagc tggtcattaa
    43021 gttcctgcag aagtttcgct gttagcgctg ttttgacatc acgaatattt ttgaaatggt
    43081 tatacagcga gggagccttg atatccaagc gtttggctaa atgctgaaag gttatttgct
    43141 gaacatccat ctcttctgct agttctcgaa agcagtcgat gattttctct tgagacagat
    43201 ttcttttttt catttccatg tttcctctca taaaataaat ccttcactct ttcagtttac
    43261 cacgaaaaag aaagagaaaa aagagacttt cattgataaa ctacatattg tagtttataa
    43321 taaagagctg tagatgaaaa ttttcagaaa gaaggagtct aattggctgc aagaataaag
    43381 gaaaaattat ttaggaaaag agaaacttgg gaacaaaact tgtttgtctt atggtttgga
    43441 acatttatgg ctggtatcgg atttagttta gtcatgcctt ttatgtcact ttacatagat
    43501 acgttaggta attatagtac gtcacaattg aacttttgga gcggattgac tttttcatct
    43561 actttcttgg tgactacgct tatctctcct tggtggggaa aattagccga ccaaaaagga
    43621 agaaaattga tgctgttgcg tgcttcatta ggaatggcaa tcgtcatttc tttgatgggt
    43681 tgcgtgacta gtgtgtatca attagtcgca ttgcgtctgc ttcaagggat tttttctggt
    43741 tatatcagta atgccactgc ccttgtcgca actgggacgc caaaagaaaa aagtggtcaa
    43801 gttcttggaa ccttagcaac tggttctgtt acaggaacat tacttggacc gcttcttggc
    43861 gggatctctg cttcagcgtt tggctatcgc cctacctttt tcattacagg gatcatctta
    43921 tttttcgtct tcttgttgag tctgtttttc gtccatgaaa aatttgtccc agtgaaaaaa
    43981 gaaaacatgg tttctgccaa acagattttc aaagacttga aatatccaca cgtcgtcatc
    44041 ggtatgttcg tcacaacgat gatcatccaa gcatccaaca attccattag tccaatcatc
    44101 agtctgtaca ttcgccaatt gctgcatgga catgggaatg ttacgttgat cagcggaatc
    44161 attgcttcga tacctgggat tgccactttg atagccgctc caagatttgg tcgcttaggt
    44221 gacaagattg ggagtgaaag aatacttgcg attggtttgg gttttgcgat tctggtttat
    44281 atcccaatgg cattcgtcac gaatgtttgg caattagctg ccttacgttt tttgattggt
    44341 atctctgatg cttgtctgct ccctgctgtc caagcattga tcacaaaata ttcgcctcat
    44401 caagctgctg gacggatctt cagttataac caatctttcc aagcaactgg taacgtagta
    44461 gggcctatga tcggctccag tgtttccagt gcatttggtt atcgtggcgt ctttctttcg
    44521 acttctcttc ttgttttggt caacttcttg cttgtacgac acaatacaca agagatccat
    44581 cagcaagaaa ctgcagaaga aaaagcttca ggagtctcca tagcttcagg gcactctcct
    44641 tctcatagac aataagcgta aatgaaaaac actgcttgaa cagttgtcac agactgtcct
    44701 tgcagtgttt ttgtttaggc ctgttttttc aagaactcag ataagaggaa taatcccttt
    44761 tccaaggttt cttttttaca acagtaacct agtcgggcat gcttcggtaa atcaaaggca
    44821 cttcctggta ctaatagtac tcctgtttct tctaataaat tgatacaaaa tgtctggtca
    44881 tcttcttcga tggctaattt aatgaaagaa gtagagacgt aattgggagc gacaagttca
    44941 actttcggtt cattggctac ccattgtttt aaaatcacaa ggttctctgt aatgatcttt
    45001 ttgtttcgcg ccaatatttt ttctttattc ttcaatgcat gtacagcaag atcatctgac
    45061 aggactccgc cgcaaatcat cgtgtagtct cgatactttc gaaaaacttc tgccaactca
    45121 ggtccagtag ctgtccagcc aatacgaata cctggaatgg agtaggtttt ggatagggaa
    45181 ttggttgcta tacctttttc atagacatct acgatcgaca agaattcacc tttatccgtt
    45241 aaaggcgcat atacttcatc tatcaataca tatgcatcta ctgtacgagc aatttctgcg
    45301 attttttcta aagttttccg atctagcaat gtgcctgtag gattgtttgc actattcaaa
    45361 cagatgattt ttgtttcagg tttgactaag gcttctagct gttctaaatc aaactgccaa
    45421 tcttcttctt ccttcaaatg aacaaagtca acagcggcac ctaaagattt ggggatgtca
    45481 tataattgct gataagtagg aagcatcgaa acaacatggt ctcctgcttc cactaacgcg
    45541 tatagagcta aaagattagc accagtcgct ccattcgttt gtaagatgtt ttccggatcg
    45601 acggtttgat aaagagaagc gacttcttgt ttgaaaacat ctgatccttc gatccagcca
    45661 tagtccaatg gttcgttttt gcgtttttcg aaaaatttac ttacgctagt cccatctaag
    45721 ccaacgagtt cgtctaatgt taatgcttcg atcgagcttt gtgcaatatc atataccgct
    45781 ttcttttccc atttatttag ccattcttct acaccgaaat ttgcgatttt catgtttctt
    45841 tcccccttat atcttgatcc agcctacagc aagcaacccg attgaaagca gtgcggccac
    45901 acaaatcagc ccaatcatta ctttttcttt tcccgtaatt tgatgtttgg tttcacgaca
    45961 agcgatccag tagaagcaaa aaccaggaag ataggcaata ctacataaaa ggacataaga
    46021 aaatcctgct aaaatgatgc ccatcaattc aaaaactaca gcaatcccac cgatcactac
    46081 ttgttttttg attccttctt ttaaagaaaa ctggacttga tagatcccga caagtagata
    46141 acaaatcaaa attgccgctg tacataaaga ataggcaaat tgataggcat aatcagtaac
    46201 taagaaagtc aataagaata gattagtcaa gattgcagta acaactaacg aataagttgg
    46261 ggcatttttt ttattcgttt ttccccaacc tttcggcaac agaccgtctc gagccatcaa
    46321 catcgttgtt tctgctggga gcatcgtcca agacaaccaa gaaccaatga tcgaaataat
    46381 caagccgata ttgatcagcg ctgctcccca atttcccacg attcctttta aaatatttgc
    46441 catagcaggt tgtgaaatag ttgctaactc ttcttgagat aagacgccgt atggcaagat
    46501 cgaagcaagg atatagataa acaataaaca aagcagacca atgatcgatg cttgctgggc
    46561 ttctgaacgt ttcttcgcgc ggttggctaa aacacttgcg ccttcgatcc ctacaaagac
    46621 ccacatcata accatcaaac aacctttaat ctgcaaccag atcgttcctt gctgccctgt
    46681 tccattcact agattatcag atacgttacc ccaaaaatca gcagtgaata tattcacttt
    46741 aaatgcgata aatacaaata ttaaaaatag gaataatggc actaatttac agatcgtcac
    46801 gattgtattg atgacgctcg ctccttcaac tccattgttt actaataaag tcagacccca
    46861 aataaaaata cttgcaaata aaatagaagg aacattttgt ccacctttga atatcgggaa
    46921 aaattctccc atggcgctca tcagcatcgt tgcaaaagca acatttccca gccacgcaga
    46981 caaccagtaa ccccaaccac tgatgaagcc acctaattta ccaaatgaag cttccgcgta
    47041 accgaaaata ccaccatcta gatctggacg cttttctcct aaatgattca gcgataatac
    47101 caaagctaaa ataccaatcc caacgattat ccatgaaagg atcgctggcc ctggagctgc
    47161 agaactagcc agatcacttg tgatcccaaa cacaccgctt ccgattgacg aactgacaac
    47221 cagagcaacc agtccccatt tgctaatacc cgtcgatttc ttttccattt ccatcaccct
    47281 cacttataaa attgaacaaa tcacttgcaa aagattccct tctattttgc aactgctctt
    47341 gcttaacaaa aagcaacttt attcatatta ttggatatta tgaacttttt acacaagtat
    47401 ttcaagtata aaatgaataa aaattaattt tattgcaaaa aaacagctat gctatccaaa
    47461 agaaaaggtg tttcttttgt tagccatagc tgttttgatt catttaatat cgcttaaacg
    47521 tattgtaaaa cttttgtttc ttgatatgcg tggtcgatca aaccaccacc cagacattcc
    47581 attccatcat aaaatacaac tgcttgtcca ggagtgattg cacgtacagg ttcgtcgaat
    47641 acgacttctg cacgattatc atccaataag cgaactgtca caggaacgtc ttgctgacga
    47701 taacggaatt tcgctgtaca cttgaattct tttggcattg gttcattcgt cgtaaagtgg
    47761 atttcacttg catccaagct tgtggcatac aatgcaggat gatggaaccc ttgccccaca
    47821 tataatgtgt ttgtcgctag atcttttcct acaacaaacc agggttcttg cgaatctcct
    47881 ccgccaccga tccctagtcc ttgacgttga ccaatcgtat aatacatcaa gccagcatgt
    47941 tgtcctttga cttcaccatc aagagtgacc atatttcctt tttttgctgg cagatagttg
    48001 ctcaaaaatt gtttgaagtt cttttcacca ataaagcaga ttcccgttga atcttttttc
    48061 ttagcagtcg caaggcctgc gcgttcagca attgcccgta cttctgattt ttccatgccg
    48121 cctagaggga acatcgtttt tgaaagctgt tcttgtgata attggctaag gaagtaagtt
    48181 tgatctttat cattgtctac tccacgaagc atgtggacca cgccgttttc atcgcgagtg
    48241 acttgcgcat aatgtccagt agctacataa tcggcgccaa gatccattgc ataatccaag
    48301 aatgctttga atttgatttc tttgttgcac atcacgtctg ggttaggtgt acgtcctgcg
    48361 cggtattcgg ctaggaaata ttcaaataca cgatcccaat attctttttc aaaattcaca
    48421 gagtaataag gaatcccgat ttgagcagct actttcgcta catccttata atcttccgtg
    48481 gccgtgcata caccgttttc gtccgtgtca tcccaatttt tcatgaatat tccgatcacg
    48541 tcgtatcctt gttcctttaa caaaagtgct gttacagatg agtcgacacc gccgctcatc
    48601 cctacgacaa cgcgtgtttt gctgttgtct gtcatgatat caccatcatt tcaattagat
    48661 tctgaaacat ctacgagttg aaggtcgcaa cttttcagta ttttacatta tacgtcatat
    48721 ttctgaaaat ttcatccctt aattatcaag tcgtaaaaat actgttctac tggacctatt
    48781 cagtacacaa acatgatagt caaccaaaaa atcactcatc ttttgagcat tcgatataaa
    48841 aaattggcat actcataaaa agggccagcc tttcggcaat aaggaataat tttataaatt
    48901 gtgaaaaact atttttcaaa attgcttctt atcgcttaaa gctgcacgtc tcatttacaa
    48961 ccttttcttt ttagatgtta tgagttgctc agataagtga ttttgtttac tctaagaaaa
    49021 aagggaggca aaagatacat gaattacttt atcagtgata tgcacttttt ccatgaacgc
    49081 ttattaggaa aaaacgactt tgctccgcgg ctctttgcaa atgtggatga aatgaaccaa
    49141 cagttgatca ctagctggaa tgcagtaata aaagaaaccg accatgttta tcatctaggc
    49201 gaccttgcta tgcatccgca atatgaaaaa gcaaatgcag aaattttgga actggtcact
    49261 caactgcaag gaacaatcca cttgatcaaa gggaaccatg acagtcgcgc ttttttccgt
    49321 tatttagaaa aaaatgatcc taggcttact gacggaagtc ccaaatttta tttttatgat
    49381 gtaggtgcca tcgttaaatt cgaccatcaa caatattact tgactcatta tccgatgttg
    49441 ctcgggccta acgacactat ccgcaatctt catggacata tccatcatta cagcgtacct
    49501 atcgagaacg atgtcaatgt aggcgtagac gcaccggaaa gagaactctt aaaggaaaaa
    49561 ctaccatttg gcactccgtt atctgagcaa caaatcaaag aaatctatga agcaaagagg
    49621 aagtacgata ggtcgtgaaa aaactgccct atatcaagta ttaaacaaat aaagataatt
    49681 actcatattt ttcatttgag taggcttatt gccacaggaa cattaggatc agaaatggag
    49741 ttgtaacaag acttctgtca caactccatt ccatactatt cgtctttttg ctcatggtgt
    49801 tcttttacca gttttttctg taattcttta tcataatttc gggcagctga cgttaaaaat
    49861 aaaacgattc cgcataataa aatcccgatt ccggaaaaca agaacatttt ttctacccct
    49921 aaacgatcag ctaatggtcc tgcaaataat aatccaattg gacctgccag gttcatcaaa
    49981 gagttgaagt tccctaagac acgacctaaa acattcgatt cataactctg ctggatcatt
    50041 gccatcagca gtgtattgaa atatggcgtt gcacaacctg ctcctgcatt caagataaga
    50101 aagtacaaga acccttgact atcgtttgga accaatccgc ttgcaccgat cgtcaggccc
    50161 ataatgatgt aggctataat aataggtttc attcggtctt tccatgttcc tataaaactg
    50221 atcagtgcac cgcctaagag cattccgact gcgtagacta cttccactgc tcccgcctgt
    50281 ccaacggaac caccaaagta atctagcgtc atcaatggat acagactgat tgctggcatg
    50341 aacaacagca tcacaaatgc accattgatc gttatgtacc ataacccttt attatccatc
    50401 aattgctgca gcccaaattt tgcatcttta aaaatagtaa gcggaacttc caatatttca
    50461 ggagaaactt ttgggatctt cacaaaaatc aataagccga caccaaaaac tgcccccaat
    50521 acatctaata aaataaggta attgacagga acgactgaaa acaaagccgc acctagagct
    50581 ggcgcaatga tgaaattcgc tgattggacc attcctaact gtccattcgt ccttgttatg
    50641 tgagaagatg gaacgattgt cggcataatc gactggatcg ttggcatttg gaatgtttgt
    50701 gcgaccgaac gcataaacaa agacacaaaa actagccaaa tcgggaagga ttctgaaatc
    50761 gttccgacta ctgctaaaat aagcgcgaaa atcgctacaa taatatccgt gacaatcaaa
    50821 agagcttttt tatcccagcg atccactagc ggtcctacaa atgggcttag taaaaccatg
    50881 ggaatcattc ctagtaaagt agcaaaactc aaaattgtcg ccgaaccggt ttctcttgtc
    50941 aaataccaga tgatcgcata ttggacgacc atactagtga ttcctgacaa aaactgtccc
    51001 gataaaaaga acaaataatt tctcttccaa ttcgttaaaa cgaccgactg ttcattttcc
    51061 ataaacatcc ctcttttctt tttcattttg tatagcgttt gcacaacaat cattatacgg
    51121 gattcttttt tcaaaaaata cctttttccc taaataacaa tcgtttcaat ttaaatttct
    51181 ttacttagaa acaataaatc agcaaaatat taaaaataac aacatgtgtt aaatttaaaa
    51241 ataaaaaaga caactaattt gcgtcgtcct tacaaagtta gtcatctaac aataggccgt
    51301 ttcaaaacta gttgtctttt tatatccaca gatcattcga ctttttcgaa taacccacgg
    51361 ctaatcatcc catccatcag aaaataaaat ttatcttggc tcattttctg ttttggatct
    51421 ttgaaaatat tgactgcttt caatccattt gctgtagtga tcgacatttt caatgtttga
    51481 atatcttttg atacagtgat ttttaatttg aatccttctt tgctttgcgg cgtggcatct
    51541 aatggtcgga tcaattgata attgcctccg tttgtttctg taaatccatt atcgcgcaat
    51601 gtatatcttt ttgcatcagg atgcaaacga tagctaattg gtgcgcccag taccgttgct
    51661 gttgtttcaa atgccatgat tttcacctcg ataaaattag tttctcttta ttataaaagt
    51721 ttatcctgat ttttactagg tttttcagtt aagttcccta aaaaatgaag gcgttctctc
    51781 agacatctgt tcatataaga atgacttcac tagttatttt ttgacagcac gaactaattg
    51841 atcaaggaaa tactggatgt cgtcttcggt atttcccaaa ccaaagctga tacggatgga
    51901 ttcttttagt atcggtgtat cttctccata cattgcttct aatacgtgtg aaggctcgat
    51961 atttcctgct gtacaagctg aaccagtaga aatagcgacc ccattaagat caagcttcat
    52021 cagtagcaaa tcatttgctt cgccaattaa gcgaagattc aacacatgag ggagtttatt
    52081 cgttaaatca ccatttactt gataagggat ttcgtttgct tctaatgtat caagaatcaa
    52141 ttgctggaat cctttatatt tgttgttacg tgcttcacgt tcttcaggtg acagcagttc
    52201 aactgctttt gccatacccc agatccctgc taaattttct gtacctgcac gccgcttttc
    52261 ttcctgctcc ccgcctaaaa gcaatgcagg gatatttact ccgtctcgtt tatataagaa
    52321 accgacgcct tttggcccat tgattttatg agcagagata cttaataaat cgattcctaa
    52381 ttcttctgga tggatcgttt ggtttccgta cgcttgtacc gcatctgtat ggaaataagc
    52441 tggatgatcc gctaaaagtt caccgatttc tttgattggc atcaaatttc cgatttcatt
    52501 gttcccgtac atcacagaga ccaagatcgt atcttctcgt aacgcttttt tgacctcttc
    52561 gatcgacaga ttcccttttt catctacagg cagatatgta acgtcaaacc cctttgtctc
    52621 taaatatttc atggtattaa gaacagcagg atgttcgata gctgtagtga ttagatgtct
    52681 gccttctttt tgacgcgaga acgctgtttc taagatagct gtattatccc cttctgttcc
    52741 cccactgtta aagatgatct cgtgaggttt tgcttgcaga ctgtctgcaa tgatttgtct
    52801 tgctgtttcc aatttttcat gcgcgtggcg accaaatcca tgtatgcttg aaggatttcc
    52861 aaacgtgtct tgcatgattg ccgtcatcgc ttcaattact tgtggatgca tgggggtagt
    52921 tgccgcatga tccagataaa tagttttcac ttcattcatc ccttccaaat tattgctagg
    52981 cacattctag cataaaatag cgaacaaacc aaagaagagc tcatttttgt ctagaggacg
    53041 tttcatgggc atagaaaaaa ggagctgtga caaaagtctt gtcgcagctc cttgaagctg
    53101 ctcacttctg tcacaacttc tttccctctt ttttttgttt attcgaattc tttttcttcc
    53161 agacggaata acggactcat cggtgtattt tcatggatgc gtttgaccgc ttcacccatt
    53221 aattctgaac atgtaacgat tgttaagttt tctggatgac gattttcttc tgtgaaaaca
    53281 gaatcagtga tacaaatatc tttgattggt gcattgtcta aaatactttt cgcatgatcg
    53341 gacaataatc catgtgacgc acaggcatat acttcttttg cgccgttttc ttttaataca
    53401 tttgctgctc gtgcaagcgt tgctcctgtg ttcaagatat catctaccat gatacatgtc
    53461 tttccttgaa catcaccgat cacataacca ccctcagttg aagcatcatc tgcatgatct
    53521 acgatggcta atgtgctgtt caaatattct gaaagactac gtgcacgctg aacaccgctg
    53581 ttttttggcg aaacaactac gatgtcatct cccgtcagac ctaattgacg gtaataatga
    53641 gcaaacaacg gcatcgtaaa taaattatct acaggaatat caaagaatcc ttgtacttga
    53701 accgtgtgca gatctaaagt cagtactcga gttgcccctg cttcttcgat caagttagcg
    53761 atcaatttcg cagtgatcgg ttcatgaggt ttcgccgtgc ggtcttgcct agcataccca
    53821 tagtaaggca atacgacatt gatcgttttc gcacttgctc gcttcattgc atcgatcatg
    53881 atcagtagtt ccatatagta atcattgaca ggtgcattcg ttgattgaac gatatacaca
    53941 tgatcccctc gaatactttc ttcgatattg atggaaatct cgccatcact aaattgcttc
    54001 acgacgcttt tacctaattc tgttccgaac actttcgcga ttttttcagc taatggacgg
    54061 ttgccattca aactaaaaat cttcaacgtg tcatcctgat acttattact catgtagtcc
    54121 tccccaaaac ttctctattc ttcgatagtt taacatattt ttccaagatt ttctgttaat
    54181 ttcagcaaat tcttaaggaa gtgaaactgt tccgttactc caactggcag gaatgacttt
    54241 atgataatcg ccccagttgt cgtgtacttt gtaataacgt tctcctgttt cagtttcaaa
    54301 ataagcgtac gctgtcaccc aatgattgcc atatgtactg ccaagtactt tcaaaatacc
    54361 aatcatcaca ggtttgcctt cacgtatacg ttttgtagct cgctgccatg aacctagaag
    54421 agttgctcgt gcacgctcat gattgccatt cttctttaaa aaactagaaa tccctgtact
    54481 aacctgaaaa ggaatcgtag gaagtcctag tggttggatc tggctacgca atgccttcgt
    54541 caaaaccaac gaatcagccg tatttttctt gcgaatctca tttggaagca tccacccctt
    54601 acgatagtct tgataatagg caaggagaac tgctgccgcg taagtaccac aaagatagcc
    54661 tgagggggta gcccatttct tatacctcaa aaaacgttct tctggcaatc ccacccagca
    54721 atttgcgagt tccttttctg accttttttc ttcgatcaaa acgggaatat cttcttttct
    54781 gttgatccat ctagataact cgattggctc ttcattgatc agcttccatt ttttacaaga
    54841 aagaccctct tgataagtat attttgctgg agaatattct accggatgtc ctgattgatc
    54901 gataatcaca taactttgat cttcaaaaac cagagctaaa aaaagcaatt tactttttct
    54961 tgactgcagc tgctgagtgc tggctaattt tttaattttt ttatctactt tgaaatataa
    55021 aggtttatcc actacttgat tcagttggga tcgcctcctt tttttcatta taagtgaagg
    55081 attgaaaaaa acaaatcagt ttgattgttc gaattttcaa ttttttatgt tctatttgtt
    55141 aattccttca ttttgtaaga taatgggcac acgaaacaaa aaaggagcga tcgatgaatg
    55201 aacaaagaac gattgaaaca agatttctat gaagcagtca atgaagaatg gcaaaaaaca
    55261 gcaaaaatcc ctgcagataa accagctaca ggcggtttcc aagatttagt tgacgggatc
    55321 gaccaacttt tgatgggaga aacagacgat ctgcttgccc atccagaaaa agtgtcttca
    55381 gaaaaaatga aacatttcct tgcatattat caactggcaa acgattatga ccgaagaaat
    55441 gagttgcatg ctcaaccgct tcaacctata atcgaaaaag ttgaatcatt gcagtctttc
    55501 gacgaattga ataagcaatt tccagaatgg ctgttggata cattaccatt gccatttaat
    55561 ctggatgtag atgcagatat gaagaatgcg caaacgaacg ccttctttgc ttatccgcct
    55621 tctttattcc ttccagataa gacgtattat gcatcagacc atcccaacgg tgcacaacta
    55681 ataggcgttt tctttgatat ggctgttcag ttattagagc ttgccggcaa agaaaaaagt
    55741 caggcagaag caatcacgga acaagcagtg caatttgata aattgattgc tcctcatgta
    55801 aaaagtgcag aagaaaacgc agattacagc aaaatgtata atccgcaatc ttttgataca
    55861 tttattaaat acacagataa actggacttg aaaaaacttg tcacgggttt gattgggaca
    55921 actccagata aagtgatcgt aactgatcca gtgtacttcg atcatttgaa tgacatcctg
    55981 acagaagctc attttccgtt attcaaaagc tggatgatca ttaaaacagt acgttctctt
    56041 agtggttatc tatcagaaga attccgccaa gtcagcggaa tctattctcg cgtattgtct
    56101 ggaacagacg aagcaatgcc tcagaaaaaa gcagcgtatt accttgcctc tggacaattc
    56161 gatcaagtag ttggcgatta ctacggaaaa aaatattttg gcgaagctgc taaaaaagac
    56221 gtagaacaaa tggtgaaaaa aatgatcgcc gtttataaaa aacgtctaga agaaaatgac
    56281 tggcttgggg atgcaacaaa agaaaaagcc gtcataaaat taaataactt aggtatccaa
    56341 gtaggctacc ccgataagat cccagcatta ttcgaccagt tcaaaacagt acctgcagat
    56401 cagggtggta ctttgctttc aaatactctt gttttcagca aactcacatt aaaagatcgt
    56461 ttcagcaaat ggaacaaacc agtggatcgt tcagaatggg aaatgagtgc tgacacggtc
    56521 aatgcttatt atcatccatt tcggaatgtg atcgtctttc cggcagcgat ccttcaagca
    56581 ccattttaca gtctagaaca atcaagctct gctaattatg gtggaatcgg agctgttatc
    56641 gctcatgaga tttcccatgc atttgataat aacggcgctt tatttgacga atatggcaac
    56701 ttgaacaact ggtggacaga agaagatctt acacaatttc aaaacaaagc acaagcaatg
    56761 atcgaagaat tcgatggtct gccttttgct gacggaaaag taaacggaaa attaaccgtt
    56821 tcggaaaata ttgctgatgc aggcggtcta agctgtgcat tggaagcagc aaaaacggaa
    56881 tcagatcttt ctatcgagga attctttgtc aattgggcaa cgatttggcg tacaaaagca
    56941 aaaaaagaat accaacaact tttactgcaa atcgatgtcc atgcccctgc taaactccga
    57001 gcaaacgtgc aaccaaaaaa ccttgacgaa ttctatgaaa catttggtat cacggaacaa
    57061 gacgctatgt atctagcgcc agaaaaaaga gtgcgaatct ggtaagaggt tgtgccagaa
    57121 gtggtcagct tcaagaaata agaaggaatt tgggaaaatc gcttctcaaa tttttgtgac
    57181 atttcggctt atttttcgaa gaagttactt ctggaatacc atgtattaga ggttgtgaaa
    57241 aagctgctta gtttcaagta gtaagaaaaa ttttgtgaaa atggctttcc acatttttgc
    57301 ataaatttga cttactaccg agaaactggc ttttgaacac cgtttatcga gaggttgtga
    57361 aatcagcgtc ctgacctgag tattagagaa aaaaacggaa aaatagttcc tcatattttg
    57421 ccgtttgttg agctaatacc gagggtcagc tgattgaaca ccgtttagtg gagagattgt
    57481 gaaaaagctg tcctgcatca agtaataaga acagccaatc aaaagcagca aggagaacct
    57541 catctataaa aagatagaag gaagtgagac atctattggt ctcatttcct tctattttta
    57601 ctttcctcag tcaattgttt tacatcataa aaggagaaat tccacttatt tttttcgctg
    57661 tttcatgaca taaacaacta caccgattaa caaaattcct ccagctaatg ctagcaacgg
    57721 gttattaaat tctcccgttt ttgggagtcc ttgagtgcca tttttttcac tttcttttgc
    57781 tgtcctatcc ggatgagaag gttttccatt tggttttcca tttggtgatt catttcctgg
    57841 attttctctt tctggtttgt catttcccgg gggcacatcg gcagtttctc gcacagtgat
    57901 cgctttttgc tgggtaacag attctgtatg agcagaccat ttttccccat caaatatttc
    57961 cgaacgataa gtgacttcta gcgtatattc tccttttgaa ggtgctgaaa aaatagcggt
    58021 atgattagaa aattgagcac ttgactgtgt ttcaacgatt tggatctttt ctgggaaata
    58081 acgcatatct tcaaagacag gcgttccttg atttggtgtg aaagttgaca caaaaactgt
    58141 aaaaggcttc agttcaatga tatttccttc tgcattctcc gccacaccat ttgcttcttg
    58201 tataataatc ggattatttt gttcatttgg ttttgtctga tttggttctt gtacgattgc
    58261 atactctgga tattccgaag acggtgaggt ttctccgtca aaaatagcac ctacagataa
    58321 tggaagacca tccactcttt ccttttctgt ttgacgattc cccggtttaa tctgcatcag
    58381 tccatgaaca tggatacttc cattttcata gcgcgtaaca ggctgctgac cttctttcga
    58441 acatgtattt agagactcga tccaatcttt tgatatggta tgtccaagag tattttctga
    58501 ttgtttctct cgttcattcc aatagtagtt caacgagtgg tagatttgat cttcgttttg
    58561 attcaatggg cggatctgct ctctcatctc aagattctct gttcggaaag ataagatacc
    58621 agaagcatgg tagtctgtgt ttcctgctga agaggtataa agagccacat tatatgaacc
    58681 gttattaaaa cttgtactgc tattgacgat aatatctgtt ccgctattgg aatcgattcc
    58741 tttcgcaaga ttttcgaaag acaaactatt gatcaattca tgagggcccg ttaaagaaga
    58801 accgcccatt ttgaatccat taccgttacc ttctccctca attcctggta cccagccgtt
    58861 tcgataagca actgagtttt tgatgattac ttttccgatc gaacctgttt catttttagc
    58921 aaaaaggtcc caaccgtcat ctgcattatg atacgcgata caaccgtcaa atacatttcc
    58981 ttctcctatt gtcagtttgg ccgcaaatcc atcggcatct tcaaatcctg gatcggcatt
    59041 ttcaaaagat gtacagttct tgactaagtt atgcgcgggc catgcttcga aaggatcatt
    59101 tgagctaccg ctgatttgga ttcctgtatt tccattccga taagcatgaa tctcttcgat
    59161 caaattatga tttccagaaa tctgtaatcc tttttgcata gctgcactat ttgtaatgtc
    59221 aaaccctcgt aaaccccagt agtgactgaa cagtgtcatc ccatttccag ttcctttaaa
    59281 atcaagtaca ggacgcttgt tttctggatt tttctctgca atcaactgaa tcgggttttc
    59341 tcttgtcccg ttaacgcctt ttggaatcgt caatcctctt tcgaaggaat aagttccagg
    59401 cgccatgacg attgtctgcc cagcatatgc gtatctcaat gcttgtgtca gagataatgg
    59461 attttgtctg cttccgtttc ctgcatctga cccttctggt gttacataaa ctgtcgaata
    59521 tttaggttta cggtagtcca ctgtatgaag aatctctttt gtttcataac tagtcatctc
    59581 tacgtattct tctggcggat agtttttatc tggcgtaaaa ctgaaagtaa attcgttcgt
    59641 tccttccatc agttttgtct gataatcaaa ttcccgattc gcagtgatag ccaagttttt
    59701 tgcgttctta agcacctctc ctgttgcttt atttttaatt gtcaataatc cgtcagcatt
    59761 tgctctaaag gacatagtat gatcttggtt tcctgaatga ttactagagg tcacagtgta
    59821 aatcggatca atcaagcgag taggtctttc ttgcccagga ggatctgtcg caggatctga
    59881 gtaagcaact tcgattcctt ctaccttgat cgtcatattc cttgctgcag caaatcctgc
    59941 ataaataacc gattcatctg aagcgtataa tttttcccag tcatacatga tgtcagaagc
    60001 catttcttct cctgtttcag gatcgtaata atatgcctct aaaccagtat tcgttttttt
    60061 catctctacg tccagtttag taatcgagtt cgtggccgga aacagattcc catgaccgtt
    60121 tccgaatata ttataagaac ctgtttcttt ttccaagaac cctgctgaag tctctaaagg
    60181 tgttgtttcc actgagacac tcccaggagc tggcgcagct tgcggatctt cagatgatat
    60241 tcctgtgatt gcccgtgtcc ccaatccaag tcgcatcgta tatttgctgc cgctcgtgtt
    60301 gaccacttct tgtctatccg agtcccagac atattcgatc cgactgccta gtatggcaaa
    60361 tgaatttgaa taaaatgcct gcccaaacaa atgatttttt ggtactgcgt ctcttaccat
    60421 taaagcaaat ccttcttggg cattcgtata ggtccaagat tctatttcga tcgtcgcccg
    60481 catcctaaag ttgatgtctt tggggattgc ttgataataa taggtcattc catctgatcc
    60541 gcttgactgg aattttccgc cgttattcgt ggaagagagc cgcacgccgt cttctaatgg
    60601 ttcaatggca tacctactag cacttgtgct ttctccgaaa acaacccctt tccactcttc
    60661 atttgcttct tcagattgct tttttgtctt ttcattttct gattctttca tcataggatc
    60721 atttccttct tccgtatcca tatcttcact agttcttggt atttcctcca ttgttgacat
    60781 cccttcaatg ttcacgtgtg cattttcttc gttcacagac tgttgttcgt tagcagcaac
    60841 ttcaacgaca gaaattaaaa ccggggtact gagcataagg agattcatca ttagcgcact
    60901 gtattttata cgttttttta acaagtgtat tcctcccgta acaaattaga aagcgtttac
    60961 ttaaagtatt acctaattta tttttactgt caataaaaaa caaaattata ataagtgtat
    61021 tttataaaaa gaaaaaatat aacttaagtt gttgataata aagagattta ttaacgtaaa
    61081 aagaataagt ttttttaaaa taacgtataa aaaagagcat aaaacaaagt cgtttgtttt
    61141 atgctcttct tttattattt tgtgcttttt attttatcaa atcactttgt ttttttataa
    61201 aatttctgcg cctagtgtgg actcaaaatg tctcaatgcc cattcatgtc caacgggatc
    61261 gaaacttgcc acggcatctt tgaaaatatg gagtttgtat cctagattat acgcatcgac
    61321 tgctgtatgc aacacacaga tatctgtaca tacgccggtc aaataaatat ctgtgatctg
    61381 gcgttctcgc aaacggatgt ctagatctgt tccactaaaa gcggaataat gccgtttgtc
    61441 tatccaataa acattttcct cttgctcata gtcttggtat aaacttttta aagagccgta
    61501 caattcccgt ccacccgttc ctatcacatt atgcggagga aataagcggt tctccggatg
    61561 aaaggaatct tttggatcat gcgcatcaat ggcaaaaacg acaaaatcct tttgatcaat
    61621 aaacttcttt gtatgagcag tcagcttttt ttcaattact tgtcctgctg tcccagtagt
    61681 cagctttcca tcctctgcaa caaaatcata tgtgtagtca atcgagatca atgcgttcat
    61741 cttagtcctc cttatacgag atgtttgaca aattcgatca ccatttctgc tgatcgtttg
    61801 cctgcgtctt cgataaattc gtcaaagctt tgtgtcgccg agtgatctgc tgtgtcgctc
    61861 attgcacgta cgatcaaaaa tgggatattg aactgacgag ctgtttgtcc aacagcggct
    61921 ccttccattt cgcaagctaa tgcttcaggg aaattcgtta agatttcttt gattttatct
    61981 ggcgagtcga caaatgtatc acctgtaacg atcaatcctt cttttgcgtt caaattcgtt
    62041 gcttttgctg ctttaaccat ttcactgcgc aaatatgtgc ttgcttcata atatagaggc
    62101 attcctggta attgtcctgg gttgtagcca aatcctgttg catctgcatc aaaataagct
    62161 actttgtctg atataacgat gtcacctact tgtaagcctt caccgattcc gccagctgaa
    62221 cctgtattga tcaccatgtt cacgccgtat tgttggatca acagacttgt ggtaatagat
    62281 gctaagactt ttcctatacc ggaacgtacg acgatcactt cgtggttacc taatgaacct
    62341 gaaataaata aagcgcctgc gcgttcccat gataatggtt cgcttaggtt ctctctcaaa
    62401 atttttactt cttcttccat tgcgccgata ataccgattt tcatcttttt aattccttct
    62461 ttctatataa atgcgattgc taaaaaaacc acacataaca ttaggataac aataataaga
    62521 gatttcatca aaaaagagtt ccatttggtc gtcttttccc gttcgctgat ccttgttttc
    62581 gtgattggtt tattcttttt gctttcttta cgataaaagc tggcaatctt tttttcttct
    62641 tgctgataag ctgtttctgc ttttcgctgt ttttttaatg actcgcttgc ttgtgcttgt
    62701 tgtcgcttgc gaagttcgct tcgcgtaata agcggtcctt tggccataag ccgatccctc
    62761 tactttcctt tggtcaatag ttcccagtat tgaatcgcat aaatcgtctt tgcatcacaa
    62821 atcaactgat ctttcatcgc ttgttgcgcc tcttcaagag ttagatgata caactccagt
    62881 acttcgtctt catcttgtgc taaaggattc tcgaccttct ctaccccttg tgcatgataa
    62941 atatgaagga cttcatttgc aaaacctggt gaaagataca tcgaagtaag atggctcagc
    63001 gacttcgcac gatagccagt ttcttcttct aattctctag ctgctgtcat ttcgggattt
    63061 tgtccttctc ctggatcgat ttttccagct ggaatctcca aaatcacttt ttctaaaggt
    63121 tttcgaaatt gtttgacaag aagcagacga tcttgttcgt caaaagcaat gatcccgaca
    63181 ccgccaggat gaaataccag ttctctcttc gaagttcctc catctggcaa acgaacttcg
    63241 tctacagcaa catcaataat ttttccgcta tagatttctt tgcgactgat tgttttttct
    63301 tcaaattgct cattgttcag catatcttct cctgctttct ttgtaatcat aactgctttt
    63361 ttttcatttt tcaatttttc tcaaaaactc cttattagtt taccatagat aaaccttaga
    63421 ttttctctaa atatagaaaa tccttcttta gtccgatact ctttatagta gtccgtatgc
    63481 taaaataagt atgtaatcga ataacgaaaa gaattcaata ctcaacgata aatgacatgg
    63541 agggtttatt atgataaaac gaaattggcc gtggcttcta acattggtct tgctatttct
    63601 ctttgtccga aacaatagtt tttcttttgt tctaggactt ttattgatag gtgtagtttt
    63661 agttttgtgg ggactctttg gttcgcgcag gaaaaaggga aaacaagaag aaattcctgc
    63721 actgactgac gaacttgaaa ctcactatga aaaaagcggt atgacagcga atgaaatttc
    63781 attttttcga caaacaatga atcaaacaaa aaatgagatc aatcaattgc aaatgaatat
    63841 gcagcagaca gccaaactta aagcaatcga tctccgccat gatccagtca aagcagcgaa
    63901 agctttattc aaagaattgg tgaaagaacc aaaccgactg catgaagcaa gtcagtttct
    63961 ttatacgcat ctgccgaatc tagttgattt gacaaataag tataatgaga tcaatgacca
    64021 tgagatcaaa aacaaacaga cctatgaaaa actagaagaa agcgcgcaga tcatcgacca
    64081 gctgtcagct ttaatcgcac aagattatca aaaattcgtt gctgaggatc tagatgatat
    64141 cgacgtagag atatctgttg cgaaacagag cttaaagcgt gataataaat atgaaccaga
    64201 acaaaaggat taactctttt gttcataaaa gaagactcgg aacattcaga tgatgtctgg
    64261 ctgtttcgag tactttttct aaagtagaga cattcatgta agaatcgaga acagattcaa
    64321 caaggaggaa ttttttatgg atcataaacc gactgaaaca aaagatgtta caccagttaa
    64381 cgatacactt gaagatttat tgaacaaccc gttttcaact ccaacagata cgctgaccac
    64441 ttcacaacaa gcggagattg atcagctcca agagcaacag acagctgctc gtttaattga
    64501 caaactaccg gcggaacgcc aagaacaagc taaacagcta gcagcaaaaa tcgatgctaa
    64561 tgatgcacag tctgtcatca gctacggatc ggctgcccaa gcaaaattaa gtgaattctc
    64621 acaatcgatg ttgaatcatg tgcaagcaca agatattggt cctgtaggag attccttaac
    64681 tgaactgatg tatcgtctcc aagaagccaa tcctgacgaa ctgcgtgccg gagaagggaa
    64741 catcttccag cgtgtcttcg ggaaggtcaa acaatcgatc tatgaagtga cagcaaaata
    64801 tcaaaagatt ggtgcacaaa ttgacaagat ttcagtgaaa ttagataaag aaaaagacgg
    64861 tctgctaaaa gataatctga tgttagaaca gctttaccag aaaaacaaag actactttga
    64921 tgcactgaat atttatatcg ctgctggaga attgaaaatg gaagaacttc aaacaacgat
    64981 cattccagaa gcgatgaaaa gagcagaaga aactggcgat cagatggatg ttcagattgc
    65041 caatgattac acgcaattcc tagatcgtct ggataaacgt acgcatgatc ttcgcttagc
    65101 tagacagatc acgatccagc aagcaccaca aatccgtttg atccaaaaca caaatcaggc
    65161 cttggctgaa aagatccaag catctatcgc tacagccatt ccgctgtgga aaaaccaagt
    65221 cgtgatcgca tttaccttgc ttcgtcaaaa agatgcagta actgctcaac gccaagtctc
    65281 tgaaacaaca aacgacttat tgaagaaaaa ttcagaaatg ctgaaaatct cagccatcga
    65341 aacagcgaaa gaaaatgaac gtggaatcgt ggatatcgag acccttcaaa ccactcaaaa
    65401 tgatttgatc gaaacaatcc aagagacatt gcgtatccaa aaagaaggaa aagaaaaacg
    65461 tcgtcatgca gaagtagagc ttggacacat ggaagaagat ctgaaacaaa aactgttgga
    65521 tttaacccaa tagatcacct ttcgcttgtt actaaaacaa acgcccaaca acagtttatt
    65581 acaactgttg atgggcgttt ttcttatctt tcttctaact tctccattgg agtctatttc
    65641 tcattcatct tgctgtgtcc atagccatag ccaaaataaa tgatcagtcc aataaccatt
    65701 acgataccga acacgatcca tgtcacagtt tgcagacgaa gcatcaatac aatacaagtc
    65761 aagatggaga cgattggcaa tagcggaacg aatggcactt tgaattctcc agcttttggt
    65821 ttgccaaaca tttttctcaa cttcagtaag cctagactaa caagcatcaa ataggcgagc
    65881 gccatgatat tcgttagttc cgctaataca ttcaatggga aaaatccggc aaagaataca
    65941 gctgccacac cagcggcata agtcgctttt ttcggtgtac gatgcttttg atcgatttct
    66001 gtcataaact taggaagtaa gccatccttg ctgacagcaa acagtaaacg agcaagtgaa
    66061 tacatcatcg agatcgttac cgtcagtagc gttaaaattg cccctacagc gatcaccgcg
    66121 cccactgttc catgtcctac atacctcatt gcaaaagcca ctgggtcatt gactcctagt
    66181 tcagtatacg gaacgattcc tgtcaagatc agcgtcacga tgatgtaaag gacagtagca
    66241 atcagtatcg aaccaatgat tcctcttggc acatcttttt gcgggttttt gacttcctcg
    66301 gctgccattg caacggcatc aaatcccaag aaagcaaaaa acacaagagc tgcaccatct
    66361 agcacaccgg aaaaaccaaa aggcatgaat ggctgccagt tatcaggttt aacgaaaaat
    66421 attcctacaa tgataaacaa cgcgatgata ccaaatttga catataccat ggcattattc
    66481 aaacgtaatg ctttcttggc ttcaagcgac acgatgtaag tgacgaatat cactacgagc
    66541 accgcaatca agtcaatgta tgtaccattt tccggattgt acccgccagt taatgcttgt
    66601 ggaagttcgg cccctaaact tttcaaaaaa ccttgcatat agccagacca tccggaagca
    66661 acagaggaaa ccgctagtaa aaattccccg atcaacaacc accctgtcag ccaggcagca
    66721 aattcaccaa atacaacata cagataggca tatggcccac caatcactgg cactcgcgaa
    66781 gcaaattctg cgaaacttag tccagacaaa atgacaacga ttgcagctaa gacaaatgaa
    66841 agtgacagtg ctggaccagc attttgattg gccgcaaccc ctgtcaccac aaatattccc
    66901 gtaccgatga tcgcaccaat tccaagcatg atcaaatcag acgtttttag ttcttttttc
    66961 atctcactcg tatgattgag attgatctct ttcttacgaa acaaatccat tgaagttcct
    67021 ccccatttta ggttggaaaa cagcgattaa actgttttca tataccctaa aaggatacca
    67081 cctaaaatga ctaaaaggca accaacaata acataaatga gctctttttt cgtttttctt
    67141 tcgccaagga tgaaaatccc acctaaagta gagataatga tccccatttg tgaaagtgag
    67201 aagccaacag ctaaaccaag tgatttgact gttagaagca tgcagatgtt tcctaagccc
    67261 cataataatc cacagatcat gtttttccac acaaactgat cgactttaac tttacggaaa
    67321 gcaaagaagc ttgcgcctaa gatcattccg atactttgag gtaagataat tgacatcgca
    67381 tccaaattcg cccagttgac gatgatggta tatacaccat aaccaatcgt agagaagatc
    67441 aatgctctaa aacccttacc aaaatcaagc attttattat ctgtatctat ttttccttcg
    67501 tcatcttgtc gggcagtcaa atacgcaccg cttacaagaa gagctaaagc aataataccg
    67561 tatacataat ctttcgtttg tgtccattca tggaagaaaa cggctcccgc aatcgtattc
    67621 aaaattaatt gaaaaccagt agacaatggt agccctacag aaacacccat gtacttcatt
    67681 ccatggaact gtccattttg tccaacactc caagctaagc cggataaaaa tccgatcaag
    67741 aaaatccaag gggtgattac aggactaaca acgaaaaaga caactaaaga aaacagaaat
    67801 gcaccgatcg tcattcctag tgtttgttga ttggcatcgc caccaatttt accacttact
    67861 aaaccaatac taccccatgc caacataggg actaatgcta ttaaaatttc cataaccaat
    67921 ctccttttat ttcacatgat aagaaactta tcagcgaaaa cgttaacaac acaagcactt
    67981 tttttcgttt ctaacgattt tctaacgttt ttcattataa aaatttaaga ttgggaaaaa
    68041 taaagtcatt gttgtccctt ttccttcagt tgattcagcc gtcaaatgga tacctaattt
    68101 ttcggccaga ttttttgcta aataaagtcc taatcctgtt gaatgtgttt tgctaagtcg
    68161 accatttctc ccagtaaatc ccttatcaaa gatccgtttc aagtcttccg ctgggatacc
    68221 gatccctgaa tctttaactg aaaggctgac tccttgcgct tccttttcta tgctcacaat
    68281 gatgtttccg tgatccggcg tatatttgat ggcattacta agcaattggt tgaagataaa
    68341 agcgatccat ttagcatcgg tcaaaacagt atgatcctca ccaacgatct cgtagcgaat
    68401 atttttttgg ataaaataat ttgcctgact gcgtatcaca ggctgaatga ttccttttag
    68461 tccatattct tggatgaggt agtcccgcga gaaactgtcg agtcttgcat aatacaaaac
    68521 ttgttctacg tattcatcga tttttgataa ttcattttct agtaatgtat acttttgatc
    68581 gtcaatatcg aactcgatcg aacgaactag caatttagaa gcggaaagtg gcactttgat
    68641 ttcatggacc catgagtcaa tataatcttt ttggtcttgt tgattactga ttgctgattg
    68701 cattacttct tggtgttcac ggatcagctg attgatatat tcttctacca gtttttcttc
    68761 ttctgtcctc ccaccactca aataattttg caaaggggaa gttttttgta tgtcggctaa
    68821 ttttccccac catcttctct tgctcaagta accacccagc agataaaaaa tcagaaagac
    68881 gccctcaatc aaagctaaat aagcaattgt gctcaaatca actttgatgt ttggcgccag
    68941 ccacatcaca aagatcgtca atgcaataaa aaacagccat cccaagatca atgtccactg
    69001 atctttcaaa tatttaaaaa aattcatctt aaccgctcat cctttcaggg aacgatatat
    69061 ccctgtccta cttttgtttc gatatatcct tggatacctg cctgttcgat ttttcggcgt
    69121 aaccgattga tgttgactgt caatgtattg tcatctacaa atcgttcgtc atcccacagt
    69181 gctcgcagca atttttcccg acttaagatc tttccattgt gcttcatcaa aataaataat
    69241 agacgatatt catttttgct caaatcaatg atctctccgt tgatttccat acttccgttg
    69301 tcaacattca acgtaatgcc attgtggctc atcatttcac tagataattc tgtgtagttg
    69361 taagaacggc gaagtaatgc attgattttt gcaatcagta catcgatttg gaaatgtttt
    69421 gtcacaaaat catctgcccc catgttcata gccatgatca tatccatatt cgtattcctg
    69481 ctggagataa aaatgatcgg tactttcgat acttcacgta ttttttgaca ccaataatag
    69541 ccatccaaaa cgggaagatt aatatctagt aatacaagct gtggttcttc tctttcaaag
    69601 tctgagaaca cttgattgaa atcggtcgtt ccaaaggttt caaattgcca tttctcaagt
    69661 tcctccatga tcaattgtcg aatgatctct tcgtcttcga ctaccatgat ttttgccata
    69721 aaaaatccct ccatctttct tcttgctact acaatatcaa agaagccctt gtgaaacaag
    69781 ttggcttacc tggaaaatgt cattttagca aacgttaaaa aaaactggaa atatgcgatt
    69841 taatcgcgtt tgtctcttcc aagcagctta aatttattat gttattctta ataattggtc
    69901 tattatctgt tatttttctt tcgttgtttt tgtcttttaa attttcgata acaccaataa
    69961 ccgaaaagcg ttagcactag caatatacct agaaggagta taagtaatcg ataaagatgg
    70021 tatttttttg tctgttctat ctttgaagag acttcttctg taaagggaac tcgtcttcct
    70081 gtcactagta atcggtgagt gttcacggtg taaggtgtac aagtgataag tgtgactaga
    70141 tcctgctttt ctctaattgc taatgtattt gtttttgttg gaaggacaac aattttctca
    70201 acgacttcat aagcgagcat ccgaccatac acttcaagat agaatcggtc atcttgctcc
    70261 atttcttcaa gatccgtaaa gagttttttc tcagcaatac cgctatgggc ggtaatcacg
    70321 gaatgtgtac tctctcctcc agttggaaaa gaggtgcctt gcaggactgt cgctcctcta
    70381 tctaacaaaa gatcattcgt ttcatcaaat aaaggcaagc taacattgat tttcggaata
    70441 tagatggctc cgatcatatg ttgttcgtaa tattcttttc ctggattttg gacatttcta
    70501 accgcttgct caaaaggatc tttgacttgt cccattcctg ggattactga tgtatgagca
    70561 agcgtctgat ttttcttttc atgcactgct aattgttgct catgtttttc ttctttatcg
    70621 cgttcaagct gcttttgata attctcgatc cttctttgat ccaaataatt gctgagagca
    70681 tctgctaaaa atggatatgt aaaaactgca gctccggcaa gaaacatcac tgccataaat
    70741 aattgtaata aaagactgat ttgggtttga ggtctattac ctattttctt tttcattttc
    70801 ttcaccttct tatttaaaaa aactgcagca acctttataa aaccgattgc tgcagcttat
    70861 ttttgttgct tatacttcag acttcatttt agatttacgg taccagctgt acgctccaat
    70921 catcaaactt attccgatta gtaagaatgc caagagcccg ttcccaccag tagcaggaag
    70981 tgacccgcgt ttcatattcg gtactctttc tggccctgga ttttctactc ctacgatctg
    71041 aatttcttca ttatagcttc cataagagat gataaattca gtaaaggctg cctcacctgt
    71101 tggaagtaca taaccttctg gcgccatggt ctctttcatt tgataggttc cataatctaa
    71161 cccttgaacc tcaaagtatc catttccatc tgcgacaaat tctgttgctt gtccgcttgt
    71221 tgcccactca attctatctg ctcctgattc ataaacagct aaaggcgact cccctttata
    71281 aaatattgcg tattcagaac ggtcttcatt ccacaattcg aatcttgcat ctttaagcaa
    71341 ttcactgctt gatgcatcat atttttcaaa ctttcttcca cctgttgtga ctttctctgg
    71401 cggctctact ccttcttcat aatcaaattc atcatttcgt ccgatttcaa taaccgctgt
    71461 gttgttatgg aaatcatccg cttctagttc tgtggaaacc ttcaaattat acgtgatcgt
    71521 taaatctcct ccacgtgcat ctgctaatct acctaaagta tctactttgg ctactccatt
    71581 ttcttctgtc aaaatcgtca cgacaaaccc gttgcttact acttctacta tataatcttg
    71641 tccttttgtt aaaatcgtct cgccggctcg cacttctatt tcctgatcat aataagttag
    71701 accaactgta ggcgtatctg tgattttgta atttttgata acagcgcttc cgttttcaag
    71761 gacagaatcg atgaaataag gaataggtgc tgtgatcgtg taagaagccg tttttcctct
    71821 ttctagatca atataagaaa tcgtttcttg ttctccagct tcattaataa tcgttacttg
    71881 tctaaggtcg ctttcctcta cattcatctg tttatctgct tctctgattt cgtttttcgg
    71941 atacaaatgc acatctgtat tgtatgtgtc tttccatgta gaaccatccc acacgccatc
    72001 caccatatca ggcatcatca ctggcatcat aacaaccatt ggagacgcga ttctggaaat
    72061 atgagctggc gaataagttt ctaagaaaag atagacttta tctctagaag aaggtgcttc
    72121 ttccgtcact tggacggtag agaataccgc ttctccagct tcattcgttg tttgtttgtc
    72181 aatacttgtc agtcgatatt ttaaaaacca actttctgtg tgccgttttt gaatccattc
    72241 aatcgcttcg gcagaattaa gaccgtcttc tcgttccggt gttaaaggat tatctcgaat
    72301 caaatcgtaa tatacttccg ttacgtcaaa caaatcaaaa tcgactcctg gaatattttc
    72361 ttcaccaaaa tccgtagaaa ccaatccgct attcggtcgt tcttccggaa cagatgagaa
    72421 accttttttg tgtaatgtaa tcgtcacttc acttggtttt tgtgcgactt cttcggcttt
    72481 gactagtttt ccgttcccga aaatccctat tcccgcagta acgagtaaca gtacactcat
    72541 tcccgtgaca atcatttttt tattcatttt tttctccccg ttcttttttt attaataaaa
    72601 cagcacctaa caataaaaga attcctagta aagaaatcaa cgttcttgct tcattcgttt
    72661 gcgggaatac ggaccatttt cctgattgac ctggattgct tggaccactc ggctctttcg
    72721 gtttgtccgt tgtttctttt acggtctcgt tataaacacg tggtgttgcg gattcatagg
    72781 cagagactgg aactttcccg gattctaatt cagccatctc ctgtccgtta actactacat
    72841 attgtggttg gccttttgca tccacccaaa attcagggat gatgacctcg attctcttac
    72901 ttgcttcatc catctcgtaa ccttccacac cttttaattc ttcaaaataa taggtgccag
    72961 aaggcaagaa acgttgtcct gtcgtaacaa ggccctcttt atccgaaatg aatttgctta
    73021 catttttgtc attcaaagga tcattgtctg ctggtttgat ccattgattt ttcaagtcat
    73081 tagcaggagc catatctaaa taatatttcc ccccttctat catctgatat agagcaaaaa
    73141 cagcaccttc taatggtttt cctttttcag ttgttcctct ttgacgtcca tacttgaaga
    73201 agtatggatc tcttagataa ccgacatttt taggatagat atgaatctct tgcagttcct
    73261 cggttgattc tgtcgggtga taaattggca aaatagcagc aattgggata gctgtctgat
    73321 cgatatcaac atccaatcca acttctggat caatcgattc ttcgaaaatc aaataaactg
    73381 catcccgatc ttggtttttc ttaggtgctt tgatacgtgc aatcccgtct cctaatccgc
    73441 caaccgtatc tttcccagtt gttaccgaag tccctatttg gacaagcctt tcttctgtga
    73501 ttagctgacg gatattgttt ctgttcgttt cagcgatttg ctggacgaat tctttaggag
    73561 tcatgttttc agcagctttt ttttcatgat aataatcggt catgtcgtaa atggcaaatg
    73621 tcgctccatt tagtggcacc gtctgtgaaa tgatatctgc tgtttcccct gcttcctcag
    73681 agatagccaa gccatcattt tcatggtatt caaattgatc gttataatcg atatctcgaa
    73741 tcatccgttt atgcaacacg aattccacga catcttcccc tgattcttct gccgacatcg
    73801 catgtggaag aaggactgct gcaagcggta gtaaaacaag taattgaagc atcaaacaca
    73861 acagcttttt cattaccttg ctccctcctt tttagattgt ctgacaaaca aaccataggc
    73921 tgctcctgca agcgccatga agaatgcagc aagcaggata tagaataatc gtccggatcc
    73981 tcctgtagca ggcaacggat tttcaggatc gttcccgaca ctaagctgta atctgattcg
    74041 atttgtctcc aaaagttcat attctaactc ttgctgtaga tcaggcgtct ctgcttcttc
    74101 gtttggataa tacgaaatct caacttttag tgtacccgct tcttttcctg gaactacccg
    74161 atcattttct atgtcaggtt cttctcgata actaatttct aaaataaagt aacctggaag
    74221 ttttctatag ctgtcaggtg acttcacttc cttgatccga tagacccctt ccaacaagtc
    74281 tgaagcattg aatagggaag aatcgtttgg atctggtgcc aagcctccct cgatgatttc
    74341 atactctcct gcactatttt gtttctcaat tgaaaattct gcgccttcaa gacgtcggct
    74401 attcatactg tcaattttca aaagttccaa atcgattggc gctagttcat cttctaaggt
    74461 tacttgtagt acaccatcga tgatttcata gccttccggc aagggagcag tttcgtcttc
    74521 gatgaccgta tcttcaatga taaacttacc ttcttcattc agtgcaaact ggaacacttg
    74581 atcagatagc cgatacccat ttggtgcgac agattccgag acctcatatt caccaataga
    74641 cagatgcaga ggttggtcgt aatttaaatc gacatacttt tctactccta cggcacgaga
    74701 atagaagtat ccgatcccag gcaaacgttt cgattcttct ggttctaacg gattaccagt
    74761 gtatgctcga tacagcggga aggaagaatc ccccactttc ctgatatcga aacctgcacc
    74821 atctaatgta tggccttcat ggtctaattt tctaactcgg atctcccgat aatcaaacgt
    74881 attgtaagca gtgaatgcta cccataaacg atcctcttca tctgttgtat acgtgagata
    74941 ttgccaattt tcttcacctg tttgcttgag ttggatagtc cctatagcat tatcgtaaag
    75001 tcgcaagatc catgtgtcat tttcatcagg ttctttgaaa ccttctgggt tctggctctc
    75061 aatgattttg tattctgcat agttggtagt ttcttcacta tccttataaa caaatgaatc
    75121 tggctctccg attggatgaa taactttctc accatccacg attacatatt tcccgtcttc
    75181 atctactgaa tagaacgtga gtctcccttt ctcttcctct acttcgttca caccagaaag
    75241 ccctttcgct actggatatc cgatttccag tccgttacca tctgtttcat aaagttcaaa
    75301 ctctgcgcca ttgattggca tattactgcc ttcaattagt ttattgatat tcatgtggat
    75361 tggcttgaat tcatttcgca tttccagttt gacatacaac tcatcatact ctgtgccttc
    75421 tatgttaggc tcttgtacaa cagaaagctt gtgatattct ccttcataat aggaattcgg
    75481 atctttacct tccggtccgc cgactctcga accagatgta tattcatcta caagctcgta
    75541 gtgacctaac atctcatctt cgttggctgc atattttttg atttggaatt gatacgtctc
    75601 atctactaac tgataacgtg gtggtccagc gatctctctg atccaaaata tgtcgctgat
    75661 tgctccagtt tcatctccta aaatcggcat cttcgtttca aagatcgcta ttccgtccgt
    75721 ggtagtttta cttgtttcga ttggttcggc ttccccactc tctggatttt ggtcatataa
    75781 tgcaaattcg actccagcca acggctcgcc attttgaccc ttcttgctga cttggaaatt
    75841 acggccttta tgttcgtaac gaatggaaga agatgggaaa tacatccgtc cagtagtgat
    75901 atcattctca taatcctcca ggttctgaat ataggccata ttgttcactg tgcaaaaatt
    75961 tccgtcttga gctcccgcat caagttttac ttggtacttg atcctgacca tttccccttc
    76021 acctaaagaa atcggagaaa atttataagc ttggttttcc cgtccgacat acgttgtcgg
    76081 agtcgccact acttctggtg gggcttgtcc atgacgttct tcatcccata tttctggctc
    76141 ttggtacttc atgccgtttt ctcctggatc tagatggtaa aactgaatat ctgccgatct
    76201 gccagctcca aatggtgaga cattaaaacc agtttcatca taaagtgtgg cttgctggat
    76261 cgttctcttg aaggtagaag agatatcctc caaggctgta acaatgtcat tcttgttgtt
    76321 cgcattgtaa tatcgcggtg ttacgccatc ttcttctaaa tcagcaatat ttttcaatac
    76381 atttcgtcca atagcatcta gtcgttgtct cccgctaacg tttccatcga ttcctaaacc
    76441 aatggagaag atacttggcg ggttctcttg atacttgttt ttcagccaat gatgataagc
    76501 aactgtgttg atcgcagatg acttttcttg cgaagtagga ctgccattcg tattgttatt
    76561 agaagaacca tcaccaaccc agtgagtcgc tttattatct ccttctttta cctctccata
    76621 acgatagtgt acatttactt catcttctgg acggttaaag tcagatgcat aggtaactgg
    76681 atgagtaaac cctgcattag aagtattccc agaaaactcc tcaaaatttc tgaacaaaac
    76741 gtcattatct tcagcaatct tatcttccat cacagtccag ttgctccaag ctccattagt
    76801 gctcgtacga taacttgact gaatcggtgc ataactgaag gtcggtgtcc catctcccac
    76861 aacgattagg attttttctg gatttctatt ttctccgcca ttgtctttat acaatgtttc
    76921 atagccattt ttaagcccta gtgtaagcgg agttcctccg attggtgtac gatttaatgc
    76981 tgtattctca cttcccgtca aacgattgat atcatttgtc aatggaaaga tgtccgaact
    77041 gatttgctca tggttattga tagctgtact attatggtaa aaattcacca taccgattcg
    77101 aatatccaga ctcgggtcag agagcagatc tttactcatt tcgtttactg cctcaatcaa
    77161 cgcagcattt ttagtttgac tattggtccc agcggttaat tcactcatac tcgctgactt
    77221 atctagaaca aaaacgatat cgattggact gatttccttt ttctcgcccc cgatgacatc
    77281 tagatagaca ttgtaaaggg ttgttgcatc ttcaggatgt agatcagatg ctgtgctgct
    77341 tggcagaaca ggttctaccc attttttgat gtacgccccg ttataatcca agtaactgtt
    77401 ttcaaaattc cgttcatttc cccaaagctg aacggtttga gctggaacgt ttctatcttc
    77461 ttcgcctgca gttgatccat tattagtagc atagtcgtaa tttcgccagt tcgctccccc
    77521 gtttgtataa tcactatcat cgatcaagtt ctctccgtca tatggcgagc ctttgtctcc
    77581 ttgatagcgt tcattgcttt gtgtccaatt tcctaacact tttgtatagt cattcgttgt
    77641 aaacttcatc tattctttca tcaaccacca cttgaagagg tagttaccct tcgttcacat
    77701 cggggatctt ttctgtttca aaaatcaatg tgccggtttc tgcttctttt gaataaactt
    77761 ttccgacata ccattcttga ccatcgatcg tctcgacttt taggtcttct ccatcttcat
    77821 cataggagga tagacctgtt ccacgaacgt tttttacggt accaatcccg gcggctgccg
    77881 catcgattgc taaacgtatt ctaccttcat tggcactttc ctttttttca aaagccagat
    77941 gccattcaag ggtttcttct ttttcaatat acttcccttc aatttttgca tagtcctcat
    78001 ctaagaatac ctcatcctcc tttgtttctg ttgactcagc agctacatcc agggtcgtca
    78061 tatgatagac tgactggaaa tatggtccaa atagcaaact gacagttact aactgcaaaa
    78121 gcttcaattt taaccctttc acatccttat actcctttca aaaatatttg tcttctttca
    78181 caacaatcaa caaaacaatg cccatctaat tataatttaa tttaatttta attttaaatc
    78241 aaatgatatc ctttttagtt aaaaaacacc aattatacaa catttattat taaaattaaa
    78301 ttataaaatt ttataacgtt ctcatttaac aaaacttaag ttattaagac aaatgaataa
    78361 acaactacgc tttcctcgaa acaaaaaaat aaagcacaaa tgcgcattta cgcactcatg
    78421 ctttattcgt acaattaatt cagataacgg cagcgcatag gatgccaata acacttccta
    78481 aaatagctcc tcctaaaatg tcagtaggat aatgtaaaag taaaaaaatc ctcgaataag
    78541 cgatcaaaca agcaaataaa aaggctaata ttcctgcaac tggacttaat tcgaacaacg
    78601 cagctactga taaaaaggct agcgctgtat gtcctgaagg aaaagaagaa gtcttcggtt
    78661 tacttgtgta aaggacaagc cgacgatttt tttcataagg tcttttccgt cttgtcgttt
    78721 ttttgataaa aagcaaacca acaaaaaaat agatcaaact gccgataaga atggctagac
    78781 catattctct agtatcaggt atcaagacta aaacagcagc aaggagcaac caaataaaac
    78841 caccattaga gatattactg ataaatttca aaaaattgat ttttcgacta tccgttttcc
    78901 gttgcttggc gaacttgttg ataatactat catcgatttt agcaatcaaa cgcaacatgt
    78961 tattcctcct ttttaagaac cttttcttgc gtacgatgga taaaaagcag gacttgaaaa
    79021 taaaacaaga aactgaaacc taataaagca aaatagattg gtcctgcaaa ccagaatgcg
    79081 acaagaagga tcaacgtgcc tgccaaatag aaaagcgcgc ttttgaaatc attaaaaaac
    79141 aagaaaaatg cagtaatcca agaagcttct ttttgttcca gttgaagcaa gattttgtaa
    79201 ctaaagagaa gcgcgcttaa tagaagcaaa aacaacgtag tatacatcaa tgtatagcca
    79261 attcccccac cccaatagcg gaaaatctga tagtcgatca atagaagaag aaaataaaca
    79321 cttgctaaac tatcttgctt tccataaaga gagaagttct cacgaaactt tttccagaat
    79381 agtgaaccga gctttttctc ttcatttttt ttgtaatgat aaatcatata cagaactgtt
    79441 cgacttgcag ttcctaatgt gaaaacaggc aagacgcaca ggaaccatag tagctgtaaa
    79501 tggataagac gtggaatcca ttcaatgatt tctaagaaat aatcattggc ttttttacca
    79561 atcgatcgat tcatcatcct tttgaacctc ctgtcaaatc catcccttca atgaaataac
    79621 gttgtgcaaa aaagaacagc aaaatcgtcg ggatcaagac aagtgttgct cctgccatca
    79681 gataattcca ttgtgtcgtt gcttggttct gaaaaacttg caagccaatc tgtaacgtgt
    79741 atttttcttg atcttggata tataacagag gacctaagaa atcattccaa gctgcgttga
    79801 aggaattgat cgcaatggta atcaaggcag gtttcgtcaa tggaagcatc aaatgactcc
    79861 aaatatacaa atgattcgct ccgtcgattt cagcagcttc gatcaattca ttattgatcg
    79921 ataagtaaaa ttgacgcatc agaaatatat tgaacgcatt gccgaagaac gatggaacga
    79981 tcaggggcaa ataagtacct acccagccga tcttggagaa taatacatat tgagggatca
    80041 tcgttacaaa ccccgggatc atcatcgtag aaagaaccaa agcaaacatc agctttttcc
    80101 caggaaaatc aagcttcgca aatccataag caataaaaga atttgaaagg acattaccga
    80161 tcactactag cacagtgatg aataatgtat ttcttgcata gcgccaaaaa ggaaaggcgg
    80221 caatcgtatc cagatagttt tcccatacga ttttttctgg gaaaaaggtc ggaggataac
    80281 gaacgatttc ttccattggt ttcaatgaag taaacaccat ccaaagcaat agaatgatca
    80341 aaatgattcc tattactgtc atgaggacat aacttagtat tttttttgtt tgccgattca
    80401 cactggtctt tttgaaataa tccatcattt gccgtcacct ccagaataat agacccactt
    80461 aggtgcaagc ttcaaattga caaacgtcag aaccaacacg atcagaaaca ggatcattga
    80521 catagccatc gcataaccca tatcaaaagc tacgaacgcc ttgttccaca tatgcagatt
    80581 ataaaaaagc aaagaatttg caggtccgcc tgttccattt ttcgtcatga cataagcttc
    80641 ttgaaaaatt tggaaagaac cgatcgtaga ggtgatcaca tcgtaaaaaa taataggagt
    80701 gattaatggc aaagtaatac ttttgaactt tccccaaacg cctgctccat cgatttctgc
    80761 tgattcatat aaagatctag gaacattttg taatgtagct aagtaaagta gcattgctcc
    80821 tccagaactc catattttca tcaaaatcaa cgcaggtttc gtccattgtg gatcaaacag
    80881 ccaagctggt ccagggatat ggaaccagcc cagaaatgtg ttgatcaatc ctgcagatgg
    80941 agaaagcagc tgcatccata agatgtagac tgctactcct gataagactg ccggtaaata
    81001 gaaagctgta cggaatacac tgattccttt gattttttga ttgagtactg tggctaaaaa
    81061 cacactgaaa atcgtcgtca gcggtacatt gaagatgacg aaataaacgg tgttgtataa
    81121 cgatttccaa aataaatcat cgtgaacgaa cattcttttg aaatttgcta gaccaatgaa
    81181 attcatcttg gatgtcaaat tataatcggt gaaactacca ataactgaaa aaagcatagg
    81241 ccctagcata aataccaaga acccgataat aaaaggcgag ataaataaat aggcccatct
    81301 tgcttctttt ttctttcgga tagataaacg cttcaaattt tcttgcctcc tctgtaccta
    81361 gacagtcaaa aatggattat tgattttgct tgactaagtt ttctactgct ttttgtgcat
    81421 ctgccaatcc ttcttttggt gattttgttc ctaaaagaat ctggtcaatc tgttcattca
    81481 ctaatgtcgt gtaatccgga gcatctaaag gaactggtgt gatcaccgta tttttaaagt
    81541 tttcgtccgc catttgatag atcatttttc ctttatccgt taggttgtca ttattcacta
    81601 aattctcatt ggctgtcttg ctcgccatga tatcaaaact tttttcgccg aatttttctt
    81661 gtacttctgg agaagaaaga tatttgatga attcataaga ggcctcagga tcttttgctc
    81721 cgtatggtac ttccaagacg aatccgccac cccacgacca atggcctgaa ccttcttctt
    81781 tttctgggag ctgcatcacg ccaaaatcaa aatcatccgg tgcattttcc atcaaactag
    81841 agtaataatt gatattttgg gcacgcatgg ctactaatcc ggaaataaat ggatcagata
    81901 caccagaccc aaattcggct tctaatttat tgatcgtatt tttcccataa taatcttgcc
    81961 agtcttggat ccactccaag gcttccactt tatttttagt atcaatcttg actttttcat
    82021 ctttgtcaaa ccaactagtc ccgttatctg cgtttagcgc ccatacatca gcccctaaat
    82081 tccataatgg ataaaaaccg atgcgtgtaa attcttttcc ttctttttta tctagtttac
    82141 gtgctgtttc ttctaattca tcccaagttg tcggtagatc ttgttcactg attccagctt
    82201 ctttaaataa ctttttatta taaaaaatga cttgtgtatc tgtattgaat gggatcgcat
    82261 aaggatcacc ttcatattct actgtatccc aaagctgcgg ataaaaatcc ttagaagtgt
    82321 ctttttctac atatttggag agattagttg cttgctgtgc ttctgctctt tgggcaacgg
    82381 aatttatgtc ctgtacaata acatcgggtg catttccagc tgttattgca gataaagatt
    82441 tagtccagat gtcaccccat ggctgatact tataatttac atggatctcg ttttgtgatt
    82501 gattgaaatc ctcaatgatt tcttcgatag cttcttttcg tgcaccggat ccccaaaagg
    82561 accagaaatc aatatttttt cttccatcac ttgttttttc agaagaattt gctgcacttg
    82621 aactggcatc gcttgcatta cttgatccgc atcctcctaa taaaaacaat gctgaacttg
    82681 ccagcaaccc tgtaaacaat aattttttca tattttacct ccaaatttta atgtaaccga
    82741 tgccggtttt aatataacga tatcattttg ttctgaaaat aacaatttat aaggtatgat
    82801 tctaaaaaaa ttattaatga taccaatact ttatataatt cgcgagcgct ttctttgaat
    82861 tttttaatct ttcgtagcag aatgagaatg tattaaaaat agattaggag gaattttgat
    82921 gtttagacac cagaaagaat tgcagtttga agcaaaacca gatcgtccga atccattaat
    82981 cgcgaaatat ctgcaggagc tgatcggcgg ccaatatggc gagatgagtg tagctatgca
    83041 gtacctgttc caaggctgga gctgccgagg agaagaaaaa tataaagata tgctgatgga
    83101 cattgcaaca gaagaaatcg gccacgtgga aatgttggcg acaatggttg ctcgtttatt
    83161 agagggtgcg cctgttgaag atcaagaagc agcgatgcaa aaagatccaa caatcggtgc
    83221 cattatctct ggaatgaatc cacagcacgc gatcgtagct ggcttaggac ctcgtcctgc
    83281 tgacagcgtg ggcaatccat ggagcggtgc ctatatcatt gcgagtggca acttgttagc
    83341 agactttaga gctaatttaa atgccgaatc acaaggaaga cttcaagtag cacgtattta
    83401 cgaaatgatc gatgatcgtg gcgtaaaaga tatgctgtct gttctccttg caagagatag
    83461 ctatcatcaa aatctatggg aatgtgcagt gaaagaatta gaagaaaaag aaggttcaat
    83521 cttagtccca agtactttcc cgcgtgacaa agaacgaaaa gacattgctt atgacttcta
    83581 taacttctca gagggagacc aaagtagtga aggttcttgg gctagtggca aagcactgga
    83641 tggcgaaggc gaatatcaat ggttgaaaga acctaagatc gaaggcgctg caccaaaatt
    83701 agaaccagta acaccaaaaa tgtatgggac acctccattc aagaaataaa tatctacccg
    83761 tagaaacacc cgttttttgt tccacacgct ttgtgagcaa aactgactaa agtcattaat
    83821 cataaccacc cttcaaaagg gtggttttct ctagagctat aagctcaaat caccggccag
    83881 cgcctaaaga cgctggcttt catattttat gttcaagcca tattgccttt gccagttttg
    83941 cccacctctt aaaaggttta tgtactactc cttagccctt aaaaggatcg tcttactctc
    84001 gtacactcag cttatctaac gcactatcat gtttttcttg ttcttgaatg tactttttga
    84061 tggtcgcttc attttgtcct acttttgtca catagtatcc ttccacccaa aagtgtcgat
    84121 acccaaattt atattttaga tttgcacaat ctcttgatga tctcctgaat attggctcga
    84181 tattgattat aaattatttt tcttctatac tttggtgtga atacaatgtg gtatttacac
    84241 atctactttg tacgggctaa actatttccc tttattgctc cacccgcata gcaagtggtt
    84301 ttgtatttca atcgctcggc gcgctcaatt aggtctaaag acaataaatc taaaaacccg
    84361 ctgcttcact gacagaaaca acgggttttc agaaaaaaat gaaatgaaaa agatctctta
    84421 cggcaatcta ttttattttt ttgatttttt ataaatggaa aatgcttcaa gaagtccaag
    84481 aaataaagcc gtataaaaac aagaacctat gaaatcttta tttattagat attcaacaga
    84541 aatcccgata acactagcca acaatatatt tatcagaagt gtaaaataag gatgcttttt
    84601 atagaataga actagtgttg ttttcataga ctctcctttc ttcatatttc caattaatca
    84661 caaattcacg gcattactag aaaaattaaa ggggcgcaga agtggaaaaa tactcttcga
    84721 ctttccctac aaaccaggcc atcttgtgtt taataactat accaaatcta aggtacctct
    84781 aaatctaaat tccatttgtg gcggtgacct ccaattttta actcttctat ctcccatcca
    84841 gtatacataa ccttgatact tcgaccactt atatatcgaa cacacaaata cctatccatt
    84901 gcaggagtac tacctaactt agttgctact tgcatctaaa taacagctga aaagatagga
    84961 cttactctta gtaaagtatc tgaactatta atttttaaac tatcattttt atcctcaact
    85021 acatgcacat ctgttgcaac ttcactttct gtctcatcca gataaatgat taaggaatcg
    85081 ggaattgcaa tatcttcagc tgacgctgga atagttgagc taaattagtc atcatatctt
    85141 tattataaaa taaccaacag caaaaaatac aatgaaaaac aaacttggaa ctaatgtatc
    85201 attaataaat gttgaaaaat tcagcaactc attactgtat gattttagca gcccaaccaa
    85261 catataccct ataaaagcta ataagatggt taggaatcct ttagaatttt t
//
LOCUS       DACKAC010000005        70475 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.005, whole genome shotgun sequence.
ACCESSION   DACKAC010000005 DACKAC010000000
VERSION     DACKAC010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 70475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.005"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(551..2164)
                     /locus_tag="I0B46_000345"
     CDS             complement(551..2164)
                     /locus_tag="I0B46_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAR2082675.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2305..3213)
                     /locus_tag="I0B46_000346"
     CDS             complement(2305..3213)
                     /locus_tag="I0B46_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAR2082676.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            3396..4520
                     /locus_tag="I0B46_000347"
     CDS             3396..4520
                     /locus_tag="I0B46_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAR2082677.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            4747..5664
                     /locus_tag="I0B46_000348"
     CDS             4747..5664
                     /locus_tag="I0B46_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAR2082678.1"
                     /translation="MIQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            5657..6490
                     /locus_tag="I0B46_000349"
     CDS             5657..6490
                     /locus_tag="I0B46_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAR2082679.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            6573..7691
                     /locus_tag="I0B46_000350"
     CDS             6573..7691
                     /locus_tag="I0B46_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAR2082680.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            7685..9583
                     /locus_tag="I0B46_000351"
     CDS             7685..9583
                     /locus_tag="I0B46_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAR2082681.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            9577..10800
                     /locus_tag="I0B46_000352"
     CDS             9577..10800
                     /locus_tag="I0B46_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAR2082682.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(10886..12817)
                     /locus_tag="I0B46_000353"
     CDS             complement(10886..12817)
                     /locus_tag="I0B46_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAR2082683.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARSLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEIAELLAQDEPESAPAPTNKSDYYQSKEQ
                     QKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQDQ
                     ETKLTEWEELSLELEEYEN"
     gene            13043..13699
                     /locus_tag="I0B46_000354"
     CDS             13043..13699
                     /locus_tag="I0B46_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAR2082684.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            complement(13773..14126)
                     /locus_tag="I0B46_000355"
     CDS             complement(13773..14126)
                     /locus_tag="I0B46_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAR2082685.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            14318..15247
                     /locus_tag="I0B46_000356"
     CDS             14318..15247
                     /locus_tag="I0B46_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAR2082686.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            15258..16094
                     /locus_tag="I0B46_000357"
     CDS             15258..16094
                     /locus_tag="I0B46_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAR2082687.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            16342..17118
                     /locus_tag="I0B46_000358"
     CDS             16342..17118
                     /locus_tag="I0B46_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAR2082688.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            17294..18037
                     /locus_tag="I0B46_000359"
     CDS             17294..18037
                     /locus_tag="I0B46_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAR2082689.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            18030..19610
                     /locus_tag="I0B46_000360"
     CDS             18030..19610
                     /locus_tag="I0B46_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAR2082690.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(19861..20340)
                     /locus_tag="I0B46_000361"
     CDS             complement(19861..20340)
                     /locus_tag="I0B46_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAR2082691.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(20356..21108)
                     /locus_tag="I0B46_000362"
     CDS             complement(20356..21108)
                     /locus_tag="I0B46_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAR2082692.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            21225..21295
                     /locus_tag="I0B46_000363"
     tRNA            21225..21295
                     /locus_tag="I0B46_000363"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21257..21259,aa:Gly,seq:ccc)
     gene            21656..22381
                     /locus_tag="I0B46_000364"
     CDS             21656..22381
                     /locus_tag="I0B46_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAR2082693.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            22636..22902
                     /locus_tag="I0B46_000365"
     CDS             22636..22902
                     /locus_tag="I0B46_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAR2082694.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            22899..23330
                     /gene="ruvX"
                     /locus_tag="I0B46_000366"
     CDS             22899..23330
                     /gene="ruvX"
                     /locus_tag="I0B46_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAR2082695.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            23355..23660
                     /locus_tag="I0B46_000367"
     CDS             23355..23660
                     /locus_tag="I0B46_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAR2082696.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            24229..25065
                     /locus_tag="I0B46_000368"
     CDS             24229..25065
                     /locus_tag="I0B46_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAR2082697.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            25130..25564
                     /locus_tag="I0B46_000369"
     CDS             25130..25564
                     /locus_tag="I0B46_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAR2082698.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(25632..26954)
                     /locus_tag="I0B46_000370"
     CDS             complement(25632..26954)
                     /locus_tag="I0B46_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAR2082699.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(27108..28748)
                     /locus_tag="I0B46_000371"
     CDS             complement(27108..28748)
                     /locus_tag="I0B46_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAR2082700.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            29078..29548
                     /locus_tag="I0B46_000372"
     CDS             29078..29548
                     /locus_tag="I0B46_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAR2082701.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            30177..31280
                     /locus_tag="I0B46_000373"
     CDS             30177..31280
                     /locus_tag="I0B46_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAR2082702.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            31564..32244
                     /locus_tag="I0B46_000374"
     CDS             31564..32244
                     /locus_tag="I0B46_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAR2082703.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            32334..33368
                     /locus_tag="I0B46_000375"
     CDS             32334..33368
                     /locus_tag="I0B46_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAR2082704.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            33382..33717
                     /locus_tag="I0B46_000376"
     CDS             33382..33717
                     /locus_tag="I0B46_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2082705.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            33701..36181
                     /locus_tag="I0B46_000377"
     CDS             33701..36181
                     /locus_tag="I0B46_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2082706.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            36237..37796
                     /locus_tag="I0B46_000378"
     CDS             36237..37796
                     /locus_tag="I0B46_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAR2082707.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            37793..39157
                     /locus_tag="I0B46_000379"
     CDS             37793..39157
                     /locus_tag="I0B46_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAR2082708.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            39328..40398
                     /locus_tag="I0B46_000380"
     CDS             39328..40398
                     /locus_tag="I0B46_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAR2082709.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            40413..41081
                     /locus_tag="I0B46_000381"
     CDS             40413..41081
                     /locus_tag="I0B46_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAR2082710.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            41101..41679
                     /locus_tag="I0B46_000382"
     CDS             41101..41679
                     /locus_tag="I0B46_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAR2082711.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            42082..42993
                     /locus_tag="I0B46_000383"
     CDS             42082..42993
                     /locus_tag="I0B46_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAR2082712.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(43249..44166)
                     /locus_tag="I0B46_000384"
     CDS             complement(43249..44166)
                     /locus_tag="I0B46_000384"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAR2082713.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            44368..44805
                     /gene="zapA"
                     /locus_tag="I0B46_000385"
     CDS             44368..44805
                     /gene="zapA"
                     /locus_tag="I0B46_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAR2082714.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            44863..45393
                     /locus_tag="I0B46_000386"
     CDS             44863..45393
                     /locus_tag="I0B46_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010781150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAR2082715.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIMFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELW"
     gene            45525..47885
                     /locus_tag="I0B46_000387"
     CDS             45525..47885
                     /locus_tag="I0B46_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAR2082716.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            47972..48286
                     /gene="trxA"
                     /locus_tag="I0B46_000388"
     CDS             47972..48286
                     /gene="trxA"
                     /locus_tag="I0B46_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAR2082717.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            48468..50246
                     /gene="uvrC"
                     /locus_tag="I0B46_000389"
     CDS             48468..50246
                     /gene="uvrC"
                     /locus_tag="I0B46_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAR2082718.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            50391..51290
                     /locus_tag="I0B46_000390"
     CDS             50391..51290
                     /locus_tag="I0B46_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAR2082719.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            51283..52545
                     /locus_tag="I0B46_000391"
     CDS             51283..52545
                     /locus_tag="I0B46_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAR2082720.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            52708..54606
                     /locus_tag="I0B46_000392"
     CDS             52708..54606
                     /locus_tag="I0B46_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HAR2082721.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKEYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(54905..55624)
                     /locus_tag="I0B46_000393"
     CDS             complement(54905..55624)
                     /locus_tag="I0B46_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAR2082722.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            55961..56293
                     /locus_tag="I0B46_000394"
     CDS             55961..56293
                     /locus_tag="I0B46_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2082723.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            56280..58283
                     /locus_tag="I0B46_000395"
     CDS             56280..58283
                     /locus_tag="I0B46_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAR2082724.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            58302..58772
                     /locus_tag="I0B46_000396"
     CDS             58302..58772
                     /locus_tag="I0B46_000396"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAR2082725.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            58793..59377
                     /locus_tag="I0B46_000397"
     CDS             58793..59377
                     /locus_tag="I0B46_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAR2082726.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            59392..60378
                     /locus_tag="I0B46_000398"
     CDS             59392..60378
                     /locus_tag="I0B46_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAR2082727.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            60368..60679
                     /locus_tag="I0B46_000399"
     CDS             60368..60679
                     /locus_tag="I0B46_000399"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAR2082728.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            60701..62482
                     /locus_tag="I0B46_000400"
     CDS             60701..62482
                     /locus_tag="I0B46_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAR2082729.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            62475..63851
                     /locus_tag="I0B46_000401"
     CDS             62475..63851
                     /locus_tag="I0B46_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAR2082730.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            63858..64493
                     /locus_tag="I0B46_000402"
     CDS             63858..64493
                     /locus_tag="I0B46_000402"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAR2082731.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            64523..65878
                     /locus_tag="I0B46_000403"
     CDS             64523..65878
                     /locus_tag="I0B46_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAR2082732.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(66070..67464)
                     /locus_tag="I0B46_000404"
     CDS             complement(66070..67464)
                     /locus_tag="I0B46_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAR2082733.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            67556..68527
                     /locus_tag="I0B46_000405"
     CDS             67556..68527
                     /locus_tag="I0B46_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAR2082734.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            68524..69306
                     /locus_tag="I0B46_000406"
     CDS             68524..69306
                     /locus_tag="I0B46_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAR2082735.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(69475..69846)
                     /locus_tag="I0B46_000407"
     CDS             complement(69475..69846)
                     /locus_tag="I0B46_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAR2082736.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            69980..70423
                     /locus_tag="I0B46_000408"
     CDS             69980..70423
                     /locus_tag="I0B46_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAR2082737.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
BASE COUNT        23102 a        12015 c        14498 g        20860 t
ORIGIN      
        1 taaataaata ggaacaaaaa aattaaatta tttaatacac acattaggga tcattcattg
       61 aaggattaga agacaacgag gatggcgaaa aatatgatta caatagaaaa gtcgttctaa
      121 accaatcagt tcctagtatc acacaacgca agccagtcga tttggtgaga ttagaggagt
      181 gaaagcaaac tttattatat aagaaaaaat attcgaaagg gatgctagtt taggtactag
      241 tgctgataga ttaatatgat gcctcgtaat attaggcgta gttactctag agacatcact
      301 atggactatg atttgtctca gagtcacaac atgttttttt atgaaaagaa acagctaaaa
      361 taattaaatt gcgcaatgtt taaactttct taaactataa cctaataaat gtgaagaaat
      421 aatgcacaat ttcttcacat ttatcttcta ttctatatac ataccaacca acaaccctat
      481 atactttttc atttttactc ctcaagtaaa aataactcct agaccgcggc tacccaccgc
      541 ggtcgttttt ttattcttca atcaaaatcg ctttgatacc caaacctgcc aagattcggg
      601 cagaagtata agtattcaat cctcttctga aagtaatgta aactttttta tcttttggga
      661 tttcattgat tcgatctcgt aactcattca ttggaatatt ttttgttgcc gagatactgc
      721 cagaaggtgc tttattaggt tcacgtatat ctaaaaagaa cgcttctttc aaatcgtttt
      781 cgggtatatc acttgctttg atcgtttcta cgatattcgt catttgattg attgcgacat
      841 aaccagctat attcaacggg tcacgggtag tagaatacgg tggagagtaa ggcaactcca
      901 aatcaggcaa atcaaagact gtcaagtttc cggtgattgc tacagaaagt tctcccatcc
      961 gtttatcgac acctttttct ccaaatgctt gtccacctaa aatacgaccg ctatctgcat
     1021 caaaaatcag ctttagattt aatcttgtag cttctggata gaaataggca tggtcaaagg
     1081 gagtgataaa aacagtttta taatttgtta tgcctgatgc ttttaattca tgttctgtca
     1141 ttcccgcata acttgctgta tgatcaaaga tttttgcgac acctgcacca atatatccgc
     1201 gatagcgcat aggcgtacca tttaaaatat ctgccagcat atgtccttga cgattggccg
     1261 cactggaaag catactttgg atcggctgac cagtcactac actagtcgtt tcgataatat
     1321 cgccaatcgc atagatgtcc ggcagattgg tttgtaactg gtcatcaaca atgatctgac
     1381 ctgtatctga taattgtatg ccggttgctt tcaccacttc attcttcgga gaaacaccaa
     1441 cagcaaaaat tagcatatca gcagaaagga cggagccatc tgataatact atttctcgac
     1501 ctttatcacg gatctcagta actcttgttt ctaaatgaac ggctaatcct tctttcagca
     1561 atttgtcata aactaaatta gcaatttctg gatcataagg gaaagcgact tgtggcaatt
     1621 gatcgattaa agtcacgtct aagccgcgat gcttgaaatt ctcagctaat tccagaccca
     1681 taacacctgc accaagaatg acgacatgtt gtggattttt ttcttcaagg aacgatttga
     1741 ttcgatccgc atccgtcaca ctacggagga caaagccgtt ttctgcttca aaaactccgg
     1801 gaatatccgg ataatctggt ctagcaccag aagagatgat caacttatcg taatctgttt
     1861 ttatttcttc atttgtggat aggtctttaa ctgttaatcg cttagtagaa ggatcgattg
     1921 ctgttacttc gtgtttagta aagacatcga tgttgttttt tgttttcaat atttctggtg
     1981 tacgttcgat cagcgagtca cggtcagtga tcacacctcc taaataataa ggcaaagcac
     2041 aacttgcata agaaatattt tctccgcgtt catagataat gatttcgtgc gcttcattca
     2101 atcgacggaa acgagtggca aatgaaggac cgccagctac accgccaatg acaacaactt
     2161 tcataaaaac atctcctttt ctacgttgat cgtactatgt tatggtaaaa aacagcaaat
     2221 aattgtgccg gaaaattatt gtttattttg aatagacgga aaatatagag aaataatttg
     2281 tggataaaac ctaaaaattt ttttctattt taaggataga taatactttg aaggggataa
     2341 ccctgtatat tttttaaaag ctgaagaaaa atgatggatc gaatgaaatc ctaatatatc
     2401 tgcaatttct gtataggtta attcattttc tagcacgaga cgttttgctt tttccatttt
     2461 tagttcttta aaatagctaa taacactttt tccattcact tttctaaaat gtttttttaa
     2521 gctggctgga ctgataaaaa aaatttgggc taattgttca agcaaaaaat tttctgaaag
     2581 atgttcttct aaatatcgta tgattggatc aactaattca tctgaataaa gaatatgctt
     2641 ttttttagga atgactttct catttgaagg tattgtcaat aaagcaagtt cctgttgata
     2701 atgccttggt aaggaagaca attgaatttt ttgcagacaa taagtaacta ataaggaaag
     2761 caaatgaatg ctcatctgtt ggcatagtat gaaaaaagaa ttgatttcct gatcatagga
     2821 aatagctggc ttactatgtt ttagataatc aattttgaac tgttcatttt tcagaaaagt
     2881 aatgatagat gttatttctt gatctataga aaaagattgt cccgtaatat gtgctaataa
     2941 ttgctcatca aaataaaact ggataatgat aaaagtttgt gtttcctttt gagtagaaaa
     3001 gatttggact tcttgctgtt tatcaatgaa taaaagggaa ggattatcta gttctataga
     3061 tttatgtgca aattcagcat ataaattagc gctttcattt attagtagtc gaaaataggg
     3121 agcagcagat aaatgaagat gggtatctat ggaataggtt agtgtttttt tgacaagatt
     3181 ggaggattgg ttgtttgtta acaaaagaac cactttcctt tctcttttta tataaaaagc
     3241 tttctattta taattaatga ctattataac aaaaatgaaa agccaaaacg acaagtaaaa
     3301 tattttttta tataaatatt tgtgttacta attccgttaa tatacataag tgaaagcgtt
     3361 ttatataaga acttgaaaag aaagaaggga tagacatgaa aaaaagatac aaattggtga
     3421 tagctttgct aaacattacc ctattattca gcttaagtgc atgtggaaaa actaacgaag
     3481 aagtaacagc aaacatcgca gatccaatga taaaaaaagc cactacatta gaagtatatg
     3541 tcgcaaacgg caaactgaca aactatgttg aaaatgctgc aaaaatctat aaccgcaaaa
     3601 ctggagcaaa tattgactta aaaattgtga atgtagcttc gggaacagca acagtacaaa
     3661 tgatcacccc aaaattggta acacacgaag atatgccgga tattatctcc atggcagaca
     3721 cttcagcagc aggtgtgctg gaaaaatttc aagatgcttt ttatagtgct gatgaatatg
     3781 gattttatga caaatatggc gatcagtttt atgatcaaaa attgaatatt ttgcgtcaac
     3841 aaacgagtaa tgaaacgatt attccttggg ctagtgattt taccccagca ctttgttatt
     3901 atcaaccaaa aaaatttgct gaaataggtg tagattttga agaaatcagt tcttgggaag
     3961 aatatatcca ggtggcaaaa gcattaaaag aaaaaactgg tagttttgga attgccttgc
     4021 cagaatctgg ggaccaagaa ttgtttatca acatgctagc ccagcagaat caatctttgc
     4081 ttgatgaaga aggaaatatc cgtctgaata ctaaagaagc gaaacatgct gcaaagttaa
     4141 tcaaagaaat gatcgactca gatattgtac atttctacgg tgcacaagat gctgagaaag
     4201 catttcaaga atctgcaatg tttgttgctg gcggctggta tgctacgaat atgtctttga
     4261 attttcctga tgcatctgac aaatggcgca tggctccact gattcctttt tcaaaagaaa
     4321 atccaggaaa aggtgcagta tcaggaggat caagttttta tgtccctaaa gatgccaaaa
     4381 atccacaagt ggcacaacaa tttcttacat ttatgctgac agatgaagaa tgcttagcca
     4441 acgcactgga gctgggcgtg gctacatcta actcattggc ctatcaaaca gaagcagctg
     4501 agaaaaagtt tgactactaa ggaggacaaa aatactatga actattgaat aaaatcaatg
     4561 aaaatacatc aaatactgtt ttaccagcca cttattcaga tgctacgtct tatttgacta
     4621 ccgctgcata taactattgg caaacaggtg attttgaaaa ttcatatcta aaagaagcag
     4681 aaaattttgc tcataagtac ggcgtaaaat taactggtga ttaaaaaata ttaggaggac
     4741 ttaacgatga tacaatcttc gttaccaagc aagacaataa agaacagcaa gaaaaaagca
     4801 acagatacgt ctcataaaaa aagtgcttat ggattggtca caccttttgt tattttcttt
     4861 attgtgacga ttttgattcc catcattatc tcgtttgctt ttagttttat gaaaattggc
     4921 tactcatttg aatttgttgg atttgacaat tatctagcta tcttccgaga tccattgttt
     4981 attaaatctt acacgaatgt tttgattatc atggcaggat caattccaat aacgatgcta
     5041 ttagctattt actttgctgt tttattgaat tcagcacagt taaaaggaaa aggatttttt
     5101 agaactgtct attatattcc aaccattaca tcaacggtgg ctgtagcttc cgtattcatg
     5161 acttttttca atccgactgg gttattcaat ggatttttga atgcgctagg aattaacggt
     5221 gtacagtggt taacaaatcc tttttggata cgaatatcaa tgatcatcac gatgatttgg
     5281 cttagtgtag gatataacac tattttattt ttagctggtc ttcaagggat ttctaaagag
     5341 atatacgaaa gtgctgaaat agatggagca tcgaaatttc ggcaattctt tttattaacg
     5401 atccccatgc taaagccaat cattttaatg gccacagttt tagccactat caatggcttg
     5461 ggtaatttca atatcccaaa tatattcttt gggacttcca atggaccaga aaattcagct
     5521 ttggtcgtag gagtcaattt atataaaaca agttttgaaa tggttgattt tggaaaagcc
     5581 tctgctattg cctggacgat ggtgtttgta tctgttatat tatcggctat tcaatttaaa
     5641 ttcggaggtg ataaaaatga ctaaacaaaa gatacaaagc agaattttta cgtacgtcat
     5701 tattgtgttt ggtgccttaa tatttttgat gccatattta tatatgattg tagcttcaac
     5761 gcaaagtaat caagaaattt taagtggcgg aatcaatttc aagtttgggg atatgctatt
     5821 aaaaaactgg caagccatca tggcgagata taattatccc ttgattattt ggaattcgat
     5881 tgttattgct gtaatcagta cagttttagc tacagccagt tctacaatgg cgggttatgc
     5941 attagcaaag tatcgtttta tggggaataa actgttgttc aatacagtta tgctatctag
     6001 gatggtgcct ttttttacca cattgattcc tttattttac attatgagtc gtttaggatt
     6061 atcgaatact tatgcaggtg tgatcatccc ttcgattgcc agtacaactt cagtgtttat
     6121 gatgcgacaa tatagcagtc aatttcctac agcactgatg gaggctgcca gaatcgatgg
     6181 tgccaatgag tgggtgattt ttacgaaaat cgctgtaccg attttatcac ctacgattat
     6241 tactagtggt ttacttatct ttatgggaag ttggaatcaa tatctatttc cattagtcat
     6301 gctttcagat aaagataaat atacggttcc cttaattatc aaaaatatga cggtttctag
     6361 tacaggtgag ccaattaatt atggcgcaat tatgctaatt ttagcaactt cagtgatccc
     6421 aatggtcttg atttatgtgt gggcgcagtc taaatttaag gagaatgaca ttggagctgc
     6481 aaataagtaa aaaaaataac gaatgttaac catctagttc gttctaggag caactccaca
     6541 ttcataaaaa ttaaaagaat ggcgggatat ccatgaatga acagacgaag gaatggtcaa
     6601 gaaaaatttt gaatgtagct tctgaaaaga tagatgcgga attagacaga atggggaata
     6661 aaattccata tatttcttac aacggaaaat atacagacgt aataaaggac agaggaatcg
     6721 actggtggac caatggtttt tggagtggta ttttatggca gctctttcac aagacaaaac
     6781 gagaaaagtt caaagaggca gcaatacaac aagaacaatt attagaatct gccctgtacc
     6841 atttttcaga cgttcatcat gatgtaggtt ttttattctt accatcggct attgcacatt
     6901 atcaacagtc tcaaaacaaa catagtcgac aactagggtt acatgcagct acggtacttg
     6961 ctggacgcta taatatttca gggaactatt tagtagcatg gaatgaccat aaacctggtt
     7021 ggatgattat tgatagtatg atgaatttac cattactgta ttgggcttcg agggaaactg
     7081 gtgatccgag gttctctaaa attgctcggc agcatgcaga tacaacagct aagtatctag
     7141 tgaggaaaga tggatcaacc gggcatatcg cagaatttga tgcagaatca gggaaatata
     7201 ttcggttact tggcgggcaa ggatacaatg agaattcttg ttggagcaga ggaaatgcat
     7261 gggctttata tggattcgcg ttaagttatc gttacacaaa aaatccgtac tatctagcta
     7321 tagcagaaaa gatagcagta aattttattc agaaaatcga aaaaactggt tatattcctc
     7381 tcattgattt tgctgtaaaa gattcgaata ttgataccag tgctggagta tgcgctgcat
     7441 gcggtcttct ggaattagca ggatatgttt cctctgtacg atcaaaaatg tatcatgaag
     7501 ctgctatcaa tatactccaa aagacagaaa agcactatgc aaattggaat atggatgaag
     7561 atgggatcat tggaggcgga gcagaagcat accatagaga actgacttat aatgtgccac
     7621 tgatctattc tgattatttt tttattgaag gcttacttcg attagaacag tcggaattag
     7681 ctatttggta gtaaaaagag gagaaaatac aatgaaacat ccagtaccac tattaaggac
     7741 cagacaggat tactttgact tatttaaaaa gataaatcaa ccactccagc cgttttatcg
     7801 agcaagtggc gcacaaattg attatataca tcaaggggta ggatatggaa ataaaattgc
     7861 aggaatggaa ggattctctc gggttttatg gggagcgggg cccgcaattg accaattaga
     7921 tgaacgatgg ttgaaattaa ttctatcagg aataaaagct ggaacagatc cttcccatcc
     7981 cgattattgg ggagaaatac atgaaagaga ccagcgtatg gtagaaatgc ccgcaatgtt
     8041 attagcgtta tatcatcatg aacaattatt atggaaaaaa ttagatacag gagaaaaaag
     8101 ccagattatt cattggttct cgcaaatttt tgaatatgaa tgtgcagatg gtaattggca
     8161 attttttaag ataattgtcg gaacaattat ccaaaaattt ggttatccag ttaaagaatc
     8221 agcactgaaa gaagcatttg ataatataga aaattgttat cttcaaaatg gctggtatcg
     8281 agacagttca agaggtcgcc aagattatta taatcctttt gcatttcatt attatggttt
     8341 gatttatagc gtactagaac cagaaaagcc cattagccag atatataaga accgagctat
     8401 tgatttttct agagactata ttcatttttt tgcggaagat ggtgcaaatg tgccttttgg
     8461 tcgaagcatg atttatcgtt ttgcagtttc aagcttttgg tcagctatgt tgtgggccga
     8521 tcttttgcct tttaaattag gtgaaatcaa ggggttagtc aatcggaata taagatggtg
     8581 gatgcaaaaa gaaatatttg atgaaaaagg tatcctgaat attggttata cctaccctca
     8641 attgacattg actgaaccct ataatagtac gttatctcca ttatggctga ataagatttt
     8701 tttattgttg gcgctacctg aagaccatga gtattggacg gctaaagaaa tgcctatgcc
     8761 tattcgtgaa ggaactaaac ttctttcaga agccaatttg ctcatacaac atgataatgg
     8821 acatacattt tttttgaatg cgggacaatt agggccaaat ttccatacat tgaccaatga
     8881 aaaatattta aaatttgctt attctagtca atttggtttt tcgataccgc gagcgaatca
     8941 attgaaagca gaaatggcaa tggattcaat gctagggatt cagcgagcgg acacatatat
     9001 cacgaccagt tttaagaatg agagcatcaa aacctatgga cagttcatcg ttcgaaataa
     9061 tgtacgagac attgtatgta agaaagaggt acttgcctct acatgggaac ctactttaca
     9121 ggcaaaaatc agaacttggc ttattccttt tgaaggctgg cagattcgcg tacacaaagt
     9181 agaattagat acagaatatg ttctatatga gacagggttt gctattgaat gttctcctga
     9241 gaaagaagga ataatcatag aagaagacaa ggaaaactat agggtgagtg aagctggctt
     9301 ttcaggtatt atctgtttaa gtgacgatac tattaaacga aaatccacag ctattatggg
     9361 tttgcccaat acaaacttga tgacatggga aaacactttt attcctggat tagagggtac
     9421 atttggaaaa ggaacccatt ggctcggaac aggagtggtt gctcatcaag atcgggatta
     9481 tagtttagaa gtatggaaca atcgtcccaa aattgatttt gatgggacaa ccgggctaac
     9541 tatccaaaat aaaaacaata aaaaaagact aaaattatgc taaaggctgg tggggaaatg
     9601 cttgataaag tgatgtttga agagataata catccagaaa aatggataaa tgaatgttat
     9661 aaagaattaa tggaaaaaga atggaataat gagatcgagc gttgtcgcag acacacggag
     9721 aggttaatca aacgtatcag taacagtcag tatcaattcc caagcgagtg tcctataaaa
     9781 ggacgttatc cattaagaga caatattggt tggacagaag gattttggac aggaattaat
     9841 tggttgttat ttgagaagac gggtgatcct ttatttcaac aaacagctga aaaagcagat
     9901 atagatttta tccacaggat tgactgtcaa attatcacta atcatcacga tcttggtttt
     9961 ttgtattctt tatcaaccgt agcagattat aaaataacgg gaaatcgaaa aatgaaggaa
    10021 gcttcaattg atgctgcaga aacattactt cagcgttttt gggagaaggc aggagttata
    10081 caagcttggg gagatcctag tgatccttca caacaaggta gaatgatcat ggattgttgc
    10141 atgaatcttc cattattgta ttgggcgagt gaagaaactg gaaatcaaaa atataaaaaa
    10201 gctgctcagg ctcatattca aaatgcagca gattatttag taagagaaga tggttcaact
    10261 tatcatacat tttatatgga tgtcaaagat ggaaaaccaa gatatggaag tacgcatcaa
    10321 ggttatagtg ataattcctg ttgggctaga ggccaagcat gggggattta cggttttgca
    10381 ttaagttatc gttatttgaa agaacaaaaa tattttgatt taagcaaaat attagccaat
    10441 tattttatga atcggttgcc agaagatacc atttgttatt gggatttgat ctttgatgat
    10501 tctagcagag aagaaaaaga tacatctagt atggtgatca tggtttgtgg attattagaa
    10561 ttaatagaat caggtctatt agacaaaaca gaaaagaact tataccagtc tgctgtttgg
    10621 aaaattatgc gacaattgaa agaaagatat gctacaacgg aagaagaaga tggcttttta
    10681 acacagtctg tttataataa gcccaaaaat aatggggtaa atacaccttg tatttggggt
    10741 gattactatt atttagaagc tttagtgaga atgactagta attggtcttc ttattggtga
    10801 aaaaggtaaa tatggaattt ttgtttgata aaataaaaaa agaaaaacca aaacagcatg
    10861 aatggtttct gttttggttt tttcgttaat tttcatattc ttctaattcc aagcttagtt
    10921 cttcccattc cgtcaatttt gtttcttgat cttgtctagc agcttctaaa tcttggttca
    10981 gctcattgag ttttacgtga tcttccaggt tttctggcgc gaccatttct tgttccaact
    11041 gatcgatcca ttcatcgatc cgcgccattt cttcttcaac ctgagtaatt tttcgttgaa
    11101 ggctacggat aagcttttgc tgttctttgc tttggtaata atcagatttg ttcgtgggag
    11161 caggagcaga ttctggctca tcttgtgcca acaattcggc gatttcttct tcctgctttt
    11221 tctctaaata ataatcatag tctcctagat agactttgct gccattttca gagagttcga
    11281 cgacttttgt cgcgatccga ttgatgaaat atcggtcatg ggaaacaaaa agcagtgtgc
    11341 cgtcataatc gatcaatgca ttttctaata cttctttgtt gtcaatatct aaatggtttg
    11401 ttggttcatc caaaatcaaa aaattttctt tgttcatcgc taacttggct aaagcaaccc
    11461 gtgctttttc accgccactt agcagaggga ttggtttttc tacatcttcg ccagaaaata
    11521 agaaagcacc taaagcactt cggatctctt tttctggagt ggtaggatgc tcatcccaca
    11581 attctgctag gatcgtcttg tttgtatgca gatcagcttg accttgatcg taatagccga
    11641 tggtgacatt tgtccctagc gtttcttttc cacggataaa agggatctgg tgaatgattg
    11701 atttcagcaa cgtcgatttc ccgataccat tcggacctac tagagcaatc gcttcttgac
    11761 gacggatatc tagattgatt ggttcagata aggtgacatt atcatatccg atagcggcat
    11821 cttcgatttg caagactaca tttccagaag gcttttctat actgaataga aagtttgctt
    11881 gtttttcatc cccttgagga cgatccagcc ggtccatttt ttctaatgtc tttctgcggc
    11941 tttgcgctcg ttttgtggta gaggcgcgaa caaggctccg agcaacgaag tcttctaatt
    12001 tatggatttc tgtttgctgc ttttcataag ccttccattc acttgctaac tgttctgctt
    12061 tcaaatctag atatttcgaa tagttacctt tgtaatggcg catttttctt ctgcttaatt
    12121 catagacttc attgactact ttatctaaaa aataacggtc atgggaaaca atcaataatg
    12181 caccagtata gctttgcaga taattctcaa gccaggaaag tgtctcgata tctaaatggt
    12241 tcgttggttc atctagaatc aaaatgtcag ggtgttgcaa aagcatgcga gctaaagcta
    12301 aacgtgtgcg ctgtccgcct gataaagtat cgattttctg atcataaaat gaagaatcga
    12361 atttaaaccc atgcaatacg gaacgaattt cattttcata accatagccg tttcgatctg
    12421 aaaaatcgtg ttgcagccgg tcgtattctt ttaaataaga actataagca ggtgtgtctg
    12481 gcgtaccttc gctaatgaaa atctctagct cgcgcatccg ctcttccatt tgtctaactt
    12541 gttcaaaagc tttcagcatc tcgttccaaa ctgtttcatt ggaatcaagt cctgtgtcct
    12601 gagcgaggta tccgagagtc gctgttttat ttttagcgat ctttccttca tcaggtgctt
    12661 caattccagc aatgatcttt aataaagtcg attttccagc gccatttctg ccaactaaag
    12721 cgacacgagc gcctgtactg atttctaaat gtatgttttc aaataagact tctgcaccaa
    12781 aatggcgagc aatctgattg gcttgtaata atatcatgga atcctctttt ctactttgtg
    12841 aattctcctc aaataaaaaa ctatttttca attagtagtc taccataaat tccattatac
    12901 tgcgagattt gcagaaatga taaataaatg aataaagaat aaaatttcac aatatctgag
    12961 caatggaatt gattttttcc tataaatact gctatgatgg agaaatcaaa gatgtaatta
    13021 gacaatacgt ggaggtcatt tcgtgaaaga tcatattatt ccaaaggcga cagctaaaag
    13081 actgcctttg tattatcgct atttaaggat tttaaataat gctggaaaga aaaaagtgtc
    13141 ttcaactgaa ttaagtgaag ccgttcaagt agatagtgca acgatccgtc gagatttttc
    13201 ttattttggc gaattaggaa aacgtgggta tggttatgat gtagaagatc ttatgcactt
    13261 ttttgcaaag acattaaacg acgatcaatt gacaaatgtt gcgctgatcg gtgtagggaa
    13321 cttgggaagt gctttactaa aatacaaatt ccatcaaagt aatagcatac gtatcagttg
    13381 tgcttttgat gtgaatgagg aaatcgttgg gcgcatcgta gacggtatcc ccgtttatcc
    13441 aatggaagat atgtcagaac aaatcaagat ccaacaaatc gaagtagcaa ttttaacgat
    13501 acccgctgaa agagcacaag aagtagtcaa tcagttaacg gaagcaggaa tcaaaggaat
    13561 cttgaacttt accgctgctc gattgaatgc tccgtcagat gtattgatcc aaaatgttga
    13621 tttgacgaat gaattgcaaa ccttgatcta tttcttgcat tctgacgcaa atgcgcattc
    13681 cgaaaataca gcagaataaa aataaaaagc gattgaacaa atgaaggcaa aactttcact
    13741 tgttcaatcg cttttttctg tttggacaac gattattttg ttcggaagat ttcttcataa
    13801 gaaagggaat catcttctgc tgtcagttca tctagtagat aacggatttt tttaaagtcg
    13861 atgaaccgtg cttgccgaaa gacttctttt ccttgatgaa aaatcaggac aactggcgca
    13921 gtcagcactt caaatttact agctacttct ggtgtttcaa atgcatccaa atgaagtgcc
    13981 ggaatatcat aatgtgtgag aagttcctct agtcgtggac gaacagcgtg acaaacactg
    14041 caattaggca tcgaaagata aatgacggca acggatgact gttgcagtat gtcttgaact
    14101 tcagtcaagt tagtggtttg tttcattttt attctccctt ttatgataga agtgatatgt
    14161 atgagacctt aatttaatgg gaataacaga aaaagtcaat tgaagtgctt tggtaaaaaa
    14221 taagagttgt gtaaaaattt acagagcaac ttgttttttt tgaataaaag cgtatatttg
    14281 caaagcgaat gaactagaaa taaaaaggag gaaactcatg ttttctttta ttccaaattc
    14341 ggttttgcag atgagtacga ttttcttatc gatcgttatt gaagcattac ctttcgtatt
    14401 attaggctgt cttatctcag gcgccttaca agtatttttg accccagaac gtgtgaagag
    14461 atggcttcct gaaaatcgta tcttctctat ttttactggt tcgatcttag gatttttctt
    14521 tccttcatgt gaatgcggca tcgtaccgat cgttcatcaa tttgttaaaa aaggcgtacc
    14581 agttcacaca gcgtttgctt ttatgctgac tgctccgatc atcaatccaa tcgtgatttt
    14641 ttccactttt atcgcatttg gcaattcttg gcagatggca agctggcggg tgataggcag
    14701 cttttttgtt gcccttgtga ttggtgtctg gcttgcctat tttcaaaaag acgctgtatt
    14761 gaaagaaaaa atcacgtcta aagtagcaga aaatcaccat catcatgtcc atgaagaaga
    14821 acaaagtgag aagaatgaac caaaaaatca gctgatccat caaactgggc atgtgttgac
    14881 acattcaatc gacgaatttt ttgatacagg tcgttatttg atcattggcg gattaatcgc
    14941 agctgcgatg cagacttatt taccaactag aattatgcta acgctcggct caacaaagat
    15001 tttagccatt atcatcatgc tgttattggc ctttacgatg tctctttgct cagaagcaga
    15061 tgcgtttata ggatcttctt tgttaagtct gttcggcaca gcacctgtag ctgccttttt
    15121 ggtctttggc ccaatggtcg atatcaaaaa tctactgatg atgaaacgat attttacagg
    15181 acgctttatt ttatcgctga tcggactgat tgcccttagc gttattggat tcacattatt
    15241 tatttaggag ggaaaatatg ctgcggtttt tgattttatc tgggtatttc gaattgatga
    15301 tgtacttgca agtttctggc aaactcgatc aatatatcaa tgtccattat cgctacttag
    15361 ctattctttc aatgatttta tctgctcttt tggctttagt tcagctgtat ttgtgggtca
    15421 aaaacggaaa agcagaagaa aaggcacacg agcacgatca tgaccatggg ttgtcaaaac
    15481 cttatcaacg tggaatcgct tatgtactgc tagctttgcc agtaatcgtg gggacgttgt
    15541 ttccaacagt tagtctcgac actacgatcg tagaagccaa aggatttaat ttcccagtaa
    15601 gtaaagaatc agtaggggat ccggagatgc agacacaata cttaaagccg gataccagta
    15661 tttatttcaa caaatcagac tacttagacc aaatgaatga gttgaaagaa aagtatgggg
    15721 ataagcaaac tatcaagatc acagatgaaa actatttaga agttatggag ctaatttata
    15781 attaccctag cgaattcatt ggcaaaaaaa tcagctatga aggatttgtt taccaaacac
    15841 cgaacgatag caatacagat atcttcttgt tccgattcgg cattatccat tgtgtggcag
    15901 attcaggagt ctttggtttg ttgacgcata tgccggaagg agtaacggtt aaaaatgatg
    15961 aatggtataa aatagaagga accatccaat cagactatta tcaaccattc aagcgtgaaa
    16021 tcccatctgt agttgttaca aacgctgaga aagtctctgc tccgaaaaat cagtatgtgt
    16081 atcgttcttt ttaaaagaac aaaataaata aacatagctc agacagctga cacgcaagga
    16141 cgtcggttgt ctggcttttt ttgcatgatg tattcatttg tttgatattt attttagaag
    16201 cagcatcctg aaagtatttg atatcggttt caaaggtgtt ggaaaagatt gtgaagagaa
    16261 ttacaattta ttcctaacat gtatgttgaa aagttgtatg aaatgatttt gatagtaaaa
    16321 tatcgagtgg aggtactttt tatggtaacg cgtatattta ttttggatga tagtttggtt
    16381 tttgaaaaaa tgctagtaga aattttggaa gaatccagaa ttttattaag tccttggaaa
    16441 tttgagatcg ttcagggatt gaacgttata caatttgttg aattcattaa aaataatgaa
    16501 atctattcct cagatatttt tttcctagac atcgatttga acgcctatta tagcggtatc
    16561 gaactaggcg aaatgcttag agaaaagaat gaaaagtgta aaattgttta tctaacgtct
    16621 catccgaata aagcaataac tgttattaat cgaaagatta cgcctagtgg ttatttgata
    16681 aaagaattca cttatgaaaa aaataaaaaa agcatccaaa gttacttatt gaaagaaaaa
    16741 gaaaactatc ctgttcaaca tgctacgaaa aaaaggtgga caactgtccg ttatggtaat
    16801 agcgatcact atatccatta cgatgaaatc ctttatctaa tgagcatgcc aggttataaa
    16861 aataaaatag gcctagtatt gaaagatgaa gagattctcg taaacggcac aattaaaaaa
    16921 tataaaaaat cactcgaata tgagtattta tgtaaagatt taaaagctta tatcattaat
    16981 actgaaaaca ttacatctat tagcagagta aacgaaatag taacatttgc aaatggagaa
    17041 caactagaga tgggaaagag aagtattgat aaactaaaga attttatgca agaaaagata
    17101 gaaaatgaat cattttaaag aaaacaaaga ctcatatctt acctgttaga agagtgtttt
    17161 cctgtagaag agaacaaccc aagtagcagc ctagtcgttt ctatcctttt ttgttatgca
    17221 ctctttttga catttactcc accaataacg aagggtacaa taaaaagtaa taacaaacaa
    17281 aaggagtaca gaaatgattg aattcatcaa tgtaagtaaa agttacggga aaaaacaagc
    17341 attaaaagac ttgtctctct cgataagaca aggggaaatt tttggatttt taggacataa
    17401 tggtgccgga aaatcaacta cgattaaaag tttagttagt attattgaac catcatcggg
    17461 aacgatttta gtagatggaa tgaaattgac ggaaaatcga ttgagtatca aacaaaaaat
    17521 cggttatgtg cctgattccc cagatatttt tttacaattg acggctggag aatattggga
    17581 cttgatttca gctgcctatg aattagacac acaaaaaaaa gaaaagcgtt tagcagaatt
    17641 gacagcgctt tttgatatgt atagtcatca aaatgaaaca atcgcaagtt tttcacatgg
    17701 aatgcgtcaa aaaacgattt tgatcggcac gttgctgcca gatccagata tttgggtatt
    17761 ggatgaacct ttgcaaggac ttgatcctca agcagcattt gatttgaaag aaatgatgaa
    17821 ggctcatgcc gcaaaaggaa aaacagtcat tttttctaca catgtattgg ataccgcaca
    17881 acagttatgc gataaactgg ctattttgaa gaaaggtgaa ttgatttatc agggggcagt
    17941 tagtgaatta ttgaatggct cacctgatga aacgctagaa aagatttatt tgaaaatggc
    18001 tggaaggcaa gcgacaggag atggaccgta tgtataagcg acaattatta gaattaattc
    18061 gagttaatct tttatatgtt aatccacagc tcacgaataa agcaagagag aaaggaaaaa
    18121 agggaaagcg attaactatc tatcttttgt ctcaaaatct tttttcaggg ctgatttttt
    18181 tactgattta cggatttgct atgtttgcta tgaactttgt taaattgcca ggtttcttta
    18241 cttattacgt ggcacttttt ggtatgttag gtttaactca aagtatatca gtcatttata
    18301 atatcttttt tgaaggaaat gacctgcaag tatttttgcc tctgcctttt ggacaagaac
    18361 aggttttttc agcaaagatt ttggtagttg ctttgaccat cattcctttt gtttttccgc
    18421 ttctagtcat gtttatttta accggatggc gagctgatct agggattttg cttactatca
    18481 ttatctcaat ccttttgttt tctctatttt tgattattct tttttgtctg gctagtttga
    18541 ttgtttttgg tttggctcga acagcagtgt ttaaaaaaca tagaaaaaca gtttctagta
    18601 tcttgcttgg cagctcgatg ctgatcgcat ttgctggaat catgtggatg aatcatcaaa
    18661 cggggactat tgaaaatgag ttaacagatc ggcatccgat ttcgatatta ttgccattct
    18721 atgatgctgc cagtcagcca ctttctcaac aaggtttatt gggttgggga atactagcta
    18781 tcacggctct gggatttttc cttgctgtaa aacagctgat agtaccaaag cttacagagc
    18841 aactgactga ttcaaacagt gagttcaaaa taaaaagaac ccataaaaag aaccaaaatg
    18901 tgcatcagct tttatttagt tacaatgctc aattagtaaa agaacctaat ttgattatgc
    18961 aagttctttc ttcttccttg ttaactccaa tcatttttat tcttgctttt gccctaggag
    19021 gcgaaataaa attaactgat ttagataata agttaatcgg tgttgttttc ctggtaggga
    19081 tcgcattggc tttcttaaca gttaatcaga cttcttttat cagcaatctt atttctttgg
    19141 atcaagaaaa ttttctgttt atccgttctt tgccaatctc tatgaaccaa tatttaaaag
    19201 aaaagttcag attcggatgg atattgcaga gttttttaac tggtgggatc gcattattaa
    19261 gcggactgtc ttttcagctg cctttgtttt ttatccctag cttgctattt ggttctttat
    19321 tcggctgcta cttattgtca ctacgttatt tcgctagaga ttatcggttg ctgctgctga
    19381 actggaccaa tattaaccaa ttatttaaca gaggttcagg aagtttgggt ttagtcgcaa
    19441 ctatgatggg ttcattgatc atcagtatta ttcttatcgt cttatacgga atgttggctt
    19501 tgttttatcc attttggtta gtgaatattc ctgtgatcgg tttagttcta atactttccg
    19561 ctctatggat cgtttattat caacgaacgt tttggaaaaa atttgaataa agaagagggg
    19621 ccgtgacaaa aatcatgtcg cagccccttt tttacagata gagggtgttc aaaagtcagt
    19681 ttctcagtat taagtcaatt ttatgtaaaa atgtgagaag ctatttttac aaaacttttt
    19741 ttaatactca taattacttc ttattgctcc tgcggttact tgtcgcaatg tttgtgagat
    19801 attttgtcat cgctcaaggc tgaacgtctc tttcacaacc ttttctttgt tacacttcgt
    19861 ttatttaaaa agccgttttt ttcgtttcat catgattgcg tcacgcttta ctttgaaatt
    19921 acggatgtta tggttaaaga cttctgataa tacaagacct aaagcgattg ctccagcaat
    19981 cataattgct tggaccgcat aatgagccgc ttctcgatag gattcagtaa cgagatttcg
    20041 aaccgcttga taagctaaac caccagggac tagaggaacc atcccgggaa tattgaaaat
    20101 cgttacgggc attttcaaac gtttagagaa aagatcgctg atgaatgcta ctcctaaaga
    20161 accaagcaat gaaccaaaag cagcagatcc gcctatttga acacagatcc agtagatcat
    20221 ccagccaaaa gcaccagaaa gaccacaaca aatcaatgaa cgcctaggta catttgtaat
    20281 aacggcaaat gccgcagtcg ctaaaaaact aaaagaaaac tgaataaata tcgtccccat
    20341 acttgtgttc ctttcctaat aaaaaagctg gaaaataaaa gcaatcgcaa aaccgatcat
    20401 cgtagcagtc atcatcgctt ctgttccgcg agaaactcct gagacataat gtccagctag
    20461 caaatcacgg atagcgttcg taatttgaac tccaggaact aaaggcatca cacatccgat
    20521 aatgattaga tcctgattgc ttcctaagcc aaaacgtacg ctgtagatag cggctatgcc
    20581 aatcaataaa gaagataaaa attccgataa aaatttgatt ctaaaaaatt tcaaactata
    20641 tagataagct gcgtatccca atcctccaat gactaaagtc acaggtaaat cggtcatctt
    20701 tccgccaaat aacaccataa ttgttccact gatcactgcc gcgctaacga tttgcaacca
    20761 gacaggaaag aaagaacggt ctcggtcgat tgcttgcagt tcgcagtaaa gttcttctaa
    20821 tgttagctct gaagcaacat atttacgtga aagatcattt acttttacta ctttttctaa
    20881 attaatcgaa cggttggtaa tttgttccat tcggatggtc gaggtacgat caagaccaat
    20941 aaagagaccg gtttgagtga catagctgac caaacggaaa tttccagaag ctaaagcaat
    21001 tcgactcatc gtatcttcta cccgatacat ctccgcattg ctttccatca tgattttccc
    21061 agcgagtaaa caagtgtcca gcaacagatc taattctttt cccgtcatag catcttctcc
    21121 aataattgat tgattagttt tattttacta caaaacatac cagaagcgta aagaataaaa
    21181 aaacttgagg aaggaataaa agaggcgtat actgtttttg tattgcgggt gtagtttagt
    21241 ggtaaaatcc cagcttccca agctgatgtc gcgggttcga ttcccgtcac ccgcttaaaa
    21301 taggttgtga aaaagctgct ctgcaacaag taataagaac agccaagcaa aaacaacttc
    21361 ttatgttttt aacttggatg gacttattac cgcagatgca agcttttgcg caccgtttac
    21421 caagcggttg tgaaatcagt gtattgtctt gagtatgaag aaaatttttg aataataata
    21481 aataccttac agatagagtg gtgactgatt agagacgagg aatccgtctc ttttttgtat
    21541 ttttaagcca tcttaaaaca ggttttattt tttgctgttt ctatttcttt ctagtattta
    21601 tttatagggg aacatatgtt aagatttagg gagctgttag taaaggagta aagaaatgag
    21661 caaaaaaaca accaaggcga tcgtggggat cgtcgctgct gtgattgcag gggcattcgg
    21721 tgtttccacc acacaaaaag attcagatat ctttcagcag attacaacta tttttgattc
    21781 cacaccacaa gcttctcaag tagcggatgt gccagaatat gatggaaaac atcaagaaat
    21841 agaaatcaat gggaatcagc cggaatttac aaaagaggat cttagccttt ccaaaggaac
    21901 atgggaaagc tattcaaata ttgataaatt gaacagagta ggacaagcca atgctatgct
    21961 tggaaaagat ttgttcccta ctgaaaagag agagcctttg tatattgacc cgacaggttg
    22021 gaaacaaaag aaattaagtg acgggcaatg gttatataat cgcagccatt taattggttt
    22081 ccagctgact ggtcaaaata acaacattaa aaacttgatg acagggacac gttcgttgaa
    22141 tgctccatac atgcttgctc acgaaaatga tattgcggca tatatcaaag aaacaaatca
    22201 tcatgtacgc taccgtgtca ctcctcattt tgaggggaat gaactggtag ccaggggagt
    22261 gcaattggaa gcagaaagca ttgaagacaa aaaaattgaa ttcaatgtct ttatctacaa
    22321 tgttcaagat ggctacacga tcaattatga gacaggacaa gcaacaaaaa caaaaaatta
    22381 gtagcagcta ttcgaatttt ttatgaatat tatgaaaaaa ttgtgtaaaa acatacattc
    22441 aaagaaatat agcgtatact ttatgaaaag agtacttttg ataaagctac agaaaaaagg
    22501 aaaaaaacat acaaatagtg tttcttaatt tattcggaag tttggaatat ttgtttgtag
    22561 ttttcccttt cttcgagtaa aataaggttg tagaaggtaa gctggaatta ccttgcggga
    22621 aagaggtgta gaatcatggg ttttacagat gaaaccgtac gttttggttt tgacgatagt
    22681 cgcaaaaaag aagtgagcga aacattagcg attgtttatc gggctttgga agaaaaaggc
    22741 tacaatccaa tcaatcagat tgtcggctac ttgctttctg gagacccggc ctacattcct
    22801 cgttatcgtg atgctcgaaa tttgattcgc cgtcacgaac gcgatgagat catggaagta
    22861 attgtcaagg actacctctc aaatcatgga gtgaatttat gagagtaatg ggcttggatg
    22921 tcggttctaa gacagtaggt gttgctatca gtgacccctt tggctggaca gctcaaggag
    22981 tagagatcat tcggatcaat gaagcagaag aagagtttgg ctttgatcgg ttgaaggaat
    23041 tagtagcaca atatgaagta gaccggtttg tagttggatt acctaaaaat atgaacaatt
    23101 ccatcggacc aagggcagaa gcttccatgt catacggaga aaagatcaaa gaactattct
    23161 ccttgccagt tgattatcaa gatgaacgat taaccactgt tcaggcagaa cgtatgctag
    23221 tagagcaggc aaatacttca agagccaaac gaaaaaaagt catcgataaa ttagcagctg
    23281 tgatgatctt acaaaattat ttagacggct caggtaagca attattttaa taataaaaga
    23341 aaagaggtaa tcttatgaca gaacataacc attcccacga ccatgaagga cacgaacaca
    23401 tcacattggt ggatgaccaa ggaaatgaaa cattatatga aatcctacta acagttgacg
    23461 gacaagaaga atttggacgt aactatgttc ttttatatcc tgctggtgtt tcagaagatg
    23521 aagatgtaga attacaagct tatgcataca tcgaaaacga agatggaaca gaaggcgagt
    23581 tagaacaaat cgaaacagac gcagaatggg acatgattga agaagtattc aatacattca
    23641 tggcggaaga agaagaatag aacaaaagaa tgaattaata agatataaaa aaagggattg
    23701 tgacaaacgt cttgtcacaa tccctttttc caaataaacg atgttcaatc agctgcccct
    23761 cggcattcgc tcgaaaaacg gcgaaatatg aagaactatt ttgccatttg tttctctaat
    23821 aatcaggtca aaacgttgac ttcacagtct cttccttaaa cggtgttctc ctgccaactc
    23881 ctcggtcaca agtcacaata ttcaacaaac atgagaagct gttttttgaa attgttcctt
    23941 gtgactcgga gctgaacggc aggctcacga cctctttctt aaacggtgtt caaaagcttg
    24001 catctgcggt aataagttca accaaacgaa aattatgagg agctattttt gtttggctgt
    24061 tcttattact tgatgcagga cagctttttc acaacctctt ttcgtttagc actaaatttc
    24121 cttaaaaagt agaaaagctt tcgaagcctc gtttgtttct tttactgtat aataaaagaa
    24181 aagagtaaca atcgctaaaa gatataaaca agagaaagga aatacaagat gattggtctg
    24241 atttattttg tggtgatcgt tctggcgaac acagttggcg ctatttccgg gatgggtgga
    24301 ggcgtgttga tcaaaccgat tcttgatctt atcggcgccc attctgttgc agggatttct
    24361 ttttactcaa cagttgcggt attcacgatg tccattgttt cgactgtgcg acaagtatca
    24421 agcgggaaat cactgaattg gcagattgtc ggttgggtat ctagtggagc tgttgttgga
    24481 ggagtagtcg gcaacattgt attcgaagtt tttttacagt tatttgaaaa cgagaagcat
    24541 gtgcagctga tccaaatttt tttgacggta ttgaccttgg tttttgcgtt cttctacacc
    24601 aagcatcatc agccaaaatt tcatttgacg tcatggactt ggtaccttat atgtgggggg
    24661 gttctgggct ttttagctag ttttttaggt attggtggtg gaccagtcaa tgtttctttg
    24721 ttgatgctga tgtttgcatt gccaataaaa gaagcaacac tatattctct atctacgatt
    24781 ttcttttctc aactggcaaa attggtaacg attgctttaa caagtagctt tatgcgcttt
    24841 gatttatcta tgttgtttta tgttatcccc gctgctgtta ttggaggttt gtggggagca
    24901 agatttagca gggttttgtc tccaaaaaaa gtgaccttta tttttcaagt aattgtcctc
    24961 gtggttcttt tggtcaattg ttacaatgcc tttgtcatct tacaaacatt aggagaatct
    25021 agactaacgc aagtcaaaat cactgaaaca tattgttcta aatgaaaaaa agagtatact
    25081 atgataaaaa aattcatttt aaaaaatcag aaattggctg gtgatttgga tgttacggga
    25141 aatgcgttta gaagatgcag aagcactacg tgaaatcaat gcggatcaat tagggtatga
    25201 tataccatta accgttacgg tgagacaaat gaagaaactg ctgaaggagc cagagaaaaa
    25261 ttttttcttg gtttatgaag atcctacttc taaaaaagca gctggctatg tacaagcgga
    25321 gtcctatgaa tcgattttta gcgatccaat gttcaatatt atggcacttg ctgtgtctaa
    25381 atatgctgaa aaaagaggca tcggaaaggc tttgatgact ggcgtagaag aagaagcgaa
    25441 aaaacgagga ataacgacaa taaggcttaa ttctgcagaa tacagagtag aagcacacca
    25501 attttatgaa cacatcggtt atcattccga taaaatgcaa aaaagatttt tgaaaatcct
    25561 gtaaatcttt atcaaaaaaa tacccgatca ccaagccgat acttgatgat caggtatttt
    25621 tgtttttttg actaagaaag taattgcatc aagaaaccag agatgatcaa aacgatggca
    25681 ccaccaagtc gattgcccat ttgagcaaaa gcaataagtt ccatccgatt agctgcagat
    25741 aagacggcta catttcctgt tccgcccatc gagttgttgc ataagccagc agtaatagtt
    25801 gattcaattg gatataaacc aaacaaacga ccgacaaatc ctgaaacaac tgaaatcgcg
    25861 ataacacttg tcagacataa gacaacaaat tgccatgtca aagcagaagc taatacattc
    25921 agattcagca acgcaatacc gataccggca agaacagctg ctgtcaagtt tttgacaatc
    25981 aagttattga acatgatagc tgcatcttca taatactctg gtaataggtt ggcgatttta
    26041 cagatgacaa cgataataat cataaatgcg taagcatgga ctttaggaac aaaatagtta
    26101 ccaatttgtc ctaagatgaa gaaagtcaat gataccatca aaccaacacc taactgtgga
    26161 atggaaggtt tcacagttcg aggttttcca tcgccaagat cgcctttttc cattaattta
    26221 ccattgccgt tgaattttgg cattgattgt ccagcacgag caactaatgc tgcaccgatg
    26281 atagctaaga catttgtcat tgctgtcgca ggaatcaggc gggaaatgat agaagaagca
    26341 tccgtaccta aaacatttgc ataaatcgtt gataaaggaa cggctccggc tccaacacca
    26401 ccagccatcg caggtaatga gatgtataaa acggaatttg caaaaccatt tccgataagc
    26461 atgcccacga gtcctacggc aaaaaaggag acgaccattg aaataagtgc aacgggaata
    26521 aaacgaacag aagctttcat caatagatta cggttcatac caaggatgct accagtaatc
    26581 aaggcagcaa tataaaagtc ccaaaagccc atattgctga caaagttttc tgtggatttc
    26641 actacatatt caggtaagat cccaaatgta gcaatcgcag cagaagcgaa gatcgcaaaa
    26701 acggaaccac cacccaaata acttttgacg atagggattt ttgtcccgat aaaatgaaat
    26761 aaattaccga aaataaccaa aactgcgatt ggtccaatca tattcgtcgg taatttccct
    26821 aaagccattg caatgatcag gacaaggacc atgatcaaat aaagtggcaa acttacgccg
    26881 gtgatttttg tggaccaaaa accttctttt ttcttttcct caactgcagt tgcagttgtt
    26941 gttttttgtg ccatttttac agatcctttc ttattctgta tcatatgaaa aaagccaaag
    27001 aaaagcgaca aaagagcagg agttggctgg ccgctgtttt gccgcagtcg cttttttctt
    27061 tttagacgtc atacaaagtt tctgtatgtt cttttactat aacgatctta atcttctaaa
    27121 gatttatatt caggaaccca tttagcagat tcaactgctt ctttgatatc cgtgatttct
    27181 tcacgattca agttttgtgc gacaactgat tttgcgacag tttcagcaat cgtttgagaa
    27241 aattcagtga tttttgctac tggaggcaag atagctgctc ctggttttgt tacatcaacg
    27301 attccgccta atgctcgact agcttgagaa atgatttcag catttactcg tgtagctgtt
    27361 gaagcaatca aaccaagtcc taaacctggg tacatcaaag cattatttgc ttgaccgatt
    27421 tggtaagtta caccattata ttcaacatca gcagcaggga tacctgttcc aactaatgct
    27481 tttccgtccg tccattctat taaatctttt gcttttgctt ctgccaattt tgttggatta
    27541 gacaaaggca tgatgatcgg acgtggtgta tgagcagcca tttctttcac gatttcttca
    27601 gtgaatgctc caggttgggt agaagtacca atcatgattg tcggatggat cgcttttaca
    27661 actgcttcta agtttgtcaa ttcatcagca ttagtgaaat ctgagcgttt gcgagcgaat
    27721 gggatttgac caggagtcaa tccttctgta tcttcaaaca ataaaccttg tttatctact
    27781 tgatagaaat gttggcgtgc ttcttcttca ggtacacctt gacggatcat ctcatctaaa
    27841 aggatgtttg cgatacccac accagcagta ccagcaccaa atgtcaaaat tatttgatct
    27901 ttcaattgtt caccggatat attcaatccg cctaatacac ctgctaggac aacaattcct
    27961 gtaccttgga tatcgtcgtt gaaagtagtg atttgatctt cgtatttttc taagatcgct
    28021 gcagcatttc ctcgtccaaa atcttcccaa tgcaataaca attctgggaa taattcggta
    28081 gctgattgaa cgaattgatc gatgaattca tagtaagttt cgccttctac acgagcatga
    28141 cgattgccta agtataaggg attattcaat aattcttcat tattagtacc agcgtcgatc
    28201 gatacaggca aaacttgtgc tggattaata ccagcagcag cagtatagac catcaatttg
    28261 ccgatagcga tgtcaacacc gttcacaccc cagtcgccca tacctaagat tccttctgca
    28321 tcagtcacaa caatcaaacg aatgtctcgt ccatctgccg cgttcttcaa acttgctttg
    28381 atgttttctg gttcgtcgat tgaaagaaat gcagcatctt gcggtttcaa gaaaatttcg
    28441 ttgtattgtt cgattgaatc cgcaacgaca ggatcataaa cgacaggcat gaattcaact
    28501 aaatgttcgc ccatcaattt ataaaacaat gtccgattcg tattgaatag attcattaag
    28561 aagatacgtt tttccaaatc tgtttgtttg cttaagtatt gaccataagc ttgaacagat
    28621 tgttgttcca gtgtttggac agtacttgga agcataccag tgattccata ttttgcccgt
    28681 tcttcttttg taaaagctgt tcctttattt agaaaaggat tatttaataa gtttaaccct
    28741 gtaagcatga atgattcctc ctaagtttta atacaaacga agtatagaag tcagatgatg
    28801 atatagtcaa aaagagcagg tatgataaat tttatttata tgtaatatag ccaaaagaaa
    28861 tcaaaatagc agtatttgtt ctttttatat cgacaatcct tgcttgaata ctatgcgtat
    28921 ctcttatatg ctagagaaag aaaagtagaa gaataaggga tagatggaat ggataaatag
    28981 gatttctgag gaaaaaagca catgatagcg ttgataggag tcacctatgt caggtgataa
    29041 aaatacataa gtagaaaatt aagaggagga agagaaaatg aaagaagttc aattgattag
    29101 aaaaagcgaa ctatcagaag gcggttgcaa tgcatgtggc gtagtagaag cgacaagcta
    29161 tacattaaaa ttagattcca atcaagcaat aatctctgaa ctgacagtag gcggtcttgt
    29221 ggacagtctg gcgcttgctg aaggttttac tggagaagat atttatgaga tgttttcaga
    29281 agtccgtcag ttaaaaaaag gagaaaaatg catagaggtt catcatgaga gcccgaatgt
    29341 gaggttcaaa cgtggtgata atgagatgat cttcaagaat catgtgtctg atcacactga
    29401 actatatgaa atcgtgaatc aaatactgtc tgggattttt gaattaggac cttatgagtt
    29461 caaggaagaa aacggtaatc caaaattaaa tgaagaatgg caagaaacga ttgaaacaca
    29521 aagaaacaat ccacacttat ttcaatagtc ttccacagta tttcgtaaaa atgttgatga
    29581 aaaagaaaca ccgtgaatca gaggtcgtga aaaacctgcc ctgcatcaag taataagaac
    29641 agccaaacaa aaatagctcc tcctattttt cgtttggttg ggcttattac cgcagatgca
    29701 agtttttgaa caccgtttaa ggaagagact gtgaagtcaa cgttctgacc tgagtattag
    29761 agagacaaat ggcaaaatag ttcctcatat tttgccattt tttgagctaa taccgagggt
    29821 cagctgattg aacaccgttt tatcgagagg ttgtgaaaaa gtcgcttagt ttcgagtagt
    29881 aagaaaaatt ttgtgaaaat ggctttccac atttttgcat aaatttgact taataccgag
    29941 aaaatggctt ttgaacaccg tttattcaaa aaaagggact ttgacactac ttttgtcaaa
    30001 gtcccatcgg ttttttatct atttttatta caatcatttc ttccagagta gtgacatctg
    30061 gaatggctga aatgtaaaca gattatttgc ctgtatcaat gtcgaagagt aatataaaaa
    30121 gtgtaaacgc tatcatattg ttcatcattc aaattttata gagagaaggc attgagatgg
    30181 aaaaaacata tcaagcaggt acaaatgaag gaatagtaga ttttatcaat atggaagatt
    30241 tagaaatcgc ggcttcacaa gtgattccag ctggtggtta tggttacatc agcagtggtg
    30301 ctggcgactt atttacttat caagaaaatg aacgggcatt caatcatcgt ttgattattc
    30361 ctcatgtatt aagagatgta gaattacccg atacaactac acattttgac gaagaaatgc
    30421 tgactgcacc aatcatcatg gcaccagttg ctgctcatgg attagctcat gtgaaggcgg
    30481 agaaagcttc tgcaaaaggt gtagcagatt ttgggacgat ctatacagca agttcttacg
    30541 cttcttgtac tttagaagag ataagagaag caggcgggga gaaagcaccg caatggtttc
    30601 aattttatat gagcaaagat aatggaatca atttggatat tcttgaagtg gccaagcgta
    30661 atggagcaaa agcaatcgta ttgacagcgg atgcaacagt tggcggaaac cgtgaaacag
    30721 accgcagaaa cgggtttacc ttcccgctgc caatgccgat cgttcaggct tatcaatcag
    30781 gagtaggcca aacgatggat gctgtatata aatcttccaa acaaaaactt agtccaaaag
    30841 atgtagaatt tatcgctgct cattctgatt tgccagtcta cgtgaaaggt gttcaatcag
    30901 aagaagatgt ctatcgatca ttagaatctg gtgctggtgg aatttgggta tctaaccatg
    30961 gtggccgtca attagatggg gggcctgctg cgtttgattc gttgcaatat gtggcagaag
    31021 cagtagataa acgtgtacca atcgtgtttg acagcggtgt acgacgtgga caacatgttt
    31081 tcaaagcaat cgcatcaggt gccgatcttg ttgctattgg cagacccgtt atttatggat
    31141 tgtctttagg aggaagtaca ggagtccatc aagtgtttga tttctttaaa acagaattgg
    31201 aaatggtcat gcaattagct ggaacacaaa cagtagaaga tatcaaaaaa ataaaattgc
    31261 gtgagaatcg ttttatttaa gaaataaatg acgaaaaaaa taggagtgcg gcagatgatg
    31321 ccgtactcct atttttatag ttcaaggatg tcactaatag aataatttga aacgaaaaaa
    31381 taaaattgaa atattttttc atacttaata aaaaaggaag aatattcatg taaacgattt
    31441 tattgttttt ttctctttta tacaaaaagg gtcataattt caagtaagcg gatgaattca
    31501 tgaggatttt ttgcatactg tctctactat acttaggtca ctaagaaaaa ggagtgattt
    31561 attatgaaaa agaacatgat tgggacatta ttactttcaa cacttcttct aggcggcttg
    31621 gcaacaccag cttttgctga gggtcaagcg acttcaaaag gagatattac ctttactgaa
    31681 ccaacaaata ccgttgagcc tcttaaccct actgatcctt caaaaccagt agagccagca
    31741 gatccggaaa atccagcaac tggccagaca ggttctttaa cattggatgt agttcctgaa
    31801 ttgccatttg gtacacacga gattgaaagc ggaacaaaaa cgtatcaagt agacgcttca
    31861 aaaaacgata caccttatct ccaagtatca gatcgtcgag gtgtaggtgc agatggccaa
    31921 gcacaagggt ggaacgtgac agtttcggtt tcagattttg taaacggttc acaagttttg
    31981 caaggcgctg aattagattt tggaacatcc actgttaaaa gtacgtcaga taacgaatcc
    32041 actggaccaa ctagtcaaac tgtaactggg ttgagtaaag caagtgcagc gacaccaata
    32101 tttacagcag caaaagatca aggtctggga acatggcttt ctgtttatga tccagcaaat
    32161 atcacactga aagtgccgaa agcagcagct ggaacattca cggctgattt gacttggaac
    32221 ctcgtggctg gcccagtagc atagagtatg aaataaattc aacaaactgc ggtgaaaata
    32281 ctataggtga aggatttttt accgcagttg ttgttgaaag gatgtggtgg acaatgaatc
    32341 aaagtaaacg actgtttgtt tcgttttttt ctattttaag tctatttttc atttttccgt
    32401 caatcagcca agcaaaggat tcagcaggag actttggaat caaaccggta tttccagaga
    32461 accaaataga taaagcaatc ggttattttg atttattagt ggcaccggaa caaaatcaga
    32521 cactagaagt aattatcagc aattcttcag atgaagaacg aacatttgag gtctctgtca
    32581 atccggcggt aacgagtgat ggtggaacga tcgattatag tcaaaaaaac ccaacgttag
    32641 atgagacatt gccgtttgat gtccgtgacg ttttgctcat tgcgaaaaag gaaatcaatg
    32701 tatcagcaca tgcagaaaca actataccta tagaagtgaa aatcccagca aaaagcttta
    32761 agggacgtgt attagcgggt attcatgttt cacctaaaga agaagctgaa acagaaaatg
    32821 ctacagaagg agcacaaatc aaaaatcgta ttgcttataa tctcgcagtc gttttacaag
    32881 aaagtcaaga gacgatagaa ccggatctga aacttttatc aggagatttg gacgaggtca
    32941 acgcaaagcc aactgttcag cttcgtttcc aaaatcctca gcctaggatt atcagcaatt
    33001 tgatatttac gagcaagatc ttttatgaaa atcagcttta tattgaaaat acgtctaatg
    33061 aatttttagt tgcaccaaat tcgaattttc acttgaatct tgatttagct ggtgataaag
    33121 caaaagccgg cgattaccgc gcagaaatca tagcaaaatc aggcgacagt aatgaatggc
    33181 gatttacaca aaactttacg atcaaaaaag aaaaagcaca aaaagtaaat gaaaattccg
    33241 tttttgcagt acaagaacaa tctttcccat ggctttatgc aggattggtt gctgcagttc
    33301 tagtgttatg gttgattttg ctgtttttgt gggtccacaa gaaaaagaaa aaaggagaaa
    33361 caacatgatg aggtgatttc gttgaaaaca tcgatcttat tgggagggat tattgcaagt
    33421 ttgctgttct tcagctggca agagccagtc caagcagata ccaaaagttc aggtacgatt
    33481 acattttatg atctaagtcc agttccgcat ccaagtggtt cagtcaatgg cgcatcaaaa
    33541 cagcagacac agctaccgat ttataaagaa tctaatcaac atggcgtatt cccggagacg
    33601 aatgaagaaa agatccgttt tctttatttg atcattggta tagaattatg gcttcttgcg
    33661 tggttgttgt tatggagaag aaaggaaaag gaggatgaac atgttgctac aaaataaatt
    33721 ttcatttttt cgctccttct tttttacttc gctttttgtt ttaagtatat ttatcttttc
    33781 accttcggca gaagctgttg gagcaacgcc tcctgccatt tcttatgatc catacacgta
    33841 tgaaaatatc gtacctccaa atggtggacg tagtgcagtc aatggcggga agcttgtcca
    33901 actaactggg gctaaccaat tgcctaaatt gactgctact tatttgaacg atggttcaaa
    33961 tgatccttgg aaaggtggca cggtccgttt tcattgggaa agccatgtga aactaacaaa
    34021 tgtacttgat ttggtgaata tcactgcaca aggatttgca tttgtggcga tcatcaattt
    34081 gccggaacat gtatcaagca gtgatgtatt aaaagcaatt gactggaaca acgcctactt
    34141 gtatattgcc ggtgccccca tcaaggttac tagcggtagt ttattatcag caggaagcca
    34201 taccttgcgt tttgggatgg ggtcgtggga tacaaatact ctattggcag atattattgc
    34261 tattattacg cgaggcgaat tcaacgtgga cgatatcccg cttcaatttg atgttacagc
    34321 taatgtcgcg aagatgacag aaaacggaga taaatatgat acgagtccaa acaaaatttt
    34381 gaccaagggt aaattacaac cttctatgtc taaaaaggcc gttttttctg ttgattttta
    34441 tgattcgaat aatattatcc aagatcgtat gttcaattcg attttagctc cagctgcagg
    34501 tagattttat cctaaattaa ctggcggaaa cttgaatcca ggagcatatg cattttctgc
    34561 aagtgcagag ataaatacgt ggaatagtta catctcaaca agtgattcaa caggaaaata
    34621 taatacattt gctgatacca cagaatacgt ggatggaaat aaccagaacc ttccaggctc
    34681 taccaatagt cggacattga cgatggggct gactgaagga cgttttgatc agttgccatt
    34741 tagtcgcttt gatcgcgtag tcaactattt tacaggaaaa aacgttacac aaggagcaat
    34801 actgactcat actccagcaa gcacaagtgg attgaatcag acagccaata tcgtgtacag
    34861 tgggaaagat gcgcaaggcg gattgctttc tcctgttgct ttaaaggtat tggagcagta
    34921 caagctagac ggaacggttt taccaacaaa cttaacagcc gatagtcagt ggggaaagct
    34981 cctttcttct gcatcgtacc aagtcacggc caattggtca gcaactgaac taaaaaaggg
    35041 ttcgatccat tacaggcttt attcgaaagg aacccaaaaa ttagcgacag gatggacaga
    35101 tcaacttttc caaacgatcc aatcaaataa cgggggtgta aatacagcta cttctacact
    35161 acctgcattg gcttcagggc aatattattt tgattatcgc ttggtggacg atgtacttac
    35221 taacatgtat ccgtcttttg cgtacaaatg gcagtctgaa caagctggaa tcaccactct
    35281 tcctcaaata acagtggcta attttccatc tgtgagtgca gatagtcagc taaaaaacct
    35341 tagcagacaa cctgagacag gagatcccct gtctgctcta tcaggagata cgattcaaga
    35401 aaatgtgaca ttcacgttaa caaaatctgg ggatgcgata accgacaaaa aaatcacgat
    35461 ctcacttcct gaaaatacta cttatatcaa tggttcctta aaattaaatg gtacaactat
    35521 cgcagatacc ggcattcaac aaggagtttc tgtcccagca gatctattga gcaaaatagg
    35581 agataccatt catcttactt acaactatca attaaatact gtagatactt ctgtacaatc
    35641 cgtcagcata ctgacaaagg cagctgtact ttccagcaat attacattag cagatggtgc
    35701 taagcttcct aacccagttg tccaaacatc tgctaaaaca attcttgtcc ccaaacaaga
    35761 attaacatta gttaatgtac cagatgattt tacatttggt aacgatctcc ccaaaccgtt
    35821 gaagacaagt tattatgaag caaaaggcga tttttctttt gatgtgcggg atactcggct
    35881 tccaagcacg tctccttggc aactgacagg gacattgact tctttattta agaatgatca
    35941 aggtcaagaa ttaagcggaa caaaattata cttcaatcat tctggatcaa aacaattgat
    36001 tcagcaaggt caaaacacgt tgatttatga gagtgatgga acagctaaag gagaggttct
    36061 cgttgacttt cccgataccg atggtttact cttagaggta aatagcagta cgaatgctca
    36121 acctggcgca acgtatcaag gcatggtcac atgggaactg acagctgggc caaccagcta
    36181 agtgattaga atttgtatgg atctgtgata actgtcacag atcctctttt tcaaggatga
    36241 gaaggaaact tctgctgtta aaatcagaaa tgcttggagg agtggacttg gaacttttca
    36301 gtttgcttga aaaaaatgaa caacggcaaa tcaaactttt ccaactttta ttagaggaaa
    36361 aaggagagat ttccgttaat aacgtactta cccgcttatc agttgatcgg gctaccttga
    36421 aagaagatct gttttattta caagctaact tggaaaatat caaacaattg gaaattgtta
    36481 tccaagaagg gaatatttta ttaatcagac atggaaatat agcagcagtc gatgtttatt
    36541 atcattgcta cttgcgctat tctacaaaat atcagatact tctttatctg ttggaaaatg
    36601 gctcatttga tcggcagaaa cttgtcatgg aacttaatat cagttcggct actttgtctc
    36661 gcagagtgaa ggaattaaat gattctttaa atgagtttca tctgaaaata aaaagcgggc
    36721 ggctgaccgg tccagaaagt caaatttgtt atttttattt ccaattattt tggtttggac
    36781 gtccttatct tgaaaacaaa aaagaataca gtgatttatc aggaaacact tttttagatc
    36841 tgctgcaaaa agagtttagt tttactatta cagaagaagg acgattgaaa tttgaaatat
    36901 ggctttccat tatgaagaag cgaatgcgct gtacgaaaaa atccaaacca gattatagca
    36961 atcttcctgt tggattgttt caaacgacac aatttttatt ggacctccat acatttttga
    37021 cccgttactt ctttcaatat gcctttcctt ggaatcaaaa tgaaacgttg gttttttatc
    37081 ttttcatgat cagtaacttt acattagata cacataactc gcatgtccag gagtttttga
    37141 taagcgaaaa atacaaagac gacgtagtag ctcaattgaa tcgaacgttt aaacaatcga
    37201 tagaagaata tttctctttt tatgtttttc cagaagagat ttcgcaaaag attgcgttga
    37261 cagtgatgca aaatcacgct cattttgcct attttagagg atggatgaat atttttggcc
    37321 aagaaggatt aaaacagcgg ttaactgaag tagaagataa acaattcctg gttttatgtg
    37381 aaagattggt cagacaaaca tttgctattg ttgattttac tgaagtagac gaggaaaatg
    37441 cccgctttga attatttgct cgctatgcat ctattttgcg gacgctgatc aagcagttag
    37501 aattacgtct gcaaattgct tatgatcttc cttatgaaag gctaatggcg gatatcgtga
    37561 aagaaatcat ccaggaacaa ttgctgaata cgatcaaata tgatttagtc gaatacgaga
    37621 aagaaaagca gtatgatatc attttgacta gccaattgaa agagtacccg tcacagaagt
    37681 ccgctaaagt ttttgtattc actagcaacg aatataatta tgatttcccc tatttgaacc
    37741 aatttttaaa agaatgttat ttagaaaaat tgaatttaag gatgaataaa acatgaaaat
    37801 cgtgatcatt ggtgcttccc atgcaggcat cacagctgcc ctaaatctaa gaaaacttca
    37861 accgaaagca gaagttttac tgatcgatga agatcataaa gatggactag gctatgtttc
    37921 aaatggaatc aacctttatt taaaaggaaa gatacgtagt ttagctgaag ttgcccataa
    37981 catgcggact cttcaagaaa gtggggcaaa actgatcacc gaatggagag taactgaact
    38041 tgatccagac aaacatcagc tcatcctcgc atccaaagaa ggaaaaaacg agaccataac
    38101 ctatgataaa ttaatcgtag caacaggttc ctctcctgtc accttgtata aacaaattga
    38161 agctgaaaat gtctacacct ataaaaatct agtgcaatct aagcaagtac ttgccgcttt
    38221 aaaagaagca aaagaagtag tgatcttcgg tgctggttat attggattgg aattagcaga
    38281 tgctttgaga aataaaggat acattattca tctagttgat tatatgccga atgttttatc
    38341 aagatatttc gataaggata tgatcaacag ttttcaaaat cagctgcaaa ctaagcaaat
    38401 caatttttat ccaaatgaat tcttgatcga ttggaaaaaa tcagaagaga aagtagtatc
    38461 cgtacaactt ctatctcaag caataaaggc agatatggtc attttttctg cccaaactcg
    38521 tcctaatacg acattgttaa aagaaaaagt ggctttgtat gaggatgaga ctgtgatagt
    38581 caatgaatat ctgcagacaa gtgatccgga catctatgcg attggagata tcgtcccagt
    38641 cagttttgac aaaaataaac ggcatctctt tttaccttta gttactcgag ctgtacatat
    38701 ggcaagagca gtagcgctaa ctttgagtgg tcagccaacc gcttacgacc tcagacaaaa
    38761 gataacagca gcagtcatta tagactactt tttaggaact gtagggctga cagaagatga
    38821 agcgccattc ttggaacaaa ataccggttc gtgttcagga gaatttgatc tatttccaca
    38881 atacgatgaa gaaaacaaaa cagtaaatgc aaagctgatc taccatccgg atacattaga
    38941 aataattggc ggacagctga taagccaaga attcttatta tcagatttga acctgcttgc
    39001 ggatatcgtg aaacacaaaa ctacgattcc acagctagca gttgaggatt ttggatttct
    39061 gtctgaacac acctctcgtt ttcactacct caatgagtta gcttttaaag tactagctaa
    39121 gagagcaaat agcaatagag tagataaaag actgtgagaa atatcacggt cttttattgt
    39181 gtggggatat aataaatatt aatcaatcga gaataaaaaa ttcatataat gaaatagcgg
    39241 aagaattttt tgagcataat attcgccaaa aatttatcaa gtgataaaat tgttgtataa
    39301 aatgattatt tcaggaggag gagttaaatg tttttagcat taaatgaaat tatgcattca
    39361 aagttacgtt atgcgttagt tgcaggggtg atgtttttga tcgcctacct ggtatttttt
    39421 ttgactgggt tagcttatgg cttagctcaa gataatcgaa cagcagtcga caagtgggag
    39481 gcagattcga ttgtgctttc gaaagatgca aattcaaatc tagggatgtc catgattact
    39541 aagaagatag cagaagaagt cgaaggtggg aaagtggcgt atcttgccca gacacctgga
    39601 gtcgtgacta gtaaagacag tacagaagaa ggaaaaatca atgttagctt cttcggtatc
    39661 gataaaaatc aattcatcat gcccaatctt gttgaaggaa aagcttttga taacgatgat
    39721 gaggcagtag gcgatattag tttgaaagaa gaatatggtc tagccgtagg agatacagtt
    39781 aagttatctg gtagtgacaa gacgtttaaa ctaactggct ttacggatca tgccaaattc
    39841 aatgtctcac cagttctcta tacaaccatc aatgcttacc aagagatccg cttcgaaaaa
    39901 gaagatacaa gtgaaaatgc acggattaac gcaatagttg tcagaggtaa aatcagtaat
    39961 ttacctgaag atttagaaca gatcaaaatt tctaaattca tcaatgaatt acctggatat
    40021 aatgcgcaag tattgacttt tggatttatg atcggatttt tgatcgttat tgcggctatt
    40081 gtgatcggga ttttcattta tgtactgacg atgcaaaaga ttaatatctt tggtgtaatg
    40141 aaagcacaag ggatcacagg aggatttatt gcgcgttctg tagtggcaca aacattcatt
    40201 ttatcatttg ttggtattct tcttggtcta ttaggaacag tgggaacatc ccttgttcta
    40261 cctgatgcgg ttccattcca aagcaattgg ctgttttttg gcgtcattag cttgttgatg
    40321 ctggtagtag ctgtgttagg cgcactattt tctgttcgga caatcgtaaa aatcgatccg
    40381 ttgaaggcaa ttggataaga aggaggaaga gcatgaaagc aatagaattt aaagaagtag
    40441 aaaaaagttt ccaagatggc gaccaagtag ttaaagcatt gaaagaaaca accatgagta
    40501 tcgataaagg ggaatttgtt gcaatcatcg gtccttctgg ttcaggtaaa agtacttttt
    40561 tgactttggc aggcggattg cagacgccta gcagtggctc tgtatttatc aatgatcaag
    40621 aattcagtaa tcaaaaagaa agtgaacgcg ttaaactacg ttttaaagaa attggcttta
    40681 ttttgcaagc ttccaatctg gttccttttc tgacagtaaa aaagcagtta ttattagtag
    40741 ataagatcaa acatgaacat cgtcaaaaag aagcaaaaga attatttgag cagcttggcg
    40801 tggataagtt gatcaacaag tatccagaag aactatctgg tggagaacgg caaagggtcg
    40861 caatcgctcg tgcattgtac aatgatccgt ccattattct agcagatgaa ccaactgcta
    40921 gtttggattc agaaaaggca cgagaagtag tcgatatctt ggcaaaagaa tcaaaagaca
    40981 aaaataaagc aacgatcatg gttactcacg atacacggct gattgacaaa tgcgatagaa
    41041 tatttatgat ggaagatggt gtattgaaag aaaaagaata accaaaaagc aggtggaaaa
    41101 atggtccatc gatacaacaa agatacacag caaaaaattt tgaaagaagc gactgacttg
    41161 tttatgtcca agggatatct cggcacttca acaagagaga tcgcacaaaa agttgggatc
    41221 acccaaccca atctctatca ttatttcggt gataaagaaa aactatatac ggcagtttta
    41281 gaaaaccact taaaagatgt tggaaaagct ttgagagaaa tcgttcaaac aagcgaaacg
    41341 ggatttcaag taaccttgac aaaaatggcg cagtttttga ttgagacaca tctggtcgat
    41401 ttgtttctga tgcttcacga tcttgaaagc aatctttcaa aggaaacaag agatcatctt
    41461 ttctttctat ggaagaaaaa ttatcgtgaa ccttttgaag aaatcttttc agcaaaccaa
    41521 tctgtgctaa gaaatgggat ttcaaaagaa atcgcagcaa ggcatttttt cttagtgtta
    41581 gcaccttaca tcactcggtc gaaaagacct gtggaagaac cgctaaatgt ggcgcaattg
    41641 atcgatttat atttgaatgg tgtattagga gaagcgtaaa gctgttatat agggagaaaa
    41701 atagttaggt gaagaagcaa gatatgacga agatcgttat atcttgtttt ttttatatag
    41761 gtagtttttc ataaatacaa atttgttatt ctctcatgat tatcagggta tatttttatt
    41821 tattatgtcg gtgttccagg aagtgtttta atttaataac gaagaaatta attgtttgta
    41881 gaatgaagga aactatttat tgggtagatg aatcaccacg aatagtcaaa aggagatttc
    41941 aggatatgcc aaaaatgaaa aaaatcattt tagcagtaat gttattttct acggttatat
    42001 cttttacagc cccttttttg cgtacttaag ctgattttaa gttttgaccc ttagtctgga
    42061 tctacgaggt gagagtcaag aatgaaaaaa ataattggag cttttgtatt tgcaatcatt
    42121 atcattatta tctgttttca aatagatgtt tttggtcaaa aaaagatagt tggacaaacg
    42181 gtcagcagta cgaacagtac ggttacaaag acaaaggaat catttgatga tacagaacaa
    42241 gagacattaa tcaaaatcgg aaaaaatgac cgtacactat atacagatga ctcgctaact
    42301 accccacttg caactataaa cggcggagag ctggcggagt ttctttctga aacaaaaaat
    42361 gcttatcaga tcaagacaaa tgatggctat actggatatt tagccctcga agatggaacc
    42421 aaagtcacaa agaatataca aacaaaaccg gaagagctat ctgatgcagt gattgtatta
    42481 gatcctgggc atggcggaaa tgatactggg gcattaagca atgatgaaca aacagaagaa
    42541 aaagatataa cattaagtac agccataaaa gtaaaaaagg cgctagagga tgctggagca
    42601 acggtttatt tgacccatac tacagatgaa ttagtacagc ttggagatat atgcgactac
    42661 agtgaagaaa aaaaggcaga tgtctttatc agtttacacg cagactctac ggaatatgcg
    42721 aatgaagcaa ctggaatcac gacatattat tactatggac aagaggaaac attagcacag
    42781 acaattgctg actccttcac tgatctgcct ctggattcaa gaggaatctc cactgggaac
    42841 tatcaagttc ttcgagaaaa tcttcaacct tctattttaa tcgagatggg ttatatgaat
    42901 aatgatagtg atttaaagga attagtaaca gacagttatc agcagcagat tgctgcaagt
    42961 ttgacacaag cattgaccac ttattttcag taataaataa gcccagccaa acaaaaatag
    43021 ctcctcatat ttttcgtttg gccgggctta ttatcgcaga tgcaagcttt tgaacaccgt
    43081 ttaaggaaga ggttgtgagc ctgccgttta actccgcgtc acaaggagca atttcaaaaa
    43141 acagcttctc atgtttgatg actattgtga cttgtgaccg aggagttggc atgagaacac
    43201 cgtttattcg gaacaaggga ctgtgacatg acttttgtca cagttccatt atttttttag
    43261 cagcttcaaa gctttttctg tattggcaaa atgaagtttg gcatattttt caagcatagg
    43321 catgccgcct ttctccaata gttttgccgc tacttggtca gatttcgttc cagctcctga
    43381 tggaagggtg attccagctt cagctgatgc tttgtgtaac tcttccaaaa agctggcacg
    43441 actgatgata gaagcagcag caacagctaa atgatattgt tctcctttag taacgaagta
    43501 cagtttttct tgtaattgat ttttttctaa acgcgcatat tttttaaaat ttgcttccgg
    43561 cgtaaactga tcgatcaaaa tcgcttctgg tttttctggc tcgatttttt ttagcagcaa
    43621 atggatcgct tgattgtgca aagcgacttt catccgaacg gcattgtatt ttggttggat
    43681 ctcattgtat ttttctggag ataagacaag cagtttatag gggatggttt tttttaagat
    43741 gtcagcaagc tggatgattt ggctatcttt taactctttt gaatctctaa caccaagttt
    43801 acgtaattct gcaagctgat cacgtttcac ataagcagca catactgtta gcggaccaaa
    43861 atagctgcca tttccgacct catcactacc taaaacggat aattgactga tattttttgg
    43921 caaagaagag gctgtttttg ctggcttggc ttgtttatcc tgtgcctccc cccatttatc
    43981 agcttctatt gctgcattcg taccttgaaa catcactttt ccggatgtat aagcggtaat
    44041 ggtcgtcccg tctttttttg cagcaaagct agtataagga atctgtttat ttaacaaata
    44101 agagcgatag tatgctttca tttgactaag ttggtcgttt gttgctttga taatgacgtt
    44161 gctcataaaa ttctcctcgt ttttatacat aaaactatac cgcaactcac gacttttcgt
    44221 tttctaagag attgcgagac atagcatttt tttaaagttt atcatataat agttaagtat
    44281 gtgaaaaata tttgtggaga acggtgaaag aaccaacttc atggtacact aaataaaact
    44341 ttcatcaaat aggaggaaat gattcgtatg gcgcacgaaa aaacacgcta caaagcagtg
    44401 attgccaacc aaacatatac tattatcgga cgggaaacca agcatcatat ggatatcgta
    44461 acgaagctga tcaatgaaca gttagcagaa ttgaaacaat tatcaccaca aatggataat
    44521 gaacaagcag caatcttgat ggctgtcaat gcgctttcgg atcaattgaa gaaacaagaa
    44581 agaatcttgg agctggaaga agaaacagca gaattaaaga aaaaaatgat caaatttaca
    44641 gagcttgaaa atcgtgtgaa acgcattgaa gcaatcgaga acgaagctcg ggaagtattg
    44701 aaagaaaatg gtcaagcaga tcataccatt aaaaatcatg tggaagcaca acagatttta
    44761 aacgagaaac gaaaaggaca aattaaacaa aaatcttcta attaagatcc ttttatcaaa
    44821 ataagaaaaa tttaaaaaag ttagaagaga aaggaacgta gcatgctgag tttgctgatt
    44881 ttatttttat tgctaattgc gtttttttct ggagcacgtc gcgggtttgc tttgcaaatc
    44941 atgttcgcga ttggatatgt attgtcattt attgctgccc agcattttta caaaccatta
    45001 gcaagtcatt tggaattata cattccatac ccagctgtca ccccaacatc aaagttagca
    45061 ttttttgatc aggtatttgc ttttcaccta gatgaagctt tttatgcagg aactgctttt
    45121 ctgattattt tgctaattgg ctggctgctt acccgatttg tgggggtttt tgtccatgga
    45181 ttgacgtatg tgccgatctt gagacaggta gactggattg caggaggcat cctgtcacta
    45241 atcatggctt acgttactat atttttgatt ttgcagttac ttgcctttgt tccattagat
    45301 gtggtccaaa atcaattcgg cgagcatagt ttcgctcgtt ttatcgttga gagaacgcca
    45361 tttcttacca acaagatgta tgagctttgg taacgaatat tttgaattaa ggaaaatagt
    45421 gctggaaaat atttcgtttt ttgtacagag tagtttattt aagaaggctg cgtagcgtat
    45481 gtccggcaca gctctttttt catagtgaaa gataaggtga taaaatgaat aaccgaatcc
    45541 ttgaaacatt ggaatttgaa aaagtcaaac agatggtcag acaatttgta gtgactgcac
    45601 aaggaaaaga agaactggca gaattggtac cagtgagtga gaaacaaacg atcacaaact
    45661 ggcttcaaga aactgaggat ggattgaaag tgcaacgtct gcgaggcggg atccctatac
    45721 ctaaacttga aaatatccgt ccccatatga aacggatcga gatcggagca gacttgaatg
    45781 gagtggagct tgcacaagtt tccagagtat tgtctacgac aagcgaattg aatcgtttca
    45841 ttgatgatct ttctgacagt gaaatcgaat ttgcacgttt gtatatgtgg gcagatcagt
    45901 tagtcacgat ccctgttttg agccgccgtt taaaggaagc aatcgatgaa gacggacgcg
    45961 tgactgatga cgcttctcct gagttaaaga gcataagaca gaacattcgg cgaagtgaac
    46021 aagctgttcg tgaacagtta gatgggatcg tccgtgggaa aaatgcaaag tatttaagtg
    46081 atgcgatcat caccatgcga aatgaccgct acgttatccc agtcaaacaa gaataccgcg
    46141 gtgtatttgg cggtgtagtc cacgatcaaa gtgcttcagg acagacttta tttattgaac
    46201 caaaacaagt ggtggatttg aataatcgtt tgcgtcaata ccaaatcgct gaacgaaatg
    46261 agattcaacg tattttaagt gaactttctg ctgaacttgt tccccatcgt caagaaatca
    46321 tccacaatgc ttatgtgatt ggtaaaatgg atctgatgaa tgcaaaagca cgatttggta
    46381 aagaagtaaa agccatcgtg ccgggaatca gtgaagacaa tcatgtcgtc ttgaaacaag
    46441 caagacatcc actgatcgat caggaaaaag tagtttctaa tgacatcaca atcggaaaag
    46501 actatcaagc catcgtcatt acaggaccta acacaggtgg gaaaacgatc acgttgaaga
    46561 ctttaggtct gttacaactg atggggcaag caggattacc gattcctgca ggtgaagaaa
    46621 gtcaaatcgg tatttttgag gaagtatttg cggatatagg agacgaacag tcgattgagc
    46681 aaagtctatc gaccttttct tctcatatga cgaacactgt agatatttta tcaaaagtca
    46741 atgaaaaaag tctggtatta tttgacgagc tcggtgcagg aacagatccg caagaaggtg
    46801 ctgctctagc aatcgcaatc ttagatgatc ttggtaaaaa gtctgcttat gtgatggcaa
    46861 caacgcatta tcctgaattg aaagtttatg gatacaatcg ggcaaatacg attaatgcca
    46921 gcatggagtt cgatgtggat acactaagtc caacgtatcg tttgctgatt ggcgttcctg
    46981 gacgaagcaa tgcgtttgaa atctctagcc gattaggtct tgatacagaa gttattgatg
    47041 aagcgaagca gttgatgaat gatgagagcc aagatttaaa cgaaatgatc acagatttag
    47101 agaatcgccg gaaaatggca gagaccgaat atctggaaat gcgtcatttt gtttctgagg
    47161 cacaagaact tcacgatgat ttgaaagaag cttacagcta tttctttgaa gaacgtgaaa
    47221 aagaaatgga aaaagcaaag aaaaaagcaa acgaagtcgt gtcagaggcg gaagaaaaag
    47281 cagaaaaaat cattgctgat attcgtaaaa tgcaacagca gattgggcaa ggaaatgtga
    47341 aagagcatca gttgatcgat gcaaagaccc aattagcaaa tctacatcaa gaagaaactt
    47401 tgaagaaaaa taaagtattg aagaaggcga aagaacaaaa aacgttgaaa ccgggagacg
    47461 aagtgttggt tacgacttac ggccaaagag ggacgctttt acgtaaaaac gggaatcagt
    47521 ggcaagtaga aatcggtata ttaaaaatga atgtgtccga agacgagctg acgcctgttg
    47581 ctcctcaaaa agagccgaca caacgagtta tccatgctgt acgatctgaa tctagtagtc
    47641 acgtgccgaa tcaattggac cttcgaggaa aacgatacga agaagcgcta agtgaggtag
    47701 atcaatattt ggattctgcc attctagctg gttatcctca agtgactatt gtccacggaa
    47761 aaggaacagg cgcacttcgc aagggaatta ctgattatct taaaaaccat cggagcgtca
    47821 aaagctttga gtttgcaccg gcaaaccaag gcggaaatgg tgcgacaatt gttaaattta
    47881 agtaaagctt actaaaggta agcagatggt tcgctatcca agtatagaaa tgctatacta
    47941 atcatagatt ttaaaaatgg aggtctttga tatgtcacaa gtaattacag ataagacatt
    48001 taatgaagaa acagataaag gacttgtatt gattgatttt tgggcaactt ggtgtggtcc
    48061 atgtcgtatg caagcaccga ttttggatca gttggaacaa gaatatgatg aagaagaatt
    48121 cagaattgct aaaatggatg tggatgaaaa tccagaaact cctcaacaat tcggtatcat
    48181 gagcattcca acattaatgt tgaaaaaaga tggtcaagta gtagaaaaag ctgttggtgt
    48241 tcattcaaaa gaacaactac gtcaaatgat cgatcaatat ttataaaaac ttacagcaaa
    48301 aataagctga gggtgggact gaatccagtt ccgtcctcgg tttttttgcg gttgagttaa
    48361 ggttattcaa attaatattt tttgagtata attacgttgt caggctggat tgtttttttg
    48421 acagacatgc tataataact ttaattgaat atgaagggga gtaccagatg aacgaacgaa
    48481 tcaaaaacaa attagcttta cttcctgatc agcccggctg ttacttgatg aaagataaaa
    48541 atggaacgat catttatgtc ggcaaagcca aaatattaaa aaatcgtgtc cgctcatatt
    48601 ttactggcag ccataatacg aaaacggaac ggcttgttag tgaaatcgaa gactttgaat
    48661 acatcgttac agaatcgaat atcgaagcat tgctcttaga gatcaatctg ataaagaaaa
    48721 atgatcctaa atacaacatt atgttaaagg atgataaaac ctatcctttc cttaagataa
    48781 cgaatgaaaa atatccccgt ttggtgatta ctcgtaaagt actaaaagac aaagcactgt
    48841 attttggacc atatcccgat gtcggtgcgg cgaatgagac aaagaagatc ctcgatcgat
    48901 tatttcctct gagaaaatgc aaacctagtc agacgaagga gccttgtctt tactatcatt
    48961 tgggacaatg tctgtgtcct tattatttcg acgtagatcc gaaagtctat acgaaaatcg
    49021 ttgatgaagt gaaaaagttt ttaaacggag gacacgaaaa aatcgagtca gagatccatc
    49081 agaaaatgga aaaagctgct gaaaacatgg agtttgaaaa agctgcagaa taccgagacc
    49141 agatccgtgc cattgagaca atcatgactc gccaaaaaat gacaaatact gatttactgg
    49201 atcgagatgt atttggttat gcgattgaca aaggatggat gtgtgtccaa gtgtttttcg
    49261 taagacaagg aaagctaatt gaacgagatg tttcaatgtt tcctttctat aacgaagcag
    49321 aagaagattt cttgacttac ctcggtcagt tttatcagga aaatgagcat ttcattccta
    49381 aagaagtctt gattcctgat acgatcgaca agcccagtgt agaagctttg ttagctacga
    49441 aagtgctgca accaaaacga ggagaaaaga aaaaattagt tgaattggcg aataagaatg
    49501 cctcagttgc tttgaatgaa cggtttgatc tgattgccag aaaacaggaa cggacgatcg
    49561 gagcagttga acgtttagga aacgcgatga atatcccgac cccaatacgt atcgagtctt
    49621 ttgataactc taatatcatg gggacgaatc ctgtggctgc gatggtcgta ttcattgatg
    49681 gaaaaccggc aagaaaagaa tatcggaaat ataaaataaa aacagtcaac ggaccggatg
    49741 attatgcatc aatgagagaa gtcatctatc gcagatattc acgagtgatt cgcgaagggt
    49801 tgcctttacc tgatttgatc ttgatcgatg gaggaaaagg acaagtagat gtggcaaaag
    49861 atgtcttaga aaatcaatta ggaatcgata ttcctattgc tggcatggca aaaaacgata
    49921 aacataaaac aaatgagttg ctattcggta tagatttggc tgtagtaccg cttgagcgaa
    49981 attcaccaga gtttttctta ctccagcgaa tccaagatga agtccatcga ttcgcaatca
    50041 ctttccatcg gcagctacgc agtaaaaaca gttttgcttc ccgattagat gaaattgaag
    50101 ggttaggacc aaaacgaaaa aaaatgctgc ttaaagagtt caaatcgttg aaaaatatca
    50161 cagcagccac gacagaagaa ttacaagaaa tcggactgcc gaaaaatgtc gcacaaaacg
    50221 tatttgataa attacatcag gaatgaggaa agggcacttt acaaagaaag taaagtgtcc
    50281 tatcatttta tgtacaacaa aaagagcgtt tacgttttgt tggtaaataa taaatcgctt
    50341 acgtctggtg tctaaggata tatgtgattt cttgaagaag gagtgaagaa atgttagaag
    50401 tcaaggatct tgttaaaact tttgggaact acacagctgt cgatcatgtc tctttccaaa
    50461 tccccgatgg aaaaatcatg gggctaattg gccaaaacgg ggctgggaag acaacgactt
    50521 ttcgtttgat cttggatttt ttgacacaag accaaggaga agtgttatgg aatggccatc
    50581 atcttgggga gaaagattat gatgtcatcg gttatcttcc ggaggaacgg ggactttatc
    50641 cgaaagtaac gattcaagac caactgcttt attttgcaga acttagaggg aaatcaagaa
    50701 aagagattga accaaaaatc gatttttgga tggaaaaatt tcaggtaaaa gggaaaaaga
    50761 cggataaagt aaaatctttg tctaaaggaa atcagcaaaa agtccagctg atcgccacgt
    50821 taatccatga acctaaattg atcattttgg atgagccatt cagcgggtta gatccggtaa
    50881 atgctgaatt gctaaaagat ggaatattgg aattgaaaaa acatggttca tgtgtcattt
    50941 tttctagcca caatatggat aatgtagaaa aaatctgcga tcatttgatc atgttgcgaa
    51001 acggaaaaat ggttttgaat ggaaaagtac atgagattcg tgaatcattt ggccgtacga
    51061 agttattctt ggaatctggt ttaagccaac aggaagtaga agaaatcgaa ggcgtgaaaa
    51121 aagtgatcct tcatgaagac aatacgttag agattacctt agaagatcct gaagctggga
    51181 aagaaatctt tactcgtgcg actcagttcg gctacattcc tatgtttaac cagcaaccgc
    51241 caactttgga agagattttc aaattgaaag caggtgaaat cgatgcgtaa gttttggatt
    51301 attaccaaag atgtctattt aaaaaatgtt aagtcgattt ctttcttgat catgatatta
    51361 gtccctttta tcatgctggg tatcatttat gtagcaggaa actttgctca acaaaatagt
    51421 gatgtcaata aaatcggcgt attggctgat aatgagcaaa tcagtcagca aatgagccaa
    51481 gtcaaaacag atgattttga gttcaaagca gttgattctg aagaaaaagc acaaaaagag
    51541 ctttcggaag aaaaaatcga tgcgtattta gtagtctcaa ctgagaatga taagataaat
    51601 gggatgcttt atagcgaaaa ttcattagga caaagtacac aactactgat acaacaacaa
    51661 ctgactggct tgcaatcgat gcttcgagca agtcaattag gcatttcacc agaagaagta
    51721 gctagcttga gccaacaagc aagtttgact cgacaaaaag tcaattttga ttcaaaagga
    51781 aaaatgacag ttggagaaga taactcgaca gtccaatatg tcgtaagtta tgtagcaacg
    51841 attgtcttgt tcattattat tttgacgtat gcgcagatca ttgcacaaga gatcgcttct
    51901 gaaaaaggca cgcggatcat ggaagtgatc ctgtccagta cgactgcaca aaagcatttt
    51961 tacggaaaac taacaggtgt tttacttgtt gcattaacgc aaatggcatt atacggtatt
    52021 atttttggta taggatacag tcaattcaaa gatatggaaa tggtcaaaaa tatattagac
    52081 ggtatctcat tggataactt gattggacca ttcttgtggt attctcttct attcatgatc
    52141 tttggcattt tgattttttc tgttttagca gctttatgtg gctctttggt caacaaagca
    52201 gaagatacag caaaagcaat tttgccagtg acttatctat ctctaggcgg atatatgctt
    52261 ggattgatac taggagcttc agatccaaac aatatcgtta ttcggatcac ttcgtatatt
    52321 ccattcttat cttcatatat tatgccggtt cgcttagcaa atgagacagt cggtatgggt
    52381 ggagcattga tttccttgtt ggtacttgta gccgtaacat tcttattgat gatcttgtct
    52441 gcaaacatgt acaaatcgaa tgtactcgtg tatagcgaag gcggattatg gagttcattg
    52501 aaacaatcga tttcaattat gagaaatgaa cgaaaaaaag cataaaatat agaaaaatat
    52561 agaaggaagc tggacattta ttgttcccag cttctttttt gtatagacct atttctattt
    52621 ttttattata ttggatatat atattcattg aaaatagagc ataacaactt tataatgttg
    52681 agtataccaa tttttggagg cgttttaatg gaccgctatt tagatttact ttcagagaag
    52741 ttccctacaa cggaagatgt catcacggaa atcatcaatc tcgaagcaat tttgcagctt
    52801 ccaaaaggaa cagagttatt tttgagcgat atacatggcg aatttcctgc ttttgatcat
    52861 attctgcgta tcggttcagg aaatcttaaa gaaaagatca aagacctttt ctctgaacaa
    52921 atgacggaaa cggagatcag ccaattcact atttttacgg cttatccaga atatgtgttg
    52981 acgactgatt ggtacaaaaa acaagataaa tcccagctga tccatcgttt gatcgatctt
    53041 ttacggttta cgacagtcaa atacactcga tcaaaagttc gaaaaggatt gccgaaagaa
    53101 tatagctata tcattgagga actcttgtat atcgatgatc gtatcaacgg gaaaaaagaa
    53161 tatgtaaaaa aaatcattga acaacttgtc ttgatgaagg aagaagaaaa atttttaaca
    53221 aaacttgcgc agacgatcca aaaatctgtc atcgatcatt tgcatatcgt gggtgatatt
    53281 tttgatcgag gaactcaagc agccaaagta atggatcgaa tcatggattt ttcctctgta
    53341 gatatccagt gggggaatca tgatattttg tggattggtg cgtattgcgg ctctgaggca
    53401 tgtttattga cgcttttacg gattgccgca cgctacaact atttatttga attagagaaa
    53461 gaatatggat taaatttacg tccattattt tcttttgccc atcaaaccta tcagaagaat
    53521 ccagtattta cccctaaaaa caggaaagat ctatctgaaa gagagcaaga agaattagaa
    53581 aagatccatc aagcactttc tatcctccaa ttcaaatcag aacatcagtt gttgaatcga
    53641 cgacctgaat ttaaaatgga cgatcgtaaa ttgttggaaa aaatcaacta tgaggaatca
    53701 acgatcgttt tagaaggtca gctctatgag ttgaaaggga cttgttttca aacgatccag
    53761 ccagatgatc ctaaaaaatt cttggaagag gaacaaaaag tgatcgatag tttgatgtat
    53821 tcattccagc actcgttaag aatgcaaaaa cacatgacgt ttttgatcga aaaaggggga
    53881 atgtatctca cgaacaatca gcatgtactg ttccatggct gtatacctgt agatgaaaag
    53941 gggcagctct tagcatttga attagaccaa tcttatcgcg gaaaagaact gctgggattt
    54001 tttgaaaaac agataactga aagcgcaaaa gatttgactg caaatgaaga tttggcgacc
    54061 gatttgattt ggtatgcgtg gagcgggcca ttttcgcctt tattcggcaa gagtaaaatg
    54121 gcaacttttg agcgctattt tctcacagag cctcatacgc acgtcgaaaa aagcaatccc
    54181 tattatcact tgcgagatga agagtggttc agtgaaaaaa ttttggcaga attcgatgca
    54241 aaagaagaag gttcagcaat catcaatgga cacacgccag taaaagtaaa aaaaggagag
    54301 tcaccgatca aagcaaacgg aaaagcattt gttattgacg gaggtctatc caaagcttat
    54361 caaaaaacaa ccgggattgc tggctactca ttactatgca attcttacgg tttccaaatc
    54421 gtgacacatt atccgttctt gcctattgaa caacaatttg caaaaaaatc tgatcaaacg
    54481 aatgtaaaaa aagtgatcga gcagcagctc ccacgcaaac ttaatcggaa tacgacaaaa
    54541 ggaatggaat tacagcaaca gattactcaa ctccaaaggc tgttggatta tcgaaaggac
    54601 gattgagaaa aatgctagcc aacattgctc aatagactaa aaagcgactg caacaaaagg
    54661 cgtgtcgcag tctccttcgg aattagctca aaaaacggta aaatattaga agctattttt
    54721 ctgtttattt ctctaatact caggtcaggg cgctgatttc acgacctctt ccttaaacgg
    54781 tgttctcctg ccaactcctc ggtcacaagt cacaatatcc aacaaacatg agaagctgtt
    54841 ttttgaaatt gctccttgtg actcagagtt gaacggcagg ctcacgacct aatcagaaaa
    54901 acgattaaaa ttcattcaca aagaaatcga ataaacgctg ttcagatgaa tctacacgat
    54961 gagaaagttc cggatgccat tggacaccaa ggattttgtg catattatct ttactttgga
    55021 ctgcttcaat gagtccatcg ttggaaaagg cgatggcttt taacaaagga gaaagatctt
    55081 tgactgcctg atgatgatag gaattcacaa agtatgaatc agggagaagg tcacttaata
    55141 aactgtccga aacgattttg acttcgtgag ttgcaaactg cggggcagtg ggctgttgtt
    55201 cgtgttttac ggaccatttt ggatataatg aaagatcttg ataaagtgtt ccctccaaca
    55261 cgacattcaa tagttgaagt cctcggcaga ctgcaaaaat cggttttctt tgtttcaatg
    55321 cttccaagat caatgcctgt tcgaattggt ctcgttgaat atttgtttcc gtcagttttg
    55381 gatgagggtc ttccatgtaa agttgcggac taacatcctg accgcctgca agaatgagtt
    55441 tgtcgatttt ctcaattagt tgaggcgctg aatctggaga catgactggc agaataagag
    55501 gtaatccttg agcatctaaa actgcatcca caaatccctg cggtgtatag cttacttgat
    55561 ttccttggaa agtatctgtc gctcgaatca gttgattccc ggcaatgccg atgattggtt
    55621 tcatttttat tccccctaaa gtagactaat tgtaacaagg atttaaaaaa atttaaacta
    55681 tgtaagactt gcccctatac ttatctgttc ttgctgtgtt actatctgta aagagaattt
    55741 tcgtgtgaaa ttcatgacga tagttgaaaa tattgcttaa tttgttataa taaaaagtaa
    55801 gcagaaataa aaaggttggg cgtaagcttc aaggatctag ttcttcaagg ggtgagaagg
    55861 acattttatt gaaggggcct gcctatacta agttatcttt agaggtacat actttcaatg
    55921 aaaaaagaaa gcatgtacct cttttttaaa tcgaaaggaa gtgaagcaag tgttagaagc
    55981 tttggaacaa atccgaaaag cagaagaaga aaatgagaag cgtgctcgaa ccctacggca
    56041 gcagttgaag acctatgcag aagaaaaaga gcaagaattg gagataatcc aaacgaacag
    56101 tcggcaacag ctgaaacagt tggtagaaga aaaagaacaa ttcgaaaagc aagaattaga
    56161 aaaagaaaaa atgaaattga aagcagaagc agacaaggtg caagcaactt tgaaagacca
    56221 atatcaaaag catcagagtc aagcaatcgg tgcaatcatt gaaagggtga aagaaactta
    56281 tggccgtcac taagatggaa aaagtgaccc tcatttcaga taaaagaaat caggagagga
    56341 ttttacaagc agtacaagga atccaaaatg ttgaaatccg ggatctgtat caaaatagaa
    56401 caaacaacca gtgggtagaa aaatattttc cagataccac gattattgat aaagaagcaa
    56461 aattaagcgc tttaggcaat cggctgaaag acatccaaga agctatccaa ttcatacaac
    56521 accacggcga tagccgtcaa aagaaactcc atttgaaacg tcaagaactt tccttacaag
    56581 acttggaagc aaattattcc gaagaaaatt tcatgaataa attagaagaa gtccttgatt
    56641 taaaagaaaa gtgggaacag ctaactgaaa aacgtgatcg tttagaagaa gaggaagaat
    56701 ggcttcttca ttggcaacag ctggatattg ctccaagtat gtatgcaagt aagacgacca
    56761 tttttgagat ggggactgtg agtgcagcaa attttgaaag tttcaaggaa gaactaagta
    56821 agcttgatga cgtttatatg gaagaaatag attatacgcc aaaacaagtt tatctttcgt
    56881 atgttgcttt aaaaaaatca gttgctgcta tagaagaaat tgctgggaaa tacgggttca
    56941 ataaagaaac gtatccttat gaacaaaatc ctaaagaaaa attgcaagaa accaaacagc
    57001 aattatccat tgttcacgag cagcaaaaaa agataacgac agcgttaggt cagtgcagcg
    57061 gctatatcca agaatttgaa tgggtagaag aagtcacact tgccgttgca gaacgggaaa
    57121 aagtaaaaga acggtttatc catgccactt atctgatcgt tttgcaaggt tgggtagatg
    57181 cagaagaaaa acagaatttg atgcatgtgt taaacgaaac tgtatcagaa gaagatatat
    57241 acgttagttt cgaaacacca aactcagaag agattcagca ggaagtccct actaaattga
    57301 aaaaccatcc gcttatcgaa ccttttgaat tgctgactga aatgtacagt ctgccaaaat
    57361 atgaagaagt agatccaacg ccttggatga caccatttta tcttgtcttc ttcgggatga
    57421 tggtcgcaga tgttggttac ggattgctga tgctcattgg gacaatacta gctcaaaaac
    57481 tatttgtttt accccgcggg atgaaacgtt tcgtaaaatt ctttgagatt ctctcggtac
    57541 catcgatcat ctggggattg atctacagtt catttttcgg acaggcatta ccaaaagaac
    57601 tatttggtat ccgcttgcct ttcccaatcc tatctacgac ggatgacgtc aatacgattt
    57661 tgattttatc cgtgattttc ggtctgatac agatcttggt tggattattc gtggcagcaa
    57721 aggaaaatat caggaggaaa gattacttga gtgctgtcag tgatggtttt gcctggcaag
    57781 ggatcctgat tgggatcgtt attgcattga ttggtgtcat gttgataaaa aatacagcat
    57841 ttgtttatct tggcgcgggg ctatcgatca ttgctgcttt atgtatctta atcgtgccga
    57901 ttatccaatc gccgtctaaa gtcaagggtg ctgccaaagg agcgtataat ctttacggat
    57961 tgactggtta tatcggtgat ttagtcagtt atacacgttt gatggcacta ggtatttcag
    58021 gcggaagtat tggcgcggca ttcaatatgc tagttgcttt catgccgcca gccgctcgat
    58081 tcagtgtagg gatccttttg atcatcgctt tacacgcact gaatatgttt ttgacactgt
    58141 taagtgccta tgtccatggt gcacgattgc agtatgtcga attttttgga aaattttata
    58201 caggaggcgg acgtgccttt gacccgctga aaacggcgga aaaatacgtc aacatcaatc
    58261 ataagaaaaa aaacaataaa taattggagg atttttatac aatgatggat tacttaatta
    58321 gtcaaaatgg tggaatggta tttgcagtat tagcgatggc aacagcgacg attttttcag
    58381 ggattggttc agctaaaggt gttggaatga caggtgaggc agcagcagcg ttaacaacaa
    58441 gtcaacccga aaaattcgga caggctttga ttctgcaatt gcttccaggt acgcaagggt
    58501 tgtatggatt cgttatcgcc tttttgattt ttatcaactt aggaaatgat atgtcagttg
    58561 tacaaggatt gaactttcta ggcgcttctc tgccgattgc ttttacaggt ttgttttcag
    58621 gtatcgccca agggaaagtt gcagcagcag gtatccaaat tttagcgaaa aaacctgaac
    58681 atgctacaaa aggaatcatt tttgctgcga tggttgaaac atatgcgatt ttaggtttcg
    58741 ttatttcctt cctattagta ttgaatgcct aagcataaag gaggaaaagc agatgaacgc
    58801 cattgaaaaa atcatctctc agatgaatga agcagcagag caagaaagag cggcgttaga
    58861 gcaagaagag cgaatgaaga tcgatcaaaa ctttgaacaa aaacgtacgc aaatcgaaac
    58921 agaacatcaa aaacaaaaag aaaaacaaat cgaattactt gaaaaaaaat accgtcagct
    58981 taggaatcgc caacaagtag aagtacgaca agaaaatttg aatgcgaagc aggaattttt
    59041 acgacgattg tttgcagatg caatagctga aatggagaat tgggatgaat ctgaacaaat
    59101 ccagtttatc aaaaattcat tgtattcatt acctttgaca ggcaaagtaa cattcattgc
    59161 tggagaaaaa tcagcagctt atctttctca gacattgctg gatgaatgga acaatgaact
    59221 tccctttatg atggttttaa gtgatgaaac tgttgctgat caagcgggtt ttctgataaa
    59281 cgatcaagga gttcagtata attttctttt cagcagcctt gtacaagata tccaaggaac
    59341 gatgagtttt gagattgcca atcaattatt cgaataagga gggatcagtg tatggaatac
    59401 cacgaactaa atcccttgat tcgcggaaga gagcttgaac ttttgtccaa tgagacattt
    59461 gaacggatga ttcaaacaaa ttctgtcgaa gcacttggtg aaatattgaa atccacgatt
    59521 tacagtccat atatttatga cggatatgaa tatgacttcg aacagaatct tttaaaggag
    59581 cagagccgtc tatttcgctg gttaaaggaa tcagcaccgg agcctgaaat cgtttggatc
    59641 tatacgatgc gctatacttt ccataatctg aaggttttga caaaagccga gatgacaaat
    59701 caaaatctgg accatttgta tatcgacgat ggattttatt cattagaaac cttgaaggat
    59761 gccattcata cgcaagtttc aaatgaactc cctgaaagca ttatggacag tatacgagaa
    59821 gtacgtgatt attttgaaga gtctaccatt cttcagggaa ttgacgtgat ttatgatcga
    59881 cagtttttga cagagcagcg acggcttgcc gataagctag gatatcaaga attgctgaca
    59941 gaagtcattg cttttattga cttgaccaat atcacaacga tggctcgagg aatattgcag
    60001 aaaagaacat atgggttcat gacgacggta ctttccagct cgggaagtat cgaaaaagaa
    60061 gtatttttga actttgtaga taaagatatg gaagattaca cgaattttct attagagaca
    60121 gattatgctg attttctgcg ccctgtcatc cgtgagggaa atcttgatct cgtaaagctt
    60181 gaacagttga aagatgatta tctaagttca ttgtatcaag aagcacaaac ccaggctttc
    60241 ggaccactgc cactactagc ctttttgaac gcaaaagagg tagaaagcaa aaaccttcgt
    60301 ttactggtta tcggcaaaag aagccatttc gaaccagaaa caatcagaaa gcgggtgaga
    60361 caagtctatg acgcataaga tcggtgtagt tggcgacaaa gattctgttt tgccattcaa
    60421 attgtttggc tttgatgttc gttatggtac aaccaagcaa accgttcgtc aggcaattga
    60481 agaaatggca aagaatgagt atggcgtgat ttatgtcaca gagcaatgtg cagcattgag
    60541 cccagaaacg atcgaacgtt acaaggaaca aatgatacct gctatcgtat tgattcccaa
    60601 tcatcaagga tcattaggga tagggctgga agaaattcaa aaaaacgtag aaaaagcagt
    60661 aggtcaaaat atcttatagt atgaaaagga gatagaaatt ttgcaaattg gaaaaatcat
    60721 aaaagtctcc ggacctcttg taatggctga gaacatgtct gatgccagta tccaagacat
    60781 gtgtctagtg ggagatctag gagttatcgg agaaattatc gagatgcgtg gcgatgtggc
    60841 gtctattcaa gtatatgaag agacttctgg aattggtcca ggcgaaccgg ttcgctctac
    60901 tggtgaagct ttatctgttg aattaggacc agggatcatt tcacaaatgt ttgacggaat
    60961 ccaacggcca ttagatacct ttatggaaat cacacaaagt aatttcttag ggcgcggagt
    61021 tcaattgccc gcattggacc atgagaaaaa atggtggttc gaaccaacag tagaagccgg
    61081 agaaacggtg agtgctggag atgttatcgg gatcgtagaa gaaacaaaag ttataaaaca
    61141 taaaatcatg gtgccaaatg ggataaaagg aacgatcaaa acaatcgaaa aaggttcatt
    61201 cactattgat gaaacagtat gtgtcattga aaccgaaaat ggtgacaaaa atttgacgat
    61261 gcttcaaaaa tggccagtaa gacgtagtcg gccaatcaaa gaaaaattaa acccggatgc
    61321 acccatgatc actggacagc gagtgattga tacattcttc ccagtgacaa aaggaggagc
    61381 agcagctgta cctggtcctt ttggtgcagg gaaaacagtc gttcagcatc aaattgcaaa
    61441 atgggccgat gtggacatgg ttgtctatgt gggatgtggc gagcgtggaa atgagatgac
    61501 ggatgtttta aatgaattcc ctgaattgat tgaccctaat acaggagaat cgttgatgga
    61561 acgtacggta ttgattgcta atacatcaaa catgccagta gcagctcgag aagcttctat
    61621 ctatacggga atcacaattg cggaatattt ccgtgacatg ggttatgacg ttgctatcat
    61681 ggcagactcc acttcacgat gggcagaagc acttcgtgag atgagcggac gtttggaaga
    61741 aatgcctggt gatgaaggtt atccagctta tcttggttca cggcttgcgg aatactatga
    61801 acgatctgga agagtcattg cattaggatc agaaggtcgc gaaggaagta taaccgcaat
    61861 cagtgctgta tccccgtctg gaggggatat ttcggaacca gtcacacaaa atacattgcg
    61921 ggtcgtaaaa gtattctggg gtcttgattc cagtttagcc caaaaaagac attttccttc
    61981 gatcgattgg atacaaagct attctcttta ttcaacagaa gtagggaagt atatggatca
    62041 tatccttcaa gctgactggg catccatggt caccgagggc atgcgtattt tgcaggaaga
    62101 acagcagctg aatgaaatcg tgcgtttggt agggattgat tccctttcag ataatgaccg
    62161 tctgacatta gaagtggcaa aatccattcg tgaagattat ttgcagcaaa atgcttttga
    62221 tgatgtcgat acattcactt caagagagaa acaattcaaa atgttgaaga tgatcttgac
    62281 ctttggtgaa gaaggacgcc gtgcgttgtc gttaggtgct tatttaagtg agatcatgga
    62341 cggtaccgta tcgattcgtg aaaggatcgg acgaagcaaa tatatcccgg aaacagaatt
    62401 agaaaaacta gatcaaatta ttggtgaaat caagcaaacg atgcaaacga tcgtttcaga
    62461 aggaggcatg agcaatgatt aaagaatatc gtacgatcgg tgaagtcgtc ggtccattga
    62521 tggcggttga aaaagttgct ggtgtcaaat atgaagaatt aatcgaagtc cgtatgcaaa
    62581 atggcgagat tcgtcgtgga caagtattgg aagtccaaga agataaagca atggtccaaa
    62641 tattcgaagg aacaagtggt atcaatttac gtgactcttc tgtacgattt ttaggccatc
    62701 ctttagaact aggtgtatcg gaagacatga tcggtcgagt attcgatggt ttgggacgac
    62761 cgaaagataa cggaccagag atcctgcctg aaaagcattt ggatattaac ggagaagtaa
    62821 tcaatccagt agctcgggat tatccagatg agtttatcca aacaggtatc tctgcaatcg
    62881 atcatttgaa tacattagtt cgtgggcaaa aattaccagt attttctggc tctggtcttc
    62941 ctcataaaga attagctgct cagatcgcgc gacaagcaac ggtactcgat tcatcagatg
    63001 atttcgccgt agtctttgct gctattggaa tcacttttga agaagctgaa tttttcatgg
    63061 aagattttcg tcaaacgggc gcaatcgacc gttctgtcat gtttatgaat ttagcaaacg
    63121 accctgcaat cgaacgtatc gctactcctc gtatggcttt gactgctgca gaatatcttg
    63181 cttatgaaaa gggaatgcat gttttggtta tcatgacaga catgaccaac tatgcagagg
    63241 cattgcgaga gatttctgca gcacggcgag aagttccagg aagaagagga tatcctgggt
    63301 atctttacac gaacttagct actttattcg aacgagcagg aagaatccgc ggactaaaag
    63361 gatcggttac tcaaattccg attctaacaa tgccagaaga cgataaaact catccaatcc
    63421 ctgacttgac tggttatatt acagaaggac agatcatctt gactagagag ctttacaaga
    63481 gtgggattca gccaccaatt gatgtactcc cgtctctttc gcggttaaaa gataagggaa
    63541 ctggacccgg aaagacaaga gaagaccatg cagctacgat gaatcagctt tttgcagcct
    63601 atgcacaagg aaaacaggca aaagaattag ccgtagtttt aggtgaatct gctttatctg
    63661 atgtagacaa gatctatgcg aagtttgctg agcggtttga aaaagaatat gtcaatcaag
    63721 gattctacac gaaccgttcg attactgaaa cacttgatct agggtgggaa ctgctatcga
    63781 tgttgccaag aaccgaatta aaacgaatca aagacgacat gctggacaaa tatctgccag
    63841 aagggaagtg agttctgatg gcacgattga atgtgaatcc gacccgcatg gaattgactc
    63901 gattgaaaaa acagttgaca actgccacaa gagggcataa attattaaaa gataagcaag
    63961 atgaactgat gcgtcaattc atcctgttga ttcgaaagaa caaccagctg cgtcaagaaa
    64021 tggaagagtc tatgacagaa gcaatgtctg attttgtatt ggcaaatgct tcgatcacgg
    64081 aacctttcat cgaagaatta tttgttttgc cggcggaaaa cgttgatttg tctatcgtag
    64141 acaaaaatat catgagtgta aaagtacctg tgatgaattt tgaatatgat gaacagctga
    64201 cagacacacc tttggaatac gggtatttga attccagtgc cgaactggat cggtcaatcg
    64261 atcggtttac taccctgctt ccaaaacttt tggaactgac agaaatcgaa aaaacttgtc
    64321 aactgatggc gagtgaaatc gaaaagacac gtcggcgagt caatgcatta gaatatatga
    64381 cgatccctca attagaagaa acgatctatt atattcgtat gaagctggaa gaaaacgagc
    64441 gcgcagaagt aacaaggttg atcaaagtaa aaaatatggg taccactgac taagtggtat
    64501 ctttttttaa aagaggaaaa cgatgattaa acaaagcaaa caaataaaga aacgtttgcc
    64561 ggctgttcag cttctcgcat taggattttt tctcctgatt ttagttggcg gcggcctact
    64621 taccctacca ttttttagta atagtgggca tgggacaaat tttatcgatg cgcttttcac
    64681 tgcaacttct gctgtctgtg taacgggatt gacaacattg aatacagcag agcattggaa
    64741 cagtgctggt caatttttga tcatgctttt gattgagatc ggcggtcttg gttttatgat
    64801 ggttccgatt ctatgctttg cattgataaa gaagaaagtc agtctcagca cacgtattat
    64861 attaaaagaa gcgctgaatc tagaagaagt atcaggtgtg atgaagctca tgctttacat
    64921 cctaaaattg gcctttgtta tccaattgat tggtacggtt gctttatctt ttgtatttgt
    64981 gcctgaattt ggctggttga aaggtatctg gtacagtttg ttccatgcaa tctctagttt
    65041 ttgtaatgca gggtttgatt tgttaggaga cagtttagcc aatcaccagc aaaatgttta
    65101 tttgatcatg gtggtatctg ctttgatcat ttctggtggg ttaggcttca tcgtatggcg
    65161 tgatttactg gaatatcatc agaaaaagaa gatgacgatt cattcaaaaa tcgcattaac
    65221 cgttacaggt acccttttat taggcggatt cgttgtgttc tttttcacag agcgcaatgg
    65281 ggcaacgcta gtggaaggga atatgtttca acgattggca aacacctttt ttatgagtgt
    65341 gacacctaga acggctggat attattcagt tgattacctc catatgagcc atgcaggtct
    65401 gattttaacg atgtttttga tgtacatcgg cggaacatct gggtcgacgg ccggcggatt
    65461 gaaaacaact acgttaggtg tgttgctgat ccagatgcgt tcgatgttga aaggacgtac
    65521 gagagcggaa gcatttggcc gtaccatccg acaaggcgca gtgttgcggg cacttacttt
    65581 attttttgtt acgttgacgt tgtgtgtagt cgccatcatg attttatcgg tgactgaaac
    65641 gatacctaaa acttctggca tcgaatatat tgcttttgag gtcttctctg cgtttggtac
    65701 agtaggtctt acgatgggat tgacgccaga cttgactgca atagggaaat tgatcattat
    65761 agcgcttatg tacattggac gagttggtgt attgactgtg gtcttctctc tgatgacaaa
    65821 agcacaaaga acacaatcaa aatacaaata tccagaagaa agtgtcatga ttggataatt
    65881 tcatcttctg acagaaacct agtgatttag taaaataaga aaataaaata atttttcttg
    65941 cttcttggat actttaagtg caaaatgttc gtttattttt tgaggcacta cccgttgttg
    66001 aacctcgggt agtgcctctt ttttattgaa aggaatcaat aagatttgat tcctttattt
    66061 atcaataggt tataattcat atttttttat tttttctttt acgatactta gtccagaagc
    66121 taaaatttca aggttattag aggataggga aattcgaacg aatgcatctg gttgcgcacg
    66181 tgtagtaaag cgtctggaat gataaagtcg gataccgtta gcaagaaagt cattctcaat
    66241 tatcttaggg tcaatctcag gatcaactgg aagaattcga tagtaaggga cacgattcgt
    66301 tggtttttct actttgaaaa gagaatcaaa aagctgattg gctctttttg taagacttaa
    66361 tttagtttct aagatcctat ttgcttcatt tgaatggagg atttgagtga tgacttccac
    66421 atctaatcca gaagttttca catttatatt aaaaattgct tgttctatct tgctttggac
    66481 atgtactgga actacaagat aagcaactcg caatccagaa caaataaatt ttgtcattcc
    66541 agctaagtag attgtttggt taggtaataa attttggaaa ggaggaatga tcgtatgttg
    66601 atagtaagtt gttaaaaatg aatggatatc atcttcaata acccatagac cttctttttc
    66661 aatcacttcg gctaattgct ctcgtctttt gtgagagatt tgaaacccta taggattatt
    66721 acaagaaggc ataagaaaga caccatgaat cttctttttt ctacattcct gaatcaatat
    66781 atcaggtcgc attccttgct cgtcaaaatc aattggaacg atctctaaat gatataactg
    66841 agccaattca atgaaattag cgtatgtata tcggtctgtt gctattctat cacctggaga
    66901 gaaaagtgca gaaagtgtga tagccaatcc attttgaaca ccggaaacaa tagatagaga
    66961 ttctaaggac ggaatgactc cttgagtttt taaccaattt gctgcagtag tcaattgaaa
    67021 atttgtgcct ttgggatctt ggtaattaag caaaccaaca agatttgggt tattactagt
    67081 tgattgaata aacgggacaa cgaatttatt acattcttca aaagaactaa ccagaccaaa
    67141 atcaatgata tgttcggtta cttcattggt agagatggtg atggactcaa agacattggg
    67201 agaaataaat gtgccacttc caatattcgt atacagcaaa cctttctcaa ttccatattt
    67261 ataagccttg ccaacggtag taaaatttaa atctaaataa tcagctagct ctctttggga
    67321 gggtaactga gtgtttttct gtaatttatt ctttaaaata tcttgttcta attgttgaat
    67381 caacgatcga taataaggac gcttcaattt ttgtttgtct ggtcgccaag ttaacgggta
    67441 attgtcataa gaattaatag gcattttctc cactccttaa aattgtatac catacaattt
    67501 taccattgat tgtattgatt ttagaagtta atcttttgtg aggagggaat tgtctatgaa
    67561 aattattaat tttgaggaag tagtagcttc ttttagtttt gaagaagcga ttcaagtaat
    67621 gaaaaaatgt ttttttgatt atgaaagtgg taaaatcagc caaaatcctc gattggtgga
    67681 aattttacct gacggaaaag gcaaaaatat gtttgcgatg atgcctgctt atttggggga
    67741 gaacagatat tttggcacaa aagtcattac cgcttttccg gataatcatc agtatcattt
    67801 accttctcat ttaggagaga ttttcttgtt tgatgctcaa aacggactac ccaaagcatt
    67861 gataaacgca aatgcagtca cctggattcg aacagctgct gtatctgcgc tggcaacaga
    67921 ttacttagca aatccgcatg cagaaacact tgctttaatt ggagctggtc aacaagcatt
    67981 gtcgcatttg actgcgattt ctaccattcg tcccatcact acagtgtttg tttacgatat
    68041 tttagaagaa agacgaaata cttttatcgc taaagctaaa aaacagtatc ctaaaataac
    68101 gtttataaat gcaatatcag tagaagatgc agtacgtcca gcggaaatca tttgtacgtt
    68161 gacctctagt aaagaggcat ttcttcaaaa agagtgggta agggaagacg cacatatcaa
    68221 tgctgtgggc acatttactc ctgatacaag agagataagt acatcgttga tggaaacttc
    68281 tcaaatctat gtagatgatt actcagctgc cttaacagaa agcggggacc tgcttatagc
    68341 catggcagaa ggagatttag aggaatcttg tattgttggt acattaggcg aattagtttc
    68401 tgagaaaaaa acattttata aaggagaatc gaggccgacg attttcgacg cagttggtct
    68461 agctattgaa gatctttgtt gtgcagaata tatttataac aaattagaga aaggggcttt
    68521 aaaatgaact tagattatta cgtagttgat gcatttgcag atgaggtgtt caaaggaaat
    68581 ccagcagcgg tttatgtgct agaagaatgg ctgccagaag gtacgatgca aaaaattgcc
    68641 attgaaaaca atttatctga aacagcattt acagtgaaga aaaaccaaga atttgagctt
    68701 agatggttta cgccagatcg agaaatcgat ctttgtggtc acgcgacatt agcaacagca
    68761 tttgttttat ttaactatta caaaatacct gatgaaacga ttaaattttc tactcaaagc
    68821 ggtaatctat ttgtgacaag aattaaagat tattattaca tggattttcc tagtattatg
    68881 ccgaaaaaag tgccgatatt gtctgaatat gaagaagcga ttggggctaa gattaaagaa
    68941 gcctacttag cacgtgattt atttttcgta ttagaagatg aaaaaacggt ggctaaattg
    69001 cagccagatt ttacagctat caaaaatttt gaattaggaa taggagtcat cgtgacagca
    69061 gaaagtgtgc aaaaagactt tgtttctaga accttctttc caaaattaac aatcaatgaa
    69121 gatccagtat gtggatcagc acattctaat cttattcctt attgggcaga aagaatgaat
    69181 aaaacaaaat tgtcagctta tcaaatgtca tcaaggggcg gggatctaga atgtgaatta
    69241 aaagaagatc gtgtgataat aggaggaaaa gcagttttgt tttctaaagg tacctcgttt
    69301 atttgatttc tttcaaatga attaaaaata aattggtgga gagtggcaac cttatagcta
    69361 caatggttgt taaaaaaaga cgcaaacttt ttcaatttaa tgaaaagttt gcgtctcatt
    69421 ttaattattg gtgtttgttt tttattcaat caagagtaaa atgccccata ctaactatcc
    69481 tcggatgact tcgattttat aaccatcagg gtcaatgaca aagtagtaag aaggcgcagt
    69541 gccaggtaat ccttttaagt cagtaatctc aagaccggca gctttatgtt gctcgtgtaa
    69601 tttttctaga tcatcagctg ctatagcgat atgaccatag ccgtctccta gatcatacgc
    69661 accatgatca tagttgtaag tcaactctaa ttcatagtca tccccaggaa gtgtcaaata
    69721 aacaagtgta aatttgtttt ctggaaaatc acggcggcgg ctttcttcaa aaccaaatgc
    69781 cttttgataa aattcgattg atgcttcaag atctttcact cgaacacatg tgtgagccat
    69841 tttcatagat tcatctctcc ttttcctttt actcaatcat aacaaaaaga agtaaggaaa
    69901 tagttgattt gtgcttgttc atgagaaatt tctcatgtaa aatggataga aaataaaact
    69961 gttaaggtgg aaactattca tggataaacc aatttttggt gaaaaaaagc aatttgctga
    70021 ttatcaaacc cgttatgcag catatattgt catagaaaga gatcagcaga ttgcagtaat
    70081 cgaggctcca aatggtgctt tttttcttcc tggcggggaa attgaaggag aagagacgaa
    70141 agaagaagcg attgtccgtg agctgataga agaaatggga attgtggcag aaatttcttc
    70201 ttatctggga gaagctgatg agtattttta ctcgaattat cgtgcaactt attattataa
    70261 tccaggatat ttctttgttg ctgattcttg gaagcgtata ggagaaccaa ctgaaaagac
    70321 caataaaatt tggtgggtga gccctcaaga agcgcttgtg aaattgaaac gtggtagcca
    70381 ccgatgggca gtcgagagat ggatcgaaaa aaataaaaac tgaaagaaag cgagtagatc
    70441 agctttctct cagttttttt atttattaca gtaca
//
LOCUS       DACKAC010000006        61764 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.006, whole genome shotgun sequence.
ACCESSION   DACKAC010000006 DACKAC010000000
VERSION     DACKAC010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61764
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.006"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            6..305
                     /locus_tag="I0B46_000409"
     CDS             6..305
                     /locus_tag="I0B46_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAR2082738.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="I0B46_000410"
     CDS             378..1739
                     /locus_tag="I0B46_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAR2082739.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="I0B46_000411"
     CDS             1761..2021
                     /locus_tag="I0B46_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2082740.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="I0B46_000412"
     CDS             2035..2787
                     /locus_tag="I0B46_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAR2082741.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="I0B46_000413"
     CDS             complement(2881..3486)
                     /locus_tag="I0B46_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAR2082742.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="I0B46_000414"
     CDS             complement(3539..4513)
                     /locus_tag="I0B46_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAR2082743.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5670)
                     /locus_tag="I0B46_000415"
     CDS             complement(4891..5670)
                     /locus_tag="I0B46_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAR2082744.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="I0B46_000416"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="I0B46_000416"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAR2082745.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="I0B46_000417"
     CDS             7161..8558
                     /locus_tag="I0B46_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAR2082746.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="I0B46_000418"
     CDS             8639..9079
                     /locus_tag="I0B46_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAR2082747.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="I0B46_000419"
     CDS             9227..9553
                     /locus_tag="I0B46_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAR2082748.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="I0B46_000420"
     CDS             9554..10303
                     /locus_tag="I0B46_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2082749.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="I0B46_000421"
     CDS             complement(10383..10838)
                     /locus_tag="I0B46_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAR2082750.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="I0B46_000422"
     CDS             complement(11265..11540)
                     /locus_tag="I0B46_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2082751.1"
                     /translation="MKLEDKKVILKKIKRLPEQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="I0B46_000423"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="I0B46_000423"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAR2082752.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(12978..14429)
                     /gene="cls"
                     /locus_tag="I0B46_000424"
     CDS             complement(12978..14429)
                     /gene="cls"
                     /locus_tag="I0B46_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAR2082753.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            14770..17436
                     /locus_tag="I0B46_000425"
     CDS             14770..17436
                     /locus_tag="I0B46_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAR2082754.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            17800..18552
                     /locus_tag="I0B46_000426"
     CDS             17800..18552
                     /locus_tag="I0B46_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAR2082755.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(18574..19728)
                     /locus_tag="I0B46_000427"
     CDS             complement(18574..19728)
                     /locus_tag="I0B46_000427"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAR2082756.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            19913..22354
                     /locus_tag="I0B46_000428"
     CDS             19913..22354
                     /locus_tag="I0B46_000428"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAR2082757.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(22684..23388)
                     /locus_tag="I0B46_000429"
     CDS             complement(22684..23388)
                     /locus_tag="I0B46_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2082758.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(23575..23820)
                     /locus_tag="I0B46_000430"
     CDS             complement(23575..23820)
                     /locus_tag="I0B46_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2082759.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(23837..24688)
                     /locus_tag="I0B46_000431"
     CDS             complement(23837..24688)
                     /locus_tag="I0B46_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAR2082760.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            24854..25858
                     /locus_tag="I0B46_000432"
     CDS             24854..25858
                     /locus_tag="I0B46_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAR2082761.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(26099..26455)
                     /locus_tag="I0B46_000433"
     CDS             complement(26099..26455)
                     /locus_tag="I0B46_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAR2082762.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            26823..28010
                     /gene="tuf"
                     /locus_tag="I0B46_000434"
     CDS             26823..28010
                     /gene="tuf"
                     /locus_tag="I0B46_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAR2082763.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(28198..30789)
                     /gene="mprF"
                     /locus_tag="I0B46_000435"
     CDS             complement(28198..30789)
                     /gene="mprF"
                     /locus_tag="I0B46_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAR2082764.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(30815..31534)
                     /locus_tag="I0B46_000436"
     CDS             complement(30815..31534)
                     /locus_tag="I0B46_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAR2082765.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            31743..32375
                     /locus_tag="I0B46_000437"
     CDS             31743..32375
                     /locus_tag="I0B46_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAR2082766.1"
                     /translation="MKRKRLSIIRILFYMIILTVIGAVLFLGGRTYRSVKRVESYQIE
                     ITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSESLHGEAEIIDNPADSIDQGVK
                     YLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHGGENTVELAEAYSKDVLSPLLG
                     NEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKMNEKIIETYEKVFK"
     gene            32434..33294
                     /locus_tag="I0B46_000438"
     CDS             32434..33294
                     /locus_tag="I0B46_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAR2082767.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            33401..35794
                     /locus_tag="I0B46_000439"
     CDS             33401..35794
                     /locus_tag="I0B46_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAR2082768.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            35976..37817
                     /locus_tag="I0B46_000440"
     CDS             35976..37817
                     /locus_tag="I0B46_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAR2082769.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            37841..38950
                     /gene="rpoD"
                     /locus_tag="I0B46_000441"
     CDS             37841..38950
                     /gene="rpoD"
                     /locus_tag="I0B46_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAR2082770.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            39170..41269
                     /locus_tag="I0B46_000442"
     CDS             39170..41269
                     /locus_tag="I0B46_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAR2082771.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            41465..42328
                     /locus_tag="I0B46_000443"
     CDS             41465..42328
                     /locus_tag="I0B46_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAR2082772.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            42433..42906
                     /locus_tag="I0B46_000444"
     CDS             42433..42906
                     /locus_tag="I0B46_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAR2082773.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            43002..43889
                     /gene="xerD"
                     /locus_tag="I0B46_000445"
     CDS             43002..43889
                     /gene="xerD"
                     /locus_tag="I0B46_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAR2082774.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            43908..44279
                     /locus_tag="I0B46_000446"
     CDS             43908..44279
                     /locus_tag="I0B46_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAR2082775.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            44249..45046
                     /locus_tag="I0B46_000447"
     CDS             44249..45046
                     /locus_tag="I0B46_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAR2082776.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            45030..45599
                     /gene="scpB"
                     /locus_tag="I0B46_000448"
     CDS             45030..45599
                     /gene="scpB"
                     /locus_tag="I0B46_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAR2082777.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            45605..46321
                     /locus_tag="I0B46_000449"
     CDS             45605..46321
                     /locus_tag="I0B46_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAR2082778.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     regulatory      46403..46528
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            46651..47262
                     /locus_tag="I0B46_000450"
     CDS             46651..47262
                     /locus_tag="I0B46_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAR2082779.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     gene            47640..49163
                     /locus_tag="I0B46_000451"
     CDS             47640..49163
                     /locus_tag="I0B46_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAR2082780.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            49400..50740
                     /locus_tag="I0B46_000452"
     CDS             49400..50740
                     /locus_tag="I0B46_000452"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAR2082781.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            50893..51630
                     /locus_tag="I0B46_000453"
     CDS             50893..51630
                     /locus_tag="I0B46_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAR2082782.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(51795..52016)
                     /locus_tag="I0B46_000454"
     CDS             complement(51795..52016)
                     /locus_tag="I0B46_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAR2082783.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            52047..53078
                     /locus_tag="I0B46_000455"
     CDS             52047..53078
                     /locus_tag="I0B46_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2082784.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            53065..54468
                     /locus_tag="I0B46_000456"
     CDS             53065..54468
                     /locus_tag="I0B46_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAR2082785.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            54534..55169
                     /locus_tag="I0B46_000457"
     CDS             54534..55169
                     /locus_tag="I0B46_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAR2082786.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            55218..55898
                     /locus_tag="I0B46_000458"
     CDS             55218..55898
                     /locus_tag="I0B46_000458"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAR2082787.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTREVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            56033..57262
                     /gene="rpsA"
                     /locus_tag="I0B46_000459"
     CDS             56033..57262
                     /gene="rpsA"
                     /locus_tag="I0B46_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAR2082788.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            57444..58754
                     /locus_tag="I0B46_000460"
     CDS             57444..58754
                     /locus_tag="I0B46_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAR2082789.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            58997..59272
                     /locus_tag="I0B46_000461"
     CDS             58997..59272
                     /locus_tag="I0B46_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAR2082790.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            59507..60409
                     /locus_tag="I0B46_000462"
                     /pseudo
     CDS             59507..60409
                     /locus_tag="I0B46_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            60516..61595
                     /locus_tag="I0B46_000463"
     CDS             60516..61595
                     /locus_tag="I0B46_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAR2082791.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
BASE COUNT        20277 a        10844 c        13015 g        17628 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttgctct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    12721 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    12781 aggtcgtgag cctgccgtcc agctccgagt cacaaggaac aagttcaaaa aacagcttct
    12841 catgtttgtt gaatattgtg acttgtgacc gaggagttgg caggagaaca ccgtttgttc
    12901 ggaagaaggg actgtgacaa gatttttgtc acagtccctt taatcttagt atatttttct
    12961 ctacttgaat aaaaaaatta taggattgga gaaaataatc gagaaatttg ttgcttgaat
    13021 aatagccagt tagacatttc cttgactgtt tcagttgtca tggatgaaca atgtgtctca
    13081 tcttctttga actgagtagc aagttcttct aaaaactcag gatcgtaaat gacagcactt
    13141 gtttcaaaat tcaatcggta gctacgaata tcttgatttg ctgaacctac catgcagatt
    13201 tcatcatcca ttatcaatgt ttttgcatgg aggaagccgt tctcatagac aaggatttgt
    13261 acattttcct tgatcagctg ccgagcataa tattgtgttg ctcgataaat aaaaggatga
    13321 tccggtttgt tgggaatcat gattttcaca tctacaccag aggcagttgc gatttttaaa
    13381 gcagcaatga cactttcatc aggaacaagg taaggtgtct gtatccaaac acgtttttta
    13441 gcagaagtaa tcaatttgat aaatgaaagc ttgatttgtt ccctttcgtt attcggtccg
    13501 cttgaaacaa gctggatgga tgtatcagca agcttagatt catctcgatc tgctttttta
    13561 aagaaataat tcaaatgata gccgaccttt ttttcttcag gtaccgagac gttccagtct
    13621 gttaagaagc gaagctggag caaagaagaa gcggcaccga aaatccgtat atgcgtatcg
    13681 cgccaatagc cgaatttttt tgttgtattt acatattgat tggcaatatt gaaaccaccg
    13741 gtataactaa tctttccatc gatcacaacg atttttcggt gatcatggta attcaatcgg
    13801 aaacgaagta atgccctttg tgaggtaaca aatgtatgga catgtccacc atttttgatt
    13861 agacgattga aatcttttgc ttttgtgcca tgagagccaa atgcatcata taatattcga
    13921 acttccacgc cttctgcggc tttttcttct aaaacatgta agacttgctg gccgatatta
    13981 tctgtcacaa atgcgtaata ttcgatatgg atcgaatgtt tggctttttt tagatcttga
    14041 agcagtgaat ccaatttctc ttgtccgtct gtgagaagag tgacagaatt cattcttgtc
    14101 agcggcatac gatttaatga agagaagaag tcaacaaatt gctgtttgtc tttatcgccc
    14161 atgtcaggat cgtaatgttc aatggtatct cctctaaaag actgaaagtt ttctaattct
    14221 ttcaagtcac tttgtcggag atagaatttt ttcttatccg ttaatccacg tccgaaaaat
    14281 aaatataata caaagcccac cccaggaagg gcaaacaata ctaagagcca tgcccaaatg
    14341 gctgctacat ctcggggttt gatcaaaata gccaccaaag caataagtgc atttaataga
    14401 taaagggcgg ttataatact agataccaca aaaatgttcc tccttacata caataacatt
    14461 agtatagcat gatagaagaa agaagcctac caaggtgaat atacttttca ctcctttcat
    14521 gaaaatgtag gtaaatcggg ctaaagtagt tgctttttgt aggttaataa ggtaggatat
    14581 tagtacagtt gaatatctcc ttaggggagt aaccggcaac gtttgttgcg ataatatcaa
    14641 cagtaagtta accaaatggt tttctggtat tgtatgaaat ggtgagacct aagacaaatg
    14701 cgtatttgtc ttaggtctct tttttttcag acaggactag ggccaagatc aaaaaatggg
    14761 ggatgaaatt tggaaaacaa ggaacaagca caacaagcgg tctataaaga aacgatcgac
    14821 tcgttgatgc agcaaacgca gagctcaacc gatggattga gcaccacgca agcgaaggaa
    14881 aaattagaaa gagatggctt gaaccagcta aaagaaaagc cgaagaagcc aacttggaaa
    14941 ttgttcttag aaactttcaa agatgcaatg gttatcgtat tgctgattgt cgcactgatt
    15001 cagatggtca tgggtgacta catcgaattc ttagttattt ttgcagtatt gatgttgaat
    15061 tctataatca gtgttgtcca aacaaaaaaa gcagaaggtt ctttagaagc gttgaaaaac
    15121 ctttcagcgc ccgatgctcg agtgattcgt gatggtaagg aaaaaacgat tccggcaaac
    15181 gaacttgtgg taggagatat cgtgttgtta gacgcaggcg attatgtacc tgctgatgga
    15241 cgtttactag atgcgggatc attaaaagta gatgaaggta tgctgacagg agagtcgact
    15301 tctgcagaaa aagaagtaac ggatatcaca agtacggtac caatcggaga tcgtacaaat
    15361 atggtattca gtggaacgat cgtaacttac ggacgcggta aattcttagt gacttcaact
    15421 gggaataata cggagatcgg acaagttgct ggccttttgg aatcaacaac tgagcaagtc
    15481 actccgttac aacgaggtct ggataaattc agtaaaaaac taagtttggc tattttggca
    15541 ttgtctttat tgattttagg tatccaactg tttcgtattt atcttggaga aggtacagga
    15601 gatatgactg cagatatcgt cagcgcagtg atgtttgccg tagccgtagc cgtagctgct
    15661 attccagaag cattacagtc tatcgtaaca atcgtgttat ctttgggaac gaataaaatg
    15721 gcaaaacgtc atgccattat tcggaagctt ccagcagttg aaacacttgg ttcgacaagt
    15781 atcatctgta cagataagac tggtacattg acacaaaaca aaatgacagt tgttgactac
    15841 tttttaggta atggcgatac tggggatttc acaaatgacc cttcaaaatg gacagctgac
    15901 gaacgtcgct tgatcgaaat cagtgtttta gcgaatgatg catcgatcag tgaagacggt
    15961 actaaattgg gcgaccctac ggaagtagcg tttatcgact ttagtgaaaa attaaaccag
    16021 ccttatcagg aaatccgaaa caactaccca agacaagcag aacttccttt tgactcagat
    16081 cgaaaattaa tgtctactgg acacacaatt gataacaaaa attacttgtt tgttaaaggc
    16141 ggaccagata tcgtcttcca acgcagtaaa aaagtattgc taaatggtga agtcgttccg
    16201 atgacggatg aattgatgaa aaaattccaa gagcaaaatg aagctttttc aacacgcgca
    16261 ttacgtgtac tagcttttgc ttaccgtcca cttgataatt ttgatttatc atttgaagat
    16321 gaagacgatt tgatccttgt tggtttgctt gctatgatcg atcctccacg tgctgaagtg
    16381 actcaggcgg taacagaagc aagaagtgca ggaatcaaaa caatcatgat tactggtgac
    16441 cacaaaacaa cagctgttgc tatcgcaaaa caaatcggga tttttgaaga aggaaacctg
    16501 gctttgaccg ggacggaact agatgaattg acagaagatc aactaaacga agttttagaa
    16561 aaagtcactg tttatgcacg tgtttcacct gagaataaaa ttcgtatcgt tcgtgcttgg
    16621 caaaataaag gtcatatctc agctatgaca ggtgatggtg taaatgacgc accagcattg
    16681 aaacaagcag acatcggaat tgctatggga acgggtacag atgtggcaaa agatgcagca
    16741 gcgatggtat tgacggatga taactttgct tctattgtca gcgcagtaga agtaggacgt
    16801 aatgtgtttg ataatatcaa gaaagctatt tcttatctat ttgcaggaaa tttaggtgcg
    16861 atcattgcga tcatctttgc cttagttgct gactggtcca atccatttac agcattacaa
    16921 ctgttgttca tcaatttagt caatgattca ctgccagcaa ttgcattagg tacagaaaaa
    16981 gctgaacctg ctacgatgaa ccgcccgcca agagatccaa attcaggaat ctttacggac
    17041 aaaacattga tttctgtaac gtatcgtggt gtgctgattg ggattgctgt tatctttgct
    17101 caatggcttg gcatgcagca ttcacctgaa ttcggtgtag ccatgtcatt ttcaacattg
    17161 atttggtgtc ggacattgca aacattccca gcacgttcaa acacacaaac agctatccaa
    17221 gcagggttct tctctaacag aagtgtctta ctagctgtag ttgtatgtag cgcgctttac
    17281 tgcttaacaa tgatcccagg actgaaagaa gtcttctcta tcccatcagc atttggattg
    17341 acagaagtct ttacatgtat gggaatcgca cttgtgacaa ttgttttgat ggaaatcact
    17401 aaattgatca ttgtacgagt acaaggaaat aaataaaacg aatcaacgag gttgtgagcc
    17461 tgccgtttaa ttctgagtca caaggtgcaa tttcaacaaa cagcttctca tgtttgttga
    17521 atattgtgac ttgtgaccga agagttggca ggagaacacc gtttaaggaa gaagttgtaa
    17581 aatcagcatt ttgagctaat actgagggtc atctgattgg acactgttta ttcggaaaga
    17641 gggactttga cacgactttg gtcacagtcc ctttttttat ttatgataga cggaaagcca
    17701 atcattttat tattcatcaa aagagataga atagataaaa gaggagaatg atttattccg
    17761 aaaagtgaaa acatataaca aaaaattaga ggaaatcaaa tgaatgaaaa agaatgggat
    17821 gaatttgctg aagaatatta tgaaatccag caagaatcgc aagtaactat tgctgaagat
    17881 gtaaaaaatt atctgaaaac ggagaaagtc ctgcctgctg aaacagcagc agacgttgct
    17941 ggaggatctg gcagatttct ttccttagca aaagaaatta atcgctatga tttgatcgat
    18001 ttttcttcag gaatgctaga aaatgcaaaa aaagaagcag aaaaaaatga gttgccaaat
    18061 gtttccttta tcaaacagtc gttagaagaa tttatccaat caaatgagac atatgaaatg
    18121 gtttttacag cagcaaatcc agcattggta catcttattc aattagaaaa attgatgaaa
    18181 aagtcaagca aatggtgcgt gataatcaga gtagtggaat caatcgatga tgttttttat
    18241 cctatcgaaa agcaactagg aatgatagaa gaagatccta atacatcgcc agttgtaatg
    18301 gatcaatttg aaacatactt aattgatgaa ggattcaaag tgaagaaaaa agattttact
    18361 tattttacac aggaatcaat tactgaagaa ttgatgaagc tatactatga cgcgtacgca
    18421 aaagatcctg ttgtgaccag gtacattcgc aaactattca gtgaaaaacg agaaatcctt
    18481 agtacgacta agctgaccta tagactgctc ctttggtccg cagataaaaa aagaaccttt
    18541 cacgactcgt gaagggttct ttttatgatt ttattatatt ttgtagtaac gaatatcatt
    18601 ttcgatcttt gtaatcgaaa aaggtacatc tgaagtatat tctgcacatt ctccgtgttc
    18661 aggaagcgtc tcactgaaaa tcgtttcata ttcgtcaaca gtgatccgtg ttcgagaatc
    18721 tagcatctct tgatgagatt gtgcgaagag atattcttga tagttttctt ccagataacc
    18781 tgtaaaaaat tcacttactg caccagaacc gtagctgaat agaccgatgc gatctcctgg
    18841 ctgtagtgat ttcgaatttt ccagtagaga agttagcccc aggtataatg aaccagtgta
    18901 aagattaccg attcgtcggc tgtaacggat gctttcttca tagcgagcca taagacgttc
    18961 ctgattttct tcgtctgttt ggtctaatac gctttgcaat gccttttttc ccatcttagt
    19021 atacggaata tggaaagcaa tcgcctgata atcttcgagt cctcgacccg acaattcttt
    19081 atgtcgattc caaacttttt ggaatgattc gatatacgta gaattagata aaggaccatc
    19141 aacaacagga aattcgctat aatctggacg ccagaaatca tagatatctt ctgtcagaaa
    19201 tacgctgtcg tcttcaatcg ataaaatacg cgggttttga gtgatcatca tcgcaacagc
    19261 accgacacct tgcgtcactt caccaccgct tgccaagccg taacgagcaa tatcacttgc
    19321 tatgactagt acttttcgtt ctggatgatt tttgacatat tctttcgcca tgtgcaaagc
    19381 tgcggtgcca ccgtagcaag cctcttttac ttcaaaagaa cgagcaaaag attggatttt
    19441 cagtaaatgg tggataatta ccgcactcgc ttttgaatga tcaatcccgg attctgttcc
    19501 gacgatgacc atgtcgatta gctcacggtc tttttctgtt acgattttgc tggcagcatt
    19561 tgctcctagt gtaatgatgt cttcatttgc acgattgact gccatttggt cttgaccaat
    19621 ccctatatgg tattttgcag gatcatcccc acggctttct gccaactctg tcatatctaa
    19681 atataaatta ggaataaaaa aggaaagacg atcaatccct attttcattt caacacctct
    19741 ttacttatga taaaaaactt actttactaa ggtatcataa aaagattaaa agaaatataa
    19801 aaaagagaat atttaaggat tgttaaaatt taatcttaga aaaaaataac tggaacaaga
    19861 gctatttgtt tttacagcaa agaatggtat atttttgtag gaggtggctt acttgaaaga
    19921 agtcgttatg atagatgctg caagaacgcc gataggaaag tatcgcggaa gtttaagtcc
    19981 atttactgca gttgaattag gcacacttgt gactaaagga ttattagata aaacaaaact
    20041 gaaaaaagat aaaatcgatc aagtaatatt tggaaatgtc ctgcaagctg gtaatggtca
    20101 aaatgttgcc agacagattg ctttgaacag cggtctccct gtagatgtcc cagcaatgac
    20161 gatcaatgaa gtttgcggat ctggtatgaa agcagtgatc ttagcacgcc agctgatcca
    20221 attaggtgaa gcagaacttg tgatagccgg cggaacagag agtatgtctc aagcacctat
    20281 gctgaaacag tatcagtcag aaacaaatga atatggtgaa ccaatttcca gtatggtcaa
    20341 cgacggattg actgacgcat tttcaaatgc acatatggga ttaaccgcag agaatgttgc
    20401 aacacaattt tctgtgagca gagaagagca ggatcgctat gccttgtcgt cccagttgaa
    20461 agcagcacat gctgtcgaag ccggtgtatt ttctgaggag atcatcccag tcaagatttc
    20521 tgatgaagac gtgttatctg aggatgaagc agttcgtgga aatagtacat tggaaaaact
    20581 gggcacgtta cgtacagtat tctcagaaga aggaactgta acagcaggaa atgcttcccc
    20641 gttgaatgac ggtgcctctg tggtgatcct tgcatccaaa gaatacgcag aaaataataa
    20701 tctgccttat ttagcaacca tcaaagaagt agcggaggtc ggtattgacc caagtatcat
    20761 gggaatcgct ccaatcaaag ccattcaaaa attgacagat agatcaggaa tgaatttatc
    20821 aacaatcgat ctttttgaga tcaatgaagc atttgcagct tcttctatcg tagttagcca
    20881 agaactgcag ttagatgaag aaaaggtcaa tatatatgga ggagcaatcg cattaggcca
    20941 tccaattggt gcaagcggtg cacgtatctt gactacatta gcttacggat tgcttcgtga
    21001 acagaagaga tacggtattg cttcattatg tattggtgga ggtttaggct tggcagtctt
    21061 gttagaagcg aatatggagc aaacacataa ggatgtgcaa aaaaaaaagt tttatcagct
    21121 tacgccatct gaacggcgca gtcaattaat agaaaaaaat gtattgacac aagaaactgc
    21181 gttgattttt caggaacaaa cactttctga agagctctcc gatcacatga tcgaaaacca
    21241 agtgagtgaa gtggaaatcc caatggggat cgcgcagaat tttcaaatca atggcaagaa
    21301 aaaatggatt ccgatggcaa cagaggaacc atctgtcatt gcagctgcaa gtaatggtgc
    21361 aaaaatctgt ggaaatatct gcgcagaaac acctcagcgg ctgatgcgag ggcaaatcgt
    21421 tctatcagga aaatcggagt atcaagcagt gatcaatgca gttaatcatc gaaaagaaga
    21481 gctgattctt tgtgcaaatg aaagttatcc ttcaatcgtg aaacgcgggg gcggtgttca
    21541 agatatctct actcgggaat tcatggggag tttccatgca tacttatcta ttgatttttt
    21601 agtggatgta aaagatgcaa tgggcgctaa catgatcaac tcgattcttg agagtgtggc
    21661 aaacaagtta cgggagtggt tccctgaaga agagattcta ttcagtattt taagtaattt
    21721 tgctacagaa tcattggctt ctgcctgttg tgaaatccca ttcgaacgct tagggagaaa
    21781 caaagaaatc ggtgaacaaa ttgccaaaaa aatccaacag gcaggggaat atgcgaagct
    21841 tgacccttac cgtgcagcaa cgcacaacaa agggatcatg aatggtatag aagctgtagt
    21901 ggcagctaca ggaaatgata cacgtgcggt tagcgcttcg atccacgcct acgcggcaag
    21961 aaatggatta taccaaggat taacagactg gcagatcaaa ggggataaac tagttggaaa
    22021 actaacggtg cctttggctg ttgccacagt aggcggagct tccaatatcc tgccaaaagc
    22081 aaaagcatct ttagctatgt tagatattga ttcagccaag gaactagcac aagtaattgc
    22141 tgcagtaggg ttagctcaaa acctggctgc tttgcgtgca ttagttacag aaggaatcca
    22201 aaaaggccac atgggacttc aagctcgttc attagcgatc tcaattgggg ctattggtga
    22261 ggaaatcgag caagttgcga aaaaattacg tgaagcagaa aagatgaacc agcagacagc
    22321 tatacaaata ttggaaaaaa tccgggaaaa ataaaaaagc aatatcttta gaagaataaa
    22381 gcaagattta cagttatttt atttactgca gatcttgctt ttttctctat cagaaaatat
    22441 gtttattatt agaatttttt atagttaagg ttaggcatct taagaaatca ggcaactcca
    22501 agatatcttc aggtagaaga gaagtcgcct aacattgtag gatcataggt gatttgttgg
    22561 acaggtcaag ctgatatttg agcctgatgt tagaaggaat ttctttttac ttgctgtaga
    22621 aattattata tgactaaaaa ggctgggatt ttaaatccca gccttttatg acaaatagaa
    22681 tttttatcga tcagtaatcg ctgtcgctgc aaattgttcg atcaaaggaa tcaaagtcaa
    22741 tttgactttg tatttccgtt cgagtgcttt ttcgataacg aactcatttt cagtagtcat
    22801 agaaatagtt gctaatttac gaggttcatc agcatttttt tcattaataa ctacttcatg
    22861 ttcattttgt tctgtgtaaa atttaggata ctcagcaacg gcttttgaaa aatcagcaaa
    22921 acttaaagcg gcattcgtat cagttgagcg tgtattttca gttgtgtccg cttttggttt
    22981 ttccgttttt tcttctggca cactgactgt ttcttctgta actggtgtcg caacagcttg
    23041 cgcagttggt tcatcgtttt tcaatcgttg atcattccag taagacacga tttcaataag
    23101 aagattgtca cggaatcgtt cgaaaatccc gttttctcct gaacgaattc tctttaatac
    23161 agtattgaag ttgtcatcga ttggcacgtt gatccgtgta tcttcatttg ttttcccagc
    23221 taatccgatc gttaagattt gacggttttt gggatcaagc atgacttgta acggcaaacc
    23281 ataacgtcgc aagctctctt cttctagaga acggaataat tgtcctctta caccatccaa
    23341 tgaaggacgg aattgattta ataatttttg tttctgtttt ttcttcatat gttctctcct
    23401 tcgctcatac cctttcgatt ataagaaaaa aacagccaaa tgaaaaggag taatgcaaaa
    23461 aaacttgcgt atacgcaaat tacaacagaa aaatttgcgt attttcccgt tttttgaaag
    23521 aaaaataaaa aatggaacgg attatgtata tatacataat ccgttccatt ctttctattt
    23581 tttcggtatc aaggcaaaaa caggtagttc aggtgaataa atgacttcat gttcgtgcca
    23641 gggtttgacc attagcgaat gatcgaaccg ttttttcagt tcttcgatga tttgatcatg
    23701 tgtccagttt cttgcaggaa aatgttggat tttctctgca tcgatcaaaa agacaaaatc
    23761 ggaatgaaaa gtctgggtaa cttcttccga gagagaattc aagcgttcga ttggatccat
    23821 aaaatcacct ttcgacttaa aattgggtag tatctgggtc ttttgctttg ccgactacac
    23881 catctaactg atcgatttgt ttcatttctt cttctgttaa ttcaaaatca aataattcag
    23941 tattttcttt gattctgctt ggtgtcactg acttaggaag aggaaggaat tcgtgttgca
    24001 gcgaccaacg caaagcaact tgtgcaatcg tcttgtcatg tgcttcagcg atttgtttca
    24061 tttcaggcac atcgaagatt ttaccagtgc ctaatggact atatgcttcg aggatcatat
    24121 catgtttttt tgcatactca actacagttg tctgtaattc gcctggtgca aggaaaactt
    24181 gattgaccat cggcataatt tttgctgttt ttgctagtgt atctagatgg tggggtaaga
    24241 aattgctcac accgattgct ttgattttac ctgcttcata cagttcttca aatgctcgcc
    24301 atgtatctgc atttgcttgt tcccaattat cacgaaaagc aacagggttt ggccagtgga
    24361 tcaaaaacag atctaaataa tctgtttgga gcttcttcaa tgattcttca aaagaagaca
    24421 tcacaagttc atagctgtgg tttgcattcc ataattttgt tgtcaagaat atctcttctc
    24481 taggaattcc actttcctta atcgcttggc cgacgctttc ttcattcttg tatccttggg
    24541 cagtatcgat atggcgataa cctgcagcca gcgcttcttt aacagaggaa acagcgacat
    24601 ccccatcagg tgtttgccat gtgccgaatc ctactaccgg gatttcgtaa ccatttgata
    24661 gacgataagt gcttgttaat gaattcatac tttcagctcc ttttttattc gttcaactat
    24721 aagtgtagcg catttttcga aaaaaagaat ccattttgtt tctactcttg aaatttttgt
    24781 ttaaaagaaa atgattttga ttctggtatt tttgattttt tttccgtaaa ctaagacaga
    24841 agggaatgag aaaatggata tctatttaaa aaaagcaatt ctgcatatta ttgacagaga
    24901 aactggttct ccagtctttt cacaaaaaga gctggatctg acaaaagaat atattcgtga
    24961 ttttcttcaa aagaagatcc aaaaaatctc ttctgctcaa acaaaaactg gacaactagc
    25021 agaagaaagc atgatcgttg aatcacttaa aatcgctcaa gaaaattttg tggaagcaag
    25081 tgaagcaatc gttcagcggt ggttcgatat ctaccaagaa agtgaagaag cacctagtgc
    25141 agatgtattt gttgtcttgt atgaattaga tacagtgatg tacttatctt tattgaaagt
    25201 caattatcgt gaggcttaca ctcactttgt tgaagcagac gaagcaggga tcgacaaccg
    25261 cttgattttg aatcgagcaa ttctaggggc gaaaacacaa aaagcagatg aagcattcgc
    25321 tgtgaatttg actgattgga cctacgaatt aattgaaaaa aaatatgagt tttctggcaa
    25381 aaaagagctt tatttttctg gaagagtgat cgaaagtgtt cctgcacctt ctttagaaga
    25441 aaatgtaaaa gtgatccaaa aagtagccaa gcatcttgga aaaaaatttg agacagaaga
    25501 atttgatatc atggctgatt tgaaagaagc tgtctatgat acgatagaag aaaaaggtcg
    25561 gttggatcat gagatgattg cggaaaaagt cttcaaggaa aacatcacgg caaaaatggc
    25621 ttttaaagaa gaagtacaag agcaaggatt tgtcccagaa gcacctcctg taaaagaagt
    25681 acaagaaatt tctgagaaaa aatttggaaa acaaaaattc aagctatcaa acgggatcga
    25741 actgatcgta ccagtagatg tatatcgcaa cccagatttg attgaatttg tgaataatcc
    25801 ggatggtacg atatccgtga tgatcaaaaa tgtggatgaa gtattgaatc gtttataaaa
    25861 atgactagaa tattaggtgt tcaagtacca gtgatcagtt aaatgaattg tgtcaagaga
    25921 aggatcggaa aagacgaata taacaaaaaa agagcgtgag acaaaagtaa agaacacttt
    25981 tgtctcacgc tctaaacacg tataaacggc ggaagcagaa gcaactcctt cttatttctt
    26041 ggagttaaac gcttctgtcc cagcctcttt ttatctaata aaattttaag accatttact
    26101 aattatcgat tttttctgcc gcatttttag gcacttcaga agcctttatt tcttcccagc
    26161 ttaatacacc tttacgagga ttgactttga gtttaagata cgcattcatc tttaatggat
    26221 gttctctttc ttcgcgaagc gtcacctgtt ttttttcacc atctttatta taagccgctt
    26281 gcttgtaggt ataagtcgtg aatttctctc cgttatctgc ttgggtagct gacatttcgc
    26341 cattcgtcgt gatttttgta taataagctt cgccgccgta aaaataatga taagctgcaa
    26401 aacttccgcc gagaaatatg cctaaaacga caagtacgcc aattaatttt ttcatgtact
    26461 tcacctcttt ccttattatt aacgaaatag aagatttcgt ctattgaaag ttgttggcaa
    26521 cagctgaatt ttaaactttt cctcattttg ttcttattgc ttcaaaagaa agttaaggac
    26581 tatcttgaag tttttctcaa aaaacacatt tttttattta taaaaggtac ttttttcgcg
    26641 tataagacag acgagtgctc ttagttgact ttgaccaata ttgacgtata ataagcttag
    26701 ttcgaatctt acaaaaagta agtcggacgt attctttttg atttagtcaa aaaaagacta
    26761 taataaaaag gtgagagctg aaatttttca gtgatctata ttatttagga ggtttgtaaa
    26821 ttatggcaaa agaacattat gacagaagta aaccacacgt taacattggt acgattggac
    26881 acgttgacca tgggaaaact actttaactg ctgcgatcac tacagtatta ggcaaaaaag
    26941 gcctagcaaa tcctcaagac tatgctagta ttgatgcagc tccagaagaa cgtgaacgtg
    27001 ggattacgat caataccgca cacgtagaat atgaaacaga aaaacgccac tacgcgcata
    27061 ttgatgcacc aggacacgcg gactatgtga aaaacatgat tacaggggca gcacaaatgg
    27121 atggtgcaat cttagttgtt tctgcaactg acggtccgat gcctcaaaca cgtgaacaca
    27181 ttttattgtc acgccaagtt ggtgtaaaat acctgattgt tttcttgaac aaagttgatt
    27241 tagtcgatga tgaagaattg atcgatttgg tagaaatgga agttcgcgag ttattgagcg
    27301 aatatggttt cccaggcgat gacactcctg tgatcaaagg ttccgcatta aaagcattgc
    27361 aaggcgatcc agatgctgaa gctgctatta tggaattgat ggatacagta gatgaatata
    27421 tcccaacacc agaacgtgat acagataaac cattactatt gccagtggaa gacgtcttct
    27481 caattacagg tcgaggaact gttgcctcag gtcgtattga tcgtggtgct gttcgtgtcg
    27541 gtgatgaggt agagatcgta gggatcaaac ctgaaacaca aaaagcagtt gtaacaggtg
    27601 tagaaatgtt ccgtaaaacg ttagattacg gggaagctgg ggataacgta ggcgtgttgt
    27661 tacgggggat ccaacgtgac gatatcgaac gtggacaagt acttgctaaa ccaggttcca
    27721 ttactccaca tacaaaattc aaagcagaag tgtacgtgtt gacaaaagaa gaaggtggac
    27781 gtcatactcc attcttcaac aactatcgtc cacagttcta cttccgcaca actgatgtta
    27841 caggaacaat cacattgcca gaagatacag aaatggtcat gcctggtgac aacgtgacga
    27901 ttgatgttga cttgatccat ccaatcgctg ttgaaaacgg aacgacattc tcaattcgtg
    27961 aaggcggacg gacagtaggt tcaggtatcg ttacagagat cgaagcataa gaaacgcgac
    28021 tcgaatattt tggtaatgaa aaatgtttaa aataaaagag cggtccaaac aggatttctg
    28081 tttggaccgc tctttgttct tcaattgtat aagaagagat tgtagaagca tgaactttcg
    28141 ttttacttca gtctctttta ttcatcatga gagtgttcat ctatcggtat gcagttttta
    28201 atactttctt cgtatccatt ttttcaaacc acttattcta ccttgtgtct gctcaactgg
    28261 cgcattatca ataatcagca aagcaatcat aacatatgca atccaactat ctctagaata
    28321 tagagtataa cgtgagaccc agtttgtagc gtacttgctt ttatattctc ttaatccatg
    28381 gaaagaataa aaatgagaac caaactcgta aaccaagtat gcaatccttt cctgtaaaaa
    28441 actctttctg gaagttccga catttgaaag aggggccatc cctaaattga accattggat
    28501 atgttcttct ttcatatagg caaacaaatg caagaacaag aaatccatac tgccagaagg
    28561 tgctttttta gaatcatatc gcatcagatc aatcgttcct actttattgt tcgtgtaagt
    28621 aggcatgatc gttgcaaaag cgacaatttc ttcttgggta tttttgacaa ctgcaatagg
    28681 cgctctttgt agataatctt cagagaaaaa gcctaaagaa aacccttttt cctttcgtga
    28741 acccaaccaa ttatcagaga tatttttgaa agtactcatt tgctcagtag aaaatggtgg
    28801 ttgtaagaca tcaaatgtaa atccttctcg ttcgatccga ttcaagacag cacgagtgcc
    28861 cttcattttt ttccctgaag ttgtaaaact attcaggtct acataagctt cttcccccat
    28921 cttaatgaaa tcgtagccga attcatgcaa aatcataaca atctcttcgc ttgtttcgta
    28981 gaaaactggt aaatagcata gtcgatctgt ttcttcgata aaagcttcga ttgcttctgg
    29041 aaaatcttct ttcttaccag aaggatcacc cattacaata catttattat tgaagcaagc
    29101 gaattggaga agaacagttg gttcaccgtc tttttcatag gcaaacatcc gcttatcttt
    29161 taagaagatc aattgactat cggagtttcc tccataagtt gtaaggatct tcagggcctt
    29221 ttcttcatta aaggcttcgc caatctgttt cttttctcct tgaaggaaat gaacaaatag
    29281 aacgatcatg aaactaacag cgatgatggc caacaatccc gaaaaccaaa tcttttcaga
    29341 aggaaataag aaaaaggaaa taaaatggtg acgccgatga gggaaatctg gcagattgta
    29401 taccccaata acgatgtaaa gcaaggacag tgcaccaata atgattccat cgatcgtacg
    29461 ccactcccat gaatagatca attgttcccg aaataattcg tttttagaag caagaatgat
    29521 cagaagcaga atagacaaga aaaccaccgg tgtgaaacta aaatcactta ataaaacata
    29581 aagtaaagct agaacaatca agaagattgt aggcaaatat gctcgtttca cacgtgctgc
    29641 gatccctctg cccataatga ttaaaagaaa tcccaatagt atactaggaa attggataat
    29701 aaaatgaaat ttcaatgggt tcaaactatg aagccagcga aactctgtaa aagcttgcgg
    29761 gatagtggca gaaagtacta gcatgattcc agaaaagtat aataagacaa ccacgatttt
    29821 atgcgcaatt tctgttgcta attgtttagg gataccagaa taacgctgat caaaagtgga
    29881 accgatattt ttgaagaaaa agacgattcc aatcaaaaac ggaatgatgt agtagaacag
    29941 gcgatagagc aagatccaca caacgactat ttctcttggc acaccaatag cggacaaccc
    30001 gataatcatc attacatcga aactcccaat ttctcctgga atcatagaca cgattcctat
    30061 cactgaagca gcaatgaata atggaattgt ttgccacaga tcaatccgga tttccattaa
    30121 ataaccaaca gaaaggaaac taaacaggac accggtccat tccaacaatg aaactagcaa
    30181 tagctgtcct tgagttttaa gtgaggtacc accaaggaat cctttctttt tgaaagtagt
    30241 aaataaaaag acgacaggaa aatatagacc gccgccgatc aaccatatcc aatattgctt
    30301 caaaaaagga gcaactggcg tgaagactac aagtaaaaaa gaaagcagac tgtaaataga
    30361 aagtccagcc atcaaaaaca agaaaacttt cgataatgct tggatcacgt ttctgctctc
    30421 tttcttttgt ccatatagtt cagagcgaag ccccacacta acaaacccgc cgaaaccggc
    30481 aatattgttg atcgtattga tcagccagct tgtctcaaac aggtaacgag gattctgctt
    30541 ttgttcaagc atccgattca aaataatatc ataaccaacc ataggtaaca cagagataag
    30601 accaatcaat agcattagtc cgcttttcca tacaggtata gtggcaaacg tttctcctaa
    30661 ttgctggata gatagagttt taccgatgga catcaattca ccgacaataa tgacaatgac
    30721 agaaatcaaa aagattgttt tgaacagtcc taggtggttt ttcagccatt gtatggtatt
    30781 ttttaacaaa taaatcacct tctattccta atagttatgt ttcagaaaga aattgaagaa
    30841 cttctcgatt gaagcgttca ggatcttttc tagctaattc atgtccttgt tctttcacaa
    30901 gcacaaagga agcttttggt atagttttag ctatatacaa agaatgtttt agcttgatta
    30961 catctttttt tcccacgatg atcaaagtgg gggagttgat tttttttaaa tcattttctg
    31021 taagcccgat gtcatgcagc aaaagtttga taactaaaag atttttgcga agacttgggc
    31081 gaaataaaga gagtaaccat acccatgcat aatgaatatt actgatgact ctggcagaaa
    31141 aacgaacgcc tttaacaagc gtgtttccag aatttaaaat caatcgatga acttttttag
    31201 ggaagctgct ggcaaatacc agtgctagat ttgctccatc actaaatcca agaaaatctg
    31261 cctgatcgat tttttccagc aacatgatcg tattcagatc ctcggccatt aactgaaaat
    31321 tcaacataga tgcttcgttt gtcgaacgac catgtcctcg gctatctact agatacacgg
    31381 tatagtagcg ttcgagtaca gggacctgtt ctgaaaaaaa gcggccgctg ccgtcattgc
    31441 catgcagtaa aaagagaggg aagccttgac cgctcttttc atagtaaatt tcgctcccat
    31501 ctggcatttt tgcatatttc ttttcttttt tcacataatc acctactgtg taaagtataa
    31561 cgattatatc agtcattggg caattgtttt tactgaaaaa caacgatcat tttcgtaaga
    31621 tgtcaaaaaa tacaggaaaa ttcatttttt cttaaggcgt acacagaaat caaggaagaa
    31681 atagccaaga agataaagaa atagtaagat aaaaaaagat gtcaaaataa ctaggtgatg
    31741 aaatgaagcg aaaacgatta tctattatcc gtattctttt ttacatgatc attctgacgg
    31801 ttattggtgc ggtgttgttt ttaggaggaa gaacttatcg ttcggtaaaa cgagtggaaa
    31861 gctatcaaat agaaataaca acagctgcaa aaaattatca tatggaaaac tacgagaatt
    31921 tgatcaaggc gataatattg accgaatcaa aaggaaaagg gacagatctc atgcagagct
    31981 cggaaagtct gcatggagaa gctgagatca ttgataatcc agctgacagt attgatcaag
    32041 gtgtcaaata tttggcggaa atgatagtag aagcaaagaa acaaggatgc gatttatgga
    32101 cagctgtcca agcatataat tttgggaaag attatattgc ctatgtaaaa aatcatggcg
    32161 gagaaaacac agtagaatta gcagaagcgt attctaaaga tgtgctgagc ccacttttag
    32221 gaaatgagga agggaaacaa taccgatatt ggcggatcca gtccttgctt tacaatggcg
    32281 gctatctcta tcataatgga ggtaacatgt tttatcctga tattgtcaaa atgaatgaaa
    32341 aaataataga gacctatgaa aaagtcttta agtaaaatag caggaaatgt tcaatcatga
    32401 ataaaattaa atgaaagaag gagtgcgcaa atagtggaaa aaaggcaaaa aatggaaaat
    32461 caagaagtgg ggtacacaac caaagactgg atgttacggt ttgtgaaggg aatgtttatc
    32521 ggttcaggat ttatcttgcc aggagtcagc ggtggtgcat tagcagctgt ctttggcatg
    32581 tatgagcggc tgatttcttt tttagcccac cttacaaaaa atttcaaaga aaatgttttg
    32641 ttttttctgc ctgttggtct aggcggagtg acaggcatct ttctactttc ttttttagtc
    32701 agttttttat taggtgcgta tgaaacaacg atattatggt tttttgtcgg atgtatcgct
    32761 ggaactgttc ctgcgttatg gaaagaagca gggaaaaaag gaagagatac ttctgatgtg
    32821 atcatcatgg ttgttacttt tgttctggct tatttgtttt tattatacgg cgctcaatta
    32881 tttgacgggc aagtcgatca gaattttgga acatggctga ttgccggcgg attgattggt
    32941 ttgggtatga tcgtcccagg attgagccct tctaattttt tggtgtatat gggattgtat
    33001 aaagcgatgg ctgacggatt taaagaagta cgacttgaag tgattgtgcc gatcggaatt
    33061 ggtgggattc tttgtgttct tggattgtca aaaatcatgg attacatttt ctcaaaggca
    33121 tatagcaagc tattccattt cattttaggt gtagtttttg cttcaacgat tatgatcatt
    33181 cctaccgatt acgcgggatt gggagttgct ggggtactag cttgtcctgt tctttttata
    33241 gccggtacag cactaggttg gtggatgagc cgtttggagg aacaatataa gtaaagatag
    33301 attgataata aaaacagcac acgggtgatt caaacaaaaa ggttggcatc ttttttgtaa
    33361 aatcattcgt gtgtttttct ataaatttta taaaaacaag ttgtttttaa tcttggaaca
    33421 cgctagactt aaacacgtga ttatttttga aagttggcgg aaaatggaga aaaagaaaat
    33481 gaatggactt acacagcaag aggtagttca gaaaatggct tctggccagc aaaatgacta
    33541 ccaggaagat gcatcaaaaa gtacaaaaca aatattcagc gataatatat tgacgttatt
    33601 caattttctg aactttggta ttgggatttg tctgttattt gtaggtgcgt attccaacat
    33661 ggcgtattta gcaattattg tcgtcaatat cgttattgga atttatcaag aaatacatgc
    33721 aagaaatctt gtacggaaat tatcgattgt ttcccaatca gaagtactag tgatccggga
    33781 cggtaaagaa caatcgattt ctactaaaga gctggtatta ggagatttgg taaaaatcgg
    33841 agcaggagaa cagataccag ccgatttaca ggttatatct ggacgggcag aagcaaatga
    33901 agctcttttg acaggcgaat ctgatttgat tgtcaaagaa actggagctg aattgctttc
    33961 tggaagcttt ttgacaagcg gttctgtcat ggctgaagtt atccgagtag gtgcagataa
    34021 ttatgctgtc cgcttgacta gcgaggcaaa gactcacaaa gctattcgtt ctgaacttgt
    34081 agattcgatt cgcaaagtat ccaaattcac tagtttcgtg attattcctt tggggatcat
    34141 actatttttg gaagcactgt tcatccgcga tgcttctatc aaaatttctg ttattgcatc
    34201 tgcagcagct ttgttaggca tgcttccaaa gggtttggta ttgctaataa gtattgcatt
    34261 aacgacaggg gtaaccaaac tggcaaaaaa acgaatcctt gttcaagaca tgtattcaat
    34321 tgaaacgctg gcccatgtcg atacattatg cttggataaa acagggacca ttactgaagg
    34381 gaaaatgagt atccagcggg tagaagcact acatccgctg tacgagaaag ccatcccaga
    34441 aattatgggg acgtatctgg cagaaagcag tgataacaac atcactatgc gggctatgag
    34501 agattatttt gagctttcta atcggtatga tgtgacaaac aaagtcgcat tttcttctga
    34561 tcgcaaatgg ggcgcgatgg aatttccaga actgggagtc gtgtacgtag gagcgcctga
    34621 acgtttggtg gaagaagatc ggcttccgga cagtctaaaa acagcccaag cgaatggcta
    34681 tcgcgtactt atgttgggga tcgctccaaa tcagcaattg aacgaagaaa aaccagcatt
    34741 tgttgagccg gttgctctgt ttgaaatcga tgatccaatc cgaaaaaacg ctcaagaaac
    34801 actagcttat cttcatgagg aaggtgtgga tctgaaagtg atttctggag acaatccttt
    34861 tactgtctcc aatatcgcta gacgtgcagg attaccgaat tatgatgcgt atgtcgatct
    34921 ttctcaaatt actgaagaaa tggaagtgcg agaagctgct catcgctata cggtttttgg
    34981 acgagtgagt ccgcagcaaa agaaattgct ggtaaatgaa ttgaaagaaa gcggcagaac
    35041 ggttgcaatg acaggcgacg gtgtgaatga tgttttggcg ttaagagaag cagattgcag
    35101 cattgctatg gcagaaggtg atggagcaac acgtcagatt gcgaatctgg tattgttgga
    35161 ctctgatttt actacattgc cagaagtcct gtttgaagga cgccgagtgg tcaacaatgt
    35221 gacgaaagtc tcaggcatct tttttatcaa aacgatttat tcatttattt tatcgattat
    35281 atgtgccgtt acagcaattg gatttccgtt tatccctatc caaatcacgc tgattgattt
    35341 agcgattgaa gggtatcctt ccttcttttt atcttttgaa caagataaaa gaaaaatcac
    35401 ttaccgttat ttgcctacag cacttattaa tgctttacca aatgccttgc ttgtagtagt
    35461 gaatatcatt gcggtgtatc tacttggaaa aacattcgca tggggaaatg ctgaaactac
    35521 tactttgatg tattacttgc tgattgggat cagttgcatg gcagttgtaa aagcttgtta
    35581 tcccttcaac ccattgcgca tcttcctagc agtaacgacg atcgtaggga tatatgtagc
    35641 tgcaatgctg ttccatggaa ttttggaagt aaacttaggg ttgggagaga gcgtaggata
    35701 cttctttgta ttcatgctcg taaatattgt tcttcgagta ttgtactgga aggctgatat
    35761 tcaaaatcag ttactgaaaa gagtagcaaa ttgatgctta atgagataga aatgtgttat
    35821 acttgaaaaa gcaatgaaat atgatacaat caaacatgta taattgtgtc ctttaaaatc
    35881 gcactgattt cagttagtgc gattttttac cggttattct cctgaaatat cttctgttaa
    35941 acccaaaaga tatcaataga gcagggggtg aaaaaatggc tcaacggatt cctcaagagg
    36001 ttatcgaaac agtacgcagt caaacgaata tcgttgaggt gattggacaa tatgtccagt
    36061 tgaaaaagtc tggtaaaaat tacttggggc tgtgtccttt tcatgaagaa cgtacaccgt
    36121 ctttttctgt tgcagaagac aagcagattt ttcattgctt tggttgtggt aaaggcggaa
    36181 acgtgtttac ttttcttcaa gaactagaag gtctaagttt tccagaagca gtggtaaaag
    36241 tggcagactt cgaacagata ccgttagaag atcaatggcg tcagcgtact atagctgtcc
    36301 aaaatcctga atctgctacg gggaaactga ttgctgctca tgaaaaagca gcggaaatct
    36361 atcatcacat gctattgcat acaaaagctg gacagccagc tttagaatat ttgcaaagtc
    36421 gtgggttgtc tttagaatta attgaagaat tcaacatagg ttttgcacct aatgagcgtt
    36481 cttttcttca gcaagtattc aagaatgaat caatttcaaa tgaattgtta gaacgctctg
    36541 gattgtttgt cacgcatgat gatggccgta tgtctgatcg tttttatcaa agagtgatgt
    36601 ttccacttcg taattctcaa ggccagacga tcggtttttc aggacgctgg cttgcacctg
    36661 aaaaagggca ggaaaaagat cagcccaaat acttgaatag tccagaaaca gaactgttca
    36721 ataaaagaca agttttattt aatcttgata aagcaagaag cgatatccgg aaaaacggag
    36781 aaattttgtt gttcgaaggg tttatggatg tgattgctgc atggcaggct ggtatcaaaa
    36841 ctggattagc ttctatgggg acaagtctaa cagctcaaca gatccaacaa atggaaaagc
    36901 tgacgaaaga attggtcttt tgctatgacg gagacaacgc aggttttgaa gcaaccaatc
    36961 gagggattga attgcttcgg caaaacagtc gattagcact ttctgtcgta gtcgttccag
    37021 aaaagcttga tcctgatgaa tatctccgta agtatggaga agcatctttc caagaattag
    37081 tggctcatgg gcgcgaaaca gtctttactt ttaaatcacg ttatttaaaa caagggaaaa
    37141 atttagataa tgaaaaagac aagatcgaat acctcgaaga attactagtt gaacttagcc
    37201 gagttgtttc accaatcgaa caagatatgt atctatctca actgtcaaca gagtttcaga
    37261 tcggcagaga gacgatacaa aaacaactgc gcgatcttcg aaggacaaat cagcaaaatc
    37321 gtcaagaacc tcaggaaagt caacacactc aggttacgag acaagaaact gcacggaaaa
    37381 agagaccttt gacgcaagta gaaaaggcag agcagattct attatatcgt gcatttaaag
    37441 aagtttctgt ccgtaatatt ttaaaagaac gagaatttca gtttattcat gatgcatatg
    37501 cagaagttta ttttttattc gatgcatatg tctcacaaca ttatgaattc aaccttgcag
    37561 agtttttaga ctttttgcaa gatgaaaatt taaggcggtt ggtggtggaa atcgaatatt
    37621 taccggtggc agaagaaagt tcgccacgag aaatccaaga cttgctagat gtgatcagca
    37681 aatctggctt agctgatgaa ataacattaa aaaaacaaat gcagcaagaa gccagacgaa
    37741 ctggaaacaa acagcttgag cttgaattga cgattgaaat catcaattta acgaaacagt
    37801 taaaacaagc caaatagcta ctcaaggggg gccttctttt atggcaaatg gaacagataa
    37861 aaaaacgtat gaagcagcag tagcagcttt catcaaagaa aacaaaccca aacgacaagt
    37921 tgtatatgat gatttatcca atcagcttgc tacaccattc actttgaatg cagatgaaat
    37981 ggacaaattg atccaaaaga ttgaagacgc aggtatcagt gtggtggatg aaaatggtga
    38041 tccatcaatt cacagcttga agagtgctga gaaaaaagct gaacaagcaa aaacagaaga
    38101 tctttctgcg ccaaccggtg taaaaatcaa tgacccggtt cgtatgtatc tgaaagaaat
    38161 tggccgtgtc tcccttttaa ctgcggaaga agaagtagcg ttagcattga aaatcgaaga
    38221 aggcgaccaa gaagcgaaac agcgtctggc agaagcaaac cttcgtttag tagtaagtat
    38281 cgctaaacgt tatgtaggac gaggcatgca gttccttgac ttgatccaag aaggaaacat
    38341 gggactgatg aaggcagttg aaaaatttga ctaccgcaaa ggatttaagt tttcaaccta
    38401 tgctacatgg tggattcgtc aagccatcac tcgagcaatc gctgaccaag cacgtacgat
    38461 ccgtattcca gttcatatgg ttgaaacaat taataaattg atacgtatcc aacgtcaatt
    38521 gctgcaagac cttggaagag aacctacgcc agaagaaatc ggtgcggaaa tggatcttcc
    38581 aacagaaaaa gtccgagaaa tcttgaaaat cgcgcaagaa cctgtttccc ttgaaacgcc
    38641 aatcggtgaa gaagatgact ctcacttagg tgatttcata gaagaccagg aagcaacaag
    38701 tccagcagaa cacgcagctt atgagttact aaaagaacaa ttggaagatg tactagatac
    38761 attgactgat cgggaagaga atgtccttcg cttacgtttt ggtttagatg atggccggac
    38821 gagaacattg gaagaagtag ggaaagtgtt tggtgttact cgggaacgta ttcgtcagat
    38881 cgaggctaaa gcattaagaa aattgcgcca tccttctcgt tcaaaacaat taaaagattt
    38941 cttagaataa gcgtggacta ttgaccagaa gagaaatcag taaaactgtt tggtttattt
    39001 taaaagaaaa aaagcttgag actacgcagt ctcaagcttt tttttctata ggaaggaaat
    39061 tcattcatta tttttaagta aatcctaaaa ataagctatt ggcgtgacgt atagaatatg
    39121 atagattaaa tggtagcgta aacctagtaa aggagccgat cgctaagtga tgaaaaaatg
    39181 tccaaattgc ggatcgaaaa aaatggagca gaatactatc tgtccagact gcggtcatca
    39241 aataactgca gaagatcagc aaataaaaga atcaattgaa gaaacaaata cagatgcagc
    39301 tatgcaggat aaatcaactg aagatgcagt ctttcctgat acagaattaa acgatccaat
    39361 cgaatggtcg gaattgaaag atttacctct agaatctgta atggaattat tcgaaaaagc
    39421 agaaccagaa gacgaagaac cacctgctca cgatcaagta actaatatag agaaaaatga
    39481 acagaagaaa gcaactgttc atgagacttc ggaagaatca ggaaagaatc aagaagaaac
    39541 gcttgaatca aaacaaaaaa gaagagtagc tgaactgaaa gaaatcgtcg ataatgagga
    39601 agaaaattcg atactatccg cttatatcaa agcacatcgt gaagatacaa aagaagagca
    39661 cgcgaaagag ctgctcaaaa tgatcagtga aaaaattgca caggaaaaca aagtagagcc
    39721 agaaaaggaa caagaaactg aaaaagactc tgatagagaa aacgccacag gtaaagaaac
    39781 agtaaaaata aaagacgtaa aaacggatat cacagaaaaa actgaagtat ttactgaaaa
    39841 acaattagaa aaggaaaaaa cgaataaact gccggaagca gagaaaaaag aaatactcga
    39901 aaacacagac aaaaagtcaa cagagcccga tgaaataaat agtgaatccg atgctgaaaa
    39961 tcaatctgta agcaaagagg gtactgatct tgaagcaaaa atagaaaaag aagagaacca
    40021 gcctaagctg tttgaagcag aagcgaaatc aataacaatc gtgccagata ctgaaaaaac
    40081 acatcaagaa tcaaacttag aaaaaacaga tccagataaa gcgcaaaaga aaaaatcaaa
    40141 aaaagtacct tatctcgttt tagcagcagt acttctactc ggtggaggaa gctgggctta
    40201 ttatgaccat catcaaaaag tagaagccga agtagcagca gaagcaaaaa gacagcaaca
    40261 aatggaagaa cttcatgaga aacttgctgc cttttataca gatgatcacc atcaatttat
    40321 tcgtgcagat atgattcatc aagatttatc aaaactaaaa aatgatttat ccagtcttaa
    40381 gaatgaaaaa ggatattcag agttagaaac aacttaccaa gatatccaat ctaagatccg
    40441 agatatccaa aaagtgaatg agtggttcat agcacctgcc atcgtcgatg atcatctagc
    40501 agatgacatc aagttgaaag cagataaaga gatccaagca atcgaaacgg ataacacaga
    40561 ttttggaaaa ttgattcaac aagcacaaac agaagctaaa aatcaatatg ctgaattgca
    40621 aacagctaaa gaaaaaacag ctatagtctt taaagacgga aaagtagtgg attcagcaac
    40681 acgcgaccaa tacaagacgg caaaagaagc agtagaaaaa gtgaaaaata cagatctatc
    40741 tgctcagttg aatgaacaat tgaaacaagt agataagatg ctcactgata aagagaaaaa
    40801 agaagaagag gaaaagaaaa aagcagaaga agcgaaaaaa gcagctgaag aacaagcaaa
    40861 acaagccgct caagcagcag ctgcagctca agccgctcaa caagctgcgg tcgcacaagc
    40921 agcacagcaa gaagcggcat cctcgacccc ttcctcttca aactcagcga atcagccaat
    40981 caaatctgcc agagcaagtg atattgccga tgcatctaat ccagcatgga cgtgggcacc
    41041 aggtgtccaa agcaaagtga ttgctacatg tatccaacgt ggatatatcg ttgaaggagg
    41101 ttacagatta gaaaaagcca gaatcgaaaa tggggaaggc tattataatc tatatgccac
    41161 ttcaacaaaa tctgcattga tgaaaggcat tggagaaagt gctttgccat tttacatcgt
    41221 aaccatcaac tgtaaaactg gatggttcgg aggaaatggg agcaattaat tttaggcaaa
    41281 tgaaagaggc tgtgagcctg ccgtttaaca ccgtttattc gaaaaagggg agtatgacac
    41341 aactgttgtc atactccttt tttcaccttt ccaaaccaag gctcagctat tcaagtagat
    41401 aacattatgt tatgatagca aagaaatgta ttaagaaaac ctttcactat agagggagaa
    41461 aaaaatgaac gatttattag caactatatt tacaggtatt attattgatg aaaatgaaaa
    41521 aaactacttt gtccaaaaaa acggacaaac ctttggctta agcaaatcag aaggtgaaca
    41581 caaaatcggt gaagcagttg aaggattcgg ttaccaaaat caaaaacatg aaaatcggat
    41641 cactacacaa attcctaaaa gccgtatcgg tcattacgct tttggaatcg ttactgctac
    41701 acgaaaagat ttaggtgcgt ttgtcgatat cggtcttcca gataaagact ttgtcgtatc
    41761 tcttgatgaa ttgccgacaa tgacagagct atggcctaaa aaaggggatc gcttgatgat
    41821 ttcccttaga gttgatgcaa aagaccgtat ttggggcagt ttagcagatg aaaaaatatt
    41881 caaatcttta agcaaatatg gaacagaaga aatgaaaaac aaagatgtga taggaacagc
    41941 ataccgcttg aaattaacag gtacgtacgt tttaacggaa gatttctatc ttgggtttat
    42001 ccatccttct gaacgatata atgaacctcg tctaggcgag cagataaaag gtcgagtgat
    42061 tggtgtccga ccagacggtg tactgaatct ttcattgaag ccaagagcat atgaagctat
    42121 atcagatgat gcacaaatga ttttaaccat tttagaacgt tctgcagatc agcaaatcaa
    42181 ctttacagat aaatcggatc cagaagaaat catgaaagct tttggtatca gcaaaggagc
    42241 atttaagcgc gcattaggta atcttttgaa acaaggattg attacacaag aaaacggtgt
    42301 gacaaaacta gtcaaaaaat ctaattgaga atcccttgca tgtttttctt aactagctta
    42361 taataatttt aattaacata gtgttatatc tttttttatt tgtaattatt ataaattgtt
    42421 ggggttgagt aaatggtcat gaatgctatt tcggcgatga aaaaaacaaa acagcagcta
    42481 catgaagcgg ggtttaagct gacaccgcaa agagaagcta ctctgcttgt gcttttggaa
    42541 aatgagaaag agcatttatc tgcagaagaa atcttctttt tagtgaaaaa aaagagtccg
    42601 gaaattggac ttgctactgt ttatcgaacc cttgaaatct tgacagaatt gaaaattgtc
    42661 gataaagtaa gttttaatga tggattagcc cgttatgatt tgagaaaaga aggcgccaaa
    42721 catttccatc atcatttact atgcttgaac tgcggcacga tcgaggaagt agaagaggat
    42781 cttttacttg aagtggagca agtagtagaa gaacgttatc attttttagt aaaagatcat
    42841 cggctaacgt tccatggaat ctgtcaggaa tgctatgaaa aagaaaaaag aaaccagtcc
    42901 gtctgagtgt ctagacggct ttttctttgc tcagattatg gaaaaagact atcagaatga
    42961 agcggtatct gatatgatag agaggcatga gggggattat tatgaaagag caaatcaacg
    43021 attatctcca ctacttgaat attgaacggg gattgtccgc caatacacga caaagctatg
    43081 aacgtgattt ggaacagtac ttgcattttc tacaggagca gaaaattgct tcatggcagg
    43141 atgtcgatcg ctttttagtc atccgttttc tggagaagtt gcatgaagaa aaaaaagcga
    43201 cagcaactgt tactcggatg atctcaagtc tcagaagatt ccatcagttt ttacggcaag
    43261 agcgtatcac tgatcatgat ccgatgcaac atatcgatac gccgaagaaa gcacagaaat
    43321 taccaagtac attgactctg acagaagtag aaaagctgat tgaagcacct gatacgacaa
    43381 aaacgttagg gataagagat cgtgcaatct tggaagtcat gtatgctact gggatgcgcg
    43441 tcagtgaatt gattggattg aaattgggag atcttcatct ttctcttgga ctggtacaga
    43501 cagttggaaa aggagataaa gagcggatca ttcctttagg tgattatgcg atccaatggc
    43561 tggaacgcta cttagacgaa gcacgtccat tacttgtaaa ggacgcatct gaaatgcatg
    43621 tatttgtcaa taatcatggg aaaggtcttt ctcggcaagg aatctggaaa aatctgaagc
    43681 agttagtacg agaggctggg atttacaaag aagtaacacc acatacgctg cggcatagct
    43741 ttgccacaca tctgctagaa aatggagctg atttgcggac agtacaggaa ttgttaggtc
    43801 acgcggatat ctctacgact cagatttata cacatatcac gaagaaaaga atgacggatg
    43861 tttataagca gcattttcca agggcttagt cataggaagg tgagaagatg ttaacacatt
    43921 accggaaaga aaaccgtaaa attgctatgg gactattgag tttccataag aaactgacag
    43981 gtaaaacttc tttgttgaag gagattgata cgtacgaatc tgaagacgat tatgaacttt
    44041 ttttctttac gccagaaggt tcaaccaaca ttcaagggat tattggtatt tactgggcaa
    44101 cgaacgatca gctgatcatc catgacattt ctttgaatcc gtcttatcgc ggtgagaata
    44161 tgggctttca aatgttagat gaattacagg aaaagtaccc ggatgtctcc atccgtgcaa
    44221 ctgaaatcac agagccttac attggaaaat ggctagcaaa gaaaaagaag aaagagtgaa
    44281 tgagttgacg gagatcaata ttaaattaga tgtattcgag ggtcctctcg atctgctgct
    44341 tcatctgatt cagcaaatgg aaatcgatat atatgatata ccgatcgcta cgatcacgga
    44401 gcaatatatg aattatatcc atacaatgaa aacactcgag ctagctgttg caggagaata
    44461 cttagtgatg gctgctacat tgatggcaat caaaagcaag acattgcttc ctgtccaaga
    44521 attcgtcaca gacgatgaag aattatggga agaagatcct cgagaagaat tggtcactca
    44581 gcttttagaa taccgtaaat ataaatatgc agctgaaaga ctgacagaaa aagcagagga
    44641 acgaagtctt tattacacaa aagaacctat gaacatcgat gggttagcgg atgctgaaaa
    44701 gccgttgaag cgaggacaag tcaaaaaaat cgatttgatc cgtgccatgc agaaaatctt
    44761 agaacgtaag aaattttctc aaaaagtgga aaaaacgatc acgcccgatg acacaacgat
    44821 cgaagaacga atggtattta tcgaagagcg tataaaaaga aatgcgcatg gctgtacttt
    44881 cgattctttt tttgagtcac cttctaaaag tgaagttgtc actactttta tggctttgct
    44941 agaattgatg aaaaaagggc agatcattgc gcaacaaaaa gaaaactatg aatcaattat
    45001 gctttatcca gcagtaggag ataccgaaca tgacgaaatt aagtgaactt gaagcccttc
    45061 tttttattgt tggcgaggaa gggatcagta atgaagaatt aagcggactg ttaaatctca
    45121 gcgaagcaga agtcacaagt ttagtgaaag aattaaaaga atcttacact caaaactcgc
    45181 actcagcttt gcatattttt gaaacggaaa gccattatgt attaacgact aaaaaagagc
    45241 tggctccctt acttaagcga tatgcgcaag ggacatcaaa cacattatct caagccgccg
    45301 tcgaaacctt agcaatcatc gcttataaac agccaatcac aagagctgaa atcgagcaga
    45361 ttcgaggagt tcaggtttca ggagcggtac agcgtctagc tgcccataaa ctgattgaag
    45421 aaaaaggtag agtagaagga ccaggtcggc cgatattata tggaacaact ggctacttct
    45481 tagattattt tggtttgaaa tcattagaag aactgcctga tacacaagaa atggaaattt
    45541 cacttgaaga agacgtgtca ccagatctat tctttgatgg aatgaataaa ggagaataat
    45601 tgtaatggag agattgcaga aagtaattgc ccacgctggc atcacttctc gtcgaaaagc
    45661 agaagaattc atccttgcag gacgtgtaaa agtaaacgga caagttgtaa gggaattagg
    45721 gataaaagtt gggaaacatg acactgtgga agtggatgaa gtcccaattt atcaagaaga
    45781 atacggttac tatttattgt acaaacctaa aggagttatt tctgctgttg cagatgacaa
    45841 aggaagaaaa gttgttacgg atcttttgcc gatggtaaaa gaacgtatct atcctgtagg
    45901 tcgactggat tacgatactt cggggatctt gctactgaca aatgatggag attttgctca
    45961 gcgcctgaca catccaaaac atgaagtgga taaagtttac gttgcaaagg taaaagggat
    46021 cgctacaaaa tcgatgcttg cacctttgac aaaaggaata aagatcgaag gacgaaaaac
    46081 atctccagct aaattccaaa ttttatcagt ggatcctaag acaaaccaca gtatcgtgga
    46141 attgacgatc cacgaaggac gtaatcatca agtgaaaaat atgcttcaag cagttggtct
    46201 tcctgtgcaa aaactaaaac gtgaaaaata cggagatctg actttacaag gcctacgtcc
    46261 tggggattac cgtgaactga acaaaaaaga agtcagcagt ttgctgaacc actcaaaatg
    46321 aacacttact ttttataagt gttagatgtt gcgttttttg aatagatatg tataatgaat
    46381 atgtaaacaa aaattatata aggttcgtct tcaggggcag ggtgaaattc ccgaccggtg
    46441 gttatagtcc acgacctatt ttgcaaaaaa atagctgaat cggtgaaatt ccgataccga
    46501 cagtatagtc tggataaaga agatagagct tatttgacga cgcttgatca aataactcta
    46561 actctatttt ccctggaaaa tagagttttt tcatttggag agaaacgtca aaaagttcgc
    46621 tgaagaggag tcctaacagg aggatttcaa atgaagaaca cccgtgtaca gaaaatggtt
    46681 gcagtagcga tgtttgctgc gatcgcatta gttttacagt attttgcttt tccgatcatg
    46741 ccggctttta gttttttgaa gatcgatttt agtgatatct cggtattgat cagcatgttc
    46801 ttattcggtc cagcggccgg agttgcaaca gcgttcttgc gttcagtatt acatctgctg
    46861 actacaggtt tttctccgga taatttggta ggagacactg ctagtttctt atcaacaacg
    46921 atctttacat tcccgattta ttatttcttt agtgtaaaga aacagcataa aggaagaaac
    46981 aaatttttag gtgtcattac aggaaccatc gctctaacta tttttatgag tatcgcaaat
    47041 tatttcgtta ttacgcctct ttacttaatg tttttagggc tgaacgcaac acagatgctg
    47101 ggcatgtctt tagcaaacta tgtactgatt gggatcgttc ctttcaactt gatcaaagga
    47161 ttcatcgtga gtgctgtctt tttagtgctg catgcgcaac ttcttccatg gttaagtcga
    47221 aaacaacatc agttcgaaca acgtcataca gcagtcaaat aaatatcaaa aagatcaaga
    47281 aatttcttgg tctttttttt tggaaaaaaa tacaagacat gcaataatgt aaacgtttta
    47341 tttgtttgtt ttgagtattt ttttgattta tgcataaaaa tcaatcaatt ctttgcaaat
    47401 gtgaaattct tctcctttta aaagacgaaa atgtttgttt ataaaaaaaa ggaaacgcta
    47461 caatgaaaaa gaagaaagct ttaaaaacct gaaaaaagag gagggaatag ggaaaaacga
    47521 actttttgaa caaaagtttt acaaaatgcg tgttattatt tttgttctga tactcttttt
    47581 gataaaataa cattagagcc aaaattgttg tctctaacgg aaaagaaagg aagaagaaga
    47641 tggaacaaac aaaaaagaaa aaatttcaaa tgccttcagc gtatacggtc ttatttatta
    47701 ttattgcagt aatcgctgta cttacttggt ttataccagc tgggcactat agtgtagacg
    47761 atgcaggcaa catcgtcgct ggatcatatg aacgagtaca acaaaatcct caagggctgt
    47821 gggatatttt catggcgcca atcaatggga tgctaggtgt accagcgggg gagctaacta
    47881 aagaaacacc tgctgcaatc ccaatctctt tcttcatttt ggttgttggt ggatttttag
    47941 gtattatcaa caaaacaggg gcactagatg ccgggattgc ttcagttgtg aagaaattca
    48001 aaggaaaaga aaaaatgctt attccagtat tgatgctgtt atttgcatta ggcggctcta
    48061 cttacggaat ggcagaagaa acgattccat tctatgcttt actgatccca gtcatgatgg
    48121 ctgtaggctt tgatacagta gtagctgtag caatcgtatt agtagggtct caagtaggct
    48181 gtctagcttc aacggtaaat ccttttgcaa caggcgtagc ttcacaagct gttgggattt
    48241 cgatgggtga aggtattggt cttcgattct tgatgttcgt tattttgtta gctatttcta
    48301 ttgtatatgt gtatcgttat gcatcaaaga tcgaaaaaga tccaacaaaa tcattagtat
    48361 acaaccaacg taaagaagat atgaagcatt tcgatattga agcaatcggt cgtcaagaag
    48421 taatcacgaa aaaacaaaaa catgttaatg tattgttctt tattactttt ggtatcatga
    48481 tcatcagttt gattccttgg acaaccttga atgcgaattt cacgattttt gaatcattga
    48541 caaactggtt aacaaacctt ccggtactag gtacggttct aggtaaaagc atgcttccat
    48601 taggtgactg gtacttccca gaaattacta tgttattctt agtaatgtcc atcgtagtag
    48661 caatcactta tggcatgaaa gagtctgaat ttgtctctga atttttagct ggtgcttctg
    48721 atttgatcgg tgtagcaatc gtagtagctg ttgctcgtgg tatccaagtg gttatgaaca
    48781 atggattaat cacagacacc atcttacatt ggggcgaaca aggattaagt tcattatctt
    48841 caagtgtatt catcattatt acatttattt tctatatccc tatgtcattc ttgattcctt
    48901 caacttccgg cttagctgct gcaacaatgg gaatcatggg acctatgggt gaatttgccg
    48961 gcgtcggtaa ggatttagta atcactgctt atcaatctgc atctggttta gtaaacttaa
    49021 ttacaccaac atctgctatc gtaatgggtg ctgtagcaat tgctcgtttc gatgtttcag
    49081 tgtggtggaa atttaccggt aaattaattg cgatattatt tgtagccatc tgtgtgatcc
    49141 taggtgtagc agcaatgttc taaacctaga caaatacagg tacaaggtct atcataagaa
    49201 tgaagaaacc gataaacggc tatcgataag cacgtggata agtcaatagc tccattcagt
    49261 gagtgttttt tctttcatca tttgttaaga acattctgct tgctgatgga gcaggcttaa
    49321 aacgtcatta tttgagaaga agaggttgtg tcggaggaat ttgacatgac cctttttaac
    49381 atattaggag gataatatca tgaaaaaatt tgtgacagaa caacatcatg aacaagcatt
    49441 agaatcattg agcgaattga tccgaattcc ttccgtacta gatgaagcag attcaggaca
    49501 aggacatccc ttcggtaaaa aagtgattga agctttggat aaagtattgg aaatcagtga
    49561 aaagctagga tttagaacat ttaaagatcc agaaggctat tacggttatt cagagattgg
    49621 ctctggagat gaattattcg gtatcttgtg tcatatggac gttgttccag caggggacga
    49681 aaataattgg gaaacaaaac cattcgatcc aactataaaa gacggctggt tagttggtcg
    49741 cggatcccaa gatgacaaag gtccttctat tgcggcaatg tatgccgtca aagctttgat
    49801 tgatgctggc gtggaattca agacacgtat tcgttttatc tttggtacag atgaagaaaa
    49861 tttatggcgt tgtttagaaa aatataacga aaaagaagaa ggaattacgc aaggatttgc
    49921 gccagatgct gaatttcctt taatttatgc agaaaaaggc ttattgcaag cttacttgac
    49981 aggaccagga acgaacgagt tttctgtaaa agctggagga gcgttgaatg ttgttcctga
    50041 tgcagctcct tactctggag aaaagctttc tgaagtaaaa gaagcattga aaaaacacga
    50101 attcgatttc gaagaacaag gtgaaggcat cgtcgttctt ggtaaaagta tccacgcgaa
    50161 agatgcagca caaggagtga atgctatttc tcgtctggca attgctcttt cagaagtatt
    50221 tgattttggc ccaatcaatt tcttaggaaa attagttcaa gaaaatgcaa ctggtgaagc
    50281 tgtcgtgggc aaaacagaag atgaacaatc cggcgagcta acgatgaact ttgcaagttt
    50341 ggaaatcaca ccagaacaaa caaaaatcgg tgtagatatg agaatccctg ttacattcaa
    50401 gaaagatgat ttagtagcta aattaactaa aacagcagaa aaatatgggt tgacttatga
    50461 agaatttgac ttccttgatt cattatacgt gccactagac agtgagttag tgaaaaatct
    50521 attggggaca tatcgcgata ttactggaga catgactgaa ccgtttgtat caggcggcgc
    50581 tacttttgct cgtacgatga accagtgtgt ggcattcggt gcgatgttcc ctgatactcc
    50641 tgactttatg caccaggcga atgaacgatg ggaattaagt agtatgtata aagcaatgga
    50701 aatttatgca gaagctgtgt atcgtttatg tgctaaataa aaataaacat acaaaaaggg
    50761 cggatattca aatggatatt ccgtcttttt ttatttgatg ctggaaagtt tgcatttctc
    50821 tcttattttt ttaaggtata atcatacttg attaagaaaa aggggtcact cagatagaaa
    50881 aggtgggaga aagtgaaaat atttagacga atactaattg gcttagtcat cattttagga
    50941 ttactcggtg gagccggtta ttactatata cagcaaaata cgtacgaacc atcgtcagaa
    51001 gcgcaagtgt tggtaaaaga aggaaaaaac aaagaagatt acttattttt tgaaggagag
    51061 aaaaagaatc atggaaagtc tccattactt attttttatc ctggtgctct cgtagagcca
    51121 gaaagttata gtatttgggc gcaagattta gcaaaaaacg gcttttcagt cgctattgtc
    51181 aaaatgccac ttgatctagc agtgatgggc ggaaacaaag cggataaggt caaagaagat
    51241 tttccaaatt ccgattatgt gatcggtggc cattcattag gtggcgtaat ggcaagtcgt
    51301 tatgtcaata agaatcattc agacaaacat ttaaaagggg tattcttcat ggcaagttat
    51361 cccgacaaaa aaggaacatt gacagaatca gctgttcctg ttttatcgat cacggcgaca
    51421 aaagataaag tattgaatca ggaagcttat caagaagcta agcagtattt accggaaaga
    51481 acagagtatg tatcaatcaa tggcggaaat catggaggtt ttggcagcta tggtgagcaa
    51541 aaaggagatg gagaggcaga aatttctaac gcagaccagc aaactgtgat cgtcaataca
    51601 ttggaaaact ggctgaaaaa gttgaaataa tataaatggg tcctataggg tccgtgaaaa
    51661 aactagtcaa ttaactatag atagcaaatg aatctttcaa aataagtcgg gatttcacgg
    51721 aacaagagaa acagatcccg gcttattttc aagtttagag aacttagtgt acgtttttaa
    51781 agtttcgttt atatttatat tttattttct aataaaatcg ctcgaactgg acatttttga
    51841 tacgcttttt ttacatatgt ttcttcttcc ggcatcacga actgttgatg agcctctggt
    51901 tcttgggcaa ataaaacgat tccttcttca tcataatcga aaatatctgg cgcttcgatt
    51961 tgacaaagac cgcatgcaat acagcgttca ggtacaagtt tacatagtag tgacatttct
    52021 aaccatccta acttaaggag aataatatga ttgtttttat tttatcgcta ttttcatcag
    52081 gacacaagct gcggattagt tctctttacc aactccttgt gggaaaacga acgacttcag
    52141 tacttattta tggatttacc cacgaacttt tgtttatcca taacagtttt ccagaattga
    52201 agcaggataa gttttatcaa attttgcaaa aaattgcgca gcaaggttgg atcgaaataa
    52261 atgaaaatga agcaaagcta acgcctgcag gggctgatat gttgtccgaa catcaaatag
    52321 aacatactgg tttgcgtttt gaccgctatg gcagaacagg ggaaactagt tggcggctca
    52381 ttaaatttgc tgttcaagta atttctaatc tggcaacggg tattcaagac tatcttccag
    52441 cagagacaag tcctttttat actttccaac taaaaaaatg gcttagtgaa agccaattgc
    52501 cacgtgaaat tttgattgat actgcgtatg agagtttggt gcagctgttt tcggagatac
    52561 cagaagaagc agcagatttt ttagctaacc aattatctgg aaatgagcga acagggctgc
    52621 ttccttatca gctggcaaaa aacaatgatg agtcagcggt ttatctgcaa caaagcagat
    52681 gtatccacct tttgttagca caaatcgaaa aacggccaga ttcgctttgg cacgctttga
    52741 tcgattcatt attacagcaa aatttcaatc aaagcatgat gatcaccaag cagatgttta
    52801 tgaatggtca gacaattgac cagatcatgg caatccgtca tttaaaaaga ggaacagtaa
    52861 cagatcatct tattgagtgg gccttgtttt ttgatgattt tccatacgag tggattctct
    52921 ctgcagaaac aatcgaacgt ttggaaccaa ataaggattc cgtgcgagag tggcgcttta
    52981 gcgaatggaa tgtagatgga caattagatt atggagaatt tcgtttgtat caaatctatc
    53041 tattgagaaa ggaagcgata cagaatgtta ataaatgaat taaaaaaata ttttggattt
    53101 gattcatttc gtcccggcca agaagaaatt attcaggcgt tgcttgatgg agaagatact
    53161 ctcgctattt tgcctacagg aacgggaaaa agcctttgct atcaattgac ggggtattta
    53221 atggaaggtc tggtcatcat cgtgtcaccg ctgctttctc taatggaaga tcaagtgacg
    53281 caattacaaa aacgagggga gaaacgagta gctgctttca atagtttact ttcgcaatct
    53341 gaaagaagat acgtcctccg tcatctatcg caatataaat ttttgttttt gagtcccgaa
    53401 atgttgacta atccttctct tttagagcat ctgaaaaaac aaaatattgc gttatatgta
    53461 gtagatgagg ctcattgcgt gtctcaatgg ggagttgatt tccgtccaga atatcaacag
    53521 ttaggaaaaa tccgaaagct tttaggaaac ccggtcacac tggcactaac ggcgacagca
    53581 acagatttag tagcaagaga tatacgtcat gtactatttg atcgaccacc aaaagagatc
    53641 agacaatcag tcaatcggca aaatatctcg ctttttgttc ggaaaacgca gcaaaaagaa
    53701 caagaacttg aacacttcat ggaaagagca caaggagcgg caatcattta ttgtgcaaca
    53761 aaaaaagaag tcgaacgatt gtatcattta ttcagggaac gttttacagt tggatactat
    53821 cacggtggtc ttgatgcagc tcaaagaaga caacttcaac agcagtttgt aaaaaatcag
    53881 ctgcagtttc tcattgctac gaatgcattt ggtatgggga tcgataaatc tgatatccgt
    53941 tatgtggtcc attatgattt gccagacagt ctagaaaact atgtacaaga aatcggtcga
    54001 gcaggcagag atcaggaaga aagtgcagca attctttttt atcaaagtgg ggatgaacgt
    54061 atccattatt tctttaatca actttctaga gaacagcgac aaagttttga gctttatttg
    54121 gaatatgcca cagagcaagc tccctttgat gagctgcaaa aaaaatggat ggagctgatc
    54181 cagcagtctg aaaaaccaga aaattgggtg gaacggctca aacgtcagga aaaagaaaaa
    54241 gaattccgcc tgcagcagat gcttcgctat attaatgaag aaaactgccg cagaaaattc
    54301 atcttagctt attttggcga aaaactctcc gaaaagccgc agaattgttg tgatattgat
    54361 ggagcaaaag cagaacttct gccaaagagg aagctttttt ctaagactga acaacttcat
    54421 tgggaaacga ttttactaaa tttatttaaa aaaaacatgg aacattgatt ttttaggaca
    54481 agacactttt tcctatggta taatacacac gatagagttt aggaggtaaa acagtgagca
    54541 gaaaagatag acatcaatct acagaaaata acgaaacaca agaaccatgg gaacaaccaa
    54601 tctacgatac agatgatgaa gtttcatcaa gaagttctca gagacaacaa aagaaaggaa
    54661 atacattatt tctttcgcta tttttgattt tactggtctt atgtatcgct attccagcag
    54721 gtgcttattt ctggatcaga aatggatcaa gcaacaatgc aacagcagca acttcttctg
    54781 aaacttcgac atcagtagta gaaagttcta caagtacaac aaaagaggag tcaactgaga
    54841 cttcaacagt agaatctagt acaatcgcgg aagaatctac tgtgtctcaa gatacaggtg
    54901 taacgtcaga aagcagtatt gcagcagaga caacaccgac ttcctcaagt gaggcaactt
    54961 atcaagcagg ggattcaagc agcaatgcag cagaaacaac accttcttca tcagctgcag
    55021 ccggttcaac aacgactgtt caagcaggcg aaggtccaaa acaagtggct gagcgtgcag
    55081 ggatcaccac tgatcaattg tttgaattga atggcttaga tccaaacaac tttatgcttt
    55141 accctggtca agaattaaga attaaataat aatagccgcg ctttgcggct ttttttctga
    55201 gaaaaaggag ttatatcatg acacaaatca gtattgcaat cgacgggcct gcttcatctg
    55261 ggaaaagtac cgttgccaag atattagcaa aagaattgaa ttatatttat acagataccg
    55321 gtgccatgta tcgcgcagta acttatttag ctattacgaa caatgtatct ttttcagatg
    55381 aagaagggct agtttcgctg atcaaaaagt atcctatctc tttttcgcaa caagaagatg
    55441 gacagcatgt attcatagca tcaaaagatg tgactttaga aatccgtcaa ccagatgtca
    55501 caaaagctgt ttctgaagtc tctgcacatg gaaaagtcag agaagaatta gttgctgtcc
    55561 aacgaaaaat aggggaacgc ggtggcgttg tcatggacgg aagagacatc gggacagctg
    55621 tattaccgaa tgcagaagtg aaaatctttt tagtagcaag cgtaagtgag cgtgcagaaa
    55681 gacgtttcaa agagaaccaa gaaaaaggaa ttccgactga tcttgaaaca ctgacgaaag
    55741 agatacagga aagagaccat tatgattcaa caagggaagt ctctccgctg aagcaagcag
    55801 aggatgctgt tcgtatcgat acgactggca aatcgattcc agaagtcgtt gcagcaataa
    55861 aagctgttgt tttgaaaaaa ggttatcttc ttttttaaat taagaaaaaa aatgaaaaac
    55921 aattgttttt tcaaggaaaa cgaacgaaat cgctttattt tttttgaata atcaattaaa
    55981 ctaaaaacag taccgtactt acggcagaat gttggttagg aggacaagtg ttatgacaga
    56041 atttgatcaa aatcaagaga acaaccagtc aatggaagac gcaatgaaaa gcgtacaaga
    56101 agtaaaagtt ggcgatgttg ttaagggcga agttttagtc attgaagaca aacaagtgat
    56161 tgtcggaatc gaaggggctg gagttgaagg agtcgttccg gcaaaagaat tatcaacttt
    56221 gccggtagaa gatatcagcg aagtagtaaa agtcggtgat gtattagatt tagtcgttat
    56281 cagtacgatc ggcaaagata aagaaaacgg cagctatcta ctttcaaaac gccgtttaga
    56341 tgctaaaaaa gtttgggaag atatcgaaag agatttccaa gagggcaagg tcatcgaagc
    56401 acctgttaca aatgttgtca aaggcggttt agtcgttgac gtcggtgtac gtggttttgt
    56461 ccctgcatca atggtggaag atcattttgt tgcagacttt tcagactata aaggcaaaac
    56521 attgagcttt aaaatcatcg aaatcgaacc atcagaaaat cgtttgatct tatcgcataa
    56581 agcggtagtg gaaaacgaaa gagaagaaaa gaaaaaagaa attctttcaa ctatccatga
    56641 gggagatgtt ctcagtggac gtgttgcacg tcttactgat tttggcgcat ttgtcgacct
    56701 aggcggaatt gacggtctag tccatgtctc agagatttca catagtcatg tagcaaaacc
    56761 aagtgatgta ttggctgtca atgatgatgt aaatgtcaaa gtgttgtcta tcaatcctga
    56821 acaagaacgt gtttccttgt caatcaaaga cacacagcct ggaccttggt cagaaatcga
    56881 tacaaaagct ccagttggca cagtattaga cggaacagtt aaacgtttga caagcttcgg
    56941 tgcctttgtt gaagtgttcc ctggagtaga agggctagtc catatctctc aaatttcaca
    57001 taaacatatt gcgacaccac atgaagtgct tcatgaaggt gacgaagtga aagtgaaagt
    57061 attagagatc catccagaag aacatcgtat tgcattgagt atcaaagctt tagaagaaaa
    57121 accagcttca gaagaagaac caaaaaatgt tgagtcttat gaattaccag aagaaaatac
    57181 tggatttact atgggagaca ttttaggtga tgcgttgaaa gaagaagtag aagaaaaagc
    57241 aaccgatgat tctgaaaaat aattgatttt gtatctgtga ctgagatgaa agcagctgct
    57301 tttgtctcag tttttctttt gaaaagcgat tagtatttca ataaggaata aaaatacttc
    57361 gtttaattga caatctatgc ttgaaagttg gcaatgcatt cgttaaacta agtgagattg
    57421 gaaacttgtg aggaggaaat aatatggcaa atccaacaat tgcaatcgtt ggccgcccga
    57481 acgtcggtaa gtctacgatt tttaaccgca tcgctggtga gcgtatctcg attgtcgaag
    57541 atactcctgg agtaactcgt gatcgtattt atgcaaaagg tgaatggtta ggtcgagaat
    57601 tcagcgtgat cgacacaggc ggaattgatc ttggcgatga accttttatg gatcagatca
    57661 agcatcaagc agaaatcgcg attgaagaag cagatgtaat catttgtgta gtcagcggac
    57721 gcgaaggcgt gacagacgca gatgagatgg ttgcgaaaat tttatacaga agtaataaac
    57781 cagtgatttt agcagtcaat aaagtagata atccagaaat gagaaacgat atctatgaat
    57841 tttattcctt agggctaggc gatccttatc ctgtttcagg aagccatgga ctagggattg
    57901 gtgatatttt agatgaagca gtgaagtatt tctctgaaga gactgaagaa gaggacgacg
    57961 acacgattaa gtttagtttg attggtcgcc caaatgttgg taaatcctct ttgatcaatg
    58021 cgattttagg agaagaacgt gtaattgttt cggatattga aggaacgacc cgagacgcaa
    58081 tcgacaccca cttcgtttct gaaagtggtc agaaattctt aatgatagat acagctggta
    58141 tgcgtaaacg cggaaaagta tacgaaaata ctgaaaaata cagcgtgatg cgtgcaatgc
    58201 gtgccattga ccgttctgat atcgttttga tggtactgaa tgctgaagaa gggattcggg
    58261 aacaagacaa gcgagtagcg ggttacgccc atgaagctgg tcgtggaatt attatcgtcg
    58321 tgaataaatg ggatcttgta aagaaagaaa ccaatacaat gcgtgatttc gaacaagaga
    58381 ttcgtgatga attccggtat cttgattacg ctccaatcgt ttttgtttct gcagtgacta
    58441 aacaacggtt ggaacgtctg cctgaaatga tcgaacaagt aagtatgaac cagaatcttc
    58501 gtatctcttc ggctgtgttg aatgatatca ttatggatgc tgtagcaatc aatccaacac
    58561 caacagataa aggaaaacgc ctgaagattt tttatgctac acaagtcgct gtcaaaccac
    58621 ctacatttgt cgtttttgtc aatgaagagg aattgatgca tttttcttat gaacgtttct
    58681 tagaaaatca aatcagaaaa gcatttactt ttgaagggac gtccatccga attatccctc
    58741 gtaggagaaa ataggcattt taccatatcg acgttttttt ttcaaaagaa aaagacagtt
    58801 tcgtttgtta cgtttctatg actaaaaaaa gcatgtgaaa attggtaaaa accttgctat
    58861 taagcggttc atatgatatc gtgataacag aatattgaag ttaatcaata tttcttttta
    58921 aacttctgga ccactcagaa agttttaact aaatgatatt tctatcatat ctcttgtgga
    58981 ggaggtgaat ctatccatgg caaacaaagc agaattaatc gaaaaagttg cttcagctac
    59041 tgatttaact aaaaaagatg caactgcagc agtagatgct gtattttcaa ctattcaaga
    59101 tgctttagct aacggcgaaa aagttcaatt aatcggtttt ggtaactttg aagttcgcga
    59161 acgcgcagct cgcaaaggtc gtaacccaca aactggtgaa gaaatcgaaa ttccagctag
    59221 caaagtacct gcattcaaac caggtaaagc attaaaagac gctgttaaat aagacagttg
    59281 aaaaagcgct tgtggctcta gggctacaag tgcttttctt tttatttcct agcagaaaag
    59341 gaataaataa gagacagaga ttccgaatcc gtatgtcata catagaaaac acatgtattt
    59401 tatttaattt ttatttattt gcaagttatg aggtgagctt tgctcttatc ttttctagaa
    59461 tttgcttact aatggtaaaa taaaaataag aagaggaggg atgcgtatgg aacttgatta
    59521 tcaagtagaa gatgcgttga cgaaagtgtt ttacggagaa acgctagtca gtcagctaag
    59581 agaactttcc attgatcagc caatcttatt tttaacgaat cagcgctatt atgatttgtt
    59641 tgcagataag atcaacccgt tatttgcaaa tcaagcaaac aatgactggt atatttgtgc
    59701 caacactcaa tgcaatcatt taacagaatt aaagaacctg cttgatttcg tcaaacgcta
    59761 tcccgaaaat caatccatgt ttatcatcgg atttggaaat gaagggatca tggatctggc
    59821 aggttttttt caaaaacata caacccttga ttcatcacta tggatgatcc ctgtatcgat
    59881 ccgttctcta agccgagcat taacaccgca gcggacgatt ttgcagctac caaatcgtgt
    59941 agtgctgcaa accatcaatc tgccagaacg catcgtttat gatcaaacaa tcagcgagcg
    60001 gcaggtggac ggaaagcaga ttgacttttt gacttttatc tgttgcggaa tgatttgtga
    60061 tcatcgtttt ttgcaaaatc tgtataaaaa ctatccaaat aaaaagcaat taacgactcg
    60121 gccgtttgcc gggatgatcg aagaaatgat ctatttttat caattagagg cagaaaacct
    60181 gtcaaattat ggtaaactgt ttgaacaagc tttttatctg accgaaaatg gccatctact
    60241 ttctgctagt atgaaaaaat ttttaggaat gatgtttcat ttcgtgtgga atctccaagc
    60301 atatcctttt gatttccaac taaaaaactt ttttgtgtgg ctggacttgc ttggctatcc
    60361 aatccactgg ctggaacaga tttcaaagat ggagtatttg gaaaaagttg gcggatttta
    60421 taattaactt agccatttgg aaataaaaaa acgccacgtg aattccatgt tatattgaag
    60481 ttaccacaac tctcaataga caaggatgaa ttcacatgac gcaaaaccat gataacacag
    60541 tttcacgcaa gggaaaacac ctaacctact cagaacgttg ccaaatcgcc atcctgaaaa
    60601 aggaaaacta ttccaaccgt caggttgcgg atgtcctggg ctgcgtgccg cagaccataa
    60661 acaacgaaat caaacgcggg accgttactc agcttaaccg ccagaatcag aacgggaagg
    60721 tctacgacta ttatcatgac gtctacgacg ccgatgccgg gcaggcggcc tacgagaaga
    60781 accgcttgaa ctgcggcaga cggcccttat gggccgacac ggacgccttc attgaatggg
    60841 cggacgacat gatgctgttg gaaaaatggt ccccggacgt ggtggtgggc tttgccagaa
    60901 aacacgaatt gttcgacccg gccattatcc ctgggaccac caccctctat cagtggatcg
    60961 accgtggcat catgaaaacg aagaatatgg acctgctaga aaaactgtcc cgcaaaccga
    61021 aatcgtcccg tcatggcaac cgcccgaata aacgggtgct agggaaatcc attgaagagc
    61081 gcccggaggc ggttggtacc cggaaaacct tcggccattg ggaagtcgac acggtcgtag
    61141 gaaacaagac caagtcggat gccgtgctgc tgacgttggt ggaacggcag acacgcttcg
    61201 aggtcattct gaagctggcc gggaaggatg ccgaatcggt caaccaagcg gtccgacagc
    61261 tgagagaacg ggctggtgcg tacttcggcc agctgttcaa gacggttacc tcggacaacg
    61321 gctcggagtt cgcgggactg cacgatgcct tgcaggatgt ggcaaacatc tacttcagcc
    61381 acccctatgc ttcctgggag cgcgggacga gcgaaaacca acacaagctg attcgccgtt
    61441 tcatccccaa gggcagccca atcggcaacg tgagcgaaaa ccaaatcttg cggattcagc
    61501 gttggatgaa cgattacccg cgaaagattt tgggctatgc aacgccgcat gacacgttcg
    61561 tccaggcatt taggcaggaa cggttgacgg cttgacacgt gggcccttga caacgagcct
    61621 ccctcggcat ggcttgtgtc tggaaagctg gccggggcca gccagtcggg agactaccat
    61681 gcctcagctc attgtcaagg gcccactcga ttagaaaaat tagacggttg gtggtaacga
    61741 gcaaccgggt caccatgggt ggct
//
LOCUS       DACKAC010000007        61728 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.007, whole genome shotgun sequence.
ACCESSION   DACKAC010000007 DACKAC010000000
VERSION     DACKAC010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61728
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.007"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(58..186)
                     /locus_tag="I0B46_000464"
     CDS             complement(58..186)
                     /locus_tag="I0B46_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAR2082792.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(189..1562)
                     /gene="rlmD"
                     /locus_tag="I0B46_000465"
     CDS             complement(189..1562)
                     /gene="rlmD"
                     /locus_tag="I0B46_000465"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAR2082793.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1673..2728)
                     /locus_tag="I0B46_000466"
     CDS             complement(1673..2728)
                     /locus_tag="I0B46_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAR2082794.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2738..4168)
                     /gene="gatB"
                     /locus_tag="I0B46_000467"
     CDS             complement(2738..4168)
                     /gene="gatB"
                     /locus_tag="I0B46_000467"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAR2082795.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4165..5637)
                     /gene="gatA"
                     /locus_tag="I0B46_000468"
     CDS             complement(4165..5637)
                     /gene="gatA"
                     /locus_tag="I0B46_000468"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAR2082796.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5637..5942)
                     /gene="gatC"
                     /locus_tag="I0B46_000469"
     CDS             complement(5637..5942)
                     /gene="gatC"
                     /locus_tag="I0B46_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAR2082797.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6048..8084)
                     /gene="ligA"
                     /locus_tag="I0B46_000470"
     CDS             complement(6048..8084)
                     /gene="ligA"
                     /locus_tag="I0B46_000470"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAR2082798.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8101..10335)
                     /gene="pcrA"
                     /locus_tag="I0B46_000471"
     CDS             complement(8101..10335)
                     /gene="pcrA"
                     /locus_tag="I0B46_000471"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAR2082799.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10490..12049
                     /locus_tag="I0B46_000472"
     CDS             10490..12049
                     /locus_tag="I0B46_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAR2082800.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12160..14091)
                     /locus_tag="I0B46_000473"
     CDS             complement(12160..14091)
                     /locus_tag="I0B46_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAR2082801.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(14109..15023)
                     /gene="pfkB"
                     /locus_tag="I0B46_000474"
     CDS             complement(14109..15023)
                     /gene="pfkB"
                     /locus_tag="I0B46_000474"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAR2082802.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15020..15772)
                     /locus_tag="I0B46_000475"
     CDS             complement(15020..15772)
                     /locus_tag="I0B46_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAR2082803.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(15931..16140)
                     /locus_tag="I0B46_000476"
     CDS             complement(15931..16140)
                     /locus_tag="I0B46_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2082804.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(16151..17479)
                     /locus_tag="I0B46_000477"
     CDS             complement(16151..17479)
                     /locus_tag="I0B46_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2082805.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            complement(17844..18431)
                     /locus_tag="I0B46_000478"
     CDS             complement(17844..18431)
                     /locus_tag="I0B46_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAR2082806.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(18514..19764)
                     /gene="clpX"
                     /locus_tag="I0B46_000479"
     CDS             complement(18514..19764)
                     /gene="clpX"
                     /locus_tag="I0B46_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAR2082807.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(20011..21300)
                     /locus_tag="I0B46_000480"
     CDS             complement(20011..21300)
                     /locus_tag="I0B46_000480"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAR2082808.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(21454..22386)
                     /locus_tag="I0B46_000481"
     CDS             complement(21454..22386)
                     /locus_tag="I0B46_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2082809.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(22543..23202)
                     /locus_tag="I0B46_000482"
     CDS             complement(22543..23202)
                     /locus_tag="I0B46_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAR2082810.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(23205..24230)
                     /gene="sppA"
                     /locus_tag="I0B46_000483"
     CDS             complement(23205..24230)
                     /gene="sppA"
                     /locus_tag="I0B46_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAR2082811.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(24243..24533)
                     /locus_tag="I0B46_000484"
     CDS             complement(24243..24533)
                     /locus_tag="I0B46_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2082812.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            24943..26748
                     /gene="glmS"
                     /locus_tag="I0B46_000485"
     CDS             24943..26748
                     /gene="glmS"
                     /locus_tag="I0B46_000485"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAR2082813.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(26820..28175)
                     /locus_tag="I0B46_000486"
     CDS             complement(26820..28175)
                     /locus_tag="I0B46_000486"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAR2082814.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(28199..29368)
                     /locus_tag="I0B46_000487"
     CDS             complement(28199..29368)
                     /locus_tag="I0B46_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2082815.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(29365..30252)
                     /locus_tag="I0B46_000488"
     CDS             complement(29365..30252)
                     /locus_tag="I0B46_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAR2082816.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(30441..34121)
                     /gene="nifJ"
                     /locus_tag="I0B46_000489"
     CDS             complement(30441..34121)
                     /gene="nifJ"
                     /locus_tag="I0B46_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAR2082817.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            34319..34840
                     /locus_tag="I0B46_000490"
     CDS             34319..34840
                     /locus_tag="I0B46_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAR2082818.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(35539..36104)
                     /locus_tag="I0B46_000491"
                     /pseudo
     CDS             complement(35539..36104)
                     /locus_tag="I0B46_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(36283..36939)
                     /locus_tag="I0B46_000492"
     CDS             complement(36283..36939)
                     /locus_tag="I0B46_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2082819.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            complement(37507..38442)
                     /gene="whiA"
                     /locus_tag="I0B46_000493"
     CDS             complement(37507..38442)
                     /gene="whiA"
                     /locus_tag="I0B46_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAR2082820.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(38476..39474)
                     /locus_tag="I0B46_000494"
     CDS             complement(38476..39474)
                     /locus_tag="I0B46_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAR2082821.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(39471..40355)
                     /gene="rapZ"
                     /locus_tag="I0B46_000495"
     CDS             complement(39471..40355)
                     /gene="rapZ"
                     /locus_tag="I0B46_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAR2082822.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(40490..41221)
                     /locus_tag="I0B46_000496"
     CDS             complement(40490..41221)
                     /locus_tag="I0B46_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAR2082823.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            complement(41225..42490)
                     /locus_tag="I0B46_000497"
     CDS             complement(41225..42490)
                     /locus_tag="I0B46_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAR2082824.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(42753..45569)
                     /gene="uvrA"
                     /locus_tag="I0B46_000498"
     CDS             complement(42753..45569)
                     /gene="uvrA"
                     /locus_tag="I0B46_000498"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAR2082825.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(45579..47573)
                     /gene="uvrB"
                     /locus_tag="I0B46_000499"
     CDS             complement(45579..47573)
                     /gene="uvrB"
                     /locus_tag="I0B46_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAR2082826.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            47832..49334
                     /locus_tag="I0B46_000500"
     CDS             47832..49334
                     /locus_tag="I0B46_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAR2082827.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            49336..50073
                     /locus_tag="I0B46_000501"
     CDS             49336..50073
                     /locus_tag="I0B46_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAR2082828.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(50441..51607)
                     /gene="dnaJ"
                     /locus_tag="I0B46_000502"
     CDS             complement(50441..51607)
                     /gene="dnaJ"
                     /locus_tag="I0B46_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAR2082829.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(51758..53587)
                     /gene="dnaK"
                     /locus_tag="I0B46_000503"
     CDS             complement(51758..53587)
                     /gene="dnaK"
                     /locus_tag="I0B46_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAR2082830.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(53638..54201)
                     /gene="grpE"
                     /locus_tag="I0B46_000504"
     CDS             complement(53638..54201)
                     /gene="grpE"
                     /locus_tag="I0B46_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAR2082831.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(54295..55338)
                     /gene="hrcA"
                     /locus_tag="I0B46_000505"
     CDS             complement(54295..55338)
                     /gene="hrcA"
                     /locus_tag="I0B46_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAR2082832.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(55573..56739)
                     /locus_tag="I0B46_000506"
     CDS             complement(55573..56739)
                     /locus_tag="I0B46_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAR2082833.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            complement(56755..56982)
                     /locus_tag="I0B46_000507"
     CDS             complement(56755..56982)
                     /locus_tag="I0B46_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAR2082834.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            complement(57369..57625)
                     /locus_tag="I0B46_000508"
                     /pseudo
     CDS             complement(57369..57625)
                     /locus_tag="I0B46_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            complement(57968..58991)
                     /locus_tag="I0B46_000509"
                     /pseudo
     CDS             complement(57968..58991)
                     /locus_tag="I0B46_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            complement(59629..60303)
                     /locus_tag="I0B46_000510"
                     /pseudo
     CDS             complement(59629..60303)
                     /locus_tag="I0B46_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313658.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            60826..61416
                     /locus_tag="I0B46_000511"
     CDS             60826..61416
                     /locus_tag="I0B46_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAR2082835.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQKEGWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKKIRLYKLGFYDYYKKYGDPFS"
BASE COUNT        17125 a        13257 c        10689 g        20657 t
ORIGIN      
        1 aaaaagacta agaaacaata acaaaagtta acacttctta gtctttcttt agcacgtcta
       61 tttattaaag attttagggc cttttctcat gtttttacga tcattcttag gtgtttccat
      121 ttgtttggat cggcctccac cttgtttctt tttgatcaat tctttcattt tttctttttg
      181 gtccattttt attctccttt aacgaatgaa gcgactaatt cgatatgatg ggtttgcggg
      241 aaaagatcta caggctgaac tttcttcacg gcataacctg cttctgcaaa taacttagca
      301 tctcttgcaa aagttgccgg attacatgag atatagacga ttcgttctgg tgacatttcg
      361 attgctgatt caataaaatg atgatctaat ccttttcgtg gagggtcaac tacgatcaca
      421 gatggtttga tccctttttc ttgccatttt ttcataactt tttctgcttt tccgacttca
      481 taatgaacat tttcgatttt attatttaaa gcattgaact gtgcatcttc aattgcttct
      541 ggaataactt ccatcccata caccttatcc actctgtctg ctaaagacaa gccaatcgta
      601 cctattcctg aataagcgtc gatcacaatg tcttttttat ctaactcggc aaactcgatg
      661 gctgtttggt acagatactc tgcttgttga gtatttactt gataaaatga ctttgctgag
      721 attcgatagg tttttcctaa tagctgatct tcgatatagt ctctgccata taaaactttt
      781 tctttgtctc ctaaaatcac atttgttttt tcttcattga tattttggat gaccgaaacc
      841 acttctggca attcttcatg aatagcttca gcaatttcct tggatttaaa gaatttttct
      901 ttacgagtaa ctaaaacgac catcatttca tgggaataat gaccacgacg gataatgatg
      961 tgacgaagct gtccttcatt tttttcttca ttatatcctc gtacttggaa ccgttgcaag
     1021 atatctctga ctactttgat tgcttgatcg atgacaggat cttggatata aaaatcttcg
     1081 atagggacta gatcatggct attctttttg taaaaaccgg tggctaactg tccatcgata
     1141 cggcgaacag ggatctgtgc tttattacgg tagccgaatg gttctgccat tccgaccgtt
     1201 ggaagtacag gaacttctgg catcttagca atcttcgcca ttacatgctc tacttgtttt
     1261 tgtttgaaga caagctgttg ttcatacttc aaatgggcta aaggtgcgat acctatgcga
     1321 agtaatgcat tgttttctac aggctgacga tcaggtgatt gtgtaataaa tgtttcgact
     1381 tttccatatc caaacttatt gcctgctttt actactagta tttctacttc ttctccaggg
     1441 agtgcatttt cgataaataa tggataacca tcgatttttg ctacccccat tccttcataa
     1501 ctcaagtcga cgatttcgac tgtatatcgt tcgtttttct ttactatttg ctgttctgtc
     1561 atattttgct cctttaagcc tatttttcaa tcctactgaa ctctactata aatcagctaa
     1621 ttacacaact aaaaccggca ctgaccttac tacgttcagc accggctttt gattaattct
     1681 tttgattagg tgtttcgtct gctatttttc cgtcaccgtc gatatcttca tccgttaaac
     1741 gttcgacttc ttttacgaat tctttgctga caacctctaa ttcttcttca tctgtacctg
     1801 tgattgcttc atcaggaatc tgatcggtat tggcaaagaa ctcaatgtgt tgatgaagat
     1861 tttggaaatg catcggtgct tccccgccgt attcaccatc taagttgatc attactggtc
     1921 gattgatatc atcttctgtt tcaacgaaca aactgcttgt tttggtatag attattcttg
     1981 gatcatcgac atgtcgaccg ccattcagca tgagtgctgc aatatgcaag atctcaaaga
     2041 tatttgctgt cttcacaatg attaaagaaa acttcccatc atccaatttg gcatctggcg
     2101 cgatctgttc gaatcctcca acagagttcg tcagccctag gaaaaacatc gaagcatttc
     2161 cgatatattc cccttcatca tacttcaagc gcattttgat tggctttact cgagggagca
     2221 tctctgctcc tttcgcaaga taagcaagat aaccaaaaat actctttagt tctgatggaa
     2281 cttcataagt caattctgtc aaatagccac ctgcagcgat attgatgaag taactgttat
     2341 cagaaaggcc gatgtccatt tttacggttt ggtgtttttt tatcacttct gctgctgctt
     2401 taatattatc tctaggaatt tttaatgctc gagcatagtc gttcgttgta ccagctggga
     2461 tgatggccat tttaggacgg tgttcaagcg gagcgattcc attaaccact tcgttgattg
     2521 tgccatcgcc accagctgcc actactagat caaaacctag ttctgcgact ctacatgctt
     2581 cattcttggc tgaatctggt tctggtgtag ttgcataggc gctggcttca aatcctgctt
     2641 cttctaatgc ctgtaggata tccgccaaat tttttttcaa taattctttt cccgaggtgg
     2701 ggttataaat tacgcgtgct tttttcattt tcagcctcta tcgtttcgct aattcatctt
     2761 taagtagctt attcacgaca ccaggatttg cttgtccttt agttgctttc atgatttgac
     2821 cgactaggaa gccaaccgca cgatcttttc catttttgaa atcatcgaca gattgttggt
     2881 tgttatccaa aatttcatta atgattggca gtagtttgtt cggatcagat aattgcaccc
     2941 aaccattttt ttcaactact tcttgggctt ctccgccatt tgtgatcaat tcacggaaga
     3001 cttttttggc gatttttgag ctgatcgttc catcggcgat caaacgaatc attcctgcta
     3061 agttctcagg tgtcaatttt gtctcagcta attccagttt ctcactgttc aagtaagcag
     3121 aaacttcacc catcaaccag ttagatgctt gcttggcatc tgcaccattt tccaaaacag
     3181 cttcaaaaaa atcagacatt tcttttgtaa gtgtcaatac cattgcgtca tactctggaa
     3241 gtccaaactc acggatatag cgttcgcgac gagaagctgg catctcaggc aaactagctt
     3301 ttactttttg aatccattcg tcatcgatca cgaatttcgg gatatcaggc tctgggaagt
     3361 aacgataatc actagaccct tctttgacac gcatcaaaac tgttttattc gttgtttcgt
     3421 caaaacggcg tgtttcttgt tggatttctc ctcctgaaag aagaactttt gcttgacgtt
     3481 tttcttcaaa agctaaacct tttttcacga aagataaaga gttcaagttt ttcaattctg
     3541 ctttcgtacc gaactcttct tgtccataag ggcgaagaga aatattggca tcacagcgca
     3601 tagagccttc ttccattttc acatcgctga cttctgtaaa ttggataatt gatcgcaatg
     3661 cttctagata agcgtatgct tcttctggtg aacgcatatc agcttcagaa acgatttcga
     3721 tcaaaggcgt gccttgtcgg ttcaaatcaa cgtaagaata accgccaata ccatgcatgt
     3781 tttttccagc atcttcttct aaatggacac gttcgatccg gatttttttc tttttacctt
     3841 ctacttcgat ttcgatccat ccgtcatgac cgattggttg atcaaattgg gaaatttgat
     3901 atgcttttgg attatctggg taaaagtaat ttttccggtc aaaatgagta tctttagaga
     3961 tttcacagtt taatgccaaa gctgctttca tcccgaattc cagcgcagct ttattcatta
     4021 ctggcagcac gcctggataa ccccagtcga tcacgtttgt attgctgttt ggttctgcac
     4081 caaaatgagc tggcgctgga gagaaaattt ttgagttggt ttttaactcg acgtggactt
     4141 ctagtccaat gactgtttca aaattcattc gtctttccct cctaaaatag ctggttgttg
     4201 tttatggaat tctgttgctt gttcaaacgc atatgccgct tgatacatcg ttttttcatc
     4261 aaaataatta ccgatgatct gcaatccaac tggtaatcct tctgataaac ctgccggtac
     4321 agacatacct ggaagtccag ccaagttgac agggatcgtt aagatgtcac tcatgtacat
     4381 tgtgattgga tcgttaatgt tctcaccgat tccgaatgca actgttggtg aagatggccc
     4441 gatgatcaag tcataatctg caaagacttt ttcaaaatct cgtttgatca atgtacggac
     4501 ttgtcccgct tttttgaagt aagcgtcata ataaccagca cttaaggaga atgtacctaa
     4561 catgatacgg cgttttactt cttcaccgaa tccttctgaa cgtgagttga catatacatc
     4621 atctaatgat ttcacatttt ctgagcgata accgtagcgg ataccatcga atcgttgcaa
     4681 gttggaactt gcttctgatg aggcaatgat ataataaaca gccacaccgt attttgaatg
     4741 aggcaagctg acttcttcca caacagcacc taatgaacgg aatgtttctg ctgctttcaa
     4801 tacagcttct tttacgccag gggccacacc ttcgcctaag tattctttag gtaaagcaat
     4861 cttcattcct ttgatgtcgc ctgtcagtcc ctctgcaaaa tcaggaactg aaacgcctga
     4921 agaagtaccg tctttttcgt cataaccgct gatagctgtc aatgctaatg cattatcttt
     4981 caccgtacgg gtcattggtc cgatttggtc aagagatgaa gcaaaggcaa tcaaaccaaa
     5041 acgtgagaca cggccataag taggtttcat ccctacgatg ccgttaaaag ctgctggttg
     5101 gcggatactt cccccagtat cactacctaa agaaactgga atttgtccag ctgcaactgc
     5161 tgctgcagaa ccaccagaag agccaccagg tacttttgtt gggtcccaag cattttttgt
     5221 tttcttgaaa taagaagttt ctgtacttcc acccatagca aattcatcca tgtttagctt
     5281 accaacaggg atcatatctg cttgatagat tttatccatg accgttgcat cataaattgg
     5341 ttcaaaattg taaaggatct tagatgcagc ggttgtcaag atatcttttg ttacgatatt
     5401 atccttgata ccaattggaa taccagcaag gacgttgctt tcatcaatcc ctttttcatc
     5461 taaagctttt gcctgttcca atgctttttc ttcgttcaat gtaatgaatg cttcgacctc
     5521 aggctctgtt tctttgattc gttcaaatgt tgcttttgtc aaatcttgta cagtgatttc
     5581 tttcgataca agcatatgat gcagttcttc tagagaacga ttgtgtaatt ctgtcattat
     5641 gcgccagcct ccccattatc gatgattgcg ggtactttga taaagccgtc ttctgtttcc
     5701 ggcacatttt tcaataattc atcgcggttc catccagctt gcgcccgatc ttgtcgcatc
     5761 acgtttactg tttcaaccac attcgacgta aatggaacac cttctgtatc tacttcttcc
     5821 agcatttcaa ccatatcgat gattttacct aattggtcgg taaaacttgc aagttcttca
     5881 tctgcgaacg caagttttgc taatttggca acgtgtttca cttgttcttc actaatcgcc
     5941 atactacccc ttctttctaa acgagttaaa aactttgctc tctattttaa cacaagagtg
     6001 ccgtgtctga aattgtttat tgaaatttgt ctgaaaaaac tcatttttca ttcttctact
     6061 gagcgacttt tagccaatgc atctgccatt tgatcttctg tccaaacttc gattcctaat
     6121 tcttgtgctt tggtcagctt gctgccagca tcttctcctg ctaccacgat atccgttttt
     6181 ttcgatacgc taccagtcac tttcccgcca agattttcga tcgtctcttt tgcttcttca
     6241 cgagtaaatc gtgttagttt accagtaagg actactgttt tttctttaaa aggagactcg
     6301 acttcttgca attgggcatt tcgaagccct ttgtattcga aattgacgcc tgcttgtttt
     6361 aattcattca tcaattcatg gacttcttcg ttactgaaat aagtgactac actgtcggca
     6421 atgatatcac cgatcgtatc aagcgcaata atcgactcat agtcactttt actcaatgtt
     6481 tctagatcac cgaaatgttc tgctaaaatt tttgctgctt tcgctccgac atgtcggatt
     6541 cctaacccga aaatcaaccg ctcgaccgaa ttatctttgc tgttgtcgat tgctgtcaaa
     6601 atattatttg ctgatttttc ttttattttg tccaacgtca ataactcttc ttctgtcaat
     6661 ttgtaaagat cagcaacatc tgctactagt tttttatcat acatctgttc tagtacgcgc
     6721 ggccccaacc catcgatatt catcgcattt cttgaaacaa agtggttcaa accttctttc
     6781 atttgagcag ggcatttagg attgatgcac cgtaaggcta cttcttcatc caaatggaca
     6841 agttcacttc cacaaactgg acaatgtgta ggcaattggt attcttcgct gtctttaggt
     6901 cgtttatcta acacgacttg cgaaacttct gggatgatgt ctcccgcttt ataaatcaga
     6961 actgtatctt tcaagcggat atccttcaac ttaatataat caccgttatg caggctggca
     7021 cggcttactg tcgttcctgc tactcggact ggtgtcatga tagctgttgg cgttactacc
     7081 cctgttcgac cgactgtcca ttcgatgttt tcgattagcg tttcaacttc ttctggcgga
     7141 aacttgtaag ctgctgccca tctcggtgct ttgacagtaa aaccaagttg atcttgtagc
     7201 gagaattcat tcactttgat gacgattcca tcgatttcat agggcaaatc cacacgtttg
     7261 tcatgatatt cttcaatgta tgaccacact tcatcaatcg aatgacatag acgtttttcc
     7321 cgattcgtgt ggaatccaat tttttctagc tcttctagcg catcatactg ggtttttgct
     7381 tgcataggac cgaaatcagc tactgtgtat aaaaaagtgt ccagatttct tttcgctgtg
     7441 attttagaat ctaactgacg caaagaacca gctgcggcgt tccgaggatt cgcaaagata
     7501 tcttttccct ctgcttctct gtcttggttc aattggacaa atgaccgttt tggcatgaaa
     7561 cattcgcctc ttacttcgat gttcattggt tcttttaatt tgattgggac cgaccggaca
     7621 gtttttaggt tttcagtgat gttttctcct acagttccat ccccgcgagt cgcacctctc
     7681 acgaaatttc catcttcata acgcaaagaa acggaaagtc catctatttt tagttcacag
     7741 caatagtcaa ctacacgtcc cactgctttt tgtacacgtt gatcaaaagc tatcagctct
     7801 tctttgctga aaacgtcgtt caaactgtaa agcggaatat catgagtgac tttttcaaaa
     7861 ccttctaata cttttccgcc tactcgttga gtgggagaat cagacgtgat caaatcagga
     7921 tattgttcct caattgctac taattctgcg tattctttat cataaacata gtcttccact
     7981 gttggtttat cttctacgta gtattcgcga gaccattggt tcagtcttgt acgaagttct
     8041 tctgctcgtt tagcagcttc gtcaatcgtc atttcatgtt ccatcattta ccctccacta
     8101 ctaagctttt tcaattggcg caaaggcagc caaaagacgt tttactcctt gacttgggaa
     8161 agccacatct agttctagat ccttcgacgt cccgcttact cggacgactg ttcctagacc
     8221 ccattttttg tgtttgactt tatctccagg tttccagctg ttattttccc ctccagtagc
     8281 acttttagag ggaacaaccg ctgtctctgg ctgtttatag ctatatgctg tttttttagc
     8341 gaatacaggt gtttttttag gcgtaggacg catgccttcg atttccagta actcctgatc
     8401 gatttcttct acaaaacgag aaggtcggtt atattgcgtc cgtccatata atgttctgga
     8461 gaaagcattt gttaaataca acgcttcttc tgctcgcgtg atccccacat atgcgagacg
     8521 gcgttcttct tccagttcgc tttcttccat caatgctcga gataaaggaa aaaccccctc
     8581 ttctagacca atcaggaata caactgggaa ttctaatcct ttggctgcat gcaaagtcat
     8641 caatgtcact tgggaagcat cttcttctaa attgtcaata tcggaaacaa gtgccagatc
     8701 atttaagaag accgttagtt tttcttctgg tgcatcagcg tcttcttcat tttgctgttc
     8761 gaattgtttg tcgaattctt gtgtgaccgt taaaaattcc tccaagtttt cgatgcgtgc
     8821 ttgtgcttct aatgtatttt ggatttttaa gtcttctaga tatccgctac gatccaaaac
     8881 ctctttcgtc aattctgtca ctgtcaagta tgggatcatc tgagtgactt cttggatcat
     8941 ctctccaaat gcacctaatt gctgtcctgc ttttccaccg atatttgcca gatccacatt
     9001 ttgcgctgct tcaagcagtg gccattcatg aagagaagca aaactgcgta gtttttcgat
     9061 agatcctggt ccgatgcctc gtttgggcga attaacgata cgctcaaaac tgattgaatc
     9121 ttgaggattt gccagcacat tcaaatacgc taaaatatct ttgatttctt tacgatcgta
     9181 gaacttgtgc cctccaacca ttttataagg aatatttgct ttcaacagag tttcttccat
     9241 tacacgagat tgtgcgttcg tccggtacaa gatggcaaag tcgccgtaat tccgatgatt
     9301 gcttcgaatc tcttcttgca tccgatctac gataaaacgg gtttcgtctc gttcattatc
     9361 tgcacggtag taagtgatct tgtttccttc tttgttttct gtccacaagt tttttggttt
     9421 tcgattgcta ttgttttcaa tgacttggtt tgctgcgcta aggatgtttt tcgtggagcg
     9481 atagttttgt tctagcaaaa ttactgcagc atctggataa tctttttcaa aatccaagat
     9541 gttctgcata tctgcaccgc gccagccgta aatactttga tctgcatctc ccacgacgca
     9601 caggttccta aatctacctg ccaataaatt gactagcgta tactgtgcgt gatttgtatc
     9661 ttggtactca tctacatgaa tataatggaa tttgttttga taatatgtga gtgaatcagg
     9721 atgttcttca aataatcgaa tcgtattcat gatcaggtcg tcaaagtcca tacattgatt
     9781 atattgaagc tctttttggt aagcagcata acattttgcc gcaatctctt cgtaataact
     9841 gccttgcatt tttgcaaaat cctgaggggt ctgcaggctg ttttttgctt gagagattgt
     9901 tcctaatata cttcgaggat catatttttt cggatcaatg ttcagttctt ttagtatccg
     9961 tttcatcaag gtcagctgtt cagaagaatc gatgattgta aagttccggt tatacccgat
    10021 aaaatcaaca tctcttctta aaatacgcac acacatcgag tgaaaagtcg atacccagac
    10081 atcttcgcct ccagatgcga gaatcgcatt gacacgttct ttcatttctt ttgcagcctt
    10141 attggtaaaa gtaatcgcta ggatgttcca aggattgact tctttttcct caatcaaata
    10201 ggcaatgcgg tgtgtcagca ctctcgtctt tccacttcct gcacctgcca ttagcagtaa
    10261 tggtccgtca gtgtgaagaa cagcttcttt ctgacggggg ttcatcccgt ttaataattc
    10321 agctttggat gacattaaca acatcctttc ttttatcaat cttgttaatt atagcatttt
    10381 cttggctttc attaaactag ctagttcaaa atcatttgtt tctttttatg gatttttcct
    10441 tgtcaattcg ttgatttcgc gctaaattaa aagaaagcga ggcgtttgaa tggaaaaaaa
    10501 aggaaaaatc aagaaattag ataagactca gatatatttt attctaaaag gaatatttgt
    10561 tggactgctg acaggaatcg tcgttagcct gtttcgatta tctatcgaaa agctaagcga
    10621 tttagttcga agtatctatg aaatgagtcg ggaacagccg atttacttaa ttggcctagc
    10681 tatttgctgc atccttgcag catttttcgt gggttatctg gtcaaaaatg aaccggatat
    10741 caagggaagc gggattccac aagtagaggg acagcttcgc ggggaattat caatgaactg
    10801 gttctctgtt ttatggaaaa agttcattgg cggtgtactt tcagttggtg cgggcctttt
    10861 tctaggtaga gaaggacctt ctatacagct tggcgcatcc attggacaag gagctggaca
    10921 attgtttcgg agtccatctt cagaagaaaa gattttaatt tctagcggtg caagtagcgg
    10981 acttgcagca gcattcaatg ctcctatcgc aggattactc tttgttttag aagaaatcca
    11041 tcactccttt tctccacttg tttggctgac atcatttgct tcagcaatca ctgctaactt
    11101 cgtctctctt tactttttcg gcttgagacc tgttttatat ctaggtgacc ttccttcttt
    11161 gccgttgaaa tattatggtt ccttagtgat cttaggagta cttctaggca tcttaggttt
    11221 tatttatcag aaggtcttgc tggctttgcc aaaattttac aaccgattgc ctttaccagc
    11281 ttattttcac gggttcgttc ctttcttgct gattttgcca attggttatt tatggcctag
    11341 tactctaggc ggaggcaatc aaatcgtttt agcttttgga catgcttcct tgccaatcat
    11401 tatgattatc ggtttgtttg ttcttcgatt cgttttttcc atgatttctt acggtggtgg
    11461 tcttccaggg gggattttcc ttccgatatt gacattgggg gctttaatcg gtagctttta
    11521 tggaaacatt atggttgatt tcgggatgga tccaatctac atcaaggatt ttgtcgttat
    11581 tgccatggct ggttatttca cagcgattgg aaaagcaccg ctgacagcaa ttattttagt
    11641 gacagaaatg gttggctctc ttaatcatct gatgccttta ggattggctg ctcttgttgc
    11701 ttatatcgtc aatgaccttc tcggcggtaa tccaatttac gaatcattat tggaacgtct
    11761 acttggaaat actcagaccg cctcactaaa aggtgcaagg acaacttttg attttccagt
    11821 aacggcagag agttctttag acggattaat ggtacgagac ttcaattggc ctaaaaatat
    11881 gctgctaatc tctatacgca gaggaaatca ggaaatcttg actcacggtg atacgatcat
    11941 gaatgtagga gatattttgg ttatcttgac tgatgaagca aacttgccaa gaatcaagca
    12001 ggaaatcgag gctaaatctt ctatcacaaa tttgaagcaa tcattttaac gaacatggaa
    12061 aaactatttt acaacaacta ttcatctaca ctacttgatt ctatatgatt ctataaaaaa
    12121 agaagagact gggaaaaacc agcctcttct tttttatttc tatgcttctg ctgctgcagg
    12181 agcttttctc aataaaccat aaatcacacc agatacgact gctccgatag caataaacac
    12241 caaatacatg accgggtgac tcagtaagaa tacaacgaaa atcccgccat gaggagccag
    12301 taatttgatc ccgaaagctc cgactaatcc gccagttaaa gcagaaccaa taataaaact
    12361 tggaatggca cgaatcgggt cagctgctgc aaatggaatc gatccttccg tgacaaacga
    12421 tagtcccatc acgatgttcg tcaaaccagc atcttgatct gttttgctga atttattctt
    12481 aaacaaacgt gtagcaacga aaattgccaa tggaggtacc attcctccag ccataacaga
    12541 agccatcacg acacttccgc cagttgcaac agtcgtagct aaagttgctg taccaaacac
    12601 ataagcggct ttgttgattg gaccgcctaa atctgccgcc atcattccag ctaataatgc
    12661 accaagtaaa gcagcattcg aaccactcat acttgtcaag aaaccgttca atgcatcatt
    12721 gattgctttc atagggacat tgaccagcaa catcaagaaa ccagtaatca gtaagccaaa
    12781 cactggataa aagaggatcg ttttaattcc atccaatgat tttggtaagt tcttgaataa
    12841 tttacgtaaa aatacgatga cataaccagc taagaatcca ccaatcagtg cacctaagaa
    12901 tcctgcaccg cctgtattag ctaatgcacc agcagcaaat ccaacgatca aaccagggcg
    12961 gtcaccgata cttgaagcaa taaatcctgc taataccggc agcatgaaac caaatgcagc
    13021 ttgtccgatt tggttgaacc agctagcagc ttgattgtaa ttccctagat ttgataattg
    13081 atcatgtggc acgccaataa attggtcgat catgaaagaa agcgcaatag cgattcctcc
    13141 accaataacg aaaggtaaca tgtgtgatac gccgttcatc aaatctttgt aaattctttg
    13201 tccgatagta ccatccgcat ttccttcttc tgaactgctt tcttttccat caccatggaa
    13261 tactggtgca gtaccactaa gggctagatt gatcagctct tctggtttac ggataccatc
    13321 actgacagga cgattgatca gttcttttcc gtcaaagcga ttcatctcta cttttttgtc
    13381 tgctgcaacg attacacctt ctgcacgggc gatgtcttca gcagtcaatc ggtttttgat
    13441 tccttctgaa ccgttcgttt cgactttgat ctccacaccc atttctttcg cttttttctt
    13501 cagagcatct tctgccatgt aagtgtgagc gatccctgtt ggacaagctg taacggcaac
    13561 aacgaatttt ctttctggat gagcagaagc ttctgcagct tgtgtttctt gttctgcttt
    13621 ttcttctgct tctttttgct cttcagctgt ttggaacaag tcttggactt cttctggtgt
    13681 atttgcttct tttaattttc ctacgaaatc tgcatcaatc agcaatcgag atagtgctgc
    13741 taatgcttgc aaatgtgtgt cgtttgcgcc ttctggagct gcgatcatga aaaacagata
    13801 agttggctgt ccgtctaagg cttcataatc tactcctttt tggctttttg cgaataaaac
    13861 tgttgcttct ttaactgcac tgtttttcgc gtgaggcatg gcgattccat caccaagacc
    13921 tgtagaagtt tgtgcttcgc gggctagaat tccttcttta tatgtttcga tatcggaaat
    13981 ccggccggca tcaaacattt tttggaccat ttcatcgatt gcgccttttt tatcagtcgc
    14041 ttgcaaatcc atgatcatcg cttctttgac cattaaatcc ttgatattca tgattatcct
    14101 ccttgaaact aatcaatttt tgtgatttca acttctggca gcaactcatc aatcaattcg
    14161 cgagctgcca agtcatcaga aaaagctgtt gcgcttccgc aagcaactcc ccatttaaag
    14221 gcttcgattg gatctttcgt acggctgtaa gccccaataa agccggcaat cattgaatcc
    14281 cctgcaccta ctgaattttt caatgggcga actaatacat tcgttttata agttccttgt
    14341 tccgtgaaga gcaaagcacc gtctccagcc attgagatca gcacattctg tgccccttca
    14401 gctagcaatt ttttaccata cggaatgatt tcttctacag attggaaggt tgtattgtaa
    14461 agctcagcta attcgtgatt gtttggtttg atcaacaatg gtttcttaac aagtgcattt
    14521 tttaggtcat cacctgttgt atcaatcacg aaatctgcac ctttttctcg aatgatatca
    14581 atcagttctt gataaaatcc ttttcgcagg ctagccggcg cgcttcctga caatacgacg
    14641 atatctcctt gtcctactgt tcctaatgct tgcttcaact catcgatttc ttcatctgaa
    14701 atagatgggc ccaaaccgtt aatttcagtt tcagtatctg attttagttt gatattgatt
    14761 cttgtatctt cttttacggt tgtgaacaaa gactggattt gttctttttc aagccacttc
    14821 gaaataaatt cgccagtgaa ccctcctaaa aaccctagtg ctgtagattg agaatcgatc
    14881 cgtttcaaaa tacgggaaac attgatccct tttcctccag gtaacttgaa gtcttccttc
    14941 attcggttca aatcgcctag ttttaaccca tctacgcgta cgatataatc aattgatgga
    15001 ttaagtgtta ctgtgtaaat cattgtattg cctccttaat tgttgtcttt tcacggaatt
    15061 cattaacgaa ttctaatgga caacgctcgg ttatgatcgt ggctgcttcc aaaggtgcaa
    15121 cagaggtgaa ggacaactgg ttgaatttgg tatgatcaat caatacaaat gtctcttcgg
    15181 aatttttcat ggctaaacgt ttaacagctg cctcttctgg gtccggagtc gtgaatcctt
    15241 gttcaagatg ggcaccgttc attcccataa atgctttatt aaaatgaaaa ttttttagtt
    15301 gttctatcgc attagatcct aaaacagcat tagttgccag ctttatcgtt ccacctaaaa
    15361 taatggtagc aagctccaaa tcaactaaat ttgccgcatg ttgaacggaa tttgtcacta
    15421 ctgtgatctg ttttccttgc agaaaaggaa tcatttctaa cgtagtagag ccagcgtcta
    15481 aataaatgac atcttcgtca ttaatggttt cagctgccga tttggcgatt tgctgttttt
    15541 cgtgaacgtt tttgagtgat ttttcactca tattctgctc aaaattcaat aattgctctt
    15601 tcttagctcc gccatgaact ctttctagaa acccactatt ctccagctct tgtaaatcac
    15661 gtcttatcgt tgattcagaa gcattaaaaa ggtttacaag atcttgtgat ttcacgattt
    15721 tctgctgttc aagcaagtct aggatttttt gtctacgttc ctctgtaagc attttcacga
    15781 tcacctcaat acaaataata gcatattttt tttctactcg caatataaat cagtcaaaaa
    15841 cgttcataaa aattataaat cagtcaaaaa aaaataaaaa accagtcaaa aagactggtt
    15901 ttttattttt ttgtctatca tcaagccgtt ttatcgtttt ttattttcgc ttcttgtttt
    15961 tttcatataa acagaagcag gttttctttc cttatgaaga agataataat aaagtcccat
    16021 cccgccaaac atgaccaaaa aaccgatgac tgatcgaata ggaaaattag gaacaaaaaa
    16081 gagacagacg gataataatg atgccggaac cattattcga taatagagtt gtttatccat
    16141 tttcttcacc tcatattttt tgtaaagtaa tcaattgttc taattctcgg taatctaaaa
    16201 attgcacacg cagatgctgc tcacctttct tgacatcgaa ttggatactt cgccgctttt
    16261 tctttattcg tttgaaggta acatcagaga gtctatgttt gatttccttt aacggtaatt
    16321 ctgacaggga ttctgaagga acatagattc ttaaggataa aagatactct tgatattttt
    16381 catctgttac ctctacttct agatattccc taatataata acaataagct tcgattccat
    16441 tatccaaagc aacagtcgga tcgataactt tatagcacat catgcgcgag cgattctcgt
    16501 caacccaagg aaaatacgcc tgagcattta gtgttccgcg aacacgcctt tctttataat
    16561 ctccttgttg aatatcataa ctttttgtta caaaaaatga agcattcttt ttaggaacag
    16621 gttgcatttg tgcattctga tactggacag attggatcgt atggtcagtg taaatgtatt
    16681 caacgaatgg cggtttcaat aaaccgacac cattgtccca agcaagatga tcaaacccaa
    16741 gctggtccct aaaataggat tgaaacattt gattgtttgg cttttgccag ttaaaggggt
    16801 ctcgataaag ctggatacat atgacttcgt tttctaattc cttcactttt tctacttgtt
    16861 ggatgtcgag aaaaatcggt ttagggatga tttgatgata atagcgacta taatacattt
    16921 cccaacacga agtgaaacat aaaccttggt aaaaaagatc gtatcctgga aattggttgc
    16981 aatcgactac tacttccccg ttttgttgtt tcattttagg taaatgttcg atttcatcct
    17041 ttttctcaaa caatagccgt tgagagatct gttttgtatt gtgatagata aactcgctat
    17101 aagctcgcat ataggcgaac accccatgag caatcatttt tttctgaatg aagtctcgaa
    17161 tcaaaaattg cttttctttg aaaacagaga tagacaatac attttttatc aataattgat
    17221 tatcttccaa gcgaaaacta cattcgttct cgtcatttcc tacaactaac caactttcgt
    17281 ctcttaccga tgctagcatt attttttctt gaagctttcg aagatcatac ggatccgctt
    17341 gttgtgtgaa agagcggata aatgttggct ggatcaacag acgtgtcaaa atagcccaat
    17401 cgcttatttc ctgttttttt tcttcttcat ttaagtctgt gagctggata gtcatcaata
    17461 atttatcttc gccaatcatt ttctcacctc tttaaaatct ttttataatt taattataac
    17521 caacgataaa gaaaaactaa aactttatgt cttagaaacc aatttggacg attacatgat
    17581 agactaagta aaaagaaaca aagaggtgtt tacaatgggg ttgaaatttg aacgtacaga
    17641 taatttagat aaacttttcg aatcatttgc tgtcgatccg gaaaaaacag ttgcaccaaa
    17701 agaagagatc gatcgatttc taaaaccaca gccacaaaat gaaaagaaaa aagacaagaa
    17761 acaaaagtaa gttaagctca aaaaaagact taccagccaa acttttatga aagagtttga
    17821 ctggtaagtc ttttttattc tttttatgat tcaattgctt gttcgattgc tgcccacgct
    17881 tcttcttttc cctgttttgt aacggaagaa aagagaataa acgtgtcatt gcgatcaaaa
    17941 tccaacgctt ttttgatttc gctctcatgc ttgttccatt taccacgagg gatcttatct
    18001 gccttagtgg caacaacaat tacagggata tcataatact tcaaaaattg atacatctgg
    18061 atatcttctt gagaaggctt atgacgcaaa tctactaaag aaacgaccgc acgtagctgg
    18121 tctctagtcg ttaaatatgt ctcgatcatc tttccccatt ttgctcgctc agttttggat
    18181 acttttgcat acccatatcc cggaacatcg acaaaatgta atgcgttctc gatcaaataa
    18241 aaattcaatg tctgggtctt tcctggctta ccggaagtac gggctaaatt ctttcgatca
    18301 atcaatgtgt tgataaaaga agatttccct acatttgatc gtcctgccag agctatttct
    18361 ggcaattgtg tatctggata ttgttttggt gccactgcac ttatgataat ttcagcttga
    18421 tggactttca tggtctctct ccttaacaag gtgatgaata aagcaagcgg ctgtcgcaaa
    18481 agccaaaatg acttttacgg cagcctcttt tcatcaaccc gcttttttct ctgaatcttt
    18541 atagattact gttggctcac cagacaattc agctgcttct tttgtgataa tcactttttc
    18601 gatgttttca tttgaaggga tatcaaacat cacatccatc atgatttctt caatgatgga
    18661 acgaagtcct cgagcaccgg tattgcgttc gattgctttt ttcgcaatgg ctctcaacgc
    18721 ttcaggttcg aattcaagtt cggtattatc tagagaaaga agtttttgat attgtttgac
    18781 tagcgcattt ttcggctcag tcaagatccg taccagatca tctgttgtca atttctctaa
    18841 agctgccata acaggtaatc gtccgataaa ctcagggatc aatccgaatt ttagtaaatc
    18901 ttcaggaatg atatgctgca tgacactttc gtcttcagaa agtttttggt tttgtgtacc
    18961 aaatccaatc gtcttttcgc ccatacgatt cttcacaatc gtttcgatgc catcaaatgc
    19021 accacctacg atgaacaata tgttcgtcgt atcaatctga ataaattctt gatgtggatg
    19081 tttacgtcct ccctgaggtg gcacgcttgc aacagttcct tctagaattt tcagcaaggc
    19141 ttgttggaca ccttcgccgg aaacgtcacg agtaatagat acattttcgc ttttacgcgc
    19201 aattttatcg atctcatcaa tatagatgat ccctttttct gcacgttcca cattgtaatc
    19261 agcagattga agcaatttaa gcaaaatgtt ttctacatct tcccccacat aaccagcttc
    19321 tgtcaaactt gtcgcatctg cgattgcaaa aggtacattc aatgtttttg ctaatgtttg
    19381 tgctaaaaat gttttaccag accctgtagg tccgatcaag cagatattgc ttttttgcag
    19441 ttcaacatca tctgtgtttt cttgtgcttg ctgattgaca cgtttatagt gattgtatac
    19501 ggcaacagct aacgagcgtt ttgcgcgatc ttgtccaatc acgtagttat tcaatacttc
    19561 aagaatttct agtggtttag ggacttcagt gaattcgcgt acagcttctt cataaaattc
    19621 ttcatcaatg atttctttac ataggtcgat acattcatta cagatgtaga cccctggacc
    19681 agctacgatc tttttcactt cttcttgggt tttcccgcaa aaagaacagc gtacggtctc
    19741 attgctactt ggattgtcat acatggtctt caccccttaa cttgcaaaaa gtatgcaaga
    19801 aactatcaaa gactttacag tcttttacta tcataacata gtttagtaaa aagaaaaagt
    19861 gtttaacaaa ggatcaactg acctatacct ttgcattttt tactcttaac aaaaaataag
    19921 gaactgtgac aaaatcgtgt cacagtccct tatttcgaat aaacaatttt ctagaagtag
    19981 cttcttcaga aatgatttat tacttgattt ctattctttc gcttctgtcg ctgtacctgt
    20041 gatcaattcg attgcttttt tcattgtcac atcatgttcc agcatatctt ctgtcaacac
    20101 gcgtttgatt tgttcgattg gcatgttgta tgtttctgat aattcttgga tttctgcatt
    20161 gatttcttct tctgttgctt gaagattttc agcagctgca attgcttcga tcacaagatt
    20221 tgttcttgtg cgcatttctg cttcaccttc gaattgttta tgcaagtctt cttctgtaga
    20281 accagttaat tgatagtaca tttcaggaga gataccttga cgttgcatgt tgttcaagaa
    20341 ttcgtccatt gaacgatgta cttcatcatg aaccatcacg tgtggcaatt caacgatttc
    20401 tgcgttttca acagccatac ggatcgctgc ttcatctttt gcatcgtccg ctgctttttc
    20461 ttttgtttct actaattctt tgcgatattt ttctttcaat tcgtctaatg ttgatacttc
    20521 gtcatcaaca tcttttgcga attcgtcatc taattcaggc acttcttttg cttttacttc
    20581 atggattgtc actttgaaga ctgcttcttt tccagcaaga tcttctgctt gataatcttc
    20641 tgggaaagtt acatttacat ctagactttc acctgattta tgaccaacta attgatcttc
    20701 aaaacctgga atgaatgagc ctgaacctaa ttcaagtgag taattttcgc cttttccgcc
    20761 atcaaacgct tcgccatcta caaagccttc gaagtcgatt actactgtat cgccattttc
    20821 agctgcagca tcttctttga taactaattc agcttgtgct tcttgttcac gtttgatacg
    20881 tgcatccacg tcagcatctg ttacttcacg gtcttgtttt tcaacaacta gatctttata
    20941 ttgtcctaat tttacttctg gtttcactgt tacttcagca gtgattaccc agtcttgacc
    21001 tttttccatg ctttcaacat cgatttttgg ttgagaaaca ggatcgatgc cagcttcttt
    21061 gacagcagct tcataagctt ctggtaatac agcattcaat gcatcttcat ataaagcttc
    21121 ttcaccgtac atacggttga atacttgacg agaaactttc cctttacgga atccaggtac
    21181 gttcaagctt tttttcactt tattaaacgc agttgttaat cctttttgga tttcgtcttg
    21241 cgcaatcgta aattttaaaa caccatcatt cgtgcctttt ttttcaaatg ttgcagtcat
    21301 ttaattccct ccgagtttag aaattcattt tatacatcaa aaacgtttca tgcatacctt
    21361 taaatagtac actaaccctt tcaaattgta aacatattga atggatagaa tccttgtttt
    21421 tcataacatt tttatctttt tcactgataa aatctaatgg aagaagatca tttgctgcaa
    21481 aattttttct ctttttttta aaaaatcttg tttttcatct tctgtacctt catacatcgt
    21541 ttttcctgta cacgcctcta cagtcagttg aaaccagcgc ttcgggtctt cgatgtatgt
    21601 atcatagaca ggataaacaa ttgcagattg caacgtgaat tcttcattta acacggaact
    21661 aagcagcgca tcttcattac cgtaacgatc tgaaaataca cataattgct ggtaacttgc
    21721 ctgctgctgc ggctttggca gatcttttgg tatgactttg actaactttt cttcaatcgt
    21781 caaatagaaa acaggatggc attcgttcaa ttgcctaagt gtctccagta attttgccct
    21841 tgctaaaata tggacctctg gcgatacaat aagtttactc gctgtttgga taaactcatg
    21901 ataaggaagc tgttcgatct catcaataga cgcaagctga cgtgcaggtt ccaacgaagg
    21961 aagttcctcc gatttattgt ggagtgcctt gatcttctgc ctctccattt gtccggataa
    22021 ttcttctgca aattggattt tttcaatgac ccgctgctcg aaagcttcat aaaaatctgt
    22081 acttgctatc aattttctag catataaaaa atcttgtgta ctgattaaca agtcaaaata
    22141 aaattcagca aactcctcat tttgaagata ctgtttttca tgcaagagtg cttgcttcaa
    22201 tgcttctttt ttctcgtcca actcgtaaag acaaaaaaca agcaggctat tagcctcaaa
    22261 gctttctttc aactcgtaag ctcgttgaaa taattctttt gcctgctgat attgctggtt
    22321 tgctagtgct tgttgtgcta ctcgcattaa attctcatat ttttctggaa aatcaatcgg
    22381 cttcacgcag tgtcctcctc ttgctcatgt tcagctcatg ttcaatagta gcaaaacaaa
    22441 aaaagcatga caattaaaaa taactgaagc accctctaaa agctagattt tagatctgac
    22501 ttttagaagg tgctttcaac tgttactgct ttttattgct catcagctgt tgacttcctg
    22561 gttgaatgct ttgatcagat tgatcgtaac aacacttgta ttagtgaaaa aatcgcctag
    22621 atgttgcttt ttctgattaa agattgctgg aagataccct aacatcagca aaggaaattt
    22681 tagaatgaac cggcaagcac cttctctaac taaaacggtt gaccagctaa gttcttcttc
    22741 atcaaagctg atgactcgaa taccaaagat cattttccca atcgtttgac catgattgaa
    22801 tttcgtcaat aaaataaaat acgctagata aataagtaag gctgtgaatc cgtagatact
    22861 cagataagaa tctgaaaaat taagccaacc tagatttttg gcaataccaa gtgttaaacg
    22921 cgtaatagct ccgatacaca aaagatcgac caagaaagca aacattcgga taaaaaaacc
    22981 tgcatagaaa tacttaggaa aatcatttgc ttttggcggt tcatttcttg tgtccgtata
    23041 ctttttccat ttacgttgtt tctttttgat ttcttcgggc gtcaaaggct ctctttcaaa
    23101 agagctcagt accttttggg aaaagttgga gtcaggtcct acagcaggct gctgttgctt
    23161 atctttttct tgttccttat tttcatttga aagctctgac atgcttactc acctccgtaa
    23221 taatacattg cttttggtga ttccgctgtc cctagattct caagaacgga taagatctga
    23281 ctagtctcag aaggcttcaa tccctggaat tctgctagtt tacttcctaa ccatgtacta
    23341 gcaaacccag atggcgtgga agaatattct actagttccg catcttcaag ctgttgttcc
    23401 tttcgcatag cttttaaagc atcttcagga aatcccaatt catcgactaa acctacttct
    23461 tttgcttgga caccatcata aatccttcca tcagcaattt ttttgacttc ttcttcagat
    23521 ttattccgtc cctcgctgac gattgctaca aaacgactat atgcgctgtc gatatatgct
    23581 tgaagcaccg cttcttcttc ttttgtctct ggtcgcgttc ctgaacccat atctttcaac
    23641 gctccgcttt taactgttgt atcttccaca cccagttttt ctagaagtcc tgaataattc
    23701 aatcctgaca tgatcacacc aattgagccg gtcactgttt cttctgttgc aaagatttta
    23761 tctgcttgag cagaaatata gtagccaccg ctagctgcca tgtttttcat gctgacatac
    23821 attgggatat catgttcttt acgaatcgta tccaatttct tagcgatttc cgcactttcg
    23881 tagacaccgc caccaggaga atttacttct aaaaagatac ctttgactgt tggatcttct
    23941 tcaatcgcct tcagttgttc cataaaattt ttatgattgt aaccttcaga agcaaataaa
    24001 cctgaagacc ctcccgcagc aatcgttcca tctactgata attttacgat tttttgcgta
    24061 ctgtctccct cttccaacac atttgaagct agttcttcat ccccatataa ccaactgttc
    24121 aatccctcaa tttgatcgtt ttcctgaggc ttagtcaaac tagctgaaac taacgaaacc
    24181 acaaataatc ctgcagcaat cagaactgct gcccaacgtc tcttattcat tctttctcct
    24241 ttctaataca agttttttat gacgatcaca gtttctgact gctcagaagt gtcgaaccgc
    24301 tctgcttttt cttcccgcgc atacccggaa aacgactctt ttgtcaattc atcttctacc
    24361 gtaaactcac gagcttcagc aacttccaag gcatcaggtg cgatttcttg gatgctatcc
    24421 cagccagtat ctaaaaatat tttcgtatca tctgatacac cagctgatgc ttcgaatttg
    24481 gacaattgtt tttttaacat tcccagtgtc atgatctcaa tttttgaatc catttttact
    24541 cctcctctat ccgattactt tctattgtac cgaactcctg aaaaagatga tagataaaaa
    24601 cccattattt tttgacataa aaagagcatt tgacaaagca tttgatatat accaatttat
    24661 tttttattct ttttttgttg acaagaacat tcgcaaaagg ttatgatttt aaatgtttcc
    24721 agataaaaaa caaaagagga ggatagttat accttttagg atagcgccag gtctgtgtaa
    24781 acagatgacg aggagagggt ttatcgaaag attcggcgga tggccctagg gactgcactc
    24841 tacagaaaat ccacaaaaaa tatctgcaaa gataaaacaa aaggattttc ctgcagctgg
    24901 aagcattcat acgaattcat tatagaaagt aggatgtttt ttatgtgcgg aattgttgga
    24961 atgattggat taaaaaatgt aacaccagga ttgattgacg gtctagaaaa attagaatac
    25021 cgtggttatg attcagctgg tatctttgtc tcagatggca caactgatta tctggtcaaa
    25081 gcacaaggca gaattcaaaa tttgaaaaac aaaataaata ttgatactac aggaaatata
    25141 ggtattggtc atacacgctg ggcaactcac gggcaacctt ctgaagaaaa cgcccatcca
    25201 catacttccc aaagtggacg cttcgtgctc gttcacaatg gcgtaatcga aaactttgaa
    25261 gaacttaaaa ttgcttattt agcaaatgac cactttattg gtgagaccga tactgagatt
    25321 atcgctcatc taattgaaac tttcgcgaag gatactacaa cgcaagaggc gtttttaaaa
    25381 gctttgcggg tcatcaaagg ttcttacgct ttcgctttga ttgatcggac agcacctgat
    25441 gttatttacg tcgctaaaaa taaaagtcca ctattaatcg gactcggcga tggattcaat
    25501 gtgattgcca gcgatgcaat ggctatgctt gctcatacga aggaattcgt tgagattgaa
    25561 gatgaagaga tggttaccgt cacatcagaa aaagttatta ttcaaaattt tgcaggtgac
    25621 atcgtagaac gttcttcatt cgaagcacaa gtagattctt cagatatcga gaaaggtacc
    25681 tatccttttt atatgctgaa agaaatcgac gaacagccta ttatcatgcg tcgtatcgct
    25741 caaaaatacg taacagaaga caatcgtgtc tctcttgatc aaaagctggt cgatacattg
    25801 tctgatagcg atcgaatcta tatcgttgct tgcggaacta gttaccatgc ggggttagca
    25861 ggtaaacaga ctctggaaaa attgacacag atccctgttg aggttcattt agccagtgaa
    25921 tttggctata acacacctct tctttcaaaa aaaccattct ttattttctt gagtcaaagt
    25981 ggcgaaacag cagatagccg ccaagttctg gtaaaaatca accgtctcgg ctacccttca
    26041 cttacgatta caaatgttgc tggttctact ctttcgcggg aagcatcctt tacactgcta
    26101 ttgcatgctg gacccgaaat cgcagtagca tcaacgaaag cttatactgc acaaatagct
    26161 gtactaactt tattagccaa agctatcggt gataaaaaag aatttgaaac ttctcttgta
    26221 ttcgatatca ctcatgaatt gagtttagtt gctaatgcga tggaatcggt catcgcccaa
    26281 aaagattatt tagaagagtt ggcagaagta tatctcagcg atacaagaaa tgccttttac
    26341 attggacgtg gcgctgacta caacgtttct cttgaagctg ctttgaaatt gaaagaaatc
    26401 agttatatcc aggcagaagg ttttgccgct ggcgaattga aacatggaac gattgctttg
    26461 atcgaagaag gaacaccagt gattggaatc atcagtgaag aagtgactgg tgcgcatact
    26521 cgaggaaacc tgaaagaagt cgaaagccgt ggcgctaaaa cactagttat tgtctcagag
    26581 ggattagaaa aggaaagtga ccagctggtg ctaccaactg tccaccctta tctaactact
    26641 ctaaccacag tcgtaccgac tcaattactt gcatattacg caacgctatt acggggattc
    26701 gacgttgaca aaccacgtaa tttagcgaaa tcagtgacag tagaataata aaagaacaag
    26761 gaccagtgtc agacatctga cactggtcct tgttctttta ttttttatga aaatatctct
    26821 taatccagtc cgatttcttc tttgactact gccgcgattt tatcgacata gtaattaact
    26881 ttctcatcag taggcgcttc tgccatcact cgaagtaatg gttctgtacc ggaaggacga
    26941 actaggattc ttccttctga tcccatttca gcttctgcct cttcaatcac ttttttgatt
    27001 gccggaacat ccatcgcacc gtttttatca cttactcgga tattcactaa tttttgcgga
    27061 tagattgtta cctctgctgc aagctctgat aatttcttac ctgtctgttt catgatattc
    27121 agtaactgaa ttcctgaaag catcccatct ccagttgtgt taaaatctaa gaaaatcatg
    27181 tgcccagact gctcgccgcc aaagttataa tcgtttttgc gcatttcttc tacaacgtaa
    27241 cgatcaccaa cttgtgtaac gacatctttc aaaccgattc cttccaccgc tttgtggaat
    27301 cctaagttac tcatgacagt cgtaacgatc gtatcttttt tcaatcgatt tttttctgcc
    27361 aagtatttag cacagataaa catgatttta tcaccgtcga caatgtttcc aagttcatcc
    27421 accgcaataa tacgatcgcc atcgccatcg aaagctagtc cagcatctgc tcctttttca
    27481 acgaccatct cagctaaacg ttctggatgg gtagagccta cgccatcatt gatatttaac
    27541 ccgtctggat ttgtgcccat ggtataaaaa tctgtttcaa gatcggcaaa caaacgatta
    27601 acacttgttg aagttgctcc gtttgctgca tcgacacaaa tggtcaaacc ggaaaggtca
    27661 ccgttgatcg tttgtacaag gaattgagaa tatttcaata agccttctgg aaattcttct
    27721 acagttccca atccttcagc tgatggacga ggcaattcat ccacttcagc atctaataat
    27781 gcttcaattt ctagctcttg gtcatctact agtttaaaac catctgcacc gaaaaatttg
    27841 attccattat cttgtgctgg attatgagaa gcagaaatca tgacccctgc gcttgctttt
    27901 tgcaaacgtg tcagataagc aacacctggt gtagagatca cacctagctg gaacacttcg
    27961 atacccaccg ataaaagtcc tgaaatcagc gcttcttcta acatttgacc tgagattctt
    28021 gtatcccgcc ctactaacac gcgtgggcgt tttccttctt cttcatgctg gcttaggaca
    28081 taacctccgc accgtcccag cttaaatgca agttctggcg taagttcctt gttggcttca
    28141 ccgcgtacgc cgtctgttcc aaaatattta cccatagttg taaaattact cctttaactt
    28201 aattttctga ggaatttgtt ctgctcattt gtgttgattc gcttgtttct gatgtttctt
    28261 ctgtacttgt ggtcgagctg cttgtcccag attccgaact ggaagaaatg gttgttgtcg
    28321 atggttccgt tcctgaatca gcagatgaaa tacttctact cgtacttgta ttttcttgtg
    28381 tccgactgct cgtcgaagca gagctactgc ttgacgtttg caacacgggt gtaatctcca
    28441 cactaactga ttcgggcttg atacttaacc cctcttctac tggtatttca attttacgag
    28501 ttgttcgatg attgatacct gagacattga tcggtacagc aatactgtca ccgatcgatg
    28561 caagcaaatc gctagagccg cttacttctg cttccagttt tgaaaaactg aaatcatagc
    28621 tttctacccc ggcagctggt gttccttgct gtatcccata aagtcgtacc gacttgcttg
    28681 gcttgatcga atcagctctc actgttactt gcactggatc agagacgata cttaatactt
    28741 cccctgctgc attcagcgcc tgtattggga cctcactatt aatcccatca tctgtgacca
    28801 tcgaagcgtc cacatcagct actactcgtt tgatttctgc caatgttttg tcacctgtag
    28861 tgatagagac ttttttagga ctaacggaag tatcatcaag ttcatatcca gatggtgtga
    28921 acgttgaagg aatcaacgtt tccacatcga aatcttttat tacctttttc tcaaccgtta
    28981 ctgtaatagt cgatggttcg atctttgctg tgacagcact gctcaaattt tgaattttta
    29041 gcggaacctc atgtgttcct tcgcccagct tagtgatatc tgctgtgacg cggaacatcc
    29101 gtgtatcttc gtttgcctct gcattcaact gaactcggtt tgcactggaa agtttgacag
    29161 tcactgcatt ttcatatcca tggatatagt agcgctttga atcataagaa atcttgattg
    29221 gcaccccagt aactgtttga ttatacgctt catttccaga aagcgtattt ggaacggact
    29281 ggccatttgc attgaagtac agcgcaatac tgaacagcaa tgcaataaaa ctgtaaagaa
    29341 tgcttgtccg tttctctttt gagatcattt gcgatcccct cccttagtca taccgtctag
    29401 gaaagattga atcacgtttt tcttgttttt tgttttctct tcagcaatca gttctctacg
    29461 caaaattgcc aaatattctt gctgggataa gccagccatc agttcattat ctaatgtaat
    29521 gctgacatcc ccagtttctt cagagacaac gatggtaatc gcgtcgctga cttcgcttat
    29581 accgacagct gcccggtgtc gagtcccgaa ttcttttgga atcaacatac tttctgatag
    29641 aggaagatag gcgcttgcta ctgcgatctt tccttctttt acaataactg caccgtcatg
    29701 cagcggtgta ttaggaataa agatattgat cagcagctct ccagtgatat ctgcatctaa
    29761 tgcaattcct gtctcaatat actcatctaa tccagtatgc cgttcgatag taattagcgc
    29821 accgattttt cgcttggaca tatattggat ggctttgtca aaagacaaga tcatcttctc
    29881 atcttcctgc tgttcgcttt ttgattgttt gaaaagcgaa ctgcgtccaa gatgttccag
    29941 tcctctccgg atttccggct gaaaaatcac gatagcagct atcaccccgt acgtaatgac
    30001 ttggttcatc agccaagaca aggtgtgcag accaatgatt tccgctacga ttcggataac
    30061 aataaaaata gctacacctt tcagaagctg aatcgctttt gtgcctcgca gcagttggat
    30121 cagtttgtat acaagatacc agacaaccag aatatcaagt atgttcacca aaaaattcag
    30181 agagaacaag tcaccggaaa acatttgctg ccaatagtcc aaattaaaaa gttccccgat
    30241 ttgaaatgac ataaaaagcc tctcttctct tttgagattt cctctctagt atatcatatg
    30301 ttttaatttt agaggctgat ctgctcgtat ttttcttgag aaaaacgcat aattttcagg
    30361 aaaattgcaa gtatgcgttt tatttttctc tgcaataaat atttttaaac ttcagagaat
    30421 tattcattat attttttctg ttatttttcc tttttttctt ttgtagcttg ttccattttt
    30481 ttacgtcgtt ctgctctggc agctcttctt tcttctgctt caaggtccgt cgttttttct
    30541 tttctagtct taacgggtac atcgatagat tcctctggtt caagtcgttt cttgattttt
    30601 tctaattctc ccgtcagtgc agcatagttg taaaaacgtg ttttagcgtc ttggatagtc
    30661 ttttcaaata aagcttttgc aagatttgga tttgcgttct ctagcgagga aaagcgaact
    30721 tgtttcctca tgaaatcagg catagcagag aaatcaggtt ttttgtagtc caaagtcatt
    30781 gggttcttgt ttttttcgac cattgcaggg ttatatcgat ataatgacca atagccagaa
    30841 gaaacagctt cttttgcttc tttaagcgat tctttcattc caccaagcaa accatgtgtg
    30901 atacaaggcg tataggcaat gattaaagaa ggtccaggat acttttctgc ttcttcaaaa
    30961 gcctttatcg tctgcatttg attggcacct gaagcaattt gcgccacata aacatttcca
    31021 tatgtcatcg ccatcattcc tagatctttc tttgaagcat actttccact agccgaaaat
    31081 ttagcgattg ccgaagctgg tgttgctttt gaggtttgac cgccagtatt ggaatagact
    31141 tcgttatcca aaacaagaat attcacgtct gctccgctgg caagtacatg atcgatcccg
    31201 ccaaaaccaa tgtcgtaagc ccatccgtct ccaccaatca tccattggct gttttttacg
    31261 aatagatcct ttctcttgta tatatcctcc agttcaggga caattgatcg ttcgctttcg
    31321 atcgcagctt gcagttttgc tgctctttgc tgcgttcctt cgctctcaaa catgtgtacc
    31381 aaccagtctt ccatcaattc tcgtagttct tctgatactt ttggcaatac ttctttgatt
    31441 gaacttgcca aagcctcgcg acgagtctgt gccgctagaa gcataccata accaaattca
    31501 gcattatctt ccaataaaga atttgaccat gcaggccctt gaccacattc attggtcgta
    31561 taaggagaag caggagaagc accgccccaa atagaagaac agcctgtcgc attcgcgatc
    31621 atcattcgat caccgaacat ttgtgtcaat aattttacat aaggtgtttc gccacagcct
    31681 gagcaagcgc ccgagaattc aagaagcggt tgattgaatt gtgtagacaa gacagtattc
    31741 tttcctttga ctggatttgc tttttgtctt aatgtcattg caaaagccca attcacagct
    31801 tgttctttct gttcttcata aggcttcatc actaaagctt tttcttttgc gggacaagcc
    31861 tctacacaca gaccacaacc tgtacagtct tccactgaaa cttggatacg gtattttacg
    31921 ccgtctgcgc ctcgcatttc tctcacgata aatccttctg gcgcttcttc catttcctct
    31981 tcatctgcta aaaatggacg gatggcagca tgaggacaaa caaaggcaca ttcattacac
    32041 atcgtacagc gatcactaat ccactcaggt acttctaaag cgacgccccg cttttccata
    32101 gaagccgttc ctaaaggcat ttcgccagtt gtcattccat ttgctacaag tgtaccaaca
    32161 gagagttgat ttccttcctg tgcattcact ggttcaagta tttcttgtat ataaccagtg
    32221 ttccttttat tgattttttc aggcatctcc aacgttctcc aatggtctgg gatcgcgact
    32281 tgatgaagca gttcggtcgt ccgatcgatt gcttgtatgt ttttttctac gatagccatt
    32341 gatttatgtc catagctttt cagggcttct tcttttagca gcggaagcac ctcatcaaaa
    32401 ggaatgatgt cagctaattt aaaaaaagcc gtttccattg ccgtattgat ccggcgtccc
    32461 aaaccgactt cttgggccaa tctcatggca ttgatcgtat agaatctaat gttattttca
    32521 gccaagtaac gttttaattt taagggcaga tgcgtttcta attgttcgtc gctccaaaga
    32581 gtattcaata aaaaaatgcc acctggcttc aatcctttga ccaagtcata gctatgcagg
    32641 taagctggtg tgtgacaggc aacaaggtcc gcatgctcga caagatacgc tgaacgtatc
    32701 ggcgtatctc caaaacgaag atgagagaca gtcagaccgc cagatttttt cgaatcataa
    32761 taaaaatagc cttgcgcata tttatcggtg tgatctccaa taatcttgat agctgattta
    32821 tttgctccaa ctgttccgtc agatccaaat ccccaaaact tggcctggaa tgtttgcggt
    32881 tctgtcaaat ccagactttc ctttggttcc aaagattgat aagtcacatc atccacgatc
    32941 cctatggtaa accgtttttt gcgtatgctt gggtcttttt tcaattcatc aaaaactgct
    33001 gagatttggt cgggagtcac atctttagac cccaatccgt agcgaccacc aataacagcc
    33061 gggcgaagtt cagaatcata taacgcactt tgaacgtcta ataagagagg ttctccacct
    33121 gcccctggtt ctttacttcg atcaagaaca gcaattgttt ttactgtatg aggcaacttt
    33181 tgcacgaatg tttccactgg aaaaggacga taaagatgga tattcaaaaa acctgttttc
    33241 cgtccgtttg cattcaagta atcaatcgtt tgttcaatcg tctgtgcaac tgatcccata
    33301 gaaacaatga cttcttccgc atcgtctgct ccgtaataag tcaccaagtc ataagaagtt
    33361 cctcgtaatc ggttgatctc tttcatatat ttttgaacga tttcaggtaa tttatcataa
    33421 taggggttga tcgtttcccg ttgttgaaaa tggatgtctg gattctggtt cgtaccacta
    33481 acagtcggat gattgggatt catccctcgt ttacgaaatg aagctaattt ctcctgattg
    33541 atcagaggag ctaattcttc atattccaaa acttcgattt tttgaatctc gtgactcgta
    33601 cggaatccat caaagaaatt caaaaaaggt acttctcctt caatagaagc aagatgggca
    33661 acaggtgcta aatccatcac ttcttgtaca ctgctttcag ctaacatagc aaatcctgtt
    33721 tgtcttgcag ccatcacgtc tccgtgatct ccgaaaatat tcaacgcatt tgtcgtcact
    33781 gcccgactag caacatggaa aacacttggc agtagttccc cagcaatctt gtacatattc
    33841 ggtatcatca aaagcagccc ttgagaagcg gtatatgtag ttgtcagtgc accagttttc
    33901 aatgatccat ggaccacacc agctgctcct gcttcggatt gcatttctac tacttttact
    33961 ggttgaccga atacattttc ttttcctgct gctgaccatt gatctactaa ttcagccatg
    34021 gtcgaactgg gtgttattgg atatattgca gcaatctcgg taaatgcata agaaatatat
    34081 gcggctgccg tattgccatc cattgttttc atttttcgca tgattcatca tccttgttca
    34141 tattctctct tattatctgt tccattataa cgtaaaacat tccatttttt cttttattta
    34201 taaaaatatt tcacatatgt ctatttgaat tggtcatcta atttttagac aaacagtcta
    34261 aatcgttgcc tttttttgtc agacatgtat aaactataag cgaaagaggt gttttaacat
    34321 ggatatatca gtgattgatg caacgaaaac aaatacacaa aaaggaatcc tttatggttc
    34381 atcgaatgct cctaaaaaaa tggtcgaatt catcaaccta tcttgtccgt attgccgaca
    34441 atggtttgaa gaatcttatg agctattaga agaagcagtc caaagcgggc aattgcagcg
    34501 agtgatcaaa ttgttcgata aagaaaaaga aagcttgcag cgaggaaacg tgatgcaccg
    34561 gtatctcaca atcagcgatg gtcaaaaagc gatcaaagag atcaaacaaa tattcgatac
    34621 tcaagacgag tggaaacatc tttctctcca agaagtagcg gattttgcag tagataaact
    34681 aaagctgaca gaacaaaaag acgaacagct gagccaagct gtcatcaacg aagctgagca
    34741 agcacatatt cgatttgttc cgactgtcat cttgggtaaa gaaatctttg atgaatctat
    34801 ttcaatagag gaattaaaag aattgatcca aacaaaataa aaaaataagg agcgtaggac
    34861 aaaagtaaag aacacttttg tctcacgctc taaacaccta taaacggcgg aagcagaagc
    34921 aactccttct tatttcttgg agttaaacgc ttctgtccca gcctccttat tccgaataaa
    34981 tggtgtccaa aagcttgcat ctgccagatt atctcatttc ttatgacaag ctgagcaaat
    35041 tatctccacc aaataattta ttcatatttt tgacttgcgc atactaacta attcttaaat
    35101 tttcatacaa aatttctcaa attcccttaa cgagtaatat ttatgtacgg tgattcacac
    35161 tagtatccca atcacttttt aagttatccc gatttcaatt ctacttcaaa aatagttgct
    35221 tttatgtatg ctgttagttg attagatggt cgtttgttga gctaggtcgt ttattttaaa
    35281 caagttgttt ctttagctta tacctatctc gtctaccaaa cgcaagatgg caaacacaaa
    35341 tgcatacgca tctgtatttg ccactttgat tcgtataaag ctaacaactc ctaactcctc
    35401 tcatttaata gttgctctct tttttattca gatacatttt tgctgatcac agcctagtat
    35461 ttcaatggtt ttgaacacta aaccaaattg ttccattaca acattcactg gctgatgaaa
    35521 tggattaata gatttcgatt attcaaagcg attttctatt ggtggattgt gttcccaact
    35581 aacaagacgg attccaaagt cattatataa ctcttcaaga gttaaccctt tcctgttccc
    35641 aatagatgta tctctcccac gctcgatata aaatatggag tttcgaatgc caagacctgg
    35701 caaatctttc cttgttacag ttacactgct actaacttcc ccatataaaa tccagctcct
    35761 ccaaaatgat aatatatttt tcgaccatca tctaactcat gtaacaataa ttgttgtgcc
    35821 ctcttgtcac tcgccaaatg caaacgccac gtgcgatacc atgccatgct acctgcatct
    35881 gcagaaaatc catcaaataa tgcaacaatt gtatcttcta tcacaatcct ttccccatct
    35941 acttcatatt ctaatctaaa atcaaactcc cctcgggtaa tcgcaggtct tggtggctta
    36001 ggacccaata ttattctact taaaaccgca ttcgttccca gatgaaacac agctataaca
    36061 acaataatga ttaatatttt gtttctattt gataaatttc tcattattcg caaatcacct
    36121 ctacattctc acatactagt tcgtaatact cctttaatat caaaatatct actgatcgta
    36181 ataatattcg ctatgaattc ctactactcg tataagaaag gccaaatcat cagcttttac
    36241 ttgctgatga tttggcttgt ttctatttat cgtaattgtt ttctaatcac caaaattgtt
    36301 aacaatcggt tcagatattt cccaattcac tagccttata ttatattgat ttaacaattc
    36361 ttcttcattt attgtatttt gatcaagcgg accaaattgt tctaataatt ctttgtcatg
    36421 taaaatatcc cttctagtaa tgcctctttc cctttcaatg aaagaagcac taggaaaagt
    36481 atgattataa ccattcaacc tatctcccat gtaataatta gcaggtcctg gaaaataaaa
    36541 tatttgctta ttatcactaa cttctaacag caacactaaa tcttctccgc tggctaaacg
    36601 acttgtccat ctacgatatc tccctatgcc ttcgttcatt ccaaatccat caaattcaac
    36661 tattaacgtg tcttctatca catgtaattc ttcattcaat tcatactcaa gtctaaaagg
    36721 aaattctcca tatgtaatct ctggcaatgg tggtgctgga gttctccaaa taaatacaat
    36781 aactgtgaat agaaaaaatc caacgatcga tataaatgtt atgagacctg ctcgaagtaa
    36841 aacccgatat aataaaaatg ctgtatttcg ctttgctata tctagaaaaa taaaaagtac
    36901 tgcaatcata atgatagtaa aagttgtaaa aaaacgcatt tttgccctcc ctttataaac
    36961 atcgattctc aaattctaaa ccaaatcttg gtagttcaag ttcaggtata acgtattcca
    37021 accatacatc tcaattacta tttatgaact tatgattatc actgaataaa tcactccatc
    37081 cccttgttaa aacttctaca ttaattacac ctggttgttt tcgactagct aatccaatga
    37141 acttatcaac catccttatc caccaaccac gagaatcttg accagaagta agaagagcag
    37201 ttctcaagga tgatcgagca gttccttcca gttttttaat taatcaggcc agttatagat
    37261 aaatacttga tcttatctat atcaggactt ctatattaag caaaaattta tgtacaaaac
    37321 taaaatcttc attcagttgt ctccccgctt tttatacgat tgtattaaaa acaatagaag
    37381 cctttccaaa ttatgttact ccagtaaaga tggaattata gtttgacgga agaaaagcaa
    37441 gtataacttt taaaaaaaac tgatacattc gcatctcctg cttcatgtat cagttttttc
    37501 tttggattaa actgcttctt cacgcagttt ttccgcaaat tcatttattt tgcgaattcg
    37561 gtgattaatt cccgattttg atatcgcacc tgagggaatc atttccccta attctttcaa
    37621 gctgacctct ggatgttgaa gccgaagttc tgcgatttcc tgaagttttt caggaagggc
    37681 atgtagaccg acctttgctt cgatcaattc gatgttttcg atttgtttag aagccgcatc
    37741 aatcgtcttg ttcagatttg ctgtctcaca attgactaga cgattgaccg aatttctcat
    37801 atcccgtacg attcgaacat cttcaaatcg gagcattgag ttggttgcac caatcaatgt
    37861 taagaaatca gcaatttttt ctgctccttt taaataagaa atatagccat tgcgccgttc
    37921 aagcgttcga gcatgtagac cgtaataatt taacatatcg caaatgtctt ggttatgctg
    37981 ttcataaata gaaaatattt ccaaatgata acggctcgtt tccggattat tgactgaacc
    38041 tgaggccata aaagcaccac gtaagtaaga acgcattttt tgcgaatttc ccataatctc
    38101 gtccgaaacg tgtccattga acatcatacc atccatgata tcaagatcca tcaacacatc
    38161 ttttgtgcct tgcttcaacc ggacgatata gacattgttc ttttttaatt tcattttttt
    38221 acgaacgagt agctcacttc gtacctgata atgatctttc aaaagagaat agatacgtct
    38281 ggcaattgca gcattttctg tttggacgtt caaaataaac tgctggttca caatacctaa
    38341 agagccgttc attcgcagca aagcagctaa ttccgctttc gcatgttctc gatgaacttc
    38401 caaaccagtt agttctttct tcacatctgc agcaaatgac atcctattct cctcctttct
    38461 ccaacaataa attgcttaat attttgaacc gaaaactaaa cggaacaatt cttctactac
    38521 tttgtctcca tcgtggaaga caccaccatc tttcaattcc aaaaagtcgg tggaaatcac
    38581 tcgacatcct tcatttctca atccgttgaa gtcgtgtgcg acttgtacta gatactcatc
    38641 atagatctct gggtccatat aatcttcagg cactttttcc gtattgacca atactgtatc
    38701 gataaaaggc tgattcaaat gcttgtgtaa aacacgtata tgatcggcat ctgtgaaatg
    38761 ttccgtttcc cctttttggg tcatgatatt acagatatag acggtttcag cagttgtctt
    38821 gagcattgct tcgccgattt ctggtatcac taaatttgga aggatactgg tgaataaact
    38881 tccgggtccc aaaacgacca tgtcggcgtc cataattgct gaaacgactt ttcgagccgc
    38941 tttggcttca cttttatcat ttgtgttgca gacatacaca cggtcaatgg ttttacggtc
    39001 gatcgcgatt ttagattctc ctactgcagt cgttccgtct ttaaagacag catgcaaggt
    39061 caaaggagtt tctgaagaag gataaacatg tccatctaca tgcatcattt ttgctaataa
    39121 ttgaatcgcc tcataagtgc ttccccgcat ctcggaaact gcggcaatga tcagattacc
    39181 gatcgtatga ttggctaaaa actgatcttc ctttttgaaa cggtattgga aaatatcttc
    39241 atagaattgt ggcatatccg ataacgccac taagacattt ctcaagtcac caggaggagc
    39301 catagcgatc gattctctga ttgcaccgct gcttccccca tcatctgcaa cagtcactac
    39361 tgctgtgata tctgctcctt gatttcgcaa acttttcagg ataactggca gaccagtgcc
    39421 accgccgacc actacgatct ttggttttct tatccggtag gttttcattt tcacgaccgg
    39481 ttcaccgtct ctttcctttt atccttgtcc cgatgtgtca cattgacttt atagtttttc
    39541 cctaaagcat ttgccaaacg ttcagtcaat gccacagaac ggtgttgacc gcctgtacag
    39601 ccgattgcaa ctgtcaagct gctttttcct tctttaatgt atccaggcaa tacagtttcc
    39661 agaagatcca aaaatttatg gtagaatgat tgcgtttcat caaaggacat cacataatca
    39721 tagactggct gatcttttcc ggtcaatggt cttagttccg ggatataatg agggttgggc
    39781 aaaaaacgca catccatcac aatatccgaa tcgatcggca acccgtattt aaaaccaaat
    39841 gatacaaatt cgatccgaaa tccttggtct ccctttgatt tgaatgactc attgattttt
    39901 tcacgtaatt gtcgtggtgt caatgtcgtc gtatcaatga tcacggaagc ttgggctttc
    39961 aagtcgtcta gtatcgcccg ttctttacgt atcccttcag tgatcaaacc gtccatcgcc
    40021 attggatgtg ctcgtcttgt ttctttgtag cgagccacta gttcttcgtc tgaagcatct
    40081 aaaaataaga tagaagtgtc gatgaagttc gtattttcga tttcgatcag catgttttgg
    40141 atttcttcaa agaaggaacg agaacgtaaa tccactccta aagccatttt tgtgactttg
    40201 cctgattctt tgattaattc ccagaatttt ggaatcaatc gaggcggcat attatcgata
    40261 caaaaatagc ccatatcttc aaaactttgg atagctactg ttttccctgc accgctcata
    40321 cctgttatga tgactaattg caaattatct gtcataatta cgaactcctc tcacattgtg
    40381 catagtaaaa ttatatcatt ccgggcgtat cattaccagt tttctttttc ttttacaaaa
    40441 agatttggac caaatatctt ttaggatatt tgccccaaat ccaatttttt tacttttccg
    40501 atgctgtatc caatgccttg cttctttcag ctagcgctcg ttcaatcgtc cgatctgcta
    40561 aaatagattg ggcatcgatc actggataat ggttatcttc agcaaattca tgcatcaatg
    40621 acaattctgt gcaacctaag ataacttttt cacagtttag gcgctctacc gcttcttcta
    40681 agatttcgta atatagctct tgattcaaat aatccgattc cttgatctca tgatagatca
    40741 gataattgat tttttcttga agaacagtat caggaatcac tgtttcgaat ccaagatttt
    40801 tgacttcgcg ttcatagatt ccagctttca tacttccttc tgttcctaga atcgctactc
    40861 tccctgtcgt atgctgacgg acaagttcat ttgctgcttc tcttggcata tgaaggatag
    40921 gaatatccgt tgccgcttgt aattcttcaa aaaagtagtg tgcggtgtta caagtcaaca
    40981 caatgaagtt cggctgcaat aaattttgtt tctcaatatc atccagcaga aacggcattg
    41041 gattttcttc tgatcgatct aaaatataag ctgtacgatc aggaactgtt gcatgattga
    41101 ataagacata attcaaatat tcttgatctt ttgtagcttt cgttcggtgg ttgatcaagc
    41161 gaacaaaact ttctgtcgcc atcgtgccca ttccgcctaa aatactgaaa aaattctcca
    41221 tgaactagcc ttctttcaca tgaaaatact ttttgaatct tggaatatag ttatggaaca
    41281 tatatttcat caacaaccag cgcttgattg accggtcttt ttcataaaac acagtagtac
    41341 cgtaacgatt ttctttgatc atttgctcag ctagtttttt gtcttcattt tcccgagcat
    41401 attctaaaaa gatttttttg ggcacaccca tccataatct tgctttatct gattgattcg
    41461 ttccataggt cgtttcaaca aagggtttgt taaacactcg atcttctgtg acgaaacgag
    41521 ccagattcaa gccgttcaat gtcacaaaga aactgctacg accttgtctc aaattgattt
    41581 caaataattt atattctcca tctctaggat catacttcat atcgaaattg gcaaagcctg
    41641 tatattcgat tttttccaaa aatgccttaa tggtttgata gattttttcg ttataatctg
    41701 gcatgatcac aacgtaatta ccaatcgatg ctggtgtagg atcttctaac agtggatgac
    41761 caagacacat catccgaact tgatgatctt catctacata cgcatttagc acacgcatat
    41821 tactatcatc acccgggata aaatcttgta caatcatttc acttttataa ccggcttcgt
    41881 atatccggcc taaaattaaa tcgaactctt ctctagtctc taaaataaaa gctttttttc
    41941 ttccttcaaa ttggacagaa agatactcca cactgtttgc aggtttcaaa gcaactggga
    42001 attcaaaagg caattcctgt tccaaatgtc catttactaa catttcttct ctgacgatca
    42061 atgttttagg atacggaaga tcatattctt cacaaacttc ataaaagctt actttattga
    42121 tcagtctttc aaataaagaa taatcaatat atggacagat aaaatattct gacagctctt
    42181 gtttatgttg ggagatcaat tctgcatatc catcaccaca agcaatcaaa agatattttt
    42241 tactcttatc agaatatctt tcttttgcca aacgaagcat cgtctcgata aacacagggt
    42301 ctttatcaaa tcctggaata acttctacat tgacaatttt actgtagcgg gtaggggcta
    42361 actgatccga agcatatgct tggcagattt tgccgtatgc ttcattaaaa gaacgtgcca
    42421 taccataaac gttcatgtcg cttcctaata atatagggat aaattcttca ttttcaatac
    42481 tgttcatcat tatctctcac ttactcataa tctcttgttt ttcttttcca actacacaag
    42541 gaatatcata ccataaagaa aaaagcaatc aaaggaaact cctccgattg ctcaattctt
    42601 ttttgaacgg attgaactta taggaagttt ttgtctatac caatcagtgt agaaagaact
    42661 accatgtaat gattctagaa cactgatatt aataaaacaa gcaaaaattc atttttttat
    42721 gtttttttaa ctatctcagc tatctatcct ctttatacca ttattttttt caaatagtga
    42781 cctgtataac tttcttttac gttaatgatt tcttctggtg ttccagtcgc aaggatcgtt
    42841 cctccacctt ctccaccttc tggtcctaaa tcaatgatat aatctgctgt tttgattaca
    42901 tctagattgt gttcaatcac taaaactgtg ttaccagcat ctactaatct ttgtaataca
    42961 tgcaacaagc gggcgatgtc atcgctatga agtcctgtcg ttggttcatc tagtatatag
    43021 aaattctttc cattagagat tttgtgcaat tcactggcaa gtttcatccg ttgtgcctca
    43081 ccaccggaca atgtcgttgc tggttgcccc atcgtcacat aacctaagcc aacatcaaca
    43141 atcgtttgca gtttgcgatg aatctttgga atgtgcttga agaattctac agcatcttct
    43201 actgtcattt ccaaaatatc agcaatgctt tttcctttgt aatgcacttc taatgtttca
    43261 gagttatatc gtttgccatg acatacttca caaggaacat agacatcagg caaaaagtgc
    43321 atttcgatct taataattcc atccccgcga caagcttcac aacgaccgcc ttttacgttg
    43381 aaactaaaac gccctttctt ataaccccgc atttttgcct cgttcgtttg agcaaataac
    43441 ccacggatat catcaaatac acttgtataa gtcgctggat tacttctcgg cgtccggccg
    43501 attgggcttt gatcgatatc gatgatcttt tcgatacttt cgtagccgga aattgtcttg
    43561 aatttacctg gcttagcaga attcttattt aatttttgcg ctaacgattt ctttaagata
    43621 ctattgacca atgtactctt tccagaacca gacacacctg tgactgctac aaattctcct
    43681 aatggaaatt caacactaat atttttcaga ttgttttctg aagcaccagt gatcttgatt
    43741 gctttcccat tacctttacg gcgttctttt ggtacaggga ttgatttttt ccctgaaaga
    43801 tatttccctg tcaaagaatt ttcatcatta gctacttctt caggagtgcc tgcagccacg
    43861 atttgtccgc cttgttctcc ggccccaggt ccgacgtcaa tcagataatc tgctgcccac
    43921 atcgtatctt catcatgttc gactacaacg agggtgttgc caagatcacg cattttcttt
    43981 aatgagccaa tcagtcgatc gttgtcgcgt tgatgcaatc cgatcgatgg ttcatctaaa
    44041 atatacaaaa ctccagaaag attcgatcca atttgtgtag ccaatctgat tcgctgcgct
    44101 tctcccccag ataatgttcc ggaagcgcga ctcaaagtta gatagtctag tcccacattt
    44161 tccaagaatg aaagtctgtc atttacttct tttaaaatcg gtttggcaat tactttctct
    44221 tgttcagata acgttaagtt tgagaagaaa tctactgctt tttgaataga caactcattt
    44281 gtttgcccga tattcgttcc attgatttga acagatagag cttggggatt caatcgataa
    44341 cctttacatg tctgacaagt taattctgtc atataaagac gcatttgctc acgcgtgaaa
    44401 tcactgttcg tatcatgata acgacgctcg atattcttca ataccccttc gaaagtcgtt
    44461 tctacatcgc gaacgccacc aaaatcattt tcataatgga aatggaaaag atcatctgtc
    44521 ccattcaaga tgatatcctg atgctcttcc ggtaattctt cgaatggtgt atctaaatcg
    44581 ataccgaaac tttcacaagc ctgcgctaac atttgaggat aatactgaga gctgattgga
    44641 ttccatggaa cgatagcacc ttgactaagt gtcttcgttc tatctggaat cactaaatct
    44701 tgatcgactt ctaatttgac acctaaacca tcacagtctg gacaagctcc aaaaggcgca
    44761 ttgaatgaga acagtcttgg ttctagttcg ccaacagtaa atccgcaata aggacaggca
    44821 tagtgttcgc taaacaacat ttcttcttca ccgatcacgt caaccagagc atagccgtct
    44881 gccaatcgca gagccgcctc taaagaatca aatagacgag aacgaatccc ttctttcaca
    44941 atgatccggt cgatgacgat cgcgatatca tgttttttgt ttttctccag ctcaggcact
    45001 tcacttaaat catagatctc accatcgact cgaacgcgga cgtatccctc tttttggatt
    45061 cgttcgaata tttttttatg ttgtcctttt ttctttacca cgatcggtgc caacacttgg
    45121 atctttgtgc gctcaggcaa agccattact tgatctgcca tttgatctac tgattgactg
    45181 gtaatctcga tatgatcgtt gggacaaatc ggatgtccta cacgtgcaaa caacaagcgt
    45241 aaatagtcat tgatttccgt cacagtacct actgtcgacc gaggattttt acttgttgtt
    45301 ttctgatcaa tagaaatcgc cggactcaat ccgtcgatgc tatctacgtc cggcttatcc
    45361 atttgtccta aaaactgacg agcataagca gaaaggctct ctacgtatct ccgctgccct
    45421 tcagcgtaca gcgtatcaaa tgctagggaa ctttttccag aaccggacaa acctgtaacg
    45481 accacgaatt tatctcgtgg aatcgttaca tctatatttt tcaagttatg ggcacgtgca
    45541 ccgtgaatcg taattttatc gttagccatg ttgtcctcct actttgaagc ttttaattct
    45601 aaaatcatat ctcgcacatt agcggctgtt tcaaaatcca atgctttcgc cgcatttttc
    45661 atttcttttt ctaatttcat caataacgta tctttttctt ctttcgtcat ttcttcatac
    45721 gagacttgta ccgcttcttt tgtatcatct tccgattctt tcgtaataga aatcaaatct
    45781 cggatttctt taatgatcgt ttttggaacg atcccatgtt cttcattgta cttctgctga
    45841 atcgtacggc gtcttgatgt ttcatccatc gctagacgca tagaatctgt tactttgtct
    45901 gcgtacatga ttactttacc ttcttcattc cgagcagctc gtccaatcgt ttggactaag
    45961 gaacgttcac tacgtaaaaa gccttcttta tccgcatcca agatagcaac aagggaaact
    46021 tcaggtacgt ctaatccttc acgaagcaaa ttgattccaa ctaacacatc aaattcacct
    46081 aatcgaagat ctcgaatgat ttctgttcgt tccagcgttt tgatatcact atgcaaatat
    46141 ttgactttga tccctaattc tttgaaataa tctgtgaggt cttctgccat ttttttcgtc
    46201 aatgtcgtta caaacacccg ctggtctttt tcgactcgtt catttatttc gcctactaga
    46261 tcatcgattt gtcccatgat tggacgaact tcgatcactg ggtcaagcag ccctgtcgga
    46321 cggatgattt gttggataac ggtatctgtt tgttcatgtt catatggtcc tggcgtcgca
    46381 gagacataaa tgatctgatt gacatgtttt tcaaactctt ctaaccgaag tggccgattg
    46441 tctaaagcag atggcaaacg gaaaccgtaa tctactagca tctgttttct cgctcggtca
    46501 ccattataca ttcctctgac ttgaggcatg gttacgtgcg attcgtccgc cacgatcaaa
    46561 aaatcttcag gaaagaaatc caatagcgta taaggcggtt caccttcttt acgtccgtcc
    46621 atatgccgag aataattttc aattcccgac gtgtagccca tttctaacat catttcaata
    46681 tcgtaattgg ttcgctgttc taaacgttga gcttccagaa gtttgttttc atttcgcaag
    46741 acagttaaac gctgttctaa ttccgctttg atattcgcta cggcatgctc catatgttca
    46801 tcattcgtca caaagtgagt cgccgggaag attgccacgt gttccgtttc tcctaaaact
    46861 tcacctgtca gtgcattgac ttctcggata cgttcgacct catctccaaa aaactccaca
    46921 cgtaaggcac gttcatctct tgaagccgga aaaatctcca cgacatcacc gcgcactcga
    46981 aaacgaccgc gctgaaaatc tatatcattt ctttcgaatt ggatactgac taagtcacga
    47041 atcaactgat tgcgatctag ttctgctccc tgtcgaatcg aaactacttg tttttggtac
    47101 tcaaaaggcg aacctaatcc aaaaatacat gaaacggatg caatgacgat cacatcattt
    47161 ctttctaata atgaactagt agccgaatgt cgtaatttgt cgatctcatc atttacactt
    47221 gaatcttttt cgatatacgt atcgcttgaa ggcacataag cttcaggctg ataataatca
    47281 taataagaaa caaaatattc aacagcattg ttagggaaaa attccttgaa ttccccgtac
    47341 agctgaccag ccaatgtttt gttatgggca ataattagtg ttggtttatt cactttctca
    47401 ataagattgg aaatcgtata tgttttccct gttccagtcg cacctaataa gatttgtgct
    47461 tttttcccgc cgactacgcc atccactaat tggttaatcg cttcaggctg atcaccagcc
    47521 ggttggtatt tcgagacaag ctcaaagtgt cgggaggtat ctctctcaat cattcttcgt
    47581 cctccttttc attcattcct tttcatttta cgaccaataa aaagaaaagg aaagcaaagg
    47641 cattccttac taaaaaatta taccacaccg aacatacttt cgccactttt ttatcttatt
    47701 atgcatgtta cgttttataa cataaaatgt tagaaactcg catttttttc attttttcga
    47761 gcttacagtc ttgtctgatt cagcactttt cgcctataat aataaaatat tggaaattta
    47821 ggaggttgat tatgaaaaga aagcatttat ttcactcatt tgttttctta atgacaggac
    47881 ttgtgacact tgcgtttgga catgttgcac aagctgacaa cactacaacg gcagagtcaa
    47941 gcacaagcga gacaatgcaa aaaatagagc ccaaaaaaga cgtatacgta attgccagtg
    48001 actctgcttt tgctccattt gaatttcaaa acgcaaaggg cgagtacgaa ggcatcgatg
    48061 taaaattgat ggaaaacatc gccaaactgc aaggatttaa catccgaatg gactaccgtg
    48121 gtttctctgc tgctttgcaa gctttggaat caggacaagc agacggaatg attgccggaa
    48181 tgactgtaac agatgaacga aagaaaagct atgatttttc tgattcttac tttgatagcg
    48241 ggatccaatt agctgtcaaa aaaggagata cttcgatcaa gtcttatgaa gatttgaaag
    48301 gaaaaacagt cggagcaaaa gttggtaccg aaagtgctga atttttaagc gaccatgaat
    48361 ctgaatatgg ctattctatc aaatattttg atgctgctga tcaattgtac gacgggttaa
    48421 aagttggcgc aatcgatgca atgatggatg attatccggt aatcggttat gcgatcaaac
    48481 aaggacaaga attggaaaca ccgatcaaaa aagaatctgg cggaaaatat gcatttgccg
    48541 ttaaaaaagg acaaaatcct gagcttctta atatgttcaa tgaaggattg aaagaattaa
    48601 aaagaacagg cgaatacgat aagattatta aagaatatgt agcggatggt tcagaaactt
    48661 ccagtgagtc ttccactgct gatgaatcaa catttcttgg acttatccaa aacaactatc
    48721 aagcattgtt gaaaggactt tggagaacaa ttctcctaac tttgatctcc tttgctttgg
    48781 ctatagtaat cggtgtgatt tttggattat tcagtgtagc accaatacgc gctcttcgca
    48841 caattgctag tatttatgtc gatatcatcc gaggaattcc aatgatggtt cttgcgttct
    48901 ttattttctt tgggttgcct ggtatcgttg gatttacgat tcctgacttc cttgctggtg
    48961 ttatcacctt gactcttaac gcaagtgctt atatcgcaga gattgttcga ggcgggatca
    49021 acgccgttcc tgtcggacaa atggaagctt ccagaagttt aggtctttct tataatcgga
    49081 caatgcaaaa aatcattttg ccgcaagcga tacgcatcat gatcccatca ttcgtcaatc
    49141 agttcgttat ctcattaaaa gacacaacga tcatttccgc aatcggtgta gtcgaattgc
    49201 tgcaaactgg taagatcatt gttgcacgta caacacaaag tacgtatgtt tacttgatca
    49261 ttgcgatcat gtacttgatt ttgatcacag cattgacaaa attagcaaaa gttttagaaa
    49321 agaaggtgaa gtaagatggc agaaaaaatc ttagttgagc atttagtcaa aaaatacggc
    49381 gataacacag tattaaacga catcaatgtg tccatcaacg aaggagatgt cgtctgtatc
    49441 attggccctt caggttcagg aaaaagtaca tttttgcgtt gcttgaatcg tttagaagaa
    49501 ccttctgacg gtgaaattat tattgacggc gcccacttga tggataaaag cacagatatc
    49561 aacttggtga gacaacatat cggaatggta ttccagcact ttaacctatt ccctcatctt
    49621 tctattttgg aaaacatcat tttagcaccg atggacctga agaaagaatc tagagaagaa
    49681 gcagagaaaa aagcaatcaa attgctgcag acagttggtc tagaagataa aaaagatatg
    49741 tatcctgaaa tgctttccgg tggacaaaag cagcgtgtgg ctattgctcg tgctctagcg
    49801 atgaaccctg acatcatgct ttttgatgag cctacatctg cactcgaccc cgaaatggtc
    49861 ggtgatgtct tgaacgtcat gaaaaagtta gcagaacaag gtatgacgat ggttatcgtt
    49921 acgcacgaaa tgggcttcgc taaagaagtg gccaatcgtg tcatgttcat tgacgacgga
    49981 aactttttag aagacggaac gcctcaacaa atctttgaaa atccgcaaaa tgaacggaca
    50041 aaagatttct tgaataaagt attaaatatt taataaaaac caaaaatgcc cggtcgcttc
    50101 atgacgaccg ggcattttta gtttcttgtt ttttcactta actgtaccag ccgcatcgct
    50161 tgttctcctg cacaaacaag caatcgctct acctgttcca tcgcataaga aagtaccatc
    50221 ggttcattga cgatatcaag tacatagtcg ataccacctt cagaaactat gatgattatc
    50281 ttattctaaa gctggctttt ttgttggtac aaacatgaca actacaaaaa aaccaggcga
    50341 acgtatgatt gttcgtctgg tttttaatta ttaatgactt agcgttttta ctagaaatag
    50401 aaaatttggg caagtctact ttatattttt actagaaaaa ctacttccgc tttttcccgc
    50461 caaaagcatc tttaaactta tcgaagaaac cgtcttcttg ctgttcttca actttgatgc
    50521 cgccagcttc agcgaatgca cgtaatgctt ctctttgttg ttcatttaga tttttcggtg
    50581 tgatcagttt tactttgaca tgttggtcgc ctgttgcacc accacgtaac ttcggagcgc
    50641 cttttccacg taaacggaag ttcgtacctg tctgtgttcc agcagggatc ttcaatttca
    50701 catctccatg gacagttggt acttggactt catcccccaa agctgcttgt acaaaactta
    50761 aaggcaattc atagtaaatt tcagaacctt cacgatcaaa gatatcactt tcttctacac
    50821 gaaaaaccac atacaaatca ccaaatggtc cgccgttttc tccagcttct ccttgaccag
    50881 ctaaacgcat ttgttgtcct tcttctacac cagctggtac attcacttta actgaatgtg
    50941 ctttcttctc atgtcctgag ccatgacagg ttggacaagg ttctttgatt tctttccctg
    51001 tgccatgaca aacatcacaa gtttgacggc tcatcactcg tccaagtggc gtttggcgtt
    51061 ctacattgat cgtacctgca ccatgacatt tatgacaagt agttggttgt gtgcctggtt
    51121 ttgcaccgtt tccaccacaa gtatgacaga tttcttcccg attatacttg atttcttttt
    51181 ctacaccaaa aatcgcttct tcaaaagaca agtcgaccgt atattgcaaa tcagcacctt
    51241 gacgaggcgc atttggatcg actgaccgac cgcctcctcc aaagaaagag tcgaagatat
    51301 cttcaaaacc accgaagccg ccaccagaga agccgccgaa tccaccaaat ccgccaccag
    51361 ctccaccagc accgtaattt ggatctgtgc ctgcatgccc gtattggtcg tatgcagcgc
    51421 gtttttgcgg gtcacttaaa atctcatatg cttctgatac ttctttgaat ttttcttccg
    51481 catcaggttc tttattgatg tcaggatggt attttttcga aagcttacga taagctttct
    51541 ttatttcatc ttctgatgct ccttttgaga gccctaagac ttcataataa tcacgttttg
    51601 ttgccatagg ctttcctcct attggttctc atcattttca cgtcgcttgt tatcatatca
    51661 tattttgttt gaaacaaaaa ccgatacttc aaactatttt tcaaaagaaa aagccaaagt
    51721 agcaactttg gccttctctc gaaattcagt gaactgatta tttatcgtcg ccatcgactt
    51781 cttcaaaatc agcatcaacg acatcatctg ctccgccttg agctgcttct ggattttctt
    51841 gagcttgttg ttgtgcagct tgttcgtata gtttaacagt caagttttga acgatttcgt
    51901 tcaatgcatc gcgtttagtt ttcatttcct cgatgttgtt tgcttcaaca gcggctttta
    51961 attcgtcacg agctgtttct gcttttttca cttcttcttc gtccactttg ccttctaatt
    52021 ctttcaatgt tttgtccaca gagaatagca atgcgtctac atcattacgt aggtcaactt
    52081 cctctttacg agctttatct gcttcagcgt ttgcttctgc atctttcacc atacgttcga
    52141 tttcttcgtc tgttagacct gaagaagatt tgatcgtgat tttttgttct ttttgtgtac
    52201 ctagatcttt cgcagataca tttacaatcc cgtttttatc aatatcgaat gttacttcga
    52261 tttgtggaat accacgagga gctgctggaa tatctgtcaa ttggaatctt cctagagttt
    52321 tgttgtcagc agccattggg cgttcacctt gtaatacatg gatgtctact gctggttgat
    52381 tatctgccgc agttgagaat acttgtgatt tacttgttgg aatcgttgta ttgcggtcaa
    52441 tcaatttagt gaacactcca cccattgttt cgatacctaa tgacaatggt gtaacgtcaa
    52501 gtaaaacaac atctttcaca tcaccagtga tcacgccacc ttggatagca gcacccatag
    52561 caactacttc atcagggtta actgatttgt ttggttcttt tcctgtttct ttacgtactg
    52621 cttctacaac tgcagggata cgagtagatc caccgactaa aataacttcg tcgatttctg
    52681 attgtgacag acctgcatct tttaacgctt gacgtactgg tacttttgta cgttctacta
    52741 agtcagctgt taattcatca aattttgcac gtgtcaatgt catttccaaa tgcaatggtc
    52801 cagattctcc tgctgtgata aatggcaagc tgatttgtgt gcttgatacg ccagataaat
    52861 cttttttcgc tttttcagca gcgtctttca agcgttgtaa agccattttg tcttgagaca
    52921 agtcaatgct gttttctttt ttgaattctg ctaccatgta gtcgatgatt ttgttatcaa
    52981 agtcgtcccc acctagatgg ttgtcgccag ctgttgacaa tacatcaaag acaccgtctc
    53041 ctagttcaag gatagatacg tcaaaagtac cgccaccaag gtcgaatact aagatttttt
    53101 cgtcgcgatc tgttttatct aaaccatagg caagagcagc tgcagttggt tcattgacga
    53161 tacgttctac ttctagacca gcgatcttac cagcatcttt tgttgcttga cgttgtgcat
    53221 cattaaagta agctggcaca gtgataactg ctttttcgac tttttcacct aaatagtctt
    53281 ctgcgaatcc tttgatatat tgaaggatca ttgctgagat ttcttgtggt gtatatgatt
    53341 taccttctac atctactttg taacctgctt caccgatatg gcgtttgatt gatgaaatcg
    53401 tatttgggtt tgtcacagct tgacgttttg caacttcccc tacttggatt tctccatttt
    53461 tgaatgatac aacagaaggt gttgtacggt ttccttctgg gttagcgatg atttttgctt
    53521 cgtttccttc caatactgct actgcagagt tagtcgtacc taagtcaata ccgataattt
    53581 tactcataat taacgtctcc ttctttaatt gaaagtgtaa gttttatatc cgcacaatta
    53641 ttgtgcaaca atgaccatag ttggtcttaa cacgcgatca tgtaatttgt agccttcttg
    53701 tagtacttct acaatcgtat cggctggtgt atcttcagtc gctggaacag tttgtactgc
    53761 ttgatgaaga ttaggatcaa aggcttctcc tttagcaggg attttttcaa ttccttcttc
    53821 ttccagtgcg ttacgcaaac tttccaagac catttccacg ccttttttca aacctgcccc
    53881 ttgatcatct gacacttctg tggctaatgc acgttccagg ttgtcaattg atggaagtaa
    53941 ttttttggct aaatcttgtg aacggtattt ttgtaattgc tcacgctcat tttttcctcg
    54001 atttgccata tttgcgattt cagcacgagc ccgcaagtat ttatcttcca tttcttctaa
    54061 ctcagctttt aatttcgctg tttcgaattc ttccgcttca gcttctgaaa tacccgaatc
    54121 ttctgcagct tcgacatcga tttccggttc tggctgagca tctgtcattt ctttatctag
    54181 ttcttcttga ttttctttca cgatacagtc ttcctttcat tattcagtca gctgtttgat
    54241 tgctcttact aagaaagaga gaacgatcag ctgcaaatag cttcgttatt cacattacga
    54301 gtttgacaag tcaagcgagc ggtagtaatc agcaagttta gctgctagtt ctcttcgata
    54361 aacatcgact agaccgaaca tccgagaata aggcatacta gtaggcccta acaaggctat
    54421 tgtacctctt ccatgaccat ctatctcgta acttgcttga atcatactca tattctgcaa
    54481 taaatcattg cctaattcag caccaatctt gatatgaata ttgctatcca tcggagtgat
    54541 catttgtgta agtccgtctg agtctttcat aaaagaaaac atcgatttga actgatcaag
    54601 atcttgacgg ggctcataat ccaagagatt catttgtcca ctgacataaa ccttttcttc
    54661 aaaaacatga ccaagaacgg tgtcaaataa ctgagacatc ccttctgtcg tctggaaata
    54721 tttatgaaga atcatcggaa tctccgtacg aagtttatgg tacaccgtca tcagaggatc
    54781 tccgactaga cggtcgttta caatctgtac cattttttct agttcctgac ttcccatata
    54841 ttcgggaaga gtgaacactt ggctttctac attccccata tctgttacga cgatagcaat
    54901 aatctgtcga ttattcaagg gtacgatacg aaagccagtc agtcggcggt cttttatttc
    54961 aggtcccaat gaaaaagtcg tatagcttgt cagctcagat aatatctcag cagattgctt
    55021 aataatctca ttgatttcat gaaattctct gcctagagac tgacgaatca gcagcaaatc
    55081 atcttctgct acgcgagcag gttttaacaa atgatccacg taataacgat agcctaaagc
    55141 agatggaatc cgcccagaag aagagtgagt cttcaacagc aatccttcat cttctaatgc
    55201 tttcatatca ttgcgaatcg ttgctgagct ggccttgact ccctcagaca ttaatgtttt
    55261 ggaaccaacg ggcacgccac tactcgtata gtgttggatg atcagacgta aaatgtcgtt
    55321 ttgtcgcttt gttaacatct tcctcacctc tcattagcac tcatatattc taagtgctaa
    55381 ttcacttata aatatagcaa gaagcccttc tcctgtcaag agaaagactc cattttttag
    55441 cactctaaac acaagagtgc taattctaaa taattgatgt aaatccagtt atatcaatac
    55501 ttcaatacct cattgtcaaa ctttctaaac tttttctttt caagtttcga taaaaaacaa
    55561 atattcttca aactattttt caaataaaaa gcgctcaaac acattgttcc ccaaaaaagt
    55621 ccctcttggt gtcaaataaa cacggtcttc ttcattaaca agtaaccctt cttgctctaa
    55681 ttcccttagc acatcaccgt acaccgattc aattgttttt tgatagcgat catagaaatg
    55741 ttttttagta atccctgttt tttttcttag tcctaagaac atttcttctt ctatttgatt
    55801 ttttcgtgtc aatttttctt cttccaatac cgggcgttca tttgctcgta atgggcgtaa
    55861 atagtgttgg attggtcctt tgttgcggta acgaatatct ccaagataac cgctagcacc
    55921 agctccaaac ccaaaatagt ggtcattgtt ccagtacatg aggttatgtt gagattcatg
    55981 tccggagagc ccaaagttac ttatttcata ttggtgacgt ccatgctttt ccattgcttc
    56041 aatcgtctct tcaaacattc tggtttccgt ttcctgatca ggcagttcga gtcttccttg
    56101 tcgtacccag ttcatgaaca ttgttttgtt ttcgaggatc aatgagtaca aagaatagtg
    56161 aggcagatcc aaagccaaag ctttttccaa agtatcccga tatccttcta gtgtttgtcc
    56221 cggtaatgca taaatcaaat cgatgcttac attggtaaaa ttttctgctt ctaaaaattg
    56281 cattgtttga tacacatctt ctgcagtatg tttacgtcca atttttttca ataagcgatt
    56341 gtcaaatgtc tgcactccca tggataaacg attgacgcca tagtttttca tcacttgaag
    56401 tttttcacgg gtcaaatcac caggattcgc ttctacagta aactcattac cattttgaaa
    56461 aggcaggatc tctctagcac ctgataatag tcgatcaagt tgtttagcag ataaagaagt
    56521 tggtgttccg cctcctacat aaagtgtttc caaattcgct gtaggatgac tttccagcat
    56581 taatctcatt tcattcagca gcatttccac atactcatct actggctgcc cttctaaaaa
    56641 aactttatta aagtcacagt aatagcagat atgctcgcaa aatgggatat gaatgtacgc
    56701 agaaaccttg ttttctgcgg gatttattgt ttctatcatt gaaattcttt ctccttacgt
    56761 cgaaaagtcg tgcctgtttt cgaccttctt aaaatcgcgt tcaagcacca actcattgaa
    56821 ataagccgca tatttagaat caaggaaacc tagctctaaa aatgcttgct tattgtcatc
    56881 ggagctacac ggtgctttta cagcctctcg ataaacggta atcgcaaagc tgaccttcgg
    56941 cattaagtca aaatctgcaa aaatatgaaa agctattttc acagattcct ccttaagtgc
    57001 tcagaccaca acgctttgct tacaacctct catatacgca tactaaacgt atttggcgaa
    57061 caaatccact aaaaaatcca agaatcgact tcttggatga tttttttaca ctcatgattc
    57121 ttcctaaaca attttgtcct tattttattc ataaaaaaat ctagtcaaat ctctcacttc
    57181 aagagtttta actagattcc aattattatt tttttgtaaa aataaacagt ctcttaatca
    57241 tagacatttc ttcggtgccc tacttgaata gcgataacaa cgaattgatt atcttctatc
    57301 tctaccaata cacgtatttg ccaatcagag ctgcttctga atttcgggcg tgaattttaa
    57361 acggcaggtc attccagccc accaaattca ttaaggattc ttccatgaat actgttgttt
    57421 tcccacttct tatccaatgt ttatgggcaa tttcagccgt ttgacgattg tattcatctt
    57481 ctagttgttc catcgaatag ttttttagaa gttcagataa agtaatccca taaaattcag
    57541 ccatataaga aagccattct tttttggcgt cacttacacg aaccgtaatc atagccctat
    57601 aattgcctct cttaaagaat ctcatgcatt tcctgatgtg attttagaaa aatttacttg
    57661 ctaaaaaact gtgaaaatgt aggagctagc atccttgtag taaatgatcg agctctttat
    57721 tatacattaa aaaagttcag aaattttgat gaagtagcca actacttttt catgagtaaa
    57781 gcaacactag aaaaagaatc ctccagtact tagaatttac tggaggatgt acttttgaag
    57841 aagcctatag gttatttaaa aaatattact acaggaagga acgagaatta cttcgttact
    57901 tataatagaa cttagtctta tatagaaggc atagctcaca ttagcttatt tgtcctataa
    57961 aaataaatta cggtatttta gaaaagctat tttctctttt ttcaacgatc ttcagcaatt
    58021 tttctttatc gtcaggtaac tcaagttcat gaattctagc atgacaattg ggacataacg
    58081 cgatacagtt ttcaatcaca tcttttccac cttgcgacaa atattctata tgatgacaat
    58141 gtaaataagg tacaccgtct tttataaatg gccctttttc ttggcaaagt tgacaaatac
    58201 cttttgcaag tcgtttaaca tattcagata cgaagggatt acgtttatat atttttgttt
    58261 ttgagaagca aaactcctct tcgttaacat attttgcagc cattttttta ttataattac
    58321 taacttttct tgcttcttcc tctaatttag cattagataa attacgaata tgtctagttt
    58381 gattttcttc ctggttatat atctttatct tcaatcaatt gctcttgatt tattaaagct
    58441 aatggaaatt tatatacttt tctgctgttg cccgaactat ctttttcatt aacagtatat
    58501 atttgtcctg ctaaatatac ttctcccaga tatgtgtgtt cttttggttt aaatgtttca
    58561 aataaatgaa tctttacaaa attacttttt gaattggcta aaactttatt ttgattctta
    58621 tccaatactt gatccccttt tagccccata ccggtatatt gtaaaatacc atttaaccac
    58681 ttatctttat aaacattact tcctgtttga tcactaaaca gtacaagagt attagtttga
    58741 tgagatcttc tcattccacc ctgtttggaa catttaaact tctctgaaat ttctttgtta
    58801 gtatatactc taccaacttc taactcacct aatatgtttt tagaccaatt ttctcgccat
    58861 gtaataatct tatcaagatt aaacatttct ccctcttttg gcatgccatt tctaattaaa
    58921 ttagctaata caccagtaga aatatctaat ttttttagta attctttctt agtaatcaat
    58981 gttggattca taacaatgca gctcctaatg atatcaatat aattgcatta gataacttga
    59041 aaaatcgaaa aaaactcatt ttgtgtcaag ttatctgtaa tatataaaaa tatcattatt
    59101 atacctcatc tatgtgaatt tttactattc ttaccttagc atattcttta taggtatatc
    59161 agagctaatc aaactaaaaa atacccgaag tcttcttatg taaaaacacc ggttattact
    59221 taaatatgac ttaggtaaaa caatgagtga tccaattata aaattttatg gcgaataatt
    59281 tagtaagcgc tacatattga atgatttaga aattttgaaa aagtagctag ttccttttat
    59341 accagaaaag taaacctaat aaaaaacaca actaattttt agctgtgttt tttaagttac
    59401 aataattatc tatttaatag tcttaattca ttaaaataaa aaactcaact ttacaaggat
    59461 atggtttaaa aaatttattt atccaaccca actagttttc gctgcttacg atttgcttca
    59521 tctgctacgt aattcattac tgttttaaat tctttcgctg aggcgttttc tgataaatca
    59581 gcgatacttt tattatactt cttcacgatt tgttgtactt gttctttctc agtcatgtaa
    59641 ttcctcctcc aactcttttg aagtatattg tattggtagt actaattgaa tattatctgc
    59701 tgcataatgc acttcagaaa tgtcgttttg ataatcctta atcattatgt gaaggactac
    59761 ttcaggatgt ctatttttga ctttttcaat attctctctg gacttaaaaa acgcattgat
    59821 aaacatttct tttggcacat gacgtccttc agctagttca cgttttcgag tgaaatccca
    59881 ggcaactttg ggatcttggt aaacatagaa aatcgtaacg cgaaagttat ttttaagggc
    59941 tcgaataata tttttctctg cactaaggat agcaaaagta tcctctagta tgaaagaata
    60001 gccatgttcc agtacatatt ttaatgattg ttcaactaac caagaagaag ctttctgaaa
    60061 aacacttgag ttctttccat tgtattctgg aaattgtgct ctaaaatagt ccgcatcaat
    60121 cacaacatgg ttgtcataat tttaagctaa acctagtgca acctctgttt tacctgctcc
    60181 tgggcttcca gccatgaata ccgcatcttt ttcatcatca gggatttttc ccttgatgat
    60241 tgtttcaata aatttatctt tattgaccct agcgtaatct aaatacttgt ctttaggttc
    60301 caagattgca ccttctttcc ttcttctata ttcatactaa ttatacgata tttatataaa
    60361 aaatataacg gtcattttaa ccatattaac atttagtcac agtaatagca gatatgctcg
    60421 caaaatgaga tatgaatgta cgcagaatcc ttgttttcta cggcattcgc ttttttttca
    60481 ttggactagt tatatatttc tagggttgtt ttcatgtctg agtgcccgat atgatttatt
    60541 tttcgtttct ataattactt tttgccttcc agtttctttt aagattgtac tcaagcaagt
    60601 gggaattggt tcttttgtca tccatgtatg caaaatgtta taatgctggt aaacaaaaat
    60661 gattacctgt catttttatt tagttcgtat ttaattaagt tgactgtctg tatactctgt
    60721 ctaaaactta agaattgttg ttttcgctgt cgctatccaa atttaatgaa tgtctgtatg
    60781 ttaaaactgt tgtccttgtc atagatagta aaggagagct tgaaaatgaa aactgcgatt
    60841 acgtttctat tagaggaata tgccgattgg gaaggtgctt atattactag cacactaaac
    60901 caaaaagagg gatggcaaaa caaaactgct tctatccaat cagaggtcac ttccattgga
    60961 ggattaaaaa caaaaatcga ttacttaatc gaacaaatac ctgaaaaagt tgaccttttt
    61021 attatgatcg gtggcaattc atgggacata aaaaatcatc atttatatca aataatcgat
    61081 aaattgttaa aaacagacgt accagtagct gctatttgtg gggcagttga ttatttagct
    61141 cagagcggat tattgcaagg ttacaaacac accggaaatg cgcaatttcc ttggttagaa
    61201 tcggaaaatt atcagaataa agaagatttt atagaacaac aagctgtgaa agatcgtaat
    61261 ttgattactg cgaatggaac agctcctata gaattcacag acttgattct tcaagcagtc
    61321 cattttgaaa cagaagaaat aagagaaaaa aagattcgtc tatacaaact tggattctat
    61381 gattactata aaaagtacgg agatcctttc tcctaatcat tctcttcaat taaatactcc
    61441 ttatcaatag tatgacaagt aattttactg atggttattt gtcacacttt ttctgacatt
    61501 tatcaaagaa gatacactct atttatattc atagatggaa tggcaacagt ttttttgaca
    61561 aattttataa ggtgcagaac ttctttccgt atgctattcc gattgtcctt gacaatgagc
    61621 ctcctcggat gtgacgatct tcggcaggag ctaacagtta aagttagact gcctcgctag
    61681 cgttagcaca tccgagctca ttatcaagga ttcgctgcgc caatctct
//
LOCUS       DACKAC010000008        61138 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.008, whole genome shotgun sequence.
ACCESSION   DACKAC010000008 DACKAC010000000
VERSION     DACKAC010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61138
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.008"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            386..574
                     /locus_tag="I0B46_000512"
     CDS             386..574
                     /locus_tag="I0B46_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAR2082836.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            652..1665
                     /locus_tag="I0B46_000513"
     CDS             652..1665
                     /locus_tag="I0B46_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAR2082837.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(1732..2766)
                     /locus_tag="I0B46_000514"
     CDS             complement(1732..2766)
                     /locus_tag="I0B46_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAR2082838.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            2881..5106
                     /locus_tag="I0B46_000515"
     CDS             2881..5106
                     /locus_tag="I0B46_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAR2082839.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            5124..7007
                     /locus_tag="I0B46_000516"
     CDS             5124..7007
                     /locus_tag="I0B46_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAR2082840.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            6988..7962
                     /locus_tag="I0B46_000517"
     CDS             6988..7962
                     /locus_tag="I0B46_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAR2082841.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            8621..9643
                     /gene="aroF"
                     /locus_tag="I0B46_000518"
     CDS             8621..9643
                     /gene="aroF"
                     /locus_tag="I0B46_000518"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAR2082842.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            9676..10740
                     /gene="aroB"
                     /locus_tag="I0B46_000519"
     CDS             9676..10740
                     /gene="aroB"
                     /locus_tag="I0B46_000519"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAR2082843.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            10742..11908
                     /gene="aroC"
                     /locus_tag="I0B46_000520"
     CDS             10742..11908
                     /gene="aroC"
                     /locus_tag="I0B46_000520"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAR2082844.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            11925..13016
                     /locus_tag="I0B46_000521"
     CDS             11925..13016
                     /locus_tag="I0B46_000521"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAR2082845.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            13029..14324
                     /gene="aroA"
                     /locus_tag="I0B46_000522"
     CDS             13029..14324
                     /gene="aroA"
                     /locus_tag="I0B46_000522"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAR2082846.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            14317..14826
                     /locus_tag="I0B46_000523"
     CDS             14317..14826
                     /locus_tag="I0B46_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAR2082847.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            14823..15659
                     /gene="pheA"
                     /locus_tag="I0B46_000524"
     CDS             14823..15659
                     /gene="pheA"
                     /locus_tag="I0B46_000524"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAR2082848.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(15722..17458)
                     /locus_tag="I0B46_000525"
     CDS             complement(15722..17458)
                     /locus_tag="I0B46_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAR2082849.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(17455..18384)
                     /locus_tag="I0B46_000526"
     CDS             complement(17455..18384)
                     /locus_tag="I0B46_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAR2082850.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            complement(18384..18962)
                     /locus_tag="I0B46_000527"
     CDS             complement(18384..18962)
                     /locus_tag="I0B46_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAR2082851.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            19235..20521
                     /locus_tag="I0B46_000528"
     CDS             19235..20521
                     /locus_tag="I0B46_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAR2082852.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            20658..21527
                     /locus_tag="I0B46_000529"
     CDS             20658..21527
                     /locus_tag="I0B46_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAR2082853.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            21524..22411
                     /locus_tag="I0B46_000530"
     CDS             21524..22411
                     /locus_tag="I0B46_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAR2082854.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            22578..23261
                     /locus_tag="I0B46_000531"
     CDS             22578..23261
                     /locus_tag="I0B46_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAR2082855.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            23258..24031
                     /locus_tag="I0B46_000532"
     CDS             23258..24031
                     /locus_tag="I0B46_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAR2082856.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            24028..24426
                     /locus_tag="I0B46_000533"
     CDS             24028..24426
                     /locus_tag="I0B46_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAR2082857.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            24611..25282
                     /locus_tag="I0B46_000534"
     CDS             24611..25282
                     /locus_tag="I0B46_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAR2082858.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            25341..25781
                     /locus_tag="I0B46_000535"
     CDS             25341..25781
                     /locus_tag="I0B46_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2082859.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            25793..26461
                     /locus_tag="I0B46_000536"
     CDS             25793..26461
                     /locus_tag="I0B46_000536"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAR2082860.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            26525..27148
                     /locus_tag="I0B46_000537"
     CDS             26525..27148
                     /locus_tag="I0B46_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAR2082861.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            27204..27590
                     /locus_tag="I0B46_000538"
     CDS             27204..27590
                     /locus_tag="I0B46_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAR2082862.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            27803..29194
                     /locus_tag="I0B46_000539"
     CDS             27803..29194
                     /locus_tag="I0B46_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAR2082863.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            29805..30173
                     /locus_tag="I0B46_000540"
     CDS             29805..30173
                     /locus_tag="I0B46_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAR2082864.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            30353..31294
                     /locus_tag="I0B46_000541"
     CDS             30353..31294
                     /locus_tag="I0B46_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAR2082865.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            31363..32094
                     /locus_tag="I0B46_000542"
     CDS             31363..32094
                     /locus_tag="I0B46_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAR2082866.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            32238..34418
                     /locus_tag="I0B46_000543"
     CDS             32238..34418
                     /locus_tag="I0B46_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAR2082867.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            34438..34920
                     /locus_tag="I0B46_000544"
     CDS             34438..34920
                     /locus_tag="I0B46_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAR2082868.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            34936..35697
                     /locus_tag="I0B46_000545"
     CDS             34936..35697
                     /locus_tag="I0B46_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAR2082869.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            35828..37162
                     /locus_tag="I0B46_000546"
     CDS             35828..37162
                     /locus_tag="I0B46_000546"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAR2082870.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            37469..37954
                     /locus_tag="I0B46_000547"
     CDS             37469..37954
                     /locus_tag="I0B46_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAR2082871.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            37947..38630
                     /locus_tag="I0B46_000548"
     CDS             37947..38630
                     /locus_tag="I0B46_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAR2082872.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            38756..41401
                     /gene="polA"
                     /locus_tag="I0B46_000549"
     CDS             38756..41401
                     /gene="polA"
                     /locus_tag="I0B46_000549"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAR2082873.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            41446..42282
                     /gene="mutM"
                     /locus_tag="I0B46_000550"
     CDS             41446..42282
                     /gene="mutM"
                     /locus_tag="I0B46_000550"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAR2082874.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            42246..42875
                     /locus_tag="I0B46_000551"
     CDS             42246..42875
                     /locus_tag="I0B46_000551"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAR2082875.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            42927..43259
                     /locus_tag="I0B46_000552"
     CDS             42927..43259
                     /locus_tag="I0B46_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAR2082876.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            43571..44062
                     /gene="nrdR"
                     /locus_tag="I0B46_000553"
     CDS             43571..44062
                     /gene="nrdR"
                     /locus_tag="I0B46_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAR2082877.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            44085..45458
                     /locus_tag="I0B46_000554"
     CDS             44085..45458
                     /locus_tag="I0B46_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAR2082878.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            45458..46384
                     /gene="dnaI"
                     /locus_tag="I0B46_000555"
     CDS             45458..46384
                     /gene="dnaI"
                     /locus_tag="I0B46_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAR2082879.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            46513..46953
                     /locus_tag="I0B46_000556"
     CDS             46513..46953
                     /locus_tag="I0B46_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAR2082880.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            47097..47798
                     /locus_tag="I0B46_000557"
     CDS             47097..47798
                     /locus_tag="I0B46_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAR2082881.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            47795..48916
                     /locus_tag="I0B46_000558"
     CDS             47795..48916
                     /locus_tag="I0B46_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAR2082882.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            48931..50163
                     /gene="pepT"
                     /locus_tag="I0B46_000559"
     CDS             48931..50163
                     /gene="pepT"
                     /locus_tag="I0B46_000559"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAR2082883.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            50295..51203
                     /locus_tag="I0B46_000560"
     CDS             50295..51203
                     /locus_tag="I0B46_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAR2082884.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            51238..51510
                     /locus_tag="I0B46_000561"
     CDS             51238..51510
                     /locus_tag="I0B46_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAR2082885.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            51521..52567
                     /locus_tag="I0B46_000562"
     CDS             51521..52567
                     /locus_tag="I0B46_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAR2082886.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            52818..55427
                     /gene="clpB"
                     /locus_tag="I0B46_000563"
     CDS             52818..55427
                     /gene="clpB"
                     /locus_tag="I0B46_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAR2082887.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(55578..56249)
                     /locus_tag="I0B46_000564"
     CDS             complement(55578..56249)
                     /locus_tag="I0B46_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2082888.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(56242..56736)
                     /locus_tag="I0B46_000565"
     CDS             complement(56242..56736)
                     /locus_tag="I0B46_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAR2082889.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(56756..57976)
                     /locus_tag="I0B46_000566"
     CDS             complement(56756..57976)
                     /locus_tag="I0B46_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAR2082890.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            58123..59958
                     /gene="lepA"
                     /locus_tag="I0B46_000567"
     CDS             58123..59958
                     /gene="lepA"
                     /locus_tag="I0B46_000567"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAR2082891.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(60052..61134)
                     /locus_tag="I0B46_000568"
     CDS             complement(60052..61134)
                     /locus_tag="I0B46_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAR2082892.1"
                     /translation="MNDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTDT
                     RVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINAE
                     FKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKIIS
                     LEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVKN
                     DGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKEDG
                     ELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISIT
                     MDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT        19946 a        10862 c        13157 g        17173 t
ORIGIN      
        1 tttagttagt ctacattatt ttggcgattt gccaataagg actgttgagt tgtgcaaaat
       61 caaatctggg tcagaatgaa cgtcattctg tctcagattt ttcgttattt caatttgatt
      121 aactgattga tcaaaaagat ttttattttc atattctcaa atgattcaac actaaatata
      181 catgtttgac gcggaaatta ttattcgatt ttggcggcat caggggggat gacgacgtag
      241 cagacccatt attttactca agaaaatgtc cgtgcctatt atttagaata cgatagtgaa
      301 cgtgctggga gttttgttct tttagagaaa gtctctgaag ataaattagt tgtcttagat
      361 tttgactgta acgaaaaatg gcgaaatgga agagcaccag caagtgattg accggatcta
      421 cgaagcgaca cgatatctgc ctttggatcg tttgtgctta agtcctcaat gtggatttgc
      481 ttcaacagaa gaagaaaatg ttttgtttga agaacaatgg aaaaaatttc ttttgtcaaa
      541 agcattgcag aagaagtttg gggaaaatca ttaaatcatc taggagagct tgataggaaa
      601 taacactatg gtataatcag aatgcttaat actaactact gagggagcct tatgaaaaag
      661 aaaattctta aatttatcgg atggttcttc ggaatcattc ttgggatcgc gtttgcaatc
      721 tttcttgcct tccagctttc ccccagacct ggagcattct tgatcaacca tatgttttca
      781 aatactgttc aaatcactga taaaaaaacg ttcgatcaag cagaaaaaaa cgtggcgaaa
      841 aaaacagatt tgacgtatga gtccaagtat aaagagaata cttatgacat ctactatcca
      901 aaaaacagca aggggcctgt tcctgttcta ttttgggtac atggtggagg attcgtcggc
      961 ggagataaat cgggtgtcaa agaatttgcg acaaaattag tttcagacgc caacatcgca
     1021 gtggttgcga tgaattatga attagcgcct gattcagaat accctaatca agttcttcaa
     1081 gtgaatgaac tgatcaaaga tttattagct gaagaaaaag acgacaaatt gctagacatg
     1141 gaacaattat tttttggtgg agatagtgca ggttcacaaa tcgcattgca atatgcagct
     1201 atacaaacga atgatgcgta tgctaaacaa atgaaaatca aacagctact tccaaaagat
     1261 agtctgaaag gaaccatttc ttattgcgga cctgttgatt tgaagctaac agcacagcag
     1321 cattcggatg atcgatttat gaagtttttc gtcaaaaccg ttgcgtggtc gttgattgga
     1381 acaaaagatt ggaaaacgag tgagcaattg aaagaagcta gtgtggctga ccatgtttct
     1441 aaggaatttc cgccaacgta tataactgac ggaaatgcat actcattcca ggagcaaggt
     1501 caagcattag aaaatcggtt gacagaatta aaggtacccg tgcaatcctt gttttataca
     1561 gatacaaaaa aagagattac ccatgaatac caatttgatt acacgctaaa agaatcgcaa
     1621 aattgttacc agcagacatt ggattttgtg aataagtata agtaagaggt tgtgaaaaag
     1681 ccgtttcgtt ttgagtatga agaaacgaag cggcttttca caacctctct ttcaatacct
     1741 ttttgttctt gggataaaac tttccctaac aatcaatcgt gcaggataag tcaaaatgat
     1801 tggttcgtcc cgtataccgt caattcgctc caatgccatt cgaacagctg cttgcccaat
     1861 tgatttcgca ttgatttgaa cactggtcaa gccaggggag aggtttgcga ttgtctcgat
     1921 attatcaaag cttgctacaa caatctcttc tccaatttgg atattttttt gtttcaatgc
     1981 ccgatagaca ccaattgcca ttggatcgct cccgacaaga atggcagtag gaagagggga
     2041 ttcattttct aatagattag tcgcaagtac ctcaccttgt ttttctgtcc agccacctag
     2101 atgataagac atatattttt ctaagtgatg atcgatcata aaatgctgat aggcacgcat
     2161 cctcttttca tttatagaaa tctgcttccc accgaattca tccatatcga ctttatatcc
     2221 cccgatatag gctattcttt tatgaccatt ttctaagaac aattccagta cggttttagt
     2281 catccgttca agatctgcat acaccatatc gacctcttgc tggatgtcag ggttatccac
     2341 gacgactatt tgcatgtttt gttttaacaa ggcggtaatc gattctttcg taacagtccc
     2401 aacaacgatc accgcaccta actgatctag atcttcccat tgtttaggat tatcagatag
     2461 attatagatt cgattcatcc ctaaatgcag acgtcgagct tcttcttcca tccctactcg
     2521 caaatgtttg taatagatat cctgttcttc gctcgctttg ccgatcgtag agatcacacc
     2581 aatcgaaggc attctctttt tgatatattt acgagtactg atgtcataat ccaacgcagc
     2641 agctgcctct actatccgtt gccgagtagt ctgagccaca tggatcgtct gatcgttatt
     2701 caacacacgg gaaactgtag caggagagac atttgccttt cgtgcgacat ctctaattcc
     2761 aaccattttt acacactcct caaaaaaata aaaaattttt attgacaaaa ttgtttagta
     2821 aacagtatac aggaattata aaaaaaagta aacggtttcc gttttgttgg gagtgaaatg
     2881 atggatatta cgcatagaaa aacagataaa aatttcatct tatttgatga aaagagccga
     2941 gtcttccatt tacgaaatac atttctttct tatctgataa agatagaaga aagcaatgta
     3001 ttggcgcata tttattttgg caaaccagtc aagcagtaca aagacaataa aaattatccc
     3061 cgaagagatc gtggattttc aggaaatgtt cccttaaatc ctgaccggtc tctgtcgaaa
     3121 gatacactac cgcaagaata ctcttctcat ggcagcatgg actttcgaac gcccgcatcg
     3181 atcattcagc gcaagaatgg atcagactta ctggatctga gatatgacag ccattatatt
     3241 acagatggga agccagagat cgaaggcttg cctcaaactt atgtgatgga caagtcagag
     3301 gcacaaacgt tggtgatttc acttaaggat agagaaacag cgatttattt cgatttgttc
     3361 tataccatat ttactgatag agcagtgatc acacgatctg taaagataag aaatgaaacc
     3421 ggagaaacaa tcaaactaga aaaagcggct agtttccagc tagactttgc ccatactagg
     3481 cgatttgatg aagtcattgc tttgccagga gcacatgtca atgaaagaca gatttcacgg
     3541 caatctgttt tatctggaac aaaagtattt gaaagccgtc gaggaacgag cagtcaccat
     3601 atgaataact ttattgcttt agttcatcat catacgacag aaaatactgg agaagcgatc
     3661 ggacttcaat ttgtttattc cggcaaccat tctttcgaac ttgaaaaaga tcagatcaat
     3721 caattgagag tcgtcggtgg gatcaacagc catcgttttt cgtgggaatt gaatgcaggc
     3781 cagtcttttc aaacacctga aatgatcctt tcttactcaa gtcagggatt gaacaaaatg
     3841 agtcagatcc accatgagtt gctacgtgaa agaatcgcaa gaggtcgtca tcaatttgct
     3901 gaacggccga ttctggtcaa taactgggaa gccacttact ttgacttcaa tagtgaaaaa
     3961 atcaaggcga tcattgatga agcaaaggag ctaggcatcg aaatgttcgt attagatgat
     4021 ggctggtttg gaaaaagaga tgcggacaat tcttcattag gagactggtt cgagtatgaa
     4081 ggcaagttga caaatggatt acgtgaaata gcagattatg ctcatagcaa aggattgaaa
     4141 tttggtttat ggtttgaacc tgaaatgatc tcggttgatt ctgaactgta tcgaacacat
     4201 cctgattttc ttatgcagga gccaggtaga atgccttctg cttccagatc acaacatgta
     4261 ctggatttta caaggttaga tgttcggcag acgattgaga aacaaatgag aaagatttta
     4321 gatacgattc ctcttgacta catcaaatgg gatatgaacc gttcgctttc agatgtctat
     4381 tcgattaccc ttgacccaca gagacaagga gaagtagcgc atcgttatat gctcgggctt
     4441 tatgaactac ttgaacattt gacaactgat tatccggaaa tcttatggga aggctgttct
     4501 ggtggaggtg gacggtttga tgccgggttt atttattata tgccgcagtc atggacaagt
     4561 gataacacgg atgcagtgga gcgtatgaaa atccaatatg gaacaagcct tgcttatccg
     4621 atttcgtcca tcacagctca tgtatcagct gtaccaaatc atcagaccga cagatctact
     4681 tcgttgaaaa caagaggaga aacagctatg agcgctgttt ttggttatga actggatttg
     4741 acaaagctgt ctcctgaaga aaaaaagcaa gtaaaagacc aaattatatc ctatcaaacg
     4801 attcgtccgg tgatccagta tggccattat tatcgtttgg cgagtccttt tgaagaaaat
     4861 attgccgcat ggatgtttgt gtctcctaaa caagatgaag ctattgtgtt tttagggagg
     4921 atactagctt cagctcaacc agcgttccat gaagtatatc tgatggggtt agatgatgag
     4981 gcactttatc aggaacagac ctcgaagcgg atattttcgg gggccgaatt gatgacagtt
     5041 ggactttact tccccgattt tcaaggtgat ttccaaacag aactgcttca tttcaaaaag
     5101 ttatgagaga gaaggaaaaa agtatgaaag caaatataaa atggttagat gatccacaag
     5161 tttttagagt gaaccaatca aatgcacata gcgatcatgt ttgttatgca acaagggaag
     5221 aagtagaaac cacatcctcc cttaaacagt ctttaaatgg atcatggcgt ttttgttatt
     5281 cgccaaatcc ttcggtacga ccaaaaacat tttataaaaa agattttgat gcctgttctt
     5341 ttgatgaaat cactgttcct agtcatattg aattagcaaa ttatgacaag atccattaca
     5401 tcaatacgat gtatccgtgg gagggacatg tatttcgccg tccccctttc acgacaagcc
     5461 ctgagaaaga ggcggaaggg atcttttcag ctgctaaaga caatccggtt ggttcgtatt
     5521 tgacgacttt tcgattagaa gaaggtctaa agggaaaaca aatctcgatt cattttgctg
     5581 gtgtagaaca agcaatgttt atttggttga atggtcattt tatcgggtat gccgaagatt
     5641 cgttcactcc gtctgaattt gatttaacac cttatttgga agaagaaaat attctagctg
     5701 tggaggttta caaaagaagt acagctgcat tcttggaaga tcaggacttt ttccgatttt
     5761 ttgggatctt cagagatgtc ttcttagttg ctcgaggaca aaaccattta gaagatcttt
     5821 ggatcaaacc tcgcttagat gacaaatgtc aaaatggcaa acttgaagta gatttgtctt
     5881 ttgagcaagt agccaaaaac agtcagctga atgtagcgat actagatcct gatgggaagg
     5941 aaatctatca acagcaatat ccgttaaaag agcaaatgac gattcgcatc gatggatttg
     6001 aaaatgtggc actgtggggg cattttcagc ccaatttata taagcttgaa ttggagacga
     6061 tagacgaaga aaaaagagta acggagtata ttgactattc cttcggtttt cgtaaaatcg
     6121 aaatcaaaga taaaatcatc tacttcaatg gaaaacggat gattatcaac ggggtaaatc
     6181 gacatgagtg gcatccgaaa aaaggacgcg ccatcactat agaagatatg gaaaaagatt
     6241 tggctatcat aaaaaatgca aagatcaacg gcgtacggac atctcattac cccaaccaga
     6301 tcacttggta ttatttatgc gaccaagcag gaatccatgt gatggcggaa acgaacctcg
     6361 aatcccatgg ctcatggcaa aaaatgggcg catttgagcc ttcttacaac gtaccaggtg
     6421 cagtacctca gtggcaggcg gctgttcttg atcgagcaaa aacgaatttc gaaacattaa
     6481 aaaaccatcc aagtattctt ttttggtctc ttggaaatga atcttatgcg gaagaaaaca
     6541 ttcgacaaat gaacgattac ttcaaaaaag tagaccccga tcgacttgtc cattatgaag
     6601 ggaattttcc aaatccagca ttcgaagatt cgatttcaga tgtaaaaagc tgcatgtatg
     6661 cgccaccaga agagatcatt gactaccttg aaaataaagc ggaaaaaccg ttcatcttat
     6721 gtgaatatat gcatgacatg gggaattcgt taggtggaat ggattcttac atgacgttgc
     6781 ttgatcgtta tgaaatgtac caaggaggct atatatggga ttttaaagat caggcgatcc
     6841 aagtgactga tccaatcaca ggcagagaag tgatgcgcta tggcggtgac tttgacgata
     6901 aaccaagtga ctatgaattc tcagggaatg ggatcgtttt tgcagatgga caagaaaaac
     6961 ccgccatgca ggaggtaaga tattattatg aaaaatacag taaataaaag tcatatggat
     7021 acggaaaaag ttgcaatcgt cttcggcgac tgtacattag gtgtcaaatc ggggaatacg
     7081 cattatattt tttcttatac aagaggcgga ctggaatcgc tcaataaaaa cggaaaagaa
     7141 tggctatacc gggaaacact tccagttttt tggcgagcaa cgaccgataa tgacagaggt
     7201 agcggatttg gatttaaatc cggtatgtgg ttaggagccg gattgtatcc aaaagtcaaa
     7261 gaaatagaag tactggtgga caataaaaag atcgatttgc cgattgctcc agtaaataat
     7321 caatattcaa gtaatgaata cgctgaaacg gttgaaatca tttttacact ggaaatcaac
     7381 acagtaccaa aaacccaagt gacaatttcc taccaaatcg ctggagaggg taaaatgctt
     7441 gtcaatatgc attattatgg acaaaaagga ttaccggaat tacccgcatt gggtttaagg
     7501 ttcattatgc caacgaaagc aaaatttttt gagtatgaag gcctatcagg ggaaacatat
     7561 ccggatcgga tggctggtgg tgtcgaaggt atttataaag tggcaggact ccccgtttcc
     7621 ccataccttg ttccccaaga atgcggcatg catatggaaa cgaaatgggt agaactaaca
     7681 agaaatcaaa cgttggataa tacggataaa gatcaagaag atttttcgtt gcgatttgaa
     7741 cagcaggata cgccatttgc tttttccgct ttaccattca aagcagaaga actggaacag
     7801 gcaacacata tagaagaact accgccagaa agacgaacgg ttcttacgat ttttgcaaaa
     7861 gtaagaggag tcgggggtat caatagctgg ggagcggatg ttgagtcacc ttaccgaatc
     7921 agcagcgaag aggaccatga aatcggcttt attattcagt agcaatcttt agattattat
     7981 ttgaaaaacg gagtttacaa gacaaagctg ctttattcta tatgtcatga acaagagagt
     8041 aaaaaagttc tcagatactt ttgttcatga ctattttctt caataaaacc aattgttatt
     8101 tttctgaaaa acaaagttaa ctatcagaaa attaaaaaaa gtttcataaa aatgattgac
     8161 agtcgatttt tttatgtcta tactaggtac aattcttaca tcagtttgga gcgatttttt
     8221 atgaaatcta gaaaacttac aaaactgaat acgtattact ttagctattt tagataggtt
     8281 tgtattcatg acttcgtggg tacaaacaaa ccaggtttgt atctatgatg gctaaagatt
     8341 tttaaacacg acttacagcc acgtagagaa attcatctat agtggctatt ttatattttt
     8401 gcagcgcttt ttaaggctcg ttgaagatgt tcactgtaga ggcaatttcc tacagggaca
     8461 tctttttttg ttgattatct gggtcataag taggaaagaa ggagcgaaaa tgagaacaac
     8521 aggacacggt catttagtag gaattaatgt aaaaccacct tgaaaaagta gagaaaatca
     8581 gccaagatag ataagtaaac aaaagaaagc gggaaaaaca atgatagtaa ttatgaaaaa
     8641 agaagcaacg gaagaacaaa taaaaaaagt cgtcgatcat atcgaaaaag aaggattcaa
     8701 agtctcaatc gatcaaggaa cagaacgagt agtgattgga ctaaaaggtg atacgcgaag
     8761 tttacaagaa ggagcattta cacgctatga aggagtagaa aatgctgtgc gtattttaaa
     8821 cacatacaaa ttgacttctc gagaattcca tcctggcaat acagtcgtgg atgtagacgg
     8881 agtaaaaatt ggtgacggca gtttcgtgac gatggctggt ccttgttcta tcgaaggatt
     8941 agaccaaatc agagaatgtg cgcgaatggc caaagccggg ggcgcaaaaa ttctgcgagg
     9001 tggtgccttc aaaccacgta cttctccata tgctttccaa ggtttagaag aagaaggatt
     9061 gaaatacatc cgtcaagcgg ctgatgaatt tggaatgaaa gtgattactg aagtgatgga
     9121 tgaagggcat atcgatatgg tggctgagta cagcgatatc ctacagattg gcgcacgtaa
     9181 tatgcaaaat tttaaactgc tatctgctgt cggtaaaaca ggtaaacctg ttggattgaa
     9241 acgtggaatt tccggtacga tcaatgaatg gcttaatgct gcagaataca ttgcagtcga
     9301 agacaaatct cctgtcattt ttatcgaacg tgggattcgg acttacgaaa cagcgacacg
     9361 taatacgttt gatttaagtg ctgtcccatt gatgaaaaaa ttaactcatt tccctgtgat
     9421 tgttgatcca agtcatggta caggaatttg ggaacttgta ccaccaatgg ctcgtgcggg
     9481 tgtggcaagc ggagcagatg gcatgatcgt agagatccat cctgatcctg caaatgcttg
     9541 gtcagacggt ccccaatcgt tgaatgaaaa gacttactca cgcatgatga aagaagttgc
     9601 gatcatgaaa gaagcaatgg aaaagattca gacactcgat taattccagc tttttaaaag
     9661 aaagaagtga aacgaatgct tactgtacat gttgataacc aaaaatacga tattcatatt
     9721 gaaaaaggcg cactagaaca ggctggatca tggcttcaaa cgatttggag acctcaaaaa
     9781 gtagctgtga tcactgatac aaacgttgct ccactatatg cagaaaaaat catgttccaa
     9841 ttgagacaag ctaattttga accagtcctt tcaatcgtac cggcgggtga gaccagtaaa
     9901 tctttagaac aagcagctga gttatacgat tttttagcag ataatgattt tacccgcagc
     9961 gatgggatac ttgcacttgg tggaggaatg gtgggtgatc tagcagcatt cgtggcctcg
    10021 acgttcatgc gtgggatcca tttcgttcaa attccgacta cgctacttgc acaagtagat
    10081 tctagtattg gcgggaagac agcagtcaat acaaacaaag cgaaaaacct tgtcggaaca
    10141 tttagccagc cagctggtgt attgattgat cctgaggttc taaaaacttt gccggtacgt
    10201 cgtttacgag aaggaatcgc tgaaatcatc aaatcagcag ctatagcaga tgcaacgctt
    10261 tggaaacagc tgaacgagct gaaagacgaa aaagatcttc tggaacatgc tgttccaatc
    10321 attactgcta cgttggaaat caaacgaaaa gtagtggaag aagatgtctt tgaccaagga
    10381 aatcgactta ttttaaattt tggtcataca attggccatg caattgaaaa tacagcagga
    10441 tatggggtag tcagtcatgg agaaggagta gctattggaa tggtagccat cacttctcat
    10501 gcagaaaaaa tcggcttgtc tccaactgga tcgacagctg ctttagaaag gatacttcaa
    10561 aaattccatc tgccgattac gtacgatcta agtcatccag atgaattatt ccacgcgata
    10621 acccatgata agaaagcaag aggaagccaa ttgaatatca tcttgctaga aaagattggg
    10681 caagcaaaaa tcgttcctgt tcccacagag acattcaaag attacttagg aggactgtaa
    10741 gatgcgttat atgactgcgg gagaatccca tggaccagaa ttaacagcca ttattgaagg
    10801 actaccagca ggattacctc tgtcagaaga agaaatcaat cacgaactcg ctcgcagaca
    10861 agctggttac ggcagaggag gaaggatgaa gatcgaaaaa gatcgtgttc ggatcacgtc
    10921 tggcatccgt cacggcaaaa caattggttc acctgtcact ttagtggtag aaaataaaga
    10981 ttggaaaaat tggacaactg tcatgtctgt agcagaagtg cctgaaaaag accaatcact
    11041 gcggcgagtt gcacgtccgc gtccaggaca cgcggatttg gtaggcggta tgaaatacca
    11101 tcatcgagat cttcggaatg tgcttgaacg gtcctctgca agagagacga ccatgcgtgt
    11161 agcaatcggc gcaattgcca agaaaattct ggaagaactt gacatcatcg tagcaggaca
    11221 cgtgatttgt cttggtggaa tccaagcaga aatacctgga aatatctcga ttcaagaaat
    11281 caaagaaatt tctgaagcgt cagaagtacg agttgttgat ccagcagttg agcaaagaat
    11341 aaaagatttg atcgatcaga caaagaaaaa tggagatacg atcggaggaa ttgtagaagt
    11401 gctcgttgga ggcgttccag ctggattagg aagctacgta caatgggaca cgaagctaga
    11461 tgccaaaatc gcacaagctg tggttagtat caatgccttt aaaggcgtag aatttggggt
    11521 cggattcact tctggttcat taccaggctc agaagtgatg gatgagatcc tatgggaaga
    11581 agaaagaggc tatttccgac gtaccaataa tctaggcggc ttcgaaggcg gaatgaccaa
    11641 tggcgaacca cttgtcattc gcggggtcat gaaacccatt cctacattgt acaagccact
    11701 tcaaagtgtt gatatcgata caaaagaacc ttacaaagca agtgtagaac gttcggatag
    11761 tacagcagtt cctgcagcta gcgtcgttgc ggaaaatgtt gtagccactg tggtagcgac
    11821 agagctattg tcaaaattcc caagtgattc gcttgttgag ttgaaagctg cagtgaagga
    11881 gtaccgtgac tacttgaaac attactaaaa agggggcaaa taagatggca caacatgttt
    11941 ttatcgtggg gcttggactg attggtgctt ctatggcact ttgtattcgt cagaccaatc
    12001 cagaactgat agtagttggt tgggatagtc agtcaaaaac ccgtgagcaa gcagtagaac
    12061 agcagatcgt tgattacgtt gcaagtgatt ttgaaacagg tgcagaacaa gcagacgtta
    12121 tcttattagc cgttcccgtc cgtacaacct tagcttatct tgatgtcctt gaaagaatca
    12181 acttgtctga tcacgtcatc attacggatg tcagcagtac gaagcagcaa gtcgtgacat
    12241 ccgctgagca gaaaaatctt cggtttgtcg gtggacatcc aatggctggt tctcataaat
    12301 caggtataca ggcagcagat gctaaactat ttgaaaatgc ctattacatc tttacacctt
    12361 tggaaaagac gaaaaaagat gttgaaaaac tgcaagaact attttgcgga accaaagcaa
    12421 aatatgtaac tcttacagca agagaacatg accgtataac aggaatgcta agtcattttc
    12481 cacatattct ggcagctggc ttagtcaatc aggcagaaca attcaatcaa gagtatccgc
    12541 gagccaaaca actggcagca ggaggctttc gagatatcac taggatcgct tcctcagatc
    12601 ctgtcatgtg gacagatatt ttgttgagca ataagcagat attattggaa cgattggcag
    12661 attggcaaca agaaatgaca caaatagcag aatggatcat gactgagaac caatcggaaa
    12721 tcttttcttt tttcaatcgg gcaaaagaaa gtcgagacca gttgcctgtc cataagcaag
    12781 gcgcgattcc tgcctttttt gatttattta tcgatgtgcc agatgaacct ggcgttattg
    12841 cagaagtaac tggactgatc ggaaaagcgg gagtttcttt gattaatttg aagatccaag
    12901 agaccaggga agatatttta ggaatactgc aaatcagttt taaaaaccaa caggatttgt
    12961 tacaagcaaa aagaatcatt atgtctaata ccagttatca ttgctggatc aaataggagg
    13021 ctggataaat gcaattatta caacaaatac atggattaag agggactgtt aggataccag
    13081 cagataaatc aatttcccat cgcagcatca tgtttggagc aattgctgaa ggaacgacga
    13141 ctatacaaaa ttttttgcgc gcagaagatt gtctgagtac tttacatgcc ttccaacaat
    13201 taggtgtcga gatcgaagaa gaggaagagg tgatcaagat tcatggtcgc ggtagccact
    13261 catttgtcca accaaccgaa tcaattgaca tgggaaactc cggtacgacc atacgtttat
    13321 tgatgggtat tttggctgga cagcctttta cgactactct gttcggggat gcttcgttgt
    13381 ctaaacgtcc aatggggcga gtgatggaac ctttacgcga gatgggcgct gacttgcaag
    13441 gagaagaaaa tgaccagtat ctaccaatca ctgtgacagg aacccgctct ttatcaccta
    13501 tccgatacaa tatgcctgta gccagtgcgc aagtcaaatc tgctttgctg tttgcggcat
    13561 tacaagcaga aggcacatcc gtaatcgttg aaaaagaacg ttcccgtaac catacggaag
    13621 aaatgattcg tcaatttggc ggaaggatca cagtggaaga taaaacgatc atagtgaccg
    13681 gaccgcaaaa attaactggt caacagataa ctgttccagg tgatatttca tcagctgctt
    13741 tctttctagc agcaggactt cttgttccgg aaagccaact gttgttaaaa aatgtcgggg
    13801 tcaatccaac aagaactggt atcttagatg tgttggagga gatgggcgca gcgattaccc
    13861 agacgaatca caatgaacag aaccaatcgg ctgatttaag tgtcaagacg agtcatttga
    13921 agaaagcaac gattggtgga gaaatcattc cgcgtctgat tgatgaattg cccattctag
    13981 ctttagtggc tactcaagca gaaggaatca cgatcatcaa agacgcagaa gaattaaaag
    14041 taaaagaaac caacagaatt gatgcggtgg cagaagaatt acagaaaatg ggagcaaaga
    14101 taaaagcgac agcagacggt ctgatcatcc atgggccgac acctctgcat ggcgcaaaag
    14161 tgtccagcag aggcgatcat cgaatcggta tgatgctgca agtagctgct ttgatagctg
    14221 atggaccttg cgaactagaa ggagctgaag ctgtttcaat ctcttatccg gcattttttg
    14281 acgatttagc agaacttgtg tcaggaggag aagctcatgg ctagcatttt gctgattggt
    14341 ttcatgggcg ctggaaaaac aacaatcggt aaagggttag ctcaatgttt acaaaaagag
    14401 tatgtagatt tagatacaaa gattgaggaa catatccaat tgtcgattgc cgaatatttt
    14461 cactattacg gtgaaaaaag ctttcgaaaa gtggagtcag atattttaag aaagctgtca
    14521 aatgaagaca aaattatcgc gactggtgga gggattgtcc aaagtgcaga aaatcggcgt
    14581 tttttaaaaa cacagccgat tgttctttac ttagaggcag aagcggactg tctagttgat
    14641 cgtatccaac aagatgaaac gtctattcga cctttagctc ttggaaaaac aagagaagaa
    14701 ataaaagcat tattggcaca acgactttct tggtatgaag aaagtgcgac acatcgaatc
    14761 aacacgacca atcaaacacc agaaaagatc atcgatacca ttatagaaag gatcaaaagc
    14821 atatgaacgt cagctatcta gggccagaaa gttcattcac ttatcaggcg gcttgtcagc
    14881 tatttcctac agaacaattg actgcgtatg cttccatccc tgtttgtttg cgcgctttgt
    14941 ttcgcaaaca agtcgatctg gctgtcgttc ctgtagagaa ttcattggaa ggagctgttc
    15001 atcacacaat cgatcttctt tccaaacatc cagaagtcga agttaaaagt gaaatcgttc
    15061 ttcctatcaa gcagcagctt ttaggcaatc cagcaacaaa gattacaaaa atcctttcac
    15121 atccacaagc actagcacaa tcacaacagt ttttagaaac gcattatcct aatgttcctt
    15181 tagtagcaac agaatcaaca actgctgcag caatgtatgt agcagaacat ccgaaagaag
    15241 acgcagcagc gattgcttca ttagaaacag cacagcatgt tggattggag atcttagctg
    15301 agaatatcca agataatgag ttgaaccaga cacgtttttg gatcgtcggt gaccgaaaga
    15361 tgaccagcca gcagtcagct ccagtaaaaa tgagtgtcat cttgacacta cctgctaatc
    15421 ggccagggat gcttcataaa atgctggcag cttttggctg gcgggaaatc aacttaagta
    15481 agattgaatc ccgaccattg aaaactagct tgggagaata ttttttcgtg atcgatttgt
    15541 tgttggaccg tccaatgaca ttagtagaga atgcgctcca agaaatcaag atgctcggag
    15601 gagaaagtca aatattgggc tgttatccag tgctgacagt agaagagacg cgtagatgaa
    15661 agtcaagaat actgattgat ttatttaact taaaataaaa aaatcagcgc ttggctattc
    15721 tttatttttg atattctgat tgatttttag gaagaagcgg tagacaaatc gttacagtcg
    15781 ttcctgtccc aatcactgat tggatcgtta agccatatgc atcgccatac attaggcgta
    15841 tccgctcatc tacatggctg atgccgattc ctgaaaaatg atcatttttt tgtttggtat
    15901 tttcagtatc gaagccatcg ccgttatcaa tcacttcgaa taaaagaagc tgattttttg
    15961 ttttgccgta caccgtgatg aatccttgcg ttttattgtg aaaagcatga agataggcgt
    16021 tttcgataat tggttgaagg atcatattcg gcatagataa tgactgcagt tcatcaggaa
    16081 tcattatttt agccgagatt ctttcgccat atcgcatact caaaagatcg atgtaacttt
    16141 gtacgctttt gatttcttcc ttgactggaa tcacttcttc ttttttccca atcgtacttc
    16201 ttagtaaagt aatgaaactg tcgattcctt gaactgcttc atctgttttt ttctgccaaa
    16261 tcaaaaattt caagctagtc agcgtattat aaataaaatg gggctgtatc tgtgcttgca
    16321 tagtttggat ttcaagcgaa cgtttcttct tttcgtcttg gatcgtctgt tcataatgat
    16381 gctgagcatt acttagcaat tgattgatcg tattccctag caattggact tcatggcttc
    16441 cttgtacagg aacagtagct tttaagccat cttcctctag ccgttttagc gaatcaatca
    16501 agcggtagat cgattttgtg tgtcgatcca aatacataaa taccaatgag ccagcaaaag
    16561 caagtgtcac gatattatac aaaataatcg gaaaaatcac gcccatttgg ttagataaag
    16621 aatgaagatc gacttgactg agtaatgtac aatcctggcg gtacagagga agacgggtca
    16681 actgctgtcc atttgaaatc gttgtttcac ctactgttag ttttttgaaa atcattgatc
    16741 ggctcccaat tgtattttct tcatttgatg cgatgatcgt ctgttcttcg ttcatgaagg
    16801 agatcttcga tatctcagga tttactacag attgaaaaag attcgctagt tgcttggatg
    16861 agatcgataa aacagcatat ccgtaaacgt gattcaatgt atttttcagt tttttgacaa
    16921 agaagacgct gggtgctttc ttatcgcgaa gagtcaatcc gtggtgtgca aaaagcattt
    16981 gtgtatcagc agcacgttca ttcactctct gagcaatcgc agattgaaaa aaagaatcgg
    17041 cagatacggc agtgagcatg tcgtttccga caaaagttct tccattttct ccaaagatca
    17101 atacatcgta atcgatcatt ggatggattt ttgaaagaga gtcattcatt ttttggattg
    17161 ttcttagttg ctgaagctgt tccatatgtt gggctggatc attggaaaaa tagcttttga
    17221 cagctggatt agaatcaaaa gcggctaatg tattctcgat tgtatcattc aaactttcaa
    17281 attcagattg aatctttttt agtaccacat cattcgattg attgaacagc tggatatagg
    17341 catcccttga tcgatgataa ataaacaaag aaatgacgat ggcttgcaga aaaagaccga
    17401 tgaacaaaat gaacagaagt ttggcaaaga gttcatgctc tttgattttt tttctcattt
    17461 tacaaaagtt cctttacggt attgagaagg tgtcattcct gtctcttctt taaaaactct
    17521 ggagaaataa cccagttcgg tatagccgac tttctcacag atttccgtca aagtaagccg
    17581 tgtttccgtc aaaaagagct tggctttact gatacggact ttttttagat attttgaaaa
    17641 gttgatacca aatttttcag taaagatggc tgagagatag ctatatgaaa aatgatattt
    17701 ttcggataaa agttgtaaat tgatttcttt ctcataattt tcctggacga actcgtagat
    17761 caaagtcaag atatcgaaat tgtctttttt tgcatttgca gaaaagtggg tgagtttctc
    17821 aagtgtttct ttgacaagaa tagaaaaatc ttcgaaatac tcagcatcgg ctaataaaga
    17881 aggaagggcg atcttgagca aagaaagttc ttcattccca acatgctcgt cagccaaagc
    17941 gttgagtaat gcatagaaac gattgattgc ctcatgtttc aaataagctg gatcaatagg
    18001 ctgttccaat atcgtcgtga agaaattctc aagttctttg acagctaaag cgtagtcttt
    18061 atgaacgatt tttgttaaat aactacttgc ttgctgctcg tggttggtta ataacggaag
    18121 aagttcatgc tgataagcaa ggttctttcc cttatgatag aaacgttgtc ctttggtgca
    18181 atttctgatc tcttggaata tttcattgat atcttggatt gcaaaagaat cagaaacaac
    18241 aaaaaaatca cctggaaaaa cttcttgacg aaacttttga acaaacttcc taagtgtatc
    18301 tttggaatca gtattgatta catagccgga ttcttccgaa aaggtagaaa caggataaat
    18361 agtaaacgaa tcgtcgctat ccatcaatga atgggtgatc gactgttttt gtttagagcc
    18421 ttgttttgaa ctgaatggcc gtttttcggt atataacaga aaaacaaggt gtgttcaaaa
    18481 agaagagggt caagcaatgg acgagtttgt ccagcaatct gctgtgaaag attctcgcag
    18541 atctgctcat aacgattggt gattgggctt ttgttgggct tttttttgat tttttgtgaa
    18601 acggcagtta aggaatttag aaaaaaatca ggactcaatc gaggctttaa aagataatca
    18661 gcgacgcctt ctttaaaaga atctttaacc atatcaaatt catcatagct agagaggata
    18721 aggatctgtg tttcaggttg ttgctgtttt aatagccgcg ataattcgat tccatttaat
    18781 tcaggcatca ttacatctgt gaccacgata tcgattgcat gtaccaaaga aaaatcaagc
    18841 gctttcagcg gactggaaaa agtccctatt aattgaaacc cgtgctcaga ccaattgatc
    18901 aactgtttca ttccatctaa aatcgtctgt tcatcatcga taagaattac gcgtattggc
    18961 atttttttac ccgccttaag aatagtcttg taatttaaaa atacttattt ctaggagaaa
    19021 atgcaaggat atttttattt taaagaaacc tcaccatttc gatctttggt ctagatgcta
    19081 agattattcc ttttgtcata gttgatttgt atttttttat tattttagga gaaatttttg
    19141 aagtatctga ataataaaga aaccgttttc aaattttggt atgatgaatg tagcaaacgt
    19201 aaacaactaa tggatggaaa aggagcggat aaaaatgaac atcagaaagt taattgctgg
    19261 agtcgtttgt ttagcggcag catcaggttt cttaacagga tgcggaagtg ctagtagtac
    19321 agcagacaaa gaagaaaaag tcactgtctg ggcgtgggat gagacattca atatcaaagc
    19381 agtgaatgaa gcaaaaaaag tttatgagaa cgaagaaaca gagatagaag ttgttacgat
    19441 gtctcaggat gatatcgttc agaaactgaa caccgcttta gcgagcggga atacagaagg
    19501 attgcccaat atcgtcttga ttgaagatta taggatacaa ggctatttga cttcttatcc
    19561 agatgcattc gctaatttgt ctgctattgt aaaggaagat cattttgcac cgtataaatt
    19621 tgcggtaaac aaagtaggag ataaaatata cggtgttcct ttcgattcgg gcgtaacagc
    19681 aaacttctat cgtacggatc tgatggcaga agcaggttat acagaagagg atatggagaa
    19741 tctgaagtgg gacgagtata ttcaagtagc gcgagatgtc aaagaaaaga cgggaaagaa
    19801 aatcgctgaa gtcaatcctt ctgaccttgg tcgagtaagg atgattatgc aacaggcagg
    19861 agaatggtat acatctgaag atggggagac agtcacgatc caagacaatg catctttaaa
    19921 gtatggtctt gaattgtttg ccactctttt gaaagaagat ctagtcgaac aaacctctga
    19981 ctggaatgct ggagtaactg cgatacaatc aggagcagta gcttctagcc caactggcgc
    20041 ttggtattct tcaacgatcc aaggagcaga aaaccaatct ggaaaatgga agattgcacc
    20101 aatacctgca cttccagaaa atctgcaaaa agcgcaagct tctaaccttg gcggcgctgg
    20161 ctggtatgtg atcaaaggag tagctggaga agattcagcg aaagatttcc tagaagcaac
    20221 gtttgcttcg aatgaagaac tgatgggcac tcttgcgaaa gaaatcggac tagtatctac
    20281 gatgcttagc gcgaatgaac aagaagctta tcaagaagct tctgaatttt acagtggtca
    20341 aaagatcttt gacgattttt caacatggac atctgaaata ccagaagtga attacggaca
    20401 tgagacctat gcaattgaag cagtcgtagc tgaagctctt catcgaatca tcaatggaga
    20461 agagacagac aaggtactag cagatacaca aaaacaagta gaagcacaat tggctaattg
    20521 atcggtggta aaaaagtgaa tttcttcgct ttttcatcag acaaacgaaa agacaaaaaa
    20581 gatcacgtct gccttacaga tattgtcttt cttgtctttt catgtagata atcaatgata
    20641 gaattggagg gaaaagaatg aagcagaaag atttattcaa aaacgggcat gcttttttaa
    20701 tcttgccagt cattttgata tcaatcatgg tttttattcc gatggttttg gctttgatca
    20761 cgtcttttca aagtggtccc cctgtcaata tgacatttaa cgggttaggt aattatcagc
    20821 ggatgttgac agatgcaact tttaaaaaag ctttttttaa tacattcctg tatttacttg
    20881 tacaagtacc gatcatgttg tttctagcac tttttatttc caacctttta aatgataaaa
    20941 agttgaaata taaagggttg tttcgaaccg ctatctttct tccatgtatc acttcgatgg
    21001 tcagttattc tctgatcatg aaaagtctat tttctcaatc tggccttgta aacaacttgt
    21061 tgttgggcat tggtatgatt gacacaccga ttggctggtt gactcatcca atttgggcga
    21121 gagttctgat cattctttcg atcacttggc gttggactgg atacaatatg atttttttcc
    21181 tttcaggcat gcaaaatatt gattcggaga tttatgaagc agcatctatt gacggtgctt
    21241 ctaaatggca gcagtttatc aaagtgacga tcccaaattt aaaaccaatc attttgttta
    21301 cttccattac ttcgacgatc gggacattgc agctttttga tgaagtacaa aatattactg
    21361 gcgggggacc agcaaatgca acgaccaccc tatctcaata catctataat ttgagttata
    21421 aattcacgcc taattttgga tatgcggcag cagtttcctt tgttattgtt tcctttattg
    21481 tgatattgtc gataatccaa acgaaagtag gaggagaaag tcgatgaaaa atatggtaga
    21541 aaaagaaaca atcgaacgcc ccaacgtcaa aaaacaagca ttagaacttc gttcgacaat
    21601 tgggtcagta ttaaagtata cagcattaag catcctatca tttatttcca ttttcccttt
    21661 tgtctggatg gttttgggaa tgacgaatac acccgtggat atcacatcgg gaaaaataaa
    21721 gattggcagt aacttgatca tcaattttca aaatttgttt tccaatgagc tgaattttac
    21781 tcgttccctt tggaattcag cgatgatcgc tttggtgacc actgtgtttg cattgctgat
    21841 ttcgtcaatg gcaggatatg gatttgagat ttatcaaagt aaacgaaaag acctaatttt
    21901 caatattcta ctgctatcga tgatggttcc ttttgcggca ttgatgatcc ctttatatcg
    21961 aatgttttca caattgaacg gaacggtttt gggaatcaac agtttcttgg ttgtcatcct
    22021 gccatctgtt agcactgctt ttttgatctt cttctttcga caaaatgtta aatcttttcc
    22081 aaaagatcta gtagaagccg ctcgattaga cggtctaaaa gaactaagta ttttttttag
    22141 agtgtatatg ccgacggcta aaaataccta tgccgcggct gcaatcatca cattcatgag
    22201 tagctggaac aattatttat ggcccttagt agctttgcaa tctcctgata aacggacggt
    22261 tccgcttgtt ttatctgcta tgggtgcttc ctatacacca gactatggaa tgatcatggc
    22321 tggattagtc atcgctactt taccaactgc catcgtattt tttgttttgc aaaagcaatt
    22381 cgttcaagga atgatcggct ctgtaaaata aaaaaacaca ataagcgcgt tccttcaaag
    22441 tcatttttcc aagtaaaaag atacttttgg gacgtgtgtt attttatgtt gagaaaaatt
    22501 aaatgataac gttttaagtt tgtgctcgaa aactctttca ttttaaggta aacttaaaga
    22561 gtaaaggagg aggatacatg ctaaaaaatt ttaaagaagt gattgctgct atattgccga
    22621 tgactgtcct gattgtcatt ttaacgtttg tttttgcacc gttagaagga gaagacctcg
    22681 cttcatttct ggttggtgcg gcaatcatga tgatcggaat gacgctcttt ttgtttggag
    22741 cagattattc tatgatggaa gtcggagatc tagtagggaa atatatgatc aaaaagaaaa
    22801 gtttgaccgt tttggtcagt ttgggatttg ctatcgggat cgtgatcacg attgctgaac
    22861 cttccgtgca agttttggga caacaagtat atgatatatc tgacggagag attggcagag
    22921 tgttgctgat tgggatcgtc agcgtcggca ctggagtatt tctagccttt gcgctgcttc
    22981 gggtcgtatt caagctatcc tattatcaat tgatggctat aggctacata ggcgttttga
    23041 tcgcctcatt tttcaccagt tccgaattta tgccgattgc ttttgattcg gggggagtga
    23101 ctactggtcc agtaactgtt ccatttatct tggcccttgc cggagggatg actagtatga
    23161 tcaaacaaaa gaaaaatgaa aatgacagtt ttggaatggt cggcattgct tcgttagggc
    23221 cgattcttgc cgtgatgatt ttgggggtga tcttccaatg agtttctggc agcaagtgat
    23281 agatggaatc gaaatcgtta ccatggaagt cattttggca gtggcaccta tcgtaatcct
    23341 atttgtttta atgaatatca ttagttttcg tatgcgcaaa aagcgtttct ttgacattat
    23401 gaaaggtttc ttgataacga ctattgggtt gatccttttc ctgcacggag tcaatatggc
    23461 ttatgtaccg gtaggacagc atttaggcag tgctattgcg ggattgcctt acaattatat
    23521 actggttccc cttggctttt taatggggtt cttagtaggg tttgctgagc cggcgattca
    23581 tgtaatgatc aaacaggttg aagaattgag cgaggggaga atccgatcga aaatcatgct
    23641 gatggtaatt tcaattggga tcggcgcagc agtaggattg tctatgtggc gtttgctcgt
    23701 cggattctct ctttattatt tcttgatccc aggatacggg ctagcattta ttttaggacg
    23761 aaaagtggac aagatctttt tagcaatggc ttttgataac ggtggagtag caacgggccc
    23821 gatgtgttcg acatttattt tatcaatgag cgtggcaatc gctgcacaaa ttgaaggaag
    23881 agatccgatc attgacgggt ttggtgtggt tgcaatgatt gcattgacac caatattatc
    23941 cactttagta ctaggatata tttataaacg aaaagatgaa aaagacagaa tcgaaaaaga
    24001 aagattgcaa aaattggggg aaagcaaatg aaagatcttg tagcattgaa tcttcacatg
    24061 attgtgacga tcgttgacgg aggaatgggc gaagatgtta tcgacttttc aaaagaagca
    24121 ggtgcagcag gcggaacgat cttacatgga agaggttctg gtgtacatga ttcagggaaa
    24181 tttttaggtt tggacatttc gccagaaaaa gatattgtgt tgacattagt acccgatagt
    24241 ttgacgaata aagtaatgga tgcgattggt caaggcatca acatcgatat tccaggtaat
    24301 ggtatttgtt ttagtattga tgtagacaaa gtcattggta tcactaagat tcatcaatat
    24361 cgagaaatta tcggaatgca agaactttta gatgaaaata acgatagaga aaagtctgaa
    24421 tcatagacaa gcgtttgaag aggaaataaa cacaaacaag aaggcggatg agcgttattt
    24481 ccgctttttt gtttgtattt ttctcgtgaa tacttatgaa atagtaaatt tttatgaaat
    24541 tctactctaa gcatcagact ttcttttcaa aatatgctat tttaaaaatg atgaaaaaag
    24601 ggggagaaaa atgaattttt tagttgtttt aaaagatact ctgattgagc gatctacatt
    24661 tctatatgtt caattgcagg aaaataaaac attgctgcat ttttcaccag agttccatct
    24721 agaggggcta acattaggag aaatctatca agcagaaggg ctgtcggctg ttttgcactt
    24781 ttttaaggta gaattggaat taccagtaaa agattatatc gagctttctc ttgaatcttg
    24841 ggatcgagca gtggttgaac tgtttccaga aggtttaatg atcgatacaa acgatgggaa
    24901 gatccatctg actgcagcag aactgcaaaa acaaatgcgt tatcaaatag atgacgaaaa
    24961 ttctttttct atttttagac gtcagcaaaa gattctaaaa agctttttaa aagtgatcag
    25021 taaaaaacgg aatctgatca aaacgacaca gctgttgaaa aaatatccag gtctgcttca
    25081 cacgtctatt caatttcctc aattgattac gatgatccat cgttttatac gtatgcaaca
    25141 gctaccatct aagaagctgt ttcttccatt gacggacaca tttcgagtag tcaacagaga
    25201 agataaaaag aaagtgattg tgattgactt taccagaaat cggaatgtcc ttcaccaagt
    25261 tgcaatggaa aaggccaatt aaggcttttt ctttttggat ttttttgtca taataaatat
    25321 tagatgatgg aggagataat atgatgcaaa catggttaga aaggctaata cgatttcagc
    25381 agaactatca caaacttatg cagggaagat atgcgaaatt tgatcagttg aatcgcggtc
    25441 tattgatcgc tgcgcttgtc tttagtttgc taagtcgttg gttgccctat agaatcggac
    25501 aattgttgtt catcgtcttt ttcggatgga tgatatttcg tttcctatca aaaaaaatct
    25561 accccagact aaatgagaat caacgttatt tgaaatatct tgatagagtc aaagaaaaat
    25621 atcatcagat aaaaaacaaa aagcaagtca aaatggatcg aaaaagttat actttttttg
    25681 agtgcccgaa ttgtcatcaa aagcagcgtg cacctaaagg aaaaggacga atacgtgtga
    25741 catgcaaaac atgtggtata aaattcgaaa cgaatgttta agggagaaaa gaatgaaatt
    25801 agtattatta cgtcatggcg aaagcgaagc aaattttgaa aattactgga caggatggct
    25861 agatgtcgcc ttaacagaaa aaggaaaaga acaagcagca gttgccggtc aaaaaatgct
    25921 tgaagccgga cttcaattcg atgccgttta tacctctgtt ttgaaacgtg cgattcttac
    25981 cggacaagtc gcactagaag aaatgggtca gttatggctt cctataataa aaacatggcg
    26041 tctaaatgaa cggcattatg gggatcttgt gggtaaaaac aaagataaga tgaaagaaat
    26101 atacggaaaa gatcaagtaa aaacatggcg cagaggatat acggaagttc cgccgttaac
    26161 cgcagaaaat agatttgacc gcagatatga tcacttggat ccgcatttga tcccatacgg
    26221 agaaagtctg gaaatgacag tgaatcggat cattccctta tggcaagatc atctagcacc
    26281 aaaactaaaa gatcggcaag atttactagt tgtgggtcat ggtaatagca tccgagcact
    26341 gacaaaatat ttagaagatg ttcctgaaga tcaaatggat acgatcgata tcccaaatgc
    26401 acaaccaatc cagtatacat tagcagatga tttgacaatt ctggacaaaa aaatacttta
    26461 aagaacacct ttatctaaaa gtgagagata gaaaaaagaa gcaaaggaat ggaacaaaag
    26521 gaacatgaaa aattatatat gggactttga tggtacattg tttgatacgt atcccgcaat
    26581 ggtagatggt gcatggcaag cactaaaaga ttttggtatc agtatggaca aaaaagaaat
    26641 ctacttcaaa atgaaaaagt attctactag ttatttgatc aatgaatcaa acttgaatgc
    26701 cagagagttt aatgaattat ttcaccgata tgaaaaagaa agtacggaag tttcccgtcc
    26761 atttccagaa acgaaacaag tactggaaat gctaaaagac aacggcggtc gtcatttcat
    26821 cctgacccac cgtttgacag aatcaacatg gggcctgtta aaagaacatc gattggcaca
    26881 tctgattgag gaagtagtag ggatcgatca ggactttcct cgtaaacctg atccagcatc
    26941 tttaaattat ttgatcgata cttttcattt agaaagaaca gacacgatga tgatcggcga
    27001 tcgtcgttta gatatcgaag cagggaaaaa tgcaggagtc gctacttgtc tttacgacat
    27061 cgatcatttt ctgggtgaga tcccggcaga ttatgtggta ggaaatctga atgaaatcct
    27121 taccttgcag agcatcatta aaaagtagta tcccctttca aaggagatat gttataattt
    27181 tcataaagga gtgataaaat tatatggaaa tcagaagaca aaaagaagtt gtagaagcat
    27241 ttcattatga tatgagattg ccgaaccaag aagtagaaac agatttgaaa gtaggcttct
    27301 cgcctttaaa agaaacgatc gaaaactatc cgaaagaaca tagcatcgta ggagcacgct
    27361 tagagttccg tttagtgttt gatacatatg ttttatctgg aagtgtgagt cagatcaatc
    27421 atatcctcaa tcggaaaatt gaaggtcaaa atgatgtcac acaagaagaa gtggacgagc
    27481 tagtagcacc gttattcgat atcgtacgcc gaatgtctta tgaggtgaca gagatcgcct
    27541 tagatcagcc tggggtaaaa ttgaattttc aatcagaaaa acaagaatag acagtaaaaa
    27601 catacctgcg atagattaaa gctatcacag gtatgttttt ttattactaa ttgtaagcaa
    27661 aaagaatgtc atttcgataa aaatcgtcta aaatataaaa gtcactgaaa aaaatttttt
    27721 caggaggatg gttgaagaat cattctcggg aacctttact tccttcgatg gacttgtcat
    27781 taataatatg aggaggagaa aaatgacaac actcatcaca gttttagtgg ttctagtttt
    27841 tgcggcatta ttatatgtgt tctatcgtat gcaaaagcgc catacaaaat tttcaacacg
    27901 cgtctttaca gcattaggag ttggtatcgt ctttggcggt ctgcttcaat tgatctttgg
    27961 tgtagacgac aaagtcgtta ctcaatcaat ggaatggatc gggattgtag gtcaaggata
    28021 tgtatcattc ttgcaaatgc tagttatgcc tttagtgttt atttcgatca ttggtgcatt
    28081 cacaaaaatg aaagtttctg aaaagctagg gaaaattagt gcgactgtat tgacttcttt
    28141 acttggaaca acagctattg cagcattgat cggaattggt tcaacaatgc tgttcggact
    28201 ccaaggagct aaattcacac aaggagctgc agaaacagca cgaatctctg aactggcaac
    28261 acgtcaggaa acagttgaaa acttatcgat tccacagcag attttatcct ttatccctaa
    28321 aaacgttttt gctgatttag caggtagtcg tgcaacaagt acgattggag tcgttatttt
    28381 cgcggcattt gtcggaatcg cttatctagg cgtgaaaaga aaagcgccaa aagaagcagc
    28441 attcttttca gatttagtcg acagtctgta taaaatcatt atgcggatcg tgacgcttgt
    28501 tttgcgtttg acaccttatg gagtatttgc tttgatgaca aacgttttgg caacaagtga
    28561 ttttgcagct atcgtcaatt taggtaaatt tgtcgttgct tcttacgtag cattgcttgc
    28621 tgttttgatt gtccactcac tgattttgat tggtgcaaaa gtcaatccaa tcaactattt
    28681 caaaaaagcc tttccggtat tgagctttgc gtttacttca cgttcaagtg ctggagcttt
    28741 gccattgaat atcgagactc agacaaaagc tttaggagta gatgatgcaa cagctaattt
    28801 ctcagcaagt tttggtttgt ctattggaca aaacggctgt gctggcgttt atccagctat
    28861 gttggcaacg atcgtcgcac cagcagctgg agtgaatgtc tttagtttgg aatttatttt
    28921 gatgcttgtg gcaattgtaa caatcagttc ttttggtgta gctggagtcg gtgggggcgc
    28981 aacatttgct tcactgatcg ttttaggggc aatgaacttg ccagtagcaa tcgttgggtt
    29041 agttatctct gttgaaccat taatcgatat ggcacgtaca gcagtaaacg tcagcggtag
    29101 catggtagct ggtatcgtga caagtgcacg catcaaagat ttagatcgcg atgtcatcaa
    29161 tgatgattct aaagtgatcg atgtgaatgc atagtcaaaa agaggttgtg atagaagtgg
    29221 tcagctttaa gaaataagaa gaagtttcct aaaaatagtt tctcaaattt ttgtgaaatt
    29281 tcggcttatt tccgagaagc tacttctgaa acaccgtgaa taagaggttg tgaaaaagct
    29341 gccctgcatc aagtaataag aacagccaaa caaaaatagt tcctcatatt ttacgtttgg
    29401 ttgagcttat taccgcagat gcaagctttt gaacaccgtt taagaaagag gtcgtgagcc
    29461 tgccgtttca ctctgagtca caaagagcaa tttcaaaaaa tagcttctca tgtttgttga
    29521 atattgtgtc ttgtgaccga ggagttgtca ggagaacacc gtttaccaga ggttgtgact
    29581 tgtttttagt cacgacctct ttttttattt attccatata aatacacatt atttattccg
    29641 aaaagtgaaa gcgcaaataa caaaaacaac caacttattt attattaaag aaaagcgtat
    29701 ttaagtagga cgattctgtt gagagctttc gtagattttc ccattttcct atggtaaact
    29761 gacaaagttg agaatgttag aacagatcgt ttaggaggaa acagatgttt acgaatgaaa
    29821 taaaaatcat gctttatgtg gacgatgtag aaaaaaatgc aacgttttgg agagcagttg
    29881 gctttgcgga aaaagatcgt caagaaatgg atgggacgct tataattgaa gtaggagttg
    29941 aggacagtca aacctcattt ctgttatatg atcgagcatt tgttgagtcc cattcacctg
    30001 gcacagctac tagtgcccca tctttgatgt tttacagtaa agatatcttt ggactatata
    30061 aaaaaatgca gacgcaatca gtcacattag gtgatatggt acagcttgga gaagaatatg
    30121 tctttaactt tgctgatccc gatggcaact actttgcggt tacaggtaaa taatagaaat
    30181 aaagactttg tttttctgtg cgaaacagga aagcgaagtc tttttaattc aaataataat
    30241 attatgtaaa caaaaaactt tccatgcgta attatacaca agcaaataaa tcgtcaaatg
    30301 cgaacgcctt ctttatactg tttaggaacg aaaagaagga aggaagaaat acatggatta
    30361 tcaagtactg ctttattaca attatacaaa aatcgaagaa ccagaggaat ttgccaaaaa
    30421 tcatttagcc ttttgcaaat ctttggatct taaaggtcgt attctagtag ctgaagaagg
    30481 aataaatggt actgtgtctg gaaccgttga aaacacgact gcctatatga aagcaatgaa
    30541 agctgatcct cgatttgctg atacgatgtt caagatcgat ccaagcgaag gacatgcctt
    30601 taagaagatg tttgtaagat accgtccaga gcttgtaagt ttaaagctag aagaggacat
    30661 tgatccaaac caagtgacag gtgcctatct ttcaccgcaa gaatttaaag aagctatttt
    30721 agatgaacag acagtggtga ttgatgcacg gaatgattat gaatatgact tgggacattt
    30781 tcgaggggct gtacgcccag atatacgcag ttttcgagag cttccagact ggatccgcga
    30841 gcacaaagaa gaatttatgg aaaaacgggt agtgacctac tgtactggtg gcattcgttg
    30901 cgagaagttt tctggttggc ttgtccgtga aggattcaaa gatgtcggac agctgcatgg
    30961 cggtattgct acttatggta aggatcctga agtccaagga gaattatggg acggcaagat
    31021 gtatgtgttt gatgaacgga tcgctgtcga tatcaaccaa gtagataaac aaatcgtcgg
    31081 tcgcgactgg tttgacggaa caccatgtga acgatatatc aattgtagca atcctgagtg
    31141 caatcgtcag attcttgctt ccattgaaaa tgaagaaaaa catttaggag catgtagcat
    31201 agagtgtgcg aaacatccgc gcaatcgcta tgttcaagac catggaatct ctccagaaga
    31261 agcagaactg cggtttacag ctttaattaa atagaaaaca atagttttga ttgtcgacca
    31321 tttccaatat gcgctatact agcgttgagg atggtgatcc acatgaaatc attgatttat
    31381 gctattgggg atgtccatgg caaacatgag ctttttgaaa aacttattca ctattatgat
    31441 ccaaagatcc atcagcttgt tttattaggg gatctcaatg atcgaggtcc tcgtacaaaa
    31501 gaatgttttt tattaggaaa gcaattagtc gaagaaacag gagccattta cctccgggga
    31561 aaccatgaag aatatttttt gcaatttctg caatcaccgg aggactggta tgcgccttat
    31621 atgagaaatg gtggaaaaga aacgtttgaa agcctgattc acccaggggc atcggaggaa
    31681 tactccccaa ctgaaatctc gatgatgatc caatcgagat attcagaact gattaaattt
    31741 ttagtccagc gccccttgta ttatgaatgg gaaaattatc tttttgtcca tgccggtgtg
    31801 gatttgacaa agaaagactg gcgtaagaca agtctaagag actttatttg gataagggaa
    31861 ccatttcatg aagggaaaaa caatacaggt aagacaattg tatttggaca tacgattaca
    31921 ccaatgctcc atggagatat gcagacaaca gacctctgga ttcaggatca taaaatcgga
    31981 atggacggtg gggctgtatt cggagggtca ttgcatgggg ttgtttttga caaggaaggt
    32041 attcttcaag atattgaata tcagaacaac gaaggacctt ggcatggaat ctgactaatg
    32101 gaaagaatga aggagctgtg actttgttgc ggctcttttt atttttatag agaatattgt
    32161 ttctttcagt agtttaaccc aggataaaaa gtatgataga ataaagaagc aatttttagc
    32221 agaaaagagg cattttaatg acggaaaatt tgaaccagac gatcattcag cttgtacaaa
    32281 aagaactttc agactatcgt cctaagcagt tgactactgt acttaatttg ttaaatgaag
    32341 gaaacactgt tccttttatc gcgcgttacc gaaaagaaat gactggaagt cttgatgaag
    32401 tccagattcg tgaaatcgaa gagcggtatg cctatcttga aaacttagaa aaacgtaaaa
    32461 ctgaagtgat tcggctaatc gatgaacaag ggaaactgac tcccgagtta gaagcagaaa
    32521 tcacccaagc cgtgaaaatg caacaagtgg aagatttgta tcgtccatac aaacaaaaac
    32581 gacggacaaa agcaacgatt gctaaagaaa aaggacttga accactcgcg aagtggctga
    32641 tgcagcttac agatggagag gtgcaatctg aagcagaaaa atatattgac aaagaaaaag
    32701 aagtctcttc tgccgaagaa gcgttgcacg gtgctcatga gattattgct gaacaagtca
    32761 gcgataatgc aaaatttcgg acttggattc ggtcttatac gtataacaaa ggtatgtatg
    32821 tcagcaatgt caaggatgag caagcagatg aaaaaggcgt atatgaaatg tattatgact
    32881 ttgcagaacc cgtccataaa atggtttctc atcgaatatt agctactaat cgtggagaaa
    32941 aagaagatat tctgaaagta ttctttcaag tagatgaagc agcaatttta gcttacttgg
    33001 atcgccaaat agtcaaaaat cctgcctctc cttcttcttc attcgtcaga gaagcctatc
    33061 aggatagtta taaacgcttt attcagccag caatcgagcg agaactgcgc aatgaattga
    33121 ctgaaaaggc agatgagcaa gcaatcgcta ttttcggcga aaacctccgt aatcttttgt
    33181 tgcaaccgcc actaaaaggt aaagttgtcc tgggatttga tccggcttat cgtactggat
    33241 gtaaactagc agtcgtagat gcgacaggga aagttttagc aatcgaagtt atttatcctc
    33301 ataaacctgc agcgcaagca aaaagagaag ctgctggacc agcttttatc caattaatta
    33361 ataaatatca agtagatatg gtagctatcg gaaatggtac agccagtcgg gaatctgaac
    33421 tttttgtggc tgagcagttg aaatccgccg accataaaac ctattacgct atcgtcaacg
    33481 aagcaggcgc ttctgtttac tccgctagcg aaattgcacg aaaagaattt ccgcatctac
    33541 aagttgaaga aagaagtgct gtttctattg cccgtcgcct gcaagatcct ttagctgaat
    33601 tagtcaaaat cgatcctaaa gctgtcggag taggccagta ccaacatgac gtttctcaaa
    33661 aacgtttggc tgaacaattg gattttgttg tggaaacagc ggttaaccaa gtaggtgtgg
    33721 atgtgaatac agcaagcccg cagctcttgc agcatatatc aggattgaat aaaacaactg
    33781 cccaaaacat tgtcatttat cgtgaagaaa acggagaatt caccgcacga actcaattga
    33841 aaaaagtgcc acgcttagga ccaaaagcat atgaacaagc aatcggattc ttgcgagtcc
    33901 ctggtgggaa aaacattttg gacaacacag gtatccatcc cgaaagttat tctattgcaa
    33961 aagaaatatt aacttcagtc caactttcag aaaaagagct tggtacagaa gaagcagtag
    34021 aaaaattgac acgattatcc gtagagaaac tggctgaaag cctatcagtc ggagaagaaa
    34081 cgttagccga tattttagct gggctgacac aacctggacg agatatgcgt gatgagatgc
    34141 cagcgccctt attacgtaca gatgtattga gtatggaaga cttgaaacca gggatggaat
    34201 tgactggaac ggtccgtaat gtcattgact tcggcgcatt tgttgatatc ggtgtgaagc
    34261 aagacggact tgtccatata tcgaaactca gcaaaaaatt cgtcaagcat cctacagatg
    34321 tcgtgtcagt aggagatatc gtaacagttt ggatagaaca agtcgatacg aaaaaaggta
    34381 gaatcagttt gacgatgctt tccccttatg aagagtaaca atgaggagta acaaaaattg
    34441 acacaagaag aactgcagca gttagtagaa gagatatcat taaaaagctt ccagcttcct
    34501 ttcaagcacc aagcaatgtt caatccacgc ttacgtacaa ctggcgggcg ctatcattta
    34561 aacgatcatc atttagattt taatgcgaag ctatttgaag agtcagacga agagacgatt
    34621 gcaggaatca tcaaacatga actttgccat tatcatcttc atctagctgg aagagggtat
    34681 cagcataaag atatggattt caagtatctt ttgaaaaaaa caggagggtt gcgctatgca
    34741 ccagccttga cgaagaaaca gataaagatg gagtattatc gatgccaaga ttgttctgcc
    34801 acgatctgcc gcaagagaag aatcgatgta cgcaaatatc gttgtggaag atgtcatgga
    34861 gaaatcaaat ggattgagac aaaaagagaa gactttaatg aagcacaaaa aatcagatga
    34921 aaggatagaa ggcctttgga aaaaagtgag cagaggaatc taaaagactg gaatcagcta
    34981 ttaaattttt tagccgcacc tgaagaggga aattttgagg aactgacggc acctacactt
    35041 attttagctg gaaactgtct tcctatctta gctgataaag cagcacaaat gtatttaaat
    35101 ggccaagtag atcaattgtt tttggtcgga ggggtggggc atgcgactag aatcctttat
    35161 gagaattttg aaaagcaagg atttcatttt gaagatggca tgagtgaaag tgaaatctgt
    35221 cgtcagtatc taaaagaagt ctatgatttg cccgataagg cttttctgat agaatcgaag
    35281 tctacaaatt ctggagagaa cgccactttt tcactggaga ttcttcattc cttaggagct
    35341 gtaccagaaa aaatcttatt gatgaatgat ccgacactgc aacgcaggac tcgagcaact
    35401 tttgagaaag tatggcaaaa tgaacaaact attttcgcga atgctgtgcc atttgtgccg
    35461 gaaatccttc acttttctga agagatcatc tttacagcaa aagaactgaa tcatcaatgg
    35521 cctaaggaat atttccatgc gctagtatta ggagaaatgg aacgtctcca tgacgatgag
    35581 aacggctatg gtccgaaagg aaaagatttt attcctcaca ttgatatatc agaggaagtt
    35641 tggtcttctt acaccagaat taaagagtca ataaaaacgg atttttctcg tacttgacca
    35701 ttttagcaag cgaatcctcc tttccaaata aatttggttg aaattttcaa tcgtttataa
    35761 gataatgagg agcgtgttga atgtaatagc taaaagaatg gacgctagat aagagaagag
    35821 gattcaaatg gaaaatcaaa ataaattgta tcattttgtt ggtatcaaag gttcaggaat
    35881 gagctccttg gctcttgttt tacatgaaca aggtctaaat gtccaaggat cagacattga
    35941 aaaatatttc tttacacaaa gagatttaga aaaagcaaat atcacgattc tgccatttaa
    36001 cgcagataat gtaaaaccag ggatgacgat cattgcagga aatgcatttc ctgattcaca
    36061 tgaagaaatc cagcgagcaa aagaattagg tttggaagtg attcgctatc acgactttat
    36121 tggtcatttt atccaaaatt atacgagtat cgctgtaaca ggctctcacg gaaaaacaag
    36181 tacgactgga ctgctctctc atgtattgag tggtgtccgt ccaacaagtt atctaattgg
    36241 agatggaaca ggacatggtg atccgcaagc ggaattcttt tcatttgagg cctgtgaata
    36301 tcgccgtcat ttccttgcct attcaccaga ctatgcaatc atgaccaata tcgattttga
    36361 tcatccggat tattatacaa gtatcgacga tgtatttaca gctttccaaa caatggctgg
    36421 acaagtgaaa aaagctattt ttgcgtatgg tgacgatgct tatttacgca aattaaaagc
    36481 aaatgtgccg atttattatt atggtgtcac agaaaacgat gatatccaag cacgaaacat
    36541 cgaacgcaca acttctggtt ctgcttttga tgtctatcat ggggatgaat ttgttgggca
    36601 cttcactgta ccagcattcg gtaaacacaa tatcttaaat gctttaggtg ttattgcagt
    36661 agcttatttc gaaaaactgg atctgaaaga agttgcagaa gaaatgctga ctttcccagg
    36721 agtaaaacgt cgattcagtg aaaagatcgt agcagacatg accgtagtcg atgattatgc
    36781 gcatcaccct gcagagatca aagcaacgat tgatggcgca cgccaaaaat atccagataa
    36841 agaaataatt gcagttttcc aaccccatac gtttacgcgt acgattgcat tgatggatga
    36901 atttgcagaa gcacttgatt tagctgataa agtttactta tgcgatattt ttggttcggc
    36961 tcgggaagaa caaggaaacg ttaagattga agaccttgga gcgaaaatca aaaaaggtgg
    37021 agaagtcatc aaagaaaata atgtatctcc attgttagat tatcatgatg cagtagtaat
    37081 cttcatgggt gctggagatg ttcaaaaatt tgaacaagcg tatgaaaaac ttttaagcag
    37141 cacgacgaaa aatgtactgt aatacaatta ctgcagtgtc cttataaaaa tagaaagagg
    37201 gggagtggca aaaatcttgc caagccccct tttttgcaac ttatgataaa cggtgttcaa
    37261 aagcttgcat ctgcggtaat aagcccaatc aaatcaaaaa catgagaagc tgttttttct
    37321 tggctgttct tattatttga tgcaggacag ctttttcacg acctctgtaa actataggaa
    37381 tctgttaaat caagggagca gaacttgcct tttacgctac ttttggtaca ataatgtatg
    37441 attaaaaata aactggaggg tttctatctt gaacataggt aagccgagaa aactaggaaa
    37501 aacaataaat gaaatcgagg aaggcgactc gttatcctta acagaatcta ttgaagataa
    37561 ccagcttttg ctgtatttgg gattgacgaa tgatgcaaat cctttgtata tccaacatga
    37621 ctttgcacaa aaaacagaat atcaacagcc aatcgttcca tcagtgatgc tgatggggat
    37681 catcacaagt gcaatttcaa agcatcttcc tggaccagga tcacatgttg tcaatttttc
    37741 tgtcaatttt attgaaccgg ttttccatta tgaaacactg acttttcagt ttgaggtcat
    37801 aaaagtagat aagatgaagg atgtggtgac gatttctgta gaagcggaga atacgaacga
    37861 agatcgtgtg ttggatgctg tcgttatggt ccaaccgcca caagttcctg aattggaaat
    37921 agcaatcgaa agtgaaggtg aattgaatga ataatccaag tacatcaatg tcaggtatca
    37981 atcgttttta tgcaaaagtt tatggatttt taggtatggg acttgccctt agtgcagtca
    38041 cttcttactt ggctctttgg gtatttcctt tgcaattagc aacttttatc aataattttc
    38101 cactaggctt tgtgggtctg tggatcgttg aactgatttt agtaatggtt ctaggcgtaa
    38161 aagcacaaaa gaatccgagc ttagcaatcg gtggatttat tgcctactct cttttgaatg
    38221 gcataacatt agcggttact cttgcatact acgatattgg aacagtaacg caagctttct
    38281 tgacagctac tttgatgttc atggggatgt ctgttgtcgg agtaacaaca aaaagagatc
    38341 ttagcggggt tggaagagca ggacaggcag ctttaatcgg agtgatcatt gcactagtct
    38401 tgaacatctt cattttgaga agtagcgtag ttgagctgtt catctcccta ctgttagtcg
    38461 tcatctttgc agggattaca gcttatgata accaaagcat ccgtaatatc tatgtccaga
    38521 caaacggaac agcacatagt ggaattgctg tattcatggc attgaagctg tatttggact
    38581 ttatcaactt gttcttggcg tttctgagaa tctttggacg gaacaactaa aaaagaaaaa
    38641 aattcatcag ttggggctat accgaattat ttggtatggt cccgcttttt cttttcgcat
    38701 tccgcaggat tctttgttag aatgaataag aactttacag taaggagtta agaagatgac
    38761 aaaaaataaa ttattactcg ttgatgggaa cagtgtggct tttcgcgcat tctttgcgct
    38821 tcacaattct cttgaacgtt tcaaaaatag aaacggtctt catacaaatg caatctatgc
    38881 attcaatacg atgtttgaaa atgttatgca aaaagagcaa ccgactcatg ttctggttgc
    38941 gttcgatgca ggaaaaacga cttttcggac agaaatgtat gcggagtaca aaggtggaag
    39001 atccaaaaca cctggcgaat tcaaagaaca aatgccatat atacgtgatt tgttgacagg
    39061 actaggggta cagtattacg aattgcccaa ttatgaagca gatgatatta tcggtacttt
    39121 ggcagaaaaa gtagacaaag atcaatttga tgtcgtcgtg ctttcaggtg atcgggattt
    39181 gactcaatta gctagtaaag aagtaaaagt ggatatcact gtaaaaggtg tcagcgatat
    39241 cgaaagctat acaccagaac atgtagcgga aaaatatgat gggttgacgc caaaacagat
    39301 tattgacatg aagggactag ccggagatgc ctctgacaat atccccggtg tgactaaaat
    39361 cggtgaaaaa acagctatca agctattgaa acaatatggc tcagtcgaag gaatttatga
    39421 acatatcgat gaaatgaagc aaagcaaaat gaaagaaaat ctgataaatg acaaagagca
    39481 ggctttctta tcgaaaaaat tggcgaccat caatacttca gcgcctgtgg atgtctctat
    39541 cgactcattg aagtatgaag ggaaaaatct cgataaactc gttccgtttt ataaagaaat
    39601 ggatttcaat cagttccttt ctaaattgaa tatcgtagaa gaagaagtaa aaatggatga
    39661 tattttgttt gaagtcgttc atgaattcaa agaggaaatg tttactacgg atatggcgtt
    39721 gtacgtggaa atgatgggcg acaattatca tacagaggaa atcgtcggag tagcgtgggg
    39781 gacagaaaag aaaatttatg tcacgaacga tctatctgta ttcgaaaacc gagcgttcca
    39841 ttcttggatc actgatccga ctcggctgaa aaaagtatat gatgcgaaac gaacttatgt
    39901 tgctttaaac cgctatactg gaaaaacgaa aggaatcgat tttgatgtct tgctaggtgc
    39961 ataccttctc gatacgaatg aaaaaagcac agatatcgaa ggaatagctg ctcattacgg
    40021 atacaacgat atccaatcag atgaagctgt atacggcaaa ggggcgaaaa aaggtttacc
    40081 agaagaagag gaactctttt ttgctcattt agcgcgaaaa gtagctgcaa tcaatgcgtt
    40141 gacaccaaaa ttgagccaag aactggcaga aaaaaaccaa gaagatcttt tccggaaaat
    40201 ggaactgcca ttgtctatca ttttggcaga gatggaaata agcgggatca aagtagatgc
    40261 gactcgctta caagaaatga agggcgagtt ctctgctcgc ttaagagaga tcgaacaaaa
    40321 aatctacgaa gaagccggcg aagaattcaa tttgaattca ccaaagcaat taggagtgat
    40381 cttatttgaa aaaatgggat tacccgttat caaaaaaaca aaaactggat attccacagc
    40441 cgtagatgta ctggaacagc ttcgtgaaca agcaccaatc gtagaggata tcctcaccta
    40501 tcgtcaaatt gcaaaaattc aatctaccta tgtcgaggga ctgctgaaag tgatccaatc
    40561 agatggaaaa gtgcataccc gttatgtcca gacattgacc caaacaggac gattaagctc
    40621 tgttgatcct aatttgcaga acatcccgat tcgattagat gaagggagaa agatacgtca
    40681 ggcatttgtt cctagagaaa aagactggct gatttattct tctgactatt cacaaatcga
    40741 gttacgcgta cttgcgcata tctcaaatga tgaacatttg aaagctgcct tcctagaagg
    40801 tcaagatatc catgccagca cagcgatgcg tgtatttgga atcgaaaagg cagaggatgt
    40861 cacaccaaat atgcgtaggc aggcaaaagc agtgaatttt ggtatcgtgt atggcatctc
    40921 tgattacgga ctttcacaaa atctaggaat cagccgaaaa gcagcacagc aatacatcga
    40981 tacgtacttt gaaaaatacc caggtgtcaa agaatatatg gaaagaatcg tacgcgaagc
    41041 aaaagaccaa ggctacgtcg aaacgctgta tcaccgccgt cgatatctgc cagacatcaa
    41101 ttcaagaaat tacaatatcc gttcttttgc ggaacgaaca gctatcaata caccaatcca
    41161 aggaagtgct gctgatattt tgaaaatcgc gatgatcgaa ttggataaac gattaaaaga
    41221 aacaggtctt caagctacga tgctgttaca agttcatgat gaacttgtct tcgaggtacc
    41281 ggaaaaagaa cttgaatcat tagataaatt ggtgaaggaa gttatggaac aagctgtttc
    41341 tcttcatgta ccactgatca ctgacagcag ttggggaaaa acgtggtacg aagcaaaata
    41401 aacattgttt aagacagagc aatataatga aggtgaaaag aaaaaatgcc agaattacca
    41461 gaagtagaaa ccgttcgtaa aggattggaa cggttagtag taggaaaaac gattcaaaaa
    41521 gttcaggtct tgtggcccaa gatcatcgag caaccagaga cacccatctt tgaggcttct
    41581 cttgttggcg aaaccatcca atccattgga cgtagaggga agtttttaat ttttcatctc
    41641 gatcattgtg aactgatctc acacttgcga atggaaggaa aatatcaatt tacgaaagaa
    41701 aatacaccaa tcgataaaca tacgcatgta cttttctttt ttgaggatgg aagccaactt
    41761 cgctacaacg atgtacgaaa atttggtcgt atgacaatcg tcgaaaaagg agcaagtgca
    41821 acttaccgag gaatcatgaa attgggacct gagcctttgc cagatagctt cttattagca
    41881 gatttcgcaa atggactgaa aaagtcccac aaagcaatca agccattgct gctagaccaa
    41941 cgtctagtga ctggtctagg taatatttat gtagatgaag cattatggga agcgaagatc
    42001 catccagaac aaccggcgaa cacactgcgc cccaaagagg tagaacaatt acgtctatcg
    42061 atcattgatg ttttggatcg tgcaatagaa gccggaggta ccacgattcg cagttatttg
    42121 aatgcattag gagaatcagg cggatttcaa gtagcactgc atgtctatca gcaaactggc
    42181 aaaccatgta tacgttgtgg aacgccgatc gtgaaaacaa aagtggcaca aagggggacg
    42241 cactattgtc cgaattgtca aaggctaaaa atcgcaaagt aggatttgtt ttaggtttga
    42301 cgggtggaat agctaccggt aaaagtacgg cagccaaagt ctttcagtct catggatttc
    42361 ctttgatcga tggcgatgtg attgccagag aaactgttga gcctggaacg cttgctctga
    42421 ggaaaattgt atcagtgttt ggccaagagg tattgcagcc tgatgggcaa ctagatcgag
    42481 ggaaacttgg gatgattgtt tttccatcaa aggaaaaacg gcaaaaactt gatcagctat
    42541 tagatccgtt tatccgaaaa gcgataaaag atcaaatcgc ctctctaagt tccaatcatc
    42601 ctttagtaat tgtagatatc ccattgctct acgaaggtca ttatgaccac tatatggatg
    42661 cagtagctgt cgtttatact acgccagaaa ctcagcttca gcgtttgatg aaacgtaatc
    42721 aactgacgct agaacaagcg cagcagagaa ttagtagtca acttccaatc gaagaaaaaa
    42781 agcagcgtgc ggatattttg ttcgacaaca atggaacaaa agaaaaactt gtgggacaaa
    42841 tcgaaaattg gctttttgct aatcgctttc tttgagtatc tgtgtcaatt ttgctatcct
    42901 gagtatatag aaagtgaggg atagcgatgg gatcattaaa aaatcaaaag gataaagtcg
    42961 taggtaacgc aaaagaaaaa atcggcgaga tgctcaataa agaaaatcta atcgatgaag
    43021 gtcgtacgca atcagaaaca gcagaagaaa aagaaaagca gtaccaagca agaatcgatg
    43081 aaatagaaga ccagcaggca gaagaagaag cgaagcggca agatttgact caagaacgaa
    43141 aaaatccgac acctgatcgt agtaaaacac cgctcccaga aagtgaccac attcccgatg
    43201 agcaaaaaat cgaggaacag cgaatgaaac actatacagg aatcgaagag aagttataaa
    43261 atagtaaaaa atcgagatgt gacttgaagc agatgagaag gtgttcatct gcttcaagtt
    43321 ttttgtgcta gatgactaaa tcagcttttg tgtaagttat ttctcagtaa tcaaggtgat
    43381 cagtattgga aaattgaaat ttagtaagga caggggacac aattacgcat gaaagtagaa
    43441 aaaattgttc ttatatccgt gttttttggg taggacagtc gcgtttgtga tttgtttcgt
    43501 gttataatac aactagttgt aaagaaagac acatccttta tataaaatgc gatgcaagag
    43561 gtgagacaaa atgagatgtc ccagatgtca acataataat tcacgagtaa tcgacagccg
    43621 tcaagcagat gatggacgtg cgatccgtcg ccgtcgcgaa tgtgagaatt gttcgttccg
    43681 attcactaca tttgaacgaa ttgaagctgc tccgttatta gtaatcaaga aaaacgggga
    43741 acgcgaagag ttcaatcgcg ataaaatttt aagaggactc atccgttcag cagaaaaaag
    43801 acctgtagct atggaacaaa tggaacaaat cgtcaaccat gtcgaaaacc gcgtacgcag
    43861 tctcggagaa aacgaagtat cgacgaatct aattggtgaa tatgtgatgg aagatttagt
    43921 tgatttagac gaaatcgctt atattcgttt tgcaagtgtt tatcgccagt tcaaagatat
    43981 gtctgttttc ttaaaagagt tgcaagatat cgtggataaa gcaaaagaaa ataataaaga
    44041 gaataataaa gaaaacaatt aagaaacaaa aaggaggcat ttccttggaa aatgcttgga
    44101 aagagcttca accaaaaaat atttaccaag gaagaaaagc ggctccgctg agagaagaca
    44161 gcaagaacgc cttgctgtat ctttatcagc cactaatcgg cggagaagcc ttgagtatct
    44221 atttcaccct gttatcagaa atctctcaag aatctggcaa aggtcctgaa gggatgcatg
    44281 cagatctgct tagtagtcta ggctgcggta tcccgcaatt ttatgatgca agaaaaaagc
    44341 tagaaggaat cggcttactg gatgtttatt ttaaagaaga tagtgatctg ggacctcgtt
    44401 ttatttatga gctgttagaa ccactcattc ctaaagcatt tttcaaagac tctgttctgg
    44461 cttttcttct tctggaaaaa gttggtgaac gacgattcca gaagcttacc caattctttg
    44521 aacctaaaag gcatacagca gagggatatc aaaaaatcac aaaaaaattt ttagatgttt
    44581 atcgcttcaa tgaagcagtg tacgctgcta atcaagaaaa catcgaatcg ctgaataaat
    44641 cttttgaaga atctgaatca gtagcattac ttgaagcaga ttcgacattc gattggcagt
    44701 ttttgaatga ttggctggca aaaaaacata ttgcaagtct tgatgatcca atcatcaaac
    44761 aagtcgacat gtatcatcag ctttacggtt tcgatgaatt gacacttggc gaactgatca
    44821 gtcaatctta tgactttagt gaacaaaaag tatctgaaaa agaactgcaa aaagccattt
    44881 tggcaatgca gagacgtccg gtgaagcaaa caaaagaaac agttgcggtt tctgaaaaca
    44941 gtaatgaacc ctcgtcaaac gaacatttga caaacgcagc gcttcagctg ataaaagaag
    45001 cacagagcat tccaccgatg aaatatctgg aagcaatcaa gtcagaaaaa ggcggatatg
    45061 tttctaaaca agaaaactgg ttactgcaag atcttgtttc tcaatcaggt ctttccagta
    45121 gcgtgatcaa tgttttgctg aattatgtac tagtgatcaa gaataatgct agcttaaatg
    45181 ccagttatgt aaatacagtg gcaaatgaat gggcacaaaa gaaaatcgcc acagcagaag
    45241 aagcgatcca gcacattcgg caaataagta atcaagcaaa acaatcaaaa caaaagaagc
    45301 aaacaaatta tcaaactggc aagcgaaatg ttcgacggga aaagcttcct gattgggtca
    45361 accagccaaa agatgaaaaa cagatcagca gcgagaaaaa agcagagatt gaccgccgtt
    45421 ttaaagaata cctggcaaaa aaggagggtg atatctagtg gaagacgtag gcaaagaatt
    45481 gaagaggatc atcagcaaac gcaattatca agaaaagtat gctgaaatga tcgctgaagt
    45541 cttaaatgat gcggatgttc aagcttttct aagcgcacac caagaagaat tgacacaagc
    45601 ggatattgaa aaaagttacg ctaaattgta tgaattcgtg caagaaaagc gtaaatatca
    45661 aaaaaacgat ccaacgatga tcgctcccgg ctatgagccg aagctgacgt tgaattttca
    45721 ttatgtggat gtcacttatg taccaacgga agcattgtta gcacggcaaa gacaagaaga
    45781 aatcagaagc cgagtaaaag caatggatat gccaaaagat attcaatcgg cttcttttga
    45841 aaactttgaa cgtacagatg gacgaggata tgcgagctta gaagccctag attttgtaga
    45901 gaaatatact gctgatccaa aagggtatca taaaggactg tatctagtag gaagttttgg
    45961 tattggaaaa acatatttat taggagcaac agcgagagaa ttagcgattg ctggattcac
    46021 cacgacgcta gtccattttc cgacatttgc cgttgagatg aaacaggcaa tcggaaaaga
    46081 tcaagtggca gaaaagttag atgcagtgaa acgttcacct attttgatga tcgatgacat
    46141 tggtgcagat gcgatgagta gttggatacg agacgaagta ttcggtgtta tccttcaata
    46201 tcggatgcag gaacagttac caaccttctt ctcatcgaat tttacaatgg atgaattgga
    46261 acagcatttg tccgtcactc aacgtggaga tgaagagccg ctaaaagcga aacggataat
    46321 ggaacgcata cgttatttga caaaagaaat cgaaatgact ggtcgcaacc gacgcaatgg
    46381 ttgaacagtt gacaagtcca actgacattt gacctagtag taaaagcagg tcagcgaatc
    46441 attatatgga cagaaatgac cggagacaat caccgcaaca agtaatgcag aaacttaaac
    46501 ggaggatttt ttatgagggc tattttttct agtatatctg tagttggtct attttttctt
    46561 ttagcaggct gcggtagcgc agatagcaac acaggttctg ttgattcgat tgatgcgggt
    46621 caaacagtaa aaacaagcag tacgaaaacg gagagacaaa gcatatttgt cggaacgatt
    46681 gaaagtatca ctcaagtaga agcagaagaa gaaacgcttc agttgaaact cgtcgatgtc
    46741 atggccaaag aagatccagc gaatatggga acgtcattca gcaatgacgg agtgatctta
    46801 aatgctactg ctgaaaaaat aagcgtggac ataaatgaat tgaaaatcgg tgacaaagtc
    46861 caatttacat tagttgagat gccgatcatg acgatgtcta tcccaccgca agttcctgga
    46921 gattcgatta ttgatgtttc attgaacaaa taaccaataa cattttttca aaaataagaa
    46981 aatgttgttt tatagaacga aaaggtatat tttttaagga gatatgggaa tttttttatt
    47041 cctagtctcc ttattttatt tggaaaatgc tataattatc tagaaggagt gaagctatga
    47101 accatttaga tttatctcag cgcttagccg cagtgggtga ctttgtccct gaatccgctc
    47161 gtttagcaga cattggatca gatcacgctt acttacccgt tgcattgata ttaagaggaa
    47221 aaatcgaata tgcagttgct ggtgaagtcg ttcaaggtcc ttatgaatca gcacgtaagc
    47281 aagtcaaaaa agatggatta gaagaccgta tcaccgtacg ccttgcaaat ggtctagatg
    47341 ctatccagcc acaagatggt attacagctg tcacgattgc gggtatggga ggttcactga
    47401 tccgagatat cctagaagct ggcaagttga atcaacgctt aagcggacaa gaacgtttga
    47461 tccttcagcc aaacatcgga gagcgtacat tacgatcttg gctgcaagaa aatcagtata
    47521 aaatccaatc agaaaaaata cttgaagaaa ataaaaaaat atatgaaatc attgtagccg
    47581 aaaaagaaca aaaaccagtt acttacaatg aaatggagct gatgttcggg ccatttctgc
    47641 ttgaagagaa aagtgagatc ttcaaatcaa aatggacaag agagcttcaa caaagaaaag
    47701 ctatccgtga acagctcaaa caagcagcta atcaagaggc acgtcaaaaa caaatcgagc
    47761 aggaaatcca aatgattgag gaggttttaa agccatgaca atcagcggac gcactttcat
    47821 ccaaaagttt gaaagctatt gtcctcagtg gctagctgaa gaaggagacc cagtcggact
    47881 gcatatcgga acattggata agccaatcca acgagtaatg atgacattag acgttcgtcc
    47941 agaagtggtc gaagaagcaa tcaagaaaaa aatcgatttg ctgattgcta aacatcctcc
    48001 aatctttcgt cctgtaaaac ggctggttac tgatcagcca caagaaaaaa tgtatgcaga
    48061 tttgttgaaa catgatattg ccgtctatgc agcacacacg aacatggata ttatttggga
    48121 tggactgaat gactggtttt gtgaacttct cggtattgaa gtggagagtt atttagtaaa
    48181 gactcatgag attcattata aaaaactggc cgtttatgta cctgtggatc atgcacagaa
    48241 aatgcgtgaa gtattagctg cagctggtgc tggtacacaa ggtgattata ccggtacaag
    48301 ttttacctcg attggacacg gtcgatttac tccggaagct ggtgcacaac cagcgattgg
    48361 gaaagtaggg aagacagaac aagttcaaga ggcaaaagtt gaggttattt tgccggaaac
    48421 tatcgaaaaa caagtgattc aagccatgcg gtcagctcat ccttatgagg aacctgctta
    48481 tgatcttttc gctatagatg aaccagtcga aatgtttggg ttaggacgag taggtgaatt
    48541 gccgcaagaa atttctatag aggcatttgt agaacaagta aaagaagcgt tccaactaga
    48601 tggactgaga atagtccagc caaaaaatgc aaaatcctct gtgaagcgca ttgcgatttg
    48661 cggtggaagc ggtgaaaaat tttatccgca agcaatcgcc caaagagcag atgtctatat
    48721 cacgggagat atctattatc atacggctca tgatatgcaa agcgctgggt tgattgcaat
    48781 tgatcccggt cattatattg aatcgttatg caagcaacga tttatcgaga aatttgaatc
    48841 ttggaaacaa gaagaaaatt gggatattaa ctttttcgtt tctgaaacaa atacaaaccc
    48901 atttcaattt aattaaaagg agagaatact atgtacgaaa atcttttgcc acgtttttta
    48961 cgatatgtca aaacagaaac acgctccgat gcaacaagca aaacgactcc atcgactcag
    49021 acacaagttg cttttgcgca agaattgaaa aaagaattag aagagttagg cttagctgat
    49081 gttttctata atgaagataa cggattcgtt atgggaacat taccaagcaa tgttgatcat
    49141 gaagtgcgtt ccattggatt tatttcccac atggatacag cagattttaa tgcagtagga
    49201 gtcaatccac aaatcattga aaactatgat ggcgagtcta cgatcgtact agatgcggaa
    49261 gaacgctata cattgaatac aaaagatttt ccaaatttga aaaattatga aggccacaca
    49321 ttgatcacaa cagatggcag tacattactg ggagcagatg acaaatcagg gattgcggaa
    49381 atcatgactg caatggagat cttgatcaat aatccttcta tccctcacgg taccatcaaa
    49441 gtggcttttg gtcctgatga agaaatcggt gtcggagcgg ataaattcga tgttgagcaa
    49501 ttcgatgtag attttgctta tacagtcgat ggtggtcctt taggtgagtt gcagtatgaa
    49561 acgtttagtg ctgctcaagc aaatattacg atccaaggga aaaatgtaca tccagggacg
    49621 gcaaaagata caatgatcaa tgctctacag ttagcaatcg attttcataa tcagcttcca
    49681 gccgatgaag ttccagaaaa aacggaagga tacgaaggtt tcttccattt gatggcattg
    49741 gatgggaatc ctgaagaagc ttctatgagt tatatcattc gcgatcatga tcgtgaaaaa
    49801 ttcgaagcac gcaaagcaca aatcacacat atccaagaag agttgaatca gcgatttgat
    49861 caagaacgaa tcaaagtaga tatgtatgat caatattaca acatgaaaga aatcattgaa
    49921 aaagatatga gcatcgtgga attagcgaaa caagcaatga tcgagcttgg tatctctcca
    49981 gtgatcgaac cagttcgtgg aggaacagat ggttcaaaaa tctcttacat gggtattccg
    50041 acgccaaatc tatttgccgg aggagaaaat atgcacggac gattcgaatt tgtttcttta
    50101 caagtaatgg aaaaagcaac agacgtgatt gtaaaaattg cagaattgaa cacaaagagt
    50161 taaaatcgaa aaagaacagc ttatgacaag gcgttcgtcc caatgatgga atgtcatctg
    50221 tctaaggcta aagggcagga caatggaatt ggttgtcccg ccctcttttt tgaagagtaa
    50281 ataggaggta aagaatggaa aagctgctag ttttttataa caaaagttca gggaaagatg
    50341 agggtgagaa attagcacgc tggtttcaag attatgtaaa agaaaagcag ccagaattat
    50401 atgtagaact agctgaaact ggcccatcga ttgatgatgc ggaattagtc aagaaagcag
    50461 aagccataga agctgatact ttagtaatta taggtggaga cggcacgatt catcatatcg
    50521 tccaagcgtt ccaaaaaaca ttatctcaat accatgtcgg attacttcca ggtggaactg
    50581 tcaacaacct tgcacgtgtt cttgagattc ccctcaaaaa agaagaagct gctgacgtga
    50641 tcctggaagg caatgtacgg aaaatagact atggtcaagt caatgatgaa gtcattatct
    50701 ctacactgac aatcggaatc ttagcagata cagcagtttg gatcagtcaa gaagaaaaac
    50761 agcgttacgg ctcgtggatc tttatcaaga agttttttaa gttattgctg aagaagaaaa
    50821 aatatcattt ggatttagaa acagacgagg agcattggaa aggaaaatcc caactgttga
    50881 cagtcacgat gtctaactcc gtgggcggtt ttacaaattt tgacgactca gcgaccctag
    50941 atgatggaaa atttcatgta acgatcgtac cttccttaga tattttccga tttatttttt
    51001 atttgcctag aatcatgaga ggcaaattct atacgatccc cggcattact tatttcacag
    51061 ccaaacgcat gaaaatccaa gcgcaggaaa aatctgtggg cactcgaacg gatggagaca
    51121 cgacagatga tcttccagtt gaattatctg ttgtatcaaa aggattaaca gtttattcac
    51181 caaagaaaga agaaaaagct tagaaatagt agttaacaga aagttggaag aagagtaatg
    51241 gatgtattgt tacacaatcc tttgtggttt taccttttca ttgtgaattt ttacttattt
    51301 tgtctgatgg ggtatgataa acatcaggct agaactagcg gctggcgtgt tcctgagaat
    51361 aatttgatat ttgtcagcct gttaggcggt ggtttgggag gattgatctc tcgaaaagtt
    51421 ttccatcata aaactcggaa aaagaagttt actataggat tcgctgcagg tgtacttgtt
    51481 gatttgattc tcatttattt tttccattaa ggagcaggag atgtcgtcta aaggaacgaa
    51541 aactaaaatc attctgaatc tgcttttgct gattgtgatt tttgccatta tttattattt
    51601 aatcaatcaa tcatttgcag atatctttgc cgaattgctg tctaccagtg ttgttgtttt
    51661 ggcagcggct gttatattag ggacagttta ccaaatcgct gaagggcgct caatcaaaga
    51721 aattgctgaa cctttcgcgc cgaattttac aacgaaagat gggttcttga cctctagcta
    51781 tattgcgttt taccgaatcg tttcgtttgg tacaggaacg ctgatctcgg agatttattt
    51841 ttataacaaa aaaggaatga agctatccca gggagcaggg gtgacagcac tccatatgat
    51901 tatgtacaaa ctggcagtga tcacttatgc attagctggc ttggtcatcc aattctctct
    51961 tttttattct aatggtccat caatgatctg gtttatcatc gctggtatcc ttttgacgat
    52021 cgtgattgtt gcctttttat tggcgatgtc cgtcagcttg aatgttcaaa tattttttgt
    52081 cagcttatcg aataagttat ttaaaagtga gcgtttgcgg aaatgggtgg atacatgcaa
    52141 tcagcagatc tactctttaa gggaaacggt ccaaaccatt atccaagaca ggactgcgct
    52201 attgcggatt tatctatgga atatgttaaa acttgtattt tggtatgtac ttccttattt
    52261 gattctgatt gaaaaccatc ctaaaataga ttttctactg accatttcgt ttatcagctt
    52321 tgcagtagtg ctttcagggg ttattcctac gcctgctggt attggttcct ttgaatttgt
    52381 ctatttgtta ttattcagac cagttgttgg aacagttgac gcagtttctt cgcttttact
    52441 gtatcgtttc agttccttta tcttgccatt tctctatgga tttgtgtatg tgattgctga
    52501 acggcgaaga gtgatcaaag aagaaataat agaagtaaaa aaagagaata aagaaaatca
    52561 agggtaggtc ttttataaga tctacccttt ttatcaaatc aaagaaaaac gttatggata
    52621 agataatttt acgtttgttt ggctcttttt ttatatacat acaatcaatt ttgttttttt
    52681 aagatttata taagtcgaga aaaaaagtca aaaaactatg tgtatatttt gaccaaaatt
    52741 gaccaaagcg atataatagt ttttgtaaag atcaaagaaa taagtgatct tacctttaat
    52801 taggagggta tacctgtatg aatattgaga aaatgactac gacgcttcaa gaagcgattg
    52861 ctgaagctca acagattgcg gtcactcgaa aacatcaaga tatcgacatt gcccatgtat
    52921 ggaaaatctt tttacaatct aatcattttg gtcgaaattt ctatacggat gccggattgg
    52981 atgtcgaatc ttttgaacat gaaattgatc gtctactgga tgagtatcca gttgtttctg
    53041 gtggaaacgt ccaatatgga caaaacctca gccagaattt ctttcgatta ttgaatgaag
    53101 cagaccaaat acgtgaaagc ttcggcgacg agtttctttc aacggaagtc gttattttag
    53161 cgttgatgaa attaaaaaat tatccgttaa ctgtttattt gaataaaaac ggattatctg
    53221 aaaaagaatt gcgtaaaaac atcgaagaga tgagaggagg agaaagagtg acctcacaaa
    53281 accaagaaga acaatacaaa gcattagaaa aatacggcgt tgatttagta cagcaagtca
    53341 aaagcggaaa aatggatcca atcatcggtc gtgatgaaga aatccgtgat gtcatccgta
    53401 ttctttcacg aaaaacaaaa aataatcctg ttctgatagg agaacccggt gtagggaaaa
    53461 cggcaatcgt tgaagggctt gctcaacgta tcgtacgcaa agatgtgcct gaaaatttaa
    53521 aagataaaac gattttttca ttagatatgg gtgcattgat cgctggagca aaattccgtg
    53581 gtgaatttga agaaagacta aaagcagtct tgaaagaagt caaaaaaagt gatggacgta
    53641 tcctcttgtt catcgatgaa atccacaata tcgtaggggc aggaaaaaca gaaggaagta
    53701 tggatgccgg aaatctgttg aaaccaatgc ttgctcgcgg tgaattacat ttgatcggtg
    53761 ccacaacttt agatgagtac cgtcaatata tggaaaaaga caaagcgttg gaacgtcgct
    53821 ttcaaaaggt attagtcaaa gaaccaacag tggaagatac gatcagtatt ctgcgtggat
    53881 taaaagaacg ttttgagatc caccatggtg ttaacatcca tgacaacgca ttagtagcag
    53941 cagctacttt atctgaccgt tatattactg atcggttctt accggacaaa gcaatcgatt
    54001 tgatcgatga agccagtgca acgattcggg tagaaatgaa ctctatgcca acggaattag
    54061 atcaagttac tagacgtttg atgcagttgg aaatcgaaga agctgcactg aaaaaagaat
    54121 cagatgatgc aagtaaaaaa cgcttgaaaa atttgcaaga agaattagct gaacttcgtg
    54181 aagaagcaaa tgcgatgaag atgcagtggg aaacagaaaa agaagaagtc aattctgttt
    54241 cagcaaaacg tgctgaaatc gataaagcaa aacacgaatt agaagatgca gaaaataact
    54301 atgatttaga acgcgctgct gtattgcgtc atggtacgat acctcaatta gaaaaagaat
    54361 tgaaggaatt agaagctaaa gcaaaagaca gcgagattaa gatggttcaa gagtccgtta
    54421 ccgaaaatga gattgctcaa gtcgttggca gattaacggg tatccccgta acgaaacttg
    54481 tagaaggtga acgagaaaaa ctaattaaat tgaatgaaac acttcataaa cgagtgattg
    54541 gtcaagatga agcagttgat gcggtcagtg acgcggtaat tcgttcacgg gctggattgc
    54601 aagacccaaa tcgtcctcta ggttctttcc ttttcctagg accaactggt gtcggaaaaa
    54661 ccgaattagc gaaagcgtta gctgagaact tgtttgactc agaagatcat atggtacgga
    54721 tcgatatgag tgaatatatg gaaaaacatg ctgtgtctcg tctagtcgga gctcctccag
    54781 gctatgtagg ttacgaagaa ggtggtcaat taacggaagc tgtcagacga aatccttata
    54841 caatcgtctt gttggatgaa atcgaaaaag cgcatcccga tgtcttcaat atcttgttac
    54901 aagtcctaga tgacggacgc ttgactgatt caaaagggcg agtagtagat ttcaaaaata
    54961 cggttctgat catgaccagc aatatcggtt cacagttact gctggaaggc gtgactgctg
    55021 atggaacgat tccagaagca gtggctgaac aagtcaacac gctattacgt ggaaacttca
    55081 aaccagaatt cttgaaccga atcgacgaca cgattttatt tacaccattg agcctagaca
    55141 acgtgaaagg aatcgttgac aaaatggtgg cgcaattggc acaacgtctg gaacatcaag
    55201 aaatcctgct gacaatcagt gatgaagcca aaacgtggat tgctgaaaat gcttatgaac
    55261 cagcatatgg tgcaagacca ttgaaacgat ttatcactaa ggaagtagaa acaccactag
    55321 ctaaagaaat cgttgcagga catgttatgc ctaaatcaaa agtaacgatt actttacttg
    55381 atggacaact tcatttcaaa acagaagagc tagaagaaat cgtctaatct tccattgaga
    55441 ctataaaaga aaaaaggggt ctgacacaac tagtgtcaga cctccttttt tcaaataaac
    55501 agtgttccag aagtagtttc ttcgaaaaat aagccgaaat ttcttgaagc tgcccgcttc
    55561 tgtcacaacc tcttttatta ggtatttttc actttttcta atttataact ttcaaagtaa
    55621 cgctcatctt ttttactttg agaccatgca aacaatccca ttaaggcagc acctatcgta
    55681 tttgtcagca agtcgcccat cgtatccatc aatgctgctc gtccgacaaa cagtgtgcca
    55741 tcagaagcag caaaacgttg aagattcatg cctaagaact ggtcgcataa gaattcccag
    55801 aactcccaaa acacgccgca aagtccagca aacgcaaagc cgaataagag gaacaaccaa
    55861 ggactggttt ttgatatctc agcatctttc atcaagtaac caatcaatcc atagccaaga
    55921 gctgtcagga ccattggact caccgcatgt aagattttgt cccaaaaaga gatgatactg
    55981 atcaaatgca tccctgtgcc taaaaagaca gagatgaaca agaaaaacca gtaaaacaaa
    56041 acgattgctg ctggtatttt aagtttgaaa actttcaaaa tgacttgtgg catgaaaatc
    56101 aatgcaatcc ctaataaaga ttccacgata aatgtgattc cttgagaagt cgaatatttt
    56161 tgtgtgataa attcataaaa attatagcag acaacgaata caccaaaagc aagtaaagct
    56221 atttgcacct ttttgatttc tttaatcact gaaagtccct ccatctaaaa atctttttac
    56281 ttctgaaagc aactcaattt ctgaagaaac cacttttccg ttcaatttga tcaaacctat
    56341 tgtatataag ttcaggtagt gaaattgatt ttcagctgtt tcttgtagtg cagcgatttt
    56401 tttcgggtgg gttccgcctt gctgtcgagt atcagtatat aagccgatca ctgggatgcc
    56461 tttcgcataa gccacaccga tttccgatgc taccccggca tcgatcgtca gcccatctag
    56521 taaagcaatc attaaatcac tagccaacac tttttctgtg tcagcaagag caatcattcg
    56581 actgtctgca taagcagttt tgtcattgat tgcttcattt tcttgaggaa gatagatcga
    56641 aagctcagaa gagagctctc tgatttcttt aactaactgt tggttatata acaaatcact
    56701 tttagcaaac attggtgcgg caaaataaat tttcatctaa acatcatcct ttttatcaaa
    56761 aataatcgct aaagtaagct tccatgtttg gaatgatacg ctctagtgac ttcagtgttt
    56821 cttcatctgt ttctaaccgt tcgattcttg ccaaataaga agcacggcga taattcatca
    56881 tcaagcaagt caatgtttgg atatccagtc gaacggattt tcctataggt tcatttaaga
    56941 cacggacttg tccctgttca tcccatgtta aagcgaagac accattattc caaggagcga
    57001 caggatcttc aatgatgaaa tgaaaagcat cagctgttcc aacaaacgga aaccgttgaa
    57061 ggaattcttt tacatcaacg atccgagcca taaaatatgg ttcgatctgc tctttgatct
    57121 gactatcttc aatcaaaaat gctaaaggct catttttata aatatctcca tgtacccagt
    57181 agatcatgga aaaatgtgcg gaaataaagt tccataagcc atttcgagct tcttgatcaa
    57241 gatagaacat ttcttttacg tgaaatattt cttcagctac ccagtagaac agaacacctc
    57301 ttggttcatg gttgctatcg tagtagacag cggctgtgcg ctcatcttca ttttcgaaac
    57361 gccaatactc ttcccaatga aaatcactgc ggaataaagc accatggttt tgttgggcaa
    57421 atttgtcata aacggaaaat acgtctggat gatcaactgg taatcgctca accataccac
    57481 ttactgggat ttgtttaggt agctgtgtat ctctgatttt aaaggaaagt ttatcagaca
    57541 tgatttccca tcctttacgc cgatagtagg ggatactgta aggatacaaa taagagatcc
    57601 attgcttatt cttgcgcata ttatccaatg caacgataat tagatcttgc atcaatccat
    57661 ggttagcata ttctggataa gttccaactc cagtcacacc acccatttta taacgtgcac
    57721 cgtgaatatt gacttcacaa ggatagatag cgatttgaga gatcagttta ttttcgtgga
    57781 accatccgaa cactttcgat acttccaaaa tcggtttttt ggaacggata aaagctcgtt
    57841 tattctcaaa cccgctttcc tcgatgtctg cttcagtcac ttggaacaca taggaaagca
    57901 attcattgaa ttgatcgata tgttcttgag tgaccggttt taattcaaga ttttctctaa
    57961 aatctttttg atccattttt tcatctccat ttattagctt gataacagta tagcaaaatt
    58021 ctacaaaaaa aacttctata gctgactaat taaaaactct ttaaattact gcttttcgtt
    58081 ggtataatgg aaagtgctga ttatatgaaa agaggagacg ctatggatat aaagaaaatg
    58141 aaagaacgcc aagagcagat tcgtaatttt tcgatcatcg ctcatatcga ccatgggaaa
    58201 tctacattgg ccgatcgtat tttagaaatg acgcacactg tgacgtctcg cgaaatgcaa
    58261 gatcagctgt tagactcgat ggatttggaa cgtgaacgtg gaatcacgat taaattaaat
    58321 gctgtagaac tcaattacac agcgaaaaat ggagaaacat atatctttca cttgattgac
    58381 accccagggc acgtcgactt cacctatgaa gtctctcgaa gccttgctgc ctgtgagggt
    58441 gccgttttag tggtcgatgc agcacaagga atcgaagcac aaacattggc caatgtttat
    58501 ttagcgttag acaatgattt ggagatcctg ccagtcatca ataaaatcga cctacccgca
    58561 gcagatcccg aacgtgtccg tcaagaaatc gaagacgtaa tcggaatcga tgctagtgaa
    58621 gctgtattag ccagtgccaa atcagggatt gggattgaag atattctcga gcagatcgtg
    58681 gaatatgttc ctgcaccaag tggagactta gatgcaccgc taaaagcttt gatctttgac
    58741 tctatttatg atagttaccg cggtgttgtt ctaaatgtcc ggatcacgga cggtatggtc
    58801 aaacccggag acaaaatcaa actgatgagt aatggcaaaa catttgatgt tacagaggtc
    58861 ggggtcttct cgccaaaagc agttgctaga gactttttga tggtcggtga tgtcggttat
    58921 atcacagcaa gtatcaaaac tgtccaagat acacgagtag gggatacagt aacgttagca
    58981 gacaatcctg ccgctgaagc tttaccaggt taccgaaaaa tgaatccgat ggtttattgt
    59041 ggtctttatc caattgatac ttctcgttac aacgatttgc gggaagcttt agaaaaactg
    59101 caattgaatg atgctgcttt acaattcgaa ccagaaacct ctcaagcatt aggttttggg
    59161 ttccgctgtg gattcctcgg acttcttcat atggatgtcg tccaagaacg cttggaacgg
    59221 gaattcaatc tagaattgat cacgactgca ccatccgtta tttaccatgt taataaaacg
    59281 gatggtacga ctgctacagt agataacccg gcagattttc ctgaacctgt aacgattcag
    59341 gatgtagaag aaccattcgt caaagcacag atcatggtcc caaatgattt tgtcggtgca
    59401 gtaatggaac tttctcaacg aaaacgggga gaattcatta cgatggatta tttagatgat
    59461 taccgagtaa atgtcgtcta taatattccg ctttctgaaa ttgtctttga ctttttcgac
    59521 aaattgaaat caagcacgaa aggctatgcc tcattggatt atgaaatgtc tggttatcaa
    59581 aaaagcaagt tagtgaagat ggacatcttg ctgaatggag aaaaagtcga tgcattaagc
    59641 tttatcgttc accgagactt tgcctatgaa cgtggaaaag caatcgttga aaaactgaaa
    59701 aaactaatcc cacgccaaca atttgaggtg ccaatccaag cagctattgg acaaaaaatc
    59761 gttgcccgtt cagacatcaa agccttgcgt aaaaacgtct tggctaaatg ttatggtgga
    59821 gatgtttctc gtaaacgtaa actcttagag aaacaaaaag aagggaagaa gcggatgaaa
    59881 caaatcggct ccgtcgaagt tccgcaagaa gccttcatgg ccgttctgaa aatggacgag
    59941 gacgaaccga agaaatagca agggtttgtt tgaaaacagt gtaaaaaata aaccatgttt
    60001 acatggattt gccaccggtt tgccaccggt ggtttttttc ggtggcaaaa atcaagaatt
    60061 ctttctaaga ttttccatga tatcgaccgt ttcatttctc atcttatctg ttacatgcga
    60121 gtaagtatcc atcgtaattg aaattctgct gtggcccaat cgttcagaga tttctttcat
    60181 ttttgcgcca ttttcgagaa gaagtgtagc atgagtatgt ctgagagaat ggaagttaaa
    60241 agagagggag agtgcattcg atatcctcct agtattccat ttcactacac ttggcgtaac
    60301 tagctcaccg tcttccttcg tacatactgc atttgaatca atgtagagct ttccatactt
    60361 cattcgattt tctaattgtt gtttcttatg ttttttcaga attgctagca aggtttgtcc
    60421 aataaaaatc gttcgattgg aactgcttgt ctttggcgta ccatataccc atgcgccgtc
    60481 attctttacc atttgtttct ctacagtaat tgttccattt gaaaaatcga catcatccca
    60541 cgtcagacca caaacttcgc caacgcgcat tcccgtataa aatccaatat tcaaaggaat
    60601 atagaaagga tgtccttcag gagtaatttc tagcatatga tcaaagtcct caagagaaat
    60661 gatttttaga tcttttttag tcgttggtcg tgcttcatat tttggtattt ttacatacag
    60721 cataggattt tgcttgatta atccccaagg ataaaccgcc atattcagcg cattcttaag
    60781 gacagagtga gtaatagtca ttgttttctt cgagtaaccc tttttaaatt cagcattgat
    60841 gaaattttgt aaaagagcag gggagagatc cgtaagtttt ttctttccta aataaccgtt
    60901 tatatgattt ttgatggtaa atcggtagtt ttcataggta ttgtatttta gatttagttt
    60961 aacgtattcc tccatccaaa aatcaaggta ttgttttact cgagtatccg tacctaaaaa
    61021 gtattgtcct gtttcgtcaa tatctgataa aacttttcgt aaagcagctt cggcctctgg
    61081 tcgggtgtct ccgccaactt tctccacttt ttttcttgag ccatcatcat tgatatct
//
LOCUS       DACKAC010000009        59453 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.009, whole genome shotgun sequence.
ACCESSION   DACKAC010000009 DACKAC010000000
VERSION     DACKAC010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.009"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            212..604
                     /locus_tag="I0B46_000569"
     CDS             212..604
                     /locus_tag="I0B46_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAR2082893.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="I0B46_000570"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="I0B46_000570"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAR2082894.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3165..4775
                     /locus_tag="I0B46_000571"
     CDS             3165..4775
                     /locus_tag="I0B46_000571"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAR2082895.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRSQKLIKAFVGASLADHETK"
     gene            5067..5936
                     /locus_tag="I0B46_000572"
     CDS             5067..5936
                     /locus_tag="I0B46_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAR2082896.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="I0B46_000573"
     CDS             6101..7372
                     /locus_tag="I0B46_000573"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAR2082897.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /locus_tag="I0B46_000574"
     CDS             7369..8664
                     /locus_tag="I0B46_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAR2082898.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="I0B46_000575"
     CDS             8763..9026
                     /locus_tag="I0B46_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAR2082899.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="I0B46_000576"
     CDS             9471..9920
                     /locus_tag="I0B46_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAR2082900.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="I0B46_000577"
     CDS             9977..11395
                     /locus_tag="I0B46_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAR2082901.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSISTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="I0B46_000578"
     CDS             11395..12255
                     /locus_tag="I0B46_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAR2082902.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="I0B46_000579"
     CDS             12429..13202
                     /locus_tag="I0B46_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAR2082903.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="I0B46_000580"
     CDS             13349..13714
                     /locus_tag="I0B46_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAR2082904.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="I0B46_000581"
     CDS             complement(13862..15070)
                     /locus_tag="I0B46_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAR2082905.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="I0B46_000582"
     CDS             complement(15089..15367)
                     /locus_tag="I0B46_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2082906.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15560..16216
                     /locus_tag="I0B46_000583"
     CDS             15560..16216
                     /locus_tag="I0B46_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAR2082907.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16658..17455)
                     /locus_tag="I0B46_000584"
     CDS             complement(16658..17455)
                     /locus_tag="I0B46_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAR2082908.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="I0B46_000585"
     CDS             complement(17452..18636)
                     /locus_tag="I0B46_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAR2082909.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="I0B46_000586"
     CDS             complement(18661..19908)
                     /locus_tag="I0B46_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAR2082910.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="I0B46_000587"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="I0B46_000587"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAR2082911.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="I0B46_000588"
     CDS             21888..22202
                     /locus_tag="I0B46_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAR2082912.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="I0B46_000589"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="I0B46_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAR2082913.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /locus_tag="I0B46_000590"
     CDS             22887..23531
                     /locus_tag="I0B46_000590"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAR2082914.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="I0B46_000591"
     CDS             23546..23875
                     /locus_tag="I0B46_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2082915.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="I0B46_000592"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="I0B46_000592"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAR2082916.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="I0B46_000593"
     CDS             24863..25687
                     /locus_tag="I0B46_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAR2082917.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="I0B46_000594"
     CDS             25680..26027
                     /locus_tag="I0B46_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAR2082918.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="I0B46_000595"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="I0B46_000595"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAR2082919.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="I0B46_000596"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="I0B46_000596"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAR2082920.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="I0B46_000597"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="I0B46_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAR2082921.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="I0B46_000598"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="I0B46_000598"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAR2082922.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31684..32451
                     /locus_tag="I0B46_000599"
     CDS             31684..32451
                     /locus_tag="I0B46_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAR2082923.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="I0B46_000600"
     CDS             32441..34483
                     /locus_tag="I0B46_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAR2082924.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34764..34913
                     /locus_tag="I0B46_000601"
     CDS             34764..34913
                     /locus_tag="I0B46_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAR2082925.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34924..36438
                     /gene="dltA"
                     /locus_tag="I0B46_000602"
     CDS             34924..36438
                     /gene="dltA"
                     /locus_tag="I0B46_000602"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAR2082926.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36435..37640
                     /gene="dltB"
                     /locus_tag="I0B46_000603"
     CDS             36435..37640
                     /gene="dltB"
                     /locus_tag="I0B46_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAR2082927.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37685..37918
                     /gene="dltC"
                     /locus_tag="I0B46_000604"
     CDS             37685..37918
                     /gene="dltC"
                     /locus_tag="I0B46_000604"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAR2082928.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            37921..39189
                     /gene="dltD"
                     /locus_tag="I0B46_000605"
     CDS             37921..39189
                     /gene="dltD"
                     /locus_tag="I0B46_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAR2082929.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39330..40412
                     /locus_tag="I0B46_000606"
     CDS             39330..40412
                     /locus_tag="I0B46_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAR2082930.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40412..41215
                     /locus_tag="I0B46_000607"
     CDS             40412..41215
                     /locus_tag="I0B46_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAR2082931.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGVNLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41285..42025
                     /locus_tag="I0B46_000608"
     CDS             41285..42025
                     /locus_tag="I0B46_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAR2082932.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42036..43040
                     /locus_tag="I0B46_000609"
     CDS             42036..43040
                     /locus_tag="I0B46_000609"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAR2082933.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(43085..44929)
                     /locus_tag="I0B46_000610"
     CDS             complement(43085..44929)
                     /locus_tag="I0B46_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2082934.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            45141..45659
                     /locus_tag="I0B46_000611"
     CDS             45141..45659
                     /locus_tag="I0B46_000611"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAR2082935.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            45787..46470
                     /locus_tag="I0B46_000612"
     CDS             45787..46470
                     /locus_tag="I0B46_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAR2082936.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            46779..46852
                     /locus_tag="I0B46_000613"
     tRNA            46779..46852
                     /locus_tag="I0B46_000613"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:46813..46815,aa:Arg,seq:tct)
     gene            47011..47370
                     /locus_tag="I0B46_000614"
     CDS             47011..47370
                     /locus_tag="I0B46_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAR2082937.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            47493..49124
                     /locus_tag="I0B46_000615"
     CDS             47493..49124
                     /locus_tag="I0B46_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAR2082938.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(49186..49827)
                     /locus_tag="I0B46_000616"
     CDS             complement(49186..49827)
                     /locus_tag="I0B46_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAR2082939.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            50109..50417
                     /gene="rplU"
                     /locus_tag="I0B46_000617"
     CDS             50109..50417
                     /gene="rplU"
                     /locus_tag="I0B46_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAR2082940.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            50444..50788
                     /locus_tag="I0B46_000618"
     CDS             50444..50788
                     /locus_tag="I0B46_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAR2082941.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            50801..51094
                     /gene="rpmA"
                     /locus_tag="I0B46_000619"
     CDS             50801..51094
                     /gene="rpmA"
                     /locus_tag="I0B46_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAR2082942.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(51187..52335)
                     /locus_tag="I0B46_000620"
     CDS             complement(51187..52335)
                     /locus_tag="I0B46_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAR2082943.1"
                     /translation="MAMIKQYKKKNGEKAWYFKTYLGIDPLTGKKKYTTKRGFRTQKE
                     AKTALSRLELELQKTGMPTSTNTTFKEAAELWLESYKKTVKESSYSRTKIIFNKHIYP
                     KFGNIKLSKINTAYCQKVVNDWSEKGTSKQYPLFINYMNKVFKYAINIGLTSDNPTLN
                     LLIPKPQIKTEKKLKLYTKEQLELFLNEVSQEQNPYFKNRDYTLFRLLAFSGCRIGEI
                     LALTWDNINFKTNEMAIKKTVARSDKYYISETPKTKKSNRIIYLDEKTIKQLKFWKLE
                     QRKYLFQLGFTKANYLFTNDENNFTINQSVAERYNIYRERAGLPYIGLHGFRHTHASM
                     LYEAGADHKEVQERMGHANIKTTMDTYTHITNSKKEETTQKLTNYINF"
     gene            complement(52347..52658)
                     /locus_tag="I0B46_000621"
     CDS             complement(52347..52658)
                     /locus_tag="I0B46_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2082944.1"
                     /translation="MIYTEFKEWLEKSTTGYETFIIKATNYQIEKNKNRPQKKRWDDK
                     KIDKAVLEMWKQVVTNLYQTIRKEKGVPLINGKEIWLEFIEEQGLIEFFNDSMAELEF
                     E"
     gene            complement(52711..53106)
                     /locus_tag="I0B46_000622"
     CDS             complement(52711..53106)
                     /locus_tag="I0B46_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAR2082945.1"
                     /translation="MIINELGVKVEERENLDADGHYVACMNTIVIKANLSKYRRQRTL
                     LHELGHASKHHDNYFLYNLAFSLHSKMEYEADRFMIEKLLDRYIAKSELEPHNINYMK
                     FIEDNNLSVRFEPLVKELLKARIYCYAAL"
     gene            complement(53142..53486)
                     /locus_tag="I0B46_000623"
     CDS             complement(53142..53486)
                     /locus_tag="I0B46_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAR2082946.1"
                     /translation="MLFERVKELCKKRGISISELENRIGFGKNSIYKWKTQSPKIETL
                     QKVADYFHVSTDYLLGRTDDPNAGVAPEERKLTVEEALASVMSSDGKPLTDNDREILS
                     AMIEAYLEKKDN"
     gene            53787..54008
                     /locus_tag="I0B46_000624"
     CDS             53787..54008
                     /locus_tag="I0B46_000624"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2082947.1"
                     /translation="MKQNKKTPSAKGALITLNFKDQKFSAVVDEIHPQEHQKNSFATD
                     EKKTQKRSEKVLRIIQFLLEKIRHFGSLF"
     gene            54135..54911
                     /locus_tag="I0B46_000625"
     CDS             54135..54911
                     /locus_tag="I0B46_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAR2082948.1"
                     /translation="MNTPQIFNFEQHEVRTVTIHDEPFFVGKDVAKVLGYQNGSRDIN
                     RHVDVEDRQNYQNGTFESPRGLTIINESGLYSLILGSKQPNAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATKLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMRQGYTKSHTTEIPK
                     SDGGTKVVMNTKWTQKGRLFIYESLKKEGYIPEIDLLEEE"
     gene            54908..55102
                     /locus_tag="I0B46_000626"
     CDS             54908..55102
                     /locus_tag="I0B46_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAR2082949.1"
                     /translation="MTMSQEFILKVRIQLAKYGKSQNWLADTIGISRPYMSDIMNGRR
                     KPDKQIKPIEAALAELEKEK"
     gene            55104..55388
                     /locus_tag="I0B46_000627"
     CDS             55104..55388
                     /locus_tag="I0B46_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2082950.1"
                     /translation="MQITIPDNLVVSELTTQITNAVLNSLDERLHLMNKSVELPPYPN
                     KSEVKKVLGIGDDKLTHWISLGLKTQQWSKLDIRIERSELQRFLKENFEF"
     gene            55409..55597
                     /locus_tag="I0B46_000628"
     CDS             55409..55597
                     /locus_tag="I0B46_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2082951.1"
                     /translation="MSYTLQQEHQILGLIKQRRKQLQDDRAALRKSDELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            55923..56162
                     /locus_tag="I0B46_000629"
     CDS             55923..56162
                     /locus_tag="I0B46_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2082952.1"
                     /translation="MENELSTLDQYLADPDWGKSNLQLVANKYEKSIDEAIVALTDAA
                     SITADDSKWTKRDLGILKRGVNWLEDQYREETKEK"
     gene            56168..56854
                     /locus_tag="I0B46_000630"
     CDS             56168..56854
                     /locus_tag="I0B46_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2082953.1"
                     /translation="MFKPLIDSYSAVLKKFKGNDIGATINEEVNIERLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGREISLK
                     DESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            56857..57687
                     /locus_tag="I0B46_000631"
     CDS             56857..57687
                     /locus_tag="I0B46_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAR2082954.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            57704..58555
                     /locus_tag="I0B46_000632"
     CDS             57704..58555
                     /locus_tag="I0B46_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAR2082955.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALEKAKSYTNEVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNTELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            58552..58911
                     /locus_tag="I0B46_000633"
     CDS             58552..58911
                     /locus_tag="I0B46_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAR2082956.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQA
                     PLILAKKYRNTFKEERF"
     gene            58926..59087
                     /locus_tag="I0B46_000634"
     CDS             58926..59087
                     /locus_tag="I0B46_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAR2082957.1"
                     /translation="MTTEEVIQMRIRNIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            59084..59389
                     /locus_tag="I0B46_000635"
     CDS             59084..59389
                     /locus_tag="I0B46_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2082958.1"
                     /translation="MSVDKKATMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
BASE COUNT        19337 a        10179 c        12220 g        17717 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgctcgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgattt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    34561 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    34621 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    34681 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    34741 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    34801 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    34861 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    34921 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    34981 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    35041 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    35101 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    35161 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    35221 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    35281 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    35341 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    35401 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    35461 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    35521 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    35581 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    35641 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    35701 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    35761 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    35821 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    35881 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    35941 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    36001 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    36061 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    36121 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    36181 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    36241 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    36301 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    36361 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    36421 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    36481 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    36541 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    36601 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    36661 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    36721 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    36781 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    36841 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    36901 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    36961 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    37021 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    37081 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    37141 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    37201 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    37261 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    37321 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    37381 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    37441 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    37501 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    37561 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    37621 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    37681 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    37741 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    37801 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    37861 agaggattgg agcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    37921 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    37981 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    38041 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    38101 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    38161 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    38221 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    38281 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    38341 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    38401 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    38461 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    38521 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    38581 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    38641 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    38701 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    38761 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    38821 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    38881 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    38941 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    39001 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    39061 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    39121 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    39181 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    39241 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    39301 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    39361 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    39421 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    39481 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    39541 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    39601 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    39661 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    39721 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    39781 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    39841 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    39901 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    39961 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    40021 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    40081 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    40141 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    40201 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    40261 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    40321 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    40381 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    40441 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    40501 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    40561 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    40621 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    40681 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcgtcaattt
    40741 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    40801 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    40861 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    40921 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    40981 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    41041 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    41101 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    41161 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    41221 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    41281 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    41341 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    41401 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    41461 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    41521 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    41581 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    41641 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    41701 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    41761 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    41821 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    41881 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    41941 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    42001 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    42061 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    42121 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    42181 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    42241 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    42301 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    42361 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    42421 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    42481 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    42541 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    42601 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    42661 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    42721 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    42781 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    42841 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    42901 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    42961 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    43021 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    43081 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    43141 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    43201 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    43261 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    43321 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    43381 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    43441 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    43501 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    43561 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    43621 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    43681 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    43741 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    43801 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    43861 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    43921 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    43981 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    44041 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    44101 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    44161 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    44221 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    44281 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    44341 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    44401 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
    44461 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    44521 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    44581 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    44641 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    44701 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    44761 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    44821 aataagcaat tcctgttaaa aataacaatg ataatataat gattatgctc cactttatac
    44881 gtaaataaaa agattctttt ttattatata tgttattaaa aaaagacata aaggtattcc
    44941 tttctaaaac ttgtaatgtt agggtaaacg ttttttaaac taacgtcaat tatttataat
    45001 aaaaaatgag ataaagatga ggaaatagaa ttagaaacat ataattatta aagggaaaca
    45061 tttttacagc tataatttga aaaaaattta tcgttattat atgattaaga aggattgaat
    45121 aaagaaagaa gttgaaaacg atacctgatt acaaaaaaat aacttttata agttctataa
    45181 cgttgattat atttttaatt cttattcttc tcgtcgtctt ggttccccga atttttggtt
    45241 tgactcctta tattattgag gacaaagaaa tgagtccata ttatgcacaa ggaaatctga
    45301 tatatgtacg accgaaaaat acaaatgaga ttttagtcgg agatattatt acatattatg
    45361 aaaattcagg aaaaaaaatc tcgacgcgtc gcgtaatagc cgttgaaaac aaccacgaag
    45421 atttttatac aaaagctgat acaaagactt atattgagcc tggagtagta aaaaagagaa
    45481 atattatcgg tagtcccatc tttcagattc catatattgg actagtcctt tcaaaaacgg
    45541 catttgcatg gattcagggg ttattcttta cgattgcttt ttgcctaaca ggaatcacag
    45601 catggaatac ctttgtagag ttgaaaaaga agcggagacg aacacagaaa tttttgtaag
    45661 aatgttaaaa acataaatgt attccagaga tccaacgact cttctgaaca tgaaacacga
    45721 gaaaacactc atgcagcttg tagatttagt cagttgttcg tataatgtgt gaagaggagg
    45781 agtagtatgc gcaatgaaat gatgcatcgt cctgtagtca aagaagaact attggatttt
    45841 atgcgtaccc agcaaaaaaa attaccgggt gagctaggca aactggaaga agaagctcat
    45901 gtagcagaag taccagttat tccccatgaa acagttgtat ttttgaaatt tttacttgga
    45961 cagcttcagc ctgaacgaat cttggagatc ggagcagcta tcggtttttc atcaagtgtc
    46021 atggccacga caatcggaga aaacgctcat gttacgacaa ttgatcgttt cgatgtcatg
    46081 atcgagaaag ctaaaaaaaa ctatgaacgt ctaggactga cagataaagt gactttgctt
    46141 gaaggtcaag cagcagatat cttgccggag ttgtctggtc cgtatgactt tatttttatg
    46201 gatagtgcaa aatcaaaata tattgaattt ttacctgaat gcttacgttt gttaagaaaa
    46261 ggtggcgttt tgatggtaga tgacatcttc caaggaggaa cgatcttgct cccagatgaa
    46321 gagattcccc gtggcaaacg agctatccat cgtaaattaa atgaatttct tcgtgttgtg
    46381 atggatcatc ccgatttgac atcgacaatc ttgccattag gtgatggtgt gatcttaatg
    46441 acaaaggaat cagaaacaat cgagttgtga tacatgatta tggcagcctg tagataatcg
    46501 attgaatcga gggctcaatt cgaacttgat gtaaaagttt ctatatttgt aagtcaatta
    46561 aaggatacag gaaatctaaa taaacaggag tagcatgaag tcaatgtagc tcctgttttc
    46621 aattgataac cttagaacag ctgaaggaga atgtctcgtt ttaaaagatt gatttagtag
    46681 ttgggccaaa ataattcatt atttgctaat taaataacta tttcgtattg acagaagcaa
    46741 tttaattatc tataattatt gatgttccgc tgattcttgt catagtagct cagctggata
    46801 gagcatccgc cttctaagcg gacggtcggg ggttcgaatc cctcctgtga cgctaaaaaa
    46861 ccgttagaaa ctgaggtttc taacggtttt tctttgtttt ttgtgccaat attgtttgtt
    46921 ttcaaaggca aataaaataa aaaaacatcc ttatcgagta caattataag gagcaaaaaa
    46981 ataaaactca tcagaaaaga ggaagaagat atgacgatac tatctagaat tttagtgact
    47041 ctagttgcaa ttgaattttt ttatatcatg tatatcgaaa cggtgagaac tgattctgac
    47101 acgacaagta gggtttttaa aatgagtaaa gaagagttaa gcagaaaatc tgttcaaaca
    47161 ttgtttaaga accaaggagt gtacaatgga ctattaggag tgggactact ttatggagct
    47221 tatctttctt ccgcatctaa agaaatcaca agtatgctat taatatctat tttttttgtt
    47281 gcattatatg gcagtttgac tagcgatcgt tggattttac tcaaacaagg tgggctagct
    47341 ctgttggcat tgctttctat tttcttataa atatagaaaa aataagaatt tatctaacag
    47401 gaaactttgt gaaaaatatt actttatctc atgaaatatg atattctaat gaatggtatt
    47461 ttagaaaatt tcatgaaaaa ggagttagat aggtgttcaa aaattatgtt tcctcactaa
    47521 aaaaagaatt ttcaggttat gatctttcaa gatttcagaa agatcttttg gctgggatca
    47581 cagtagctgc tgtagcattg cctttagcct tggcttttgg ggtatcaagc ggagctgatg
    47641 cggccgcagg tttgattacg gctatcattg ccggtatcgt tattggcggt ttatcaggcg
    47701 gtttttatca aatatcaggt ccgacaggtg cgatggcagc tatcttgatg tctattgcgg
    47761 caacaaaggg aatgcaaggt gtattgcttg ctactttttt agcgggtcta tttttacttg
    47821 cagcaggact tttccgatta ggaacactga cttcttttat cccagcaccc gtaattacgg
    47881 ggtttacttc aggtattgct atcattattg cattaggtca gatcgataat ttatttggtg
    47941 ttcactcgga aggtacaaac gttattgaaa aactaagtag ttatcaaacg ataggatttc
    48001 cgataaacac agcctctttg atcatgggag ggctagtcat tttagggatg tttatctatc
    48061 caaaaaaatg ggcgaaacga atgcctagtt cgctgctggc tatcattttg gcaacagcag
    48121 ttatgctatt gttccattta ccagtagcaa ctgtgggtaa gattccacaa acattgatca
    48181 gtagtaatcg tttgaatatg ggagatttta gcttttctgc cttgcaacaa gtggcagttc
    48241 cagctattag cattgcttta ttaggaatga ttgaaagctt gctatgtggt gcttcggctg
    48301 gccgaatggc aaataagccc ttagatagca atcaagaatt aatggcgcaa ggaattggaa
    48361 atctgttgct tcctttcttt ggcgggattc ctgctactgc ggccatcgcc cgtacaagcg
    48421 tggccatcaa atctggtgca caaactagat tagctggtat gatacatgca gttgtcttac
    48481 tgctctccat gcttgttttt gcgccagtga tgtcacagat tccaatgcca gcacttgcag
    48541 gtgttttgat tgtgacggct tggcgtatga atgaatggga aacgatcaag gagctattta
    48601 ctaataaata ttggtcagca gttctattgt ttatccttac gatgatttgt acagttatct
    48661 ttgatttaag tattgctatc gtgatcgggg tgatcggcgg ctgtgtattt tttgttgtca
    48721 aaagcgctgc tatcacgatt tcattagaaa cgatcgattg gcaaagaatg gggctgccag
    48781 aaacggaaaa gctgaataac tggacagtag tgtatattag tggtccactg ttttttatgt
    48841 cttcagaaaa actcaaaaat aaattggaag aactaaatca tcacgatggt gtcattttct
    48901 ccatgcgagg cgtaccaagt attgatttga cggcacgtgc agtttttgat gaatttcaag
    48961 aaaaagcaga gcaaagagga caaacgatta tctatacttc cttgcagcca gaagtagaaa
    49021 aacaactttc tcacttgtgg gaacatacat tcacgcatca acatcaaaca gttgctcatg
    49081 cgttgacagc tttacatgcg aaatatactg caattagcca ttaaaacaat gaaaaagaac
    49141 agacaagact gtaaactgaa gattttatgg tcttgtctgt tctttttaat attgctgtaa
    49201 aagtgaagct agtacttgcc agtcttgatg gataacaggt acaagggagc gattgtagaa
    49261 aatagctgct ggatgaaaaa gtggaagaat tcgatagttt ttttctgacc attcgtaggc
    49321 atccttttgt tctgttaatt gtaagacagg ccctgtatag agttgaccat gatttttggt
    49381 aaccgtatat tctttgccaa gtaaacgttg aagaccaatg tttccaagtg tcgcaattaa
    49441 ttttggctgg accttcatca gttcgtaatc caatattggt gcatgcgcaa atacttcgga
    49501 acgagtaggt gtccgatttg gataaacagt ttctgactgt tgtgtccgtt tattgatccg
    49561 atgagtcacg cgatacgggc ggctccttac agcactggtg atatacacct cttctcgtgt
    49621 taagccagtt gaagaaagtg cctgcatcaa ttcttttcca gcttgaccgc taaaaggaac
    49681 atgcgtatca atctctgttt tccctggggc ttctcccacg agcatcagtt taggatgaag
    49741 aggtccttgc ccagcgataa atccttctac aggacggccg ttcattctta aagaagcttc
    49801 ttgtatcagt tcttttgggt attccattta tgctcctcct ttttctttta ttataagaat
    49861 aaaagaaaca caatgcgact attttatttg gtagaactgt gaggatagaa aagcagaagt
    49921 attgcttgaa tttttgtttt actttttgat tttaaattta gttgactaac ggttgctgtt
    49981 ctggtaagct agtactgttg taattgtgga ctccacagct acaaccgctc agaacaagta
    50041 ataagcacag catatgctgt cacttcggat ggcgagtcta agtatatcag aaggaggtgc
    50101 tttatagcat gtacgcaatt attaaaactg gtggtaaaca agtaaaagtt gaagtgggac
    50161 aagcaatcta tgttgaaaaa cttgacgttg aagcaggaga aaaagttgtt tttgacgaag
    50221 ttatcttagt aggcggagaa tctacaaaag taggcgctcc aactgttgca ggtgcaactg
    50281 ttgaaggtac tgttgaaaaa cacggcaaac aaaagaaagt cgttactttc aaatacaaac
    50341 ctaaaaaaca ttcacaccgt aaacaaggtc atcgtcaacc atatacaaaa gtggttatcg
    50401 acgcgatcaa cgcttaatag cgtttgagaa agaaggccta tatatgatca aaggaacatt
    50461 taagcgaaat gacgcaggtc agatcgtttc attcactttg accgggcatg ctgatgctgg
    50521 tccttatgga agtgatatcg tttgtgcggg tgtttctgcg ttagcgatca gcactgtcaa
    50581 cgggattgcc tcattagccg gttttgagcc gattgttgaa atgaatgaag aagaaggcgg
    50641 ttatctttat accgaagtca cttcaggaat gacacaagaa caaaacaata ttgctgagat
    50701 tctattagaa aatcttttat taggattaca atcgatagaa gctgaaaact cagaatatat
    50761 ccaaattaaa acaattaatg ataaatagga ggtgcagacc atgttgttat ctatgaatct
    50821 acaattattt gcccacaaaa aaggtggcgg ttctacatct aacggccgtg actcagaatc
    50881 aaaacgtcta ggtgctaaac gtgctgatgg acaaacagtt acaggtggat caattcttta
    50941 ccgtcaacgc ggtacaaaaa tctatccagg tgtaaacgtg ggtatcggtg gagacgacac
    51001 tttatttgca aaagttgatg gcgtagtacg tttcgaacgt aaaggccgta acaaaaaaca
    51061 agtgtctgtt tatccagtag ctcaagaagc ttaataaata aactgctcta tctcttgtgt
    51121 agcaagggat ggagcttttt tgtaaattat gggatttttt ttgaccatac ttttgaccat
    51181 actttcttag aagttaatat aatttgttag tttttgtgtt gtttcttctt ttttgctgtt
    51241 agtaatgtgt gtatatgtgt ccatagtagt ttttatattt gcgtggccca ttctttcttg
    51301 gacttctttg tgatctgcgc ctgcctcata tagcattgat gcatgtgtat gtctaaaacc
    51361 atgcagaccg atataaggta agccggcacg ctcacgatat atattgtatc tttctgccac
    51421 tgactgatta attgtgaaat tattttcgtc attggtaaac aaataattag ctttagtaaa
    51481 tcctaattga aataagtact ttctttgttc gagcttccaa aattttagtt gctttatagt
    51541 tttttcatct aaataaatta ttcgatttga ttttttggtt ttaggagttt cagatatata
    51601 atatttatct gaacgagcta cagttttttt aatggccatt tcatttgttt taaaattaat
    51661 attgtcccaa gtgagtgcta atatttcgcc gattctacat ccgctgaatg ctaatagtct
    51721 aaagagcgta tagtctctgt ttttaaaata tggattctgt tcttgagata cttcatttag
    51781 aaataattcc aactgttctt ttgtatataa ttttaatttc ttttctgttt taatttgtgg
    51841 ctttggaata agtaaattta atgttggatt atcagatgtt aaaccaatat ttatagcata
    51901 cttaaaaact ttattcatat agtttatgaa aagagggtac tgctttgaag ttcctttttc
    51961 actccaatca tttactacct tttgacaata tgccgtatta attttagaaa gcttaatatt
    52021 tccaaatttg ggatatatat gtttattaaa gattatttta gtccttgaat atgaactttc
    52081 ttttacagtt tttttgtagc tttctagcca taattctgct gcttctttga aagtagtatt
    52141 tgtacttgtg ggcattcctg ttttttgtaa ttctagttct aacctagaaa gtgctgtttt
    52201 tgcttctttt tgtgttctaa atcctcgttt agtagtatat tttttctttc cagttagcgg
    52261 atcaataccg agataagttt taaagtacca tgctttttcg ccatttttct ttttatattg
    52321 ttttatcatt gccatcaata ttacccctat tcaaattcta attctgccat gctatcattg
    52381 aaaaattcga tcagtccttg ttcctctata aattcaagcc atatttcctt cccgttaatt
    52441 aatggaactc ctttttcttt acgaattgtt tgatacaagt tagtcacgac ttgtttccac
    52501 atttctaata cagctttatc tattttctta tcatcccagc gttttttttg gggtctattt
    52561 ttgttttttt caatttgata attagtagct ttgatgataa atgtttcgta tccggttgtg
    52621 cttttttcta accattcttt gaattctgta tatatcattt ttgttcacct ttctattttt
    52681 gaacgtatgt tcttttttgc ttaaaaaatt ttagagagct gcataacaat agatgcgagc
    52741 ttttaataat tctttcacaa gtggttcgaa gcgaacgctt aaattgttat cttctataaa
    52801 tttcatgtaa ttgatattgt gtggttctaa ttcagacttt gcaatatatc tatctaataa
    52861 tttttcaatc atgaagcgat cagcctcata ttccattttt gaatggagag agaatgctaa
    52921 gttatataaa aaataattat catgatgttt agaagcgtgt cctaattcat gtaataaggt
    52981 tctttgtctt ctatacttag ataaattagc ttttattact atggtattca tacaagcaac
    53041 ataatggcca tcggcatcaa ggttttcacg ctcttctacc ttaacgccga gttcattaat
    53101 tatcatttca atttgactgt ccaaatgact cacctactta tttagttatc ttttttctct
    53161 aaatatgctt caatcatagc tgatagtatt tccctatcat tatcggtgag cggttttccg
    53221 tcgctactca taacagatgc taaagcttct tccacggtta gttttctttc ctctggtgca
    53281 actcccgcgt taggatcatc tgtgcgtcct agcaaataat ctgttgatac atggaagtag
    53341 tcagcaactt tttgcaaagt ttctatttta ggactttgtg tcttccattt ataaatagag
    53401 ttttttccaa atcctattct attttctaat tcagaaatag aaatacctct ttttttacag
    53461 agctctttaa ccctttcgaa aagcatgtca aatcaacctt cctagagcgt acgataataa
    53521 tttatacaaa aagatagaaa ggtagttgac aaatctatcc aattgtctta tatttaactc
    53581 gtaagctaat ttaataagct aacaagcaac aagaaataca cctaataaaa taaacgaatt
    53641 cgaggtcggc aaactaagaa tgttaatttt tagggttttt ctaggcttat ttaactgtat
    53701 ataaatataa gacagttgga tagaaatgtc aataaaaatt agctaatttt attagcttac
    53761 atatttaaag aaaaggagga aacatcatga aacaaaataa aaaaactcct tccgcaaaag
    53821 gagctttaat aactttaaat tttaaagacc aaaagtttag tgctgttgtt gatgaaattc
    53881 atccacaaga gcatcaaaaa aattcttttg caactgacga gaaaaagact cagaaacgct
    53941 ctgagaaagt tcttcgaata attcaatttc tgttggaaaa gattcgtcat tttggaagtc
    54001 tattttgaat tctttattaa cagtcttgaa tacggtttgt ttgaataagg cgtcgacatc
    54061 catattaaat tcttccatac tattttcacc tcgctttcta ttcaaaatta tatcaaagaa
    54121 aggaatgagc aaaaatgaac acaccgcaaa tttttaactt tgaacaacat gaagtaagaa
    54181 cagtaacaat acatgatgaa ccattttttg ttggaaaaga tgtggctaaa gttctagggt
    54241 accaaaatgg tagtagagat attaacagac acgtcgatgt agaagatcgg caaaactacc
    54301 aaaacggtac ttttgaatcg cctagagggt taactattat taacgaatca ggtttgtact
    54361 ccttaatttt aggaagcaaa caaccaaatg ctaagaaatt taaacgttgg gtgactagcg
    54421 aagtgttgcc agcaattaga aaacatggag gttatctaac tccagaaaaa gtagaagaag
    54481 ctttgcttaa tccagataca atcatccaat tagcaactaa gttgaaagaa gaacgtactg
    54541 gaagactaat cgcagaacaa aaaattgcag aatacgaacc caaaatttcg tatttggata
    54601 gtatcttatc ttctacagat tcagtaacta ttagtcaaat cgcggctgat tatgggatgt
    54661 ctccacaaca gatgaacaaa ctacttcata agctaggtat tcagaaaaaa gtaggcaacc
    54721 aatggttatt atgcaaaaaa cacatgagac agggttacac aaaatctcat acaactgaga
    54781 ttccgaaatc tgatggcgga acaaaagttg tgatgaatac aaaatggact caaaaagggc
    54841 gtctgttcat ttatgagtcg ctgaaaaaag aaggatatat tccagaaatc gatttattag
    54901 aggaggaatg acgatgtcac aagaatttat tttgaaagtc agaattcaat tagcaaaata
    54961 cggaaagtct cagaactggt tagctgatac tatcggaatt tctagaccat atatgtcgga
    55021 cattatgaat gggcgtagaa aaccagataa acaaatcaag ccaattgagg cagcactagc
    55081 agagttagag aaggagaaat aacatgcaaa taacaattcc agataattta gtagtttccg
    55141 aattaactac acagattacg aatgctgttc ttaattcatt ggacgaacga ttacacctta
    55201 tgaacaaatc agtagagctt cctccatatc caaacaaatc agaggtaaaa aaagttttag
    55261 gcattggtga tgacaaatta acacattgga taagcctagg cttaaaaaca cagcagtgga
    55321 gcaagttaga catcagaatt gaacgatcgg aactccaaag atttttgaaa gaaaactttg
    55381 agttctaaag gcaaaggaga atgattttat gtcctacaca ttgcaacaag aacatcaaat
    55441 tctaggtctg attaaacagc gtagaaaaca attacaagat gatcgtgcag cgcttagaaa
    55501 atccgatgag ctatcagata gacaagctga actaattgct tctgaacttg aggatttgag
    55561 aatgctagaa ataaaaaata gggagattag attatgaaga agacagacac actttttata
    55621 ggattcattt tggggttatt agtgattgta gcgcaccaaa gtattatcgg aggaagcttg
    55681 ttcgcagcat tgatggtttt aatcaatctg cttgactcaa aagaaaggag caactatggc
    55741 acgagaagaa gcgctaaaaa tcggtaaagt gattgctgat aattggtggg caaatagccg
    55801 ttctattatt ttaagcaagc aacatatcga aaagaaaaaa gcatggcaac aaataaaaaa
    55861 gtgactccgc cgaccaaagc aatgagtcac tcaaaaaatc ataactaagg agattttaac
    55921 atatggaaaa tgaactttcc actctagatc aatatttggc cgatcctgat tggggcaaat
    55981 cgaatttaca actggttgct aacaagtacg aaaaatcaat tgatgaagca attgttgcat
    56041 tgactgatgc agcttccata accgctgatg acagtaaatg gaccaagaga gacttgggca
    56101 ttctaaaacg aggcgttaat tggcttgaag atcagtacag agaagaaaca aaagagaagt
    56161 gatatgagtg tttaaaccac taatagattc atactcggcg gtactaaaga aatttaaagg
    56221 taacgacatt ggtgcaacaa tcaacgaaga agtaaatatc gaacggctga aaacgatgta
    56281 tgacggatat gatggcgatc gagtcattga aattcgcttt attgatccac gacggttcac
    56341 agtacagcaa agaaacttca tctatgcgct cataggcgat attttcatcg atacaggcat
    56401 gccaacggac ttctggaagg aattcttcta cttccgtttt gaaggtgtca cagggcgcga
    56461 aataagcctc aaagacgaat cgaatacaac cgtgagtgat gccaatatct tagcgaatat
    56521 catcctagat ttcatctttg aacatcatat tcctttcaaa gaaggatatg agattttacc
    56581 agcgaatcaa gagtattact tctacaaatg cattacaaaa agagtctgct gcatctgtgg
    56641 caaaacagga gctgatatcg atcactttga caaagcgctg ggaagacgaa agcgcaaaga
    56701 agttgatcat tcagagtaca catttgcagc actctgcaga atccatcaca cagagaaaca
    56761 caaaataggt gtgatcaatt tcaaaaataa atatcaaatc aaagggatca agttaaacca
    56821 ggaaacaatt aaaaagttaa ggataggagg ataaatttgg ctgagataag ttggatcaaa
    56881 cttaaaacta ctatgtttga cgatgaaaaa atacgattaa tccaagctgt tcctgagtcg
    56941 gatgccatca tcgttatatg gattcgatta ctagttttag caggaaagac taacgacgat
    57001 ggtctgatat atatccagag gaacatgcct tataccgaag aaatgcttgc tacattgttt
    57061 ggcaaaaacg taaatacggt tcgcttagcg ctaactacat tggcaaattt caacatgatt
    57121 gatctaagca gtgatggact aattgccatc agtaattggg aaaaacatca aaatatcgag
    57181 ggtatggata aagtaaggct aaaaaatgct gaaagaaacc gtaaatacag ggaaagaaag
    57241 agacaggaac gtctcaaatt ggaaaatgac gttagcgtga cgtcacgtga cggtacagat
    57301 aaagatatag aagaagataa agatatagat aaagaagaaa agaaaggtaa gtattctgac
    57361 gaacacttac gccttgctaa aaagttgcaa agtaatttaa ctgaagattt tccaaaagaa
    57421 atgaacaaag tagatatcga aaaatgggca gacacaatca ggttgatgga agaaagagat
    57481 aaagcatcta tagaagcgat tgagtatgtg atcaattggc tacctacaaa tgaattttgg
    57541 tttggaaata ttagaagtgc taagaaattg agagaaaaat ttgagaagct caaattcgaa
    57601 atcaaagcag acaagaagaa tcataaaaag caaagtcaaa aactacagta cagcaatcct
    57661 agtgaatatg acgacttgcc aatttaaaaa ggagatgcat cacatggaaa gcctagcaaa
    57721 tgctatggag aaactaataa gaagagtatt agtgcaaagc ggaaaatgtc cagaatgtag
    57781 cgaacctttg tatagttggc gagctaaaaa taaggatggt tcagaacgtt gtaaaccaac
    57841 atgcatgagt tgtggttata aagcgttacg tgtgaaagag gatatacaga ccgaacggat
    57901 atataacgat agcttaaaag cacgagcgtt gagttttttt caaaatggtt cggtattaac
    57961 agataaaact ttgtttaaat gcaaaatgga aaattatcac gtagtggatc aagaaacaaa
    58021 aatcgcttta gaaaaagcaa aaagctatac gaatgaggtt ctgctgaacc atcctgcaca
    58081 tttcattcta tcagggaaat caggaagcgg aaaaagccac ttatcaatgg ccacagcttg
    58141 ggaaatactt gagcgctcaa attatgacaa gaaaatactt tttataagct atcaagaatt
    58201 attagagcaa ataaagtttt cttataacaa tactgaactg agaaaagaaa ttgaaggatc
    58261 gcttatagcc gatattaaaa caactgattt ggtggttttt gacgatattg gagctgaatt
    58321 aggtagtggg gtatcaaata gtaggcagtt tacaaacaac acgttaaaca cgctcttaga
    58381 agccagacag aacaaggcaa cgatcatcac aacaaactta tctggtcctg aactaagaga
    58441 agcctacggt gaaagaattg tttctaggat atttaagaat tcagaaggtt atgcgctgaa
    58501 attccaacaa acagcagaca agcgcataaa accagtgaaa ggtagtatcg catgaataaa
    58561 taccgtaata aaaaaactgt tcatcgaggt atcaagtttg attctatcgc agaagcagag
    58621 tattacgatc tagccttgtg gcaagctgaa gcaaatggct ggaaagtaaa acttcaggaa
    58681 agatttgagc tgatgccgaa atttgaacta gacggaaaga agtatcgcaa gatcgagtat
    58741 attcccgact tcacatttta taaaaacggc aaacttgtca aagtcgtaga tgtcaaagga
    58801 atgcagacaa aagactttaa gatcaaggca aagttgttct gtcatcaata tcaagcgccg
    58861 ttgatattag ctaaaaaata tcggaatacg ttcaaggaag agcgttttta acgaggtggt
    58921 ccatcatgac aacagaagaa gtgattcaaa tgcgtattcg aaacattcag cgtgaaattg
    58981 acgatctaga acgaacaaag gcagtgatgg tcaatgaaac ggcgagaaag gcaatcgatt
    59041 tgcacataga gaacttaaga agggaaattc gtagattgga ggaatgagcg tggataagaa
    59101 agcgacaatg aaacgaattg ctgaattaac caagtcagaa tcttggcagg aagacaaaga
    59161 aatagttgca gaagtccaaa agctcggtaa atcaatgtgg actgaaaagc ctaaacggaa
    59221 aacgccgaga aaaattgcaa tctggcatgg tgatcgaatt ctagtaacag gtacagctga
    59281 acagttatct gaaattactg gattaagcaa aaacattatc tgggatagag ctaggagctt
    59341 atggattgat tcaaaaggac gacagtttag gtatgtggag gagagataat ggatctcatt
    59401 acacaataca gtgacatcat cctcaagaaa atcatgatga agattcagaa aga
//
LOCUS       DACKAC010000010        59376 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.010, whole genome shotgun sequence.
ACCESSION   DACKAC010000010 DACKAC010000000
VERSION     DACKAC010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.010"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(159..1469)
                     /locus_tag="I0B46_000636"
     CDS             complement(159..1469)
                     /locus_tag="I0B46_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAR2082959.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1481..2899)
                     /locus_tag="I0B46_000637"
     CDS             complement(1481..2899)
                     /locus_tag="I0B46_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAR2082960.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3139..4707)
                     /locus_tag="I0B46_000638"
     CDS             complement(3139..4707)
                     /locus_tag="I0B46_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAR2082961.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4711..5121)
                     /locus_tag="I0B46_000639"
     CDS             complement(4711..5121)
                     /locus_tag="I0B46_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAR2082962.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5118..6041)
                     /locus_tag="I0B46_000640"
     CDS             complement(5118..6041)
                     /locus_tag="I0B46_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAR2082963.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6038..6916)
                     /locus_tag="I0B46_000641"
     CDS             complement(6038..6916)
                     /locus_tag="I0B46_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAR2082964.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6903..8240)
                     /locus_tag="I0B46_000642"
     CDS             complement(6903..8240)
                     /locus_tag="I0B46_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAR2082965.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8243..9346)
                     /locus_tag="I0B46_000643"
     CDS             complement(8243..9346)
                     /locus_tag="I0B46_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAR2082966.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9420..10928)
                     /locus_tag="I0B46_000644"
     CDS             complement(9420..10928)
                     /locus_tag="I0B46_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAR2082967.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10894..11586)
                     /locus_tag="I0B46_000645"
     CDS             complement(10894..11586)
                     /locus_tag="I0B46_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAR2082968.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11608..12582)
                     /locus_tag="I0B46_000646"
     CDS             complement(11608..12582)
                     /locus_tag="I0B46_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAR2082969.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12882..13754
                     /locus_tag="I0B46_000647"
     CDS             12882..13754
                     /locus_tag="I0B46_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAR2082970.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14212..16410)
                     /locus_tag="I0B46_000648"
     CDS             complement(14212..16410)
                     /locus_tag="I0B46_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAR2082971.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16642..17634
                     /locus_tag="I0B46_000649"
     CDS             16642..17634
                     /locus_tag="I0B46_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAR2082972.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17694..19115
                     /locus_tag="I0B46_000650"
     CDS             17694..19115
                     /locus_tag="I0B46_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAR2082973.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19108..20607
                     /locus_tag="I0B46_000651"
     CDS             19108..20607
                     /locus_tag="I0B46_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAR2082974.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20646..22040
                     /gene="uxaC"
                     /locus_tag="I0B46_000652"
     CDS             20646..22040
                     /gene="uxaC"
                     /locus_tag="I0B46_000652"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAR2082975.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22199..22882
                     /locus_tag="I0B46_000653"
     CDS             22199..22882
                     /locus_tag="I0B46_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAR2082976.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            22884..23720
                     /locus_tag="I0B46_000654"
     CDS             22884..23720
                     /locus_tag="I0B46_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAR2082977.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            23731..25485
                     /locus_tag="I0B46_000655"
     CDS             23731..25485
                     /locus_tag="I0B46_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAR2082978.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(25761..26102)
                     /locus_tag="I0B46_000656"
     CDS             complement(25761..26102)
                     /locus_tag="I0B46_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAR2082979.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26170..28341)
                     /locus_tag="I0B46_000657"
     CDS             complement(26170..28341)
                     /locus_tag="I0B46_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAR2082980.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28552..29409
                     /locus_tag="I0B46_000658"
     CDS             28552..29409
                     /locus_tag="I0B46_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAR2082981.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(29480..30337)
                     /locus_tag="I0B46_000659"
     CDS             complement(29480..30337)
                     /locus_tag="I0B46_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAR2082982.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30322..33024)
                     /locus_tag="I0B46_000660"
     CDS             complement(30322..33024)
                     /locus_tag="I0B46_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAR2082983.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(33037..34326)
                     /locus_tag="I0B46_000661"
     CDS             complement(33037..34326)
                     /locus_tag="I0B46_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAR2082984.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            34476..35522
                     /locus_tag="I0B46_000662"
     CDS             34476..35522
                     /locus_tag="I0B46_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAR2082985.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            35524..37677
                     /locus_tag="I0B46_000663"
     CDS             35524..37677
                     /locus_tag="I0B46_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAR2082986.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(38150..39601)
                     /locus_tag="I0B46_000664"
     CDS             complement(38150..39601)
                     /locus_tag="I0B46_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAR2082987.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(39598..41322)
                     /locus_tag="I0B46_000665"
     CDS             complement(39598..41322)
                     /locus_tag="I0B46_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAR2082988.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            complement(41315..41929)
                     /locus_tag="I0B46_000666"
     CDS             complement(41315..41929)
                     /locus_tag="I0B46_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAR2082989.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            complement(42274..43731)
                     /locus_tag="I0B46_000667"
     CDS             complement(42274..43731)
                     /locus_tag="I0B46_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAR2082990.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            complement(43772..44692)
                     /locus_tag="I0B46_000668"
     CDS             complement(43772..44692)
                     /locus_tag="I0B46_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAR2082991.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            complement(44704..45651)
                     /locus_tag="I0B46_000669"
     CDS             complement(44704..45651)
                     /locus_tag="I0B46_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAR2082992.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            complement(46130..46849)
                     /locus_tag="I0B46_000670"
     CDS             complement(46130..46849)
                     /locus_tag="I0B46_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAR2082993.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(46898..48544)
                     /locus_tag="I0B46_000671"
     CDS             complement(46898..48544)
                     /locus_tag="I0B46_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAR2082994.1"
                     /translation="MDNQRKPSLDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            48723..49313
                     /locus_tag="I0B46_000672"
     CDS             48723..49313
                     /locus_tag="I0B46_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAR2082995.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            49405..50910
                     /locus_tag="I0B46_000673"
     CDS             49405..50910
                     /locus_tag="I0B46_000673"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAR2082996.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(50994..52346)
                     /gene="celB"
                     /locus_tag="I0B46_000674"
     CDS             complement(50994..52346)
                     /gene="celB"
                     /locus_tag="I0B46_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAR2082997.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(52346..52864)
                     /locus_tag="I0B46_000675"
     CDS             complement(52346..52864)
                     /locus_tag="I0B46_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2082998.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(52880..53206)
                     /locus_tag="I0B46_000676"
     CDS             complement(52880..53206)
                     /locus_tag="I0B46_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAR2082999.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(53219..55198)
                     /locus_tag="I0B46_000677"
     CDS             complement(53219..55198)
                     /locus_tag="I0B46_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAR2083000.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(55219..55533)
                     /locus_tag="I0B46_000678"
     CDS             complement(55219..55533)
                     /locus_tag="I0B46_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAR2083001.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            55701..55994
                     /locus_tag="I0B46_000679"
     CDS             55701..55994
                     /locus_tag="I0B46_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAR2083002.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(56072..57184)
                     /locus_tag="I0B46_000680"
     CDS             complement(56072..57184)
                     /locus_tag="I0B46_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAR2083003.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            57571..57750
                     /locus_tag="I0B46_000681"
     CDS             57571..57750
                     /locus_tag="I0B46_000681"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083004.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLIEKVSLFFISKSC"
     gene            57944..58114
                     /locus_tag="I0B46_000682"
     CDS             57944..58114
                     /locus_tag="I0B46_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAR2083005.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(58538..>59376)
                     /locus_tag="I0B46_000683"
     CDS             complement(58538..>59376)
                     /locus_tag="I0B46_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAR2083006.1"
                     /translation="YQLVETQAPTGYELDATPIEFEIERSQTAVVELTKANRLTPGGV
                     VLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAP
                     TGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDA
                     NGTVLQSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIF
                     LTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKR
                     NI"
BASE COUNT        17760 a        12143 c        10390 g        19083 t
ORIGIN      
        1 acgacttagt tatcttatca cgtctaaaaa catgtgtcaa ctcttttttg gaattttcat
       61 aaaatgtttc aatgaagcgc gacaacgttg tttaatatat cattaaaaaa aataaaggtc
      121 aagctaaaat attatttaag cttgacctta ttataatttc aacgattgtc aaaagtattg
      181 tttgcagctg tgttgccagt ttctatagat aaatcacagc ttttgaaaca taagttttgt
      241 actgcttcga ttcgcccgaa ttcttttgca gttatttggc aatgagaaaa gcagacatct
      301 tctataggct tttcaggaaa tgctactaaa tcaaaagctc gggaaggaag tgagtaacct
      361 ggagaaaggc atgcctgtac gtttttgatg tgtatgtctt tgactttcgt catttgcttt
      421 gattcaggta tttcttcagc cactgcttgc caactttctg gcatttgagc aaggtcattg
      481 ctattttttc tattgtattc attatgccag tccattatcc aagaaaaagg aaattgtaca
      541 tcaatcatat tcagattttc taccctgata ttttcgatca ctccccctct ttcttttgaa
      601 gatttcattc gaataccaca gtctgactga aaaaaatcga tatcgtgaat atatacgtca
      661 ctaactccag cactaacttc gctaccaata gttacaccgt atcctgaacg gattttacat
      721 ctagcaacct cgattcttga tgattttcga ttgactcttg caccattgcc atctctgcca
      781 gacttaatag caatacaatc atcaccacag gccaaatcac attcatagac acgcacattt
      841 gttgaagaat caatatcaat cccatctgta cttggtccat cattgttgcg gatgattact
      901 ttttctacta atacatcgtt agaataagtg atttgcaagt tccaaaaacc tgatttctga
      961 aaaatcaaat cacgaacaac aacatgttct gcatgataca gtaaacaagc gcgtggacgt
     1021 ttaatagcat aatccgcaat ccaacgaagt cccttttgat cgtattcagc tcttgtcccg
     1081 cttttttgat cttttcccca atacagctcc caccaatggt ctccttgacc gtcgattatt
     1141 ccttttcctt ctataagaat atctttcgcc gagagaacat tcaaaatcgc tgctggccat
     1201 ttcatttcta cgccggctac tcgtgtatcg atttctggaa atgcttcgat atctgttctc
     1261 cccttgagga ctgcaccttc ttcaaagata agatgactct tatcttttaa aaaaagggct
     1321 ccgactaaat aaatgccttt tggaataaat acaggtaagt gttctttcgc accttgatcg
     1381 attgcttttt gcagtattat tgtatcatct tgttgagtgt cgtttgtttt aaagttcaaa
     1441 gcattgataa tccctttttt tgtccgcaat acgtttctcc ctatctttca ttgatttttt
     1501 tcaattcttc accaataaat acagtttttg aacgattcag tgggtaacct actagcaaaa
     1561 tgattgcgga tacaaataag cagaatgctg gtaacaagtt tgccatcgta taaatccttt
     1621 cttgtactaa aactgattgg atggctccac ctgttgtaga cgattggtaa ccaatgagtg
     1681 ccagcataaa tccgcctacc gctcctgcac atgcttgtcc tacttttctc gcaaaagaat
     1741 ttactccata tactgttcca tcttctctta agcctgtctt atattgatga taatcaatca
     1801 cgtcagtaat gaatgcccag accatcaaat tgaataaacc agatcctaaa gtagcgataa
     1861 ataaaagaat cagatatacc catgcatttg ttatatgaag gaaatacatc agcaagtaca
     1921 tacatgaaga agcaagtaac gcaaatacgc ttgcttcctt tttaccaaat aattttgtca
     1981 gctttgtagc aaatggtgct aaaactacta ctgttccgta agtaaataaa agagcaacag
     2041 acattgcttg tttattatgg aagtaatcat tgaataaata agtcatattc gtcccagcta
     2101 aaatctgatt gatcacaatg aaaatgtcaa caatgactaa aataatcagt gctcgattag
     2161 ctaacaaacc ttttaccaac tgagaagcag atactttttc gtttttgaca actcttactc
     2221 tttcagtagt caatttacaa gttaacatat atgcgacaaa tgctattgca gcacatccga
     2281 ttgaaatcca aaagaaatga ttccctgaaa cgacttgttg tccactcttg ttatgggtat
     2341 acattaaaat cggaatgaag aatcctgtag ttgatccgcc aatcgcagaa ccaatgcttc
     2401 ggaacgtgga caacgaggtg cggtcattag gatttgagct aattgctgct gccattgctc
     2461 catacgggat attgattgtt gagagaaaaa tcccataaac aatgtatgaa acaaaaatat
     2521 aagcaatctt caccgtcatt ggaagatgtt ggacgaatgg caaaaacaaa atgacagtca
     2581 ctaagcaaaa tggatacttc atttttctga tccaaggaat aaatcggcca gtttctgtta
     2641 actctgagac atcacataat cttccaacca tcacatcagc aaacgcatcc aaaattctcg
     2701 aaatcaagaa taacgtacct acgattgccc ctgaaacgcc aagaacattc gtataataaa
     2761 tcatcaagaa actattgact aaacctaaaa taaaacagtt acctagatca ccgaacatgt
     2821 agcctatctt atcggaaatc ccaaattcct ttttttcttt ttttattgta tttgctgtaa
     2881 cagcattttg tgaaagcatt tgaattcctc ctacctcttc tagtaaacgt tttcaaaatt
     2941 ataactcttt ttttcttgtt tttatattca caatgttggc tttttattgc attatcttga
     3001 cagataaaaa acaaaaaata atctaaaaac attgatgcat aagattgata acttttttta
     3061 cataacaata tatacaaaca aaaaaattca ctaactagtg aaaataagtt agtgaacctc
     3121 atttattaaa gaattaaatt acagatgatc ttttccatca tacttgaaaa actcaaaatc
     3181 agcataactt cctctttttt tatttagatc atgcactgct atcccgataa aatttccagt
     3241 aaaccctcct gagagtatac acatgttttt ttgcggacct actttcttcc attcttcagt
     3301 tgatacgtct ttcaaatgaa actgtgctgt ctctttcttt acagtgactt tcaaaaaagt
     3361 cttcttttct gtgagtggaa tcgtccaatc ttctatttgt gctactccgt tttctgattc
     3421 tatcatacgt aggcattttc ccttgtcctc attccaagta acataacaat aaatataatt
     3481 ggattcattt aagtacagta ccagtccagc catctgatta aaattattag gcatgtattc
     3541 aaatgctgtc tccgcatcaa aagtaaaatc cgtttgccga atagctaaca aatgatgttc
     3601 gaaggtagat tgaagagatt caccggaaat cagacgtaaa aaacctggtc tacattttgt
     3661 atcacaccaa tcagcatttg gcaaaatgcg tctggcattc cagttcttct ttagctctcc
     3721 ttcaaatgta tccataaact gatgatcttt ttcttgttgt acctcttcat ttgtctcaat
     3781 cattatttct tccatcggag ttgtctggct atctttaagt cttaaccaat cgtcggacgt
     3841 ccaatacact tcttgaatag cggtttctct tcccaaaata gccgcaccgt ttaatggtct
     3901 ggtacaaaga tatgtcatgt accatcttcc tgacggggtt tgtactaagc tcccatgtcc
     3961 tgtacattgc aatgtagaag caggtttgtc actagctgtc agcataggaa aaccaggatc
     4021 aagctcgtaa ggaccagtaa tattctttgc tcggcaaact gtaactgaat gtcctcttcc
     4081 tgtaccgcct tctgccgtca tcaaataata atacccgtta cgaaaataca aatgtggtgc
     4141 ctccgttttc gctaattctg tacctgcaaa tattttatag atgggaccat caagcttctt
     4201 cttctctgca tcatattctt gtaatacgat accgctggat ttattcggtg tgtccagacg
     4261 ataatcccat agttcattga caagccattt ttttcccgtt tcaagatcat ggaacaaaga
     4321 aggatcaaag ccgttactat ttaaataaat tggttcggac caaggacctg tgatctcaga
     4381 tgcagtgatc aaataattgt ggacatcttt aaacggacgg cctgcacttt tgacatctgt
     4441 gtaaacaaga taaaacaatc catcagcata actgatctgt ggcgcccata tacttccatt
     4501 tgtaggattt ccttgcaaag atacttgagt ggtcaaaata tccgttgtat gtgaccagtt
     4561 gactaaattt ttcgactcat atactcggat tcctggaagc cattcaaaag aggagacaat
     4621 aatgtaatac cgctctttca ctcttaatat attaggatct ggattgaatc ctcttaaaat
     4681 aggatttttc actttgatag tctgcatgat tcaggctcct tttctcattc ttactcgata
     4741 taaactgaat gccccagctg cgaaataaaa aaatgcaaaa ggaaacgcaa taaactgagt
     4801 gccaatcaac aatatgaatc ctgttataaa atacaaacca gtggcagttg ttaagtattt
     4861 tcctttagca gtcaaaaaca atgctactgc taaaaaagca agtgaaataa acaacacaac
     4921 ggcaaacaga ccagtcatgt cctgtaataa tccaaatgta ttttctaaag acaaagttga
     4981 atgatctaca gcaagcgact gataactttt tttaaactct gacgcagaca tattacttaa
     5041 agtaactgct aacccgccaa agaaaatcaa agatagactg ccaccgacca ttcctagcaa
     5101 tagttcccat tttcttttca tttctgaacc ccctgctttt ctaaatgtat ccattcaggt
     5161 cttttgatcg atgtttcata attgttatta cgttcagaga atttgacagt ctgttcattt
     5221 ttcttgtcat cccaattatc ccaaccagtt gggtgaacat gcccacctat cttacacgct
     5281 tcaaataaga catttgcata cggacgccac ggtcttccta aatagacgtt tctagtatca
     5341 ggttctgcag tcaaggtaca gctttcaaaa cgataccctg ttttttgatt tttcggcgta
     5401 gatgctgctg tgatgtatcc aggattttta ctggctaagc tatggatatg acactgatga
     5461 aaaacggcat ctgccccacc aaatatgaaa tccaccgttc cttcaatcca gcaatattca
     5521 taggtctgac gacaatactc aggattggca gaagccggtg tacaaaaagg agtaccatct
     5581 ttttgcaaat caggcaacgg acccgtgcat aaggtatcct gataaccaga tagtcgacag
     5641 ttttttaaag atacgcgatc tcctgaattg aataaagcaa cagcctgtcc taccttttct
     5701 cctggacctg ctgtattttc gatgtgaaga ttttctaata cgacattcga tccttctaag
     5761 aaaaaagtag cacttcggaa agtccctaac tggctaccgt caagatactc ctgttttgca
     5821 aaacgattac cggagataac tactgttcct gctcccacaa gtttaaaatt ggataatctg
     5881 ctttctatat attcatcata tcttccttct tttactatca agacctttgg atggctatcc
     5941 ggcaggcctg ccaaataatc gattccttct tggatagttt caaaatccgt cggggtatct
     6001 atagcattct ttccaattat cagtttgtct ttcatcctca taaatgctcc cttatgaatt
     6061 gtttccaaat atttctcatt tcttgtgttt ccatatgacc gcctgttaaa gaaaaagaag
     6121 aaaagttttc tggactaccc attgattgat actttttgtc cagccctttt ctcagcttca
     6181 gtacgccttc gtccaagcac atcgtatcgt tttttccaac taagctaaga cgagggcgcg
     6241 gagcgatttt ttcttggatt gctaacgttg tataagctgt caatagattt ggcacatagt
     6301 aataaaaccc atgatgatct aggcggcgat gtagcaaaag tatttcgatg tccacctgtg
     6361 ccgctagatc aatacaaact ttgacacgag aatccaatgc tgtcacccac cagcttaata
     6421 agcctcccat agaaagacca atcgttgcga gccgttgagt atctgtgtct tcccttgtct
     6481 ccaaataatc gatcaagctg atcacatcat aaatacgcat tccccataac gtttttcctg
     6541 ttaataaaaa ttgtttgaac aactcgcttt cagaaatacc tccgcgttct tcaaatcccc
     6601 aagcatcaat cgaccaaaca gcatatccca ttcctgtcag tacttctgca aaagatggag
     6661 aagctaagta aggagcacca tggatcaatt cacttttccc gactgaaaaa tctccgccgt
     6721 gagaatgcag gtaaaggacc ggcggaaacg gtccttcttt ttctggataa gcatagaggg
     6781 ctggtactgt ttccaattca tttaagtaca attgatagta ttcaagcata aaaccgtttt
     6841 gtttttctcg gtataatcgt tttccactag gggtatgtct gctagatgga gcacctaaaa
     6901 aatcattcaa ctgcataagc aaagttttta cgaacaacca gttcatccat attttctgat
     6961 tgatttgatt ggaatttctc aatactgatt tctttagtat tttctaatag aaatgctggt
     7021 ccctcgtgat tttcaatcga catctggtta aaggaaacct tacccgcaaa gccaatataa
     7081 aatccttgtc tgctcatttc ctctattcct gtcatcatag caggaacacc gggaactgca
     7141 tcttgggcca tcgagacatc gatattgtca aatgtgattt cttgtatgaa ttgttccgct
     7201 aatccataaa taaaacctgc agaagcatgg acattccttg ccgtgatatt cgaaaaatgg
     7261 atccttctga atgctggtgt ccgttcatct atcggataag cctttttttc ccatacatac
     7321 ggttctttcc ctcgcggacc acagaaataa taaagattca gtataaatgg acacatcaca
     7381 ttatccatga ctatattgga tactcgaata tcttcaacga tcccgcctcg acctcgtcta
     7441 gatttcaatc gtattcctcg atcagtttct tggaagatac aatttgaaat ggtaatatta
     7501 cgaatactac cactcatttc acttcctaat acgacaccgc catgtccatg gaccatcgtg
     7561 caatttgtga tcgtgatatt ttcgcaagcg atcctttcgt acgtatcttc agtccctgcc
     7621 ttgattgcaa tacagtcatc tcccacgtca atatggcaat tactaatacg cacatttttg
     7681 catgattctg gatcaattcc gtctgtattt ggtgaatccg ctgggttcaa gatcgtcaag
     7741 ttatcaaacg ttgcattact gcaaagaatc gggttgatcg tccaacttgg tgactggatc
     7801 aatttaatat ctttcacagt aattcgatga cagttatgga aactcatcaa tttaggacga
     7861 ggataagcga gattatctgg ttcatttcta aaagtatgcc accatttttt tccatttcca
     7921 tctaatgtac cgaaaccagt gacagagatg ttctctacat tttgagcata gatacaggaa
     7981 gcataaactt ttcgatggac tccttcccaa cgtgaatgaa caacaggata atcttttgga
     8041 tcgtcagaga atttcagaac cgctcctgcg cttaaatgaa gttcgacatt gctttttaaa
     8101 aataaagctc ctgttagaaa ttcacctgct ggaacaacta cggtcccgcc tccgtcactt
     8161 gctgcagcat caatggcttg ttgtattgct tcagtattca attcatcaat tgatgcgcca
     8221 aatgtcaaaa tatcatacat atttataatg cctccacttc aatcaatgct tgcaagaatg
     8281 caccaacgcc tttttgatca ttacaaataa ttggttcact gatgtagtaa gcatatgaac
     8341 catctcgctg atctgctccg cctaaccctg ctacttgaca gtttttattc aagttcagcc
     8401 agccttcttt tgtttccaga acaaattcat caagtaatcc ttgatgtgat ttcttcactt
     8461 gtggaagcca ttcttctttg ttcagcagtc ccaaacgtat tcctttagcc attgcgtaaa
     8521 caaacatact actgccggaa gcttccagat aatttccttt ccgttttgtt tctgttgtta
     8581 cttgatacca tgtcccgctg ttttcttctt gatatggaag taatgcagcc agtgtctttt
     8641 ctagaatttc tttcagcaaa gaacaatctg tttcttcatg aagtaattca taggtatctg
     8701 ctgcagccat caaataccag ccaatcgatc ggctccaaaa atgatgggat agacctgttt
     8761 ttggatcagc ccatggttgg accttctttt catcccatgc atgaatcagc aaatttgttt
     8821 ttggatcgaa tagatgttta taactgattt caaactgtct caaaacatct tggtaatctt
     8881 tttcttcact aaaattctgg ataaattcag cataaaaagg agaacccata tataagccat
     8941 ccaaccaaat ttgataagga taaatttctt tatgccaaaa agctccttcg gatgtgcgtg
     9001 gatggtttgc caactgctgg gaaaggagtt ctgctgcttt tttatatttt tcttcgcctg
     9061 tttctttgta caataaaaag aaaagctttc ctgtatttac atggtcgata ttgtattctt
     9121 caacgctgta gccacggatc gttccatctt cttgcacgaa atagtcgata ttgtctttta
     9181 tgaaatcaaa ataaattttt tcttgggttc gttcccaaag taattggaac cctttaagta
     9241 cgacgccata gtcataagac cattttcctt tataccactt ttcttcatat aagcgaggtg
     9301 ttctttccat gatacttttt gctaatttct ttgaccagtt ttccattatt tgctctcctt
     9361 taaaaattaa aataggattt gggacaaata ccaagaagta tttgtcccaa tcgtctatat
     9421 tactgtgccg ctgctttgta tgcttcctcg tactcttgtg tgattcgatc gcctccttgt
     9481 gctttccagt cttctacagc ttttttgaat ccatcttcgt caagctgtcc catgatgtat
     9541 tgcaccgtag catcaatgac gattttttca atttctgatc ctacttcatt gttcgtcggt
     9601 gaatccaatg gcacagaagg atccattaca gcaaattctt cattttcttt gatcaattga
     9661 ttagagaatt ctttttcagg gtctgtatct ttgaaagaga acgtcacttc actaggacga
     9721 gagcttgata acggttgcac ctcttgttgc caaagatcca tatcgataaa ttcgatagaa
     9781 ccatcatcat tcaattcata atgcgttcct tcgataccgc cagtcatcaa tttatacaca
     9841 tcttcttcta aaagatcatc aacaaattga agtaatttct tcaattgtgc ttcgtctttt
     9901 acttgagatt tgggaaatgc tagcaagccg ccaataccat ttccttctga ccaaacatga
     9961 taaccgccgc cttcaccatc tgagatcttg tttacaggca ctaattccat atcatcttga
    10021 ataccttgac ccatcgtctt cagattcttg atatccacca ttccagtgta gattccagtt
    10081 cttccttgtg caaattgttc ttgctgatca gtcttttggg taactgcaaa gtcttgtgcc
    10141 aaatagccat tctcaaatag atctcttgaa aaattcaaag ctttcatata ctcttctgta
    10201 tcaaattctg gagtgaattt gccattatcg tctacagccc agccatttgg tgctccaaaa
    10261 taagaagtga aaagtttaaa gctggtataa cgaatatcac taggcgcacg atcgccaaaa
    10321 ccaactgtgt cgtccacacc atttccatca ggatcgtttt ctgtaaattg tttagctact
    10381 tcataaagct catccatggt agttggaaca tctagtccta gattatccaa ccagtctttt
    10441 cgaatgacta agccagcacg agcgtagtct ttttgtatcg gtacgccgta caaggcaccg
    10501 tcgatactcg cagcttcgat tctatcttca gaaatttgac ttaaattttt atagtctttt
    10561 aaatattctg atacatccca gaatacgcct gattttaacg cattgcggac agaagagttt
    10621 ttcaacatcg caatttgaag tgtaacgata tctcctagtt catcagaagc caatgcagtt
    10681 gttaatcttt cttcttttga agcatcagga atccagttga atgtcagttc cgtatcggtt
    10741 ttttcttcga tcaagttcac aatcgtgtct gtaggcggtg aagcatgatg caaaatattt
    10801 aaccaagtga ttttagcttt tccatcttct gtctctgaag aactgttact tgaactgctg
    10861 ccgcatgcgg caagtgctag aattcctgca aaactaagac ctaattttac gagcgttgct
    10921 tttttcataa attttctcct tcactgtttt aataatattt ttgttttctt ctgttttttc
    10981 tgtatatgag ttcttccatt ccaaatggca aaatatagaa tttgctgtcc acgtgactgc
    11041 ggaaaagaag agactgccac cgataaaggt taaaacacct ggcattgttt ggatgatcat
    11101 cagataaatc cctaccatac ttaatatcaa cactaatgag taatgaagat gtgagaatcc
    11161 gaataaaaat gccattttca ttttgaaaaa atatcctttc caatcataat gagccatgat
    11221 tgggaaaata tagagtgctg agagcacata tatgaacgca gcaatataca aagcgatcct
    11281 aagataccaa ttgtgtatga aagaaagatc cacaagtaaa acaatcccga tggcgccata
    11341 tatccagctc atcagcaagg tttcacgata gttttcttta aaatatgtga agtacgtttt
    11401 cgtcaaagga aacgtttctt ttgagcgcag ccattttcta atcactgcaa ccatcgcata
    11461 cgatgcagga ccgaaagtaa aaactcccaa acctgcgatg actaaaccgc cccacagaaa
    11521 attcaaataa accaactgta aaatacgatt tagccttccg tttattttat ctaatgactg
    11581 cagcatatct ttccctctct ttctatctta gtaaacgccg tcttctccta gctttttggc
    11641 aatcttatta gctaaaatta ccataaacaa tcctacgacc cctttaaaca atcccattgc
    11701 tgtactgaaa cttaactgtc cattcttcaa tccagctgta taaatatagg tgtcgaaaat
    11761 ttctgcaacg cttcgattca atgaattcaa taacaagtac atatgctcga accctaagtc
    11821 taatgtctga ccgattttta gaatcagcaa ggtaatgatt actggacgaa ttgccggcaa
    11881 tgtaacatgc catgttttgc gtaagcgtcc tgctccatcc atttctgcag cttcatacaa
    11941 ctgtgtatcg actgcagtta tcgctgaaag gtagatgatc gtagaccagc ccaactcttt
    12001 ccagatcact tgtccgatat atactgtgcg tgtccattca ggggatgtta gaaaactgat
    12061 tttttcaaat cccattgttg caaggaattc attcaatacc cctccgtcaa cattcaaaaa
    12121 tacataagta atcgaaacaa tgattaccca agacatgaag tgagggatgt aaatgatcgt
    12181 ttgaatcccg tttttcaatc gtttgttcgt tacttcattt aatagtaaag acaaaatgat
    12241 tggcatcgga aaaaacacga agatatttaa tccgaacaag atcaacgtat ttcttaataa
    12301 tgtaaagaat gtcggttctg taaacaaacg aatgaaatgt ttgaaaccga cccaaggact
    12361 tcccattatt cctaaaaatg gttgataatc ttgaaaagca ataacgagtc cccccatagg
    12421 aagataccta aaaatcaaga aataaaggac tcccggcaga atcatcacat ataaaaattt
    12481 attcgttttg attcctgcaa ttattttttt tcgtcttctg agcttttctt cttttttcga
    12541 tagcgtcagt tgaaatggtt cttgtttctt tacactttcc ataagcccct ccttagtttt
    12601 gtgaatgttt gctgcttatg acttcatgat agataactat ctgaattacg tatataatca
    12661 tttttaccgt aagtaaaaac gcttacatag caatgatttt tgtgctatac aaaacgataa
    12721 agtgtatttt ttcacaaata aactacgcat agcaaacctt ctattttttt ctatctgaca
    12781 cttagcatag gaaatgatta tatacgtaat ctttcaccga tgataaattg aacatataga
    12841 aacagaaaca tttaatttat tttgaggagg agcctatcta tatgaaaaag aaagtctcta
    12901 taggagaaag aagttttaca cttttcaaca atacgtttct aattttgcta gctttgatct
    12961 gtatcgttcc ttttttaaat atcatcgcta catcttttgc ttctacacaa gaagtcgtcg
    13021 cgaaaaagtt tatccttttt cctactacct tttctttaga cgcgtatcgc tacattcttt
    13081 ctacgccgac catttttaga gcacttgctg tttcgattgg tgttacaggt gtcggaacga
    13141 tcgtcagtat gtgtgcaact tcactgatgg cctacggtct ttccagaaag tatttgtttg
    13201 gtcgaggttt cgtcaacttt cttgttgtat tttctatgtt gttcagcggg gggatgattc
    13261 caacgttctt agttgttcgt tctctagggc tggtcaattc ttactgggcc atgatcctgc
    13321 ctgtggctgt taatgcaatg aatatgatca ttatgagaaa ctttttccaa gccttgccgg
    13381 atagtttgga agaatcagca aaaatggatg gttgcacaga ttttggtgta tttttcaaaa
    13441 ttatgctgcc attagcgtta ccgtctattg ctactatctc gcttttttat gctgttactt
    13501 actggaacac ttatatgact gctattttgt atatcaacga ttcatctaaa tggccgatac
    13561 agattctttt acgccaaatc gtcattgtat caagtgggat gcaagcagaa agtagtgccg
    13621 ttgatgtgat tccaccagca caaacagtta aaatggctgt tatcgttatt gcaacagtgc
    13681 ctatgctgat tgtttatcct tttgttcaga aatattttgt caaaggagca ctcgtaggtt
    13741 ctgtcaaagg gtgattatgt actgaacgca aaatggtagt tgtgtcagaa gtggtcagct
    13801 tcaagaaata agaaggcatt tacgaaaatt gcttcttaaa tttttgtgaa atttcggctt
    13861 atttttcgaa gaatctactc ctggaacacc gtgtatcaga ggttgtgaaa gagacgttca
    13921 gccttgagcg atgataaaaa ctctcacaaa cattgcgacg agtaacccac aggagcaata
    13981 agaagcgatt ttgaaaaata gcttcccata tttcacaaaa ttatgcctta ttgtcgatag
    14041 cctgtctctt gaacgccatt tattcgtaaa aaagaactgt gacaagactt ttgtcacagt
    14101 tctttttgcg tgcttagcct tgaaatttaa ctatccataa atacaaatac tagggttatt
    14161 ctaacttctt ttattgcttg aatcagtcca caagaaaaac agcctcttga ttcacaaata
    14221 tttttttcga tattctcccg gcgtaatcga ttccttcttt ttaaagaaac gaataaaatt
    14281 ttgcggattt cgatattgca atctttctgc aatctctttt acggagaggt tagtctcttt
    14341 caaccatttt tttgctacct ctaaacgatg attttgaaca aaatcaccaa atttttctcc
    14401 tgtttccttt ttaaatacat tacttaaata gttagggtta taatgcaacc tatcagcgat
    14461 cacatctaaa gacagatcac gatcatattc tgtgtagacg atatgcataa tattctccga
    14521 caaattttta aattcatgat cagtgattgc ttgagagcat tctgtgattg gcagaactaa
    14581 ttgttctaaa acaagttttt ctagttcttt gggatgataa ttgttcagtg cttttacata
    14641 aatttgtttc atgttatcaa agttttttga atctactccc aataactgtc ctaattgaac
    14701 gagttcattg atcaagcgga ccgaaactac ttcccgactc aaaggatttt tattttgtgc
    14761 aaacaatgca tcaatcaatt catgtactaa attcttcact tcctgatttc cagaacgaat
    14821 ggcttcaaac aatcgatttt gttcttctat cggatatcta ataagtgttt tttcatgagc
    14881 agcaggaacg atttcctgat aaaaaataat tgaacttgga ccagtattta cacgataata
    14941 cagggcttct tttgcccgat caaccgattg cttgctttca ttcagcgttt cgaaccggtt
    15001 actgatgcct atactgatag ttaaattcaa gtattctttt acacgttttt gaatgagtcg
    15061 gcaatactcc atgacttttt tgcttgcttc tgcatcatct ttatccattc gataaatagt
    15121 ggcttgcatt tcttcattca aaacgatcgg cagcattcga ttttcctttg gcacaatttc
    15181 cgtgatcatg ttgttgatcg ctaaaagtag caaatcacgc tccccaccgt gactatcttc
    15241 taacagatcg atttgaacca aagctgtata atagcagtca ttttgagaac tataaccaaa
    15301 ttgctgcaac cgctgatcta gttctcgcct ttctactcga tttctaaata aactaagtac
    15361 aaatagcgtt tccagctgtg gcttttgggt aactaagtgt gcacttaacg cttctttttc
    15421 tcctatgatc cgactgactg attgagcgac caaatctagt tctttcccac tgaatccttt
    15481 tgatttaaga ttcagacgtt cctgtatttg agaaatcgga cgggcaaatc tgtccgaaaa
    15541 taagtaggaa agaagaccaa tgagtcctac cagtgttaaa gacactgcaa ctagcccgat
    15601 tctcagattg cggatcattg cgccaattgc actagagtca acttctaatc tgtaaatcca
    15661 attattataa tctgatttca gtactatata cgttttatct ttttctctta tcactttcag
    15721 ctgattgtaa tctagatcca cgtccgttaa tacagttggt tgctctttat tttttactgt
    15781 ctgagtagta aaaaggttgc caacttcgtt ttcaatagaa agtatgtctt ctccactttc
    15841 accaacaact tgttctattg tatgtttctc tatcgtagca attcccaatg catatttttc
    15901 tttttcaaac attggcaaag aaatcaccat ttcgattcct ttgtttttct tttgccaaaa
    15961 taaattattt ttgttattta tgtacttctg ttgatacttt tcaatttcct cttcggtcag
    16021 ttgactcaac gatccattga ttactcccca tttaccagcc aaactgacta aatcataatt
    16081 attatcatcc ataacaatcg tttctataaa atttagttct ttcctgattt cagaataagt
    16141 ggagtaatct gtatatgtca gcggatgctt gaatatgtcc gagaaatttt tggtcgaacc
    16201 atacgtagaa aacgaatatt caatcgttct aattttccat tcaacgttag ttttaacttg
    16261 cctgagcaaa ttctttttgt ctgttaaata actcgattcg atcgattcat aggtattctg
    16321 gtaaatgaat aaactaaatc ctatgacaag tacggctcct aataaaaaca tcggtaaaaa
    16381 tattttataa aatactgtac ttttcttcat tttcttcctc tcctcctttt aaaataaaag
    16441 gctttcattt ttctgtaaaa ttgatactca tctaattaaa tgagcattcc ccgctttcaa
    16501 gaatagcata attaaaaata aaaatccaag taatttttaa cgtgtgcaat cttttcatat
    16561 caaagatcta ttgacttatc gtagttatcg taatatgatg aatgcgttaa caaaaagtga
    16621 aaaagtaagg aaaatgatgg aatggtaacg atcaaagatg tcgctaaagc agctggcgtt
    16681 tctcatacga cagtttctcg tgctttgaat aacagcagtt taataaaaaa tgaaacaaaa
    16741 caaaaaatac gacaactagc taaggaaatg aattattcgc caaactatag tgcaaaaggt
    16801 ttagtgaatc aaaaaaaata tctaatcgga ctatttttct caagtttgca taaaggaact
    16861 tcatcaaatt tcctagcaga ttccattgca ggcatccatt cagtattaga cacaaattac
    16921 agtttatctg ttgaaagtat cgatgttgtt gatttaacag aaatcaacct gcaacgctac
    16981 gatggactga tcgttatgag tcaaagtgat gatgatcagc ctttcatcga ctatctcaaa
    17041 gaaaaagaat ttcctttcgt tgttgtcaat cgtcacatca atgatccagc ggttatcaac
    17101 gtcactgccg atgatgcggc cggtgttatg caggcagtag attacggtat tggatgcgga
    17161 catacgaaga tcgcttatat tggcggaaag aaaaattttc gttcgacaaa agaacgggaa
    17221 aaaggggtat tgaaaagtct aaaaaaagcg agcatccctg tcaatccagc ctatttttta
    17281 acaggagatt acagcttaga aagcggcctc caaaacatgg aatacctatt atcactttct
    17341 gacctgccta ctctggtttt ttgcggcaac gacgacatag cgattggtgc attgagagct
    17401 gtacatttag ctggcctccg tgttccccag aacatttctc tgatcgggtt cgatgattct
    17461 ccagtcgtct cctatctcaa tccgccgctt accaccgtac gtaagccttt gaaagaaatg
    17521 tgtcaagaag gaacaaagct tttactagat ttgatcgaag gcaggacgat cgaacaatct
    17581 aaaattgagc tctccacctt gttgatcctt cgtgagtcga tcaattactt gtaaactaca
    17641 agtaatttta tataaaaatg ttaacgtgtg cataacacag aaaggaagtt tatatgaaaa
    17701 aactgacaaa agaattagtg aaaaaaaacg aagcacctgt aaaagttctt cagtttggtg
    17761 aaggcaattt tatgcgtgga tttattgatt ggcagattca gcagctgaat aaccaacaat
    17821 tgtttaaagg taatgttgca atcattcaac ctttagaaca aggtcttgga aatatgatga
    17881 accagcaaga taatttatat acggtcattt tacaaggact cagcaatggc gaaatcgttg
    17941 atacttccga gattatcacc gtagtagaac aaatgatcaa tccttatgat gaatgggatg
    18001 cttatctatc tctagcagaa aatgacgact tggcctatat cgtctccaat acaacagagg
    18061 caggaatcca attcgtggag actgataatt taacagatac tcccccacag agcctcccgg
    18121 gcaaactgac agccttttta taccgacgtt tcaaattaaa caaagctggc tttaccatca
    18181 ttccttgcga actgattgac agaaacggcg aacgtctaaa agaaatcatt ttacgctacg
    18241 cagaaaaatg ggatttagaa cctgcttttg ttgagtggtt agaaaaagaa aatgttttct
    18301 gttgtagttt agttgatcgc atcgtcccag gttatcctcg cgaagatgca ggtgttttca
    18361 atcaacggca taactatgaa gatcaattga tggtcaaagc agaaccattc atgctttggg
    18421 tgatcgaagg gcctaaagaa ttggagaatc aactaccatt caaacaggct ggcttgaatg
    18481 tcatcatcac agatgatatg actccttatc gtgaacgtaa ggtgcatttg cttaatggac
    18541 cgcatacagc gatggtccct cttgctttgt tggcggaatt ggaaactgta gaagaagtaa
    18601 tgaaagatcc tttattctcc gcatatgttg atcagttatt caatctagaa ttgattccta
    18661 tgctttcttt acctaaagat gaattagcca tttatgctga ccaaatcaaa gaacgtttct
    18721 tgaatccttt tgcccatcac aaattagaag cgatttcatt aaatagtgtt tcaaagttca
    18781 gcacccgtct gcttccagtg ttcaagaagt atatcgaaga acaaaatcag gtaccaccgt
    18841 taatcactgt ttcactagct gcgctacttc ttatgtatcg aggcgatcaa gtaaagccac
    18901 atgatgatga aaagacgata agcgaattta ctgatgcttg gtctgataat ggaacagcta
    18961 ttcctcgatt attgcaaaac gctgcgcttt ggggagaaga tttgtctcaa ataccgaatg
    19021 tgacagatac agttcaagaa atagctgcaa agatcgatca aaaaggtgtc cggtcagtat
    19081 tacaagaaat gaaaggaacg atggtccatg cttgaaaccc tgcaaccaac aatgaaatta
    19141 aatcgacgag acagtgtggt cgtcgcatta acccctattc caaaacaaac acaactgacg
    19201 attgatcagc aaacgatcac cactcttgaa gatatcccgc aaggtcataa gattgcattg
    19261 ggtgatttga aagctggaga caacgtgatc aaatatggct atccaatcgg tcatgtgacg
    19321 acagacgtaa ctgccggtca atggctgcac actcataatg taaaaaccaa tctttctggt
    19381 gaattggatt atcgatatga aaaagatgtc catccaagtc attacctttt tgagaaccga
    19441 acatttcaag gttacttgcg aaaaaatgga aaagtaggga ttcgtaatga tttgttttta
    19501 gtacctaccg ttgggtgtgt gaatggcatt gctgaactga tcgtcaaaca gttcaaggaa
    19561 aaacatccag atctgggaca gtttgaccat attacgattt taaagcatcc ttatggctgt
    19621 tcgcagcttg gaaaagatca tcaaaatact cgagaaattt tagcggacgc agtaaatcat
    19681 cccaatgccg gaggagtttt agtatttgga ttaggttgtg agaacaatac agttcctgaa
    19741 ttcaaaaaaa ttttaggtga atatgatgaa gaacgagtga aatttctagt ggctcaagat
    19801 gtttacgatg agatcgaaca aggtgtggct ctattagaag agcttttaac agcggcggaa
    19861 aatgaccagc gcatatcagt cccgctctct aagttgaatg tcggtttgaa atgcggcggt
    19921 tctgatggtc tatcgggaat aactgctaat cctttacttg gtgcattttc tgactacttg
    19981 attgctcaag gaggcagcac gatcttaaca gaagtgccag aaatgtttgg cgcagaacaa
    20041 gtattgatgg cacgagcgga aaatgaagaa gtttttgact caatcgttga cttgatcaat
    20101 gacttcaaac agtatttctt gtcttacggg gaaccagttt acgaaaaccc atctcctggt
    20161 aataaagcag gtgggatcac tacattagaa gataagtctt taggctgcac gcaaaaatca
    20221 ggaagctctc ctgtcgttga tgtgctacaa tatggcgaaa aaatcagaaa accagggctt
    20281 agtttattgc aagctcccgg gaatgatctt gtagctgcct ctgctcttgc ttcatccgat
    20341 tgccagttgg tcttgtttac cacaggaaga gggactccgt ttggctcata tgtcccaacg
    20401 gtcaaggtat ctacgaatac aactcttttt gaacgcaaag gtcattggat ggattttaat
    20461 gcaggcgtct tattagaaca accaatggaa gtaatcttag aatcgtttgt tcaaaaaatc
    20521 atcgctgtag ctagtggaga agaaacgaaa aatgaacaaa atgacgttcg agaaattgcc
    20581 atttttaaaa acggagtaac actttgattt tcataaaaca gtataaaaag aaagaaggaa
    20641 gatttatgtt tttatctgat gattttttat taaaagacga ttgggcgaaa aagctttacc
    20701 attcctacgc aaaaaacatg cccattattg attatcactg tcacttagat ccaaaagaaa
    20761 tttacgaaaa caacaatttc agcaatctga cagaagcttg gttaggaggc gatcattaca
    20821 aatggcgctt gatgcgagct tgcggcgtgc ctgaagaaaa aatcacagga aatgcttctg
    20881 attttgaaaa atttttagct tggtgtcaaa ccgtaccaaa aattatcgga aatccgcttt
    20941 acagttggtc acatcttgaa ttgaagcgtt tcttcggtat tgacttgttg ataaatgaag
    21001 aaaatgcaga gaaaatttgg acacaagcaa atgaaatttt ggcagctcct gattttcgcc
    21061 gtcgggaaat cgcaaaaaaa tcgaatgtag aagtgatttg tacaacagat gatcccgcag
    21121 acgacttgca ctatcatcag ttgcttgctc atgaagaacc agatttgaag gttctacctt
    21181 cattccgtcc agataaagca ctcaatattg aaaaagaagg atttaatgca tgggtcacac
    21241 tactggaaga agcagctgac caaacgatca ccacctatca agaactgata gaagtcttag
    21301 ggcagcggat agaatacttc catcaacatg gatgtcgtgt ttccgaccac gctttagaca
    21361 tcttacgcta taaagaagcg gatgaaacag tattagaaga gatttttcaa aaagcacgag
    21421 ctaacaaact attatcttca gatgaaatcg atgcttatcg tacagaaaca ctgatacatt
    21481 tgacccactt ctatcattct cataattgga cgatgcagct gcacatccat gcttatcgta
    21541 actgtaatac acaaatgttg gaaaaattag ggcccgatac cggctatgat ggaatgaatg
    21601 atctttcttt gactattcct ctgcaaaaat tattgaaccg tgcagaagaa acggatcagt
    21661 tgccaaagac gatcctttat tcattgaatc cgaatgacta tcctgcgatt ttagctttga
    21721 tgggctgttt ccaaaaagaa cagaatggaa aactgcagtt aggttctgga tggtggtaca
    21781 acgatactcg agctggtatg cgcgatcaat tgacacaatt tgctgatggt agtgctttgg
    21841 ggaattttgt cggcatgctg acagattcaa gaagtttcct gtcctacact agacatgaat
    21901 atttccgtcg tgttctttgt gaatttttag gcgaaatggt catgcgagga gaagcacctg
    21961 aagatgaaca acttttagga agtctagttc aagcgattag ttatacaaat gctaaaaatt
    22021 actttggctt ttttgcctaa ttgattaata gtaacagtaa cgaatcaaaa acaggatagt
    22081 gaaataacgg aatccgttta tccacgtttt agagaagaag acggaccaaa tttgctccgt
    22141 cttcttctct tgctgttctg cttcattgcc tataaacctt tatgaaagaa gagataacat
    22201 gcctactatc catattgccg gtgattctac tgcggcagaa aaagatgaaa acaagcgtcc
    22261 cgagacaggt tggggagaaa aaatctctgc ttatttttca cccgagatcc agatcaataa
    22321 ccaagcaaag aatggacgca gcaccaaatc atttatccag gaaggaaggt tagcccgtct
    22381 agcttcacag tttcagccga atgatttctt atttgtacaa tttggacata acgaccagaa
    22441 aatccaagaa gaaagaggta cgcttccctt cggtagctat ttaaaaaatc tgcagatatt
    22501 ttttgaatgc gcaaaaaaag cgaacgtcca aatggtctta ctgacatctg tgacgcgacg
    22561 agattactta gaaaacggaa cattgaatcc agatatactt ggagactatc caaaagcgat
    22621 gcgtgcattt gctgagaaac accatattcc tcttttagat gtgttctcaa gatcacaaga
    22681 attgttccag accttctcaa aagaagagac aaaaaaattc tacctgcatc ttatacctga
    22741 tacttgtaaa aattacccag aaggactaaa agacaatacc cactttagtc cagaaggcgc
    22801 tgataaagtt gcccgaataa ttgttgaatt gataaaagaa tcccgtctgc ctcttgctaa
    22861 ttatttacaa aaaggagttt gagatgtttc cattaaattt aggcatccgt gcccacgatt
    22921 tagatgttcg aaatcgtgaa gaaatcgttc aaaaagtatc acagcttcac ttgtcccata
    22981 tccaatttgc accacaaaaa gcatttcctg aacaacttaa attaactgac atgacacttg
    23041 ggagtgctgc ctattttggt gaatacttcc aaaaaaacaa tatcgaactt tccattttag
    23101 gatgctatat caacctatcc agcaaaaatc aagaagttcg tgcacaagca ttggcaactt
    23161 tcaaacagca aattgctctg tgctctgcct atcaagccaa gatgattgct acggaaaccg
    23221 gtagtgtctc tgtcggttac acaaaagaaa attttactga agaagcttat cagatcgcca
    23281 gaaattctat cattgaatta gtcgctcatg cagaaaattt tggcgtaact gttgctgttg
    23341 aagcagggat caatcatcca atctataact atcagttagc taaacgttta atcgatgaag
    23401 tagcttcacc aaatttaaaa atcattttag attgtgcgaa tttgatgcat aaagaaaatc
    23461 atgctgatca ggaaaaagtg atccgaggtg ccttggacaa tttacatggt tatattacag
    23521 cgcttcatct caaagactat atcatggaaa atggaaagat ccgcatggta cctgttggca
    23581 agggctgttt agatttccgc ccggtactgc attatatcaa atatgagcgt cctctaatgt
    23641 acgccactct tgaagctact cctgagattt atgttcctga agcaatagaa cacttgcagt
    23701 ttatttacca agattgttaa ggagaagatt atggaggaac gaatacagaa aagttatcaa
    23761 aaatatcatg caaatgcttt tcagacattg ctgcatcttt atcagggaaa ttattggcgt
    23821 tttgccatgt caggactctt ttttattatc aaacatcttc catcttggtt aatgccgatc
    23881 gctattgcca atgtaatcaa tgcagcttca tcgaaagatt cttctcagtt gcaggccatt
    23941 tatttcaacg cttttttgat ggcttgtctg attggtcaaa atatattgac gaattatttt
    24001 catgtaaaat accatagcac ttcgatccgt caagtagaag ctggactaag agcgaccctt
    24061 atcaagaaaa tccaagaact atctattcct tatcaaaaag aattgcaatc tggacgaatc
    24121 cagtcaaaga tcatccgtga tgtagaagct atccaaacgt tgtcctctca agtatttgtc
    24181 agcggattaa atatcgctgt caatttattc gtagctttag gtatcacgct ttataaaagt
    24241 cctgttgttt tcatgttttt cttattgact gttcctctcg ccgcattcgt tactctcttt
    24301 ttccgtaaaa ggatcagaca gtccaactca gacttcaggc aagaaatgga agagacttct
    24361 gcaaaagtga tcgaagtgat cgaattagca ccgattgctc gagctcatgg gttagaaaaa
    24421 cacgaaacaa ctcgcttaaa taagcatttt actcaaatct atgaaaaagg attccactta
    24481 gatatcctgc aatctgtttt cggatcagtc agctgggcta gttttcagat atttcagctg
    24541 atttgtttga ttttcactgg gatattagct ttacaagggc gcatccaacc cggcgatgtc
    24601 gtgatgtatc agacgtattt cacgacgatc gtcaattccg tttctggaat gatcacgctg
    24661 attcctacga tttcaaaagg catggaatct atcacttcta ttggcgaact gttgattgct
    24721 ggcgatgtag aaacttatca cggtaagaaa aaaatccaac atttggatgg aaattttcgt
    24781 tttgaggatg tttcttttct ttatccggac tcactcaaac cgattatcga tcatttttct
    24841 ctagaggtga aagctggaga aacaattgct tttgttgggc catctggctc tggaaaatct
    24901 acactcctta acttattgat tggatttatc cagccgactt caggaaaaat ttttctagat
    24961 gatcagcctt tggatgaact ggatatgcga agtgtgcgaa attatttagc cgttgttcct
    25021 caaaccactt tgttgttctc cgcttcgatc aaagagaaca tcacttatgg cttgaaaaac
    25081 gtatcaaaag aacgtttaga tgaagtcatc gaagctgcgc aactttcttc actgattgac
    25141 cagcttcctg atggtctgga taccttagta ggcgaacatg gcaacaagct gtctggtggc
    25201 caaaagcaac gtatctccat tgcacgagcg ttgatacgtc agccaaaaat cattttgcta
    25261 gatgaagcaa cctcagcttt agataaccag tcagagaaaa aaatccagca agccctgaat
    25321 tacctgacag aaacacccac tacttttatt gtggctcatc ggctttcaac gatcaaagag
    25381 gcagataaaa tcgttgtgat cgatgaagga aaaatcgttg aaataggtac ttacgaaaaa
    25441 ctgttagctc aaaaaggcta tttctaccgc ttgaaaaatg aatgacaaaa aataggagct
    25501 atgacaagac ttttgtcgta gctcctattt ctgaataaac ggtgttctcc tgccaactcc
    25561 tcggtcacaa gtcacaatag tcatcaaaca tgagaagctg ttttttgaaa ttgttccttg
    25621 taactcagag ttaaacggta ggctcacgac ctcttggtaa atggtgttca agagacagcc
    25681 tatcggcaat aagaagcaat tttgtcatcg ctcaaggccg aacgtctctt tcacaccctc
    25741 tgttgtttca taaaatagtt ttattcgctg taaaggtcgc gaactggtgt catgatgata
    25801 cggttcaagt cattgatgat tgtactgaag ccttgctctt tttgcatcaa gtcattgatt
    25861 acttcttctg attggatttt cacagccatt tcttgtgctt tcattgcatc ttcgtcgctg
    25921 aactcttgtc cactcatttg tttttcttgt aattcgattt gcatcgcttg gaagtctttg
    25981 aacagtttgt gtgcttcttc gtttgcttgt actttctcgt acgcttcttt caacgctttg
    26041 aattctggta attcacggat ctcacgttcg atttgattcg cgctatcgta gatgtttgac
    26101 atgttatgcc ctcttttctt ttagtagtta ctctctattg taacatgggg ttattcgttt
    26161 tcaaaggggc tactctcctt gatatttccg ccagaggtct ttagccgctt caccaattcc
    26221 ctgaacgcct tctttcacca tatcgccgaa gtttttcagc ccttcttttg cttgttcacc
    26281 atattgattc aagttatctt gccaatttcc agactctcca ttagaatttc ctgtgtttcc
    26341 aacttgatct gcagctacca cttgtcctcc tgtggcatag gcatctgcta cactaaatcc
    26401 tgtttttgaa gtatacggaa ggatgccttc tgccgctgat ttgaagactt tcccgacaac
    26461 atttccactt gtcccttcca aatagtgcag cttgctggat tcttcgtaac ccatccatgt
    26521 cgaaatcact acgtctggcg tgtaaccgat catccattgg tcatttgctt ttgtggcatc
    26581 gaaattagtt tctgtggttc ctgttttccc tgctatcgta taacctgcag gattcgcagc
    26641 agccgctgtt ccatttgaaa atgtaccaag aagcatgctg gtcatttcat cggctgtttc
    26701 ctttgatatg acctgttttg gttttttgtt tgtgttatcg acgatcaccg ctccagtcga
    26761 atcaacgatt ttcgtgataa gatgcggcgt atacatctga ccatcattgg caaatgcact
    26821 gtaagcaccc gccatgatca atggagatac tcctttttcc agtccaccaa gggctaagcc
    26881 ataatactta tcagattttg ctaaaggcag gccgaattct tctgctttat catatccttt
    26941 ttgtaaaccg atctcatgca aaagccatac agtaggcaaa ttcaaacttt gtgcaacagc
    27001 ttgatacatc ggtacttctc cttggtatgt cccgtcaaaa ttatgcgcat cgtaataact
    27061 ttgcggttcg tctttcaaga tactggaagg cttgtatcca gcttccagtg ctggtgtata
    27121 tacgcttaat ggtttgatcg ttgatcctgg tgaacgacgc atctgtgtag caaagctgaa
    27181 tcctctaaag acatgatctc ctcttcgtcc tactagtgct tgtacaccac ctgtattcgg
    27241 atctaaagct actgaagcag attggaccat ggcaccatct gaagcatttg ctgggaataa
    27301 tgcactgttt tgataagtag cttctaattg cttttgataa tcctgatcta aagctgtata
    27361 gattttatag cctttattca tgacatcttc ttcatcaaga ccatactcgt taactgcttc
    27421 atcgatcact gcatcaaaat aataaggata acgataacca gaatctgaat tatcgtatgt
    27481 gtcattaaga tacgcgctga tatctgtctg gctttctgct tgttcttgtt cttttgtgat
    27541 ttttccgttt tcttccatta cacttagtac tgtgtttttc cgatcattcg cattttttgg
    27601 gtgatcgatc ggattataaa taccaggacc tttcagcatt cctgccaaag tagcagcctc
    27661 agacagcgta acattcgttg catctactcc aaaatacttt ctggaagcgt cttggacacc
    27721 ccataccccg ttgccaaaat aggcattgtt aagatacatg gtcaagattt cttctttgct
    27781 gtacttcttt tcgatctcga ttgccaaaaa taattccttc gcttttcgat caaacgtttg
    27841 gtctaatgtc agatacgcat ttttggcaag ctgctgagtg atcgtacttc ctccgccccc
    27901 tgatgttccg ccagacaaga ccattcggac tgctgcacgg gcaatccctt tgatatcaaa
    27961 cccgtgatgc tcgtaaaaat tcctatcttc tgtcgagatt actgcatctt ggatataggg
    28021 agaaatcgcg tttaattcta catatgttcc tttttgggcg tacagtgtgc cagcttcttc
    28081 gtttttctta tcataaatga ctgtcgattg gcttaatcct gattttaacg tttcgacatt
    28141 tgcttgtttt gctaaaataa agaggtagat acttgtcact aagacacaga tcaaaccaat
    28201 cagcaaaatc aatttgttca catgatattt tttccagatg cgctttcgcc atcgatggaa
    28261 ttgtccgaga tatggtctga tccaacgcca aaaactgatc aaagcagctt ttatcttttc
    28321 taaaatgctt tgaaaatcca tttgcttctt ctcctgtcta tttgattcaa tcgtcttatt
    28381 ttaccatagt gaaagagtgt tttcttcctg cttaagttgt agttcaatat gtttttttac
    28441 gttagtgcaa gtagagtaac ataagaatga acagaaaaat gtaagttttt tcttaaatat
    28501 gttttaaaaa tggtacaatt agtctgcgat tgcactgaaa ggagtacact tgtgaaaatc
    28561 acgatcactt tacctatgaa ccatcctgaa acaacaattc gcgacttgct tgaaaaggaa
    28621 tggttagttc ctagaaaggt tcgacacttt ttgcgtatac gtaaaaacgt ttggatcaat
    28681 caacaacctg ctttgttcca tcatgaagta aagggcaatg accagatcac attgttattc
    28741 gaagaaacag attataatta tcaagaagtt caattaggag atgcgaagca catccgtgtt
    28801 ttatatgaag atgagcattt gattatagtg gataagcctg caggaatgaa gactcatcca
    28861 aatgaaccga cagaaaacag taccttactc aatcatttag ctgcttatct cgacactaaa
    28921 cagcaacgac cttacgttgt ccaccgcttg gacaaagaaa caagcggtgc cgttttattt
    28981 gcaaaaaatc catttgtctt gccgatactt ggaagaatgt tggaacaaaa aatgatctat
    29041 cggagatatc aagccatcgt atggggagaa attgccaaag atatgaccat caccaataaa
    29101 attggacgag atcgccatga tcggagaaaa agggtcgtag atgataaaaa tggtcagagc
    29161 gccttgacac atgtcactgt caacaaagta ctttctgaac aaacacaagt ctattgtgtt
    29221 ttggaaaccg gacgtacaca ccagatcaga gttcatttag ctcataaggg acatccgatc
    29281 gttggagacc ctctctacca aaatagaaca gctgaacgac tgatgctcca tgcattagaa
    29341 ctgcacatga tccatccatt tactcaagaa aaaatagtcg gtaaagcttt acctggatta
    29401 tgggaataga aataaaaaag aacagagtct tatcagcaga taatgataag actctgttct
    29461 ttttgtgtaa tttgtctgct tacttttgat gttcgtgttc tgctaacagc aaactaccga
    29521 taatcccact ttcattcggc agtccccaac gaacgatata ttcacttact tgaggtgttt
    29581 ccatataacc acccatcaat tcaacaaatt gctgacggat tttttgaagg agatgatcct
    29641 gattcattac cccaccgcct aaaataatct tttcaggtgc taacgtcaac gtatagttga
    29701 tcagtgcttg agcaatataa taagcctgaa tagcccaaac aggatgatct tgcggcagat
    29761 cttgcccttt gatccctgtg cgtgcctcaa tagacggtcc tgctgctaat ccttctaaac
    29821 aatctttatg ataaggacaa gtaccttcat aatggtcatc tggatggcgt ttcacccaaa
    29881 tatgtcccat ttcaggatgt gcgatccctg aaaaaatttc ttcattgatc acgacacctg
    29941 caccgattcc tgttcccacg gtaaggtaaa tacaactatt ttttccttgt gctgctcctt
    30001 gcttcaattc cccatatgca gcagcattga catccgttgt ccaagcagca ggaatattgt
    30061 agcgttcctt gattgtgcca agaaaatcga attgtgccca cccttttttg ggagttgcca
    30121 aaacgtatcc gtaattatct tttgttttgt cgataccaat cgggccgaat gagccaatcc
    30181 cgattccctt caattcataa cgatcaaaaa attcaaatac ctgatccaat gtttcttctg
    30241 gtgttgtggt agggatactt aatttttcta aaatttctcc accatctgaa accgcacaca
    30301 caaattttgt tccgcctgct tctattccgc catacattcg ttttcctcct cgaatccgat
    30361 tgtccgaatc tcataaggtt ggattattgg gacaaccgct tgcttcgttg gttcttcaag
    30421 cagattcaat atgacagttt ttccattgtc tttagttatt tccaattttt ctaaatgact
    30481 agacatattg aaacctctta ctactacttt atcactaaac ttgctgcgtt tcaaagctgt
    30541 aattgcaaac gtttcacttt ttatcgttaa atattgttgt tttgaaatca atgttccttc
    30601 atgatgtttg atttgttgag tggaaaaagg aatctgtgct gcgtatgcat gcaaataagt
    30661 agagaatttt tcttctggtc catgtagttc aatgctgtaa ttgaaccgat gctcacctag
    30721 acactgtgct tctggtgttg gaaaatatcc ccaatcgcct agttctccca cactgcgaag
    30781 tagcgtcact gcgatttgtc cgtcttctgt tacttcatat tcattcaaac caaagtttcc
    30841 gactgttaca ccgtactctt catcatgaat attgacaaat gcttgctgat gttgcggatt
    30901 cgtggggttt tcccaacttt tagacacatg gtttgggcgc ttcaccactt caaaaatact
    30961 atctgcttcg tgatgttcca catgtaactt tgtaggaaac aagacacgta agcgatgatc
    31021 tttcatctga ttgtcgagag tcgtttcaaa gtcgattttt ttactatctt ttctgattgt
    31081 catgattgtt tttatttgaa ggattcgttt ttctttggat cgttctgctt ttcgataacg
    31141 aaattccaca accatctgct gttctttttc taaacgctcg tctgcagaaa cagggatttc
    31201 catttcctgt agaatactta tttttgctat ttccggtgta tctactaata cttttacttc
    31261 agagttttct ttgttactgg ataggattgg ctgatcacag aaaggttgtt taaagatata
    31321 ctcatttcct atatcgccga tatcttcaaa aatatgcaga ttattcaact ttcgtttgtt
    31381 tttgcgatct tccattgtaa tggtaccatt tttttcaatc gtcaaatgta gagggccgtt
    31441 ttcgatgatt cgtccactct gatgaatcat tgtttccttt tcttctgctt tcgcttcccc
    31501 ttctacaagc gcgaatgttt cccaagaaaa tgctggcatg ttttctaatg gtaaagtcac
    31561 tttgatcatt cttgccatat atggcacacg gaaacggtct ttcggcaaat cataaccaaa
    31621 ttggacagtt tcttcagata aaatagctgg tatagtggcc ccactttttg tttctacatg
    31681 atacttacgt ttaggcaaat tttctaactc ttgatatagt tgttcaggta ttccttcagc
    31741 gaatcttttt ctttctaatt cgatggttat ttctgcttca ccagttttat ccattcctgc
    31801 agtatttaca atgacaaatg gaaagctgtc ttttgggaat ccggtcgtat cgattgctgc
    31861 ggtcagctgt tctaaggaat cttgtgcaag atactttcca acttcatctg ctttttcaaa
    31921 acgaggtatc atttcccgat ggacagaatc gactgaacaa ccgcaaatac tgtcgtgagg
    31981 atgattttgc attaatgtct tccaagcata atctaattga tcatggggat agtttccact
    32041 aacttcataa gccatcgtcg caagtggttc tgtaatattt tctagctgtc gctggacctt
    32101 ggtattccat tgttttaagt aaacccgagc agatgctgta ttggctaaag tataccagcc
    32161 atccgtttct tgacttgtca attctccttc tactgagcct aagtcttctg gcacatcttt
    32221 ttgaactgct tccagatagt cagtaaaatt agaatgaata aactcgtaat ctggaaacag
    32281 ttcatttgcg agatggattg cttttgatat atctttttgt accggttgat gatcaacacc
    32341 attcatcatc aataagtggt tcgtagaggc atattgttct gcatccgcta atttttgctt
    32401 ccaaaaggca agagctgctt ctttttctac tggaatctca ttcccgttgc tataccaatt
    32461 ggcaaatagc agaccaaaga ttgctgtttg atcaggtcct ttccaccaca tctctgagta
    32521 ttgggaagaa tagttttctg cttccaaaac ttgattatca aagccaattg gttttacacc
    32581 acggccaaac gctgctgctg aaataccagc ttgtttcatt aattgaggcg tctgtcccat
    32641 atttccaaat gtatcgggaa aatagcctaa catgacaggg gttccccatt tacggctttc
    32701 ttccatgcca atcagcatat ttctcacgtt cgattccgag ctgataagaa aatcatcttg
    32761 caagatataa aatggtccaa ttctcaattt tccttcattg attgcttgtt ggattgcctg
    32821 tcttttttct ggccttactt gaagatagtc atctaaaata atcgtttgcc catccaaatg
    32881 aaaactgttg aaggacggat caacttcaaa tagctctagt agatcgtcta tcaattcaat
    32941 cagtcgcatg tgatgctgtt cgtaagccat gtaccattca cgatcccagt ggctatgaga
    33001 aatgatatat actttctttt tcatttacga tacctcctac ttttctactc taatatcaaa
    33061 gtaatccatg actaattcgc aaaacatcat atttgcccaa gaaaaccatt ctctcgtata
    33121 attttcagga ttgttcacat cgaatccttc gtgcataagg tgtgttcctg cgtcagttgc
    33181 tacaagtaaa ttcaatatcc gctctttttc agatttgtct gacgttgtca ttccttccat
    33241 agctaatgca atcggccaaa cgtatttttc tggcgtatgg gaggatccaa ttccttttgc
    33301 gtactttcct tcatagaaat atggattttc tttactcaac aatgttcttc ttgtttgcag
    33361 ataatcttcc tcttgttcag aacaatatcc caagtaaggt gcggcgatca gattgggcac
    33421 attactgtca tccatgactg tggcgtttcc cattccatct acttcatatg cataaatcgt
    33481 ttgccctttt tgattaaccg ttcgtccgtg attctgaatc ccctcttgga tctctttctt
    33541 caatttctgt gtttgctgta caatttcttt atcatcgagt atcgtttgaa aaatttcttc
    33601 cacataacct aacacgacca ccgcaaacat attggatggt accagatatc cataacggca
    33661 agtatcatcg ctaggacgga aaccagacca tgtcattcct gtaaaagctg tttttgcacc
    33721 tcgcccgtca ttgatcagcg tatcttcttt tcttgtcgtg tctcgtacaa atgtataagg
    33781 agagtgttca tgatcttgct ctgttttgaa cacgtccaag atttttttga taccttcaat
    33841 aaaagattga ttgaattgat cagtttttcc tgtatttttg taaagaagat aggccaactg
    33901 tattgggtag cataaagaat caatttcata ttttctttcc cagatccaat cgttcatttt
    33961 tgtatgatct gtttggtggc cagcaccgtt agcttcttcg ttgaaggcat tcgcatatgg
    34021 atcgatattg atataaaaaa attgcttttt gactaacccg ctgatcatgt cagctaattc
    34081 ctcatcttct tttgcaatca ctaaataagg acgaacttgt gcagtagagt ctcttaacca
    34141 catcgctgga atgtctcccg tcaataaaaa ggtggttccg tcattttgct ttttgactgt
    34201 tgtcaacaga gtattggcaa atgctgctgt aaaattcttt gcccagtccg catggatatc
    34261 cccacatttt tgtgtgatat cttccataaa tttctgcacc gattttggta tatctttata
    34321 agccactaat aaaaccttct ttctatttga tatatcttta aagattatac ttcatacagc
    34381 caattatgtc tacgctttca aaaaatatct ttttatttac tttttcatcc attatggtat
    34441 tatttgaata tgatatatca ttaagaggtg tttatatgaa tcaaccattg taccagacaa
    34501 tatttaatga tttgcaaacg gctattttaa ctggtgaatt acctgtagac agccaactac
    34561 caactgaaaa agaactttca cgtacctata aagtaagcag aatcacttcc aaacgtgcat
    34621 tgactgaatt agagcaatta ggacttattt atcgcgttcg cgggaaaggc agtttcgtta
    34681 aatccgcttc tccggaacag attcttgctt ctcctacaaa aacacgacgg attctgtttc
    34741 ttcttccata tgtcgctgat ttgtctgttg gtgattttag caagggattg aaccctgtga
    34801 tgcaagataa acagattgat gtcatgatga cgacgcttga ctttctacaa aataaaacag
    34861 caaaagagct aatcaatgaa tttgacggat tgatttatta tgctcttgat acagaacaac
    34921 atcttgactt actttttgag ctttcattga aagaattccc tgtcgtcata ttagataaaa
    34981 aaatctatga gttacctttt cctaccgttc tttccgataa tttccaaggc ggtgcgcttg
    35041 ctgcctctta tttgatcgaa cagggacatt caaaaatcgc ttatttgttt ggtacgcaga
    35101 ctcatccaca atccgttcga cagcgttacc ttggttacct tgaagcattg gacaaagctg
    35161 gtttgacttt ccacacttca ctggatgaca aaagcgcgac attagatgaa ttgctttctt
    35221 atatcactaa gtatgaagta actgcttttg tttgtgaaaa cgatgttgtc gctatccaga
    35281 ccatgaatct cttgagacag catggttatc atatccctga agatttttca attatcggat
    35341 ttgatgatat ccaagcagca gctcttgttg acccgccact gacaaccatc gcacaagatt
    35401 ttgaacagtt aggtaagctt gcgggagatg ctttggtcac ttggttagag acaaaagatc
    35461 gaccatccga tagtaaattt ccagtttcat tcatcaaaag acaatcaaca aaggagaaat
    35521 aaaatgaata tcggtcaaat agatactaga catgcaacag cgaatcagcc aaatttctct
    35581 aatgggaact gccagcctta tactggcgtt ccttttggga tgaattattt tgcaccgcaa
    35641 actacggatc aaaacggcag ctggtggttt catcctgatg accgcgtttt tcaaggctac
    35701 cgtcttaccc atcaaccaag tccttggatg ggtgatttca gtcatatgct gtttacgccg
    35761 atcaatggaa aactacagga gaacaccctt ttccatgctc aaagttctta ccgcccagaa
    35821 gaaagtatat tttgcccgac tcatctgtcg atctgtcaat tacgttatgg catctgttct
    35881 acattgatac ctagcatgta tggcggaatc ttatctattg attacagcaa aaatgacagc
    35941 ggtttacttt tatcctttcc gggacgccat cagctctttg ttatagatcc ttacacagtg
    36001 gaaggaaaaa tcacacattt tgctggttca gaagatcctg atttttcttt ttattttata
    36061 ttgagatttg aatatccttt aacaattgat gagttagctg tttccaacaa aaatagcgac
    36121 tcgatcccgt tgtattttga acagacaaaa aaacaaacta tccgatttgg gacatctttt
    36181 atcagctcgg aacaagcagc ttataacctg gaactagaac aaaaccaaag caaagagact
    36241 taccttgctc acagtcaaga acaatggcag tcctattttg atcgtataca gctcacacac
    36301 catgatcaac aacaattgcg gacgttttat cataatttat atcgggtgtt tttatttcca
    36361 caaacatttt atgaaattga tcctagtggg caaaaaatcc actatgatac gactagtcga
    36421 tctataaaac ctggtgttct ctatacaaat aatgggtttt gggatacgta taaaacggtt
    36481 tatcctcttt attctttgat tgctgtagaa aaatacgaag aaatgctgga aggttttttg
    36541 aacagctata gagaaagtgg ctatttgcca aaatggctgt ctcctgatga acgtggactt
    36601 atgcctggga cattgatcga tgcggtcatt gcagatgctg ctgtaaaaga tatccgacga
    36661 gacttgatgc ctgaatttct aacggcaatg gaaaaaggtg ctacggttca aagcaaacat
    36721 ccaaattatg gacgccaagg aacagaagat tacttgacat atggctatgt tcctaatcac
    36781 taccacgaat cagtcaatca cacattagac tatgcttata gcgatttttg tattagccaa
    36841 gttgccaagg tactagaaga agaggaaatg acgaagaaat atcagcagca atcattgaac
    36901 tatcaaaata tctttgacta tgagtcagga tttatgcgtt caaaagataa agatggcaag
    36961 ttcagagaac cattttcagc taatcgttgg ggacaagatt atgcagaagg aagtgcttgg
    37021 caaagcagct ttgctgttta tcacgatttt tcaggactga tcggaaaata tggtggcagt
    37081 gagagatata aagaaaaact cattgagctg tgcaatcagc ccccaacgtt tgacccagga
    37141 ggatatggtt ttgagatcca cgaaatgagt gagatggccg cgatagaatt tggacaatta
    37201 gctatttcaa accagccaag ttttcactat ccttacttgt tcagttatat aggacaacct
    37261 gaaatggcac agccactgct taaacagttg atgactcaga ccttcgacga ttctccaatg
    37321 ggttaccctg gagatgagga caatggaagt atggccgctt ggtatatctt caatagtctg
    37381 ggattttatc ctgtcacgcc tggaaccggt gaatatgtta tcggtatgcc attaattgac
    37441 aaggctgttt tgcttttgtc aaatggaaag gaattgacga ttgaagcaac gcctaatcgg
    37501 ccgcagcaac aatttatcca taaaatcaaa cggatggaca aagaatatac gaaattatat
    37561 tttacccatc aagatttgct gaaaggcggc acgatctctt accaattagg gatcatccct
    37621 caccctaaga aatatcaagc aaaagatttg cctttttcct tatcaaacta taattaacaa
    37681 gaggttgtga aaaagctgtc ctgcatcaag taataagaac agccaagcaa aaatagatcc
    37741 tcatattttt cgtttggttg gtcttattac cgcagatgca agcttttgaa caccgtttat
    37801 tgagaggttg tgaaatcagc gttctgacct gagtattaga gagacaaatg gcaaaatagt
    37861 tcctcatatt ttgccatttt ttgagctaat accgagggtc agctgattga acaccgttta
    37921 gtggagaggt cgtgaaaaag ctgtttagtt tcgagtatta agaaaaattt tgtgaaaatg
    37981 gctttccaca tttttgcata aatttggctt aataccgaga aactggcttt tgaactccgt
    38041 ttatcgagag gtcatgaaaa agctgccttg cctcaagtaa taagaacagc ccaaaattag
    38101 atttctagac aattttttat aagttatttc attacattgg ctttttcttt tatgattctc
    38161 cactgttttc tcggtactct ttaggggatc tgccgacgat tttcttgaat aatttagaga
    38221 aatacgtggt gttgttgtag ccaatctcac ttgctatctc attgatgttt tgattactgt
    38281 gcaaaagcag ttgtttcgct ttttccatac gtaaatggtt caaataagct gaaaaacttt
    38341 ttttcgtttc ttttttgaac agctgtccta gatacatgac atttaaatgc aggttttctg
    38401 acacttcttt gagtgtaagt ggttcttgat aatgctggtg caggattacg attacttttt
    38461 ccacattgtc actgtacttt ttctgtctct gatgcttttt gatgaatgac agaagtagct
    38521 gctgaagttc ttgcaccgtt tttgcctgat ggattttttc gattccttgt aaatattcct
    38581 catcttccag atcaatcaat tcccgttgaa tctccataaa cagtaaaaaa ctggaatatc
    38641 gtatatcttc cggcatcatg gctgctaact gaaactgttc aaaaaaatca gtgatcattt
    38701 tttgagcgac ttccaattgt ttattttgca gcacgcgtct gaaacttgaa aaatcgatgg
    38761 tttgatcttt tgtttctgct tgtactgcgt aaaaaacatg ctcgtcttta tctccataaa
    38821 attgatgcaa ctgtagattg tctttcgctt gttggaatgt ttcagggatt ttgtccaaag
    38881 aatctgtctc ttccccgata ctgatcagcc attcctgatc aggaaagttt tcttgaacaa
    38941 aacaacagaa gccatcaact tcatctgcct ccaacaacaa cgtgatcaat acacggtctc
    39001 cgtaatttcg ttgatagtaa aagatttgtt gccgttcttg caaccatcta ttgataagag
    39061 tatcatgagt tcttgccagt tgaatcaata ctatcctatg ctgcttctca cttatttgtc
    39121 ctattatttc ttcttcactt atttcatcca attcttcatt caaccattga ctgaataaca
    39181 gttcttgata gatttcttgt tgattttttt gttcattttg ctgatctaac cgtgttttga
    39241 ttttcttgag gctttcaacc aattcgaact tattcacggg tttcatcaaa taattcacgg
    39301 cgcctaattg catacctcct ttcaaataat cgaattcttg gtatcctgat aaaatcataa
    39361 attcaaaatt atgacgttct ttttgagccg cttcaataaa ttccagtcca tttatttctg
    39421 gcattgtcac atccgttact actaaatcaa tctgtcgctg ttcaagtaca gctagtcctt
    39481 ctttggcatt ctctgctgtt gctactattt gaaagcctaa actttgccaa tcaattatct
    39541 tttttaaccc attcaaaatc atgtattcat catctaccaa taacacttga tacacgctca
    39601 tccctccttg aatgtaatag aaacggtcag tccacctgtc tcattattct caagcgacat
    39661 ataataactg ctgccgaagc tggcacgtaa acgttcattc acgttttgca gaccgataga
    39721 accgtgaagt tccactttag gatgagacag tttttcttca atctgtttta gccgtttttc
    39781 agtcatccct tttccattat ctttgatgat gatccttacc cgtttgcctt cctgcaagac
    39841 tttgacactt agcgcattat cgaatcgagt aaaatcaata ccgtgtttaa aataattttc
    39901 aactaatggc tggatcacaa atttaggtac ttcgatcttt tccaaatcag gatcgatcat
    39961 aaaatgatag gctactcgat taggataacg catttgatac aaatagacat atttttcgca
    40021 aaagtccagt tcttctttta aagtgatcgt tttctcttga ttcgtattgt ttcgaagcaa
    40081 agcggaaaac gcatacacaa catctgctaa ttcttcactt ccttcgctga ttgcatacat
    40141 tcggatatat tctaatgtat tatataaaaa gtgtggattg atctgtgctt gaagcgcccg
    40201 catatgtgcg tcttgctgtt tgatttctaa tttgtagatg tcttctacaa actgattgat
    40261 gctatccaac atttcgttga taccgattga aagttccttc agttcatatt ccgtgtttgt
    40321 tatgtcgatc cgcgtctcta aattccctgc tgctgtttga tccatggcct gcatgacgat
    40381 agatacttgc tgtgaatatc gtttgaatgt tttataaaga aagataagca gtatattgtc
    40441 taaaataatg ataccaatga tcagcggggc aagatttttt cccgtttcga tttttacact
    40501 ttttttatct agtacagcta atatacgata atcatcagac agttcttggt gttgaacaaa
    40561 attgtttttt tccagatcgt caatcggcaa ttgtgaattt ttcttcagac tctctgtgat
    40621 gatcatttct tgtctttcaa acgtttcttt agctatttta ttatcatgga atgtgtatag
    40681 tcgatttgtc gttccagaaa tcatgtataa agagagtcca ttgaatgacg ttagattttc
    40741 taaaatcata tctagttctt tcttttgaaa accaataaaa atacttcctg actcagctcc
    40801 tccttgtgtg ataggcaact tgatataaaa ggcgtctagt aattttggtg tccccttttt
    40861 tatttcccca cctttgtttt ctctagaaga ttcataatac tccggtagtt gattcaacac
    40921 gatatatatc gcacttagtt cttcataatt tgcgtagaga tctttgattt gatttgggaa
    40981 agaaactaat tcatcggtac tgttttgctg ttcgtataca tacatcagat actgatctat
    41041 gggttttgtt aagtaggtcg tcacattttc gatcttttcg gaattcccta caagctggtt
    41101 tttgatgatc ctactcaagg ttgttttatc attcagcatt ctgcttgtgc tttgaacggc
    41161 atcacttgtc atgttttctg cctgtttttg attttgttcc caagtatgga tactaaaaat
    41221 aacggtagca gtcatcgtta caccgatcat gataaccgcg tatttccgca acagacgatt
    41281 tagtaaggtt ccttttgaaa atagaatccg aaacttattc ataactttcc aactgtttat
    41341 ctaattcttc acgccaatgt gttgtcatcg taccattcca aaccgtcaaa agaccaatga
    41401 caccaaacag gatcaatcct ttgaattgcc aagtcacgcc taaaattata ccgctaccaa
    41461 taattatttt tagaaaagac gaaaagctgg aaaaaaccga aataaaactt agctttagca
    41521 gattaggaac agagatcgta tattcactat ctaaaatcat actgtattga taagtagcaa
    41581 ataggcagag caaggcaaaa agtaaaataa aatcaatagc taaaaaaatg agccctttga
    41641 tttgaacaga taaatataaa ttgtaactca ataaagcagt accagataaa aataaactaa
    41701 atagcagatt gcctcttaaa aaatttcttt tatatatatt ccagcctctt tttattgtga
    41761 tttctttata ctcgaatccg tacaaataaa atagttcatt gactgttttc catgccgcac
    41821 ctaatccaaa gatgatcccg ccggcaaatg tatatatcca gaaatacaag gttagtttta
    41881 taatgaccca gatccggata aataactctt ccaaaacctt ccctaacata tcttctcctc
    41941 caaacattaa gtttatactt tctcattata caaaaaaagg ttgggcaatc agcaccttga
    42001 cctgattatt agagaaacaa acggaaaaat agttcctcat attttgtcgt tttgtgagcg
    42061 aataccgagg gttagctgat tgaacaccgt ttaccaagag tttgtgaaat agccgcttag
    42121 tttcgagtag taagaaaaat tttgtgaata tggctttcca catttttaca tatatttagc
    42181 ttaataccga gaaactggct tttgaacacc gtttattcgg aacaagaggt tgtgacacgt
    42241 cttttgtcac aacccctttg tctttattca ttattattct tgtcggaact catcatattg
    42301 tttttgcatt tctttcaata ccttatcata gccagccttg tccaaagcgt ctttcaattt
    42361 cggtaatgtt tcatctggat caacagtccc tgtattcaat ccatctaaat attgattcat
    42421 gacattagaa atattactta attctgtttt gacactgtca gtgttgaagc tgaatcctaa
    42481 aatcggtgaa gtttctgcat cagcaatcga ttgatcacgt ttggcaatca tgtcatccgt
    42541 aatagactct tgtgtatata gaatcttgtt gtttcctgta ttccaagcag acatatgtgt
    42601 atttggctga taaccatcta gcaatttgat tttatcatct gaaccatcga ctttttccca
    42661 tgcttctccc tcgatccccc aaacaagccc attcaataat tctggatcac tgttaagcag
    42721 ccctagaagt tctactgatt tttctttatt ttttgaagta tttgatacga caaaatttgc
    42781 catttgtgct tgagctgtag attttagtgg aacagtaatt gcttttgaga caagttcttg
    42841 ttttgctgca tttgtcaaaa ttgtatcgcc ataatcatac ggtccttgtg tttctccacg
    42901 cattagccaa gtatttcctt ctagtggata atccttattg ctcgttgcag cgtcacttgg
    42961 aatgagtcct tgtttgtacc agctatgcat tgtacgtaac agtccgatat acgtttcatt
    43021 atcgtattgg ttgatgattt tcgaatcatc tccatccaga tcaatagcaa atggtaacgt
    43081 attccctaaa ggataatcaa agttcccttg gactttgaag ccttgtccaa tggcaaaagc
    43141 tgccgtgttt ggttcttttt catggaattc cttcaagact ttttctgcat cagcgtaaga
    43201 ttggattcca tcaattgaaa gatcgtattt atctagtaaa gctttgttaa aagtaagtac
    43261 ttgttgagca aagacatttc cattgactgg gaaagcatat aattttccat tgaccagatt
    43321 tcctttaata tatgcttcat ctaaatcatt ataagctttt tcagcgtact taggcgctaa
    43381 ttcagtcaaa tcagcaaagg ctcctttttg ggcattggaa acatagttgt tcgcaaaggc
    43441 gatatcataa ttttccccgg aagaaatgat gacgttcatt ttcttttcgt aatcgcccca
    43501 gccaatgtaa ttgatattga cttttacacc gatctctttt tcgattcgtt tgtttgctac
    43561 ttccatcaat tgatcgaaat tatctggttt atctccgatt tgatacatct gcaaagtgtc
    43621 cgaattgcta gcttctgcct ggtctttaga accagaacct ccgcatgctg ctaatgttcc
    43681 caacaacaac gctgaaacac ctgtaaaggc tagtttcttc cataacttca tttttcttcc
    43741 tcctaatttt atgatttata tttttgtttc tttattcttt tactccgccg attgttagac
    43801 ctttgacaaa atgcttctgg aaaaatggat agctgatcgc aataggcaac gtcgagatga
    43861 ctacgatcgc cattctggca gattctcctg gaactgaggc taatccgcct gtcaattggc
    43921 ttcctgctcc tgaattttgt gtcagatatt gaatattact ttggattttc atcaaaagat
    43981 actgtagtgg aactagctta tcatcttgga tgtatagtaa agcattgaac cagtcattcc
    44041 agtaacccaa tgcggcaaat aaactgattg tcgcaatacc tggaacagct aacggtaaaa
    44101 cgatctgaac aaatattcgc agttcgctag ctccatcgat ccgtgccgat tcaataatcg
    44161 aatcagagac cgaacgttta aagaatgtgc gcatcacaat aatattgaag ggactgacag
    44221 ccattggcaa gatcaatgcc caaatcgtat cttttaactg aagaagattt gtcataacaa
    44281 gataattggc caccatccct ggtgagaaca acatcgtaat caaacaaaaa acggtaaaaa
    44341 aacgacggaa tggaaagttt gaccgtgaaa tcgcataagc atataaagaa gtagccgtgc
    44401 tattgatcag tgtacctaaa acagtaacaa gtacagtgac gaaaagcgct tggaaaattt
    44461 tatcctgcat ctggtcgaac aagtacgtat aaccaaatat actgaacttt tctggccaaa
    44521 aactgtaccc gtgagtcgac aatatggact catcggtaaa agagatggcg ataacaaata
    44581 taaatggcaa aatacaggaa atagcaaaga gtgcaatcat tatattagag aaaaaattag
    44641 ccgtcttatt gaaagagcgg acttggactc tctccacttt cttttttttc atttttttcc
    44701 tccttagaat aatgcagaat cgctatcaaa acgtcgggcg atcgtatttg tgatcatcaa
    44761 caagacaaag ccgacaactg attggtaaag tcctgcagca gccgtcatac caaaatctcc
    44821 tgtggatgtc aggccattgt agatatacgt atccaaaact tgagtcacat catataatgc
    44881 accggaattc ctaggaatgt tataaaacag gccaaaatca gctcggaaaa tgtttcctac
    44941 ggctaaaatc gttaaaatcg tcattagtgg aataagttgt ggtatcgtca catgtttgat
    45001 ctgctgccat ttgctagctc catcaaccat agcagcttca taataagtcg gatcgattcc
    45061 cataacagat gcaaagtaaa taatgctgtt ataaccaatc cctttccatg taccaatgaa
    45121 caccaagata aacggccaat atttcggttc attataccaa ttgacagctt ctcctccatg
    45181 tccagtgatc cactgattga atattccttt gtcaggactt aaaaaagcgt aaacaaagta
    45241 actgataatg acccaagata gaaaataagg tagtaaggac atcgtttgat aaactttaac
    45301 gagtcgttta tttctcaact cgctcataat gattgcaaat acgactgaga tcagcagatt
    45361 caaaaggata aatgctacat tatataaaat tgtattttta gtgatgagaa atgcttgatc
    45421 cgaagaaaat aaaaatttga aattttccca acccacccaa ggactttccc tcaagctagc
    45481 caagaaccca tctgctgaaa tatgaaaatt tttgaatgct acgacattcg ccattacagg
    45541 tatgtaaaag aaaaaaatca tccaaagcat acccggtaca gccatcagga caagtgcttt
    45601 ataacgccat agattcataa aaaaatgatt gaaccccttt cgttttttca tctaaaaacc
    45661 cctttcgatt cattcgcaac gtaattatag cgctttcaat aatgagaata aataaacaaa
    45721 gtatagggaa aatgaaacaa agtataggag aggttgtgaa atcagcgttt tggcctgagt
    45781 attagagaaa aaaacgggaa aatagttcct catattttgc cgttttttga gctaataccg
    45841 agggtcagct gattgaacac cgtttatcag aggttgtgaa ttcagcgttt tggcctgagt
    45901 attagggaga tcgtgtcctt actatctaac tctgagtcac aaggaacaat ggggactgta
    45961 acaaaagtct tgtcacagtc ccttattccg aacaaacggt gttccagaag tagcttctac
    46021 ggaaataagc cgaaatttca caaaaaagcc atccgaaagt cagacggaca agtctgcttt
    46081 tcagatggcg ctacaactac tctgcttatt cttttagcta tccattcaat tatttttctc
    46141 tcacttgttc tagctcttct ttggtcatgg gtcgatattg ccctttattc aaatctttag
    46201 gcagccaaaa ttttcccata cggattcttt ttaaatataa gacttctttt cctactgctt
    46261 tgaccattcg cttaacttgg tgaaactttc cttcatgcaa aatcagacgg ataattgaag
    46321 tttctgtctc ttgatctact tgatcaattt ggagttttgc cggcaatgtc tgctcatcgc
    46381 catcaataat cagtccttct gcaaataaac ggatatcttc ttctgtcatt ataccgctaa
    46441 cctcagctag atattctttc tccacatgtt ttttaggtga tagtaactga tgggcaaaca
    46501 ccccgtcatt cgttaaaatc aagaggccct ctgtgtcttt atctaatcgg ccgactggaa
    46561 aaagatcttc ccgataatcc tggtcttcca ataaatcgat tacggtttga tcatgattat
    46621 cctgagtagc agaaatgact cctgcaggtt tatgcaaaat gtagtaaaaa tctttttgat
    46681 acgtaatgat ttctcctaaa taagtaaccg catcttcgaa ttcatttact tgcgctttat
    46741 cgcttttcac cacttgtcca ttgacttcga tcgttccttt tttgattaat cctttgactt
    46801 ctttgcggct gccaagtccc atatctgcta aaaacttatc taaacgcatg cgtttccccc
    46861 atttcaaaag agcatcaatc tagctcttct gattcactta ttttattttt aatttatgtc
    46921 gtaaacgcgc tattcctggt cctagtagtc tttctgccaa gcggattttc aaagctaaat
    46981 aaccatacac taaagcccct atacctgcta ctacaagaat caacaataga gactggaatt
    47041 tgctatcttc gtttaaaaaa gcaccaaaaa tcatctttgt gatccacgaa gccaaaagca
    47101 taatcaaggt aagaataaag atcagcaatg tcctgcgcca gacaaaactt ggtttaaagc
    47161 gggctacttc atggattctc tttaagatca aggtacaaga cacagcaaat ccgatcattg
    47221 tcgccaataa agggccgtat acttcaaata aacgaatggc tgggtattga agaaggagtt
    47281 tcactagaaa cccaataacc aaataattaa ttgccgcctt gttttcaaac attccttgca
    47341 gcatgttgga aactagcata tatagtccta ggaagagtcc tacaaaactt gcttggatca
    47401 gtacattact tcctagagaa tccggtgtat aaaataatgt atttagcgga taggcaagta
    47461 acataacacc aaatgtcgcc ggaaacataa aaaatgaaaa caactgtaaa tttccgcttg
    47521 tcaattttgc cagacctact cgatctttca acgtaacagc ttctgtaatc aacggtaacc
    47581 cagtagacgc gatcgatgtt gccaatgcaa taactaccat cgtcaactta tctggattgg
    47641 cactaaaaat cgaaaaaaga tccagtaact gtgcattgga atactcggtg gatgcagaca
    47701 tgatcttcat aaaagtgaac tgatcgacta atttgaagat cgttacccct gaacccacaa
    47761 taataaacgg aatcgcttct ttaacggtat ctaacaacaa ttccttcgtt gcaatattta
    47821 ttttgttttc gctgtagtga ataaacgccg atgtatacgc ttgatgtttc ttcaaaaaat
    47881 acaaaaggac aagaatactt gctagcatac cgataaatgc cgcgaaagtt gattgcgtga
    47941 ccgccgtcac atagtcacca tccatgactt tcatgatgat gaatgtagcc aatagcatat
    48001 aaaaaatacg tgccacctgt tctacaatct gcgacagcgc ataaggcatc atttcttgat
    48061 tgccttgcaa ataccctcgt atcacactca tacaaggaaa aaccaataca gcgatactca
    48121 aagaacgcat cgttggaatc agttcttctc cgccaccaga agcatgagct agccaaggag
    48181 aggcgatgta catgaacaaa gcaaacaaca ggcctaaccc gcccatcatc tgtaatgcac
    48241 ggataaataa tcgtcgtgaa gtgccatatt cattcaatga attatagcgt gcagtttgtt
    48301 ttgcaatcgc agcaggtatt cctgctgtag agatcagtag aaacaatgca tatatattgt
    48361 atcccatatt gaataatcca ttggcagcct tagcattttc gcccatccaa gcataccagg
    48421 gaatgatata gacagcgcct agcaatcttg aaatgatatt actggcggtc atccaagcag
    48481 agcctcgtgc cattttttct tgcggtgtta attgatctaa tgatggtttt ctttgattat
    48541 ccatcggtca gattccaact ttctacaaaa cattactatc attttaattg gtaaaacaga
    48601 aactggcaat cgattttata taacttttat aagaacaatt gtttttttga attcggtttc
    48661 gtttttttgg taaagtaaag aaatatacag ccttttatgg ctaacattta taggagatat
    48721 caatgacaca agaaataaaa aaactacccg ttatattttt aggtcttttc tttatcacag
    48781 ttggattgaa ttggttcttg ctccctcacg atatagcttc cgccggagtg ggcgccatcg
    48841 gacatttagt tgaaacggcc ttttctatcc aacgctcagt tactgtttgg agcatcaatc
    48901 ttatgatgct gcttctagcc gctgtcctat taggaaaatc agtatttttt aagactgtag
    48961 cgggaagtct gctttttcct gttgttttag agattgttcc aaaagtggaa atgatctcct
    49021 cacatttctt ttcactgatt tcaggaagtc ttttgttcag tcttggggtt tacacattgt
    49081 acacagtcgg ggcctcaaat ggcggtataa cgataccgcc gattattttc cacaaatttt
    49141 ttcggcttcc aatgccgcaa ggacttttac ttaccaatag tcttatcgtc tttttgaatt
    49201 attatgtttt tggattgttg gagacactat tcgttctcct atctattaca gtcagttcat
    49261 tgtttatgaa aatactcatc cgcttgtccc ctgtgtcaag aaaatttgaa taaagctgga
    49321 ttttgtcata gcggaaacaa acgatatatt aaaaagagtg tgctataata aaccaagaaa
    49381 ttgaataaag cgagggatca atcaatgagc ttctctttag aagacatccg tcatttacta
    49441 ttaaaagaaa atctattaaa agaatttgtt tcaacaagag gctggcatct tgatgtacca
    49501 gaaaaagctg aatttcttca gttaagttat gattcaagaa aagctgatgc ttctactttg
    49561 tttttctgta aaggaatgaa tttcaaagaa gaatatttgg attcagcaat cgaacagggt
    49621 attcaatatt atgtatcaga acaaccatac gaaaattcag cagtaggaat catcgtaacg
    49681 gatatccgta aagcaatggc gcttcttgct atggcttttt acgactatcc gcaaaataaa
    49741 ttgaaagtga tcggattcac tggaacaaaa ggtaaaacaa ctgctgcgta ctttacaaaa
    49801 gcaatcttgg atcacacaac aaataaaaaa acagcattgc tttctactat gaatacaacg
    49861 ttagatggca aaacatattt caaatcccat ttgacaacgc ctgagtcatt agatttatat
    49921 cgtatgatgt cagaagcagt tgaaaatggt atgactcatc tagtgatgga agtttcttca
    49981 caagcttaca aaacacaacg cgtatacggc ttgactttag atgtcggaat cttcttgaat
    50041 atctcgcctg accatatcag tccgatcgaa catccgacat ttgatgacta tttctattgc
    50101 aagcgacaat tgatcttgaa ttcaaaaaca gttgttttaa atcatgaaag tgactatttt
    50161 gatttactta gggaaacagc cgatctattc cagattccaa cgatcactta tggtcgttcg
    50221 gaagaaagca attatcaagt gatccgttca gataaaggta ctcacggttt tactttatct
    50281 tctcatgaag atcgcttagc tatctgcgat actgcttatg atattttgct ggctggagga
    50341 ttcaaccaag aaaatgcagc cagtgcgatc attgccgcag cactaacagg tgcctccagt
    50401 caagatgcac aagacggatt aaaagaagct cgtgtaccag gccgaatgga tcaattgatc
    50461 caatccaatg gagcgcatgt ctatgtggac tatgcacata attatctaag cttgaaaaca
    50521 ttgctgaaat ttgccaaaaa tgaacatcca gatggccgtg taattgttgt attaggcagc
    50581 ccaggaaaca aagcaatctc ccgtcgacat gattttggaa aagtgctatc agaaacagct
    50641 gacatcgcct ttttgacggc agatgaccca gcatttgaag atccgaaaaa aatcgcggaa
    50701 gaaatcaatg aagcaattac taatccagat ttgatcgttc attatgaaat ggatcgtcct
    50761 gaagcgattc gacgcgcttt agcagaaagc gctccgcaag attcagtcgt tattgcggga
    50821 aaaggcgtgg atccctatca aaaaatcaat ggtgtagatg aaccttatga aggggattat
    50881 gcaatcgtga agcggttgat tgaagaataa tggtatttat atactagtgg tttcccattt
    50941 ctaagaaaaa aagggactgt gattttttgt cacagttcct tttttcttag aaatcatttt
    51001 aacttttcac tagctaattc tgctgccttt tctctttctt catttaataa tttattatca
    51061 tagaatttga taaatgggaa ccagataatg aatgcgatga ttgcacaaat gactgctaac
    51121 acagcagctt tccaatcacc accactacca acaaatgctc cgatcccgac tggtgaaggc
    51181 cacggcatct gtgcaagcat cggtttgacg atttccagcc gaatggcaaa ataagcgagt
    51241 gtagcagaag ccatcggagc taagaaaaac ggaaccgcta aatagggatt gtagacgatt
    51301 ggcatcccga aaatgattgg ttcattgata ttgaataatc ctggtacaag tgaggctttt
    51361 cctaacactt tcagctgatc agatttcgcg aggaatgcaa taaagatgat caatcctaaa
    51421 gtcgcacctg atccaccaac ggttacataa gaattgttga attctcctgc caacggataa
    51481 tttgcgcctt gtgcgttagc tgccatatta gctaatacga tcggggtcaa gaaagaagtg
    51541 atgatattcg ctccatggat ccctacgatc cacaatgcat ggatcaagaa ataaatcacc
    51601 atgacaccaa gccatgtatt cgtcaactgc gtaacaaacc caaatgggat agcaatcatt
    51661 ttgaagatat ccgttcctag tgcaactaat aatccattga tgatcatcac cacaaacgca
    51721 atgacaaatg ttgggatcag agcagtgaaa gaacgagaaa ctcctggcgg aacaacatct
    51781 ggcattttta tgacccaatt tctcttgaca cacatacaat acaattcgac cgcaataatc
    51841 gacataataa tagctgtaaa gattcctgaa gttcctaaac gttcgacaaa agagcccatc
    51901 ctaataccat ttactacagt ttctgtctca ttgatagaag caatcaagct gattttacca
    51961 tcagtcatga tcaattcagg gatacacata aagaatgcaa aaacagatag taatgccccc
    52021 gtgagcggat taacatttaa tttttgttca tctcgttcaa ttgacgttaa ttcataagca
    52081 aacacaatgg caaagtaaat agataaaatc cccatcgtca ttgtattgac gatcatgtac
    52141 aagtcactaa ctttgaaaaa agttgcatta aaaaatcctt ctaaaaatgg gaacggtaat
    52201 ggcaaaatat ttaaaaccaa aaacatcgag cctacaatcg tgaaaggaat agaagccatc
    52261 cccgctgcca tgacagcccg aacgatttta agttgcgcaa ttttccccat aggtcccatc
    52321 aggtatttct gcaaaaaatc aaacattagt tattctcctt tcctaaatat aacgcttttt
    52381 catattgttt aatacggata tactgttggt cttggttgag tgaaattttc tttaaacgat
    52441 actgtataaa tgcactggca aaaataccgc ttacaacaag aatgaaaaca aataactgtc
    52501 ctccattgtt atttgccata aaaggataca agaatgtaaa tcctgaggta aacaacactg
    52561 gaatcactaa taaattaaag acactctgga acaaaaaata ccactttgtc catggaacgt
    52621 tcgtttggtg ttttcgataa agctttactt gttctcctac tgcaggcaaa atagctaaaa
    52681 tcagaatcat cggtaaacta cttcctaaac ttttagctga tagaagcaga atcatccatt
    52741 gcatgttcat gaaaaagaaa aaggcagtca ggtaacgtac gattagataa cgattgaaat
    52801 acatgttttt caaactaagt tgcttttttt ctaattcgat tttccttttt actgtatcct
    52861 gcacaatgat ccttcctttc tatttatttt tttgatacaa ataattcatt tcctgtgcaa
    52921 cttccaataa tgtcatcgtc gtcatcagat gatcttgtgc atggaccatg ataatttcca
    52981 tttctatttt tgtcccgctg gcatattctt tcagaagttg agtttgtgac tgatgcgcat
    53041 gaagaatctc ttcatttgct tgtcgcaact tctcttctac ttcttcaaac atctctttac
    53101 gcatcgagcg aaatgcttca tgtatcagtg tccgtgcatt tccgctatgg aaaatgatat
    53161 caaaagaagt cacttgtagt gtttcactgt ttattttttc agccatctgt tttcctcctt
    53221 attcgatcag tttgataaat ttactgctga aattctcaaa aataggttca gctaagattt
    53281 cctgctgtac ttttagctgg tcaactaacc taacgattgc tttagtcacg accgtaattc
    53341 cttcattttc gacataagaa ggcgaaacta aaaaaacaaa atgaatttct tgatattgat
    53401 cccactgcat cccagaagga atcaatgcaa cggctacctt tgtttcgatt ccaactggaa
    53461 cagcgggatg tgggactgcc actgtatcac tgaatatgat ttgtcccata ttttcacgtt
    53521 gctggatctg ctttcgcatt tctcttatat atccttcttc ttcatttatg ctcagctttt
    53581 gcagcaaatc atgaagaatt tcttcctttt tcggttcgta cgtataagtc ttgaaaaatt
    53641 tttcagacaa ctgttcggtg taaatctgct ttttttgctg tagagaaaat gctggtatcg
    53701 gtctatctga ggaatgatag ggtatagatt ctccaactac ctttcgtatt ttacgaacat
    53761 catcttcatt caaaaaaata ctgacatgta aaactggaat tttgaaaaac attgtcgaaa
    53821 gatcaataga agaaataatc aggtcgattc cttttaaagt tgtttcattg atctcataat
    53881 agccttttac acttaccact gtaatatttt taccgaattc actgactacg cgattcttta
    53941 atagttgggc actgccgtaa cccgttgcac agataatcaa tgcatgtagt ttatgcgcag
    54001 ctctttcttt ttccaatgcc gccatgagat gcaaagccaa ataggcccat tcatcttcat
    54061 tgatggtata gtttttcaaa gccggcatgt tgctgaaatg cctttttact gcgtcaaaga
    54121 ctttgggatt ttctttcttt atctcctcag tcaatgggtt ctcaatgcga atacctcggc
    54181 tcaatcgaat catcatcggt gacaaatgat cgagtagtcc attgaaaagc tggagatcct
    54241 ctactaatga aaaatgaaat tcctgagaca atcttttcag aactttcgct aattcctttg
    54301 atagttcctg atcattttga ttttctttgt gattcgactt agccatgaga tgtaatgtca
    54361 aataagcaat ttcttcttcg ggaaacgata cacctgcgat tacctcaata cgttgaacga
    54421 tccgctgcgc cacttgatac tctactcgct tagaaacttc ttgttcgatg cctaattcct
    54481 tgatttcaaa cccttctcgg agccgtttga tccccaaagc caagtgtaaa gtcagattct
    54541 gtatgataaa atccgaaact tttaacttag cttctcttgt ttcatcgaga atgataatga
    54601 ctagctcttc aaaactgatg tctgggaaaa aacggctttg ccctaaatat tctttcaatg
    54661 aatttccata atggtttcca aaaaaagtat ccatgataaa atgccgtcga tttctttctt
    54721 ccccttcgat ccatgtccct tgaccatgct tagaaacgat ttttagtgaa tatggaatca
    54781 atcgctcctt gatttcttgc atgtcctttg tcaaactgga acgactgata taaagctctt
    54841 ctgccaaatc atccataaaa agaatggcat cttccaaaag caattggttc aatatatact
    54901 tttgtcgatc ttcggactca taaaatcctt gcggttctct agcatatcca ggtactattt
    54961 cttgccgact caaaaacaag tcaaatgtca gtctttgtaa aatatgcagt tggtagccat
    55021 aacctggttt agcaatgatc ttacctccgt ttttttctac taactctttg atccgctgca
    55081 agtaattacg gattgtccga tcagataata acaattcaga agctaaacgt tgactggtga
    55141 catactcctt cttgtgatca atcaaatatc tcaacagcaa tttttccttc tgtttcatta
    55201 tcatccactc ctattccatc atatgttctc ttcgatcacc tctgctaact tctggatgcc
    55261 tgtagggatt gggacatacg cttggaaagg gatactaaca acaggctttc caacagattc
    55321 tcctaatttt ttgaatttat ccaaaaacat cgtcgtttgc gggctgatca gaaataaatc
    55381 aaaatcacta ttcttaatca tattatcgcc ttctgtagct gaaactgcat ctacgagtac
    55441 ctcttctctt ttttgcttaa agaaatccgt tgtctttgag gccatcatcg aggatgacat
    55501 tcctgccgca caaataatca atgctttttt cataaatcaa tcactccttt tatttttata
    55561 aaccaagcat atcttgtaag cgttttctat tcaaaatata tatttccgta gcattgcgga
    55621 aaaaatgtgt atcatatctt gaataataat ttctggcacc gtgatatact agtgatagaa
    55681 ttttttcagg aggggattta gtgagtatca cagtgaatat tttgtataca agtacaaatg
    55741 gacaagccag aaaatttgca gaagaaatgg tcgaaagaaa cattgtcaaa gcgattcgtg
    55801 cagaagaagg aaacgagaaa tatgactatt actttccact agaagatcca gaatctcttt
    55861 tactgatcga ccgttggaaa gatgaagaag caatcgaaaa gcatcacaaa tccgaaatga
    55921 tggctcaaat aaacgaactc cgtaaaaagt ataaattaaa aatgaaagtc gaacgtttca
    55981 ctgatttacc gtaaaaagat acacatggaa tgatagcaat ttatcatcca tgtgtatctt
    56041 cactttttcc ttatttttta ttcgatctca tctaatgatt caaaccgtgt ttttctctta
    56101 tttttcggat aagtatttca gtcaccccaa tatacatgat tcctaaacat accccaagga
    56161 tattctcaaa aatgcggatc gttactgctc ctggaacccc gaataaactg gcagtgattg
    56221 ccaaagcacc aaaagaatta aaaactgttt tatatctata ggtagaacaa acacctaaag
    56281 ctagaccacc taatataccg acccagttaa aaggaataaa ttggctgata ccaataaata
    56341 aaacacatcc aatcaatgag ccaatgattc ggtctactgc tcgttctttg aaaccaatcg
    56401 acatcaatcc atagctagat acaatagaag aaaatgcaaa ccctgcccac atcaatcgct
    56461 gaaaagggac atactctcct gcaacgatca aaagactaat gccaaatgca tagatgagca
    56521 tccaaatttt ttcctgcgaa tacatgcctt tcaaaaaaat ctctttaaac aagcttttgt
    56581 ctttgttctt ttctcgatgt tttctgtaaa ggagaactga aaaccaacaa aaaaacaaaa
    56641 ataataagct acttgtttgt gtaaagaaat tcttgttcaa cgaatcttta ggtaacgaat
    56701 aaacaataaa gagatatgaa aagccgtaca aagaagcatt ccccattttg ggatcactag
    56761 cggttgccat taatatgctg cttagtaaaa taaaatgaat caacatgccc ataactgacc
    56821 atttgatcag ctggacatac ggtgctacta atagtgagaa catgacgatt gctaaaccta
    56881 tgatcgaatg ggaaatctta taaccaaagt caacaaaacg aatactcatc aacatacaga
    56941 ataaaaccac agcgtatggt ttatgcttcg attcaaataa tgttgtaatt ataatgataa
    57001 aacaaatggc aaacaatacg atgaataatg atcgtaaaaa caatgaacaa atatacatgc
    57061 acttttcttt tttagaagct gcttctcgta ttttttgttt taaaataaac ggatctagtt
    57121 gtagagactg ataaaaatcc acctgttgtc gtcctttctt tttactttta ttttttacag
    57181 tcatctatga caggttgtaa atactatgga acatcatatg gcgttgtcat tttcttgtca
    57241 actatttttc ctattcatgt ttttataaaa aaacatatcg ttttcttgta tgattaaatc
    57301 accacaaaat aaacaactga gagaacgata tgctttatga atggttctat caaaaaaatt
    57361 ctgagatcaa tagacaaaaa tttgatgatt acagaggtct cttacgaaac cttacagaag
    57421 aaaaaaacat tgatcgttgc tgcagttctc ttccctgctc catgtgtctg tagaaattgt
    57481 ggttctgctg tggtagatgg aaataggaaa ttaatggtgg tgaaaaacaa gaagaaggaa
    57541 accattgtcc actttgaata atataatcat atgccaatgg ttatacgcct aaaaaagcag
    57601 cgctatacct gtaaaaactg tcgaagccat tggaacgctc aaagttattt tatccgtcca
    57661 aggcactcga tttcaaatca tgtcagacat aaaatcactt ctttactgat tgaaaaagtg
    57721 tcgttatttt ttatttcgaa aagttgttag gtatctttga caaccgttat tcgcacattg
    57781 aaagagttga gaagctattt accaaagtct tccaaaagaa ttctgtcttg agtgttgagg
    57841 gttgtgaatt tcgttctcac gtttccgttg aggataaact gagttttatt tccgcggatg
    57901 gcgagacaga aaaattaatc gagaaaattt acctacacaa aaaattcctc gattaacaaa
    57961 ctatttcctt agctgcacta atcctaaaga aatagaattc ttagttacag acatgaacgc
    58021 cacctacttt cagctcacca aacatgttct accaaatgcg aaattggtga ttgatcgatt
    58081 acttggtttt tccacgcctt tcaaggaggc gtaaccttta ttcacgagtt ggttgaagct
    58141 ttccgagaca aaagacccga cttgtttttc tccttattga caaagagcca ttaaaactag
    58201 agaaaaaatt acaactgatc gctgattatt tgtagaaaga aaatcaaagt attacccaat
    58261 aaacttgagt cattaggcaa aaaacactgc gaaactcaag aatgattttc gcagtgtttt
    58321 tgtatatttt ttattaacta gtattactac aaaggtttat ttttcacttc tcaacgcagc
    58381 acaaaattgt ctttacttat ttactggttg ggttcaacct tctctactcc gatactaata
    58441 tttgtttgta taccccttct aaaatcggta gtttccattc aattgatggg tactagttga
    58501 ttttaaaaac tatgcttatt caatttttta ttttagctca tatatttcgt ttctttctaa
    58561 attttatact gatgaaaaca agtagcacac ccaatattga caatataaac tggctaatga
    58621 tggtttctcc agtttttgga agatgcttat ttccactgct tgttggattg tttcctctta
    58681 tatctgagct atcagcaact tttctattct cttttgttaa aaagatcgcc acttcttgtt
    58741 ctttttcgat cttgaaagtt actggtgtcg catccaattc ataccccgtt ggcgcttgag
    58801 tttctaccag ctgatatgct cctggtgcta agccatctat cgccagtttt ccatctgctc
    58861 ctgtggttag acctgactgc agaacggttc cattggcatc ttgtaactca aaaacagctc
    58921 cttgcaggac ttctccgctt tggtcatcga tctttgtcaa cacgacaccg cctggtgtaa
    58981 gtcgattttc tttcgtcaat tctaccaccg ctgtctgact tctttcgatc tcaaactcga
    59041 ttggtgtcgc atccaattca taccccgttg gcgcttgggt ttctaccagc tgatacactc
    59101 ctggtgctaa gccgtcaatc gccagttttc catctgctcc tgtggttaga cctgactgca
    59161 gaacggttcc attggcatct tgtaactcaa aaacagctcc ttgcaggact tccccgcttt
    59221 ggtcatcgat ctttgtcaac acgacaccgc ctggtgtaag tcggtttgct ttcgtcaatt
    59281 ctaccaccgc tgtctgactt ctttcgatct caaactcgat cggtgtcgca tctaattcat
    59341 accccgttgg cgcttgagtt tctaccagct gatatg
//
LOCUS       DACKAC010000011        56388 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.011, whole genome shotgun sequence.
ACCESSION   DACKAC010000011 DACKAC010000000
VERSION     DACKAC010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.011"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            330..1175
                     /locus_tag="I0B46_000684"
     CDS             330..1175
                     /locus_tag="I0B46_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAR2083007.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1456..2676
                     /locus_tag="I0B46_000685"
     CDS             1456..2676
                     /locus_tag="I0B46_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAR2083008.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2673..5393
                     /locus_tag="I0B46_000686"
     CDS             2673..5393
                     /locus_tag="I0B46_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAR2083009.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5377..6321
                     /locus_tag="I0B46_000687"
     CDS             5377..6321
                     /locus_tag="I0B46_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAR2083010.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6390..7400)
                     /locus_tag="I0B46_000688"
     CDS             complement(6390..7400)
                     /locus_tag="I0B46_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAR2083011.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7580..7924)
                     /locus_tag="I0B46_000689"
     CDS             complement(7580..7924)
                     /locus_tag="I0B46_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAR2083012.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7917..8351)
                     /locus_tag="I0B46_000690"
     CDS             complement(7917..8351)
                     /locus_tag="I0B46_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAR2083013.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8489..9226
                     /locus_tag="I0B46_000691"
     CDS             8489..9226
                     /locus_tag="I0B46_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAR2083014.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9219..10430
                     /locus_tag="I0B46_000692"
     CDS             9219..10430
                     /locus_tag="I0B46_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAR2083015.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10637..11416
                     /locus_tag="I0B46_000693"
     CDS             10637..11416
                     /locus_tag="I0B46_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAR2083016.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11486..12148
                     /gene="trmB"
                     /locus_tag="I0B46_000694"
     CDS             11486..12148
                     /gene="trmB"
                     /locus_tag="I0B46_000694"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAR2083017.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12246..14000)
                     /gene="ade"
                     /locus_tag="I0B46_000695"
     CDS             complement(12246..14000)
                     /gene="ade"
                     /locus_tag="I0B46_000695"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAR2083018.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14132..15061
                     /gene="cpaF"
                     /locus_tag="I0B46_000696"
     CDS             14132..15061
                     /gene="cpaF"
                     /locus_tag="I0B46_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAR2083019.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            15018..16067
                     /locus_tag="I0B46_000697"
     CDS             15018..16067
                     /locus_tag="I0B46_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAR2083020.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16064..16342
                     /locus_tag="I0B46_000698"
     CDS             16064..16342
                     /locus_tag="I0B46_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAR2083021.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16317..16787
                     /locus_tag="I0B46_000699"
     CDS             16317..16787
                     /locus_tag="I0B46_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAR2083022.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16804..17106
                     /locus_tag="I0B46_000700"
     CDS             16804..17106
                     /locus_tag="I0B46_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083023.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            17123..17494
                     /locus_tag="I0B46_000701"
     CDS             17123..17494
                     /locus_tag="I0B46_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAR2083024.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17466..17825
                     /locus_tag="I0B46_000702"
     CDS             17466..17825
                     /locus_tag="I0B46_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083025.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17997..19004
                     /locus_tag="I0B46_000703"
     CDS             17997..19004
                     /locus_tag="I0B46_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAR2083026.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            19029..20213
                     /locus_tag="I0B46_000704"
     CDS             19029..20213
                     /locus_tag="I0B46_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAR2083027.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20650..22233)
                     /locus_tag="I0B46_000705"
     CDS             complement(20650..22233)
                     /locus_tag="I0B46_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAR2083028.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22811..22954)
                     /locus_tag="I0B46_000706"
     CDS             complement(22811..22954)
                     /locus_tag="I0B46_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAR2083029.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23177..23656)
                     /locus_tag="I0B46_000707"
     CDS             complement(23177..23656)
                     /locus_tag="I0B46_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAR2083030.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23729..24013)
                     /locus_tag="I0B46_000708"
     CDS             complement(23729..24013)
                     /locus_tag="I0B46_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083031.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24091..24285)
                     /gene="ssrS"
                     /locus_tag="I0B46_000709"
     ncRNA           complement(24091..24285)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="I0B46_000709"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24357..25643)
                     /locus_tag="I0B46_000710"
     CDS             complement(24357..25643)
                     /locus_tag="I0B46_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAR2083032.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25781..26452
                     /locus_tag="I0B46_000711"
     CDS             25781..26452
                     /locus_tag="I0B46_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAR2083033.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26418..26906
                     /locus_tag="I0B46_000712"
     CDS             26418..26906
                     /locus_tag="I0B46_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAR2083034.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26919..27866
                     /gene="prmA"
                     /locus_tag="I0B46_000713"
     CDS             26919..27866
                     /gene="prmA"
                     /locus_tag="I0B46_000713"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAR2083035.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27867..28631
                     /locus_tag="I0B46_000714"
     CDS             27867..28631
                     /locus_tag="I0B46_000714"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAR2083036.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28864..31077
                     /locus_tag="I0B46_000715"
     CDS             28864..31077
                     /locus_tag="I0B46_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAR2083037.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31088..31534
                     /locus_tag="I0B46_000716"
     CDS             31088..31534
                     /locus_tag="I0B46_000716"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAR2083038.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31705..32964
                     /locus_tag="I0B46_000717"
     CDS             31705..32964
                     /locus_tag="I0B46_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAR2083039.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33053..34000)
                     /locus_tag="I0B46_000718"
     CDS             complement(33053..34000)
                     /locus_tag="I0B46_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAR2083040.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34394..35686
                     /locus_tag="I0B46_000719"
     CDS             34394..35686
                     /locus_tag="I0B46_000719"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAR2083041.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35701..37470
                     /gene="aspS"
                     /locus_tag="I0B46_000720"
     CDS             35701..37470
                     /gene="aspS"
                     /locus_tag="I0B46_000720"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAR2083042.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37579..38454
                     /locus_tag="I0B46_000721"
     CDS             37579..38454
                     /locus_tag="I0B46_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAR2083043.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38720..40603
                     /locus_tag="I0B46_000722"
     CDS             38720..40603
                     /locus_tag="I0B46_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAR2083044.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40678..40962)
                     /locus_tag="I0B46_000723"
     CDS             complement(40678..40962)
                     /locus_tag="I0B46_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAR2083045.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVNMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40952..41686)
                     /locus_tag="I0B46_000724"
     CDS             complement(40952..41686)
                     /locus_tag="I0B46_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAR2083046.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41815..42438
                     /locus_tag="I0B46_000725"
     CDS             41815..42438
                     /locus_tag="I0B46_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAR2083047.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42630..43751
                     /locus_tag="I0B46_000726"
     CDS             42630..43751
                     /locus_tag="I0B46_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAR2083048.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43758..46886
                     /locus_tag="I0B46_000727"
     CDS             43758..46886
                     /locus_tag="I0B46_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAR2083049.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46905..50099
                     /locus_tag="I0B46_000728"
     CDS             46905..50099
                     /locus_tag="I0B46_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAR2083050.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50245..50952)
                     /locus_tag="I0B46_000729"
     CDS             complement(50245..50952)
                     /locus_tag="I0B46_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAR2083051.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            51717..52115
                     /gene="spxA"
                     /locus_tag="I0B46_000730"
     CDS             51717..52115
                     /gene="spxA"
                     /locus_tag="I0B46_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAR2083052.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
     gene            52447..53100
                     /locus_tag="I0B46_000731"
     CDS             52447..53100
                     /locus_tag="I0B46_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAR2083053.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            53326..54324
                     /locus_tag="I0B46_000732"
     CDS             53326..54324
                     /locus_tag="I0B46_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAR2083054.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            54500..56308
                     /gene="pepF"
                     /locus_tag="I0B46_000733"
     CDS             54500..56308
                     /gene="pepF"
                     /locus_tag="I0B46_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAR2083055.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
BASE COUNT        18553 a         9727 c        11688 g        16420 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt
       61 gaactaatag agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat
      121 agttggttat tataaaatag taatatagct gatttggatt caattcatat ttatttataa
      181 atcaaaaata cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta
      241 cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc
      301 agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt
      361 tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga
      421 aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta
      481 ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg
      541 gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa
      601 ttgattctgt cctatcgaaa tttccaacca aaatatatac aaatctcgga agttatgaat
      661 ctcagaatta taaaaataaa atgaaaaaaa tttttcacga ggtagggagt gaattttcta
      721 tcaaagagct aaactatgag cttgagtatg aaaaatctat ttgttcaatc ccttataata
      781 atattgagga aattatagaa aaggatgcta tcagcattta caaatttttt atttttattc
      841 ctaatcgtca attgaaagct aatatattta accatttgaa aaagatgcta aatgtgtctg
      901 ttactgaatc ggcacctgta aatattgaaa tcgtacctca aaatgtatct aaaggtgtgg
      961 tgtttgatcg tttggaatca atctacaatt taaagaaacc tttgagaatc gcgatcggag
     1021 atagtttaaa tgacttatct atgtttgaaa gtgcagatat tagttttgca atgggaaata
     1081 gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt ttcaaatgat aaagacggag
     1141 tatctcaagc atttgaaaga attcttacca tataaactaa ttaaggtagt atagaacata
     1201 gaattctaaa tacggaaaaa tggagcataa attccagtac aaatagtact tcttcagtgt
     1261 caaacgaagc gaatataaca tagatattta ggattgtgac aaaaatcatg tcacaatctt
     1321 tttttcgaat aaacttattt tttgaagtcg gccatttatc gtaatttctt tgcataatac
     1381 gaacaaattt tcggttattg atgaaaaaaa acattgaaaa tggtatgata aaagaaaaaa
     1441 aggaggaaat ttttaatggt gaaatttatc catgcggcag atttacatat ggatcggtca
     1501 tttgaaggac tgacaactct tgataaaact gtacaggaaa aactattgaa gacgaatctg
     1561 accgttttat caaatatcat tgaacaggca ataataaata acgttgattt tgttttactt
     1621 gcaggagata attttcatca aaatcgtcct tcattaaaaa tacagaaaca tttttcagaa
     1681 cagatgaaac ggttagaaaa gaaccatata cctgtattta taatattcgg aaatcatgat
     1741 ttctatcaaa aagagcggta ttggttctcc tttcctaaaa atgtccattt atttacatcc
     1801 gaaacggtag agacaaaaaa aataacgata aaaagtggag aaactgtctc tctcagtgga
     1861 tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga tggaatttcc ttctagagaa
     1921 ctcacagatt accatattgg gttgtaccac ggggaacctg gcgtctctca aaaaggaaac
     1981 tatgctcctt tccaaccctc aaagatgcaa gaaaagggat acgattattg ggctttaggg
     2041 catatccatg tgccaacggt cttaaatgaa aagcaaacaa ttgtttatcc aggtgcgcct
     2101 caaggtcata ctaaaaagga acaagcttct acatctattc tactagttga attaagtaaa
     2161 ggaagttgcc aaatacatcc gattaaagta gcagaggtct attggaagac aaaggaaata
     2221 tcattgagga aagcaagaac gacaaaagag gtcattgttc acattcgtca gcagctttca
     2281 aaagtagagg atggatttag tttaattgag ataaaattaa aagattatga ccatctcaat
     2341 acagatgttt tggaacggat acaatcagga gagctattag actatttgtt agaggaattc
     2401 tcagatagga taaatgattt ctttatttgg cgtatttctc tcatagagaa tactttttat
     2461 acgaagaccc cacttgctgc ttctgctaaa ttgatggaac agttatttca atactatcag
     2521 acacctcaag atttccaaca aattttaaat gaagtatata gtcatcaaga agcggctaga
     2581 atattgagcg aactgccgga atatcaagaa gaaacacttg aaaaagccaa gcagctgctt
     2641 aatcaagatt ttttatttga ggaggatcag gaatgaagat tttaaggatc gagattgctg
     2701 cttttggaaa atggcgtcaa aaaagttttg acttttattc aggcaatcag ctgatctatg
     2761 ggggaaacga agcaggaaaa tcaacgattt atcaatttat tcaagcaata ttatttggtt
     2821 tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa agacggctca gcctatggcg
     2881 gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc tgtcgagcgc tacaagcagc
     2941 aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt gggaagtgat gagttactag
     3001 aaaaactgat cgcacctttg acgaaagagt tattccagca ggtatttact ttccagcaag
     3061 agcagttgat ggaactagat catttgagag aaaaagagct acatgatgcg ctgatttctt
     3121 taggcatcac gggcagttct caagtattcc aaaagcggca agaatattat cagaaagcac
     3181 aacaattatt caagaaaaaa ggccaaaaat tactaatcaa tcaaaaatta aatgaatgga
     3241 aagagctgca agaaaaaatt catcaaaaac aagaacagga agcttatttt ggcaagttga
     3301 tccaacaaga acaggaatac gagtcgcaac agcagcagtt attggcacaa ttaaaagagg
     3361 cgaacaaaca attttcatta gccagacagc aagaacttaa tttttcttta tatgaagaat
     3421 ggcaatctct caaaaaaaac agcactacag gtcttcctga tgagcaactt cttttagatc
     3481 tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg tttggaagaa ttagaagcaa
     3541 cactggaaaa gcatagcggg atggatcagc agtcagccag atattatttt tatttagagc
     3601 aggagcagga actaaaagat ctccagcaac ttcaatttac agcggaacaa ttaaacgaag
     3661 aatggcaaag attacaaatt gagactagag aaacaaattc agaattgaag attttggaac
     3721 aaaggtggca ttggcagaaa aatccgttac cggaagaatt tttatatgaa gaagaatgga
     3781 agcatcttct ggctgaagaa agaaggattg ctgatctaga aagccagatt tctgtgcgtc
     3841 tccaaatagc taaagaacag caagagatga ttgaagcaga agtgaatgac ttggaagaga
     3901 cctatccagc attattacaa aatcagtcag agatagcacc agtctcttca tctaaaccgt
     3961 atatgctgat ggctggcgga gcaataatca ttgcaggtat atttgtacca atgcccttga
     4021 aagtgatcct tttactaatt ggtgcaggca tgattggcta tttcttctac aaaaggcaac
     4081 caaaaattaa aactaaaaat gatttctctg agataaaagg aatgtggcaa gaaaaactag
     4141 gacaacttga tctagcctta gctcaattag ctgaagcaga agaaaaacaa aagagaactg
     4201 gagaagaaaa acagattctt cagcaagaag tatatcgttt tgcggatcat cggcacttag
     4261 gaaagatgga tacgctcaaa ctcatgaaag aatatggaca ggaagtcata gattatcaga
     4321 aattatatgg cgtatttgag cggaaacata aaagagaaca ggaaatacaa gaaaaactac
     4381 gacaaatcga ccaaaaattt gagtttgtta aagaatggtt accgatacaa gataagacaa
     4441 tttcagaaaa aatggtattg ctttcccgtt ttatacaaga gatggaacag atgaaatttg
     4501 ccagaagcta tcagcaaaat actttattga agcaagaaat caatcaattg aaaaagaaac
     4561 aaaaagtatg gatacaagaa tacagcgaac aattgactca agcaggctta tcttatcctt
     4621 cggaagtacc tgtcttttta caaaaagcca aacaagccga acagcagatg aaaagataca
     4681 atgaattatc ggaaatgctt actccactat ttcctgaaga gctgacaaaa gagaaattat
     4741 ctgtaagact gcgccaactg aaagaaaagc aatcccaact ccagcaagaa gaaaaagaag
     4801 caagtgaaaa tcggcaacga ctccaacttc aagtagaaga gttgcaaaga gacggcacat
     4861 tggacgaact ttatcaacaa gctagccggc aaaaagcaaa attacaggaa ttactggagg
     4921 aatggggagc acttgaaatc gccgggacat tcttaggtga tctttcaaca gagatgagtg
     4981 aaagacaatt accccagttg ttaagagcag caggtgatta tttaggtatt ttaacaaaag
     5041 gaaattatca gaagatatta ctgaaaaatg atacactgac tttaacagac ggatatacag
     5101 aatttcccat ctatgagtta tctacaggta cgaaagatca gctgattatg gcgatgagat
     5161 ttgcttacct ctcattagaa gctaaccgtt cgatatgccc gatcatcatt gatgatggct
     5221 ggcttcatta tgatcatcag cgaaagtatc aattagctga acttttagct aaatttggag
     5281 aaaagtatca aataatttgt ttttcttctg ataaagaaat ggtaagctat taccatgaga
     5341 tgaaccaaat cgtaaaacaa ttagaaggag caaataatga aaaaaattcg tgatttagta
     5401 atcgatgagc agtttgaaca atttgtcctg atcaaaagtg cagatgtaag aatcgctaaa
     5461 aacggaaaaa aatttattgc ttttactttc caagatactt caggtactat cgatggtaaa
     5521 tactgggatg cttcggagga ggaaatcaat aaatttacag ccggcacagt cgtttttcta
     5581 aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa agattcttca tatgcgctta
     5641 acaagggtag atgagccagg agagcctagt ttatacatgg aaagagcccc tttgaagaaa
     5701 gaagaaatgg aagaagaaat caatcaattg ttgtttgaga tcaccaatgc gcattggaat
     5761 cgtattgttc gctatctact taataaatat caaaaacaat tttttgagtt tccggcagca
     5821 aaaagaaacc atcatgcatt tgccagtgga ttagcttatc atactttgac aatgcttcac
     5881 ttagggaaat caatcgccga tgaatatcct gagctgaatc gagcattgtt gtatgctggt
     5941 atcattttgc atgatttagg aaaggtgatt gaactttccg gtcctatgtc gacagaatat
     6001 acgcttgcag gaaatttgat cggtcatctg gtattgattg acgaagaaat aactaaagcc
     6061 tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc tcgtattacg tcatatggtt
     6121 ttagcacacc atggcttatt agaatatggc tcacctgtac gtccaagaat aatggaagca
     6181 gaaatattac atcaaatcga caatatcgat gcttcgatgc agatgatgtt gacttcgatt
     6241 cgtcaaacag aaccaggaaa atacaccgat cggattttcg gtatggacaa ccgaagtttc
     6301 tatgtaccaa aagatttata gcaaaaattt atttttttca ttcaaaacaa aaaaagcgag
     6361 acataagtct tcgctttttt tgttttcttt tattttgatg aatcagttgt ttcttcagtt
     6421 gaagattctt ttgttgaatc agttgatttt gtgtcagaac tctttgttga agctgtagtt
     6481 tcacttgagt ctttcgtact tgatgaagtt gttgtgaatg cactcaacac attttcaaag
     6541 gcatcatctt tgatttttac atttgcatct tttaattctt caccgattac tttagttgtg
     6601 aatgcattgt cacttagttt agtttcagta gcgatatctt ttagttgatc tttgtattta
     6661 tccatgtcat tgcctttatt ttggtttttc accattttga caacgtagta ttctgtcgca
     6721 taagaagtag gatttgttgt agtaataaca tcggagatct caccgtcttt caatttgaaa
     6781 gctgcttctt ttacttctgc aggaatagtt gttgtcgttg agtcgaattt aactttaccg
     6841 ccatcttctt ttgtctctgt atcagttgat ttgtctttgg ctaatttaga gaaatcatct
     6901 ccatcatccg ctgatttttt cacatctttt gcttcatctt cacttgataa tttgatgatt
     6961 tgtgcttcta cttctggatg gaaagatttc caagcagttt tcaaatcgtc atctgtaata
     7021 tctacgtgag ctttcaagcc agcttcaaaa gctaaattgt tacgaatata ttctttataa
     7081 gtatctttcg tatatccagc agcttctaat tgtgattcaa aagtatcacc taatgatttc
     7141 gcttgtttgt cgtattctgc atccacttgc ttatcggtta ctttatcgcc atatttgtta
     7201 ttgaatactt tataaatgat catccgttgt actaaggatt ggttcgatga ttcaagtttt
     7261 gcttcatcat agaaatcaga tacagtgatc gttccgcctt tcatcgttgc gatatcttta
     7321 ttagtatcgc ctgaacaagc tgctagagtt aaaacagcca acgcactagt agctgccaaa
     7381 ataattgatt tttttttcat taaaatttgc actcctgtaa ttagttatta tttttatata
     7441 ttaactatac catatcccat ttcctttaaa aacgttgtcg tggtagtttc aaacaaaatt
     7501 caaaatttca tcacattcct aggaaatacg caaacttttc tcacttatat agttaaatct
     7561 gttgagaaaa taatcattat tatatttgtt caggatactc tggcagatca gcttttagtt
     7621 tttctacttg ttcattgatt tgtgtgatac gtggttcggc ttggaattta aaatcttcga
     7681 tatctttttt gattgcttct tggaaagcag gaatcacaga ttcagctgtt tcttttgttt
     7741 cgatcaatgc gcttttaaaa tgattcaggc tgttatttaa atcatttgtc agagttgttg
     7801 cttcttccaa ttcttcgatc agtttctttc ttgtttcatt cccgctcctg ggcgccaata
     7861 acagacctcc gacagtgcca gcagcagctc caaataacag tcctttgaaa aagtttttaa
     7921 tcatcgcagg acacctgctt tacgattgtt tcttgaatag cttttagttc ttccgatcca
     7981 taactatctt gatgattgac aaagtgaata gaaaaatcat cctctttgct gtagcgaggg
     8041 actaaatgga tatgtgaatg aaaaacagat tgataggcta gttcacggtt gttattcaag
     8101 atattaagac ctttcatttc tggaaaagct ttttccaatg cttgagcaat cttaggcaca
     8161 cgcgaaaaaa cctctcctgc aagttcggga tcatattcaa aaatatctgc tacatgacgt
     8221 ttaggcacaa ctaatgtgtg tcctttcgtt gcttgagaga tgtctaaaaa agcatagacc
     8281 ttgtcgtctt catatatttt atagcttggg atttcttgat tgatgatttt gcaaaaaata
     8341 cagttttcca tataaaacat cctttctata aagaataaat acttcagctt aactttacca
     8401 taagttaaag ctattgtaag gaaaaaaaca aaataccgtc attttcttcg tggtataata
     8461 aataaaaata aatggcggag gaaaaagaat gagtttaatt atcgatcatg tcacaggggg
     8521 gtacggacat ctccctgtgt taaaagatat cagtttcgat gtacagcctg gagagatagt
     8581 agggttgatt ggactgaacg gagcagggaa aagtacgacc atcaagcaca ttatcggtct
     8641 gctggaagct tcaaaaggaa cgatagaaat caatcatcta tccttaaaga aaaatacgga
     8701 agaatatcgc aaacaaattg ggtatattcc agagactcct tctttatatg aagaattgac
     8761 attgagagaa catatcgaag tcactgcgat ggcctacgat atacctatgg atatagcaat
     8821 gaaaagagca gaaaagttgt tggagacttt tcggttatct aatcgtcttg actggttccc
     8881 agctaatttt tcaaagggaa tgaaacagaa agtgatgatc ttatgcgcct ttttgatcga
     8941 gccttcttta tatatcatcg acgaaccatt tttaggcttg gatccactag ctatcaatgc
     9001 attgcttgaa ttgatggtag aaatgaaaaa agaaggagcc ggtattttga tgtctacgca
     9061 tattttagct actgcagaaa aatattgtga tcgttttgtg gtcttacata acggagagat
     9121 acgagcagac ggttctcttg agatgttgag aaaagaattt cagctgccgg attcttcttt
     9181 ggacgaaatc tatattgctt taacaaagga gcaggcacat gagtagtttt tttcaaaaac
     9241 gtttagcaag acatcaaaag aaaatgatga aatatatgcg ttatgtattg aacgatcatt
     9301 ttgttttagt ttgtcttttc ttgattggcg gtgtgggatt atattattct aattggttga
     9361 aaaccctgca gccaccgttt gcaattggag ggctcataat ctctgtattt tggatgctct
     9421 gtttattcgt gggtaagatg gctacttttg cagaggcagc agatattgtt tttttacttc
     9481 ctaaagaaaa agaaatgcaa acctatttgg aacgagcttt tcgctattct tgcatttttc
     9541 cgttgatctt tttgatattg gcatgcggat tggccatgcc attagtggtg gttagtacgg
     9601 agcaatcctt ttcgatgttt ttcgtctatc tgatcatatt atggagtttg aaattcagtc
     9661 atctgcaaat caagagagca gaattgtttc gactagatca acaagttatt cgttattgga
     9721 aattaggctg gtttttttct tctctgatct ttttgtttct atcattttat tggctaatat
     9781 gggcaggaat agcaggtgca ttagtacagg caagtctata ttactggttt ttgtggaagc
     9841 aacaagaaaa aggattggat tgggagaaaa tggtgcatgt agaagaacat aggttgcatc
     9901 agatctatca atttatcaat ttattcacag atgttcctga aattacggca aagatcaaaa
     9961 gaagacgcta tttggatcct atattgactc atattaaaag agaatcaaaa aacacttatc
    10021 tttacttgtt tgcaagaaga ttggcaagag gttctgattt tagtggtctt tatttccgat
    10081 tagtaattat aggtgggtta ttgattgccg gagtagtcga cttttatttt agtctgatta
    10141 tcggtttctt atttttatat ttgatcggtt tccaactgtt acctttagcg aaccagttcc
    10201 gctatatggt tctcgttcag ctttatccaa tccataagaa tcagaagaag caggcgattc
    10261 agtatctact cacatgggta ttaattatcg cgtcggttat ttttggtttg attgctgcag
    10321 tcgtattaga tgggcagcag cgatgggttc cgctagcggc ttatttgatt gttactatgc
    10381 tctttgttaa actatatgta ccaactcgtt tgaagaagat gtcagactga aaacaaagct
    10441 gtgtagggta aaaatgagaa tgaaaaatag agcgttgaca gtaagaatgc tgttcgttct
    10501 attttttttg agaaaagctg gttttttgaa tggcaattaa atgaaaaaga acgtatatgt
    10561 gcttgacgtg tagtgttcag gcgactagaa taataagtac tgcaaaacaa agagtaagag
    10621 gacgggagaa tgcatgatgg agtttcagtt agaccaagag tggcggttac gtcccatcaa
    10681 aggcgacaca ggaaaaacat acataggcat gcgtgatgag gacaaagtct ttattaaacg
    10741 aaacacgact ccgatgttgg ctgctctttc aagggaagga attacaccaa aacttgtttg
    10801 gacaaaacga acggggaatg gcgatacttt gacagcacaa gagtggctgg aaggtcgctt
    10861 gctgactcct aatgagattg gtcaaagaaa cgacgtgatt gatgttttgt accatttaca
    10921 tcattcaaat tctcttaaaa ccatgctgac ccgtatcggt ggtcgtgtca tgacgccgga
    10981 gaagatgctg caaaaatatg aaaaagaatt accggaagaa ctccagaaaa atcaatttct
    11041 gcaaattgtc catcgttttc ttcgtgagca gatcccgtca atagataagg aaaacttatc
    11101 agtagtacac ggagatgtaa accatcgtaa ttggttagta tgtaaaaatt atttatatct
    11161 agtcgattgg gattcaatca tgtttgcaga ccctgcggtg gatattgcaa cgattttagg
    11221 aaattatgtg ccactgtcta gctggaatca gtggctgatc agttatggca tacgcccgac
    11281 tgacgaaatg cttgaaaaat ttcattggta tgcggccatg aatttgctcc aagaaatcac
    11341 cagatattgc aaacgtgaag atcatcgacg gatgaacgaa gaaattttac aattaaaacg
    11401 aattttcagt ggctgaggtt tggcccaaac tctgcggaga tttggccaga cctttttaat
    11461 tagaggaaag gaatggagat aaataatgcg tgtccgaaat cgtccgggag cagcggaaat
    11521 gttagcggct cacccgaatt ttgtaattag tgatccaaca ttgtggaagg gaaaatggaa
    11581 tgaattgttt gaaaacgatc atccaatcca cattgagatt ggtatgggga aaggccagtt
    11641 catcactgga atggcaaaag cccatcctga aatcaattat attggagtag aaatgcaggt
    11701 cagtgttgtt tcgattgcac tagacaagtt gatcgaacaa ccgcttccta acctgaaact
    11761 tctacatgtg gatggatcag cgttgacaga gtatttcgct gacagcgaag tagatcaaat
    11821 ctatctgaat ttttccgatc catggccaaa aaaacgtcat gaaaaacggc gattgacata
    11881 taagactttt cttgctgtag atgaacagat tcttcgtcca aatggagaaa tccatttcaa
    11941 aacagataat caaggattgt ttgagtattc tctggcaagt ttttctcaat atggcatgat
    12001 cctcaaacag gtatggttag atttacatca aagtcaattt gaaggcaata ttatgacgga
    12061 gtatgaagaa aaattttctt caaaaggtca acgtatttat cgagtagaag ctcaatttca
    12121 agataagacc aaaaagaaaa gtctgtgaaa ctaactttct gctttcataa aaacattaat
    12181 atccataaat aaatcccaga aaatgaacat tacagatgta atgtattttc tgggatttat
    12241 ttgatttaaa atgtatttat ttcatcggtt tttccacaag gtggaatcaa ttggaagcga
    12301 gaaaatgaaa ataatccttt gtcggtgatc ttcaattcgg gtatgacaga taatgttaag
    12361 aaagaaagtg tcaaaaaagg atcaaaggtt ttagctgctc ctagttgatg tgctttagtt
    12421 attaattcat tcaactgttc atagacgatc gaaaaaggtt cctgtgtcat gattccgcct
    12481 ataggaagag aaacacaagc tatttcctgc tgtttgttga caacggtcat cccgccacct
    12541 ttttgaatca aaagattagc agcatacagc atatcctcgt cgtttgtccc aacgacaact
    12601 agattatgcg agtcatgggc aatcgttgtc gctatcgctc cttgtttcag ctgaaatcct
    12661 ttcactatgc ctaatccaat gtttcccgtt tgatggtgac gctcgatcac cgcaatcttt
    12721 aattggtctt tttcgattga aggaatgaat cgttcgcctt gtcggtccac tttttctacc
    12781 aaatctttag tcactagact attcgggata atttcgatca catgggcttg tgaagaagaa
    12841 agaagcagct ctaacgaatg tggatttaat ggttgagcgt ttagttttgg cagacaacac
    12901 gtatatgaat ttgaatgatc agcaaataaa tggtcttgga gttgcccaga ttgaacaact
    12961 aatgttccct tatagaaaac ttcatgaatt ggcagcttgg taagatcgtc tgttaagaag
    13021 aaatcagctt gatacccagg ggcaatcgca cctagtgttt tcaaatcaaa acattctgct
    13081 gcattcaatg ttgccatctg aatggcttgt aatggatcga ttccaagtgc aatagcttga
    13141 cggacgatat gatcgattga tccttctttt actaaatcat tgatcagttt atcatcggtg
    13201 acaaagaagc atcttcttga attttcaggt gtgatcgccg gtagtagcga tgccagatct
    13261 tttgcaacgg tcccttctcg aaccattaaa tacataccaa gatccaaacg ttctttagct
    13321 tcttttgcag ttgtagcttc atgatccgtc cgaatacctg ctgcaggata aacatttaac
    13381 tcttccatgc cgataccagc tgcatgcccg tcaatttttt tcttatgctg gtgcatcaat
    13441 tggatctttt ctaggatgtc tgtttcatta tttgctactg cttgatagtt catgacttca
    13501 gctaagccta aaacttcggg tttttgtaaa aaaggggcta atttttttgc atttaaattt
    13561 gcaccattgg tctcaaaagg ggttacaggt acacaagaag gtagcataaa aaagatattc
    13621 atgagtgttt gtcttgcatc ttctatcata aactcaagac ctgtactccc agcaatgttt
    13681 cctatttcat ggggatctgt cactacagta gtaactccat gaagcaagga tacttttgaa
    13741 aattcgcttg gtgataaaag tgagctttcg atatgcacat ggccatcgat gaatcctggc
    13801 actagatatt gcccattagc attatgtacg ctttttcctt cgtattcacc gattcctacg
    13861 atcattcctt cgcagattgc tacatctttt tccatgattt ctttcgtaaa gacgttgacg
    13921 atcttagcat gcttgatcac taggtcagca ggcttttttt tacttgctac ttcgatatat
    13981 ttccttaatg ttgtttccaa tggagtgcct cccaagataa atttttataa gtataacata
    14041 aattcggttt ttgccaatat ttttttgtaa attcatttta tttgaaacaa aaagatcaaa
    14101 aagacgtatg ttttttgtgg aggggagaat aatggaaata aaacgattat cggatcaaat
    14161 gataagtgaa agttttgcca aacatgccca agacgtatat atattgccag cttcagaagt
    14221 attttacatc tattttcgct atggaaaaaa acgaacattc tggaagaaag tctcttcggc
    14281 agatggacag caactgatct caagatttaa gtatttggga cagatggata tcggagaaaa
    14341 aagaaaagct cagttaggtg catttactta ttctttagaa aaacaggaag ttcgtctgcg
    14401 gttgtcttct gtaggagatt accttcaacg agagagttta gtgattcggc tattatacgg
    14461 tacttttgaa acaccccatc gttgctttag ttctcgagat tttgcaacta ttttacagaa
    14521 aaccgtcaga cgtggattat accttttcag cggaccagtc ggatcaggca agacttctct
    14581 aatgtatcgg ttggctttgg aagaaaaatt gcaagtgatt actatcgaag atcctgtaga
    14641 gatagaagaa ccgttttttc tgcagctaca gatcaatccg aaaatagcac agacttatga
    14701 tacacttttg agactcgcat tacggcatcg tccagacttg ctaatcattg gcgagataag
    14761 agatgaatca actgcacatg ctgcggtaag agcagcacta acagggcacc gggtttttgc
    14821 aaccgtacat gcacgcaatc ttgaaggtac attggtcaga atccatgaac tgacaaagaa
    14881 aaaagaagag ctatctgaat gtttagctgg tgtcatttat caagaacttt tggaagatgc
    14941 caatcaccat ccttctgttt tatggagtta tcaatttatg gaggaagaat cagtaaaaaa
    15001 atcatgggag gaaagttatg aagaagctca ggaaaatatt tggcagtcag aactattcta
    15061 aaggattaac aagaagccag caaaaccagt ttgcacaaac catgggagaa ctgcttttat
    15121 ctggttttag tctgcaagaa tccctttatt tattactcac gatccgttcg tttccacctg
    15181 ttattttggc aaatatccaa catcagctaa aggaagggca agcgtttagt gaggtacttt
    15241 cgcaaatagg atatggagca gagcaactgg tacagatcga gttagctgaa cgtcatggga
    15301 atctgccgac aactttgttg gaaattgcca aacagtttcg gcttgttgaa ggatttcgca
    15361 atgatttgaa aaagctgctt gcatatccga tgctattgct cacctttttg ctaggtatct
    15421 tgtttagtat gcggttgttc gttttgcctc agcttattgg ttcaggaatg gtcatgcaag
    15481 agcattgggg tattcagctg atacaagtct ttcattggta tgttctacta ttcatcctag
    15541 cggttactct attggcttta ctagtaaggt atcgtctgat gaagttgtct cctgttgatc
    15601 agagtgaatg gataagcaga caattattga ttggaccgat atattccttg tatcaatcgg
    15661 cttatttgtc actagagatt ggaaagcttt ttcatgaagg tttcgaatta aggcaaatca
    15721 tagtctgctt aaaggaaacg aaacaagaaa gtctgattca gcatcttgcc tctaagttga
    15781 ttcaaggatt ggaggcaggt atgtcattgg ctgagcagtt ccagaattat cgttttctat
    15841 cagaagaatt cagtaaaatt gttttacagg gagaggcaaa gggaagttta ggtaaagaac
    15901 tgatttttta cagcgaatgg acaagaaaac agcttttcaa acgtcttcat cactggatgc
    15961 attttattca gccaatcatt tttttgggcg tagcggcttt gattttatta gtttatgccg
    16021 ctgttttatt acctgtttac ggaagcatag aggaggtttt gccatgaaaa agagaaaaat
    16081 atcttattat agcggtttta cattgatcga aatgctaatc gtcttactga tcatcagtgt
    16141 attagtgttg ttgttcgtcc ctaatttgtc gcgttatagg aatcatgtag accaagaaag
    16201 tcgtgaagcg atcatccagt tggtagacac acaaaaagag ctctatgcgc ttcaaaataa
    16261 tggaagagtt cctactgtgg aggaattgtt gaatgaaggc tatatcaaac gagaacatgc
    16321 agaaatctat cagcggcctt gaaggtaagg ttttagcatc aggatatacg ttgtttgaat
    16381 cactcttact gattttatta cttacaacga tcctttcttt tccagttctc gcattttctg
    16441 catggaagaa tgaattggca gtctatcaat ttttcggcca atttgaaaaa cgaatctatt
    16501 ctacgcaaaa aatcgctatt gtgaatcaga tccaaacagg cttttattgg aatagtgaag
    16561 agaatcaaat catttttcat gttccgaatc cagataaaat aaattgggaa gtattagata
    16621 ttcctgatga gattaccttg aaacgccatg cttctattac ttttgctgct agaaccggga
    16681 atgaaagttc actaaaagct tatcaatttt attgggaaga aaaagagcgg acgattacgt
    16741 atcaattcca gatagggagt ggtcgctata caaaaagaat tgaataaaac aggatttgtt
    16801 ttgttggagt cccttacagc gcttatgatc gcttctgtct gtgtctactt agtgagcttt
    16861 ggccagctgt ttttgcttca ggcagaaaag aaaagtgggg aggaactgct atttattcga
    16921 atgatttatg aagatatcaa gacgcagcgg atgtatcaca aaagaccaga gcgaagggaa
    16981 gaaatgatcc catacgatat ccaaattgtc gataataatg gagtaatcga aaaagtgttt
    17041 gtcaatatgg agaaagaagc gatcagtatt gaaaaaacaa gtaattccag catttacgct
    17101 gattgaatgt ctaattggac tgatgatatt gagcagtcta ttgctcacat tcaatctttt
    17161 gattcagcaa accatccaag taagtgaatt tttgcagcgt aaagatcaaa aagagtggct
    17221 aatattttta gcacaattag aagaagaact caagagcagt cacaatgtct ctgtgaaaaa
    17281 tcagcaactg cactacacga tcgaggataa acagtatatc atcgaaagat atcaggcaat
    17341 gatacgtaaa agaagaactt ccggcggaca tcaacctatg ttaacaagta tcactgagct
    17401 tcaattattg gaaaaagaac acacaatttc tttttatgta cactttgaaa atggagaaga
    17461 tggatatgcc atatggacaa aaaacgatca gtagaaaatg ttataagggc tctattttat
    17521 tttctgtctt gcttttattt cttttatgta cgtttttatt gatggcagca ttagaaagct
    17581 accgtttgtc aatggatctt aatatacgaa tcaaaaatta ttatatagca aaaataatga
    17641 agcagatggc tttatcggaa cagtctacgc ttgccgaaag ttcggaagga gttttttact
    17701 acacaactgg atctgtaaca tatcagtggg tacaacagtc cctattctta gaggtggaag
    17761 tctcaccgtt tatctttcgg ttcacagaag aagtaaaaaa tgatacagat actagtacag
    17821 aatgatggaa tgttcgtgaa aacgccctct tttctgttat aacactgttt ttaataaaaa
    17881 aatgtttgcc gtttcacaaa taaaaaacag tgaaaacgat tgaaattttg tcaattgttc
    17941 gatagaatta aacaggtgaa aaaattgtta taatatatct agaaatgagg tgcaccatgt
    18001 taccagaaaa aatggaacaa gggtttaatc aaaacctgga agctattcag cttttacaga
    18061 atgcattggg aacttccttt ttagaagcct atgtcgaaaa tgcagaaaat ttaatagatg
    18121 attatcaagt acgtgtggtg gatggagtgc cgacaaaaga gacaacgcaa cggatcacag
    18181 ctctttatga agagttgaag aaactttctt tcgagccaga agaatggcgc agactgtctc
    18241 aattactttt attgaagggc agccagacag aacatttgca ggcgaaccat caactcactc
    18301 ctgatagtat cggtttttta tttgtttttc ttattgaaca actatacaca aataaaaaag
    18361 caccggttaa gattctggat attgcggcag gaatgggcaa cttgttatta acagttcttt
    18421 taaacctatc gaatgcgggg taccaaacag aaggaattgg tgtggatatt gatgatacat
    18481 tgttggctgt cgcagcttct acaagtgatt tgacacaagc aaatgttcaa tactttcatc
    18541 aagacgggtt gcaggaatta ttaatagatc ctgtcgattt tgcaatcagc gatttgccaa
    18601 tcggttatta tccgaatgac gaaaaagcaa aagagttttt aacaagtacc gaagaaggcc
    18661 atagttatgc acatcattta ttgctagagc aatcaatgaa atatgtgaag ccggatggtt
    18721 ttggtttgtt tttgatgccg agtggatttt tagaaacgga tcaaagtgaa gaaatcaaaa
    18781 aatggtttaa agaagaaggt tatcttcaag gaatgattca attgccagat gaattgttcc
    18841 gtaataaaca atcgcaaaaa agtattttga tcttacagaa aaaaggacca caagcacaac
    18901 aagtaaaaga agtcttgctt gtgaaacttg cttcattaaa agaaccagag aaggtgacgg
    18961 aatttttcaa cgagtttaaa aactggaaat catctgctct gtaatcattc aacgagaagg
    19021 agagtcatat gtctaaaaca attgcaatca atgctgggag ttcaagtttg aaatggcaat
    19081 tatatcaaat gccagaagaa accgtagttg cgaaagggat cgtcgaacgt atcggtttac
    19141 aagattctat ttttaccatc aagtacggcg ataatcaaaa attcgaacaa atcatggata
    19201 tcgaaaacca cgaagtagca gtaaaaatgc tgttagatca gttgattgaa ctgcaaattt
    19261 taagctctta tgatgaaatc accggagtag ggcaccgtgt tgttcacgga ggagaaactt
    19321 atggggattc agtagtgatc gatgagaaag taatggaccg cattgctgaa ttagcagaat
    19381 ttgctccttt gcataatcct gcaaacttaa tggggatcaa agcgtttaaa aagattttac
    19441 ctgacatttt gagtgtggct gtattcgata catctttcca ctcaacaatg ccaaagcaaa
    19501 attatttata tagcttaccg gtgtcttatt atgaagattt caaagtaaga aaatatggct
    19561 tccatggcac aagtcatcgt tatgtgtcgg aacgtgcggc aaaattattg aacaaaccga
    19621 tcgaagaact aaaaatcatc acttgccact taggaaatgg tgtatcgatc acagcagtcg
    19681 atggaggtaa atccatggat acttctatgg gattcacacc attagctggg gtgacaatgg
    19741 gtacacgttc aggtgatatc gatccagctg ttctcccata tttaatggaa aaattagaga
    19801 ttgatattga tgaaatgatc aatgttttga acaagaaatc tggtctgcta ggcctaacag
    19861 gattgtctag tgatatgcgt gatcttgaaa agaactatga aaaagaacat attcgtttag
    19921 cttatgacat tttcgtggac cgcatccgaa aatacatcgg cggttatgtg acaacgatga
    19981 atggtgttga tgcaattgtc tttactgctg gtatcggcga gaatgacgga catgtacgta
    20041 gtgaaatcat caaaggtatg tcttggtttg gttgtgaaat cgatccagcg ttgaatgaat
    20101 tgcgtggtga agaagtagat atctctacaa aagaatcaaa agtaagagta ttagtgatcc
    20161 caacagatga ggaactaatg attgcccgtg atgtggagcg tctgcgcggc taagccaaag
    20221 cattcgttag gataaaagat gaaacgcatg caagagggaa ttcttgcatg tgtttttttt
    20281 gcggagaaga ccgcaaaatg gtaatttaac ttcaatttcc cttctttttt acaggatctt
    20341 cttagggaat tttgcttgga aaagtggtca aaagggaaaa gactggcatt ttatttattg
    20401 attttcagga aaatcccaat attttatttt cattcatctt tataagggac attacatatg
    20461 taaaaagaaa aaacattttt agatcagcct cgtgggctgt cgactttatt cttcgcttta
    20521 tgtttagcta gaaaaccgat ccgaaacatt cattgatcga ataataaaaa cttgattttc
    20581 cacctgtact ttgcctgaat cgtgaaagca caggtatgaa aatcaagttt tttgttgcga
    20641 aaaaacgtcc tagtcaatca gttggccgag cggctggcgg ctgatctgct cctgatcaat
    20701 aacgagtgct tcttggaagt gttgctgcaa aaagtagcgg tcatggctga ctgctaggag
    20761 cgttccttga tagtcttgca gcatttcttc aatttcttct cgagcatagc tgtcgaggtg
    20821 attggttggt tcatccaaaa tcaggacgtt gattttttct aaaaagagtt tcagcaacgc
    20881 caaacggatt ttttcaccgc cggataaatc tttgatccgt ttacccacat cgctttgata
    20941 aaagccactt ttggctaatt gccgtcgagc gacttcttcg tttccagtca atgtgatggc
    21001 gtaagccagc aaacggctat cgggatcggc aaactgcagc tgctgcggca aataaccgat
    21061 ttgcaagcta ctacctaatt tgatggtgcc accgtaaggc ttttcctcac cgagtaataa
    21121 tttgagtaag gttgattttc ctgcaccatt gtcgccgatg atggctaaat gatctccacg
    21181 atagactgca aaactgctgt ctttgaatag tacttgatca gcgtactgct ggtgcaagtt
    21241 ttgaatgatg aaaacttctt tgccagattt tccaccatta gacaagcttt gcagccgatt
    21301 tttcggggga ggtggcggtt tgacgagtgt cagtttcgcc aggcggcgtt ccagttcttt
    21361 ggcttttttg aagaaggact cattgtctcc ttcatgtgcc cactgacgaa aacgacgaat
    21421 catgactttc aaccgctgga cttctttttg ctgcagttcg taattttttg tcagctcagc
    21481 caagcgagct tctttcaaga ctcgaaaacg gctgtagttt ccgggatact caatcaatgc
    21541 accttcttcc agttcccatg tttttgtggt gacttgatcg agaaattgcc gatcatgaga
    21601 aacgaccaga accgattttt ttgtattctg taaatagttt tccaaccagc gaatactgtt
    21661 aagatctaaa tgattggtcg gttcatcgag taaaagtaaa tcggcatcgg ctgctaaaat
    21721 tttggctaaa gcgactttaa ctcgttcgcc tccgctaagt tctgttaacc gtgcttcctg
    21781 acggtgtgga atccctaagc cttgcaagat cgctgcaatc cggtcttcta atacgtaacc
    21841 gcctagttct tcaaagcgat gctgcagctc accgtaacgg gccagacact tctctaagtt
    21901 tgtatctggt tctgtcatca atgcttcgta gcgactcatt tgctgtttga ggtcctgtat
    21961 ctcagcggca gaatggagca agtagtggtt gacggtcgct tcggaaactg taagttcttg
    22021 ttcgaccgtc ccgatcttta agcctttttt gcgactgata acaccgctgt cgacaccttc
    22081 tctgccggtc aagatttgta aaaaggttga tttgccagtt ccgttgatac cgactaaggc
    22141 aattttttca ccagcttcaa tttgacagga taaatcctca aataatggat tcccgccgat
    22201 tgttttttta atagaaattg cttgtatgat catgatctct ctccttgaga ccactcaaaa
    22261 aggccatggt tatccatggc ctgtgttatt taaactcaga ttatcatgag aaaaatacct
    22321 tgagatggcc attgatttta cgtgaattca actgttttgg acaaacttgt cccgtcaaca
    22381 gcaactgtaa aaccaattgc atggctaaat gcgatatggt atcgttggta attttcatta
    22441 cttgttattt tagtcatgcg catcatagta ttttccctcc ttcgtgtagt tgtcttttcc
    22501 agtgtacctg aaagtgtcag aaagttcaac tagcgctgac atttacttaa tatttatcga
    22561 tgtgtaattc tgtcagagag ctttttttat ctgactgagg gtaatgtctc gtggaccaca
    22621 tcattgtatt ctgagctaaa ccagcgttca ggcgaaatca tatggtgttt gataaaaaac
    22681 tttgtctaca tgacttcctt ttgccatttg cttaataccg ttgcattaaa cagctcttct
    22741 ctttggaaat ctcaccaaca tcgcgttcag gagtatcgtt gtggttgcaa tggcaagcgg
    22801 tagcctttca tcaattcttg aatgatttac cgaatgtagc acttgccttc tcgcggtacc
    22861 cgaacccgaa ataaaatttt agtttggcga taaaatcggt atccgccatc gatacttcat
    22921 attttttcgt atcagattca tcggaaactt ccacattgac gaaccaataa gctttggcat
    22981 gcttaggtcg ttttaaacca caccagggcg actaatcgcc ctggtgttca ctttttttta
    23041 ttttacttga actcattgac tgttttccct ttattttaaa cattataaag accgatgagt
    23101 gcttgccgcg tcttttgttt ttatcacggt tttttgtgca aaaaaacctg ccgatagttg
    23161 gcaggtcgtc tacgtgttaa gctttggtgt ggtctttagc tggcaattgg ttttctaaat
    23221 cagttcggac aacaagcaca tcgcaagctg cgtttctaat aacatattca gaaacagaac
    23281 caataaataa acgttctaca gcattcaatc ctgtggctcc taacataatc aaatctactt
    23341 gattatcttc tgggatttct ctggcaataa tttgttttgg tgagccatat tctacgacag
    23401 aggttacatt attcaagcca gctttcttcg cattgctttc ataatctgcc aatgtttgct
    23461 ttgccatttc agtagcttgt tctgcaagca ttccatcaaa ggatgaaacg gtttggaaag
    23521 aacgtgtgtc aatgacatgt gctaatagaa gttctccgtt atttcggata gctacattga
    23581 ccgccttttt aaaagctaat tcagcttcat cagaaccatc tacggcaacc atgatttttt
    23641 tgtattgttg taacataagc attcactcct caagtcaatc gtattgtact cttttattgt
    23701 agaacacttt agggaaaaag agaattattt actgctttgg ttcagcaatt ttttttgcaa
    23761 atcggatact gaaggtggca gaaacaataa ttacgatcag taaaaagacc aaggcaataa
    23821 agcgttgatt gaaaaaagct gttaaaatac ccattacgcc taaaacagca tatgcagctc
    23881 cgtaaaattg taggaatcct tgatttttat catttttgtt gatcaataca aaaaagatat
    23941 cctgtttatt aaataaataa tagctaacga ataataatac taaagtggcg ataatcatta
    24001 aaatcctaat catgaacaaa tcctccaaaa taagcaacaa atagaaaaag caatttgcca
    24061 agagcaaatt gctttattct ttaaaagatg gtaatctccg ttaatgccgt tgtttgtcct
    24121 gtagtattga tcccgcgaat tcaagcaggt gggtcccatg gtcgtagctt cgaactacca
    24181 aatgataagg catgttacca gcagtgacac aagacgggtc ccaagatatc aataaaaatg
    24241 ttcggtcaac acacaaagag gacaattcga acatcacaga tttcccacct ctatagtagc
    24301 atatgaattt aaaaagagca atgagagagc tcgcagaaac agaaccgttt tcttgtctag
    24361 ttttttggag gatgcttttt ccactctttt atacggtaat actgttgacc cagagcctgt
    24421 tcatatttcc ctgtatcttt tggctgataa tactgagcat tcttcagttt atctggtaaa
    24481 tattgctgat tgacccaagc ctgatcaaaa tgatgaggat actgataacc cacgccacgg
    24541 ttgagtgatt tcgcaccttt gtaatggcta tctctcaaat gatcaggaac atctccagct
    24601 ttgccctctc gaatatcagc aagtgctgca tctaatgcca tgtaggcaga attggatttt
    24661 ggcgacaagc aaagatcgac gacgacgtct gctagtggga tacgtgcctc aggcaatccc
    24721 aatttttcag cagctaatac agcgttcact gtacgagccg cagcagcagg attacctaaa
    24781 cctatatctt cataaccgat aaccatcaga cgccggcaaa tacttgccag atcacctgcc
    24841 tcaactaaac gggcgagata atgaagtgca gcatccacat cacttccacg aatcgatttt
    24901 tgaaaagccg aaataacgtc ataatgagca tctccatttt tgtcatgggt aagagctttt
    24961 ctttggacac attcttcaat gatcgacagt gtaagatgaa tccttccttc tttatctgaa
    25021 ggagtcgata gtgtagccag ttccagccca ttcaatgcac tccgcaaatc gccgttcgtt
    25081 gcacgcgaaa ggtgaagaag agcatcttcg tctaactgga ttgcttgttg tccaaggccg
    25141 cgttctttat cccttaaagc atgttcgact gccaactgga tgtcttgttc tgttaatggt
    25201 ttgacttcaa agatctgtgt acggctgcgg atcgctggat tgatggtaat ataaggattc
    25261 tcagtagtcg ctccgatcaa gatgattcgg ccactttcta aatgaggaag cagaaaatcc
    25321 tgttttgttt tatctaatcg gtgtacttca tccaataaga ggatcactgt accactcatt
    25381 tttgcttctt ccgctacaac ttgtagatcc ttttttgaat ctgtagcggc gtttagcatg
    25441 cgaaatgcat aattagtaga gccggcgatc gcacttgcga tactcgtttt tccagtacca
    25501 ggaggtccat atagaatcat ggaagagagc atgcgagcat cgaccatgcg gcgtatgatt
    25561 tttccttccc cgactagatg ctgctgacca acgacttcat cgatcgttcg aggtctcatg
    25621 cgataagcca atggctgttg cataggattt tcctccttct tgtttttacg tcccttaatt
    25681 atatcatatt tagcaagatg cctagaaaga acagccttct gttgttttgt tttcatagct
    25741 tatccaacta atattgtgta tgataaagta ggtgagaaaa atgacggata aacaaaagat
    25801 agatgaattt ttttggaata aaacgacgcc agaagtggca cgggatttat tggggatgta
    25861 tttggagcat gacacaccag aaggaagatt agctggatat attgtagacg cggaagcata
    25921 cctaggtcca gaagacgagg ccgcccatag ctatggttta agaagaacac cgcgtgtgcg
    25981 ggcaatgtat gaaaaaccag gtacgatcta cctttacacg atgcatactc atcggatttt
    26041 gaacatcatt actcagccag aagggattcc tcaaggagta atgatacgtg caatcgaacc
    26101 cgccgcaatg attgatcaaa tgagtaaaaa tcgtggcgga aaaactggtc cagatatcag
    26161 caacggtccg ggaaaattag tggaagcatt agcaattcct caagaacttt atggtcagtc
    26221 tatagcagat agttcgttac gcttagtttt tgagaaaaaa aagacaccaa aaaagatcaa
    26281 tgctttgcct agaatcggta tcccaaacaa aggtgtatgg acagaaaagc cgttgcgttt
    26341 tgttgtgtcg ggaaatcctt atatctcttt acaaagaaaa aaccaaatcg agaaagattg
    26401 gggttggagg aaagaaaatg aaaaagaagg atctattaac atatttagat gaaataattg
    26461 aaaaacaagt gacagactat gatgtagctt tagattggga tacaaaaaat catacgatcg
    26521 agatcgttgt tcgcttgttt gcagaaaata aagcacaatt tgagattgat gatgcagaag
    26581 ggatcgtttc gcaagaacca attatcgaat ttgaagacgg cgtacttctt tttaatcctc
    26641 agaaatctgt ttttgatgaa cgcaattatc tagcagtaat cccttatgaa ggaaaaaaag
    26701 ggttggcaaa atcagtagct gattctttag tggaatatct aaatgaagta ttagctcaag
    26761 ggcaaagcga tttgcttgat tttttggatg aagacgatga cgaagctgta ttcgagcttc
    26821 actgggataa tcagcaacta gcagaattag tagaagtaaa acagcaaaat gaagcagata
    26881 cttatttacc gtacccaagt tattaaggag tgacagatat gaaatggaat gaagtaaaag
    26941 tagaaactga aagtgaagca gtcgaagcag ttgctaatat tttgatggag gcaggggcga
    27001 gtggtgtcgc gattgaagat tctttagatg ttgaacattt tcagtcggat caatatggtg
    27061 agattttaga taaaaatgat tttacatcgc tgaaagaagg cgcactagtc atggcttatt
    27121 ttccggaaac gactttctta ccggaaatct tgccttttat caaagatcgt atcacacaat
    27181 tacctgagtt tggtctagct attggaaaaa ataaagtaac agttagtgaa gtagaggaaa
    27241 gcaactgggc aacagcctgg aagaaatact atcatccggt gcgtattacg cgaatgctga
    27301 cggttgcgcc aagctgggaa aactaccaga cagaagatcc tctagaaaaa attatcacac
    27361 tagatcctgg tatggcattt ggcacaggca ctcatcccac gacttccttg acattgacag
    27421 cactagaaac agtcttgcgt ggtggtgaaa cattgctaga tgtgggaact gggtcgggcg
    27481 tgctaagtat tgcagcaaaa tatcttggtg caaaagatgt ctatgccttt gatttagacg
    27541 aagtagccgt tcgttcggcg aaagaaaata tggatatgaa tgaaatagcc aaagatgtcc
    27601 atgtatcagc taacgatctt ttgacaggaa tcgagattga aagtgatgta atcgttgcga
    27661 acattttagc agatatcatc ttattgatga tcccagatgc ttggcgttta ctgaagcaaa
    27721 caggcacgct gatcgtttca ggaatcattg aagagaagaa gcaaatggtt ctggatgcca
    27781 tgttagaaca agggtttatc gtggatcaaa ttttccagca aaaagactgg tatgcaatta
    27841 ttttgaaaaa accagaggtg gaatagatgc agcggtattt tttaaaagaa tcatatgaat
    27901 caaaagacta ttttgtttta gaaggggaaa actatcacca cattgtacgg gttatgagga
    27961 tggaacccga ggatgaagtt tttttggcat tcaatgatca aatagccatt gttgcagaaa
    28021 tcacggagat tactgaagag gctgtttatc tgaaagaaat taaaaaagaa gaaaatcaaa
    28081 aggaactgcc aagttcgatt accatagcga gtggctatcc taaaggggaa aagctggatt
    28141 ggatcgttca gaaatccact gaattaggag cgcaagcttt tcttggtttc ccggcatcag
    28201 cctctgtcgt taaatgggat gcaaaaaaac gtatgaaacg acaacaacgg cttgaaaaga
    28261 ttgctcaaga ggcagcggaa caatcccatc ggcagatcgt gccatcagta gagttattgg
    28321 aaacgaaaca agatttattt gagcggctag atgattatga cgtggtcttg gttgcttatg
    28381 aggaatcagc aaagcaaggc gaaaaatcaa atgcagccaa aattcttagt gaaacaaaac
    28441 cagatagtcg aatcttagct gtctttggac cagaaggtgg attatcccgc gatgaagtcg
    28501 attattttgt agaacacaaa gggatacttt gtggattagg accaagaatc cttcgtacag
    28561 aaacagcacc tctttattta ttaagtgctg ctagctatca atgggaatta atgaatcctt
    28621 ctttcagcta acgttcttca ttaataaaag agtatcgaca gaaagaattg aaaaaatagt
    28681 caacaatttg tataatagaa acaattattt atacaatgga tacgaaaaat cgtttgaaaa
    28741 agcttttaaa aagcgaacat ttccgtaaaa aaacgtataa ctgcacggat tttgggacaa
    28801 ttatcaaaca gatatttgtc ccgattcccg ttttaccgaa aaaagaaagg gggatcgatt
    28861 cctgtgacaa aagaagaaat tttaacggga cccggtgtca taaagctcgt tagtcaatac
    28921 atgggtcccg aacatgtagc atttgttgag aaagcatgcg aatacgcgac tgctgcacat
    28981 gacggacagt ttagaaaatc aggcgaaccc tacattattc atcctatcca agtcgcaggt
    29041 atattagcag atttaaaaat ggatccccat acagtggcta caggcttctt acatgatgtt
    29101 gttgaagata cagaaatcac tttagaagat ctgagagaag aatttggcga tgacgttgct
    29161 atgttagtgg acggcgtaac caaattaggg aaaataaaat ataaatccca cgaagagcag
    29221 ctagcagaaa accaccgaaa gatgctgcta gcaatggctc aagatttacg agttatcatg
    29281 gtcaaattag ctgatagact acataacatg cgtacgttga agcacctgcg agaagataag
    29341 caaagaagga tcgctcagga aactttagaa atctatgcgc ctcttgcaca tcgtctaggg
    29401 atcagccgga tcaaatggga attagaagat acagcacttc gttatctaaa tccaaaacag
    29461 tattaccgta tcgtccactt gatgcagacg aagagagaag aacgtgaaaa atacgtaagt
    29521 ggtactgttg aagatattcg aatagcgaca gaagagctgg ggatttttgc agaaatctat
    29581 ggacggccaa aacacattta ttcaatctat cgtaaaatga aagatcagaa aaagcagttc
    29641 aacgaaattt atgacttgct agctattcga gtgatcgtag attcaatcaa ggattgttat
    29701 gctgtattag gagcaatcca cacaaaatgg aaaccaatgc ccggcagatt taaggattat
    29761 attgccatgc caaaagctaa tatgtaccaa tctttgcata ctaccgtgat cggtccggca
    29821 ggaaatccgg tagaaattca aatccgaaca caggaaatgc atgaaatcgc tgaattcggg
    29881 gttgctgcac actgggccta taaagaagga aaaaatgaaa aagtagaacc agatggtatg
    29941 acgaaacaat taagctggtt ccatgagata ctcgaacttc aagacgaaag ctatgatgct
    30001 tctgaattta tggaaggcgt aaaaggagat atctttagtg ataaagtcta cgtcttcaca
    30061 ccaaaaggag acgttactga gttaccaaaa ggatccggac cattggactt tgcatacagt
    30121 atccatacag atatcggtaa caaaaccact ggtgcaaaag taaatggcaa aatggtgcag
    30181 cttgattaca aattgaaaaa cggagatatc attgagatca tgacttctcc aaattcattt
    30241 ggcccaagtc gcgactggtt gaaattagtt gctactagca aagcaagaaa taagatcaaa
    30301 cgtttcttca aagcccaaga tcgagaagaa aatgtgatca aaggccacga atccgtggtc
    30361 aaatgtatta cagatctagg atttacgcct aaagatattt tgacgaagaa caaactgcaa
    30421 gaagcactcg atcgttttaa ttatcaaaca gaagatgatc tctatgcagc tgtagggtat
    30481 ggagaagtta gccccttgac gatggccaat cgtctgactg aaaaagaacg taaagaacaa
    30541 aaaatcgagc agcaaaagca agaagcagaa gaaatcatga atcagccgaa aaaagaacct
    30601 gacaaaatga aagtacgtca tgaaggtggc gttgtcattc aaggggtaga aaacttacta
    30661 attcgtatca gtcgctgctg taatccgatt cctggtgatg atatcgttgg ttatatcact
    30721 aaaggcagag ggatatccat tcatcgtcga gattgtccga atgttcagcc tgacaaacca
    30781 aatgtagcag aacgtttgat tgaagtcgaa tgggaagata catcgaatac acgaaaagag
    30841 tatgatgcag atttggaaat ttacggctat aatcgttcag gcttattgaa tgatgtactt
    30901 caaacagtca atgcgctaac gaaaaatctc aacagcgttg aagcacggac gaataaagat
    30961 aaaatggcga cgatccattt gacggttggt atccagaatt tatcccacct aaagagtatc
    31021 gtggataaaa tcaaagcagt acctgatgtc tacagtgtac gccggacgaa tggataggag
    31081 gacagatatg agagcagttg ttcaacgggt aagtaaagct gctgtgtcag tcgatcagca
    31141 agaaattgga aaaattggct acggcctact tgttttatta ggcattcatg aaaccgatac
    31201 gcaaaaagat gtggactatc tggtaaaaaa aattgcacaa atgcgtattt ttgaagatga
    31261 acaaggaaaa atgaatatta gtgtagaaga tgtgggagga caaattttaa gtatctctca
    31321 attcacactg tttgcggata cgaaaaaagg aaatcgacca agctttgtgg aagccgcacg
    31381 accagaaaca gctattcctt tatacgaatc attcaatgaa ggaatcagaa gtcgcggcat
    31441 cacagtcgct accggacaat tcggagcaga tatgtcggtt tctctcgtca atgatggacc
    31501 tgtaacgatc attattgatt ctaaaaacaa ataaagtaat ctggttttca ctagtaaaaa
    31561 ctatgaaaca cgtcgcttaa gttagactga taaactctaa cttaagtggc ttttttgtct
    31621 gcaaaaagcg cttcttttgt cagcagtcgg aaaaaaagtt aagcttaaat tatacaagtt
    31681 attttttata ggaggttgct gaagatgaaa gaagtaccag tagtagatat caaaatcaca
    31741 gatttaaaaa aattgtatca aaaaggatac aacatgttgg aggagttacg ccatgaagcg
    31801 gacgctcccg tcgtcaaagc aaagattttc aataaagaag ctatcacgat ctacggatca
    31861 tcagcagcaa aagtatttta tgatccaagg aatttcaaac gaaaaggtgc tatgccaaaa
    31921 ttagtattga agacactttt tggtcaaggt ggagtacaga ctttggatgg agctgctcat
    31981 caccatcgga aaaatatttt tatggatcta atgacaccgg aaagaatgga agattatcat
    32041 cgtatcttag ataagaatct gacacaagca cttgaagcac agcatggtca atttgaacta
    32101 tttgatttaa gtaagatggt tttctttaca tctatttgcg agtgggcagg tatcaattta
    32161 tcagcgattt ctaaagatga ggtcgaaaaa ttagcagaat atcaaatctc tatgatcagc
    32221 ggaacgttta cttctccgat tgatcacata aaaggtgtcg aaaatcgcaa aaaatcagaa
    32281 aaatgggcgc aaggcttgat tgaagaagct cgacaaaatc ctgtggctgg aaaagaaaat
    32341 gtggcattat atgcttttgc caatgccaca gatttagatg gtcagctttt acctctggaa
    32401 gtggcagctg ttgaattatt gaacatcatc cgtccaacgg ttgctctaac tgtgtgggcg
    32461 gcattgatgg gacatgcact tttcagtaga ccagatttgt atcagcaatt aaaaaatgac
    32521 ttttcgacgt tacaagatcc gtttattcaa gaaatgagac gctattatcc tttcttccca
    32581 atgctacctg cgatttcgct gaaagaagta gaagtagatg gttatcgtat accagaaggc
    32641 agttgggtta ttttagattt atatggaaca gaccatgatg aacgaacagt tgaagcgcca
    32701 gattccttta tgatcaaacg gtatgtaggg aaagcaaaag atatttccta taaagaagaa
    32761 tatgagatga ttgcacaagg cgggggaaat tttcgacaaa tgcatcgctg tgcaggagaa
    32821 tggatcactc ttcatagtct acgtgtgttt tccgatcagc tagtaaataa gtttgagttc
    32881 tctgtacctg aacaagattg gacgattcca ttcaatcagt ttccgactta tccgaatagc
    32941 cgagcgctac tttacaagaa ttagattagc ataaacttat cgaaaaaaag gaagatcaaa
    33001 cagtgctgat cttccttttt tcatattact ataaattgat aaggtcaatt gatcaaaatc
    33061 cacgaaatgc ttcatcaatg gtttgataat cttcattgga taaatgcaaa tctaaagctt
    33121 ggacattgtc agatacttgc tctggacgct tagctccagg tataactaca gacactcgtg
    33181 gattcttcat gtaccaagct aaaacaagtt gcgcaggagt ggcttggtat cttttagcaa
    33241 tggggcgaag tacatcgaca gcggttaaaa tttctctgaa acgttctcct tggaaatctg
    33301 gatttctttt tcgaggatct ccttcaccaa attgtttttc ctcaccaagt tcatattttc
    33361 ctgtcagcaa accggaagca agaggaaaat agggaacaaa actgattttg tttttttcaa
    33421 gataagggaa taattctttt tcagcctgcc gatggatcaa actgtattta tcttctacta
    33481 catcgacata tccatctgca ttcgcttctt tcaattgttc taacgtgaag tttgacacac
    33541 cgactgctcg gatttttcct gcttctttta attcgtgcaa tgtagcaact gattcatttt
    33601 ttggtgtgct ttcatccgga aagtggatat aaaaaatgtc gatgtaatca gtttgcagac
    33661 gttttagtgc atcttccacc gattgtttga gaaactcagg agaattatca aatgttcgcc
    33721 ctttatttgg tacatgagca gcttttgtag cgatgacgac acgtgagcga tcatattctt
    33781 tttccttcag tacctcgcca atcagttctt cggaacggcc gtttccgtaa gcataagcgg
    33841 tatcgatcaa attgatcccg ctattcaaag ctgtacgaac tacttgtttt cctgtctcat
    33901 cttccaaacc gtcaaaaaga ttatgtcctc caacggcatt tgctcctaat ccaagctgtt
    33961 ctgcgtagac ttgtgaatgt ccgattctta tttctgccat agctgattcc tcctttttgt
    34021 ctagtctaaa acaaaatacc aaaaagctga actaaaacgt ttttgtgagg ggaaatagtc
    34081 agatatctct tgactttgag actggttttt agtaggttat aagtaatatc gaatgtcaat
    34141 gaaagagcga gtaagttttt gaaactttac aagagaggat catcgaggct gaaaatgatc
    34201 taaagtttca ttagcctaaa gacactctgg agatggacga gtgaaaagcc gtcccgcgta
    34261 cgagcgttat tcgtaagagg aaagagaaat ctttcaaaat taggtggtac cgtgtatgaa
    34321 tttcattcac ccttgttcat gatgaacaag ggtgattttt ttatttacat agctaagaaa
    34381 gggagagaaa actatgagtt tccaaagacc aaaaggaaca aatgatattt tgccaggcga
    34441 atcagaaaaa tggcagtttg tcgaagaaac agcacgttta ttattcagag attatcagta
    34501 taatgaaatg cgtacaccga tttttgaaca ctatgaagtg atatcccgaa gtgtagggga
    34561 tacaacggat atcgtttcaa aagagatgta tgatttttat gacaaagggg aacgccatgt
    34621 gacgcttcga ccagaaggta cagcaccaat cgttcgttct tttgttgagc ataaattgtt
    34681 cggcccagag tatgcgaaac cgttcaaaac tttttatatg ggaccaatgt ttcgctacga
    34741 acgccctcaa aaaggacggt tacgtcaatt ccatcaaatc ggagtagaag catttggatc
    34801 agaaaaccct gcaactgatg tggagagcat ggtgatggca cttgatttct tcaaacagtt
    34861 ggggatcacg caaatccggc tagtcatcaa ttcattaggt gataaagaaa cgcgtcaagc
    34921 ctatcgtcaa gcattgatcg actatctttt accttttgaa tcagagctga gtgaagactc
    34981 taaacgccgc cttcatgaaa atccattacg cgtattggac agtaaagaca aacgagatca
    35041 aaaatttgtg gcagaagctc catctatctt agattatcta agcgagcctg ctcaaaaaca
    35101 ttttgatacc gttgtttcca tgctggatgc attggctgtt ccttatgaaa tcgacagtaa
    35161 tatggttcgc ggactagatt attacacaca tacgattttt gaaatcatga gtgacgcacc
    35221 aaaaatgggg gcacaagcaa cgatttgcgc aggcggacgt tatgacaatc tagtggaaga
    35281 actaggtggt ccttcaacac ctggcttcgg atttgcaatg ggtatcgagc gattactctt
    35341 gactatggag gcagaggaag tagttatccc tgcatttaat gagttggatg cttacgtggt
    35401 cgcactcgga gacgaaacaa atatcgaagc attgaaagtg gtccaagcga ttcgaaactt
    35461 tggtttttca gctgaccgcg acttcatgaa ccgtaaagca aaagcgcaat ttaagacagc
    35521 agataaagcg aatgccaaat tagtattggt gatcggtgaa gatgagcttg ctagtcaaac
    35581 gattaatgtc aaagcaatgg caagccgaaa agaaaaaagt tttccattag aagagatata
    35641 tgaacatttt gatgaagtct atgatgaaat gactttattc gagtaggagg aaaaaaataa
    35701 atggcacaaa gaacgatcta ttgcggatta gtttccgaac aacaattaga acaaacagtt
    35761 acattaaaag gatgggtaca aaaaagacga gaccttgggg gagtaatctt tatcgaccta
    35821 cgtgatcgtg aaggaatcgt tcaaatcgtc tttaatcctg aaatcaacaa agaagcatgg
    35881 gaaatcgcgg ataaatgtcg cagtgaatac gtgatcgaag tgactgggga agttagaaga
    35941 cgcggagaaa atgcagtaaa tcctaaaatg gccactggtg aattcgaagt gattgcaagt
    36001 gaaatcgtga ttttgaatca agcaaaaacc ccgccatttc aaattgaaga tgatcaagca
    36061 atcagtgacg aaatacgttt gaaatatcgt tatttggatc tgcgtcgtcc aaaaatgact
    36121 aaaaacatca aattaagaaa tgcgacaaca aaagcgattc gtcattattt agatgataat
    36181 gactttctcg atatcgaaac accttaccta gggaaatcta caccagaagg cgcaagagac
    36241 tacctagttc cttcacgtgt ccatgctgga catttctatg cgttgccgca atctcctcaa
    36301 ttgtttaaac aattgctgat gaatgctgga ttcgaccgtt attatcaaat cgttcgttgt
    36361 ttccgcgatg aagatttacg tggagaccgt cagcctgaat ttacgcaagt agatattgaa
    36421 acaacgttct taacagcaga agaaatccaa acttacacag agggattgat tgcgaaggta
    36481 atgaaagaag ttcgcggtat cgaagtcaca ttgccatttc ctcgtatgac ctatgatgaa
    36541 gcaatggctc gctacggcag cgacaaaccg gatacacgtt ttgatatgga attgatcgat
    36601 ctaagcgata cagtcaaaga agtagaattc aaagtcttcc aaatggctct tgaaaacggc
    36661 ggtgtcgtga aagcattgaa tgctaaaggc gctgcagatc gctactcacg aaaagacatg
    36721 gatcagttag gtcaatatgt aggacaattc ggagctaagg ggcttgcatg gctgaaagta
    36781 gaagaagacg gattgaaagg tccaattgct aaattcatgg gagaagcaac agaagcaatc
    36841 attaaagcaa cagatgcaaa accaggtgac ttgctgatgt ttggcgcaga taaaagtgaa
    36901 atcgtcgctg ctgcattagg tgctatccgg acacgtttag gaaaagaatt aggactaatt
    36961 gatgaatcga aattcaattt cttatgggta actgattggc cacaatttga attttcagaa
    37021 gaagaaggtc gatatgtctc tgctcaccat ccattcacga tgccaaaaga agaagatatc
    37081 ccgttattgg ctgaagaccc tgcaaaagtc tatgcagaag cttatgatat cgtcttgaac
    37141 ggttatgaac taggcggagg ttctatccgt attcatactc gtgagcttca agaaaaagta
    37201 ctagaaactc taggctttac aaaagaacaa atggaagaac aatttggttt cttgttaaca
    37261 gctttagatt acggattccc tccacatggc ggtattgcat taggattgga ccgttttgca
    37321 atgttattag ccggagaaga taatattcgt gaagtcattg cctttcctaa aaacggaaaa
    37381 gcagcagatg tgatgagtga tgcaccaagt atggtttcgc ctttacaatt gttcgaattg
    37441 aatattgatg taacgaatat agaagaatag ccgataaaaa ggtaaaaagg atttgtcgaa
    37501 tacgcttctt ttgacaacct tgatttaatt gaaaaacact ttataatgaa gccataacat
    37561 ataaagcggg gcttttccat gaaaaaaaca ttcgaaaatt gggcttatca tgattacacg
    37621 accaagcttt cggtatctat cgtttatgcg ttgatcgcat cagtcgcatt gaatttcttt
    37681 tatcagccag gacatattta ttcaagtggt attactggtc tagctcagat tttgacgact
    37741 ttgtcaaaat cactgatagg attcgaagta ccggtatcaa caactttgta tttactgaat
    37801 atacctttgt ttgtattggc gtggttaaaa atcgggaaga agtttacaat tttcactttt
    37861 atcacagtca ctttgacctc cctttgtatc catattttac cggaaacagt cctatctacc
    37921 gatccaatca tctgtgcgtt gtttgggggg gctgtgatgg gactaggtat tgggctagcg
    37981 ttgagaaacg gcctatcatc gggcggtcta gatatcgtga gtatcacgat tcgtaaaaaa
    38041 acaggaaagt cgattggttc tatttcgatc tacttcaatg cattgatcgt ttttgttgcc
    38101 ggaattctat ttggctggca atatatgttt tacagtacgt tgtctatttt cgtcagtgga
    38161 aaagtaactg atgtcgttta tactaaacaa aagaagatgc aggtaatgat cgtaacgaaa
    38221 catccagaaa aagtagtaaa tgaaatccaa aactgtatgc gtagagggat cacgatcatc
    38281 aatgaagcag aaggtgctta taagcatgat aagcagacag tattgatgac gatcgttaca
    38341 cgatacgaac tgcctctact tagagaagca atgaaagctt ctgatccaca agcttttgta
    38401 agtattgcag aaaacgtcca gatacttggt cgtttctatg aggatggcat ttagatcgag
    38461 tttgacaaaa attcatgaaa tactgctgta gacagacgtt cgtttgtctg cagttttttt
    38521 ctaccatagt agaatcagca aagaaaaact gcttttttat ttgaaatttt cttaaagttg
    38581 aaaaaaagca tgtttatttt tttaaattta tgggatgata gaaaaaaata gtagagaaag
    38641 gacacgattc tacttgctat tttcacttaa tacccgtata ttacgaatga aaaaagttta
    38701 tgaaaagttg gaatattgaa tggaaaaaca aaaaaggtta aaaaacagcc gctacatcac
    38761 tgggtttgat gggctccgtt cattagctgt ggtgggtgtt atcttctatc accttctgcc
    38821 aactagtatg aaaggcggct atttgggcgt tccgattttt ttcgtcgtat ctggttattt
    38881 gatcacggat ctgctgcggc aggaatggga acaaaatgga aaaatcaata tttggcaatt
    38941 ttatgtcaga agaatgaaga gactctatcc ggcacttgtt tttatgcttg tgacagcttc
    39001 ggcttatatc actttgtttc agcgagggct gttaaacaat cttcgaggag ttgtggtcag
    39061 cagcttactg tatgtcaaca actggtggca aatcaataac ggtttatctt atttcgatcg
    39121 atttgctaat gagtcaccgt ttacgcatat ctggtcttta gcagtagaag gtcaaaacta
    39181 tttggtttgg ccgatcttgt ttgtattgtt gatggtattt gttcgtaaaa gaaaatggat
    39241 cttttatact gtgttaggta catccttcct ctccgccatc ttgatgatga tcttgtatac
    39301 accaggaggc gatccaaccc gtgtttatta cggtaccgac actcgtctgt tttctatatg
    39361 gttgggaagt gcgcttgcgt ttgtttggcc aagtacgcgt ttaaagaaaa acatcccgaa
    39421 acaagcgaaa aatattctga atatcgctgg aggaatctcg cttttggcac tgattctttg
    39481 cttcttcttt ttagatgatc attacaattt catctattac ggaggaatgc tcctagtgag
    39541 tctgttatgc gtagtgctag ttgcagtgac agcacacccc ggagcaagtt tgaatcgatg
    39601 gctgacaaat ccagtattta cttggattgg caaacgaagc tatggcattt atctgtatca
    39661 gtttccagtt atgatttttt atgaagcaaa ggtgactgat attgccgatc atgttttgtt
    39721 gcatacttta gtggaaatca cattgattct aatcatcagt gaactttctt atcgatatgt
    39781 ggagcgtccg ctagcacgtt ttgattatgg tcgtacattc caagtgatga aaggatggtt
    39841 cagcaaaccg atcatcagta agagcaaacc atgggttgtt ccaggcacat tacttgtagc
    39901 aattgcactc gttggtttcg ttacggcacc gaaaaacgct gtaacagccg atcaaaaaca
    39961 actgcaggca aaaatcgctg aaagtaaaaa attagcagaa gaaaccagaa acaattcaga
    40021 aaacaccact agttcggttg accaagcgat ccttgataag tacgagttgt ccaaggcaca
    40081 agggaataag gcacaaaaat tagaactaac ggcatttggt gattcagtta tgttggatgc
    40141 tgcaacggat ctacaagaac tttatccaaa agtagtggta gatggagatg tcggacgaca
    40201 attatatgcc agtgtaccgt acattgaaaa attaaaggaa caagatttgt tgaaagacac
    40261 tgttctgatc ggactaggaa cgaatggctc attctcggaa gctcagtttg atagtgtgat
    40321 ggatgcaatc ggtgatcgaa aagtatactg ggtcaatgtc cgagtaccga cgaagcgctg
    40381 gcaaaatgac gtgaatgcga tgcttgaaca gatgacgaaa aaatacgaca atatgacgct
    40441 gattgattgg tacagctaca gtaatgacca tagtgactgg ttttatgatg atcaagtcca
    40501 tccaaatcct gaagggatga cacattacat tcacttagtc agcgaagcct tgctaggagg
    40561 aagttcaagt acgaacagca gtgagacaaa cgtgacagaa tgatagctac aaaaaacttc
    40621 aggcaaaaac atttgttttt gcctgaagtt tttttatatt taccgtgttt ttaacattca
    40681 tatagattca ggaagcaaaa cggttgagtg tgtctgtaaa taacgttctt tttgttttcg
    40741 ggtgagtttt ttgaatgcag cttctgcttt tgttgcttcg cttcttgaag aaaatacttc
    40801 tgcatggatc atattaacag gtcttctttt ttgaagacga gtatatttag caccagtacc
    40861 attgttatgt tcctctaatc gtctagaaag atcggttgta tatccaccat aaaatgtctg
    40921 atctttacag aataatacgt aaaaataatg ttcatttgcc atagagcatc tcacgaatct
    40981 ctgcagtgta ttctccatcc atattgtaag tgatcaatgg tggagcgact ttgaatccgt
    41041 ctaatttgcc ttctttgatt ccttcaacta acaaaatatt tgcctctttt ccaggttttg
    41101 ggtaaacaaa ttggatcctt ttaggtgcaa ttcgatagtc ttccatgacc tgaatgattt
    41161 ctaagaaccg atcaggacga tggaccatag taaagcgtcc atttgttttt agtagcttac
    41221 ttgaaatatc gatcacttct tctaacgtcg tatgaatctc atgacgggcg attgccaggt
    41281 gtttattagg gttcgtttta tttgttggca gccctttgaa ataaggtgga ttacagacga
    41341 ctaagtcaca agaattatgt cgaacgacag ataagctgtc tttcagatcg atggtgtgga
    41401 cagtcatttg ttcatttaaa tggttaagcg cgatactacg tcttgccatg tctgctagac
    41461 gaggttgaag ttcgatagcg tcgatagccg cctgggttcg tttacttaaa aaaagtccaa
    41521 ctgcgccatt tcctgcacaa aggtcaacaa tcttcccttt tttaggtaca cgggaaaagt
    41581 gagcaagcag cacagcatct aaagaaaaag aaaacacttt ggaactttgg ataatcttga
    41641 tatcttctgc gtataattgg tcgattcgtt cgtcttcgtg aagcatggat tccctccaaa
    41701 atgaaaaaaa tagtcgtctg tattatacta tttttattaa aaagaaccaa ggtggcttgt
    41761 caaacctcta ttttttatgc atactatgac cataagaagg gaaaggaaga gactatgttt
    41821 tatcgattta tgcgcggggt agttcgagta ctattagcct tagttaatgg aaatgcccgc
    41881 tatgagaata aagaagtttt accgcaagat gagaattaca tactagttgc tccccatcgt
    41941 acttggtggg acccattata tctggcggta gctgcagcac ccaaggaatt cagctttatg
    42001 gcaaaagaag aactgttcaa aaatccagtt ctgcgattta tattaactcg atccaatgct
    42061 tttccagtta aaagagaaaa tccaggacca agcgtcatta aaacacctgt aaagattttg
    42121 aaatcgacaa atttgagttt gatcatgttt cccagtggga caagacatgc tacagaactt
    42181 aaaggcggaa tggcattgat ttccagaatg tctaaagcga aaatcgtgcc agctgtatat
    42241 caaggtccgt tgacacttgg ggatctgttc aagcgtcaga gagtgacagt gcgtttcggc
    42301 gaaccaatcg acttatcaga tatcaagaaa acggataaag aaggtatggc ggagatcgaa
    42361 cgacgaacac aagcagcttt tgacaaattg gacaaagaag tgaaccctga tttcaaatat
    42421 gaagtcaaaa aaaaataaaa tgataagttt atgcgtgggc ggtcttttga atctactgtc
    42481 ctgttcagtt cagacttttt caatgaaaac gagggagaga cgtcaagtct gccctctttt
    42541 tgcgttccaa tatgtaaaat agaagacagg tgtcatgtaa tggatcaaag tgttataata
    42601 attagaagga cattttttga ggtgattgaa tgcgtttttt acatacagcc gattggcata
    42661 tcggaaaaaa actacaaggt tatgacttga ttgaagacca gcgtcatgta ctatcttcta
    42721 tactagatat cgcgaaagaa gaaaaagtag aggcaatcgt gattgccgga gacttatatg
    42781 ataggagtgt cccagctgtt gatgccgtag aattatacaa tgaattgatg gtcgattgga
    42841 atttaaagga aaagatcccg atttttgcta tatcaggaaa tcacgatagc agtacgagac
    42901 tttcagcggg tacgccatgg ttcagttatt cgaactattt tttgcgaaca cagctagcag
    42961 aagcatttga accagtcgag tttaaaaacg tacaattttt ctttcttcct tactttgaac
    43021 caattgcagc gagattatat tttgaagatg acaatatccg aacgatcaag caagcggtag
    43081 ccaaggtgat agataaaatg caggagctat ttgattcaga aaaaaaacaa gtattgatta
    43141 gccatttttt tgtaatggga agtgtaaaat ctgattcgga aacgaaaatc gaagtaggcg
    43201 ggttggattc gatccctggt gaactactta caccgtttga ttatgtagca ctaggacatt
    43261 tgcataatca agctgctttg agacaggata atgctcgcta tagcggttcc ccattgaaat
    43321 tctctttatc tgagatcaac caaaaaaaag gcgtatggat cgttgacctg acaacaggcc
    43381 tggagttgaa ttttagggag atcgaacccc tgcatgatat tgaagaaata acagcgagtt
    43441 tcaaagaatt gctggatcct gccttttatc agtccgtcaa tcgggagaat tacatctata
    43501 tctatctcac tgatagaacg atcatcccga atatgatgaa ccagcttcgg caaatatatc
    43561 ctcgtatttt ggggattgaa cgggcaaacg gtcgagcaga acaaacaaag cgcaaacgaa
    43621 aagtactaga aatgaaagat cctcaaaaat tagcagaagc attttttagt gaaatgacgg
    43681 aagagcagct aactgcaaac caagaaaaat ggctcggaga aacgttgcaa gaaatcatgg
    43741 aaatggaatg atcaagaatg aaaccgaaaa aattacaact taaaaatttt ggtcctttta
    43801 tcaatgagac cgtagatttt tcacgtttga cagaagcacc gctttttttg atcagcggga
    43861 agacaggagc tggcaaaacg acgatttttg atgggatcac cttcgcactt tttggtgaaa
    43921 cttccggaag acttcgctct ggaaaagaga tgcgttcttt gtttgctaca ccagacgaag
    43981 aaacaagcgt gactttttct tttgaacatc agcagatgag ctatgaaatc gaacgaaaac
    44041 cagaacaagt tttagcaaaa cgaaaaggaa acggaacaaa aaaacaggca gccaaagtaa
    44101 cgttgaccat ttttgatggg aaaggaaaag aaataaaaca aatcacaaaa cgtacagaag
    44161 cagatcagtt gatcagagaa ctgatgaatc tcgatgcgaa acagttttct caaatcgttt
    44221 tactacccca aggagaattc cgtaattttt tggtttcatc aagcagtgat aaagagactg
    44281 ttttacgtca tttattcggt actcaatttt tccaacagtt caatgagcga ttaaaagaaa
    44341 aagcaaaaca acagcaacaa aaattggatc atttagagca agagctgcat cttctgcaaa
    44401 aaaggtttgt tttagtcaaa gaagaggaac cagcagcagg gtttgaagcc gttttagccc
    44461 aatggacaaa ccatcaggca atactcgaaa aacaaatcac ggaagaaaaa gaagctctaa
    44521 cggaacttca ggaaaaacaa aagcaactag aaaattccta ctatcgtttt gaaagcttga
    44581 aaaaaagtta tgaagaaaaa acgatattaa taaacaagca aaaggaatta gctgctcaaa
    44641 aagacgaaat agaggaaaag aaacgatgga ttgggtatta tgaatttacg caacgattag
    44701 tcgaaccaat ccagcgccta caagaaataa aagttgaaaa aatagaactg tcacaaaaaa
    44761 gtagtgagca aacaaagaaa ctagcgatga ctacgcagaa ttatgaagag tggcaaaaaa
    44821 atgaagcaaa ccgcgtacgc cgacaaacaa aaatcaaaga agctgctcaa aaactgcaaa
    44881 acaaacagat gatggtgcca attgtcgaat ccttacgtag taaaaaacaa gacgcagaac
    44941 aattaacaat ttcattggat aaagaagaat tgaatgccga gaagctgtat caaaatctcg
    45001 ctatctatca ggaaagaact cgaattcttc aagaaaattt actggatcag gaaaaactgc
    45061 aagaagatcg tcttgctttt ggacagttaa aatatctaga tgaaaagcag caagaaatag
    45121 aaaaacaaaa agcaaaagac actgggtcgc tcgcacagat aagagaagaa ctcaaaaaag
    45181 caactgaaga aataacaaaa aatagtcagc tattgaaaga acagacagaa caagcagaac
    45241 agctgaaaag ccaatgggca aagcagcaaa ttgctcgtct tcagcttttt ttgcgtccag
    45301 gggagccatg tcttgtttgt ggatcaacgg aacaccccat acaacaatta aaacacgaac
    45361 agccaaataa ggaagatatc ttaaaagtag aacaacaact ggcagaagct gagtctgctg
    45421 tagaaagaac tcagcaaatc attgccaaaa gtgaagcaca tgaaaataat cttcaacaac
    45481 aattatcaga tttagaacaa aaaatcatgg caacgaatat acaattggct gaacaagaag
    45541 aacacttgca acaggctttg aaaaaggcat ttcctgagat aacagaacaa aaaacaaaaa
    45601 caactcttgt agtaatcgaa caatctttag atagacgaaa aagcaagatg gccaatgcaa
    45661 gaatagagct ggaagaaata agacaacaag cagaaaagct cgaacaacaa ttagctgtct
    45721 acaaacagca gcagacagaa aaaaataatc agctccagca actgcaagga gaaatcaaaa
    45781 gtttagaaca acaattgcaa gatacggata gtaactgttt agaagaggag atacgacgac
    45841 ttgaaaaaca gttagaagaa gactcgaatc aactagctgt tgagcagaaa gcaggagtgc
    45901 aattgttgca agatcttcat acgcttcgga cacagcttga tcttcaacat gaacaaatag
    45961 aaaaaataga tgaaaaaata gaaaaggaac aagaaagaat agctaaaaag ttagaaagcc
    46021 agtcgtattt ttcaacaatt gaagaaatct gtgcatttgc acaacagcaa gcacaatata
    46081 aagaaaacca acgtacagtt acttcttatc atgaagagca attagtgaca gctgaccggc
    46141 tgcgtcaatt agaaaatatc cagctaactg agacattccc agatacagaa gagatcagcc
    46201 aacagttaga agaaataaga cgtatgatcg aaataaagca agaaaagata tacggtttac
    46261 aagaacaaaa aattagtaat caaaaaatct acgatgaatg tctttcgatt tatcacacaa
    46321 gccagacgaa attagatgag cttacgcaat tacagcagct ttcgcaaaca ttcaacggag
    46381 aaaatcctaa gaagactagt ttggaacggt atgtgcttca ggtttatctt caagaagtgt
    46441 tacaagtagc aaatgatcat ctccaacgtt taacgaaaaa tcgttatcaa tttgagctag
    46501 cggataccat tggcagttat cgagggaaaa ccggattaga aatcaatatc tatgatgatc
    46561 aggcaggtat gtctcgaagt gcgcataccc tgtctggcgg tgagagtttt attgcagcac
    46621 tttctttagc attagctctt gccgatgtga tccagacaca agctggtggt gtagcaatcg
    46681 atgcattgtt tattgatgaa ggatttgggt cattagatga agaagctttg gaaatggcaa
    46741 tggaagcttt agaaacgatc gaaagtgaag gaagaatgat cggcatcatt agtcatgtaa
    46801 gagaattgaa agaacgagta ttacaacaaa taagaattga tacagaaggt agcgggcaaa
    46861 gcaaaatacg ctatcaactt ggataaaaag ggagattggt aactatgaaa tggagtattc
    46921 cagaaaaagt cattgcacgt ggacgtcaat acttgcaaga aggccgagtg ttatcggttg
    46981 tccctgacca agaagaaaac gtgtggcatg cagaagtctt gggcagtgaa ttgtatatgg
    47041 ttgatttgga tggtacagca aaagagatag attactgcca atgtccctat tgggaggaac
    47101 ataaatattg taagcataca gttgctgtgg aactttattt acggtcgaaa aatctatctc
    47161 gtatcatgaa aaaagaccag ccaatgctag aaccggtaaa agcaacaagt gagggagaaa
    47221 tcattgcaaa aggtttttct cgtctaaaaa cacaaaacaa tcctcaagcg gtgcaacctt
    47281 tgattattga ataccaagta gaaacgattg agactaatca gtatcaccca gagctttcta
    47341 ttttggcact ttacttgcgt atcggttcat tggcatcacc gaaaaaaaca tatatcgtaa
    47401 aaaacatcta tgatttttta caagcttaca cagaaaaaga aacttacact gttaataaac
    47461 agtaccagtt tcgtctagat aaacgggcgt ttcaaaaaga agatcaaaaa atactcacgc
    47521 atcttgcagg aagtgctcag acgcatcaat tattaggtac gaacggctta caagtcaaag
    47581 gaaaactaga taaaagatat ctgcttctcc cagtagaaca agcccgcttc ctgttagagc
    47641 agatgaatca gactacacgt ttgcatctgc agataaatga acaaaacgat caaggaatac
    47701 aattttccga tcaaaacaaa cctttatcat tcaaagtgga aaaatttgga gaagattatt
    47761 tgctgcaagc attgaatgat tttgattttt tctttatgca ttatcagtgg ggagttatcc
    47821 aagggacatt ttattctctg acgaaatatc aacaaacggt ttatcaaact tggaaacaat
    47881 tgattcgccg attggagaaa ccagaagtag tgttcaagaa aaaagaatta ccgtctttgt
    47941 ttaaagagat tatccctctg ctgtcagaga tcggcgacgt agctattgcc cctgcagtag
    48001 aagaggaagt agcagatatc cccgtcgaat ttattttctt tttccgaaaa gcaaaaggaa
    48061 aaatccaagc tcgagtcgat ttcgtctatg gcgaagtcat ctattcgact gacccgaagc
    48121 atgaggtctc gacagaacag gcttttcagg tgttacgtga cacagtagaa gagaaacgag
    48181 tacttgatct attcagaatg tatcgctacc agaaaaatga aactggttat gaacgtccat
    48241 tgccaagcgg ggaagaacta tatgcctttt tccgcacaga actatctgtc ttccgtcagt
    48301 ttggtgaagt cagacttgga agaaaattgc gagagttgta tttagatgcg cataaacatc
    48361 aaccgaaaat cgaagtaacc gagaccggtt catggctcga tattcgattc gatgtgacag
    48421 ggatagagga gcaagaaatc gatcatgtcc tgcaaagctt gctgagaaat gatgcttttt
    48481 atacattaga aaatggtcag gttctttctt ttgattcaga agaatttcaa caaacaagcc
    48541 aagtgttgca acaattacgg gaatcgatac gtacagaaga aggaacgatc catgtaccta
    48601 aaaatcaagg attgatcatt caaaatcaat tggaaaaaag caatgctact tttagtgaat
    48661 cgttccaaac gatggtacaa gatctcattc atccagaaag ataccaggca cagcttccca
    48721 aaggattgaa tgcaacgatg agagattatc aaaagcaagg ttttcgatgg ttgaagatgt
    48781 taggtcatta tcagtttggc ggtattttag ccgatgaaat gggacttggg aaaacactac
    48841 agacaattgc ctttttactt tcggaaaaag aagaacggaa aagcttctct gctttgatcg
    48901 tagcgcctgc gagcttaatt tacaactggc aggcagaggt gagaaaattc gccccttctt
    48961 taagtatcca agtaatcaat gggaataaaa aagaacgtga agaattactg gctaaagaca
    49021 cagatatccg agtgacttct tatgccagct taagacagga tctagctaat tatcagtcgc
    49081 aaaaaattga ttatttgatt cttgatgagg cacaaatggt caaaaatagc agtacgaaaa
    49141 ctgcacaagc attgagagaa ttagctgtac ctcagcggtt tgcactgagt gggacaccaa
    49201 tcgagaataa tttagaagaa ttgtggtcat tgtttgcaac gattatgcca ggatttttcc
    49261 cgactaaaac aaaattcagg gaacttgccg cagaagaaat tgcgcaaatg attcgaccgt
    49321 ttattttacg gcgagataaa caaactgtgt tgaaagactt gccggaaaaa acagagatga
    49381 atctatattc tgcacttaca gaagaacaaa aaactgttta tttagcttac ttgcgtcaaa
    49441 tgcgtgaaga aatcacatca atggattctg aagcattcaa gaaaaaccgg atcgggatac
    49501 ttgctggtct gacaagactg cgacaaattt gctgcgatcc tcgtctgttt attgaagatt
    49561 accaaggcgg ttccggcaag ctcgaacaag taaaagatct actggtagct gccaaagaga
    49621 acaaacgaag agttttgctg ttttctcaat ttacagggat gctgactatc ttgcaagaag
    49681 agttagccga gctagggatc tcgacatttt atttaagagg cagtacgaaa ccacaagatc
    49741 ggttaagtat ggtagacgca tttaatgctg gcgaaaaaga tgtttttctg atttccttaa
    49801 aggctggggg aactggattg aatctcacgg gagcagatac ggtcatcttg tatgaccttt
    49861 ggtggaatcc tgccattgaa gaacaagctg ctggacgtgc acatcggatc ggacaaaaaa
    49921 atgtggtaga agtgtggcgt atgatctctg aaggaacgat tgaggaacgc atggattcac
    49981 tccagcaaga aaaacgcgaa ctgttccaaa aagtgatcca aggtaatgaa gaacagttga
    50041 cgaaactgac tgaagaagac atccgcatga ttttaagtgt aggagaaatc gaggagtaag
    50101 agactcgaaa aagcgttcag taatgggtaa gaaagccttt ctaaagaagc gattgtttat
    50161 ttgatttaaa aaatagcaag gaggctggga ttccatttta tcccagcttc cttgctatca
    50221 tttttttatt tctattttta aaggttatac aaagattgtc ttcattgtct ctaacagctg
    50281 atcaaaagaa gaaatctcga tatcgcaaat tggagtctgt tcaattttgc gttctcgatg
    50341 gttgaaccaa agcgcttgcc agttagcact ttttgcacct agaacatcat tgtcgtaatt
    50401 atcgccaacg tataaggtct cttctggaag catatgaaat tctttctcag ctagctgaaa
    50461 aatctctttt tctggttttt ggaaacctgt tgcttgagaa atgagcatat tgcgggaagg
    50521 aatccaattg ttcaattgaa gttgcttcag tttttttgtc tgatggtcag tcggcccatt
    50581 tgtgataatt cctaatggga cattcttttc cttcaagaaa tcgagggttt ttttgacctc
    50641 ctcatgcaag cagatattat caagttcttc ctcgtaaatt ttttgaaata agaggccatc
    50701 ttcttccgta atatgagggt agtctaaatc ttttagtgat tggctgatcc gatgtgctcg
    50761 catatattct agtgtccaat ctcctgccat tacttttggg aagttttcat cactatgatg
    50821 gcggaaccgg atatatagtg gatgcatatc tgcatctgaa acaagcggaa cgactctttt
    50881 gaccgcgttt cgaaatggct gctgctgatc atacatcgta tcatcaacat caaacacaac
    50941 tgatttaatc aaaattttac acgcctttcg gttttcattg attttttcac aaatccttga
    51001 ctattgtaac agaaaaaatt gtctaaaaaa gctgataaat aaaggttttt cttactatat
    51061 ttctatttcc ttgacagaat aaggttttta tatttcagtt tttttcacta aaaatcgtga
    51121 aacacgtcaa taagtttgat gaacaaatta atcattatgg tagaagtcga ttgatttatt
    51181 ttaatgcaat ctctgtggaa ttttcgaaag atttattttt ttaatacagt ctcaatataa
    51241 ctattcaaac ataaaataac gttatttgat ttatttgttt atagtaaatt ttatttagta
    51301 atcattgctt taaaatacta cgcttatata acagagaaaa taagcgttat aataaagaac
    51361 agtattattt aaaatgacag aaaacattac tattaaagaa ataaaaaaca taaaaaatct
    51421 taagaaatcg gtttcattta gcttttgaga taaaataata gtacagtttt atacgctgtt
    51481 tcatatgttt agaaaatttg aattagattt gaaaactcta atttttaaat gataaaaaaa
    51541 gcgagaatgc tggttttact gtatattaac cttagataac tttcattttt ttaaaaaaat
    51601 tatgaaaaaa aggtagctat ttaaaagttg attatgtata atagaaacag atgggagatt
    51661 attatgatct tctgttacaa aaaagtgatt cttatcacga aaaggagtgg tgcacgttgt
    51721 taacattata tacttcccca agttgtactt cctgcagaaa ggctcgtgcg tggttgcaag
    51781 aacatcagat tccatttgtt gaacgtaata ttttttcaga accattgaac agttctgaat
    51841 taaaagcgat tttacaaatg acagaagacg gaacagaaga aatcatctcg acacgttcca
    51901 aagtattcca aaaactgaac atggatttag atgatctgcc gttacaagaa ttattggaac
    51961 ttgtgcaaaa caatcctgga ttgcttcgcc gtccaatcat gattgatgat aaacgtctgc
    52021 aagttggttt taatgaagat gaaattcgtc ggttcctacc gcgcgatgta cgacaattag
    52081 aattacgtca agcacaattg atggctggat tatagaagga taatacgaaa cttggaaaag
    52141 actattacaa agatagtttt tccaagtttt tttgttttta caaaaagtat tcttgattag
    52201 atgtataaaa aggtagtaga agatgatgat tgcacgaaaa atagagaggg aaaattagct
    52261 gaaaagtcgg ctatatagta ttcgtctgcc aaaaatgtta tagttaaata agatagcttg
    52321 aaaggttgaa aaatccgctg gttttattaa attcgcttaa cagcgagatt ttttttcttt
    52381 acatttaagc tgatttcgct acaatgaatc tatattaaac attgtggcaa agtgaggtgt
    52441 agcaaaatgg aaatggaaca tattaatgaa aatacgatcc gtgtactaat tggtcatgaa
    52501 gatttagagg aacggggcgt ttcatttttg gatttacttg gcaatcacaa agaaatcgaa
    52561 aatttctttt atagtatttt ggaagaagtt gatatcgaag aggagttccg aggaagcgaa
    52621 gctgtcactt tccaagtttt gccaaaagga gacggattag aactgtttat cagtaaaaat
    52681 cttccggatg aagaaatttc tcaatcagaa gattctactg ttgattcaga agatgtgagt
    52741 gactttcttc gtcagcagat tattggcagc gatccggagg aagaattatc tggaagcgcc
    52801 ctagaagaac aggaaagaca agcaactttt gcttttgaag attttgaaca agtgatccaa
    52861 cttgcaacag atgtcgaact agagtctgct tggactgatt tgtatttatt cgaagagcgc
    52921 tattacctag tcgttcactt ctggatggaa aatctcaacc aagcggatgt agagaatcag
    52981 attgctcgaa ttttggagtt tgctgaaaag agcgatcgta caccagaaac attgaatgaa
    53041 tatggacaat gtctgatggc gcgtaatgct atcgagcgaa cgaaatttta ttttgtataa
    53101 atcagcatta tagtaagaga ctgtgacaaa aattgtgtcg cagtcttttt ttcgtataaa
    53161 tgtgttcaaa agcttgcatc tgcggtaaca agcccaacca aacgaaaaat attgggaact
    53221 atttttgttt agctgttctt attacttgat gcagaatagc tttttcacgc cctctttttt
    53281 cgtgtaaata aattcttaga cgtaaataag ttcagaggga gaaaaatgct gcttgcaatg
    53341 aatgaatata ataagtgctg ttttgctaaa catgcagaaa aaggaaagaa atattattgt
    53401 cgtggctgtc gaaaaagagt gattttgaaa aagggaaaga aaaaatgtgc tcattttgcc
    53461 catcaaaaat cagataactg ttcggtcttt agtgaaagag aatcaaaaga gcacttacag
    53521 ttaaaggaat gcttcatgga ttggctggga cagtcagtag aaccagtatt tttggaagcc
    53581 tatttgccga ggttgcggca aaggccggat attttgttgg cgaatctagc aatagaaatc
    53641 caatgttcta gactgtctca ccagagattt attgaaagaa cccaaaatta tttgaataac
    53701 agttaccaag tatggtggat acttggtaat tcttttcttg gacaatcgca attctcattg
    53761 atagaaaaaa gttgctgcta ctataataga aaaagaggag tgcattgctg gaaagctgat
    53821 ctcaagaaac aaaagcttta tttgtatcat catattacag agactgtatc agggcatatt
    53881 tccttttgca gtagctgctg gactttttct agtagggacc ttaaagaaat attcactagt
    53941 aacgaaatta aaatgaacca aatgaaaaaa atagaaaaag tgagtgaaga tgggaagaaa
    54001 tggctgacac gccaacttat tcacaagcaa aagaatacag tatccataca agaacaatgc
    54061 tacttgcgtc ataaacatct tttgcatcta tcatcgtgga tctatcaaaa aagtcggttt
    54121 ttcttttatt tacaagaaca ggtttttctt tatcgaatgt tgtacgaaga agccttggaa
    54181 aaccagaaag ttcctgattt caactcatgg ctttgccaaa taaaagaaca taaaagggaa
    54241 tggctttttc ctatgattga tgaagagatg gtctatcagc agttttttaa cgaatgcata
    54301 cacttgtctt ctttgaaaaa atagtaagta taaaaatagg ctaggaatcg gttttctatg
    54361 taaaacaaga tttagttatg ctaaaattga tggaagcaga cacatgaatg agtagaaaag
    54421 ttagatgctg tgacatgtgg atgttagact atgatctatt ttgatagata gagctaaggg
    54481 aagaaaagga gtggatttca tggaagtaaa gcagttgcca aaacgagaag aattgcctga
    54541 aaatttaact tgggacttga ccaagatctt ttcaagcgac caagagtttg atgagaaata
    54601 tttggaatta tcagaagagt taaaacaatc tgaaaaacac aaaggaacac ttgatcaagg
    54661 cgcttctcaa tttttaaatg cgattgaatt cgtattgagg gtttatcgcc aaactgaagt
    54721 catttatgta tatgcgcacc ttaaaaacga tcaagacact ggaaatacag attaccaagc
    54781 gctttatgca agagcaagca gtctgttttc gaaagttagt gaagccgttt cctggtttga
    54841 accagaaata ttgcaattgt cagatgacca gatttggcaa tatttcaaag aagaaccaaa
    54901 attggaagtc tatcgccatt atatccagca aatagtagat aatcgagccc atgtcttatc
    54961 tgctgagcag gaatctcttc ttgctggagc aggtgaaatc tttgatgctt caagtgatac
    55021 atttgctgtt ttgaataatg cagatctagt ttttccaacg attgaaggag aaaatggtga
    55081 aatagtccaa ttatctcatg gcgtgtatgg tcagttgcta gaaagcacgg atcgaagggt
    55141 gcgcgaagca gcatttaagg ggttgtacag tgtttacgaa caatttagaa atacatttgc
    55201 ttctacttta ggcacacata taaaaggaca taattttaaa gcgaaagtcc gtaattacag
    55261 ctctgccaga gaagcgtctt tgagcaataa tcatattcct gaaagtgtat acgatacttt
    55321 ggtagacgtg gtaaacaagc atttgccttt gttacatcga tacatggaat tacggaaacg
    55381 tttattagaa gtggaaaaac tgcacatgta tgatctttat acaccggtct taggggaagc
    55441 tccaattacc tttacgtacg aagaagcaaa agaaaaagct ttagaagcac tgaaaccaat
    55501 gggtgaagaa tacatggcca tcgtagaaaa agcattctct gaacgttgga tcgatgttgt
    55561 cgaaaataaa gggaaacgaa gcggtgctta ttcttcggga agctatgaca caaatccata
    55621 tattttattg aattggcatg atacgctgga tcagctattt acgcttgtcc acgaaatggg
    55681 acatagtgtt catagttatt tcactcgttc gaaccagcct tatgtgtacg gcgactactc
    55741 catcttttta gcagaaattg cttcgacaac gaatgaaaat atcctaacgg agtatttatt
    55801 ggaaacagaa aaagaccctc gtgtacgggc ttatgtactc aaccattact tggatgggtt
    55861 taaaggaaca gttttccgcc agacacaatt cgctgaattc gagcatttca tgcatacgga
    55921 agatgaaaaa ggtgtgccat tgactagtga atacctaagt gatagttatg gtaaattgaa
    55981 tgcaaaatat tatggtccag cagtcgaaga agacccagaa attaaattcg aatggtcacg
    56041 gataccgcat ttttattata attactatgt tttccaatac tcgactggct tttctgctgc
    56101 ttcagcactg gcgaagaaaa tactaaacca ggaaccagag gcactagaga actacttggc
    56161 ttacctaaaa gcaggtaaca gcgattatcc tgtggaagta atgaaaaaag caggagttga
    56221 tatgacacag gctgcatata ttgaagatgc aatgtcgatg tttgaacaac gtctaaatga
    56281 attagaagaa ttgatcgatc gtttataagg ctttctattt ttgattttct cgaatagaaa
    56341 cgaaaaaaat acgtcggctc tcttgtaagg gagtcgacgt attttttt
//
LOCUS       DACKAC010000012        51181 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.012, whole genome shotgun sequence.
ACCESSION   DACKAC010000012 DACKAC010000000
VERSION     DACKAC010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51181
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.012"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            118..972
                     /locus_tag="I0B46_000734"
     CDS             118..972
                     /locus_tag="I0B46_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAR2083056.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            973..2310
                     /locus_tag="I0B46_000735"
     CDS             973..2310
                     /locus_tag="I0B46_000735"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAR2083057.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            2315..2539
                     /locus_tag="I0B46_000736"
     CDS             2315..2539
                     /locus_tag="I0B46_000736"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAR2083058.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            2541..3431
                     /locus_tag="I0B46_000737"
     CDS             2541..3431
                     /locus_tag="I0B46_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAR2083059.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            3440..4258
                     /locus_tag="I0B46_000738"
     CDS             3440..4258
                     /locus_tag="I0B46_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAR2083060.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            4365..4814
                     /locus_tag="I0B46_000739"
     CDS             4365..4814
                     /locus_tag="I0B46_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAR2083061.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            4832..6514
                     /gene="recN"
                     /locus_tag="I0B46_000740"
     CDS             4832..6514
                     /gene="recN"
                     /locus_tag="I0B46_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAR2083062.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(6569..6685)
                     /locus_tag="I0B46_000741"
     CDS             complement(6569..6685)
                     /locus_tag="I0B46_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAR2083063.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(6758..7714)
                     /locus_tag="I0B46_000742"
     CDS             complement(6758..7714)
                     /locus_tag="I0B46_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAR2083064.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            7885..8253
                     /locus_tag="I0B46_000743"
     CDS             7885..8253
                     /locus_tag="I0B46_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAR2083065.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            8423..8854
                     /gene="mraZ"
                     /locus_tag="I0B46_000744"
     CDS             8423..8854
                     /gene="mraZ"
                     /locus_tag="I0B46_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAR2083066.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            8875..9834
                     /gene="rsmH"
                     /locus_tag="I0B46_000745"
     CDS             8875..9834
                     /gene="rsmH"
                     /locus_tag="I0B46_000745"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAR2083067.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIGLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            9839..10240
                     /gene="ftsL"
                     /locus_tag="I0B46_000746"
     CDS             9839..10240
                     /gene="ftsL"
                     /locus_tag="I0B46_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAR2083068.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            10241..12433
                     /locus_tag="I0B46_000747"
     CDS             10241..12433
                     /locus_tag="I0B46_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAR2083069.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            12459..13421
                     /locus_tag="I0B46_000748"
     CDS             12459..13421
                     /locus_tag="I0B46_000748"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAR2083070.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            13435..14796
                     /locus_tag="I0B46_000749"
     CDS             13435..14796
                     /locus_tag="I0B46_000749"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAR2083071.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            14822..15910
                     /gene="murG"
                     /locus_tag="I0B46_000750"
     CDS             14822..15910
                     /gene="murG"
                     /locus_tag="I0B46_000750"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAR2083072.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            15925..17145
                     /locus_tag="I0B46_000751"
     CDS             15925..17145
                     /locus_tag="I0B46_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAR2083073.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            17301..18626
                     /gene="ftsA"
                     /locus_tag="I0B46_000752"
     CDS             17301..18626
                     /gene="ftsA"
                     /locus_tag="I0B46_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAR2083074.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            18649..19890
                     /gene="ftsZ"
                     /locus_tag="I0B46_000753"
     CDS             18649..19890
                     /gene="ftsZ"
                     /locus_tag="I0B46_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAR2083075.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            19907..20584
                     /locus_tag="I0B46_000754"
     CDS             19907..20584
                     /locus_tag="I0B46_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAR2083076.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            20598..21197
                     /locus_tag="I0B46_000755"
     CDS             20598..21197
                     /locus_tag="I0B46_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAR2083077.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            21212..21499
                     /locus_tag="I0B46_000756"
     CDS             21212..21499
                     /locus_tag="I0B46_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAR2083078.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            21548..22330
                     /locus_tag="I0B46_000757"
     CDS             21548..22330
                     /locus_tag="I0B46_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAR2083079.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            22351..23058
                     /locus_tag="I0B46_000758"
     CDS             22351..23058
                     /locus_tag="I0B46_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAR2083080.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            23320..26118
                     /gene="ileS"
                     /locus_tag="I0B46_000759"
     CDS             23320..26118
                     /gene="ileS"
                     /locus_tag="I0B46_000759"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAR2083081.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(26167..27693)
                     /locus_tag="I0B46_000760"
     CDS             complement(26167..27693)
                     /locus_tag="I0B46_000760"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAR2083082.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(27708..28355)
                     /locus_tag="I0B46_000761"
     CDS             complement(27708..28355)
                     /locus_tag="I0B46_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAR2083083.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(28539..28868)
                     /locus_tag="I0B46_000762"
     CDS             complement(28539..28868)
                     /locus_tag="I0B46_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAR2083084.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            29045..29773
                     /gene="sfsA"
                     /locus_tag="I0B46_000763"
     CDS             29045..29773
                     /gene="sfsA"
                     /locus_tag="I0B46_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAR2083085.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            29789..30802
                     /locus_tag="I0B46_000764"
     CDS             29789..30802
                     /locus_tag="I0B46_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAR2083086.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            30802..32079
                     /locus_tag="I0B46_000765"
     CDS             30802..32079
                     /locus_tag="I0B46_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAR2083087.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            32142..34844
                     /locus_tag="I0B46_000766"
     CDS             32142..34844
                     /locus_tag="I0B46_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAR2083088.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            34996..35313
                     /locus_tag="I0B46_000767"
     CDS             34996..35313
                     /locus_tag="I0B46_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAR2083089.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            35343..35663
                     /locus_tag="I0B46_000768"
     CDS             35343..35663
                     /locus_tag="I0B46_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAR2083090.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            35749..37209
                     /locus_tag="I0B46_000769"
     CDS             35749..37209
                     /locus_tag="I0B46_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAR2083091.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            37277..37498
                     /locus_tag="I0B46_000770"
     CDS             37277..37498
                     /locus_tag="I0B46_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083092.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            37529..37711
                     /locus_tag="I0B46_000771"
     CDS             37529..37711
                     /locus_tag="I0B46_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAR2083093.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            37711..38124
                     /locus_tag="I0B46_000772"
     CDS             37711..38124
                     /locus_tag="I0B46_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAR2083094.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            38247..39428
                     /locus_tag="I0B46_000773"
     CDS             38247..39428
                     /locus_tag="I0B46_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAR2083095.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            39817..39890
                     /locus_tag="I0B46_000774"
     tRNA            39817..39890
                     /locus_tag="I0B46_000774"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39851..39853,aa:Arg,seq:cct)
     gene            complement(39959..41098)
                     /locus_tag="I0B46_000775"
     CDS             complement(39959..41098)
                     /locus_tag="I0B46_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAR2083096.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(41397..42032)
                     /locus_tag="I0B46_000776"
     CDS             complement(41397..42032)
                     /locus_tag="I0B46_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083097.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(42145..42780)
                     /locus_tag="I0B46_000777"
     CDS             complement(42145..42780)
                     /locus_tag="I0B46_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HAR2083098.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(42814..43275)
                     /locus_tag="I0B46_000778"
     CDS             complement(42814..43275)
                     /locus_tag="I0B46_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HAR2083099.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(43405..43836)
                     /locus_tag="I0B46_000779"
     CDS             complement(43405..43836)
                     /locus_tag="I0B46_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAR2083100.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDK
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(43854..44174)
                     /locus_tag="I0B46_000780"
     CDS             complement(43854..44174)
                     /locus_tag="I0B46_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAR2083101.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            44473..45249
                     /locus_tag="I0B46_000781"
     CDS             44473..45249
                     /locus_tag="I0B46_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAR2083102.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            45264..45467
                     /locus_tag="I0B46_000782"
     CDS             45264..45467
                     /locus_tag="I0B46_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAR2083103.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            45483..45821
                     /locus_tag="I0B46_000783"
     CDS             45483..45821
                     /locus_tag="I0B46_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HAR2083104.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            45808..45987
                     /locus_tag="I0B46_000784"
     CDS             45808..45987
                     /locus_tag="I0B46_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083105.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(46030..46500)
                     /locus_tag="I0B46_000785"
     CDS             complement(46030..46500)
                     /locus_tag="I0B46_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083106.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            46587..47285
                     /locus_tag="I0B46_000786"
     CDS             46587..47285
                     /locus_tag="I0B46_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAR2083107.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            47463..47804
                     /locus_tag="I0B46_000787"
     CDS             47463..47804
                     /locus_tag="I0B46_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083108.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            47797..48468
                     /locus_tag="I0B46_000788"
     CDS             47797..48468
                     /locus_tag="I0B46_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAR2083109.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            48474..49160
                     /locus_tag="I0B46_000789"
     CDS             48474..49160
                     /locus_tag="I0B46_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083110.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            49163..49912
                     /locus_tag="I0B46_000790"
     CDS             49163..49912
                     /locus_tag="I0B46_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAR2083111.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            49924..50193
                     /locus_tag="I0B46_000791"
     CDS             49924..50193
                     /locus_tag="I0B46_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083112.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            50355..50657
                     /locus_tag="I0B46_000792"
     CDS             50355..50657
                     /locus_tag="I0B46_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083113.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            50654..50815
                     /locus_tag="I0B46_000793"
     CDS             50654..50815
                     /locus_tag="I0B46_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAR2083114.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            50812..51117
                     /locus_tag="I0B46_000794"
     CDS             50812..51117
                     /locus_tag="I0B46_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083115.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
BASE COUNT        17247 a         8599 c        10632 g        14703 t
ORIGIN      
        1 gaattagaag ctggggcgta agtcctagct tttttctatg aatcatcata aacctgttat
       61 aaatcgggaa aattctatgg tagaatgaaa acacaaaaaa tatctgggag tggttagatg
      121 gcagaattga ttaacggaaa agaattagct gaaaaaatgc aggcagagat tgctgaaaaa
      181 atccaaaaat tgaaagataa tggtatccat cccggattag tcgttttatt ggtaggggaa
      241 aatccagcca gccaaatcta tgtaagaaat aaagaacgtg tagcaaaaga aatcggaatc
      301 cattcattgg tagagcgtta tcctgagacg atttctgaag acgaactact tgcagaaatt
      361 gaaaaataca atcaagatcc tagttttcat ggtatattgg tccaattacc attgccaaag
      421 catatagatg aagaaaagat cctgttagct atccgccctg aaaaagacgt agacggtttt
      481 catccgatga atttaggcag actccttgcg ggtaaaccgg acaagattcc ttgtactcca
      541 tacggcatca tgaaaatgtt tgaagcttat tctatcgatc cggcgggtaa acgagcagta
      601 gttatcggac gaagcaatat cgtcggtaaa ccgatggctc agctactctt gatggcagat
      661 gcaactgtta ctgttgctca ttctaaaacc gaaaatctgg ctgaactggc acaagaagca
      721 gatattttag tcgtagcaat aggacgtgga cattttgtca caaaagaatt cgtaaaacca
      781 ggtgctgtag tgatagatgt agggatgaat cgtgatgcta acggtaagct gatcggtgat
      841 gtgaaatttg atgaagtgga gcctattgca ggctggatca ctcctgtgcc aaagggtgta
      901 gggccaatga ctatcacgat gctgatgtat caaaccgtaa aatctgccga agcttttgga
      961 gcaggtgaat agatggcaca agaatattta actgtcacta cattaacgaa gtatttgaaa
     1021 agaaaatttg atgccgatcc ttatttagaa cgcgtatatt taacaggcga aatatcgaat
     1081 ttccgtttac gtgccaatgc ccatcaatat ttcagcttaa aagatgacca tgcaaaaata
     1141 tctgctatta tgttcaaagg agcattccaa aaattacgat tccagccaaa agaaggaatg
     1201 aaagtccttg tcgttgggcg tatttcatta tatgaagcgg gaggttctta tcaaatctat
     1261 gtagagcaca tggagcctga tggaataggt gctttatatc aagaattgga agaacgaaaa
     1321 gaaaagctga tgaaggaagg cctttttcaa gggcctaaaa aaatgctgcc acgtttccca
     1381 aagcgaatcg cggtccttac cagtccaagc ggagcggtta tccgcgatat catcacgacg
     1441 gtgaaaagaa gatatccaat tgtccaattg gttttgttcc caacagttgt acaaggaaac
     1501 caagcagcgg acaatgttgt caggaatatc cagagagtag aagagatggg caatttcgat
     1561 acaatgatca tcggtcgtgg gggtggttcg attgaggatt tatggccgtt taatgaagag
     1621 cgggttgctc gggcgattta tcaagctaat acgccggtga tttcatctgt aggacatgaa
     1681 acagatacca cgatcgctga tttagtagca gatgttcggg ctgccactcc aacagcagct
     1741 gcagaattag ctgttcctgt tttgtcagaa gagttgatgc gcattcaaga acggcaagct
     1801 agattgcagc aggcgtttgt acgccagatc cagcgcaaac aagaacggtt cgagcgtgct
     1861 cgacgttcct atgtatttcg gcagcctgaa agattgtatg aaggtcagac catcaaatta
     1921 gaccagttga aacagcgatt atatcaagct acacagcaaa tttatcatgc gaaagaaaaa
     1981 caaacgatcg cattaagcca ccaattggaa caagtggctc cgctttatcg agttaaagca
     2041 gctcgccaag aaactgctta tctagaaaaa cgcttagaag agaaaatcca acaatatatg
     2101 aaacaacagc agcagcgttt tgctcaagct gttcaatctc ttgatttgtt gagtccattg
     2161 aaaatcatgg gaagaggcta cagctatacg acgttaaatg atcaagtagt aaaaagtgtc
     2221 accgagattc atcaagggga tttgctgacg atccattatc aagatggaat cgttaaagga
     2281 caagtacaag agatagacaa aggagaataa aaatatggaa aatccaactt ttgaagagtc
     2341 actgcaagaa ttagaaaaaa ttgtcatgca gttagaacaa ggagacgttc cattagaatc
     2401 agctctagat tcgtttaaac gaggaatgga attaagcaaa cattgccaag atacattagc
     2461 taaagcagaa gagaccttga cgaaaatgat gacagaatca aatgaagaag ttgattttga
     2521 cggaaacgag gaaagctgag atgagattaa ctgacttttc agctattcat ctacctgctg
     2581 tagaaaaaga aatcttgtct tttttagatg agcatacaac ggatagagga ctctatgatg
     2641 cgatgagtta ttcagtgaaa gcaggcggga aaagaatacg tcccttatta cttttaacgg
     2701 ccgttgcttc ctttgatgag cctattgatg ttcccgtcta tcaagtagca gctgctttag
     2761 aaatggtgca tacgtattcc ttgatccacg atgatttgcc tgcgatggac aatgatgatc
     2821 ttcgtagagg aaaacctacc aatcataaag tattcggcga agcattagct atattggccg
     2881 gagatggttt gcttacagga gcattccagc taatcagtat ggcacactta ggtaattctc
     2941 ctaagcttct gctgcttcag caattagctg tttgtgcggg atcacaaggt atggttgctg
     3001 ggcaagctgc agatatcgag ggcgaatcga aaaagctttc tttagaagaa ttagcattta
     3061 ttcatgaaag aaagacaggg catttgattc gttatgcctt gctggctggt ggcatattag
     3121 cgaagcaacc tgaagaaatc ttattgctac ttcaacgatt agccgaacat ttaggtttag
     3181 cttttcagat aagagatgat ctactcgatg tgattggtac aacgaaaaca ttagggaaaa
     3241 cagctggtaa agatgaacgt atggaaaaaa atacttaccc gcgtttatta ggcctagaaa
     3301 agacacgtga ggcgttggag attgagctgt tatccgccaa caagatcata gacaaattag
     3361 aagaggacgt tttttctttt gatggcgggc tgatgcgcca gatgatcaag caatttgatg
     3421 tggagaaatg agggaaagaa tggaaaaaga acgcgtagat gtattagcgg tcaaacaagg
     3481 attatttgaa acaagagaac aagccaaacg ttcagtgatg gctggtttga tttacaacga
     3541 aaaaaacgag cggtttgata aaccgggaga aaaaattcca gtgagtagtg aattaagagt
     3601 caaaggtaaa aaacttcctt atgtttctcg agggggatta aaattagaaa aagcactcaa
     3661 agcatttgat ctatctgttg aaggaaaaac attgttagat attggtgcat ccactggcgg
     3721 atttactgat gcagctttgc aaaatggcgc aaaaatgagt tatgctttag acgttggcta
     3781 caatcagcta gcttggaaaa taagacaaga tcctagagtc gtcgtaatgg aaagagtgaa
     3841 ttttcgttat gcaaaacccg aagactttac cttaggaatt ccagaagttg ctgttatcga
     3901 tgtttctttt atttcactaa aactgatgct gcctccatta catgctattt taaaagaaga
     3961 gggagaagtg attgctttaa tcaaacccca attcgaagca ggcagagaag ctgtagggaa
     4021 aaacggaatt gttcgtgacc ctcagaccca tcaaaaagtt ttagaagaca ttttgtcatt
     4081 tgcggcacaa ggtggatatg atgtgctgga attaagttat tctccgatca cggggggaga
     4141 aggaaatatc gagttcttgg ctcacttgag gaaagtaccc gaaagcggaa caatcaattc
     4201 agctattaat atggcagaag tggtttcaaa tgcacacgaa cagtttgacc ataaataaat
     4261 tgtaactgtg aaaatcaagt gagaagtatg cggattcttc tttattatac caaggaaata
     4321 agctatcatt gattaagaag aatatgctaa ggagcgaaga cgtaatgaga aaaaaagatc
     4381 ggcatcgttt gattactcga ttgttgagtg agcaagatat ccgaaagcag gaagaatttg
     4441 ttgaaatcct gaaaagcaaa ggaatatctg ttactcaagc tacgatttcc agagatataa
     4501 aggaactgaa actgatcaag gtaccggctt cagatggcgg ctaccgttat agcataccag
     4561 ctgaaacaaa cgaagatgta aatactaaat tggaaaaact attaaaagac gctttcgtgg
     4621 cagttgacca aatggaaaaa tttgttattt tgaaaacatt gccgggaaat gcttcggcag
     4681 cagcaaattt gattgataaa cgttacaaga aagaattatt ttcgattatc aatgatgacg
     4741 ataatgtttt gatgattaca cgaactgaag accaggcagt tttattgaag aaagacttcc
     4801 tgcattacct ttaagttgag gagcgaatgt gatgctacaa gaaatcagta ttactaactt
     4861 tgcgattatt ccggaattgc ggctttcttt tcacgaagga atgaccgcgc tgaccggaga
     4921 gactggtgcg ggtaaatcca ttattatcga tgctttaggt ctgttagcag gaggaagagg
     4981 ttcaagcgat tatatacgtc aaggagcaga aaaatgtgtg cttgaagggt tgtttgaact
     5041 accaaaacaa gaaggattct ctgaactgat ggttgaatta gggatcgaaa cagatgagga
     5101 caatctgatt gttcgacggg atatgtcctt aactggaaaa aatgtttgtc gagtgaatgg
     5161 tcatattatc acattagcta atctgagaag aatcggcagt tatttagtag atatccaagg
     5221 acaaaacgaa catcaggaac tgctccagcc tgaatcacat cttgctttat tggatcgttt
     5281 tggagatgta gctttccaac agaagaaaaa gagttatcag caagaatata tctcttatcg
     5341 tgagctggaa aaaagggtac gaaaagtgca gcaaaatgaa aaatcctatg ttcagagaat
     5401 agatatgctc catttccagc aagaagaaat agctgctgct gatttgcaag tcggagaaga
     5461 agagaaatta aaagaagaaa gagaaaaact tagcaattat caaaaaattg ctgatggatt
     5521 agctgcaggt tacggagcac tgacgaataa tgaacaaaac agtgtagatg gtgttggctt
     5581 ggccgtttca gaactgcaag ggatcgccca tctagatgtg gagtatgaag caatttatga
     5641 aaatatccaa agtgcttact atttgctgca agatgcaatc ggcgatatga gtcgtcagat
     5701 tgatttattg gagttagatg agagtcgttt agaagaagtt acgcaacgtt tagaattaat
     5761 ccgacagttg aaacgtaaat acggcgaatc cattgagtca atattggctt actatgacga
     5821 aatcaccgaa gagctagctt cttctgattt ttcagaaggt caattggaca aaatgaaaga
     5881 ggaactagag caaaaagagc ttctacttca gcaacaagcc gcagatcttc atgaagcgcg
     5941 taaaaaaatc gcaaaagaat tggaaaaatc cattctgcat gaattgaaaa gtctgtatat
     6001 ggaaaacact gagtttgagg tacgtttctt aaaagaagaa aatagacaat tgaatcatga
     6061 tggatttgac caaatcgaat tttacattac gaccaatcca ggagagccac tgaagccttt
     6121 ggtgaaagtc gcttctggtg gcgaactgtc tcggatgttg cttgctttga aaactatttt
     6181 ttcttctgag caaggtgtga caagtattat ttttgatgaa gtggatacag gtgtgagcgg
     6241 aagagttgca caggccatcg cagataaaat ttctcaaata tcgaaatact ctcaagtttt
     6301 gtgcatcact catctgcctc aagttgctgc agtcgccgac tatcaatatt atattgtgaa
     6361 agcagtgatt gatggacgta cgcaaacttc tgtttcagag ttgaaaacaa aagaaagaga
     6421 agaagaaatt gcacgtatgc tggctggaag tgagattaca aaactcacac tcgaacatgc
     6481 aaaagaacta ttgaaactcg caaaaaggac ctgagccacc tcgcagtggt tcaggtcctt
     6541 ttcagcagct gatagctata ttttattttt acatgacact cattacagca gtcaatacaa
     6601 cagatccaat cgttaaaatc agcattgtcc aaatgacaaa ttttgttact ttagcaaaag
     6661 tacttgtttg ttttttttca cgcattgttt tcaacctcgt cattaattag ttgattcaag
     6721 tgtacaatct tttttccatt tccacaagag ggctttttta taaaaacttc ccttttttta
     6781 aataataggt agttacgata caaataaata atgttaatag aactagccat ataaaggcat
     6841 catccctttc agcaataggt aacttaatgt tcattccata gatactgccg acgattgtcg
     6901 gtatagttaa aataatcgtt aaagaagtta aaattttcat aatattattc agattattcg
     6961 atacgattgc tgaaaaagtc tgattcattt gttctactag ttttaattga atgcgagaag
     7021 tagtcattgc ctgctctgtt tccaccaata catcacgtaa atgatttttc aaagcatgat
     7081 tttcttggaa aagtttattt tgcgctagtt ctttcaatgc atctaaatta gcagaagtcg
     7141 cttctctgaa ataaacgagg cttttttgga tatccatgat ttgataaagt tgtttgtttt
     7201 cagtggatac ttgaagtttg gcttccaaag tatcgcattg agattgaagc gtagctaaat
     7261 aactgttgta agagagtgtg acctgccaaa tgaattgaaa gaagatattg agattaggcg
     7321 aaatatcact ttttggaaag gtctggctaa agactttctt taaaaacagt ggttcatgat
     7381 tcgtaattgt gataagcttc tttttgggcg tcaaaataat agagagcggg tatgtatcca
     7441 cttgaaaata accgctagga cttgttttag caaaaggata ttgtaacaat aataacgctg
     7501 gctttgtcaa ctttgattga ttgaattctt ctgtacggga attttctgca tcgtccaata
     7561 tggaagtgat gtaatctgac ggaatttcaa attgatgtgt cagctgatga atctcttctt
     7621 gattaggacg ttctacacaa taccagatcg tttggtcagt ttcctttccg gtattacgca
     7681 gtttctcatt atctaatgta aaatatttga tcatgatttt cctcctgttg cctgtttctt
     7741 ttattataac ggatagggaa ttggaataca tggaatgtgt ttgtaagata tagaatataa
     7801 ctgcataaat ttaaataaaa tgaaacagtt gtgtaaagta tatacagtat acttgaagca
     7861 acgataaaaa aggagtttac gttcatgggc acattcacac cacttatctt gttttggtat
     7921 atttacccgg taatcgtttt gtttggttgc caatttcttg taaaattatt ttcgttaaat
     7981 cgtcgtttta aaataaaagc ccctgatttg gcgattcctt ttttgtgggg cggtatgcat
     8041 gcattgtcta gaaatacagg gacaatttct atcttaccgt ttttctttat ttctgttttg
     8101 ctaatgggaa tttgtattgc tgttttccaa gcatattatt atgaagaaat tatttatccg
     8161 cgttatttca agatgacttg gcgtttggtt tttctgttga caatcgtctt gtacgctgtc
     8221 ttgatcattg tgaacatact ttcttaccta tgaaaaaaat agaaaagctg taaaaagtct
     8281 tttaaaaggc tttttgcagc ttgattttta aaaaaatgtt tatgaataaa aaaagttgtt
     8341 cccggatttc ccagaaaagg tggtagaaag tgggggattg tggtagacta attttagtta
     8401 gtggaggaat ggggggcttt caatgttgat gggtgaattc caacataata ttgatgcaaa
     8461 aggacgttta atcgtgccct caaaacttcg tgaagaactt ggcgaaaagt ttgttctgac
     8521 acgcggattg gatggctgtc ttttcggcta tccgatgagc gaatgggaaa atctggaagc
     8581 taaactgaac gaaatgccgt tggctaaaaa agacgcacgt acttttgtgc gattcttcta
     8641 ttcggctgcc actgaatgcg aacttgataa acaaggacga atcaatatac ccagcacgtt
     8701 aagaaattat gcagctttga caaaaggttg cgtagtcatc ggtgtatcta accgaattga
     8761 aatttgggat gagacacgat ggcaagattt ttctgcagca gcagaagaga actttgacga
     8821 aattgctgaa agtatgattg atttcggact ataagaatag gaagaggctg actaatgact
     8881 gaaacatttc aacactatac agtcatgttg aaagaaacgg tagatggatt gaacatcaaa
     8941 ccggatggcg tttatgtaga ctgtacactc ggcggtgccg gacatagtga atatttatta
     9001 agccaactaa gttctaaagg gcatctgtat gcgtttgacc aagatcaaaa agcaatcgat
     9061 cacgccaagc tgcgtttagc ttcttatgtc gaacaaggta tggtcacatt tatcaaagcg
     9121 aacttcagag aattagaaga acgcttgagt gagcatgtag ataaggtaga cggcattttg
     9181 tatgatctag gggtttcctc tcctcagcta gatgaagcag aacgtggatt tagttatcat
     9241 caagatgcac cgttggatat gcgcatggat cagtcagctc ctttatccgc ttatcacgta
     9301 attaatgaat attcctacca tgaattagta aagattttct tccgctatgg cgaggagaaa
     9361 ttttccaaac aaattgctcg tgaaatcgaa cgagtacgaa aaacaaaacc aatccaaaca
     9421 actggtgagc ttgtcgaaat cattaaacaa gtcatcccag ctcccgcaag aagaaaaggc
     9481 gggcatcctg caaaaagaat ttttcaagct gtgcggattg cagtaaatga cgaactcggt
     9541 gccgaagagg cctctttaga acaggcaatc gggttattga aagtcaatgg aagaatcagc
     9601 gtaatcacat tccattcatt ggaagacaga attgtcaaaa gtatgtttaa agaatatagt
     9661 acggtacaag atctaccacc gggacttcct gtcatacctg acgaattcca accagaactg
     9721 aaagtcatta ctcgtaaacc aatccttcct tcagaggaag agctaacaga aaacaatcga
     9781 tcacgcagtg caaagttacg tattgcagaa aaaataagaa tgaaagagga gtagaatgat
     9841 ggcagaatta aagaaaatgc aagattacga atatgattta cctgaattgg tggatcagcc
     9901 ggaacaacaa atgcctgatt cttcttcgaa tcaagaaatc attcttccac aatcaccggc
     9961 aaaacgatta aagcagattt ctgctttgga aaaaattgct gtggcaagcg tggcgattgc
    10021 tattgttgct ttatgtgttt taacagttat gctgcgcaca aatatcagcg gagctgaaaa
    10081 agaaatcacg tcaatacaaa cagaaatcaa tgataaaaag caagaaaaaa cgagtttaga
    10141 acaagaaaaa aacgaacttt ctcgtactga acggattaaa caaatcgcag aagaaaaagg
    10201 attgtctatt aatgacgaca atttaaggaa agtgaagtaa atgagtctta aaaataaaat
    10261 tcggcggttt atggaaaaga aaaatctaaa tccgatgaac aatcgcaaaa aagtaggtat
    10321 cattttattt gctacgagta ttggattatt ctttttattt gccgtccgtt tctcgtatat
    10381 cgttattgga ggtcatgttg ctggtacctc attagctgaa aaaacaaaac agttgtacca
    10441 aggaagtgaa gtagtaaaag caaaaagagg aacgatttat gatcgtaacg gtgtggcgct
    10501 cgcagaagat gcctcctctt attcaattaa agcgatactc tcaaaaacat atacctctgg
    10561 ggataaaaaa ctgtatgttg aagaaaagaa ttttgataaa atagcagaaa tcctgcataa
    10621 gaatctttct atagacaaaa aagatgcact gaatatttta gaggacggcg caaaaaaaga
    10681 gctgtatcaa gttgaatttg gtagctatgg gaaaaatatc tcccaagaaa ccaagcaaaa
    10741 catcgaagcg gacatgaaaa aagaaggtgt cgctggtctt tattttgtcg accatcaggc
    10801 tcggatgtat ccaaatggcg tcttttcttc tcattttatt ggatacgcag ttccagacaa
    10861 agatgagaat ggcttagttg gtaaactagg tttggagtct gcttacaatg atatcttgag
    10921 cggtaaagat ggtaagatta tttatcaaaa agataatttt caaaatcctc tgccaggtac
    10981 agtggcagaa gaagaaaaag ctgtcgacgg acaagatatc tacacgactt tagacagcag
    11041 actacaaagt tatttagaaa cactgatgga tcaagtaaat gaagaatatc agccagaaga
    11101 attgacagca gtcctgatga aagcaaaaac cggagaaatc ttggcaatgg gacaacgacc
    11161 tactttcaat cctgaaacaa tggaaggatt gactggaaaa gatgctatct ggcgtaattt
    11221 cttggtacaa gatagttatg aaccaggatc aaccatgaaa gttttcacta ctgcagctgc
    11281 tatcgaagaa ggagaattca acgagaatga aaccttccaa tctggaaaaa tccaagtaga
    11341 agatgctacg atcaatgacc atgactttgg tgaaaaaggt gtgctgacca tgcgtcaagc
    11401 tctttcatgg tcaagtaacg tcgggatggt catactggaa cagcgattag gcggacgatg
    11461 gtataactat ttacaaaaac tgggatttgg acaaagtact cattctggat tagatgatga
    11521 agtaaacggt gctttaccta cattaaatat tgtcgaccga gcaatgagtg cttatggaca
    11581 agcggtaggt gtgacgaatt tccaaatgat gaaagctttc acttctattg cgaataacgg
    11641 tacaatgatt caacctcgat atatcagcaa agtggtggat ccccaaactg gagaagagcg
    11701 aacaactcaa acagaagttc tagggcaacc tttttcaaaa gaaacaacag aaaaagtccg
    11761 tgaatatatg agagatgttg tcgaaagcga aaactacggt agtgcttatg gtgtatatag
    11821 tgtacctgga tacaatgtat cggcaaaaac cggaacagcc caaatcgctt ctgatacagg
    11881 gggatatcaa acaggagata cagcctacct gtattcgatt gtggagatgg ttccttctga
    11941 agatccagac tatgtcttgt atctaacaat gaaacaccct aagacatatg atcgaatggc
    12001 attggctaaa atcgcaaatc cattgatgaa acgggcaatg gattttaaag aaactgaaga
    12061 agattcagat acagaaacaa aaacagaaaa agtatccgta gccgattatc gtaatcttga
    12121 agcggacgtt gcagcagctg atgctcaaaa aagtggactg caacctgtag tgatcggtaa
    12181 tggtaaaaaa gtacaaaaac aatctactgc caacggtgat cagttgatct ctggagaaaa
    12241 actgatccta tatactggtg gtgataagct gatgcctgac gtaacaggat ggtcaaaagc
    12301 agatatcatg aagctgggga aaatcctcgg tattgaagtt agttttgatg gagatggtta
    12361 ttgtgtcaaa caagaacttg ctccatatga aaaaatcact gaagacaaac ttagttttac
    12421 tttagaagaa taagaagaga aataggagag aaaagagcat ggaatggaca caagcattga
    12481 ttcccattgt cagcagttgt gcaatgacaa ttgccgcaat gccgctattt attggttatt
    12541 ttcaaatgaa aaagcaaggt caggcgattc gtgaggaggg accaaagtgg cacaatagca
    12601 aagccggaac tcctacaatg ggcggcttag tgtttctgat cggctctatt ttgacaggaa
    12661 tttgggttgg agcttggcaa aaacagctaa ctcctactct tttcatcttg ctttttgtat
    12721 tagcgttata cggagtaatc ggctttcttg atgattttat aaaaatattc aagaaaagaa
    12781 acatgggact gaactcgaag cagaaattga ttggacagat tctaggggga atcatctttt
    12841 atcttgttta ccgttctgaa ggttatccag gtgtattgaa tttcttcgga ctagatctac
    12901 ctttaggaat catttatggt attttcgctc tcttctggct tgttggattt tcaaatgcag
    12961 taaatctgac agatggcatc gatggattag ttgctggctt aggaagtatc tcttttgcag
    13021 cttacggtct aattgcttgg catcagcatc agtatgatgt attaatcatt tgcttaagtg
    13081 ttttaggagg tttactgggt ttcttcgtct acaacagaaa acctgccaag atttttatgg
    13141 gagatgtcgg ttcattagcg ttgggcggac ttttagcggc aatttctata atgctaaacc
    13201 aagaatggac attgcttttg attggtttga tctatgttat ggaaacagca agtgtgatgc
    13261 ttcaagttac ttcctttaag cttacaggaa aacgaatttt taaaatgtct ccaattcatc
    13321 atcactttga aatgtgcgga tggtcggagt ggaagattga tacagttttt tggttgatca
    13381 gcattgtgac ttcgttgatc acattatgga tcgtttggta ggagatgacg ggaaatgaaa
    13441 aaaatatcaa catatgaaaa taagaaagtg cttgtcttag gactcgcaaa aagcggagta
    13501 agtgcggcca aattattaca tgaattgggc gcacttgtca ccgttaatga tggaaaacct
    13561 tttgacgaaa atccggaagc acaagagctg ctttctttag ggataaaagt aataacaggt
    13621 agtcatccaa tcgaattgtt ggatgaagaa ttttcattga tggtcaaaaa tccagggatt
    13681 ccctacagtc atcctttagt agcaaaagcc caagaaatgg gtattccggt aattacggaa
    13741 gttgagctag cttacgaagt agcggaatgc ccaattattg ggattacagg aacgaacgga
    13801 aaaacaacga caacaacgat gacaggacta ctactgaatg caggagcaga tcaaggaatt
    13861 gcacgcttag cgggtaatat tgggtatcca gcaagtggtg tagcgcaaga agcaaaatca
    13921 gaagataaaa ttgtgatgga attgtctagc ttccaactca tgggaatcac agatttccgc
    13981 cctcatatag cggtgattac caatatctat gaagcacata ttgattatca tggtacaaga
    14041 aaagaatatg tcaaagctaa atggaacctt caaaaaaaca tgacagaaaa agattatctg
    14101 atccttaact ggaatcagtc agaactacaa gaattagcgc aaagaacaaa agcacgcgta
    14161 ctgcctttct cgacaaaaga agtgttggaa gacggtgtgt atgctgacga ttactcaata
    14221 tactataaaa aagaaaaaat aatggaaata tctgaacttg gcgtaccagg taagcataat
    14281 gtagaaaatg cactagctgc tatttctgta gcaaaattgt atggtatatc aaatgaagct
    14341 atacgagaaa cgttgcattt tttccatggc gtaccacatc gtacgcaata tgtaggagaa
    14401 atccaaggca gaaaatttta taatgattca aaagcaacca atattttagc gactaaaatg
    14461 gctctaagtg gatttgaaac gtctaaggtc gtgttattgg ctggcggatt agacagagga
    14521 aatacatttg atgaattaat tccttcatta aaaggaatca aagcaatggt cgtatttggc
    14581 caaacaaaag aaaagttgat ggatgctgga aaaaaagcag gcatcgagac aatagtaaca
    14641 gccgattctg tagaacaagc agttccttta gcattagaaa acagtacaga tggtgatgta
    14701 gttttattat caccagcgaa tgccagctgg gatcaatacc caaattttga aactagagga
    14761 aacagattca tggaagctgt gaaccgatta aaatagaaaa agtagaagcg agcgataaaa
    14821 aatgaaaatt ttggttacag gcggaggaac agggggacat atttatccag ctcttgcctt
    14881 tgtcaattat gtaaagactc aagaaccgaa tgctgaattc atgtatgtgg gagcaaaacg
    14941 aggtctagaa aacaaaatcg tccctgatac aggaatgcct ttccatacct tggagattca
    15001 aggatttaaa cgaaaaattt ccatgcataa catcaagacg gtccgattgt ttcttaagag
    15061 tatccgtcaa gcaaaaaaaa tcctcagtga ttttcaacca gatatcgtta tcggtactgg
    15121 aggatatgtt tccggagcgg tagtgtacgc tgcagccaaa atgggtattc caacagttat
    15181 ccatgaacaa aacagtgtac caggtgttac caataaattt ttaagccgat atgtggatcg
    15241 gatcgctctt tcatttaaag atgcggcttc cttttttcct gaaaacaaag cagttcttgt
    15301 aggtaatcct cgagcacaag aagtagcaga tactaaaaaa tcagatattt tgaaaacatt
    15361 tgatttggat ccagaaaaga aaacagtctt gatttttggc ggtagtcaag gagcacttaa
    15421 aatcaatcaa gcagtatcag tttttttaag tgaatatatt ccttcttctt accaagtttt
    15481 gtatgcttca ggagatagat attatgaaga gatcctaaat aaaataaatc ctgaaataca
    15541 aaatattagt gtccagcctt atatcaaaaa tatggcagag gtcatggctt ccagtgatct
    15601 gctggtgggg cgtgctggtg caacttcgat cgcagaattc acagctctgg ggttaccggc
    15661 agtgttggtt cccagcccat atgttacgaa tgatcatcag actaaaaatg caatgagtct
    15721 tgttcacgca ggagcagcaa aaatgattgc agataatgaa ctaacaggtg aatcactaag
    15781 tcagacagtc aacgaaatca tgggtgatga agaattgcaa aagcaaatgt gccgagcttc
    15841 taaagaacaa ggaatccctg atgcttcaaa acgtctttat gacttagtaa aacaaataat
    15901 caaaaaataa aggcggtggt cttagtgagt aagaaaaaag aaccaaaaaa attgtctcat
    15961 ccgtcttctg atgaaaacaa tttgactcct tggcaaaagg ctaaccgaaa atatctggca
    16021 gaacaagaga tgaaaaacca agagccaaaa aaagaagaca taacattaga tacacccctt
    16081 tcagattctg aagaagaaaa aacggttcaa gaggaacagc aagaagaaag ccagtccgag
    16141 aaaaaaatcg aattttttga agagatcaag caaaagaaaa acggtggacc atataacggt
    16201 tcttttttga accggctgcc caatttaaaa agtcaacggc acaaagtact atttcgtcgg
    16261 ttagctttga tcatcactgt attgggtata ccgctgattt ttttgatcta ttatgtttct
    16321 ccttatagca aattacaagc agttgcagta tcaggcaata agacagtcaa cagccaagaa
    16381 atcatctctg atacaaaatt gtcattagga gaaaatgttt ggggacagta ttttcatcga
    16441 tctacttata tcgatcgcct taaaaaagca cagccaagaa tcgaaacagc aaatatacat
    16501 ttcaaaggga tgaatgaatt cgatttggat gtaacagaat ataaagagat tgctttgatc
    16561 gcaaaaaaca atcaatacta cccagtgatt gaaaatggaa cagtattaga tgaaaaagta
    16621 gcaaatccta caaaaaatct ccctatcttg gaagagttca aagactctgc aaaaatcaaa
    16681 gaattaacaa agcaatacaa tcagttatca tcagaattac aaaaagcaat ttctgaaatc
    16741 aagtacacgc caagagcaag caataaaaat ctgatccaat tgaatatgaa tgacggcaat
    16801 caagttattg taaatatcaa taatcttgcg aaccaaatga aatattattc gcaagtagcc
    16861 aaagatatgg atgaaaaagg tgtgatcgac atggaagttg gtatcttctc gcatccttac
    16921 ggtagcactc aagaaacgga agaatcgggc caatcggaag aaacaactga aagtgaagaa
    16981 aatgcggatc aaacaaacga atcaagtgaa gaggcagaaa cgcaaaaagc acaaacacct
    17041 ggatcgtcta gcacaaacgg atcatctgat tcaaatgatt caagtgaaac aagtcaaata
    17101 gaagataatc caagtacaac tccgtcaagt ttaaatgaaa attgataaaa caactttaaa
    17161 aagtaagaat gtactttcta aaaaccagta atttatggta gaattggaag cagtgaatat
    17221 atctattaat aagtatattt taaaacggac gctagttgtt cgcttgtata tggaatagta
    17281 agtaggaggg aatcccattc atggcaaaaa caggaatgta tgtaggcctt gatattggaa
    17341 cgacatctgt taaagttgtc gtggctgaat atatcgacag ccaaatgaat attattggag
    17401 taggaaacgc aaaatcagaa gggattaacc gaggcattat cgttgatatc gataaaacgg
    17461 tccaagcgat acaacgagca gtacgacaag cagaagaaaa agcaggtatc cagatcaaag
    17521 gcgtaagtgt cggtttgcca gcaaacatgc ttgaagtaga aaattgccag ggaatgattg
    17581 cagttaacgg agattcaaag gaaatcaccg atgaagatgt cagaaatgtg gcttcagctg
    17641 cccttgttcg ttcaatacct cctgaacgtc aaatcgtgtc aatattgcca caagatttta
    17701 ctgtcgatgg gttcgaggga atcaaagatc ctcgtggaat gatcggcgta agattagaaa
    17761 tgtacggtct gttgtttaca ggacctaaaa cgatcatcca taacatacgt aaatgcgtgg
    17821 aaaatgccgg attaatcgtc aacgaaatgg tgattactcc tttagcgctg actgaatcaa
    17881 ttctttctga cggagaaaaa gattttggta cgatcgtcat tgatatgggt ggcggccaaa
    17941 caacaacggc tgttatgcat gataagcaat tgaagtttac caatcttgac caagaaggtg
    18001 gcgaatttgt cactaaagat atctctattg tattaaatac gtcatttaac aatgcagaag
    18061 cgttgaaaat aaactacggg gatgcatacc cagaacggac atcaccagat gaagaatttc
    18121 cagtagatgt gattggtcag tcagaaccag tgaaagtcga tgaacgctac ttgtcagaaa
    18181 ttatttcagc tcgaatggaa cagattttca acaaagcaaa agatgcattg gaccaaatcg
    18241 aagcattaga gttgccaggt ggcgtcgtat tgacaggcgg agctgctagt cttcctggtg
    18301 ttgtggactt ggcacaagaa atcttcggcg taaatgtaaa attgtatgtt ccaaatcaca
    18361 tggggctgag aaacccggta tttacaaatg taataagtat cgtggattat tcagcaaact
    18421 tgagtgaagt gtatcaattg gcaaaaattg ctgttacggg tgaaacctca gcagctcgtc
    18481 aaatggtagt tgaacaagaa acaacaaaca catatgagag ctatgaagca cctgaagaaa
    18541 caatctacga tgagccggaa ccaaaagagt ccggagaaaa tgtaaaaaat aaaatcaagg
    18601 gtttctttac aaatattttt gattaattca tcctgggagg aataagatat ggaattctca
    18661 attgataata atatcaatga cggcgcagta atcaaagtca tcggagtcgg tggtggcggt
    18721 ggaaatgctg taaaccgaat gattgaagaa aacgttaaag gtgttgaatt cattacagcc
    18781 aacacagacg tgcaagcatt aaaaaattca aaagctgaaa cagttatcca attaggcccc
    18841 aaatatactc gtggtttagg tgctggttct cagccagaag ttggacaaaa agcggcagaa
    18901 gaaagtgaac aatccttacg cgaagcttta gacggtgcag atatgatttt catcactgca
    18961 ggaatgggtg gaggaaccgg tacaggtgct gcaccaatcg ttgcaggtat cgcgaaagaa
    19021 ttgggcgcat tgactgttgg tgtcgtgaca cgtccattca cttttgaagg accgaaacgt
    19081 ggccgttttg ctgcagaagg tatcgctcgt ttgaaagaaa atgtggatac attattaatt
    19141 atctcaaata accgtttatt agaagttgta gataaaaaga caccgatgct tgaagctttc
    19201 cgtgaagcag ataatgtttt acgacaaggt gtacaaggta tctctgattt gatcacagcg
    19261 ccaggttacg ttaaccttga ctttgctgac gtgaaaacag tgatggaaaa ccaaggtaca
    19321 gcattgatgg ggatcggtgt ggctagtgga gaagatcgcg tagtagaagc aacgaaaaaa
    19381 gcaatctcct ctccattgct tgaaacatct attgatggag ctgaacaagt acttcttaac
    19441 atcacaggcg gtttggatat gacattgttc gaagcacaag atgcttcaga tatcgtagca
    19501 aatgcagcaa ctggcgatgt aaatatcatt ctaggtacat ctatcaatga agaaatgggc
    19561 gatgaaatcc gtgtgacggt catcgcaaca ggtatcgatg aatcaaaaaa ggaacgcaag
    19621 tctagccgac cagctagaca agcacaaatg caatcacctg ctcaaaagac tgtattagat
    19681 atggatcaag ctaaaccaac ttcttcagaa gaagaaaaca gttttggcga ttgggatatc
    19741 agacgtgaac aaaatgttcg tcctagagtt gatgattcca attttgacaa tatcgaaaaa
    19801 aaagaatttg atacattcaa ccgcgaagaa acaaaatctc atggcgatga tgaattaaat
    19861 acgccaccgt ttttccgtcg taaaaaataa aggagaggaa gagcacatga ttgctgacaa
    19921 cttgcagaaa atcaagcaag aaattcagga ttcgtgtgct cttgttcatc gtgatccgaa
    19981 agaagttaca ttagtagctg taacaaaatc tgttgaatcc gctcaagctg aagaattggt
    20041 tagagctggc gttatgaatt gtgcagaaaa ccgagtagat aaattattag aaaagaaaca
    20101 agatttattt gattttcctg aaataaagtg gcatttaatt ggtaatttac agcgcagaaa
    20161 ggtaaaattg attgtaaatg aaattgatta ctttcatgcg ttggacagtt taagattagc
    20221 agaagaaatc cagaagcggt tagagaaaga attatcctgt tttattgaag taaatgtttc
    20281 tggtgaggaa tcaaaacatg gaatttcacc agatgagctt ttaccgtttg tggaatcttt
    20341 agctgcctgc acaaaaatca aggttgttgg gttgatgact atggcgccta aagaagcttc
    20401 tgatgagcaa atccataaag tgtttggtac gctgaaagaa ttgcgtgatc ttgttcaagc
    20461 aaaaggttat gcccatgcac catgtagtga attaagtatg gggatgagtc aagactttca
    20521 aattgcgatt gaagaaggtg caacatttat ccgtgtagga acagcgctat tcaaagacga
    20581 ataaaggagg aacaaaaatg ccgcttttaa acagaggagc aattgctaat tttttcggat
    20641 tagctgatga ggaagagtac gaataccaag attatcctga aacaacaaaa aaggctgtac
    20701 agccaactaa aaaaaataca acacagaatc aatcaactca ttctgtatct gctgcgaata
    20761 ctcatgaaaa acaaaaaacg gcgacttcac gtcctgtaac gcatgaaagt attcagccgg
    20821 ctgaaaagaa aaaagtaatt gaactacatc aatcacctac aaaaactgta gcgaaagaaa
    20881 gaacagcttc aagaacctct acacaaccta aagcaaatct tgctggaaaa atcactgtaa
    20941 tggaaccaag agcttattct gaagctatgg cgattgctaa acgaatcatt gctggagaag
    21001 caaccttagt gaattttcat ttagtagaag aaaaacaagc gagaagaatc gttgattttc
    21061 taacaggaac agtttatgca ctagatggag atatcaaacg tgttggagat gagatttttc
    21121 tttgtacacc gactgggatg gagattgaca gttcaaccgc tcaatctttt gcagatgcga
    21181 atttgtttga tctgtaagag gaggaactac gatttatact attgcactat tacttcattt
    21241 acttcaacgg gcagcctatt tctatagcat tatcttgatg gtatatgcat tgctctcttg
    21301 gtttccaggc gggtatcaat cagggctagg acgattatta gctaagatct gcgaaccgta
    21361 tatcagtttg ttcgatcgtc tgcctttgag gattggaccc ttggatttca caattttagg
    21421 ggcaatcctg atcttgaact tagccgtaca aggtttagca cagatcgtta tatggataac
    21481 tgtccattta gtttattgat tgaagcaaca aaatctttat tctcagaata gataggaggg
    21541 gaaaattttg gatgcaaatg tataccagca ctttcgggca gatgaacgta catttatcga
    21601 ttcggtaggc gattggattg aacaggttca aggacaatat gcaccctatc tgaccgaatt
    21661 tcttgaccca agacaatcct atattttaga aacactgatt cgacaaagca gtgatttgcg
    21721 tttcatgttt tatggcggat atgaacaggc agaaagaaaa agatgtttga ttttccccga
    21781 ttattacgaa cctgtagagg aagattttga aacagcattg tatgaaatcc attatcctag
    21841 caaatttgcc agcctaagtc atgggaaaat ccttggaact ttagtgggaa cggggatcaa
    21901 acgctcttac tttggcgata tcatctcgga cggtgatcgc tggcaagtat ttattgcaaa
    21961 tgaggtggat cattttgttg ctgcacaagt aacaaagatt ggaaaggttg ctgtacgctt
    22021 ggaaccaatc aaatacacag aaatgattat gccgaaagat agctggacac aagaaagagg
    22081 aacagttagt tccatgcgac tagacagtgt gatctctgag atttataata tttccagaca
    22141 gcggtccaag cagctgatcg aagctggtaa agtaaaagtg aactggacag aaaatacaag
    22201 acctgatttt gtacttgaac tattagatat tatttcagtc agaggttatg gcagagtcca
    22261 gatccaagaa cttgagggga agacgaaaaa agaaaagtac agggtactgt tcggtgtatt
    22321 acgtaaataa aataaaaagg tggtttactc atggctttaa ctccattaga tatccaaaat
    22381 aagacattcc cgacaaaaat gcggggatat aatcaagacg aagtagatga tttcttagat
    22441 ttagttgttc gtgactatga ggaattgact caaagaaatc gagaattaga aaaagcagtc
    22501 aaacattctg aagaaaaact tgagtacttc aatgaattaa aagatgcttt gaaccaatca
    22561 atcatcgtag ctcaagatac ggctgataaa gtaaaaacaa gtgcaagcaa ggaatccgaa
    22621 gtgatcgtta cttctgcaca aaacaaagca gatgagttag ttgcgaacgc tgaaaaacgt
    22681 gcacatcaat taacaactga tgcagaagaa aaagcaagaa aaatcttgac agatgcaaca
    22741 gaaaaagcaa gacagcttgc gacagaaact gaagatctta agaaaaaaac acgagtattc
    22801 catcaacgta tcagcttgat gcttgagtct caattagaac aagtgaaaag tccggaatgg
    22861 gatgaaattt tacaaccatt ctccagctac gtgactgaca gccatgaagt gatcaaagaa
    22921 gttttagcaa aacagcttga caatgaaaat gatactgatg taaactcaga cacaggagtc
    22981 atagaagcac ctatgacggc atttgataca caggctattg atatctcgat cgtgcctggt
    23041 gaaaatgaaa acgagtagaa cagaaaagca ctttgttatt cttaaagcga gccgatgaga
    23101 gtggaagttc ggtaagacac tgcatggtga aagatcctct acttgagacc tgcttttccg
    23161 aaattagagt aagaagcagc ggctgacctc gttattggtc acagagagtt tgcactagat
    23221 agatgcaact aaatttgggt ggtaccacgg catgttcgtc ccttgcggct gaacatgctt
    23281 ttttgtgtcc ggaaaacaaa taaaggagtg tatggttgaa tgaaaatgaa agaaaccttg
    23341 caattaggaa aaacaaaatt ccctatgcgt ggtaatttgc caaaccgaga aggcgagtgg
    23401 caaaaagaat gggaagaaaa tcgaatctac caaaaaagac aagagttgaa cgaaggaaaa
    23461 cctagctttg ttcttcatga tggacctcca tatgccaatg gtaatatcca tttaggacat
    23521 tcattgaata aaatcagtaa agacattatc attcgttcaa aatcaatgtc tggctatcgt
    23581 gcaccatatg ttccaggatg ggatacacat ggattgccga ttgaacaagt attggctaat
    23641 aaaggcgtga aacgtaaaga aatgtccatg gcagaatact tggaaaaatg tcgtgaatat
    23701 gcactttccc aagtagataa gcaacgagca gatttcaaac gcctaggagt tgcaggagat
    23761 tgggaacatc cttatgttac attagatcct tcttatgaag ctgcacaagt acgcgtattt
    23821 ggtaaaatgg ctgaaaaagg ctatatttac aaaggattga aaccaattta ctggtctccg
    23881 tcaagtgagt cttctttagc ggaagctgaa attgaatata aagatgtaaa atctccatct
    23941 atttttgtag ctttcaatgt caaagacggc aaagatattc ttgatgaaga tacagctttt
    24001 gtcatttgga cgacaacacc atggacattg ccagcaaacc aaggtattgc tgtaaatcca
    24061 aaatttacgt atgtattggt ggaagccgat ggccgtaagt ttgtcgtagc gaaagatttg
    24121 ctagaaacag tccaatcaga aatcggttgg gaaaatgtaa cagttctaaa agaatttgct
    24181 ggacaagaaa tggaatatat gactgcaagt catccattct atgaacgtga atcacttgtg
    24241 attttaggcg atcatgttac tttagacgct ggtactggat tagtccatac tgctccagga
    24301 catggggaag atgactatat cgctggtagc aaatataaac tatctgtagt gtcacctgta
    24361 gactcaaaag gattgtttac agaagaagca cctggatttg aaggtatctt ttatgacaaa
    24421 gcaaatccta tgatcactga tctattaaaa gaaaaaggag ctttgctgaa gctagatttc
    24481 tttactcata gttatcctca cgattggcgg acgaaaaagc ctgtgatcta tcgggcaact
    24541 ccacaatggt ttgcatctat cgataaattc cgtcagaata ttttggatga agtagaaaaa
    24601 gtagagtggg ttatcccatg gggtaaaaca cgtttgtata atatgatccg tgaccgcggc
    24661 gattgggtaa tttcccgtca gcgtgcatgg ggagtaccac ttccgatttt ctatgctgaa
    24721 aatggagaag caatcatcac accagaaaca atcgaacatg tggcaaaatt atttgctgaa
    24781 catggatcaa ttatctggtt tgaaaaagaa gcgaaagaac ttcttcctga aggatttacc
    24841 cacccaggtt ctcctaatgg cgaatttaca aaagaaaaag atatcatgga tgtctggttt
    24901 gattctggtt cttctcatga agctgtttta cgtcagcgac cagaattatc attcccagca
    24961 gatatgtatt tagaaggatc agaccaatat cgtggctggt tcaactcaag tatcacgact
    25021 agtgtggcta tcaatggtgt agcaccatac aaatcagtta tttctcaagg atttgcttta
    25081 gatggtgaag gccgtaagat gagtaaatct ttaggaaaca tcattgcacc agaaaaagtg
    25141 atcaaacaat ttggtgcaga tatccttcgc ttatgggtat ctagtgtaga ttatgaagca
    25201 gatgttcgtg tttctatgga tatcttaagt caagtggcag aagtatatcg taaaatcaga
    25261 aatacgatgc gtttcttatt agcgaataca gaagatttcg atcctaaaaa agatactgtt
    25321 tcgtacaatg atttacgttc tgtagacaaa tatatgaccg ttcgattgaa ccaagtaatc
    25381 aaagaaatcc gagaagaagg gtacgacaaa tataacttca tgcatattta ccgtacagtt
    25441 atgaacttct tgacggttga tctatcgtca ttctacctgg attttgctaa agatgtagtt
    25501 tatattgaag cagaagatga ttatcaacgt cgttgcatgc aaactgtttt ctatcaaaca
    25561 gctgtagctt tgacaaaatt attgactcct atcattccgc atacagcaga agaaatctgg
    25621 tcattcctga aagaagaaga ggagtacgtt cagttaagcg agtttcctgg ttatgaagag
    25681 tttgcaaacc aagaagaatt gatggatacc tggactgctt tcatggactt ccgtgataat
    25741 gtcttaaaag cattggaaga agcaagaaac agcaaattga ttggtaaatc tttggaagca
    25801 aaactaactg tttatccaaa acaacaagtt cgtgaaatgt taaaagcttt ggatgcagat
    25861 attgcacaat tattgatcgt ttcaccagac tactttgaag ttatggcagc agatgaacaa
    25921 gtgccagata acgctatggt atttgatgat gttgctatta cagtggaaaa agcagacggg
    25981 gaaacttgcg atcgctgtcg ccaagttcgt aaagatgtag gagtagatga aaaacttcct
    26041 catctatgcg gtcgttgtgc aaaaatcgtt gaagacttct atcctgaagc tgtagctgaa
    26101 ggattcgaag aaaagtaaaa gggaaaaggg ggtgtgacat gttctttgtc acaccccctt
    26161 ttgttatcaa tcgtttaatt ttccacgttc acgataccag ttatctggtt gccagatcca
    26221 ttcgaatccg tctttttcaa gtagttcgaa agctgcttta ggtcccattg atccagcttt
    26281 gtagttggga aaatccggtt gtgtttggtc ccaagctttt cgaatgacat ccacgatatg
    26341 ccaagattga gcgacttctt cccaatgaga gaagttcgtt ccatcaccat tcaacgcatc
    26401 taatagtaat ttttcgtaag cttctgggct attttcaacc atttctgcac tatttctaaa
    26461 ttccaatttg attggttccg tttcaaatcc ttgccctact tctttcccat tcaaggataa
    26521 agaaaatcct tcagttggct gaatatagat cgttagaaca ttaggtggaa gtgtcgtatc
    26581 gtcacatggt tcatcgacag atgttttgaa tacattaaca ggaacttgtt tgaatacgat
    26641 gttgatccga gtcccttttt cagtcaaacg tttgcctgta cgtacataga aaggaacacc
    26701 tgaccaacgg aagttatcga tagtgaattt gcctgcgacg aatgtttcag tctctgattg
    26761 agcatccact ttgtcttctt cgcgatagcc cttgaattgt acaccatcca attcaccttg
    26821 agcatattgc ccgcgaacga agttttctaa cacttcttct tcgttataaa tgcggatagc
    26881 tttcaatgct ttgatctttt ctgtacggat ttctttttcg gaaaatgcta ctggcggttc
    26941 cattgcaagt aatgctacaa cctgtaaaat atgattttgc accatgtctt taagtgcgcc
    27001 actatgatcg tagtagcccc cgcgatcttc tacaccgagg ttttcgccaa aagtaatttg
    27061 gatattgtcg atatagcgat tgttccattg agcctcaaaa atattattgg caaaacggat
    27121 agctgagatg ttttggatca tttcttttcc taaataatga tcaatcctga aaatatcttg
    27181 ttctgggaat acttctctga tttgctggtt caattcaaaa gcagactggt aatcagaacc
    27241 gaacggtttt tctatgatca aacgattgaa gccatttttt gtcataatat tttgtgattt
    27301 caaatgagaa acgatcgttc caaaaaattg aggtgccata gccaaataat agacacggtt
    27361 accgtttaat tgatagtgtc catctaatcg gtcagacaat tcttttaatg tctgataatg
    27421 cgttgaatca tttacatcat gggattgata gtaaaaatgg cttgcaaagg tagttgcttc
    27481 ttcatctgaa ggattcaatg cacggattgt ttctttgaca acttcacggt agtgctcatc
    27541 gctccaggga cggcgagcgg ttccgattac agcaaaatgc tcgcctaaat ttccttttct
    27601 gtaaaggcgg aacaaagacg ggtacaattt acgctgggct aggtcacctg taccgccgaa
    27661 tatcgtaaac aatacatttt tttcattaga catgtggata cactccttta tattttatcg
    27721 acaaaaatcg tgctggccgc cttaaagctg acggggattt tctttctatc attttggata
    27781 gtgatcggtc cttcataagc tgcgatgtca acgatctcat attcttcgtt gatattcagg
    27841 tcaatagtga ctaagtaatc caacaattct ttttcatcaa gcactcgtgc gatacgcact
    27901 tttgtaccga caggatagga ctccaatgtt tgtcggcgtt cttcatggac aacttcctct
    27961 tttttaggaa tcgatcctcc atgaggacaa tgttcaggat gattcaaata gttttctaaa
    28021 tgatctgcaa gatgttccga agtcacatgt tccaatactt cagcatcatc atggacttca
    28081 ttccagctat agtctaaatg ttcaactaaa aaaacttccc ataagcgatg tttgcgaatc
    28141 agactgctag ctttttgcag tccgcttgca gtcaattgta caccttgata aggggaatgt
    28201 tctacaagct cttctttcac aagtttagaa atcatttcac tgacagatgc agctgagaca
    28261 tctagactag atacgatttg tttattgttg actttcgttg tatctccacc tagttctaaa
    28321 atgattttca aataatcttc tcgatttggt gtcatattcg atcatccttt catggtaaaa
    28381 aaccatcatt agtaagtgta acaaaaaaaa cgttaattga ctatttttct aaataaaaaa
    28441 caaaggattt ttagacgttt ttacaacaaa aaacgaaaga tgatagagaa gactcattct
    28501 ttcgtttata aaggatatat atatacattg ggagggatct atttttcaga taacaactta
    28561 tatgaagtgt ctgcagattc gtcataaaga gtttgttcgg gataagaaaa gataagataa
    28621 aaaccatact gatcacgtac gactaaacga tattcatcta tttctaccac agatgtgcca
    28681 agagactgac cgttgatcaa aaacgtgtaa tcagatgtaa tctgtaattg ttgtttttgc
    28741 tggctatcac taaagaacca tgtaccttga tactttgaga gtccttctga cttttccgct
    28801 tgttttttct ttttcaagta aataaaactt gagagaacgg taatgagtga accaagaaaa
    28861 tagatcattt tttttatcac caaatcactc ctttatgaga aaataatcgc atagacatat
    28921 tctttcttct ttatttcatt gtaccgaata aaagctaaaa tttctctaag actttcgact
    28981 ggttttttta ctgtttttct acaaattatg ataggataca tagtaggaag aaaggtgaca
    29041 taatttgaag tatcagcaag tggaaatagc tttttttatt gaccgcccga atcggtttat
    29101 agcacattgt atgaaaaaag acggagaaat aattattgcg catgtcaaaa atacaggtag
    29161 aggaaaagag gtcctgatac caggcgctga agtggctatt gtatttgctc ctggacctaa
    29221 acggaaaacc gcctatgatc tgattgctgt aaaaaagaag ggtgactggt ttaatatcga
    29281 tagtcaattg cctaatcggc tggcaattga tggtatttta gacggcacga tccttttacc
    29341 aggattaact ggtccattta cggtatacaa gcgtgaagta acttatcttc actccaaatt
    29401 tgatatttat ttagagacgg tggtcggaca aaaagcattt gttgaagtca aaggtatgac
    29461 acttgaaaat aaagcgattg gtgcttttcc tgatgcgccc acactcagag gattgaaaca
    29521 tataggagaa ttaacgtatg ccgcacaaga tggttatgcc gcttatgtct tgtttattgc
    29581 tcagttcgaa catcttcatc tggcaacgat ccatgaagaa atgcaaccag cactagctga
    29641 tatggttcga catgcgcagc aaagcggagt tcaaatcctc gcctataatt gtcaagtatc
    29701 ggcagatgag gtaactgtca agcaggccat tccatttgat gtagatatcc cttttgaaga
    29761 tcctatacaa tagaaagaag gtaaaataat gatcaactta ggtattatcg gaacaaattg
    29821 gattactcat caatttgtcc aagcagcttt aagcacaaaa aaatatgaat tgacagcggt
    29881 atattctaga catttggaaa aagcacagga atttggttct cattatgaag gggatatcga
    29941 attcgcaaca gatttgaaga cttttttcaa tattactcat atggacacca tttatattgc
    30001 ttctccgaat agtcttcact ttagtcaagc taagcaagca attttggcag gaaaaaatgt
    30061 gatagttgaa aagccagctt tttcgacgcc aaaagaaatg gatgaaatca tccatttggc
    30121 aaatgaacac aatgtgtatt tttttgaagc agcaagaaat atccatgaat caggttttaa
    30181 aaagattgcc gattacttac cattaaaaga cgagataatc ggtgcaaatt tcagctatat
    30241 gaaatattct tcgcgctatg accaagtatt agatggagaa gaaccgaata tcttctcgac
    30301 tcatttctca ggtggtgcat tgatggattt aggtgtttac cttgtatatg cagctgttgc
    30361 ttggtttgga atgccaaaag aagtccatta tttcccaaga aagatttcta ctggtgtaga
    30421 tggtcttgga tgggggatct tacgttatga gaattttgat gtatcgatcc agccaggtaa
    30481 aatcggtgat tcttatttac cgtcagagat ttattttgac tcaggaacgc tagtgatgaa
    30541 tggagtcaat gcaattgaac aagcacaatt tcatgatcgc gcgcataaag aaatcattga
    30601 tttggcggta acagctgcag aaaatccaat ggcagatgaa gcagaggatt ttgccaatgt
    30661 catggctcat ccgaaagatc cagcttgggg attgttgtat gaagagtgga tcgagctagc
    30721 tagaaatgtg aaccaagtga tttatgattt aagaaaaaat ggcggcatca aattcgatgc
    30781 agataatgag gaagattttt aatgaacgaa tttgtacaag tatttcccga aaattggcag
    30841 aaaaaatatg aggataaagg gtttatctca ccttctctta ttcaacgagc agtttttcag
    30901 ccactaagta ataaacaaag tatagtagcg gtttctccta caggttcagg taaaacactg
    30961 gcttatcttt ggcctctact actaaacgtg gaaccaggtg aagcaagtgc gttggtcatt
    31021 tttgcttctt ctcaagaact agctattcaa gtagcagatg ttgcacgtga atggggaaaa
    31081 gacaaagagt tgaaagtcca atctttggtg ggcggagcca acgtcaaacg tcagattgag
    31141 ggtttgaagg aaaaaccaga aatcttgatt ggtacaccag gaagaatatt ggaattgatg
    31201 aaagcaaaaa aaataaaagc acatcaagtg aaaacaatgg tatttgatga agcagaccag
    31261 ctatttgacg ctgggaacag ccaaatcatt gatcaaattc ttcatcaagc tccaacagag
    31321 tatcagctag cttttttttc agcgacagca gatcgttccc ttgaaagcat tgaaaaaatc
    31381 actgggaaaa cgtttgagac aattgacgtg acagcagagg acgattctcg aaaaggactg
    31441 agacattact ttttacgtgt accgatacga aagaaagatg actacttgag acgtctcacg
    31501 catatagaag attttcatgg tctgattttc tttaatcaac tgactgaatt aggcgtaatg
    31561 gaagaaaaat tgctgtatag aggtgtaccg gtaggaagtc tggcctctga tcagaacaag
    31621 ctactccgaa aagcagcgct agaacaattc aaaaagaaaa tggtcagagc acttttgacc
    31681 acagatgtgg cggctagagg tttagatatt acaggtcttc cttatgtagt gaatgcagaa
    31741 gttccgttaa gtgaagaagc ttatttgcat cgttcgggaa gaactggtcg aatgggtaat
    31801 gaaggaatag taattacact cgtacaagat aataatctaa gagatttaaa acggctttta
    31861 cgtccatcaa atataacact tgaagagatt tttttatacg gaggtaagct gcaaactgag
    31921 cagccaacca aagaagatgc agctcctgca gtaaataaaa agtatgcgaa aactttttct
    31981 ggaaaggagc agaacccaga atataacaaa gaaaaaataa gaaaaaaagt taaaaataaa
    32041 tcaaaaaaag agagaaataa aggagcaagg cgcaaataag cgccttttta tgctatgatt
    32101 atgaaaacgg gaacaaaatt ggttgccaga ggaggggtta tgtgaaagaa cgtcaagaag
    32161 agatcttgca gctattgaca gaaaaccaac aaggattgac agcaagtgat gtagctgagc
    32221 gcttaactat cgatcgaagc aatgcaagcc gatatttaag tgaactatac aaggcccatc
    32281 atattgtaaa aaccgctggc cgaccagtcg tttactcttt gccaacggaa aagtcaaaat
    32341 ctgatgaagt gcatgtcgac tcttctacgc aagtgacttt tgaaacactc gttggagaaa
    32401 atgattcttt aaaagtcagc atacagcagg caaaagctgc gattctttat ccaccgcggg
    32461 ggttgcatac gattattttt ggagaaactg gtacagggaa gtcgatgttt gctgaatgta
    32521 tgtatcattt tgcaatcgat tcagagatgc tttctgcaga tgcgccattt gtttctttca
    32581 attgtgcgga ttatgctcaa aatccgcaac tgctctttgg acacatcttt gggatcaaaa
    32641 aaggggctta cacaggagca gctcaagata gtcccggtct aatagccaaa gcagacggcg
    32701 gtatattgtt tttagatgag atccaccgtt taccgccgga agggcaggaa atgctgttta
    32761 ctttcattga taaaggtatt taccgtccgc ttggagaaag tgcacaagta catgaagcat
    32821 ctgttcagat cattggggcg acgactgaat cttcagaaag tttcttatct acgttcaatc
    32881 gacggattcc tatggcaatc accttgccga atcttgcttc acgttcactg gatgagcgtt
    32941 atgaaatcat ctctcttttt atcaaacaag aagcgaatcg tttgaatcaa cgtatcgata
    33001 tcgaaaaaga agcgattctt gccttcatgc tttatgatgc ggaaggaaat atcggtcaag
    33061 tgaaacgaga cttgaaacta gtctgtgcaa aatctttttt acattaccgt actcatcatg
    33121 agaaaaagct aatcattcga aaagaagaac tttcccttca agtacaaaaa ggattactga
    33181 aaatcaagga aataccggaa cgtctcgacc gattcatgga cagcaaaagt cagtatttga
    33241 catttgaacc aggatttgct gatgttgtct ggtcacaaga tccagaacga aacatgcagg
    33301 tctataacga tattgaggaa aaagtcactt ctctttccga aaccggtgtg gagaacattg
    33361 atctagagac cctcatttca aaagatgtgg atgcttattt ccagacatat gtcaaagaat
    33421 tgactaaaag ttcgattccc aaagatcttt tgccagatga aatctggcgg ttaaccaatc
    33481 aattgtatga tgtggctgaa aaacggttag accgtattta taatgaaaaa gctcgttttg
    33541 cttttgcttt gcatctgcaa agtacattgg atcgtgtgag agaaggtcat atgatcgtcc
    33601 acccagattt gaataacgta cggaaaaatt ttaagaggga attccaggta gcaatcgatc
    33661 tttcaacgat cattgaagaa gaagaacaag ttgagattcc ttttgatgaa atcggcttta
    33721 ttagtatgtt tctttctatc aatctaggag aaagtgagcc tgttaaagaa aacaaagtag
    33781 atgttctcat attgatgcac ggcagacaga ctgcttcaag catgttggag atggcacaag
    33841 aactattagg aacaaaagta gggaaagctt ttaatatgcc actggttatg gaagttcaaa
    33901 caatgtatga agaagtgtta caatatgtca aaaactatca agagcatttg acgaatggcc
    33961 tactcttgtt aacagatatg ggatcattaa acaccttcag tgatttgatt tatgaagaga
    34021 ctggcattcg gacgaaagct atcacgatga caagtaccat gattgtgata gaagcgatac
    34081 ggatggcaag tgttggaaga agcttagaag atatttatca aaatatccag ctttcctttg
    34141 aatcgatcgt acgtgatcag ttcaaagcgg aaaagtctga aactttacag cgtaaaaaag
    34201 ccgtaatcgt gacatgcttt actggtgaag gagtagctgc caaattatat cagcgtatta
    34261 tgccagtgat taatcaaaat aaggtagaac tgatccagat gcagttttta gaacgtgaat
    34321 cattcaaaaa acacatcgat gccttattgg aagaatatga gatcaaagca attgcgggaa
    34381 cagttgatgt tgattatcaa aatatcccat ttttttcagc ctatgacgtt tttgatgatg
    34441 aaaaactaaa tattttaaag cgtatagctg atgatgagat cccgacagct aaaattgtcg
    34501 attcacttgc tggatcatta agcgatgtct tttctattga tgaactgatc cgttcattgc
    34561 aaaagacagt tcagcagatc cagaccgatt tgtcgattat cgtggaacct ggagtagata
    34621 ctggaatcgt tatccattta gccttcatgt tagacggatt gatcaaacaa gaaagccatc
    34681 ggcaatttga gaaactgtct attttctcaa aaatttatcg tttggaaatg gatatcgttc
    34741 gggcaaactt gatggcacta gaacgaaaat atcaggtaaa aattccagaa gatgatatcg
    34801 catttcttac acaaatgttt cttgaaaata aaataaaaaa atgaacacac taatcatact
    34861 gtttacacac taaacatctg tgtgcgcact ataaaaaaga gctaaaaagc atgaaaacgc
    34921 tatttcagct ctttttttta ttggcatgct tcttgcgtta tataaaagtg taaaaaaata
    34981 aaaggagtgt taaaaatggc aaaaaaaaca atcatgttag tttgttcagc agggatgagt
    35041 acgagtttat tggtcacaaa aatgcaaaaa gcagcagaag aaaaagggat ggaagccgat
    35101 atttttgcgg tctctgcctc agatgcagat aataatttag aatctaagaa tgtcgatgta
    35161 ttattattag gtcctcaagt acgtttcatg aaagctcaat ttgaacaaaa acttgcgcct
    35221 aaaggtattc cattagatgt catcaatatg caagactatg gcatgatgaa cggagaaaaa
    35281 gttcttgcac aagcagaaaa tctaatgaaa taaatcaagt atgcagttgg gggtataaca
    35341 acgtggaaga tcaacaaaat ctagaagcca tcatggggtt aatcatgttt ggcggtaatg
    35401 caaagagcga tgcaatggaa gcgattgcgg ctgctaaaat cggtgatttt gagttagcgg
    35461 ataggaaaat cgctgatgca gaagaatcac ttatacaagc gcatcatgca caaacagaca
    35521 tgttgacaca agaagcacaa ggaaatcata tgcaagtgac attattgact gttcacagtc
    35581 aagatcactt aatgacatct attgcgttta ctgatttagc aaaagaaatc atcgaacttt
    35641 atcgtcgcgt atacagcgaa taaatacaac ttcttttttg aacggatttt ttattttgtt
    35701 ataaggagaa ctctctttat ataaaaaact tataggagga agtaaaaaat gaacggacta
    35761 tcaagttgga tggagaaata catcctgccg gttgccgcaa aaatcggagc gcaaaaacat
    35821 ttagttgctt tgcgtgatgc ttttatcggc acactgcccg caacaatggc gggatcagtc
    35881 gctgtaatga tcaatgcaat tattcgtgat ttaccgcaac agtttaacag tacctatgct
    35941 gacagtttag cagcagacaa aggaatcttt gccgtattga atgtcatcat cggaatcaat
    36001 ggttttgtct ggaacggtac actagcaatc gccggattga tctttgcctt ttcttggggg
    36061 tataatcttg cccgagctta tggagtaaat gatctagctg gggggatcgt tggtttagct
    36121 accttgattc aaggtatttc ctttggtcca gcattatcag cagcattaga tgtgaatgtt
    36181 ccttcaaatg tcgcagatgc aatcaatagt tctgagattg gtgcaacgat tgcagatggc
    36241 acactgtcag ttggattatg gggttggcta aaactagatc atttgaatgg aaacgcttat
    36301 tttacagtta tgatcatggg agctatttca gtcattattt tttgtaaatt gatgcttgcc
    36361 aacattacga tcaaaatgcc tgattcagta ccaccagcag tatctaaagc atttgcagcc
    36421 attttaccag cgacgatcgc tttatacgtc attgctatta tcaattatat cgtaggtcgt
    36481 gtagctgatg ggcaactgat cattgatttg gtgcaaaaat atattgcaga accattccta
    36541 ggattatctc aagggttggg agcagtattg atcgtcacag tatttgttca agtattctgg
    36601 ttctttggta ttcatggacc aaatgtattg gcaccagtgc tagagggaat ctggggacaa
    36661 gcacaactaa tcaatattga tattttccaa aaaggttata acggaaaaac tggtacacca
    36721 gccgtgttag atgcaatcga agatggaaaa gcttatatgt gggtccgcgg atcgttcgat
    36781 gcttttgcat ggtttggcgg atcaggcgga acgatcgtct tactgatagc tatcttgatt
    36841 ttctctaaac gtgcagatta tctaacagta gctaaattat ccattgggcc tggtatcttc
    36901 aatatcaacg aaccagtgat gtttggtctg ccaatcgtat tgaatgcaat catgtttatc
    36961 ccattcttga ttgctccagt agtagcaaca gcaattggat ggacagctac ttatcttggc
    37021 ctagtcgctc cggtatcgca gcaagtcaca tgggtagtac cgccgttctt attgtcattc
    37081 ttagcaacag gagcggattg gcgggcaccg atcgtaacat tagtatgtat ggtcgtgact
    37141 ttcttgattt gggcaccatt tgtcatctct gcaaacaaaa tggaccctgc cgcaggcgaa
    37201 caacaataag atgaacgtga ggagcgggac gctttaagtt tcgttcctca taatgaattt
    37261 tcgaggagga actgaaatga ttacaattgc cagtgcaatg caacaagatg aaatcaaaga
    37321 attgttagaa ggatacaatg aagaaggaat gacttttaca tttaaagaaa aacaaggaat
    37381 caagctgttc tttgaaacaa gtgcagaaga tccggaagtt gcggctaaaa aagcaaagga
    37441 actaatcaaa gcacagcctt ggggaacagt cctttatttt caatcaacag ctgtttagaa
    37501 aatggtttaa aaagatagga tggttgagat gataaaaaac ggtttatttt tatgtagtat
    37561 tggttctttg atcgtgttgt atagtttgaa tccacattcg caaaggtatg atttgtatag
    37621 tatggtcaca ggtctttttc taacaggatt aggaatctat ttcttcatga aggggaaaaa
    37681 aagagaagaa gcaaaggaga aaaataacta atggaaatca cgaaaaaatt aaatattcca
    37741 gcatcgtttt tttacgataa agttatggac tctgtccttt tcgatgtacg taaagcgacc
    37801 ggcaaatcag taactagaaa acagctgaaa aatttggagt acgtcaaaca attctcgcaa
    37861 aataaccggg ctcgcataaa aatcgaagaa gtcattgaaa accagtctta taagtttcgt
    37921 acatcgacaa cgaagaatga attcgtcgtt cattatcaaa tcaaaccatt ggacgattcg
    37981 acctgtgagg ttcattacac agaaaaaatg gagtcttatg gcatgcttca aaagttgaat
    38041 gatatgcttt taggaacaat cttgatgtat tttaaacgga gacgtttcaa aaaaatgcta
    38101 gatatgatcg aagcaacgta ttaaatcaat gaaacatggt actatttagt tattgaaaaa
    38161 tgaagtaagc tagaaggaat ttttcatagg aagatagatc tttcctgcga atcctcacta
    38221 gcttttttgt aggaaggggc agcaaaatga caaacaaagt aagagagcta ggtgtatctt
    38281 cgattgagga aatgtttgat ttagccactt atgcatttaa tggggcagat acacctgaaa
    38341 ggcgagaacg ttttcgtaca cttgcggaga attcttggaa ttacggattt tttgatgagg
    38401 aggatcggct aacaagtcaa gtcatggcga ccccttttcc agtgaatttt tacggacaag
    38461 agtacctaat ggccggcatt ggctatgttg cttcttatcc agaagcaagg ggacaaggcg
    38521 ggatcaatca gatcatggaa aagatccttg aagattgccg tgatcgtaaa gtaagcttat
    38581 cttatctggc gccattctcc tatcccttct atcggcgcta tggctatgaa caatcatttg
    38641 ataaaatcag ttatcaacta aacagtcgtg atgtgccgtt tatcaaaaaa aaatctggaa
    38701 aaataaagcg tatggatttt gaagatgcaa aagctgcaat cagaagaatt tatgaacaga
    38761 tgccggaaaa ccaacgagct ggtctgaagc gcgaagactg gtggtatacg tataaattca
    38821 agcaaaaagg caattaccag tttgctttgc attttgatga taatgaggaa gcaacgggat
    38881 atatcgttta tcagcttgca acgtcttcct ttattattgc ggagtgggga tttttggatc
    38941 atgatgcttt ttgcagcctt gttcgcttcg taagttctca caatggtgct tttgaaacat
    39001 ttgaatattc atgcggctac ggcggtaatg atcaaaatta tctattagaa aatccatttg
    39061 cttctgtaat gatcacacct tatatgatgg cgaggatcgt agatatttct ttgtttttgg
    39121 aaaattatcc atttaaaaag agacaagctg catttgccct acaaatagaa gaagatcatt
    39181 atgcgaaatg gaatgaagga atcgtggaag taacaatcga aaatggaaaa aatcatctct
    39241 caaaagtaga acaaacaaaa ctcccaatcg ttcgaggaag tatccaacat atcacgcagc
    39301 tattattagg gtatcgtaaa gtggaagagt tggtattcca agaaagaata caggtgacaa
    39361 aagaatatga agaaacgtta agctcaatct tgccacagca acagccaatt cttaatgact
    39421 atttttgata aaaaacgggt gatgacaata tttttgtcac cacccttttg tttttcattt
    39481 ggcatttgaa ggaaaatgtt aaagtaaatc ctcttttttc gtaaatttga attttttgtt
    39541 ttcttttgtt ttagggtttt cttttttgtg gtcttggatt gcttctttcg taatcttttc
    39601 ccgatttttt ttagatgaat ctaagcgttc tgcgtatact ttttcttttg gcttatcatt
    39661 cattggaaag gacctcccta cattatttca ttctacctct agtttatcat ctgttaagga
    39721 ctagtggaaa cgttttgcct aacttataag ctgatttttt tagcagataa gcttgcaagt
    39781 aaaaaacaaa caagctacaa ttgatttatt gaaattgttc ctgtagctca gtaggataga
    39841 gcaatcgcct cctaagcgat aggtcgctgg ttcaattcca gtcaggaacg tataaataac
    39901 ccctgtatcc tttgcggtac aggggtttgt tttgtatttg tgccaaaaat gtgccaaact
    39961 agttatcatt tttatataaa ctatcgaaca cagtattgat tttttcttta tcctgatctt
    40021 caagctctcg aacaatatga agataagtag acattgtagt ttctaaactg ctatgtccta
    40081 aacgtttcga tacgctaagt atatttactc cttgataaag taaaatagat gcatgtgtat
    40141 gccttagtcc atgcaaagta aattgctttt caatccctaa ttcttttaat tttttgcgca
    40201 aatatttgct gacggcgttt gatgaaacta atccattttt taagttgaaa aaaacaaaat
    40261 tatgagggtt tttgatttca aaattctcga ataattcttt ttgattgatt ttaaactttt
    40321 ttaagagatc aatcgtatgt ttatcaatgg aaattttcct atttgaagtt tcgttttttg
    40381 tttttcccca ttcatttaat ttataattcc atgttttatt aatagtgatg atttgatctt
    40441 cgaaatcgat atcttcccat gttaatccta gaagttcagc aaatcgcatt ccagtggcac
    40501 cagcaactaa cacgagcata ggagaagagt actgcgcaga aaggttttct tctacgagtt
    40561 tcattaaaga tttaaattga tcataatcta aatatttatc ttcttttggc ttcaatgaat
    40621 cttttccttt gattactgct tttctagttg gatcaaacgg aattaatcct tcttctacgg
    40681 catctttcaa cgacgctcta atatggttat taaacttaat aacggtagat tttacatggt
    40741 ctttagcata cttatttaaa aagcgttgat atcccactct atctaaatca gaaatcaata
    40801 ccgctggcat atatttcttt atgttcatta acgtatcttc gtattttcta taagtgatag
    40861 gagatactgt tccttcttta taaagttgca tccaatcctc aaagtaatct gataataata
    40921 gatttttgcg atccccttta aggcctttgt tcaagttata ttctaattcg ttggcggcat
    40981 cttttgcttc tccttttgtc ttaaatcctg attttcttat cttcgtgtat tttccgtcat
    41041 cttttttgta tgaaatttcg tattgccaac tatttccacg tttgactaat cttgccataa
    41101 ttgaatcagc tctctttctc tgatacaata ggaactataa agaagcccat tgtttgggtt
    41161 ttattatttt ttgcacatcc tttttgcttg ccggcgcaag gatgtgctat ttgtcgtttt
    41221 aatggtataa ttggatgtgt attagatcat ccaatcagag gtgtttatta tgattaaagt
    41281 gatttttaaa agaataactg aacttccaga gtttagatta tttgttgatt attggtatgt
    41341 ctggtttttt attgttgcat tgctgatagg tctagatgtc atattagaaa gaaaacttaa
    41401 gtaagagttg agttaataaa ttgaattcct aaatgagata ttttgtagcc catattttgt
    41461 ttgccgacat cacctaatac tatggagcta atcgtttttt ctaacagacc aaaatcagat
    41521 aatctattta agctaccatc tattagctct tgtgaatagc cagacatgct gatatttcca
    41581 gtgaaatttt cgtttccacg actcattaag catgctaaca attgaatctc aatcgatgtt
    41641 aattgattca gtacaagaag ataatactcc tcgttatccc atgaagcgtt gttaattcga
    41701 agtaaacaat tcttataaag atttttatac atatctattt tattttgtgc ctttgatttt
    41761 tctacctcgt catttatccc ttcgaatatt ccaatgagtt ggtccttgtt ctctaagttg
    41821 aaaacatctt ctggaatttg atcgcgaatg ttttctaatg attcgtttaa atttttgtag
    41881 aatttctcga tgcgtttgaa acgcttctcg tttttagacc caaaataaag tgactgcaca
    41941 acgccaccaa tataaggaat tgctccaata ccgccttcaa ctattaattc aagatattct
    42001 tttttggtta gtttttcatt gctattatcc atagttacct cctattttgt catttccttt
    42061 acaagcattg tgactcctga tggcactctt ttgtataggt tttattattg tgcacaatct
    42121 aactcttagt ggggcgtggt tcatttattt tttacttact ttactattaa tcatatccca
    42181 ttgagcatag gatttttcta tgttcgatcc tcttgaacta atttctcctg taaaagttac
    42241 tttatcgccc tttgtaaagg tatcagcatt gtttaagtcc ttcgcaaaaa ttacatatga
    42301 aactttaggt gttgcagaaa tatcattcca acttttatca tcgtactctt tgtctgctat
    42361 aatggctatt ctagtagtca ttgattcgat aacagttcct tcaaaagtat atgttttacc
    42421 actaatatat ttagtaaaag ctgtattttg ctcagtagct ggagtaatag aataataact
    42481 atctaagaaa gcaccccagt catgattatt tgtctctaaa aattcttcaa attgagttag
    42541 gcttttattg ctgctgttag atgtagtagt ttgagtatca gatgatgagc tttcctgttt
    42601 gttattgtca tttgcctcgt ttgatgagga aagcgagaaa cctacggtag taaaaataat
    42661 cccgataata aggagaatca taggtgtttt cttatttgtt tttttgataa atcttacaat
    42721 taacatgatt aaacctataa taaataaaac aataccagta aaaccaaata taccgtccat
    42781 taatagatcc tctttctctt ttgatattga atattataaa ccaagcaatt cttttttctt
    42841 aaaatcgaat tcttcttgat taataatccc ttcatctaac aactctttat actttttaat
    42901 ttcatctgca cttgaaattt gtgtttcttt ggaattatta tctaatatag attgttgctt
    42961 ctctatatat ttttttaaat tttccatttc attccaatat ttttttgtaa acataacggt
    43021 gttttcatct ttcgtggccg ctaatatacc tccgcgactt tcgtttccgc ctagcaaggt
    43081 aaattgtata taaccattac tcatacctgg tttttttaat tgtacggctg atatattttt
    43141 aaaggggata gttttttctc ctttaatacc ttgattaact aaatttataa atccttttct
    43201 aataattgaa atagaatcat cagttacttt aatcaaggtt tttcctggag attttattaa
    43261 aatctctttt tccatttttt cttcctcatt tctgtgatat gatttttttt gtagaagctc
    43321 atatatgagg aaagagcccg tgttgcagca cgggcttttt taatgttttg ctgaaatcgg
    43381 tttttttaat aattcttgat gtattcatac atattgcctt gcgtaagaat attttttctt
    43441 aaaatagcat tggcagacag cataacaagt gtatctgagc ttattctgaa tagaataata
    43501 ctccataaat ttttcgagat taaactgaga tttatcagtc agttcgttct caatatagat
    43561 atttaataaa attagaatag ctattttatc tgcttctgtt tcaaattttg agtgaaaagt
    43621 tgtagaagta tcgtacaaaa ctgaaaattc aaaatgagag gcgctgaaat gtgcaagttc
    43681 atgagataaa tgaaaggctt ctgcagtttc accgtataga ttttcattca aaaaaatgat
    43741 tctgggtttt ggatagtaga aaccaggctc tttcatctcc atataaacta ctttcaaatt
    43801 gtattcgctc aacatttctt tcaatttcaa atacatacaa accatcactc caactattca
    43861 ttttcctcta aagctttagc aattgcaatc gctttacgca ttgtctcctt agatatttct
    43921 tttccatcaa aagaaaaaac agtatcgtct tctgataaat ccacatgttt aggggtttct
    43981 ctttcttctc tacctagaag gtagtctaca gagacatcga aatagtcagc aatccgactt
    44041 aattcatctg aattaggagt attatttttc cactttgcta gataaccgtt agaatatcca
    44101 aaattaattt ctaactgccg tatagatatc tttttcctct tcgccaagtc ttttattatt
    44161 tcataggtat tcatcgataa aacaaccttt ctaaatgttt acaaaaaaag tttagaaaaa
    44221 tacgcagaaa taatttgact aattctgagt aatgagctat actatatctt gtaaacagat
    44281 ttaacaacta aaaagacaac aaaaaacact attgatatgt aaatgcagac cgccaagaaa
    44341 gctttaaaat caatgttttt atgtcttatt taattatgct cttagtatag agtattactc
    44401 agatactgtc aattgaattt agaaaaaagt tgttaaattt gtttacgaat ataaaagaaa
    44461 ggaatgagaa atatgaacac accgcaaatt tttaatttcg aacaaaacga agttcggaca
    44521 attttagtaa atgatgaacc atattttgta ggcaaagacg ttgcgagtgt tttgggttat
    44581 tcaaacacta aagatgcttt gtcgcggcat gtagatttgg aagataagat ggggtcgcga
    44641 atcacgacct caggtcaatc aagagagatg acaatcatca acgaatctgg tttgtacagt
    44701 ttaatcttaa aatcaaaact tccctctgcc aaaaaattta aacgttgggt aacaagtgaa
    44761 gtgttgccag caattagaaa acatggaggt tatctaactc cagaaaaagt agaagaagct
    44821 ttgcttaatc cagatacaat cattcaatta gcaactcaac taaaagaaga aagaactgga
    44881 agattaatcg cagaacaaaa gattgcagag tacgaaccta aaatctccta tttagatagc
    44941 atattatctt ctacagattc agtaacaatt agtcagattg cagcagatta tgggatgtct
    45001 ccacaacaga tgaataaatt acttcataaa ctaggtattc agaaaaaagt cggtaaccaa
    45061 tggttattgt gcaaaaaaca catgaaccaa ggatacacaa aatctcatac aactgagatc
    45121 ccgaaagccg atggtggcac taaaattgta atgaatacca aatggacaca gaaagggcgt
    45181 ctatttatct acgaattact aaaaaaagaa ggatattacc ctcaaatgga tttagaggaa
    45241 attggttaga aaggagtttt agtatgactg acattgcaga aatcactcaa cgagatagag
    45301 aaaaaatcaa agaatatgtc gaaagttcaa agttcttaac ttacaccatg cttgctgaaa
    45361 gatttggaat tagcaaaagc tacttatctt taattttaaa cggtaaaaag acttctgcag
    45421 aagcaaacag aattatagat tcgattatca ctatgtacga attgtaaagg gaggaacagc
    45481 taatgcaata tctagaagca aaaatcccaa ttccagaagg ctatgtaatt atctcccaag
    45541 tggattatga ggagttaaaa aaagctgatg atactggtag atggatgacg ttgccagaag
    45601 tgctagaacg gattaacaga aaatatgatt ggtttacttc tagagtttta aagaacccaa
    45661 gatatagaaa tattatcgat atcgagaaaa acaaaaatgg atttgtctat tatccagttg
    45721 aaggaagaga cacatatcta tttttaagaa gtaaaacact tgaattttta gaaacaaatt
    45781 tttcggaaat cttaaggagg caagcggatg ggaaaattta atagagcgct agtgttcagc
    45841 gcaccgctaa tcatctacgc tttaggactt tggggaagca ggcaagcgtt gataggaacg
    45901 attgtttaca tggtttggat ttttatgggg cttgatgaag ctgaagctga gtacagagcg
    45961 aaaaagccaa ccgagggggc tgactaaaaa tgaatagaaa agagaaacta gaatgtatat
    46021 tactattact cagtttaatt ctttcactaa tttctctatt gggaagtttg tatttttgat
    46081 atcaacaatt ttgtggattt gggttctatt tagagtaaat atcatttttg tttggtgatt
    46141 aaggagttta ttaaaattaa ttttccctac ttcaaatgca aggtaacaag atatagcact
    46201 tttaggagga atatttagcg gtacagcttc agagtatatt ctattatcat cgactgatcc
    46261 tcctttagca attaatttct taaatggagt agctgaccat ctgcggttta agttagcgga
    46321 tgaaagttct aaattaacta acatagctgg ttctgttgaa tagttagaaa tgattacttt
    46381 tgtataaatc atatctgcag cgaagtaaga agcattaagt tcaacttcta gttgagggcg
    46441 cattcctttt atttttatcc ctgttaaaaa cgtactcaat ataaaactta ttattgacat
    46501 ccattgaaaa attgttaagt gaaaaaaatt caaaataaac accaccagtt tttaactaaa
    46561 ttataccaaa aaggagagaa gaaataatgc aagaattagt aattttgaaa aataaagaag
    46621 ctgtgactac gagcttacaa gtggcagaaa gcttcgagaa aaaacatcaa catgttttaa
    46681 gagatattga tgcactaaaa aaagatgtgt ccaattttgg acagatgttt gtagaaggta
    46741 atgaaccaga ttcatatggc agaaatcgac gagttttctt cattagtaga gatggatttt
    46801 tcttgctggc tatggggttt acaggaaaga aagctatcta cttcaaacaa aaatacattg
    46861 aagcattcaa cgaaatggaa gatgttattc gcaagaatac tgttcctcaa acaattgaag
    46921 atatgatgat ctatcaatta gaagaaatga aagatgttaa aaaagatgtt tccatgctta
    46981 aagatactat gcgaattagc ggacaacaag agtttgaaat taagcaaaaa ggaaatatga
    47041 aagttatggg agttctaggg ggaaaagaaa gccgagctta tgaagaaatc agcaaaaaag
    47101 tattctcaaa attttggtct gaatttaaac gtaccttttc aatcccaaga tatggcgagt
    47161 tacctcgtaa gagattcgat gatgctgttt catttattga aatgtggttg ccagaaactg
    47221 cgatccgtat ggaaattgat caactgaaca gacaacagag actttttggt gatgacaatg
    47281 aatagagctg aagcgctaag aatagggacg gcaattgcta attgctggtg gaaatactat
    47341 aaaccaatca tcctaagcca acaacatatt gacaagcaaa aagcatggca acaaataaaa
    47401 aagtgactcc gccggcaagc aaagagtcac aaagaaaaca catcataagg agattttagc
    47461 atatggaaaa agaactttcc actctagatc aatatttgat tgatcctgat tggggcaagc
    47521 cgaaaattga ggaaacaagt ggtcgaaaaa tcagacgaaa tcttttgacg aatgaagaac
    47581 tagcttggga tcaagatgat ttaggcaacc atgtaactat ttgggatcat gtttatctta
    47641 tccatctatc gaagcattcg aataaacctg aatatattta cgtcatcgaa gatggcttga
    47701 ttgatgcgct agaagagtac gacagagata acttgattga tatctcttat tacggaccag
    47761 gtaagaaata cattgctgaa atggaggcag aatttgatga gtgaaatcaa agggacaacg
    47821 aactttgaaa aactttttag tcgtaagtta aataaaattc tcaagaaaaa aggaaatttt
    47881 gattatttat cttgggctca cgcgtgggag attatgaaaa agaatgatcc acaggcaacg
    47941 gtaactatta atgagtacaa acactacagg gttgtttctg gaactcatca agactttctt
    48001 gttgaggaat ataaaccttt tcttatggat gaaactggga cttatgtatc tgtctcagta
    48061 acggttaaag gacacacgga aaccgaatta tttcctgttt tagattatcg aaaccaacca
    48121 gttgttaaac caaatgcaat gcaaatcaat aactcattga agcgatgctt tgtgaaagca
    48181 ttggctctac acggactggg attatatgta tttcaagggg aagatattcc aacaccacct
    48241 agaatcgata caaagaaatt aaacatgcta gagacgattc tagaagcttt caatgagcag
    48301 atgggtaaag atatgaccaa aaccttaatc gaatatgtta atgagcagac agataaatta
    48361 gggctcttag ctgataacgt tgaaactatt gaacagttaa gctatgagca atgtgccttg
    48421 atggagcgag caatagcagc taagagaaaa gaattagata agaagtgata tgagtgttta
    48481 aaccattaat cgattcatat tcagcggttc tgaaaaagtt caaaggaaaa gacataagcg
    48541 caaccatcaa tgaggaagtg aacattgatc gactaaagac gatgtatgac ggctacgatg
    48601 gtgatcgagt cattgaaatt cgttttattg atcctagacg tttcaccgta cagcaacgaa
    48661 acttcatcta tgcgctgata ggcgatattt ttatcgatac aggcatgcca acggacttct
    48721 ggaaggaatt cttctacttt cgttttgaag gtgtcacagg gcgcaaaata agcctgaaag
    48781 acgaatcgaa tacaactgtg agtgatgcca atgtcttagc aaatatcatc ttagatttca
    48841 tctttgaaca tcatattcct ttcaaagaag gctatgagat tttacctgcg aatcaagaat
    48901 attacttcta caaatgcatc acaaaaagag tctgctgcat ctgtggcaaa acaggagctg
    48961 acatcgatca ctttgacaaa gcgctaggaa gacgaaagcg caaagaagtt gatcatgcag
    49021 agtacacatt tgcagcactc tgcagaatcc atcacacaga gaagcacaaa ataggtgtga
    49081 ttaatttcaa aaataaatat caaatcaaag ggatcaaatt aaaccagaaa acaatcaaaa
    49141 agttaaatat tggagggtaa aaatgacaga acatcgaagt tattatgcga ttataccagc
    49201 caacgtaagg tacgacaaaa gacttaaacc aaatactaag ttgttatacg gagagataac
    49261 ggccttgtgt aatgaaagag gcttttgttg ggcaggcaat gagtactttg cagatttata
    49321 tggtgtgaat aaagagacca tatcgcgatg ggtaagtgat ttgattaagt ttggatactt
    49381 gaatcgggaa atcatttaca aagagggtac caatcaaata atcaataggt acctacgaat
    49441 taatcaatac cctattgacg aaaaacgcaa tacccctatt gacgaaaaag tcaaagataa
    49501 taatacatct attaataata catttaataa tacaaaagaa tatataagag agttaccgcc
    49561 ttcgaaaaaa tcgaaggcta agcccgtccg tcataaatac ggagagtata aaaatgttct
    49621 tttgtcagat gagcagatgg agaaactcaa aacagaattc cctaatgatt accaagagcg
    49681 aatcgaacga ctgtcagagt attgtgaatc atctggtaag acttataaaa actatttggc
    49741 aactattcga agttgggcaa ggaaagaaaa aagtgaacct aagaacgcaa gcagtggata
    49801 caagcgcaca ggaagacgag agaagcttcc tgaatgggca atcgaccaag aagcctatct
    49861 caagaaaaaa gcgctagaac gagctaatag acaatcaaaa gcaccattct aagaggtgga
    49921 aaaatgaaga tcgattatct agaactaatt aatgaaatag cgaattataa aaagggcgag
    49981 gaattagacg tcctgagaga cgtatatgat caactcgaag aagctggaat tgaaggaatt
    50041 aagaatgatc gttcgagttg gagtaagctc agatactatt tcgcacttta tatcgataca
    50101 acacaattaa gaaatttagc atataccaaa ttactatttg ttgattgtgt taaaggcttg
    50161 caaaaacatc ttaatgaact tgagcaggtg taatcagatg gacctaaaga catttacagc
    50221 acagattgaa ctaatgcatc aagaagcttt aagacaaagt gtatcgtacg aagacaagtg
    50281 gctcaacacg ttccatggcg gacgtgagag cgcacttgat caagtactca aattactgaa
    50341 aggagaatgt cgggatggat aagaaagcgg caatgcagcg aattatcgaa ttgacttatt
    50401 cagaagattg gcaaaatgac aaagaagctg cttcagaagt gatgaggctt ggaagagcga
    50461 tgtgggcaga caagagcaac aagccaagac cacgaaaaat cgcaatttgg cacggtgaca
    50521 aacttctagt gatagggaca gctgaacagt tagcaagtct cacaggcttg cacgagaaaa
    50581 tcgtgaggaa aagagcaagg tgtggctaca cagacgctaa gaagagaacg tttagatacg
    50641 tggaggaatc atcatgacaa cagaagaagt gattcaaatg cgtattcgaa gccttcagcg
    50701 tgagattgac gatctggaac gaacaaaggc agtgatggtc aatgaaacgg cgagaaaggc
    50761 aatcgatttg cacatagaga atttaagaag ggaaatccat cgattggagg aatgagcgtg
    50821 gataagaaag cagcaatgaa aagaattgct gaattaacca agtcagaatc ttggcaagaa
    50881 gacaaagaaa tagttgcaga agtccaaaag ctcggtaaat caatgtggac tgaaaagccc
    50941 aaacggagaa cgccgagaaa aattgcaatc tggcatgatg accgaatttt agtaacaggt
    51001 actgctgaac agttatctga aattactgga ttaagcaaaa acattatctg ggatagagct
    51061 aggagcttat ggattgattc aaaaggacga cagtttaggt atgtggagga gagataatgg
    51121 atctcattac acaatacagt gacatcatcc tcaagaaaat catgatgaag attcagaaag
    51181 a
//
LOCUS       DACKAC010000013        48393 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.013, whole genome shotgun sequence.
ACCESSION   DACKAC010000013 DACKAC010000000
VERSION     DACKAC010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.013"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..432
                     /locus_tag="I0B46_000795"
     CDS             <1..432
                     /locus_tag="I0B46_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083116.1"
                     /translation="IDENGQFFVWMRHEHCWCNPFVLKKENEIRTKIANGEWDYLFRW
                     EDESFIANDMDHAFVSFLDNTWGTWYDCYLEKLGFQNIDIDAIECLKEVDQKYEEEPY
                     YLLQQKLANNSVFQVEIWENEDGTYEGLAQEIPFSDRKSSI"
     gene            607..942
                     /locus_tag="I0B46_000796"
     CDS             607..942
                     /locus_tag="I0B46_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083117.1"
                     /translation="MKQYLNEWKAIEGSLVTQRIKELPDCLEKDHLFQIREMLKKEQF
                     DPDQFLVVEYPTIGVYCCNHINDGKYFIIQEYGGQLTPFYTTWEMSEEGINNFPCESI
                     EDSISHTEC"
     gene            complement(1199..2782)
                     /locus_tag="I0B46_000797"
     CDS             complement(1199..2782)
                     /locus_tag="I0B46_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083118.1"
                     /translation="MFILDDEIKKVKNPRIIPLLEEVRSSFYQGNYRSAIAVNYSALI
                     LDLIDKLSDLANIYEDNTSKKILEDIARLRKSDPNSPKWEMELLEKAYSSTELIDSYE
                     YEDLKYIKDQRNYSAHPVVTYNGNTWELRDITKETCQDVIRKSYEIIFLKNPILGKKV
                     TIEIIQYASRIHAMSVTPEEFHSALKNSYLNKLSEKAKENLCKTAFKFVFQLSYGNEE
                     ADRNRESTFRLLNALIFDNKEFYLNILKKEIAKYRFTAESREEINKSLGENQQITYTK
                     GFFLLTFIATHPELQHDFSDETIQNLQISINDFKPKQQVLTEIEYYYRLRSLAVLGNN
                     NEELQNHLNSLVDPILQQKMRVSSLPSSTLETIYNQYLYFGEKEMFIDFMIEHLTDAS
                     SFRLADKDMEVLPWIYNKLNQEQFNKLLYNLNGNNQFYKNSNFPPFISNLLDFYNQKF
                     QVNLRKHYSGLLYPNAVTMDQGFQLKDKELKKLYELAESKSDMYLDIIDNIINKSEDY
                     LRKEINSEKELLNYVHKIK"
     gene            2904..3488
                     /locus_tag="I0B46_000798"
     CDS             2904..3488
                     /locus_tag="I0B46_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083119.1"
                     /translation="MDLKQSFYIWLYPDNPMSIMADIFVVSILLYLFYLIFFHNLIGY
                     MILDWKGMFDIERKPFANNQTYTVFESGDWEENEVDVIHEIGLALSGIQDADVLVSMT
                     TDTGYNAYEVFYLMYFIWRDGQEIINGRAEKTINSNTEISFCMGEGEMIVYKDGSANI
                     KTAFGDIETSIKELREYVQNPYNFQYLAGRINLV"
     gene            3499..3744
                     /locus_tag="I0B46_000799"
     CDS             3499..3744
                     /locus_tag="I0B46_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083120.1"
                     /translation="MKKIINQPTNHLIIEGVIFVTMYFTVGNGISNTIIQKNPQYLLF
                     SLYAWIVQMVWVSILMLLFYWIVRVILLLLNKRKKDR"
     gene            3750..4472
                     /locus_tag="I0B46_000800"
     CDS             3750..4472
                     /locus_tag="I0B46_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083121.1"
                     /translation="MKSTKLKVVLLSTAALITLTACGTTNSANNNKDTKSSSTMVTKN
                     STTSASTTMSSMTENNAEKYQKYEDINKGQKPDEVYNNFINTVKNDENYGNDPLQNKN
                     LTIVLYKPDCPDCQKAESKIMQEIKEGFSYAPTLMHDITINKPAYEYYAVAVDITDGV
                     PEWVQNIPGLADENNQYHTPTVAVVTPKKVNGTLYWDVMGMTTNTTDEMIESTTKYYM
                     DLGAYKLYQQSEPVMDDQSKVK"
     gene            4603..5346
                     /locus_tag="I0B46_000801"
     CDS             4603..5346
                     /locus_tag="I0B46_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083122.1"
                     /translation="MKEISANEAHIPQKNQVGIYKVLHGEGIYKIQLLTGYNNGANGK
                     WLKATSIRELRSEVEKIRLGLTSEEIAKLKGNNSFVIEKEKEKHLLYRELKIEEYYEI
                     DLPEKLRIIDQRSEEKWKTEIYPSLNDYKTSGKPMYNYTGFLTYTQYKYAFRKLKGIK
                     QAEIALYEMKKVDRLKLIQDLVQNTWNQAIVELEKENGKILTNKYLIVNEIEELIGLF
                     SCEKGEVFLKSSPTYSILDSPYQFIQARR"
     gene            5352..5717
                     /locus_tag="I0B46_000802"
     CDS             5352..5717
                     /locus_tag="I0B46_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083123.1"
                     /translation="MSSLKDDYIQAKQEYEKIQMLKSEKVELFLTQKLAEDHIANLDA
                     KTLEEYDRQFNQIHHNIILLVEQAKRRFEKAEYDLLDQGIVCLDKGLRRYYGAELKKQ
                     NVDRNALKHEILELIMQRI"
     gene            5733..6263
                     /locus_tag="I0B46_000803"
     CDS             5733..6263
                     /locus_tag="I0B46_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083124.1"
                     /translation="MSQNISAVKQLIEEDLETTVQRLAERIHFANYQTISNWSSRNRN
                     PEEIPMKFFYRIGKEYGVDPIKIFEKTMIYYYHGTPNEVLEHLLEEAKEKSSILELSQ
                     NDKVIQVKCVDPTGKIIVGFDIERKSKIDFITQLKKINNLDILTLRPDHEQLFMSFKK
                     QLKEIQKRYNKVFPNA"
     gene            complement(6859..7338)
                     /locus_tag="I0B46_000804"
     CDS             complement(6859..7338)
                     /locus_tag="I0B46_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083125.1"
                     /translation="MTDKTSDLPKLAPKTSRVKSKKDGKTKNAVKKKSVKETSSLLLD
                     IPMSSFDDNTDETSVKIATHVGMNNQNDSFYNLLNSLRELEDKKNQVIASKAPDLTTD
                     LDAEIIQIKQDERSVKSLLKTYFDGFDKELAPYSQAILTKPIQAPHISISLQKKSDD"
     gene            7458..8795
                     /locus_tag="I0B46_000805"
     CDS             7458..8795
                     /locus_tag="I0B46_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAR2083126.1"
                     /translation="MLMVQFTGIAMSVITINPQVIHADGIATYDAQFNCTTEGSGSGT
                     DSDGNDTGSGAVTGDVAKNIEDIYKYMNGEYGLSGASIAGILGNWKQESGIDPRAVEG
                     YYGDGQREVDFIKQFTDGLMGIGYGQWTAGRHTALVDWSKKNNGGSWWTTEAQLDFMF
                     KGDGGFVGILKDYALNASDDVVENAVTFHKNWEISADSESAVIAVRGENAKEIWKYMK
                     SKGMDGAKDESKINKISGGSSTGDGSNNSSSSASGTVEDACATTDDANSGSGSIGKEG
                     KINGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFNGIYQGQCTELTWAYMS
                     QLYDGNQPTTGNGGYVWESYKSAGAKITNKPIVGYGFSASDGWLWAVGDAGHTGVVIA
                     VFEDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFSGVGKAKFEIEK"
     gene            8818..9345
                     /locus_tag="I0B46_000806"
     CDS             8818..9345
                     /locus_tag="I0B46_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083127.1"
                     /translation="MNSKVKDTIIISLLSGMLLALLVGILFFNKQNQDIEAEKHSVHI
                     EDKLTFFKEVKPNEVDGLRKDLENDIQEMLNGSVQNKFADQSGEEFLRKLFIVTGKPP
                     ITDKSSEKEKIEYYKPFTFKLTNFGIKTSSSGLYEALTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            9332..9895
                     /locus_tag="I0B46_000807"
     CDS             9332..9895
                     /locus_tag="I0B46_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083128.1"
                     /translation="MQNHEITTAKKAIGILSIIVILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRKFFNLFYNWNSWEKYTKNMKEIQ
                     KEFPQIVKSSPVDISGTMVGTGNSPISSYTADYYTTNQKGKMVELITQTRNTPTTATT
                     TIWRGVTSIGENQLFYLEDLDSYVRAE"
     gene            10318..11055
                     /locus_tag="I0B46_000808"
     CDS             10318..11055
                     /locus_tag="I0B46_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083129.1"
                     /translation="MADRKLEKLLEETWNPKEFSEFFMENFETDLAVIVKDALREQGY
                     PETANYININFTLYTENKGTWDFWATLANKELSDKSDTGIRNFFESNRDDYMYANHQD
                     KLNFRVEFDETPEEFIERQPPKENVAKVLEDRWNSDEIVSTISELGGQYEPLVEAVRE
                     ELRLNKFPDVQNIDVSQIEINVKITNKLDYGSWADIALEKYIYSTLKEFIENRMDIMY
                     LQHPQYLNFGVEIATPLEEWKMEQGLD"
     gene            complement(11072..11488)
                     /locus_tag="I0B46_000809"
     CDS             complement(11072..11488)
                     /locus_tag="I0B46_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083130.1"
                     /translation="MNKKRKLSVLITLVSILTILSGILSLKIFNQINTSSNAKDGIVV
                     ENTSNSKETVTTTKKDKSAEITTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDK
                     FNGTFSLQTAEGSPDNLKVGQTIILGKNDELYSFVK"
     gene            11880..13127
                     /locus_tag="I0B46_000810"
     CDS             11880..13127
                     /locus_tag="I0B46_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HAR2083131.1"
                     /translation="MNMKKNSLGMVLFASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNISK
                     DTLTVKEADGEVVSQTVQKDKPDPVENTEQENKKSETAKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTETDTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSKPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGVANGGNVVDISGSSVQ
                     GLKDEIVNGNPVVAWVTYAFEPAQHKSMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINAGSFETAYNVSQLAVSVR"
     gene            13397..15787
                     /locus_tag="I0B46_000811"
     CDS             13397..15787
                     /locus_tag="I0B46_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HAR2083132.1"
                     /translation="MKHEKTIILKSKIIYCFATLVMVLSTLIMPFSAIRAEADVTKPT
                     SAKFTETGVKTDGYIRVNMPNHPNEWMISSQFKDSHGNIGYCMDSELPSPTGSGAGSL
                     KYKGAGSDEFYRMFKGGFPSKTAKELGAGNDTEAWYATQLVSWVLAGNFKVSQIVWSH
                     PNHTAAETARVKKAFEKIYDYAKNGKDTPNTEFSITASKTADEGKYHTFTYKTASNKT
                     GNAKLTFTSAKPAGMKIYDADGKEITNNTVKLNSSFTIKVPVTTPSGTLSFKGTANVS
                     TTNPFTFDGRGVYQDAVVMITTSETKDSKSLSAKWTRAVGQVTVKKVDQDGNALKGAE
                     FTLTDNNNAKSVKTTGDDGLATFDLQQGLTYSLEETKVPEGYQGSFKKDGITVNTDGQ
                     KFSYTATNTIKKGKVKVKKVDQDGKPLANAEFTLTDDSSQTQAEKTDENGLASFDIVA
                     NKTYSLEETGVPTGYTGAFKQEGITVKDDGQTFEYTATNTLKKGVVKVHKVDQDGKPL
                     ANAEFTLTDEKGATQVEKTDENGLASFNIVANKTYSLEETGVPVGYTGAFKQEGISVK
                     DDGQTFEYTATNNLKKGVVKVHKVDQDGKPLADAEFTLTDEKGVKEVKKTDATGLATF
                     NIIANKTYNLEETKVPTGYTGAFKQEGITVKDDGQVFEYTAKNTLKKGVIKVHKVDDQ
                     GKALANAEFTLTDDKGVKVVQKTDRNGLATFNIVANKTYKIEETQNPNGYTGKWSKEG
                     ITVADDGQTFEYTVINHLVPETVRTGGIGTSAFLLVFLIAGLSMVLLYYREQHKAK"
     gene            15836..17671
                     /locus_tag="I0B46_000812"
     CDS             15836..17671
                     /locus_tag="I0B46_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAR2083133.1"
                     /translation="MKSKKIFSNLMLFGTLLGVGLGASSISSSLGLGGQVAYAQQNYN
                     YQGTGTIHIHKVTNTNGDGNPVSDIDNKGKEIALPNGVKPLEGVEFSYVKLTKDEAKS
                     LGWTDSDTTKGQHIDDAKARLLLNKKYSEVSRMKTDSQGNIDIPIQVNTDDLTDNLYL
                     IVETGTVKGVVEEAYPMIVNVPELNGSDDQYFNYDVHVYPKNYFQLNSAELDKKDEDG
                     KALAGTVFELRKDDGSDQVVGTYKTDSTGKLTVDGLELGDYYFLETDLGTDANRTTYL
                     LDGKKFKFSVKRGETAKVVLDVTNYKRPVITKVIDKETVDTGEDVQYTLKPSIPGNIG
                     NYEKYVVKDVLGAELTYDSSEVIEGMTLDTDYSIERDGQTTNYVFTESGMDKLQKQFD
                     AGDRFFTILMNAHTNSKAKMDTPLKNDVSTIYNNGYEPEDETPVDHAYTIIGDHQFIK
                     TDGDTSKALSGAKFVVKNKIDPNKVTYLKQDPTTKAVTWIADKSKATEFVSGSDGKFQ
                     IEGLQYTYDVDFDKQGNPTITDKKINDYALEEVVAPSGYTPTRNDVPFVVSNDDASSE
                     VTDIKNLKTPETVRTGGIGTVVFLVVAGVAGTGLYVVYKREQHKA"
     gene            17746..18603
                     /locus_tag="I0B46_000813"
     CDS             17746..18603
                     /locus_tag="I0B46_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAR2083134.1"
                     /translation="MKKKNIKLIILKIGMLLCLGVGLYPVITQAYTYYQTQQTLNSYK
                     EKYDEASEKENEEVFKRYSEVNRKRKDTEQTSVTIPEDNNNIPSNGNLQKDKLGELVG
                     EILIPKINVDLPIYDGVNDFQLSAGAGILTGTDYPTGGIGNHSVISAHSGIPNKKYFT
                     DLELLKLGDKFFIDISGKKLAYQVDRIKTILPTEFEDLLPVAGKDYVTLMTCTPVGDN
                     SHRLLVRGVSIPYQKSIVEKEKKKEDLKVKAQMNWYPIVVVGVLLIIAVIVIIKKIVA
                     KRRGMKNEE"
     gene            18593..19702
                     /locus_tag="I0B46_000814"
     CDS             18593..19702
                     /locus_tag="I0B46_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAR2083135.1"
                     /translation="MKNKILLFLTLICTLVLTIYPANQLYHQFKTKENVAEIQEKIEK
                     KQTPSKEEVAKKVEQIKSNSGNQGLDNTQKTDKGVDNTQETDGGSFDIREAEGKVNPY
                     TGDVFSKNQTKDFEPSTYSGAVGYVYIPKINVLELLFLGASQEHLYRGVAQMSATSLP
                     LSGKGHQTVIAGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRTDVIRNYGQ
                     NSTLTDIPDLDMLTLYTCHPYPQNYDRLLVRSLRDEKAKYITPQVTVGNTAYADAIDS
                     ESVSDKPETVTSSNDLFEDVDRANKELDGNNDLNIPEQGSVEMQDRTFAEKASSIFTG
                     ITNKHQKMLLVIWTVLGALASLKVLILLIKEFRKA"
     gene            20041..20841
                     /locus_tag="I0B46_000815"
     CDS             20041..20841
                     /locus_tag="I0B46_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083136.1"
                     /translation="MVEISKTALDSLAKLFSSRFDGAEFVEYLSQNESGKTLIETLKK
                     DYLNENDFPTWTNIEDATWDVVCTIYSPWVTIADFVLDKIGGDTYPDAKSLATSDFKT
                     FYGYLMGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYADDREKVKLLEGKLSEEELGK
                     MVQEINEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDG
                     YLRSYSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
     gene            20929..21735
                     /locus_tag="I0B46_000816"
     CDS             20929..21735
                     /locus_tag="I0B46_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083137.1"
                     /translation="MRGDQTMSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWIS
                     AVGEENIEFEIKNMDDLKAKFPFIASKLSQFAEEKSWNELVSFSEKIYDITQEDECVS
                     WEMNNQYKKIDAFVKSRIEGTAFEFEGIDTADNWLNKESFIKSIADDLEKQWSKEDYA
                     EYLKTERDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYIVEEALS
                     QYSSDEEVDEETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLLIDQNDIE"
     gene            21967..22836
                     /locus_tag="I0B46_000817"
     CDS             21967..22836
                     /locus_tag="I0B46_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAR2083138.1"
                     /translation="MKKKKFSRSLLGILVLVAVGLSGKGYFQNNSTQEHDFQIKSSVQ
                     VGNKADSESKEFLGIRYDPNKYPNNIVNINDGKYTLTKEDHEALSEKGSQTFWVNYAD
                     LDQLGRAGDVEALITYDAINSHSSKNVERPSFKSSVHIAGEYKDGVFDQERDTWVGKD
                     SNNEITQLEGYKGYLYNKSHSLAWSLGGDMEVHNLTLGTRAQNVGKNDQNGGMAYSET
                     IVRDAIYKNKNEKVLYKVDPIYKGNELVPRGSHVEAFSVNDNGKTVNLNVYVFNNQKG
                     ININYQNGTWVRD"
     gene            22856..23203
                     /locus_tag="I0B46_000818"
     CDS             22856..23203
                     /locus_tag="I0B46_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083139.1"
                     /translation="METISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMIIEREMLDEIRVIIPKDTQMCE
                     DSISDDEPKSERK"
     gene            23216..24478
                     /locus_tag="I0B46_000819"
     CDS             23216..24478
                     /locus_tag="I0B46_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083140.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVASFSKSVYLVGRALVVGAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATSGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETERATSSIAVSNSQFSFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            24558..25934
                     /locus_tag="I0B46_000820"
     CDS             24558..25934
                     /locus_tag="I0B46_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAR2083141.1"
                     /translation="MAMKLLEKVKKRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKD
                     PTDAIFGIKPDGTIAHFCISEMPHMLVAGTTGSGKSVLLNEILVTAMCHSTPDELKIG
                     IVDPKRVEFGRYKKLPFMLADPITDMDEAYDFFEYLVILMHERYKLMEKAGLQNITLY
                     NEYAEKNGLDRFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMIIKLVNGSMVRAQG
                     AYISNKEIETIFNHLRNTMPEPEYVDYRAIVAESRGEGGEDEDFGGIGKAPVRKSAKR
                     NLNTPLANSSSKEPSIEKKSTFALKLEKNKEEKEKQGAKTLEKLQAEKLEKVLKALDK
                     ADSEPQTSNNTNNEQPISSKTKPKTTDPKVSVNVTITTDKSEKKQVNGTTSPIMQKML
                     KAKKQVHK"
     gene            25931..26560
                     /locus_tag="I0B46_000821"
     CDS             25931..26560
                     /locus_tag="I0B46_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083142.1"
                     /translation="MIIVSSFLRWLTPYKYTDEVVVKRTKKRDKKAVLTPKQMLDHGI
                     LGVKVLEVNKPFESNSAREKLLGAEADVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLRPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSNQNRVQINKFEKKVRK
                     FNDRKTILWFADICKQPDGTAKWEAVIFDENGKKVMSETWHDKAKFVWQ"
     gene            26579..27058
                     /locus_tag="I0B46_000822"
     CDS             26579..27058
                     /locus_tag="I0B46_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083143.1"
                     /translation="MLELFEANRTGFNQKLEVGETIEYKDKNYIIIGIYNTRINRIGD
                     PRITSDLVCQSVNYSPDLTSRYKKYVLLEYRHKITDEIGVNNTRNIFKIGTVIPYSNN
                     EEKIFYQIYGIEKFEYEHVDLLVTYQMRLIEPWSQAEIDKAVKLNRLSKFNVLGNAF"
     gene            27560..27613
                     /locus_tag="I0B46_000823"
                     /pseudo
     CDS             27560..27613
                     /locus_tag="I0B46_000823"
                     /inference="COORDINATES: protein motif:HMM:NF019230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            27617..28216
                     /locus_tag="I0B46_000824"
     CDS             27617..28216
                     /locus_tag="I0B46_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAR2083144.1"
                     /translation="MREKNKNSSRYLVATVVFVCVLIFLFDFLQIGKSIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGFLGSLVPFLLHSNIVSMGLSGGIFGWIALFAIAYIRFDSKKHLLLSVFVLLIIGIL
                     LVSTFESGETNVLSHVIGFVCGMILGFILKNVSLIKQKN"
     gene            28232..28708
                     /locus_tag="I0B46_000825"
     CDS             28232..28708
                     /locus_tag="I0B46_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083145.1"
                     /translation="MPKKKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMYE
                     QREALRERIHYFDDIHTKIYEARTNHEDESKLGLAQQRIFEWLNTNTEQEPLRAISNL
                     FEFGTKHWFNAVEKDADLQEAFDTLSEDSKSMRDLLITYLNEHEFEQTKVKKKNKK"
     gene            28725..29627
                     /locus_tag="I0B46_000826"
     CDS             28725..29627
                     /locus_tag="I0B46_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083146.1"
                     /translation="MLFTLLGYLCLVLAFFGAIAIFVGQFQENNPIMRNIGIGIVIIS
                     LIVFGATKIFGSNDQQFGISGVDNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDDK
                     KASDDVLATIKPSQSQFEVFYTVKENNPVRKLTLVFNVDGEEYYKDVKINKASTKKIV
                     ESAKKEVATEESTTESSSTVEVELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQRINVTLFNAVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGSDLPVH
                     VVYEDGKVFGDSTANEALTIKLAK"
     gene            29700..29894
                     /locus_tag="I0B46_000827"
     CDS             29700..29894
                     /locus_tag="I0B46_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083147.1"
                     /translation="MVVNKEKVRKYVTLKIASVEYLEKRAKEEEKTRGKKVTVSELLE
                     ELIESDQTIRRAFTNRSVKS"
     gene            29999..30427
                     /locus_tag="I0B46_000828"
     CDS             29999..30427
                     /locus_tag="I0B46_000828"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083148.1"
                     /translation="MIIQKFDWKMKLGISLVILGFIIFLGIKAQKNQSTFETMSSQII
                     EECSKIYYCENKTEESNGFYYIDQPHDVVIKMESHEVNEWIQRSTNVSKKLERYSQTF
                     LSKVIFSNCKNEETSQRVASSLENLKFSVSKKEKFVDLRK"
     gene            30662..31249
                     /locus_tag="I0B46_000829"
     CDS             30662..31249
                     /locus_tag="I0B46_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083149.1"
                     /translation="MENNNDIRYFSIGERVTTVSELSKDYRDKEGTIISLKKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNDGSHSIEKAILVFPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITNKEAKVEDVLTVRELQSLIEYRNKQITNPEYLKKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDNFKKIYLKEIEKD"
     gene            31249..32148
                     /locus_tag="I0B46_000830"
     CDS             31249..32148
                     /locus_tag="I0B46_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAR2083150.1"
                     /translation="MWSVTLPFQPVSAPRPSARRNVADATNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQGLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            32148..33062
                     /locus_tag="I0B46_000831"
     CDS             32148..33062
                     /locus_tag="I0B46_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083151.1"
                     /translation="MNNNEKILHVLDSFETIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLVERKEQEKLIKAYFELGEKEVKKAMELNEDRRVLDQLLEQLLVMFQ
                     KGRIDEDLIEEGLRKYPANSGIGIVLKAIDEEEIEDFIPAEDFESAMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCEGYEVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     DARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNASNRLEKAVSELDA
                     YYREHYLQAGGMPRNTKANISRYIKEAE"
     gene            33114..33329
                     /locus_tag="I0B46_000832"
     CDS             33114..33329
                     /locus_tag="I0B46_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083152.1"
                     /translation="MKRDVFELYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NSVVLQQFWLNNFSYKDFIKEKANRLY"
     gene            33519..33773
                     /locus_tag="I0B46_000833"
     CDS             33519..33773
                     /locus_tag="I0B46_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3034 family protein"
                     /protein_id="HAR2083153.1"
                     /translation="MKKEELIDMFQIVERANNMGIMFFDRISLKMDLSVAHQEFNLRL
                     KALLISDDVNFAHDVVGIQNHIDRENKRMGDGFLPRYSSL"
     gene            33952..35001
                     /locus_tag="I0B46_000834"
     CDS             33952..35001
                     /locus_tag="I0B46_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyhydrolase"
                     /protein_id="HAR2083154.1"
                     /translation="MKKFLVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGSPVYRLYNRYNGEHFYTVDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNVISKQGWKIEGTGWYAMPNS
                     APNPTPAPAPSNEKVLNVPVIWQGNTMLCEGASLLEGLHYKGVATNQNLTSFVNYMPR
                     ANDNNPYHGYSGEWRYNVNGTYQGMMPDPVVKWAKNVGGNAVNITGSGTNGIKNEIRN
                     GNPVVTWITYNYASPQFKQMPWGRAVWNGHVVLVDGFKNGAYHVVDPVFGAKWINAST
                     YERAFNVTGMAVAVR"
     gene            35178..35408
                     /locus_tag="I0B46_000835"
     CDS             35178..35408
                     /locus_tag="I0B46_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083155.1"
                     /translation="MNQRRRKRLEEISERLSELKDELEVIQEEEEEYKENIPENLQGS
                     ERYEKAEESVDQLEEAVDGLSDILELIEDIAS"
     gene            35411..35668
                     /locus_tag="I0B46_000836"
     CDS             35411..35668
                     /locus_tag="I0B46_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083156.1"
                     /translation="MINEDFRSEQKMIDISLIEKLLFSNKYTAKELATRWQISESAIK
                     AYRQNKGSVGWRDWKGISIGKAEDIMKREGFQADNDSKKVE"
     gene            35885..36760
                     /locus_tag="I0B46_000837"
     CDS             35885..36760
                     /locus_tag="I0B46_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083157.1"
                     /translation="MTTNKRWSELVQNTVKKVMPDGYHFNYIQCSQTTESIYFSILNE
                     NRLYYFRVSSHHRKKREDNVTTFLSGKYNGFKELSEAIGEYLKSNRFVRISDKHILIL
                     LLYLNNSFKLFWRDDTFYYIENGMDIYSGINGILKHYIKALLRYGVLYQEDLSNQIKI
                     SNSGKSLLAKYKKLLSPIYKGIDMEAVNSETLFNLVVDEKFANEFFKSKKIERLNNLF
                     PENAIKAYVDNNKSRLYNWFIPKRLRGKIKIGDQVKVINLNHTKIVTVTELYIAEKED
                     ITKMKPVIKILDNKE"
     gene            36770..37564
                     /locus_tag="I0B46_000838"
     CDS             36770..37564
                     /locus_tag="I0B46_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083158.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSRRLRITVEYGTNESATELKQRYLDTRREASKKYDDFKSRGLIPK
                     KKKGIWREFFKEEDSNRNKGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNTL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLALAKKARDLRETQ
                     MYLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMNKYRV"
     gene            37593..38711
                     /locus_tag="I0B46_000839"
     CDS             37593..38711
                     /locus_tag="I0B46_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083159.1"
                     /translation="MGNNSIYTPEMQNKIKRLVGLGWMTRGKLDSFSTIKYLLMDIYR
                     QNKQTGIHFLGYDKPEDAPFFVPSSAYRASTSGSMYNLDLFEEGAKMIEYDFNEAYSR
                     IMRNYPLPSNTYLKAKYNTDQAILRAINRPKVRPYENLKTYMFFLVNFEGTAKEGTYA
                     NPKSQSMFADYGENPKTANEPIWLSEIELTLILDYYDIKEMEVLDSHTFLTKKGLLKE
                     YFERIEPLKTDPDTLKLYKNLRTKVFGAIGQKSLREGETSTFDKYSMYNRAYSSMVAG
                     VFRDRMVRYEQKYVHSAYGLLKIRTDGLYFRSEVPEFEELYKLGVVKKKVHTISKEEV
                     EKALEFKNKGFENKGSFGKSKDEVKGVVIDDDFEFDFD"
     gene            38744..39064
                     /locus_tag="I0B46_000840"
     CDS             38744..39064
                     /locus_tag="I0B46_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083160.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEIFFIEEWEGTENLVLHQY
                     LLKA"
     gene            39319..40008
                     /locus_tag="I0B46_000841"
     CDS             39319..40008
                     /locus_tag="I0B46_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HAR2083161.1"
                     /translation="MRTHYIYLEDKIVDEIPAKSREQARKLFIEKYKEINGDSEVPKV
                     VVTVRKRQTKKRSTFHVSPDIYNLIERHRSEDNISISQYIATVIDELISGKYDYAFDF
                     EGEKDYKSVAVFQSDFEKLKNYAGRRNVNFARLLEEFYSEELGVGNEYKRETYAKLSH
                     KLSQALKEVTEVKAELDAKLEEVKKYQTERKNNMKNQVEFDEQKEFEKARKRYNKIMN
                     ELSYEHLTVHE"
     gene            40045..40212
                     /locus_tag="I0B46_000842"
     CDS             40045..40212
                     /locus_tag="I0B46_000842"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083162.1"
                     /translation="MVNEVETLLASFFENGHVNDEWRKEEPKRVNAYIARLKRFIDKY
                     KENSDKFSKDK"
     gene            40232..41860
                     /locus_tag="I0B46_000843"
     CDS             40232..41860
                     /locus_tag="I0B46_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083163.1"
                     /translation="MPEIEGIFSPKIGKKQLDFSSFPSVNRDYNLGDEVQILNIDFKE
                     LDFAVAVSNKPKALERVKKIYQENDQNIKNMIKKYRRDNSKVEDVLFNGTAKHDLNVY
                     MALGVALYCNSINQTDILVNIVRNSYQKILKISSETNPYYAMKYLGDNLLNDKKLMHN
                     TEELLSNLAAINFLFKTGSEDYFIDNVLKGAYGTAFYPLYNMKQHKAEQKDNKLEIKK
                     MKELLGIGFVKPNSKVTIASLATTNNDGKIVNGFDDSDIDILSKSYFSLYGDFADLMG
                     IDSYLVNNFEIKGSELNDIVYFVLISLYAIDQNTEYRQIDYLLVFYSLLYQFVLTRLF
                     KNAKEELILNSDYEIAKQIASIRKENKSEVIKLRKELSIASKENGRLESLVKSAKKEE
                     ELLKQEIKELKGIRKELEQLRFENKVLKQLQEQEHRQSLNGKTEEPSLSEMIAYLKHK
                     NILLVGGHVNFQNKLTELLPNIKMFDVSENNGKILKNLNTCDAIFLYTDYLNHGLYYR
                     VMSEISNKDKIFYLDNHVNMNLTIKRIYDLLSLI"
     gene            41911..42282
                     /locus_tag="I0B46_000844"
     CDS             41911..42282
                     /locus_tag="I0B46_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083164.1"
                     /translation="MRELTLSSKELEKKTALLNEIFSADCIIQSLKESSFSKLTDQEK
                     ISYMLSLDNLGTKYDESASSDKLLIELVSSLSSFGKMKRFNVILEIFAWLIPSFIWFF
                     YIFIVESELIAKVSLGLLIPI"
     gene            42367..42567
                     /locus_tag="I0B46_000845"
     CDS             42367..42567
                     /locus_tag="I0B46_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083165.1"
                     /translation="MLKRIAWDKDNKTDIELKEYKYNYFSRCIDRKLLFKKEGLEFSF
                     DEDEFEKLVMDWRKNLLDTVQF"
     gene            42701..43927
                     /locus_tag="I0B46_000846"
     CDS             42701..43927
                     /locus_tag="I0B46_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083166.1"
                     /translation="MIFYMFININEVLKLTTITMTKKIEVYVDGSQEERDSFFDFFWK
                     NSREYVKALNLVYKQLMFEELIKDAIKEMDQGYAEREESIQNRIEKLAYTDPIDQKKL
                     DKAKAELSKLRNEKNKEAREILTQAIGLKSQTRTRDVAKKLDFDFGDFVDSANMKASK
                     DFGNDFIGIVTGQRTPRIYKNRNKYGLLPFRGRDIKIHKDDKFYIYLPKGFRFKVNLG
                     SKPRKARDVASVLQHIMDGDYKLNQSNLIFDGRKLFFNLTISFEKSYNEMEFKKGNEL
                     IVNFADLCSGECLINDKSVMSFGDSQYIRNFKSKILAMKSKEQSRGVFSKGGHGRKRK
                     LDQDRWEAIKKRESNFVKTYNNQCSAKIVKTAIRNKAQIIRIIEPDKAYGEWAYYQLK
                     EQLVNKGKRYGIEVRS"
     gene            44777..46102
                     /locus_tag="I0B46_000847"
     CDS             44777..46102
                     /locus_tag="I0B46_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAR2083167.1"
                     /translation="MFDYSREPVNDYFLIDMKSFYASVECIERNLDPMTTPLIVMSRS
                     DNTGSGLTLASSPTAKKKYHITNVSRATDLPYPLPKELHIVPPRMNLYIKKNMEVNQI
                     FRKYVADEDLLIYSIDESILKVTKSLNLFTSSGTRSERRRELAKIIKDEIQKETGLIA
                     TVGIGDNPLLAKLALDNEAKSSKEFIAEWTYETVPEKIWKINEMTDFWGIGSRMKKRL
                     NHMGIMSIEDLANWNPYTIKSQLGVIGLQLYFHTNGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPKDYTRREEVELVVKEMAEQVAIRIRQHNCKTGCVRLAIGTSILEEKSGFSHQMKI
                     PITDNTKELQNYCLFLFNKFYEGQEVRHIGVTYSRLVYTDSLQLDLFSDPRKKIAEEN
                     LDRIIDKIRQKYGFTAIIHASSMLEGARSITRSTLIGGHAGGLGGLHND"
     gene            46095..46442
                     /locus_tag="I0B46_000848"
     CDS             46095..46442
                     /locus_tag="I0B46_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083168.1"
                     /translation="MIEYNDRGRLKWSGFYLSDHTERINADNVQRANHNLAKEQMTTE
                     EIAKILRTAVIKNRPVCIQKEERNAEGYYPDDVVGKIIGYDELGVFVEEEKVHYDEIR
                     NVSFSELLKWSQI"
     gene            46793..47614
                     /locus_tag="I0B46_000849"
     CDS             46793..47614
                     /locus_tag="I0B46_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAR2083169.1"
                     /translation="MGLLVPRANTYTIPLTKRQLIAKRYQRITRAINREFWNSESDTA
                     HSLYVGSYGRGTAISTSDIDIIVELPMAEFDRFKNYLSNGPSKLLQVIKNAFQEILPN
                     SDIRADGQVVKINFHDGIKFEIVPAFNEKDYWGESKGFIYPDSNMGGNWKATNPKKEQ
                     EAMKLKNTKSNNLLYATCKHFRHVRDTEFTSYHLSGIVIDSFVYEAMGNWKFVENNSG
                     GQNISSVSYETALLEYYNSHKVMGGLNLYSPGSNQFVNSDSSIICLEKVLKKIAL"
     gene            47634..48194
                     /locus_tag="I0B46_000850"
     CDS             47634..48194
                     /locus_tag="I0B46_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HAR2083170.1"
                     /translation="MGDEKKRLEVQLREAYGRVTYTYTTHLKQADNIISDNKILRCLQ
                     IGLSALTTGGVLSTIITDNSELTFVAGLCSTISLGLNLYFKNFDLAEEAKNHQIVADK
                     LWLIREEYVSLLTDLNRLSVEDIVKKRDQLMNETHEAYKNAPKTNSKSYESAQKALKS
                     EEEQFFTEEELDKMLPEHLRKKSSKY"
BASE COUNT        18273 a         6488 c         9536 g        14096 t
ORIGIN      
        1 atagatgaga atggacaatt ttttgtttgg atgcgacatg agcattgttg gtgtaatcct
       61 tttgtgttaa aaaaagaaaa tgaaatcaga actaaaattg caaatggtga gtgggattac
      121 ctcttcagat gggaagatga atcttttata gcaaatgata tggatcatgc atttgtctca
      181 tttctggata atacatgggg tacttggtac gattgttacc tagaaaaatt gggctttcaa
      241 aatattgata ttgatgctat tgaatgtctt aaagaagtcg atcaaaagta tgaagaagaa
      301 ccctattatc tcttgcaaca aaaattagcc aataattcag tgtttcaggt agaaatttgg
      361 gaaaatgaag atggaactta tgaggggcta gcgcaagaaa tacccttctc agataggaag
      421 agttcgattt gaaaagagta tatcagcaat ttttatgaag tgaaagcaag cttacaatat
      481 agtaatggaa aattgtggtt agctggaagg tatattccag ctaaccaaat cttacgtttt
      541 ctgggagagt ggcgttttaa aacaatttcc taggaaaaat agttaacata aaaggaatga
      601 attgttatga aacaatattt aaatgaatgg aaagcgatag aggggtcatt agtaacgcaa
      661 agaataaagg aattacctga ttgcttagag aaggatcatt tgtttcaaat acgagaaatg
      721 ttgaaaaaag aacagtttga tccagaccaa tttttagtag tcgagtatcc aactattgga
      781 gtgtactgtt gtaaccatat caatgatgga aaatatttta ttattcagga gtatggagga
      841 cagttaactc ctttctatac tacatgggaa atgagtgaag aaggaatcaa taattttcca
      901 tgtgaaagca tcgaagattc aatttcacac accgaatgtt aattgattat aactataaaa
      961 aaggattgaa cattatgttc aatccttttt tcaatgaaat gttttccgcc atttcattgt
     1021 ttggtgcgat ttctatttta ttatatcaaa ataaagagag attctaaaga aagaagaggg
     1081 aaatatataa atcaagcgac agtatattta ttattaaatg ctattgtgag aatataacta
     1141 attagtttaa tttgaaagta aatatacgct ataaaagtgc atcttgtgtt ataaaaaatt
     1201 attttatttt gtgaacataa tttaacaatt ctttctcgga attgatttct tttcttaaat
     1261 agtcttcaga cttattaata atattatcta ttatgtctag atacatatca cttttcgatt
     1321 cagctaattc atacagtttt tttaattctt tatcctttaa ttgaaaaccc tgatccatag
     1381 tcactgcatt tggatataga agcccactat aatgtttacg taaattcact tggaatttct
     1441 gattataaaa gtctaacaga ttagaaataa aaggcggaaa attactgttt ttgtaaaatt
     1501 gattatttcc atttaggttg tatagtaatt tgttaaattg ctcctgattt aatttattgt
     1561 aaatccatgg taatacttcc atatccttat ctgctaacct gaatgaacta gcatcggtaa
     1621 gatgttcgat cataaaatct atgaacatct ctttttcacc aaaatataaa tattgattat
     1681 atattgtttc taatgttgaa gatggtagcg aagaaactct cattttttgc tggagtatag
     1741 gatcaactag actatttaag tgattttgta attcttcatt gttgtttcct agaactgcaa
     1801 gggaacgtaa acggtagtaa tattctattt ctgttagtac ttgttgtttc ggtttaaaat
     1861 catttattga tatttgtaaa ttttgaattg tctcgtctga aaaatcatgt tgtaattctg
     1921 gatgggtagc tataaaagta agtaaaaaga acccttttgt atacgtgatc tgttgatttt
     1981 caccaagtga tttattaatt tcctctcttg attcagctgt gaacctatac tttgcaattt
     2041 ctttttttag aatatttaaa tagaattctt tattatcaaa aattagggca ttaagtagtc
     2101 tgaaagtact ttccctattt ctatctgctt cctcatttcc ataagataat tgaaaaacaa
     2161 acttaaaggc tgtcttacat aggttttctt tggctttttc gcttagttta tttagatagc
     2221 tattttttag tgcagagtga aattcttcag gtgtaacaga catagcatgt atgcgacttg
     2281 catattgtat aatttcgatc gttacttttt ttcctaatat tggatttttt aggaatataa
     2341 tttcatagga cttccgtata acatcctgac aagtttcttt tgttatatct cttaattccc
     2401 aagtattacc gttgtaagtg actacaggat gagctgaata atttctttga tccttaatat
     2461 attttaaatc ctcatattca tagctatcaa ttagctcagt tgaactataa gctttttcta
     2521 gtagttccat ttcccatttt ggagagtttg gatcactttt tcttagtcta gctatatctt
     2581 ctaaaatttt cttagaagta ttatcctcgt aaatatttgc taaatcagat aatttatcaa
     2641 ttaaatctaa aataagcgca ctgtaattga cagctattgc agaacgataa tttccctgat
     2701 aaaaactact tctaacttct tctaataatg gaataattct aggattttta acttttttta
     2761 tttcatcatc taatataaac atataaatct ccttttttta tttgtatgac aagtttacca
     2821 taaaaaaaaa ttaaaaagcg agacatattt atgttgttgt gttatcataa taacataaat
     2881 atgtagagtg agatgatgaa aaaatggatt tgaaacaatc gttttacatc tggttatatc
     2941 ctgataatcc tatgtcaata atggctgata tttttgtagt tagcatctta ttgtatttat
     3001 tttatctaat tttctttcac aatttaatcg ggtacatgat tcttgattgg aaaggaatgt
     3061 tcgatataga aagaaaacca tttgctaaca atcaaaccta taccgtcttc gaaagtggag
     3121 attgggaaga aaatgaagtt gatgtgatcc atgaaattgg attagcttta tcaggaatac
     3181 aagatgctga tgttttggta tcaatgacaa cggatactgg atacaacgct tatgaggtat
     3241 tttatcttat gtattttata tggcgtgatg gtcaagaaat tatcaatggt cgtgctgaaa
     3301 aaacaatcaa ttctaatact gaaatttctt tttgtatggg tgaaggggaa atgattgtat
     3361 ataaagatgg gtcagcaaac attaaaacag cctttggtga tattgaaaca agtattaagg
     3421 aacttaggga atatgtgcag aacccatata attttcaata tttagcagga agaataaatt
     3481 tagtttagga gtaaaaatat gaaaaaaatt ataaatcaac caacaaatca cttgataatt
     3541 gaaggtgtta ttttcgtaac aatgtatttt actgtaggaa atggcatctc aaatacaatc
     3601 attcaaaaaa atccacaata cctattattt agtctttatg cttggattgt acaaatggtt
     3661 tgggtttcca tattgatgct gttgttttat tggatagtac gggttattct tttattgtta
     3721 aacaaaagaa aaaaggatag gtgaataaaa tgaaatcaac taaattgaag gttgtattgt
     3781 tatcgacagc agcattaata acccttacag catgtggtac aactaatagt gctaataata
     3841 acaaagacac aaagtctagt agtacgatgg ttactaaaaa cagcactaca tcggcatcaa
     3901 caacaatgtc ttcaatgact gaaaataatg ctgaaaagta tcaaaaatac gaagacatta
     3961 ataaaggtca aaagccggat gaagtttata acaactttat taatacagta aaaaatgatg
     4021 aaaattatgg gaatgatcca ttgcaaaata aaaatctgac tatcgttctt tacaaacctg
     4081 attgtccaga ttgtcaaaaa gcagaatcaa aaatcatgca agaaataaaa gaaggattta
     4141 gttatgctcc aacattaatg catgatatta ccataaataa accagcttac gagtattacg
     4201 cagttgctgt agatattact gatggtgtac ctgagtgggt acaaaacatt cctggtctgg
     4261 ctgatgagaa caatcaatat catacgccaa cagttgcagt agtaacacca aagaaagtta
     4321 acggcacact atactgggat gtaatgggaa tgacaacaaa tacaactgat gaaatgattg
     4381 aaagtacaac taaatattat atggatttgg gagcatataa attatatcaa caatcagagc
     4441 ctgttatgga cgatcaatcc aaagttaaat agatctttag ggcgtaagcc ctattttttt
     4501 aaaattaagt gataacaaat aaatgatttc tgaaacaaac gttcttactc aactactcga
     4561 aacacacaag ctaattaact agattataaa ggagaatgcg taatgaaaga aatcagtgca
     4621 aatgaagctc atattccgca aaagaatcag gttggtatat ataaggtgct tcatggtgaa
     4681 ggaatttata aaatacagtt acttaccggt tacaataatg gtgcaaatgg gaagtggtta
     4741 aaggcaacaa gtattcgtga acttagaagt gaagtagaaa aaattaggct tggtctcaca
     4801 agtgaagaaa tagctaaact gaagggaaat aattcttttg taattgaaaa agaaaaggaa
     4861 aaacatctac tttatcgtga gctgaaaatt gaagaatact atgaaattga tttacctgaa
     4921 aaattaagaa ttattgatca acgttcagaa gaaaaatgga aaactgaaat atatccaagc
     4981 ttaaatgact acaagacatc aggaaagccc atgtataact acacaggctt tttaacctac
     5041 acacaataca aatatgcctt tcgcaaatta aaaggcatta aacaagctga aattgctctg
     5101 tatgagatga aaaaagtgga tagattgaaa ttgattcaag atttagtgca aaatacatgg
     5161 aatcaagcga ttgtcgagct agaaaaagaa aatgggaaaa ttttaactaa taaatatctg
     5221 atcgtaaatg aaattgaaga attaattggt ttattttcat gtgaaaaagg tgaagtcttc
     5281 ttgaagtcat caccaaccta ttcaattttg gacagtccgt atcagtttat acaggcaagg
     5341 agatgagtta gatgtctagc ttgaaagatg actatatcca agcaaaacaa gagtatgaaa
     5401 aaatacagat gttgaaatct gagaaagtcg aattattttt aacacaaaaa ttagcagagg
     5461 atcatattgc taacttagat gctaaaactt tagaggaata tgatcggcag tttaatcaga
     5521 tacatcacaa tatcattttg cttgttgagc aggcaaagcg aaggtttgaa aaagcggaat
     5581 atgatctatt agatcaagga attgtatgct tggataaggg attgcgaaga tattatggtg
     5641 cagagttaaa aaagcaaaat gtagatagaa atgcactaaa acatgaaatc ttagaattga
     5701 taatgcaacg aatttgaagg gatagaaagc aaatgtcaca aaacattagt gctgtaaaac
     5761 aattaataga agaagattta gagaccacag ttcagagatt agcggaacga atccattttg
     5821 caaattatca aaccatctct aactggtcaa gcaggaacag aaatccggaa gaaattccaa
     5881 tgaaattttt ttatcgaatc ggaaaagaat atggtgttga tccgataaaa attttcgaga
     5941 agaccatgat ttattactat catggaacac ctaacgaagt gttagaacat cttttggaag
     6001 aggcaaaaga aaaatcatca atattagagc tatcgcaaaa tgacaaagtt attcaagtga
     6061 aatgcgttga tccaacagga aagattattg tcggttttga tattgaaaga aaatccaaaa
     6121 tagattttat tacacaatta aaaaagataa ataatttaga tattctaacg cttagaccag
     6181 atcatgaaca gttgtttatg agttttaaaa aacaattgaa agaaatacaa aaaagatata
     6241 acaaggtgtt tcctaatgcg tgaaaagctc aaagaaatca gagttttttg gataattaac
     6301 aaagaatgta tcatcttata tactcttgga tttcttagca tgatcttgtt ttggtggtta
     6361 tatttaaaac agtaattgga gagggtcaag aggattttta tcaagataag gatgtggaaa
     6421 ctgcatgtgg aataatagat taaaaacagg attgttatta atagttattt cctgcgctat
     6481 gatgattggt atgaggatac aaagggaaca atcctatttt gaagtttctg ctaacaacgt
     6541 aatagaaaaa tgctattacg gtcaacatta ttggagtgag gaagtcagag aaaatataga
     6601 tcgtgaatat gttcaaagaa ttgttagatt accagtaagt gaactaaaaa aatgatgaga
     6661 ttatcagcat atcttttaat gtttttgaat gtggtcacgt taatctttgt agtaatcggt
     6721 gtgactttcg atttaatgga atagtgggaa atctataatg gaaaaaagag acacgaatga
     6781 acatattttt tgaatcaata ttgttgatgg tttttgcgat cagcgcttac cagaaatcta
     6841 aaaaagagta atgattgttt aatcatcact ctttttttgt agggaaattg aaatatgtgg
     6901 agcttgtatt ggtttagtta ggattgcttg gctataagga gctagttctt tatcaaaacc
     6961 atcaaagtag gttttcagta gcgatttaac tgatcgctca tcttgtttga tttggataat
     7021 ttcagcatcc aaatcggttg tgagatcagg agcttttgat gcgattacct gatttttttt
     7081 atcttccaac tctcttaggc tgtttaacaa gttgtaaaaa gaatcatttt gattgttcat
     7141 tccaacgtga gttgcaattt taacagaagt ttcgtccgta ttgtcatcaa aagaactcat
     7201 tggaatatca agtagtaacg aggatgtttc ttttactgac tttttcttta cagcattttt
     7261 tgtttttcca tcttttttgc tctttactcg agatgttttt ggagctagtt taggtaaatc
     7321 acttgtttta tcagtcattt attagctttt cctttctatt aatttatgtt attatattat
     7381 cataataaca taaaaaagta cactttttgg gggcgtagtt attgaaaaaa tctaagagaa
     7441 agattgccac attgcttatg ttaatggtgc aatttacagg gattgcaatg agtgtaatca
     7501 caataaatcc gcaagtgata catgctgatg ggatagctac ttatgatgca caatttaact
     7561 gtactactga aggtagtggg tcaggaacag atagcgatgg aaacgatact ggaagtggcg
     7621 ctgtaacagg tgatgtagcc aaaaatattg aagatattta caaatacatg aatggagaat
     7681 atggcttatc aggtgctagt attgcaggta ttttaggtaa ctggaaacaa gaatcaggta
     7741 ttgatcctcg agcagtagaa gggtattacg gagatggaca gagagaagtt gacttcatca
     7801 aacaatttac tgatggtctg atgggtattg gctatggtca atggacagct gggcgacata
     7861 cagcattagt tgattggtct aagaaaaata atggcggttc ttggtggact acagaagccc
     7921 aattggactt tatgttcaaa ggtgatgggg gattcgtagg aatattaaaa gattatgctt
     7981 taaatgcttc tgatgatgtc gtagaaaatg cagtaacttt tcataaaaac tgggaaattt
     8041 cagctgattc cgaatcagct gtaatagctg tacgtggtga aaatgcaaaa gaaatctgga
     8101 agtacatgaa aagcaagggt atggacggtg caaaagatga aagtaagatc aataaaatat
     8161 ccggaggttc aagtaccgga gatggatcga ataattcatc aagttcagca tcaggaacag
     8221 tagaagatgc ctgtgcaact acggatgacg ctaattctgg ttctggatca attgggaaag
     8281 aaggaaaaat taacggtaaa aaaggtcaag taatagcgaa tatgacttat gaagaagctt
     8341 tgaaaaaata tgggaaagcg atcacgctac caaaattcaa agaaccagat tggtcaactt
     8401 caccttttaa cggaatatat caaggtcagt gtacagagtt gacgtgggct tatatgagcc
     8461 aattatacga tgggaatcaa ccgactactg gaaatggtgg ttatgtatgg gaaagctaca
     8521 aatcggcagg agcaaaaata acgaacaaac caatcgtagg ttatgggttt agtgcttcag
     8581 atggatggtt atgggctgtt ggcgatgcag ggcatactgg tgtagttata gcggtgtttg
     8641 aagatgggtc atttctatgt gctaacttta atgttccacc aacccaagct ccaacaaggg
     8701 gagtttcggt aactttgatt gatggagtgg acggatcaga taatgtgcat ttctttagcg
     8761 gtgtaggaaa agctaaattt gaaatcgaaa aataaattgg aatgaaggac gatatagatg
     8821 aatagtaaag tgaaagatac aattatcatt tctttgcttt ctggtatgtt attagcactg
     8881 cttgttggaa tcctattctt caataagcag aatcaggaca tagaagctga aaaacacagt
     8941 gtgcatattg aagataagct gacattcttt aaagaggtta aaccaaatga ggtggatgga
     9001 ttacggaaag atttagaaaa tgatattcaa gaaatgttga atggatcagt tcaaaataag
     9061 tttgcagatc aatcaggtga agaatttttg agaaaactat ttattgtaac aggtaaacca
     9121 ccaattactg ataagagtag tgagaaagaa aaaatcgagt actataagcc ttttactttt
     9181 aagctaacaa actttggaat taagacatct agtagtgggt tgtatgaagc attgaccgat
     9241 tttgaagttc aatacaatgg aaaaaaagtg tatgatacca cattaaccat cacactaaat
     9301 aatgataaga aagtgactgg gggtgctttt tatgcaaaat catgaaatca ctactgctaa
     9361 aaaggcgatc ggaatacttt ccataattgt gatcctttgt tgtgtggtac tatttcaaaa
     9421 gaaaaatgct tatgataata atgtcaaaga attggaagac cttaaaagta aacaaatcga
     9481 agtagtcaag aataaccaga aagtgaaagc tatgcaggaa gctcaataca aagaattggg
     9541 gaatgctgaa atcaaggaac aaagtaggaa attctttaat ttattttaca actggaattc
     9601 atgggaaaaa tatacgaaga atatgaagga aatacaaaaa gaatttcctc agatagtaaa
     9661 gtctagtcct gttgatattt ctggcacgat ggtaggcact ggtaatagtc caatatctag
     9721 ctatactgct gattactata caacaaatca aaaaggtaaa atggttgaat tgattacaca
     9781 gacaagaaat actccaacaa cagcaacaac aacaatttgg cgtggagtaa caagcatagg
     9841 agaaaatcaa ttgttttact tggaggattt agatagctat gtaagagcgg agtaaaataa
     9901 aaaaagagct aacggtacca acgttagctc tttgtgctga cgatgataac aaaacctacc
     9961 gaaaaaggat tgtttcatca caaacacaaa cagaaaagaa ttgataaaaa acccgaacct
    10021 cagaattttt attaatcaat tcatttagat tataataaat tgagctgaac attacaatac
    10081 gtatatgttg taaaacgtaa aaaaagtcaa aagtaaatac tttcttaaaa agtcaaataa
    10141 aactattaaa gacaatatat atggtttatt tgacataaga aaatggtcta gatgataatt
    10201 gtaatataga attgtaggct taaataaagc ataaaatacc ttttatgata aggtatagct
    10261 cttaatttta agcagttttt agatataaga gtgaaagtaa agtgggggag aataataatg
    10321 gcagatcgta aattagaaaa gttattagaa gaaacgtgga atccaaaaga atttagtgaa
    10381 ttttttatgg agaattttga aactgactta gcagtgattg ttaaagatgc tcttagagag
    10441 caagggtatc ctgaaactgc aaactatata aacattaatt ttactttgta tactgaaaat
    10501 aaaggaactt gggatttttg ggctacttta gcaaataagg agttaagtga taaatcagac
    10561 acaggaattc gtaacttttt tgaatcaaac agagatgatt atatgtatgc taaccatcaa
    10621 gataaattga actttagagt agagtttgat gaaacacctg aagaatttat agaaagacaa
    10681 ccgcctaaag aaaatgtagc aaaggtattg gaagatcgtt ggaattcaga tgaaatagtt
    10741 tcaacaatta gcgaactagg cggacaatat gaaccgctag tagaagcggt tagagaagag
    10801 ctaagactga ataagtttcc tgatgttcaa aatattgatg tttctcaaat tgaaataaac
    10861 gtcaagatca cgaataaact tgattatggt agttgggcag atatagcatt agaaaaatac
    10921 atctactcaa cgttaaaaga gtttatcgaa aatagaatgg atatcatgta tctacagcat
    10981 ccacagtatt taaactttgg tgttgaaata gctacacctc ttgaagaatg gaaaatggag
    11041 caagggcttg actagccttt gctccatttt tttattttac aaatgaatac agttcgtcgt
    11101 ttttaccaag aatgattgtt tgcccaactt tgagattgtc aggtgatcct tcagcggttt
    11161 gtaaggagaa cgttccgttg aacttatcgg ataccgttat aggtagcttt aattctgatc
    11221 cttcctcatc tttgagaatt gctgtttttc gttcatcaaa ataaacagtc tttattaaat
    11281 aatttttcgt agtgatctca gcagatttgt ctttttttgt agtagtaaca gtttctttag
    11341 aattagaggt attttctacg actattccat ctttggcatt tgagcttgtg tttatttgat
    11401 tgaatatttt cagcgacaag ataccgctaa gaatagtcaa tatagaaaca agtgtaatta
    11461 atactgataa tttccttttt ttgttcattt tttcttaact tcctttttgt tttgattagt
    11521 tagatattcc ttatctaaca agccttttta ggctattaaa tgaagataaa ccccaaaaaa
    11581 atattgcaat tatgacagtg caatggtact attattatac aataaatttt gcgaaagggg
    11641 taggtctaaa tcgcaaacaa aaagaaattg aacctagaca aatacaactt gtacaccgca
    11701 agggaagcaa gtttgatttt gggtaaaggt agaaattatg ttacaaacat aatcaactat
    11761 tatccaaatc gtttgcctaa aggttcgttc aagaaagtgg gtgacaaaac ctatattata
    11821 acggaagaag gaatagaagc attgcgatca caaacagaaa ataaaaacaa aaagaaggaa
    11881 tgaatatgaa gaaaaattcg cttggtatgg ttttatttgc atcagctgta cttgcggggt
    11941 tatcagtacc agtaacctca catgctgatg aatggactgc aaacacaccg gatcaaatcc
    12001 aaattacaac aggacaatca agttatgcgt tcaaaaaagg agatactatt tggtcaactg
    12061 tagagaagct taaaagctta ggttacaaga ctactgttgc tgagtttgcc gaattgaaca
    12121 atgttgattt ggcaaaagga gaacaacgga ctatcccaat tggacgaact ttcaacatca
    12181 gtaaagatac attaacagtt aaggaagcag atggcgaagt agttagtcaa actgttcaaa
    12241 aagataaacc tgatcctgtt gaaaatacgg agcaagagaa caaaaaatcc gagactgcaa
    12301 aagaatcgga accaacagca aacacttctg ataagacaga aacaagtgct tcaagtgttg
    12361 atggagcaag ttcatctaat acaaataatt ctacaagtga gaaagaagca tcacagggaa
    12421 caagtgaaag tagcactacg gaaactgata ctggaacatc aacaaataac tcacaatcaa
    12481 catcaaatga tgcaaatgaa tcaaccagta catcttctag tgatcaatca caaacaggtt
    12541 cgagtgatga aactgacagt tctacttcac caagtaaacc cgaacaacca actaatgttg
    12601 tgctaggcgt tccatttatt agtcaaggta acacaatgct ttgcgaagga acatctttgc
    12661 tagaagcgtt acattataaa ggtgttgcaa caaatcaaga tttaatgact tttgttaaat
    12721 caatgccact atctcctgat aataatccaa ataatggttt ttctggtgaa tggagacaca
    12781 atgtggatgg aacttatcaa ggtatgaatc cggctcctgt agtttcatgg ggtgttgcta
    12841 atggcggaaa tgttgtagat atttctggtt catcggtaca aggattaaaa gatgagattg
    12901 taaatggaaa tcctgtagta gcgtgggtaa cttatgcctt tgaacctgct caacataaat
    12961 caatgttttg gggcgatgct atttggaatc gacatgtagt agccgttgat ggatttaaag
    13021 acggttttta ccatgttgtc gatcctgtgt ttggggcaag ttggattaat gcaggatcat
    13081 ttgaaacagc atataacgta tctcaattag cggtttcggt acgttaataa aagagattgt
    13141 ttatatacta agtagttaag tatagtcttt atttgtcctt gcttaactac ttagaaaact
    13201 tgttgctcat ggtgggtaga actaaaaggg gtgatgctat gaatactaga gcgttgaaac
    13261 agcttgcttt caacgaaaaa agtgattaat aggtgtggtg attttaactt aggaatgggc
    13321 gcgaaggtca ttaccacacc gacatatcgt ggctcatttt cttcgcctaa ctggtataaa
    13381 gaaggagtaa cgagagatga agcatgaaaa aacgataatt ttaaaatcaa aaataattta
    13441 ttgttttgcg actttagtaa tggtgttatc tacgcttata atgccttttt cagcaataag
    13501 agctgaagca gatgtcacaa aaccgacaag tgcaaaattt acagaaactg gcgtaaaaac
    13561 agacggttat attagggtta atatgccgaa tcatccaaat gaatggatga tttcaagtca
    13621 atttaaagat tctcatggaa atattggata ttgtatggat tcagaattgc catccccaac
    13681 aggaagtgga gccggaagct tgaagtacaa gggagctggc agtgatgagt tctatcgtat
    13741 gtttaaaggt ggttttccat caaaaacagc aaaggaactg ggtgcaggaa atgatacaga
    13801 agcatggtat gcaacacaat tagtttcatg ggttttagcc ggaaacttta aagtttcaca
    13861 gattgtatgg tcacatccaa accataccgc cgcagaaaca gctcgtgtaa aaaaagcatt
    13921 tgaaaaaatt tatgactatg ctaagaatgg aaaagatacg cccaacactg agttttctat
    13981 cacagcttct aagacagctg acgaaggaaa atatcacact ttcacatata aaacagcatc
    14041 aaataagact ggtaatgcga agctcacttt tacatcagca aaaccagctg gtatgaagat
    14101 ttatgatgca gatggtaaag aaattactaa caacactgtg aaattaaatt caagttttac
    14161 tattaaagta cctgtgacta caccaagtgg tactttgtca tttaaaggta ctgctaatgt
    14221 tagcacaacc aatcctttta cttttgatgg acgaggcgtg tatcaagatg ccgtagttat
    14281 gatcactact tcagaaacaa aggactctaa atctttgagt gctaaatgga caagagctgt
    14341 tggtcaggta acagttaaga aagttgatca agatggaaat gctttaaaag gagcggagtt
    14401 cactctaact gacaataaca atgctaaatc tgttaagaca accggagatg atgggttagc
    14461 tacgtttgat ttgcaacaag gcttaacata cagcttagaa gaaacaaaag ttcctgaagg
    14521 ttatcaaggt tcatttaaaa aggatggtat tactgttaat accgatggac aaaaatttag
    14581 ttacactgct acgaacacaa ttaaaaaagg taaagtaaaa gttaaaaagg ttgaccaaga
    14641 tggaaaacca ttagctaatg ctgaatttac cttgacggat gattcatccc aaacacaagc
    14701 agaaaaaaca gatgaaaacg gacttgcaag cttcgatatt gtagcaaaca agacctacag
    14761 ccttgaagag accggagtac caactggtta tacaggcgca ttcaagcaag aaggaattac
    14821 cgttaaagat gatggacaaa cttttgaata tacagctaca aacactttga aaaaaggtgt
    14881 agtaaaagtt cataaagtcg atcaagatgg aaaacctttg gcaaatgcag agttcacttt
    14941 aaccgatgaa aaaggtgcaa cacaagtaga aaaaacagat gaaaatggac ttgcaagctt
    15001 caatattgta gcaaacaaga cgtatagtct agaagaaacc ggagtaccag taggttacac
    15061 aggtgcattt aagcaagagg gtatcagtgt taaagacgat gggcaaactt ttgaatatac
    15121 agctacaaat aatttgaaaa aaggtgtagt aaaagttcat aaagtcgatc aagatggaaa
    15181 acctttagca gatgcagaat tcaccttaac ggatgaaaaa ggtgtaaaag aagttaaaaa
    15241 gacggatgca actggtctgg ctacgttcaa cattattgcg aacaaaactt acaacttaga
    15301 agaaacaaaa gtaccaacag gttacacagg cgcattcaaa caagaaggaa ttaccgtcaa
    15361 agatgatgga caagtctttg aatatacagc taaaaacact ttgaaaaaag gtgtgatcaa
    15421 agttcataaa gtcgatgatc aagggaaagc attagcaaac gcagaattca ctttgacgga
    15481 cgacaaaggt gtaaaagttg tacaaaaaac ggacagaaat ggcttggcaa catttaatat
    15541 tgttgccaac aaaacgtaca aaatcgaaga aacacaaaac cctaatggtt atacagggaa
    15601 atggtctaaa gaaggcatca ccgttgctga cgatggacaa acctttgaat atacagtaat
    15661 taatcacctt gtacctgaaa cagtaagaac aggcggaatc ggtacatcag ccttccttct
    15721 tgtatttcta attgcaggat tgagtatggt gctactgtat tatcgagaac agcacaaagc
    15781 aaaataaaaa agtgttaatg gaaaagaagt aaatatgaaa aaagaaggct gacgtatgaa
    15841 atcaaaaaaa attttttcaa atttaatgct atttggtact ttgctaggag tgggattggg
    15901 cgcaagctca atctcttcta gccttggact aggggggcaa gtagcatatg cacaacaaaa
    15961 ctataattat caaggaactg gaacgattca tatccataaa gtaacaaata ctaatgggga
    16021 tgggaaccct gtctcagata ttgataacaa agggaaagaa atagctttac caaatggtgt
    16081 aaaacctctt gaaggcgttg agttctctta tgtgaaactt actaaagatg aagctaaatc
    16141 cttgggatgg acggatagtg atactacaaa aggtcaacat attgatgatg ctaaagcacg
    16201 attattacta aacaaaaaat acagtgaagt aagtagaatg aagactgatt cacaagggaa
    16261 tatagacata cctatacaag taaatacaga cgatttaaca gataacttgt atttgattgt
    16321 tgaaacagga acagtcaaag gtgtagttga agaagcgtat cctatgattg ttaacgtacc
    16381 tgaactaaac ggatcagatg atcaatactt taattatgat gtacatgtct atcctaaaaa
    16441 ctatttccaa ttaaactcag cagagcttga taaaaaagac gaagatggaa aggctcttgc
    16501 cggaactgtt tttgaattgc gaaaagatga tggatctgat caggttgttg gaacttataa
    16561 aacagacagc acaggtaaat taactgtgga tggtttggaa ttaggtgact attatttcct
    16621 agaaaccgat ttgggaacag atgctaatag aacaacctat ttactagatg gtaaaaaatt
    16681 caaatttagc gttaaacgtg gggagactgc taaagtagtt cttgatgtta ccaactacaa
    16741 acgacctgta attactaagg taattgataa agaaaccgtt gatactggcg aagatgttca
    16801 atatacattg aaaccatcaa ttcctggtaa tattggtaat tacgaaaaat atgttgtcaa
    16861 agatgttcta ggtgcagagt taacttatga ttcatcagaa gtgatcgaag gaatgacttt
    16921 agatactgat tacagtatcg aacgtgacgg acaaacgaca aactatgtct ttactgaatc
    16981 aggtatggat aagcttcaga aacaatttga tgcaggggat cgcttcttta caattttgat
    17041 gaacgctcat actaatagta aagctaaaat ggatacacca cttaagaatg atgtttcaac
    17101 gatctacaac aatggatatg aacccgaaga cgaaacacca gttgatcacg cttatacaat
    17161 cattggtgat caccaattca ttaaaacaga cggagataca agcaaagcat tgagtggagc
    17221 aaaatttgta gtgaagaaca aaattgatcc gaataaagtt acttacctaa aacaagatcc
    17281 aacaacgaaa gctgttactt ggattgcaga taaatctaaa gctactgaat tcgtttccgg
    17341 atcagacggt aagttccaaa ttgaaggttt gcaatatacc tatgatgtag attttgataa
    17401 acaaggtaat ccaacaatca cagataaaaa aatcaatgat tatgctcttg aagaagttgt
    17461 tgctccttca ggatatacac caactagaaa cgatgttcca tttgtggtat cgaatgatga
    17521 tgcttctagc gaagtaacag acatcaagaa ccttaaaaca cctgaaacag ttcgtacagg
    17581 tggaattgga acagtagtat ttctagtggt tgcaggtgtg gcaggaacag ggctatatgt
    17641 tgtctacaaa cgagaacaac acaaagctta atgaaataaa attccctaga gtgtttttgg
    17701 ctctagggtt tctttttaaa aagtgtgtga aaagtaggta tgagcatgaa aaagaagaat
    17761 atcaagttga tcatcttaaa aattgggatg ttgctatgtt taggagtagg tctatatcct
    17821 gtaattactc aggcttatac ctactaccaa actcaacaaa cactaaactc ctataaggaa
    17881 aaatatgatg aagcttctga aaaggagaat gaagaagtct tcaagagata ttcagaagtt
    17941 aataggaaac gaaaagacac agaacaaacc tcagtcacga ttcctgaaga taataacaat
    18001 attccgtcaa atggaaatct tcagaaggat aaattaggtg aattagtggg tgaaattctt
    18061 ataccaaaga ttaatgtgga tttgccaatt tatgatggtg taaacgattt ccaactatca
    18121 gcaggagcag gaatattaac cggaactgat tatccaactg gtgggattgg aaaccattct
    18181 gttatatcag ctcattcagg cattcctaat aaaaaatatt ttacagattt agagttgctc
    18241 aaacttggtg ataaattttt tattgatatt tcagggaaaa aacttgctta tcaagtagat
    18301 cggataaaaa caattctacc aaccgaattt gaggatttgc ttcctgtggc aggaaaagat
    18361 tatgtaacac ttatgacatg tacacctgtt ggagataatt ctcataggtt actcgttaga
    18421 ggggttagca taccttacca aaaaagtatt gttgagaaag aaaagaagaa agaagattta
    18481 aaggtaaagg cgcagatgaa ctggtatcct atcgtagttg taggggtttt attaataata
    18541 gctgtgatag tcattattaa aaaaatagta gcaaagagac gaggaatgaa aaatgaagaa
    18601 taaaatacta ctttttttga ctcttatttg cactttggtt ttaacaattt atcctgcaaa
    18661 tcagctctat catcaattca aaacaaaaga gaatgtggcg gaaatccaag agaaaattga
    18721 gaaaaaacaa actccttcaa aagaggaagt ggcaaaaaaa gtcgaacaaa taaaatcgaa
    18781 cagcggtaac caaggattag acaatactca gaaaacagat aaaggagtag acaatactca
    18841 ggaaacagac ggtggatcat ttgatattcg tgaagctgaa ggaaaggtca atccatacac
    18901 aggagatgtt ttttcaaaaa atcaaacgaa agattttgaa ccatctacct attcaggagc
    18961 ggttggttat gtctatattc caaaaataaa tgtgctagag cttttgtttc taggcgcttc
    19021 acaggagcat ttatatagag gtgtggcaca aatgagtgct actagtctac ctttatcagg
    19081 taaaggtcat caaacagtaa tagcaggtca tcgtggcggt tatacgtcac cacaatttct
    19141 ttatgttgat caattagtag ccggagacca catttatata gattatcttg gtgaaaaagc
    19201 agtttatacg gttttccgaa cagacgttat tagaaattac ggtcagaatt caacactgac
    19261 tgatattcct gatttagata tgttaacact ttatacttgt catccatatc ctcagaacta
    19321 cgatcgttta ttagttagaa gtctaagaga tgaaaaagca aaatacatca cgccacaagt
    19381 aacagttggc aataccgcat acgctgatgc cattgatagt gaatccgttt cagataagcc
    19441 agaaactgtt acatcttcta atgatttatt tgaggatgtt gatcgtgcca acaaggaatt
    19501 agatggaaat aatgatctta atattccgga acaaggtagc gtagagatgc aagacagaac
    19561 gtttgctgaa aaggcaagtt caatatttac aggaattacc aataaacatc agaaaatgct
    19621 tttagtcatt tggactgtat taggagcatt agcaagtcta aaggttctta ttcttttaat
    19681 caaagagttt agaaaagcat aatgaagatt ataaaaattg atagagtaca gtaaaaaggg
    19741 attgatacta ttattgatct cttttttatt ttgaatagag ctagattcac ttttaaataa
    19801 aataaaaact gatcttaatt ccaaagaaaa agtcaagaaa aaaatacaac aaattttttt
    19861 atcagaatat ttatcagaat attattagaa aagataaact caaaagctta atttaatagg
    19921 gttattggtt gtatttagaa ataatgtatg gaaaagctat aaagcaatag ataagatgta
    19981 gaaaatctta tttgtgaatg aaaaacaagt catcgtaaaa tattaatagg aggtgtagta
    20041 atggtggaaa tatcaaaaac agctttagac tcattagcta aactatttag tagcaggttt
    20101 gatggtgcag aatttgttga atatctaagc caaaacgaaa gtggaaaaac tctgatcgaa
    20161 acactgaaaa aagactattt gaatgaaaat gactttccga catggacgaa tattgaagat
    20221 gctacatggg atgtagtgtg tacgatatat tctccttggg taacgattgc tgattttgtt
    20281 ttagataaaa ttggagggga tacataccct gatgccaaat ctcttgctac aagtgacttt
    20341 aaaacgttct atggttatct aatgggtcta ataggcaatg gaatagattt gaaagttacg
    20401 ccaaaacaaa cacctgaaga aattgaaaaa gaaaaaggag aggttcaaat ggcttatgca
    20461 gatgacagag aaaaagtaaa attattggaa ggtaaattat ctgaagaaga acttggaaaa
    20521 atggtgcaag aaatcaatga agacagcaac tattttcctg aattgaaggt atatcaaaac
    20581 gacgaagatt tctttaatga aatgtttagt aatagtccat gggaagcagt gagtaaaaca
    20641 aatttaggag aatggaactt tcaagatgat ctagttgcat ttagcaatga tgggtattta
    20701 cgatcatatt cagaaagtga gtatagagct gaattaaaaa cgtatgaaac ggatattctg
    20761 atgagctatt taaatctatt agagagtggt cagggagagt taagagataa agttgaagaa
    20821 ctattaaaag aagagctata gggattattt caacataaac acaagcagaa aactaccaaa
    20881 caaaacgtac catgcaaaaa gccggttaaa ccggcttttt gctctattat gagaggagat
    20941 caaaccatga gtgcaacaac cgatatggct ttagaagcag taataaaaga taatacagga
    21001 aatgtcgaaa atgtagaaga ctttagcgat attgcaatca atctttcagc atggatttca
    21061 gctgtaggag aagaaaatat cgaatttgag atcaagaata tggatgactt aaaagcaaaa
    21121 tttcctttta tcgcatcgaa actaagtcaa tttgccgagg agaaatcgtg gaatgaatta
    21181 gttagttttt ccgaaaaaat ttatgatatt acgcaagaag atgagtgtgt ttcatgggaa
    21241 atgaataatc aatacaagaa aattgatgct ttcgtcaaat cacgtataga aggaacagct
    21301 tttgagtttg aaggtataga taccgcagat aattggttaa ataaagaatc ctttattaaa
    21361 agtatagcgg atgatcttga aaaacaatgg tctaaggaag actatgcaga gtacctaaaa
    21421 acagaaagag atacagaggt gaaagaaaaa ctcactgagg tatgtttgga tgaacatatt
    21481 gatcccaata tcgtaaaaca agaagactat gaagtggtta ttgataaagt tcctactgat
    21541 tttgaggaag ttgtaaagta tatagttgaa gaagcattat cgcaatactc ttcagatgag
    21601 gaagtggacg aagaaactgt tcgtaccaga gtttggcaac tggtttttga tgacaaagat
    21661 tataaatatt tgagtgatca aaactcggtt aattggagtg tacgattatt aattgatcaa
    21721 aatgatattg aataaaatag agctgaaaag ctctattttt tttgataaaa aaactagcta
    21781 tttttttaaa aataatttgg ttcaaaattt atttttttaa cttgataatt ttttgccatc
    21841 cttttttaaa ttggctatat atcaaggttt atagtgggga taaataaaaa tacatttgac
    21901 aaaatttaca atatagttta ttatatattt atcttattat gataacacaa taaaaggaat
    21961 gatggtatga aaaagaagaa gtttagtcga tcgttgttag gaattttagt tttagttgcg
    22021 gttggtctta gtggcaaggg ttactttcag aataacagca ctcaggaaca tgattttcag
    22081 atcaaaagta gtgttcaggt aggtaataaa gcagacagtg aatcaaaaga atttttaggg
    22141 atacgttatg atccaaacaa atatccaaat aatatcgtaa acattaatga tgggaaatat
    22201 acactgacaa aagaagacca tgaagcatta tcagaaaaag gcagtcaaac cttttgggta
    22261 aattatgcag atttagatca acttggaaga gcaggagatg ttgaagcgtt gatcacctat
    22321 gatgctataa attctcactc atctaaaaat gtggaacgtc catctttcaa aagttctgtc
    22381 catattgcag gagaatacaa ggatggagta tttgatcaag agagagatac ttgggtcggc
    22441 aaagattcaa acaatgaaat cactcagcta gaaggatata aaggttatct ctataacaaa
    22501 agtcattcat tagcatggtc gctaggagga gatatggagg tacacaattt aacacttgga
    22561 acgagagcgc aaaatgttgg gaaaaatgat cagaatggag ggatggctta ctcagaaacg
    22621 attgtacgtg atgcaatcta taaaaataag aatgaaaaag tattgtataa agttgatcca
    22681 atttataaag ggaatgaatt ggttcctcgt ggttctcatg tcgaagcttt ctcagttaac
    22741 gataatggaa aaacggtaaa ccttaatgtt tatgtattta acaatcaaaa aggaatcaac
    22801 atcaactatc aaaatggtac ttgggtaaga gattagaaat tgagaaggtg aagaattgga
    22861 aacaataagc atcaatggat tagataaatc agttgcaaat cgaataaaaa aaggaaatgg
    22921 ctactatctt ctgaagaagg gtaaatattc agttgctaga gagttagaaa caatgagtat
    22981 cgtgtatgta gctaagaatc attttgacgt agaacaggct atttttgatc cagattctga
    23041 aatagggaaa tgtgttgaat tatcagagaa aactgttagt acaaaagggg cgatgatcat
    23101 tgagagagaa atgttagacg aaatcagagt aatcattcct aaagatacgc agatgtgtga
    23161 ggacagcata agtgacgatg aaccaaaatc agaaagaaaa taaggagata ttcagatgag
    23221 cgaaaaatcc attgaattaa aggaatataa gcgaagcgat ggagaaaaat attatcctgt
    23281 agccagtttt tctaaatcag tatatctggt tggacgtgca ttggtagttg gtgcaattgg
    23341 catatatatt ttgcattggg taacaaaggt tgtacaactg cttgtagata ccttagtaag
    23401 tattcttgtc tcgttaaatg atgttaacgg aacagatgat ttaggattaa aagaaatgtt
    23461 taaagagtta ctcaatgcgt tttttcccaa ttatttaagt tattcatata atgtacaaag
    23521 cttttttgca aagctacact tggtagacat ctctatttta gtattagaag caggaacggt
    23581 attctttgtt ggatatttgg ttactttgat gttcaggcat cactatggag aacaaagacc
    23641 attttttgat gatatggaaa gtagaaggtt gaaaagagaa gctctagaag ctatggatgt
    23701 gaagtatgat ccaaacaaga ttataggaga tgaaacgaac aacaaaaaaa gaaaacgaaa
    23761 caaaacgcaa ttagaatccg atgtgctatc aacgcttaaa aatatcaaaa tttctgttca
    23821 tacaagacgt gatctaagtg tcaatgaaga tattcgagaa tatatcgtaa aaatcccgca
    23881 accaaaaggg aagaaagtta gagaaacagt tttacgacaa attcaagatt tagggtcgat
    23941 cctgacaaga gcaacaagcg gacgagccaa atttggtgaa aaagaagatg aagtttcagg
    24001 cgattatttc attttccgag catcagtaaa tattgaaaga gaacgtaagg aaagagtaag
    24061 taccaaaaac aaagaaacgg aaagagcaac atcttcaatc gcagtttcaa attcacaatt
    24121 ttcttttcca atcaatctct ttgccgataa ttccaaaaat atccaaaacc aaaccgaaaa
    24181 agctagaaaa tatgccgaaa ctcaaaagca atctttagaa ctttatctat cttctgctga
    24241 cgtacctgca agtctaacag atatgcacgt tgggaataca tctattgaat atttgtttac
    24301 tttatctcgt accagaaaaa ttcaactaag ctcaatgaaa gaagaaattg aaacttactt
    24361 gcatatatca ggtgtaatcg tgcggatcaa cgcagggcgt attcaaatta ctctaccatt
    24421 aggagaaaat gatcgtattc caattgatgt accatcaatg atcaatagcg tgttttaaaa
    24481 gacatcacaa atagaaacag aaaatacaaa ctaccagttc acaaacagaa aagtaccgag
    24541 aaaagtgagg aatagaaatg gctatgaaat tattagagaa agtcaaaaag agagccgaaa
    24601 aagctgaatc tattttgcta gataaagaac ctaatataaa gcttaatgga aaattcagtg
    24661 tcaatatgtt gaacacaaag gctaaagacc cgacagatgc tatttttgga attaagcctg
    24721 atggcacaat tgctcatttt tgcatcagcg aaatgccaca catgttggtt gcaggaacga
    24781 caggatcagg gaaatcagta ttactgaatg aaattcttgt tacagcaatg tgtcactcaa
    24841 cgcctgatga attaaaaata ggaattgtag acccaaaaag ggttgaattt ggaagataca
    24901 aaaaattgcc gttcatgttg gctgatccaa ttacagatat ggatgaagct tatgattttt
    24961 ttgaatatct tgtgatcttg atgcatgaac gttacaaact gatggaaaaa gcagggttac
    25021 aaaacatcac gctatataac gaatatgccg aaaaaaatgg attagataga tttccctatg
    25081 tgatcttatt agttgatgaa tattcacagt tggttggaac acataaagaa gtagaaggtt
    25141 tgattgttca attaggacaa atggcaaggg cagcaggtat tcatgttatc ttagctacac
    25201 aatctcctcg atcaacagtt gtcactggta ttatcaaagc caattttccg tcacgtgttt
    25261 gccttatggt agcaagtgat ttagaaagta ggatcgtatt agatgaaggc ggtggagaaa
    25321 gcctaagtcc aaagggagat atgattatta agctcgttaa tggaagtatg gttagagcgc
    25381 agggagctta catcagtaac aaagaaattg aaacaatttt caatcacctt agaaatacta
    25441 tgccagaacc agaatatgtt gattaccgag caatcgtagc tgaatctcgt ggtgagggtg
    25501 gagaagacga ggattttggt ggaattggta aagctcctgt aagaaaatct gctaaaagaa
    25561 atttgaacac acctttagca aattcatcat ctaaagagcc atcaattgaa aagaagagta
    25621 cgtttgctct aaaacttgaa aaaaacaaag aagaaaaaga aaaacaagga gctaaaacac
    25681 tagaaaaact tcaagccgaa aaattagaaa aagttttaaa agcattagat aaagctgatt
    25741 ctgaaccgca aacctcaaac aacacgaata atgaacagcc gatttcatct aaaactaaac
    25801 cgaaaactac tgatccgaaa gtttcagtaa acgtgaccat cacaaccgat aagtctgaga
    25861 agaaacaagt aaacggaacg acttcaccta tcatgcaaaa aatgttgaaa gccaaaaaac
    25921 aggtgcataa atgatcattg tcagttcttt tttgaggtgg cttacgccat ataaatatac
    25981 agatgaagta gttgtgaaac gaacgaagaa aagagataaa aaagcggtac taactcctaa
    26041 acagatgctt gatcatggca ttttaggggt gaaggtttta gaagtgaata aaccgtttga
    26101 atcaaatagt gcaagagaaa agttgcttgg agctgaagcc gatgtcgcat atttttacca
    26161 tgacaaaagc aagcatcaaa tagctattaa attcaatcca aaatcggcat ggcggtatca
    26221 agcaagagat gacttgaaac gtaggaaact tagaccaatg ctctatccag aaggaaaaga
    26281 agcatttgat agaacccaca tcgttcccat tggttttcat ggtacagaat cagataaccg
    26341 cctattagta ggttggaaca gcaatcaaaa tagagttcag atcaataaat ttgagaagaa
    26401 agtaaggaaa ttcaacgatc gaaaaacgat cctatggttt gcagatattt gtaaacagcc
    26461 ggatggaaca gcaaagtggg aagcggtgat atttgacgaa aatggtaaaa aagtaatgtc
    26521 tgaaacatgg catgataaag ctaaatttgt ttggcagtag cagtagaggt gaaaaagatt
    26581 gctagaactt tttgaagcaa atagaacagg ctttaatcaa aagttagaag tgggagaaac
    26641 tatagaatat aaagataaaa actatattat tatcggaatc tataacacga gaattaacag
    26701 aataggtgat ccacgtataa ccagtgactt ggtttgtcaa tctgttaact attcacctga
    26761 tctgacaagc aggtataaga aatacgtgtt attagaatat agacacaaga ttactgatga
    26821 aattggtgta aacaacacac gaaacatatt taagatcgga acagtgattc cttatagtaa
    26881 caacgaagaa aaaatatttt atcagattta cggaatagaa aagtttgaat atgaacatgt
    26941 cgatctgcta gtgacgtacc aaatgcgttt aatagaacct tggtcacagg cagaaattga
    27001 taaagcagta aaattgaacc gattatcaaa atttaacgta ttaggaaatg ccttttaaaa
    27061 atcgcaatac acaaacagaa aaataccaaa aaattagcaa tgaacgtagg taagaacatg
    27121 acaataaata ataataaccc taaaaaatat aatgctcttg tccaatgcta tatcgaaaac
    27181 aaaattacaa tgcaagaagt cgctgatgaa ttaggcaaat caaagagcct agtatcacac
    27241 gattttaagc acaatgtttt tccgaacccg attgagctga ggaaaaaatt tcaaaagaga
    27301 gtttatcaag aaataggtaa gtggattgat gaagggaatg caattgaccc tttaattgaa
    27361 aaatatcctt ttttaaaaag caaaaataaa attcaaaggt taattcgtca aggcgatatt
    27421 caagctaaag aacctgtttt gacaaccaat gatttggcta aaataaatct tcataaacaa
    27481 attattgaag actttgaaaa aaatctttca acaactgaaa ttttaaaaaa atacaatatt
    27541 ccgagaggaa agctaacatc gactcttttt agagaatatg gagaatcggc agttactaaa
    27601 gcaaaggaag tagcgaatga gagaaaaaaa caaaaatagt agtcgatacc tagtagcgac
    27661 tgtagttttt gtttgtgtat tgattttcct gtttgatttt ttgcagattg gaaaaagcat
    27721 aattggaaat acattgagtg caaaggaaat ggttgcttta ggtggtttgt atgaaggaca
    27781 cttgttcccg aatatattca tttctatcgt ttctcatatt aacttgtacc atttaatttc
    27841 taatttattg ttcttagtag ttcttgaaga agcactgaat gatcagtatg agtggttaac
    27901 attattgatc gttttaatca tatcaggatt tctaggaagc ttagttcctt ttctgctaca
    27961 cagcaacatc gtaagtatgg ggcttagtgg aggtattttt ggatggattg ctttatttgc
    28021 gattgcatac atccgttttg atagcaagaa acatttatta ctaagtgttt ttgtattatt
    28081 gataatcggc attttactgg tatctacttt tgaatcaggc gagacaaacg tcttgtcaca
    28141 tgtaatcgga tttgtctgtg ggatgatctt gggatttatt ctaaagaacg tttcattgat
    28201 caaacaaaag aattaaaagg gagtgctaaa aatgccaaaa aaaaaagaaa agaaaattcc
    28261 agtgtttttg tctgtctcag aaatgttatc acttcaaata ctgttaagaa aaaagattga
    28321 aacaatagac gaagaagtta gaaataaaag ggctatgtac gaacaacgtg aagcgctaag
    28381 agaacgaatc cactattttg atgatataca cactaaaatc tatgaagcac gtacaaatca
    28441 cgaagacgaa agcaaattag gattagctca acaacgaata tttgaatggc tgaatacgaa
    28501 tactgagcaa gaaccgttaa gagcaattag taatcttttt gaatttggaa cgaagcattg
    28561 gtttaacgct gtagaaaagg atgctgactt acaagaagcg ttcgatacac taagcgagga
    28621 tagcaaatcc atgcgagact tattgatcac ttatctaaat gagcatgaat ttgaacagac
    28681 gaaagttaaa aagaaaaata aaaaatagaa agttgtgatg tatcatgctg tttaccttat
    28741 taggttattt atgtttagtt cttgcttttt ttggagcaat tgctatattt gtcggtcagt
    28801 ttcaagaaaa taatccgata atgcgtaaca taggcattgg aattgttatc atatccttga
    28861 ttgtatttgg tgccacaaag atttttggga gtaatgatca gcagtttggt atttctggag
    28921 ttgataatag ttatgctcca gattcattgg gaagagtagt gatagatgga acgtttgaga
    28981 atgtaacgag ctacaaaaat gtttcattga cagtgaaaga agatgacaag aaagcatcag
    29041 atgacgttct cgctacgatc aagccgtctc aatcacagtt tgaagtgttt tatacagtca
    29101 aagagaataa ccctgtacgc aagctaacat tagtttttaa cgttgacgga gaagaatact
    29161 ataaagatgt aaaaatcaat aaagcatcaa cgaaaaagat tgtcgaatca gcgaaaaaag
    29221 aagtagctac agaagaaagt acaactgaat caagttctac tgtcgaagtt gaactaccag
    29281 ataaaattga ttttgatcct gaaaaagtca aatcgtttga atctgtttct ggttctatgg
    29341 cagatcggtt taacactgaa gttttcaaag ctatcaaaac tgttaaattg gatactgaaa
    29401 agcaaaggat taatgttacc ttattcaatg cagttgaatc tatggatcag gataaaaaag
    29461 ttacggttgc aagagaacta gggaaaagag taattggatt gtatcgaggt acggcaaaac
    29521 cagattcaga cggttcagat ttgccagttc acgtagttta tgaagacggt aaagtttttg
    29581 gagactctac agcaaatgaa gcactaacga ttaaattagc taaatagccg gaaaattata
    29641 ataaacaagt ggtgtcacac caccatcgca taggtgtgac accttggaga tgattactga
    29701 tggtagtaaa taaagaaaaa gtaagaaagt atgttacttt aaaaatagct tctgttgaat
    29761 atttagaaaa gcgagctaaa gaagaagaaa aaacaagagg taagaaggta acagtttcag
    29821 agttgttaga agaattaatt gagagtgacc aaacaataag acgtgcattt accaataggt
    29881 ctgttaaaag ttagggtgat actatcttta atttcaacca aatttctaat atatgatgaa
    29941 tagtttcctt gtacaaaata tctaattaag agataaaagt taggaggatt cattcaaaat
    30001 gattatccaa aagtttgact ggaaaatgaa attaggaatt agtcttgtaa tattgggatt
    30061 tattattttt ttggggataa aagcacaaaa aaatcaatct acttttgaaa ctatgtcttc
    30121 tcaaattatt gaggaatgtt ccaaaattta ttattgtgag aacaaaactg aagaatctaa
    30181 tggcttttat tatatcgatc aaccacatga tgttgttata aaaatggagt cacatgaagt
    30241 aaacgaatgg attcaaagaa gtactaatgt atcaaaaaag ttagaaagat attctcaaac
    30301 gtttttaagt aaagtcattt tttcaaattg caaaaatgaa gaaacgtctc aaagagtagc
    30361 tagttcttta gaaaatttaa aattttctgt ttcaaaaaaa gagaaatttg tagatttacg
    30421 aaaataaact atcgaaagta gtatgattat gatcttaaaa tatcatctta agagaagaat
    30481 tacgattgtt tataggattt acaaaatgaa taagtttgaa gagtgatttg gtcgcagact
    30541 agatcactct ttttgtgtaa aaaaacttta cacttatttt tgagtgtgtt aagatttttt
    30601 atatagagtg acggtttctt gtcagagtgg atgataacca tcaatattgg aggaataata
    30661 agtggaaaac aataatgata ttagatattt tagcattggg gaaagagtta caacagtaag
    30721 tgaattatca aaggattata gagataaaga aggaacgatt atttctctta aaaagaatcg
    30781 aagaggtgtt tggcaatatg ttgttgattt aggggatggc gttacagttg agtgtaaaga
    30841 tagtcaactg atcagaaatg atggcagtca ttccattgaa aaagcaattc ttgtattccc
    30901 aaaagtatca agcaagatta tgaatgactg gttgcttgga aaatttggtg agtacaataa
    30961 aaaagaaatc gcagaagtga tcggcaaatc ttatccaaca gtattagggt atctaaaaaa
    31021 gataacgaac aaagaagcga aagttgagga tgttttaaca gtaagagaac ttcagtcatt
    31081 aatcgaatat cggaataaac aaataacaaa tcctgagtac cttaaaaaag aagatggtct
    31141 attgaaacaa ttggaacagg taaataaaat ccggaaaatg caaaaggtag ctcctttaag
    31201 cctagataac tttaaaaaaa tctatttgaa agagatcgaa aaagactaat gtggagtgtc
    31261 actttaccgt ttcaacctgt atctgcacca agaccaagtg caagaaggaa tgttgctgat
    31321 gcaactaatg acgtagcaga gaaaaggatt tcaacctatt atgataaaaa gtatctcgat
    31381 tatttgaaaa aaataaaaag ctttatcgaa gatcaaggtt tgttagatga tgaattttac
    31441 tcgatcgtaa atgatccgat gggtgcaatt atggaagtgg acttttacta tcagatacca
    31501 aaaagctata aaaaagtcta taacattatg aaaacgacag cgccagattt agataattta
    31561 gtaaaaagtg ctatggatgg tattttcaac atctcagcta tacgagatag ccatatcaca
    31621 gggttaattg cttttaaata caatgtggca aacgaaggaa aaacagttgt gaggatcaag
    31681 cgatattcag aatttgagtg ggatacttct gaaggattga acggagatat ttgggaagcc
    31741 acatttgatt tcaaaccagt agcaacgcca agaccaaagt ctaaatttag aggaaatggg
    31801 attatcactt actatccaaa agagtataac gactatttgg aaaatatcaa gaatacgctt
    31861 aaagagaaag atttggtaaa tgatcaactg aaaaaggtaa tgaatgctcc tatgggggtt
    31921 atagctcaga tagattattt ttaccaagtg agaaaagata agaaaaagct tgattcactg
    31981 atgcgtactt cacagccgga tattgataac ttagtcaaag gaacattgga cggaattttt
    32041 aacaaggaaa ttggcatcaa agatagcagg gtggtaggtt taatagcttt taaatacaat
    32101 acttttgaaa agtcgaaaac aaacgtgcga ctacaggaga tgggctgatg aataataacg
    32161 aaaaaattct tcatgtactt gatagctttg aaacaattca agaggaacta aagaaatatc
    32221 gagatgtttt ggaacaaaga tatgattttg tcaatcagca aaagagtaat cacatggatt
    32281 tcattctcaa tatgaatgac ttgaagaaga aattagttga acgaaaagaa caagaaaagt
    32341 tgatcaaagc ttactttgaa ctcggtgaaa aagaagttaa aaaggcaatg gaactgaatg
    32401 aagatcgcag ggttttagat cagctgttag aacaattatt ggtgatgttt caaaaaggac
    32461 gaatagatga ggatttaatt gaagaagggt tgagaaagta tcctgcaaac tcaggtatcg
    32521 gaatagtgct gaaagcgatt gatgaagaag aaattgaaga tttcataccg gcagaggatt
    32581 ttgaatcggc aatggaatat ataaaatatt acagccaagg gatcactgct tttagagagt
    32641 ttgatcctga agatgttatt catgacttga acaacctaaa agaatggtgt gaaggttatg
    32701 aagttgatga ttccggtttg gactacttga tcagtattat ggaaatcgaa gaagagatgc
    32761 cggataagcc tgatccaaca gatattttag aattaatcca tgatgctaga aatccaattg
    32821 catacatttc tagaggatat actgtattgg aatactataa gccttatata tcagcgatga
    32881 accatttaag acgtgtatta agggaaaaac gtgaataccg ctcagtgtta aatgcatcga
    32941 atagattaga aaaagctgtt tcagagctag atgcttatta tcgtgaacat tacctgcaag
    33001 caggtggtat gccaagaaat acgaaagcaa atatcagtag gtatataaaa gaagctgagt
    33061 aggcggagtt agcccataaa atggcaaaag gtaacctgaa agaggcgttt tgaatgaaaa
    33121 gggatgtttt tgaattgtac gtattagaac gtctagaaga cggcatgatg gcacaagaga
    33181 taattgatga aattgatgat atgatcagca gatttggtca attggaattg aatgatggaa
    33241 acaaaaattc ggtagtgcta caacaatttt ggttaaataa tttttcgtat aaggatttta
    33301 tcaaagaaaa ggcaaataga ttgtactgac taaattcacg aggatcagtg atgagagctt
    33361 attgctaaga gcagttaggc tcttttttgt ttgtgttaaa ttattttatt ttacaataca
    33421 catttataca caaaaaatgt tgacggatag tacacaaatg tggtaggata tatttgtaag
    33481 caagttagat taacaagtta aaggaaggaa gagcagttat gaaaaaagaa gaactaatag
    33541 atatgtttca aattgttgaa cgagcaaata atatgggaat tatgtttttt gatcgtattt
    33601 ctttaaagat ggatttaagc gttgcccacc aagaatttaa tctacgatta aaagctttat
    33661 tgatttctga tgatgttaat ttcgctcatg atgttgtagg tatacagaac catatagata
    33721 gagaaaacaa acgtatgggt gatggattct taccaagata ttcaagttta tgatttggcg
    33781 aaagattcaa gagtatgtgt cattattgat aggtactctt gaattggaaa ggaggaatga
    33841 atttaagaca acaacgatta tgatcttaaa acaatcgttt atgtaattgg caatcttacg
    33901 aataattttc atgcaataat gacaataaaa aatgaaaagg ggtaaaatca aatgaaaaaa
    33961 tttttagtag gaattctatt cacaatgggg ctagtgctat tatcaccttc agtttcagca
    34021 gaaagctcta tttccatgta tcgtatgtat aatcccaata gcggagagca tctatacact
    34081 agaagcgttg gagaaagaga caatctgaaa cgttatggat ggcattacga aggtgtcgcg
    34141 tggcaagctc caacaagtgg ttccccagtt tatcgcttgt acaacagata taacggtgaa
    34201 catttttaca ccgttgatgc cggagaaaga gataagattt cgcacacagg atggacgtac
    34261 gaaggtgttg cgttctattc ctacaacgga gcaaatagag ttcctgtgac acgcttgtac
    34321 aacaaatacg tgaattggca ccattacacg ttagatcaaa atgaaaagaa cgtaattagt
    34381 aaacaaggtt ggaagattga aggtacgggt tggtatgcaa tgccaaacag cgcaccaaat
    34441 cctacacctg cacctgctcc aagtaatgaa aaagtgttaa atgttcctgt catttggcaa
    34501 ggtaatacga tgttgtgtga aggtgcttcc ctgttagagg gattgcacta caaaggagta
    34561 gcgacaaatc aaaacttaac cagttttgta aactatatgc caagagctaa tgacaataat
    34621 ccatatcatg gctattctgg cgaatggcgc tacaacgtaa atggtacgta tcaaggcatg
    34681 atgcctgatc cagtagttaa atgggcaaaa aatgttggtg gtaatgccgt taatatcact
    34741 ggttcaggaa cgaatgggat caagaatgaa attcgcaatg gtaatccggt tgtaacttgg
    34801 attacttata actatgcttc acctcaattc aaacaaatgc cttggggacg tgcagtctgg
    34861 aatggacatg tggttctcgt ggatggcttc aaaaatggtg cataccatgt tgttgaccct
    34921 gtatttggcg caaaatggat caatgcaagc acgtatgaga gagcatttaa tgtgactgga
    34981 atggcggttg ctgtacgtta attgatagaa ttaataaata aatgaaataa gagcttaatt
    35041 actgaaatgg taaatgggct cttatttttt agaaaaaaac tgttttttgt aatacgaatt
    35101 tataacaaaa aacgttgaat gtagtacaca aaagtggtat attaaaaccg tagagattat
    35161 gcgaagaggt gattttgatg aaccaaagaa gaagaaaaag gctagaagaa ataagcgagc
    35221 gactatccga gttaaaggat gagttagaag taattcaaga agaagaggaa gagtacaaag
    35281 aaaatatacc tgaaaatcta cagggatcag aaagatatga aaaagcagaa gaatccgttg
    35341 atcaacttga agaagctgtt gatggtttat ccgacatatt agaattgatt gaagatatag
    35401 ctagctagag atgattaatg aagatttcag gagtgagcaa aaaatgattg atatttcact
    35461 gatagagaag ctactttttt caaataagta cacagccaaa gagttggcta caagatggca
    35521 aatatccgaa tcagctatta aagcctatcg tcaaaataag ggaagcgtag gatggcgtga
    35581 ttggaaagga atcagcattg gaaaagcaga agacataatg aaacgtgaag gatttcaagc
    35641 tgacaatgat tctaaaaaag ttgaatagtt taatagtcgc caagaaaagt accaggacga
    35701 ggggaaaggt agattacaaa ggaaaacact tttaaataaa cttcgcaaaa gcatcaggtt
    35761 atgagtggct agggctatac gaatgtgatg aaaacaaagg gctttaatgt caaagaaaac
    35821 gcaatagggg gggaacaaaa tttttaaaaa agatatttat gtttggttta atggaaaaaa
    35881 atatatgact actaacaaaa ggtggtcaga gctagtacaa aatacagtta aaaaagtcat
    35941 gccagatgga tatcatttca actatataca atgctcacaa acaactgaaa gtatttattt
    36001 ttcaatatta aatgagaata gactctatta tttcagggta agttcacacc ataggaaaaa
    36061 gagagaagat aatgttacga catttttatc gggaaaatac aatggcttca aagagttaag
    36121 tgaagccatt ggtgagtatc taaaaagtaa tagatttgtc agaatttctg acaaacatat
    36181 attgatcttg ttactttatt tgaataatag cttcaaattg ttttggagag acgacacttt
    36241 ctactatatt gagaacggca tggatattta ttctggtatc aacggcatat taaaacatta
    36301 tattaaggcg ttacttcgat atggggttct ttatcaagaa gatttaagta accaaattaa
    36361 gattagtaat tctggaaagt cattactggc aaaatataaa aaattgcttt ctcctatcta
    36421 caaaggaata gatatggaag ctgtaaattc tgaaacttta tttaatttag ttgttgatga
    36481 aaagtttgcg aatgaatttt ttaaaagtaa aaaaattgaa agactaaata acctgtttcc
    36541 tgaaaatgca ataaaagctt atgtagataa taataaatca cgattataca actggtttat
    36601 tcccaaacgg ttgcgaggaa aaatcaaaat tggcgatcaa gtgaaagtaa ttaatctaaa
    36661 tcatactaag atcgttaccg taacggagtt gtatatagct gaaaaagaag atattacaaa
    36721 aatgaaacca gttattaaaa tactagataa caaagagtga gtggaatata tggtagcaaa
    36781 aaagaatact ctaaaagaag cttctgaaaa atatcaagat atgaaaagac gaggattaat
    36841 accaaaggac aaaaaaggta tatggagaga atttgttaag gaagtcagta gtgatcgaag
    36901 tggcggtgac tttgttggac gatatacagc aaaaggttat agcaggcgct taaggatcac
    36961 tgttgaatat ggaacgaatg aatcagctac agagctgaag caaaggtact tagatacacg
    37021 aagagaagct tcaaaaaaat atgatgattt caagagcaga ggtctaattc ctaagaagaa
    37081 aaaggggatt tggcgtgagt ttttcaaaga agaagatagc aatcggaata agggagactt
    37141 cattggacga tacacagcaa aaggctacac taagaattta gggatcaagg ttgaatacgg
    37201 aactggtgaa agttcatggg aattgagaaa tcgctattta aatacattgt tagagaaata
    37261 taaagcagaa ttattggagt tgttcgatgt taatgatttt atcgagatgg ctgaaaagta
    37321 tggtcatgaa gaaggtttta tgatcaatga cattatggtt attgataatt ttacggattt
    37381 attagcactt gcgaaaaaag caagggattt aagagaaacg cagatgtatc tattcagaaa
    37441 taaaaacgga agaaaccgga gaactgaatc gagtgagatt gaagattact atgatgcctt
    37501 tacacacgat ctgggagcct ttgtagacga gataagtggt gtaatgaaca aatatagggt
    37561 ctagaataaa aaagccggag atgagcagat aaatgggaaa caatagcata tacacacctg
    37621 aaatgcagaa taaaattaaa agacttgtcg gattagggtg gatgacaaga ggaaaattag
    37681 attcttttag tacgatcaaa tatcttttga tggacattta ccgtcagaac aagcaaacag
    37741 gaatacattt tttgggctat gataagccgg aagatgcacc tttctttgtt ccaagttcag
    37801 cctatagagc cagtacctcc ggtagcatgt ataacttgga tttgtttgag gaaggagcaa
    37861 agatgatcga atatgatttc aacgaagcct attcgaggat catgagaaat tatcctttgc
    37921 cgtcaaacac ttatttgaaa gcaaaatata atacagatca agctattttg agagcgataa
    37981 atagaccaaa agtaagaccg tatgaaaatt taaaaacgta tatgttcttt ttggttaatt
    38041 ttgaaggaac agccaaagaa ggaacatatg ctaatccaaa atcacagtcg atgtttgcgg
    38101 attatggcga aaatcccaaa acagctaatg agcctatttg gttatcggag attgagctaa
    38161 cacttatcct tgattattat gatattaaag agatggaagt cctagatagc cacacatttt
    38221 tgacgaaaaa aggattactc aaagaatatt ttgaaagaat cgagccatta aaaactgatc
    38281 ctgatacgtt gaagctttat aaaaatctta gaacgaaagt atttggtgct atcggacaaa
    38341 aatcattaag agaaggagaa acaagcacct tcgataaata cagtatgtat aatcgagcgt
    38401 attcctccat ggtagcaggc gtatttagag atcgtatggt tagatatgaa cagaaatacg
    38461 ttcatagcgc atatgggcta ttaaaaattc gtactgatgg attgtatttc agatccgaag
    38521 taccagagtt tgaagagctg tataaacttg gagtagttaa gaaaaaagtt catacaatat
    38581 ccaaagaaga agtcgaaaaa gctttagaat ttaaaaacaa aggatttgaa aataagggct
    38641 cctttgggaa atctaaagat gaagttaagg gtgttgtgat agacgatgat ttcgagtttg
    38701 attttgacta aatactcttt ggaaaagatg gtaggaggta aatatgaaga agaaaattgt
    38761 ggaaggaaat gtaggtgtta ttattctaaa agattcaact gactatgcta aatcaatggc
    38821 actaaaagac gaaaaagttg cacgcatgga taaagattta gttgaatttc tgatggatga
    38881 tacagaaaga ttaaaagggg atcagttttt gagtttaatg aaagacaaag gcttaaacac
    38941 atatggttta tttgattata agaaattagc tatagagtgg gtaaaagaag gagaaatatt
    39001 ttttattgaa gaatgggaag gtactgagaa cttagttctt catcagtatc ttttgaaagc
    39061 ttaatctaag taaaacagtt ctgactagtt atgcttgtca gaatttcaaa ggaagttttc
    39121 cataaccatc atcattcaaa aataaaggat ttgaaaataa gggttccttt gggaaatcta
    39181 aagatgaagt taagggtgtt gtgatagacg atgatttcaa gtttgatttt gactagattt
    39241 ttttgcaatg tttaagagta aacaaggtaa gaactaagat catgaagatt gagagtaaag
    39301 aagatagagg acgaatgtat gagaacacac tatatttatt tagaagataa aattgtagat
    39361 gaaattccgg caaaaagtcg agaacaagct agaaaattat ttattgaaaa atacaaagag
    39421 atcaatggag atagtgaagt tccaaaagtg gtcgtaacag ttagaaaaag gcaaacaaaa
    39481 aagagaagta cgtttcatgt cagccctgat atatataatc ttatagagcg acatagaagt
    39541 gaagataata tttctatctc tcaatatatt gctacagtta ttgatgaatt gatttcagga
    39601 aagtatgact atgcttttga ttttgagggt gaaaaggatt acaaaagtgt tgctgtcttt
    39661 caatctgatt ttgagaagct aaaaaactat gctggcagga gaaatgttaa ttttgcaaga
    39721 ctactagaag agttctactc cgaagagttg ggagtaggaa atgaatacaa gagagaaact
    39781 tatgcgaaat tgtctcataa gttatcccaa gcgcttaaag aagtgacaga agttaaagcg
    39841 gaactagatg ctaaacttga agaagtgaaa aaatatcaaa cagagaggaa gaacaacatg
    39901 aagaatcaag tggaatttga cgaacagaaa gaatttgaaa aagcaagaaa acgctataac
    39961 aaaattatga atgaacttag ctatgaacac ttaacagttc atgaataatt gagtgagggt
    40021 acagaaaaat atacgatcgc agatatggtt aatgaagtgg aaactctttt agcttctttc
    40081 tttgaaaatg gtcacgtaaa cgatgaatgg cgaaaagaag aacctaaaag ggttaacgct
    40141 tatattgctc gattaaaaag gtttattgat aaatataagg agaatagtga caaattttct
    40201 aaagataaat aaggagagag tgtgattaca tttgccagaa atcgaaggaa tattttcgcc
    40261 aaaaataggt aaaaaacagt tagatttcag cagttttccg agcgtaaata gagattataa
    40321 tctaggtgat gaggtacaga tactaaatat tgatttcaaa gaactagact tcgctgttgc
    40381 tgtttccaac aaacccaagg cattggaacg agtgaaaaaa atataccaag agaatgatca
    40441 aaatatcaaa aatatgatca aaaagtatag acgagataat tcaaaagttg aagacgtttt
    40501 attcaacggt acggcaaaac atgatttgaa tgtttatatg gcattaggag tggctttgta
    40561 ctgtaattca attaatcaga cagatatttt ggttaatatc gtgaggaata gctatcaaaa
    40621 aatattaaaa ataagtagcg aaacaaaccc ttattatgca atgaaatatc ttggggataa
    40681 tttattaaat gataaaaaat tgatgcacaa cactgaagag ctgttatcta atttggctgc
    40741 gattaacttt ctattcaaaa caggttctga agattatttt atagataacg tactaaaagg
    40801 tgcttacgga acagcatttt atccgcttta caatatgaaa cagcataaag ccgaacaaaa
    40861 ggataacaaa cttgaaatta aaaagatgaa agaactatta ggtataggat ttgtaaaacc
    40921 caattcaaaa gtcactattg cttcgttagc aactactaac aatgatggga aaatagtaaa
    40981 tgggtttgac gattcagata tagatatatt gtcaaaatca tatttttctt tgtatggaga
    41041 ttttgcagat ttgatgggta ttgatagtta cttagtcaat aattttgaga tcaagggtag
    41101 cgagctgaat gatattgtgt atttcgtttt aatctctcta tatgcaattg atcagaatac
    41161 cgagtaccga caaattgact atttgctagt tttttatagt ttgttatatc agttcgtttt
    41221 aactcggttg tttaaaaatg ctaaggaaga attgatcctg aattctgatt atgaaatagc
    41281 aaaacaaatt gcaagtatta gaaaagaaaa caaatctgaa gtaatcaaat taagaaaaga
    41341 attaagtatt gcgagtaaag aaaacggtcg gttagaatct ttagtcaaat cggcaaaaaa
    41401 agaagaggaa cttttgaagc aagagattaa agaattaaaa ggaatcagaa aagaacttga
    41461 acagctgagg tttgaaaata aagtattaaa gcaattgcag gagcaagaac atagacaatc
    41521 attaaatggt aaaactgaag agccttcact gtctgaaatg atagcttatc taaaacataa
    41581 aaacattctt ttagtgggag gacatgtaaa ttttcaaaat aaattaactg aactattacc
    41641 aaatattaaa atgtttgatg taagcgaaaa taatggaaag atacttaaaa atttgaatac
    41701 gtgtgatgcg atttttctat atacagatta cttgaatcat ggtctatatt atcgtgttat
    41761 gagtgaaatt tctaataaag ataagatatt ttatttggat aatcatgtga atatgaactt
    41821 aacaattaaa cgtatttacg atcttttatc acttatataa agagagaagt gataaatagg
    41881 acacaaaatt tggaaagtag gtaagtcact atgagagaac taactttatc ttctaaggaa
    41941 ttagaaaaga aaactgcttt attaaacgaa atatttagtg ctgattgtat aatccaaagt
    42001 ttaaaagaaa gttcattttc aaaactaaca gatcaggaaa aaatttctta tatgctaagt
    42061 ttagataatc taggaactaa atatgatgaa tcagcaagta gcgataagct tttgattgaa
    42121 ttagtatcaa gtcttagtag ttttggaaaa atgaaaagat ttaatgtgat attagaaatt
    42181 tttgcttggt taatcccttc atttatttgg tttttttata ttttcatagt tgaatctgag
    42241 ttaatagcca aggtctctct tggactgtta attccgatat aaataggttc agcaatttac
    42301 tttaataact tgtatacacg tattcaaata aaaaagcagt tattacatga aggattaagt
    42361 ggtaatatgt taaaaagaat tgcatgggat aaagataata aaacagatat tgagcttaaa
    42421 gaatataaat acaattattt ctcgagatgt atagatagaa agttactgtt taaaaaagaa
    42481 ggattagaat ttagttttga tgaagatgaa tttgaaaaat tagtgatgga ctggcgtaag
    42541 aatttactgg atacggtaca attctaaaag caattgaggt cgaaattccg atacttgaat
    42601 catgatctat attatcgtgt tatgagtgaa atttctaata aagataagat atttcatttg
    42661 gacagtcatg tgaatatgag cttaacaatt aaacgtattt atgatctttt atatgtttat
    42721 aaacattaat gaggtgctaa aattgacaac aataactatg acaaaaaaaa tagaagtata
    42781 tgttgatgga tcacaagaag aaagggattc cttttttgat tttttttgga aaaatagtag
    42841 agaatatgta aaagcgttga atctggtata taaacagcta atgtttgaag agttgataaa
    42901 agatgcaatt aaagaaatgg atcaaggtta tgctgagaga gaagaaagta ttcaaaatcg
    42961 gattgaaaag ttagcatata ctgatccaat agatcaaaaa aaacttgata aagcaaaagc
    43021 tgaattatct aaattgagaa atgaaaaaaa taaagaagcg agagaaatat taacgcaagc
    43081 gatagggttg aagagtcaaa cgagaacacg agatgtagca aaaaaattag actttgactt
    43141 tggagatttt gttgattccg caaatatgaa agctagtaaa gattttggca atgattttat
    43201 cggaattgtg accggacaaa gaacgcctag aatatataaa aatagaaaca aatatggatt
    43261 actacctttt agaggtcgag atattaaaat ccataaagat gataagtttt atatttattt
    43321 acctaaaggc tttaggttta aagtgaattt aggtagcaag ccaagaaaag caagagacgt
    43381 tgcttcagtt ttacaacaca ttatggatgg cgattacaaa ttaaatcaaa gtaatcttat
    43441 ttttgatgga agaaaattat tttttaatct aacaatttct tttgaaaaaa gctataatga
    43501 aatggaattc aagaaaggta atgaattgat cgtaaacttt gcagatttgt gtagcggtga
    43561 atgtttaatt aatgataaat ctgtaatgtc ttttggtgat agccaatata taagaaactt
    43621 caaaagtaaa attttagcaa tgaaatcaaa agaacaaagc cgaggagtct tttctaaggg
    43681 cggtcatggt cgaaaaagaa aattagatca agatcgatgg gaagcaataa agaaaagaga
    43741 aagtaatttt gttaaaactt ataataatca atgttctgca aaaatcgtta aaactgctat
    43801 tcgcaataaa gctcaaatta ttcgtatcat tgaaccagat aaggcttatg gcgaatgggc
    43861 ttactatcaa ttgaaagaac aactagtaaa caaaggaaaa agatatggga tagaagtcag
    43921 aagttagtaa attaaacaat cgttgatttg gcaacgaaat agtgagggaa aatccactca
    43981 ctgaagggta aactccgtca agttgtgtga ccagacggaa ggacgaatgt tgttgaggtt
    44041 ttagaaccat ataaaacagc atgtttctca aatttttagg ataagggaaa ttgctagcag
    44101 tttgttttag aaccatataa aacagcatgt ttctcaaatg agtactttaa attaattgat
    44161 ggcgttcctg ctttagaacc atataaaaca acatggattt aaaacaagaa gtaagtcagt
    44221 tctcctttta ctttagtaag gaaaaataaa gtattctcaa taaaacttaa aataagcaaa
    44281 cctttgtttt aatagctaat cgaagtaaaa cccaaagcca tatgaacccc tgaatcagcg
    44341 aaagtttatg atgatttgga ttactaatag tttaggaatt agatggtaaa tctaaattaa
    44401 acaatcgttg atttggcaac gaaatagtga gggaaaatcc actcactgaa gggtaaactc
    44461 cgtcaagtta tgtgaccaga cggaaggacg aatgttgttg aggttttaga accatataaa
    44521 acagcatagt tctcaaattg tttaaaatga ttgtcaagtt aaaaaaatct gggatcatat
    44581 aaaatagtat agttctcaaa tatgattaga aaccctatta aagagcaata ttaagccaac
    44641 ttacttaaat cgtttaatta agaacaataa tctcttttag tcagactttt agtctgactt
    44701 ttttgtattt ccaaaattta ttctttacaa aaacgaacat acgtttgtat aattgcttgt
    44761 gaatggggga ataaaaatgt ttgattacag tagagaaccg gtgaatgact attttttaat
    44821 cgacatgaag tctttctatg ctagcgtgga atgtattgaa cgaaatttag acccaatgac
    44881 tacaccgtta attgtcatga gtagatcgga caacactggt tcaggtctga ctctagcttc
    44941 ttcacctact gcaaagaaaa aatatcatat tactaatgtt agtagagcta ctgatttacc
    45001 ttatccatta ccaaaagaac ttcatattgt tccaccacga atgaacttat atataaaaaa
    45061 aaatatggaa gttaatcaga tttttagaaa gtatgtcgca gatgaagatt tattgattta
    45121 ttcgattgat gaatcaattt taaaagtgac aaaatcctta aatttattta ctagttcagg
    45181 aacaagaagc gagagaagaa gagaattagc taaaataatc aaagatgaga ttcagaaaga
    45241 aacaggtcta atagcaacag tcggaatagg agataaccca ttattagcta aacttgcact
    45301 ggacaatgaa gcgaagagta gtaaagaatt tatagctgag tggacttatg agactgtacc
    45361 tgaaaaaata tggaaaataa acgagatgac tgacttttgg ggcattggat cacgcatgaa
    45421 aaaaagattg aatcatatgg gaattatgag tatagaagat ttagcaaatt ggaatcccta
    45481 cacaattaaa agtcagttag gtgtaatcgg actccagtta tatttccata ccaatggaat
    45541 tgatcgtacg gatattgcta ttccaccaga accaaccaaa gaaaagtctt atgggaattc
    45601 acaagttcta ccaaaagatt atacgaggag agaagaagta gaactagtag tcaaggagat
    45661 ggcagagcaa gtagccattc gaattagaca acacaattgc aaaactggtt gtgtacgctt
    45721 agcaattggt acgtcaattt tggaagaaaa atcaggtttc tcgcaccaga tgaaaatacc
    45781 aattacagat aatacgaaag aattacaaaa ttattgtctt tttttattca ataagtttta
    45841 tgaggggcag gaagtacgac atataggagt aacttatagc agattagtat acacagattc
    45901 cctacaatta gatttattta gtgatccaag aaaaaagatt gctgaagaaa atttggatag
    45961 gattattgat aagattagac aaaaatatgg atttacagct attatacatg cttcatctat
    46021 gttagaaggt gcaagaagta ttacgagaag cactttaata ggaggacacg caggaggatt
    46081 aggaggtttg cataatgatt gaatataacg atagaggacg tttaaaatgg tcgggatttt
    46141 atttgtctga tcatactgaa agaatcaatg cagacaatgt acaacgtgct aatcataatt
    46201 tggcaaaaga acaaatgacg actgaagaga ttgcgaaaat actgagaact gctgtcataa
    46261 aaaatagacc cgtctgcatt caaaaagagg aacgaaacgc tgaaggttac tatccagatg
    46321 atgtagtagg aaaaattatt ggatatgacg agcttggtgt atttgtggaa gaagaaaaag
    46381 ttcactatga tgaaattagg aatgtttctt tctcggaatt attaaaatgg agtcagatat
    46441 gaacattatt aaaaaatacg aattcggtta tattacgtat gaacagctaa tagaggaatt
    46501 atggggatat ggtcaacaat taattaatga agtggggatt gattgctttt gtttctatat
    46561 tgaatccgga gcaggatacc ataagaacag atactatata tttccatatc caccaaaata
    46621 gtctcttaga tatttttaaa ttttctccaa atactttcaa ttattgtgaa atatttccct
    46681 ttaattaatc aaatttttta taaaagtaat atataaaata aattgcttga agtattatat
    46741 ttagatatca taatttatgg aagaaaaatt aattaaaaag gatgagatta taatggggtt
    46801 actggttccg agagctaata cttatactat acctttaacg aaacgccaat taattgctaa
    46861 aagatatcaa aggattacta gagccatcaa tagagagttt tggaattctg aaagtgatac
    46921 tgctcacagt ttatacgtag gttcttatgg gagaggaacg gcaattagta ctagtgatat
    46981 agatataata gttgaattac cgatggcaga atttgacaga ttcaaaaatt atttatcaaa
    47041 tggcccgtct aagttattac aagttataaa aaacgcattt caggaaatac taccaaatag
    47101 tgatattaga gctgatggac aagttgttaa aataaatttc catgatggaa tcaaatttga
    47161 aatagttcca gcttttaatg aaaaagatta ttggggtgag tcaaaagggt ttatttatcc
    47221 agattcaaac atgggaggga actggaaagc tacaaatccc aaaaaagagc aagaagccat
    47281 gaaattaaag aatacaaaaa gtaataattt attatatgct acgtgtaaac attttcgaca
    47341 tgtacgagat acagaattta cgagttatca tctttcagga atagtaattg acagtttcgt
    47401 atatgaggct atgggaaatt ggaaattcgt tgaaaataac agtgggggtc aaaacattag
    47461 ttctgtatct tatgagacag ctttattaga atactataac tcacataaag ttatgggcgg
    47521 tttaaattta tactctcctg gaagcaatca atttgttaat tctgatagta gtatcatatg
    47581 tttagaaaaa gtattaaaaa aaatagcttt ataaagtttt atggaggaac aacatgggag
    47641 acgaaaaaaa gcgcttggaa gttcaattac gtgaagcata tggacgagta acgtatactt
    47701 acaccactca tttaaagcaa gccgataata ttatttctga caataaaatt ttaaggtgtc
    47761 ttcaaattgg tttgtcagcc cttactactg gaggggtact tagtaccatt attactgata
    47821 atagtgagtt aacctttgtt gctggattat gttcaacaat ttctcttgga ttaaacttat
    47881 actttaagaa ttttgatctt gctgaagaag ctaaaaatca tcaaattgtt gccgataaat
    47941 tatggttgat cagagaagaa tacgtaagct tactaacaga tttaaatcgt ttaagtgttg
    48001 aagatattgt gaaaaaaaga gatcaattaa tgaacgaaac tcatgaagca tataaaaatg
    48061 cccctaaaac aaatagtaaa agctatgaat cagctcagaa agcattaaaa tctgaagaag
    48121 agcaattttt tacagaagag gaattggata agatgttacc agagcatctt agaaaaaaga
    48181 gttctaaata ttgaactctt tttttatgct taaaattctc ttgtaatttt tctattcatc
    48241 tatatttaag aagaaatatg atgatagaaa catctcctaa agccaccgca agccgttcta
    48301 agcatagctt agcccgacat tgctagatat gctcatctca ccatgaacgt ttcctaaagc
    48361 caccgcaagc cgtcctagcc attgcctagt ccg
//
LOCUS       DACKAC010000014        45771 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.014, whole genome shotgun sequence.
ACCESSION   DACKAC010000014 DACKAC010000000
VERSION     DACKAC010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.014"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            9..731
                     /locus_tag="I0B46_000851"
     CDS             9..731
                     /locus_tag="I0B46_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAR2083171.1"
                     /translation="MLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEPKSIPTTLNVAL
                     IGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGMNKINAAYSYGG
                     VDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSLDLDGVYINKGE
                     QVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFKLPKAVGKLLGS
                     IQTNLPESVLLDCGMDFLKDIC"
     gene            789..2828
                     /locus_tag="I0B46_000852"
     CDS             789..2828
                     /locus_tag="I0B46_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAR2083172.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFQTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLINPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(2937..4088)
                     /locus_tag="I0B46_000853"
     CDS             complement(2937..4088)
                     /locus_tag="I0B46_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAR2083173.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4242..4772)
                     /locus_tag="I0B46_000854"
     CDS             complement(4242..4772)
                     /locus_tag="I0B46_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083174.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(4871..5221)
                     /locus_tag="I0B46_000855"
     CDS             complement(4871..5221)
                     /locus_tag="I0B46_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAR2083175.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5378..6907)
                     /gene="ahpF"
                     /locus_tag="I0B46_000856"
     CDS             complement(5378..6907)
                     /gene="ahpF"
                     /locus_tag="I0B46_000856"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAR2083176.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(6917..7480)
                     /gene="ahpC"
                     /locus_tag="I0B46_000857"
     CDS             complement(6917..7480)
                     /gene="ahpC"
                     /locus_tag="I0B46_000857"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAR2083177.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(7624..8286)
                     /locus_tag="I0B46_000858"
     CDS             complement(7624..8286)
                     /locus_tag="I0B46_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAR2083178.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            8470..10116
                     /locus_tag="I0B46_000859"
     CDS             8470..10116
                     /locus_tag="I0B46_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAR2083179.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            10107..11189
                     /gene="uxuA"
                     /locus_tag="I0B46_000860"
     CDS             10107..11189
                     /gene="uxuA"
                     /locus_tag="I0B46_000860"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAR2083180.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNEYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(11297..12850)
                     /locus_tag="I0B46_000861"
     CDS             complement(11297..12850)
                     /locus_tag="I0B46_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAR2083181.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQND
                     FMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQ
                     SKKENQVTS"
     gene            12998..14167
                     /locus_tag="I0B46_000862"
     CDS             12998..14167
                     /locus_tag="I0B46_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAR2083182.1"
                     /translation="MRYSTILISTFAALSLSLTINQTVHTEEIETAKWTETKWANVNR
                     ITFQDEFSSFNSGVAKLRKGQTATIKAKIDYSGDLAPILQSAVTFENVDPALSIGYDE
                     NTLTIQHKQAIFTFTVTLNQDLLKPALFTVKVSDGLPNDHHHLTPYSQRQTIEQDSAI
                     DSGGDLVEEPTDKPENENKPEVPPTENPDGEQKPEIEPGEEPDTETQPEPDNESKPEI
                     TPGEKPDVDPEEKPDVTPEPDTDSGNQTVPETNPDTDNETENPEKLEVDPEEKPDVTP
                     EPDTDARDQGIPEKINKKTIQEDGKKESKKSNLAILKINEEQLNKKSRIFDSAQSAET
                     LKSSKDTTFASPETKNKQLPKSGESQNKVILWSGIILLSIATMLSAKRFKQNRSL"
     gene            complement(14293..15372)
                     /locus_tag="I0B46_000863"
     CDS             complement(14293..15372)
                     /locus_tag="I0B46_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAR2083183.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVIALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(15481..15921)
                     /locus_tag="I0B46_000864"
     CDS             complement(15481..15921)
                     /locus_tag="I0B46_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAR2083184.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(15978..16388)
                     /locus_tag="I0B46_000865"
     CDS             complement(15978..16388)
                     /locus_tag="I0B46_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAR2083185.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(16569..17003)
                     /locus_tag="I0B46_000866"
     CDS             complement(16569..17003)
                     /locus_tag="I0B46_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAR2083186.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(17103..17951)
                     /locus_tag="I0B46_000867"
     CDS             complement(17103..17951)
                     /locus_tag="I0B46_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAR2083187.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            18340..20106
                     /locus_tag="I0B46_000868"
     CDS             18340..20106
                     /locus_tag="I0B46_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAR2083188.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            20208..21467
                     /locus_tag="I0B46_000869"
     CDS             20208..21467
                     /locus_tag="I0B46_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAR2083189.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            21614..22915
                     /locus_tag="I0B46_000870"
     CDS             21614..22915
                     /locus_tag="I0B46_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAR2083190.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            22918..23769
                     /locus_tag="I0B46_000871"
     CDS             22918..23769
                     /locus_tag="I0B46_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAR2083191.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            23782..24597
                     /locus_tag="I0B46_000872"
     CDS             23782..24597
                     /locus_tag="I0B46_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAR2083192.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            24748..25914
                     /locus_tag="I0B46_000873"
     CDS             24748..25914
                     /locus_tag="I0B46_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAR2083193.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            26144..26791
                     /locus_tag="I0B46_000874"
     CDS             26144..26791
                     /locus_tag="I0B46_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAR2083194.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            26875..27819
                     /locus_tag="I0B46_000875"
     CDS             26875..27819
                     /locus_tag="I0B46_000875"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAR2083195.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            27954..29405
                     /locus_tag="I0B46_000876"
     CDS             27954..29405
                     /locus_tag="I0B46_000876"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAR2083196.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            29426..30262
                     /locus_tag="I0B46_000877"
     CDS             29426..30262
                     /locus_tag="I0B46_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAR2083197.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            30262..30921
                     /locus_tag="I0B46_000878"
     CDS             30262..30921
                     /locus_tag="I0B46_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAR2083198.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            31047..31736
                     /locus_tag="I0B46_000879"
     CDS             31047..31736
                     /locus_tag="I0B46_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083199.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            31742..32698
                     /locus_tag="I0B46_000880"
     CDS             31742..32698
                     /locus_tag="I0B46_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAR2083200.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            32742..33254
                     /locus_tag="I0B46_000881"
     CDS             32742..33254
                     /locus_tag="I0B46_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAR2083201.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(33510..34058)
                     /locus_tag="I0B46_000882"
     CDS             complement(33510..34058)
                     /locus_tag="I0B46_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAR2083202.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(34147..35328)
                     /locus_tag="I0B46_000883"
     CDS             complement(34147..35328)
                     /locus_tag="I0B46_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAR2083203.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(35564..37072)
                     /locus_tag="I0B46_000884"
     CDS             complement(35564..37072)
                     /locus_tag="I0B46_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAR2083204.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            37231..37422
                     /locus_tag="I0B46_000885"
     CDS             37231..37422
                     /locus_tag="I0B46_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083205.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            37625..38542
                     /locus_tag="I0B46_000886"
     CDS             37625..38542
                     /locus_tag="I0B46_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAR2083206.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            38535..39608
                     /locus_tag="I0B46_000887"
     CDS             38535..39608
                     /locus_tag="I0B46_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083207.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            39756..40274
                     /locus_tag="I0B46_000888"
     CDS             39756..40274
                     /locus_tag="I0B46_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083208.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            40255..40647
                     /locus_tag="I0B46_000889"
     CDS             40255..40647
                     /locus_tag="I0B46_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAR2083209.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            40723..41658
                     /locus_tag="I0B46_000890"
     CDS             40723..41658
                     /locus_tag="I0B46_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAR2083210.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            41791..42570
                     /locus_tag="I0B46_000891"
     CDS             41791..42570
                     /locus_tag="I0B46_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAR2083211.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            42563..42880
                     /locus_tag="I0B46_000892"
     CDS             42563..42880
                     /locus_tag="I0B46_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAR2083212.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            43124..44734
                     /locus_tag="I0B46_000893"
     CDS             43124..44734
                     /locus_tag="I0B46_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAR2083213.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            44891..45739
                     /locus_tag="I0B46_000894"
     CDS             44891..45739
                     /locus_tag="I0B46_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAR2083214.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT        15181 a         8248 c         8803 g        13539 t
ORIGIN      
        1 tatctatttt gttactgctt acattagtag ttggctattt ttcgattgag tttatccatg
       61 gattttcgtc tgcaaaacag agttcaactg tgaaaaaggt agagccgaaa agtatcccta
      121 ccacactaaa tgtggctttg attggttcag atgctcgttc gaaagacgaa aacggtcgct
      181 cggattcact tctggttgca caatacaacc agaaaacaca acaagcaaaa ctgatctcta
      241 tcatgagaga ttcatatgtc gatattccag gttacggaat gaataagata aatgcagctt
      301 actcttatgg gggagttgat ttattaaatc aaacattaaa ggaaaatttc aaatttgaag
      361 ctccctatta tgcaagtatc acatttcaag attttatcga ttgcgttaat gaactgtttc
      421 ctggtggagt caagatcgat gcagaaaaat ctctagattt agatggcgta tatataaaca
      481 aaggagagca agtgatggat ggtaatactt tactccagta tgctcgattt cgggaagaca
      541 aagaaggaga cttcgggagg atcagaaggc agcagcaagt gatcaaagct atctctcagc
      601 agctgaaaga cgtaacttca atattcaagc tacccaaagc ggttgggaaa ttacttggta
      661 gtatccaaac gaaccttcca gaaagtgtgc ttctggattg tggtatggat ttcctcaaag
      721 acatatgtta gaataaacag gtataaatag tgaaataaag gaatgacaag caagagaagg
      781 aggaaaaaat gaaaagaagt gacaagcacg gcaaaaatcg aacaggcgct tatattgccg
      841 gcgcagtgat cttaatagca gctgcgggtg gcggttattt ttactaccag cactatcaag
      901 aaacccaagc agtagaagct ggagaaaaga cagttgagca atttgtccaa gctttgaaca
      961 agggagatta taacaaagct gcagaaatga catcaaaaaa ggcggcaaat aaaagtgcat
     1021 tatctgaaaa agagatctta gataaatacc agaatatata cggtgctgct gatgtcaaag
     1081 gacttcagat atcgaatcta aaagtagata aaaaagacga ttctacttac agtttttcat
     1141 ataaagcaaa gatgaatacc tcattaggtg aattgaaaga tctttcttat aaaggaacat
     1201 tagacagaaa tgatggtcaa accacgatca actggcaacc caacttggtt tttccagaaa
     1261 tggaaggaaa tgacaaagta agcctgacca cacaagaagc agcaagaggg aatattatag
     1321 atcgaaatgg tgaaccatta gcaacaacag gcaaactaaa acaattagga gtcgttccaa
     1381 gtaaacttgg agatggaggc gaaaaaacag ccaatatcaa agcgattgct tcctccttcg
     1441 acttaacaga agatgctatc aatcaggcga tttcacaaag ctgggtacaa cccgattact
     1501 ttgtcccatt gaaaatcatt gatggagcaa cgccagaact tccagctgga gctaccattc
     1561 aagaagtaga cggcagatat tatcctttgg gtgaagcagc tgctcagctg attggttacg
     1621 tgggagatat cacagcagaa gatattgata agaatccaga attaagcagt aatggaaaaa
     1681 tcggtcgctc aggtttggag atggcttttg ataaggatct tcgtgggaca acaggtggga
     1741 aattaagcat tacagatgca gacggtgtag agaaaaaagt tctgatcgaa catgaagttc
     1801 aaaatggcaa agatatcaaa ttgacaatcg atgcgaaggc acaaaaaaca gcttttgaca
     1861 gtctaggagg aaaagctgga tcaactgttg cgacaacgcc aaaaaccggt gatcttcttg
     1921 cgcttgctag ctctccaagc tatgatccaa acaaaatgac aaacgggatc tcacaagaag
     1981 attacaaagc ttatgaagaa aatcctgaac atccattcat cagccgattt gcgacaggtt
     2041 atgctcctgg atctacgttt aaaataatta cagcagcaat cggtctcgac aacggcacta
     2101 tcgatccaaa tgaagtgttg acgatcaacg ggcttaaatg gcaaaaagac agttcttggg
     2161 gatcttttca aacaacgcgt gtcagtagtg atgtatcaca agtagactta aaaactgctt
     2221 tgatctattc cgataatata tatgcggcac aagaaacgtt gaaaatgggt gagaaaaaat
     2281 ttcgtacagg cttagataaa ttcatttttg gtgaagacct tgatttgcca attagtatga
     2341 atccagcaca aatttctaat gaagatagct ttaactcaga tatcttgcta gctgatactg
     2401 gatatggaca gggcgaactt ctaattaatc ctatccagca agcagcaatg tattctgttt
     2461 ttaccaacaa tggcacactt gtctatccta aattgattgc agataaagag acaaaagata
     2521 agaagaatgt catcagcgaa acagcagtac aaacgatcgt gccagatctg agagaagttg
     2581 tgcaagatgt aaatggtaca gcacattctc tttctgcttt agggattccg ttggcagcga
     2641 aaactggtac agcggaaatc aaagaaaaac aggatgtaaa ggggcaagag aacagtttct
     2701 tgtttgcttt caatcttgat aaccaaggtt atatgatggt cagcatgttg gaaaataaag
     2761 aagatgatga ttcagcaacc aaacgagcat cggaactatt gcaatacctc aaccaaaatt
     2821 atcaataaaa aattgctttt taagtacagt aaaaggactg tggcgttatc gtcacagtcc
     2881 ttttttcaaa taaacggttt tcacgatgtc tctttcacaa cttctttttg atttactcac
     2941 acttcataaa ctttttttgt aaaatatttg agaacaagtg gagaaataag tgtacttaat
     3001 aaaatcacga taactaaagg tgaatagtac tgtggttcga tcaattgact ttgctgtcca
     3061 atttgtaaaa tgatcaaagc catttctcca cgcgaaatca taccagcacc gaccatcaat
     3121 gaactatgtt gagaaaaacc ggcaatctga gcaccaaaat aacctccaag caatttggtc
     3181 aaaatcgcta caagcgtcaa tatagaaata aagagtagtt gttcactaaa gtcagaaaaa
     3241 tcaacttcta atcctacact gacaaaaaat actggtataa atactgcata tcctaaagcc
     3301 tctacattgt ggtatacttc ttctttaacc tttgtctgac tgacagctat cccagcaaaa
     3361 aaagcaccaa tgacagagct aagcccaaca agatccgcta cgtaagacat tcctaaacaa
     3421 ataactagcg atacgatgat aatagaagaa tttgcgtaca ctttttctgc aaatgaaagt
     3481 aggaaaggcg caatccattt gactaataag aaaataaaaa caaaataaat tagctgttca
     3541 atcaaaacaa gcggtaaaga agcattgcta gaagcttccc ccgttagaaa agataggctg
     3601 aaacttagaa ctaaaacgac taaaatgtca tctaccacag aagcacccaa aattgttgat
     3661 ccttcttttg tattcactac gtttagttct tttaatactt ctactgaaat actgacagaa
     3721 gttgccgcaa gaataatccc aaagaaaatc gcttcatttc ccgaaacttg gaatgctttg
     3781 cctgtcaacc agccaaaaac aaccgggaag atgatcccaa gcaaagcaac gaacatacca
     3841 ggtcggaaat attttttcaa aagagataaa tcactttcca gaccagctaa aaacattaat
     3901 aagatcacac caatctccga aaagtcatgg actagaatat caggtttgac ccaattcaat
     3961 ccagcttggc ctagcaaaac accaacaagt aattgtccaa tcactgctgg gatacctaat
     4021 ctccgcgaga tatgtgagcc aatcgttgtt gaaatcaata tcaggcataa aataccaata
     4081 aattccattc tcatcattcc tttgtataaa atatagcccg aacttagcat aacatacatt
     4141 attgaaaaaa taggaaaata tactcacaaa aaaaccaaca atccaaaaga tttttttgga
     4201 ttgttggttt ttatacggtc tattttaata actaaaaatg attaacgtat agtcattttt
     4261 cttattctga tgaaattgat aatctcctgg atgcccatga aaatcaaaat aaagccgaat
     4321 atttggaata gaagcatcaa acttttgaat ggattgaaca ataagacgaa tccgccaatc
     4381 aacaacagaa tactactata aacactccaa cttttagctt ttgtattcat gctgaagaac
     4441 aactggaaca agctgttgat gataacagct aatccaagaa gaatcggcag gatagaaacg
     4501 attgcaggtg caaaagcata gacgatcaga gctaagatca ggaaaaaaag accactgaac
     4561 agaccgatac tccaagaacc tgttttcctt ttaaaacggt aagcctcaaa aagattcaac
     4621 aacccaagca aaacaagata ggcggtgatc agataaccga taaaatgaaa aacagcagaa
     4681 gggtttagca caatcgcaat cccagaaata atataaacag cagctcttaa taaggcgtat
     4741 ttttgaatat tttgtttaat cgtctcaaac atataagaaa acctcctttg acttatttta
     4801 acgtgaacta gtcattttac caaattaaat gttacaaaat gagcgaagga gaagaatatc
     4861 tctggaaaaa tcaacttctg aggatctttt ccattttttt gccttttgct agctcatcga
     4921 tcattttatc taaataacga atttctttca tcaagttatc ctcgatgtct tctactcgta
     4981 caccacaaac aacacctgta atcaaggaac gtgaaggatt ggggacaggg gcttccgcaa
     5041 aaaattgttc gaaagtcact tgtttttgta gttgatattc tagttctgtt tgggaataac
     5101 ccgtcagcca acaaatgatt tcatctactt cctcttttat acgatctttt ctctccgctt
     5161 ttgctacata cataggataa acgcttgcaa aacttgtcgt aaaaatacga ttttgcgcca
     5221 taatcagtca cctttctttt tttcaaatta tttactactt atagtatata agaaaaagag
     5281 aagaaaaagc gattattcaa aaaaacaaaa aggattgtga caaaattctt gtcaccatcc
     5341 ttttttccgc tctctgactt tttacaatct tcatagctca agtgcgcatc agataatcga
     5401 atgctcctaa agctgcagta gctcctgaac ccattgaaac aatgatttgt ttgtatggac
     5461 tatctgtaca atcaccagct gcgaaaacgc cggggatatt cgttgcgcca tttttctcgg
     5521 tcactatttc atttcttgga ttcatttcga tcgttccatc tagccaatcc gtattaggga
     5581 gtaagccgac tagaatgaat actcctgaaa cagctagtgt atgcatctcc atcgtacggc
     5641 gatcgatata ggagagagat tctacctgat tcgtaccgct gatttctttc gtcgctgcat
     5701 gtttgataac agtgacattc gcaagagaga ctagtttatc ctgtaagatt tgatctgctt
     5761 ttaattctgg taagaattct agaacatata catgttttgc taacccagcc aaatcgattg
     5821 ctgcttcaac accagaatta ccaccaccga taacagcaat ctcttttcct ttaaataaag
     5881 ggccgtcaca gtgtgggcaa taagcaattc ctttattttt aaactctttt tcgcctggta
     5941 cattgataga acgccagtgc gcacctgtcg cgatgatggc ggtttttgct ttaagtgata
     6001 taccgttagc taagccaata ttaataaaat gttgctcttt atttaaggaa acggcctgtt
     6061 gattcatcat aatgtcgata gggtaactac gcacatgact ttctacttgc tgcatcaatt
     6121 ttggaccttc tgtgtaagag gtaccaatca tattttcaat ccctagtgtt tcaacgactt
     6181 gtccgccgaa agaatcagcg acaagtcctg tacggattcc tttacgtgct gcatagatag
     6241 ctgcactact agctgctggt ccaccgccaa ttactaatac atcataaggt tctttttcaa
     6301 acaactcttc ttcaatcaaa gggccagtga ttttttcaac aatttgttcg atcgtcatgc
     6361 gtccgctact aaattcttct ccatttaaaa acacagttgg cactgccatg atttttttat
     6421 catcgatttc tttttggaac atcccacctt caatcatcgt atgagtaata tttggattta
     6481 atacagctaa tatattcaat gcctgtacaa cgtcggggca attatgacaa gtcaagctaa
     6541 cataactgtc aaaatgcaat gtttgtgtta tttttttgat tttaagactc aaagattcac
     6601 tgattttcgg tgcccgtcca ctaatttgta acatagccag taaaaaagag gaaaattcat
     6661 gaccgagggg gataccagca aaaacgattc cactttcttt gccaactgga ttgatagaaa
     6721 aactaggtgt gcgcatcgaa tctctttttt cccaagagat tttaggagac atttctgcta
     6781 tttccttcgt aaattcagct agtttttgac tattttcact attatccaaa ctaacagaaa
     6841 aaacaatagg agattcaatc agtgctaaat attgttcgag attttgttta gtttctttgt
     6901 ctaacatatc caccacctaa atctttccaa caagatcaaa acttggtttc aatgtttcgc
     6961 cactttcttt ccattttgcc ggacaaactt cacctgggtg ggtacgtaca tattgagcag
     7021 cgcgaatctt atcaatcagt gcactagcgt cacgaccgat tccatctgca ttgatttcca
     7081 tagcttgaac gattccgtct ggatcaataa taaatgttcc tcgctgagct aatccttgct
     7141 cttcatccaa aacatcaaaa atacgagaaa tctgatgaga cggatcaccg atcatcacgt
     7201 attcgatttt accgatagca tctgatgtat catgccatgc tttgtgagta aagtgggtat
     7261 cggtagagca agaaaagact tcaacaccta aagatttcaa tgtactgtat tgttcttgta
     7321 agtcttctaa ttcagtagga caaacaaaag taaaatcagc tggatagaag caaagaatac
     7381 tccagttccc cataagatct ttttcactga cttctaaaaa ttctccttga tgataagcat
     7441 gtgcattaaa ttcttcgatt tttttcccaa tcaatgacat agtaaaacct ccaataataa
     7501 ttatttattt ataattattg taatttagtt ggagttgcaa agtcaatgga tattttgtta
     7561 tttaataaag attactcaaa aacgaaggtt aattataaaa atagaagagg tcgcaagaaa
     7621 atgctactgc tggaaatact cgggataacg ttgagtaaca gtttcctgtt ctttcagcat
     7681 catgtgtaag tgcaatcccg tttgaaatcg agcttcttct ggttttttat caactaaagc
     7741 cttgagtaaa tcataatgtt gttccatgat cgtttcccat ggaagcccct gaatggtcac
     7801 tcgtaaccag cggaaacgtt ctaaatgggt attatggtca tctaaccagt tccaaacttc
     7861 ttttcttcct gcaatttcga aacaagcttc atgaaataaa ttatctgcac ggaaaaaagc
     7921 gcgttcgtca tttattttga ctgctttttc ttgttcttct aaaatcgttt ctagcactat
     7981 tcgtgtttgt gtgtccaatt tggaacaaca ttcaatcatg atttgctctt ccaactgttc
     8041 tcggacaaag cgagcatttt ctgcagaaac aagattaatt ttggaaacat acgtaccact
     8101 ttgaggtatc gtatacacga gttcttgctg acgtagttga atcaatgctt cgcgaatggg
     8161 agtccggccg atagcaagat gttcctccag tgccttttca gatattttcc ttccaggttc
     8221 taagtcagca tagataattt gtttacgaat cgcttgataa gcttgtgatt gcaagctgct
     8281 tttcatcttt attcctccaa aatcatttct atgaacgtag tataacagaa gaggtctgaa
     8341 aaacaatctg gtatgtcaca ttaactagta aatttgtata aatagtaaaa ttttggttga
     8401 catagcgatt tttattgtat aaaattcact tgaatacaaa aactaatatg ttagtttagg
     8461 aggggaaaaa tgattgattt aaacaactgg aggaacttta tccctgaatt atctcaaaaa
     8521 gaagtagaaa tacctcattt tgaccgtgca tcattaagag aaaatggaca aaaaaatcct
     8581 gtctggatcc atttcggtgg cggaaatctt tatcgaggat ttcatgcaga aatcgctcaa
     8641 cgtttagcgg atctaggcga attgtccact ggtgtcattg tttgtgaaac attcgatgaa
     8701 gaaataattg aaagggctta ccaagatttt gatgatttga ttcttgaagt ggtcatgcca
     8761 agcagtggag agttcgtcaa acgtatatta gctgcgacag ctgacagtct gtattgtcat
     8821 ccagattgtt ctgaacactt tgataagatg attcgacact ttgaaaatcc tagtttacaa
     8881 ttagttactt ttgctattac tgaaaaaggt tatcgattga aagatacaaa taataaatat
     8941 ataggaccga ttgcagtcga tataaaaaat ggtccaaaga agccatcaca tacaatgagt
     9001 attcttgctg cgctacttta taaacgtttt ttatctggag cttggccgat tgcacttgtc
     9061 tctacagata atttttcaca aaacggtagt caattacgta cttctttgct aacgatcgca
     9121 aaagaatggc aaaaggaagg ttatgtacaa aatacattta tccaatactt atcgaatgaa
     9181 gaaattgttg cgtttccatg gacgatgatt gatcgtatca ccccgaatcc ttccttgtca
     9241 gttagcaaaa tgttggcaaa agagggcttt tcaaatatgt cgattttcca aaccgcaaaa
     9301 ggaaataata ttgctccatt tgtcaataca gaggacatcc attatttaat catggaagat
     9361 cattttccca atggacgtcc gaaattcgaa aaagctgggg tattcttgac tgatcgtgca
     9421 acggtagata aagcagatac gatgaaggtg acgacttgcc ttaatccttt gcatacgtct
     9481 ttagctatta tgggttgttt actggggtat aggagcatag ctgaggagat gaaagatcca
     9541 gatttatcgc tgctagtttc tgaaatcggg tacaatgaag gcctcccagt tgtggagtct
     9601 ccaggtatca ttgatccaaa gatctttctg gaagaagtaa ttactgtgcg cttttctaat
     9661 ccaaatattc ctgatatgcc tcagcgaatc gccacggata cctctcaaaa ggtggcgatt
     9721 cgttatggtg agacgattaa aaaatatatg gaaatagaag ttaaagaccc gcagacactt
     9781 gtattcatac cattgactct cgctgcttgg cttcgatacc ttattggcat tgatgatcaa
     9841 ggaaaaccat tcatcccgag tccagaccca ctattgacag actttcagca taagctttcc
     9901 gatattcaac tgggtacgca agatacatca catattcatg agaaaatggc tcctattttg
     9961 tcaaataaag aaatttttgg tgtagatctt taccaagcag agcttggcag aaaaatagaa
    10021 gaaatgttca tcaagatggt agcaggaccg aatgccgtac gaaaaacatt acaggattac
    10081 ttacaagaac atggaggaaa tttatcatgg aaatgacttt tagatggtac ggatcacagg
    10141 aagaaaagat taccttagat gaaatcagac agattcctgc ggtcaaaggg attgtaggca
    10201 cattgatgga tataccagta ggagaggtat ggcccaggga gaggatcaaa gaactaaaag
    10261 aagaaattga gtctcgtggg ttaacactaa aagtaattga aagcgtcaat gtgcatgatg
    10321 ccattaaaat tggattacct tcccgtgagc agtatatcga aaactataag cagacaatcc
    10381 gaaacttggc agaatatgga atagaagtca tctgctataa ttttatgcct gtatttgact
    10441 gggtgaaatc tgaccttgac taccgcttag aagacggctc atccacgtta gcttttatat
    10501 ctgatgatat tccagatgat ccaagagaaa tcgtcgcgcg catcgaacat tccagcaatg
    10561 aatttgaatt gccgggatgg gaacctgagc gcttagctca tatcaaatcc ttgcttgaag
    10621 cctatgcatc agtagacgaa gaaaaacttc gagaaaattt tgcctatttt ctccaatcaa
    10681 tcatcccaac atgtgaagag gtaggggtca aaatggctgt acatccagat gatccgccat
    10741 actcgatttt tggattacct cgaatcgtaa agaatcgaga agatttagat tggttgtgcc
    10801 gcatagtaga cagcccttat aacggaatca ctctttgtac agggagtatt gcagaagatc
    10861 cagaaaatga tgtatatggg attttagcag aattttgtca aagagatcga atcccatttg
    10921 cacatgttcg aaatattaaa tttttagcta atcgagattt ctatgaggca ccgcataaat
    10981 ccgaatacgg ttcattagac atgtaccgca tactaaaaac aatgtatgac aacggattta
    11041 acgaatacat ccgtcctgat catggaagaa tgatctggaa tgagacagga agaccaggat
    11101 acggcttata tgatcgagca ttaggtgctg tttatctaaa tggattatgg gaagcaatcg
    11161 aaaaaggaaa caaaagtaaa gataattagt gaaaaaaact gtaattttat tatgtaaata
    11221 agaaggatgt tcaaattctt gatacataaa tccggaggtt gttaataagc attcggattt
    11281 atgtatctta tttggtctag gaagtaactt gattttcttt tttcgattga tccatgagtg
    11341 cttcttcttt taattcaaag atatattctg agatcctcat aatcactttt ttccagtctt
    11401 ctggtgtgat gattcctttt aaaatataga tttttttgat atgagaaggc aattgtttca
    11461 aagggcagtc gctgataacg atgttgcagt tctccataaa atcattttgt attttgatcg
    11521 attcatttga aaaaatttgt tctatcttaa cgatcagatc tttactgata taaaaatcac
    11581 gcgtatagtt cacaccaatc acaattcgcg gctgtttttt gtagcgatca tacaaatgta
    11641 ctaggtcttc aaccatccat tgcacatttt ccttttctaa aataactggt aacaagtgtt
    11701 gattttgttc tctgaaatcc atataaaatt gttcaatggc ggtttggact tctttcaaca
    11761 cgctatgttc ttgataaagc atgaaagtag gaaggggcgt atttggttga ttgaagttaa
    11821 gaaatccatt ataaagttta tttcgtgcga atgccaatga gtaaagaatc ttttcttttt
    11881 caggtataaa caaatcaaat acagtacaaa aggactccac catttctctt gaataaagaa
    11941 catgaggaac gtttgctttt tttaaaagtt tgtattgctc caagcttgtt tcttccgatt
    12001 cgattttcgt gatagaaagt cgagctaaaa tattcactaa gatgcgttgt tcgcgacaaa
    12061 ttggcagatt aggtcgcaaa atatctacag agggatcaat aaaaaagtca atatattcat
    12121 actctgtgaa ttctttttgt acgtctttca cagaagttgc aggaaaattc tctttacata
    12181 tttttaatag aaaatataat ttatctatca taccgctatt tagtcggtca agtacttctt
    12241 ttttaaaaaa agtattcatc ataaaagcca ctacttgcgg atcttcatca taaaattctg
    12301 tagcagtact tgaaaagatg ttccaataaa tatctaatgc tgcatattga atttgtacct
    12361 cttttccaga aaaagacttt ctgccattat tagaaaagga gatagacaaa ccgtacaatg
    12421 ctaaaaatgc attaatcttt ttcattttcg tatacaaata agaggcacta tagtttgttt
    12481 gctgacttaa actgataatc gaatttgtct gacagctgaa caaagaagtc aagatctgat
    12541 aactgactga ttcgttgcag tatcttccaa atagacgata atagcagtaa gccggatcaa
    12601 ttccttcaaa attcaacttt aaaaaaggtt tttgcttatc aatatgcagt tctgttagat
    12661 tgagacgctc aaagtcttgt tctaattctt tgatgtatag taaaaaattt gattcagaca
    12721 tatggaaatg atctgctaaa tattgtgtag taataaagaa acgatgttca gataggaaaa
    12781 gaaaaatatc taattttgta ttgctttttt tcttcaagag aaacgtttta atcgcttctt
    12841 gagtttgcat gaaagaggct cctttttact taaattgtta aaacttgaaa atattactgt
    12901 ttttttcata aatttagaca aaaaaacagt aaacatatag gtagaatctc tactatctta
    12961 tttcttgcat acaaaaaaat caaggagtga gagagttatg agatattcaa ccatcttaat
    13021 atcaacattt gcagccttaa gcttatcgct tactataaat cagacagtcc acacagagga
    13081 gatagaaaca gccaaatgga cagaaaccaa gtgggccaac gtcaatcgaa ttacctttca
    13141 ggatgaattt tcatccttca attcaggagt cgcaaaatta agaaaaggac aaacagcgac
    13201 catcaaagca aaaattgact attcaggaga cttagcacct atactacaat cagctgttac
    13261 atttgagaat gttgatcccg ctttgtcgat tggctatgac gagaacactt tgactatcca
    13321 acacaagcaa gcgatattta ccttcaccgt tacattaaat caggacttgt taaaaccagc
    13381 gctttttact gtcaaggtat cagatggact accgaacgat catcatcatt taacgccgta
    13441 tagtcagcgg cagacaattg agcaagattc agctatcgac tcaggaggag atcttgttga
    13501 agaaccaaca gataaaccag aaaatgaaaa taagccagaa gtaccgccta cagaaaatcc
    13561 agacggagaa cagaaaccgg aaatagaacc tggagaagaa cccgatacag aaactcaacc
    13621 agagccagac aatgaatcaa aacctgaaat aacccctgga gaaaaaccag atgtagaccc
    13681 agaagaaaaa ccggatgtga ctccagaacc agatacggat tctggaaacc aaacagtacc
    13741 ggaaacaaat ccagacacag ataatgaaac tgaaaatcca gaaaaactag aggtagatcc
    13801 agaagaaaaa ccagatgtga ctccagaacc agatacggac gcaagagacc aaggaatacc
    13861 agaaaaaata aacaaaaaaa caattcaaga ggatggaaaa aaagagagta aaaaatcaaa
    13921 tctagcaatt ctaaaaataa atgaagagca gctgaataaa aaatcacgaa tatttgatag
    13981 cgcccaatca gctgaaacct taaaaagtag caaggataca acctttgcta gtcctgaaac
    14041 gaaaaataaa cagctaccaa aatccggaga gtcacaaaac aaagttatac tgtggagcgg
    14101 aatcattcta ttaagtatag caacaatgct ttctgcaaaa agatttaaac aaaacagatc
    14161 tctctaacat ttgaataatg tctgtgaacg catttttcaa ataaaaagaa atatccagaa
    14221 aacgacccca aaataccaga ttgaaatctg atatttgggg tcgttcatac tttatctttc
    14281 ttcatattta ttttaaggga tatctgtttc ttttggaaga tggttcggga tctttttcac
    14341 tttcaataca tctaaaaaga aggtaaagat cggaatgccg acaatcaagc cccaaacgcc
    14401 aaagaaccgt tcgctaacaa gtaagaccac aaatgtataa aaaatcggca gttctgtttt
    14461 gctggacatc aacttcggat tcagcacata agactcaatt agatggacaa tcaaaatcgt
    14521 aatcaggata taaacaacat ctcggatgcc tccttgggaa taagctatga aagatagagg
    14581 aatacaagaa atgataacac ctgctactgg gatcagactt aaaatgaaaa tcatgatcgc
    14641 taaactcagc agctgatgaa agccaaatac agccaaggcg attgtagtga gcaacgtgtt
    14701 taaaagagcg ataacaaatt gagcttctaa taccaaacca aatgtattga caaacttatc
    14761 ggcaaaatag taaatatctt gaaagaacca agaaaattca ctttttaaaa acaatcttga
    14821 gaatacaatt gtttttttct tttcaatcat aaaaaagaaa cttaagatga atgacatagc
    14881 aaacgccact gccatttttc ctatatcctg taaatagcca agcgcaatcg aagcgccatt
    14941 ttgaagctgt tcaaaaaaat cgtttcgctc aatataagta tggatatatt gtaaaacagg
    15001 atctgcatct tttggctgat gctcgtaaaa atcaataacg gactggatca tatcaaatgt
    15061 ttgatgaata agcatcggga tatactttgt gaatgccaga tagataaggt aaacgatcag
    15121 tgtataaatc atgatagcta aaacacctgt agggattcgg acaaatcgct gaatgatttg
    15181 aacaagatga atcatcaaat aagtaaagat aaatgtgagc aaaatggttg taatcatact
    15241 tctaactaag aaaagaacaa aaacgacaaa taataaaaca gtgagacggc gtattttttc
    15301 attcgccaaa aaacgctgat aaacggtcaa atcgacaacc tccaaataac aaaaaataaa
    15361 cacgttattc attatatgat ggaatatgaa aataagaaag agaaagagca aaaagaaaaa
    15421 ccgctaaatg acgttatctt atgagttggt tttcctcata aaatcgttca tctagcagtt
    15481 tcagcccttt ttctggtctt gatgaaaatt atctagcatt tttcgggtca gatgttgcag
    15541 tgtatccaat tcttcctgtt caagtggaag ataagacttg atcttttgtg gaataccttc
    15601 tgcttgtttt tgcaacgcag caccttgagg agtcagcgta acatttacga ttctttcatc
    15661 acccgtttca cgctgacgat taatataatt tttttctgct aactttttta gtaagggagt
    15721 cagcgtacca gaatccagac acaaatcatt tcctagctct ttcactgaag tctttttctt
    15781 ttcccataaa actaacatca caagatactg ggtataggta agattcagcg gcttgaggag
    15841 ggaagtgtag tgtctaatga tttccttaga ggcggcatat aaaggaaaac atacttgatt
    15901 aactaaatat agatcttcca tttttccacc tccgctgttt tactatagtg tatcatagcg
    15961 gatttacaca aaaaagtcta ttcactaact gctttcagag taacttctat atttccagct
    16021 gtagcttttg aatacggaca cactttatgc gctgcctcta ataattcttg cgtttcttcc
    16081 agagaaagac cttcgataga tccttcaatc tctacaccta agacaaagct cgtttcactt
    16141 tctgaataaa gggaaacttg tgcactgaca gtagaagccc cagagatatt tttttgctgt
    16201 ttgatcaatt ctaatgcact gttaaaacat gcactgaaac cagctgcaaa caattgttca
    16261 gggttggttg cattttcgac gcgtttacca ggagctgcaa tattcaattc gaatgtacga
    16321 tcgggattat gaacttctcc agagcgtccg cctgtgttga tcattttcgt agaatacatt
    16381 tttttcataa aattaccacc tttaattata ttttatacaa ttgaatttta cacaatataa
    16441 ttgtggaaag caaagaaatg gattggcata agataaataa gagaaagcac atgaaacaga
    16501 cgcgctttct cgaatagcaa acatatacaa aaaaatatca gagacaaaaa ctaatctcat
    16561 cataaacatt acatatattt ctcaaaaagt tgttttcttt cttcaaaact tacttcacga
    16621 tggacctgat gaagcatcca taaataacca aaaggatctc tgaacattga gttagtcaca
    16681 ccaaagtcca tcatttctgt catttcttga atctctacac agccatttcg taatgcttta
    16741 ttatgtgtgt cctcgatatc agagacgatg atattgaacc agatcgtatt tggatgttca
    16801 gcagtaggag cagtcaaacc aagagacgtg ttttcatcca gcatatggaa tctcgtgcca
    16861 tacaaggtaa aaatgacttc attattgcct ctagggtaat ctgttgcctc aatcagttgt
    16921 atttcatcaa agattgtttg atacagttct agtgctttca aactatcatt taatacaaaa
    16981 tctatttccg ttcctttatg cataaagatc tcctctcctt ttcagtcgaa cagttactag
    17041 attcttttat tataccagtt aaagaaaacg cattcaaaaa aatagcggat ttacttacca
    17101 actcattcga ttggatgata agtaaatccg cgtcccatta cttctgctac gctgctgaca
    17161 atgacaaagg cacttggatc gatttcttct atcaatggtt tcattcggga aaattcatga
    17221 tcagtcacaa cgatcaataa aacctctttt gactttcgat tatacccgcc ttttgcctct
    17281 agcagggtaa ggccacgtcc aatctcagca cttaagcgaa ggcggatgtc ttccaaataa
    17341 tgttcactca tgaccataac agatttcttt cgattgatac ctgtttcgat ataagtcatg
    17401 acaattgaag tgattgcaat agataaaata gcataaagaa actcttctat tccaaaaaca
    17461 aacagattaa gagaaacaac gaatgcgtct gtaaaaaata agccaatcga agtattcaag
    17521 tggaaatatt ttttaaaaat gaggggaggg atcgtcgtac cgccgctaga agagttgttt
    17581 ttatataaaa tcgcaactcc taaagcaaaa atcgcacttc caaaaatgac agacaacaac
    17641 cgatctgaag taatcattgt ttctggaact attgcaatga ttaaaggaaa aaacaagctt
    17701 ccgaaaagta ctttaaaaaa gggtttttta cctaaaaata gaagagctag taccaacata
    17761 acgatattca acactaaaat aactatagct cggtcaaatc ctaaaacagc ttccagtaat
    17821 attcctagac cgctgacacc tccagcagca atatgatgag gccctaaaaa catattgata
    17881 gcgactccta atatgaagag ggcaaaaata atcttaatgt atgtccatat aaattgtctc
    17941 gtttctttca tttatccact tccttaattc attaataata ctcttatctt attataactt
    18001 acttttcacc ttaagttaac tttaattaat aaaaaaatta ggctagtcta aattattatg
    18061 tttcattctt ctagaatata cttctacgtt aaaaactgtt aaaaaacttt tactttagaa
    18121 aattggagaa attgatcgct taagataaat tcaggacact aaatagtaca ggaagtagat
    18181 taaaactttt aaaagtttta agtctatcta tttggcaagt ttaaaaaata aaaattcggt
    18241 atcaaaaaac tgttaccgat aacatttttg aaagcgttgt cttttgtttc tctatcactt
    18301 atagtgatga cgtcatatta aaacacaagg aggaaatcaa tggatacagc tgcaatttat
    18361 catcgaccgg aaagtgaata tgcatttcta tatacgaata caagatttcg tattcggctt
    18421 cgtactagga aaaatgatat caaaaaagta tatgtgcttt gtggagatcc atataccata
    18481 acaactgaaa aatggtatca aaaacaacga ccaatgaaaa aatgtctaag taccaatgtt
    18541 catgattatt gggaaattga agtgacgagc gaaacaaggc gcttacagta tgcgttccat
    18601 gttgttgggg aagataacac ggactgtttt tacggtgatc aaggaatttt tccttatcga
    18661 gaaaatgtga ttactgaacc aaatttttat tttcgaatac cttattttca tcaaatcgat
    18721 cgattcacta ttccagaatg ggtaaaagaa acagtatggt atcaaatttt tcccgaaaga
    18781 tttgcaaacg gggacaaagc aaatgacccc gaacacactt taccttgggg aagtaaagat
    18841 ccagaccgag aagacttctt cgggggagat ttgcaaggtg tgagggatca tttggattac
    18901 ttgacggacc ttggcgtcaa tggcatctat ttttgtccca tatttaaagc cacatccaat
    18961 cataaatacg atacgattga ttattatgag gtggatcccg cttttggcga taaaaaactt
    19021 ttaaaaaact tgattgatga agcacataaa agagggatac gtatcatgtt cgatgctgta
    19081 ttcaatcata tgggtgtcca ttcaccacag tggcaagacg ttctcaaaaa cggagaaaaa
    19141 tcgatctata aagactggtt ccacatccgt tttttcccag tagattctta tcagatgact
    19201 gacttaccag aaactgctga aaatatccca tatgatactt ttgcttttac tccattcatg
    19261 ccgaaactaa atacagcaaa tccagaagtg caaaaatatt tgctggatat cgcaacttat
    19321 tggataaaag aattcgatat tgatggatgg cgtcttgatg ttgctaatga agtcgatcat
    19381 catttttgga aaaaattcag agaagcagtt acagaaatca aaccagacat ctatatcctt
    19441 ggagagattt ggcattcctc tcaagcttgg ctgcaagggg atgaattcca tgcagtgatg
    19501 aattatgcat tcacagattc gattaaagat tattttgcaa aaaagaaaat cacagcttca
    19561 caaatggtca gtggaatgaa tcatcagcaa atgctttaca gagatcaggt gaacgaagga
    19621 acattcaact tattagattc tcatgatact gccaggatct tgaccctttg ccaaggtaac
    19681 aaagaattga tgaagtctgt gatggccttt atgtttcttc aaaaagggtc tccttgtatc
    19741 tattacggga cagaaatagg gatgactggt gaagatgacc cggattgccg caaatgtatg
    19801 atatgggaga aggaggaaca agatctagag ctattcggtt ttataaaaga acttgtttct
    19861 ctaaggaaac agttgtctaa gattatttct gaaggttcta ctcagtggct aatagtatat
    19921 gatcgtgaag ataagctcta tttcacacgg gaactagagg gacagatcat ctatgtttac
    19981 tttaatcagt caaaagaacc ttgggtagta gaacaggaaa atgaggtgat tcttagtcaa
    20041 aattgtcagt tgttagaaga cgggaaagca gagatacgac aatacggatt tttgatcatg
    20101 tgttgaaaac tgttaccggt aacaacaaga aagcgtttgc aaatgaagat ttgtgcccat
    20161 tataataagg gtgtaacagc acgttgaata cacaggagga aatgaagatg aaaaaaagaa
    20221 gtgtgaaaca gttgggttta gggttaatct ctttaggaat cttaggcgga ttagcggctt
    20281 gtggcaatgg taactcttca gcagaagggg aagccgatga aggaaaaacg ttaacagttt
    20341 ccgttgattc tggatataaa gattatataa acgaaataaa aaacactttc gaaaaagaaa
    20401 acgatgtgaa aatcaagcta gtagaaaagg acatgttcga tcagttggaa tcattagcgt
    20461 tagatgggcc tgctggaaag ggacctgacg ttatgatggc agcatatgac cggatcggtg
    20521 cgttaggaca gcaaggacat ctggcggaag taaaactggg aaatgaagca gcttacgatg
    20581 aaacagacaa agcccaagtg acttatgatg gaaaaatcta cggagaacct gcagtaatcg
    20641 agacccttgt tttgtactat aacaaagatt tagtcgatac agcaccatca acgtttaaag
    20701 atttggagaa tctatccaaa gacagtcgtt ttgcttttga gtcagaagca ggaaaaaata
    20761 caggattttt agctaaatgg actgactttt attattccta tggtttaatt gcaggttacg
    20821 gtggctatgt atttggtgat gatggcacaa atccttcaga tatcggtttg aataatgcag
    20881 gtgctgtaga aggaatctct tatgcaacag attggttcaa aaatgtttgg ccaaagggaa
    20941 tgcaagacat aaaaagtgca ggagacttcg ccagccagca attcatgtcc aataaaactg
    21001 cagcaatcat tgacggtcca tggcaagcgc aaacatacaa agaaaacaat atcaattatg
    21061 gtgtagccaa aattcctact ctaaataatg gacagcctta tcaaccgttt ggcggaggaa
    21121 aaggatgggt agtcagcaat tatgctaaaa acaaagacct ctctcaaaaa tggcttgatt
    21181 atgtaacgaa tcaggaaaat caagaaaaat tctttgaaat ggtaaatgaa atccctgcaa
    21241 accaacaagc tagggaaaca gcaaaggcta aaaacgatga attaacaaca gctgtgattg
    21301 aacaatatga aacagctcaa gcgatgccaa acatcccaga aatgggcgaa gtttggacag
    21361 gtgcagaaaa cttgatgttc gatgcagcat caggttcaaa aacaccaaaa gaatccgcag
    21421 atgaagcagt aaaaacaatc agtgaagcca tagaacaaaa atactgatta aaatgataac
    21481 cgtccatttt tataaaaaat ggaacaggtg cttcagataa aagtggcaag agcgagatgg
    21541 aaaattatct tgctcttgtt gccattatct taaaagcagt ttgcggttta gcaagtttag
    21601 agggggcatt cagatgcagc aagataaaac aagcaatatc aacgaaaaaa aaatcacgct
    21661 ttgttcactt attccaggga tggggcagtt tgtaaatggc caagctttta aaggtatcgt
    21721 ttttttcgcc atattcgtcc tattcattgg tcagatgctt ctgggcggat ttcatgcatt
    21781 ggtaggattg gttactttgg gaagtatacc aatggaagat cattcattat ttattttgat
    21841 tgaaggcact ttgcaattca tcattacaat cattttcttg attttttacg gtttgaatct
    21901 tttagacgct tatcgagtag cgaagatgcg tcaagagggc aaaaaaatca atcggacgat
    21961 gaaggaagtc atacataata taggtgatga aggttttcct tatctattga cacttcccgc
    22021 ttatcttctc atgattttta cgatcatttt cccagtccta gttacattgt tcactgcatt
    22081 tactaattat gacttcaatc atattccacc agccagttta attgattggg taggggctaa
    22141 aaactttttt agtatcttct ttttaagctc atatcgtaat actttttttg cagtcttttc
    22201 gtggaccgtg atttggactt tatgtgcaac aactttgcaa atcacccttg gtattctcac
    22261 agcggttgtt gctcaccaga aatttattaa attcaaacgt attttcggta tcattttctt
    22321 attaccatgg gcggtcccag cttttattac tatcatgagt ttttcgaata tattcaacga
    22381 tagcgtaggt gccatcaata ctcaagtgat tcctttcata aaccagcttc cttttgtaga
    22441 tatcagtgca atctcttgga aaacggaccc gaactggacg aaagtagcaa ttatcttagt
    22501 gcagggctgg ttaggatttc cctatatcta tgtgatggtt acaggaatct tacaatcgat
    22561 ttcagaagac ttatatgaag cagcaaaaat cgacggagca aatgcatggc agcgcttttc
    22621 aaacatcacg cttcctgctg tcttcctagt ggcagcgcca acatttatta cacagtatac
    22681 ggggaatttc aataatttct ccatgatcta tctgtttaac aatggtggtc ccggaagcgt
    22741 aggcggagga gctggttcta ctgatattct tatttcctgg atctataaac ttacgactgg
    22801 aacttctccg cagtattcta tggcttcagc aatcacgttg attatctccg tcatcgttat
    22861 ctgtgtgtca ttgctggtat ttaaaaagac aaatgcgttc aatatggggg attaacaatg
    22921 aaaaaatttc attcgagtgc tcgattccaa aaacgacttt ctcacttttt tacttacttt
    22981 tttcttttaa tactgtcagt cattatcatc tatccgcttt tgatcacagc aagtactgcc
    23041 ttcaagtcag gaaatattgc agcttttagt ttagatcttt caagtgactg gactttagga
    23101 aactttactc ggctattcag tgaaacactt tacgggactt ggtataaaaa tacattgatc
    23161 atttctgtcc taacaatgat cgtacaagtg actgtagtaa ctttggcggg ctatacttat
    23221 agtcgctatc gttttatcgg ccgcaaatca agtttgatgt tctttttagt gattcagatg
    23281 gtaccgacta tggcagcatt gactgccttt tatgtcatgg ctttcttatt agaagcgctc
    23341 gataagtact ggttcttgac aatgatctat ataggcggtg gaattccaat gaatacttgg
    23401 ctgatgaaag gatactttga cactgtacca atagatttag atgaatctgc taaacttgat
    23461 ggggcagggc atttcaggat ttttttccag attgtattac cgcttgtcaa accgatgctc
    23521 gccgttcaag cattatgggc ttttatggga ccttttgggg atttcatgtt agctaagttc
    23581 ctgcttcgaa cacctgaaaa tcagactgtt gctgtcggct tacaaacatt tatttctgat
    23641 gtgacaaacc aaagagtctc tctttttgct gctggggcaa ttttgattgc actaccgatt
    23701 tgtctattat tcttcttttt acaaaagaac tttgttaccg gcctgacatc tggcggaaca
    23761 aaaggatagg agcagagtat catgcaaaca tttccgttaa actatttttc cgcattacgt
    23821 acgccaactc aagtgtttgc gggacgcaaa ctattatcat ggcctaaatt tttcttgata
    23881 tttgtatttt tagtttcctt gatggtcatg cctgtgactc ttttttatgc caatcagatc
    23941 caagcgattc cattagaaca atttctatcc gttcattcgc tgatcgatga acaaggaacc
    24001 caaaaatttt ctgaacttga attatctgaa actgggttac aagcatcgca acaaacgata
    24061 gctgtgaccc ctgaaatttt ggtaggtgtg tctttatctg agaaacaaca atccgatcat
    24121 ggaactttta ttgattttga gaaggaacaa tgggtgatcc aacaaaagga taaatctggt
    24181 atacgccgct atacgatgaa ttatagccca tctttccaac cagattctgt acgtacgccc
    24241 gaagatttcc aacgattttt agaaagagaa ttttatgcca gtaatcgtcc aacaatcatt
    24301 ttatcttata gtttaagtct tggtcttgtc ctatttgtca tgacgagtct cattttattc
    24361 ggggcttcct ttttcttgtg gatgacaaga aaaagccaac tttcatccat tcatacattc
    24421 aaagagagtg caaatttgat gctgaatgtt atgggaatag gttccatggc agcagcagtt
    24481 gtagggttta tccactttga ctttatcttg atgctaagtg tccaaacaac gataactgtc
    24541 ttgctgcttt tatgggtttt tgtcaaaaca agattcaaag ataagcgtgt cgaatgacaa
    24601 gtttaagcga tttatgcgac aatgaatgtg aaaaaaatga aaaataaagt agtttttgaa
    24661 aaatttctga aaaaattaaa cttcttagtt gattcttttc ttttttagta ttaacgtaat
    24721 agggtagttt ttttaaggag gaaaatcatg ttagagaaaa ctttatacaa tcaattattt
    24781 tcacgttctt tctcgctccc tgtcgaagta aattattggg acggtacaac gaaacaatac
    24841 ggagatacgg acactcctcc ccaaatcaaa atcaatatcc atgaagaaat cccaatgaaa
    24901 gagattacaa gtaacgcttc tttggcacta ggagaagcct atatggatca tcgaatcgag
    24961 atcgagggaa gtatccaagc attgatcaac gatgcttatc agcaagcaaa cagcttttta
    25021 cgaggaaaag attatctaaa atggctgcca aagaaagaaa aacatacgaa acaaaaaaac
    25081 aaagaagata tccatgcgca ttatgactta ggtaacgact tttataaact ttggctagat
    25141 ccaactttga cttattcttg tgcttatttc aatacacctg aagatacgtt ggaacaagca
    25201 caaatcaata aagtccatca tatcttagac aaactcttta tcaaagaagg cgaaacgttg
    25261 cttgatatcg gttgtggctg gggaacattg atgttcactg ctaccaaaga gtataatgtc
    25321 aaggcaactg ggatcacact aagtgaagaa caatacgatt tgattaccaa aagaataaaa
    25381 gaagaacatt tagaagataa atgccgtgta ttattaatgg attaccgcga actaaaaggc
    25441 gaaacgtttg atcatatcac aagtgtaggt atgttcgaac atgtaggttc tgaaaatctt
    25501 gaaggttatt tcaaagtggt aaaagattta ttgaaaccaa aaggaacagc tttgatccat
    25561 ggaatcagcc gacaacaagg cggtgcgaca aatgcttgga tcaataaata tattttccct
    25621 ggcggttatg tccctggtgt tactgaatta gttgataata ttacaaaaaa cgatttgcaa
    25681 ctgattgatt tggaaagctt acgtcgtgac taccaattga cattagaaca ttggacaaag
    25741 aattttcatc aagtccaaga tcaagtcacg aaagaaatgg gtgattcctt ctatcgaatg
    25801 tgggatctat atttacaagc ttgtgcagct tcatttgaat caagtaatat cgatgtgatt
    25861 caatatttgc ttgttcaacc tggaaacaat gacatcccaa tgcatcgtgc ataattgagg
    25921 caaaagtact gtttaaagac tatttaaaaa gaagctaagt tttatgatgc tccagaagtg
    25981 aacttttttg tctaacttct aggattgtct ctaaagctta gtttcttttt ctttatattt
    26041 tcataataaa agtttggttc tttaattttt aaacatgaca gaaaattttt tcttcgttat
    26101 acttgtaata gaatactatt ttataacaaa ggggatcgaa tcaatgaaaa aaactgttat
    26161 ttatactagt ttatttgcag ctttgttttt agctgcttgt caatcaaata ccaatcaagg
    26221 cacatctggg aatacgtctt ccagtagcga acagcgctct tcacaggtat cggactcctc
    26281 aacaaaagca agcacatctt cgtctagcca gacaaaaagt tctagtgaag caagtactca
    26341 gtcagatatc aaagtgtctg cagctcgtgc gatcgaattg ttccaagata aatacccaga
    26401 tactgcaatt actagtttgg aattagatag tgattgggga tcttacttct acaagataga
    26461 aggtgtcgat gatcaaaaag aatacgaagc aagaattgat gctcaaaatg aaaaaatgga
    26521 gacagagaat ccagaaactc tagatcgaga tgaacaaaat ggtcaaaaga aaaaagaaga
    26581 tggtctggat gttgataaat tgatcacgat tgaggaagca gcaaaaatcg ccgtcgacaa
    26641 agtaggaagc ggtacggcaa cagattggga tttggataaa gatttaggta ctacttattg
    26701 ggaagtcaaa gtaaaagacg gcaatcaaag tacatcagtc aagatcaata gccaaacagg
    26761 cgaagtactg tcaactgaac aagatgatta agaatttctt cggaaaacaa gagttgattt
    26821 ttcttttagt catgatacac tagacaaaat aacttgtttg aaggagcgat ttacttgagt
    26881 ttagaaacga cctttgccaa tcatatcttt gccaatcctt tgatgaatgc atctggtgtc
    26941 cactgcatga ctacccagga attagatgaa ctggctcatt cagaagcagg agcatttatc
    27001 actaagagct gtacgatcaa tgaacgaaaa ggaaatcccg aaccacgcta ttttgatgtt
    27061 cctttaggga gtatcaattc tatgggactt ccaaacttag gtttttctta ttatctagag
    27121 tatgctcttg cgtatgaaaa agtgcaggaa aaccaaaatc aaccgttatt tttttccatt
    27181 gccgggatga gtgtccagga aaacttggaa atgcttgaga aaatagaaaa aagtgggttt
    27241 aatgggatca ctgaattaaa tttatcttgt cccaatgtgc ctggaaaacc acaactagct
    27301 tacgattttg aggccactta tgaaacactg aaagaagtat tcagcatctt ttctaagccg
    27361 ctaggaatca aattgcctcc ttattttgat tttgctcatt ttgatcagat ggctgatatc
    27421 ttgaatcagt ttccattgac ctatgtcaat gctatcaaca gtgtgggcaa tggtttgtat
    27481 atcgatacag aacaagaagc agtagtcatc aaaccaaaag aagggttcgg cggcattggc
    27541 ggagagtata tcaagccgac tgctttggcg aatgttcgcg ctttttatac acgtttgaaa
    27601 ccagaaatcc aaatcattgg tacaggtggg attcggacag gacaagatgc atttgaacat
    27661 ctgctatgtg gcgcaagcat gttacagatt ggtacagagc ttcataaaga agggccggaa
    27721 attttttcta gaatcataaa agaattaacg cagattatgt cagaaaaagg atatacgtca
    27781 attgacgaat ttaaaggcaa attacgcacc atttcatagc aaagagacga aggtcatgat
    27841 tgatcttcgt ctttttttat aaaaaacact cgtctgttca ttcctgtgca agcgtactca
    27901 ttgtatgtta gacgaacttg tgggaaaata acagaggcaa aaaggaggat aatatgaatg
    27961 atgcaacaga attagcaaaa gagcttaaag aaaaagaatt tagttttcaa gaactagtta
    28021 cggatataca gaaaaaaata aagaagcaaa atcatgaatt gaatgcgttt gtcgcgcttg
    28081 aaccattcga gagtaattcc cacgagctat tgattcccca gtcagaaaaa gggccattgg
    28141 ctggtatccc atttccttta aaaatgctcg gtcaagaaaa aaagggatgg ctagctactt
    28201 caggatctcg tttatttgaa acacaccgag cttctcatac atctaattat gtgcggcaag
    28261 cagaagcgat aggtctagtt ccttttggac aaaccaatgc gccagagttt ggtttcaaaa
    28321 atattacaga cccagtcatc tatggcccag caagaaaccc ttggaacctt gaatgtacac
    28381 cgggcggatc aagtggagga gctgctgctg cagtttcatc aggaatcgtt cctttagctg
    28441 gtgctagtga tggcggtggt tctattagaa tacccgcttc gttttgcgga ttgattgggc
    28501 tgaaaccttc gcgagggacg atgccagttg gtccatatgc ttggagagga tggcaaggag
    28561 ctgctattga tttcggcttg acagtttcta tgagagacac ggaggcttta ttttatggga
    28621 tgagaagtat ccattccggt gcgccatatc aagtatcgcc agttgagtgg caaacacatc
    28681 caagaaaaaa acggttgaag atagcttttt gtgttgattc accgatccac tcacagatta
    28741 gtgaagaggc agtacttgct gtaaaaaaag ctgtagattt ccttggaaaa gaagggcatg
    28801 atttgacaga gattccttac ccactagatg gtcgtaaact gatcacgtct tattatcaga
    28861 tgaatggcgc agaaacagca gcaatgatcc gctcgattga aacaggcatc cagcgagctg
    28921 tcagaaaaga agaacttgaa cccatttctt ggactttgtt ccaatatggt cagaagctct
    28981 ctgccgctac ttacatcgat tctctgcatg tatgggatga agcagcaatt gtcatggaaa
    29041 acctacatca agaatacgat ctattccttt caccaacgac tgcatttacc gcccctaaaa
    29101 tcagcgaaga tttgcaaagt gatgcgataa gagcacagat ggaacacatc agtgattgtt
    29161 ctgaagtaga aggtttagac gttgtttctt ccatgtttga ggagagcttg aagatcacac
    29221 cttatacaca gttggctaat ctgactggac aaccagcaat cagcttgcct actcatttga
    29281 caaaagaaaa catgcctcta ggaatccaat ttatggctgc cagaggaaga gaagatttgc
    29341 tatttcaaat cggctatcta tttgaagcaa atcatcagtt ccatttacca aatccatgga
    29401 aatagcagaa acaaggagaa gaagaatgaa gaaaaaaaga ttgtttgctc taataactag
    29461 tgcctttttt attttaagcg cttgcggtac agctgccgct aatcaggaag ataacagctg
    29521 gagtaatgta gaaaaaaaga aagaattagt agtagcgact tctggcacac tgtttcccgc
    29581 atcttattat aatgatcaaa atgaattagt tggttatgat gtagatttgg caaaagaggt
    29641 ggctaaacgg ttaaatgtca aaatctcctt taaagaatac aacgtggatg gacaaatcac
    29701 ttctgtttca aaaagagaat cagattttgc tgcgaacgat tttggtatca caaaagaaag
    29761 agaaaaaaaa ttctcccttt cagagacttt caaatattca tttgacagta tgatcgtacg
    29821 aaaaaaagat gattcaggaa tccactcact tgaagatctt aaagggaaaa aagcagctgg
    29881 cgaacccaat actagctata tgcgtttagc ggaaaaacac ggagctaaat tagtcacata
    29941 tgacaatgcc acgaatgatc agtatttaag cgatgtagca aatggtcgaa cagatgttat
    30001 tctaaatgat tattatctgc agaaaatggc cgtatctgct ttaccagata tccctgtaaa
    30061 aatactggag aacgtttatt tcaatgaaaa cgaaagtggt tttttattga aagaaggaaa
    30121 tgatgctctg aaaaagaaaa tcgatggcgt attaaaagag atgaaagaag atgggacgac
    30181 aaagaaaata tctgaaaaat attttcaaac tgatgtttca gtcaaaccga aagaaacgat
    30241 ggctgagtcg gtgagtgagt gatgtacttt cccctaattg atttttccct catgtggact
    30301 tcacttcctt tcgtactgca aggcttggta tacacattag ggattggctt cgtgtcgttt
    30361 cttttaggaa atttggtagg gttgcttttg acagttttag gtcttttgga ttggctgcct
    30421 ctgaatgtat ttattcgttt ttatttatcg ttctttagag gcattcccgc actagtcctg
    30481 ctgtttctgt tatatttcgg gttaccttat caattaagtg ccttaacggc ttcagtcatc
    30541 tgctttacga ttaccagcag tgcatttatt ggtgaaatct atcgaggatc actagcaggc
    30601 gtttcttcag gtcagtggga cgctggttta gcttctggtt tttctttctt tggggtattg
    30661 aaatatatcg ttttgccgca agcttttcga atctcagtac cggcactcag taacgtagct
    30721 atggatctag tgaaaggtac ttcgttagca gcaacaatca cagtgccaga aatatttcaa
    30781 aaagcaaaga tcataggcgg tcggacattc gattattttt ctctctatat tttagttgca
    30841 ctgatttatt gggtcatttg tatcgggatc gaaaaagtgc aacagtattt agaaaaacgt
    30901 tctatccatc agtcaatctg aaaaatagaa atggtgctag cgtggctggc accatttcta
    30961 ttttttgttt cttgtatatt ctgcctagta ttttaaaacc atttcatgta aaatgtaaat
    31021 cgaaaatatg ttcggaggta gaaaacatga agaccccgct aaccatcgaa atagaatcct
    31081 cgttatacca atattgcatc gagcaaggcg gcttggttgt agaagaagtc acgatgcctg
    31141 atgaccaagg aattgtagat acgcttagct gcttgacaaa atttgatgga agccgagaat
    31201 ggcgatgcta tgagctgaaa gtgacaaaag cagattttcg ctctacagcc aaactgtcat
    31261 ttgttggaca ttataattat tttgttttac caagtaaact atatgaagaa gtaaaaactg
    31321 aaatccctac aggaataggt gttttagtgt atcgtccgta cacggttgaa gaggagatgc
    31381 ctgcctctgg tacatttctg attgcgaaaa aagcagcaaa gaaagagctt gctgtaccgg
    31441 aagaagcttt gacaaaccgc ttcatggctt ctttattcag ggaagtaaga aaagcaaagc
    31501 aaatggagcg aggcgtaaaa tattttccaa aagatcaatt gtacaaagaa ttgaaaagac
    31561 gttcagaaaa tcaagatcca ttttctaacg aacgatatta tgaacggttt ttagaggaaa
    31621 tagctagtca ggcaatcaat gagttgcaag aagaagttat cgcgcttcag catgattatg
    31681 aatatttgag gcaacaacag cagatccgtc ggttgcctac agagccattg gagtgaaaaa
    31741 tatgtactat ccttatttaa gaggaaaaca atttgaccta ttagcattaa aagaagcact
    31801 gtcgcgagga ttgctctcca ataaaatcca accagtgatc gaaccagtac gtgattccgc
    31861 tacgcttaaa aatgtgatcg aattatttca aaaaaagcac catccaatcg ctgtgatcca
    31921 aaatccccaa gtaggacaat tcaaattatt cgatcagcat gtgcacagtt ggacagtcaa
    31981 agaaaatagc tctgtcgtcc ctgcacagat cttaacgcca gaaaatctca gtgaagtact
    32041 ggattcacct ccagcgtttc ttgttttcga tggacagcac tacccaaaag atacagaggt
    32101 gtggaaacag ttggcaggag ttgattcgaa atttttgata ccagatacta gtcgctttcg
    32161 catctggctg cccgaaaaca aaattgtgat ccgtgactct tttcaaacga gaaagcatgt
    32221 tgaaagttat gcagataaaa cagatgactt ttttagtgat gactatcttt ttttccacgc
    32281 agacggatat attggatttt ctgatttcac gatcgaaggt agccgttatt tcgataaagg
    32341 gttccctagc cgagcgcttg ctatccatct aacgtatatt gatgcttacg gcaatatacg
    32401 cgtgaaacat tttgtctctg acagtaacga ctctgctaaa gaccaagcat tgaagtttct
    32461 tgaagcagga gataaaatga aaaaatggat catgcgtcac catcatcaac tcttgattac
    32521 ttcaggaatg attgaattat tggctcttta ccagcaacaa aaattccctg gactgggagt
    32581 gttgaaaaag tggtcactta tgcatcattt ggaacttatt agtcacctgc tagatcatcc
    32641 aaaagattgg cttaaatatt attcaaaagt tcaggaacta tatgaagtga tccaatgatt
    32701 agataatgta aatgaaaaag gaatagaaaa gaggaaactg gatgtatatt cggcaaacaa
    32761 ggaaagaaga agtagaagaa gtggtagaaa tcattaatgc cgcagcggat ttcattgctt
    32821 cgcaaggctc tcctcaatgg caaaacgggc agaaaccaac aaaagaaacg attttgaacg
    32881 atattgaaag aaaagaaagc tatgtattaa tagtggaaga tcaaattgct ggaacagctg
    32941 cattagtctc aggcgtggat cccgtttaca caaaaataga tggtagttgg aaagaatcaa
    33001 ctggtcctta tttatcgatt catcgtgtcg ctttagcaga aagtactcga ggaaaaaaac
    33061 tagcaaaacc gttcttagaa catttgttga cgattgcagc cagcaaaaat atccaagata
    33121 tccgtatcga tacttatcca gctaatgaac cgatggaaaa gaccatctat tctgctggat
    33181 tcacttatca agggatgatt gaatttcctt ttgaacacgg cgaaagaaaa gcatatcaaa
    33241 taatcatcaa ttgactataa aatcatctga gcaaagataa agacattaac actactgaaa
    33301 aaaacaatca tatgatagcc aagaggttgt aacagaagtg gccagcttca aaaaacaaga
    33361 aagaatttcc gaaaatgctt ctcaaatttt tgtggaattt tagcgtattt tacgaaatag
    33421 ctgctttctg aaactccctt catcgagagg tcgtgacata gcttttgtca cgatctctcg
    33481 gcttatccta cgagtattat tcgatacatt tatatcaata atcctttttt tattttatct
    33541 gcagcttctt ggaacgctcc atcgtttgac acgactaaaa gtgaggggat agctttcaaa
    33601 cgtttcaact gtgctaaatc ttctacataa accgccagtc tggctgaaat ctgttcagat
    33661 gaatctccac gttttttcag gcgcgattgc aatgtttctt tagagatcgt aataaagata
    33721 ggacggacta ccgtcccgaa atactcatat aaatgccaaa aaccatttgc tgtcaaaacg
    33781 gcgtaacaat ttcctactgt cagtttcttt ttaatttctg ctttactgga accatattta
    33841 tgttgagcat attggtccca ctctgcaaac tcatttccct tgatcatctg attgaatgta
    33901 ttgtcagaaa caaaataata atcctcttga tctttttccc caggtcttgg tgatcgattc
    33961 gtgtaagtaa tgatcttttg ttccggctga aaaatagctt ctgcgagtga agtttttcca
    34021 gaaccagaag gaccgataaa tacatagcaa taattcattg tcatttctcc tttgatgatt
    34081 gaacaagtga aaaagctttg gaaaatattt ccaaagcttt taaaagtaac tttgcttttt
    34141 tgtattttat accagcaagc cagtcacaat agcacttaaa aagctgacta aagtcgctgt
    34201 tagcaaaatt ttcaatccgt gttttgcgac aagatttcct ttttcttcat ttaatccctt
    34261 catcgcacca gaaatgatcc cgatagaaga gaagttggca aatgaaacga ggaagacaga
    34321 tataattgct gttgtacgct cagtaaaatg gaaattacca tttgctaatt ctgtcatagc
    34381 aacaaattca tttgtaacca gtttagtcgc catgatacta ccggcatcca ctgcttcttt
    34441 ccatgggatt ccgctgatga aggcaagagg ggcaaagatg taacctagga tctcttggaa
    34501 agtgatgccg aatatcccgt ggaagatagc attgatcatg gcaatcaaag caacaaaacc
    34561 gataagcata gcagcaaccg tgatcgccac atggaaacca tcaagaatgt attctcccaa
    34621 catttggaaa aatgtctgtt ctttgttttc ttcaatgatc agctcatctt ctttttcttc
    34681 taatacatag ggatttacaa ttgaagccag aatataaccg ccaaataagt tcaaaacaag
    34741 ggcagtgatc acatatttag aatcgatcag tgccatataa gatccgacga tcgacataga
    34801 aactgtagac atagccgata cactcatggt gaacaaacgt ttctcagata ggaagggtaa
    34861 ttcctttttt atggaaataa aaacttccga ttgtcctaaa atagcagaag ctactccatt
    34921 ataagattcc aatttgccca tcccgttgat tttacttaat cctagaccaa cagctttcat
    34981 aaataatgga agaatcttga tatagcgtag aataccgata atagcagaaa taaatacaat
    35041 tggcatcaat acggaaagaa agaaaggtgc acttccttta tttgctacac cggcaaaaac
    35101 aaagtcaaca ccttgcccag caaaagcaag aagatgatca aacatttttg ccaatacaga
    35161 aacaaccgct gtaccaaaag ttgtacgtaa taatacgaaa ccaagaacaa gctgtaaaac
    35221 gaacatcaca attagtggtt tcaatttgat tgcttttttg ttactgctga tcaaaaaagc
    35281 aagccctaat gtgacaaata aaccaataat acctaataaa taactcaaat aatcactcct
    35341 attccaaatt ccgttttatt ttacctgttt tgcaaataaa atactaatct ttagtgaaat
    35401 tttcattact tttttagagt gaaacgcatt cattttccat tagaaaataa agcgaacaaa
    35461 ataaaaggtt taaaaagtgt aacgtcttag taaagtctat aaaaaaacga aactatcgat
    35521 taagctaata gtttcgtttt caacatttat aagtaaactt tttctattgg taatatagat
    35581 tttcggaagg gtagacaggt gcgcaggtaa tatcgattcc tagttttttc aatgtttgtt
    35641 catcattttt attgaccatc acagtagagt gggcttgtgc tccctcaagt tcagctagtt
    35701 tatcataagc aacttgagcg attggattcg ttactgcact gattgataaa gcgatcaaga
    35761 cttcattgat gcttagtgca tccaaagagg aatgaagtgt ctttgatttc aatccttgta
    35821 ttgtttcaag aactaatggt gacaataaag gaatgtcatc tgcgatatgg gctaaatgtt
    35881 ttactgcatt taagataact gccgctgacg catccatcaa tgaacttgta cgcccggtaa
    35941 tgatgacttc atctggtaat tcgattgcaa tcacagcaga aggatctttt gtaggacttt
    36001 gcgcttgaat cttatctgca tagtcatggg cagggactac cacgtttcgg tcttcaggac
    36061 gtaaaccgac ttcttccata atcagtttga ttcgattaac tgcttcttcg tcgatcaatc
    36121 cttttttgaa gtcgcattct gtttggaaat aacgacggat gatttcttgt ttagaggctt
    36181 cttggacaac ggtatcatcc acgatcccaa atcctacacg attcacaccc atgtcagtag
    36241 gagattgata aacagattct ttaccagtaa ttttttcaat aatacgtttg attacaggaa
    36301 atgtctcaac atcccgatta tagttcacag ctactttatt gtaggcatca aagtggaagg
    36361 aatcgatcat attgacatct tttagatcga ctgtggctgc ttcataagca atgttcagcg
    36421 gatgtttcaa cggaacattc catactggga aagtttcgaa cttggagtaa ccagctgcat
    36481 gtcctttttg gctttcatga tacagttggt tcaagcaagt agcgagcttt ccacttcctg
    36541 ggccaggagc tgtcactaca acgattggtt tagttgtttc aatatactca ttttttccaa
    36601 aaccatcttc tgagacgatt ttttctacgt tgactggata gtcttcgatt tctgagtgtt
    36661 tatagacctt gatgtttcgt ctttctagtt tattgataaa gactttcgtg gcaggctgtc
    36721 cactatagcg agtgataacg acactgttga ttaataagcc ataaccacgt aattcatcga
    36781 ttaaccgcaa gatatccata tcataagtaa tcccataatc accacggatc ttattacgtt
    36841 caatatctcc agcatagacg caaatcagta tttcagcttg atctttcaat ttttgcagca
    36901 atttgatttt cgcatcttct tcaaatcctg gaagtacacg tttcgcatgc ttgtcgtcaa
    36961 ctaacttccc accaaattct aaatataatt tgtcatagtg gttcactcgt tccaaaatat
    37021 acgctgactg ctcttcaata tatttttctg aatcaaaacc aatttttttc actaggttcc
    37081 ctccttaagt actaaagcat tttttcattt ttttgaggat tcgtcaactg gaagagcgaa
    37141 attcctcaaa aaaacaaaga aaatggttta aaccaacttg tctagatagt tattcattgg
    37201 tatgatagtt tttgataagg aaggtgctac atgaaaaaaa gacagaaaaa gaaaaacgca
    37261 tataaacact atatccgttc gatttttacc ggttatgaaa gaatgcttga agatcccgaa
    37321 ttggaacaat tgacgtttac ttatctaaac gaagaaacgc agctaactcg tgatgagcat
    37381 cagcgcattc atttcacaac aagagatctg cctagtaaat aaaaaataag ataaaggtca
    37441 tcggattcga ttctgtccta tcagatggaa agactagttg atcgaaaaaa aggctgtacc
    37501 aaattttggt gcagcctgtt ttatggagca ttgcccgctc ttttttataa caatttttta
    37561 cagaaaatgg tactattgaa gtattaataa gtgaaaaaca gatgagtaag aggaggcaag
    37621 tagtatgaaa gagccagata caaatgcaaa ttatgagcca gacgaactag aggagacgaa
    37681 cgcggatcag gatacaggac aagaatcgtc ttctttttac gatatcaaag aaaaagcaag
    37741 catgaatctt tcccaattat tagaagactt acaggctttt gaaaaagctg tccgcgaaga
    37801 agatatccct gagatctacc gtttatacaa tggccgattg aatgaagaat taaaaagaag
    37861 ctcaaatgaa aatcacgaga tcgatcaatt gctgatgcgt aagatccatg atcgtttttt
    37921 acaggaattt ccttttatgg aacaagtaga aaacatctcg ccaacgatga gctattacaa
    37981 aatcggcact tattaccatg atcggcccac ggtcggtatt gatgcaagtc tgccagaaat
    38041 ttttgttttg cctaagatcg atgaagagtg ggagaaatat tcccaaccgg agtcaacgga
    38101 ctatgacaaa aaaatcaatg agctagacgc aaaggtgatc actgctcaga cagaaattga
    38161 acgtttagat gaacaaataa aagaaatcaa tcgacaaatg actgatttga acgacaataa
    38221 aggattcttg aatcgtaaaa aaatcgagga agaaattcaa gaattagaga agaagaagca
    38281 agtactatct aatgaaaaat taggctggct tccttatatt gagacgcctg aaacaatcca
    38341 gcagcaaaaa gaagcattga agcaagaagc aagagcagac cagcttcgtg cagccatcgt
    38401 agaaaaagaa caaaggcaaa tcagacgcta tttcggtagc aaagagggat tcggtcaagc
    38461 aatccatgaa ttcttgatga attatcttgg caatgagaat cctaataatc aatctaatga
    38521 aggaggttcc gagtatgaat gaacacgatg aagcagaatg gacaaaaaat gaagaacaaa
    38581 cagaagatca tgaagaaacg aacacttcat tggaagaaca aacaagtaat cctctggatg
    38641 acacagaaga aatccctatg ctagaggaaa caaaaataca tgaaaaagaa aaagtaactc
    38701 cgacgaatac gaatactgca gaaaacaccc atgcttacga aataactgaa aatcccgggt
    38761 atttagaagg aaaagagcaa gatcatcaag atatcgtcag tttgattgaa acagcggaag
    38821 cagaattagc ggatttacgt atgagaaaag atttgatcga atcagatttt ccacaattat
    38881 tagaatcata tgatcgtttt atattgaatg aagaaccaat ctccacgatc ggaaagacaa
    38941 gcctgatcga atacttcacg tatgaattac tgaaaccgtt gaatgatatt ggtctggaac
    39001 ggtcaaattc agcttatgac acatggaaaa caagacattt ttcgatcaat tatacgttga
    39061 atggacaaaa cgaattagtc ttttcctttg tattaccgac catcgatcaa cgataccaag
    39121 tggaatcgat gagtctccta aacgtatcac cagagacaat ggaaatcaat gtccaagatg
    39181 accgggtgct ttctttgatc cgacactggt cagtggatcg agtattctct gcagggcaga
    39241 tcacgatatt caatcacaaa ttgaatcaat tattagccca tgcaagaaca cttggtttta
    39301 ccgttaatca gacattacta gacaatacga agccacttcg tttgaatctt cagagcgaat
    39361 ttgaattgac gaatgaagta ttggatgata tttttatcgt ggcaatgaaa aatccatcct
    39421 acgatatgga aaagatcaac gaaactactt atcaagtacg attagataaa ggacagtctt
    39481 taacgataac aacaaacgaa aaagatcaaa cagtcttgtc gatctcatct ggagactacc
    39541 cacgatctgt cattgatttc tttatcaatt acgaattctt ggtcccatta atggtgagaa
    39601 agtcctgatt ggataagttg gattaccaga tgtcattcag ataattctgt tccaaaggac
    39661 ttgcggcagt tatccagaat aattgtcaca agtcttttct gtatgtacta aatggacgtg
    39721 aataacttgc tgatgaagga gggatttcat taggaatgtc cacaaataaa atagcacagc
    39781 ttagcttatt gtctgctgct tgtgtagcag gtaggatcgc ttttcaattt attccgaatt
    39841 tccagccaat gactgctatt tttttattta tagtacttta cttaagctta aaagatgcgt
    39901 taattgtcat gagtctttct attgcgatca gcagttttta ttttggtgtt ggaccatggg
    39961 tcatcggcca atggatcagc tatactgttg ttctgagttt gttttcaatc gtctgcttga
    40021 gaaaaacagt gcaaaggaat ctatggttaa agggagtctg tttttttctg gcgggcatgg
    40081 tttacggaat aggtatgacc gtttttgata cgctgttata tcgtttgcct caaccttgga
    40141 tctattacct tcaaggggtg tcgtttgatt tgatgcacag tataggcaat gttgtgttct
    40201 ttcttgtttt tctgccagtc gtcaaacgtt tttacaatca ttcaggagga aaaaatgaaa
    40261 aaaataattt atagtttgct tgcaatgagt gtcagtatac tcattttgac aagttgcagt
    40321 acaacctata cacaatcggc ttccaaccaa aaggaggaag taaaaacatc gcaaaaagca
    40381 acaatcacgt tacaagaaaa tggtcaaaac ttttcggaaa aagaagtggt cttcaaaaaa
    40441 ggcgatgatt tactgagtat tttgaaacga aattttgaag tcaaagaaga taacggcttc
    40501 atcacttcgc ttgaagggca ctcacaggat gagaaaaatc aactctattg gatgtttact
    40561 gtggacggga aaagtgctac tactggtgcc aaagagatca aattgaatga tggacaatcg
    40621 gtcattttca atttgtctaa actataagtt tcataaaaaa ctgttaaagg gcgaatattg
    40681 ccataattca gtgagttcag ttgatgaaag aaggaaaata acatggaaaa ttttaaaaaa
    40741 agcggaatgt tttccgtaat tgctgctcta ggggctaata ttctagttgc tatcagcaaa
    40801 tttgtcggct atgcaatcag tggcagtgcc gccatgctaa acgaaagcat ccacagtatc
    40861 gttgattgca gcaatcagat tttgctgtta ttcggtgata aacaagccaa taaaggacag
    40921 agcgagttgc atcaatttgg tgaaggacga gcaaaatact ttttcagtac aatcgtcgca
    40981 atgatgcttt tcttcggcgg tggggcactt ggggtgatgg aagcttttga aaagttgacg
    41041 catccatccc atgaagtggg taatccgatc attgttatag caatactgtt atttggtttg
    41101 gtaatcgaag gaagttcgtt acgcgtggca atgaaagaaa tcaaagaatt gaatactgaa
    41161 aaactttcca cgatgcgctt tttgcgcgaa agtcgacaca gtgaaatact gattattttt
    41221 acggaagact tgtgtgctgt aatcggtttg gtcttggctt tagcaggaac aatattgact
    41281 tatgcaaccg ggattgctgc atttgatgcg atcagcggat tgctgatcgg tttcttgctg
    41341 atgggagccg ccattttcct agcaaaagaa ttttatagtc tgatcatcgg tgaaagcgtg
    41401 acagcaagcg atttatcaaa aatcaagtta gcttttgaac gtcctgagat cagtcggctg
    41461 atcgatataa aaacagttca tttaagtcca acggagattt tagttgcagc aaaagcagat
    41521 atcgttggta acaaagagaa tgaagcttac tcggtcatca atgatattga aaaaaatatg
    41581 cgtcagtctc ttcctgacaa gaagatgtat atatacattg aaacagataa atatgaccca
    41641 aattattctc gtaaataaaa cgtttattca tgaattgaag caaaaatgag aaaaatataa
    41701 tcttgttttt aaataaaacg gctacaattt agcgaattta tgttacaatg agtccgctgt
    41761 ttatttgttg taattaaagg agatatttta atgaacaaaa agaaaagtgc gccatggctt
    41821 caagctttgc atgcagtgat gccactttgt ataagctata taccagtagg attagcttgc
    41881 ggagtattgt tgcaaaaagt tggatttgat cctctcttat caggactgtt atcgattctg
    41941 gtcttttccg gtggcgcgca gtttttagtt gcatctatgc ttacgacgca agcatcattt
    42001 gcaacgacat tattgatggt tttcttttta gaattacgct atacattatt aggagcaagt
    42061 cttgccggtt ttatgaaaaa agaaaagcgg acgtttttgg ctgtcttttc tcaatcactg
    42121 aatgatgaaa actatgcggt gaactactta aagttttcaa ctgacccttc ctggaataaa
    42181 agaaaggctt tatatgtcaa ttggttctca atgactgcat gggccggtag caatatgatc
    42241 ggtaattttt tcggctcggt gattcgagta gatgctgacc ttgtccattt tgctttgacg
    42301 gccatgttca ttttcatgtt tatcatgcag atgaaaagcg cactactgat ctttacaggt
    42361 ttattctcag gcgtattagg ggtcgtattc atggtattat tccaaaatac tttcggattg
    42421 attgcagcaa cagtcattgc ttcatttgcc ggatttatgc tggagaaggc attcaaaaaa
    42481 gaaaaagcta agaaactaag agaccgcggt aaagatgtca atgagccgct cttcaacttc
    42541 ccggaacagg aggccaatca ccatgactaa actatatttg ctacttttag ttgcttgtct
    42601 gttcatcgtg gcatatatcc cgcgcttgtt cccgatgctt tattttactc atcgaaaagt
    42661 gccatcctgg ttcagtgact ggatgaaata tgtaccggtg gcattatttg cggcgttagc
    42721 ttttaaagat gttttcatta ctcatgaaca tctggatatt gcttggaata tcaaaatagc
    42781 tgcaatgatt ctagtagcag gcgtggctta caaaacacgt tcgatggcgt tgtcagtttt
    42841 gacaggactt gcgtctgtct ttttgctgtc tatgctttaa actgttttat tttattaaaa
    42901 acagagattg cgacataagt tgttcaaact gatctatccg aactgcattt gcaacagtca
    42961 attctggatg caagtttatt cgtagggata aaaaagatta gtgttggctt atgatgcaag
    43021 ctctttttta ttttaggaat tagagaggga tttgtccttc tatgattttt tgctataata
    43081 gggaagatgt ttattttatg aaagaatagt tgagggaatt taaatgaata aaaaattgat
    43141 gcaaggaaca ttttggctaa cctttgctaa tcttctatgt aaagttttag gggtagtcta
    43201 tttgattccc tggctaagta tgatggggaa caatcaagat ggtatgcttg ctacgacatt
    43261 gtataatgtt ggctatcttc cttatggatt gttcctgatg cttggtacag tcggatttcc
    43321 aaatgcgatt gcaaaaaaag tagctgtagc cacaaaagaa ggagataacg ctgcttgtcg
    43381 gctgattttt agaagtacga tcaatatcat gtttgtcatc ggtatcgcct ctgctgcact
    43441 gatgtattta tttgctcctt tactagccgg aatcagtccg attgctaacg ttaataatgg
    43501 gatactagct atcagaagtt tatgcccatc tttgattgct atcccaattc ttagtgcaat
    43561 gagaggatat ttccaaggaa aaaatgatct gcggccttat ggaacttctt tgattatcga
    43621 acaggctgtg cgagtgatcg ttatcttggc tggtacttat tacttgcgcg tattgactga
    43681 cggaaccata ttagaagctg tattgatcag tacagtcgct tctttcttcg gtgggttagc
    43741 tgcgattatc catatgtata tcgtcggacg cagaaaagac ttcttcgaac ttaaagattt
    43801 tttgatttcc agccgttatt tcgaaagaga aaaccgatct gcctctgtct ctatcattcg
    43861 ggaaacacta ccgtttattt ttgtcggctc agtcattact atcgttcaaa tgatcgacca
    43921 agtaacgatg aaaccactgc ttcacttttt ccgtccagag atcgcagatc agcaactgga
    43981 attcttattc agccgtgctt ctgtcaatcc aaataaattg acattgatcc tgatctccat
    44041 ggtaggaaca gtagctataa gcagcttgcc aatattaagc acgatgaaga aaaaagatcg
    44101 gatacaaatc gaaaaaacag taggagatag tttttcgatt gcgttattga ttcttcttcc
    44161 ttcattgact ggaatgtctt tattagctgg accactatac acattgtttt ttggctatga
    44221 tcctgaaagt gtcggatatt tccaaatggc tttgctagct tctttgtttt tctctttatt
    44281 cacgatccta tctacgatgc ttcaatcttt gaatcaccat cgttttgcga tccgattaac
    44341 tgtagaagca attatcctga aagtcatctt ccaagtaatc ggattaggat tagttggcgg
    44401 atacgggatg agcttatcaa atggcttagc attcggtatt gtctttgttc gcggctatca
    44461 ctttatgtgc aaagaatacc gaatagcacc tttagcaaag atcagcaatt ttttcttaaa
    44521 aactttccgc tccaccctga ttatgcttgc agtatgtttt gccgttttct ttttgctgaa
    44581 tcagcgattg tcaatggaat caaaaagcta tgcaatgatt tattgtgtgg ctgtaggaag
    44641 tattggcggc ttagtatttt tattttctca attcggaaaa aatgggtttc gaatgttgaa
    44701 aaatgttaaa catcatagca gaagcaaaga ataggttaac taatcgtttt ttgctgtcac
    44761 ttgaaaaacg gcaactcgta tgattacgct tgtcgttttt ctattttcca attttagttt
    44821 catatctaat cttacttatg atatagttat aaggtctgga aaaattcttt gaaacgagga
    44881 aattaaacac atgaaacttg cagtagttac agatagttcg gcatatctgc cggaacgtat
    44941 caagaaccat cccgacttat tcgttattcc aatccctttg attatggatg ggaaaattta
    45001 taacgaagga atcgatatcg aagcggatga atattatgga cttttaaaaa acagtaaaga
    45061 ttttcctact acttcacaac ctgtcgtagg ggaagttttg ggtttatatg aggaattgaa
    45121 agagaaaggg tatgacacga ttttaagtat ccatctttct tcagggattt ctggctttgt
    45181 caacacatta tttgcgatga aagatgacat cagcggagta acggtcattc cttatgactc
    45241 taagatcact agtatgccga tgggacacat ggtagaagct gccttagact taaatgatga
    45301 aggtcagtcc cttgatgaaa tacttgcaca tatcgatcga atccgagata atacctatgc
    45361 ttatctgatt gtagatgatt tgaacaattt agtacgagga ggacgtttga caaatggtgc
    45421 agcgctgatt ggcagcttat taaaaatcaa accgatcctg acattttctg aagggaaaat
    45481 tgttttattc gaaaaaatcc gttcaagtaa aaaagcattt gctcgagctg aagaaattat
    45541 tggtagaaga aataacgaaa tacaaccgcc agtcaaactt tacgtgatac atgcaaataa
    45601 tttagaagta gcgattgcgg agaaagagaa attgcagaaa aaatatccaa atgcagaaat
    45661 cgaaattggt catttcggac cagtcattgg tacacattta ggtgaaaaag ccatcggcat
    45721 tgccatttca gcgcaataat tccttttatt caaaaaacat accaaaaata t
//
LOCUS       DACKAC010000015        45444 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.015, whole genome shotgun sequence.
ACCESSION   DACKAC010000015 DACKAC010000000
VERSION     DACKAC010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.015"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            271..1140
                     /locus_tag="I0B46_000895"
     CDS             271..1140
                     /locus_tag="I0B46_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAR2083215.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1349..2374
                     /locus_tag="I0B46_000896"
     CDS             1349..2374
                     /locus_tag="I0B46_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAR2083216.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2413..3363
                     /gene="manA"
                     /locus_tag="I0B46_000897"
     CDS             2413..3363
                     /gene="manA"
                     /locus_tag="I0B46_000897"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAR2083217.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            3457..5160
                     /locus_tag="I0B46_000898"
     CDS             3457..5160
                     /locus_tag="I0B46_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAR2083218.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5162..7195
                     /locus_tag="I0B46_000899"
     CDS             5162..7195
                     /locus_tag="I0B46_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAR2083219.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7206..7646
                     /locus_tag="I0B46_000900"
     CDS             7206..7646
                     /locus_tag="I0B46_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAR2083220.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(7846..8973)
                     /locus_tag="I0B46_000901"
     CDS             complement(7846..8973)
                     /locus_tag="I0B46_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAR2083221.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8976..9653)
                     /locus_tag="I0B46_000902"
     CDS             complement(8976..9653)
                     /locus_tag="I0B46_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAR2083222.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9917..11683
                     /locus_tag="I0B46_000903"
     CDS             9917..11683
                     /locus_tag="I0B46_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAR2083223.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11698..12135
                     /locus_tag="I0B46_000904"
     CDS             11698..12135
                     /locus_tag="I0B46_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAR2083224.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12148..13299
                     /locus_tag="I0B46_000905"
     CDS             12148..13299
                     /locus_tag="I0B46_000905"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAR2083225.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(13361..14059)
                     /locus_tag="I0B46_000906"
     CDS             complement(13361..14059)
                     /locus_tag="I0B46_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAR2083226.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(14052..15404)
                     /locus_tag="I0B46_000907"
     CDS             complement(14052..15404)
                     /locus_tag="I0B46_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAR2083227.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(15627..17027)
                     /locus_tag="I0B46_000908"
     CDS             complement(15627..17027)
                     /locus_tag="I0B46_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAR2083228.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            17188..17925
                     /locus_tag="I0B46_000909"
     CDS             17188..17925
                     /locus_tag="I0B46_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAR2083229.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            18050..18529
                     /locus_tag="I0B46_000910"
     CDS             18050..18529
                     /locus_tag="I0B46_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAR2083230.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            18526..19293
                     /locus_tag="I0B46_000911"
     CDS             18526..19293
                     /locus_tag="I0B46_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083231.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            19523..20092
                     /gene="lepB"
                     /locus_tag="I0B46_000912"
     CDS             19523..20092
                     /gene="lepB"
                     /locus_tag="I0B46_000912"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAR2083232.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(20196..20798)
                     /locus_tag="I0B46_000913"
     CDS             complement(20196..20798)
                     /locus_tag="I0B46_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAR2083233.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            20904..21373
                     /locus_tag="I0B46_000914"
                     /pseudo
     CDS             20904..21373
                     /locus_tag="I0B46_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            21479..22063
                     /locus_tag="I0B46_000915"
     CDS             21479..22063
                     /locus_tag="I0B46_000915"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAR2083234.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            22066..23139
                     /gene="prfA"
                     /locus_tag="I0B46_000916"
     CDS             22066..23139
                     /gene="prfA"
                     /locus_tag="I0B46_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAR2083235.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            23132..23971
                     /gene="prmC"
                     /locus_tag="I0B46_000917"
     CDS             23132..23971
                     /gene="prmC"
                     /locus_tag="I0B46_000917"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAR2083236.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            23964..24143
                     /locus_tag="I0B46_000918"
     CDS             23964..24143
                     /locus_tag="I0B46_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083237.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            24165..25190
                     /locus_tag="I0B46_000919"
     CDS             24165..25190
                     /locus_tag="I0B46_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAR2083238.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            25365..26609
                     /locus_tag="I0B46_000920"
     CDS             25365..26609
                     /locus_tag="I0B46_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAR2083239.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            26762..27709
                     /locus_tag="I0B46_000921"
     CDS             26762..27709
                     /locus_tag="I0B46_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAR2083240.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            27843..28472
                     /gene="upp"
                     /locus_tag="I0B46_000922"
     CDS             27843..28472
                     /gene="upp"
                     /locus_tag="I0B46_000922"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAR2083241.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(28611..28853)
                     /locus_tag="I0B46_000923"
     CDS             complement(28611..28853)
                     /locus_tag="I0B46_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083242.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            28974..29046
                     /locus_tag="I0B46_000924"
     tRNA            28974..29046
                     /locus_tag="I0B46_000924"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29007..29009,aa:Thr,seq:cgt)
     gene            complement(29165..29803)
                     /locus_tag="I0B46_000925"
     CDS             complement(29165..29803)
                     /locus_tag="I0B46_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAR2083243.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(29926..30558)
                     /locus_tag="I0B46_000926"
     CDS             complement(29926..30558)
                     /locus_tag="I0B46_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAR2083244.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            30909..31208
                     /locus_tag="I0B46_000927"
     CDS             30909..31208
                     /locus_tag="I0B46_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAR2083245.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            31703..33868
                     /gene="acm"
                     /locus_tag="I0B46_000928"
     CDS             31703..33868
                     /gene="acm"
                     /locus_tag="I0B46_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAR2083246.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(34140..34625)
                     /locus_tag="I0B46_000929"
     CDS             complement(34140..34625)
                     /locus_tag="I0B46_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAR2083247.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            35105..35746
                     /locus_tag="I0B46_000930"
     CDS             35105..35746
                     /locus_tag="I0B46_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAR2083248.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            35765..36400
                     /locus_tag="I0B46_000931"
     CDS             35765..36400
                     /locus_tag="I0B46_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAR2083249.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            36418..37233
                     /locus_tag="I0B46_000932"
     CDS             36418..37233
                     /locus_tag="I0B46_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAR2083250.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            37552..38988
                     /locus_tag="I0B46_000933"
     CDS             37552..38988
                     /locus_tag="I0B46_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAR2083251.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            39177..41624
                     /locus_tag="I0B46_000934"
     CDS             39177..41624
                     /locus_tag="I0B46_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAR2083252.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            41854..42921
                     /gene="rlmN"
                     /locus_tag="I0B46_000935"
     CDS             41854..42921
                     /gene="rlmN"
                     /locus_tag="I0B46_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAR2083253.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(43114..44217)
                     /locus_tag="I0B46_000936"
     CDS             complement(43114..44217)
                     /locus_tag="I0B46_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAR2083254.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            44770..45318
                     /gene="aac(6')"
                     /locus_tag="I0B46_000937"
     CDS             44770..45318
                     /gene="aac(6')"
                     /locus_tag="I0B46_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAR2083255.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT        15541 a         7791 c         9279 g        12833 t
ORIGIN      
        1 ccttaaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatag
       61 gaggagctat ttttgtttgg ctgttcttat tacttgatgc agaacagctt tttcacaacc
      121 tctttttata agagttttta ctcattaatt tacaaaaaac aagcaaaatg cgaattttct
      181 cacatttttt ttaacaaaat caaagttatt gcttgaaaga agaagaatcc atcgttaaaa
      241 taaagcttga cgtagagggg agcaagaaat atgaaagatt ttcaattggg ggctgccacc
      301 tatttaaaaa gtgtagccat tcgggtgaaa gaccgagata aaatgattga tttttacaag
      361 aacgttgttg gctttgattt gaaaagagaa gaaaacgaat tagccatttt aggaacagaa
      421 gaagctggaa gtgaaatgtt gctgctggaa gaaagtccta gagcgaatga tcacatgggg
      481 gtgatcaaaa agctgaaccg tttttcattg attatcccta gcacagaaga aatggcagat
      541 atcctttgtc gtatccgtaa aatgaattat ccaatcaaag aagctttaga agatcatgga
      601 cgtatgggga ttttgctcgt agacccagaa gagaatgaat tggaaattta ccatgaagag
      661 aataagaacg accaaagaga aattccagaa acaatggatc aagaagcatt attaagaaaa
      721 tctgaagaaa aattcagtaa actatcaaca ggttcttatt ttgaaaaaat ccatttaaat
      781 gttactaact taacaaaaga acgccaattt cttaatcatg tacttggttt gaaggtacat
      841 aatgaagaag agggcatcca tgtattgaat gaaggagatt tccatgttgg tttgacagaa
      901 gctagcggag gcgcaattga tttgcctaca gatgaagtga ttggacttga ttttctgaaa
      961 ttcatgatcg atactgaaag catggaagga ctgattgctc acttgcagca actatcagtt
     1021 gatttcttct tagacaaaaa gcacacagta ctcacaatct atgatgctat aggaatcgaa
     1081 tggtggttcg tcagtgagcc aaaagaaaag aataaaactg acattcgatg cgacgcttaa
     1141 ctttttcttt cttaacgatt gtcgcatgtc tcgtcatttt atgaacggtc atactatcgc
     1201 ttattctaga gatagaaaga gaaaatagta taccggggga aactgcgcgg tttttcgcgc
     1261 agtttttttg tgtaaaaaag gttgattcct catagattaa gctttaagct tggactgtaa
     1321 gaatctgata gaaaagcagg agaaaaagat gtatccacac acgaaacagc ttattttgaa
     1381 aaagatggaa gaaggatttt atccaggtgt agcagtcgcc ttcataaaag aaaatcaagt
     1441 agaaacattt acagccggct ttgcccaagt cgttcctgaa aagcagctga tgacagaaga
     1501 tctgttgttt gatgcagcct cactgacgaa agtcgttgca accacttcat tagttttacg
     1561 tctattagaa gatggaaaga tagaactcga tcaaccgctt cataaatatc ttcctgaatt
     1621 tgaaaatcag acaatcactt tgcgtcattt actgacacat acttctgata tcaaaacatg
     1681 gataccaaac agagataagc tatctcagga agaattgatc gccgcttatt tgaagctaga
     1741 accaggtgag caactaggaa aagaagtaaa atatacagat gccggtacga ttcttcttgg
     1801 gttgatgcta gaggaaattt accaagaaac tgcagtggaa atctttcaag aacaagtatt
     1861 ggaaccatta ggaatgatga acagcttatt tcttcctttt ccttccaaaa aaatcgttcc
     1921 cacagaacaa ctgccttcgg gtgaggtttt gcgtggacaa actcatgatc cgaaagcaag
     1981 agttttggct gaacatgcag gtaacgcggg tctctttatt gatttgaaag acagcattac
     2041 ttttgtccaa atgtatttga acaagggaaa aatccgtaat ggtcgttttt tagaagaaga
     2101 gacgattgat tctttactgc aagatcaaac accgaaaaaa aatggctatc gttctttagg
     2161 atgggactta aaaaaggatg tagaagacgg tcatgcgatt ctttttcata ctggttatac
     2221 aggtacgttt ttcttgattg atataaaaac acaagaagcg ttgatatttt tgtcaaatcg
     2281 cgtacatcct gtcgatcatc gtgcggagta tctcatatgt cgagatgaaa ttgttgaaaa
     2341 ctttttgaaa gagaaagcgg tattaccgaa ataatgctat aatagctatg aaaaatgaga
     2401 ggagtttgaa ttatgcagga acctatgttt ttaaaaccag tttttcaaga aaaaatttgg
     2461 ggaggaagtc gtttacgttc agtctttggt tttgatatcc ctaacgataa aatcggagaa
     2521 gattgggcga tcagtgcgca tcctcatggt gtcagtgtag tagaaaatgg cgaattcaaa
     2581 gggaaaagat tagatgaact atggtcagaa cacaaggaat tgtttggcca tccttcagaa
     2641 ccagtgtttc cattattgat caagatctta gacgcagaag atgatctatc tgtacaagta
     2701 catccagatg atgcctatgg gatgaaacat gaaggtgaat taggaaaaac tgaatgctgg
     2761 tatatcatcg atgctgaacc tggtgcagaa atcatctatg gtcatcatgc aaagacaaga
     2821 gaagaactag ctgagatgat caaagatggg cgctgggatg accttttgaa gaaagtacca
     2881 gttaaaaaag gtgatttctt ttatgtacca agcggaacga tccatgctat tggtaaagga
     2941 atcatgattt tagaaacaca gcaaagttct gacacgactt accgcgtgta tgactacgac
     3001 agaaaagatg cgcaaggaca aacaagagag ctgcatatcc aacaatcgat cgatgtaaca
     3061 actgtccctg cgaaaacacc agaattacaa atcaaagaag tccgaaaagg gaattcatcg
     3121 atcgtcactt atttagaaac tgagtttttc aatgtatatg agtgggatat taaaggaatc
     3181 acatctttca aaaaacaagc cccttataca ttgatgacgg taatcgaagg agcaggagac
     3241 cttgtcgttg acggaaaaac atatccatta gaaaaaggaa caagctgtat tattccaaat
     3301 ggcgtttctg agtgggcggt ccaaggtgaa ctagcaatca ttgcgtctga accaggcaaa
     3361 tagttcccag atagctgaaa gcagcagacg gtttttcgtt tgctgctttt tctacataaa
     3421 atccgtccat caataaatag aagtctggtg aaaaagatgg aatacaaatg gaaagcaaac
     3481 aacagcagtc gcgtgtctat gaaacaactg agaaaagctg gacatcactt aaatgaaatg
     3541 atcatgccaa atctaagtct gtttatcgct tggggaatct tgtcattagc aacaaaaggt
     3601 gtgtctggcg atttgcacac cacattagaa gaggtatgcc attggatgat ccagctgctt
     3661 ttacccatct tgatcagtta ttcaggcggg aaatcggtag ggggacaaaa aggggcagtc
     3721 gccggtgcaa ttgcttcttt agggttgatc gctcgttcca atgctccaca aatcgtagga
     3781 gcgatgatta tcgggccgct tgccggcatc gtatatgaac aatttgtttc tagattccat
     3841 gtgaaattca aggctggata tgaaatgctt gtcagcaacc tactagtagg actgactggt
     3901 agcgttattt gtataattgg aatcgttttt attgaaccag ttttaaatag tgttcacttg
     3961 ttgctcgcct ccgttgtgtc ttggctagtc acaatgaatc tgttaccact tgtcagtctg
     4021 atcttagaac ctctaaaagt actatttttc aataatgcaa tcaatcatgg tgtattgaca
     4081 cctttaggaa tcgaattctc acaaacagtc gggtactctt ccttattttt gatggaggca
     4141 aatcctggac ctggtttagg gatcttactt tctatccttt gttttgcaga aaaacaggaa
     4201 aaagtaaatg cgagtggagc attgatgatc catgcgattg gcgggattca cgaaatctat
     4261 tttccatttg tcttgctgcg accgtatcta tttttagctg taatggctgg tggggcaagc
     4321 ggcacgttga tatttcaatg gttagatacg ggattagctg cacctgtatc gcctgggtca
     4381 ctactcgtga tttttgccaa tgcgccttat ccacaactag ctggggtaag tatagggata
     4441 gcaatcagta catttgtgac cttcatttgt gcagcgatac tattgaaatt accaaataaa
     4501 gaaaaagaga acaaaacggt caaagaaagg ggagaaatct tggaaccatc tatcattcat
     4561 tccgtcattt ttgcttgcga ctcaggtatg ggatcaagtg ctatgggagc cgccctgctg
     4621 aaaaagaaat tagcagcaga aaaaatcgat ctttctgtcg gatacagctc tatctatgaa
     4681 ttgaataacc aagtaaatca gctggttatc gtgcaacatg aactagcggg tcttgcaaga
     4741 gaaaaagtac ctcaaagtca aatctatgta ctggatcact ttttggaaac agatatcttg
     4801 ctgtcacgaa taaaagaagc gatccaattt ccaaagcggg aagaaaaaaa agagatagca
     4861 gatatcacag aacaaaaaac gtcgcctgct aatattgtat ttttgtataa aaacaacaga
     4921 cgcggctcac aaactatggg catgacgatt ttaagtcaat tagcaaaaaa agcgaaaaaa
     4981 cagtttgtta tctcaaaaga atcaatagaa aaaatcaaaa tccaagaaga aacgatttat
     5041 atcgcacaaa gagaactagc tgtaactgaa cagctagaag aaaagatccc tcggctagtt
     5101 ctagttgaac attgggtagc tacgaatgaa tacgaaaaac tcgtgaaggg agaaatataa
     5161 gatgtattta tcgcccagag aaaaacaatt attagcagaa tttctcagta gcccgtcacc
     5221 tgtatctatc caaaaaatga tgaatttatt gaaggtcagt aaacggacta tttaccgcga
     5281 attagataat cttgaagtct cattgaaatc ggtaaatgct tctttgaaaa aagcagctag
     5341 aggctcattt gtcattgagg catcagaaga agtattagag caattaaaag aaaatacaga
     5401 tgggaaaagt aatgacttat ccactgctaa acgacaacac gcgattttag ccgagttgtt
     5461 actgacgaca gagccgttga gtcttgctca tttcatggaa tcatatcgca tcagtaatac
     5521 cactttttat gcagatatca aacagttgga agaaagtatt cgccaactgc ctttggaaat
     5581 cattcggaac cagggatacg agatcgaagg accggaaaaa taccgccggc ttctgacagc
     5641 gaatgtatta gaattagaaa tcaatgaata tgaacttttt catagtattt ctttcgatgc
     5701 atcactgaat tatttttttc gattcgttga tccgcaacat ctatctcttg cacgcaaagt
     5761 aatcggggaa gaattgatcc aaaaaaagac caatctaagt gatagaaagc ttgaacattt
     5821 ggttttgatg ctgacattga cgatggagcg tgtgacgaaa aatcatttac tgacaaatga
     5881 gacatataat agtctggcaa acaaagaatt actacagaca gctaaacgat tgttttcacg
     5941 gatcgcttct gagacaaaac aactttatcc agtaaatgag atcgtctttt ttgcaggatt
     6001 attaaatgac ttcacgaact catttgatca gaatttcttt gaagagacgt tcgatacaca
     6061 gcttgcttat ttagtaaaac aactgattca agaagtaagt gaagagacga atgtccattt
     6121 ttatgaagat gaaactttgt acaaaatgct gttgactcat ttatctggtg tgttttcaag
     6181 agccgtttta caagaagaga cactgaccaa tccgatttta gaaaaaatca tggttcaata
     6241 cgaggaagtt gcgaatgcaa tccgtaaagc agtagagcgg atttttcaag aaaaaaaatt
     6301 atccgaagaa gagatcgctt atatggtcct tcattttgcg aattcattgg aacgaagtcc
     6361 gaaagaaagc aatatcagta ttgcaggatt ttcgccaagt ggattagctt ctactagcat
     6421 gttggaacta cgattgaaaa ggtattttcc cttcatccaa caaattcatt tctatcggat
     6481 tgctgattta aaaaaaatcg atttacaaga agaatatgat gttgttattt ctacatctat
     6541 tttaccagga tacggtggaa aatatatttt agtctccccg ctactgctag aagatgaagt
     6601 caagcagcta aaagaagctt tttcgcatat tgagaagaaa aaaaggcaaa aaattacgta
     6661 taacagcaat aacgagtgga tgatagaaga tacttatgag gaaacaatgc atttcatgga
     6721 tcaaatcaat caattactcg gtcgtttttt tatccaagaa ctggataatc ctgagacgat
     6781 ttccgaaacg attcgcagct tatttcctta ttttcctgaa ggactagtaa cggatgaaga
     6841 ggtggttcat cgccggctgc ttaagcgata tgaacaagca cctatcggta ttcctcatac
     6901 aaatatgggg ttattccata catccagctc attgatttct aaaccgatct tttgtatttt
     6961 caatttgaaa catccgttga ccattgaagg gatggataga cagccagtcg atttgtcccg
     7021 tatgttagtt atgctggcgc cttcgccaat ccaagaaaaa gacgggattc ttttaggaaa
     7081 gatcagtgga gcaatcatca tgaatgactt gaacaccgag atcttccgct caggaaataa
     7141 agaaatcgtt tatcagttgt tagcgaaaat cctgattgaa gaaataaaga aatgaaggtg
     7201 aaaagatgat acagctacca gcagaaaaaa tattgctcga tcaatcctat caaaattgga
     7261 aagaagcgtt gaccgagatc ggccagtgga tgacccaaca agggggaatc aaaccggcat
     7321 atatccaatc aatgatcgaa agacaagaaa aagcgagtgt ctatatcggg aatttcgtag
     7381 ccttgccaca tgggactgac aaagatgaag aagtagtaga agaaggaatc tatttagtcc
     7441 aagtgcctga tggtgtggac tttggaacaa aagaggaacc aaaaatcgcg acactcttat
     7501 ttgtagtggc tttaaaaaaa gaacatcagt tagcggtgct ccaagattta gcctttttct
     7561 gttcagacat cgaccaagta atggcgctca gtgatgcgga aaccattgaa gaagtccaaa
     7621 tgatttttga acatggacat atataaccaa tgttcacagt aaaaaaagct gccttgcatc
     7681 aagtaataac ttcaaccaag ataaaaataa gaggagctat ttttgttggg aggttcttat
     7741 tactgcggat gcaagctttt gaaaactatt tattctagaa aaccgtttat ttgaaatagg
     7801 ggacggtgac aaggtttttg tcaccgcccc tattattttt gtgttttaat agctcttttt
     7861 tatatagata gtgcccatac ctatgaataa taaaacgata ctccatatac caagtgtcaa
     7921 gataccattc atgaaagtaa cctgttctga agctagtggc gagaaaatat ctcctcctat
     7981 gatcacattt tgaaaatcaa cataagaagt tggcaagtaa tgactgattg attctatcca
     8041 tccaatcgaa gaaaacaagc gatactctgc taagaaaaga caaagaagtg tacctaaaag
     8101 attgactaga gaatgttcaa ataaagcaga tagaagaaaa ctgattgtac ttaaaaagag
     8161 cacccacaac aacaggaaca gaaagctctg gaaaagatac tcatacatcg gcaggattcg
     8221 tatctcctga ccaatgatcg ttcctacagg ataacggaag tctcccaaac catttttgat
     8281 tattactagt atagagacga tcaaaaatgg catcacgaga cttagcatag ctgcaccata
     8341 ataaatggta aaacggatgc tatgtttctt taccatacta tccggtaata gattgactag
     8401 agagattacc tgctttcttt tttcataaga gatgatggaa gaaagaaaca aggcggttat
     8461 acataaaatc aaaaaactag aaatcattcc tgaaaaaata ttttcgtagt aatttgctaa
     8521 agagagatgc gctggcaagt caaaaataga atatctttgg atctgatgtt ctttcatata
     8581 acggagcaac tcgacacgtt tttgttgttc aatgattgga attccttgtc tttgaccaga
     8641 aatcagtcga tccagtatat ctttttcata atgataagtt gcttcgacca tttgctggac
     8701 attccctgtg ttttttgcat gtaagattgc ttcgatcagt ttgttgcttt tctctaaact
     8761 ttccacaaat tctttctcat tctcatcttc tttcaacgtt tcaatcaaac gttgcatctc
     8821 ttcatagtta tgattcagtt catcgaattc tgtataagca agtttatgat caaaaaaaag
     8881 tgggaatgca aagagcacga ccaacaatag aggaaataaa aagtttactt tgttttttag
     8941 taagagattg cgttcaaatg taagtaaaga catagttaca cctcatataa tttacgataa
     9001 atagctaaaa tctcttccat atttgaggat tctgtgacga tttctccatt ttttagaaaa
     9061 catgctttag aacaaatcga ttggatattc gcgatagcgt gggaagaaag aagaatcgtc
     9121 ttcccctctt tctgccattg tctgatgagg ctattcaaga tatcaatact tcctgggtct
     9181 aatccgttca acggttcatc gaataacatg acagatgaat cactgatcaa gtaagcagca
     9241 agcaagacat gttgtttcat tcctagagac atttttccaa tcggcaaagt aaggtactca
     9301 gtcatcttca atcgttggaa aacagtttgg cattctactt tgctctgcca atagtatttg
     9361 atcaaagtca aataatcaac agccgataga tagtcggata actgattcgt tgattctaga
     9421 aaaaagaaag accggcgata atctgtataa gcagtaggag agatgttgtg aagggacaat
     9481 tgatcataag ttgttggcag cagttgggca atgccattca gcaaagttgt tttgccaaat
     9541 ccgttcgggg caatcaaagc agctacttct cctgattcta ctaaaagatt caattgtcga
     9601 atcacacgtt ttttaccacg tttcatgttc atgtttttga tcagtaatgt catagatagg
     9661 cctccaatat aaaatttaat aacataatag aatggaaaac atctaaatta aatgctaaca
     9721 aaagttattg tttgcatatt ataaaaaaat taaaataaca gttttattta aaattgaagt
     9781 tttctgatac cgaaatcttt ttattttgct gacatactca aacctttggc acaaagtttc
     9841 gtgccaaagt acgactattt ctctttttag taagcggata caattagagt atcaaaagga
     9901 gagagggaga gacaaaatgg aacggacgat tcccaaaaac aacatttcgc taaaagcgag
     9961 ggtacaaaaa ttaggcagta ctttatcaag catggtcatg ccaaatattg gagcactgat
    10021 tgcctggggt gtattaacgg ctctatttat cccagatggt tatttaccaa atgaggcttt
    10081 cgctaccatg gtaagcccaa tgctaactta tctgattcct ttattgatcg gttatactgg
    10141 cggaaaagta attgctggtg accgcggatc agttgttggg gcaattgcaa cgatgggtgt
    10201 gattgtcgga acagatattc caatgatgct aggcgcgatg atcatgggac ctcttggtgg
    10261 atatgccatc aagaaatttg atcagctatt ccaaaaacgg atcaaatccg gttttgaaat
    10321 gctagtcaat aatttttcag caggtcttat cggttttgga ttagcgttat taggtttttc
    10381 tgcgattggt ccagttgtcg atgcattgac tcaagcaatg gcaaaaggtg tagagatcat
    10441 tttatctgcc catttgattc cattgacaag tatttttatt gaaccagcaa aaatcttatt
    10501 tttgaataat gcaatcaatc atgggatttt gacaccattg ggaactgaac aagtagcaga
    10561 ggctggaaaa tctattctat tcttgcttga agcaaatcct ggtccaggac ttggcgtatt
    10621 acttgctttt actttgtttg gcaaaggagc agcaaaatct tcagcaccag gagccatgat
    10681 cattcacttt ttaggcggga tccatgaaat ctattttcct tacgttatga tgaaaccgtt
    10741 attattctta tcagttatag gcggaggaat gtcaggaagt ttcattttcc aattgatgga
    10801 tgctggacta agagcgccag cttctccagg gtcgatcatt gcgattctag cgatgacacc
    10861 gttcaattct taccttcctg ttattttggg cgtgattgtt gccgcagctg tttcgtttgg
    10921 tatttctgca gtaattttga aagcggatgc gaaagataca aatgaagact tcgaaaaatc
    10981 tgttcaagag acaaaggcag ctaagcaagc ttccaaaggt gcccaaggta ttcctcaagg
    11041 agctacattc tctggcgtac aaaaaatcat ttttgcttgt gatgcaggaa tgggatcaag
    11101 tgcgatgggc gcttcgatct tgagaaaaaa agtacaggaa gcagacttgc ctcaaacggt
    11161 gaccaatcaa gcaatcagcc aactgacaga tgactcgaat acattgatcg ttactcaggt
    11221 cgaactacaa gaaagagcaa aacaaaaagc accgagcgcg cgttttgtct cagtagaaaa
    11281 ctttttgaat tcacctcgat atgatgaaat cattcaagaa ttaagcagca agcagaaaac
    11341 atcagtttct gaaattgaga atgagccagc agaagagttt ctttcagcaa acatcaaaca
    11401 aatcaaagag attctacttg tccacgatga tcgttccgga tcagcaacaa tggggatgac
    11461 cgtgctcaaa gatatattag acaagaacca tttaactatc ccgttaagaa aagtcaatat
    11521 ccaagattta aaagatgaac ctgattcact gatcgtagcc aaagaaagtt tagcggaaaa
    11581 agtacagatg gctgcacctc aatccattca tttatcagtc aacagcatgg tcaatccgca
    11641 aaaatacgaa tcgattgcac aatatttgaa acagacagct taagggagga aaacagcatg
    11701 caaagtttgc cattcgacat gatcgagctc aatcgatctt ttaaaaccaa agaagaagcg
    11761 atccgctatt gcggtcagat gcttgtagaa gcagggtgcg ttgatgagga atatatcgat
    11821 gccatggtag atagagacaa aatgttatct gtttatatgg ggaattttat cgcgattcct
    11881 catggaacag atgaagcaaa aaaatatgtg aaaaaatccg gcatctgtgt cgttcaagta
    11941 ccagatggtg tagattttgg taccgaagaa gaagaaaaga ttgcgactgt tttgttcggt
    12001 attgcaggag tcggagatga gcatttgcaa cttgtacagc aaatcgcact ttattgcagt
    12061 gatatggaca atgttgtcca gctagcagac gcgctcagca aagaagaagt aacggaaaat
    12121 ctagcaattg cttaaggaga gagaaaaatg agagcagtac attttggtgc aggaaatatc
    12181 ggtcgaggat ttattggaga ggttttggca gctaatggat ttgagattac tttcgtagat
    12241 gtgaatcaca cgatcattga tgctttaaat gaaagaaaag aatacgaaat cgaattagca
    12301 gatgaatcaa aagaaaaaat cactgtaaaa aatgtcaaag gaatcaacaa tcaaacaaat
    12361 ccagaaaaag taatccaaga aatcgctgaa gcagatttag ttactaccgc cattggcccg
    12421 aatatcttac cttttattgc tgagttgatt gcaaaaggta tccaagaaag agaaaaggaa
    12481 ggaagcacaa catctttaga tatcattgcc tgtgaaaata tgattggcgg ttcagcattt
    12541 ctagaaaaag aagtgtataa atatcttccg gaaacatcat ttactgacaa atatatcggc
    12601 tttccaaatg cagcagtcga tcgaatcgtc ccattacaac accatgaaga tcctttattc
    12661 gtccaagtgg aacctttcaa agaatgggtc attgacgata gccaaagaaa aaataaaaaa
    12721 atccaattaa aaggggtttt atatgtagat gatctagaac catatatcga acgcaaacta
    12781 tttagtgtca atactggtca tgcgactgtc gcttatacag gtgccttgtt aggttaccag
    12841 accattgatg aagcaatgca agatgcgtta gttgtggcac aattaaaatc agttttgcag
    12901 gaaacaggaa gtttgctgat tgccaaatgg gggtttgatg cagagcagca tcatgcgtat
    12961 attgaaaaaa tcattcatcg cttccaaaac aaaaatatct ctgatgcgat cacacgtgtc
    13021 gctcggacac cattacgtaa gttaggttat caagaaagat ttacacgtcc agtgagagaa
    13081 ctacaagaac ataacttgac ttgcccacat ttgacggcaa caatggggat tatttttaat
    13141 tactatgatc ctgaagatga acaaagccgt caattgcatg aaatgaagat ccatgaaaac
    13201 ttggaacaat tgattcaaga agtcacagga atcaatgatc cgaaaacaat cgggaatatc
    13261 aagcaaaacg tgaaccgtta tgccaagcaa gtcgcttaaa ataggatacg aaaaagggga
    13321 atgtgacgaa atttttgtca cattcccctt tttcgaataa ctaggctgtt tgtccttcaa
    13381 gatagggcag atccaattct ttaccgtaac gttgttctaa agctaggcgg atcaaacgag
    13441 ctgccaggtt ctcatttctt gcaaggatag gtccatggaa ataagaaccg aagacatttc
    13501 ggtaaatgac accttcagat tggtcttcac cattatttcc ttgaccttgt accactttcc
    13561 ccaaaggtcg ttcaccttca cctaagaaag tccgaccatt gtggttttca aatccataat
    13621 aagtttcatt gaattcttcg ttatggataa cgatatcacc gataaaccgg ttattttcct
    13681 gacttaacgt gtagtgatcc agtgcactga ttccttggat cttttctcca ttagctccca
    13741 tgtagtagtg acctagcaat tggtagccac cacaaatggc taacatgacg ccatcatttt
    13801 cgatatagtt gatcaaagaa tctttctttt tctggatgtc ttttgaaatg attaattgct
    13861 caaagtcttg accaccgcca aagaaaacca agtcatattt atcagcatca aaaggttcat
    13921 gtatactaac gatctccgta tggcaagaaa cccccatttt ttctgctata tatttcaaca
    13981 tcaaaaggtt tccgttatca ccatatgtgt tcatcaaatt tccataaagg tgggcgatat
    14041 tgagttcata attagccacg gttcattcct cctttgataa atccttgagc agctagttct
    14101 ttacgaagct gcaatacagc agtgtaagta gctaaaatat agacatgctc agttggcaat
    14161 tctttgattt tttcgattac ttctggtaat tcttttgttt ctgtcaattt attttctgga
    14221 atcccggcaa cttttagacg taaagtcata tctgtatgac ggtctcctcc agaaataaca
    14281 gctgggatat ccatatcggc aaagttttca aaattaccat cccaaatcca gctgacatcg
    14341 attccgtctg cgtaattggc atttaataaa gccactaaag aaaatggata aggagctaga
    14401 ccaatcatat cgattacttg attgatacct acaggatttt tgacaagtac taatgtacat
    14461 tttttcccat tgatattgat gacttcttgt cggccgaata ctttctcgtc atatcctaaa
    14521 ccttgtttga ttttatcagg atcgacattg aagtactctg ccactgcagt agctgctaaa
    14581 gcattgtaga cattatacat accgccaact tcgattccgt attcatgacc atcgatcacg
    14641 aaatctgctg acacattgtc catccgtacc atatcagtta atcgataatc caattcagga
    14701 cgtttgaagt cacaatttgg acagtaatat ttccctaagt ttgcataagt gatcatttta
    14761 taatgcaaga tgtgatgaca atttggacat aagacaccgt ctgtattgta atgagccatt
    14821 tgttcacgat cttcttcatg gtcaaagcca taatattttc taggattgac tgtttcgata
    14881 gaattgaaga ttggtgaatc accgttactg ataatcgttg catttggtga ctttgcggca
    14941 ccgtctacaa tcattttata ggtcgtatag atttctccat accgatccat ttgatcacgg
    15001 aaaatatttg taaataagaa cagttctggt ttcatatatt ctgtgacttt gctcaaactt
    15061 gcttcatcta tttctaggac agcaaacttt ttctgtcctt tttttggttt tgcctgcaag
    15121 aaagtcgata caattccctg taccatattc gcaccagtag ggttggttag aacttcatcg
    15181 aattgctggc gcaaaatatt gactgttaaa gcggtagtca atgttttacc gtttgttccg
    15241 gtgacaacga cgacttggta gtcttttgcc aaactatcta gtatatgtgg atcgactttc
    15301 agcgctagtt tacctggtaa acttgatccg cctttaaaaa aagtttttaa tgcccattgc
    15361 gacgtttttc caactgcaat cgcaaaatga ctgcgtattc ccattatgta acctccaaaa
    15421 acgatttgct gacgaataaa ttcaactaat cataccatat gttgaaaaaa aggttaaatt
    15481 gcttttctta ttcttaagaa aaggttacgg ctgaaagtgc atgaaatcgg acaaaatcgc
    15541 atgatttaac aaaaaaacag cttgaaacaa aatgattgtt tcaagctgct gtgattttat
    15601 tattccctct tacctatatt cccattttaa ccgataataa ttttctcgct tggatattca
    15661 tagcctagat ctcgtgtttc ttttggcacg aataacatca atgtatatag cattccgatc
    15721 cgcccaataa acatcagtgt agcaatcgtg attttgccca caacagacaa gtctccggta
    15781 atccctaaag ataaaccagt tgttccaaac gcagaagtta cttcgatgat gatcgagatc
    15841 agcgtttgtt cttctgtagc agataaaaaa acgatacaaa agaaacacat accaagagaa
    15901 agcataaaga caacgactga ttttcgcaca tcatcttgat cgattctccg tccaaaaata
    15961 ttgatgttat cttcactttt gaggaaggaa tatagataca aaccgatgat tgctacagta
    16021 gttgttcgaa tcccgccgcc tacagaacta ggactgcatc cgataaacat cagtaaagaa
    16081 aaaatgatca gtgttgtgat ttgaaaatca cccaaatcgt gaatctgcag accagcatta
    16141 cgagtcgtaa tcgaataaaa catcgaattg atccatttga cactcatatt ggaatcttgg
    16201 aataaatggt ccttttccaa tagataaatc aagacagttc ccgaaacaaa caagataaca
    16261 aaagctaaca cagctaattt ggtaaacaag gaaaaacgaa acggaagctt cgcattggaa
    16321 cgtttgtata atagccattc ccggcactcc atcaatacag gaaaaccaat tcctccgaca
    16381 aaaatcaaaa acatgatcag gatcaagaaa aaatagtcat gtgcaaaggg cttgatggaa
    16441 tccccagtga cgtcaaaccc agagttcgtg acagccgata tcgcctgata aaaaccgtaa
    16501 aaaacggcat cacgccaacg atcaaaatag cctctgtaat aaaaataaat agaaaagaat
    16561 gtcccaaaaa gtaattgaaa ccagattaaa atagcaaaag tgatccgaat cagtcggacg
    16621 ataccagaca aacgcggttg attcatatcc gtcatgatca gctgtcgctg tttcagtgtg
    16681 atccgtcgtt tagaaaaaat gacaaaagca gtagaaatca tcatgatccc caaaccacca
    16741 acttgaaata atatttccag cagtataata ccgttatcat tgaacacaga attgatatca
    16801 aaagtcgata gtccagtcac actcactgta ctgattgcca tgaacagcat gtccacaaaa
    16861 gaaacatggc tatctggttc gcgaaaaatc ggcatataaa atagaacgag tgataagacc
    16921 gtcatcaata tataataaaa aacgatgatt tggatcgatg agaaatgatt ggcagcaaac
    16981 ttctgtcctc gccgctgcag ccttcgaaag aataaatcaa catgcacagt actatcctcc
    17041 tgacaatagt cgtttctacc agtatacaag gggaattagg aatactcaaa gaaattatca
    17101 aacagataac aatacagctt tcgattaaaa tagaaatggc ttataataag gaaaatccta
    17161 gaaaaaagaa ggaggaggaa taacaaattg attaatgctc aacaaatcgt agaaaaaatg
    17221 aaaaatcaaa aaataaatta tgatacagtt ctgaaaaaaa tgatcatgca atgggagaaa
    17281 gaagaagtaa agcctaagat attaatacat acatgctgtg ctccttgcag tacgcacaca
    17341 ttggaattca tgtctcaata tgcagagatc acgttatttt tttcaaattc gaatattcat
    17401 cctaaaagtg agtatttgcg aagattacat gaacaaaaac gatttgtaga ccaatttaat
    17461 gagaaaactg accatcatgt tgcgctaatc gttgatgaat accgtccaaa tgaattcacc
    17521 aaaatggttt taacaaacca tcttgaaaac gaaaaagaag gtggtgctcg ctgcagtgca
    17581 tgcttcaata tgcgattaga tcttgttgcg caaaaagcgc aagaactagg atacgattac
    17641 tttggaagtg ccctgaccat ctcgccaaag aaaaatgcag ctttgatcaa tcagatcgga
    17701 atggatatcc aaaagattta cgggacacaa tacttgccaa gtgatttcaa gaaaaacaaa
    17761 ggctatgaac gctctttgca aatgtgcaaa gagtatgata tttatcgcca atgttattgt
    17821 ggttgcgtgt tcgctgccaa gcagcaagga tgcgatctga aagaaatcaa tcgggaagca
    17881 aaagcttact taaaaacatc aaccgtagtt tttgaggaaa attaaagaaa tctttcggaa
    17941 aaacgtaaga aaaaaataaa gaatttatgg tagaaataag gtagactgta taatttgtcc
    18001 gtagtaaagt aaggaaacaa gaataaattt caatgaagga agagataaga tgcgaaaagt
    18061 agcacctatt ttgaagccaa tcgctgtatt aacaatctcg acgattgtcg cgactactac
    18121 aggtattgtg aatagttcca gctaccgtgc accaagttca agttctagta ctgtagaaga
    18181 aacaacacca actacttcag tctcgacatc tgaaccaaca acaacggaaa gtttgaatga
    18241 aaccactacg acaagtacat gggacgatac aacaactagt tcgtcgtatg atgagacata
    18301 tacaactgac agctcaagcg aaacaagtaa tacggtacca gatggcactc catcggattc
    18361 aaccaatcca agtacaagtc cggatacaag tgctagctcc gaaacgatac caagcagtac
    18421 gactgtagat gataacacgc aaaatgatga tgcagcacgt acagcacagc aaaaccaaga
    18481 ttcaggaaat acccaaacgt ataaccagca agcaggtgat tctcaatgac atttttacta
    18541 gaccttatca acggggtgca taaattcctt ggctatttag acatcagtcc taaatatctt
    18601 aaccgtggat atacgatttt aagtgtgatt cccacgcttt atttattgag aattgtttat
    18661 ggactttggc aaaaccaaaa ctatcttcaa tttttcttat atggacttgc atttctagtg
    18721 cttgtttatt ttactgtttt aaatgtattt tattactttt tagataaaaa cacgaaagcc
    18781 gatgtcactc agctttttgt gaagtattta cctgatgaag cgttcaacat ccagacagaa
    18841 gaatcgatag ataatggaaa ttcgatcgat aaagtgaata cacaagaggt catggtttct
    18901 tatgacgaag attatcaatt gaaattagcc gagaatatgc ggtatttgat tggaaatggt
    18961 gagatcaaaa caaatgaact cggtcgcatg gatggatttc tggtcgatag aaatacattg
    19021 tatccttatt actatgtcaa gaaaacagga gagaaagagt atgttctaaa tattggacga
    19081 agctactcgg acatggaaaa agtagggacc attcgacttg actcaacaga agacacattg
    19141 gaacctgtcg gattgttcat cgtcggcggt gattttgtca aagacgggtt acgctatcat
    19201 gagccttatc gtctgaaatt gattgcaaaa aaggcacagt cgcaggaaga accagtggtt
    19261 tatagtcgta gccaaagaag aagacattca taatgtgatg aaacgccgtg tatcagtggt
    19321 tgcgataaaa acgtttattc ggaataaggg ggatgtgaca cgtcttttgt cacgttcctt
    19381 ttttatataa cgagaaagat aagacgaaca catcacttat tctttccttt catttataaa
    19441 cattgtagaa taaagaaagt acaacgaagt attgtgaaaa aacgtaataa ataacgagga
    19501 aaatacaagt aggaggtaag ctgtgaaaga atttttgaaa aattggggga ttttgatcct
    19561 tgtattagcg gctattttat tagctagagt gtacgtgttt acaccagtta ctgtaaacgg
    19621 gcattcgatg gaccctacat taagtgatgg acaacgattg atttcatcta agatctcaaa
    19681 ctatgaacga atggatatta ttactacaaa ggaaccgggg gatgaagaac ggatgattgt
    19741 taaacggatc atcggtatgc ctggagatac agtcaagatg gaaaatgacc agttgaccat
    19801 taatggcaaa aagtatgatg aaccttatct ggatgagttc aaaaaagaat tttcagaaga
    19861 taagttacaa ggagaatatg cttatagctc aggattccaa gcacaagcag aaagttcatc
    19921 cacctttacg agtgactttg aatatactgt gccaaaagga aagtatcttg ttctaggcga
    19981 taaccgcttg atttcaaaag acagccgaat gtttgggtta gtcgacaaag atatgatcca
    20041 aggaaaagtt gttttccgtt attggcctct ttcagaaatt aaaatcatgt gatgaaaaaa
    20101 taaaaagaag ctgtgacatg ttttttgtca cagcttcttt ttccgaaaat tccttcttat
    20161 ttcttggagt tgctcacttt ttttgcaatc tcattttaag ctttttggct gatggataag
    20221 aaaagcgaag cgtttttgtt cataaatagc ttttccccac gatggaaggt atgactagta
    20281 gcagagacaa agcccgtttt ttccaatcga ttattcaatt cttcttggct aaagccatta
    20341 tggactttcg gatgactaat ttgttcattc ttgtcaaagt ctacgatgat caattgaccg
    20401 ccaggagcta aaatttcata aagttttgta agaatattct ccgtatcagg aatgtgtaaa
    20461 agtacaagtg atagaaggat gagattagct tggacagcag gaaattcgac agaagcatca
    20521 gcgtggatcg ttcggacatt tttcagatct gctgcttgga tcttttcttc tgccattttc
    20581 agcatagttt ctgaagcatc ggcaatgatc agttctttga atctttccgc aagcggtaaa
    20641 ctaacaagcc cagttccgcc tccgtaatcc agcaggactt tttggtggct gtcgcgcggt
    20701 agataagtag ttagttcttc gcgaataatc ttggctaatg cttgacggtc aggggaatca
    20761 taatggttag cgatggaatc aaaaatattt tttgtcatca aatagctcct tacgtaaaac
    20821 tttgaaattt taacttatta tagcacgtga aaagaaagaa agcatgctat actcaattca
    20881 aaaagggaga atctagaaag aatatgccga tatcgtttgt aaaagataga gaggaaaaag
    20941 gaaagtgtgt ccgagaaata ttactagact tgcctgagtg gtttgggctt cctgaatcaa
    21001 cggaaaaata tatcgaggaa tcaagtaaac tacctttatg gtgtgaaaaa agaaaagaag
    21061 aatatttagg ctttatcact ctttcacaga caagtgaaga tactgccgaa atctattcta
    21121 ttgtatggga ataaaaaaag actatcatca ccaaggatta ggaacaaaac tttttaaaga
    21181 agcagaaggt tacgcagcaa aacattacaa atatttgcaa gtgaagactg tagacgaggg
    21241 gcactactct atatatgatc aaactatttg tttctacgaa tcattaggat tttcaaggtt
    21301 ggaagttttt ccaaatttat gggatgaatg gaacccatgt ttagttcttg tcaaaaaatt
    21361 ggaacaaaag tgagcaatag atttaatact aatctgtgct tgatgaaaat tctcactgta
    21421 aaaaatgctg aaaatcaagt ataataccat aggaacaaac tggaaggtga aaaagagcat
    21481 ggcacaacta ttttttaagt atggcgcaat gaatagcggc aagacgatcg agatcctaaa
    21541 agtcgcacat aattatgaag aacaaaataa gccagttgtc ttaatgacaa gcggtattga
    21601 tacaagagat ggtttaggcg ttgtttcaag ccgaatcgga ctgaaacgta atgcgatgcc
    21661 gatatttgaa gaaacagacg tatttgacgt aattcagaaa atggaaagca aaccctattg
    21721 tgtgctgatc gatgaagcgc aattcttaaa aaagaaacat gtcattgctt ttacgagaat
    21781 tgtggacgaa ttggatattc cggttatggc atttggtttg aaaaatgatt ttcgaaacga
    21841 gttatttgaa ggatcaaaat atttattgct ttatgcagac aaaatcgaag aaatgaaaac
    21901 aatctgttgg ttctgccaca aaaaagcaat catgaattta cattatattg atggaaaccc
    21961 tgtttatgaa ggcgatcaag tgcaaattgg cggaaatgaa gcttattacc ccgtttgtcg
    22021 aaaacattac tttcacccat caatgattac agaaggagaa taagcatgta cgatcaatta
    22081 caagcaattg aagaccgtta tgaagagctt ggagaacttt taagtgaccc agaagtgatt
    22141 tctgatacaa agcgttttat gcagctttca aaagaagaag caaacacgcg cgaaacagtc
    22201 gaagtttatc gtcgttataa agaagtagta caaggaatca ccgatacaga agaactgctt
    22261 ggtgaaaaac tggatgatga aatggctgaa ctagcaaaag aagaattatc tgaattaaaa
    22321 aaagaaaaag aagtcttaga agaaaaaatc aagatcttgc ttttaccaaa agatccaaat
    22381 gacgataaaa atatcatcat ggaaatccgt ggagcagcag gcggagacga agcagcatta
    22441 ttcgctggcg atcttttcaa tatgtaccaa aagtatgctg aagcacaagg ctggaaaaca
    22501 gaagtgatgg aagctaacgt taccggaatc ggtggatata aagaagtgat catgatgatc
    22561 accggagaca acgtatattc gaaattaaaa tatgaaagtg gtgcgcaccg cgtccagcgt
    22621 gttccttcaa ctgaatcgca agggcgtatc catacttcca ccgcaacagt tgtcgtgatg
    22681 ccagaagctg aagaagtaga gatcgatatt gaagacaaag atatccgtac agatatctat
    22741 catgcttcag gtgctggcgg acagcacgtc aataaaacag catctgctgt acgtttgact
    22801 cacttaccaa cagggatcgt tgttgctatg caagatgaac gttctcaaat caagaaccgt
    22861 gaaaaagcaa tgaaaatctt gcgtgctcgt gtgtacgata agatccagca agaagcacaa
    22921 agcgagtatg atgccaatcg aaaatcagca gtcggaactg gggatcgttc agaacggatc
    22981 cggacgtata atttcccgca aaatcgtgtg actgatcatc gtatcggatt aactatccaa
    23041 aaattagatc agattttagc agggaaatta gatgagatcg ttgatgcatt gatcatgtat
    23101 gatcaaacat caaaactgga agctatgcaa aatgggtaaa atgacctatc gagaagtcct
    23161 gtcacgggct tcttcttttt tagaggatca tgggaaagaa gcatatagta tccaattttt
    23221 atttttggaa cgaaaacaat ggaaaaaact tgattggctc cttcatatga acgaagagat
    23281 ttcagaagaa gaacagcggt tgatcgaaac cgatctgcga cttctgcttg cagatcatcc
    23341 acctcagtat ttgctaggat atgcagattt ttatgatcat cggttaaagg taactgaagc
    23401 aacactgatc cctcgaccag aaacagagga gttagtcgaa tggtgtttag acgagacgcc
    23461 tggtgtaccg ttagaagtga tagatattgg gacagggact ggagcaattg ctattagttt
    23521 gaaagcagca agaaaaaatt ggcatatatc tgctgtggat ctttcagaag aagcattaga
    23581 agtagcgaaa gaaaatgctc agaaagaagg aacgaaaatc agtttttatc atggagatac
    23641 attaggacca gtaatggatc agcaatttga tgtgattatt tctaatccgc cgtacatcag
    23701 tcggaatgaa tgggaactga tggatgaaag tgtccggtca ttcgaaccta aaatggccct
    23761 ttttgctgag aacgatggat tagctattta tgaaaaaata gcagttgaag caccaagcgt
    23821 actaaaatca aatggaaaga tttttttaga aatcggattt cgccaaggtg aagcagtaaa
    23881 aaaaatattt cagcaagcat ttccagataa aaaagtggcg ataaaaaagg atctttttgg
    23941 caatgaacga atgatccaag tttatggttg aagttgtgga tgaaaaattt tttatttgtt
    24001 tttttattat aaaaaaacac ttatttgaca aggatgtaaa cgactttttg gtttatcaac
    24061 aagtttatcc actattgaca aatagattat ccacaaacag tggaaaactt gaggataaac
    24121 tcagacaaaa taagaatgta tgaaaaaggg gttgtggata aactgtggaa acgaaacgat
    24181 atgatgaaac agaattgcaa gaggcagccg aagcactgaa agaaggagaa cttgttgctt
    24241 ttcctaccga aacagtatac ggattgggcg ccaacgctct tttaccaaac gctgtcaaga
    24301 aagtgttttc tgtaaaaggg agaccgcaag ataatccatt aatcgtccat gtcgcctctt
    24361 ttgaacaagt aaaagagtat gtggacaact ttcatccggc agcagagaag attgtgaaga
    24421 acttctggcc tggaccatta actttgatat ttaagatcaa aaaagacact ttgccctcag
    24481 ttgtgacggg aggattatcc acagctgcat ttcgtatacc ggataacaaa aaaacactag
    24541 aagtaatcga actttccggt gttccgcttg tcggtccaag tgcaaatacg tcgggaaaac
    24601 caagtccaac gactgcagat catgtatatc atgatctaca aggaaaaatt accgggatta
    24661 ttgatgatgg agctacgcga attggtgtag aatccactgt tcttgattta agtgatccaa
    24721 cagccatgcc tatgatttta cgaccaggtg cagtaacgaa agaacaaatc gaagcagtca
    24781 tcgaatctcc tgttgctatt gatcagcatc ttgtaaaaga aaatgaaaca ccaaaagccc
    24841 ctgggatgaa atacaaacac tactcacccg atacacgtgt tctgatggtc agagatggcg
    24901 attggtcaac tgcagttcaa tgggcaaaaa ataagaagat ccgtgcaggt gtcatagcaa
    24961 gccccgaaat tgcggatcaa gtgcggacag atactgcagc tgtttatatg tataacgaca
    25021 actcagtaga agcggcggct aaaggattgt ttgcaggtct aagaggatta gatgaaccaa
    25081 cacttggatt agatttgatt tttgtacaag tgtatccaga aactggattg gggaatgctt
    25141 acatgaatcg cttgaaaaaa gcagcaggac aaaattattt tgaaaaatga tcaattggtt
    25201 attgtcgcat tcttttagta tgatacaatg ctatcaagta aatcttctat cagtgaacga
    25261 aaaactaagc gtaactaatc gtaatagaag agatacgaat atttggcaat ttgtttttga
    25321 tgggaaaaat aaaattgtag gaaaataaaa aggagctgtt gtttgtggta gattacaaaa
    25381 cgtttgaccc tgatctttgg gctgcgatcg ccaaagaaga agaaagacaa gaacataatt
    25441 tggagctgat tgcctctgaa aactttgtct ccgaagcagt gatggctgca caaggaagta
    25501 tcttgacaaa taaatacgca gaaggttatc cagggcatcg ctattatggt gggtgtgaat
    25561 ttgtcgatat cgttgaaaat ttagcgattg accgtgcaaa agaattattc ggagcgaaat
    25621 ttgcgaacgt ccagcctcac tccggctctc aagccaacac ggctgcttat ctcgctttgg
    25681 tagaaccagg ggacacgatc ttaggaatgg atctttccgc aggcggacat ctaacacatg
    25741 gatcaccagt caacttcagt ggcaaaacct atcactttgt tgcttacggt gttgatccga
    25801 caacagaagt tattgattat aatgtggtac gtattttagc tagaaaacat caaccaaaat
    25861 tgatcgtagc aggtgccagt gcttacggac gaacgatcga ctttgcgaaa ttcagagaaa
    25921 tcgcagatga agtaggcgca aaactgatgg tagatatggc acatatcgca ggattagtag
    25981 cagcaggact tcatcctaat ccagttcctt atgcagatat cacaacgact accactcata
    26041 aaacattgcg tggccctcga ggcggcatga ttttgacaaa cgatgaagca ttagcaaaaa
    26101 aaataaatag tgcggtattt cctggtattc aaggcggccc tctagagcat gtgattgcgg
    26161 gtaaagcagt agcattcaaa gaagcattag atcctgcatt taaagaatat agtgaacaaa
    26221 tcatcgccaa tgcaaaagca atggtcaaag ttttcaatca agcaattgga accagagtca
    26281 tttcaggggc gacagataat catctgatgt tgatcgatgt tcgcgagtta ggtataaatg
    26341 gaaaagaagc agagagcatt ttggacagtg tgaatatcac tgtaaataaa aactcgattc
    26401 cttttgaaac actaagtcca ttcaaaacaa gcggtattcg aatcggcacg ccagcaatca
    26461 ctactcgcgg gttcaaagaa gaagatgctg taaaagtggc tgaactagtt gtcaaagcat
    26521 tacaagcaaa agatgataac gctcaactag acgaagtcaa aacaggtgta cgcgaattaa
    26581 ctgaaaaatt tccgttgcat aaaaaataaa aatttaaatt ccattagtaa acagaaaagt
    26641 tcaatgttgg gcttttctgt ttattttttg ggaaaacgga ttctttgaca atatcggtga
    26701 tgctctctag tatatgaggt gtaattttag tcttcacact aaatctcagg gaggaaaagt
    26761 catgactgat cgtatctcgt tagaaaagga agcagtagca ttcagtgatg caaatgcgcc
    26821 tcatccgcgt atctatgaac tagcaccgga agaaggaaga gttttactag aagaagtaca
    26881 agaatcacct gtgaacaaat attccgtcga tattgaagac ttgatggtgg atactggcga
    26941 atggggaaaa ctcaatattc gtctcgtacg accagaaggg aatatggata aattaccagt
    27001 tattttttat attcatggag caggctgggt ttttggcagt gctcaaaccc atgataaatt
    27061 gatcagagaa cttgcggttc gtacgaattc cgtcgttgtc tttccagaat atagtcgatc
    27121 accagaagca aaatatccaa cagcaattga acaaaattat gctgtgttgc aacagctgaa
    27181 taaattagcc aagtcaaaaa atctggatct cgagaatcta atcgttgcag gagattcagt
    27241 aggtggtaac atggctacag tcatgacgat cttaacaaaa gaacgaaagg gcctcccaat
    27301 atccaagcaa ttgctgtatt atccagtcac aaacgctgat tttgatacag attcctatca
    27361 tgaattcgga gaaaactatt ttttgacaag agaaggaatg atgtggtttt gggaccaata
    27421 tacgacgaat gaagaagagc gtgctgaaat cacagcttcg cctttgagag caaatataaa
    27481 tgaactaaat gatcttcctt cagcaatgat tttaacggga gaagcggatg tattaagaga
    27541 tgaaggtgag gcttatgcgc gcaaacttcg tgatgcaggg gttcctgtta cacaagtacg
    27601 cttccaagga atcatccatg attttgtcat ggtaaatgct atggatcaga caaaggcaac
    27661 gcgtgcagca atggcattat ctacttcttg gatcaagaaa aaagaataag ctttgcttgt
    27721 ccgcaggaaa acttcttttc aaaaacggat tttcttagga aaaatggcga gtttgactag
    27781 tcaatcactt gattttgttt tacaattaaa aagaaacgac agaacaaagg agagcttaaa
    27841 aaatgggcaa atttcaagtg atcgatcatc cattgattca acacaaacta actattattc
    27901 gggacaaaaa ctgcggaaca aaggtttttc gtgaagtagt cgatgaaatc gctatgttga
    27961 tggcatatga agtatcacgg gatatgccat tggaagacat cgtgatcgaa acaccgatca
    28021 ctgaaacaac acaaaaaacg ttgtctggta aaaaagtagc gattattcca atcttgcgcg
    28081 caggtattgg aatggttgac ggtatcttgg aattgattcc agctgctaaa gtgggacata
    28141 tcggtcttta tcgagatgaa gaaacattgg aaccacatga atatttcgtc aaacttccag
    28201 aagacattga tgcacgtcag ttgtttgtag tagatccaat gttggcaaca ggcggatcag
    28261 caatcatggc aatcgatgca ttgaaagaac gtggcgcgtc gaacattaaa tttgtttgtt
    28321 tggtagcagc acctgaggga gtaaaagctt tgcaagaagc gcatcctgat attgacatct
    28381 atacagcttc tctagatgaa aaattgaatg aacatggtta tatcgttcct ggactagggg
    28441 atgcgggtga ccgtttgttt ggtacaaaat aagtaaatac gaaaaagggt gtgacacaac
    28501 ttttgtcaca tccttttttg gtcattttta acgtttgatg cagaacagct ttttcacagc
    28561 tttcgtttgc atataaaaaa accatcccaa aaatgcagag atggtcaaat tcaaatcttt
    28621 tttagttctt tgtagttaac gattactaca cccttacttt gcgtcatcaa gttcgcattt
    28681 tctgcagttt catcaatctc aacaacagcg gaatttttca attgctttga aatcgttcca
    28741 gtttgcgact tgccgtgaat tgtgaacatt acgtgagtat tgatcttgaa tttttcacca
    28801 atttccgatg gtacgatagt tgtatcaaat tttccaaaag ttgccatttt cataaaacct
    28861 gcctttcaca tcaattatac cacgttttat gaaaaatatc atgaaattaa actaattttc
    28921 catcggactc tttacaatca gccaaataga gtatataatt tcttttggat aaggccagct
    28981 tagctcagtt ggtagagcaa cgcactcgta acgcgtaggt cgtaggttcg atccctatag
    29041 ctggcattaa aagagaagac agatatatca aggattttta atccattgat acatctgtct
    29101 ttttgtttaa aggaaataaa gtatgctttc ttagcaatag agcatgtatt atttctataa
    29161 aaatttagga gaataaaacg actagcaata tagctgtgaa atcaaatatc acatgaactg
    29221 cccaacttgt aatgatcgag ttgcttttaa tggcagcttg gttgaataac aatcttgcca
    29281 ccccttgtat caaaaggatg tgtgctacag tgtgtaaaat atttccatta tcataagtac
    29341 tgtaatggat aaatccaaag atgattgctg acaagaaact agcgacagga atgctgaact
    29401 tactagaaaa aatagcgaga aaatacactg agaacaattc ttctccaagt atcatgatag
    29461 gcaaaataat aaataataac gggctgaaag cctcatttat tggattgcct gttaaacccc
    29521 atttcaaaat ttgttgcaaa aataccgaga ctgcggcact gattactaca ttgataaaaa
    29581 aatatttggc tatatttttg acaggagcga caggtttcga gaacattttt ttcgtatctt
    29641 ttttaccaaa aacaagtgta aatgctataa atccagtgat agttaaagaa aaaatgctaa
    29701 taatggatgg cgcaaatagc aaccctaaca aaccgagtac aacgacaata gtgcccaata
    29761 tccctttttt aagttctatt ttttttccta aaatgttatt catgataata actcctacta
    29821 ttcagttttt gaagatagag tcagtataca tgaagagtta aaataggcaa tgttttttta
    29881 tggatatttg tattgctaaa aatttatctt atatattcat tttgattata ctacttttat
    29941 ttgtaaaaat tttctagcaa aaccaagtgc ttgcaactga ttaagcgtac aattttttag
    30001 atgttccgag cgtacttcaa tcatttcaaa ggagttgcta cttaaatcga tattttttaa
    30061 atcagtatca aaaaagctag cattttctaa atcattagta gaaaacatta ttttagtgag
    30121 ggaacagtct acaaaatctg cgcttgataa gacagagtgc tcaaaggtac tttcttttat
    30181 gtttgaacca gataaattta catagtccat tttacaattc tcgaataacg tgttcccaat
    30241 ataactttcc atcaatttag tacctgttaa tttacaattg acaaatttca cgcgatgcaa
    30301 catgcttttt gtcaatatac agcctgaaaa atcacagttt tcgaatatac agtcggtaat
    30361 ctccattctt tgaaaatcat tggaagcaaa aaatgcatac ttgaaaatag atttgtaaat
    30421 aattggatgg cagattattt ggttttcgat gacctctttt tgtgaaagga tattatacag
    30481 ttcaaaatct tcgttttcat agatatgaaa aaaggatcct ttattcaagc ttgcttcatt
    30541 aactttagga aaaatcatat ttaattacct ccattttcat aaagtcatat taacaaaagc
    30601 taaaccaact tacattagca gaaaatgaga taggttttcg cctttttcga taacgatagt
    30661 gtcgctatct cttgaaacgt tcttagaatt gccctgagag acacaaatta ggcgtaattc
    30721 taataaaaat cttagaataa aaatttttac tgatttaatt tagaatgttt ttaaaataaa
    30781 aatcgttgcc ataacagtgt attgaaaggt tttaacaatc cagtaatgat ttaaaaaaat
    30841 ctagatgtag cgtatacttt gtttatagaa tgtagtggga agaaatatat ttttgggagg
    30901 aaatcgtgat gtctaaaagt gaagaaatag aaaacatggt actaaaatta tatgatctcg
    30961 ttttaaatcc agagatcaaa gaaaaggaac gtatactttt gatagacgca aagacaggct
    31021 tagaaaaagg tcagtattat cctaaagtca taaataattt ggaacgtagt ttacgtccgt
    31081 tagcaatcag gggagaattg tctaaaccag tatcaccgtt ttatatggag atatcaacaa
    31141 taggaaaatt tgaaaaagaa ttaggtcgag gaatggcttc ggcaccgatt acttttgggc
    31201 atttataaaa acgagttatt catctcaatg aattaaaaag tatagtcaat ctcataatgc
    31261 aggctaggtt catttatttg aacagagtct gtattttttt attctaaata aagaaacaga
    31321 tgtttcttta tattgtattt tatcctttaa ttaacacaaa aacagctagt ataaactaat
    31381 aatgttaatt aaaagtgtat tttatgaaac tatcgttatt tattaatgtt gcatttttgt
    31441 aagttaaaaa ttggaaatat agattgaaaa aatgaaagca ataagtaaaa taaaatgaat
    31501 atatgcgaaa aaataattta cattaggaaa aaccttgtaa aaacagtttt ttggcatatt
    31561 tattttttat aaatatttgt aaaataacta tcaatttttt agttaaaaga ttggaaatga
    31621 gtgggggagg aattgaagaa aggatttttg agagatgata tagtaggttt tatttttagt
    31681 taaaataggg gagatagaaa aaatgaaaaa gtgtagaaat attattttat caatgttatt
    31741 tattattact aatataacat cactgattcc tgttcatgtc tatgcggatg caggcagaga
    31801 tatcagcagt aatgtcactt cgttgaccgt ggacccaacg aatattacag atggaggaaa
    31861 cataaaagta aaattctcgt ttgacgaaaa aaaacaaaat atccaaccag gtgattatct
    31921 ttggatcaat tggccaagtg aaggaaatat tcgcggtgaa ggattccaaa aagaaatccc
    31981 attaatgatt gaaaataaaa atgttggaac tttaacggtc agaaaagatt ctgcgcaggt
    32041 tgtgtttaat gaaaatatta aaaacctaga ctcagtagaa ggttgggggc aatttgagat
    32101 acaggccaga aacgtaaccg ataccggtga agaaaatacg ggaccattca ccgtgacgag
    32161 cggtgataaa acagctactg taaatgtgac aaagccagct tctggttctt cttctagcgt
    32221 tttttattat aaaacaggag acatgctacc tgaagatact aagcacattc gatggttttt
    32281 gaatattaac aataacggca cttatgttga acaaccagtt aaaatcagtg atgaaatcca
    32341 aagtggacaa agattagatc caagtacgtt tgaaattaat caaatacact taggtgaaca
    32401 gaaggtttat agaggagaag agggaatcca acaattttta caagatttcc ccagcgctac
    32461 atttaatttt tcagtaactg ataattacat tgaaataact ataccaaaaa actttgtcaa
    32521 tcttaggaaa ataatggtga gttataagac gatcatagaa aacccagaac aaataaattt
    32581 tgagaatcat tcggaagctt ggtttaaaga atttaataaa cctgctgttg atggagaaag
    32641 tttcaatcat acagttaaaa atatttcagc ttctggtgga gtcaacggta cagttagagg
    32701 cgaattgaag attttcaaat atatcaatga cacagagatt ggtatcccaa atgtgacttt
    32761 cgaattaaga cgagcagatg aacagcctat tcaaggacag tctagtatcc tgttgacttc
    32821 gaatgaacag ggagaagcta gtatcaaagg cctgcaagta ggagactatg ttgtaaaaga
    32881 aaaagaagca cccaattgga ttgattttga tccactaagt tcaaatgaac tcaaattctc
    32941 cataaacgaa aatgatacag agggagtcag tttacctatt tataacaaga aaaaggttac
    33001 gaacatcaca gcaactaaaa tttggaacgg tggaaccact ccaagaccga gtatttattt
    33061 taaattattc agagcaagta caaacaattg ggaaccagtt cctgatgcag aaacaaaacg
    33121 attagacaat ggaataactt cagtgacttg ggaagatatc caacagtatg atgatagtgg
    33181 aaacgaatat acgtttaaag tgcaagaagt agatcaaaac ggaaatgatt atgtcccttc
    33241 aggttatcaa aaaattgaaa acgggctagt cgttacaaat gagaataaag aagtcatttc
    33301 agtctcagga caaaaaacat gggaagacaa tgagaaccaa gacggcaaac gaccgacgac
    33361 gatcacagta aatctattag ccgacggtca accgatccaa cacaaagaag taagtgaaaa
    33421 agatgattgg cgctatcgtt tcacaaatct gccaaaatac agagacgggc aagaaatcat
    33481 ctatactgta acggaagaca acgtgcccga atacagtacg acgattgacg ggtataacat
    33541 caaaaacagt tatacaccgg aaaaaacaaa tatatctata ccaggtagcc atataacacc
    33601 gtggaaaaat tttccagaga agacagagag agagaaaatt agaaatctat tctctcaaaa
    33661 attgcaaata tttgctcata caggagaatc cagcgaacaa agaagcaatt atattagtaa
    33721 gagagctgta cctaaacaga taacaaataa atcaaaaagc attcttccta aaacaagtag
    33781 taaaaaatct agttttgctg taatcattgg tttattgata gtaactatct gtgctgggat
    33841 attcttacaa aaatataaaa aagtttagtt agaatataat attttttgtt aaatatgact
    33901 gaaatcaaaa gaataaaatt ccgatttata atcgaaaaaa gaagcatttt tatatatgct
    33961 tcttttttcg tagaatcaat aaatatggat ttggctaact atggaagaat actcgtatta
    34021 gttttcagcg ttcatactag ttgttacatt gtttgattgg ccaaaagaat ttctgatata
    34081 ttttgtgcta aataaagaaa aagacgcttg aattgggaaa taagaaaact actctatcat
    34141 tacaaatttt cataaactgg cagatgttct aagtaaacaa gtatcttttc aaagtcggaa
    34201 aaatccataa tcaacgtagt ggtattgata tttggatgaa agcctacggt atcttgacga
    34261 tcaatagcag catcgatcac gacttggatt tttttctctt tgtcgtgcat caatgctaat
    34321 ggagtcactg tacctgctgg aacgcctagg aggtcaggaa gctgttcttc agaaacaaaa
    34381 gataagcgct tttcttttaa ttgttcagct agttgtttta aatcggcgtg cttttcatca
    34441 ggcaagatga caagatagat ttgttttcct tttcttgatt tcaataatag attttttact
    34501 tgcggaccag gtagggaaaa agggacattt ttgactgacg taatcgcagg atgatcgact
    34561 ttttgaaaag agatacccag tttttttagt agttcataag cttcttgttc ggttgcacga
    34621 ctcatcgcgc atcaccctcc tttttttatt agtgtatcat aaaaaagagt tgacaaatga
    34681 aaaataatca tgtaaattac acgtaaagtt aaaacaaatg acatcgaaaa gaagagtagc
    34741 cgttaggtgt ttttagagag tctctggttg gtggaaagag gcagcagtta accgtgaaac
    34801 acatcttgga gtcgattttc tgaagcgtat agtaggaaaa tccggggagc ccgttacagc
    34861 tgcggtcgtt gctagatgac cagataaggc tggcttttcc gtgaggagtc ggtaaaaaaa
    34921 ggtggaacca cgtgattaac gtcctttgat cttcggatca aaggactttt tgttttcatc
    34981 tgaacggcaa cgactgtgtg aggttttttc tgcaaggaaa aaataaacag aggtggaacc
    35041 acgtataata cgtcctcttg gcatttgcca agaggatttt tttattttaa aggaggaaaa
    35101 gaagatggag tatttgattg aagttatgcc gcaattatta gtaggagcac tcacaacatt
    35161 gaaattattt ttatttacct tgattggctc attaccatta gggattgttt tagcattagg
    35221 attgtctttg cattttaagc cgctacgcta cttgcttcaa acatatgtat gggttatgcg
    35281 gggaacaccg ctgttgttgc aactgatttt tattttttat gggttgccga cgattgggat
    35341 cgtttttgat cgtttcgaag cagcactcat tgcctttatc ttaaactatg cagcttattt
    35401 tgcagagatc tttcgtggag ggatacaatc gattcctcaa ggtcaatatg aagcagcgca
    35461 agttttgcaa ttaagtccct ttcaaacggt gacgaaaatc gtcatcccac aagtgacaaa
    35521 gattgtttta ccttctgtag ggaatgaagt tatcaacttg gtcaaagatt cttccttgat
    35581 ctatatatta ggtttaggtg acgtaatgcg tgctgggaaa atcgcgatgg aacgcgacgc
    35641 gactttactg cctttgctag gcgtggcaat catctatctt acattgactg gtattctaac
    35701 aatcgtaatg aaacaagttg aaggaaaatt aaattattac cgctaactga taggaggaag
    35761 aaatatgatt gaattgaaga aagtgaacaa agcatttggt gaaaaaaaaa taatcaaaga
    35821 tttgaatcta atcattccag atgggaaaac attagctatc gtaggtcctt ccggtggcgg
    35881 taaaaccacg cttttacgta tcttggctgg cttagaaagt tctgacagcg gagaatttta
    35941 tttagacggt gtaccgttca acccattcga gcttaaaatg aaagaacaag ttgtaggagt
    36001 cgtattccaa gacttccagc tatttccaca tctgaatatt tttgaaaaca ttaccttagc
    36061 accaaaaatg gtattgaaac agacaaaaga agaatacacc aaaaaagcag aagaattagc
    36121 ggcttcatta gggattagtg atttattgaa tatctatcct tatcagcttt ctggcggaca
    36181 aaaacagcgg gtagcgattg ccagagcatt ggctatgaat ccacgggtat tggcttatga
    36241 tgaaccaaca agtgctttag atcctgaatt acgccagcaa gtcgagcgtt tgatcctatc
    36301 attgaaagaa gatggggtaa cccaaattat tgtcacccat gatttagtat ttgcagaaaa
    36361 tgtcggtgat caattactaa aagtttcccc aattcattaa gaaaggagaa gatagagatg
    36421 aaaaaaacca gttttctatt cgctttgatc gcaggtcttc ttctttttgc aggctgttcg
    36481 aacaaaaaaa cttcagcaga taattgggcg actatcaacg aagagaaaaa aatcgttata
    36541 ggtttagacg atacatttgt tcctatggga tttagagata aagacgggaa gctcacagga
    36601 tttgatattg atttagccaa agcagtcttt gaacaatatg ggattacggt agactttcag
    36661 cccattgact ggtcaatgaa ggaatttgaa ctgaacaatg gaacgatcga tttgatatgg
    36721 aacggctatt ccaaaacgcc ggcaagagaa aagaaagtgc aatttacaaa accttatatg
    36781 gaaaacgatc aagtgctgat cacacctaaa gaatcaggaa tcacaaactt tggacaaatg
    36841 aataataaga gattaggtgc gcaaaatggg tcatctggtt atgatgtatt tacgaaacaa
    36901 ccagaagtat taaaagagat agtcaaaaac caagatgctg ttttgtatga tagttttaat
    36961 gaagcattga tcgatctgaa aagcagacgg gtcgatggtc tgctgatgga taaagtatac
    37021 gcagactatt atttgaaaca aagaaacgaa ctagaagact tcaacataac aaaaggttct
    37081 tatgagagtg aggattttgc agttggtgca cgcaagtcag atagtacatt agtgaaaaaa
    37141 atcaatgaag ccctagatca actagagaaa acaggtgaat tccaaaaaat atccgataaa
    37201 tggtttggcg aagatgttac gccgagaaat taacaaatag gggactgtga caaaagacgt
    37261 gtcacagtcc ctttttccga ataaacagtg ttctcctgcc aactccttgt gactcggagc
    37321 tgaatggcag gctcacaacc tcttggtaaa cggtgttcaa aagccagttt ttcggtatta
    37381 agccaaattt atgcaaaaat gtagaaagcc attttcacaa aatttttctt actactcgaa
    37441 actaagcgac tttttcacaa cctcctttac atatttttat tttgttgtgg aaggggtttc
    37501 gtgttattct aaaagaggaa cgattgaagg ttataggaaa ggaagagatg aatggaacac
    37561 aaagaactta cctcatttcc aaaagatttt ctgtggggct ctgcttctgc agcgtatcaa
    37621 gtagaaggag cttggcaaga agatgggaaa ggagaatcag tctgggatcg atttgtacgg
    37681 atacctggaa agacatttaa aggaacgaac ggtgatttgg cagtagacca ctatcatcgt
    37741 tataaagaag atattgcttt aatgaaagaa caaggtctga aagcttaccg cttctctgta
    37801 gcttggacac ggatttttcc taatggacga ggagaagtca atcaagctgg gctcgctttt
    37861 tatgagagat tgatcgacga attgattgaa aacgatattg aacctatgtt gactttatat
    37921 cattgggatc ttccacaagc attgcaagat gaatataatg gttgggaatc acgtcagatc
    37981 attgaagatt tcactcatta tgcagaaaca ttatttgagg cattccgcgg gaaagtccat
    38041 tactggatta gtttgaatga acaaaatatc ttcacttcat taggttattt gttagcagca
    38101 catccgccag gtgtgacaga cccgaaacga atgtatgaag ttaaccatat tgctaattta
    38161 gcaaatgcat ctgtcatcaa taaatttcac gagttagaaa tgcctggaaa aatcggacca
    38221 agctttgcgt atacgccaaa ttatccaatt gacagtaatc ctgaaaatgt tctggctgct
    38281 gaaaatgcag aagacctgat ggccaattat tggatggatg tctatatgtg gggaaaatat
    38341 ccgattgctg ccatgaggtt cttagaagaa aaaggatggg caccaacgat tgaagctggc
    38401 gatgcagaat tgctagaatc tgctaaacca gattttcttg ggatcaatta ctatcaaacg
    38461 gcgacgaatg cattcaatcc attaaacgga gttggtgctg gaaaaatgaa tacgacagga
    38521 aaaaaaggtt catcagaaga aactggagtt cctggaatgt ataaaaaagt tgagaatcca
    38581 tttgtcgaac gaacaaattg ggactgggag attgacccag aaggattaag gatcggactg
    38641 cgtcgtatca caagccgtta ccgcatgccg gtactgatta cggaaaatgg cttaggagag
    38701 tatgacaagt tgacagaaga taaacaaatc catgacgact atcgaatcaa ttacttacaa
    38761 agccacgtca aagccatcaa agaagcaatt tcggatggag cagaagtcct aggctattgc
    38821 acctggtctt atacagattt gcttagctgg ctgaacggtt atcaaaagcg atatggattt
    38881 gtatatgttg atcaagacga aacacaagaa ggatctttgg aacgatataa gaaagacagt
    38941 tattactggt accaaaaact gattgaagaa aatggagaga acctttagtt cacatactta
    39001 aaacaagaag gtacggctgg atatggccgt gccttttctt gataaaatca tttagaaatt
    39061 aagaaaaact gtaaaataag ctgaaaaatg aaagaaagcg tcagttcttt caaacaaaac
    39121 tttagttccc ttactttttt ttgtataatg aaacttgatg aaatgaggga taataaatgg
    39181 cacaaaagaa acggcctact aaaaaaagga aaaagacgaa aaaaacgaaa aagcaaaaac
    39241 agcagcagga acaaatgctg acgattggtg ttgggtttgc cttgatttta tttgctgtct
    39301 tcggcttttt aaaattaggt tttttaggaa tattgattgc aaacggcttt cggatcattg
    39361 caggaaatac ttaccaaatc ctctgtcttt tattagccgt attaggattt tggattgtca
    39421 ttaagaatac agagttttca attggtaaaa accgacgctg gtttggcggc atactctttt
    39481 atttcggtat cttattattg ttacatgccc atttatttgg caaactccat actggggaac
    39541 caaatatcat ggggacgaca tgggatttgt tagccagtga tataaaacaa agtcaagtag
    39601 acaataacgt tggtggtggg atgattggag caatccttta tcattttact tacttcttga
    39661 ttgcacagcc tggaagttat ttagcagcag tgctattact ggcaggagga gcattcctgt
    39721 tcagtaatct ggaaggctat cagctgttaa atggtatcca atcaattgga gaacgagtac
    39781 aggaattatt ggaaggtgat cctgccaagc aagctcgaaa gcaagcggca aaggaagaga
    39841 gaatgaaaca gcgcgcagaa gcgaaagaag caaggcgctt agctgcaaaa gaagctgcag
    39901 aaaaagaagc tgcagaatat gagaagaaaa aagcggtgag ccagcaaagg gaaaatactg
    39961 ctgatgaatg gcaggaacca gaaaatacag agccggagca gctaagtttt gttccaatcg
    40021 acagttttca agaaaatatc catcctgcga atctagagaa accagtacca gatacaccaa
    40081 aacagaccaa tacagcagaa ggtttttcag atgagttgcc agaggatgac ggtacctcat
    40141 tagaatttga gattgaagca gaacaggaaa atcaagacta tgaattaccg acagttgatt
    40201 tgcttgattc tataccaact gtggatcaaa gtgatgagta taaaaaaatt gaaaaaaata
    40261 tcggcgtatt agaacaaact ttccaaagtt tcggtgtcga tgccaaggtc gtcaaagcca
    40321 gcttaggacc atccgtcaca aaattcgaag tccagccggc tgtaggggta aaagtaagta
    40381 aaattgtgaa cttaacagat gatatcgcgc ttgctttagc ggcgaaagat gtccggatgg
    40441 aagcaccgat tccaggtaaa tcactgattg gtattgaagt accaaatgga aagatcagta
    40501 tggtttcctt tagagaaatc atcgaagcac aaccaaacca cccagataaa ttactagaag
    40561 tacctttagg acgtgatgtt tctggacggg tacaaacggc tgatctttcg aagatgcctc
    40621 acttactcgt ggctggttcg actggtagtg ggaagtcagt agcaattaat gggattatca
    40681 caagtatatt aatgcgtgct aagccccatg aagtaaaact gatgatgatc gatccgaaaa
    40741 tggttgaatt gaatatgtac aacggcatac ctcatctgtt gacgccggtc gtgacgaacc
    40801 ctagaaaagc agcgcaagcc ttacaaaaag tagtacaaga aatggaagaa cgatatgaaa
    40861 aattcgcggc aaccggagta agaaatattt ctggttacaa tgaatttgtt caacagaaaa
    40921 acttggaaaa cggaacgaaa catccaacct taccattcat cgtagtgatc gtagatgaat
    40981 tagctgattt gatgatggta gccagcaatg aagttgaaga tgcgattatt cgtttagcgc
    41041 aaatggctcg ggcagcgggg attcatatga tcctagctac tcaacgtcca agtgtggatg
    41101 ttatcacagg aatcatcaaa gcaaatgtac cttccagaat ggcatttgcg gtatcaagcg
    41161 gtacagattc tcggacgatc attgattcta atggggcaga aaagctctta gggcgaggag
    41221 atatgctatt cttgccaatg ggtgagaata aaccgatccg tgtgcaagga gcattcattt
    41281 ctgaccacga agtagaacgg gtcgttcaat ttgttacaga tcagcaagaa gctcattatg
    41341 aagagaaaat gatgccgaca gatgaagttg aaacgacagg tgctccagaa cagccacaag
    41401 atgagctgtt tgaagaagca aaagcacttg tcgttgaaat gcaaacggca agtatttccc
    41461 ttctccaaag aagatttcgg atcggttaca accgagcagc acgtttagta gatgaactgg
    41521 aagctcatgg agttgttggc ccgtcagaag gaagcaagcc tcggaaagta ttcattgagc
    41581 aggaagaaac atcagaaacg ttgccggacg ataatgacat gtaaaacttg tgtgatgaag
    41641 ggttgaaaaa cgaataacca ataaaaagac aaggcatgat gataccaaaa gaaaaaatgg
    41701 tttttttcca tcttttctaa tacggtatga tgtcttgtct ttttagtttt tgtgagatta
    41761 aagttctatt cgatttattt gtgaagtatt gcattaagga aatgaatcat gtatagtatg
    41821 tagcgaaact ttgagaaaaa gagggaaaat actttggaaa aacagtccat ttatggcttg
    41881 acgaatgaag aattaatcaa ttggtttata gaaaacggcg agaaaaaatt ccgagctgcc
    41941 caagtttggg aatggttgta tcaaaaacgt gtaagtaatt tcacagaaat gacgaatcta
    42001 tcgaaacaat tgatcgaaaa attatcggag cattttatca tcaatcctct aaaacagatg
    42061 gtcgtccaag aagcttcgga tggtacagta aaatatttgt ttgaactccc tgataaaaac
    42121 atgattgaga ctgtactgat gagacaagaa tatggcttgt cagtttgtgt gacgacacaa
    42181 gtaggatgta atatcggctg tacgttttgt gcaagcgggc tgctcaaaaa gaaccgtgat
    42241 ctgactgctg gagaaattgt tgcacagatc atgatggtcc aacattattt tgatgagcgc
    42301 aatcttggcg aacgcgtttc acatgtcgtt gtaatgggga tcggtgagcc atttgataat
    42361 tacgacaatg taatggactt tttgcatatc atcaatgatg ccaaaggatt ggcaattggt
    42421 gctcggcaca ttacagtttc cactagtgga ttagcacaca aaatcaagga attcgctaat
    42481 aacggcttgc aagtcaatct ggctatttcc ttgcacgcgc cgaataatga ggtgcgaaca
    42541 tcgatcatgc ggatcaaccg aagtttccca attgagaaat tgatggaagc tgtggatgag
    42601 tatttagaga aaacaaaccg tcgaatcacg tttgaatata tcatgttgaa tcaagtaaat
    42661 gatagaccag agcatgcgca acaattagca gatctattaa aagataagaa aaaattagca
    42721 tatgtcaact tgattccata taatccagtc agtgaacatg atcaatattc tcgcagtcca
    42781 aaagctgatg tattgagatt ttatgatgta ttgaagaaaa acggcgtaaa ctgtgtgata
    42841 cgtaaagaac atggtacaga tattgatgca gcttgcggac aattgcgaag caagcaaatg
    42901 aaaaaaacag ttgccaaata atcgtatcct actcgttttt tcaattacca taaaaggagc
    42961 agttttctta attaaggaac tgttcaactt aaataagaga aaagattcta tagcgaaagc
    43021 tataaagctt tttttgacaa gaaaaaccct catcaagcaa agaaatgtcc tcaatttgat
    43081 gacagcctgc ttgatggggg ttttagcaat tatttaactg attttcttat atccttgctg
    43141 gctttcttgt gtagcatgat aagtttctac gtcaagaatg gcctgtttaa atttttctcg
    43201 ggtgatttca taaggcacat gtttaatatc atttgttgcc atggctttat ctaagattgc
    43261 ttctaaatcg gtcgtgacat tgagattcat gtcggttaac gatactggaa gttggttctt
    43321 tttcataaac acatagattt tatcaaacaa atcgaactgc tgatccatca tcaataaggt
    43381 cagtacacca taactgacag atgctccgtg aaggtatccc tccatttttt ctagaacagt
    43441 cgtaccgtaa tagaatgagt gtgccatagc agaattatag ttattgttaa ctaaaacaga
    43501 aaccaatccg gtagtaacaa tgatatccga aacgacttgt tcaattgcat atgagggacg
    43561 atcgtcggca ctatctttca atgcttgcgt accataagtc aagagacgat caatgcacat
    43621 ttttccgatt tggattccta atctatccgt gtagcctaga tcatcgccac gagcagaaaa
    43681 agtagattcg acttctttgg ataaagcatc gccaatacct gcccataagt atttatcagg
    43741 tgcttcggca ataattgctg tatcaatgaa ggtgtgtaca ggtggttttg aaggaaagaa
    43801 gagtccgtcc attacgcctt cttcgttata gatgacacat accgcagtga cgggcgaaca
    43861 gttcgaacca atagtgggaa aggtgaagac tggtttagct agtttttcac caatgacttt
    43921 tacagtatcg caagcacggc caccaccgac tgcaaacagg acgtctgctt tttggacact
    43981 ttcatgctgg atcaactgtt caatatttcc ataagttgaa ttacccccgt accagagact
    44041 ggcgacaacc tctatttcct gtgtcaaagc tacttttatt ttgttttctg cctttgaaag
    44101 tgcagtcttc cctccaatca gcactacttt tgtcccatat tctttgacga ttccatttgc
    44161 tttttcatac gcatctgttc caatggtata atttggtaag aaaacacttt gactcatatt
    44221 catttccttc tttcttcgtt gaaacgggtt aaaattttaa agagtgatcg atctgactgc
    44281 ggctgtagat ggagtagagg tgaacctaag acaggataag tgatgaataa ggtggcatga
    44341 acaccttgaa atcatcaata agtatcctaa aaacgaacaa gtacaaaaac attgaaaacg
    44401 acaataatga atttttgttc ctcttagtat ttctaccgat tccctttgct tttttgtaat
    44461 tatctaaaaa ttctaaaaat taagattatt atatgaaaac aggttttatt tgtcaattca
    44521 taattggttt tttttaaagc aaaagataat ctatatcatg atgaacaaac agagtttttt
    44581 cctccaattg atggctttcc tgatctttcc tattttgaat aaagtgtttc tgtataattt
    44641 ttttatttta taagtaagat aaataatgaa agaatagaga tactgattgg tagctaaaga
    44701 tttttgagcg agaacatttc gttttttcaa aaggactgtt agaataagca agaacaaggg
    44761 ggaagacgta tgataatcag tgaatttgac cgtaataatc cagtattgaa agatcagctt
    44821 tctgatttac tgagactgac ttggccggaa gaatatggag acagctcggc agaagaagta
    44881 gaagaaatga tgaatccaga acgaatcgcg gtagcagcgg tagaccaaga tgagttagta
    44941 ggatttattg gtgcaatccc tcaatacggt atcacaggtt gggaattgca tccattagtt
    45001 gtagaaagct cccgacgaaa gaaccaaata ggtactcgat tagtcaatta cttagaaaaa
    45061 gaagtagctt ccagaggagg aatcacgatt tatttaggta cggatgattt agaccatgga
    45121 acaacgttaa gtcaaacgga cctgtatgag catacatttg ataaagtggc ttccatccag
    45181 aaccttcgtg aacatccgta tgaattctat gaaaaattag gttataaaat cgtaggtgtc
    45241 ttaccaaatg caaatggctg ggacaaaccg gatatttgga tggcaaaaac gattattcct
    45301 cgaccaaatt ctcaataata aaaagaggtt gtgacagaag taatcagctt caagaaataa
    45361 gaaggaattt acgaaaatta cttcttgaat ttttgtgaaa ttccaactta tttccgaaga
    45421 agctactttt ggaacaccgt gtac
//
LOCUS       DACKAC010000016        41210 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.016, whole genome shotgun sequence.
ACCESSION   DACKAC010000016 DACKAC010000000
VERSION     DACKAC010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41210
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.016"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(26..328)
                     /locus_tag="I0B46_000938"
     CDS             complement(26..328)
                     /locus_tag="I0B46_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAR2083256.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(353..2446)
                     /locus_tag="I0B46_000939"
     CDS             complement(353..2446)
                     /locus_tag="I0B46_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAR2083257.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(2935..3699)
                     /locus_tag="I0B46_000940"
     CDS             complement(2935..3699)
                     /locus_tag="I0B46_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAR2083258.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="I0B46_000941"
     CDS             complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="I0B46_000941"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAR2083259.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(4704..5780)
                     /locus_tag="I0B46_000942"
     CDS             complement(4704..5780)
                     /locus_tag="I0B46_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAR2083260.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(5804..6397)
                     /locus_tag="I0B46_000943"
     CDS             complement(5804..6397)
                     /locus_tag="I0B46_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAR2083261.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="I0B46_000944"
     CDS             complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="I0B46_000944"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAR2083262.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(8067..8366)
                     /locus_tag="I0B46_000945"
     CDS             complement(8067..8366)
                     /locus_tag="I0B46_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAR2083263.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(8468..10114)
                     /gene="mdcA"
                     /locus_tag="I0B46_000946"
     CDS             complement(8468..10114)
                     /gene="mdcA"
                     /locus_tag="I0B46_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HAR2083264.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(10121..11032)
                     /locus_tag="I0B46_000947"
     CDS             complement(10121..11032)
                     /locus_tag="I0B46_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAR2083265.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            11152..12009
                     /locus_tag="I0B46_000948"
     CDS             11152..12009
                     /locus_tag="I0B46_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAR2083266.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(12196..12558)
                     /locus_tag="I0B46_000949"
     CDS             complement(12196..12558)
                     /locus_tag="I0B46_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083267.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            12904..14343
                     /locus_tag="I0B46_000950"
     CDS             12904..14343
                     /locus_tag="I0B46_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAR2083268.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(14350..16902)
                     /locus_tag="I0B46_000951"
     CDS             complement(14350..16902)
                     /locus_tag="I0B46_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAR2083269.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(17016..18347)
                     /locus_tag="I0B46_000952"
     CDS             complement(17016..18347)
                     /locus_tag="I0B46_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAR2083270.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(18313..19791)
                     /locus_tag="I0B46_000953"
     CDS             complement(18313..19791)
                     /locus_tag="I0B46_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAR2083271.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMSKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(20096..21931)
                     /gene="tdc"
                     /locus_tag="I0B46_000954"
     CDS             complement(20096..21931)
                     /gene="tdc"
                     /locus_tag="I0B46_000954"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAR2083272.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            22401..23828
                     /locus_tag="I0B46_000955"
     CDS             22401..23828
                     /locus_tag="I0B46_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAR2083273.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(23920..24285)
                     /locus_tag="I0B46_000956"
     CDS             complement(23920..24285)
                     /locus_tag="I0B46_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAR2083274.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(24282..24647)
                     /locus_tag="I0B46_000957"
     CDS             complement(24282..24647)
                     /locus_tag="I0B46_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAR2083275.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(24839..25087)
                     /locus_tag="I0B46_000958"
     CDS             complement(24839..25087)
                     /locus_tag="I0B46_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083276.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            25086..25214
                     /locus_tag="I0B46_000959"
     CDS             25086..25214
                     /locus_tag="I0B46_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAR2083277.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            25271..26965
                     /locus_tag="I0B46_000960"
     CDS             25271..26965
                     /locus_tag="I0B46_000960"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAR2083278.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            26969..27088
                     /locus_tag="I0B46_000961"
                     /pseudo
     CDS             26969..27088
                     /locus_tag="I0B46_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            27170..27361
                     /locus_tag="I0B46_000962"
     CDS             27170..27361
                     /locus_tag="I0B46_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083279.1"
                     /translation="MDLLPQMQAFEHHLPRGYEKAALHQVIRAAKLKQLLMFLTWLDL
                     LPQMQAFEHRLPRGYEKGV"
     gene            27739..28872
                     /locus_tag="I0B46_000963"
     CDS             27739..28872
                     /locus_tag="I0B46_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAR2083280.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            28903..29223
                     /locus_tag="I0B46_000964"
     CDS             28903..29223
                     /locus_tag="I0B46_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083281.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(29252..29716)
                     /locus_tag="I0B46_000965"
     CDS             complement(29252..29716)
                     /locus_tag="I0B46_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAR2083282.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(29847..31104)
                     /locus_tag="I0B46_000966"
                     /pseudo
     CDS             complement(29847..31104)
                     /locus_tag="I0B46_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
     gene            complement(31133..31450)
                     /locus_tag="I0B46_000967"
     CDS             complement(31133..31450)
                     /locus_tag="I0B46_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAR2083283.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(31476..31796)
                     /locus_tag="I0B46_000968"
     CDS             complement(31476..31796)
                     /locus_tag="I0B46_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAR2083284.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(32260..33675)
                     /locus_tag="I0B46_000969"
     CDS             complement(32260..33675)
                     /locus_tag="I0B46_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAR2083285.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(33692..35008)
                     /locus_tag="I0B46_000970"
     CDS             complement(33692..35008)
                     /locus_tag="I0B46_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAR2083286.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(35158..35856)
                     /locus_tag="I0B46_000971"
     CDS             complement(35158..35856)
                     /locus_tag="I0B46_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAR2083287.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(36145..37134)
                     /locus_tag="I0B46_000972"
     CDS             complement(36145..37134)
                     /locus_tag="I0B46_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAR2083288.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(37214..38047)
                     /gene="msrA"
                     /locus_tag="I0B46_000973"
     CDS             complement(37214..38047)
                     /gene="msrA"
                     /locus_tag="I0B46_000973"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAR2083289.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQGERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(38222..39856)
                     /locus_tag="I0B46_000974"
     CDS             complement(38222..39856)
                     /locus_tag="I0B46_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083290.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(39843..41177)
                     /locus_tag="I0B46_000975"
     CDS             complement(39843..41177)
                     /locus_tag="I0B46_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083291.1"
                     /translation="MDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAY
                     GLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVV
                     NLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITT
                     YTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQF
                     PWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQS
                     KHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAAD
                     NNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEIT
                     TKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
BASE COUNT        12504 a         8474 c         6960 g        13272 t
ORIGIN      
        1 tttttaccct cttttctttt tgtatttaga taactccctt atcttgcaga actggggtga
       61 ttttttcttc aatttcattt tttgaaacaa tatttttcaa atagatcatc gccgttttag
      121 gatcaactgc gaacttttcg aactgcgaac gaaaattttc tgctgtgatg atcacatctg
      181 gtttcatgga aactgcggaa gaaatatcac aatggtccag tttggcttct actcctaatt
      241 tgttcaaaac atcctcagca gacatctgtg ctgcaaaact acttcctaaa cctgcaccgc
      301 atacgaataa aatgttcaat ttgttcatct ttgttctccc ccgtatcatt tattatttct
      361 caactactga aaaaagcagt tgtttgaaat cttcgatatt tgtgcattca atcaatcgat
      421 ccaacttctc ttcgttgttg atcaattcaa ttagttcttt catgatgttc agatgcgagt
      481 aggagtctac tgctgccaaa caaaaaataa tctttactgg atccatttca ggattaccga
      541 aatcgattgg ttgctcaatc gtagctacgc tgactcctaa tttattgacc ccatcttctg
      601 gtcgggcatg ggctagtgcc atgtgtttgc caatcacgat atacggtccg taatcattca
      661 ctgcacgaat catcgcttcg acatagcgct cttcgacaat gttttcttta accaatggtc
      721 tagcagctag ttcaatcgat tcctcccaag tagtggcttt ttctttgatc atgatattgc
      781 tgtccgtcaa aatatctttt aacataggct ggatctccct tttgttgatt tctaaattgt
      841 ttgtttcgaa taatttttct aacttgccat agatgattcg atctactcgg ccaccacttt
      901 ctttcatgac atctattatt tgagtgaata actccgtcga ttcatgactg ttaggctcta
      961 accgctgtag ttcttcattt cttttcaaaa agtcttctat aaccaagata ttcgtctcag
     1021 ttaaaatcgg atcgacaact aaaagcggct tatttcgata attcaattga aaggtgctga
     1081 aaaccagatt gacatccaat ttatctacta attcgatttc ttttgaactt aaaactgcag
     1141 taatctcgat ctgaggaaac ttttctttca gattctctgc taacagattt ccagtagcca
     1201 tcccatggtt acagacaacg actgatttat aaacataatt caagtctcgc ttgattacac
     1261 tggcgatcgt cgaaaagtgg atgacaagaa aagctatctc gtcctccagt atcttgccac
     1321 caattacttc ttcgatcgta ggaacaaaat gagtgactgc gcggtagatt gggaaataat
     1381 ttgttcggat gttttctttc aatggattca tgacttggat atgattcttg attcttacaa
     1441 tcagtgcagc catgtgctta tagagatttt cgaataaagc ttcttccttt agatggaaag
     1501 gtacgtgtgt ctcgttctct acaaattgga tcaattggat cgttagtaat tgagcttgta
     1561 cccactcgat tgaattggag atatctctag cattgaacgt ctcaatagtg aaacgaagat
     1621 aatttctttc tacttgcggg acattgtcca attgaaattc ctcgatcatg tggtcaataa
     1681 atgcagaaaa gctactgtct tttatttctg tatttttcca attaatggca gagataaatt
     1741 catgtttcct gattcgctgg atccagatca atgtaaaaag caggatttgt tttttataaa
     1801 tatcatcttc ttgtttcaac aacttattgg aatagatctc ctgtattttt ttgatttcca
     1861 ccaaaggaaa ataatggaga aagatttttt gagccggtgt taattgtgca tcttgattca
     1921 taaagaagtc ggtcattccc agattctgat tgataaaatc atagatcatc gtacggatag
     1981 atcgttctga acctttataa accagtcctt gctttccata actgacgatc tctatcccat
     2041 aactgaccag tgttgctcgt aaccgtctca tatcttcatc taatgtactt ttagagattt
     2101 gaaattcttc ttcttttttg tttagatgga tcgggattcg gctgtaagca acggctaaaa
     2161 ctagatcaaa aatacgttcg cgccgattca aaaagccgtc tttcacatct tttatttcag
     2221 tttctaatcg agcttgcttt tccttttcca gcttcaacaa aaaacctttg ttgcgaattg
     2281 tacggatctc cggaagcttt ttactgctca accagatatt cgcttcaaga acttcctgac
     2341 ggatcgtccg ctgactgaca ttgaactcac ttgctagatg ttttgctttt actggatatt
     2401 gggctgtcat gattctgtga agtatttcaa ttgttcttgt tttcatagaa atgatctcct
     2461 tctgatttca agtatatggt tccgcttaca taataaaaag tagatatttc tgccacttga
     2521 agtggaaaga aagtaagcaa agcaaacaaa aaataagcca tatgcttttt catctcctca
     2581 acgaaaaaga catacgccta ttattacaaa aattttgccc atctatacaa aaaagaggtt
     2641 gtgaaaaagc tgtcctgcat caagtaataa gaacaactaa actaaaatag tgcttcctat
     2701 ttttcgtttg gttgttctta ttatcgcaga tgcaagcttt taaacaccat ttagtggaga
     2761 ggttgtgaca gagatgttca gccttgagcg ataagaagcg attttgaaaa atcgcttccc
     2821 atattttaca aaatcgtgcc ttattgccga taggctatct ctggcacacc gtttattcag
     2881 aaaaaggagc tgcgacaagg cttttgtcac aactccgttc ataaatattt cctgtcaatc
     2941 cagcataatc ggctgaataa aatcggcttg ccatcttttt tctttctcag aaaaatcaaa
     3001 ataccatatc tgagaaaaat actgtcgttg tgtcttactt cctgagatca atcgcccatc
     3061 ttttcgatcg actgaataat cacagcatct gaaatcaatt cggatggcaa aactattttc
     3121 gtggatctgt cgataatttc ctacttcctg aatagttatt tttttagtat gatttcttac
     3181 accgtctgct ttgttttttt gcagaacttt ctgatgttcg gcaaacagct tgtctgtata
     3241 acagcttctg gctttttcag gagtttcttc ttcccaagcg gattggatct tcatgaatat
     3301 cttttggatt tccagaaact gtgctttctt ttctttagcc gttcctgaca tgcgggacaa
     3361 tatctccgct ttcagccttt tcttttgtct tttattcttg acgatagaaa aagcagaaac
     3421 ccctcctact gacaaaacag ctaatgcaag aaatcccgtt tgctcataac tagaaggacc
     3481 tgtacctgga ccataatacc cataagaatc tctgccgtaa gaacgactgc caaaataact
     3541 gcccccatat gaacgaccac caaatgaacc tctgcccgaa cttcgaccgc tagatgtacc
     3601 tccaccatga ccacccgcga cagcgaatcc ttgggtcggc agagcaaaca aacataggcc
     3661 cactaacaaa atgattgcta aataattgat ttttttcatt gtcttcctcg ttatctttta
     3721 ttacaaaaaa ctatttttat gatagccttg ctaaaaggaa gagtcaataa ccagatgcat
     3781 aaactgtttt tttagcaatt ttgtcttatt tagtcattga gtaacatcgg acaaatgtat
     3841 gtgtcggcag tccataattc ctctatcttc agcatgaaca agcttgcaga caagagatct
     3901 gcgcttcctc caggagagat ttggttttcc tgacaaaagt gcaacagtgc tgtaaatgga
     3961 cgatttggta aagcactttg actagcagca tgagcaagtt tttgcatttc cactaacgtt
     4021 ttctgatcac tgcgatgcaa tacgcatgtg tcatctagcg aactcatcaa aactaacaac
     4081 atatgcattt gtttctgtac atatgaatca ttatctagct ccttagctgc ttgaattgca
     4141 agcctgatat gcggataccc taatatcgct tcttcatatg caccatttac gccatatttt
     4201 tgcttcaccc tatgaccatg agtggttttt ttatgacgat attttgtatc aggaaaagca
     4261 gcaagcttgc tgacatcatc aagtattttc gataaagaaa aaggctgttc catactgatt
     4321 ttttgtgcaa tgacactgat caatagcccc attgcccaaa tcgctccttt atgggtattg
     4381 atgccatttg tagcaatcag cattgccttt tccgcttttc gcccaatctc agcaattctt
     4441 tctcttaact cctgatcaat agcatgatgc catgctattt gagccatttc ttcaaaaaaa
     4501 ggagttagcg tcttagcaga cttaagaaat aaagcatagt ccatatcgga atgtgcaccc
     4561 gcatccagtg catccactaa tccaggtttt ggacttaatg ttacttctgc gatcaatgct
     4621 tcctctacca acgcagcgag ttgctgagtc actgcttttt ttcgttcgtc atttatcatg
     4681 acactcactc cttcttccta ctattacttg ctgaaacctt ttttgatagc tacccaaatg
     4741 atatgaagta accagcttaa agcaaatgca caacttgccg tcattagata agctacaaga
     4801 atcatgactc ctgaaggtaa ctgcagccaa ctatctttaa acaataataa cagtacttgg
     4861 aaaacaaata cattggctaa atacgtacga taagcataaa cagacaacca tttgaacaat
     4921 ggcaattttg gcgaatcgtt tacaatcatt ttggaagcgc ctatgaagac agcaaagatg
     4981 atgactaaac tgtataaact ttggatcgta ttcaagtaag gtgcattcgc aaagctcaag
     5041 tctcctggat gtgcgagcaa tgaacggaca gaaaagaacg cgataactaa ggcaacaggt
     5101 aagaaaagat aacgtactct gtccaaatat tgatagactt tttcgtgata aacaaatgcg
     5161 gctaccccat aaatcatgaa actagcaaca aaccgatcta ataagtacca gctgtcatga
     5221 taaggcccag tcaatacttg tgtttgatag aacagatacc atgcgacata taaaatcgtt
     5281 gaaccaatca atactggcca tacatatttt ttattagcaa atactttgta accgagccaa
     5341 acaaaaaacg gcatgaacaa ttggatctgc agcatcataa cggtatacca taaatgaggc
     5401 gctccatcac ctgtgacata tttcagtaaa aagcttgcag tatccgtata cggagtttgt
     5461 tgttggactg ctggaaatag ccaaagataa gccgtcgtcc atagaagata cggaacgact
     5521 agttcaaaaa acttttcttt caaaaattcc caataagaca aatgctgact tgtttttacc
     5581 atattataaa caattgcaaa aataaacatt ggtgctgtgt atttaacgat gacgtaaaag
     5641 gtaccaagaa aagctgtatt tgttgcgctg tggctgtttg ccaaagcaaa gctcaatatc
     5701 gtttgcatca taacagccgt tacagctgat actttctgga agtcacttac gttgattttg
     5761 atttttttgt tttcaaacat ctctttctct ctctttttct ttattaccaa agttctttgg
     5821 ttaatttagg tccttcgact gttttcaata gaattttttt ctctggctct ctcaaatact
     5881 cttttagtga aaaaccgcct tgccgagtca ctacttgggt atcgaattgc tcaaaaaaag
     5941 caggatgcga ttgaattacc tgaattggca attcaatcgg tgaatttgcg tacagcagca
     6001 aatcaaaatc actggtttct tttactgccg gaatcccagt cgataattca aatcctaagc
     6061 tgccgccgat tccccattta catcctgata atatgtttct agcagcatga aattgctgat
     6121 aaacaggata atccatgatt ttctcttgtc tatacgattc ttgttcgatg atgtcccacg
     6181 gatgaatgac ttgtttgacg gcatgttttg agatttctgt tgaccatctc tgattgcggg
     6241 caggtccacg taaaccaatc ggtagtattt ctgcgtgatc cgtcgacgca cgacgaactg
     6301 ttccgaacat cttttcagtt agaacgaccc attcaggcaa ggtgaaccct tctatttcat
     6361 gtttatcaaa aaggacaata tcatgtgctt gaatcatttt ttatgcatcc cattcttcgt
     6421 ccattttggc acggacttta tttgtttcga cacgtccgaa tgttttggct gtttcgtttg
     6481 tgtatcgatt tgtcagcatc gtgtcatttg tattgcggac atcttgaatg gcttcattga
     6541 ttgcttcgat gactttgctg acattttgtt ctgaagcagc ttggtcggtg atatcttcta
     6601 ggaaatgata gatcgctcct agttttgtat aattattgat atcgtaagcg atagctggaa
     6661 ctttttcagc agctgcttcg atttcagcaa ccgaacgttt tgtcacacgt gcagcacttt
     6721 ctttagacat cgcttggacg gtaatttctt tattgtttaa agcaatcaag cggcttgatt
     6781 gtaatccatg tgctaaaaat gcacctgaaa tagcatttcc tgcaatcaat ccaataactg
     6841 gatgtccagc ttgacggagt tttgcataag cagcagcact attagccaaa gccacatgga
     6901 taccgatcaa ttcttcgtta tagccgtatg cttggcttgg tacatcgatc acaacgatga
     6961 ttggccgttt cacttttttg tctttatctt cttcgtaaac gtgatccaaa atttgtccca
     7021 tgcggaaacc ttcaactaaa ccaacttcac cgtttcgtac acggtacatc gggtttttag
     7081 gatcaggtac gattgatgtc acgatggctt cttctatgaa tttacttgat ttaccataat
     7141 acacagtact aatagaagaa gttggatttt tcatgccagt tagtgccgca aaccaatcgt
     7201 aaccaacact tgctgcagct ggttcatttc cttctgctac ttctggtaag tcaattgatt
     7261 ttgcttgatg gttagcaatt gcttcattgt aatcgtcaat ggccattggt tttgaaagat
     7321 ctaaagaatc aagtaatgat aaataaaatg ctgagctttc actgcgatgc gtgtcttttc
     7381 gttcgtccat tgctgcgatg acagcagctt tgattgcttc tacactatca gtcactaatt
     7441 catcaatgat ttttgattct acccgttgac gagatcctaa tgtattccaa atcaagtctt
     7501 tgtcactaga atcaaattct cgaacgcctg cttcttgttc aataacttct ggtccattca
     7561 aaccgactct cgcttttttt gttgcgatta aataactcat taatgctgaa gtaatcgaca
     7621 taccgccaaa tgatcctaca cgtcctggaa tcaaaccgat gactggtaca tattgtttta
     7681 gagcaatgat tacatttccg atttctgaaa tagataacag tccgtagttt gcttcttgca
     7741 aacgaacacc acctgtatct agtacaatga ctggaaagat ctcatttcct tttttatttt
     7801 cttctaacac atgttcaagt gcagcaacga tttttgcacc ggaaacttca ccgataccgc
     7861 caccttggaa ttttccttcc atagcgatta ctactgcttg tttgccattg atcgtgccac
     7921 ggctgacaac gatcccgtca tcactttcag ggacgattcc ttgtgctggt aaacttggcg
     7981 cgatgatttg atcgaatgga tcaatcaatt cacgtccatt gtcaagtaga gcgttcactc
     8041 gttctctggc gtttaattct acaaaactat ttttcatctg aaagttcctc cattgcttgc
     8101 gtcaatcgta ggtacacaac tcctggtgta gcaccgaagt cattgatcac gatatctgct
     8161 gaaattggat aacgatcaaa aaatctcgtt aatacattgt tccatacttc gccgaagcca
     8221 tcgcttcctg tacaaatttt tacagtagat tctgtttttt ctgttggaaa catcaagatt
     8281 tccaaatctc ctgaaccgac cactcctaca tggattttct tattgatcgg ctgtttcgtt
     8341 ggatatttat aagctaattg ttccatggat ctctcccctt tttgtaaaac agagcataca
     8401 cgttctgctt attcgtcttt cttcttccat ctgggtaaat ggcgacaaaa ggtttttctc
     8461 gccaatttta tgaccaagtt ctgaatttca ttggtggttc atacaggcca tctgaccatt
     8521 cgatcaaatc atcgatggtt tgtgcagcta ataatgaacg tttcgcttca ctacgtttga
     8581 tgcctagatc ttctggtaat ttcacgatgc cggctgcacg caagctatct agtgattctt
     8641 tcgtgctagt cattccaaga ggtgtaacac cgccaattgc tgcaattgct gcttgtcgtt
     8701 cttcttttgt ttttgctttg tacaaataag caattccttc ttctgtcacg atatgtgtag
     8761 tatcttcgct ataaatcatc actggtgcat tggctaagcc tgcttgtttt tgaacagcta
     8821 gtgcatctaa ttcttcgaca aagactggtt ttttatttga accatatgtt tcaaccattt
     8881 gaacaactaa tttttggcct ttccctaatg gatcatcatt gtcgcgaagt gattgccatg
     8941 ctggtgtaga atgacgacga ccgccaggat tatgtcccat gttaggcgca ccgccaaaac
     9001 cagataaacg tccttttgtt acggtagaag agtttccttc gtaatctaat tgcaaggttg
     9061 aaccgataaa catatcgatg gcatattgtc ctgcagcttg tgataatgtt cggttactgc
     9121 gcatcgtgcc gtctttaccg acaaaaaaga catctgggcg agcagcaata tatttttcca
     9181 taccaacttc gccaccaaaa ctatggatgc tttcaaccca acctgattcg atggctggaa
     9241 tcatcgttgg atgtgggttc aatacccagt tcttcgcgat tttccctttt aaccctagtg
     9301 attcaccata agttggcagt aacaattcga tagctgctgt attgaatcca ataccgtgat
     9361 tcaaagattg tacgccgtgt tctgcgtaga ttcctttgat tgccatcatt gccattagaa
     9421 tttgcaattc tgtgatgttt tgaggatctc gtgtgaataa agcttctaat tgataaggtt
     9481 tatctgcttt cacgacaacg tctacccagt caccagggat atctacacgt ggtaaagaat
     9541 cgacaacttc gttaacttga acgatcacga taccatcttt gaaagctgca gcttcgatga
     9601 ttgttggtgt ttcttccgta ttgaaacctg tgtacaagtt gccgtcttta tctgctttat
     9661 ctgcagctac taaaacaaca tttggaatca aatcgatgaa taagcgtcca taaagttcca
     9721 aataagtatg gatatcacct aatttcattg tgccgtcttc aatcatttgg gcgacacgca
     9781 agctttgtgc accagcataa gagaaatcga tcttgcttgc gattcctttt tcaaaaatat
     9841 tcaaatgttc tggtcgtgag atactagaca tgatcaaatg cagatcattc actttttctg
     9901 catctacttt ttcaagactt tctgaaagaa aacttgcttg tttttgattg tcgccttcta
     9961 atacaacttt gtcgccggga gcaatcagtt tttccattac ttctacgatt ttttcagttt
    10021 ctacatactt gccattgagc aagttgctga ttttttccaa tcgctgcgct tttttttcgc
    10081 gtttagaagc ccagttgcgg tttggttttt ccataattag tcatctccat ttttgatata
    10141 gaaacgtggc gtcatcaata ccatgttggt ctagattgat cgtacggatc cctttatcta
    10201 caccaaattt tgtatgaatc agctttgtca aagtattgcc ggggggaaat tcaatcgtca
    10261 gatccattcc taattctttt gccaagtcca tcatttggtt ccactgcaca gggaaagcta
    10321 ggttttccaa caaatcttta cgaatctctt ctgcgtcctg agtggctctt ccatccgtat
    10381 tttttatata caaacatcta gcatcagata gatggacaga ctgcatatac ggcttgaatt
    10441 tcttgaccgt atcattcatc aagagacagt gagatggcac agggactttc agcagcttcg
    10501 ctgtttgcgc ttggtgccgt ttggcttctg ccaacgtaac ggataaacct tcgatactcc
    10561 cagataacgt atgttgcatc ggacaatttt catttgacag ataaactgga tggtcaggat
    10621 caaatgtttt tgctacaatt tcctcagctt cgcttctcgt cacacctact actacgccca
    10681 ttgcataacc gcttggataa gcttctttca ttaagactgc gcggtgatag actagttccg
    10741 ctgcattctc aaatctcaaa gatcgacaag caacagctgc acaaaaagca ccaatcgaat
    10801 gtcctgctgc aatatcaggc tgaataccgt ttttttccat ttcatctaaa taaaatactg
    10861 cttttgtcag taatgctagc tggataaaaa ccgtatcttg atagttttct tctttatcaa
    10921 ctaaccgata ccccgtcaca ttttctaaac gttccatgta catttcaggg atatccgcta
    10981 acatccctaa cttctgactg ccttgtccag gaaataagaa acctgtactc atgagcctcg
    11041 cctcctcagc acttactttt tccactgcta tcttagtcaa tttctgctat aataaaaaat
    11101 gccaaaaaaa gaaaaaacca taacaaaaag gtatggatgg gatggataat tatgaaattg
    11161 cttcatctgc agtattttaa aaaagtattc gaaacaaaaa atgttaccca agcagccaaa
    11221 gaattattta tttctcaacc tgccttgagc cgtgcgatca aacatttgga aaacgaatta
    11281 ggtgtccctt tgttttacca tagcggcaga aacattgaga cgaccgttta tgcagaagaa
    11341 ttttatccat atgcagtcaa gacattagac acattagaag aaggaatcaa attattgtct
    11401 tcggtcaatg agaagatcgt tacttccgtc atattgtatt tagaggtagc ctctgtttct
    11461 attccgaact tggtccgaat ttttatggaa aagcatcctg atatccaatt aacgatcatg
    11521 caacatcatt taccagatga tattaccgat cctgtactgt acatcacttc ggagatgaag
    11581 cctggcatga ccaatattcc tatcatgaaa gaacctgttt atgtcgccat tcctaagaaa
    11641 catcctcttg ctaaaaaaga acatctgcaa ttagaagata tccaacgcac ccccatcttg
    11701 atgctgtcaa agagcaacgc cttccgtcat acactggatg cagcacttga agcgaaaagt
    11761 atcgaattga cgatcagttc gacgacagat gatcctgcga cattacgttc tattgtcaag
    11821 caaggattag ctgttagctt tttcccaaaa gtttcttggt cttacgataa aaatgatcct
    11881 ttcgtcttgc gggaaatcaa agatttgccc ttgacacgaa caatttattt atcttctagc
    11941 ttcacagaag atcatccttt gatcaaaatg attgcaaaaa cgatgcggga attttattta
    12001 ggaaaataaa aaaaaaagag ctgggacaaa attcttgtcg cagctctttt ttcgaacaaa
    12061 cggtgttcca gaagtaattt cttagaaaat aagccgaaat ttcccaaaaa tttgagaagc
    12121 aattttcgta aattccttct tatttcttga agctaaacat ttctgtcaca acctcttctc
    12181 tattgtccta gacattcatt gcgttgattt aaaacttcca tcaatcaaat ctgctagttc
    12241 atctagcttt tctattttac gtagatcaat agagatccaa tgttctttat tcatgtgata
    12301 ggctggaaaa tacccctctt ttcctcgcaa aattccgcca agttcaggat cgaccttcaa
    12361 atcaagaata ttgatgatcc tatggtcact tgcatcatca tagatttttt cttctggaac
    12421 agacatcagg accccaaacc attttttgct tttttgatgt ctgaatacgc aataatcagg
    12481 aaatttttta aaaatatatt ctggagacaa attgtatgtt tcattgatgt gatcgattag
    12541 ttcttttttc gttagcatct gcttccatct ctttcttatt gattcgcttt attcaaagat
    12601 accacgtaag aatagaaaaa tgaatacaag tagttgctaa tcaaatactt cgatattttt
    12661 cttttttatc aactcttaaa tgttcaatcc gcctgtgttg gtcttgctac catctactat
    12721 taaaatgttt attattgttt gcccacctat tttttgattt tagtggtatc atttttcaag
    12781 ttcatgatga aattcttatc atcagtaaat gctccagaaa aataaacaat gattgaaaaa
    12841 tcagttgaaa cttgaccacc gtttattttc tccgtattct tagaatagaa gggagtctga
    12901 ttgatgtatt cactaatgaa aaaaatcatt accgaaaaag atattcgacg tcatgtttct
    12961 ttagtagaac aattgctgaa tcatacaaag ataaccgtaa atgaattagc tgaaatcata
    13021 gggacaaccg aacgaacgat tttttctgac cttcaatcta ttcgctctca gctgcccgaa
    13081 ggctgggaca tcatcagcga tcaagcagga atcagtttgc aaaaccagca aaatttatta
    13141 acaaatgacc tttgggagat ttttttcaaa caatcagtca gtgtcgaact gctgaaaaat
    13201 ctgcttttta caaaaaaagt tgctgtccct gattttctag ccgaccatgg gctttcttat
    13261 gggacattga aaagacatgt gactaaaatc aatcagcgtt tagcaagtta cgacttgcag
    13321 atcgatttga cgaaatatac agcttgtatt cttggaaaag agcgtgcgat tcgtactttt
    13381 tatcatcgat tgctgattcc tttcactcat aacaactatt ttttcgagga ttattcgatc
    13441 catgaatccc attattttca atttttaaga aacctcagtc aaactgaact agcggttgaa
    13501 acagaagaga ttttcggaac atgttggttc tttatcaata cgatccgtat aaaagcgaat
    13561 tgccgattag attcttctat ccataccaac agcacactct cctccctgta tgactctgca
    13621 ttgaaaaaat tgtatttaac ggaaggtatt tatcttaaag acactgagct ttcttttgct
    13681 tctttctgct ttctggaaag ttggaattac aacaataact atggacaaga aaccgctcgc
    13741 tgcctgcatc attctccatt tttagaggtc ctcgaaacgt ttgtagagga actagcttca
    13801 gagctgtctc ttgatcaact aaaaaaaaca tctctgactg ataacttggc tctcctcata
    13861 ttaaaatatc atgaatcgcc gatattgatt gaagcattgg accgtcaata ccatcgtttt
    13921 ttagaaacat atgatcagca atatgcacac ttgtatcaac agaaagagaa tttactgaac
    13981 gatctaaaaa aagaaataga aatcaatgaa ccaaattact ttctccaact cttgtctcta
    14041 ctgatccaac aaacgatttt ttctatcaaa ccgaatttgt tcaatgtcta ttttattttc
    14101 caaggagaac cttcatggaa ggcgttcttg cagcaggaat taaaagatta cttaggaaaa
    14161 cgtgtctgct tcttacctat cgaactaacc gatctttcca ctatttcttt tgaaaaaaca
    14221 gatatcttga tttccaattt ccctctagat tctctagata tccctattgt atacatttct
    14281 tctattccaa cgaaaaatga gctcaatcgt ttgactgaac tcactttcaa aactttttta
    14341 tagtaacggc tagcgactgt attctctagc tgttttttct tttttgcgaa acagtttgtt
    14401 catgatccct ttttctattt ctacaactaa gaatagaaga atcacatttg ctccaatcaa
    14461 taactgttgt gtcatcgtta aacttactgt accgatcagc tgctggccga ctggtagaaa
    14521 aataaccatc ccttgcaata aagcaaggat acctaatgaa agaaaaagcg ctttgttttg
    14581 caaaagacct tggttcaatg acggcttgga caactctctg caattgatca tgtaaacagc
    14641 ttgagctaag acgatatttt gcagaagaat ggtttgctgt aagctttgcc cttcaaactg
    14701 catagccaat aaataggcag gaaccatgat cagtagggag acatagatga tccgaaacaa
    14761 atgataacca gataggattc cttgctttgg atctcttggt gggcgcttca tgatttctgg
    14821 atctggcgac tcgaacccta gtgcataaga taaagtgatt gtcgttacca tattgaccca
    14881 taaaatctgt actggggaca aaggtagcgg gcgattcagc ataagtgccc aaatcaagat
    14941 caatccttga gctaatgcag ttggcaagaa gaaattgatg gttttttgca aattatccaa
    15001 aatccgccgg ccctctttta cggcttttgc aatcgtatgg aaattatcat ctcctaaaac
    15061 catatcagct gcctgtttgc ttacttcact acctttgatt cccatagcaa ttcctacatc
    15121 ggatcttttc aaagcaggtg catcatttac gccgtctccg gtcattccga cgatttctcc
    15181 atttttctgc aaagctttaa caattcgcag tttatgttct ggtgtcgttc gagcaaagac
    15241 atcgactatt ggcacttgct gaatcaactc ttcatcagac ataagatcca aatcgatccc
    15301 ttctaaaact ttggcagtat gttttaagcc aatttgttcc gcaatcgctt ttgctgtgtc
    15361 tttgtgatcc cctgtaatca ttttcactga gatccctgct tgttggcatt cctttacagc
    15421 tttgactgca ctttcttttg gcggatcgat caatccggcg atccctgcaa aagttaattg
    15481 ggttaaagtc tcatgcgtga gttcctgttt cgaatctact tttttataag caaatcctaa
    15541 aacacgctga ccttttctcg caaattctgc agctgtctga ctccatgctt cttgttctgc
    15601 agaagaaaga gttgatttag caaataatac ttctggtgca cctttgacgt aaatcaccgc
    15661 tcctttttcc tccgcatgca tcgttgccat atatttgtat tcagaactga acggaatctt
    15721 tgcttctatt tttttcaaag gtagattggc tgtttctgtg tgtcgaatca gcgcaacttc
    15781 tgtcggattg ccacttaaat tttctgtttt ctgttcttct tttgttttta attcctgaca
    15841 gtttttcata atggacaaca cgcaggcttc ttcttttgca gctacatcca caactgtcat
    15901 ttcatttttt gtcaatgttc ctgtcttatc tgaacagatc actgtcatag aacctaatgt
    15961 ttctaccgaa ggaagtcctt tgatgatcgc cttttctttt gccatttcat gtactccaac
    16021 ggatagaatc atggtcaaaa cagctggcaa tccttcgggt accattgaca ccaccaaagc
    16081 aatcgtagcc gaaagaagga tatgccaatc cataccatat cgaaatgtcg taaaaataac
    16141 taagaatagg ctcaaacaaa gaatgccttg aaaaatttgt ttgtttaatc ggttgatttt
    16201 tttaatcaga ggcgtcgttt gttgttcgac cgattgcaat gcttgattga ttttaccgat
    16261 ttctgtcgtg tttcctgttt ctacaacgac accgactgca gaaccagatt gtacaagcgt
    16321 tcctgaaaaa cccatatttt tctggtcgcc gatcgattct tcttctgtca aaacggcatg
    16381 acttttttca gatgcttctg attctcccgt caaaatcgat tcttccacta ataaatcatg
    16441 tgcttcgatt aatcggatat ctgctggaac gacatctccg gcacgtaaga caatgacatc
    16501 tccttttacc agttctttcg cagggatttt ttcttgcatc ccgtttgata gtactatcgc
    16561 ttcttgtccc atcagctgct tcaaaccatt caatgattct tccgcttttc tttcttgcca
    16621 gtaactaacg agtccattga cgatcactac gaataaaata atacttcctt caatatattc
    16681 actagaagca aatttcaaaa aagaggcaat gatcaacacg atcatcagca gatcagtaaa
    16741 atgctttgct atttttttcc atcgttttgt ctgctgcctt gcttttattt cattatatcc
    16801 ttgatcagtc aggcgtttct gtcgttcttc ttgcgacaag ccttctgttg atgtttgata
    16861 gtttttcaat acatcttcta ccgtcaatct gtaccagttc atcctatccc ctcctgttta
    16921 attgatactg ttattatacg cagggaagtt ttttcaggcg tatcgattat ttcagggggg
    16981 tctgaaataa ttcgttttgc aaaatgtatc tgggcctaac tgatatttct attgcttatt
    17041 cttttatata caaaatttaa gaatacaaac cagataacca ataaaaaagg aataaagcgt
    17101 gtcgtgcttt cgaataacaa gctgaaagca attgaagtaa aaaacaccaa caaagcaaga
    17161 tctttatatc tttgtgtagt gttccgtttc ttttctttaa taaattgaac atgtgcacac
    17221 aagatcgaag accacacaaa tagaaaacaa ctcgttgtca tgcctgccaa aagctgaaaa
    17281 aacaatcgac tatcagctac aaaaaaatgt agacaaagac cggcaaaaaa taacattcct
    17341 gtaattaaaa taccgtgtaa aggtacgcct ttcttagaaa gcgcacaaaa ccaatggggc
    17401 aaactttttt cttttgctgt ttgatataaa atcctgctag tactgtagac tgcactattt
    17461 gctgaggaca aagaagcaac caaaatcatt agattgacaa ttgctgctcc gccttggcaa
    17521 tggatcactt gcagcatagt cactaaagga ctttctttta aatctaaaca atgccatgga
    17581 acaacaaata aaatcgacaa aatcgttcct atatagaaaa agagaatacg ccaaggcaga
    17641 cagttgattg cttttttcaa attcttttgc ggatcacttg tctcgccaac ggttaagccg
    17701 atcatttcaa ttccagcaaa actgaataaa accataggaa atgagactac cattccagca
    17761 aagccaaacg gtagtaattc atcgatctgt ccaaaatttt ctttgattcc gatagactgg
    17821 gtatgaaaat ctgagacact caatcctata cccaataaga taaaaaggaa aatccccaag
    17881 atcttgatga ttgagaaaaa agattccaat tcaccaaacc acttgactgc aacaaggttg
    17941 actgccatta agatcattag tgcgctacct ccgcttatca aagctggtac atgtacaaac
    18001 cagaaatgga tatacaatcc tattgctgtt aattccgcaa gtccagctcc aagccagcaa
    18061 agccaataag tccaattgac aaaaaatgcc cactttttgc ctaaataata gtcaacgaaa
    18121 tcactgatag agttaaatgt actatttgct aaaaataaat cacccatggc ccgcatcaaa
    18181 ccataaatag ccaaaccgca tatcaaataa acgagaataa cagctggccc tgctgatgca
    18241 attgcctttg ctgaacctaa aaataatcct gttccaattg cgccacctaa tgcgattaac
    18301 tggatatttc gattagttag agtccgtttc attgccggtt tttccatcaa gttcaccttt
    18361 ttctttttca agcagcatgg cgacttcctg ttcccaagaa ggtttcttta ttttttcaat
    18421 acagagtggt actacaaaca aaatgacact gatggctgtg actgcaatta gattgatcca
    18481 tgttccagct ttcatcgtca gtccgacaga aaatccgatt gccgcaaata aaacaataac
    18541 gacagtccaa actccaagat tgctcttttt ctcttttttt ccaattctaa atggtcgatt
    18601 caactttgca tgtgtataac gcaatctcaa aactgcgatc cccatgatga catagacgat
    18661 acagtagata acagtagttg aatttacgag catcaaaaat gcttgattga cgccaggaat
    18721 cagcaaataa aaaagtgcaa agattgagat aatgacagac tgagtcaaaa taacggattt
    18781 ggatacgtca aattgattcg tcttccaaaa attgaatttt ggtggatacg caccttttct
    18841 agcactttct gtcactgtct ttgctggtcc tgaggcccaa gcagataatt ggactgctac
    18901 accaatgaag acaagtaaac taaacagatt cacaataaga tgtggcaaac caagaatttg
    18961 gcaataaatc gaaatagatt gcgcgatatt attcaattcc atttgtcctt ttggtacgac
    19021 atttgctaca actaaagcat tcaaaatatt gacgaaaaat ataaaaatta acgcggcaaa
    19081 tactccgcga atatacgttt taactggatt ttccaatcga gtgatataca cagaggacat
    19141 ttcgatacca gtaaaaataa acatgatcgg cgcaaaataa acaaatgagg atgcagtcgt
    19201 agtatctggt attaatttgt cccatgaaaa actgcctaat gttccggttg gtacgatccc
    19261 aactttgatc cacgcagcaa accctaataa gagcatcatc gtcaaaggca cgtacaatcc
    19321 taaccaaaca ccgacttttc cgccgatttt tgacatatca aatttcaaat tcaaaaaagt
    19381 gatgatccaa taaacgacta aaatgacaac taatgtaaat ttgctgttca aaccaagcgg
    19441 cacattatcg ataatattac caaacaacgg agcgagtgct gaagcgacca tcaccatccc
    19501 aggaaacatt tggacccata acagccatga gacaacgaac ccccattttt ttcctagagc
    19561 attcgatacc caaagttctg gaccaccttt ttggggaaac attccagcaa attcaccaga
    19621 gatcaatgag attggaaaag cgtacaataa tactgcaacc agcatgtaaa aaatcatcgt
    19681 ccagctcgtt gctgctacat ctggaatatt tcgtacactt gctacaaggg cgcaggtcat
    19741 tccgataaag gtcatggtcg tgatttgctt tgatgaacca gtatctttca tctttattcc
    19801 ttctttcaat agcgacatat gtttttgttt tcttttattt ttgttcaaga acagaaatga
    19861 caaggtttca tcgattcagt acaaaaccca gtatcaccga aacatccatt gaacgatctt
    19921 agtgaactgg gtttgataca tgaaacgaac gatccaactt atagaacgtt ttataaaaaa
    19981 gaatatcttg gcgaggaata tatgaaaaac agggggaaat acaattgttt tgatgaagca
    20041 aataattgtc tgttctccat atgtctcttg ttattctttc acacaaaaat cagttctatt
    20101 ggatgatttc gtttagtttc ttttccattg ctttggcgat tttttttgta taaaaattga
    20161 aaattcgatc atcattcaaa taaggagtca tgatagccgc acgtagcaga gtgattttct
    20221 gttctttttt ccattcttct ttcgttagac ccattctttc aacaaaacga atcggagaat
    20281 caccgtaatc ttcttgtgta aatgtagtat gagaagtaat aaaccgttca ccataaacat
    20341 cgccatccat ataagaagac cgatcgaaca ttttttcatt taattcattg atagcgttca
    20401 aatctgtaca gccttgttct ttgaataccc aattgaccat attaaaatca ggatgattca
    20461 atggatagac ttcgattgtt tttcctttaa cagtaaaagt cagatgatcg agaaattctc
    20521 tgaatctttg agctgcctcg attgaagccc caatcaattg accatatccg gtcacattca
    20581 atggcaatac acgatgtgct gtccagactg cggcggcggt tgctccagct tttgatcctt
    20641 ccaaaatata agctcctaac atatctggag cctttactga tttttcaaac acatatggcg
    20701 cgaaataaga aatgatattt ctcatatttt tatgtttgat tacgattcct ccagcagcat
    20761 aagggacata gcccatctta tgaggatcta tcgtgacaga atccgcttct gaaatagctc
    20821 tgaacccttc atatacctct ttgtcaatcg ttacgcagtg gtggaaaaca tgatgttctt
    20881 caaagaattc agctaaggaa gcatacggca caaattctcc tgcttcgttt agaaacagtg
    20941 aacgcgcata gccgccatat gcggcatcaa catgtaaata gaaatagatc ccttcatctt
    21001 ttaacctttc tcttaattgg acgattttat ctacactgtc tacttgacct tcttctgtcg
    21061 ttcctacgac agcaaccaca cctagaattg ggattttttg tccagctaat tcacgaattg
    21121 ttttttccaa agcattaata tccattcgat aatcctcttg aactgggata gcaaccatct
    21181 gatctaagcc gactccgcaa atatctaacg ccttcatcca agaatagtgt tttgtttgag
    21241 ggaccagcca tttccctaat ctttggatat tttttccgct acgggaagaa gctgctttga
    21301 cctcatccat ttcttcgtcc gtaaagcttt cggtcatttc caaaatttct tcaacagata
    21361 aattcaaaag ttcccactca gacatttttt tcactttttc aggcagcact tccttgacag
    21421 ctaaaggaat cgatttgatg caacgggcgt accataatcc ttcaagatta gcaatagaac
    21481 catctgctgc aatgtgtccc catccgtctg tcatattgaa taatgaagca aaatcctgtc
    21541 ctacttctgc ttccatttgc gaagttgcca tagaagattc caatgcaaca ttatttgggt
    21601 tccacagcat cgcatagttg taagctaata aagcaggcat caatgtttca gcattcattt
    21661 gtccccagta acggcctgct gaatgccatg ggatcgatcc tgcgcgtaat cgctgactga
    21721 cttcatccaa gacctctatc atatggtcgc gggtggctag ataatctgga gtgagcttat
    21781 ctccttcgct aattgcaggc atatctgaag gaatataatt ttctctccat ccaagatgtt
    21841 catctaccat cttgttcaac aacattttat aaactggtcc attttcagct ttgtcgccta
    21901 taaagacggc cttgatatcc atatctttca tttttcttcc ctccatattt tgttggtcaa
    21961 tgtcttgcag atacatgtgt ttatctgtaa cacctcttca ctatagcgcc aaaaatgaga
    22021 attttttttt tataacatca aatccacgta gatttttaca aacaaaaata aataaagtcg
    22081 gatttttctg ataattcaac gcaaaaaagc gaacaatttt tcaataaatc agtcttctaa
    22141 aatagataac ctaaaaaaag cgaacaaaca aataaaatga ctgtccttca ttcactaaac
    22201 atatttttat ttttttatat tgttaaaaat tcgcaattta ttttttaata gaattatctg
    22261 ttttccaaat tttataacat aaaaaacctt ttttaacggc atttatatgc tttttcagaa
    22321 attaactgat gatttcaaaa agaaaacatg tcataatatt tcccatattg tgcaaaaaaa
    22381 tacaaggcgg aggctctact atgattgaag aatatattga gaaaaatatt ttaagacagc
    22441 tgtttttatg tgggcaattc tatgtaaata aagaagtcaa tctagaaaag ctctccaatc
    22501 tcctgcatgt ttgcaaaacc actttactca atgatatcaa taacataaaa aaagaatttg
    22561 aagaacagat cgcttataca catcgggaaa aggaccgtta cactttatat ttttcagaac
    22621 atatacctcg atgcaagatc atgcagcaat tgtctcaaaa ttcgttgttt ttaaaaactt
    22681 gtctgcttta tttggaagaa gatgaacctg attatcttca attaactgaa tgtgaattca
    22741 tctctgtcag caaagcttat tcattaaaaa aacaagtatt agcttatttt aatgactgcg
    22801 ggatcgaaat cgaccgctac tcccctcgat tcactgaaat ggaaagacgt ctgctgcttc
    22861 ttaatgtgtc gtatcgttta ggcggtttta attcttggga gctacctgaa tcgttttttg
    22921 aacgtgccga tcgtttcatt gaatccgtaa cggaaaatag tggcagattt tatgataaag
    22981 aaaataaaga aatcttatcg attggttttg ctatcagttt cctacgccag caagtgtgtg
    23041 ctgtgacaat agattcaaaa tttatcgaag aaatcaaaaa acgtcctatt tacaattatg
    23101 tcgaatcagc atgggaaaac actgatttcc aaacctatta taaaaaagaa gaattcgcct
    23161 ttattttgac actattcaat ctttgtaatt atggtttcca ttcttatcag ttgattgcgg
    23221 aagactttca acagctacac caagtgttta ttgacaatac tcccgaaata aaagaactag
    23281 ttgctacctt tgaatctcac ttcaatcaag aattattcgg aaaccaaccg ttcgaacgtg
    23341 cgctgattca tctgatgcgt tcggcttggg ataactatca attattcatg ccagaaaaat
    23401 tttatctttt aaatgaggaa cagacaaatt tatataaaga agtccagaca attttttcca
    23461 gctggtccag ccagttacct tatgatctac ggttgaatcc taattgcatg cgggcttttg
    23521 tgatcgaatt gagcggaatt ctccgtctga caaaagaaca tctgactatc tatatcgtca
    23581 caaattcgga tgttcattat ctgatttatc gggaagcctt ggaagctgta actactttcg
    23641 attttcaagt tgcaccaaca atttattcaa gtatatctga tataaagaag tatgcgcagc
    23701 agtcctcaaa ccgggtatta tgtgaacgta cattatatac accagatgcg gtccaatatg
    23761 aaaatattat tcctatttcg atcaatacga tcgatcgagc tatcatttca gctgtgcaaa
    23821 acaaataagg ggctgtgaca aaaatcatgt cacagcctct tatttgttga agatgcccat
    23881 ttctgtaaca atctcttgat acacgtcagg ctcacaacct cacttatttt ggacatctac
    23941 gggtccgatc tctttattta aaaaataaga aatgatggtt cggataacta ccaacgccgc
    24001 tagttggaaa atatcctgca aggtaggttt gataatcgat tcgatgatcc cagctgaaac
    24061 aagaatttcc aaacttagaa gtatatagcc tcccaacatc tttttaatag cattgttctc
    24121 gctgactcgg tcgctttgca cttttgtatg gacaaataaa tatttcaaaa aatccttgat
    24181 tgatagacaa acaccccaaa ccaataaaca gatagcaaag atatcaagtc ccagtacaag
    24241 tttttcgaaa tatggaagaa gaaactgcat caactctgtc gtcatcctac ctctcctctt
    24301 ctttgttttt ctttgtcttc tatttcctga ttcaagaaat aagaaatgac tgttcgaatg
    24361 ataataataa gtgcaagctt caaaatatct gtccaagtcg gcttgataac tgactcgatg
    24421 atatcagcaa cgactagtac ttccaagctt aacaaaatat aacttgctag cagttttctt
    24481 gtatctgcat tacgagtatt acgttctgca tagtttttct tgacatgaga ccaatcaaaa
    24541 agtttgaata gaacaagaac gatcccgaca attaagatac cgatagaaca aatatttaaa
    24601 atagcaatga tccattctag aaattcaatt aaatttgagc gaaacatata cgctacctac
    24661 tttcttttaa tttgttccta ttcttttatt gtaaaagaaa gttaccttgt tcgtctattt
    24721 aaaagttgtt ctaatgataa cggttcgctt aaatttttct ttttcactct cccatttttc
    24781 tccctccatc cataaatcgt ggaagtatcc aaaacaacgt agcaagttaa aataaggctt
    24841 agtccgtcat cagcttttca aagaagcact gtctcggtgc tttttttgta cccaaatttc
    24901 taaaaataga accatacacc tcctcaaatc ttttcttctt acctacccta tttttattta
    24961 caggaaaatc agacacttct tcttttttgt caaaatttcc tttttcttct cgtcctatca
    25021 tttggcattc taataaaaac gaactatcct tggatataga aagagaaaac aaggaggcgt
    25081 ttttcatgag taaaaaatta atcaaagtcg gcatcggttt aggtttgctt gcccttggcg
    25141 ctgcatatct aggaaaaaag acaggactat tcgaagatga cagtcattta tatgatgaat
    25201 tcgaatcaat ctagcatcct atttagttga atcactacgg acatacttgg aagacattgg
    25261 aggaatcaca atgagaaaaa gtaaagcaat aatgattggt gccggtattg ccaatatggc
    25321 agcggctgtt tatttgattc aagaaggcaa atggaaaggg gatcagatca ccttttattc
    25381 attggatgat cacggttcaa atgatggtgc accgaccgac actgtagtgg atgaatattg
    25441 gaataaaaac catccattag aaaaccaaaa aggctatatt gctcgaggcg gtcgcatgct
    25501 taattaccgt acctatgtag atttgatgga tctgttaagt cgaatacctt ctgttactga
    25561 gccagggatg actgctgaag aagatacgag agattttgat gcaaaacacc gaacctttga
    25621 caaagcacgt ttgatggaag gtggcaaagg aatcattgat ggcggaaaac tgggattcaa
    25681 taataaagac cgtattcttc tgactaaact gattgctatg cctgattcag aagaagaaaa
    25741 actagacaat atcactatcg aagaatattt caaagatgat ccacactttt ttgaaacaaa
    25801 cttctggttt atgtgggaaa caacatttgc tttcagaaca cgtagttctg ctcaagaatt
    25861 gcgtcgctat atgcatcaaa tgatttatga attcactcaa atcgaacatc tagtgggcgt
    25921 caatcgaaca cgttacaatc aatacgaaag tattatgctt cctttgatcg agtacttgaa
    25981 agaacaagga tgcaaaatca ttttgaaccg ccgtgtcact gactgggaat tcaaagaaac
    26041 accgatgcaa gatgaaatca ctgtgactgg tttgaaaatt accaatacac tgactcaaga
    26101 agaagaatac gttgtggtcg atgaagatac agcagttatt ttcactaatg ggtccattac
    26161 agattcagct actttaggag attacgaaac accagctgta gaaaatatgg attatggtgc
    26221 agcttctagt ctctggaaaa aagccagtga acgtttctac aacttgggaa atcctgacaa
    26281 attctttgct gatcgagatg caagcgaatg ggtcagcttt accctgacga cgaaaaacca
    26341 tttgttgttg aatgaaatca cccgtatcac gactcaagaa ccaggaaatg ccttgaattc
    26401 atttatttca accacaccga tcacgccgtt aggccaaaaa gatgtcaata tgtctatcgt
    26461 cgtgcatcat caaccgcatt ttacgtctca gaaaccaaat gaaacagtta tttggggtta
    26521 tttcctttat ccaagacgac gtggcgaatt tgtggataaa cagtatatta aaatgaatgg
    26581 aaaagaaatg ctagaagaac tgattggaca actttcaaaa gtagatcctg gaccagtgaa
    26641 tattcgtgaa aaagaaacag aaattttcga cagcgtaata aataatatcc ctgtctatat
    26701 gccttatgct tccgcgttgt tcaataatcg tgccaaaagc gatcgaccaa aagtcattcc
    26761 taaacattcg accaatctgg cctttacagg agaatttgtc gagcagcctt atcagatgat
    26821 ctttactgaa caaagtgctg tacgttctgg agaaatcgct gctttccatt ttgcgggtat
    26881 tcctatgtcc cgtcttgtca aaacaccgcg ttacgacaaa gatattccta ctctagctcg
    26941 tgccgcaaaa aaaatgtttg aataatcaag aggttgtgaa aaagctgccc tgcatcaagt
    27001 aataagaaca accaggctaa aacagcttct tatgtttttg atttggttgg acttattacc
    27061 gcaggtgcaa gcttttgaac accgtttacc aagaggttgt gaaaaagctg ccctgcatca
    27121 agtaataaga acacccaagc taaaactgct tcttatgttt ttgacttggt tggacttatt
    27181 accgcagatg caagcttttg aacaccattt accaagaggt tatgaaaaag ctgccctgca
    27241 tcaagtaata agagcagcca agctaaaaca gcttcttatg tttttgactt ggttagactt
    27301 attaccgcag atgcaagctt ttgaacaccg tttaccaaga ggttatgaaa aaggcgttta
    27361 gtttcgagta tgaagaaaaa ttttgtgaaa atggttcctc acgtttttgc ataaatttga
    27421 cttaataccg agaaactgat cccttctgtc ataacctctt tttatttatg tctgtttctt
    27481 tttgaaaaca actatttttt caacgtttac tctcttgcca atccaacatc atctaacgga
    27541 tacaactatt tcaatgatta ccattacact aaaaaatcgg aaacacacct ctccgaacac
    27601 aaatacataa gactatagaa tgtttcattt aagaaaaaaa gcgtttcatt atcaaaaagg
    27661 taagatctcc tttaaaatga aattatctgt agtactaata atgcagacaa ttttatgaat
    27721 ctgaaaggag catctccaat gaaagcagtc acttggcaag gaaaacagaa aatggaaatc
    27781 agaaacgtag atgatccaaa aatattagaa ccaacagatg caatcatccg tatcacagca
    27841 acagccattt gcggctccga tctgcatctt taccatcatg gtgaagcagt tttggaaaaa
    27901 gggtatgttg taggacacga accaatggga atcgttgaag aagtgggatc tcaagttcaa
    27961 acattgaaaa aagggcaacg tgtggttatc ccttttaata ttagttgtgg tcattgtcat
    28021 ttctgcctga atcacatgga aagccaatgt gatgaatcaa atccagatca gttatatggc
    28081 gggcttttcg gatttggcaa gttgaacggt aatcattggg gtggtcaagc ggaatatcta
    28141 agagttcctt atgcagactc tacttcattt gttgtaccag ataatgatct tcctgatgaa
    28201 aaagttttat ttctatcaga tattttgccc acagcttatt ggagcgttga aaacgcagga
    28261 gtaaaaaaag gcgatacagt tgtgattcta ggttctggtc cagtcggtct attcgctcaa
    28321 aaattcgctg ttatggctgg agcagagcgc gtaatcgcag tagatccagt acagcaccgt
    28381 ctagataaag cggctaatta caatggggtt gaaacttatt tcttagaaga cacggcacaa
    28441 gctggaacag aattgtatga actggctaaa ggtggcgcag atgtcattat cgattgtgta
    28501 ggaatggacg gattagagcc tgtcaaagaa aaagcgaaaa atttagtaag cttacaaagc
    28561 ggcacaatct ctccattaca gatggcatcg caagctgtca aaaaatttgg aacagtccaa
    28621 gtaactggtg tttatatgac tccagcttct tcttacccgc tgcaagaatt ttttatgcgg
    28681 gatatcgctg tcaagcatgg acaggcacca attatccatc taatgccaaa aatttacgca
    28741 atgattgcag aaggactatt cgatcctaca caaatcatta ctcattcgct gccactatca
    28801 gaagcagcac aagcatatga gatctttgac aaaaaagaag ataaaaatat aaaagttgtt
    28861 ttgaaacctt gaattcagta aaaaatacag gtgattttct ttatgcccaa aatcaaacga
    28921 aatacccatg agatcgaaat aggaaaagaa gaagatatcg aaatacgagg acgtcgcttc
    28981 cgcttctatt tccaaaatcg ttacacattg atctcactgg ctgtcgattt attaaccggg
    29041 attttctata tttttggaag ccttgcttcc ttaactaaaa tccccgatcg atatggcacc
    29101 tatttttatt tggctggtgc tattttcttg acgattcgtc ctattctcag aatcgtaagg
    29161 aacattttta tttacgacga aaaaaaagca atgaagagaa caagcaaaaa aaaagaggca
    29221 tgagaatttt tcaaatctca tgcctctttt ttcaccctat cgtatcactt tgatacagac
    29281 gagcggtcgt cccactttct tccttgacca ttgtcaaaaa tgtccactcg tatcgttcat
    29341 aaaacgtggt atgatctgta attaagtaaa gtttttcgta cccaagttga tgcatttctt
    29401 ttctgatttc cgctaatatc cgcttggcta tcccctgctt acgcttttcc ggttcaacaa
    29461 acagggcaca aagatttggt gtcagatcag ggcggttatg gaagtcgttt tcaatgacgc
    29521 ccgctcctcc gacgatggtt tctttttcat ctaagaccac ataccattga ggaatacttt
    29581 ccttattagc gattccagta cgaatacttt cctcatacac aacagcagga actccccatt
    29641 ttgatgaaaa ccaattcgcg ctttgtttta ctaattctgg gtgttctctt aatttgatcc
    29701 agttatatgt aaccaatgtt taaacctccg actaaaataa aacgtttaca aaaattatac
    29761 aagtctttat gaaaattagc aatgacaaca atagtgatgt aaaaaaaaaa cggaaaacaa
    29821 tcacttgttc tccgtttttt gattaatcaa gcatctaaag cttcttttct tctttcttgg
    29881 atcgtagcta ccttgacgaa cggtagatag atcaaaatag aaagaaccaa attgacagcg
    29941 gctagtacac cacctgcaat actttttgtt gccaatacac cgccaataat tggtggagtt
    30001 acccatggag gcataacggt agctgctggc actaaacctg tagaagtcgc aaagtaagca
    30061 acactaacta agaccattgg tgtcaaaata aatggaatga acatgatcgg gttcaacacg
    30121 ataggtaacc caaacatcgt tggttcatta atattgaaga cacctggcgc aatcgacaag
    30181 tttgttacaa ccatatatgg tttgttttta cgtcctacta aataaatagc taacagtaaa
    30241 cctaaagttg cacctgttcc tcctaagttg acaaagctgt caaagaaagg tttgttcacg
    30301 ataaacggtg cgctatgccc agctgcgatt gcattaacgt ttgcttcaat ggctggcgca
    30361 ttgattgttt gcatcaaagg atcgaccata ttcgctccgt gtaaaccaaa gaaccataag
    30421 aatggtgtga taaatgcaag cagtaaggca gatggataag agtttgcaag tcccataaat
    30481 ggttcttgga ccactgtata gaaagaacct acgatatcat caacaccgaa tgcagcaaaa
    30541 attgcagcaa ctaaagcaaa cgcagagata gtgatcattg ctggtagtaa tgaagcaaat
    30601 gcttttgcaa ctgccggtgg cacaccatca ggcattttga tgatcaattt ttcatttccg
    30661 cttaaacgta caaagatttc tgttgaaata atcccaacaa taatagcaat aaacaaaccg
    30721 tttgagctca tacctaatgc tccgcctaaa gcgaagaaag agcctaaaga aatcacacct
    30781 gcggaaacac cgtcttttcg gtagccgtta gctaaatgat aagcaagcaa gcaagaaagc
    30841 aatcaagaca gaaagaatgg caaatgtacc gttccaagca gcagccccga atcctttcca
    30901 tgattcacca ccaaaaattg agttcatcaa ttcttggaac ccgggaatcg gtaagttatt
    30961 gatcattact gctaatgctc ctaagatcat taacggcata ctcaccataa atgagtcccg
    31021 aatcgcaact aaatgccgtt gtgcaccaat ttttgatgct acaggtataa aatgtttctc
    31081 cataaatgca acaaatttat ccataaatct tccctctttt ctttactgta gattacgcgt
    31141 tcaatcgacg atacagatca atgatctctt ttgctaaatc tgtaaaagct atagatgtca
    31201 tcagatgatc ttgactgtgg accgtcaaaa gcgtgacttc aacgtgattt ccttgtgctt
    31261 cttgtgtcag catctctgtt tgagagtgat gtgcttggac taaagattcc tctgcatctt
    31321 tgattttttc atctgctaaa gtgaaatcac cttttttcgc agcagcaatc gcttccattg
    31381 catcgctttt tgcatttccg ccaaacatga tcagacccat gatggcttct agattttttt
    31441 gatcttccat ttattgtatc ctccgataat ttttattatt tgtttattag tttttctgct
    31501 tggtcgagta ctttttcacc attcatcatg ccatagtcag ccatattgat tacatctaaa
    31561 ggaatgcctt taggtgctag cttttcagca aattgatttt gcataaagcg tacttgcggt
    31621 cctaaaagta aaacgtctac atttttagat tctagttgat tatccgcatc tgaagcagat
    31681 acagcaaaga tgtctgcttc cattcctttt gcttctgctg ccttttgcat ttttgtgact
    31741 aggagacttg tactcattcc agctgaacat acaagcatga ttgttttctt ttccatttta
    31801 aacacgttcc tttctatact tacacttgta tataagcaag tagcgtgcca actttaaaag
    31861 cgctttaaaa agttgatatc atcacattta aggtctatac caagttgcat acacactaaa
    31921 aaagaaagcg cacactaaac acacaaatta atttttatta ccttttttct tttaacgtgc
    31981 acaaaaaaag ttgtgacaca aaacttgtca caactttttt caaataaacg gtattcaaaa
    32041 gcttgcatct gcggtaataa gtccaaccaa gttaaaaaca taagatgttg tttttacttt
    32101 gctattctta ttacttgatg caggacagct ttttcacaac ctctcaataa acggtgttca
    32161 atcagctgac cctgggtatt agctcaaaac gctggattca caacctcttt tatttcaaat
    32221 aaccagtaaa tacaatgaac atctacaaaa aacttctatt tatttataga tttcgtcagg
    32281 caaatccaat tctctcttat caatcaaagt ctgataccac gaagcagatt ttttcaatgt
    32341 tctgcttcgt tcatcgttca attcgattcg aactaatcca tatcgattct taaatgcatt
    32401 cattggtgac acacagtcag tgaatgccca taacatatag ccttcgcaat ttgcgccttc
    32461 ttctgttgct ttgatcaagt aatgcaaatg ctgtccgata aattcgatac ggtaatcatc
    32521 ttgcacgatt cctttctcat ccatatactg ttcttcatat tctcttccca taccgttttc
    32581 tgttatcagc caagggatat tatgataatt ttcttttaga tacatggcta tatcatacat
    32641 gattttcgga tagatttccc atcccctgga attattcatt ttcttcccag gtaaatcaaa
    32701 gacttcataa tatcgttcag gatggaaagg tgtctcttca ttccattgat aacgaggggc
    32761 gcgaacgcgt ttaggataat attgattgat tccaaggaaa tctactgtat gatgctgaat
    32821 cgtttctaaa tcctctgttg caggatcgaa ataaatcgat tgtttttgac acaactccag
    32881 gaactcttct gtgtagtatc cttttaccat cggatcaaaa aatactcgat tataaaacaa
    32941 atcatacata taagcagctt tttggtcttc tggagaattg gatcgagcat agaccatttc
    33001 aggatttaaa atacagccaa tccttccacc ataatttccg ctgcggtaaa tctctactgc
    33061 tttagccgtt gctaaaactt tatgataatt ccacatcatc cacttttttg tgttttgttc
    33121 atgcggccag cgaacagcat ccaaatagca acgcgtttgg ggaacgattg gttcgttgaa
    33181 tgtaaaccag ttttttaccc tatcaccgta acgatcaaag gcaatttgag catacttagc
    33241 ataaaaatca actacttttc ttgaagacca gccatcatat ttttccaaaa gaaaagctgg
    33301 gacttcataa tgttccaaac aaatcatcgg ttctacgtct gcttcgatca aagagtcgat
    33361 catttcactt atatgctgag catattcttc atctactata cagttctcat aatcagtaaa
    33421 aaaacgagcc caattgatag aagtccgata atgtgtcaga tggatggatt tcatcagatc
    33481 cacatcctgc tggtattgtt ccataaaatt agttgctacg gctgggccat acccattatg
    33541 ccaaacaaaa tgttcatttt tataccagct atctataaac gaatcctgtc cttctttttt
    33601 tcctttccaa ccttccgttt gccaagcaga agatgcagca ccaagaataa aattttttgg
    33661 aatagtaatt ttcatgattt cctcctgttt tttagatccc atcattttcc acacgatttg
    33721 ctacttttac aaatggcaga tagatcaaaa tagaaacaat aatacaaaca agttgcgtaa
    33781 taactgcacc gatatttcct gctgttgcca gataagcatt gacgatcggc ggcattggcc
    33841 aagggaccat tacaactgct ttcgccgcaa atcctaaagc ggttgcaaaa taaccaatcg
    33901 ttccagtgat aagaggcgtt aagataaacg gaatagctaa aattggattc agcatgatcg
    33961 gcataccaaa aataactggt tcattgatgt tgaagatccc aggagcaagg gataacttgg
    34021 tgatttgttt catatcttcc cgttttccaa ctaacaatac tgcaatcagt aaaccgatcg
    34081 tgactccaga cccgcccata ctcatataca tatcccaaaa tggcatagta ataatgttcg
    34141 gtacttcttt tccagcttca aaagcttccg tatttacagc aattgccgct aaaagcaacg
    34201 gttcccgtat cggcttcacc atttgatttc catggatacc aatcacccaa aagacttgcg
    34261 caataaacat caatagcaaa atacccggta acccttgtac gacaccttct aatgggcgtt
    34321 gtacgacatt gtaaatgata tcataaaggt acattcctgt aagttctttg atgataaatc
    34381 ccgcagccgc gatacccgat acagtcagaa tcgtcgggaa taaagcagaa aaactagcag
    34441 aaacattcgt tggcacggta tcaggcattc tcagctgtag tttcttctgt ttcctcaacc
    34501 agcaataaag ttcaatagaa aggatggaga ccaacattcc caaaaataac cctttcgtat
    34561 ctgtatattg tttagccaat acgttttcta ctataacagt ctgattttca ttcactaatt
    34621 cataaacagt tggattaaca gataaataag acatcactgc gatgatacca gggaatacac
    34681 catccacttt attcagcttg gctaattcga tccctatcag aaaaacagca tagatggtca
    34741 agaaacttag tgtgacatag ctgatggcag atgcgatagg ctttaattct tctaaaaaac
    34801 gcaagaaact tatctgtgct aagccatttt ccgagtcaaa gaccattgct gaaaacaatg
    34861 tgccaaaagc acccgtaata attaccggaa tcaatgcagc aaatgcgttt tttatcacca
    34921 tgatatacct taaattattg attctcgttg ccacgttccc taaaacattg gctaatttat
    34981 ccataaaact cattccagat cccctcattc ctgcttcttc tccttgacgt catctctttt
    35041 tgtctattag tcagcgctta catttcgatg aacagaaact atttactcct cttttttacc
    35101 aaatataaaa caaaaattca tctgatgtat ttatcataaa atacatcaga tgaactgtca
    35161 attagtttct ttttctccta ttattttctg taattctctc cgatttattg ccatatggac
    35221 agtcattgca tcaaacgcag caagtgcatt ccgcctttcg atggcctgaa cgatctctgt
    35281 atgaatatcg atcgtctcga ttttcagatg ctggatatca taatttttat taaacaaagc
    35341 aattgattca ttaatgattg ggataatatg cagcaaggct acgtttccgc tagcttctgc
    35401 aatcatcgta tgaaaatcga tgtctaattg aacatgttct tgtgtgcctt ctcgaaaaga
    35461 agtttcaatc ttttgcgtta attttttcat ttggacgatt tgattcttcg ttgcatattc
    35521 cgctgccaaa cctgccatct taggctccaa taaatatctc agttcaaata agtctcgtat
    35581 catccgatcc tgatcttcaa tcaaagaaaa accgagtggg tcattggcaa tccctcgctt
    35641 atcactgaca aatgtccccg ctccttgccg aacttcaagg atattcctgg aagctagagc
    35701 ccgcacagct tctcgcaccg tgcttcgccc cacctctaaa taatcggaca attcatattc
    35761 attcggcagt ttatctccta cttgcaactg catttccttt aaatatctaa ggatcttgtc
    35821 agtagttact tctactaggg ttttattttc ttccaaacca catacctcct tttcatctga
    35881 tgtatttttt cgtaattatt ataacacgtt tgaatcatct gatgattttt tgtatacaaa
    35941 attgattaat ccgttcatag acatacaaac gaagaagata tttaccataa aaaagactag
    36001 gataaaattc actcctagcc ttcgatcatt tttattttga acgacaaaaa atatgatgtt
    36061 caataacaga cgatcttcat tcgattcttg atgtcatctt ttgttttttt taatatatgg
    36121 ataaaaagtt tgattacatc gtttctattc tagaatttgg tcgattctca attcttcttc
    36181 tggtgagaaa tctaaattat ccatcattcg aacactttct tgtaactgac ttaaccgact
    36241 tgctccgacc agtacacttg taacagattt ttgtcgcaaa ttccatgcaa gggccatttg
    36301 cgctaatgat tgtcctcgtg atacggcgat tgtttgcagt gctttgattt tttccagagt
    36361 tgatccaact tgttcttctg aaaggaatgg tatttcctta cgatgtgctc tagaatcttc
    36421 aggaatacca tgtagataac gatttgtcag caacccttga gcaagcggac tgaacgtgat
    36481 tgcccctagc ccttcttctt ctaatactgg agatagatcg tcttctagta gtgcacgaga
    36541 aaacatattg tagcgcatct gatgaataat gaacggtgct tcctttcttt ttaaaatttc
    36601 agtcattttt tttgtatctt ctccattata gttggatata ccaatatata atgcttttcc
    36661 ttgtcttaca agttgcatca aagcttctgc tgtttcttcc aaaggtgtat cagggtctgg
    36721 gcgatgatga tagaagatgt caacataatc tagccccatt ctttggagac tttggtcaca
    36781 acttgaaatg atggatttac gagagcccca ttccccatat ggcccttccc acatatgata
    36841 acctgctttg ctagaaataa tcagttcatc acggtaggct tgaaaatctt ctctaaaaat
    36901 acgcccaaag tttttctctg cagctccagc agggcctcca taattattcg caaggtcgaa
    36961 atgggtaatt cccatgtcga atgcgcctct taaaatctct ctttgattat ggataggatc
    37021 ataatcacca aaattttgcc ataatccgag agataaaatc ggtaatttca aacctgaatg
    37081 accggattta cgatattcca ttttttcata acgtgcacta tctgcaatat acaatttttc
    37141 tcctcctcat caaatctttt taaaacgctt tcttttttct atttttagtt tactacattt
    37201 taaagaaaag gagttaatca aaatgatttt ttcgtatatt tttttgccat ttgtttttta
    37261 ctcgttgata gatcagaaat tgattacgtg cttgctggat ttttttataa cgtttcggtt
    37321 gttttttgta aaattgctga tgatagtttt ctgctggcca aaacgtaccg gctggttcga
    37381 tttttgtgac gattggttgg tggaaagttc ctgaactgtc tagtttctgt tttgattgtt
    37441 ccgctaattc tttttgtcgt tcgccttgat aaaaaatcac tggacgatat tgcttccctc
    37501 tatcttgaaa ttgtccaaat gtatctgtgg gatcagtaat ttgccaatag attgtcagca
    37561 actcgctata acttatttct cttgtgtcat aaatgatctc cacagcttcc acatgacctg
    37621 tgtaaccacc acttacttga tcataatttg gtttttcaac atgtccgccc gtgtatcctg
    37681 acaaaacaga taggatccct tttttcgatt caaacggctc gaccatacac cagaaacatc
    37741 cccctgcaaa tactgcacgt tcttgatagt cagcatcttg catggcatgc ttggaaaagt
    37801 catatttttg ctccttatca aatccttccg taatcaataa ataaaagtcc attacatcag
    37861 gcgtcagatt cattcggata gctaatggac gtagtgttgc ttctagtttt gataactgtt
    37921 ctttaacgga tatattttct cttatgtcat tctttgtctg aatcaatact tttcgttccc
    37981 aatctcgtgt attcggattc aaaatcagat tataaagtgt ttgaatgaat atttgttcct
    38041 catccataga ttctcctcct ttccgaagaa gacaagctag tgactttagc tgtaatttat
    38101 cataaagtga ggaaataagc gattacttca tccccctaca gatttttcgt ataaaaaaga
    38161 gagaatcctt atcgacagat tctccttttt aatcaattta ttttataaat gaaaagttaa
    38221 tttacctttt atggaaaact attttaaaaa ataaacataa atatgcagtt aatgtaacaa
    38281 gataagaaat aatagtcgtt actgtataac ttgtgcttac tttaatatca gcaactcctt
    38341 tgactccgct tatttgtacc aatccattag cattcacctg ctcctttaca ggttgatgat
    38401 ttatttctac attcgttcct aaataagcat atacaggaag tgtgattact tggctcttct
    38461 cattagttac tctatatatg atagaactac cagttgtttt ataaggaagg tttacccatt
    38521 gactgccaat ctttgcttcg tgttttatta tatgctcctt attctccaat gaagcgatag
    38581 gagaataatc atataaaaaa taatccattc ctttagtggt atacgcctct actgtttctt
    38641 tgtttacaac aatatgtccc ggctgagaaa ttattttttg attgaaattt tttttactag
    38701 aaacagttaa agtgattaat aacaaaacag tcataaataa ataaaatagt tctcttgtct
    38761 tattgtatct tctatttttg aaaaatatca tcgttaatat cagagaagag aatattattt
    38821 gcaagcttaa gatccgccaa ggaaattgta acaatttggc tggcgtattt tgcagaagat
    38881 accatggcac caaactagtt gtagctagcc aagtcaaaca cgtaccataa taaatctttt
    38941 tttctatgtt tgttaatttt ttaaataaaa tcaggggaat acataaagat aacagaccaa
    39001 ctagtcccat tgagtaagta gttaagtcgt tgttcatagc ttcagtcaga ctatcgaaca
    39061 cattagatgc atgagcatat aaatttgtca cgctaggttt gttgattttt tggtagagca
    39121 tttgttcaaa catcggatac caaaaagcac tcgtcagaac caatgtcatt cctgccgcac
    39181 taaataattt tttaaatata gcaataaaat ctgcattctt gaaaccttta gaaaccaaac
    39241 gaataaaaat aaaaatgata attaatgctg tactcattaa tgttgatagt acatggctgt
    39301 acactagcaa actcatactc aatgctagct ttacagacgg atacttttct tcttttccag
    39361 agatgatttg ataagcataa aataatacag ggataacaag tgttattgcg atagcttcgg
    39421 caattgcgga acgatagtag atgtctactg tccgataagc agagaaaata tagagacaac
    39481 taaataaaaa tgccgctttt ttttgtttta aaaaccgctc gccataatga taagctacga
    39541 gtgttgtaca caagttcaat aaccaaacgt aaataatata gcttacaatc aaattttttg
    39601 tcatccaata taacattaca gctggaaaaa aagtcagaaa gggataaaaa taatttacac
    39661 catatccagt ataattgaat gtctcataat taatcggact ggtaaaaata gtcgataacc
    39721 ccttgattcg agataagtga aaagaaagat ctccagtggt ataaatactc tgtgtatttt
    39781 tgaaaaagat gaaagaatat atactactaa ctataattaa tccactcaat attactactt
    39841 ttttatttat tttcattctt cttgtttatc tcctttttag tcacatatcg agaatctttc
    39901 aacttattag gtgcaaatcg tttaaaactt gaatacacca ctaaacaata agtcagaata
    39961 ctgatccata aagccatgag agcagttgga gtatattttg ttgtaatttc tatcttattt
    40021 tctccagcat ttacatccac attcactaag ccatgttttt tataagagtc atttacgact
    40081 ttaccgttta cttttacctg tgtacctaaa taagcatata ctggtactgt cactttttgc
    40141 tttttgttgc ttgccactgt gtaagtaatg ttattatctg cagctttata cttccctttt
    40201 tcccacgatt ctcctacttt tacttcatgt ttttctaagt gaggcctttc ttttatcgct
    40261 tctgttgggg cgtaatcgta taatcctccc atatctgatg ttgtataacg tgggagtgtt
    40321 tcttcattga taacaatatg accgtgtttt gattggataa cttttgcgta attttctttt
    40381 gctgctatag acgtgacgag tagaaacacc acagtagcgc caatgattaa ccatgaatat
    40441 tttttattca tagtcttttt cataaaaaca agcgttatga tcattgaaga aaaaaagatt
    40501 tggataccta agatacgcca aggaaactgg agcaagctta tcggtgtttt ttgaaacaaa
    40561 aaccatggaa ataaattagt tacgcatacc catgtagaaa ctacgccaac atagatgatt
    40621 ttttcttttt tctccatttc tttcagtatg aataaaggaa aaatcaacgc aaccaatcct
    40681 aataacccca tagtgtatgt tgtaatatcg ttatttaacg cttgaatgat actatctgaa
    40741 atagaaagtg cttctagctg caaatctgtt ttgaagggac tgtgtatttc ttgataattc
    40801 atctgctgca acataggata ccaaaaatag ctagttaatg cacaagtcaa aaaaacagcc
    40861 tttactaaat tcacgaccac aaattttata tcgtttttta tttgcttgct taaaaacaaa
    40921 cgaactataa tcatacaagc aatcaatgca ctacacaata gtgtagttaa gacatgagta
    40981 tacacaagta gacttatact gacagataaa gcgatgactt ttttttctct attgaatatc
    41041 aatccataag cataatataa aacagctggg ataatcgtaa gagcgattgc ttctgcaatt
    41101 gcggatcgat aatagatatc aactgttcga taagttgcaa aagtatataa acaactaaat
    41161 aaaaaagcag catctatctt tttaaaaaac ttttctccat aataaaaact
//
LOCUS       DACKAC010000017        40939 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.017, whole genome shotgun sequence.
ACCESSION   DACKAC010000017 DACKAC010000000
VERSION     DACKAC010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.017"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            131..574
                     /locus_tag="I0B46_000976"
     CDS             131..574
                     /locus_tag="I0B46_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAR2083292.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(1078..2496)
                     /gene="nhaC"
                     /locus_tag="I0B46_000977"
     CDS             complement(1078..2496)
                     /gene="nhaC"
                     /locus_tag="I0B46_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAR2083293.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(2899..4122)
                     /locus_tag="I0B46_000978"
     CDS             complement(2899..4122)
                     /locus_tag="I0B46_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAR2083294.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(4153..4992)
                     /locus_tag="I0B46_000979"
     CDS             complement(4153..4992)
                     /locus_tag="I0B46_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAR2083295.1"
                     /translation="MKKNIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(5009..5689)
                     /locus_tag="I0B46_000980"
     CDS             complement(5009..5689)
                     /locus_tag="I0B46_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAR2083296.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(5691..6764)
                     /locus_tag="I0B46_000981"
     CDS             complement(5691..6764)
                     /locus_tag="I0B46_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAR2083297.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     gene            complement(7411..8082)
                     /locus_tag="I0B46_000982"
     CDS             complement(7411..8082)
                     /locus_tag="I0B46_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAR2083298.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(8169..10847)
                     /gene="mgtA"
                     /locus_tag="I0B46_000983"
     CDS             complement(8169..10847)
                     /gene="mgtA"
                     /locus_tag="I0B46_000983"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAR2083299.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            11506..11943
                     /gene="ndk"
                     /locus_tag="I0B46_000984"
     CDS             11506..11943
                     /gene="ndk"
                     /locus_tag="I0B46_000984"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAR2083300.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(11965..12831)
                     /locus_tag="I0B46_000985"
     CDS             complement(11965..12831)
                     /locus_tag="I0B46_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAR2083301.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(12991..15084)
                     /locus_tag="I0B46_000986"
     CDS             complement(12991..15084)
                     /locus_tag="I0B46_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAR2083302.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(15833..16441)
                     /locus_tag="I0B46_000987"
     CDS             complement(15833..16441)
                     /locus_tag="I0B46_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083303.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLITAYSEKVDKKVVADRLAEWKKTNEQVKKTTVSSQK
                     NESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(16600..17997)
                     /locus_tag="I0B46_000988"
     CDS             complement(16600..17997)
                     /locus_tag="I0B46_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAR2083304.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(18029..19000)
                     /locus_tag="I0B46_000989"
     CDS             complement(18029..19000)
                     /locus_tag="I0B46_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAR2083305.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            19374..19799
                     /locus_tag="I0B46_000990"
     CDS             19374..19799
                     /locus_tag="I0B46_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083306.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(20287..20988)
                     /locus_tag="I0B46_000991"
     CDS             complement(20287..20988)
                     /locus_tag="I0B46_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAR2083307.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(21004..21444)
                     /locus_tag="I0B46_000992"
     CDS             complement(21004..21444)
                     /locus_tag="I0B46_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAR2083308.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(21444..22019)
                     /locus_tag="I0B46_000993"
     CDS             complement(21444..22019)
                     /locus_tag="I0B46_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAR2083309.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(22867..23346)
                     /locus_tag="I0B46_000994"
                     /pseudo
     CDS             complement(22867..23346)
                     /locus_tag="I0B46_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(23505..24206)
                     /locus_tag="I0B46_000995"
     CDS             complement(23505..24206)
                     /locus_tag="I0B46_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAR2083310.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(24314..25723)
                     /locus_tag="I0B46_000996"
     CDS             complement(24314..25723)
                     /locus_tag="I0B46_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAR2083311.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            complement(25771..27636)
                     /locus_tag="I0B46_000997"
     CDS             complement(25771..27636)
                     /locus_tag="I0B46_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAR2083312.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAEADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHRGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(27757..28608)
                     /locus_tag="I0B46_000998"
     CDS             complement(27757..28608)
                     /locus_tag="I0B46_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAR2083313.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            complement(28918..29631)
                     /locus_tag="I0B46_000999"
     CDS             complement(28918..29631)
                     /locus_tag="I0B46_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAR2083314.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(29699..30025)
                     /locus_tag="I0B46_001000"
     CDS             complement(29699..30025)
                     /locus_tag="I0B46_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAR2083315.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(30148..30375)
                     /locus_tag="I0B46_001001"
     CDS             complement(30148..30375)
                     /locus_tag="I0B46_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAR2083316.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            complement(30697..31950)
                     /locus_tag="I0B46_001002"
     CDS             complement(30697..31950)
                     /locus_tag="I0B46_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAR2083317.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            32011..32751
                     /locus_tag="I0B46_001003"
     CDS             32011..32751
                     /locus_tag="I0B46_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAR2083318.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(32837..33595)
                     /locus_tag="I0B46_001004"
     CDS             complement(32837..33595)
                     /locus_tag="I0B46_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAR2083319.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(33592..34620)
                     /locus_tag="I0B46_001005"
                     /pseudo
     CDS             complement(33592..34620)
                     /locus_tag="I0B46_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
     gene            complement(34640..37807)
                     /locus_tag="I0B46_001006"
     CDS             complement(34640..37807)
                     /locus_tag="I0B46_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083320.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            complement(38031..38915)
                     /locus_tag="I0B46_001007"
     CDS             complement(38031..38915)
                     /locus_tag="I0B46_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAR2083321.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(39010..39447)
                     /locus_tag="I0B46_001008"
     CDS             complement(39010..39447)
                     /locus_tag="I0B46_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083322.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            complement(39664..40575)
                     /locus_tag="I0B46_001009"
     CDS             complement(39664..40575)
                     /locus_tag="I0B46_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAR2083323.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
BASE COUNT        11740 a         8633 c         6875 g        13691 t
ORIGIN      
        1 ttttccgaat aaacagtctc atgacctctt ggtaaacggt gttcaaaaac cctctgtgca
       61 aaataattga ttgttctcca tgctcatgac atgatagaag aaaggaatga aaaaaaggag
      121 tttgttttaa atgaaaaagt attggaagtt tttgttgatg atcggtgttt caacggtagt
      181 tatgtttggt atgatgtact tgaatgtcta tgcgctcgat catatcttct tcagtcgtac
      241 acgagtattc atggcgttga tgatgggtgc agtcatggca gtcatcatgc tgttgttcat
      301 gtggaaaatg tatgaaaaca aaaagataaa tgttatgatc ttagccgcta gtcttcttgt
      361 ttttgccggt tccttgttta tggtacgaag ccaaactact gtcaacgaca cagcttggat
      421 gaaggccatg attcctcatc attctatcgc tatcctggca agtaaacgag ctgatttaaa
      481 agatcctgaa gtaaaaaaat tagcggaaga aatcattcag gcacaagaag aagagatcga
      541 caaaatgaaa aaaatgatca aagaaatgga atgaccatta tccgtccaaa taaaaaaaga
      601 ggatgttcca gaagttatca gcttcaagaa ataagaagaa gctacttctg gaacgccgtg
      661 aataaggggt tgtgaaaaag ctgccctgca tcaagtaata agaacagcca aacaaaaata
      721 gcttctccta tttttcgttt ggttaggctt attaccgcag atgcaagctt ttgaacaccg
      781 tttattaaga ggttgtaaaa tcagcgttct gacctgagta ttagagagac aaatggaaaa
      841 atagttcctc atattttgcc attttttgag ctaataccga gggtcagctg attgaacacc
      901 gtttatcgag aggttgtgaa aaagtcgctt agtttcgcgt attaagaaaa attttgtgaa
      961 atggctttcc acatttttgt ataaatttgg cttaataccg agaaactggc ttttgaatac
     1021 cgtttattcg taaaagggat tgtgacaaaa gtcttgtcac aatccctttt tatgttttta
     1081 ttgttctaat tgcgctgtct tgcttactgc ttcttgcggt ttcacaaatt tttttgagat
     1141 tccccaacct gtgaaggcac tgaccatcga aaacacagga gaaagcaaac tgaaaaatac
     1201 gaatggcaag taagtgagtg taggaatacc aaatgtactt gctacgaaac ttccggcaat
     1261 tccccaaggc actaagtagt tgattactgt tccaccatct tctagcgttc tacctaatac
     1321 agttggatca aatcctgctt tttcataagt ttctttaaat gcattaccag gcagaataac
     1381 agataggaat tgttcaccaa taaaaatatt tactccgata cttgtcatca atgttgtaaa
     1441 aatcaaattt ggtgtgttcg tcatctttct tgatactcgt tccatcacgg ttgagatcaa
     1501 gccaaattcg atcaacaatc cacctaatga caaagttaga ataatcaatg acacagtagg
     1561 catcatactt tcgattcctc cgcggctaag cagtgaatcg atttgttggt ttccagtatt
     1621 tgaaacaaac cctgattcga tgaccccagc caacttcgtt aaggaagttt gtgggttttg
     1681 aataaaaatc attcctactg ctacagcgat attcaacaaa atcgttaaaa ttgccggcac
     1741 ctttttccat gcacacagta acattaaaaa taaaggaatc agtgaccaga ttgaaatagt
     1801 aaagtttgct tctagaacat cagtcacttc acgaatagat gaaagacttg cggctttact
     1861 tgtgtgtcct aaaatcatga ataaaaagaa agaaacaaca aatgccggga cagttgacca
     1921 catcatatgc ttgatgtgtt tgaacaaatc ggcatcaaca atagctgctg ctagatttgt
     1981 tgattcggat aatggagaca tcttatctcc aaaaacggca cctgaaacaa tagctccaac
     2041 aaccagcgct ggatttaagc ctagtgttgt cccaataccg aaaaatgcaa tcccaatcgt
     2101 tgacataaca gtaaaggcac tccctacagc gctacctacg attgcacaca caacaaatac
     2161 agaagggaca aaccactgga cactgatcaa tttgaaccca atgaccatta gagttggaat
     2221 gatccctgct tgtatccata cagcaatcaa tgaaccgact aaaataaaaa taaagattgg
     2281 gatgattcct ggtttgatgc cattgatgat tccttcgtgc agccaatctg tcgggtatct
     2341 ttttatcact gcatacaaca tgaccattcc gatcgctgct aagatcgtga tattaggtga
     2401 gacctttaat ccgacaacgc caatcgcaat gcctaaaaca ataaccatta atacaagtgt
     2461 tgcttctaaa aaacttggtg ttcctttttt tcccatgata atctctcctt ttttagttgt
     2521 tcttatgttc taaaaaagtt aagatatcgg attactccgc cgcaattcat ccgcgattcc
     2581 tcctttataa aaataaaaag acctgtttta aactaaggac gaatgactcc gcggtaccac
     2641 cttaattaaa aacagatctg tttttcactt tcaaactttt attcaaatgt atggactttc
     2701 catgtaattc gtcaattagg cttgatcggt tcacagcgac caccgacttt ctgaacaaaa
     2761 cacctaaccg ctactgcgtg aaaagttgac ttgtacgaag tttaccttcc ataagatcat
     2821 ttgtcaatga aaaagaaaat attttttcat ttttctgaaa attattttta taactttcac
     2881 acacaagttt attttttact agaaagtagc gccctcttgt ttttttcttg ctactatttc
     2941 ttgatgttct tgcttgattc tttctaatag ctttggctct ttcaacagat caagagctgt
     3001 attggcgagt agttctgctg caatcgcaat agaagcaagg cctttgtcac ttttagcagc
     3061 agctttgaat tcttctgtgt gtccggcaat atatgtctca gagattgcta ccgtaggctg
     3121 gattgttggt atgacttggc tgacatttcc tacatcagaa gaaccgagac ttgttctttg
     3181 ttccgtttct atttcttcat ctggaacacc tgcttctttt gcatgctgga agaataagtc
     3241 atcaaaagaa ggagtaacga tgacatcatc aactgcattt tggaacaatc caaactcaaa
     3301 agtcgtgccc gtacttatcg cagcacctcg tacgatattt tccacttttt catatacttt
     3361 atctaatgtt tgccgatttc ctgcacgtaa atagaaacgt ccagcagcgt aatctggaat
     3421 gacgttggca gctactcctc cgtctgtaat gatcccatgg atattgacat cttttggcaa
     3481 ttgcaagcgt agagcgttga ttccattaaa cacttggatc aatgcttcca aagcatttac
     3541 tcctttttca ggtgctgctg ctgcatggga agcgacaccg taaaatttga tgtcgactgg
     3601 atcattcgct aaagattctg tcgttttccc ataacgaaaa gctggatgga cgcataacgc
     3661 tacatcgaca tcctcaaaaa aaccttctcg aacaaagctg cctttagctg atccgttctc
     3721 gcccccttct tcccctgggg tgccatatac acggatttct ccaccagttt catcaatgaa
     3781 ttgcttcact atgctggcgg ctaatacgga gtaagtacca aacaaattat gcccgcatgc
     3841 atgaccaata cctggtaaag catcgaattc tgccaaaaag gcaattgtgg gaccgggttt
     3901 attggaaata taacgtgcat caaaggctgt tcggtgtcca gctacttctt tttctaccca
     3961 aaacctttct ttttctaatt gtttaatcaa cgctgctgag gaataaaact catagttact
     4021 tacttcagga tgttcgtgga tatctagagc aagtgcttga tattctggta aacgatattg
     4081 gattagatcc ttcaattggt ttgttgagat tgttgttgtc ataaaaaatt cctcctatgc
     4141 tagtcatttt tcttattttc tcccgaatgt ttcccatgca ggcacgcttg atccttttga
     4201 tgttttctcg attactttga ctgtgtcttc tgtttgataa gcgtctacta ttttttgata
     4261 cgttttattg ttttcatctt cttttcttgc aacgatgata ttcacataag gacgtgcatg
     4321 gtcatccact ggttctaaga agatggcatc agaagcagga ttaaatccag catctacagc
     4381 tacaccgcta ttgatcactg aaatgtctac atctgacaaa gcacgtgctg tttgggaagc
     4441 atctaattca gaaatgtcta gattttttct attctcggta atatcagaaa cagtgggcgc
     4501 ttgtcctttt gccttatcca ctttgatcaa tccagcagtt tgcaataaca gaagagctct
     4561 gccgccattt gtaacatcat tagggattgc tacttttgct ccgtctttga tatcagctac
     4621 atccttcaaa gtgttagaat aaattcccag cggtgcattg acagtatttc cgatcgatac
     4681 taaatcagtt ccgtgttctt tattgtaatt gtctaaaaag aattgatgtt ggaaagaatt
     4741 caaatcaatc gatccatctg ctaaagccac atttggttga ttgtaatctg taaatttcac
     4801 gtattccaaa ttaatgttct cctttttcaa acggtctttc acgttgtccc atacatctgt
     4861 atcttctcca ataatcccta gttttacgat agtttctttt ttgtcagttg tatctgcttt
     4921 tccttggcta caccctgcca accaaataat tgctacaaat gtaatgacta caaaacgaat
     4981 attttttttc attttttctc ctccactttc aatgtgtaat ttttttaatc agttgattac
     5041 tgataaattg acttaaaaaa actaagatta gaatcaataa tgtagccaca atcgttacat
     5101 ctgtttggaa tcgattatac cctctcgata tagcaagatt ccctaatccg cctgcaccta
     5161 ctgctccagc catggtagtc agtccgatca cgttgatgat agtcaacgcc gagacccgaa
     5221 tgataccagg caaaccttct aacaaataca cacgtaaaat gataccgatt ggactggttc
     5281 ccatcgcttc tgccgcttcg atcaccccag gatcaacttc tagcagtgcg ttctcgattt
     5341 gacgagcaaa aaacggaaca actccaaaaa tcaatggcac taaagctgct ttcgcaccaa
     5401 tcgtcgttcc aactaagaaa cgagtagtca atgctaaaag agaaagcaag ataatgaaag
     5461 ggatggaacg aatgatattg atcagcttat caagtatttc aaatagcaga cggttagcta
     5521 gtaatccatt agggcgtgtc actactaacg tcacacctaa gatgattcca atcatcccgc
     5581 caatgaatgc tgtccaaaaa gacatatata gtgtttgctt cgtcgcttcg acaaattcat
     5641 ccggaatcaa ataaacattg ggcaaatatg ttttaaagaa atcagtcatt ttatcttcct
     5701 ccttcgatca ctttgatacg actatctaaa tcgttttttt gtaaaatatt tatttttaca
     5761 ccagatttga tcaaataatc caatgctttt ttccgctgtt tggtttctcc agctaaagta
     5821 acgatcaaac tccctaacgg cgtttcttgt aataactcta cattgccata taaaatattc
     5881 gctgttactt gatatttgct gtacagatga gcgattagag gttcgttcgt ctgatttcca
     5941 atataactta attccaccag ccatttattg gcgatctgat cggcaaaagc agaatgggca
     6001 atgatcgttt ctaatgcttg gtcaagatga gtagctgttc gaataaaatc tttggtcaat
     6061 tcttcttcag gattgctgaa gatctggatg ctgctacctt tttcaacgat tcttccatct
     6121 tccataaccg ctaccttgtt gcagatttct ttgacaactt gcatctcatg agtgatcagg
     6181 acgattgtta aacctaactg gcgattcaat ttttttaaca atgcaaggat ctgaagggtc
     6241 gttttcggat caagtgcact ggttgcttca tcacataata ataccttagg ttcattcgcc
     6301 aaagctcgtg cgattgctac tcgttgtttt tgtccccccg acagttgttt aggataagca
     6361 gatgcttttt cttccaaacc gacaagttcc agtagttcat tcactttttg acgacgttcc
     6421 tgtttcgatt ttccagaata ctttaaagaa aaatcaacgt tgtcaaaaat agttcggctt
     6481 tccattagat tgaaatgctg aaagatcata ccgatggcct ttctttcttt tcgcaattct
     6541 ttgggagaga gctgactcag attcgtctga ttgacaatga cttctcctgc tgttggtttc
     6601 tgcagcaaat tcatcacacg aactaacgta cttttccctg cacctgagta accgacgatt
     6661 ccataaacat cgcctttttc aatcgttaaa tctacagaat ctactgcctg tatctctttt
     6721 tctttttgtt tgaaacatac agaaacatct cgcaattcaa tcattaaaat tcctccttat
     6781 ctaaaaaaag cgtcctcaag aaaatgaaaa tgtcattttc ttgaggacgc ttttttgcgt
     6841 gttaccacct catgttcaag ctgtgttcac acacaactcc tcttcaagta cgatcatact
     6901 ctagcaagat atcggtcgca gccgtcatcg ccttacatct atgctcggga tgaattgctc
     6961 gatggccatt ttccaatagt cgttccttgc ttcttctcag ctaccgaagc tctctgtcaa
     7021 aggttcttct atcgtactta ccatttcaat gcaaataata ttcaaaaaaa aagtcctttt
     7081 actaacataa actatgtcag taaaaggacg acgtatcgcg gtaccacctt ttttcggaag
     7141 tttccttccc tcaagcccaa catctcaaaa agagagattg gcgttcgttt taacggtcga
     7201 acagttccga atttgcctac ttatcgacaa attattctcc aaggccattt tcagcttttc
     7261 ttacttgact cttctcacca gccagagcct ctctgcaaag tttccaaact tactttcctt
     7321 ttcaacgaaa tcgatatttt gttgttggga ttaatctaca tgaggaaaac tgaaaagtca
     7381 agtacgaaat tcaaattatt ttttctatat ttatttttct gtttttttat ttaacatttc
     7441 aataacagtt cgaagatctt ttccttccgc aagaagtttc aatttttcct tttcttcgac
     7501 ggtataattt cggacaaaac tggaatcagg tatcaaaaca acgcctaatc ctgctcgtga
     7561 agcagctgtt gctcctacta aagaatcttc cactgcaaag gctgaactag cttctaatcc
     7621 tgttcgttcc aaagctgtca gataaggaat cggtgaaggc ttgttctccg acacttcgtc
     7681 tccgaatgta aacgtgtcca agtaatcagc taatttaaag tgatccaaga tatctgttgc
     7741 tcttttcgta acagtggtcg tagcaatgcc tagtttcata tgacgttctt ttaagtctgc
     7801 caatatttct tttgcatatg gttttaagtg gatggctccg cttgtcagct gttgataaaa
     7861 atattcttca cgcttcgctc ggacttcctg tactttttca gggctacctg ctgctttcac
     7921 taaaatatca aaactttgtt tccagttttt tccgccccaa gcgtcaacga tttcttttgg
     7981 gatatggata tcaaattgct gaaatcccca aagccagcca tcacgatacg ttttttctgt
     8041 gtcaactaac aaaccatcta aatcaaatat tgcacctttc atctatacat taatcctttc
     8101 caaaacaata cacatagttt agcacgactg gtccaaaaag aacagaagca aagaaccagc
     8161 cttctcgttt ataagagttc ttggaatcga cggatataga ttttcttcac agctgtcaca
     8221 agcgccagat aagcgatgac tgtagcaaat aaccataacc agtaattgcc aggaagtggc
     8281 atcaagccta attcagcacc aaaacttgta aaaggaagga cagaaccgac aataattcct
     8341 aatgtagtga taacaaacat ggcaaatgtt gcgttgcttt ggataaatgg tactttcggt
     8401 gtacgcagtg catggagcac taaggtttgt gtccatagtg attcgacaaa ccatcctgca
     8461 tggaataatg cgatgaaaat cactcgctgt gaactatcca atgtatggaa acttccgccc
     8521 actacagcag gacaaataac aaaatacatc aacaagtaag tagtaatatc aaatatcgaa
     8581 cttgttggac caaagtaaac catgaattcc ccaatactag atgcattcca tttttttggc
     8641 tcgtccaaat attctttatc catccgatcc catggcaagg aaatgcagga aacatcatag
     8701 atcagattca aaaacaaaag ttgtaaaggc agcattggta aaaatggcag gaacgtactt
     8761 gcaatcagca cggaaaacat attgccaaaa ttagaacttg ctgtagcctt gatatatttc
     8821 atgatattcc caaaagtttc tcgtccagaa agaaggcctc tttccaaaat cgtcaaatct
     8881 ttttctaaaa ggatcacgtc tgcagattct ttcgcaatat ccacagctgt atctacagaa
     8941 ataccgatat cagccacttt cattgcagga gcgtcattga taccatctcc taaaaatccg
     9001 acagtatgac catttttacg taaagctgtc gtcaaacgtg ctttttgctg aggattcaat
     9061 tttacgaata cttcatggtc ttcagctact tgtgctaatt catgatcact catttccgag
     9121 attttctcac cggtaaccaa ttcattgatc tctagaccta cttgacggca cactgaacgt
     9181 gtaactaatt cgttgtcccc agtcagaact tttacagcta caccatgttc tttcagagct
     9241 ttcaacgctt gtttcgttgt ttcttttggt ggatcaagga aagcaagata accaatcaaa
     9301 accatgtctg actcatcttt gactgaaaat tctccaacga cgcttggatt ggtcttttgt
     9361 gcgacaccaa tcactcgtaa gccatcttca ttaagtgact gtacttttgc aagtacggct
     9421 tcttttcgtt cttttgttaa tggtgtaaca gtaccgccaa gatcaataaa tttagaaatg
     9481 ttcagcattt cttcaatcgc gccttttgtg atcatttgtg ttttaccagc tggatcagca
     9541 accacaacac tcatacgacg acgttcaaaa tcaaaaggaa tttcatccac tttttgataa
     9601 ttgatcttat ctgtatctaa agtttctgtt gcttcatcaa tgatagccac atctaacaaa
     9661 tttttcagcc ctgtttgata gtagctattt agatacgcat ggcgcaaaac acgatcatct
     9721 tcatttcctt cacaatccat atgatattca agaatgattt tatcttgtgt caatgtcccc
     9781 gttttatccg tacataacac atcgattgca ccaaagttct ggatggcatt caaatttttg
     9841 atcactgttc cttttttggc catcacactt gctcctttga ccaaattcgt cgtaacgatc
     9901 attggaagca tttcaggtgt tagaccaact gctacagata gtccaaaaag aaaagcttct
     9961 agccaatccc cttttgtaag cccattgatg acgataactg ctggagccat caatgacata
    10021 aatttgatca gtagcatgga tgttttatgg atgcctaatt caaagcttga ttgaattggt
    10081 ttttcagata gcgtttctgc tacatgacca aatagtgttt catttccaac agaaacaatg
    10141 attccttccg cacttccact gatgacatta cttcccataa agacaagatt ttcgtaactt
    10201 gtttcgcttt gctgatcgtc ttcacatact ttcgcatgct tttctactgg atagctttcc
    10261 cctgtcaatg ctgcttggga aatgaataaa tcctttgcgg acaataaacg catatccgct
    10321 ggtatcatat cccctgctga aagccgaaca agatctccac agacgatttc ttccatagga
    10381 atctctgtgt actttccatt acgttttaca gctgccgtta ctttcacaag tgatttcagt
    10441 ttttcagccg cttgattgga acgtacggac tgaatcaacg tcattgtccc gctgacgata
    10501 accatagtga agataatggc tacaccgaaa agatctttat ctccaggagc tgctaataca
    10561 tattccgtga tgaatgaaat aattcctaat gcgatcaaca ctaatgtaaa tggcgtgatg
    10621 tatgctttca atatttctac aatgaatggt gtttttttgc catatgaaat cgcattttca
    10681 ccgtaatttt ctcttgaacg ttcgatttgt tcttctgtca atccatttgc tgatgcatgg
    10741 aatagttcaa ataattcttg tttcgttttt ccagcaaaag ctgcatagat tttttgtgta
    10801 tctttctttt tttccatctc aatctctcct ttgtgtccaa aagacagctg ttctcgctgc
    10861 ttttggtctt tagctattca tcggatcgat agagaaaaaa ctgaaagggg ctgagaacag
    10921 atagataata gccctggcct ttcctctaga tccttgtttt tcgaatgact gtcatccatt
    10981 tgttctcatc tcctttacta agtaatttta attcatgact tccaatcatt tttcgaatag
    11041 ttatttttcc aattagacca cctccttttt atttttcacc gatggggatt tgcggcaaca
    11101 aaaaaacctc tcgccaaata ctcagcgaaa ggtttgaaaa agcatattaa aaaaagcttc
    11161 tcagatctca tcgttgagtt ttgacactgt acaacgtaaa aagcagatac ctgcttttag
    11221 ctaccttatg aaaagcctta tctcgacaat tcctgttcta cccattggcg tctctcgacg
    11281 tttttgggca gtagcctatg tttgtatagg agcctcacct aacaaatctt attcggtttt
    11341 ccttcgttaa tataacaact aaaaaagagg atgtcaaatc aattttccat gtttttcata
    11401 ctttttttaa aatacacacg ttatattcct gtctcacgcg gttttatttg tctcattttc
    11461 cacctcccgc tataatagaa atatagtagg aaacgaggtg ctgttatgga aagaacactt
    11521 gttattatca agccagacgg cgtgagacgc catttagtcg gaagcatcat ccaacgtttt
    11581 gaagcaaagg gactggccat tgctgaaatg aagtttgaaa ctatgactcc tgaattagca
    11641 aaagaacatt atcagcactt gactgagcgc agtttttttg atgaactgat cgattacatg
    11701 acttccggtc ctgtcgttta tcttgtgctg gtcggcgagg aagtgatcga tatcgtccgt
    11761 aagatggttg gagcaacaaa agctgccgat gctgttccag gtacgatccg aggagactac
    11821 gcgctgccgg ggactgaaaa catcattcac gcttctgatt cccgtgatgc tgcggtaaaa
    11881 gagatcgccc gctttttccc gccattcgat ataaacaaac cgtctgaaaa agcggcgaag
    11941 tgatacaaat tctaaactgc tggtctagtt gctgggttca gcaatggcca gcagtttttt
    12001 ctctaaggac ctccgttgat tctttcctaa catatattcg agcactgtga agaattcttg
    12061 gtagtctttc gtcttttctt cccctaaagc agcagaaatc aatctttgat aataatctgc
    12121 catcaatgtt tcatagatca ctgtacttct ttctttatgt gctaagctcc gtttcatatc
    12181 ttcattcaca ctacgtgcct ttttcaaccg accattttct atataaaacg aagtcaggtt
    12241 cattaaaaaa gtaaagagat actgctcatg attgttcaac tgtgctaatc gtcgcattct
    12301 ttttttgatt ttcaaataat tgttctcaat gaaccgatca ctgaatatcc aaggaaaatc
    12361 agctagaata gatagttcaa attgaccata gtctggtatc tgcatcaagt attcttcaat
    12421 gattcggcat tcataacgta aaaattcaaa ttctgtctca gatacttctg ttttttcgac
    12481 catgtgaaga ttcaaaacca aataatacag atcttctgtt ttcttaaaat gcctacggtt
    12541 attgctgatc tctttataag gatcaaagct gtcttcttgt acccgatcta gcattcgtct
    12601 ttttttcttt aactttattg gttcatccag ctggtttcgc atccattcat attctgcaag
    12661 tgtcatgttc attcgctcta aaaatgatct caatgttaca aatgacattt ttttcactga
    12721 atttgtctca atcccgacta gagaagtctt aaaacagatt ccttccgcta attgggactg
    12781 tgtcaacttc ttgttgactc gaagtttctt gatcaattca ccatcataca taatagttct
    12841 cctttttaat ttaaattttt ccacattcaa taaatagata cgttaaaaag gaaaaataca
    12901 cgaaaaaaat aaaaaaatgt ttttttcata aaaaatacca accttcaagc attagaatgt
    12961 tgtctaacta ctgaaggttg gtttgattgt ttattgatta tttttttgct gttcaacgtc
    13021 tgttcttgtt actggatata agttggttgt tgttttatta ccattttttg aaaagacact
    13081 tgtcgaatgt tcgccttttt gcttttcgat tgcttcatta cgttccgctt catttttata
    13141 gtcatatttt ttcggatcaa tcgtctcaaa tccgtttggc acataaaacc gcaagagatt
    13201 ttcctgattc agcttatctg aaagacttaa cgcagtcttg actttttcct gatctttgga
    13261 gatttcctca tcgattcctg ctgctttcgt atcgatgatt tctcctgtct gattattgta
    13321 agcttttcct cctaggacgg tgtatttggg tgtcacaaag ttcccatttc gaaaagcaac
    13381 tacttgatca tgctgagaag ataataagtc tgtaccaaaa tgaagataat ctttatcatc
    13441 gattcccaat agatgtagta atgtcggcaa aacatcgatt tctccaccgt attcatgatt
    13501 gactgtccct ttagtataac caggcatatg gatcatcaat ggcactcgtt gcatctgtgc
    13561 gttgtcaaat tcatcccatg tatctggatc tttaccaaga gcgcttgcta aatctttatt
    13621 atctgtattc gagatcccaa aatgatcccc gtaaatcaca aagattgaat tttggtaaat
    13681 accagatttt ttcaaatacg taaaaaattg ttccaatgat tgatccagat aatgtgccgt
    13741 tcgaacataa ttatctactg tgctattgcc agtattcaaa gatggaaagt cgaaattctt
    13801 gtcatccgta taataaggcg tgtggttggt tacggaaaga aatttcgtgt aaaacggctg
    13861 ttgtagatgc tcaagatatt gggcagattc tctgaataaa tctttatcta atatcccata
    13921 acctaacgtt tcgtcggatt catcaaaata agatcgatca aagaaattgt cataacctaa
    13981 gtttttatat acatgatctc tattccaaaa actagctaca ttcccatgga aaacagcgct
    14041 tgtatagcct tgcttttgtc caaggatcgc tggtgcggct tgaaagacat tgtctgaccc
    14101 aagttctgtg aataatgagc cttgaggtaa accaaatgtg ccagtttcca acatattttc
    14161 agcatcactt gtctttccct gtcctacttc atgaaagaaa ttgtcaaagc tgatcgttgc
    14221 ttgattctgg aaaatactat ttaaaaacgg tgtgacttct tgtccgtcta ccttcatatt
    14281 aatcaaaaac tgctggaaac tttctaaatg gagaacgatc acatttttcc ctttagcgat
    14341 cccaaatgtt tcgggatttg gctctgcata atgtttcttt gtataatcta aaacttcatc
    14401 aatcccatta ctgcttgcat gcgcgcgaac actgctagtc attcctgttt tgattccgtc
    14461 ataaatagta tacgcatcga ttcctagata tttgacaata tatgaacggt caaatgttcg
    14521 ctgcagcaat tgtggacggt tggcttcgct cagcgctaga tttcctgaaa aaataagccc
    14581 tgaaagacag gtaacagcga tagccatcgg tttgccgaca gggttgcttt tgataggtat
    14641 tttcttgacc ttcaaccaaa ccagtagccc aatgaacaca gcgatatcca gccagtagat
    14701 gacatcatgg ggcttcatca aagaaaagga gcttcctgac aatccttgcg acactttcga
    14761 aaagcctaat acagatttga ctgtaatgaa atctgtgaat tcccgataaa aaataatatt
    14821 caagtacaac aaaagcgtat ttaaaatatc aatgatcaaa agaatagcta acgctggctt
    14881 tggcttttta atatacaaag aaaggccaaa aaacaataag gtagtcgcaa ttgggttgat
    14941 ccacaagata aagtactgga tcgtaccttc cacacctaat gaaaaatctg catagtaggc
    15001 aatgatcgtc ttcagccaga aaaagaaaac agctgtcaaa acgattgcta aacgattttg
    15061 gcacaatttt ttaagttgtg tcacgtatat cacttctctc tattcacatt ttttcttatt
    15121 ttgctcctga aaaatgaaaa tttcctaaaa aagttgttat cttaagattt ttttattctt
    15181 tctaaagatt attccgtcca cttttcacag cataaaaccg ctgcgatgta agcgattaaa
    15241 ctaatgaaga gataaaagtc ttgtggtgac cgcttcactc cacggtattc aaaagccagt
    15301 ttctcggtat tcgtacaaaa aaagaggctg tgacaaaatt cttgtcgcag ctccttattt
    15361 cttgatgcag gacagctttt tcacaacttc ttccttaaac ggtgttctcc tgccaactcc
    15421 tcggccacaa gtcacaatat ttataaaaca tgagaagctg ttttttgaaa ttgttccttg
    15481 tggctcagag ttaaacggca ggctcaagac ctcttgatac acggtgttca atcagctgac
    15541 cctcggtatt agattatgtc aggacgctaa ttttacaacc tcttggtaaa cggtgttcaa
    15601 aagcttgcat ctgcggtaat aagcccaacc aaacgaaaaa tataaggagc tattttttga
    15661 aattgctcct tgtggctcag agttaaacgg caggctcaag acctcttgat acacggtgtt
    15721 caatcagctg accttcggta ttagctcaaa aaacggcaaa atatgaggaa ctattttgcc
    15781 gtttgtttct ctaatactca ggtcaaaacg ctgatttcac aacctctgtt tttcattgtc
    15841 cataggattg atattgtcct tgtgatgact cattcgtaat aattgaggct tgattttctt
    15901 cacttcccgt tatcgtttgt ccactgccag aatctgaagg acttgttcct gtcgactcgt
    15961 ttttttgaga agagacagtt gttttcttga cttgttcatt tgtctttttc cattccgcta
    16021 aacgatcggc gaccactttt ttgtctactt tttcagaata agcggtgatc aaacgggcta
    16081 ctttgtcgcc atcatgagtc aaaagcaaaa cggtcaaatg cttgtcttta aattgatatt
    16141 gtctgagaga aatcgcacta agtacgtcat cgatattaat aaattgattt tcttcttttc
    16201 cttttggcaa ataaatgtcc aattcgcctt tttcgtaatt ggcagttatt ttatactccg
    16261 cccaccaata agttgagaat tcttttttga aattatctac tgattctttg cttgctacga
    16321 ttttttctgt ttctttctga gcaagatctg ctgtcatttt tcctgaaaat gctgcggata
    16381 aaataagcag taagaaaaaa ccgaataacc aaaagagaag aaacggtctt tttttcctca
    16441 tgctttcctc cactcctatt taattttgac tattctatta tatacaaaaa atgaaataga
    16501 aagacaaaaa agttgaaaaa gatcgtaaat aattgaactt ctttttcaca aaatggtaag
    16561 aatggagaat ttcacgcatt tgctctcaac tattctttct taagcctcaa atccgttttt
    16621 atctgatacg tctttgaacc aatgacccga ttttttgatt gttctttttc catcttgtgc
    16681 taagtctact gaaataaagc catatcgatt cttatatgca ttggtccacg accagttatc
    16741 catacatgtc cacatatgat agcctaaaca attggcacct tcttgaatgg cttgatggac
    16801 atatttcaga tgagattgga caaattcgat ccgataatca tcgtggatca tgccatcttc
    16861 tttgataaat cgtgcttcgt tttctacacc cataccgttt tcagaaataa agcagtcgat
    16921 atttccataa tgatctcgta cattgatcaa aatatcatag attccttgtt catagatctc
    16981 ccatccgcga taaggattca ttttacgacc aggcatttca taattgtcga aataatcctc
    17041 aggcattgga ccgtttttag tatcgattgg tgtttctttt gcttttaccc ggcgtggctg
    17101 gtaataattg atacctaaaa gatctactgt attttctttg ataaccgtca aatcctctgc
    17161 atggatcaca ggcatgagat caagttcttt tactatctca atcaattcac ttggaaactc
    17221 gccttttaca gctggatcaa ggaatgaacg attgaagaac gcatctgcta tttgcgctgc
    17281 tttaacatct tcagggtttg attcgtctct tggatagctt ggtgtcagat tcaagatgat
    17341 accaataaca ccaccttgct ccatttcatg gtaagatttg atggctaacg ctgaagccaa
    17401 cgcttcatga tagccaactt ggatcgcttt tttcatatct gactcttctg gataatgcca
    17461 tcgatataaa taacctgctt cgactggaac gattggttca ttatgagtaa accatttttt
    17521 tactttcgaa ccaaacaatt caaaacaact tttggcatat ttttcatagg cttcaaccgt
    17581 ttctcggttc aaccatccgc ctttttcttg cagatacatc ggcatatcga aatgatacag
    17641 attcacaaag ggttcgatac cgttttcaat caactcatca aaataacgat gatagaaacg
    17701 aacggcttct tcgttgattg ttccagattc atcaggtatc aaacggctcc attggataga
    17761 tgttcgaaat gaattatgcc cagtctgttt catcaactgt acatcctctt gatacttcgt
    17821 ataaacttgc gaggtttttt caggtcctac ttgatcaaag aacttttgcg gttctttttt
    17881 gtaccaataa tcaaaaatgt tctctccttt tccatcgcct gaaatccttc cttctgtttg
    17941 tgggccactt gctgcagaac cccaccaaaa atttgctgga aattgatatt ttttcatcaa
    18001 agcacctctt tttctttttt agttcgtttt agttgattcc atgtatgata ttttccatca
    18061 caatcacttt ttcattttct tcctcttctt ttttgattcg ttttaacagc tgttctgctg
    18121 ctcgttcccc gattttatct gttggtggcg tgatcgtgat tttcggaagg atactcagat
    18181 cgtttttatc taaataatca tagtgactga acgcaatatc cttcccgatt ctgacacctt
    18241 cttgattgaa acattcgatg gcaccaatcg acatattgta attgctgaca aaaatagcag
    18301 ttgccccttt ttctaataat gcttttgccc cgtaatagcc gctttttcta gaatagtctc
    18361 ctgtaaagac ctctaattgg atcgatggat cattttggac cgcttctttg gctcctgcta
    18421 aacgttctct tgctacgtag tctgtttgag tggcagcaat aaatccgatt tttttatgtc
    18481 cttgttcacc atgatgagcg atcacttttg ctacactttc tcgatcattc actaatattg
    18541 tatcaatacc tgctggtcct tctggacaat tcaatgcgat aactggaata ctcatttttg
    18601 tgagcatttc aaatccagac cactcttctt cggataagaa gacaatcagc ccatcgatca
    18661 catgcttcat caggtattca attttttgtt caattttttc tggctgatcg gcaaacccgc
    18721 ttaataataa gctataccca ttttcttcag ctactctttc gatccctgag acaacttccg
    18781 acccaaaacg gctggaaatc ccgtttatca aaagtccaat agagaagctt cgattattct
    18841 tcaacccttt agcaatgatg ttttctttat agtccaacgc tgaaatcgct tctgctattt
    18901 ttttcatgtt cttttccttc agttgctgac cattcaagta acgggaaact gttcccactg
    18961 aaacaccagc catcttagcc acatcacgga tcgatgccat acattctcac tcctgccagt
    19021 tacatttgat ttctctttct tgttgttcga ttatgatgac cgcattcttt gcatagtgtt
    19081 gcttgtcatc aatatccata taactaaagt cttcagtaga gcaacttcct ctcactatta
    19141 attatactta aacaagtatt tgaaccggtt catttttaat atagcatttt tttaaaagat
    19201 tgcaacactt ttttaaaacg ctttctgaaa gtggcggatt ttttgccttt ttaaaatttt
    19261 attctaaaat ttactctatt tgtgggtgtt ttgtgcgttt ttctcttcca tccgtttgaa
    19321 gttttacatt ccaatggtat aatttcttta tcctatcaaa tgggggaagt attttgaaga
    19381 aattaatcgt actatttatt ttgtctgcgg gacttttgac attgactggc tgtgatttgt
    19441 ttggcggctc tgaccaatcc tcggacgaaa caaccgattc ttctgtctta aaagcggaaa
    19501 aagcagatac caccaacagt gtatatacgc taaaagtacg taaagatatg gatttatccg
    19561 cgggacagtt gacatttaca gataaaaaaa tggaatggac acgcacgtat cagacacaag
    19621 gaactgattc ttctgaatca tctgaagaaa aaacagtgct gacagatatc aagatcacta
    19681 ccaaagatga cgaatatatt atttcaggaa aagaaaataa aaaagaaaca acagtcatat
    19741 ttacaaaaat cggtaattac cgtttgaagg atgaggatgg caacatctac agtctatagt
    19801 gccgtctgtt ggatcttctt ttgtttctcc ccttcttttc tgctatagta aagaagcaag
    19861 atgattgtaa aggagacgaa atggatgatc gatagtcctg attattgtac atgtaataac
    19921 aacaaaaata tccatacaga atttaatgac caaggaagct tgagcatatg cgacaactgt
    19981 cacaaactag tcaaagattc atttaaaaca tttgaatctt atcctgttca aaattatgta
    20041 aaaagtagaa gaagataaac aaatccaagc aaaactcact atggctgatt tttatgaact
    20101 tcaaactcag cttcttcctt tgatcaagct tcatcagtaa aacaaacagt ggatcatttc
    20161 agtttacaaa tcaaagagta aagatttatt tatttcaaat atttgaatat aaaataacca
    20221 aacgatagaa aggcttattc tacatcacgt agtaattttt ctttcccgtc gtttggtttt
    20281 ttatttttag cctaattctt gaccttttgc atgctggtta tccgtaaaat attttccagc
    20341 aggctttcct tccaattcag ctaatttttc ttggatctga cggatcacct tttttgcatc
    20401 ttctggattt tctacaaaat cgaatttatc cccatccact tgtattttag gacatacatc
    20461 aaaatcttca taccattgat tatagcgggt gttcaacgtt tcataatagg tatataacgt
    20521 ctcatcgctt tctagctgtt catagtcacg cccacgtttt ttgatacgtt ctagcatcgt
    20581 gtccaaagaa actttgatat ggaccatcaa atcaggccgt ttttttggag caacatcatc
    20641 taactcgttt agcatcgtat ctaataagct atcgtactgc tgtacttcga tatccgttac
    20701 ccgacctaaa tcagcgttta gatggaacaa caggctatct tcatagatgg atcggtctag
    20761 cacattgtca tcgttcacta aagcattttt cattgctaaa aatcgcttgt tcaaaaagaa
    20821 gatttgcaat aaaaaagcgt actgctcagg atttgaataa aagagcggca atacttcgtt
    20881 atcttcgaca ttttcataaa atggtttaga atcaatctct tttgcaagca tatccgtcaa
    20941 actgctctta cctgcaccaa tcgttcctga aatcaaaatc actgacatac aacccttcct
    21001 cttctataag tactcgccta cgtaagtttc aacaggcaat gtttcaaaat catattcacg
    21061 tacagcttgg tctgatttta aatacgtatg gagactttgt gtaatcatca agttcgtagg
    21121 taccgtctct tcttgtacaa tgatcatcgg ttttttcaac atagttgcca cacctaattc
    21181 gtaagccgtt cctggatcga ttgtttgatg ctcaaagtca aggatagcaa caacaaattc
    21241 tgcatttgtc agttcttcca tatctttttc gtatacttcc tgcgcccatc cagcgctgaa
    21301 tagttcataa ttgctttctt ggtgatgacg tggagagtag aaacttgtga ctgttttgtt
    21361 ttcctccaac gctttttcga tacgacttac tcgatcgatt tgctcctctg aaaaaaatgg
    21421 tcctgctaaa tatatttgac tcattatttt gcctccttca ataataactg atttttaata
    21481 gatactgctc gtaattcatt gaccagttcc aatggattgt tggaaaagat tacttgatcc
    21541 aaccattttt tatccataaa atcctctttt gcaaaagttt ctaataattg ttgcaagccg
    21601 ctgtaataac cgccaatatt caatattccg attggcttgt cgggcgtcag accgattgct
    21661 atccaagaaa gaagttgtga gaattcctcc aatgtaccta gacctccagg gaaaacgagg
    21721 acagcatctc ccttttcaaa taataatacc tttctttcat cgatcgtttc tgtaggaata
    21781 aatgtagtaa catcttttcc aggcaattca gtttcaaaaa gcccattcgg atagatacct
    21841 gtgacttttc cgttattttc cagtactgtt ttactaatca ctcccataat cccggagtat
    21901 gaaccaccgt aaaccagttc gatttcttct tgtgccatgt attttcctaa cgtttgcgca
    21961 ataaatttgt aactttcttt attaccaaaa cgggaaccgc aaaaaacagt aagtttcata
    22021 aaaaaacact ccctaactca aaagtctatc ttcattctac acgtgttttt ttaaaataaa
    22081 agactagatt ttgtagtttt ttagtggtat ataggataga cgccactata tatagtattt
    22141 agaaaaaaat catttttcag ccattttata gataaaacaa aaataaataa cgtgttttca
    22201 tcgatatgaa atgacttact tatagaagat actgggattt cgtcctatat gccaataacg
    22261 tgtattattt taaacaacaa atacttgttt cttttattaa cgaaaaaatc attttgttct
    22321 cgtgttatgt ttcactgata aatgatgttt ctctaaataa aaaagccgac gaactaaacg
    22381 atcaaaattt gatcattttt gttcatcagc ttctcctaaa aatctttttg ttaacgataa
    22441 atagctggat ttgtaaaaat atcacttgaa tcgtcactgc tggaagaaaa ttcagtgata
    22501 accgcacctg ttgcgcctgc ctggaactag tgtttgatat tgggctcgaa cgtgtactga
    22561 tctctagcat gaagaataat ttcttttttt actgtataat aactttcttt cctttttgga
    22621 atagtacagg aaagtttttt tgtttccttt ccttcgacat aaggtatacc agaccctttc
    22681 gacaacgaaa ggttctcatt tttccttcat cgtcatttca aggagggtgc cgatgctctg
    22741 gacagtttcg atcgctccta aaccaaaatc tgtatattcg attctttcta actctgattc
    22801 tttcatacca ttcctgtctc aaggtacatc tattgtactt ttttcttgta atccattctg
    22861 cctctcctaa ctaattgtca aatcatccca agatatgtct tcttcctctt tttcttcttg
    22921 gatgatttct gcattcggat ctactggttt tttcaataat aaaatggtta ttcctgtgac
    22981 aaaagaacca gctaacaatg caccaatgaa tgcccatggt ctggtcatcg tagcgactgc
    23041 aaacattccc gatgccggaa tacggctatc tgcgccccaa aacatgctta atccgccacc
    23101 tactgtccca ccgactcccg ttgctatgac attgcgtgcc aagtcgttca atacgatagg
    23161 gaaacatccc tctgtgatct gacagatccc cataggaaaa gcggtcttca atgtatcgat
    23221 ttcttgacga ttgagtatat ttttacgaat gattttccct aagaaataag cgatcgtcaa
    23281 gccgaatggg gttaccatcg aagctaaaat caatacagtg attggttcat agattccttc
    23341 tgaaaatcgt gccacacccc cttatccaga ataaatggta atccagaaac agccgtgtat
    23401 ctgcggtaat aagcctaacc aaacgaaaaa tatgagaagc tatttttgtt tggctgttct
    23461 tattacttga tgcagggcag ctttttcaca acctcttttt ttatttatgt ttcataatca
    23521 atatggtaag gatgcagctc cgtatggtag accgcctccg aatattcaat catttcccca
    23581 atttgcgtag acgatttccg tatccgtttg atgccgatct gtgcgtctgt tttcagaatt
    23641 ccttgttctt cttctgttaa aaatactgcc gagaaatcat cagaaaatga gtcaatagta
    23701 tatccttttt tcgtcagcaa acgatacaaa gattctttgc caaattcttc tctgctgaat
    23761 tgcatcattt cttgtggaag ataatgaaca aaataaatat aaggctgatt gttcaaataa
    23821 tataagcgct taaaacaaac agcttgctca ccgaaaaagg aaaacaaagg atcttccttc
    23881 tttatatgga ccaattgcaa atccagcgtt tctttccgga cttcatatcg ggagttctcc
    23941 aagatttgcg taaagcttgc cgctttggat aatcgattgt aaggacgatt gcttagaacc
    24001 gtcgtcccac gaccactttt ctttgcgaca tattcatctg atgccagcag ttcgattgcc
    24061 tttcggacag tgattttgct gacttgaaac agttcttcta attcactttc tgtcggcaac
    24121 atcgaaccga ctggatattt tcctgagaaa atatcttctt taattttatc tgcgacttcc
    24181 aagtatagta cacgtgtcct tttcataacg tcaccctttg ctttactatt actaaaagaa
    24241 tagcacataa aaaataaaaa aagaacacaa atcacatgtg agaagtgtgt tctttttcat
    24301 tcagctgctt taattacaag tttacgccat ttgaggcaat tacttcttta taccaatcaa
    24361 aagatttttt ctttgatcga ttcagtgtcc catttccttc atcgtctaga tccacataga
    24421 taaagccgta acgtttactc atttctcctg ttgatgcact gacaagatcg atacatcccc
    24481 aaggcgtata gcccatcaaa tctacaccgt ctgctactgc attagccatc gcttcgatat
    24541 gacgacgtaa ataatcgatc cgataatcat caataatcgt gccatcctct tccatatgat
    24601 caatggctcc aagaccgttt tcaacaatga ataatggttt ctggtaacga ttgtataatt
    24661 cattcaatgc gattctcagc ccttctggat cgatctgcca gccccattca cttgcctcaa
    24721 gaaacggatt ttttactcct cccagcaagt tcccttctga agttgctgct tgtggatcag
    24781 tctcattgat tgccgtagac atataataac taaatccgat gtagtccact ggatattctt
    24841 ttagcaactg gaagtctttt tccgtttgct ctaatttttc cggatcgact ccatattttt
    24901 tatgggtacg cgctgtgtat gcagggtatt ctccacgtac ttggacatcc gtgcagaaaa
    24961 aattgaattc ttggttttgg atcattgttg ctgcctgatt gactggattg gcatcgatgc
    25021 tgtaagttgt tgcatagata atcatgcagc cgatttggat atccgaacgc aattcgtggc
    25081 cgatttttac ggctttgcta cttgcaacga attgattatg ccatgcttgg aaaatatttt
    25141 ggtactctcc tgctccattg ctttttacta atccttgact taaagctgga aagtgaaagg
    25201 ccgaattgat ttcattgaaa gtcatccaat atttgacttt actgctgtat ctctctaata
    25261 ctgtctgagc aaatcgttca tagaagtcga tcagttttcg atttttccag ccaccgtatt
    25321 cttttgccaa atgaagaggc atttcgtaat gagaaatcgt gatcacaggt tcaatatcat
    25381 attttaagca ctcatcaatc agctgatcat aaaattctaa tcccgcttcg ttaggagtac
    25441 cttcgtctcc atttggaaaa attcgtgtcc aggcaatcga aaaacgataa catttgaatc
    25501 ccatttccgc aaacaaggca agatcttctt tgaaacgatc gtaatgatcg attcctcgat
    25561 gattcgggta aatatatttt tcttgatcaa tcgtccagtc aaattcttca ctgccgataa
    25621 tcgcaaagcg ctgcttacct ccaggcatag catctgcaac ggatagtcct tttccgtctc
    25681 gatcataagc cccttctaat tgatttggca gcagttgctc caccccataa aaatttttct
    25741 gaaaaacgtg attgcatcct gatttcctcc ttattttaca attgccagaa catcttcgtt
    25801 tgcaataatc tctttttggt ctaattcaag tatatcggca aatgcatcag tattcgtaat
    25861 gacaactgga gtgattacgc tatagccagc tgctctaatt gcttcgatgt caaaacgaat
    25921 caataaatct cctgctttga cgagatcccc ttgtttgact aagatctcaa agccattccc
    25981 atttaattct actgtatcca tcccgatatg aatcaaaatc tccgctccgt cagtcgttgt
    26041 gatccctaca gcatgtccag ttggaaaaat agtcgtgatc tctccgtctg caggtgcaac
    26101 tagttctcct acttctggat caatagcgat accttttccc atcacgccag aagaaaaaac
    26161 ttggtctggt acttcactca atggcagtac tttacccgtt aaaggacttt ttaaggtatg
    26221 ccgtgctgaa gtaatcggtt ctcctgcatt cgcatgtttg ttttctaatt gattgtcttg
    26281 tgtttgttcg tcaaatccta aaatcaatgt acctacgaat gcaagaacaa aagcaattcc
    26341 tgtagctatt actgctactg tcacatttga ctctacacca tccaccgtat taatgaaagt
    26401 cggaatcgtc aataagctga ccaaactgct ggagaaagct tttactccgg aaaatccgat
    26461 aatagctcca ccaatacctc cagaaataca agcggcaatg aatggtttct tcaaaggtaa
    26521 tgtcacacca tacacagttg gttcagtgat tccaaataac gaagtcactg tagaagaaat
    26581 ccctaatgct cgaagtttcg tatcttttgt tcgcaatgct acagctaaag cagccccacc
    26641 ttgtgccaag atggctggaa gaagcattgg catcaataca tcaaacccat attgttcgat
    26701 attcaaaaac atgatcggta cgaatccccg atgcatgccg aacatcacga atacttgcca
    26761 taaacttccc atgattagac ctgctacgat tggactaaat ccataaatcg agttgaagag
    26821 accgcctaaa gcattaccgg caaccgtccc gattggtcct aaagcaagaa atgttaatgg
    26881 aaccatcact actaaaacga caagcggaac taaaatcatt tgcagaaatt gcggaatcac
    26941 tttttttacg aatggctcta atttactttg aacccatacg gctagaatga ttggtatgac
    27001 agaagaagta taaccagatg gtgaaagaat cactgggatc ccaaagaaac tgatagcttc
    27061 tcccgcacca gcaactgctg tgatagctgg atacacaagt gccatagcaa ttgctactgc
    27121 taaaaactgg ttggctttga actttttagc tgctgtaaaa gcaagcatga caggtaagaa
    27181 ggtaaagaca ccatctgctt cagcaaacaa tatttgataa gtaccgctat cagctgtcag
    27241 ccagcccatc accgttgcca atgataagaa tcctttcaat accccggcgg ctgccatcgc
    27301 ccctagaaaa ggtgtaaata tactagagat aatatcgatc agacgattca aaatattttt
    27361 ggactcttct tttccatcct ccgaaactgc aagaccttcc tcatccacga tcgattgata
    27421 aacatctgct acattactgc caatcactac ttgatactga ccgccacttt gtacgacttg
    27481 gatcacgtcc gggtcttctt ttaacttttg cgtatctgct aaagattcat cttttaatct
    27541 gaatcgcaac cgcgtagcac aatgtactaa acttttgaca ttcttttgtc ctcctaccgc
    27601 ttcccataca cgacgaccaa ctgcttgatt atccattgtt ttttccttct ttctttttta
    27661 tttaaaaaat aaaaacccag attgaaaaaa acagtcacaa acattcgtga acctattttt
    27721 tcaatccagg ttttgcctgc catacagtaa caatccttat gattggatgc tttttttctc
    27781 acttactact ctttgaatat gaatcgttaa ataaatctgc tctgcgatcc ccatttcctg
    27841 tttttgcgtt tgtcgaagat actcattgat ccgttcgaca caatgaaacg cttttggata
    27901 ctttccttga actaatgcgt agagaaattc atcttgctcg tcagatcctt cccctttgag
    27961 ataacgctgg gcaaaaaact gcagatgagt aatgatacgc tgataattca aagattcttc
    28021 atcgaacacg atgccgaaga atttactgat gatactcagt atatctctta ccattgtggt
    28081 agcggtcatc gtcacttgca tgtcttcgtt tccttgttgg ctattcacaa aatgaaaggc
    28141 aataaaaccg gcttcatcct cagaaagttc cacacccgtc ttttgtttca cgaacaaaag
    28201 agcctctttc gctgcttgat attctttcgg ataaaatttt tttgtttcaa ataataatgg
    28261 attaggcagt tggatatttt cttttgctcg tgtgatcgca taatgaagat gatcggtcaa
    28321 tgtcagataa atattaggcg aaaattcaac ttttagttta tcttcagcca gttcgactac
    28381 ttgtttgacc cattcgatct cctctgccgg aatatcagcc atcagctgct ggaattggtt
    28441 tttagcaaaa gaatctctta aaacaaattc tttttggacc gcttctggcg aaatctcgtc
    28501 cccaatcttt ttttgaaaag ctagtccttt tcccatacaa acgacttctt ccccgaatcc
    28561 atttttcgaa atgacgacat tgttattcaa aattttatca attttcattt tgcttcgccc
    28621 tctcaagaaa aataaaaaaa cctagaattc gcctactgtt tcttagctga attctggttt
    28681 tgcctgccgt acagtaacaa tccatttctt attcaaaatc atcataccat cgaattttta
    28741 tttatgcaag ccttttcata taaaaaataa aaaaactgga acacaagtct atctagaaaa
    28801 caaccaaatc agagatgcac ctctttgcgt ttctcttttt ttcagcaaat tggtttttcc
    28861 ttatcgattc atatcctttg gctgttttta atctgtaacg acttatgtcc cagtatccta
    28921 aaatgttttt gtctctagat tgaaagcaga tctcatatca tctgtcgcat cttttatcga
    28981 tgtaacactt tccagttctc cttgtacaac tagtcgcgtg acggcgtcca tgtcgccaca
    29041 agtaattagt gtgacaattt cttttccctc gacttcattc agtaattggg tatctgtagg
    29101 agcaactttt tctttaaaaa atgttttgta tgtatacaca ttttctaaat ctgtcaaata
    29161 gatcttggca cccatctcta aattttctag cggtgtaaac agcaattcag ggttcgttgc
    29221 acgatggctt gctaaggcat aatttccttt tcccatttct tgatctggag ataatgttcc
    29281 tgctccgtaa agtagtgacg tattatccaa gcctttgaaa accggtaaat tgattgaaac
    29341 tgaagggact gcgatactag caatcactgg tagatctgtt cctctcattt gcgacctcat
    29401 gaccccttct gtcgtcattg gttctaccgc atcaaagtca aaactgacat ctttatcgtt
    29461 attctttttc agatcttctt ttgttacatt cgctatagca tatttatctc ctgtctctct
    29521 gacaaaataa tccttgatct gttcattgaa aatcaaagct agtcctacta ataacaagag
    29581 gaacaagagg atattgacta gccatctttt tgcttttcct ttttttttca ttttctctcc
    29641 ctccagactc attttactgt ttttcttttg ctctttccaa cagccgaatg acacatttct
    29701 acaactctgt gtattttttt cgtacacctg cgcttttttc tacaggatat ttcaaattat
    29761 ttttgatcaa tttttcttca atgatctcat ctagatccag gtgaaggttg tctgccatca
    29821 tgtatgaata gatcaataca tctgccagtt cttctttgat tcgttctaat tgtgtttctt
    29881 ttacttcttc cggcgttttc cattgaaaaa gttctaacaa ttcagatgct tccaaagaaa
    29941 tagagatagc caaatctttt tcgttatgaa attgtcgcca attacgttca tctctaaact
    30001 ggttgacttt ttccatacta ttcatttgct tgccttcttc caatcacttc ttacttgatt
    30061 aatcatacca tttttttaca taaaaaatag atgatgcttt taaaaaaact cctgttttct
    30121 tttatttaaa ataaatgtca tagaaaatca ataatttagg tagccaccat gtgtatgcag
    30181 tagcgtgtct tcttcccttg aggctttttg tttttcgata cgttcttctt cccaaacaga
    30241 caacgctact agaatctctc ttaatgattt tccttcttcg gtgagcgagt actctacttt
    30301 aggcgggact tggttatagg cttttcgatg gatactttca tcttgttcta actctcttaa
    30361 ctgctgggtc agcatttttt ttgtgatcgt agggatttgc ttgcttctcc acgcctaaaa
    30421 taagcgatat tgctcttccc tttatctggt gtaagcaaag cagttagcat tttgacgatc
    30481 gtgggttccg cttttcactt cttatctact tagtatcatg aagtatactt tcgtactaat
    30541 actaagtagc ggaagtgtca actactttgt ttaactacat agcaaaagaa acagaagggt
    30601 gacaacggtc atcacgcttc tgtttctttt gaatatgctg tgttgttgtc tctatctccg
    30661 tgtagacttt cctcaaactt atcctttgac agtaagtcag taggctgatt tgctctttct
    30721 agatgccaga tacatttcta caaaaaaata gtgattctgt tttgaaaatt cgatttttag
    30781 ttctgcattt tcgtaaacat ccaacatttt tttcgcactg tatagaccta acccgccatt
    30841 ttttttagag gtttttcctc ttttgaccat ctccgatagc gtcaaattcg ttggatttgc
    30901 cttgttcgct atactaaaac gtgatccctc tttttcttcc agcagattga tcgtaatcgg
    30961 actctgatcg gctacacgat gatcgaatgc gttagaaata aaaatagata aacagcgaat
    31021 aaaaacaacc agatcaatcg ggattgtttt cacttcgaca ggaataacta cttggatcgg
    31081 gattttctcc gattctgctt tttctagaaa ttttaaaaag acacccctga cagctgggga
    31141 tacaatattt gttagctgtt gtatttgcgg tttttcaaaa atagctgcag aatgatcttc
    31201 taatatttct aaaaattcca acgtttcttt tttattttcc tgaagagtaa tttttaatga
    31261 aaacagtaga cttttataat catgtttgaa ttcctctaga tgatggtaat attcttcatt
    31321 tatttgatac agttgaagcg attcctgatg ttgtctttct tttacttgga aactgatcaa
    31381 cataatggaa aaaaacaaag aaaaaaaact gatgactatc gacaaaagag atagaagcag
    31441 ataattagat gagcggaagt gaacacttcc caataccaag taaatcacgc cccagcttaa
    31501 taaaatacaa atacctccaa aatagtgttc agttggataa gtaaaaagca agttgagaaa
    31561 tctcgtctgt ttgtctatac tttttactaa tagatagaaa ataaatgcga taagtagtgc
    31621 aaaaaaactt gctgttccaa gatgtctctt tcctaaataa tccatcagat caaaaaccag
    31681 aaaaaagctg agtccagtga tagcaaaaaa aagattactg atcaaacagc tgataaacag
    31741 gtttttcgta gtaaaataaa aaagaaacat caataataag ccaactaggt aaggaaagct
    31801 cactttaggg ataggaagta aaagtgtcac caccatcaaa gctatacaac caaagctgtt
    31861 ttttactagc agttgtttcg aaaagaataa atagatacct aaatctaaaa tcaaagtaaa
    31921 ggtcaaataa agtgtcactg ttgcgaccat cttattcctc cattgttttg tttcaaaatg
    31981 tacacatatt tttttaatag gagcctgcat atgaaaaagt attttatcat agaagataac
    32041 cagtttcaca gcgaatggat catcagagaa ttggtcaatc atcatatcga gaatatcgtg
    32101 cagctcactt ctatctctca aatcaatcag ttgatccagt tgggcactat tgataatcac
    32161 gatgttttct ttatcgatat cgacttgaag caatcactca caggtattga tgtcgccgaa
    32221 caaatacgta aaaaaaatgc acatagtaat attgtttttt tcaccgctta tacgaatttt
    32281 gcagtagaga tcatcaatcg gaaaatcctt ccgattggat atatcagaaa agacaggaat
    32341 ttatctgaac aaattcgtca agtgctagaa cagatcaatt ggaaagaaca gcagatcgta
    32401 aatgtcgata aaatctttat tgatcaaaaa ggtggaagtc tcgtcctgta tcctcgggaa
    32461 attttatata ttgaaagtat caaaggtttg aaaaaccaag ttatggtcaa aacaactaca
    32521 gaagataaat tagtgaatac ttctttaaaa caattgaaga cagagttaga aaagtatgct
    32581 tactttttac ttttaaaatc ttactgtatc aacttatcgc aattgcagaa aattgattct
    32641 gctcattatg tgttagaatt tttcaacggt gactccttac tcgttggtcg aaagatcttt
    32701 gaaaagacaa aggaacgttt ccatgacttc caaaaaactg cttcgagttg atgaataaga
    32761 gctgagacgt aagcatagca gatttcaggg agtgggcctt gaatcggctc cactccctga
    32821 aatcgttgat atgattttac gcttcctctt ctgtaggagc atctgttaac gaccatgtga
    32881 atgttgaaac gtagttgtct acagcataat tggatacagg aacaaataat tccactgatt
    32941 gtctcccttg ttccacattc tctcctaatg aataaatcca tgtgccaatc ccttcatttg
    33001 ctcctgcagt tactagatct actgcctgat ctgctgggat ttcgattcct tcagcgccag
    33061 ttccagaaac ggtagatggc attccactgc tattagttgc gctatttcga atcacggcgt
    33121 ttttgaattt cacctttgaa ccagttagtt tattcgtatc agatgttgta ccaataaatt
    33181 ctgttcgttt gactgataat ttccaaccgg cataatttcc gcttttatcc gttacttgta
    33241 cgaaattctg tacttgtgtt tgtgctccag tactgtccgt tccgctttgt aatgcagcat
    33301 atactgtttt tgctgtacct aacttgattg ttccaaaatc aagatctgga gcttggtcaa
    33361 tcgtcagtaa tccggatgtt ccctgatttg gttcgtcagg tgtaaaagga gtatcaggat
    33421 catttggatc aacaggagga actggaacaa cgtcttcagg atttgcagca tctggtttgg
    33481 tcacagaaat cgttccttct gtagtggctg agctcttatt ctcagcaaaa acactttgtg
    33541 tacataataa tagacttcca gctagaacgg tacttaatgc aattgttttt ttcatttctt
    33601 tttccttctt tcttttaaaa taaataatcc agcaaataag ccaataagaa tgactccaac
    33661 aagtatcagc catcgccagt caaatgtttc tggctgattt tttaaatcaa tatccttttc
    33721 attcaattgg tccgctgcat catcagttat ttcaaaatcg gtatggagat tccagcgttt
    33781 ttctttccaa gcacccttca caaccgcttg ataatgtccc ggagctaatt tttttccttc
    33841 taatgggaca agtaaatcaa aatttgtatt gggtgctaca tctatttttt ctttttttat
    33901 tgaaaggtaa ggctcttttg tttctttttt gtagatttca accgatgtag tcaagccgaa
    33961 catataagaa gcatttggat tttgaagatt cgccttaatc acatttcttc catcgctttg
    34021 atccgcataa gcttttttta atttcaaatc tggggaagac tctttttcgc tatttcaaat
    34081 caagattgct ttcacttgag aaaactgatt gacgacacca actgtacttt tgttagaaac
    34141 gttctctttt ttttcttgaa acgtgatccc tcctgcgacc acaccagcca gctctttttc
    34201 tggcgctttc agtgaaagtg tctcagtgac cgcactttga ggaggaacag ttatttctcc
    34261 cactgttttt gctaagtttt tcatatcata acttaatgtt tcatcaaatt tactttttga
    34321 ttctccatat tcgatcacac cgtttgaatt cgtcgtagcg gagtggatca atgtctcaac
    34381 tgttactgat ttatctgttc ggttaaagac cgtgtagggg atttcctgtg ttttcccagg
    34441 ctccatcctt acatttatat atgttttgtt cttatcaatc tgattagccg gtaattcaaa
    34501 atctaccgca aaattgaatt cggaggcatg gacaggtgtc tgccaaagga tgaatccaag
    34561 gagaaggatc ataagtaaag agcagttgtt tcgatgactt ttattccata ttcgattcat
    34621 caatcatcct ccttgatctt tattctgtcg gcgcatctgc taatgtccat gttatctctg
    34681 ctgagtaatt accagctatc tgattttctt tggttaagac aagtttaaac gcccgaccgt
    34741 tatttaaata ttctgaataa tctgcttgca cattcgttga attttgtcct ctgacaattt
    34801 ccagttcttc ttcattcact aattgatact cctctccttt cttgaaatat aggacatcct
    34861 gcatagaaaa tcctgcatca tttgtgaaag gctgagataa ttttatcgtt aagatccacg
    34921 gatttttcgt ttctcgctga tcataaatcc ataatttttc ctgtatatcc atttggccaa
    34981 atgcttcttg tgtcaaacca gttgctttga tctgaccaaa atcgagtttg gaaggggcaa
    35041 gaaatgagag accaccttct actacgatct tcgtagcagg tgcaaaaggc ggggcatcat
    35101 gataaacaac tggcgtatca cttggattgt gattcccaat ttcatcgttg gtacttggct
    35161 tgttctgcac acccttagca gtagcttctc ctgcttggtc gtttaataca atttctaata
    35221 catctcctgc aaaaagttga ttttctggga tagaaagaac aaattcccct gtatcatcaa
    35281 tagttgtttc tgacaaaaca gcttgatttt ttttcaatga aaccaaggct cctgcttcac
    35341 ctgtacctgt gatcgtgcga gaagctgcat tgatcaatgt ggtacttaaa ttagcaggtt
    35401 caggaggaat cacatcgact gtcgttttgg cagagtccag ttctgtcact tcttggccac
    35461 ctcttacttt tttagcagca acaacttgca cttgatcctc cgtttttaat ctctcatcat
    35521 tgggtacttg aacttcgaac aagccgcctt gttcgccttc tccccatggg ttatatcctg
    35581 ccccttcatt ttcatctgtt tgtccttgcg tgagagcagt aatttctaca gatgatttcg
    35641 tcgtatctgc gtaagtaata cgtaaagtca cttctacttc gcctgtccaa gcatctcttg
    35701 gctttgcctc gacaccttcg ggaacaacga catgcccata gattttttta tctgcattcg
    35761 ttggcactct taaggattca atcatcggga gctgattatt ggcactgatt cgattataat
    35821 ttttcattcc ggcacctgtc tgagcaaatt catttgttaa acctgcacta gggcttgata
    35881 cgaaattgcc taagtcggtc ccattgatcg ataagtctgt catatcagaa aaataatccg
    35941 ggtctttttc aaagttatcg aatgttgcgc ctgtttttcg ccataaagcg atttctgaat
    36001 gggcgatctt taaagaagaa tctccatttc cttggaatac ccagccgcct atgccattgg
    36061 tctcgcttcc aatccgtttg ttttggaaat caaaataggt aggagattct aagtccacat
    36121 tcaacacatc cccactgatg atgccggtct tatcttcccc tgttttcccg accatgacta
    36181 aataagtttt ttcacctgcc gtaaaattta aatcaatatc tccagttgta tctacagttg
    36241 ctccggaatc tgcgcgtata tcgatatttg atttttctcc agtgactgta aaatcagctg
    36301 aacctggact ttctagaaaa ccagaagcgt cataaaattg gatcgcttga tttccttgat
    36361 tgagtctgtt cacgtaagat ttgccatcac ctgtatttct taccctcaat gagcctccat
    36421 tggttactgt cagcttttgc gtaccattct caaaacgaac tgcgctggaa cgcccactct
    36481 gcttatcgat gtcgataact gatccggctc cttctacgat caaagaaagg ccttttccta
    36541 caaaacgcat cccattgttc ccgggttgat cgtcatctcc agccattttg accgagattt
    36601 gtccaccact tgatacatgg atttgactag aatctccttg caagcgaaat ccgcccgtag
    36661 ttgctgtatt gatggtgatt ttcccaccat tcgatacagt gactttcgat cctgaacctc
    36721 tcatttggat gattccgcga ttgttgtcat tggtaatatt gatctttgta tttaccgaaa
    36781 attcgcttcc cgaaccactg acttcaaaca tgacattacc aggtagcgta tcgactgcct
    36841 ctccttcatc gctaacgcca tctgcccgat cataatcaag agagaccttt gaaccgctta
    36901 agatatggat tcctggcgct gtttttccac taccagtctc atctcctgaa tctagaaaac
    36961 cgtagaattt ccctagatac ggtccataaa gttgactacc attgatcaca ttcaattgat
    37021 agcttctaca aaaataggct ttacccggag cggcattcag tccttccttc actggttgga
    37081 catccaacac accggaaaca ccatctaatg tgacagttgc acgaggagcg taaatcagtc
    37141 ctaatttgga cccatcatac aaatcaagtg tcccggtaaa ttttatttct cccgtaaaat
    37201 gatatctgct tccccaaaaa tcggtcatat aagcaatgat aatggcattt ccatcgatat
    37261 ttggtgtcaa tccacctgga tgtgcagctg ttcctttaaa attctggaac tcgatcgact
    37321 gaggtatctc attgatataa atatggtgtg tgccgaaatt gtgagcgaat ccaccaaaat
    37381 caataacgat atctcgtttg atctctaatt ccctgtctaa agtcaaaggc tgaatcagtc
    37441 ggatcttagt tactgattcg tctgcgagaa gctgcgccag ttcttctgtt gtggcagctt
    37501 cgctgtaccc atccgctgaa acagctcttg ccagtgccgc ttcactgcta tcagacgttt
    37561 gttcagctac ttctgaggtc tcacttgtct tattttcttc ttgcctttta ttagtagaag
    37621 cctcactttg agattctgta gtttcagaag taatcgattc ttctgaaaaa gtagtcttgt
    37681 caaaactttg attctctgat tgcgttgatt cgtttgtttt ctctacatcg gcaaaaacgg
    37741 atgtcaccat caagagaggg aagacaaaca aaatcgacgc aatcagcttt tttacacatt
    37801 gattcattct ccaccatcct ctctgacgtt ttgacaattt tattttatcg ttttaatcta
    37861 tttccaattg tactttcata tttaaaatat gtatttttat ggaaaaattt ttctattagg
    37921 tgtaaatttc ttttttgtac aaaaaaaagg ctgtgacaaa agtcttgtca cagtccctta
    37981 tcccgaacaa gcggtgttct cccgccaact cctcggtcac aacctccatt ttaacaataa
    38041 acctgtaaat gtgagggcaa tactttgatt gtcacaggta atttatcccc ttcatctcca
    38101 tcaacattcg tcgtcaattc ttcctcatca gctaatgaca caactaattg ctcacaagta
    38161 aggtaggcta gatttttcgt agattcatct actcctttta ataggtctgg caatgtttta
    38221 agcgtatcga tcaaagaaga atcttttaaa taaaccaaat ggagcttacc atcatccact
    38281 tttgcatttg gaagtaatgt ttcaaaaccg ccgactgagt tagttagtcc tatcagcaag
    38341 gtactgctct caatttcttc cttctttcca tcaacttcta cgtgaaacga gtaggattgt
    38401 gtggaagcta actgtttgat tcctgacatg aaataagcta gttttccaaa tttcgtcttc
    38461 ttctccgcat ctacatcatt gattgattca gggatagagc caattgcgac gacattcata
    38521 aaatagtcgt catttatttt tccaatatcc aatggcttga ctgattcaat cgaaaaatga
    38581 ttgatcgctt cctcaggttc tagcggtatc cctaatgctc gagccaagtc attcaccgta
    38641 cccaatggga aaaatccgaa atttggccga tgttcttgtt cagcgattcc actgattcct
    38701 tcgttgactg ttccatctcc gcccatgaca aacacactat gatacccttc cgtagccgct
    38761 tcgcgagtaa agttttttgc atcccctgct ttttttgtat gcaagacgac cacttcatca
    38821 aacaccgatt ccaattttgc tatcgctaat tgctcaaatt cctttgcttg ttcaccgcct
    38881 gaactggggt ttaccactaa taaaactttt ttcatcactt tctttccctt ctgattgatt
    38941 tctttaatat aacgatagca taaagaaaaa aattaggctg tgatgaagta ctgttcatga
    39001 aacattcatt tattttttga caagataaaa catccatact atgaaactga ggaaaattaa
    39061 aacaatgata taggaaataa aagaaaatga taggcattgt atacctaata aagtaaaaat
    39121 cttaggtggc agataaaaac acaaaaatag caagagaatg taatagcgtt tcttccactt
    39181 tttgttatca gaccttatag cgatcagagg aataccaaat gcataactga aaaactcttg
    39241 atgtactgtt aatatgtcac ggccttgaat aataaatgcg acaatccccg ctataattag
    39301 tgtgcctaac accaaaacgg aaaattgatg aatatatttt atatacaaat gatccattgt
    39361 acttcctgcc tttttatttg tgagaaaaac aaatccgtat acgtgaataa cttctagaaa
    39421 taaaagaaat ttgcaaaacc actccattta tctacttcta cgtgcatttt ttgatattgt
    39481 tttctttatt taagattata cagaaaaata tctagggtcg ctggattcta agtaaaaaaa
    39541 atttttaaaa taaaaagaac taaatcaagt agtaattaaa ctactgttga tttagttcta
    39601 ataaaaagat ttagatgaac agttttattg atttaaatag ttcatatttt ttatttatac
    39661 cgcttattta ctacttgctg tactagaatc tgaagtgaat gtactcaaaa cattttcaaa
    39721 gtctttgtct ttgattttta cattagcgtc tttcaatact tcgccaatta ctttattctg
    39781 gaaggtactg tcacttaatt ttgtgtctgt tgcaatttct ttcaattctt ttttgtattt
    39841 atccatgtcg ttgcctttgt tttggttttt caccatttta accacataat attccgtagt
    39901 ataagttgaa gcattggtag aagtgatcac atcagaaact tgaccatctt tcaacttaaa
    39961 ggctgcttct ttgacttctg ccggaaccgt tgtagtagtg gaatcaaatt tcacttttcc
    40021 gccatcttct tttgtcgtag tatcagttga cttgtctttg gctaatttag agaaatcgtc
    40081 tcccttatca gcagctttct tcacatcttt tgcatcatct tcacttgcta cttggataat
    40141 ttgagcttcc acttctggat ggaaagattc ccaagccgtt tttaaatctt catcggtcaa
    40201 tttgatatgt ttctttagac cttcttgaaa ggctagactt tgtttgatac tgtctttgaa
    40261 tgtttgttct gtgtaacctg cagatttcaa ttgtgaatca aatgaatcac ccaattgttt
    40321 ctttgtttga tcatattgag aatcgatttc tttggttgat acgtcatctc catatttatc
    40381 ttcaaatact tgataaacga tcaactgttg cacagcggaa tctgctcctt gcattttttt
    40441 caagctatcc attacttctt gttcgctgat tgtaccgcct ttataactaa cggctgtttt
    40501 ttcaccggca ttcgtacagc ctgacaataa gccagtgcct agtacgatgg caagtgctaa
    40561 aaatcttttt ttcatttatc aattctccta cttttcgttt tttgcgaatt gctaaaaaca
    40621 aaggtgttca agatgaaaat catcaagaac acctttacta atctgctcga tacgtcctga
    40681 atagatataa ggctacacaa tccagaaagt tcgctcagca gaataaatgt caagtagcac
    40741 atcacacctt aatatactac ttcttttgtt accgtttttc aagtctatct tgcgttccgt
    40801 atttaacgta actaattctt agaacaaatg ttatcgattt tgtttttctt cttccgctgc
    40861 tgggatattc aaatcagaca cttttatgtt cgattttcca gacatttttt tgttgttttt
    40921 attatgatat atttttata
//
LOCUS       DACKAC010000018        39958 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.018, whole genome shotgun sequence.
ACCESSION   DACKAC010000018 DACKAC010000000
VERSION     DACKAC010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39958
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.018"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            208..1014
                     /gene="proB"
                     /locus_tag="I0B46_001010"
     CDS             208..1014
                     /gene="proB"
                     /locus_tag="I0B46_001010"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAR2083324.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            1011..2258
                     /locus_tag="I0B46_001011"
     CDS             1011..2258
                     /locus_tag="I0B46_001011"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAR2083325.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            2334..2678
                     /locus_tag="I0B46_001012"
     CDS             2334..2678
                     /locus_tag="I0B46_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083326.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(2795..3121)
                     /locus_tag="I0B46_001013"
     CDS             complement(2795..3121)
                     /locus_tag="I0B46_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083327.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(3494..4696)
                     /locus_tag="I0B46_001014"
     CDS             complement(3494..4696)
                     /locus_tag="I0B46_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAR2083328.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            4813..5328
                     /locus_tag="I0B46_001015"
     CDS             4813..5328
                     /locus_tag="I0B46_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAR2083329.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(5527..5973)
                     /locus_tag="I0B46_001016"
     CDS             complement(5527..5973)
                     /locus_tag="I0B46_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAR2083330.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(6020..7498)
                     /gene="msr"
                     /locus_tag="I0B46_001017"
     CDS             complement(6020..7498)
                     /gene="msr"
                     /locus_tag="I0B46_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAR2083331.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(7852..8481)
                     /locus_tag="I0B46_001018"
     CDS             complement(7852..8481)
                     /locus_tag="I0B46_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAR2083332.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(8465..8929)
                     /locus_tag="I0B46_001019"
     CDS             complement(8465..8929)
                     /locus_tag="I0B46_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAR2083333.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(9416..10684)
                     /locus_tag="I0B46_001020"
     CDS             complement(9416..10684)
                     /locus_tag="I0B46_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAR2083334.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(10915..11652)
                     /locus_tag="I0B46_001021"
     CDS             complement(10915..11652)
                     /locus_tag="I0B46_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAR2083335.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(11719..12288)
                     /locus_tag="I0B46_001022"
     CDS             complement(11719..12288)
                     /locus_tag="I0B46_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAR2083336.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12416..13288
                     /locus_tag="I0B46_001023"
     CDS             12416..13288
                     /locus_tag="I0B46_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAR2083337.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            13630..14502
                     /locus_tag="I0B46_001024"
     CDS             13630..14502
                     /locus_tag="I0B46_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAR2083338.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            14499..16457
                     /locus_tag="I0B46_001025"
     CDS             14499..16457
                     /locus_tag="I0B46_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAR2083339.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(16708..17559)
                     /locus_tag="I0B46_001026"
     CDS             complement(16708..17559)
                     /locus_tag="I0B46_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAR2083340.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISILAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            17682..18149
                     /locus_tag="I0B46_001027"
     CDS             17682..18149
                     /locus_tag="I0B46_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAR2083341.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(18412..18894)
                     /locus_tag="I0B46_001028"
     CDS             complement(18412..18894)
                     /locus_tag="I0B46_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAR2083342.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(18994..19857)
                     /locus_tag="I0B46_001029"
     CDS             complement(18994..19857)
                     /locus_tag="I0B46_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAR2083343.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            20455..21303
                     /locus_tag="I0B46_001030"
     CDS             20455..21303
                     /locus_tag="I0B46_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083344.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(21474..21815)
                     /locus_tag="I0B46_001031"
     CDS             complement(21474..21815)
                     /locus_tag="I0B46_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HAR2083345.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            21901..22608
                     /locus_tag="I0B46_001032"
     CDS             21901..22608
                     /locus_tag="I0B46_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAR2083346.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            22595..22918
                     /locus_tag="I0B46_001033"
     CDS             22595..22918
                     /locus_tag="I0B46_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAR2083347.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(23007..24044)
                     /locus_tag="I0B46_001034"
     CDS             complement(23007..24044)
                     /locus_tag="I0B46_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAR2083348.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(24491..25474)
                     /locus_tag="I0B46_001035"
     CDS             complement(24491..25474)
                     /locus_tag="I0B46_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAR2083349.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            complement(25890..26660)
                     /locus_tag="I0B46_001036"
     CDS             complement(25890..26660)
                     /locus_tag="I0B46_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAR2083350.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(26653..27588)
                     /locus_tag="I0B46_001037"
     CDS             complement(26653..27588)
                     /locus_tag="I0B46_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAR2083351.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(28182..29831)
                     /locus_tag="I0B46_001038"
     CDS             complement(28182..29831)
                     /locus_tag="I0B46_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAR2083352.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(30026..30922)
                     /locus_tag="I0B46_001039"
     CDS             complement(30026..30922)
                     /locus_tag="I0B46_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAR2083353.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(30915..31595)
                     /locus_tag="I0B46_001040"
     CDS             complement(30915..31595)
                     /locus_tag="I0B46_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAR2083354.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(31596..32378)
                     /locus_tag="I0B46_001041"
     CDS             complement(31596..32378)
                     /locus_tag="I0B46_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAR2083355.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(32371..33288)
                     /locus_tag="I0B46_001042"
     CDS             complement(32371..33288)
                     /locus_tag="I0B46_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAR2083356.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(33291..33575)
                     /locus_tag="I0B46_001043"
     CDS             complement(33291..33575)
                     /locus_tag="I0B46_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAR2083357.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            34363..37590
                     /gene="fss3"
                     /locus_tag="I0B46_001044"
     CDS             34363..37590
                     /gene="fss3"
                     /locus_tag="I0B46_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAR2083358.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVALVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            37703..38017
                     /locus_tag="I0B46_001045"
     CDS             37703..38017
                     /locus_tag="I0B46_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAR2083359.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            38030..38404
                     /locus_tag="I0B46_001046"
     CDS             38030..38404
                     /locus_tag="I0B46_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAR2083360.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            38414..38749
                     /locus_tag="I0B46_001047"
     CDS             38414..38749
                     /locus_tag="I0B46_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAR2083361.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            38832..>39958
                     /locus_tag="I0B46_001048"
     CDS             38832..>39958
                     /locus_tag="I0B46_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAR2083362.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRD"
BASE COUNT        12155 a         8142 c         7384 g        12277 t
ORIGIN      
        1 tactcctatt ctaacatatc cgaggctgta aaagactttc tttacaaatt cttcaagaaa
       61 ttctgctatc ttaaagtaaa ccattaattc gttctttttc caaacgactg caagttgtac
      121 aaaaaaacta ttttttacat gactttactc gtcaaaacaa gtagaataga acgaaattaa
      181 tgagtggata tttgattgag gtgtcacatg cgtaaagaaa ttaaaaatgc aaaacgaatt
      241 attgtcaaag taggaacaag tacattgatt tatcccaatg gaaatatcaa tcttgcggca
      301 atcgatcagt tagcttttgt cctttctgat cttagaaatc ggggaaagga agtgatctta
      361 gtctcatcag gagctgtagg cgttggtatg cataaattac atttgtcaga acgtccgact
      421 ttgattcctg aacagcaagc tgcggcggct gttggtcaag ccgaactgat gaatatctat
      481 aatcaaagat tcagtgtata taatcaacag acggcacaga tcctcgtgac aagagacgtg
      541 gtagattatc ctgaaagtcg taaaaatgtg aggaatacat tggaacagct tttgattatg
      601 gacacgatcc ctatcgtgaa cgaaaacgat actgtagcag tggatgaatt agatcatctg
      661 acaaaatttg gcgataatga tcagctgtca gccatcatgg caaaacttgt cgcggcagac
      721 cttttgatcg tcctttctga tatcgacggg ttttattcag ataatccgac tacaaatccc
      781 gaggctcaga tgtatcgcta tatcgataaa atcgatgaaa agattctggc acaagcaggt
      841 ggtgctggta gcacatttgg aacaggcggt atgcagagta aattaaaagc cgcttctagg
      901 atatttgaag caaacagcgc aatgatttta gcaaatggaa aaaatccggc aattattttt
      961 gatattcttg caggaaaaga agtaggtact ttatttaagg aggacttgaa atgactgact
     1021 tgatgcaatt aggacaacag gcaaaagata gcgcgcaaca actagcactt atgcctacaa
     1081 accgcaaaaa tgagctactt gttcaaatgg cacaaaccat caaaaagaat caacaagcga
     1141 tcattgaagc aaatcataaa gacttagaaa aagcagtcga aaacaatatc agtgaaacga
     1201 tgcaagaccg cttgcagctg acacctgagc gaatcaatgc catggctaca gagatcgaaa
     1261 aaattgcttc gttagacgat cctattggaa aagtagatga gatgtggacg aacaccgacg
     1321 gattgagaat cggcaaaaag cgcgtgccac taggcgtgac cggcatcatt tacgaatctc
     1381 gtccaaatgt cacgacagat gcagctagtc tagcattcaa atcagggaat gccgttatcc
     1441 ttcgcggtgg caaagaagca ttcttctcga atcaattatt agtccaatta ttgcagcaag
     1501 tactggtcag tgctggcgaa tctccttatg ccatccagtt tgtcgatgat acttctcatg
     1561 aaactgcagg acagttgatg cgattgacag aatacttaga tgtgctgatt cctcgaggcg
     1621 gagcaaaatt aatccaacga gtcaaagaac aggcaacggt acctatcatt gaaacaggta
     1681 caggaaataa tcacgtatat atcgataaag aagcacagtt atctatggca gtgaacatca
     1741 tcgtcaatgc aaaagcttcc cgtccatctg tctgtaacgc ggcagaaaca ttgctgatcc
     1801 attctgagat cgcacctttt ttcttgccag ctattgaaaa agaactggta gaacacgggg
     1861 tctctttacg tgcggatgct cgtgcgttag aatatctaga gacggctgct ttagcagaag
     1921 acacagattg ggatactgaa tatctagatt atatccttgc tgtcaaagtg gtcgattctt
     1981 tggatgaagc aattgcacat atcaatcggc ataatactaa acattcagaa acgatcgtga
     2041 cagatagtta tgcggctagt caacgtttct taaatgaagt ggacgcggcg gctgtctatg
     2101 tcaatgcctc cactcgtttt acagacggat cggtatttgg ttttggtgca gagattggta
     2161 tctctactca aaagttgcat gcccgtggtc caatggggtt aaatgaatta acttccacaa
     2221 aatatatcat ttatggcgat ggacaaatcc gcgaataatg tttcgtgtga aacattattc
     2281 ccgccttttg ccttttttct gtaaaggttc tatccaacta gaaaggtgtc aatatgtttg
     2341 aaattttaat gatttttttt atttacctta tttctctgaa tatcgctgct tttctgggag
     2401 tcggtatttt atcccttttc tttcaattca aaaaacgaag catagggagt caacgggaaa
     2461 aatggtcgca gtattttgac aaaatcgggc aaaaagggct ggttacacgc ttgcacatta
     2521 gttacatggt tgcattatgc ttattagcga caatcaatta ctattctttt ttcgatcatt
     2581 caatcgctta tacgattacg ctactgattg ctgggatttt tcacttatct tataaatatc
     2641 agctgaataa aaaccacttg aatcggactt tccgctaaaa aatatccgta cgataagtag
     2701 agtgccttga agatcggcaa aaaactgccg gactttttag aggctgtgac aaagattttg
     2761 tcacagcctc tttttccata taaacggcgt tcgcctacca gctgcctggt aaatctgttt
     2821 cctcttcgtt aagaagcgaa aatccgtcaa atgtttcgcc atcataataa cgaagcagct
     2881 catccatgga caagttcatc gttttttctt tttcttcttc cgctgattca gccagtagct
     2941 gttcccgata cacctgatac ataccagcta ttttttgctg catcgctttt ctagtttcac
     3001 cttccgcatt gattattggc aattctttga tatagccacg ataaatcaac cgatcatctg
     3061 aaaattcaat gggtaattca acgagtgtca acccgacttc ctgaaaatat tcttccagca
     3121 tctattatcc ccccattctt tttcttaaat gaattttagc ttacctactg aaaattttcc
     3181 agtttcatta ttttgagaaa aaaagggaat gtgacaaaaa tcgtgtcaca tccccttttt
     3241 ccgaataaat ggtgttcttc tgccaactcc tcaatcataa gtcacaatat tcaacaaaca
     3301 tgagaaactg ttttttgaaa ttgttccttg tgattcagag ttaaacgaca gtctcacaac
     3361 ctcttggtaa acggcgttca aaagcttgca tctgcggtaa taagcccaac caaacgaaaa
     3421 atatgagaag ctatttttgt ttggctgttc ttatgacttg atgcaggaca gctttttcac
     3481 aacctctgat ttcttattct tcttcatttg tagcaaaata attacggtga taaatgatac
     3541 aacatacact gataagaaca atggcacctg caaaataaaa cgcatagctt tcaccaatat
     3601 gttcaccaat cacacctgca ataaatggtg ctaaagctcc accgaaaaag cggacaaaac
     3661 tatagctgga cgaagcaacg tttcgttgca gacctggtat ttccatagca atcgtcgtca
     3721 acagtgtatt gataattcct tggaaaaatc ctgctaacac aatgctgatc ggtacaagga
     3781 ctggcataga agttccagct cctaataaca gcaaacaaat aatgaataag acaaatgctg
     3841 ctaaaattgt ccgataagtc gtaaacttcc gctcaagagc cggtgcaacg aaaatcgaag
     3901 caatagccag aagcacaccc cagccaaaaa atacaaaacc gacttctaat tcagaatagc
     3961 caatcaataa aaatggcgca tacgcaagta aagtaaagaa tccgaaatta tataacaatg
     4021 cgattagtcc aactgaacga agtttatgat tgcttaacgc tttgacacct tcccaaaggc
     4081 ctacttttct ctttggtttc tcagtttcag gtaaaagtac cgctagggct ataaaaccgc
     4141 aaaacatcaa aaatgctacc ccaaaaaatg gataacgcca tgaaccactg cccaagaccc
     4201 caccgactag agggccaaca gccattccgc agccaagtgc tgcttcatac atcatgattg
     4261 ctttttctgt gttaccaact aaaatactga cgatcgttgc taaagcagta gagacaaata
     4321 aagcattccc gacaccccag ccggcacgta acccaatcag cattccgata ttcccagata
     4381 atccgccaag accagcgaat acaacgatga taaacaatcc tgtcatcaat gtttttttca
     4441 aaccgatccg actagataag aaacctgtaa atagcatcac cacaccagtg actaacatgt
     4501 aactagtaaa taacagggtc gtttcagctg gcgtagcgtt taactgtgca gcaatcgttt
     4561 ttaagattgg gtctactaga cccactccca taaaagcgat catacaggta gctgctacaa
     4621 ctagcgctga acttttcccc tttgtttctt catttgtttg ttcttgtata tctatttgat
     4681 tctccaatat ttccatccct ttctctctat tagcatcata catgatttat tttacatgta
     4741 aatatgcaca tgtaaaacgt ggaaacttta ctgtgatata ataaagaaaa taagacgaga
     4801 ggtaggattt ttatgaatac tttagcctat atattattat gcgtgctttt atccaaacca
     4861 cgttctgggt atgaattgaa gcagctgatc acaatttttt gggaagccca tcacagtcag
     4921 atctatacaa cgcttgccaa gttagaccag cagggttatg tgaccgtttt ggacaaagac
     4981 gagcactctc aaaaaaagat ctaccacttg actgatgaag gacgtgctct tgtagaggaa
     5041 tggatcaaag aggaaactcc cgctcctacg caaaaagatg aatttctagc aaaaatctat
     5101 gctttttcga cgctcgataa aacaacagct actggactgc tattcactag agaacaacag
     5161 ctgaacaaaa tcatgcaaaa aaatcaaaaa aaattgaatg acctctcgct agacaagaaa
     5221 caagaatttg gacgttatgt tgtgatccag cgcagaatta tgctttgtca gcaggaactc
     5281 caatggtgtc agcaagtaag agaacaaatg cagcagtttt ttgagtaagg gataaacaga
     5341 acctttctag aaatatctat tgaagagcaa attatcttca ataaaagagc aatacagttg
     5401 gaaaagaaaa agcgatccga acgacaggcc aaaacctatc gttcggatca ctatttttct
     5461 agaactgcat tcaagagact atgacaaaaa gcatgccaag gccttttttc aaataacagt
     5521 gttccatcat tcattacttt gaattgcttt tagcacactt cttgaaagcg ggccaacgat
     5581 gatcaactgc aacggtaaag cggcaatcac attcattttc caagcattca tataagcaga
     5641 gaataattga tcaggtattc cttgttccat gatcacaccg aataaagaca tacatgtcac
     5701 cattcctaaa accatcaagc atgaaatagt taagatcatt tggaccttgc tttctggatt
     5761 tactggtagt ttaaaggcaa tttttttcgc cagcacaccg atgatgaaga catccaaaat
     5821 gaaagccacc acgaatccag gcactagacc aatcaacaaa cttgataaat gaagatcttg
     5881 atgcagccat aaattatacg cgctcatccc caagaccatc aaaaaacaca tcattgttgt
     5941 aaaaattatt ccttcttttt tatttgtagg caatctgtac cactccttta aatttgtcac
     6001 gaaaatcatc gtagcacatt taaaaatttc tcgtaagtac tttttttatt ttctgccctt
     6061 ttatttcata aacttcatca gccgtacgct cgacaaagta agaatcatga gaagtaaaca
     6121 aaatcgttcc cggatatatt tgcattagct tctctaaagc ttcgatcgtt gccatatcaa
     6181 taaaattagt cggttcatct aacagcaaca cattacttgg cttagtaaaa agcaaagcaa
     6241 tcgtcagacg ggtcgcttct cccccactta atgtacataa cggtttcgtc aaagtttcct
     6301 gagcaaaccc taagtgattc agcaaactac ggacaagcga ttctgaaaac tctgtttgtc
     6361 tcatgacaaa acgaatgatg gattcttcag atgtcatttg ataacccttt tgctgatata
     6421 cttgaaagct tactttcgga gagagaagga ttccttctcc ttgtttgcgg atatgttcta
     6481 ataaggttgt cttacctgaa ccattttcac cgacaatcgc gattttttta cctaaagaaa
     6541 actgaaaatc accatttacc agtaatgttc tcccacttct ttccaattgg acattttgtc
     6601 ccatgatggg atagcggctg tggagttcaa gagattttgg tactggaaaa cggatttgtt
     6661 tcatctgctg cggcttggtt gtttctccta ttttttggag tcttctctct aatgttttcg
     6721 cattcttttg gatggctttt tgtacactgt ctttttgttt agaggaagac aaccgatccg
     6781 gtctgatttg ttgctttttc tttttactgg acactttttc caacttttcc gcttgttttt
     6841 tcttttcttg gatcgatttt tccaatcgtt ttttctcttt catgatcgac tctgcttctc
     6901 gtagctgagt ctttttctcc aattcctttt gagtgagata ggcactataa ttccccgaaa
     6961 actctcggat ttctccgtct tttacttccc agattttcga tgctagctca tctagaaaat
     7021 agcgatcatg actaacaaaa accagcgtac cataataata acgaagctct tcaatcagtc
     7081 gatcaatact ttttctatct agatgggttg ttggctcgtc tagaagcatc ccttgttcat
     7141 aagaagaaag aagacgtatc aactcgatct ttcgttcttc ccctccgctc catttccgct
     7201 ctgaagtttc ttccagtctt aactggaaat gactggctaa ttccaaattt aggtcctttg
     7261 cttcagcgag ataggttaat tgaggcaggt agttgaaggt gatctctgtt tggattttcc
     7321 catgatcggg aaaaagttca ccggctatta gtttgagcaa agtcgattta ccgacaccat
     7381 tttctcctat gatccctata cgatcttgct gatagacacg aagagaatca atagatagtt
     7441 ctagctggtt gccaaagctg acttgtagat ttgttatatt tactgctaaa ttttccatat
     7501 gttttacact cctatttcat gtaaaacaac aattatcgat actaaaaaag agcagaaaaa
     7561 tctgtttgca aaaaaactgc atcacatctt ttcctactct gaacgaaata actatgacta
     7621 aatcaccaaa tctgtcatag ctttcaccta ttctactgtc gttcgaaaaa atggacatac
     7681 tccatccatg aaattgttgt ttatacatgt cttttgaatt tatttagttg ttattcaaaa
     7741 gttcgaaacg taatttcatc gatgcagtca tttttccgac ctcctttttt attaggttac
     7801 cacacacgat tcaaaaaaag caatagctac tgaatagtaa ctgaaaacct tttattcgaa
     7861 cgtagtgatc ttttctggtg ctaaacgaac agactcgtaa ccttcttcta ccgcttttcc
     7921 tattgataaa ataataactg gaacataacg gtctttatcc aaaccaaatg cctctgccaa
     7981 ctgatctgct tcgaatccac caattggatt tgtttcatag ccatgatcac gcgcaactaa
     8041 catgaattgc atagccgcta agctagaatc gactttgact acatcgttca tttcttctct
     8101 tgaaaaactc ttgtaataag gaatgatcgc agctaattgt tgatctctga cttcttttgg
     8161 catcttccct gattcgtaag cttggttata gatttcttcg ccatattcgt aacattgcat
     8221 atcgccaaaa atcatgacca ttgcagaaga agtatcattt tgtctcgtat tgaaacggat
     8281 caaaggacgc aagatttctt ttgcttcctc gctttcagcg accacaaagc gccatggttg
     8341 catattgaca gaagaaggtg cagtcgtcgc ttctttgatc atttccagca tttcttcgtg
     8401 tgggattttg tatgtttcat catatacgcg aacggatctt ctaccagtca taattgttga
     8461 aaaatcattt ttttgttcca ttattattaa cctctttcgt ttctgttgtt atattgcaaa
     8521 gttgggtatt caatttatct aaaagtgtta gaaattgttt catttctaca tcgctgaaag
     8581 ccggggatag cacggaacct gtatctttat ggtccatttc acaatgctcg attttcttct
     8641 gggctttttc tgttagcgct acatacactt cccggttgtt ctgaggattt ctctgacgcg
     8701 aaacatagcc atttgcttcc aataccttca gatgacgagt aactgctcct tgatcaatct
     8761 gcaacttttc ttgcaaactg ctttgtagca atggattttc ttctttcagt ctcatcatga
     8821 tctcgtatct agtaagactt ataccgatat tttcttcaaa gatctggacg attttttgat
     8881 cggctaattt gatttgatat aaaaggtgac taatctcttc taaagacata atttcctcct
     8941 ttctaataat tgactcgtca acgattgact tgtcaataat agaataaaaa aagaaaaaag
     9001 tcaaagaatc agctgtgtaa taaaaaaaac gctctcaata gaaaagtata gagaaaagaa
     9061 attttttgta gaatgaacgg aaaaaatgat aaagaaagca caacgctaaa accaagatta
     9121 ttgattctta tcaaactata gaagatagct cttctgcagt agaaaatttt acaccgccaa
     9181 aaatttccga aatcgccttt tgcaagaatc ccgtcgaata ttagaaattg gatcagtatg
     9241 cttaaacagt aataaaaaag ttatgacaag atgttgtcac aacctttttt gagtaaacgg
     9301 tgttcaaaaa cttgcatctg cggtaataag ttcaaccaaa caaaaaacat gaaaatctgt
     9361 ttttgtttgg ctgttcttac tacttgatgc agggcagctt tttcactacc tctttttaat
     9421 aaccataaaa tcctattcct tcatatctcc agcccatcgg tgttagccaa tctctttcct
     9481 tagcactcaa ggtatagtga tgggtaccag ttttttcatt tggattaaat agtcgataaa
     9541 ttggcttgga accaccagaa taaaaactta gaccttcata acgccaaccg atttttgtta
     9601 aagagttgat ctcagattgt gccattgtgt aatgatggtc tccactgtaa ggattgtata
     9661 aacggtagac aggttgtccg ctgtttggtg cttgccaagc aatcccttca tattcccagc
     9721 ctacagttgg cagataattt ttttctccgg cattcaatgt atataaatgt tctcctgtat
     9781 gacggttata cagacgatac atcggagaag ttgaagttgg tgctgtatca tactgtgtca
     9841 gattataggt ttcgattaca ttgttcaatg tagaaccata attaggcgca gtggcataac
     9901 gacctgttag ccaagccgta gcatctttgt aagagttcgt attgcttttc catgcaccgg
     9961 aatagtagta aacgcctggc tgaaaagaag tcgtcttcaa taccgttacg ttatcttgca
    10021 atgattcttt gtacgaagga tattttctaa aagcggcatt caccgtaatc cattgcccat
    10081 tcacatattc cgaagtaggc atcgtaacag attggccatt atagcttcct ttaatcccaa
    10141 atagattgta attaggggca gaagccaaag tactgttccc ccagccactt tcaacgatgg
    10201 cttgagcaat catgactgat gcatataagt cattggcact tgccaaactt tgtgcagttg
    10261 aggcaatact atcaatgaac gcttgttgag aattagcaga tgagcgagca taaaaagcat
    10321 agccagtata gttttcgaat tcagacaaat ctgcttgatg ggtatgtgtc aatgtttgcc
    10381 catctgtcaa ttggttcgtg atttcttctg cattctctgg attgttctga gcttctgctg
    10441 atgtactgtc ttcggtttgt gtttgttctg ttgtttcagt tgttggttct tcggttgaaa
    10501 ttgtattttc tgagacagta gctgactgca aagtactctc tgtatcgttt tcaattcctg
    10561 tttctgaaga actcgatgtc tgagttgcga cagtttctgt cgattcttca gctagcactg
    10621 ttgtgttggc aactgctaga tttcctaaaa gaataacgct aaataataaa ttctttgctt
    10681 tcatcttttt tctcctttga attatgaaac ttttcctaaa taacagtgta actaaaaaag
    10741 taaaagcaag tcaatgtcca tatttaaata acatttgttt ctttttgata aatttgcttt
    10801 ttctacataa aaagacgtaa atgaagaata ttcttctcca ttcacgcctt aaattgatgg
    10861 tcgtttgaat aactatttaa caagtaattc tctttggata ttatccgtta tggattatac
    10921 ttcctgtgtc gtcggacgga taatcatgtt actgacagac atccggtctg gcgtttctat
    10981 cgcaaatgct acagcagaag ctatatcttc ggaggtcaat ccccattctt tttgcgcttc
    11041 atgaagttca agagcagcct ttttgtcgct gatggtttga tataattcag tttgaacagc
    11101 tcctggagag atgatcgtag atttgatgtt attttgccgt tgctcttgac gtaatccttc
    11161 catgattgca cggacagcaa attttgttcc gcaataaacg gcggaatcag gataaacaac
    11221 atgtcccgcc acagaatcag ttgaaatgat atgcccagat ttttgttttt ccatgattgg
    11281 aagtacggcg gaaattccat ttaacacacc catgatgttg atatcaagca tattttgcca
    11341 ttcatcacgg tgaccttcaa ctaatggagc ggttggcatg attcctgcat tattgtacaa
    11401 tacatcgatt cgaccgaact tttccatcgc taaatcgata actgcttgga cttgtgcaaa
    11461 atctcttaca tctgctgttt gaatatatag ctcagcttct ggtaaagatt cttttattgc
    11521 ttttaggcga tcttctcggc gtgcagctat aactagttta gccccttttt ctgctaatag
    11581 cttcgttgtt gcttcaccga ttccgcttga agcgcccatg atgacaatca ctttttctga
    11641 taaatcattc attctattct cctcctgttt tgaaaaatct cttgcttata ctcactatct
    11701 cttatttcat cttactgttc aaataatccg ttcgacaggt ttgacgattt tggcagggat
    11761 tcctgcaacg atggtattgt ctgggacatc tttcgtaaca gttgcattcg ctgcaacaat
    11821 ggcattctcg ccaatcgtta cgcctggcag aatagttgct cctgctccga tccaagcatt
    11881 tttcttgatg tggattggct ttactgacaa gcctcttcgt tcttttggat cggttagatg
    11941 gttcaccgtg atgaggttga cacgaggacc aatcaatact gaatcttcta aacagatgcc
    12001 acctaagtca acgaacatca cttgacgatt gataaagaca tttttcccta tactaatatg
    12061 tttgccgaaa tctgtataaa aaggcagcag aacagtcacc gtatcatcta ctttgtcact
    12121 aatgatattt tctagccgtt tgcggatatc tgctggttca tggtacccag tattcagcgc
    12181 tgctaaaggc tcttcgtttt cagcttggat ctgatggatc acttcaaaga gcggatccgt
    12241 tactcgaatc tctttattga cgatttgttt ttcgatgagg cttgtcattt tgctccctcc
    12301 ttttatcctt actttataac ggacggctta tcatgtctaa tacttataaa acatacttaa
    12361 ctattccttt taactatact tagtggtatg cttttttaaa aacaggaggg aaaaaatgga
    12421 tattcgtata ctgacgtatt ttttagcagt agctagagaa aaaaatatca gccgagcagc
    12481 aaaatctctg catatcacgc aacctactct ctcaaaacaa ctgaaagatt tagaagaaga
    12541 acttggtgtg acacttttca ctcggggctc ccgagagata cagttgacgg attctggtcg
    12601 atatctttat tcaaaaggaa aagaaatctt gtcgttggta gaaaaaacgg cgaacaattt
    12661 aacaaatgac gcaatcatct ctggagaaat ctacattggc ggaggcgaaa ctaaagcaat
    12721 ctctttcatt tctgatagcc ttcattcgtt gatcaaggcg catcctgata tccagtttca
    12781 tttatatagc gggaatgcag atgatatcat agacaaactg gatagcggat tacttgattt
    12841 tggactcgta atcgaaccga caaacaaaca aaaataccag tcatttcgac tgccagccca
    12901 agatcgttgg ggacttttga tacatcgaaa tcatccttta gcaaagcaaa ccgtcatcca
    12961 tccgcaggaa ctgaaaactg ttcctttgat catttcccgg caaacgtcag tggacagtca
    13021 actagctgaa tggctaggtt tcagcataga tcagttatcg atcgtcggaa catataactt
    13081 attgtacaat gcttctttga tggtcgataa cgggactttt ggtgcattag ctctagatgg
    13141 gatcatcaat acttatggga cggaaaaaat attcatccca ctcgatccgc cattacaagc
    13201 ttcactgaat ctcatttgga aaaaagacca gcctctttct cctgctgctc gtaaattttt
    13261 agatgtccta acggagaaat acagttaagt agagtttttt tccttcgctt tcagcttttt
    13321 aacaacgaat aagataagca aaagatccaa cgattaaata acataagttt cttaatttat
    13381 ttcttccttt tatttctgat aattccagta aattactata attataaaaa aagaattgac
    13441 aaaacataac tgtctatata aaattgatta cgttaacaat aattcgtttc atgtgactag
    13501 atcatactag gcatgaaaaa tctctgagga acatggtttc cctaggaggt ttttcgtgtc
    13561 tatttttgtt tggccgcaat caactggtct agcaaacgtg aaaaccaaat cttcaaaaag
    13621 ggtgcgcgca tgaaaatcaa aaagatcttt aatcaaaatg cagtattggt agatgacggt
    13681 ggacaggaaa aagtagctat tggaaaaggg attggatttg acagaaaaag aaatgatttg
    13741 atttttgatc gtgatgtcga acggatattt gtcatggaac cagaaggtca aatcaagttg
    13801 caaaatttac ttaatcagat tgatgaacgc tttttatttg cagcagaaac gatcattgat
    13861 catgcggaaa ctgttttgat ggaaaagttg aatgaacatg tattgatcgc tttgaccgac
    13921 cacattgctt tttctgcgga gaacattaat aacggtatcg tcatccgcaa caaactttta
    13981 agagaaatcg aagtgcttta cagtgatgaa ttcagtattg ctcaatgggc agtagagtat
    14041 ctgacaaaag agcttgggat tccatatact tatgacgaag caggatatat cgcaatccat
    14101 attcacagcg gacgaagcgg tcggaacaat aaccatcgca gtatccgtga agtgacgatt
    14161 gtatctgaca tcatccattt agtggaatca gaattggaaa cagatatcca ttcagaacca
    14221 ttttcactga attattcaag actggtgaat catttacgac tgcttctaca acgaacccat
    14281 gcgcaacaat atgctgtttt agacacggaa atcgtagaaa tggtcaaacg aaaataccca
    14341 gaaagctata agatttctaa aaaaatccgt gtgttgctga ccaaagaata ccagctcgcg
    14401 atcacgaaag aagaactggg ttacttggcc atccatatcg agcgtttaag aagcgcaatc
    14461 gtacaaacaa atgttaacaa tcatgaggag gaaaaaaatt gaaagcatat ctacaacgaa
    14521 tgggacgttc ccttcaactt ccagttgcgg tcttacctgc tgctgcttta ctagtaggga
    14581 tcggaaactg gtgggcatct tatagtaacg acattgtcgc tcatttttta caagctggcg
    14641 gtaacgcagt tttaggtcaa ttacctttgc tgtttgccgt tgggttagca ctagggatgt
    14701 cagtggacaa atcaggtgct gctgccttgg caggtttggt cgcatttgaa gttccagtaa
    14761 atgttttaaa aactgattca gtagctactt tattgaatat caaaccagaa tcagttgatc
    14821 catcttttgc tcagatcagt aacgtattta ttggtattat tgccggattg attgcggcag
    14881 ctctttataa tcgtttccac gaagcaaaat taccgatggc tttatctttc tttagcggca
    14941 agcgtctagt gccaatcttg gctgcctttg tcatgttgct gatcagtgca gtgctactgc
    15001 tcgtatggcc tccggtttac tctgctttag tttcttttgc taaattcatt ttgagtttag
    15061 gctggggtgg tgcaggtctt tatggcttct tcaaccgttt actgatccct acaggattgc
    15121 atcaagcaat gaactccgtg ttctggtttg acgtagcagg aatcaatgat attggtaaat
    15181 ttttggcttc tgaaggaaca aaaggcgtaa caggaatgta tcaagctgga tttttcccaa
    15241 tcatgatgtt cgggcttcca gcaggagcat atgctattta cagaaatgca cgtccagaaa
    15301 acaaagcaaa aacagcttcc ttgatgctag cagcagcttt tgcttcattc tttactggtg
    15361 tgacagaacc acttgaattt tcatttatgt tcgttgcttg gccattatat gtcttgcatg
    15421 caattttcac tgggatttct ttagcagtga gtgcattctt ccattggaca gcaggatttg
    15481 cctttagtgc cggatttgtc gattacttcc tatctttgaa aaatccgatt gcgaatcagc
    15541 cattgatgtt gatcgtccaa ggattagtaa cagcagtgat ttactacttt ggctttaact
    15601 ttgctatcaa aaaattccat ctgatgactc ctggccgcga ggaagcagat ctttcagatg
    15661 acgatactgc aacgactaat tcagacaata aatatgcggc acaagcaagc aaaatctacg
    15721 ctgcattagg cggagccgac aatgtaactt cgatcgataa ctgtactaca cgccttcgtt
    15781 tgcaagtcaa agatacaggc ctgatcgatc aaaataaaat caaagccact ggcgtacctg
    15841 gtatgaagat catcgacgga aaaaatgcgc aagtcatcgt aggtacagaa gtacaattcg
    15901 ttgcagatga gatggcaaaa ctacatggtg gtgctgcagc acgacctgca caaacaaaca
    15961 ccgtggttaa aacagaaaca tttgccggtg aaacgatcga acaagatatt tacgctatcg
    16021 caaatgggaa actgattcca atcactgaag tttcagatga tgtattcgcg gaaaaaatga
    16081 tgggtgacgg ctatgctgta ttaccagaaa atggagaaat cttctctcca atcgctggta
    16141 cgatcacaaa cattttccca acgaaacatg ctgtcggaat ccaaacagat gctggtatcg
    16201 aagtcttgct tcatatgggc atcaacactg ttgacttaaa aggcgaacca ttcactttat
    16261 atgtagaaga aggtcaaaaa gtcgcaagag gtcaattgat tgctttagtt gatcttgccg
    16321 ctattcaatc tgcaggcaaa aacacggata tggtcgttgt attcacaaat ggcgacaaag
    16381 tccaaagctt agaaatcgaa cccgcacgtg atgtaaaagc aaatgataaa atcggctctg
    16441 tttccaacaa agcctgagcc atcgtcttcc ccagactttt ttatcgataa acaaacagga
    16501 gctgagacaa aaatcttgtc tcagctcctt tttccgaata aaaacggcgt tctcctgcca
    16561 actccgcggt aataagtcca gctaaacgaa acatatgaag agctattttt tatttgactc
    16621 ttcttattac ttgatgcaga acagcttttt cacaaaattt ttcttcatac tcaaaactaa
    16681 tcggcttttt cacaacctct ttatacatta ttcctgatcc aatatttctt ttaatgctgc
    16741 ttcaagagaa gttaatggtt ttcccaatac tttttctaaa tcatcagagg aaatagctaa
    16801 ctgatccttt ttaatatcat gttggatcgc taaaaacatg tcagctgctt cttgagggaa
    16861 tccggcttct tttagatttt cgacaaacgc tgcatcactt gcttcaataa tttctaactc
    16921 tttacctgtc gcacgctcta atgccttggc taattcttca tagtcagtta gattgccgga
    16981 taattctaag atttctggag agtcactttc tactagtact tttgctgcag cttctgcata
    17041 ttcacgtttc aatgcccaac ctgtttttcc atttcctgca gcataaacga attgaccagt
    17101 ttttaaagcc tgcccaatga taggtaattc attctctaca taccaattat tccgtaaaaa
    17161 tgtatgggaa atacctgaat ccttaatcat tttttcagtg aattggtgat ctggcgctaa
    17221 tatactgatc gagtgatcgg catctgcaaa acttgtataa gcgatataag ataccccagc
    17281 tgcttgtgct gccttcacta cattcttatg ttcggcttga cgatttcctg gaacgccaga
    17341 gacaaaaagc aatcgatcta ttcctttcaa cgcttctttc attgagtcta aatcatcata
    17401 atcaccaata cgtatattaa atccagcttc tttcagtgct tctccttttt cttcactacg
    17461 tactagtaca tagatttcat tttgtggaac catctttttt aagaattcca gcgcataacc
    17521 accaaaacct ccagttgctc ctgttactaa ataatgcata tttgccactc cctattcttt
    17581 ttatttgtta agatttacag ctgtacattt ataaattaca cctttatcat aaagagtgtg
    17641 gtaagataag tcaagcaaaa aaaatgaaag gatgacaact gatgaagtat tctctgcaat
    17701 ttagtgatgc aatccatgta cttgcctata ttgaaatgtt tcaagatacc cgatcacttt
    17761 ctagtgagat gattgccagt agtatcgaaa caaatgcagc aaatgtacgt aaaatcatga
    17821 gtcagctcaa aaaatccggt ttgatcttta caaaaaccgg aaaaccgcag ccttcccttg
    17881 ccaaaagtcc agatcagatt tctttactag atgtctatcg cagcatcgaa ggtaatacac
    17941 agcttatcca agtcgatcca aaaacaaatc caaattgcgt cgttggtgcg aatatccaag
    18001 aagctttaga agaaagctac gctcggctgc agaaaaaggc agaagaagaa atggctaaga
    18061 tcacacttga tagtctgata cataaaatag ccgttttgga aaaagaaaaa agaccagaaa
    18121 acacaggcct cgtagagaga tttttataat tctctctact gtgcctgcat ttttctgatc
    18181 ggaatcgtct acataaattc ttttattctc cgggggacaa aagccttgtc atgccccttt
    18241 ttcctctcta atgctcaggt caaagcgctg aagtcatgac gtcttgataa acggtgttca
    18301 agtagctgac cctcggcatt agttctgaaa aatgcaaaat atggaaagct atttcccatt
    18361 tttcctctct aatgctcagg tcaaagcgct actttcacga cctctatatt atcatggttt
    18421 attgactacc ggtatccaga tttctcccat atatttcccg ttggtaactc ccatttcgac
    18481 tacagcattt ggacccccaa cataggcgta atcttctact tctcttaaaa catgcccaaa
    18541 ggccgtattc gtcaaatcat ttcttaattc ttcaggcgat tctgctaaag cgcttacaac
    18601 aagatacgtt ccttttggga atgggatgat tcgcgtagtg tttggaacat cctgactcgt
    18661 ttgaacaccg acatagtaca tcattttccc gttatagggt tcgttgactg taaagaaata
    18721 atgattttca gcagcttgtt tcaatgcatc tattgttcca ttaccgataa ctttttgctc
    18781 aaatgcttgt ttctctgcag ccaaaccgac aaaatccgta ttgctactct tcaattccac
    18841 accaataccc gtcaccgtaa aatctggttt ttcttcgatc ctatactttg ccatttctcc
    18901 caccctttct attatattaa gttcatctta cataaaatta aagggaaatg acacgattac
    18961 gctctttttt ctagggatat ttttattttt taactatttt tttgctttca ctgcaatcca
    19021 aatctcactg taaacattac tgaaatcctc tccccatttt gtaaaagaga tctcgggagc
    19081 ttcgactaac ccataatctg aagaaggaag ccattctgca tagattttcc cccaagtatc
    19141 ttgtaaagtt tggggaaatg gccctttatt aggaaaaatg gcccaagtgt gttcttctat
    19201 agcaagcatt tctaaatcag cgtacggatt ttcttttgtc gtagcaaagc caatcatgtg
    19261 catcaaactg cccttttctt ccaagcggtc tccatcaaat ccataagaaa cgttgattac
    19321 ttgatttgga tataaatctc ctaacttatg catctcttcc ctttgagtct ccgtgatcgt
    19381 ctgcgctaac ttttgtattt cttgattgac tccttcaaat ttgacaggaa cttttttcgc
    19441 cacacctgcc acataaaact gttctttctt ttcgatacga aattccatcg attttcctcc
    19501 tctgacatca ataaaaaatg tcaagcgtgg aaaagatttt tgtatgccgt atctactgat
    19561 ttctgaaggc agatatcctg accaatcacg aaaagctctt gaaaaacctt cgactgtttc
    19621 atatccatat ctaaacgccg tttctgtcac tcttgatcca tctagaagat cgcgattagc
    19681 caaagccaat ctacggtttt tgatatagtc cattagcgac atccctgcaa taaacgtaaa
    19741 gatgcgtttt aattgatatt cagagattcc tgtattttcg gctattattt tataggaaag
    19801 tccttctgtc aaatgttctt cgatgtaatc gatcaattgg ttcagatctt taagcatatt
    19861 tatcttcctc cttgattgcc tttttatcat attatcaaag aaagtcaatt acttgatgaa
    19921 tcaggaacca atttagtcag atgtagaaaa tgttgtatgg atctaaagaa aattctcaag
    19981 cagcgtttcg taacggataa ttggtattat ctgcctatct atgttttacc tttagaaaaa
    20041 taaattttgg ggaagacaga ttctaagtaa acaaagcggt aaaaatatct tttattccct
    20101 tagttaatcc gcttactacc tactgtacaa agcaaattgt tgttcaaact gctgatcaga
    20161 acgctgcttg gatttttctt tgcgatacat catacctctt ttatcaattg gaacaattat
    20221 ttatttctaa caaaatcact tcatttttgt cagataaata aaaaacaaaa aaagttccat
    20281 ctttaaaaca cgctattctt ttactttttc tcacattctc taagaaaaga aaaggagaat
    20341 gaatttttga caattcgagc aaaagtgatt atcaaatatg taaaatcagc tacttttttc
    20401 ttaaacgttt ctttatacta aatataaata agtgtttcat gaaaggagaa ctagatgaat
    20461 aattggcttg agtattttcc tgaaaacgtt ttggaacgcg gttacagcta tcatctgcac
    20521 ggttttgtcc gtcatttgaa ctatacaagc aagtacttgt ccgcaacagt ttctggcacg
    20581 gaagattata aggtagtgat cacttgggat gaaaagacca acatgacttg tgattgtcta
    20641 tatgccatag aaggaaaaaa atgcaaacac atggctgctg tcttattcgc ttacgaagaa
    20701 cgtccaataa aaaaatccaa ctactcactt tccgaactct cgtcccttgt ttcctccgct
    20761 agttcctctc ttgtacggga gcttttaaca gagattctta tagaacatcc gcactttatc
    20821 gaacgcttca aagttaagat gccatttcat gcaataaatt attcagataa attaacaact
    20881 atcatccata aatacgatca tattatcaag aaaaacaaaa acaggaaaac agcgaagttc
    20941 atcatggaga tgcgaaagtt catccaagaa gcagttgaat cgcttattca gcaaaatgcc
    21001 tatcttcccg cattcgaatt gatcaatgaa gtgattgcta ctctcgaaac attctattgg
    21061 gagcctgagg atgaacgaac ccttcttctg atagaggact gttattattt atggaaagaa
    21121 ttattagcag aagcacctca tgcagaaaaa aggcaaatgt tctcttggtt cgtctgccaa
    21181 gtcgatcata ctgacgcctc ttattcaaaa cgctactcta taaaaatcct gaaagaagac
    21241 tttcgggaaa aagaattttc taaccaaaag aaaaaaatag ataaaaaact aaagaaacgg
    21301 taaagaaaga cgatttcaat gaaaaatagc aagtaaacga taacaaccat cctaaatcca
    21361 caagaaatac agcagacctt gctttaaaaa cacgaatcat gcaaaggatg aagtaaagaa
    21421 gacttaacgc gtacccaata ttagtcattt gactattagg tacgcgtttt gttttataag
    21481 tcatgacaaa tttccttcgt tgctggatag attttccggc attgttgata gattttatga
    21541 tacttttcta cattttgtaa gagaggttcc gctgcatctg tatattggat aaaattctga
    21601 caacaacttt caggtgtgtc gaaccaccct aaccctagtg cagcaatcat tgttgcgcct
    21661 aatgcaggtc cttcctccac acttaaacaa tagaccgttg catcgaaaat atcagcttgg
    21721 atttgtagcc atgcaagatt tttcgcgcct cctcccaccg aaacaatacg tgtaaatctg
    21781 cgacccgcta gttctttacg tttcaatccc accattgaca aatgctcatt aaaaatttag
    21841 aatattcaca agtttaacga caattatgaa aatttatgtc gtgctaaagg agttttgtta
    21901 ttggatgaaa agttagatat aaaaactgcg atcaaagaca cgttacctac cgttttcggt
    21961 tatatcggta ttggacttgc atttggtatc gttgggaaag ctgccggatt tcatccacta
    22021 gtcgtcacgt tgatgtccct gctggtctat gctggttctg cccaatttat cacagtcagc
    22081 atgcttgcta gtcacagccc attgctttcc atcgttttct cgacctttct agtcaattcc
    22141 cgaatgattc tgatgagtat gacgattgct ccttatttca agaaaaatcg gctgcttcaa
    22201 aatctgctga ttggcacatt gctgacagat gaaagtttcg cactgggaat gaataaattg
    22261 aactatactg gacaaaagtt aaactttcgt tggatgaata cagctaattg gatttcttat
    22321 ttgacgtggg tgggttcttc tcttgttggc gcacttttag ggaattttat tactgatccg
    22381 aaaaagtttg gtttggattt cgccatcgtc gctatgttta tcgggttact ttatcttcaa
    22441 gtcatttctg atagaaacac ctctaaacga ttacagttga tcctgattgg tctcactttg
    22501 attctggtat atgtcggact gatttttatc ccaagcaact tagtgattgt agttgtaaca
    22561 ctgattgggt gcggattggg ggtgtggatc aaacatgcct tcttttgagt ttacattgct
    22621 gactatttta ggttgtactt tagcaacttg gctttctagg gtcctaccat ttgtcttact
    22681 gaaaaaattc gatttaccgc aaccgttgct tgagtattta agttttgtcc cgatcgtcat
    22741 tatgtctgct ttatggttca gcagtctatt tacccaaaat atcggacatc tgccgcagat
    22801 caatctcgaa aatgctttgg cttcagttcc tacactttta gctgctattc tttcaaaaag
    22861 cttattggtg atcgtcttgg caggaattct ttccctttcg ttgattcgct tattataaat
    22921 aaaaaaacaa gagcttatgg caaattactg tcatgcgctc ttgtttttat caaagtcaaa
    22981 taagaaaaaa atcggcgtat tcttgcttat ccgttgaacg gaccactaac aacgactttt
    23041 cctaacatat gtcctgtttc tacgtctttg gttgcttgtt tcaagttctt cgcattcatc
    23101 ccatctgaat agaccttgct caatgtactg cggataatcc cttcatttgc taaagcagca
    23161 atgtgcgcta aagcttctcc ttgcgtttcg atttcatagt tatagtctgt tttagcgaac
    23221 atatactccc agtcgaagct gatagattgg tttttccaaa cacctatatc taacggctct
    23281 ttgatcccta cgatcgttcc cacatgtcca aatggtcgga caaggtgctt gatttgatca
    23341 aagtagcggg taatatcgaa taatacagcg acatactcta cttgatcgat tcctaattca
    23401 cgaaaacttt tacttaagtc gtgatgataa tcgattggat agtctacacc tgtttttttc
    23461 agccattcaa aattcttcgg acttgcagtc gcgtagactt ctaggccagc ccaatgcgct
    23521 agttgattca aaatcgaacc gacgcccccg gatccgttga tcactaagat ttttttcccg
    23581 cgattggcat tttcttctgg agttagacca aatttttcaa ataacaattc ataagcggtc
    23641 aatgaggtca acggcaaggc tgcagcttct tcatctgaaa gattttttgg tgcaagagcg
    23701 acaatacgtt cgtcaactag ttggtattct tgattgcttc ctgctcttgt tgttgtacct
    23761 gcgtaaaaaa cgcgatcgcc gacagaaaat ttttgcactt cgtcacctac agcaacaact
    23821 ttcccgactc catcaaaacc taagatacgt aaagcatttc ttatcccgtt atcttggcga
    23881 agtttcgtat cgacaggatt aacagatact gcgttgactt ttaccagaag atcccgtgaa
    23941 ctgggtacag gtatctgttc tttttcatca ataaagcttt ttggatcatc gatcggcaac
    24001 ccttcataaa aaccgactac cttcattaat tccttttctg acatacgaat tcctccttag
    24061 cttcatcaag ttggagcatc catacagtgt tcatttcaat atatatagta tgctatttgt
    24121 tccacctttt ttattcatca taattattct cttacttaaa gtaaactatt tgcacttttt
    24181 tggttaaaaa agaggttgcg acaagacttt tgtcacaacc tcttataaac ggtgttctcc
    24241 taccaactct tcggtcacaa gtcaaaatat tcatcaaaca tgagaagctg ttttttgaaa
    24301 ttgctccttg tgactcagag ttaaacggca ggctcacaac ctctgatata aacggtgttc
    24361 aatcaactga ccctcggtat tagctcaaaa aacgacaaaa tatgaagagc tatttttgtt
    24421 tggctgttct tattacttga tacaggacag ctttttcacg acctctaatt cacggtgttc
    24481 aagcaagaaa tcattgttca ctgaaaagcg ttggatgttc tttttcaaag gtctctaaga
    24541 tctctcgtac ttcatcagta gatttcgtat tcatcaactg aactcgcaag tcatttgctc
    24601 ctggaaatcc tttgatatag attttaaaga aacgatgtag tcctgtgatg gaacgcggga
    24661 tgatttctga ataatgatct tgcagatcta gttgcagacg aagtagtccg atcagttctt
    24721 tttctgtatg ttccctcggg tctttttcaa atgcataagg attcttgaaa atccctcggc
    24781 caatcatgat gccatcaacg ccatatttct cggccaattc ttctcctgtc tggcgatcgg
    24841 ggatatctcc attgatcgtg attagtgttt gtggagcgat ttggtcacgt aaagcaatga
    24901 ttttaggaat gacttcccaa tgtgcatcta ctttactcat ctctttacgc gtacgcaaat
    24961 ggacagaaag attggctata tcttgatgaa gtaggtgact gatccattct tccatctcag
    25021 acatttccgt atagccgata cgcgtcttga cgctgacagg caaaccgcca gctttagctg
    25081 cttcaatcaa ttgtgccgct acttctggac gaaggatcaa gccactgcct tttccacgtt
    25141 ctgctacatt agggactggg cagcccatgt tgatatcgat tccttggaag cccatttctg
    25201 ccatagcaat gctcatttct ctaaaaaatt ctggcttgtc tccccaaata tgggcaacca
    25261 tcggctgttc atcttctgta aatgttaatc ttcctcgtac actgtctttt ccatctggat
    25321 gacagaaact gtctgagttt gtaaactcag taaagaagac atccggtgct gctgcatgct
    25381 ttacgacatg gcgaaataca acatctgtta catcttccat tggtgctaaa acaaaaaaag
    25441 gctttggcag ctctttccaa aaattagtag tcatttattt gttactctcc attctatcaa
    25501 gcagtgaagt cgcaatcttt ctcaaaaaca ttgtcattat actaggaaac aaaaaaagaa
    25561 gcaaagtgaa cagcttaaaa gatgtttctg attgagcaaa aaaaagactg tgacaaaaat
    25621 cttatcacag tccattttcc ggaataaatg gtgttcaaaa gcttgcattt gcggtaataa
    25681 gtccaaccaa acgaaaaata ggaggagcta tttttgtttg gctgttctta ttacttgatg
    25741 cagaacagct ttttcacaac ctctccacta aacggtgttc aatcagctga ccctcggtat
    25801 tcgctcaaaa aacaacaaaa tatgaggaac tattttcccg tttgtttctc taatactcag
    25861 gtcaaaacgc tgatttcaca acctcttttc taacttctca atcctcttcc tttattgatc
    25921 aaataccaag taacggcgta caaaacaacg atgaataaca caagtacgat catcgatgtt
    25981 gctactggca catcgatcgt tccaaggaat ccgtaacgga aaccggagat catataaacg
    26041 atcggattga cttttgaaac agcttgccaa aagggcggta acatagagat ggcatagaaa
    26101 acgccaccta gataagttaa cggctgcaag acaaatgtag gcacgatcga tacatcatca
    26161 taagactgag caaaaatccc gttgagcagt cctgctaatg aaaacaagat tgccgtcatc
    26221 aataatgtaa tgatgaccat gaaccatgaa tacacatgaa gtggtacaaa gaacaaggaa
    26281 atgatcgtta cgagagagcc gacgagaata ctgcgaccca caccaccaat cacaaagccc
    26341 caaataatca catgcgtagg aacaggtgct accaagattt cttcgatatt tttctgaaat
    26401 ttttgagaga agaaggacga agaaacattg gcatacgaac tagtgatagc agacatcata
    26461 atcaaaccag gaacgatgaa ttccatatat gaaaaaccac ccatgtcacc gatccgccca
    26521 ccgatcattt ttccgaaaat aacaaaatac aaggatgtcg tgatcactgg cggaaccagt
    26581 gtttggaccc atatacgcaa atagcggttc gtttccttga tagctaaact ttttaatgca
    26641 gtaaaataaa gattaaacat ttttctcctc cgtatgatta ttttcttctg tgattttcaa
    26701 gaacagttct tctaaacgat tcgacttatt cctcatagaa agaactttga ttccttgtct
    26761 tgtaagctga tcgaagattc cgttcacccc ttgatttcgt tcaacttcga cagcgattgt
    26821 ctgctcatct tcaaatgtac aagcatatcc ttcaatcaca ggtttggtat catatggcgc
    26881 tagatcaaag ataaatgttt caaattgcag ttttgacaga agctctttca tacttgtatt
    26941 ctcgatcagt tcacccgatt ggatgattcc gatgttgcga caaagcattt cagcttcttc
    27001 tagataatgc gtcgtcaaaa tgatcgttgt accattttcg ttcaattctc gtagaaactc
    27061 ccacatctcg cgtctcaact cgatgtcaac tcctgctgtt ggttcatcca agatcagcaa
    27121 acgtggttca tgcatcaatg ccctagcaat catcagtctt cgcttcattc ccccggaaag
    27181 catacgtgca cggacatttc tcttttccca aagattggac tgcttcaaat atttttcgct
    27241 gcgtttcatc gcttctttgc gagagacacc gtagtagcct gcttgattca cgacgatttg
    27301 ctggaccgtt tcaaaagggt taaaattgaa ctcttgtgga accaatccaa tctgttgctt
    27361 tgctcgaact agatccttgt ccagatcata accgaagaca ttcacttttc cagatgtttt
    27421 attgactaaa gaagtaataa tcccgatcgt tgtagacttc ccagctccat ttggccctaa
    27481 tagcgcatag aaatctcctt ctttcaccga aagatcgatt ccccgcaatg cttcgacccc
    27541 acttccatag atctttttca aattatttat ttcaagcgca tttgccattt atttcctatc
    27601 tccttataga catgattctt aattgtatca tggaatacag cagatgaaaa aacaacttgg
    27661 cttccacaca aaaacacacc tgctaacaaa gaggagatgt gttttctagt tcatgatcat
    27721 cttcgataga ataactatct aaacaactct ggaactttta aaatcataaa agtaccgaca
    27781 gcaactacta cgatgacata tagaagatcc ttataactga ggttcttgtc ttttttattc
    27841 atgttaagct ttcctccgtc cttttatcga aagctttttt cttttatttt aaccgaaata
    27901 aataaaatgg aaatagttat tttcaatttc tgttttcgct gtttgtttta cggagaactt
    27961 tgccatggaa cgtgcgtctc gtcgctgatg ctaagttttt tcgataaaag ctgctagaag
    28021 gaagacagcg tggtttattt tgtatcgtct tttgctaaat cttccaagat catctggaca
    28081 gcagactcgt tcccattgaa ttctgccact tttttggcat caatgtactt cttttgataa
    28141 tcgtcttttt ggacccacgc atcatgcttc gtttgataaa gttaatcaaa cggataccac
    28201 tgacgctgtt catcgaacaa caaactttct ttttgggcct gttcaactat ggatttagga
    28261 taattcaaaa ctttcagcaa ggcaatcgca tttcgggtcc ttgaaggtcc ttcttttaat
    28321 ttgaaatcaa aactgattcc ttttccttct gttacttgtt cttcaaagtg gacattctca
    28381 caataattct ttaaaatctc tgtcaattcg atatcatgtg tggcgacaaa tgctaaagat
    28441 ggatactccg caagccaccg aacgactgaa gcggaagaag caatccgttc gatcgtattt
    28501 gttcctttta aaatctcatc tacgaagcag tagcagcgtt ctttggaagc cacttggtcc
    28561 agcaaacgtt tgatcgattt gatttctgaa acaaaataac tgtcgccttc aaataaatca
    28621 tcttcgattg ccatggaagt caacacatgt ccaggttgca gggtaaactg ttctgcaatc
    28681 gctgtttgga tcgtctgtgc tagaatacag ctgatggcta tacttttgac atacgtagat
    28741 tttcctgatg cattggaacc cgtgactaat gtattcttct gccagttgac tggattaact
    28801 accgcttctt ttaacaatgg atgatagata tctttcgctg tcacaccgcc ctcagtaaac
    28861 tctggtcggc aagtgatcgg catataggtg cgataattca aaacggctgc tgcaacttcc
    28921 aattctccca gcaattccca cagcttgatg gcttcagttg aatgcttttc taatttagtg
    28981 agaacgaaat gataggagat aaatggcagc atgaacatga tgttgacata ttcaaaaatc
    29041 aattctgctt cactcccgct cttcattcga aatgaaaaac ctaatttagg gatgtctctt
    29101 aatggtttag tttgctgttt cagctctttt tgaagtggtg tcgtcatttt tgctaactga
    29161 ttggcggctg aaatcgttga aaccaaataa ctcatgctat tcagctctgt ttctagtgtt
    29221 tgtttcttca ctagataata gaccgtattg aatactaagc caccgattgc tagatagatt
    29281 cctagtactt gccctaaaag aagcaaacct acacccacca gcggaagcaa gccaagaaaa
    29341 ataaataaac ctacgcgtcc aatcttttta ctttttccat ccgctagata ttgttttgca
    29401 aaattatgat ctttttttcc taaacgagcg aattgatatt ggattctttc tctcagttga
    29461 ggattttcct gataaaaagc aattagtttt tctaactgat aatcttctcc aaaatcaaag
    29521 ctacgtaaac gctgatacaa cgcttctgaa cctacactag aataggtact gttgatccct
    29581 tcaaatactt cgaacatatc caagtcatac caagtaagat cgtcgatttc actatcccat
    29641 gaacggaatt ttttctccgt gagccacgca tccttcaaac tttcttcttt atcaaatctc
    29701 ggttgataag gaatttttcc ccaattacta cgaactgttt cctttacttt caaacgtgaa
    29761 aaaatgtcta aaacaactaa taaaataaca actgcaataa taattaaaac gataaattct
    29821 ctttggccca tatccctctc cgttccttga tttgttagtt ttagtatagc actgttgcgc
    29881 tatttgaaaa gggggtacaa aaataactgt cttcgttacc taattcgtat caatcacacc
    29941 aaaataaaaa caccttccaa aaaagaagat gtctttaaaa cgagatacaa ttttttgtca
    30001 ttcacttcag gtaaattaga gccaattaaa ccctccgcaa cttgacggag atggagaaga
    30061 tgcccccgtc taatctagcg gtcatctctc ctcccattcc ttccaccaat ttttttgaca
    30121 ggtaaagccc gattccgcta gattccgtat ttgtgcggct ggtattttcg gtataaaaac
    30181 gctggaagac tttttcaaca gaggtttgcg ctcctggttt gataggattg gccactgtta
    30241 atcgaatgaa tgaatcctct tttgaaaatg tcacctttgc ttgtccatct gcataacgca
    30301 ataaattctg tatgatattt tgcagcactc gcagcaacat gagcggatct gctaatacag
    30361 ttgtatttat cgagtgttca gcgaacgcta cctcgatatt tttctcagaa aattgttcat
    30421 aaaaagagaa aaatacttct tccacctctt tagaaaaaga aagcgtatgg agatccattt
    30481 ctttttgctt cgtctctatt cgagcaagat cataaaactg atccgtcata ctgagtaatc
    30541 ttgcaacaga ttgtttgatg atcgttaaat acttctcccg tttctccgta tcctctacct
    30601 gttccagtaa ttgtacataa ccgttaatag cagtcaaagg agttctgaga tcatgagaaa
    30661 ggccgacgat cgatagtctg atgttttctt ccatttcttg aaatgcttgt cctttttgtt
    30721 cattttcatc cacccatgta ttcagtgcat ctactaggtc cattagattc ttttctcgaa
    30781 attcgactga aagacgacca ccatattctt gtcgttcaga aagagtggcg attttcttag
    30841 ttaatctctt gatttccctt tgcaggaaga aaataaaact tcccataaag ataacaacga
    30901 gaagcaacag ccacttaatc aaatctaaac cccactcccc acaccgtatg aatatacaca
    30961 gcatgtgcct ttgctagttt ccctcgaaga ttggaaatat gcatattcac tgttttctca
    31021 ctatcaaaat atggttcttg ccaaactgtt tcataaagat ttgctcgcga aaatactttt
    31081 ttcggatgtt ctaaaaaaag cttcagcaga tcgaattctc ttcctgtcaa atggacagcc
    31141 acaccattca catttacatc cctcgactct ttgtccagta agatttcctt gtaatagatg
    31201 cgttgcgaca gatcttttac ttggacagaa gtctgatgac gaagttggat gccgattcgt
    31261 aacaacagtt cttcgatatc aaatggtttc gtcacatagt catccgctcc tgctgccaac
    31321 aaggaaactt tatctctttg atcgcttttt gctgaaagaa cgatgatcgg aacattcgat
    31381 gtctgtcgga tttctgctaa caatgcttca cctgaaagcc ctggaagcat gagatctaga
    31441 aggatcaaat cgacaggata tgtgcttaac aataattttc cttccgtacc tgaataagca
    31501 tcaagaagtt tgtatctctg ttcaagaacc tcctccagta aagaatgaac agcttcatcg
    31561 tcttcgataa tcaaaatagt ttccagcagg aacacctact taatgtcaga tttttggaaa
    31621 ataaacattc ctagagctga tgataccacg attgttacta gactgaccag aatcattttc
    31681 ggtgcttgcg aataagttgc ttgttgcgca aagtccgtat acatgagcca cgtttccgta
    31741 aaccactccg ttagaaattc caagcctttg atttttgcga aaacaccggg aacgaaggcc
    31801 gttcccatga tataaagaaa agaaagaagc aaactgacac tggtacttct agtgatgaat
    31861 gtcaaagcca ctggaacaga taatgtcgct aatatcagca gaagcaatac aggaaatact
    31921 tttagaacat aatttccata gtttttgatt ccctcaactt caccaaatcc gaaagcaagt
    31981 gtgtaagcaa tcatggctag ccccgtaatc aaaaccacgc caagtgttgt gatcaaaagc
    32041 gtaacgagaa gtttcgacac ataataagtc attcgattcc gtcctaaaga taacgtattg
    32101 cgaatcgtac cgttttgaaa ttcttcccca atataaaagc acgcaaatgc tgcgaaagcc
    32161 agaagaatcc atgcagccat tttttccacc attaaaatcc ctgttgcacc gttgatgact
    32221 gtatccgttg cttggtttaa ttcattgccg tattttgtat gcatatacca ttcgttccaa
    32281 gaataaaaaa gcggaagtac gaccatcagt cctagtgaga cccacatttt acggctcttc
    32341 catactttta ttttttcagc agttaataga ttaaacatgt ggattgcctc cagttaattg
    32401 taagaaataa tcttccagtt tttgcccaac taggtggata ccatccacag gaacttttgc
    32461 ctctaccaat gcttgattga tggctgctac ttggtcaatt ccttcaaaaa gctggataac
    32521 acggctagat tggacaaaat aatccctata accaagagag tcgagaaccg ttactgcttg
    32581 ttccgtcgcc gaagtgtcaa tttttataaa ttgacgagat tcttgtgcca attcttcttt
    32641 cgataattct ttgatcaagt gtccttcatg caaaataccg tagtgagtgg cgatctgtga
    32701 caattcatct agcaagtggc tagaaagcaa gacggtgatg ccccgttctt ttaccaaacg
    32761 ttggatgatt tctcgcattt ctgccatccc tgctggatcc atcccattcg tcggttcatc
    32821 caaaatcaaa aattctggat tcgtgatcag tgcatttgca atagctaaac gctggcgcat
    32881 acctaaagag aaattctttg ctttcttttt ccctgtattt tccaatcgca tcattttcag
    32941 caggtcttcg atttctctat ccgacacacc gccgttcgct gcttggatac gtaggttatc
    33001 tcttgcagtt aattctggat aaagagcagg ggtttcaatc gattgaccca tacgtcgtct
    33061 tgctgcctgc aaatccgtcg ttccaaataa ttcgatcgaa ccttcatcaa tcgagatcaa
    33121 acccataata gttctcataa aggtagactt acccgctcca ttctccccaa tcaaaccata
    33181 gatcattcca cgtttgattt cgatcgatac tttatctaat gctttggcag caccgtattt
    33241 tttggttata ccagttgctt tcaagaccgt ttcatttcca aaatccatac ttatccttct
    33301 ttcttattga taagtgaagt atagttgacg ggtataaaga aagcagccag aaatgtataa
    33361 agaaagtgta aattttccaa ttgaatttca agaaaaatca gactgtctcg cagacgtggt
    33421 tgagttgtct gccaatcaaa gagcgcacca aacagtgcta atccaagtgg actggccacc
    33481 atggtaatcg ttgttatcgt tgacatcaga cgtccttgta tatgcgttgg aacttctcgc
    33541 tgaagccaga ccatataaaa agcaaaagaa aacattaatc cttaaaaacg gacttatgaa
    33601 aaataataca tgccgtttgg gatttacaat tagctaccaa ataagaggtt atgacaaaag
    33661 acttttcaca atcccttctt ccgaataaac ggtgttctcc tgccaactcc tcggtcacaa
    33721 gtcacaatat ttatcaaaca tgagaagcaa tttttgtaaa ttccttctta tttcttgaag
    33781 ctgaccactt ctgtcacaac ctcagaagcg gataaataat attgtttgat gatactttat
    33841 ctttttcccg tttgtttata gatcgaatga tattatagac caagaatcct acctattaac
    33901 atagctaaaa acacaaaaaa ggcactttcc aaattgtgga aagtgccaga aaagttagga
    33961 ttaaggtaac tgcgctcagc attgcttcgc ccagtacgat tctcgtaact cgtgaactcg
    34021 aacgataatc gcaattaacg ttttgagaac tgtgaagctt tacgggcttt cttaagacct
    34081 ggttttttct tcgtatcatt gaataagttt gaaaaacctt gatttataaa ggttttcccg
    34141 taaatatatt ttattatatt tgattaaatt tgaaatgttt gtgttctaaa atgtgttcta
    34201 atttaaaggc agtatatttc gtattttaga ttaaattttt aggctcattc attctcaaat
    34261 ccattctatt cagtcaataa aaattttaaa aagactttat aaacaaagat gttcaagcga
    34321 gcatcttatt tttttgtgct taatttgaaa ggagaacatt tcatgtataa aaaacaaaaa
    34381 aagaaattat gtgtattaac tattttgagt ttggctgttc aagcaattgt tggagcaatt
    34441 gctcctacga ttacttttgc tgatgaaatt acccatccac aaacggtaac cgtagaacta
    34501 gatttagcac atcaatatgc tgttgaagga acttttagtg atggtcgtcc aatgtcagaa
    34561 gtcactgttc cgcattatgc agtgtacaac ggctttaaac aagatatttt ctgtattgaa
    34621 ccaggagtcc cgatttacaa tgagttcact cctggctatg agaaaaaccc gttaccagat
    34681 atgtccgata aagccaaatt agtttccgtt ttatggaaaa aagtaggtac agatgcagat
    34741 actcatattg tggcacagaa gatgatctgg caagaagtta atgggtatac ccttcactca
    34801 ataaaacgat cagatggtag tgccgtaaat atcgcagcca ttgaggcaaa aataaatcaa
    34861 gccattgacg attatcagaa gaaaccaacc ttccataata gtacggctaa aacagtatta
    34921 gggcaatcga ctactgtgac ggatacgaat aatctgaatt tgtcagagtt cgatgaggtt
    34981 gtggaaaatg ccgcaaacat tgattatcgt gtgaatggta atcagttgat aatcacccca
    35041 aatgccaatt caaaagaaag tggtgtgctg acgcttaaga aatctgctgg taccggaaca
    35101 cctgtcgcct ataaaaaggc tggccaacag actttaatgg ccggggcgat tgataaacct
    35161 aatacctaca caatcaaaat tgatgtggaa acggagggtt ctttaaagat caaaaaagtc
    35221 gataaagaat cgggtgatgt tgtaccagga acggttttcc atttagactt tggaaaaact
    35281 ttacctgcaa aagacgtgac tactgacaaa gaaggcattg ctacattgga tgagattcct
    35341 catggtacaa aagtaaccat taccgaaaaa tcagtaccgg cgccttatac gattgatacc
    35401 acccccatga ctaccaccat taaagcaggc gaaaccattt acgtgacttc aaaaaacgca
    35461 cgggaaaaag ggcaaatcat tctggataaa agcggagttg aaacagggag tgatctttgg
    35521 aatgacaatt attccttagc tggtaatacg tttgccattc gcaaagacag tccaaccggt
    35581 gaaatcgttc aagaaatgac cacggatgaa aacggtcatg cggaaacacc aaaagagatt
    35641 gctaatgcgt tggaattggg aacctattat gtgaccgaaa ctaaagctag tcatgggttc
    35701 gtgaatacgt tcaaacctgt aaaagtcgaa ttgaagtatg ccaatcaaac cgtagccctt
    35761 gttaccagta acgtaaaagg gcaaaatcaa gaagtcactg gcgaaaccac tttgacgaaa
    35821 gaagacaaag atactgggga taaagcacaa ggtaaggcag tctttgaggg tgctgaatac
    35881 accttgttta ctgctaagga tggaaaagct gttaaatgga gtgaggcttt taaaccagag
    35941 ttagcgaagg gaacgaaagg ttctgatgaa acggtcactt tgactttaga cgaaaagaac
    36001 caagttgccg ttaaacattt agctatcaac gagtattatt ggcaagaaac aaaagcgccc
    36061 gaaggatata gccttgatga aacaaagtat cctgtatcca tcaaaaaggt ggatgatagc
    36121 gaaaaaaatg ccgtgattac ccgaaatgtt acggcaaaag aacaagtgat tcgctttggc
    36181 tttgatttct ttaaatttgc tggatcagct gcgggcacta ccgaaactgg cttcaatgat
    36241 ctgaccttta aagtgtcgcc attggaaggg accaatgaaa tcacaggtgc cgaagatgaa
    36301 gcgaccacag cttataacga gcaattaggg tttgatggtt atggcaagtt tgaaaatctt
    36361 ccttatgggg attatttact tgaagaagta gaagccccag aaggttttca aaagattacg
    36421 ccattagaaa tccgttctac ttttaaggaa aacaaagaag actttgtgaa gagtgagtat
    36481 gtctttacca ttaccgaaca agaccaaaaa caaccaatta aaacggtgac cgttccttac
    36541 gagaagctga cgaacaaagc attttctgtt agtttgaacc gtttaatgct ctatgatttg
    36601 cctgaggaag aagatagttt aacttctctt gcgacttgga aagacggaaa caaagaatta
    36661 acttctctgg attctaccga gctagttgat aaattgagtt acaatctaca tgaaatcaaa
    36721 gaagactggt atgtggtagc gcaagccatt gatgtggatg cgacaaaagc tgcccaagaa
    36781 aaagatgaaa aagccaaacc agtggtgatt gcagaaacaa gcgcaacgtt ggcgaacaaa
    36841 gagaaaacag gaacttggaa aattcagcat aaattaaccg ccgaacaagt tttaaataaa
    36901 actattgtct tattcaacta tgtgtatgaa aataaggaag cttttgaagc aggcgataag
    36961 ccagtagcga aggatgttag cttgaataac caagcccaaa ctgtcagttg tacagtggaa
    37021 caccatgttt ctatccaaac aaaggcccac ctagaaaacg gttctcagac ctttactcat
    37081 ggtgatgtgg tggatatgtt tgatgatgtg tcgattactc atgatgtact ggatggctca
    37141 aaagaagctt tcgaaacaat tctgtatgct ttattaccgg atggtacaaa caaagaaatt
    37201 tggaaatctg gcaagattga ttatgaagta aatgataaag aattcaccaa aactgtgctt
    37261 gcgaaaaaag tagataccgg aaaatatcca gaaggaacca agtttacttt tgcggaaatc
    37321 aattacgata aagatgggaa tatcaatgga aaacacaatg aagatttgaa agaaaaatct
    37381 caaaccttaa caccaaaaga agttccaacc atactaagta cgccgaaaca accggaaaca
    37441 ccaactgttc caagtggctc tcaagaatct agtcccacag tgaagacctt ccctcaaacc
    37501 ggggagaaaa attccaatgt tttactgttc attggtttta ctttgatttt tgcgacggcg
    37561 ggctattatt tctggaatcg ccggaactaa ggtgatgaga tgatacaagt aaatcctaag
    37621 aaccaacgtg cgcgaccgcc tcctaaaaaa gtagtcgcgc tatttttgta tccaaaacta
    37681 acaatgaaaa gaggaaaaga agatggaatt gaaatttgtc gtgccagata tggctgaaac
    37741 ctttggaaag ttaagttatg ccggtgaagg agaaattata acagaaggat atggacggaa
    37801 tactacggtg attggtcgca gttatcattt gtattccagt aagcaacggg ccgatgatat
    37861 tgaggtggta gtagctgcgg aagccggtga aaaggatttt gatcaagacc aaccgctaaa
    37921 agccgtgaat ccccatttgg ttgccaaagg ctatgagatt gagaatcgtg ggtttactga
    37981 ttacgtgttg tatgttgatg atttagtgaa agcataggag gaaaagaaga tgagattaac
    38041 agaaggtatt gtcgtagatt caggtttaac atttgggaag ttgcgttttt ccgcattacg
    38101 ccgagaagta cggaagcaaa atgaggatgg aacaattggt aatgaggtaa aagaacgaac
    38161 ctataattta aaatcctctg cacaagggcg aatgattcaa gttagtatcc cagctaatgt
    38221 acccttacgc gagtttgctt atgatgcaga ggtagagttg gtcaatccaa tcgtggatac
    38281 cgttgccaat tatgtttttc gagaaggaac taccgttaat tggttcatta aggcggatga
    38341 tttagtcttg aagcggcagc cgaatcaagg aaatcctacg aaccaaaatg aaagaaagaa
    38401 ataggtgaag attatggaaa ttaatgttga acatatttta gattgtctcg atcaatatgg
    38461 aaaaggcgag ctgactgagg aacaactcgt taaagcatta acctctgatg aaaaaatgtt
    38521 tctgattata catcaaggtt tacttggaga aaatagcgac agtgaagaag actctgatgt
    38581 actagactgg ttaggagaag aagagtcttt ctttatagtt gtcgaagtaa atgaaaactt
    38641 atgtcggcag gcagaatcca ttttggaaga aattggtgtg gagatgccgg atgctattga
    38701 aggcttttta aatcaattgg ttgaaacgaa acaactcccc tcctcgtagc agtaaatgac
    38761 tagcataact ttttgaaatt gcctaatgaa atcctattgg gctttttcga ttggaggtgg
    38821 aaaccatcaa aatgtacaaa ggacatcgca tacgcgcagg agatcagcac ttggtttatc
    38881 actttgttct tggctggcta ctcgctctgt tcatcggttg gatgagcgtc ttttattttc
    38941 aagagtttag acaatttgat atttctaagc tatcactttc tactattgaa atcgtttggt
    39001 ctataaaaga tttagtatgt ctgcttggaa gtctcgcttt ttcaggagct atgatattgc
    39061 tttatattca cttttttctt gatcattggc gaagcttgtg gcatcgacaa aagctagctc
    39121 ggatgattct agagaaccat tggtatgaag tgaaacaaac tcaaagtgaa ggctttttca
    39181 aagatttgaa tagtagccga accagagaga ctatcagtta cttccccaaa atctattatc
    39241 gaatgaaaga tggattactg tctattcgcg ttcaaatttc acttgggaaa taccaagatc
    39301 agcttttaaa attggaaaag aagttagaaa gcggactgta ttgtgagtta gtagaaaagg
    39361 aactcaaaga ctcctatgtg gagtatactt tactgtatga tatgatcgcc aatcgaattg
    39421 ggatagacga agtagtggca gaaaatggga ctctgcgatt gatgaaaaat caagtatggg
    39481 cttatgattc tttaccccat atgttaatcg ctggtggcac gggtggaggg aagacctatt
    39541 tcctcctcac cattatcgaa gcactattga agtcagatgc agaattgttt atcctcgatc
    39601 ccaaaaatgc agatttagct gatttaggta cggtgatgcc tcatgtttat tcacaaaagg
    39661 aagaaatttc tgcttgtgtg gaagatttct atgaacgtat gatggctcgt agcaaggcaa
    39721 tgaaagaaat gtctaactac aaaacaggag agaattatgc ctatcttgga cttccaccca
    39781 actttttaat ctttgatgaa tacgtggctt atatggagat gttaacgacg aaagaaagtg
    39841 cggtgatttt gaataagctc aaacaaatcg tgatgttggg tcgtcaatct ggtttctttc
    39901 tgattttggc ttgccaaagg ccggatgcga aatatttagg agacggaatc cgcgatca
//
LOCUS       DACKAC010000019        39108 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.019, whole genome shotgun sequence.
ACCESSION   DACKAC010000019 DACKAC010000000
VERSION     DACKAC010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39108
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.019"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(55..402)
                     /locus_tag="I0B46_001049"
     CDS             complement(55..402)
                     /locus_tag="I0B46_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083363.1"
                     /translation="MFKVYFPFQWLFKGILLLLLGLILFGSYLTIKARYFPEEKPVKI
                     VTKDLKMGETYQADGVEITFKKVELTEKRNEFAQHDRVLQVTYTMKNDTENPIRPQQW
                     SIENKRLFVKSCW"
     gene            complement(395..697)
                     /locus_tag="I0B46_001050"
     CDS             complement(395..697)
                     /locus_tag="I0B46_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083364.1"
                     /translation="MSDPYIKVYCSKETKTIFDSFYEQNKGIFGTKANMFRVMVSNLP
                     VLANQSNVKLSNPESKGIEQKISEIESIVVGKLDEIDQKLSYYLKNKCKTEEGKDV"
     gene            complement(913..2304)
                     /locus_tag="I0B46_001051"
     CDS             complement(913..2304)
                     /locus_tag="I0B46_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAR2083365.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDNSEE
                     ALQEMNLVRTRMNKSEDIAQAFHVIHSFDKNISKELSIEKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNIDMETCKRLRIDPKDLERMRKINDEVLVENGLEPVDNQY
                     YENLEKRTNLYSNDPSKLKNGKINYQEFVQNAIEATLENCEVNSFVRFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQDVNFYERRDELITLGREKQSRKEKSQYVERTFSARKLGK
                     KYSFESVFERLEKNLEETHMNNFVDLDTEQIIALASGVDYKTGELNVPFVELDSIAQK
                     QFSDHEGAKKIIKYDDFLREVDRHYANTISLEDDQEYTIKLHTDVNPRTLRSTKSKTI
                     LVKKTDQKELFLPAEGFKNCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQRRQRDFEMDF"
     gene            complement(2289..2900)
                     /locus_tag="I0B46_001052"
     CDS             complement(2289..2900)
                     /locus_tag="I0B46_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HAR2083366.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVALIKQLNQIGNNLNQAVKALNIIAKYFREQSENKKEK
                     SLHFSFDVSRYNIFKDELANSIAKKMMISYPDDIFLESNRSTSSFLKLSEVEKDYFQY
                     WLEKQTRPDTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            complement(2912..3100)
                     /locus_tag="I0B46_001053"
     CDS             complement(2912..3100)
                     /locus_tag="I0B46_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083367.1"
                     /translation="MQLILQISTQDFLELAELCDSVKKATNEKITVTNYIELMIQEEL
                     MMKNEIESNFKCCSLEKM"
     gene            complement(3114..5651)
                     /locus_tag="I0B46_001054"
     CDS             complement(3114..5651)
                     /locus_tag="I0B46_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083368.1"
                     /translation="MKIIKKKIANRKTNWLPVIFLVLIAVILFTSNIAEGSIFNWFDD
                     TESQTEFLTNPLYQNYLKKYNGNFYSQFGMWIGWSVIKSMFAVVDGIQNMIPEVLDLF
                     SFVESTGLNKVYQAVLSTIVVGLMILSLVIIGYKMITGKGTVDLKSVGMNVVMSISLI
                     LLMPTLIHSGIEFSKTFYNDTTTITNSDEGVAWSLIEQGVTDLAYINKTDQYSMIDKT
                     NDRNNLTKENFWKTDLTQVLTDNVLDKMEKENPIADNLRYELVENSDNEFVATKFSDN
                     FLSTFVEGIKSGYYRYQANLWGISFGLIALAVAYVFSAFIIITAILELAFKRVLGVLV
                     FATDLETGQRSKQVLSDILQCYLTVGFQGFGLSMFAMFVNYLGTGSGSSVNPFIKAVA
                     YVCAVIVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGTGGMNRSKGGKN
                     SGGNDQAEGEERKPERNFGQSLSQKANKTGKTLGYARERGLSGLASDGATMASERATK
                     PFKSMRDMANDTKNKFKEGLDDGTVYAINKNSKPIVAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNINPASAIQQKVKQDIEERKNAMHGQAKS
                     AEVLINQKRQEAKYTPEAMNREEMLRKRVEGRTGANMDEKEALTKERIQEAKNSNTGL
                     EKSVKENLQNSTSSQDRRSVDVRENLQNTTSSQNGNRTVYVRENVQSSGTSQPRTVDV
                     RENLQNTTSSQNGNRTVDVRENVQSSASNQPRTVEVRETRAEASQGDKTQKINIIEER
                     KERPNEKVIVEQEVKEKKRPRFTYENNPLFQDISDKPNPLFDSLFKK"
     gene            complement(5638..5856)
                     /locus_tag="I0B46_001055"
     CDS             complement(5638..5856)
                     /locus_tag="I0B46_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083369.1"
                     /translation="MIENHLVDKIHCLDQNADTLELTESICSNTIVLDLKAVNDDYVT
                     HIIIDRKIAQKLSESLRKWAEGGNNENN"
     gene            complement(5858..6901)
                     /locus_tag="I0B46_001056"
     CDS             complement(5858..6901)
                     /locus_tag="I0B46_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAR2083370.1"
                     /translation="MNEKEVESLVEKQGYSNSDIYAELYYQYPIFLNVNEKYKKMKLS
                     SKLVYMLLKDRNRLSLKNNLYDENQKIFLIFEIKNLVEISGMSKNTILSALDELQKVK
                     LLVKRKNGFNKNKMQMFPNFYYLLKPVLCKDDVYKINKITHQENSQKNLIVQNLNYGT
                     NQYSVDSSKFELCDTSMKTRDKNNFADSSKNELELYKSYKDSKDIKESHNDDLFESLS
                     TKNNKLDEELIETFIEENLLKQMWGESIVNCLKLASNSDYRQFDNWYKKFNYAIKSAE
                     KEQKVQLKIIYEICNNSYFVDHVREQLAMTLRDLIRRAKTDHRIKQKNNYIFTSLKNK
                     ALELIKLQKKEGI"
     gene            complement(7056..7382)
                     /locus_tag="I0B46_001057"
     CDS             complement(7056..7382)
                     /locus_tag="I0B46_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083371.1"
                     /translation="MASWLIEEIENERRKIMDAGITVMLDKQQTNQLKNYVFEMTKEA
                     IDQARIDTGLERPFLKGKEMAKYLNVSYTTFLKFKRMGLPVILLEKMELFSKEECKKW
                     ILSHQI"
     gene            complement(7390..7593)
                     /locus_tag="I0B46_001058"
     CDS             complement(7390..7593)
                     /locus_tag="I0B46_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAR2083372.1"
                     /translation="MITLNDQFIRSLRRHRADLILTKNDAAKLIGINRKTYVKIENGS
                     KESIRASTYQKLVNWLLNDLKSK"
     gene            7668..8318
                     /locus_tag="I0B46_001059"
     CDS             7668..8318
                     /locus_tag="I0B46_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAR2083373.1"
                     /translation="MSIKTLLNLWFLCYNSLEFTIGGVTLNRIKFYIDSSKKTIKQIS
                     TETGINYTTLTNYYRGERNPRNTETWNILADYFHVPVSYLMGLDETNSIEQPNLDRIK
                     TARLEKKDKIVKNIASLDSDYFTELSNLYFSMTSLLEVKLQEDDSDFLFLKSILEDSF
                     EYTILFAKQKKFINKALNLNIDERKELESELFGNLTQVQVNNLKREITARLISKLQ"
     gene            8373..9539
                     /locus_tag="I0B46_001060"
     CDS             8373..9539
                     /locus_tag="I0B46_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAR2083374.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
     gene            complement(9628..10020)
                     /gene="rpsI"
                     /locus_tag="I0B46_001061"
     CDS             complement(9628..10020)
                     /gene="rpsI"
                     /locus_tag="I0B46_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAR2083375.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(10036..10479)
                     /gene="rplM"
                     /locus_tag="I0B46_001062"
     CDS             complement(10036..10479)
                     /gene="rplM"
                     /locus_tag="I0B46_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAR2083376.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(10998..11465)
                     /locus_tag="I0B46_001063"
     CDS             complement(10998..11465)
                     /locus_tag="I0B46_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAR2083377.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(11585..13123)
                     /locus_tag="I0B46_001064"
     CDS             complement(11585..13123)
                     /locus_tag="I0B46_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAR2083378.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            13203..13889
                     /locus_tag="I0B46_001065"
     CDS             13203..13889
                     /locus_tag="I0B46_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAR2083379.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(14090..14617)
                     /gene="msrA"
                     /locus_tag="I0B46_001066"
     CDS             complement(14090..14617)
                     /gene="msrA"
                     /locus_tag="I0B46_001066"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAR2083380.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(14701..15378)
                     /gene="pgmB"
                     /locus_tag="I0B46_001067"
     CDS             complement(14701..15378)
                     /gene="pgmB"
                     /locus_tag="I0B46_001067"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAR2083381.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(15375..16505)
                     /locus_tag="I0B46_001068"
     CDS             complement(15375..16505)
                     /locus_tag="I0B46_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAR2083382.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(16505..18688)
                     /locus_tag="I0B46_001069"
     CDS             complement(16505..18688)
                     /locus_tag="I0B46_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAR2083383.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(18685..19710)
                     /locus_tag="I0B46_001070"
     CDS             complement(18685..19710)
                     /locus_tag="I0B46_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAR2083384.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(19755..20582)
                     /locus_tag="I0B46_001071"
     CDS             complement(19755..20582)
                     /locus_tag="I0B46_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAR2083385.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(20579..21646)
                     /locus_tag="I0B46_001072"
     CDS             complement(20579..21646)
                     /locus_tag="I0B46_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAR2083386.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(21672..22508)
                     /locus_tag="I0B46_001073"
     CDS             complement(21672..22508)
                     /locus_tag="I0B46_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAR2083387.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(22505..23374)
                     /locus_tag="I0B46_001074"
     CDS             complement(22505..23374)
                     /locus_tag="I0B46_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAR2083388.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYDTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(23444..24727)
                     /locus_tag="I0B46_001075"
     CDS             complement(23444..24727)
                     /locus_tag="I0B46_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAR2083389.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(24741..26426)
                     /locus_tag="I0B46_001076"
     CDS             complement(24741..26426)
                     /locus_tag="I0B46_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAR2083390.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            26701..27726
                     /locus_tag="I0B46_001077"
     CDS             26701..27726
                     /locus_tag="I0B46_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAR2083391.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(28321..31974)
                     /gene="rpoC"
                     /locus_tag="I0B46_001078"
     CDS             complement(28321..31974)
                     /gene="rpoC"
                     /locus_tag="I0B46_001078"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAR2083392.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKSVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(32053..35670)
                     /gene="rpoB"
                     /locus_tag="I0B46_001079"
     CDS             complement(32053..35670)
                     /gene="rpoB"
                     /locus_tag="I0B46_001079"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAR2083393.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            36398..37366
                     /locus_tag="I0B46_001080"
     CDS             36398..37366
                     /locus_tag="I0B46_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAR2083394.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            37384..38289
                     /locus_tag="I0B46_001081"
     CDS             37384..38289
                     /locus_tag="I0B46_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAR2083395.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            38286..39092
                     /locus_tag="I0B46_001082"
     CDS             38286..39092
                     /locus_tag="I0B46_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAR2083396.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
BASE COUNT        11038 a         8209 c         6924 g        12937 t
ORIGIN      
        1 atgcggcgca cagaacgcgc cgcatatcgg actaagtttt attcaaattt gtcttcacca
       61 acacgatttg acgaagagcc ttttattttc aatcgaccat tgttgcggac gtatcggatt
      121 ttctgtatca tttttcattg tataggtcac ttgtaataca cgatcatgtt gcgcaaattc
      181 atttcttttt tcggtcaatt caaccttttt aaacgttatt tcgaccccat ctgcctgata
      241 agtttcaccc atttttaaat ctttagttac aatttttact ggtttttctt ctggaaaata
      301 tctggcttta attgttagat agcttccaaa aagaatcaat cctagtagca ataaaagaat
      361 tcctttaaag agccactgaa acggaaaata cactttaaac atctttcccc tcctccgttt
      421 tgcatttgtt ttttagataa tacgataact tttgatcaat ttcatccaac tttcccacca
      481 caattgattc tatttcagaa attttttgtt caattccttt gctttcagga ttacttaatt
      541 ttacatttga ttggttagct aacactggta aatttgatac catcacacga aacatattgg
      601 cttttgttcc gaaaatccct ttgttctgtt cgtaaaaact gtcaaaaatt gttttagttt
      661 cttttgaaca atacacttta atatacggat cagacatgtg tttttactcc ttatcttttt
      721 ttatttaggg agtctgtagg gagcttgctc cctcagaggt tttccccatc taacttgcca
      781 gaaagtagcc ctgcgtaagc aagggcggat tgactggcaa gtagatgggt agctggcgta
      841 tgccctcaaa accgcctctt tccaattccc tccgtatcga tgggaaactg gaaagtctac
      901 tggctaattc ctttagaaat ccatctcaaa atctcgttgt ctcctttgct gattttcaat
      961 catttgttgc gtgaactcgt aatctttttt agcacgatac attttcacat cgtcattcat
     1021 tatgcgactg atttgatagt gaatctgttt agcagaaggt gtatgagaac aatttttaaa
     1081 gccctcagct ggtagaaata attctttctg atctgtcttt ttaactaaaa ttgttttact
     1141 tttagtacta cgtaaagttc taggattcac atcagtatga agtttaatcg tgtattcttg
     1201 atcatcttct aaagatattg tgtttgcata atgcctgtct acttctcgca agaaatcatc
     1261 atatttaatg atttttttag caccctcatg atcactaaac tgcttttgtg caatggaatc
     1321 taattccaca aacggtacat ttaactcccc agttttataa tcaactccac tagctaacgc
     1381 tataatttgt tccgtatcta gatcaacaaa attattcata tgtgtttctt ctaaattctt
     1441 ttcaagtcgt tcaaaaacag attcaaatga atattttttc cctaactttc ttgcactaaa
     1501 agtacgttcg acatactgac ttttttcttt tctagattgt ttttctcttc ctaaagtaat
     1561 tagctcatct ctacgctcat agaaatttac atcttgtttg taaagaacat agccaagttt
     1621 gttatttgtt tttgaaaatt gataaacttc gatgttgtat tcttttgcta aatgatccga
     1681 aaaacgcaca aatgaattta cttcacaatt ctccaacgta gcttcaatcg cattttgtac
     1741 aaactcttga taattaattt ttccattttt cagtttactt ggatcgtttg aatagagatt
     1801 cgttctcttt tctaaatttt catagtactg attgtctaca ggttcgagtc cattttcaac
     1861 aagcacctca tcgttaattt ttcgcattcg ttctaaatct ttagggtcaa tacgcaacct
     1921 cttacaggtc tccatatcaa tattattaat aacaagatga ctatgaaaat gatctttatc
     1981 attgtgactt gcaacaataa tttgcgaatt tggaaaagct ttttctgcaa attcgacagc
     2041 tatttcgtgc attttctcaa tacttaactc tttagaaata ttcttatcaa acgaatggat
     2101 aacatgaaat gcttgtgcta tatcttcaga tttattcatc ctggttctta ctaaattcat
     2161 ttcttgcaat gcttcctcac tattatcact acaatttatt cctgagactg caacgcattt
     2221 ttcttttttt gaaggattag ctgcatattg taatagcgag gataaatttt tcttgcgtcc
     2281 tttcgatgtt atagtagctc ccaaagttgt ttcacctcat cttttaaact tgcaataatt
     2341 tgaatcgagt gaattaaatt ccggtcaact ttttgggaat actcttcttt cgtatcgggt
     2401 ctagtttgct tttctaacca atattgaaaa taatcttttt ctacttctga cagtttgaga
     2461 aaagaagatg tactccgatt ggactccaaa aaaatgtcat ctggataact aatcatcatt
     2521 ttttttgcta tgctattcgc aagttcatct ttaaaaatgt tgtaacggct aacgtcaaaa
     2581 ctaaaatgca ggctcttttc tttcttattt tcagattgtt ctctaaaata tttagcaatg
     2641 atatttaatg cttttaccgc ttgattcaaa ttgttaccaa tttgatttaa ctgtttgatt
     2701 agagctaccc tatcatcttc agccaataac ggcggttcat atttgagtcc ctttaaccgc
     2761 tcacgacaaa catctgctct agaaattcct ctttgttttg caatcaatgt cagttcaaga
     2821 taagtctttt tatcgacacg aaaatttaag gcctcactat gtttgcgatt tttttcgtac
     2881 attttttgtt tatcatccat tgattatccc ctcacatttt ttctagagag caacatttga
     2941 aattgctctc tatttcattt ttcatcatta attcttcttg aatcatcagt tcaatataat
     3001 ttgtgaccgt gattttttca tttgtcgctt tctttactga gtcgcataat tcggcaagtt
     3061 ctaagaaatc ctgcgtacta atctgtagaa ttaattgcat gacaatcacc tctttatttt
     3121 ttaaatagac tatcaaacag cggatttggt ttatcagaaa tatcctgaaa cagtggatta
     3181 ttttcataag taaatcgtgg acgtttcttc tccttcactt cttgttctac aatcactttt
     3241 tcgttaggcc tttcttttcg ttcctcgata atatttattt tttgcgtctt atctccctga
     3301 cttgcttcag ctcttgtttc acgtacttct accgttcttg gttgatttga cgcactactt
     3361 tgtacatttt ctcgtacatc tactgttcga tttccatttt gactacttgt agtattctgc
     3421 aagttttctc gtacatctac cgttctcggt tggcttgtcc cactactttg tacattttct
     3481 cgtacatata ctgttcgatt tccattttga ctacttgtag tattttgtaa gttttctcga
     3541 acatctactg agcgcctatc ttgactactt gtagaatttt gtagattttc tttgactgat
     3601 ttttctaatc cggtatttga atttttagct tcttggattc tttctttagt caacgcttct
     3661 ttttcgtcca tattagcgcc tgtacgccct tctacacgtt tccttaacat ttcctcacga
     3721 ttcattgctt caggcgtgta ttttgcttcc tgtcgcttct gattaattaa tacttcagca
     3781 gacttagctt gaccgtgcat agcattttta cgttcttcaa tatcttgttt aactttttgc
     3841 tgaattgcac ttgcaggatt aatatttgat tcagcattat ttttcattgc ttcacgcata
     3901 cgttcagaac tagacataat tctatcagcg ttttcaactt tagcgccatc ttttctatcc
     3961 gaaagacgtg ttggcataga atcagcatat tttccagttt catcttcttt gtttttagct
     4021 actataggtt tgctattttt attaatagcg tacacggtac catcatctaa accttctttg
     4081 aatttattct tggtatcatt agccatatca cgcatactct taaatggttt tgttgctctt
     4141 tcactggcca ttgtagctcc atccgaagct aagcctgata atcctcgttc acgagcatac
     4201 ccaagtgttt tccccgtttt attagctttt tgagaaagtg attgaccaaa gttgcgttca
     4261 ggttttcgtt cttccccttc cgcttgatcg tttccaccac tatttttccc acctttagat
     4321 cggttcatac cacctgttcc tttacgaaac agcatagctc ccatgccaaa tgctgaagca
     4381 agctggccat agccttcttt taaaccaata tccacgccaa agtaacgcat gactgttcca
     4441 ctacccttta tcagcacaat aacagcgcag acgtacgcta ctgcttttat aaaaggattt
     4501 acagaagaac cgctacctgt tccgagataa ttaacaaaca ttgcaaacat agacaatccg
     4561 aacccttgaa atccgactgt caaataacac tgtaaaatat ctgacaaaac ttgctttgat
     4621 cgttggcctg tttccaagtc agtagcaaat actaaaactc caagtacgcg tttaaacgct
     4681 agctcgagta ttgctgtaat tataatgaaa gcactaaaaa catacgctac cgctaaagca
     4741 atcaatccaa aagaaatccc ccatagattt gcttgatagc gataatatcc tgatttaatg
     4801 ccctcaacaa aggtcgacag aaaattatcc gaaaatttgg tagcaacaaa ttcattatca
     4861 gaattttcaa ctaattcata acgcagatta tcagctattg ggttctcttt ttccattttg
     4921 tcgagtacat tatctgtcaa tacttgcgta agatccgttt tccaaaaatt ttcttttgtt
     4981 agattgttcc ggtcattcgt tttatcgatc atggaatatt gatctgtttt attgatataa
     5041 gctaaatcag taaccccttg ttcaatcagc gaccacgcca cgccttcgtc cgaatttgta
     5101 atcgtagtcg tatcattata aaatgtttta gaaaactcta ttccgctgtg aatcaacgtt
     5161 ggcattaaaa gaatcaatga aattgacatt acaacattca ttccaacaga ttttagatca
     5221 accgttccct tacctgtaat cattttataa ccaataatca ctagtgaaag tatcattaag
     5281 ccaacaacaa tggtacttaa aacagcttga tagactttgt tcaatccggt actttctaca
     5341 aagctaaaca aatctaaaac ttcgggaatc atattttgaa tgccatcgac aacagcgaac
     5401 attgatttta taacagacca gcctatccac ataccgaact ggctgtaaaa atttccgttg
     5461 tattttttca aataattctg gtaaagcgga ttagttaaaa actcagtttg actctcagta
     5521 tcatcaaacc aattgaaaat actgccttct gcaatgttac tcgtaaaaag aatcacagct
     5581 attagaacaa gaaaaataac cggtaaccaa tttgtttttc tattagcaat ctttttttta
     5641 attattttca ttgttacctc cctcagccca ttttcttagg ctctcagaga gtttctgagc
     5701 tatttttctg tcaattatga tatgagttac gtaatcatca ttaacagctt ttaaatcgag
     5761 aacaatagta tttgagcaga tactctcggt taactcaaga gtatctgcgt tttgatcaag
     5821 acagtgaatc ttatctacta gatgattttc aatcatttta aattccctct tttttttgta
     5881 gcttgattag ttctaacgct ttgtttttta gagaagtaaa gatataatta tttttttgtt
     5941 tgatacgatg atctgttttt gctcttcgta tcaagtcacg taaagtcatt gctagctgtt
     6001 ctctaacatg atctacaaaa taactattat tacaaatttc atagataatt ttcagttgaa
     6061 ctttttgttc tttttcagca gatttaattg cataattgaa ttttttgtac cagttgtcga
     6121 attgacgata atcactattg ctagccagtt ttaagcaatt cacaatactt tctccccaca
     6181 tttgtttaag aagattttct tcaataaatg tttcgatcaa ttcttcatct aatttattat
     6241 ttttagttga aagtgattca aaaagatcat cattatgtga ttctttaata tctttactat
     6301 ctttataaga cttatataat tctagttcat tttttgaact atcagcaaaa ttgtttttgt
     6361 ctcttgtttt cattgatgtg tcgcatagtt caaattttga actatcaaca gaatattgat
     6421 ttgttccata gttcaaattt tgaactatca agtttttttg actattttct tgatgggtta
     6481 tcttattaat tttataaaca tcatctttgc acagtacagg ctttaacaaa taataaaaat
     6541 ttgggaacat ttgcatttta tttttattga agccattttt tcttttcaca agaagtttaa
     6601 ctttttgaag ttcatctagt gctgataaga ttgtattttt tgacattccc gaaatttcta
     6661 cgagattttt gatttcaaat atcaaaaaaa ttttttgatt ctcatcatac aagttatttt
     6721 tcaatgaaag tctatttcta tcttttagta acatataaac taacttagaa cttagtttca
     6781 ttttcttata tttttcattt acgtttaaaa aaataggata ttgatagtat aattctgcat
     6841 atatatcaga attagaatag ccttgctttt caactaagct ttcaacttct ttttcattca
     6901 ttgtcacacc tgcttctgat tttatttgct ataatagatt caaataaagt tttctcagaa
     6961 ctttattatt gagtgttccc ccgatctgca aactgacggg aacactcttt ttaatttttt
     7021 ctacttcatc attattcaaa aaaacacctc gttttttaaa tttgatgact caatatccat
     7081 tttttgcatt cttcttttga aaacaactcc attttttcta ataaaataac tggcaatccc
     7141 attcttttaa atttcaaaaa agttgtatat gatacattca aatatttagc catctctttt
     7201 ccttttagaa atggtctttc taacccggta tcaattcttg cttgatcaat agcctctttt
     7261 gtcatttcaa aaacataatt tttcaattga ttcgtttgtt gtttatctaa cattactgta
     7321 attcctgcat ccattatttt tctcctctca ttttctattt cttcaatcag ccaactcgcc
     7381 aagttgtatt tatttagatt tcaaatcatt aagtaaccaa tttaccaact tttgataagt
     7441 agatgctcta atactttctt tgctcccatt ttcgattttg acatatgttt tccgatttat
     7501 acctatcaat tttgctgcat cgtttttagt aagtataaga tcggctctat gtcttcttaa
     7561 gcttctaatg aattgatcat taagagttat cattcagcgc ctccttttaa attattcact
     7621 ttgtggtttt tatcttaata agcacatttt aaaccacgaa acgagtaatg tcaattaaaa
     7681 cattactcaa tttgtggttt ttatgctata atagtttgga atttactata gggggtgtta
     7741 ctttgaatag aataaaattt tatattgatt ccagtaaaaa gaccattaaa caaattagta
     7801 cagaaactgg tattaactat acaactctta cgaactatta ccgtggtgag cgaaatccta
     7861 gaaatactga aacatggaat atccttgctg attatttcca tgttcctgta agttatttga
     7921 tgggattaga cgaaacaaat tccatagaac aacctaatct tgatagaata aaaactgctc
     7981 gtttagaaaa aaaggacaaa atagtcaaga atattgccag tcttgactca gattatttta
     8041 ctgaattatc taatctatac ttttccatga cttctttact tgaagtcaaa cttcaagaag
     8101 atgactccga ctttcttttt ttaaaaagta ttctagagga ctcctttgaa tacacgattc
     8161 tatttgccaa acaaaagaag tttattaata aagctttaaa cttaaatatc gatgaaagaa
     8221 aagagttaga atctgagtta tttggcaact taacccaagt acaggttaac aatctaaaaa
     8281 gagagataac agctcgatta atatcaaaac ttcaataaaa ctaattattc aatcagccaa
     8341 ctcgccaagt tatcagattg gagagattag ttatgtcaat acaaaagtac acaacaaaaa
     8401 gcggtgcacg atatagtgtc cgcttgtatg ttcgtaaaga tgaaaatgga aagcaagaat
     8461 actatgtaaa aaaagggttt aaaactgaaa aagaagccaa gttacacgaa gcaagaaaaa
     8521 aggtcgagat tgaagatacc ggctatcata agccttctaa tgacttgttt ataacagtgt
     8581 atgaagaatg gttgccttct tataggaatc gtgtacaaga aacgacatat caacgtacaa
     8641 aagacctttt ccgcctgcat atactaccaa aattcgggaa taagtccatt tctaagatta
     8701 ctcctgttca ctgtcagaat gcagtcaatg aatgggcttt aaaattcaag aatatcaaac
     8761 agcttaagtc ttacagctct caaatatttg agtatgccat ttttgcagag ctattacacc
     8821 gaaatcctat ggcaaataca aatctaccaa agcgggaaaa gaaagaaaat gaaaattact
     8881 tttcattaga agaactaaaa gctttttggg atattctcaa taaagaagag cctttaaaac
     8941 atattttaat cttccagctt ttgattacta ctggtatacg taaaggagag ctagctgctc
     9001 ttcattggtc tgacatttat tttgaagaac agctaatgta tgttagaaag agctatgcaa
     9061 ctattcgaaa tgaagagaag aatgcaagaa gaaaaactgt caggattcaa aaaaatacaa
     9121 aaaatatatc ttccgaacga atattaccaa ttgatgcaca aactattgaa atgttaaaaa
     9181 tctggaaaaa agaacaagct cgagagcttt tgcagtttgg cataaataca aatacaaaaa
     9241 atcaacttat ctttacctat gtaaatgcag acggtgacat taatcaacct ttgcatgcgg
     9301 actattcaaa taatatattg aaacgtttag agaagaaata taaatttaag cacgttacta
     9361 ttcatggatt ccggcacact cacgctactc ttctactaga aggtggagca agtatcaaag
     9421 aaacacaaga tcgattgggt cataaaaatg ctgaaactac ccttaacact tattctcatg
     9481 ttactgaaaa agctcaacga aatgcgttag aaaaattcat tgcttacact ggtttataat
     9541 tttgtcatta cctttctcat taccagaacg aaaaaaagac accttctcgc aatgagaaag
     9601 tgtctgaaat cgttggtata acaacgatta acgttttgaa aactgtgaag ctttacgtgc
     9661 tttcttaaga cctggttttt tacgttcgac catacgtgcg tcacgagtaa gtaagccagc
     9721 acgtttaaga gctaaacgga agtcaggatc aacttctagc aatgcacgag cgataccatg
     9781 acggatagct cctgattgtc cagcgtatcc tccgccgttt acgttaacaa atacgtcata
     9841 tgcgcctttt gtttcagtaa cagcgaaagg ttgattgatt acttcacgca agtcagcatg
     9901 tggaatgtat tcttcaacat cttttttatt tacagtaatt ttgccagttc ctggtactag
     9961 acgtacgcga gcaactgcgt ttttacgacg gcctgtgccg atatattgaa cttgtgccaa
    10021 tgaaattccc tcctattaga ttaagtttgt gatgtctaaa acttctggtt gttgtgctgc
    10081 atgtggatgt tctgctccac cgtatacgtt caattttgta aattgtttgc ggcctaaagt
    10141 gtttttagga agcatacctt ttacagaagt ttcgatcaaa cgacgagaat ttttagcacg
    10201 caattcgcca gcagtgattg atttcaatcc tcctgggtag ttgctgtgac ggtaataaat
    10261 tttgtcagtc gcttttttac ctgttaattt cactttgtct gcattgatga cgatgacgaa
    10321 atcgccagta tccaaatgag gtgtgaaagt tggtttattt ttaccacgta gtacagatgc
    10381 aacaactgtt gaaagacgtc ccaatggaac atcagttgcg tctactacat accatttacg
    10441 ttctacttcg cctggtttgg ccatatatgt tgtacgcacg ttattttcct ccaatttaag
    10501 ttctgatgtt tgacatacac ttcgagtttc cggggctctt tgtggggcaa acaataccat
    10561 ttaacatatt acccgctaat ctagtcaatg tcaacgtttt tctgcttgat tgaaaaattt
    10621 atatgaaact ttacttcttt tctttctttt atataaaagg gtcgtttcct gtcaaaatga
    10681 aaaacaaaaa gaggctgtga caaaagtctt gtcacaaccc cttattttga ataaacggtg
    10741 ttctcctgcc aactcctcgg tcacaagtca caatatccat aaaacataag aagctgtttt
    10801 ttaaaattgt ttcttgtgac tcggagctga acggcaggct tacaacctct tggtaaacgg
    10861 tgttcaatca gctgaccctc ggtattagct caaaaaacgg caaaatatga ggaactattt
    10921 tgacgtttgt ttctctaata ctcaggtcaa aacgctgatt tcacaacctc tttttcggtc
    10981 aagttcaaca acagttttta cgcgtcgatc tctggcgctt tgcccaaatg agcttggatc
    11041 atccagatac gtttttctgt ggctgttttg aattcgataa agatatcttg tgttggatcg
    11101 tcgccttctt ctccagaaac ttcgattcct ttttggtaaa gatctgctag ataacgatag
    11161 cctgcaacaa gtgtttccaa acgttctgga atagtacgat cccagcggcc aatttcatca
    11221 gggatttttg tgttatctgc aaattcacgc aatgtagaat aaggtgaacc gtctaatgtg
    11281 atcaagcgtt cggatacttc atctaactga gcgttcaaat catccatgaa ctcatccatc
    11341 attgggtgca atgtaaggaa acctggtcct ctcatgtacc aatgtgtttg gtgaacaaca
    11401 acagacattt ggcttagatc tgcaactaat tgatttaata cttctttggt ttgttggaat
    11461 ttcatctttc ttgcctccta acattttctt acacttaaaa taatagtaga ctttcaatga
    11521 aaagtacaac aaaatcattt acctgagaac gattttcagt tcgctttttt atccgtaaag
    11581 ccaatcattt ttcagaatca gctaattctt ctcgcagttg agcaaattgc tgataagtct
    11641 gtgctaaatg cttttgtgtc gaaacaaata atggatagat tttctgatat tttttatgat
    11701 cagtttcttg tactccaact tgctctttca attctatcaa ttcctgtgct tcactgatac
    11761 tttggatctt tccgatgctt ctccagccta aaatcacagc tcctaatgcg gatgcttctg
    11821 tgacagtagg gaaaacaagc gggtaaccca gtacatcagc catcatctga cgaaaaatcg
    11881 gagattgagc aaacccgcct gtagcacgga tctctgtgag ctgattttcc tggcaaactc
    11941 cttttaaaat ccgataaagg ttgagacaga tcccttctag aatcgccttc atcatttctt
    12001 tttctgtatg ttgccgctgt aaaccgataa aactgccaaa cgcatctgcg tcccaaagcg
    12061 gtgctcgctc accaagtaaa tagggatgaa aaatcagacc attggcaccc gctggcacaa
    12121 aagcgatagc cgaaagcagt gcatcatatg gattttctcc tttttcttta gcagattgaa
    12181 cgacttcctg taaaaatgta tgccctgccc aatcaaagat gccagcacca ttgctagtcg
    12241 ctcctcccac tacccaatgt ttctgatcca gaacataaca aaacgtctcc atttctggat
    12301 ggaactgagg ctgagtagta ataaaacgaa gtgctccgct cgttcctacg gtcaaagcgg
    12361 cgatcgtatc accaaaagca cctaacccta gattagaaag cggcccgtct gcccctccca
    12421 aaacaaatga tatatcattt ggcaaaccta gttctgctgc tgtttttgat ggtagacctg
    12481 ttacttgata ggtggaagga aagacttgcg gcaattgctc tattcgaatg ttcaagtagt
    12541 caagcacttc ttctgcccat tggaattcat ggatatcaaa atatccagtt cctgaagctg
    12601 ttccgtagtc gctgatcagt tcgccaaaca atctataaaa gatatagtct ttgataccga
    12661 caaaataagc agtttttttg aataattcag gttggctgtt ttgcagccac ttgattttgt
    12721 tcaatggtgt cattggatga atcggcgtcc cagtctgccg ataaaattct ttgccaagca
    12781 cggtatattt cagtttttct gcttcgtccg ctgctcggtt atctgcccaa gtgatcattc
    12841 gagataacgg tgttttctct tcatccaaca atatcaggcc atgcatcgca gaggagaagg
    12901 agacaaattg aatgattgtg tctgaaggca actgtttaat tacttctttt ataacggtgc
    12961 agacaccttg aaagatcaat tcctgatctt gttctgccat cccttgtgtt tcttggatca
    13021 aagggtagcg aaaataagcg gatgctgctt cttgggcttc atctgtgaaa gcaactgctt
    13081 tggtttgtgt ggttccgata tctaatccga ttgaaacgtt cattttttca cctcaaattt
    13141 tatgtatgta caaaaattta gtttaagctt gcgtatcttg gttttgaaaa gggggagatg
    13201 gcgtgttact ttattttatc attggttgct gtatcggatc atttttatgt ttaaccgccc
    13261 agcggattcc gttaggtcac tctattattt atcctagatc tcattgtgtc aaatgctgtc
    13321 gttcgctttc ttggtatgaa ttgattccga ttctctcgat catcgtccaa cgatttcgct
    13381 gccgctattg caggtgtcgg ttgcccttct actatttgtt ggcagaagcc ttatgtggtg
    13441 gtctttttgc ctggttcttt actttttctt ccgcaagaaa tttttcgacg ttcatatgga
    13501 tgctttcggc tttgctattt tcgttgatgg atttatttta cttcatggtc cattctcaca
    13561 cgctattctg ttcttgggca attttatgga ttttttggtt gcaggccggc gtcttccaat
    13621 ggcaaagtgt gctgttaacg tttatcgtag gcggtgtgtg ccttcgctat ggtcaatcct
    13681 atctcggagc tggcgatact ctgttgatgc tttcttggag tggtggcttg tctctagaag
    13741 agttgcttca gatcttgttt cttgctagtc tgtttggaat atccgttttt tctgtttact
    13801 ttatctgtca taaaaaaaag ttggaaaagc tgcctttcat tccttttttg agcagtgcgt
    13861 tgctgattgt ccttcatctt aggtattgaa aaaaggttgt gacaaacgac ttgtcacaac
    13921 ctctgatata tagtgttctc ttaccaaatc cttgtgactt ggagctaggc gacaggttca
    13981 caacctctcc actaaatggt gttcaatcag ctgaccctca gtattagctc aaaatgctgg
    14041 ttttacaacc tctgatatac ggtgttccat aagtagcttt ttcggcttct tatttcttga
    14101 cgccccaatg tcgacgaagg aaacctgcac gtccagaacc ttctcgataa agattgtaat
    14161 ggagcttgtt tttcttgtaa taatcctgat ggtaatcttc tgcaggataa aattcgcttc
    14221 ttggttcgat ttttgtcacg attggcttat cgaatcgccc gctgtcttgc aatgcttgtt
    14281 ttgaacgctc tgcaatattt cgttcttctt catcattata gtagatcact ggacgataag
    14341 aatcaccgcg atcagcgaac tgtcctaatg cgtcagtcgg atcggtttgc tgccaataga
    14401 tttccaccaa ctgatcataa gaaataactt ctggatcata ttcgatctct actgcttctg
    14461 tatgccctgt tgtgcctgtt gttacttgtt cataagttgg atttggtaca tgcccgccag
    14521 tataaccgga tgtcacagaa atgatccctg gctgctcttc aaatggtttg accatacacc
    14581 aaaagcatcc gcctgcgaaa actgcttttt ttgtcatata aaatccccct tttttctcat
    14641 cataccttga tttccaaaag gaggataacg ttttgcttgt gacagaaaaa acggaaacta
    14701 ttatatacgt gatacggctt ctgataaatg ccaaaacgaa cgtacttgat caaaaacggt
    14761 taaatcagga atcacatatt cagcagaagg aagatttatg ttttcaccta ttcccaatac
    14821 atgcatgtta ccagccaaag ctgcttgaca accagcttct gaatcttcaa tgaccaagca
    14881 tgcatttggc ggtacattca attgctgtgc tcctttgaga aaaacttctg gatcaggttt
    14941 tgcttttgat acttgcgtgc cgtcaataag gacatcaaaa taacttgtca aattcgtttt
    15001 ttctaaaatg aggcgtgcat ttttgcttgc tgatcccaat ccgatcttga tgtttgtttt
    15061 tttcaagtac tcaagaacag ctattgcacc aggcaaaacg gatgtttcat ccattttttg
    15121 aatcgcttga agatatagat tgtttttttg ctcagcaaag gctgatttct ctttttctga
    15181 gaatttctgt tccatttttc caaatgacag caatcgctct agtgactcgg ttctggatat
    15241 tcctttcagt tgctcgtttt ctttctcagt gaatggaatg gacagctggt tagctaaaac
    15301 cagccacgct tcgtaatgat aacgagctgt gtctacaaga acgccatcta aatcgaatag
    15361 tgctgctttg attttcatcg attacctcct tgaaaatgaa cgacttgaag taaagatgcg
    15421 atgtttttag ctgtgcgatg aagattatca gtggcttgct gtaaagcttc ttgtaaaggc
    15481 acagctgcag gaaggatcgg aaaaacggcc gaaatgccaa catctgctgc tttttcgctt
    15541 cctttaccaa cacttccgca aatagctatc acaggaacgc cctctggtac tcttctagca
    15601 atcccaactg gcgcttttcc ttttagactt tgtccatcta gacgtccttc gcctacgatt
    15661 acgaggtctg cttgctgtac ccgtttatcg aactccagta aatccaatac gtagtcgatg
    15721 ccagagacta ttttccctga agcaaatgct aacagtcctg cacccattcc accaccagca
    15781 ctactgcccg atgcctcgat tacttgaggg gcaaatctca tataaaaatc atgcatggct
    15841 tggtcagcca ctgctaattc attttctttc aagccttttt gtggtccgaa aacaaaagaa
    15901 gcgccttctt ttccgcataa aagattagta acatcagtaa tgacagtgat tttggcttta
    15961 atcagctcaa taggcacgtt cgttttatca actgtagcaa ttttacctag attttctccc
    16021 agtgcttcta ctttttgatt atcttgatcg taaaaagcat aacctagacc ttctgccatg
    16081 ccgatgccgc catcgttggt actacttccg ccaacgccga tgatgatgtg tcgaacacct
    16141 tgttgcaaca attgtcgtat cagttctcct actcctcgcg tggataacct aagtggctgt
    16201 tgttggttta gtgggacatc tttcaatccg acgatttctg ccatttcaaa aatcgcagtt
    16261 tgaccgtctg aagcgtactg gatttctttg tttttgccaa atgcattagt aacgactgct
    16321 gtttgccgct tcaaccttaa actgtctgct aatgatgaaa ccgttccttc tcctccatcg
    16381 cctataggta gcagatcata ttcagcctga acaaaaactt cttgaaaacc tgattgaatg
    16441 gcttgtgctg cctctaaggc tgtcagactt tctttgaaag aatctggcgc aatcagaata
    16501 cgcattattt tcccctccgt tccaagatca gttctttttc cagctggtat tggtctccat
    16561 tgacaaaaat aggtaatgtt tctttcgtct ctttttttat tactatttgt ttttttgtca
    16621 aaacaaatag caacctgtga tttttccaaa taaaaggaaa agaaattttt tcccatgctg
    16681 aaggaagtct tggagaaata gaaagagttc cattcgttac tgtaatacct gaaaaaccaa
    16741 atataactgc tagccatagt gctcctaatg aagcagcatg aattccgtca tctgatgaat
    16801 gcggatctgg tcctaaatca atcaggcacg cttcttggaa taaatgatac gcccattcca
    16861 tttcttggat acggcaagcc tctatggcgt gaatcgcttt actcaacgat gagtcgtgga
    16921 ttgttttgtt ttcatagtaa tgcaaattct tagaaacgat tggttgagaa aaatactcag
    16981 gtaataagta taacagcatg acaagatctg cttgcttgct gacttggagt tcatttactt
    17041 cgctacgggc ataatcaagt aaaatagact ggctgccagg acgttctttg taagtggtca
    17101 aatcaataac tggcttactg agatacgtgt catcttgcgg aatcagctgt tctttgttag
    17161 gcactggcaa atataactta tctaaaaaat tttgacattc tttcaaaaaa agcggatcgc
    17221 cagcttgata tttaacagct aaggccacat tataatgcgc cagatagttc gtatatgcat
    17281 tattgtcgat atattctgtg tattcatctg gaccaatgac tctgtgtatc tctaaatgat
    17341 catcgacttt tacagctctg ctcagccaaa aagctgctgt ttcatgaatg agcgtttttc
    17401 cctttgtttc taaaaacgat tgatcctctg ttgcttggac atactcttga acggcataag
    17461 caaggtctgc actgatatga tgttctgcgg cagctgaagc aacttcttgt cttgttccgg
    17521 ttttgatatt caatgcagca aatttgggtg tctcttcctg accagtgtgt gcgctttccc
    17581 atgggaaaag tgcaccttga tagtgataat ctcttgcttt ttgccgtgcg ccttctaatc
    17641 gatcataacg gtacagcagc aaattctttg ctacttcagg aaactgatat aaaaagaaag
    17701 gcaggataaa aatttccgta tcccagaaaa catgcccttt ataaccttcg ccagttagtc
    17761 ccttcgcccc tacactaaca cttggatcat tgcggggagt cataatctgt aaatggtaag
    17821 cagcgaagtc catcgctaac tgatcgaaag aagaatcgcc ttggatcgtt atccgatgct
    17881 gatgccaaaa atctgtccaa taagacatcg actgctctaa aatttctgta taactaacgc
    17941 ttttctgggc ttgggcaata cttttcttaa acgggtctgt ttgctgaaga gaggtagaga
    18001 gcacgatgat cttctccagc gtaaaggacg tattttttct gatcttttct gaaaaggaag
    18061 cggtgatccg gcgatttttt gcttggaata cacctgcttt attgaattta gctgcaatcc
    18121 caatatcaat tttactttct gttgtctggt aggatgcact gatcaaatcg tcaccaaata
    18181 ctcgcagttc tttttcaacc aactgctgtg taccaaaatt ggtttgctgt ccatcgatac
    18241 ccgttttcac atctacttgg cagtcttcgg tcaacggctt gatgaccact ttcagggctg
    18301 cctgatggag attattttga tcagcaacac gacaagtaat gacttccact ttttttcctt
    18361 gagcagtttc aatgagcgca tagcgtttaa gctctcccat ttctaaatct agtgcccgct
    18421 cgtatgcaag aactttcatc gtctcaagag aaaaatgttc accgtcgatt tttatttccc
    18481 atctgatgac atctgggaga ttcactaact cggctgattc tgctcctaaa ggtcggttat
    18541 atacaccagc tgcaaacatt ccccttactt gattccgata gtcttcctcc tggcttcctc
    18601 tgataccaaa aaatccattt ccaatggtca tctgactcgc atacttattg atatactcta
    18661 aatcaaactg tttttcttct actttcataa ggtaataact tctcctgttt ctgccgaacg
    18721 atataaattt tccaccagat tctggatgac atatccttgc cagccatctg caatcatctg
    18781 agtctggtca ccgagcgccc gattcacaaa atgagccata cttagttcat gtgggtcatt
    18841 aactggcgtg gtcttttgta aggaaaccag ctttccatct acatcttcga atagttcgcc
    18901 tggatacaga ttagcacctg cctgatcgcc acaaaaagcc acattcagtt tgtcttctct
    18961 gatattcaaa gcaaacgaag tattcagttg gatgatcttg ccgttttcta attccagtgt
    19021 gccaaaaatt gcatcttcta ctgtgaattt gcttggatcc catttcccga atttccctga
    19081 attttttttc gtaccaattt tttggaagct gtacgcccga acgcttttta ctttaggaaa
    19141 tccagtaata tacatcgctg tatcaagcat gtgaatgcca taatcgatca aaggcccgcc
    19201 tccttgaagt tctttgtcga taaacgttcc ccagcctgga acaccagacc gacgaagcgc
    19261 attcgcttct acgtaataaa tatccccgag aacatcaaga tgattcttga tcattgttgc
    19321 ttcgtcagaa aaacgatgct ggaatccata agccaaaatc tgcccgtttt tatcggctgt
    19381 ctctgccatc tttcttgctt gatcagctgt catagctggt ggtttctcgc acatcactga
    19441 agcattagct tcgagggcag ccaatacatg ttcgaagtga aagcgattgg gtgtacagac
    19501 actcacgata tcgggagaaa tctcattcag catttgtgta actgatgtat acgcatgaga
    19561 aaatcctgta cgtcggcaaa agtcttctgc tcgctggctg actggatcaa ccacagcggc
    19621 caattctaag acatctgatc ttttttgata ataagcagca tgtacttttt ctgctacttg
    19681 tccagcgcct atgattgcta ctttcttcat ttatttctcc ctcctttgct tatttactta
    19741 ttgttattct ttctctattc tttctctaaa tcatttaaat gacgcgctgt ttccagacag
    19801 tttttcaagt attgcaatga ttcctgataa attttcactt cattttctcc tcgcaaacga
    19861 cattcatata ccatataacc ttcatactca ttttgaagta gcgtttgaaa atggcgagta
    19921 aaatcaattg cgccagatcc tggttggtag cgatgattat ctgctaaatg gatatgaccc
    19981 acatactctt tttgcgtagt caatgctttt gaaatatcat cttcttcaat attcatatga
    20041 taaaaatcag cgatgatctg cacatgtttc aattgattag agtcaatgta atgacgcgca
    20101 tccgctaaca aattgatcat atggtcttgg tatcggttca aaggctccaa ataaataacc
    20161 gtacctgttt cttcggctac tttatctaaa taacgaagtg attcgctgac tgctttatag
    20221 tctccttctt tgcttcgggg tgaaaccatc ggcggtaatc gataagtgaa cattccccaa
    20281 gctgctggga taacgattcc tttgcctcca acctcgctta gtgcacgcaa aatctcagca
    20341 atttgacgta caccattaag acgccgttct tcgataaagt caccgatcca cccatcgtag
    20401 ccgttacaag ctgtcactac tgggatccct gtttctttga ttgcctgttt gactgtgtcg
    20461 atattttcta ttaacaactt accgtcgatt tcgtattctt caaatccaca gtctttgata
    20521 aatctaaatt tttctaaaat gtctcttggg aaaaatgctt gatcttgcgt accgaatttc
    20581 attgttctcc tcctcggact tgaacgccca ttttgatact ttcttctggg tgctggtcta
    20641 cataacgcat gtaagcttct gcgctttctg caaatggcac aactggatca atgatttctt
    20701 cacaatccaa gtaaccgttc atcaataatt cccaacatgt gtcctcgatt cgtttacggt
    20761 tccatcttgg gtaatctggg tttggttcac tagatgctcg agcaaagacg atttttgcat
    20821 tattgaaatg tgcttcgcga ccaagattaa acccagctgg aaaaggttta gcaaaggcaa
    20881 cataagaaat gatcccgcca tagccgatcc ctcttaaaga agcttgtaat gcggcagcac
    20941 tgccactcgt ttcaataact gaatctactc cacgtttatc tgtcaattcc tttagacgaa
    21001 aaccgacatc tgctttcata ggatctagcg tgtaatcggc accgtatttt tctgctaatt
    21061 ctctacgctg cgctatggga tcgatcccaa cgatggtagt agcacctgct tttttagcta
    21121 attgaatcgc aatttgcccg atagcgccta aaccgaagat tgctacagca tcacctgctc
    21181 gaacatgtgc ttcccggata ccacctaaag cgaattgagc aggatcgtaa catactgcat
    21241 ttttccattg atccaaccga tcaagtttac gtagtttata attatcaaca ccgtttgtaa
    21301 tcagcgtttc acgaatgggt ccataagtgc agactaactc tcctttttga tactctgtca
    21361 ccaaactgcc cgtttcttcg atcgtaccga caatcatatt tcctaattga aattcgccaa
    21421 ataccacacc gcgttggctt ccttcttctc ttggcaagaa taggcgccat tcttctgaaa
    21481 attcttcatc gataaatgga cttaatcctc taaagtcgac aatttctgtt cctggttttg
    21541 gagtagcgaa aattactcgg atacgtactt cgttcttttc gatctcacgg tcttcgtaat
    21601 ctttgaatcc ggctattctt ggttctaatg caactaattt tttcatcttt tctttcctct
    21661 ttcttttctt tttatccttt tacgccgcct tcgactaaat cacctttaat aaatcgttca
    21721 gataacgcat acataatgat gactggtaaa gcggtcacca atgaagcggc catcattcgt
    21781 ccccaaatgt agtctggtgt actgaataat gtgttcaaac cgattggtaa agtaaatttt
    21841 tcggaggtcg acaggaaaat cgaagcaaat aaataatcat tccacgaaac cataaagcaa
    21901 taaacgaaga cagaaatgat tccagaaata gccaatggta taatgatccg aataatgatc
    21961 tggatccggc taagaccatc gatcattgct gcttcttcaa tatcagtcgg aatcgtgtca
    22021 aaataacttt ttagcatgaa tatcgcagta ggaagtgttt gaacgaccat cgtgataatc
    22081 agtgctgtat tggtatcata caaacctaga ccggaaatga ttttaaataa tggaacaacg
    22141 atcaaaatac cggaaaacat ataaacggtg taaaagctag cattgatttt cgagcgagct
    22201 ttgaagcgta gttttgacaa agcgtatgcc ccgctgatcc caatcactac tgcagttacg
    22261 gaggctatca aggagatcac taaactattt ttgaaataca tgatgaatgg aaaaatctca
    22321 gcattgaaaa tatcctgata atgttcaaaa gtccattctt tcggaaaaat agtttgtgtc
    22381 gttgcaattg cttcttgtga atccttgaat gatgtcatca gcatgatcaa aaatggaaac
    22441 aaattgacag ctaatagcaa caaaatcgtc gcgtaaaata agattttcct gctcttttta
    22501 tttttcattt caagtccacc ttctttcgtg agagcaagac gaccgctgca ataatgatga
    22561 acaatagaat cgatatagct gctgcttttc caagatcatt gaacgcaaag gctgtctgat
    22621 acaaataaac gcctaagata ttgactttat tcgtcagtag atagacatct gtaaacatat
    22681 agaacatcca gatagatcgt aaaacaatca cagttgctag tactggtttg attgccggca
    22741 gtgtcacgat cttgaacttc tgccaagcat ttgcaccgtc catttcagct gcttcataaa
    22801 gcgttttgtc gactgtttgc aaaatagcca aaaatgaaat aaacgcatac ggatagtatc
    22861 tccagatagc aaacaaaacg accataaaga agctagcaac tggctggtcg aaccacagcg
    22921 gtgcttcgtt gaaaagctgc aacctatcta ctaacagatc attgacgatt ccataactat
    22981 tattgaacat atatttccaa gcaaaaatca aagaaataga tggcgtatca taacttaata
    23041 aaatcagtga acgcaccgtt ttcctaaatc tgaacttgcg gttaagcaaa atagctacgg
    23101 ctaatcctaa agctgtactg cctgctacaa ctaagaatgt gtaagcaact gttaaaccta
    23161 aagaacgata aaattcacta tcacttaaaa tttcaagata attgcttaat ccgataaaaa
    23221 cggcgtctga gttaggattc agcggcatat cgaaaaaact gatctgaata ttggaaagca
    23281 ttggaatcag cactaaaaca gctaataaaa gcatgcttgg tgctaaaagt cccatggcta
    23341 attttaagtc agaatgtttc cttggacgat tcattgaaac ctccccctga taatctgcca
    23401 atagctatct acttttttcg aacaccattg gcagaacttc ttgttatttc gtattttttt
    23461 gtgcgtcagc taaagcttga tcgacatctt cgtttccgac agttacttgg ttcactgctt
    23521 ttccgatcac acctgagctt gtgatatctc ccattttcgt gaaattcttt tccccgacta
    23581 gtccaaagac ttgtacttca tcaaaagcac tagcaatctc agatggcagc tcgccaaaag
    23641 acttgatgac ttcattttct ttgtaaccat ccagttcgac tacttgttta ttcactggtt
    23701 gagcaccacc aggagacatc aagacccact tttccatgtt tttcggttga gataagaaag
    23761 ctgtgaatgc ttcagcagcc tctttttgtt tctcgtctag accagaagaa atcgtcaatc
    23821 cagatactgt gccatagaca gcttgatctt tttgtgttgg gatagcaaaa ccgatatttt
    23881 ctgagttccc cgcttcataa acagaaggca aaatgtaagt tgaatagatt gccattggtg
    23941 ctgttccatt catgaaagca tctttgattt ctgtcacatc attagagcct ggcatcgtgt
    24001 attgggataa gttttgataa aacgacaatg cttctctcat cttttccgta tcgatcgtta
    24061 cctcgccttc atcatccaaa acatttgctc catttgacaa agcaaattga gagaacgctt
    24121 gctcactcat tgttccttca gcagtaggca tagcaattcc atattgttta ttctctttat
    24181 ctgtgaaatg ttgagcaatt tttaaaatat catcccaatt ttctggttct gaaaaaccag
    24241 cttctgataa tttttctttg tcataccaaa taccttgaac ccagccgcta attggtgcag
    24301 caatatagct ttttccatct tctgaacgaa caaggttttt ggctccgtca taataattat
    24361 cttctccagc ttcctcgata acgttttgga ctgccgtctg atcaatcagt tcatctttat
    24421 ccatcacttt agcaaaatcc tgactgactt ccattacttc tggtaattta ccggaacgag
    24481 ctaacgtaac gatttttgta ttgaacgctg attcttctac cggcacttgc ttgattttga
    24541 tgtctggatt ttctttttca aaatcagcaa ccagttcatt gatcacactt aatcgttcct
    24601 gttcgacaga agagtgcatg aattgaatcg ttgtctgttg tgattcgtta gagctttgac
    24661 tttcaccaca agcagtaaga agaaatccag tagctagcag acaggtagta acaataattt
    24721 ttttcatttt tttccacctc ttattttatt attttcagcc acatatattc atacggctgg
    24781 aatatcaact ttcgttcttg actgcaatgg ataatttgtc cagaaaacag gtctaggtaa
    24841 tctccttttg gtaaaagata acctgctgat tgattggaaa aattatggat aactaagact
    24901 ttctcttcct tcgcttcgcg aataaaactg ataaaagaat ctgctgagtc cagtaagtaa
    24961 atcaaactat tcggatggaa ggctttttct ttttttcgaa tagatagcat ctgggtcaat
    25021 tcaccatata ctgctgttct taatggatct gtctgcaact ctttctcaat atctgctaac
    25081 aaaaattttt gccgattgat ttttcgattc tctcctgttt tttctacgcc tttgtaatca
    25141 tttcgacttc ccagcacact ttgaatataa attgccggca cgcctggtaa cgttaccaaa
    25201 aatgaatgag cagctaaaaa gcgtttgctt ctccattccg tcgtatctgt tggagaactt
    25261 aacgcatcta gatacgtagc gtttatctca tatggactgg tcgttccatc tgggtttttc
    25321 ttgtagttta ctcgggcacc ctctttcttc agctgttcca ctaattcaag gatcgttttt
    25381 tccggcagaa tacctcgtac cggattcaat ccgataccgt catgggaggc taaaaaatta
    25441 aaaaaagttg tttttttcga tgcgaaatcg atggttttta accattcttt caacttactt
    25501 gtattacccg aacgaatcgc ttgaagggtc aaaggtggta gcgaaaactg atagaccatc
    25561 tgtgcttcat tctcgccatt cccaaaataa ctgatattat ctttgtgtgg cacatttgtc
    25621 tcagtgatca ataccgttcc tggagctgct tcatcagcga ttgcacggaa taattggatg
    25681 attttatgcg tttctggcag gtgtatacaa gaagttccta gttctttcca taaaaatccc
    25741 acagcgtcca agcgaataaa tgaagcgcct ttttctaagt aaaacaaaag aatatcgatc
    25801 attttcaata aaacttctgg atttttaaaa ttcaagtcta cttgatcttc gctaaaagtc
    25861 gtccatacgt gtttttcttt cccgtttttg tcaatgaacg ttgtaagtaa cgggctggtc
    25921 cttggtctag tcacgctgct tgtatctgta tccagtggca aatcaataaa aaaatctttg
    25981 tacgtatcat tcaagtttaa atattcctga aaccaagtac tttcggcaga catatgattg
    26041 cagacaaagt cgaacattaa ccgtgtttcg cttctcattt cttctatatc ttcccatgtg
    26101 cccaaacggt cgttgattgc ctgataattt acaacggaga aaccatcatc agatgtatat
    26161 gggaaaaatg gcaagaaatg tacaactcta aatgtatctg ccaaatactt ttgataaaat
    26221 ttcttaaatg tacgcaaagt tggttgctgc tcttcaaaaa attgatcccc ataagtaatc
    26281 aagaaaatat cttcttcgtc ccaatgcagt ttgcctgcct ggcctttctt ctgctgataa
    26341 acactgactt tttgttcaat catagaggcg gttttctcag acaaatctga gtcgcgataa
    26401 atcgcttgta aaagttttgt atacatcgga ttcccctttc ttttttggga gcgttcccaa
    26461 cgatttgatc atattatgga aaacgcttac tgtcaatagc atttttttaa ataattttga
    26521 tatacacaaa aatacaaata agaaagctta tgtatcaata attaatagct tcttaaaaaa
    26581 atatggtctc tcatgatcat gcaagctatt cctaaatttt caaaatcata gacaaaaaag
    26641 aatgtctatt gtactatgga attattctaa aacgctccca aaaaggaggt gcatataaaa
    26701 ttgaaaccta ctatttatga cattgcgaaa aaagctaatg tctcaaaatc aactgtctcc
    26761 agagtattaa ataaccagcc aaatatctct gataaagcaa aagcaagtgt acttcaagca
    26821 atcaaagaat tgaattatga accgagcaaa attgcaagag gactgtcagg aaatggtttt
    26881 gacgcgattc tagcattatc tcatcgtact tctcatacga cctatggcaa tccttttttt
    26941 tcagatgtta ttcaatccat ttctgccgtt tctgaacaaa ataacttcga cttgattcta
    27001 caaacggcaa aaacaccaga agaagagcta gaaaaatgcg cgaaaaagat ccaagaaaaa
    27061 atgatccgag gaattattat tttaagctct acgatggatg aaacacttct ttttgaattg
    27121 gatcaataca atattcctat tgtggtaatc ggaaaagtcg gaaacgacta cacgaacatc
    27181 tactcagtgg acacggataa tttccaggat agttacgatt tggtggaaac actgatcaag
    27241 aatggacacc gtcatatcgg ctgtctccat gctccgacag aagtttatgt atccaatgat
    27301 cgtctcaatg gttaccgtca atgcttattt gatcaccacc tagatatccg taatgaatgg
    27361 attatagacg gcggctttcg cttagaagat agcatggcag caatgaatga tttaatgaag
    27421 ctacgagaaa gaccaactgc gattttttct accgatgcat taaaatcatt gagtatttac
    27481 aaagtggcag aacaatctgg aaagcagata cctgatgatc tttcgatcgc aggattcagt
    27541 gatcccttat tgtcctccct attttcgcca cagttgactc ggatagacat tccgacaaat
    27601 cagttaggaa tcaaagccac agaactttta ttccaactaa tccaccacca ataccctgaa
    27661 gaaaaaagaa tcattctttc aaccgagatt actctcacag attcgatcca acaacaagaa
    27721 atgtagaaca gaattataaa agaacagaaa cactgaggat aagtcagtta ctctgttctt
    27781 ttttcaatca ctcatccctc gttaccccct cccggtattt tagcgaattt ttcttatttt
    27841 ccatctgttt tagatagctg tggataacta taatgtttgt gttaaactat tttttccaca
    27901 gggaagaaaa aactattttt aaccacgttg tgtataagtt tttatagttc gtttttttaa
    27961 acacatttac ccttatttca atccatgtta tttggggata cttatctcta aaacataaat
    28021 agaataacag acatgtctac tggttttgtt ttattcgagg aattattcaa taatagctag
    28081 caaaatatta aaaataaccg ctctatcttc ccatcaattt attttgcatt caaaaaatga
    28141 tctatcttta cttttgttga ttgacttctc ttggaagatg atcgttccga taccttaatt
    28201 ggatacgaac cgatgttgtt aagatccttt cccacttttc ttctagttag acaaattatt
    28261 ttagacaaac caaaaaagag gttgtgacaa taatttgtca caacctcttt tttaaaatat
    28321 ctattttgtt tgattgttca ttgcgtcttc tgcagccatt tgcgcttcaa tatctgaaat
    28381 actgtagaca ttttcgcttg ctacaccgac ttcttttggt tccatgtcac gataacgttg
    28441 cataccagta ccagctggga tgatcttacc tataataaca ttttctttca aaccaagaag
    28501 aggatctttc ttaccgcgga tcgcagcatc cgtcaagaca cgagttgttt cttggaatga
    28561 tgcagcagat aagaaactat ttgtttctaa tgatgctttt gtgataccta gtaagacagg
    28621 tcgagaagtt gcaggaacgc ctcctgagac tagcgtattg tagttttgat ctttaaagtc
    28681 tgcgatgtct aataatgtac ctggtaagat atctgtatca cctggatcca ttacacgaac
    28741 tttacgcagc atttgacgaa ccattacttc gatatgcttg tcgccgattt ctaccccttg
    28801 catacggtaa acacgttgta cttcacgaag caagtagttt tcaacagata atacatcgcg
    28861 aacttgcaac aattgttttg gatcgattga tccttctgtc aatggtgcac cacggtggat
    28921 catatcgcct tctgctactt tcatacgtgc tgtataagga acagtatacg tacgtgtatc
    28981 tgtttttcct ttgattgtta cttctttggt acgtgttgca ggatcttctg agatatcgat
    29041 gacatcccct gttacttctg tgatcactgc ttgccctttc ggattacgtg cttcaaagat
    29101 ttcttggaca cgaggaagac cttgagtgat atcgtctccg gcaaccccac ctgtatggaa
    29161 tgtacgcatc gtcaactgag taccaggttc accgattgat tgagcggcga tcgtaccaac
    29221 tgcttctccg acttcaacgt cagagccagt tgcaaggttg cgtccgtaac aatgtttgca
    29281 gacaccatgt tttgtattac atgtgaagac agaacggatt gtcactgttt cgacacctgc
    29341 atcaacgatt tgttttgcga tgtcttcgga aatcaattgg tttcctgcaa tgatcaattc
    29401 atttgtttca ggatgacgta cttctttacg ggtgtaacgt cccactaaac gatcttctaa
    29461 tgattcgatg atttcatttc cctcgcggat ggcttggatt tcaagacctc ggtcagtacc
    29521 gcaatcttct tcacggatga ttacatcttg ggccacgtca accagacgac gagtcaagta
    29581 acctgaatcg gcagtcttca aggctgtatc ggtcatccct ttacgagccc cgtgagttga
    29641 gataaacatt tccaagacgg atagtccttc acggaagttc gaaatgattg gcaattccat
    29701 gattcgacca ttaggagcgg ccatcaaacc acgcattccg gcaagctgtg taaagttgga
    29761 aatgttacca cgagctccag aatcagacat catgaagatt ggatttttcg cttccaaacc
    29821 ttccatcagt ttttgttgga tgctgtcttt tgcagcattc caaacagcaa tgacacgttc
    29881 ataacgttcg tcatctgtga tcagaccacg acggaattgt tttgtaatag tttcaacttg
    29941 tttatgggca ttttcaatga tttcttgttt ttcattcagt acactgatgt cagcgatccc
    30001 tactgtgata ccggcatgtg tcgaatgttt gtaccctaag tctttcatac ggtcaagcat
    30061 cttagaagtt tctgttactt tgaatcgttt gaagacttct gcgatgatgt ttcctaagtt
    30121 tttcttcttg aacggtaata ctaattcttg ctctttgata tgtgcaggaa tatcagtacc
    30181 agcttcaaca aagtatttgt ccggtgtttg gacggtcaag ttgaagtctg ttggttcatt
    30241 cagataaggg aattcaggag gcatgatctc gttgaagatg atcttaccaa ctgttgtgat
    30301 catcgtacgt tctttttgcc attcagtaaa tggtttttca cctaatgagt tagggttcac
    30361 accgatacgt gaatgtaaat gaacatatcc gttgcgccat gcgataacgg cttcgttttg
    30421 atctctgaag accattcctt cgccttcgcg tccttcttct tccattgtca agtagtagtt
    30481 acctagaacc atatcttgag aaggagtaac aactgattta ccatctttag ggttcaagat
    30541 gttttgagca gctaacatca acatacgtgc ttctgcttgt gcttcttcat tcaatggaac
    30601 gtggacagcc atttggtctc catcgaaatc ggcattataa gcttcacata caagtggatg
    30661 caaacggatt gcgcgtcctt gtactaaaac tggttcgaat gcttggatac caagtcggtg
    30721 aagcgtaggt gcccggttca gcaatactgg gtgttccttg attacgtctt ctaaaacatc
    30781 ccatacttcg tcttcttgac gttcgatttt acgtttcgca tttttgatgt ttgaagctag
    30841 ttcgcgttga acaagctcac gcatcacgaa tggtttgaat aattcaatgg ccatttcttt
    30901 tggaagtcca cattggtaca tcttcaagaa aggtccaaca acgataacgg aacgacctga
    30961 gtagtcgaca cgtttaccta gcaagttttg acggaaacga ccttgtttcc ctttcaacat
    31021 atgagaaaga gatttcaatg ggcggttacc tggtccagta actggacgtc cgcgacgacc
    31081 gttatcgatc aatgcatcta ctgcttcttg cagcatacgt ttttcatttt gaacgatgat
    31141 tccaggtgca ttcaagtcta acaaacgttt caaacggtta ttacggttga ttacacgacg
    31201 gtaaagatca ttcaagtcac ttgttgcaaa acgtccacct tctaattgaa ccattgggcg
    31261 taaatctggt ggaataacag ggataacgtc cataaccatc cagcttggtt gatttcctga
    31321 agcgcggaat gcttctaaaa tatccaaacg gcggattgca cgtgtacgtt tttgtccttg
    31381 tgcagatttc aattcttctt tcaattcggc tgcttctgca tctagatcaa cattatcaag
    31441 taattgttta atcgcttctg cacccatagc agcttggaat tcttgtccat attgttcacg
    31501 tttttcgcga tattcgcgtt ctgtcaacaa ttgttttttc tctaaattcg tatttcctgg
    31561 ttcgatgact acatatgaag caaaataaat gatttcttcc aaagcgcgtg ggctcatgtc
    31621 taatacaaga cccatacgtg aaggaatccc tttaaagtac cagatatgtg aaacaggagc
    31681 tgctaattca atatgtccca tgcgttcacg gcgaactttc gaacgagtaa cttccactcc
    31741 gcatcgatca caaacgattc ctttataacg gatacgtttg tattttccgc aggcacattc
    31801 ccagtctttt gtaggaccaa aaatccgctc acagaacaac ccttcgcgtt caggttttaa
    31861 ggtacgatag ttaatcgtct caggtttttt tacttcaccg taagaccagc ttctgatttt
    31921 ttcgggagaa gccaaaccta tttgcatact ttcgaattta tttacatcga tcaaaagggg
    31981 ttccctccat ttcatttaat ggcaaaccgc aagacggctg atcttcgacc gccttgcagc
    32041 tcatagaccg gattagtcta aattatcttc tgcggtttca aagttttgga tgacatcgtc
    32101 tttttcatct tccacttgtt cagcagcttt cttttctaat tctttggcag tttgctgttc
    32161 ggcaaatttt gtcaaagcat caacagtgat caaatcgtcg tcttcatcgt ccatgtcgcg
    32221 aagttcgatc tctgtttctt cgatatccaa gacacgcatg tccagaccta gtgattgcaa
    32281 ttctttcacc aatacgcgga atgattccgg tacacctggt tttggaattg gttcaccctt
    32341 gacgattgct tcgtatgttt tcacacgtcc gactacgtca tctgatttgt atgtcaagat
    32401 ttcttgtaaa gtataagccg caccgtaagc ttccagtgcc catacttcca tttcaccaaa
    32461 acgttgtcca ccaaattgag ctttacctcc caatggttgt tgtgtaacca atgagtaagg
    32521 tccgattgaa cgtgcatgca atttgtcatc gaccatgtga gcaagtttga tcatgtacat
    32581 cacaccgaca gacacacgtc catcaaatgg ttcacctgta cgtccgtcgt acagaacagt
    32641 tttcgcatcg ctggccatac cggcttcacg aactgtttcc catacgtctt catcacttgc
    32701 accatcaaat actggcgttg ctacgtggat acctaactga cgtgcagcca tacccaagtg
    32761 taattccaat acttgcccga tgttcatacg tgaaggcacc cctaaaggat tcagcatgat
    32821 atcgattggt gttccatcag gtaggaatgg catatcttct tccggcataa tacgggatac
    32881 aaccccttta tttccgtgac gaccggccat tttatcccct tcatggattt tacgtttttg
    32941 aacgatataa acacgaacta acatgtttac gcctggtgac aattcatctc cagcttcacg
    33001 agtaaagatt ttcacatcgt gaacgatacc gccgccgcca tgtggtacac gtaatgatgt
    33061 atcgcggact tcacgagctt tttcgccaaa gatcgcatgc aatagacgtt cttctgcaga
    33121 taattctgtt accccttttg gcgtgacttt ccctacaagc aaatcgccat cttggacttc
    33181 tgcaccaata cggatgatac ccatttcgtc taaatctttt aatgcgtctt ccccgacatt
    33241 agggatttcg cgtgtgattt cttcaggtcc taattttgta tcgcgggctt ctgattcata
    33301 ttcttcaata tggatagaag tgtatacgtc atcttttacc aaacgacggc tcatgatgat
    33361 cgcatcttca tagttgtacc cttcccaagt catgaaacca actaaaacgt tttggcctag
    33421 agccatttcg ccttgttcca tagatggtcc gtctgccaga gtatcccctt tttcgacttt
    33481 ctcacccaaa tgaacgattg gacgttggtt gtagcttgtt ccggagtttg aacgacggaa
    33541 ttttgttaca tcgtatttat ctaatgcacc gttgtcgcgg cgtacgcgga tttcagaagc
    33601 atcaacgtat tcaacgacac cgtcatgttt acataacaaa gcagctcctg agtcatgcgc
    33661 tgatttgtat tccataccag taccaaccca tggtgcttga ggattgatca acggcactgc
    33721 ttgacgctgc atgttggcac ccatcaaggc acggttggag tcatcgtttt ccaagaaagg
    33781 aatacatgct gtcgcaacgg caactacttg tttaggtgat acgtccatgt agtctacttt
    33841 atcaatagat acttctaagt tttcactttg agcacgagcc ataacaacgt cttgagcaaa
    33901 tgttccatct tcattcaatg ggctgtttgc ttgggctacg atgtaatggt cttcgatatc
    33961 agctgtcaag tagtcgattt ggtcagtaac acgacctgtt tcacggtcta cacgacgata
    34021 aggtgtttcg ataaatccga atttatttac tttcgcataa ctggacaaac tattgatcaa
    34081 cccgatgttt gggccttcag gagtttcgat cggacacata cggccatagt gggagtagtg
    34141 aacgtcacga acttcataac cggcgcggtc acgagtcaat ccgccaggtc ctaaggctga
    34201 aagacgacgt ttgtgtgtca attcgccaag agggtttgtt tgatccatga actgtgacaa
    34261 ctgagaagaa ccaaagaatt ctttgatact tgccacaact ggacggatat tgatcaattg
    34321 ttgaggtgtc aatgtttcag tatcttggat cgacattctt tcacgaacca cacgttccat
    34381 acgtgctagc ccgatacgga attggttttg taaaagttca ccgactgaac ggatacgacg
    34441 atttcctaag tggtcgatat catcgacatt gccgatacct tccatcaagt tcaagaaata
    34501 gctcattgaa gcaatgatgt cggctggacg aaccgttttt acagctgctt ctggatagcc
    34561 gtttccgatc acgtttactt cacgttcagg gtctcttggt gaaaatactt tgatgacttg
    34621 gacagtcatt ggatctgtga ccacaccgtc ttctgatgga tagtaagtca ctgaatttag
    34681 gccattatca atgaaaggag ctagtgtttc catgacttgg tgagtcagaa ctgttccttt
    34741 ttcaacgatg atctcgcctg tttctggatc aactaatgtt tcagctaatg tcagattcaa
    34801 tagacgtgtt ttcaaatcta attttttgtt tactttgtaa cgaccaacgt ttgctaagtc
    34861 gtaacgtttt ggatcgaaga aacgagcatt cagcaagtta cgtgagctgt ctgctgtttt
    34921 tggttcgcct ggacgcaaac gttcgtaaac gtctttcaaa ccttcttctg ttcttgagtc
    34981 gcttgcgttt ttatgcaagt ctttttcgac tgtattgcgt aatgtttcgc tgtcgccgaa
    35041 gatttcaaag atcgtatcat cggaaccaaa tcctaacgca cgaactaata cggttaaagg
    35101 aatcttacgt gtacggtcga tacgtacata agagatgtct tttgcatcag tttccatttc
    35161 tagccaagct ccacggttag ggataacagt agaaccgaat ccttcttttc cgtttttgtc
    35221 gacttttcca tggaaataga cacctggtga acggactaac tgagaaacga ttactcgctc
    35281 tgccccgttg atgatgaatg ttccttgttc tgtcattaat gggaaatcac cgaagaatac
    35341 ttcttgtgct ttgatttcgc cagtctcgcg gtttgtcaaa cgcaatgtta catgtaatgg
    35401 tgcagagtag tttgcatcat gcgcacgagc ttctgctact gtatactttg gttctttcaa
    35461 ttcataatct acaaattcca aagatagatt gccattgaag tcatcgattg gcaaaatgtc
    35521 ttcaaacatt tctctaagtc cctcatctaa gaaccattgg taagagtctg tttgaatttc
    35581 gatcaaattt ggtaattcca acacttcact gattcttgcg aaacttctac gttcgcgatg
    35641 ttttccgtat tttactacgt gtccagccaa gctcttcacc cctctaaaat gtcattcaaa
    35701 aagactaccc gatcaattgt tacaataata tttcaaatat tatttttagg taacaacaga
    35761 cattttttgc ggcttttagg caaaaaaaaa ccaaaaatag aacattctct tgaaacctta
    35821 cttcgtgagc ttctactttt gtttccttat attaaagccg aaacggacaa tatttcgttt
    35881 ctgccttttc ttcatcagat agtttttgca acttcatata ctacacttat tttctataaa
    35941 agtcaacact tcctggcgtt tcttctgctt tttcgcaaca ttttcttaat gttttcaatt
    36001 ttatgttttc aattatctga caattcttaa ttgacagacc tttttatctc ggttatactt
    36061 tagaaaattc attttgtgga ggaacttcta tgcgaaccaa catcaaccaa atcgcaccaa
    36121 cacaactgaa taacttttac tttagctact ttagataggt ttgtattcat catgatggat
    36181 acaaacgtta aacgacagtt tgtatctatg atggctaaag aactttgtat tgcagcaaaa
    36241 agccacgtag acaaaatgct aagtggctga gcagtgaaaa tacaaagtaa aagtatcaag
    36301 cattctactt tttcaattta ttttccgact tttcaatcca tttggacatt ttgtctgaat
    36361 ggattttttg tttcccaaaa ttaaggagga aaaagaaatg aaaaaaaata taatcatcgg
    36421 attaggcgct gccctgctac tagctttagg aggttgcgca caaaaagaca attccgcaga
    36481 ggcatcagat ggacaaaaaa caattggcgt attgcaactt gtacaacata actcgttaga
    36541 tgctgcctat aaaggattca aagaaggatt agcagaagaa gggtataaag aaggcgataa
    36601 tctaacaatc gattatcaaa atgcccaaaa caatcaagac aacctgaaaa gtatgagtga
    36661 gaaactcgtc aaagaagatc ctgatttgct acttggaatc gccactccag cagcacaatc
    36721 tttattaaat gaaacaacca ctatcccgat gactgtcaca gcggtcacag acttggaaca
    36781 agcccattta gtcacatcca atgaaaaacc tggcggcaat gtgactggca cgacagatat
    36841 ggtgccaatc gaaaaacaaa tcggcttact gttaaatatc gtgccagacg ccaaaacaat
    36901 cggcatcatg tataacaacg gtgaagcaaa ctctaagatc caagctgatt tggcagaaaa
    36961 agaattgaaa aaagcgggcg tcaaagtcaa agtactgaca gcaaattcca caaatgatgt
    37021 ccaacaagtg acgaaaagtc tagctaaaga tgtagatgga atctatattc ctactgataa
    37081 cacgttcgca agtgcggcaa gtgttgtcgg tgaagtcgca aaagaaacaa aaacacctgt
    37141 cgttgctggc gcagttgatc aagtcaaaga cggcggtcta gcaacagtag gaatcgacta
    37201 cgaaagcttg ggcaaacaaa cagggatcat ggcagcaaaa atcttagacg gcaagaatcc
    37261 aagtgaactt cctgtcgaaa cagcgaatga tttggaactg tatgtcaata aagacatggc
    37321 caaagcatta ggcatcaatc cagacagcat caaagaagct gaataaaaca caggaggatt
    37381 ctcatgacaa atttgctctc tcttttctta tcatctgcat cgcaaggggt cttatgggcc
    37441 ctacttgcga ttggtgtata cgtcactttc cgtatcttgg acattgccga cttgactgct
    37501 gaaggaagct ttcctttagg tgcagggatc gcggcagtaa gtattacaaa tggctatcat
    37561 ccattgacag cttgtcttct aggcttttta gggggtgcgg cagcagggct tgtttccgga
    37621 ctgcttcaca caaaattgaa aattcccgcc cttcttgcag ggatcatcac catgactggt
    37681 ttatattctg tcacatcccg tgtgatgggt gcgccgaaca tctctttatt gggtcagcca
    37741 acgatctttc attgggtcga gtcattcggc ataacaaaag acaacgctgt actgatcgtc
    37801 ggtctgcttt ttgctttgat cgttgtatgt ttacttgttt tgttcttacg tacagaaaca
    37861 ggacttgcta ttcgtgcaac tggcgataat ctggcaatga gcgaagcaaa cgggatcaac
    37921 acagataata tgaagatcat cggttatatg atttctaatg gatgcattgc tctttctggc
    37981 tccttgcttg cgcaaaacaa cggctttgct gatttaaatt ctggtattgg aacgatcgta
    38041 attggacttg cttctattat catcgcagaa gttttgttac gtaaccagcc actatttctc
    38101 cgtctcttga ctgtcgtttt aggtgcggtc atttatcgtt ttattttagc gttagtcttc
    38161 gaactgaatg ttgaaccaag tgattcgaaa cttgcttctg ctttcgtgct agtgatctgt
    38221 ctttctttcc cgcagatcca gcaaaaattc catttacctc aaaaaaagaa aggcggtagt
    38281 gtcaaatgaa tgacgtctta acattggaaa atctccatca tacctttgaa aaaggaacga
    38341 tcaacgaaaa tcatgtcctg aaaggcatta atctttcctt agatcccggc gacttcgtga
    38401 cgatcatcgg tggaaatggg gctggtaaat ctactttgct gaacagcatc gccggtacat
    38461 tttctgtcga tcagggtcaa attttattga atggaaaaaa tatcacgaaa aaatctgttg
    38521 tcgaacgttc gaaatccatc agtcgcgtat tccaagatcc taaactaggg acagccgttc
    38581 gtttaacggt agaagaaaat cttgctcttg ctatgaaacg aggaaaaaaa cgcggcttct
    38641 tccgtggtgt caaaccacaa gaccgaacat ttttcaaaga ccagctggtt cggctaaatt
    38701 tggggttgga aaatcgttta tcctcagaaa tcggtttgct gtctggagga caacgtcaag
    38761 ctattacgtt attgatggcc acattacagc gtccagaact gatcttatta gatgaacaca
    38821 ctgcagcgct tgaccctgct acttccaaaa cggtgatgga attgacagaa aagctgatca
    38881 ctgagcagaa tctcactgct tttatggtca ctcatgacat ggaagacgcg atccgctatg
    38941 ggagtcgact gatcatgctg catcaaggaa ggatcgtcgt agatatccag caagaggaaa
    39001 aacagcagct cacagtgccc caattattag cgttgttcaa acaaaacagc ggcacggcat
    39061 taaaagacga tcaagtactt ttatcttcat aatcagaaag aggatggg
//
LOCUS       DACKAC010000020        37826 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.020, whole genome shotgun sequence.
ACCESSION   DACKAC010000020 DACKAC010000000
VERSION     DACKAC010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.020"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..420
                     /locus_tag="I0B46_001083"
     CDS             <1..420
                     /locus_tag="I0B46_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAR2083397.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(504..1226)
                     /locus_tag="I0B46_001084"
     CDS             complement(504..1226)
                     /locus_tag="I0B46_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAR2083398.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(1216..1953)
                     /locus_tag="I0B46_001085"
     CDS             complement(1216..1953)
                     /locus_tag="I0B46_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAR2083399.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            complement(1965..2957)
                     /locus_tag="I0B46_001086"
     CDS             complement(1965..2957)
                     /locus_tag="I0B46_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAR2083400.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            complement(2972..3715)
                     /locus_tag="I0B46_001087"
     CDS             complement(2972..3715)
                     /locus_tag="I0B46_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAR2083401.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     regulatory      complement(3789..3893)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(3956..5278)
                     /locus_tag="I0B46_001088"
     CDS             complement(3956..5278)
                     /locus_tag="I0B46_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAR2083402.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     gene            complement(5265..6230)
                     /locus_tag="I0B46_001089"
     CDS             complement(5265..6230)
                     /locus_tag="I0B46_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAR2083403.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            complement(6227..7765)
                     /locus_tag="I0B46_001090"
     CDS             complement(6227..7765)
                     /locus_tag="I0B46_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAR2083404.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            complement(8181..8468)
                     /locus_tag="I0B46_001091"
     CDS             complement(8181..8468)
                     /locus_tag="I0B46_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083405.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            8602..9270
                     /locus_tag="I0B46_001092"
     CDS             8602..9270
                     /locus_tag="I0B46_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAR2083406.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            complement(9561..10301)
                     /locus_tag="I0B46_001093"
     CDS             complement(9561..10301)
                     /locus_tag="I0B46_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083407.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(10431..11378)
                     /locus_tag="I0B46_001094"
     CDS             complement(10431..11378)
                     /locus_tag="I0B46_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAR2083408.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            complement(11507..12271)
                     /locus_tag="I0B46_001095"
     CDS             complement(11507..12271)
                     /locus_tag="I0B46_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAR2083409.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            complement(12275..12664)
                     /locus_tag="I0B46_001096"
     CDS             complement(12275..12664)
                     /locus_tag="I0B46_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083410.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            12799..13341
                     /locus_tag="I0B46_001097"
     CDS             12799..13341
                     /locus_tag="I0B46_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAR2083411.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            13489..14121
                     /locus_tag="I0B46_001098"
     CDS             13489..14121
                     /locus_tag="I0B46_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083412.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(14255..14380)
                     /locus_tag="I0B46_001099"
     CDS             complement(14255..14380)
                     /locus_tag="I0B46_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAR2083413.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(14514..15407)
                     /locus_tag="I0B46_001100"
     CDS             complement(14514..15407)
                     /locus_tag="I0B46_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAR2083414.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(15589..16515)
                     /locus_tag="I0B46_001101"
     CDS             complement(15589..16515)
                     /locus_tag="I0B46_001101"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAR2083415.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(16600..17361)
                     /gene="pflA"
                     /locus_tag="I0B46_001102"
     CDS             complement(16600..17361)
                     /gene="pflA"
                     /locus_tag="I0B46_001102"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAR2083416.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(17605..19836)
                     /gene="pflB"
                     /locus_tag="I0B46_001103"
     CDS             complement(17605..19836)
                     /gene="pflB"
                     /locus_tag="I0B46_001103"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAR2083417.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(20113..22563)
                     /gene="parC"
                     /locus_tag="I0B46_001104"
     CDS             complement(20113..22563)
                     /gene="parC"
                     /locus_tag="I0B46_001104"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAR2083418.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(22575..24620)
                     /gene="parE"
                     /locus_tag="I0B46_001105"
     CDS             complement(22575..24620)
                     /gene="parE"
                     /locus_tag="I0B46_001105"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAR2083419.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(24786..25220)
                     /locus_tag="I0B46_001106"
     CDS             complement(24786..25220)
                     /locus_tag="I0B46_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAR2083420.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            25347..25985
                     /gene="plsY"
                     /locus_tag="I0B46_001107"
     CDS             25347..25985
                     /gene="plsY"
                     /locus_tag="I0B46_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAR2083421.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(26117..26992)
                     /locus_tag="I0B46_001108"
     CDS             complement(26117..26992)
                     /locus_tag="I0B46_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAR2083422.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(27074..27865)
                     /gene="codY"
                     /locus_tag="I0B46_001109"
     CDS             complement(27074..27865)
                     /gene="codY"
                     /locus_tag="I0B46_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAR2083423.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(27883..29283)
                     /gene="hslU"
                     /locus_tag="I0B46_001110"
     CDS             complement(27883..29283)
                     /gene="hslU"
                     /locus_tag="I0B46_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAR2083424.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(29297..29845)
                     /gene="hslV"
                     /locus_tag="I0B46_001111"
     CDS             complement(29297..29845)
                     /gene="hslV"
                     /locus_tag="I0B46_001111"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAR2083425.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(29882..30787)
                     /gene="xerC"
                     /locus_tag="I0B46_001112"
     CDS             complement(29882..30787)
                     /gene="xerC"
                     /locus_tag="I0B46_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAR2083426.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(30985..32361)
                     /gene="trmFO"
                     /locus_tag="I0B46_001113"
     CDS             complement(30985..32361)
                     /gene="trmFO"
                     /locus_tag="I0B46_001113"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAR2083427.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(32330..34408)
                     /gene="topA"
                     /locus_tag="I0B46_001114"
     CDS             complement(32330..34408)
                     /gene="topA"
                     /locus_tag="I0B46_001114"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAR2083428.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(34530..35387)
                     /gene="dprA"
                     /locus_tag="I0B46_001115"
     CDS             complement(34530..35387)
                     /gene="dprA"
                     /locus_tag="I0B46_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAR2083429.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(35443..36210)
                     /locus_tag="I0B46_001116"
     CDS             complement(35443..36210)
                     /locus_tag="I0B46_001116"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAR2083430.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(36203..37063)
                     /gene="ylqF"
                     /locus_tag="I0B46_001117"
     CDS             complement(36203..37063)
                     /gene="ylqF"
                     /locus_tag="I0B46_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAR2083431.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            37323..37721
                     /locus_tag="I0B46_001118"
     CDS             37323..37721
                     /locus_tag="I0B46_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAR2083432.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
BASE COUNT        10331 a         7994 c         6490 g        13011 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacaac ctgttgtaca agaaacggtt
      121 caaacggaaa aacaagcagc gcctgttgct gaaacacaac ctgctcctgc tgtaacagaa
      181 actgctgcta ctccagcaag cacaagctct gcaaaagaat ggatcgcaca aaaagaatca
      241 agcggttctt acacagcaac gaacggacgt tacatcggac gttaccaatt agattcttct
      301 tacttaaatg gtgactactc tgctgcaaac caagaaagag tagcagaaca atatgttact
      361 tcacgttatg gttcttggga agcggcgaaa gcattctggg aagcaaacgg ttggtactaa
      421 gattcattta ttaatcaaac agtttacttt tatacaaaga aagccaaaaa gaggtgcgac
      481 actagttgtg tcacatctct tttttattta cctaatctaa tgaaacgttc caagttttga
      541 taagtatttt ttttaacttc ttcaactgat agctctttga tctcagcgat tgtctttaac
      601 tgttcttcaa gcagtgagac ataatctttt tctgaaaaaa tcccccctaa agcccactct
      661 gctgcatgta gtccatccgt ttcaataagt aagtggtcga ttgggactgc tttagctagt
      721 tgccagactg ctggattctt tttcaaatca agaccaatgc tgaaataaca gcctaaatca
      781 atatagtctt gttgatagtc caaagaagaa taccaatgga tcaaatagtg attagggtaa
      841 gcttggatat aatttaagat ctctttttcg catccttttg tgtgcagtac aacaggtttc
      901 tcatttatgg ctgcaaaagc aagctgtcgc tcaaatactt ttttttgtgt agtcatcggt
      961 acattcgtcc aaatattatc taaccctatt tcaccgatga tccttgcttt tttcaaaaaa
     1021 ggctcaactt gttcaaacgt atagctatct gctttccaag gatggatacc gtaacttaag
     1081 gcttgagtct tacttgctgc aagctgtctg ttttccttcc attcttctgg cgaatcacaa
     1141 ttgatgatcg tcaaaatcgt gtgttcctgc tgaaggagtg ctaaactctg actcccatct
     1201 aaatgacaat gagcatcact taacatctaa ttcacctgcc aataactgat aaatcgtttg
     1261 cttcaactct gattgctgca gtagccattc acgtgaccgt cgtttttcag gcacttgaat
     1321 ctcagcaatc acatgtgccg gacgggcgga aagcacaaga atcctgtctg ataaataaat
     1381 tgcttcttcg atatcatgcg tcacaagtaa agtagtccgc tttgcttttt taggcagctt
     1441 caacatccag tcttgcatct gccctctggt catagcatct aacgctccaa atggttcatc
     1501 aagaagcaag taagaagaag gattcatgat tgttcgtaac aaggccgttc gctgcttcat
     1561 cccgccagat aattgttccg gcaaaaaatg ttcatagccg tttaacccaa agatatccag
     1621 ataatccctt acttgttctt cattgattgt tagatgattg attttttgat ataacgtaac
     1681 gttttgatag acgctcagcc aaggaagtaa catatcttct tgcggcatat aactaaatgc
     1741 tgtatggaga ccagttactt tttgatcgtc tactttgatc gttccagaag tcataggctg
     1801 gactcctgtt aaagttttca gcaatgttga cttaccggct ccgcttggcg cgacaatcga
     1861 tgtaaaggta tctttaggga tagtgaaatg gatgtcttct aatattttca ggttttcaat
     1921 gactacattc aagtggctga tttggattga catatctatc actcctattt tataaactga
     1981 ttagtgaata gttcttgcga aggtacatct tgcttgatca atccgttttc ctgcataaaa
     2041 gctgtataat tgcgccaaac tttttcttcc atcaatcccc aattctcagg atcatccgta
     2101 taattttttg acaagaatgc ttgtgattct tctaaaaacg tccgatcatt ttcttttgca
     2161 tatttttgca acagtttagc actgtcattt ggatccttaa tagcttcctg atagcctttt
     2221 ttcgtggcat ccataaaaga ctgtaccaat tctggatcac ttttgatcag ttgatcattc
     2281 gtaataatta ccggagtata gtaatctaac cgttcatcta attctttcaa tgggatatag
     2341 tttagttcaa tacccgcttc ttttgctttg atcatatccc aaccttcaaa gtaccattgg
     2401 atatctgagg attttcctaa gctttctggc ccttctccat tgcttcctac cattgtcagt
     2461 tttgaaaagt ccccgccgtc tttttccatc acagctttta agacagcttc ttcacttgga
     2521 ctttgccaac ctgcatatgt ttttccttca aaatcttttg gtgaatggat gttactgtct
     2581 gatagggtgg cgaatccaga agtattatgt ttgatcactg tagcaattgc tttgactggt
     2641 atattctgtc catctgcgtg agcataagtg acatcttctt gataacttac accaaattgt
     2701 gctttgtccg cacctactag accaatactt gtcgaattat ctcctggttc gatgatttgg
     2761 acatttaaac cagcctcttt ataatagcct ttttcttttg ctaggtagat gccagtatga
     2821 ttagtattcg gtacataatc caaaacaagt gtcacttttt tcaaatcctt tatttcttgt
     2881 ttgctacttg tttcttttgc agtgccacaa gcggataaaa gcaacagtag cggtaataaa
     2941 agccataatt ttttcatttg atccccttct cttaaaaaat ataaagcata cgataatacc
     3001 cttttttcaa taatgaacaa gcgccattca gcaataaact caatagaatg acacaaatga
     3061 tcgcagcaaa aactttatcg atctggtagc tgttttttac gcgtatcatg taatacccta
     3121 agcctgcttg agcgcttagc cattctccaa cgactgctcc gctgacacaa taagtagcag
     3181 ccacttttaa accggaaaag aagccggata aacccgctgg tattttatat attcgataaa
     3241 tcttccctgt atctgccccc atcgttttca aaaagatgat ttgctctttt gatactttcc
     3301 tcaatgtatc cgaaaaagaa actacgatcg gaaaatagct tatcaagaca acaaggatca
     3361 cttttggaag cataccgaaa ccaaaccata gggtaagtaa tggccctaac accatgattg
     3421 gcaacatttg tgaaatcact agtaacggat aaaaagacat ttctaaaaac ttccaataat
     3481 ccattgccaa ggcagtaata aaagaaagaa cgctagctat taccaaacca agcactgctt
     3541 catttaatgt cgtgacagaa tgggaaagta atacttgtgc atctgccgct aatgcagaaa
     3601 atacagcagc aggtccgggt aaaataaagg caggtatttt ccaaactatg acaacaatct
     3661 gccaaataat taaaatgaat gccatactga ctgctggata aagatttttc ttcattttgt
     3721 tacctccttt cttctaatat gaataacaaa aaacgcttga aatgggcacg acatagtaac
     3781 ccgcttcaag cgtctgctat gtttctccct tcaccagtat taactgacag gttcaatggg
     3841 tctaagttca caacttactc tcatgtcgtc tacgacatcc ccagaaacag gtattcattt
     3901 tttaaagtgt agcacaagaa ggaaaaagca acaagcgctt tagttatcat gctgattatt
     3961 tcaataaata ggagagatca cttgccaatg tcggataccc catgatatag ctatccaact
     4021 cttttttagt caatttttga ttgatgacga gtgtcaaatc atcgatcaat tcgtctgctt
     4081 gctcgctaat cactgcagca ccaatcaaat aattttgctg gtcaaaggtc aattcagcct
     4141 tagcaactgg ttcattgaca cgatgatagg tgaaccaact ggtaagatcc atctctacca
     4201 cttgatctga cgaagaggcg tgactttttg taactcctac ttcagcaagt ttaggactgg
     4261 cgtagacgat cgtaggtata atcggatatt tgataggttc gcttgttgca tccgttatcc
     4321 gcttagctac ataatttcct tcaaaagtgg cgactggtgt caattttggg gttttccttg
     4381 aaacaatatc tccacacgca aaaatttttg gattgctcgt ttgcagataa tcattgactg
     4441 caattccgtg tttatcaaag acgatattcg cttgctcaag tgctaaagat tctgtattcg
     4501 gctgtctccc agtcgcacaa aaaatcatat ctgctactaa ttccgtctct tttccgacta
     4561 aacgatagcg agtaccttct gaaatgatct tttgggtttc aacaccaaaa gcaaactgaa
     4621 tgccagatgc ttccatttga tggacggctt cctctaccaa agacgcctca aattctttta
     4681 atggccgttg attatgatgg acaatggtta ctttgctccc tgcagcattg gcaatcgtag
     4741 ctagctcaaa agcaatataa ccaccaccga taaaaatgat ttctttaggc agtacaggca
     4801 atgataaaaa gtctgcacta gttttcaagt attcctttcc ctccactggt aagatcgtag
     4861 gtctttgccc ggttgccaaa actaagtaat ccgcatggaa gacttcttcg ttgacttcaa
     4921 tagatgaatc atccaaaaaa cgagcggttc ctgaaagatg atcgatttca gcttctgcta
     4981 attgtttttt tcggctttct gggactggat ccgtaaatgt tcgtttgaat ttttgaagtt
     5041 cttcccagtt ggcagttggg atctcgttaa aaccttttcc actcaattgt tttacccgat
     5101 tacgtgcctc tactgcactt aacaagactt ttttaggatc acatccacgg ttaggacaag
     5161 tcccgcccca tagatcttct tcgaccacta gtactgtttt acctgcttct ttcaatccat
     5221 aagcagcact taagccgctc acacctgaac cgatgatgat tgcatcatat gttttcattt
     5281 ccttcctcct ctcgacttaa aaaagcaaag atttctttac ttgtcagcga cccaatactt
     5341 ctttgctttt ctactatcac aatgcttgga tacaccgaaa gaagcgaata tacttctttc
     5401 ataggcgtat caaagcaaac ttgttgacga gcattttctg gtatttcatt taagaaatat
     5461 ccattgacga ctaaatcttc tactcgccac tctttcgtct gtttcggttc atcaaagaaa
     5521 tgccgaacgt agtcgttttt aggattcatc aataactgct ctggtgtgtc acattggata
     5581 atttctccat ccttcataaa tgcaatacga tcacctagct tgattgcttc ttccatattg
     5641 tgcgtaacga aaacgatggt cgtgcccaat tcttcgtgta aggacaaaac caattcttgt
     5701 aatgagtttc ttgaaatcgg atctaatgcg ctaaatggct cgtccatcaa aatcacatct
     5761 gggcttgcag caatagctcg gatgatcccg atccgttgct gttctcctcc agacaactct
     5821 ctaggcatac gatctcgata aatatcagga gataagccaa ctttttgaag aagttcatct
     5881 acacggtctg ctcttttctg cttttcccaa cctaacattt cagggatgac ttcaatattt
     5941 tgcttgactg tcatggtcgg gaacaaagcg atttgctgca gcacatatcc catattccac
     6001 cgcattttct gcagattata ttcatttaac gattttcctt tgaagtagat gtctcctgct
     6061 gatggtttag ataatccatt gatcattttc aaagtagttg tttttccact accggaagga
     6121 ccaaccaaaa caaagatttc tccttttttg attgccagat ttaggtcatc gatgacatac
     6181 tttccatcat actttttttg cacatgtttg aactcgatta atttagtcac tgatcttcct
     6241 cctttactaa tccttcattt ttcaggaaga ttcgtgctac ttctgtcggc tttttctttt
     6301 cgacattgac ttggtaattc atatcaatca tttgatcctc tgttattttt ccgcttaatt
     6361 tattcagtgc tttaacgatt tcaggatgtt ctttagcaaa atcttttttc atcagcggtg
     6421 cgccttgata agctgggaat aaccctcgat catcttctag agtcactaga tcgtactcct
     6481 tgagctggct atctgtggaa taggcatcca caatattgac atctccatta ttgattgctt
     6541 ggtaacgcaa acttggctcc atgctttgta cacttgaaaa attcaagcca tatgcttctt
     6601 ggattccttt atatccatct gaacgatcaa taaattccag tgtgaatccc gctttgatct
     6661 gatcagagat agctgccaaa tcggaaatct ttttaagttg atgttcgttc gcaaaagatc
     6721 tcttgactgc caaggcatac gtattttggt aagccattgg ttttaataat gtcatatcga
     6781 attgcttatt caataattct tctgcttcgt catatgtttt ttcttttgtc aaatcagcac
     6841 ttttcattgc ttcaggtacc ttcaccaagc tttctaggac agtacctgtg aactcaggat
     6901 agatatcgat ttgaccttga tccagtgcat tgaacaaaaa actcgtcttg ccaaaattcg
     6961 gttttaattc aacttctgtt tttgtgttgt cttcaatcaa ttctttatac atttggatca
     7021 ggatttcagg ttccgctcct aattttccag caatcgtgat ggtttcattt ttaaacaagg
     7081 cagactgata taaagaaacg ccgccaaata aacacaccac tgctgctaaa gtaatgatgg
     7141 aatatcgagg tttcttatgc tgcaaccagc gaatcagtcc gctgaatata attgcaagca
     7201 aggcagaact gatcgcacca atcaatgtca aaacaggtgt atttcggtca atacctaaca
     7261 aaataaatgt ccctaatcca cctgctccga tcaatgctgc caaagtagcc gtaccgatga
     7321 ttaaaactaa ggcagttcgg atacctgaga taatgacagg taatgcgatg ggaagctcta
     7381 ctttcagcaa gcgtctcatt ctggacatac caaatgctac cgctgcttct tcaatagacg
     7441 ggtcaatttc agctagacca atatacgtgt tttggaaaat cggaagcaaa gcgtaaataa
     7501 caagtgcaat caatgctgga acagttccga tccctacaaa tgggataagt aaacctaata
     7561 aggcaagtga aggaatcgtt tggagcacac ccgtgatctg caataagact tctgccattt
     7621 ttttatgatt ggctgcccaa atcgctaaag gaatagcaat cagtgctgca atcagcaaag
     7681 aaatgagaga aatggacaga tgttgaaatg cagcttccag caactcgcct tttctttcac
     7741 ttaatgtttg tataaaatta ttcatgcgac cttcctttct gttattcttt aagtataaac
     7801 atttctttcc tttttcccaa ctaacttgtc ttgccttaac cttacagcac gaacaaaaaa
     7861 aagaaaaaaa tacctctaaa tatgctgaca aaaaaagaat ccaagcaaca aagtcggatc
     7921 aactttttgg ttctcgtttt ttcactacga ataccataaa aatctatacg aaattgtcac
     7981 ttggaatcag ctatatcttt tttacacaca caacttttat tttaataaaa agaaacagcc
     8041 aataaagtag aaatctcgtg ggggagaaca cctttattgg cctgttcaac gaatattata
     8101 ctgtatcatg acactttttt gccaaaaatg taaaaaatgg ttgttttgac atcataaacg
     8161 tcttgttttc ttatttttac ttatatcacc actttatttt ctatctcttt cactccataa
     8221 agaggaatga gttcttcttt tgactgataa taaacagctt tctcacggtt cttcatcaaa
     8281 aaaagaaaga tgtctttcat agcaagtggt gctttgccgt gttcaaataa aaagataaat
     8341 tgatttgctt tatttatctg gaccattgtc acaggcacca gtttttgttc ttgagccaaa
     8401 aaaactcctt gatactctgt ttttttgggt gattttgtaa gaattgtcaa taagtctgct
     8461 gctcgcattt ttccactcct tatttgataa ataaaaagaa acagccaaaa ataatttttt
     8521 ttcattttcc tcttgaagta agagtataag tcagtataat aaagaagttg ctgaaaattt
     8581 tcaaaagaaa agaggctaga aatgaaaaaa ttaaatttat ttacagaatt gtgcggaatc
     8641 attttaggcg caggaatcta cggattagct gtgacaggaa tcaatattcc atctaagctg
     8701 gcagatggtg gggttactgg tattgctttg cttttgcata atctttttgg attcgctcct
     8761 tctgtcactt cactgctttt taatttacct ttattgctta tctcattgtt tctttttgga
     8821 aaacacgctt ttctccggac gatcgtcgga acatttgcgc tagttttctt tttacattta
     8881 tgggagagcc ttcatataca ttttgctgta gatagtctac tgatcaatag tttgatgaca
     8941 ggtattcttt ctggcattgg ttgcggtctc gtctttcgat tcggcggaag cacaggcgga
     9001 acagatatca tttatcaagc cattgaaaaa tattttcata ttaagattgg caaaagtctt
     9061 ttcttgatta cttttggtat tttagttgtc tccttgcttt atttggatct cacacatttt
     9121 ctgtatacat tacttagctg ttctattcta tcttatacat tgaataaagt gaagtatctg
     9181 gaaattggat ctgttttgcc ccaattggaa gcaaaacaga ctacagaaac atttgaacca
     9241 cttgatcctc ttgaacagtt agagcactga ttcttatttt tcactacttt atttaattta
     9301 cagaaatatc attatgtaaa ctaaaatgat attattcagt aaaatcttct ttataaaaaa
     9361 ataaactatt tttgcaaacg atctgcgaaa atagtttgtt tttttatttt gccattattt
     9421 tcttacttat tgggagaaaa aattcgctta aactcaaaaa aatttgagga tacgatataa
     9481 actttcggat agagtataaa ataaggaaac gccgcagcgt ttcctttcca actgtagccg
     9541 aatataaatt tgtatatcac ttatttaaaa tagctatttt ctcccaaaga gtttccttga
     9601 tcatcgatca atccttgtaa tgagcgttct aagataccat ggcttttttt cgattttttt
     9661 tcttctgact gctttgtttc ctctaacgtt tttgttcgat tgaatgttcg tacagacggc
     9721 tcaccatttc ttttttcttg atacggcgtc ggttctgtat cgaacaacag ataagagtca
     9781 tccgtttttt tcatcgattc gattaaatga tcctcagaaa ttttgctttc tctagggtct
     9841 gggataatcg atgcgggtac atatttagga acaaaataag aacgctcccg caccttcgtg
     9901 tcaacagatg cgtttcgtgt agtatgcttg gactctgtcc ttgtttcact ttgtctgaca
     9961 gaacgagagg aatcaccaaa aagttttttt cgtcctgtcg atgtgatttc tgattggtaa
    10021 tggccggtat aatccggtaa ttttccttta tgcttttcca aattttcctt acgcggagct
    10081 gacttcaatc catttgttgc acgacgcttg cgacggattt tctcggtgtt cgtgttcgtg
    10141 aaatgaaatt ctttcatctg ttcaaacggg gactgattct gttcagtcaa aagatcttct
    10201 tgtccgtcaa ataatactcg cttcttgtcg ttggttaatt ttacgcctaa ctcatcatcg
    10261 tacgcaggaa aacggaaatg ttttcgtctg atttctttca ttcaaaccat ccttctcaaa
    10321 aatggaactt ttctcaataa tagcacagat tcattgtttt cagtagcaat gcgctgatta
    10381 ttttaagaaa atagcgatga atttcataaa tatatatata gtaataagac ttaattcgtt
    10441 gtctctctga taactagttc aggaaaaaaa cgacgataac cttgactcgc ccctttttct
    10501 tctatttttt ttaacaacat ttctccacat acttcaccca tacgtataat atcttgcttg
    10561 atcgttgtca atttaggtga actgatttgg tctaaaaata ccccatcaaa tccaatcaca
    10621 ccaaattctt cgggcaagtg tcctccagca tttataatcc ctcttactgc tcctagtgct
    10681 agccgatctg aactacatac aaaacaagtt ttatctgtaa aatctgccca gttttctgtc
    10741 atatatcttt ctacttctgt cgaatgattt ggaaaccgca agattttact aggaagatca
    10801 acttccttca tcacagataa ataaccagtt tcccttgaaa tttcaaaagg ctcgtccact
    10861 tcgataccga tataaataat cgtttgataa tctctttcta aggcatattg cgttgctttt
    10921 gctgtaccat attcattatc gctgtctaca taatcatatc cttcagaatt ttccccaaac
    10981 aagacaactg gtttttccaa acggtcgatc caatcgaagt ctttttttct gacacctgtg
    11041 atgatgtatc catcacaaga accgacgtcg aaggcatcat ttgtaactaa ttggagagaa
    11101 taatgcgctt taccgatccg tttagcgatt cccatcagca aattcatgta atagggctca
    11161 gttgtgtcga tttcttccag tataaataat ttgatgatct gcgaccgatt cgtgacaagt
    11221 gcttttgcag caatatttgg acggtaatcc agttcagcca ttgctgagaa aactaactct
    11281 ttcaattcgt cagtcacctg ttccggatga ttgatgactc gagagaccgt catttttgaa
    11341 acattggcac gttttgcaac atctgctaaa gtgggcatgc tctcttcctt tctaacatgt
    11401 tgtcattctt actatcaatt ataccaaatt ttcctaacag agtttattat agcccaaaat
    11461 aaaaaacgga aaatcctcta tcgtcatgag tgattttccg tttcaattat ttatactttt
    11521 ttatcaaatt ttttgctaaa aaagtggaag cttcttccat tagtggtatt tgtaccatat
    11581 gtcgctgtgt ttttgaggaa cgaaaaacaa tttcttttcc gtaagactta tgccgatttt
    11641 tttctacaaa tcgttccgtt tgtatataag ggattttttc gtcagcttgt ccatgccaaa
    11701 agaacagagg acgtccagct aacttttccg gctgcaatga caaatcatag ctcttaaccc
    11761 atgaaatcaa atcaaaatag tcttttggta cgacataatt atattccgat gcgcgcctta
    11821 cgatttcttt tccatattcc aaaggtgcag gggaacccat caaacacacg cccgctacta
    11881 tatgaggatg tttagccaac aaggcgcatg ttgtcattcc ccccatcgaa accccgccaa
    11941 ctgcaagctg atcatgtaga agacctcttt tttctaaatg cttgataagt gtgtcaaatt
    12001 caaacagatt tccgtagata ctatcccaaa atgtatagga tggaatagaa gaaaccggct
    12061 gtttgcgctc cccatgattc atcgcatctg gcaggatcac acgaatgccc tgctgcgcaa
    12121 tttttcttcc ttgcgtcaaa actaactcct tacttgtctg ccatccgtga taatagatga
    12181 caacgggaat acgttcctct cttaattctt tcagaacaac ttctaacact ggaatatgat
    12241 ggacagaacg tttcaatata attggattca attgttactc acctattttc attaaatttc
    12301 ctgatcgcca tagcaaccat gctccaacag ttgtctggaa caggttcagt gtaccaaata
    12361 atataaatgc aggactgaat ggctcgggaa ataagcacag agaacaaagt cccaccactc
    12421 ctaatgctag cgaaaggagt ggataaatag aaaaattcaa aaaactggca acgctaccga
    12481 aaatattacc aataaacagt atcaaaaaga aaaaaacgct taaaattaaa ccatctgtca
    12541 gcgacatctg aaaaacaatg ttctctacct caattggctg ttgaatcgaa tagacaccta
    12601 agtatatact gagaaggaga cttagcacgt ggctggcaca aaacacacga ttgaccgttt
    12661 tcattttaat cacctaacct atctttataa ttctattata atcattttat catttttttg
    12721 tatgtataaa gtttcaattt ttcttcactt acgatttttt ttgatattgt tgttatataa
    12781 atgaaaggag ttttacaaat gattgatcca aaaatgttgg ggatgatctt cctcattaat
    12841 tttgcttata tcactctgaa cacgttacgt ttcatgttga caatgaaagg ctatcgactg
    12901 attgctccac ttgtcagtat ggtcgagatc acgatttata tcgtcggctt atcccttgtt
    12961 ttagatcgtt tagataatcc actgaattta tttgtctatg cgttaggtta cgcagtaggt
    13021 atcagtgtcg gaattttgat tgaagacaaa ttggcattag gatacatcat ggtcacaacg
    13081 attttgccag caaatagcgc agaagataaa agtttgccgg gtatactgag agaacacggt
    13141 tatggtgtga cacagacttc tgcaatgggg ctggaaggtg agcggctagt tctcgaaata
    13201 ctttctccta gaaaaagcga acgtgaactg tatcgcctga tcaaagaaat cgaagagcgt
    13261 gcttttatca tttcttatga gccaaaatat atttcaggtg gtttctggac taaaaaagtg
    13321 agaaaacgac aaggaaaata actcacaaac ttaaaaactg tctaatatct aaattaaatt
    13381 ttaaatgtta gacagttttt atttattatt ttgattaaaa aacatcgaca tatactcatc
    13441 aatttgttat tatactttat atacttatca atttaatttg gagatattat ggaaattcaa
    13501 cgtagaacca ctaatttttt acatttaggc tctttttttg caactttgac gatcttattt
    13561 tttctatgtt attcatacct taagacctca catgcgcctt ctagtgcatt aggctggttg
    13621 cttttggatc attctgatat cacagaagcc gaagaaaatc cgtttttttt gattttgtct
    13681 tctctttgcg gcttactgtt ttctgtttat ttcggtgcct atttttttca tcgacatcct
    13741 ggtacgttaa acgaagcttc aagcgtattg attgcattta aaacgttttc ttttttagca
    13801 attctttttt attcgtttaa tcacaatacg cttttcttct ttatcatgaa tggtttagtc
    13861 gtgattttat ccatttttac ttattttatt ttttcactca tttttagcca attgaaatgt
    13921 ccgctcttct ttcgattagt tcctgtcacc tgttttattt acagcttgtt ttcctttttt
    13981 gtccttgctt taatgccact gaccaaccct tcgacgatcc aagtcgcagg aaatctgttg
    14041 gatatgctct tcatcgtcag tctaggtgta tttatgtggc aaagaaaaaa caaacaaaat
    14101 aaaaagcatt ttgaagcctg actattacga cttcataaat gcttttttag ttttaaacac
    14161 ttcaatctaa aagaataacc caagattgga taaattatta atgactcgcc attcgtttct
    14221 tagtgattct ggattatcaa atggacttcc taatctaatc agctagttct tcccataaaa
    14281 tgccatactt gactgaatca taatattgat tatttaaaaa tctaactttt cttatcacta
    14341 cttcctttgt cattccacat ttctcaccat ttttttgcat tcctttgttt ccagaccatg
    14401 tagtgaatct aataggtggt agataggcaa aatactcaaa taattctttt aaccataaag
    14461 gaagagaaag gctcctcctg caagagtcag tataattttt gcttactgct ctcttaggtt
    14521 ttactcacta aaactgttcc atattctttg atataagaaa atgcatgcgg tataaatttc
    14581 ttgctcagct cagaaaagat ttgtcgttta agaaaggcca tttcctccat tgtcaatgaa
    14641 ggatccaatg ttggtaattc aatctcaaaa ttgttttttt cgacaatagg taattctaat
    14701 gtgaatgatt cttcttcctt tatagtattc gtcacaagta gagactgatg gaaaatacta
    14761 ggccggcgat ttttatattt attcttacgt atcctgtcaa cgataacttc tatttgatca
    14821 aaaaagtacg attcatttac gtctccaata agttcggcta attctttgaa tgccttcgtt
    14881 acaggaagct gattcttctg tacaaaaaag taattagcca aagtaagcgg gaagtgttct
    14941 atagaggaaa gtgtgataaa acggtacttc tcatttcctg cagttgtcct cagcaattct
    15001 gtctctcggc tgtttcttac tccataaaaa tacgcttctt tccgttcatc ttcgaaacaa
    15061 tacgcccaaa taacttctcc catcgctaat ttttgttcat caacagagag tctcttcaat
    15121 tgttcagcta tggtttcagc agccgaagtg gcttgttgca agtaatcttt aggatcaaat
    15181 attggaaagt tcaaatgata tcttcgttct tccctcttga ttaatcctgc atcgatcaat
    15241 aaatcaagaa aatgggaaaa tttattttgc gaaaattctt ttttcaattc tcttagaatc
    15301 gtttcttgtt ctcgatgttc atatagataa tcggtcaatg aagaaaaaag cggatcatcg
    15361 gtcagttttt gataaaagtt ttccttattt tttgaataat agttcatata tgttctcttt
    15421 tctatccgtt ttattttact taaaaggctt ttacgtaaaa aggaggaaga acaagagtca
    15481 taattgactg ttattctcct ccatttcttc ggcctttagt atgttctatg tttcttttta
    15541 tatcaatcgc ttatccattc attttgaatg cttcatacta ccatttgatt agctaaatac
    15601 agccgtcaat tgaggtacga cttgtttttt acgtgaaacg acacctttaa gcaaagcacg
    15661 gttgttggtc aaagttgttt ggaaagcttc ttctactttt tcttttcctt taccagcaac
    15721 aagtaattct gaatcactgt ctaagatgtt tgtgacaact aatacgaaca gatcgtagcc
    15781 atttgctttc atttcatctt ccattgctgc ttctagctct gcttgtcgat ccatcacttc
    15841 attaaggtcc actgtattga cttgagcgat acgaacgttg gaactgccca ttgggaaact
    15901 ttttgcatcc aaatcaagaa gaaccgcagc tgatttatca cttagatttg ttcctgcttt
    15961 caacatttca agtccataag tgtttagatc gacttcagcg attgctgcaa gttcttttgc
    16021 agctgcttca tcttcaggcg tacatgttgg tgatttgaac aacaatgtat ctgaaatgat
    16081 agctgagagc atgattcctg cgatttcttt aggaattgtt acttggtttt ctttatatag
    16141 ttttaaaata atcgtacttg tacatccaac tggttctgca cgatagtaca atggattagc
    16201 tgtttcaaag tttgcaatac gatggtggtc tactacagct aaaatattta cttcgctgat
    16261 atctgaaata ctttgttgga attcattatg atccacaagc atgacatctg cattttctgc
    16321 tttagctgac tctactactc gtggtgcaga tagctgaaag tggtctaatg cgtattgtgt
    16381 ttcttcatta ggtgtaccta gagcaactgc ttctgtttct ttacctaatt cattttgcaa
    16441 gtgtgaaaac gcaatggctg ccccgattgc atctgtatct ggattttggt gaccaaaaac
    16501 taaaattttt gacatgtcat tcgctccttt gtgatttctc ctatttaata atagcaaaaa
    16561 aacggaaaga agcaatactt atcttctttc cgatgcgacc tatcgcgtta aatattttgt
    16621 atactcttct gtatgaagca atcgtttagc attttcgaca cgttcttttg taggtggttc
    16681 gatcccttct aatggatatt tcaaaccaag tgtttcccac ttgtattttc ccatcgtatg
    16741 ataaggaaga atctctactc tgtctacatt tgacaaggtt tgaataaagt tgtctagtcg
    16801 gatcaaatat tcatcataat cacttcgctc aggaactagt acatggcgaa tccaaaccgg
    16861 tttgttgatt tttgacaaat attgagccat ttctaaaata ttttcattgt cccaatgcgt
    16921 tagtttttta tgctcttcat tatcgatgtg tttgatgtca aataacagta aatctgtata
    16981 cttcatcaat tcttgaaaac gtgaaaagaa aggttcttca tacgtaaaag gttttccgca
    17041 tgtatccaat gtagtatgca tcttccgctc ttttgctttt ttaaatagtt cgatcaaaaa
    17101 atcgatctgc aatagaggtt ctccaccact aacagtaata ccacctttat ctccccagta
    17161 aggtctgaag cgttctgctt tatctaaaag ttcatctgct gtgtattctt tccctccgcc
    17221 aatattccaa gtatctggat tgtgacaaaa ttggcaacgc atgcggcatc cttgcataaa
    17281 cacgacaaag cgcagtccag gtccgtctac tgaaccgaaa gtttcgatag aatgaacata
    17341 gccgattgtt ttttcttcca tttaagttca catcttttct ttgatttcat tttaagtaca
    17401 agttagctgt cgaagcgaag ctttctgaac aaaatttttc gcaattgccg cttcgttatg
    17461 tgtaaaattt acagggacgg aacatacaag tcctgcccct gtaaatcgtt tctttcaaat
    17521 aagcatactg attattcgat tttgatgcga tggctattca acgtcctttc gacgctaagt
    17581 cgaaaaactt ttttcacgaa ctttttacat tctgtcgtgt gaagttctca tcaagacatc
    17641 taattgttgt tctcttgtca agtcacggaa tttaactgca taaccagata cccggatcgt
    17701 caagttcgga tatttcattg gattttccat tgcatcacgc aataaatcat tactgaatac
    17761 attgacattc aaatgataag ctccttttga gaagtatcca tctaagacat tacgcaagtt
    17821 ttcgattctt gtatcttgtt cttttccaag acctgaagga ttgattgttt gggtatttga
    17881 aattccatcc aatgcacagc cgtaattcat tttagctgtt gagttcaggc tagctaataa
    17941 cccactttgt tccgcattgt aacttggatt tgctcctggc gcaagtggtt ttccagcttc
    18001 acggccatca ggagtatttc ctgtattttt accgtaaaca acatttgaag taatcgtcaa
    18061 taatgaagtt gttggttttg agttgcggta agttttatga cgtttgattt gtcccatgaa
    18121 gtattccaag atccatgaaa ctaattcatc tgcacggtca tcatcatttc cgtatttagg
    18181 gaagtcgcca tccacttcaa aatcaactgc tattcctttt tcattacgga ttgcttttac
    18241 ttttgcatat ttgatcgctg acaagctgtc tgccgcatga gagatcccag caatacctgt
    18301 agcaaatgtg cgttgcaagt cagaatccat caaagccagc tgagctgctt cataagaata
    18361 tttatcatgc atataatgaa tgatgttcaa cgtattgaca tatagttctg ctaaccaatc
    18421 catcatattc atgaagttat gtttcacttc ttcaaagtct agtatttctg atgtgattgg
    18481 acgatattct ggtccaactt gggcgccagt cacttcatcg actccgccat tgatggcata
    18541 taatagtgtt ttagccaagt tagcacgagc gccaaagaat tgcatgtctt ttccgacaac
    18601 tgttcctgat acacagcaag caatcgcaca atcatcagaa ccccattgtt cacgaagctg
    18661 attatcattt tcgaattgaa tcgaagaact ttcgatagcg attctgctag catatgttct
    18721 gaatccttct ggaaggtgtt cagaatacaa aacagtcaag tttggttctg gtgaaggtcc
    18781 catatttgtt aatgtatgaa gcagacggaa atcattcttt gtaactaatg aacgaccatc
    18841 taatcccata cctgcaatgg ataaagtagc ccagataggg aatcctgaga ataattggtt
    18901 atattcaggt gtacgcgcga atttgaccat acgtaatttc attactaagt ggtcgatcaa
    18961 ttcttgtgct tctttttctg tgattactcc attatccaaa tcacgttgga tatagatatc
    19021 aaggaatgct gagatacgtc cgattgacat cgcagcacca ttttgtgatt tgattgctgc
    19081 taaatatccg aagtatagcc attggacagc ttcttttgca tttgcagctg gtttagaaat
    19141 atcatagcca tatttcgcag ccatttcttt catttgcgct aatgcacgat attgttcagt
    19201 gatttcttca cgtaaacgaa tcacttcttc actcattgta tgataaccag tcatatcatg
    19261 atcttttttc ttttgttcca tcaaatagtc gattccatac aatgccacac gacgatagtc
    19321 accgatgata cgtccgcgac cgtaagcgtc tggaagccct gtaatgattt tgtttttacg
    19381 agcagcacgc atttcagcag tataagcgtc aaatacacct tgattatgtg ttttgcgcca
    19441 gtcagtgaaa atatgtgtca attcatcgtt tggtgtatag ccattgcttt ttagcgcatt
    19501 gttagccata ttgattccgc cgaacggcat gaatgcttgt ttcaaaggtt tgtctgtctg
    19561 tagaccgacg attttttcca aatctttatc caaatagcct gcatcgtgtg aagtaatagt
    19621 tgatacgatt tctgtgtcca tatcatagac accgttacgc tcgaattgga tatcatttaa
    19681 cttttgcaat tcatcccata atttttctgt tgcttctgtt gggccttcca aaaagctgtc
    19741 atcaccgcga tattcagtgt agttattttg aataaagtct ctagtgtcta cgccttcttt
    19801 ccatttggta cctttaaatc cattccattg ctccattgta tggacctcct aaatttctgt
    19861 tttatgtaac agtttttacg tgattagtat aacacagcac cctatctttg caagggtttt
    19921 cttgtttttt atttaacaat ttttatgttt tatttacata tatttaaatc aacgttgaca
    19981 taccaacatt taagcattgt gaacactttc atcttttttt gcaaacgtat aaggatttat
    20041 taaaaatgaa tgaaaaagag acgccacctg tattaataca gatggcgtct cttcattaaa
    20101 atactacaac agttacgact cgatgaaaat ctcaggagca tcgataactt cgatgacttc
    20161 tccacctttt ttctcatcca tcgcaaatga tccattcgat gttcgatcac ctatagggaa
    20221 atcagttggg tcaatcgtta actgctgccc tttttgattc atcaaggtta tttcttttga
    20281 tcttccttcc gacatataga taatacggtg cggatttttt ttcagctcac gcatgaccgt
    20341 cactccccgc ttcgcacgtg aagtttggct gatttcttga gcaagcatgc gtttaacagc
    20401 tcctcgctga gtaacgatta cgatcggcgt atcaccttct gctaacacaa gcagcccgtt
    20461 gataatccta tctccatctt tcaaattcat agacttcact ccagctgcct ttgctccaac
    20521 aacgggaaca tctgttaaag gatagcgcaa acccattcct tgatgcgtta ctaagaaaac
    20581 atctaagcct ggctgatctt ctttcagata cacagcaatc agttcatctt cttccgcctt
    20641 tagcttcata cagctgagcg gacggctctt ataggtgcgc catggttcga actctgtcat
    20701 tcgtgtttgc ttgatcaatc cattcttgct gatgaataca aatgttttct ctgcttgaat
    20761 tttttgatat gggaaaacag ctaaaatcga ttcgtctaca gacagattgg agattgtttg
    20821 agaaatgtgc tcaccagcat ctttccattt caaatcgggt agttcatgga cagggcggta
    20881 gatcacattt gctttatttg tgatcagaag aacatggtcc aatgtactca attcacctga
    20941 gtatagcaag tagtcgcctt ctcgcatccc gatttcttct ggtttggaag cactataaga
    21001 acgaaggctt gttcgcttca catagccttc tcgcgtcaca ctgacaatta cgtcttcttg
    21061 tgccactaag acttgcgtat caattttgat ttcttcgatt tcgtcttcga ttgtcgttag
    21121 acgagcggaa gaataatttt ttttcacttc tcgcaattct tttttcataa cagagaaaag
    21181 ctctttgtca tttgaaagga ttttattcaa ctctgtgatt tgcgcaatca agctttcgga
    21241 ttccttttga agctctgtga tatccgtgtt cgtcaaacgg tataattgca atgtgacaat
    21301 tgcttctgct tgttcttctg taaattgaaa gacgtcgact aaattctttt tggcgtcttt
    21361 tttgtcttta ctttcacgaa tcgttgcgat tacttcatcc agaatcgata aagctttcat
    21421 caatccttct acgatatgct gtcttttttg agctttggct aaatcgaatt gactacgttt
    21481 tgttatgact tgctttcgat gctcgatata acttcttaaa atgtctttca gtccaacttg
    21541 atgcggagtc atatggtcga tagcgaccat attgaaattg taattgatct gtagctcagt
    21601 gtttttgaac aaataattca aaattccttg agcattggcg tcttttttca attcgacgac
    21661 aatttgcaga cctgttctat cactttcatc tcgtacttca gctattccat cgattttctt
    21721 attcaaacgg atctcatcca tttttttcac caaggtcgct ttatttactt catatgggat
    21781 ttctgtgatc acgatttgct gtttattacc cttcattgcc tctatctttg ttttcgaacg
    21841 aagaataact tttcctcgtc ctgtttcata agcttttttg atttcatctt ttccttgcaa
    21901 gatccctcct gttggaaagt caggtccagg aatatattcc atcaattttt caaggctcgc
    21961 ttgtggatga tcgatcatat agattgtgcc atcgatcact tcagctaagt tatgagtcgg
    22021 gatttctgtt gcatatccag cagatatacc tgtcgatcca ttgacgagaa ggtttgggta
    22081 tcgagctgga aggactgttg gttctttttc tgtatcatca aaattccaca cgagatcgac
    22141 tgtttctttc tcgatatctg ctagcatctc tccacttaac ttggataatc tggcttcagt
    22201 ataacgcatc gcagcaggtg gatcaccgtc catactcccg ttattcccgt gcatttcgat
    22261 cagtacttcc cgcaacttcc agtcttggct catgcggacc atcgcttcat aaatactgat
    22321 gtcaccatga ggatgataat tccccatgat atttccgaca gattttgctg atttacggaa
    22381 tcctttgtca tacgtattgc catctttgtt catggcaaat aatatccggc gttgcactgg
    22441 cttcaatcca tcacggatat caggcagtgc acgttcttgg ataatatatt tggaataacg
    22501 tccgaagcgg tcacccatca cttcttctaa agttaattct tgtacttctt gtcgattttc
    22561 catcaggtga cctcctattc tacttcaaat aactgattgt tttcattttt atctgctcgt
    22621 tcttcatcta ataaatcatt agaaactgaa ggagagattt cagtatcctc ttttttatct
    22681 aagatactgc catcttcttc gagcgtaaat tgcacgtgat tttcgatcca ttttcttcga
    22741 ggttctactt tgtcacccat cagtgtggtt actcgacgtt cagcttgcgc agcatcatcg
    22801 atacggacgc ggatcaacgt acgggaagtc ggatccatcg ttgtttccca caattgttcc
    22861 gcattcatct ctccaagtcc tttatatcgc tgcagcatat atcctttgcc gactttttta
    22921 atcattgctg ccaattcgtc atctgtccac gcatattcaa tgacttgctt ctttccttgt
    22981 cctttggata ctttatataa aggcggtaac gcgatatata ctttcccagc ttcaatcagc
    23041 ggtttcatat aacgataaaa gaaagtaagc agtaatactt gaatatgggc gccgtctgta
    23101 tccgcatcgg tcatgatgat tactttgtcg taattacagt cttcgataga aaactcagga
    23161 ccgactcctg caccaatcgt atagatcatc gtattgattt cttcattttt caagatatct
    23221 tgcattttag ctttttcggt attgatgact tttcctcgta aaggtagaat tgcttggaac
    23281 tttctgtctc ttccttgttt agcagatccg ccagcagaat ccccttcgac taaatacaat
    23341 tcatttttct tagggtttcg ggattgtgcg ggtgtcaatt ttcctgaaag aagagattcg
    23401 ccttttttac gcttcttccc atttcggctt tcttctctgg ctttacgtgc tgcttctctc
    23461 gcttctctgg ctttgattgc tttacggacc aacatctggc tcatttcgct attttcctgc
    23521 aaataaaagc ccatttgttc actgatgacg ttatctacta cggtacgagc aactggcgta
    23581 ccaagttttt cttttgtttg cccttcaaat tgcagaagat tttcaggtac acggatagaa
    23641 agtacagcag ctaacccttc tctgaaatca ctaccttcca gattcttgtc ccgttctttc
    23701 agcaaaccaa cttttctcgc atattcatta taagatttcg tcatcgccgc tttcatacca
    23761 gcttcatggg ttcccccatc ttttgtccga acgttattga caaaagataa aacattttct
    23821 gaataaccgt cattgtattg ataagcgacc tctacttcga taccttcttt ttctcctgaa
    23881 aaataaacaa ctggtgtcaa tgtatctttt tcttcattca gataatcaac aaattctttg
    23941 attccttctt cgtaatgaaa aatttccttg actggttctt ctccacgaag atcagaaaga
    24001 gagattttaa cgccttttag aagaaaggca gattctctta accgttcggc taaaatttca
    24061 tatgaaaatt tcgttgtact gaatattgta tcatctggta aaaaatggac agtcgtcccg
    24121 tttgcttttt ttgtttttcc tatctttttc aaagtaccgt caggtttacc gccgttttta
    24181 aatttctgct gatactctac gccatctcta acgatcgtta caattagcca tttcgataag
    24241 gcatttacca cactggctcc tacaccgtgc agtccaccag aagttttgta ccctccttga
    24301 ccaaactttc cgccagcatg cagcacagta aaaatcactt caactgttgg tattccggaa
    24361 gcatgcattc ctacaggcat cccacgtccg ctatctgcaa cagttatact attgtcttca
    24421 tggatggtca catcaatctc actgccataa cctgataatg cttcgtcaac tgcattatcg
    24481 acgatttcat agactaaatg atgcaagcct cggctgtcag tggatccaat gtacatccct
    24541 ggtctttttc taactgcctc taatccttcc aatacttgga tggacgagtc attgtattcg
    24601 ttgtttactt tttttgccaa ggcaaacgct ccttcaataa tactttgcgg caaaaccgca
    24661 ccacgattat catactagaa aaggtcgtga aaaaaaagag ggtagacgag aaatcaattt
    24721 taattatctc ctaaccgctt tttcgacatt cccctctatc gatcatctct tctttgtggt
    24781 catttttatt tttggttttc tttcaactta ccaagttcaa tcttaataca acgatccatt
    24841 acgatatctg tacgtcctgc ttttcttagc atctcagctg cttctttgtt ttcaatccct
    24901 aattgtgccc agaacacttt agcatcagtt ttcaaaaaat cacctgctat ttcaggtaaa
    24961 aattcactcc gtctaaatac atctacgata tcgattggat catcgatttc tgtcaaattc
    25021 gcatagactt tctcgcctag aatttcttca ccagccaata atggatttac tggtatgatt
    25081 ttatatccat tgtcttgcaa tacctgtgca accatgtaac ttgttcgttc tggtttattg
    25141 ctcaagccta cgacggcaat attttttgct tcttccaaat agttgaaaat cgtttcttgt
    25201 gttggatttt ttacagacat ttctgccacc ccttgctttt ctttttattc catctattat
    25261 acttgtatct ttcatttttt caattgttcc tgttacctat tcatgctaaa ataaaggata
    25321 gttttcaact tgaaagaggg taaaaaatga aattgatctt attacttatt ttagcatatc
    25381 ttttaggttc tattccttcc ggtgtctgga ttggcaaatt gtttttcaaa aaagatatcc
    25441 gacaacacgg gagcggtaat actggaacga ccaatacatt tcgtgtatta ggcaaaacag
    25501 ccgggattgt tgtcctgctt atggatattt taaaaggtac attggctact tgtttgccga
    25561 tgatcttcca cctttctggg attaaccctc tttggtttgg ggtatgcgcc attcttggcc
    25621 atacatttcc gatttttgct gggttcaaag gaggaaaagc tgttgcgaca agtgcaggaa
    25681 tgcttttagg atttaatcct gtattttttg tttattcttg tatcatcttt atcgttagtt
    25741 tattttgtac aagtatggtg agtttgagca gtatgatcag tgcagtattg atcactttat
    25801 ctacgatcgt ccttccttat gtagctcctg tgattttggc taagcccaat tggttactga
    25861 cgatcattgc ctttttagtt tcttccttta tcttttatcg gcataaagac aatatcaagc
    25921 gtatcagaaa ccatactgaa agtcgaatcc cgtttggttt gaatagcagc aagaaaaaat
    25981 cctgatactt tttcattcca aagagataaa acgggagtgt gacaaatcga tgtcacactc
    26041 ccttattact tacaggatag cttttttaca caccttggta aacggtgttc cctgctaact
    26101 tctcggtcac aacctcttac ttcactgtga tggaatattt tgtcttgaag atttcatggg
    26161 cacctagttg attgatccct actttttcca tcaagtttcc actactgttc accgtgtcag
    26221 cgattccgca ccacggttcg atacaaacga aaggcgcttc ttttggataa ggagaccaga
    26281 tccctacata aggcatatct ttatagctta aagttacgct atgggaagat ttttcacttc
    26341 gaatggcgta cgcatttaga ccccttgttt caaaaaccaa ggcatcttgc tcaaaaagtt
    26401 cacgattcaa agccagattc gtatttgtct gacctaacgt cttttgatcc atatcgatga
    26461 atgggccttt caatggtagg cgtaatcttg atttcaatgg agaaaatgcc agatagaaat
    26521 cttcaaatga caatccttct tcaagaggta cattgaaagc aggatgtcca ccgatagaaa
    26581 agaacatttc tttttcaccg gtatttgcca cttgataatg aaccgtgatg ccgtctcctc
    26641 ctaattcata gctgatcact aattcaaaat caaatggata cttttctaat gtctccggcg
    26701 tactccttaa gatgaaacta gccatttctt gtccatgttc aatgacttca aaatccatat
    26761 cccgagcaaa tccgtgttgc cccatttgat atgttttccc ttgataaaca tactgatcat
    26821 cttttaaacg tccaacaaaa gggaataaaa ccggtgcatg tctgccccaa tacgctggat
    26881 caccttgcca gatatactct aaaccatgtt cttttgattt cagactgatc aattccgcac
    26941 cgtgttcagc gatttcggcg atcagctggt cattttgaat aataacagtc attgccattc
    27001 ctccaacttc gacttacttt ttaaaatggg aaggctggaa caaacatttc tatctgcccc
    27061 agtcccctta cttctaataa ctgtgttttg ctaactcgtc tttaaaacgg gaattcaata
    27121 cttttaaata agttcccttc atgcctaacg aacgagattc aatgattcca gctgattcca
    27181 attttcgtaa agcattgacg atcacagaac gagtgatgcc gatactatcg gcaatattag
    27241 aagcagtcag acgaccttca tctccatcca atgcttcaaa gatagcttgt acagccttca
    27301 attcgctata agataatgtg ttgattgcca tttggacagc cgttgcactc cgtacatctg
    27361 cttcgatgct tcgagacttc tgataaagga tctgcatacc gacaaccgta gctccatatt
    27421 ctgctaagac tagatcgtca tctgcaaacg attgttccat acgagataag ataatcgtac
    27481 ccaaacgttc gccagctcca aagatcggaa cgacagtcgt cagaccaaat ggatatttct
    27541 ctcttgactc taccggaaat gcagtaatat cactagtaat tgggatatta gcttcagttt
    27601 tcatcaactg tgccactgta tcagtataag attcaggaaa ttgtttctca acaaacattg
    27661 ttttgattcg ttcattgttc acatcgtgac gttcattata acctagtaat actccgtcac
    27721 ttcctatgat ataggtgttg ctgtcaagaa taccacctag tgtgacagcc attttatcgt
    27781 aaggcaaatc tgcttgcatg tcaaaagtat tcttttgctg caaaagtgtg tttatcttgc
    27841 gggttttcgt tagcaagtct gtcattgtcg tcctccgatt ttttataaaa tatagcgact
    27901 taaatcttca tttgccgcaa ttgagccaag tttgtcatcc acatatgctt ctgtgatcgt
    27961 tatttctccc atttgcatat ctggtgcttc aaataataga tcttctagta gtttttccaa
    28021 aatggtatgc agtctgcgtg cgccgatatt atctgtgtca cggttgacat cgaaagcgat
    28081 ttgagcgatc cgttcaattg cttcttttgt aaacgtaact gacacatttt ctgtaccgat
    28141 caacgcgacg tattgtttta ctaaagcatt atttggttcg gtcagaatct tcacaaaatc
    28201 ttcagctgtc aaatcatcta attctacccg tatcgggaag cgtccttgaa gttctggaat
    28261 tagatcactt ggtttagaca aatggaaagc tcctgaagca atgaatagga cgtgatctgt
    28321 ttggatgatc ccgtattttg tgtttacttg tgaaccttcg acaattggca atatgtctct
    28381 ttggacgcct tctctggata cttctccaga ttgctgtgat ttagaagtga tcttatcgat
    28441 ttcatcgata aaaataattc cactttcttc cgctaatttg attgcctcac tatggatatc
    28501 agcatccttg actaattttg ctgattcttc cttgatcaat aactctaaag cttctttgac
    28561 tgttaccgtc cgttccactt ttttcttcgg tgtcaaagca cctagagttt catttaggtc
    28621 gatccccatt tgctccaatc cattgttcat cattggtgca ggtttctttt ggtcttcaac
    28681 ttcaatggtt acttcacgac tatccagtaa acccttttgt agttgttcca agactgtacg
    28741 gcgattgatt cgaatatctt ctgtcacttc ttcttgcggt tcagcggtat tttgctgact
    28801 agcattaaaa agattcatca tttgttcaaa ttgattagtg gattgtttct gttctttttt
    28861 gataccaggt accaagattt ttaccaaacg tttttctgca ttttttttag cacttgtgta
    28921 tacacggctg tactgctctt tttctacgat ttggatcgca ttttctacta aatcccgaac
    28981 cattgattct acatctcttc cgacataacc gacttcagta aattttgttg cttcgacttt
    29041 gacaaatggt gcattgacga tttttgccaa acgtcgtgcg atttctgttt taccgacccc
    29101 tgtaggtccg atcatcagca tattttttgg tgtaatatct tgttgcattt tttcatctaa
    29161 ctgcaaacgg cgatagcggt tacgcaaagc aacggcaaca gatttttttg ctgcgtgctg
    29221 tcctacgata tattcatcta attcacttac gatttgtctt ggtgtttgtt ttaattcata
    29281 catatctact cacctcttac agttcttcta cgataatatt atgattagta aagatacaga
    29341 tatctgcggc aatatttaac gcattttctg caatttcttt cgcactcatg tcgttttgag
    29401 catagttttt cattgctcgg gctgcagcca acgcaaaatt ccctcctgat ccgatagcta
    29461 agataccatc atctggtgtg ataacttctc ctgtacctga aacaagaagc atttctttat
    29521 cattcattac gatcagcatc gcttcaagtt tttgcataga ttgctgtgtc cgccattctt
    29581 gagctaattc gacagctgcg cgttgtagat tcccgttata ttcgtttagt ttgccttcaa
    29641 atttttcttc taaagtaaac gcatctgcaa cacttccggc gaaacctaca acaacttctc
    29701 cattgtagat acgtcgtact ttctttgcag tgcctttcat gacaacagat tcacccattg
    29761 ttacttggcc atctccagcc atagcaaatt tcccgtcttt ttcgacggca cagatagtgg
    29821 ttgaatgaaa tggtgattcg accatgtttg tttcctccta aatttgttct tttccttatt
    29881 tttacgctct tggatggaaa gaacgatagt ttttttgtaa actttctttt gttacatggg
    29941 catagatctg ggtagtcgat aaattggcat gtcctagcaa ttcttggact gtccgcaaat
    30001 ctgccccatt atttaataaa tgagtagcaa atgtatggcg cagcatgtga ggatggatat
    30061 cactatccaa actgcttttt ttgatcattt gattcaagac atattcaatt ccagtaggcg
    30121 tgattggttt tccatgatga ttcacaaaaa cgatatcatg ctcttcgtga tattttgtca
    30181 tcaatacctg gcggccttcc ctgaaatatt cttgcagagc atcttgagca aaagaaccta
    30241 gaggagcata tctctctttg ttccctttac catggatcaa catgacctct gaatcaaaat
    30301 caatgtctga taccgacaaa ctgctacact cacttaaacg gatgcctgac ccgtaaagga
    30361 cttccagtaa agcgcggttc ctcaaatcca aaggactatc tcctgctacg ctgtcaaaaa
    30421 gtgcttgcat ttcattttca taaaaaaaac gtggcagtct caaatttttc tttttgagat
    30481 gaacgtaaga aaatggattt tcttttatca cttcttcttt gagaagatac tgataaaacg
    30541 aacgtaggct ggctatcttg cgactgatag aatttctgct atagctagga cgatgatcag
    30601 ctaatgcact caaatagaca cgaaccgtaa aatattccgc cttcagcaga tcagcgtctc
    30661 cagattcctc caaaaaatgt ttgaaatgga gaatgtcttc ttcatatgct tctttcgttt
    30721 tttctgaata tcctctttcg acaatcaaat aacgcaaaaa tttttgttcc cacgtggctt
    30781 ctgtcatttt ctcaccttcc ttttgtccca gagaataatt tagcacagcg tttataaaaa
    30841 gacaaataaa ttgtcagaat ttaaccaata tttctaagtt tgttcacaat tggcgcaatt
    30901 cctcacattt tgtttgagaa attcttaaga aaaacgcaag aaaaagagcg agactatccg
    30961 tctcaccctc ttcatggagt ttccttactc atttgggatt gcagtttttt ctaattctgt
    31021 ttttattgcc tcattagctt ttaaagctcg ttgtgcaatt gcttcatatc ttgctttttt
    31081 gtctcgaatg cgctctggca gttcaggaaa cagaccatag ttggcattca tcggttggaa
    31141 atgttttcct tccgcatgag taatgtagta agccatactt ccaatagctg tttcttgtgg
    31201 aaactcgact ggttccattt cttttgctaa gcgtgcggcg ttgattccag caagtagtcc
    31261 gctcgctgca ctttcaacat agccttctac accagtcatc tgacctgcaa agaacagatc
    31321 ttcgcgtttc gttgattggt aagtcggttt cagcaattct ggcgaattca taaagctgtt
    31381 tcggtgcatc acaccatagc gaacgaattc agcattttct aacccaggaa tcatccggaa
    31441 aacacgtttt tgctcgcccc atttcaagtg agtctgaaag cccacgatgt tgtataaaga
    31501 agctgcagca ttgtcttgac gcagctggat aacagcataa ggacgtttgc cagttttagg
    31561 atcttccaat ccaactggtt tcataggtcc gaaaagcatc gttttgaatc ctcgctgtgc
    31621 cattacttca attggcatac atccttcaaa atatttttct ttctcaaaag atttaagttc
    31681 tgccacttcg gcattgatta gtgcctcatg gaaagctttg aattcctctt ctgtcatcgg
    31741 acagttcaag taagctgctt ctcctttgtc atagcgggac ttcaaatata ctttatccat
    31801 atcgatcgta gctttgtcta cgattggtgc agctgcatca tagaaataaa aaccatctga
    31861 accattgaat gctttgatgc tttctgctaa tggatgagaa gtcaaaggtc ctgttgctac
    31921 tacggtgatt ccttccggta tttcagtaat ttcttcgttt atcactgtta ctaatggatg
    31981 ctctttgact tttcgggtaa ttgtttcaga aaaagaatcg cggtcaacag ctaaggcacc
    32041 accagctggc actgctgttt catctgccga agtgattact accgaattca atcggcgcat
    32101 ttcttctttc aataacccta ctgcattagc caaactgttt cctcttaatg aattggaaca
    32161 tactagttca gcaaaattac ttgtgtgatg cgcctcagtc gatttgactg gacgcatttc
    32221 atataaacgg acagggactc ctgcttgtgc gatctgccaa gcagcttcac ttccggcaag
    32281 ccctgctcca acaacattta ccattttcaa tgaaagaacc tcttttctat tatttttgga
    32341 tgtcttcttc gtaatctcca tttacacaga ctacttgttt accgcctttg actttcttct
    32401 cgaccagata tccgtcacat tttggacaat ttctgcccac tggtttatcc caagaagtga
    32461 attcacattc tgggaaacgg gaacaaccat agaatagtct gtttttctta gattttcgtt
    32521 caatgacttg tccttcatga catactgggc aagtcacacc gatttctttt acgattggtt
    32581 tcgtattccg acaatcagga aaattactgc aagcataaaa cttcccatat ttccctaatt
    32641 ttatcaccat cggatgtcca cagacatcac aatcgaatcc tgctggttca tctttgattt
    32701 gaattttttc gattttttct tccgcattcg tcagttcttt ttcaaacggt ttatagaaac
    32761 gatcaaccac tttgacccac gcttccgtgc cttcttcaat tttatccaaa tctccttcca
    32821 tttctgctgt gaaatggata tcgacgattt gagggaagaa ttcacagatt aatgaattga
    32881 caatttctcc tagttcagtc ggctcaaatc ttttttgtgt caattttaca tagtagcgtc
    32941 tttggatcgt atcaagtgtt ggtgcataag tggaaggtcg ccctaccccg ttttcttcta
    33001 gtgcacgaat caacgtagcc tcactaaaac gtgctggtgg ttgagtaaaa tgttgttttg
    33061 gttcaatatc aacagattgg actacatcgc cttcttcaag ttctggaaga atattttctt
    33121 tctcttcttt gccgtcatct ctaccttcca catacacttg catgaatcct ttgaatttga
    33181 tttttgaacc gttcgcaatg aagatgacac cattttgttc taatgtcact ttcattgtat
    33241 ctaaaatagc tggtgtcatc tgactagcca caaaacgtga ccagatcaat gtataaagtt
    33301 tcaattgatc tttatctaaa tattgtttga gttcatctgg tgtacgaagc acactcgttg
    33361 gacgcacagc ttcatgggca tcttgtgcgc cttgagcatt tttgatcttt cggtgatcat
    33421 gtctagaaaa ttcttttcca tacgtatctt caataaatgc tgcggcctct gcttttgctg
    33481 tatcggcaat ccgggtagaa tcagtacgca tgtatgtaat caagccgact gttccttgtt
    33541 tacccaaagt gattccttca tataattgct gtgctaccat catggttttt cttgttcgaa
    33601 agtttaactt acgggcagct tcttgttgca ggctacttgt agtgaaagga ttcgcagggt
    33661 ttcttttgcg ctctttcttt tctacttttt taacttcgaa gtctgttcca tcaattctag
    33721 cagtgacttc ttttacactt tgtgcatctg gtaatttctt tttcttaccg tcgattcccc
    33781 agaaatttgc tttgaatttc ttgcgagctt ttttaaaatt accgtcgatg ctccaatatt
    33841 cttctggaac aaattctcgg atctcttttt cacggtcaat aataattttt aaagcgacag
    33901 attggacacg tcctgcactt aaccctttct tgaccttacg ccagagaata ggactgatcg
    33961 aataaccaac gattctgtct aaggtacgac gagcttgctg tgcatctact aaatctacat
    34021 cgatcgttct tggttcctta aaagctgctt tgactgcgtc ttttgtgatt tcattgaaga
    34081 ccacccgatt tttttctttc agatccaacc caagtaggta cgctaaatgc caagcaatcg
    34141 cttctccttc tctatccggg tcacttgcca agtaaacttt ttgagctttt tttgctgcgg
    34201 ctttcaggct tttgatgaca tcgccttttc cgcgtataga aatataatgg ggttcataat
    34261 tgttttcaaa atcgataccc attttacttt tcggtaaatc tcttatatgg ccaacactgg
    34321 ccattacttt atagtttcgt ccaagatatt tctcaattgt cttggctttt gcaggtgatt
    34381 ctacaatcac taaatattta tacgccatta ctgaatggac tcctttccat tgtcgtgttc
    34441 ctactattaa aagaggattc atacaaatcg tagctaatca tatccattca gaaaacgttt
    34501 gtcaagaaaa gcaaatgaaa agtatggtgt tattctttaa attctgccaa gatatcttca
    34561 cccgaaaccg tgcaaactgc accgtcttgg atcaaatgat gacatccggt agaatgttct
    34621 gttaaaatat ttcctggaac agaaaaaact gttcttccaa gatccaatgc ttgctgtgct
    34681 gttatcagag agccgctttt tttcttggct tcaaagacac atgttccttt agacaaagct
    34741 gcgataatcc gatttctcat tggaaaacaa tgctttttcg ggccggattg aggaggatat
    34801 tcactcaaaa gaagatgatc atttgcaatc acctcttgca agtcacgatt ttcagcagga
    34861 tagacttgct ctatccctgt tccaatgact gcgattgtcc gtccaccatg agagattgct
    34921 gcttggtgac cgtatgtatc tatccctttc gcgcagccac tgacgatcac atatccagct
    34981 tctatcactg gttttagtaa ataataaact gtttttttgg ctaaagcaga agtgtgtctt
    35041 ccaccaacaa cgcttaacat gttacaagaa agtaaagata gatcaccatt ataaaacagt
    35101 acaattggcg gataggtcaa gtgaagcagc tgagggggat atttgggatc aaagcaagtg
    35161 ataaacgact gtttcgattg gattttgccc cagttagcgt gaatttcctg ccaacttttg
    35221 cgaaacagct cccggtgttt ggttatttta cctatcttaa tgatgtcttc aaatccgatt
    35281 tctttataag gatactgcat catccagtta gcaagtgccc attttccttg actactgatt
    35341 ccttttgtat aagcaagttt caataaattt tcttctattt gatacatcat aaaaccgcct
    35401 ttcgtctctc tttctaataa gatacgtaaa agcggctgtt ttttattgta aatatttttt
    35461 tacaggtgca aaacttttac gatggattgg cgtaattcct tgtcgttcaa gtccttctaa
    35521 gtgttccttt gttccataac ctgcattatg cgagaaacca tatccaggat aaagcacgtc
    35581 atattctttc atcaattggt ctcgatatac tttagcaatg atacttgcag cagcaatcga
    35641 cacagatcgt gcatcccctt tgattatttt ttcctgctga ataggcaaat ccaaggtcat
    35701 cgcatcgatc aacaaatagc ttggtacaat ggacaatttt tctactgctt ggatcatcgc
    35761 taatttactt gcttcataaa tattcacatc atcgattgtc tgctgatcaa cgatcccgat
    35821 cccgattgcc agggcttttt cttgaacttg ttgataaaga agttctcgct ttttctctga
    35881 taattgtttg gagtcattca gccctaatat gatctcattt ttcggcaaga taactgcagc
    35941 tgccacgaca ggtccagcta atggtcctct tcctacttca tcaatgccgg caatccattg
    36001 gtgaccttgt gcgtaccctt gcttttcaaa gcaaaacatt tcttcatatt tttccttaat
    36061 taatgcttcc tttttcaatc gtcgttcaaa agaagccagt gcttgctgca cgcctttgcg
    36121 tggatcattt cttaatgatt ctagtgtagg atcatctatt ctctgtatgc ttttcaatcg
    36181 ttctttgata ctttggatgg attcagtcat cacttttttg ctccatttct tcgcaacgat
    36241 ccagtgtgta tcgtccaagt tttccttgac gaatatcatg cacgactaat tcgctgccac
    36301 gatcatagtc ttctttatac ccacgttttg ctgtaatatc catcagtaat tccggtgcca
    36361 atttttcttc ttgctcgatt gataaaccat aacgatcgct gattcttcct gaataatatc
    36421 gggcaaaaaa gtctaatccg taaatcgcta aatcatctaa atggacaagc tggtctttga
    36481 tcgcgcctgt cagcgctagt tttttgccga tttcttcatc ttcaaatttg ggccataaaa
    36541 tccctggtgt gtctaacaat tccaactcat tacctaaacg aagccactgc tgaccttttg
    36601 ttacgccagg cttatttcct gtctgtgcaa ttttcttgcc tactagtcga ttcatcaacg
    36661 tagatttccc aacatttgga atacctaaga ccattgcccg aatagcgcga ggcttgatcc
    36721 cccgagcagc atctcttgcc cgtttttctt tcaatgcttc tttagccgca ggtactagac
    36781 ctttaattcc tttactttct tgtgccgtga tagctaatgc aagatggcct tgtttacgaa
    36841 aatattcttg ccacagcttt gtctgttgtg gatctgccag atccgcttta ttcaataaaa
    36901 caaggcgagg tttttgctga atgatctgat ctagcattgg attacgagaa gataaaggta
    36961 atcgtgcgtc taccaattca aatacgatgt ccacatattt cattttttca gaaacttctc
    37021 gtcgggcttt tgccatatgt cccggaaacc attggatggt cattgttctc tccattcctt
    37081 tcataccgga atattccccg gctttacttg tttttttctt tttcaatata aattcacttt
    37141 tttattataa agaaattcca tctattagtt aagtgaaagt gaaccagtcc tcattctatc
    37201 acatcttcac ttttttgatg atgcaaattt ttattttggt ttctatatat gaatctaaat
    37261 ttttgacagc gtttcctaaa taagagatga tagacgaaca aaacagaaag gagacgaaac
    37321 aaatgaatag tatggttttt gttaattttc ctgttgctga tgtcaatcgt tccgttgctt
    37381 tttatgaaaa gctgggtttt aaaaaaaatg aagaattttc tactgaagaa gctggttcaa
    37441 tggtatggga tgacactttt tggataatgc ttctctctca tgatttttat aaaaaattta
    37501 taaaagataa agaaatagct gataataaaa aaacaagtgg tgctttgatt tcttttagtg
    37561 ttgaaaatgc ggatgaagta aaaaaatttg ctcaaattgc aaaagaaaat ggtggtactt
    37621 tccacgaagt agatatggga atgcctgaag aagaaatgtt ttcattagaa gtccaagatc
    37681 tagacggaaa tacattagaa cccgtatgga tgaagatgta agcagataac taatctgacg
    37741 ttttttataa caaaaaaaca aaggcggatg atacgataag tattctccgc ctctattttt
    37801 tttattcgta caatctcttt tttaca
//
LOCUS       DACKAC010000021        37133 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.021, whole genome shotgun sequence.
ACCESSION   DACKAC010000021 DACKAC010000000
VERSION     DACKAC010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.021"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(135..2288)
                     /locus_tag="I0B46_001119"
     CDS             complement(135..2288)
                     /locus_tag="I0B46_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAR2083433.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDVVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(2582..3430)
                     /locus_tag="I0B46_001120"
     CDS             complement(2582..3430)
                     /locus_tag="I0B46_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAR2083434.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(3654..5594)
                     /gene="thrS"
                     /locus_tag="I0B46_001121"
     CDS             complement(3654..5594)
                     /gene="thrS"
                     /locus_tag="I0B46_001121"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAR2083435.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(5931..6110)
                     /locus_tag="I0B46_001122"
     CDS             complement(5931..6110)
                     /locus_tag="I0B46_001122"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAR2083436.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(6157..7980)
                     /gene="cadA"
                     /locus_tag="I0B46_001123"
     CDS             complement(6157..7980)
                     /gene="cadA"
                     /locus_tag="I0B46_001123"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAR2083437.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            8258..10537
                     /locus_tag="I0B46_001124"
     CDS             8258..10537
                     /locus_tag="I0B46_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAR2083438.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(10630..11277)
                     /locus_tag="I0B46_001125"
     CDS             complement(10630..11277)
                     /locus_tag="I0B46_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083439.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(11456..12175)
                     /locus_tag="I0B46_001126"
     CDS             complement(11456..12175)
                     /locus_tag="I0B46_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAR2083440.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(12306..13457)
                     /locus_tag="I0B46_001127"
     CDS             complement(12306..13457)
                     /locus_tag="I0B46_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAR2083441.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(13674..14414)
                     /locus_tag="I0B46_001128"
     CDS             complement(13674..14414)
                     /locus_tag="I0B46_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAR2083442.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(14418..14741)
                     /gene="rsfS"
                     /locus_tag="I0B46_001129"
     CDS             complement(14418..14741)
                     /gene="rsfS"
                     /locus_tag="I0B46_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAR2083443.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(14798..15391)
                     /locus_tag="I0B46_001130"
     CDS             complement(14798..15391)
                     /locus_tag="I0B46_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAR2083444.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(15375..16022)
                     /locus_tag="I0B46_001131"
     CDS             complement(15375..16022)
                     /locus_tag="I0B46_001131"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAR2083445.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(16035..16346)
                     /gene="yhbY"
                     /locus_tag="I0B46_001132"
     CDS             complement(16035..16346)
                     /gene="yhbY"
                     /locus_tag="I0B46_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAR2083446.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(16362..17471)
                     /gene="yqeH"
                     /locus_tag="I0B46_001133"
     CDS             complement(16362..17471)
                     /gene="yqeH"
                     /locus_tag="I0B46_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAR2083447.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(17468..17995)
                     /locus_tag="I0B46_001134"
     CDS             complement(17468..17995)
                     /locus_tag="I0B46_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAR2083448.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(18175..18960)
                     /locus_tag="I0B46_001135"
     CDS             complement(18175..18960)
                     /locus_tag="I0B46_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAR2083449.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(18953..19822)
                     /locus_tag="I0B46_001136"
     CDS             complement(18953..19822)
                     /locus_tag="I0B46_001136"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAR2083450.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(19823..21202)
                     /locus_tag="I0B46_001137"
     CDS             complement(19823..21202)
                     /locus_tag="I0B46_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAR2083451.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(21210..21629)
                     /gene="fabZ"
                     /locus_tag="I0B46_001138"
     CDS             complement(21210..21629)
                     /gene="fabZ"
                     /locus_tag="I0B46_001138"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAR2083452.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(21654..22130)
                     /gene="accB"
                     /locus_tag="I0B46_001139"
     CDS             complement(21654..22130)
                     /gene="accB"
                     /locus_tag="I0B46_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAR2083453.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVISEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(22133..23368)
                     /gene="fabF"
                     /locus_tag="I0B46_001140"
     CDS             complement(22133..23368)
                     /gene="fabF"
                     /locus_tag="I0B46_001140"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAR2083454.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(23387..24124)
                     /gene="fabG"
                     /locus_tag="I0B46_001141"
     CDS             complement(23387..24124)
                     /gene="fabG"
                     /locus_tag="I0B46_001141"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAR2083455.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(24136..25053)
                     /gene="fabD"
                     /locus_tag="I0B46_001142"
     CDS             complement(24136..25053)
                     /gene="fabD"
                     /locus_tag="I0B46_001142"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAR2083456.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEATPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(25160..25384)
                     /locus_tag="I0B46_001143"
     CDS             complement(25160..25384)
                     /locus_tag="I0B46_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAR2083457.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(25437..26399)
                     /locus_tag="I0B46_001144"
     CDS             complement(25437..26399)
                     /locus_tag="I0B46_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAR2083458.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(26409..26849)
                     /locus_tag="I0B46_001145"
     CDS             complement(26409..26849)
                     /locus_tag="I0B46_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAR2083459.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            27155..28120
                     /locus_tag="I0B46_001146"
     CDS             27155..28120
                     /locus_tag="I0B46_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAR2083460.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(28177..28407)
                     /locus_tag="I0B46_001147"
     CDS             complement(28177..28407)
                     /locus_tag="I0B46_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAR2083461.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(28493..29380)
                     /locus_tag="I0B46_001148"
     CDS             complement(28493..29380)
                     /locus_tag="I0B46_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAR2083462.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(29469..30692)
                     /locus_tag="I0B46_001149"
     CDS             complement(29469..30692)
                     /locus_tag="I0B46_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAR2083463.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(30709..31788)
                     /locus_tag="I0B46_001150"
     CDS             complement(30709..31788)
                     /locus_tag="I0B46_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAR2083464.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            complement(31919..33646)
                     /gene="ptsP"
                     /locus_tag="I0B46_001151"
     CDS             complement(31919..33646)
                     /gene="ptsP"
                     /locus_tag="I0B46_001151"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAR2083465.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(33646..33912)
                     /locus_tag="I0B46_001152"
     CDS             complement(33646..33912)
                     /locus_tag="I0B46_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAR2083466.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            34391..36625
                     /locus_tag="I0B46_001153"
     CDS             34391..36625
                     /locus_tag="I0B46_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAR2083467.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            36773..37093
                     /locus_tag="I0B46_001154"
     CDS             36773..37093
                     /locus_tag="I0B46_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAR2083468.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
BASE COUNT        10447 a         8033 c         6474 g        12179 t
ORIGIN      
        1 ttttttgccg agtaaacggt gttcaaaagc cagtttctcg gtatgaagtc aaatttaagc
       61 aaaaatgtga gaagctattt tcacaaaatt tttcttcata ctcgaaacta agcggctttt
      121 tcacgacctc ttttttattt tgccatgaac atatcataat acgcgtccat tatttcttta
      181 gcaatcgtca agtttatgtg gtcttgttcg tctaataagt tcggcaagac aacactgatg
      241 gcaatttccg gattgtctgt tggtccgtat gccacgatat tactattgac tgtcgcaata
      301 tctggatgac cttccgcaac cgtttctgca gtccctgttt ttgcggaaag gtccattttc
      361 gcactggcta atggtttggc tgttgtgtaa ggatcggtac catggacaac ttggtggaat
      421 ccttcacgga ggatcgccat attttctgca gaaatattga ccgtattttc tactgttgtg
      481 ccaatttctt tcttcacttc acccaagttt ccattttcgt cattgccata gatcgctttg
      541 actaggtgcg gctgtactcg tttcccgcca ttagcgactg ttgaagcata ttgagctaat
      601 tggattggtg tatatgtgtc aaactgtcca aatgccaaat cggtaaagtt acctggagca
      661 taccattctt tgccattatc cgcattgaac ttcttcatat aatcaacaga acgtgagatc
      721 ccaggagatt cattcggcaa atcgatccca gtcgttgtac caagaccaaa ctctttgaaa
      781 gagtctctca attgctgata agctttagct tcttcatcta aactaggtag tcccattcca
      841 ggtgtatagt ctagtcctaa catctttaac gccactttga tcatatacgt attagaagac
      901 aattccgatg ctttgactac atcaagagca acttgtccgc ttcgattaaa gacagagctt
      961 ttttctgaag caccttgcag acgaatcggc tcatcaatca aaacttgatt tccgctgatc
     1021 gcgttacttg cccagcctgc tgtcaaagta ccagccttga ctacagaccc tggatcaaag
     1081 gcgctagtaa tcgtgcctaa agcattctcg gtgatttctt tggaaccttg ttcatgactg
     1141 aaaccagtca ttgctagcac ttctcctgtc tgaggattca tagccactgc ataagcacca
     1201 ggcgaatact gtgctttgcc gttgttgatc aatgtttgat aattcctttt caaaatttct
     1261 tctacttttg attggaattc tgcattcata gataacatta aattagaacc tttttcccca
     1321 gagaagattt ctttttgatt cgaaacattt ccttcattat caagggaaac ttcatattgg
     1381 gttttggttc cttgcaggac atcttcatat tgtttttcca aataactttg tcctacacgg
     1441 tcattccgag aataaccttt tgctaggtat ttctctgctt catctgctgg aagtccttgc
     1501 ttttcagtcg tcacactgcc aagaatactt ttcagtgaac ttgctgcatt atattctcgt
     1561 gtccagtctg tgccagtcga aactcctgga agttcagaag cgcgttctgc cacaacagct
     1621 aactcttgat cagtaacacc agagtttttg atatagacag tgtttaattc atatgcacta
     1681 ttcatccgtt taaagatggt agcaattttt aattgctcct cgtttaaatt aatctcatca
     1741 gctgtgactt tattgaccat cgcactatat tgttcgctgt tccctaattg cttttctttt
     1801 gccgaaagtc tttttgtggc ttctttcaaa tgtttgggat cagccagcca aaaatctttt
     1861 ttatctctat ctgtcagatt ttcatctacc ggaacatcga tcagcgagtt cagcttcgtt
     1921 gcgatttcaa gcaaatcttt agccgtaact tgtgttcctc tcgtatatgt gatggcctga
     1981 ttcgctttat tcgtaactaa agcttttcct gtggagtcgt agatcattcc gcgaggagta
     2041 ctttcttgga ccgtaatcgt agaagtagac ttcactcgcg cctccatctc ttcgccgttc
     2101 acgatctgca aataacccaa tctagcaatc aacgcaacaa ataacgtaaa tataatgaaa
     2161 aaaagaaaat ttaagcgaaa aggaacatgg gaccggcgaa acgttttgtt cttaggttct
     2221 tgtgcacctt ttcttatttt ttctaaccaa gaagtattta atattttttt cataaaatct
     2281 ttcatcattt cctatccttc cgcctattta cattcatgct tcattgtacc caaaaacttc
     2341 ttaataaaaa agaaaaaatg ggcggagcaa gaaattcttt acctaatttc aagataaaat
     2401 caactttttg tttcacagca atcacttaaa gttacatatc caactatttt tttcattaaa
     2461 aaataaactt atttttgaaa attatgtttt cttaagtcgt gaaacacttt gatggatcat
     2521 ttatttgtgg aaaactacgt tttgttattc taaaaaactt atataaaaaa tagtaaggtt
     2581 tttatttcga atccataaaa tattcttgga ttgcttctcg tgtttttttc actttttcgc
     2641 taagcttttc gtttttagcc atatagaaat aaaaaatgat atcgatcgta gcaaattggg
     2701 ccaaaagtga attagttgcc gcacttcgat tcgtgatttc ttttggtcta gatgtttgta
     2761 cgagtacatc ggctatcttg ctaagcgggt tatttccaaa ttgcgtcaac gcaatgattt
     2821 cgatattcat gcttttcgcc agctcggcta atgcgactac atctgcactt ctacctgagt
     2881 ttgaaacaag ccaaagcact ttctttttct cattgctgac aagttgcggg agcaagacgt
     2941 ggatatcttt ttcaaaaatg attggcttac cgacacgaga ccatttttgc agaatatctt
     3001 ctactactaa agaagaggcg ccaaccccat ataaaaaaac agtctctgcc gattccaaac
     3061 actctacgac tttttggatc gtttcttctt ctagaatatc tactgtttct tttatcgcta
     3121 cttgtgcatt gttaccaagt ttgtttttga tagagtaaaa ggactcattt gcttctatat
     3181 cactaaaacc ttcatcgatt ccagtctgta gttctgttga aatcgtgatt ttgaagtccg
     3241 tcagactttg aaaaccgatt tttttggaaa aacgtacaac tgtcggcgca ctcaacccgg
     3301 aagcttctgc taattcatta gctgtcattg tagggacctt ctcaatatta ccgataatgt
     3361 agttggccaa ctttttttct gctgaagaat aatcttccat catactttca atacgactaa
     3421 ttacgctcat aagttcctcc acccttttaa tgatagtcta tgtaagtata ccatatcaga
     3481 cccttattca cggtcgtata ctggataaaa aacaaaaaaa caatccgccc aacagaatat
     3541 cttatcccat caatcccaca gattctattg agcatctgtg tctttgagga atacctgtta
     3601 tctattgttc ggattgcttt ctatctacta cttttttaca ggaaatttac ctattagttt
     3661 tctctgctgt aatttttgac ttctgcttcc atagcatcaa taaagatatt caaatcttct
     3721 acagctgttt ctttacttcc ataacgacgt acgttcactg ttgcatcttc caattccttg
     3781 tctccaacaa ctaattggta agggatcttt tgtgtttgag aggcacgtat cttatagccc
     3841 attttttcat tacgatcatc aacttctaca cgcaacccct tcatttgcaa acgttctttg
     3901 atttcataag cataatcgcc atgtgcatca acagaaacag ggatgattgt tgcttgtata
     3961 ggtgccaacc atgttggaaa agcgcctttg tatacttctg ttaaataagc gacaaaacgt
     4021 tccattgtag aaacaattcc gcggtggata actactggac gatgcgtatt ttccccgtct
     4081 tctccaacat atgtcaaatc aaagcgctct ggcaataaga agtctaattg tatagttgat
     4141 aatgtttctt ctgtaccaag agctgttttg acttgaacat ctagttttgg tccgtaaaag
     4201 gctgcctcgc cttctgcttc aaagtaatct agttctaatt catccatagc ggctttcagc
     4261 attgcttggg ctttttccca cattgcgtca tcatcaaaat atttatctgt attgtttgga
     4321 tcgcgatagc tcaagcggaa acgataatct gtaatattga agtcagcata tactgccacc
     4381 atcaattcaa gcgtacgttt aaattcatct ttgatttgat caggacgaac aaatgtatga
     4441 ccatcattca acgtcatttc tcgtacacgt tgtaaacctg acaaagcacc tgatttttca
     4501 tagcgatgca tcattcctaa ttcagcgata cgaattggta attcacgata agaatggatc
     4561 gtgtttttgt agaccatcat gtgatgaggg cagttcattg ggcgaagaac aagcatttcg
     4621 ccatctccca tatccattgg cgggaacatg tcttcttgat aatgatccca gtgtccagat
     4681 gttttgtaca actcaacatc tcccattatt ggtgtatata catgttggta acctagacta
     4741 acttctttgt ctacgatata acgttcgatc gtgcgtcgga ttgttgcccc ttttggaagc
     4801 cagaatggca aaccagaacc tacttctggg gaaaccatga acaagtcaag ttcttttcct
     4861 agtttacgat ggtctctttc ttttgcttct tcacggagac ggatatattc tttcaaagct
     4921 ttcttgtcaa aaaaggctgt cccgtataca cgttgcatca tttgattgtt tgagtttcca
     4981 cgccaataag cacctgccac tgataataat ttaaatactt gtatacggcc agtggatgga
     5041 acatgtggtc cacgacacaa atcgatgaat tcgccttgtt gataagcagt aatcacttct
     5101 tcttctggca attcactaat cagttctact ttgtacggat cacttgcaaa aatttctaat
     5161 gcttcctgtt ttgaaagaac acggcgttcg atcggcagat tttctttgac gattttcatc
     5221 atttctgctt cgattgccgg caaatcttct gcagttactt ggttttgtcc attatctgta
     5281 tcgtagtaaa accctgaatc gatagctggt ccaacgccaa agtggatatt tggatagagg
     5341 cgacgcatgg cttgggccat caaatgagct gctgaatgac ggactaagcc aagtgcatct
     5401 tcatggtctg gtgtgatgat ctcaatactt gcatcttcct cgattggacg agtcaaatca
     5461 acgagttttc cgttcacttt accagctaaa gctttttttg ctaagctttt tgaaatgctt
     5521 tcagcaattg ccaatgttgt tgttccagac tcaaattctt tgactgcacc atctggaaaa
     5581 gtaattttta tcataatagt ttcctccttg aaatgttttg attgatcaga tcatcaagct
     5641 gaaaaacaaa aaaatcctag catctcttga aaagagatac taggacgaaa agttcgtggt
     5701 tccacctaat gttcaaacgc aaatgcgttc ctcaagcgtt gtaacggtcc gaccgccctt
     5761 gcttcaacaa gggtttcgaa agtggtcatc ctttggtttt ctcctggaaa agtttcagcc
     5821 tagcttttcc tctcttatcg aagactctca aattcagttg gctttctcat caatcaatta
     5881 tgtgtttatt taaccactaa taaatagaaa aatcaagtgg gaaactaaac ttattttttc
     5941 cattccccat ttacaccgta tgttcctttt ttcatctcat tgatgatgac atggatgtgt
     6001 tcttttggtg cctgcgtgtt tttatgaaca gcttccgtga tatctttcat catattttct
     6061 aattgttccg gactgcgacc ttctactaat tcgacatgta cgaatggcat aatgctctct
     6121 cctctttctg atcgttcttt tttcaactcg attctatcat actatgtttg tcttgggtac
     6181 tgaatcagga aaaatggctg gatctttttt tgctctcaag gcactcaaaa tagagaccgt
     6241 atccacgact tcttgcaaaa cagctccgaa taatgcgggg attagtcccg tacttgcaat
     6301 taacatcaaa aaaatacaaa taaagatgcc aatcagtaca gactgtttcg ctattctcat
     6361 cgtatctctt gaaatcacta ctgcttttgc tacacgcaag agatcatctt tcaagatgac
     6421 cgcatctgca ctttcactag cagctgtaga accatgagcc cccattgcaa taccgatatc
     6481 tgcagcagca agtgctggtg cgtcattcac gccgtctcct accatcgcaa caggtctttg
     6541 atctttaggg atactttcta aaatggtcag tttatcttga ggcaagctac gggcatgtac
     6601 agaatctaat tggacttttt ctgcaatcgt ttgagcaatg gcagatccat ccccggtgat
     6661 catcatcaaa tttttcacat caagttttct tagctccttg attgtctttg ctgcttctgg
     6721 gcgaatctga tccgaaaata ggatcgttcc tgcaaaagat cgatcaattg ttacatagat
     6781 tgctgtattg gacgtttcta tttcttcagc tgaaacaaaa gaggcacgtc caatctttac
     6841 ttcatgatta ttgactactg cttggattcc ttgaccggtc acttctttca gttgactaac
     6901 aggatacagt tggctgtttt tcacgttgtt cactaacgaa cgagctaaga tatggctaga
     6961 ttcctgttct gcactagctg catacagaag tagctcttct ttttttattc cattgactgg
     7021 ctgaatcgcc tctacttcca atatcccttt ggtaagcgtt cctgttttat caaaagcaat
     7081 cgtttttgta cgtgcgagct tttcaatcgt tgttccagtt tttacaacga tcccgttttt
     7141 acttgaacgg ctcattcctg caactaaagc aatgggcgca gctagaatca gtggacaagg
     7201 tgatgccact accagtacct cagcaaatcg taaagggtct tttgagaacc accaagaaat
     7261 accaccgatc aaataagcaa tcaacgtgaa agggactgca taacgatcag ctaaacggac
     7321 aaatcgggca ggcttttctt ttgattcttc gactagttta ataatcattt gatactggct
     7381 gtctgcggca cgtttttcag cgatcatttg gatcgatccg tctccattta aagaccctga
     7441 cataatctga tctccaggtt tcttggtttc cgggcgggac tcacctgtca aagaagattc
     7501 atcgaccgaa gattctcctt tggtcactcg accatctgcg ggtacaagtt cgccaggctt
     7561 gactagtaac tggtcatcaa tatttatttc atcgattact atatcctgca attttccttc
     7621 tttgaatcga tgggcaattc ttggggtgtt atctaacaaa gactgcagtt ctttacccgc
     7681 tcgtttattc gcgtaatcct ctagactatc tccgcccgta agcatgatca atatcatcaa
     7741 actagcccag tattctttga cggctaatgt tgcaaggatt gcggtaatcg ctaatacatc
     7801 tacgccatat cttcctgaac gtaacgtctt gatcatttcc aaaaacatca agaaggcgag
     7861 aatccctcca gtaaatgcga caatcgcaaa tgcaagggcc ggctggtgaa aaacaaattc
     7921 acttaataaa gctatcccac caataagcaa agtaagtaaa aattttttga aatgtgtcat
     7981 tgttttttct cctcttttat gtgacttaga gaggttagta ataaatttcc agctgcctct
     8041 ttcatcttaa gcatagtcct tgactagcac tttttaaaac aataggcctt aattgaaaac
     8101 aactatcact atcagcgttt ccaaaattca tcagttctac aaacaaaaat attcagcaag
     8161 gattactttt tcgattcatc agttaaaaag tacctttttt cgaaaaacta tgctatactt
     8221 ttaattcgtt atttacggcg gaaggagcaa acatgcgatg aatgagaaac aactagaaca
     8281 aacgcacgtg gatgaaacga tcgaactaat ccagatcgaa caaaatatgc tggatacaaa
     8341 acaacaagaa ttgactcagg aaatgaatca ttcgacaaaa gacacagcta atcacaagat
     8401 tcgagctggt tccaatgaag ctttttatga atctgctgtt gaatatcgcc aacatgagca
     8461 ggaattgctt ttgaaatacc atactcttga agcacagcaa aaacgcctgc aaacgttgtc
     8521 tgtcatgaaa gatagtcctt attttgcccg tatcgacttt aaagaagacc gtgaaacaga
     8581 aacattatat ctaggtatcg cttctttaag agatctggca gaagaaacaa tcgttattga
     8641 ctggcgcgca ccaattgcca atctttatta tgaaggtgag attggtcccg cattttatga
     8701 aactgaaaca gatagaatag atgtcgatct gcagttgaaa cgacaattta aaatccaaga
     8761 aggacgtatt ttatctatgg tagatacgtc ggaagtaatc aatgatgact ttttgctaga
     8821 gatattggat gaatcatcaa gcaaccatat gaaaaacatc gtctctacga tacaaaaagc
     8881 acaaaatgca atcattcggg acacaacaag taaagtaatg ctgattgaag gaatcgccgg
     8941 aagtggaaag acatccgctt tgcttcaacg tattgctttt ctgttgtatc ataatcgcaa
     9001 gtggctcgag gctgaaaacg ttcttctatt ttcacccaac catttgtttt ctgattacat
     9061 ctcgactgta ttgccttcat taggtgaaag cggcgtgcct acacagacat tcaaaaatta
     9121 tttggtgcag ctgctgccag agttttcttt actagatgaa cagcaacaag aaagtggttt
     9181 tttgctaggg gaaaaagatc cgatacaagt gatgaaaagc ggattgacct tagtcgatca
     9241 gattgatcgc tatatccaag gcatcacaaa ctacggtccc ttgttccgtg atatgaaaat
     9301 aaatggccgt acgatcctat caaaggaatc gattcgccaa tggtataaag aaacaaatga
     9361 acaattacct ttacatcata gactttctct attacaaacg aagctcctga aaaaattagg
     9421 cggtctgcaa aaagacgaaa cacgtcagca atgggtcaaa gatctggcgg aagaacagct
     9481 ccaagaacta tacgcaacag atccaaatct tgaatataca gaacaaaagg agcgttcatt
     9541 acgtaaaaaa ctggccaacc aaatcgtacg taaaagattc cgtcgaatcc atcgagggat
     9601 catgaattat caatttgtca atataacgaa acaatatctc cactttctac aaactgtgcc
     9661 actttctctt ctaacacgac ataaaatcag cgagaccagg tggcaggaac atttgacaac
     9721 tgttcgtaca cacttgcgtc aaagagaatt gcgtcaagag gatgctgtac tattcttttt
     9781 actgatgcgc cgaacatatc cagtgacggt tacacacaaa gctcgctata tctttattga
     9841 tgaaatgcag gatttcgcac catcacaagt ggcattgctg caagccttgt tcccaaaagc
     9901 aaatttgact ttctgtggag atctaaatca aaatgtcttt ggaaatgaaa cgatcgttgg
     9961 atcattggaa gaccttttcg tggaccgtga agtcactcgt ttccaattaa cgacaagcta
    10021 tcgctccaca aaagaaatca ctgattttgc taatcatttt attgtgaaag aacatcaagt
    10081 agaaacaaca gcaagaaaag gagaccttcc tttgctcatc caaggaacaa cagatgatat
    10141 caaattgaat tggttagcag ataccattcg aacgactcag ggaaaagccc ggtattggcg
    10201 tacagcaatc atagctaaaa cacaagaaga atgcgaagct ctttatgctc aactgccaga
    10261 cgagttgaaa caacaagtcc agctgatttt aaatgagagt gactttatga agcgttccat
    10321 tattttactt ccagcctttt tagcaaaagg tctggagttt gaccgagttt tcttgtggga
    10381 tgtagatgca gaacattttt cttctcagca agacaaattg atcctttaca ccatgtgtac
    10441 acgtgcgatg catgaattga ccctttttac ctccaataat gaaagtcctt tgttaaacaa
    10501 tatggatatt tcatcttatg agaaaaaagt actgtaagta aaaagacaac tgctatcatt
    10561 aatgttagca gttgtctttt tatttacaat aataattgca gaaaaagtaa ttcaatcatt
    10621 taaggtcatt tattctatat ataaatccaa atctgtttca ggatatttta atttgacaag
    10681 cgaaataatc attttcagtt cttccatgtt ttctggatat tctgggtcaa ataccttgct
    10741 atgacggcca atcccgccat tgtcgatcaa tcggtaaatt cgattccatt caatgaattg
    10801 gtcaattgat tggaaattgc tgattggata gccgccacaa tccaaaatct ctgatacacg
    10861 aaatgctagg tatgcactat cgatcacttt attttttttc gtgtctatct tctcatttct
    10921 ttcgataaga attttttcag gacgatgttt caagctccac tctaatagta gaaagaaagc
    10981 ctccatccga tactcttctt caaataaccg aaaggctgaa tcagaacgac gaaaaagcag
    11041 cggaatcaga taattttgtc tataaatcag cattggtgcg cggtccacca tacactctaa
    11101 ataacggttc agataccctg taccgacata gtttcctaat cgatagcctt caacgccgaa
    11161 tcttgcgtat ttttgcgcta attttgaaca atcagttacg ttcacagcta actcttctac
    11221 tttgcggtaa ccgcttaaga tacgttcaaa aggttctaaa gtgatttttg cttccatatt
    11281 cttccccgtc catcttccac atacttcata tcatttcaca tgatacttat gctctcatca
    11341 taccacaggt tgaattagaa aagaaaataa atcacacaaa aaagcaagac aatctgttag
    11401 tgagattgtc ttgcttttcg aaaaagactg aatacttttc gttaaaactg gttgcttagt
    11461 cttctaatgc cacattatgg aacacatgct gtacatcttc tagatcttcc agtgcatcaa
    11521 tcattttttc aaatgtttct tgatcttctg gagatagtgt tacctcgttt tgagggatca
    11581 tttggatctc agcgattgaa aaatcagtga ttcctttttc ttttaatgct tcttgtatac
    11641 tatggaaatc cgctacttca ccgtaaacga tgatttgtcc ttcttcttcc attacatcgc
    11701 gaacatcgat gtctttttcc attaggtatt caaggatctc gtcagcgtct tctcctgcaa
    11761 aagcaaagat tgctgtattg tcaaacatgt aagaaaccgc tccggaaaca cccatgttcc
    11821 cgccattttt cccaaatgct gcccgtacgt ctgctgctgt tcgattcaca ttgttcgtca
    11881 atgtatcaac aatcaccatt gagccgcttg gtccaaatcc ttcatagcgc aattcagagt
    11941 atgtttcatc tcctgcacct tttgcttttt cgattgctcg gtcaataata tgttttggta
    12001 cattataagt tttagcgcga tcaatgacga aacgtagttt ttggtttgca tgcggatcag
    12061 gatcacctga ttttgctgcc gcgtagattt caatcccaaa ttttgcataa atccggctat
    12121 tattagcatc tttagcagtt ttctttgcta cgatatttgc ccatttacgt cccatatttt
    12181 cacttcgctt tctgatttga tagtttcata acgcaaaaac gtcctgttcc attatacttg
    12241 tgaatcacga ttctgtgaag ctttttttct ttcttccttc attcttctca tttttagtca
    12301 attatctata ctcggattgg gatttgcccg aaattctgtt ccggaatatt gcctcccaat
    12361 tgatagattt gatcacttct aactaacagt tccgcttctt gtgcctcttt ttttccgatt
    12421 cgggaataaa tagaaatctc ttttttttct tttaagaatc ttcttccagc atcggtaaat
    12481 ccaagtagtc gaagataatt tttattttgt tggacaggaa tctcttgctt tttgatattc
    12541 agcaagacat aacaagataa tcgttggatc cgtgtccatg tatatctttt tgtttttacc
    12601 tttgacacaa aatcctcaaa actatcagca gttgcagctg ctgttttcat gcgcgattct
    12661 attccttcgg tcatctgata gatatctgtc agttccagca aagtagaaga taacaaccga
    12721 taacgcaaat acggccaata agattcccaa gtctgcagcg aacaagttga aagatcttct
    12781 tctgtctgag gcggcacaag tccagctatt ctttttcctt cttctaacgc atggcgaatg
    12841 gctgttgcac tggctatcct ctgatctgtg atgatttttt catgatagcc tgcactttta
    12901 cgtcggatcg gatgtaattc catagggtgg acatacttgg cattttcctt tgcataactt
    12961 aatcccaaga tatgatttgg cgaagaaaaa tcaagcgtaa tatcgggata cagactgcga
    13021 aaaacctcag tcattttttg cggataacta tacgtttgat cagacagttc ttgaaatgcc
    13081 tcttctatcc tatcttgatt ctcttggaca aagcgtccga aactgctgta atcaaacgaa
    13141 gtctcttctt ctgttccaaa acacaaactg tcacagtcca gtgtttgcaa gatttttact
    13201 gcacctttag caaaatagtc agctgactga accgcccatt cgacgggaag ctccactact
    13261 aaatccacac cattttgtaa agcttcattc gcgcgatgcc atttatcgat gatagccggt
    13321 tcgcctcttt gcaaaaaatt cccgctcatt acagcaatga cgacctctgc tcctgttatt
    13381 tttcgtgcca tttccacatg atagcgatgt ccattgtgaa aaggattgta ctctacgata
    13441 atcccgcagc ttttcatgcg catcctcctt catagttatc gataaaaaag aaatagagac
    13501 aagtaaaaaa aggcaaaaat cggaaaaaat acccgaacta tataaactat tctcgcaaac
    13561 acaatacttc tgcgacgaaa agctgcactg caagagcaat gcctatatcg ttcggatata
    13621 taatttatgt tgaagattta ttcttttctt gttaactcga gcaggcaaaa ccgctagtct
    13681 ttgtatgcta caaagaacca gcgctttgat ttctctgatg ctgcttcgtc tgtaaaatca
    13741 gaaaatgctt cgactttcga aaagccgctg ttttccagca ttcttaaata actttccaat
    13801 ggataggtac gctcttgatg tagttcatct tcacgaataa atcgaccaga cccttcttgt
    13861 tcttcaacga aaaaggtaag aaaatgttcg atgctgtgtt tctcgcttcc tggataacta
    13921 tcccacaaaa aagcaaaatt atccccttga taatgatagc tatattctgg aaaaacctca
    13981 tctacttgat aggtggagtg tacgtcaaat aaaaatcggc cattttcttc taacgcttga
    14041 tagacttcat caaacacttg ctgtacctct tgtctatttt tcatatagca cagcgaatca
    14101 gaaaaacagg taattgcctg atattgtccg atttctgata aatcgagcat attgccttcc
    14161 acaaattgga tcgatgcgtc ttcttcatat gctcgctggc tggcaatcat caacatctct
    14221 tctgaaaggt ctaaccctgt cactttatat ccatcttttg caaaggcaac cgctaatgct
    14281 ccagtaccgc atgccagttc caaaatattt tttgtgtctt cttgtaaatg acgtttcgaa
    14341 aaagctaacc attgctgata aagtgtttcg tccatcactt catcgtaaat gaaagcaaat
    14401 gtttcataag ccatatgtta atccaaccat tcactaagat cgactagcgg agcatctgac
    14461 catagttttt ccagattata gaaatttcgt tctggcgcat ggaaaacatg tacgatcaca
    14521 tctccaagat cgatcaatac ccattttccg ccttcttttc cttcaatacg ttttacttca
    14581 tatttgtttt cttcttcttt atcaatgatt tcttctgtaa ttgcgttgat ttgacgttcg
    14641 ctatttgctg aacagatcat gaaataatct gccaacaagg aaacatttcg aacgtctaaa
    14701 gcgataatgt cttctgcccg ttttgaatct gctgcttcaa tcgcaatttt taattgttct
    14761 ttactatcta tgatgatttc ctcctaatat gagcagctta tttttttgct acccagtaat
    14821 tatatgtttc gattgttttt ggataaatct gttgttcttg cgcaagcaaa tgggccaatg
    14881 tatgttttgt ttcaaatgct acagcttcgt ctagatcagc ccatgcaatg gctcgtgcat
    14941 cttgtacccc aggaaaatct cgacctggtt caatatagtc tgctacataa atgattttgt
    15001 caaggagact catcttagca gccccagtcg tatgcacccg gattgcatga agaatctctg
    15061 catcggtgat tcctaattcc cgctctacaa acgaagcgcc aacaagacca tgccagattg
    15121 cattattata attcaataac tccggatcaa aaccgtctct tacgattacc attttgaact
    15181 cctcatctgg tcgttctttt gcataatcat gtgtcaatgc agcaatacta gctttttcag
    15241 gagaagcacc gtattttttg gcaagagcca cggctgtttc ttctactcca agcacatgct
    15301 tgaagcgttg ttcgctcatt ctcatctgta ttttttgcat caggttttct ctagaataag
    15361 cagtatattc attactatag tccatattca tacaaccctt cattttgaat ataatcaatt
    15421 actttatctg gaacaaggta gcggatagaa cagccttctt tgatcttttg acgtattttc
    15481 gtggaactga tgtcgatctc aggtacatct acccaaatta caggatatgg agtatccgtt
    15541 gtgtatcctg gtcgacgtat acccacaaag ttgaccatag aagtcagctc atcgatttta
    15601 taccattttg gcaaatattc taccatatcc ccaccgataa taaagtaata atctgtatcc
    15661 ggattgtttt gcgtcaattc cttcatagta tcataggtgt aacttttccc accacgagct
    15721 aattcaatcg tttcaatctg caaaaaagga ttgtcttcca ccgctaattc caacatgttg
    15781 agtcgatgtt ttgcatcaat cgtctgtttc tcatcaacat gaggtggctg ataagaaggc
    15841 atcaaaaata cgcggtcaag acctaaattc cggccagctt gttcagccat cactaaatgt
    15901 gcaaggtgaa cgggattgaa tgttccgccc aagatcccta cttgcttttt cttctctaag
    15961 aattgaggca tttcttcagg aaataattgt gaacgaacaa atgtcttagc ttgcgtattc
    16021 atcatggtca ctccttatat tgcttttact tcttttgata gcctttgata cttttctttt
    16081 gtggaaggtt tgtataatac aataacacgt ccaatgattt ggacgatttc gcaatctatt
    16141 tctttttcta atacttgagc tgcttcttct gcaatctcat ctgtattttg aagtaacgat
    16201 actttgatca gttctctttt ttctaaagct tcttcaattt gaacgatcat tgctgaattg
    16261 atcccacctt ttccgatttg aaaaatcggc tgaagatgat gcgctttgct acgtaaaaaa
    16321 cgcttttgtt ttcctcttaa actcataatt ttccttcttc cttaaatcaa tgctttgcgt
    16381 ttcaatacat ctacaccctt tggcgcccag cccgcaatta ctactggcgc tgttacagtg
    16441 atccagccta gaccggcaaa aacgatgtct gttttttctt tcacagaaaa ctcaaaacga
    16501 acaagctccg gaaatgcctc gacttcatca ggacgaggag gctgtaataa tccacctaca
    16561 tgtttttgat aaaattcgtc tgctttttcc agtttcgttc gatgaatatt tacatcattt
    16621 gacacataag caataaatga agcacgattg ccgctgataa aatcaaatct tgcaagtccg
    16681 cccaagaaaa gtgtttgccc ttcattcagc tgataaactt taggtttgat ttcttttaac
    16741 ggtgcaatga tccgcaaatc ttttttacct aagtagtgcg ccatttgatg gcgatggata
    16801 attcccggtg tatcaatcaa aaaatgcccg tcatctaaag gaatctcgat tttatccaag
    16861 gtcgttcctg gaaactgcga agttgtgatt acatcttgta ctcccgctgt atttttgatg
    16921 atttgattga taagcgtcga ttttccgaca tttgttactc cgacgatata gacatcccgt
    16981 ccatcgcggt attcttcgat tttttctaat agttccgtca tctcatgaga tttcttagca
    17041 ctagttaata atacatctac tggacgaatt cctgcttcgt aagcacgttc cttcatccat
    17101 tgtgttaatt tagttttttt caatgattta ggtaatatat ccactttgtt cccaactaaa
    17161 agtactggat tgtcaccgac aaaacggtgt agtcctggga ttagtgaacc attgaagtca
    17221 aaaatatcca ccacattgac gatcaatgca tctttttgcc ctaattcatt cagtaagcgc
    17281 aaaaagtcgt catccgtcaa tgagacatct tggatctcat tataatggcg taatctgaaa
    17341 caacgctgac aatagacttc cccggaagct aaaccttttt ccaaagccgt ttgcggcgtg
    17401 tatcctaatt caccgggttt gtttgtttgt ataacggcac cacagccgat acattgtaat
    17461 tcttctgtca ttcgattcca cctcgccagc ccatatcagg gtgttttgct aatagatagg
    17521 ccataattct tttttcccaa aaacgattaa attgtgtctt ccaactatct gttgcgataa
    17581 tgggttttac aaggatgcta cgtatccctg cacgattggc tccacgtata tcagtcataa
    17641 tctgatcgcc taccattact atctcatcat ctgctaaatc caattgtttc tttgctatgt
    17701 tgatccctct tgctaaaggt ttcattgctc gtgcaacata caaaagatca aatttttcaa
    17761 tcgctcgcgc gacacgtttt gaattattgt ttgaaacaac aacaacaggg attcctgcat
    17821 ttttcatttc taaaatccaa gtaagcaact cttctgttcc atctggatta ttccatgcaa
    17881 tcaacgtatt gtctaaatcg gtcaatactg ctttgatgcc tagattcttt aattgggcag
    17941 gtgtaatctg atagatggct tctaccatcc atgttggttt gtaatttgaa aacatgctgt
    18001 ctcctcatct gcaaaaaatg aaggagcaca taaagtactc ctcaaccccg tattatactg
    18061 catatcagca aaaaaaacta cctctatact cgttcgtctt tcagaaaaag catttcttta
    18121 caaaatgacc aaaaataaag attgatcaca gaaaaattta gaaacaaaaa acaactaata
    18181 ttggcggaac ctcttgtatc gtttttccag taatttatgc tgatctaatc gaacaagttc
    18241 cgtcaatctt tcaatcaacg cttttttcat aatgtgtgcg actttatatc gtggcaactg
    18301 ctcgccgttg aacgtttcag gaataactcg atcaacgatt cctaactcgc gtagttctgg
    18361 cgcagtaatt ttcatcaact cagctgcttc tgcggcgcga ctaccgtctt tccacaaaat
    18421 cgacgcaaat ccttctggag aaagcacagc ataaatcgtt tcttccagca tccagacttc
    18481 atccgctaaa cctagagcaa gggcaccgcc acttccacct tcaccaatga tgatggcgat
    18541 gatcggtacc tttaaatcag acatttcgag cagatttctg gcaatagcct ctccttcgcc
    18601 gcgctcttct gctcctacac cgcaatatgc acctgctgta ttgatcaagg taatcaccgg
    18661 tctattgaac ttttccgctt gtttcatcag tcgtaaagct tttcgatagc cttcaggatg
    18721 gggagaacca aaatttctgc ggatattttc ctgcagatcc cgtccttttt gaataccgat
    18781 cactgttacc ggtttgccat ctaatcgagc gattccacca acaatcgctt cgtcatctgc
    18841 aaataaacga tcaccatggc attcctcgaa agaatcgaag atctcttcaa aaaaatccaa
    18901 gcttgtccat cgttctttag ctcttgctaa ctgaacgaca tcatgtgctg tcttatccac
    18961 gatctatcca tcctttctgt gtatgcaatt caagaagcgt atggattttc tgtttcaatt
    19021 ccatgcgggg aacaatctga tcgacaaaac catgagataa taagaattca gccttttgga
    19081 aatcttctgg aagttcctgc ttgatcgtct gttcgatgac gcgtcttcca gcaaaaccga
    19141 tcaagctttg gggttctgct agaataatat cgccttccat tgcaaaactg gcagttactc
    19201 ctccggtcgt cgggtccgtt aaaacagtta gataaaaaag accttcattg ctatgccgtt
    19261 tgacagcagc agagattttt gccatctgca ttagagaaaa gattccttct tgcatccgtg
    19321 cgccacctga agctgtgaac aagacgactg gaagactttc ctttgttgct cgttcgaata
    19381 atctggtgat cttttctccg acaatcgttc ccatacttcc catgataaaa ttaggatcca
    19441 tcactccaat aatgaaggga atcccctgaa tggttgcttt accagtgagg actgcttcat
    19501 gaagtcctgt tttatcttgc atttttttaa ttttttccgt atattcggga aatgctagcg
    19561 gatctttggt tacaagatcc gtatcccatt cttcaaaact tttttcatca ataatcaaag
    19621 ctagacgctg ccatgcaccg attcgaaaag cgtatccgca atgagggcat actttttctt
    19681 caccgatgtc tttcgtataa aggatatgtt tgcaatttgg acactttgcc cacatgttat
    19741 caggaacaga tggcgagtta gcaggtttgc tctctctatt aggattgatc cgaatatatt
    19801 tctttttctt aaacagagcc atctatgcgc ctcctatctc cgtttctggt gtccaattcg
    19861 gtaaaaattc ttcttgtaaa aatgctgtgc tgtaatcacc tgctatgact gcaggatgac
    19921 taatcaaatc catctgaaat tctgcgttcg tgatgattcc ttctgtgata aattcactta
    19981 aggctcgctg catcttcatt aatgcttcaa aacgcgtatt tccatggacg attatttttg
    20041 caatcattga atcataatat ggcgggatcg tatatcccgg gtaaactgca ctgtctactc
    20101 gcagtcccat cccacctgct ggaagcagta agttttggat cttccctgga gaaggagcaa
    20161 aatggaaagc aggattttct gcattaatcc ggcattcgat tgcgtgtcct tggaatacta
    20221 cttcttcttg acacacattc aaaggctcac cggctgctat ctccacctgt ttttttacta
    20281 aatcgattcc tgtgaccatt tctgttactg gatgttccac ttggattcga gtattcattt
    20341 ccatgaaata aaaatcgcct tcttcatcca tcagaaactc gatcgttccg gcattttcat
    20401 aattgactgc tttagctgct cgaacagcag cgtctcctag tgcttctctt tttgtttgtg
    20461 agatcactac agaaggagat tcttccagta ctttttgatt gttgcgttgt aaagaacaat
    20521 ctcgctcgcc taaatgaatg acatgaccaa atcgatcacc taagatctga acttctatat
    20581 ggcgagcagg ataaatgatt ttttccaaat acatatcatc atttccaaat gccgctgctg
    20641 cttcttgctg agctgaagaa aaatgctgtg ggagctcttc ttttgattga actttccgaa
    20701 tgcctttccc gccaccacct gcagctgctt tcagcatcac tggataacca attctgtctg
    20761 ctatttccaa tgcttcttct actgtagata atacaccagt acttcctgga atgactggga
    20821 catttgcttc ttgcatcaat tgacgcgcat tgatcttatt tcccattgca tcgatcgttg
    20881 cacttttagg accaataaat gtgatattgc actcttcaca catggaggca aatcggctat
    20941 tttcagataa aaaaccaaat cctggatgaa ttgcttcggc ttttgtcacg attgcagcgc
    21001 tcagtacttc ctgaacattc agatatgaat caacggcttt tgccgggcca atgcagattg
    21061 cttcatctgc taactgtgta tgaagtgctt cggcatctgc ttctgaatat atcgctacgg
    21121 tttggatccc tagttcgcgg catgcacgaa tgatacgtac agcgatttca ccgcggttag
    21181 ctatcaatac tttagaaaac atatttcacc tatccaatca taaaggttaa ttccgcttcg
    21241 gctacttttt taccgtctac tttagcgatt cctttgccta ttccggctga agctttcact
    21301 ttcaatatct caacttcaag gtataaggta tctcccggtg taactttctt acggaatttt
    21361 gctttgtcca atccgccgaa ataagccgtc tttcccttga attgttcaag agaaagtaat
    21421 gcaactgctc ctgcttgagc cattgcttca acaatcaata cccctggcat aacaggttct
    21481 tctggaaaat gtccttgaaa aaagggctca ttgatcgtta catttttctt agcaacgatc
    21541 cgttctcctt caaccatttc ttccactcga tcaatcaaaa gcattggata acgatgtgga
    21601 atgatttctt ttatttcttg tatgttcatt gttttccctc tttcctgctt tgcttatttt
    21661 tgctgaatcc taaagagtgg ctgtccatat tcgacaactg actcgttttc tacaaggatt
    21721 tctgtgatca ctccgtcggc ctcactagtt atctcattca tcagtttcat cgcttcaata
    21781 atgcagataa cttctccttt ggcaacttga tcacctactt ttttgaaagc tggtttgtcg
    21841 ggcgctggtt gcagataaac gattcccacg attggcgaaa caacctcgat tccatcaaat
    21901 actgccgatt ctttttcttt cattatttct ggagcagttg ttgcttgttc agatatgaca
    21961 ttgactggct ccgaaactgg caacgtttcg tttgtttgaa ccatattcat gcctcgactt
    22021 gttttatttt tattcatata caattcaaat tgaccttcac gcaaatcgaa ttccgttagc
    22081 gaggattgat caaattgact gactaattct ctgatttcat tgatatccaa aattattcct
    22141 cccagcgttt catgcacaga accgcattat gtccgccaaa ccctaatgaa ttactcattg
    22201 cataagtaaa acttgcaggt ttgctttcgt ttttgacaac tgtcaagtca atctcaggat
    22261 cttgtttttc tacattgatc gtaggtggga taaattgatg ttctaatgcc agcaaagttg
    22321 ccaaggcttc gatcccgccg gctgctccca ataaatgtcc agtcatcgat ttcgtgctgc
    22381 tgacgtaagt atctctgaaa ttttcaccta atgcgatttg gattgctttt gcttctgcct
    22441 ggtcattggc tggtgtactt gttccgtgag cattgatata tccgacttgt tccggctgta
    22501 caccagcttc ttccatcgca agcttcattg ctttagatgc accagaacca tctggagtag
    22561 gagctgtcat atggtaagcg tcacagtttg caccgtatcc aacgatttct ccatagattt
    22621 tggcaccgcg agcttgtgca tgttctaaag attcaagaac tagaacgcct gctccttcac
    22681 ccatcacaaa accattccga ttttcgtcaa acggaatcga accacgatta ggatcagttg
    22741 attctgagag tgctgttaaa gctgcaaatc cagcaatccc catctcacaa atcgttgctt
    22801 ctgttcctcc agcaagaatg acatcagaat agccatgttt gatgttacgg aatgcttccc
    22861 caattgaatt cgttccgcta gcacaagctg ttacgatagc tgtacaaata ccttttgcac
    22921 ctacacgcaa agcaatattt ccagctgcca tgttggcaat cgccattggt acgaacattg
    22981 gagaaactcg ttttgcacct ttttcatgta gacgaataac ttggttttcg attgttggaa
    23041 gaccaccgat cccagatccg atgatgacgc caaaccggtc ttgattcatt ttttctttat
    23101 ctaaaccgct catctccatt gcttctaaag cagcgtagat tccatagatc gaaaacatat
    23161 ccattcgttt gctatctttt ttaataaaat atttatcgta agggaaatct ttgacttcac
    23221 ccgctaaggt aatccctgtt tctgaagcat caaatttagt gatcggtgcg atgccattcg
    23281 tcccctcttt taagctattc aaaaagctgt ctgcgtcatt tccaataggt gaagtgactc
    23341 catagccagt aattaccact cgattcattt gtttgctctc cttttcttat ccgtgcatga
    23401 ccaatccgcc atcgacattg atgacttgtc cggtgatata tggattttgt gctaagaaaa
    23461 ctgctgtttg tgcaatatct tctacttgtc cgaaacgctg catcggaatt tgcttttccg
    23521 cttgttcctt gactttgtct gctaaaactt ccgtcatatc agttgtgatg aatcctggcg
    23581 caatggcatt acatgtaatg cctctagttg cagcttctcg tgccacagat tttgttaatc
    23641 ctactacgcc agctttacta gctgcataat ttgcttgacc aatatttcca atcaacccag
    23701 atacactaga gagattaatg atggcccctt ctcgctgctt catcattttt ttcaatacgt
    23761 gctgcgtcat attgaaggtt ccaatcaaat tgatatccag acattttttg aaatcttctg
    23821 catccattcg catcacaagt ttgtcatttg tgattccggc attgtttact aacacatgga
    23881 tcgatccaag cttttcttct gcttctttga tcatttgtcc cgctttctca taatcagaga
    23941 tgtcaccgga aactcctacg cattttacac cgaatgcttc gatttcttct atcttttctt
    24001 ttggaatctc tccgcgacca ttcaaaatga tatttgctcc agcttttgca aaagctaagg
    24061 ccatcgcttt gccgattcct cttgtactac ctgtgataaa tacatttttt ccttttacat
    24121 ccataacgtt cctccttata gtattgctat tgtttcatca aatgtctgct tattctcaac
    24181 acgcagaact ggtattgttt tatcgatttt tttcataaat ccgcttaata cttttccggg
    24241 gccgacttcg atgacttgtt cgatgccgat tgtttttaac ttatggatac tctcatagaa
    24301 acgaacaggt tgcatgactt gctgttcaag tagtcccgcg attgtttctt tcttcattat
    24361 ttccgttgtt gtgttgctta tgataggaaa agaaggttca gagaattgaa tctgacttag
    24421 gtccttcgcc agtttttttg ctgccggttc taaaatagct gtatgaaaag gcccgctgac
    24481 atttagaggg atcattcttt tgaacccttt ttcttttaat agtaaaacag cttcgtctac
    24541 tgctttttct tcgccaccaa tcacgatctg ctgaggtgta ttataatttg caggagatac
    24601 gatgccgtac ttgctagctt catgacagct ttcctcgatc gtttcaatcg gcgcattcat
    24661 cactgcaacc attttcccgc ttccagctgg ggtagcttct gtcatataag cgcctctttt
    24721 tgctactaac gcaactgctt cagtgaagga tagcgcatta ctagctacta atgccgaata
    24781 ttctcctaaa ctcagtccta aagctgcatc tgggatgatt ccatgctcct tcaataagcg
    24841 ataatacgcg atgctgactg ttagaatagc tggctgtgta tattgcgtct gatccaaacg
    24901 ttcattttct gtaaagcaaa gttcagccat atcgtagccg agaatctcac ttgcttcatc
    24961 aaatgtctgc tttacaacgg cttcttcata taaatctttt cccatacctt gatattgtgc
    25021 accttggcca ctaaataaga atgctgtttt catgtttttc tccaatatat ccagaatata
    25081 tgtttgattc ttcctattca aaaaggccga gacattccta tttcaggaaa tgtcccagcc
    25141 cccaacctaa ttttaatgat tatgcagttt gtttttctac gtaagtcact aaatcttgaa
    25201 ccgttttgat tccgtcttct gtttcgattt tcacgtcaaa ttcatcttca atttcattga
    25261 tgatttggaa taaatccaag ctgtcagctt ctaagtcttc ttggatgtca gtagtcaatt
    25321 tgatttcttc agtttctttt cctaattctt ctgcgatgat ttcttgaatt ttttcgaata
    25381 ccataataat atattcctcc gatattgttt gctttttatt tttataattt aacagtctac
    25441 aactccatga gaatacttcc ccatgttaaa cctccgccat agcctgtaaa tattactttc
    25501 tgctgttttc ctaaggtgat ctttccacta gaaactgctt catccaataa gatagggatg
    25561 cttgcagcag atgtattccc atatttatcc atattggtca ggaacttttc ttgcggcatc
    25621 tttacttttc gggcaatttt ctcaataatt ctgaggtttg cctgatgcaa aagcagataa
    25681 tcaactggtt tgtctttcat gacttcttgg atattgtttg caacgtctcg tacggcaaaa
    25741 tcaaaaatat ctctgccagt catttgcaaa taatacgaac cttctgaatc ttctgaatag
    25801 aacggatttt tattttgctg gtagccagcg gtcaatgagc tacttctggt accgtcagct
    25861 gctaattttt ctttcagcaa ttttttctgt ggattcgctt ctaagagaat cccacctgct
    25921 ccatcgccaa acaacacagc agtcgtacga tcagaccaat cgattagctt agatagtgtt
    25981 tcaccaccaa tcaccagtcc cttagttgag ccgcagcgga tcattttttc agccatgctt
    26041 aacgcataaa caaatcctga gcacgcagca ctgatatcaa aagcgatagc atgagttgct
    26101 cccaatttcc cttgtacttg tgctgctaca gatggtgtcg tgtaatcggg cgacattgtt
    26161 gctactaaaa taaaatccaa atctttaggg tttagatttt tctgttttat caatttttcc
    26221 gccactaaaa tgcataaatc agaagtctcc tgatttgtta cacatcttcg ttcacgaata
    26281 cccgtccttg acgaaatcca ttcatcagag gtatccatca tcttggaaag atcattatta
    26341 gtaataatct tttcaggaag gacgctggct gttgcagtaa tcattccaaa ttgttccatt
    26401 aaatatcctc acttatagtc ttgcaagaag tcgtgcaagt ttttcagtgc atccagcaag
    26461 gcatgctgtt cctccgtagc cattccgtct aagatgttct tgaccatttc acgatgaaaa
    26521 tgctgatgga cccgaaatag taactttcct tttttcgtta gtcccaactt tacaacgcgg
    26581 cggtcatctt cactgcggat acgttcgaca tagccttttt ttactaactt gttaatagca
    26641 gtggtcaagg tcccaacagt aattgatagt tctttggcta cttctgaagt tgtttttttc
    26701 ttgtacatcc cgattgcctc gatcgtgtgc atctctgtga ttgacagatc tttgaattga
    26761 gattttttca gctcagactc ttcgattgtc aaaatatcat tgaagacact aacaagataa
    26821 tcatttactg tttttaaatc tggttccatg ttccaccgtc cactaattag tttgattatc
    26881 aaatcttttg atgttcaaac tatatcttaa cttttttcaa aatgcaacct taaaagatca
    26941 tttttttact atatgagctt tttttaagat tttactcatt taaaacaact gataaatcag
    27001 catttataat ttcttttatt agaaaaatca tctatctcac atattttgat tatcaaagta
    27061 ttttctgtga acagaactat agaattccct aaaacttgtc cattatttca aaatttgtta
    27121 tagtcttttt attcaaagcg taaaaggaga tcctatgtta aaagaaaaaa tgaaaactct
    27181 ccccatgctg ccgggtgtct atcttatgaa agaccgagat ggtaaggtca tctatgttgg
    27241 taaagcaaaa cggctcaaaa atagagtaag cagctatttt catcagaata aacagcattc
    27301 aaaaaaagta ttacgtatga ttcatcatat aacagatttt gatttcgtcg tagtagacac
    27361 ggaattagat gctttattgc tcgagtgtca attgatccaa cattaccgtc ctttttataa
    27421 ccgacaaatg aattatttca gtaattataa ttatgtacat attaccaata agggatttgt
    27481 attaacagat actcctacgg ctcgaacata cgggcctttt cgtctctata aaaaaatgcc
    27541 gtctattttg cgtataatgg aagaaactta tcaaatgcct tggttgtctg aaattagttt
    27601 gttagctttg cgagtccagc ttcctgacct acaggaaatg acttttgaac agaaaaaaaa
    27661 ggaactccaa gggctgtttc aaggccgaaa taaaaagcta ctgacttact tgaaaaaaag
    27721 acaacaacat ttcatctatc agttgaattt cgaaaaagcc ggtatgcttc aaaaagatat
    27781 cgaattagtt acttatttta ttagacggat ccaggaacaa aagcagtttt taagaacacc
    27841 atcgctcacc ttcagcatgc cattagcagc agatgagagt cagaaaaagc actacctcgt
    27901 ttgttatggt caacttgcag aaacaatgat tacctctggt gacgtttctc ctgacttcta
    27961 ttatgaaaag aaagaagctc atttatcttt gaaacgtcaa ttgtcaaaag aagaaatcga
    28021 tcctgtacaa atcttgatca gttaccgcaa aaaattagaa aaagaacagt ttgaaattga
    28081 actgttaaac aaaaaagaag ctgaaaagca actaaattag ttgcttctcg gcttctttta
    28141 ggcatcttca acttcaaatg tcaattatcc taaaatttat tggattaagt catagatttc
    28201 catcgctacg atatcgatat tatcaaattc gtattgctga tgagtatctg tttcagttaa
    28261 ttcaaatgta tcggtcgctt tatcataagt tactgtacac ttctctacgc cttctttttc
    28321 aaaacggcga acttgcagtt cattatctgc tgactcgacc atagcctcca gtcgttttat
    28381 gattgcgact aattgtgaat gtgtcatcgg aggtcctcct tttcttattt ctgttatatt
    28441 gtatcaaaag catagcctac ttgcattgat tttctctaaa aaaactcgtt ttttatgatt
    28501 ttttttctgg tatttttcct tcaggccacc aaagtttgat caaagcttgg gttccgtcat
    28561 tctcaaaacc tttatctgcc aactcttcat atagtttcaa tgcttgggta gttgctggca
    28621 atacaagatc cattttttct gcttcttcta atgcaatctt taaatctttg ataaaatgtt
    28681 tgacaaaaaa accaggtgtg taatcttctt ttaaaatacg aggagaataa ttgcttaaag
    28741 accagttagc tgcacttcca cctgaaagcg tctcaatcac tttctttaaa tcaagtccgt
    28801 ttttttgtgc atagacaagc atttccgtca ttcctgtcat cgtacctgca atcatgatct
    28861 gattagccat ttttgtatgc tgacctttcc cagcagaacc atgcaatgta aatgtcgtac
    28921 caaattcttt gaataaaggc agtacttgat cataaacggc ttcttctcca ccgaccatga
    28981 tcgtcagtgt accattcttt gctcctaagt caccaccgga taccggtgca tctaacgcat
    29041 ctgcgccttt ttcttttgct gtttttgcaa ttttttctgc caggcttggt gtacttgtag
    29101 tcaaatcaac taaaatatgt cctgagatat ctgcagaaaa aatccccgac tgtccatagt
    29161 agatttcttc tacatctttg gggtaaccta ccattgtaaa gagaatatta ctttgttctg
    29221 ctaccgcttt tggtgtatct gcccagatag ctccttctgt tactaaagga tcagcttttg
    29281 cttttgtccg attatagaca gctacttcgt gtcctgcttc tagtagatgg cgtgcgacag
    29341 cacttcccat gacgcctgtt ccgataaaac caagtttcat tttttcgacc tcctaaaaat
    29401 caaaaatcct tgcttctatg ctagtatacg ctagaaacaa ggattttttc ataaaaaatg
    29461 tttgaatttt atcttttaaa tgaatttaat tttattttga ttcgttcaat cgagtcttct
    29521 tcttcttttc ttcgcatctc ttcttcatga taaacaacca tgtcttgata aaattcgatc
    29581 atagcttcgc cgaaatgtgt agatgaaata tcataaagtt tagcttctaa aacttttgga
    29641 tctttaggaa cttcactgga aatataatca ataagtgctg gtgcaaagtc ttcgtcggtt
    29701 tcaaaagttt tccctaatga aggatgatcg aacaatcggt tcaaataagc atttccttcc
    29761 acgacacaag gtgttccggc tgccatcgct tctgtataag taagcccttg tgtttctgaa
    29821 gtagaagcac tgacaaaata atctgccgct ttataataaa gtgctacatc attgttgtcc
    29881 acttctcctg tgaactgcac aaattcttct aactgcagtt ctgagatcaa ttcctctaac
    29941 tcttctacat aaggtccttt tcctacgata actaatctgg cgttaggata agcggagatg
    30001 acctgaggaa acccattaat caaggcttgg atatttttct cataagaaat cctgcttaaa
    30061 gaaagaatca tcaaatggtt ttcctgaaga cctagtgact cccttaattt gctgatatcc
    30121 tgctgagtga tatcagaacg tttgaatttt tcaatgtcga taccagtagg aatcacgcga
    30181 agcggtgcta ttacaccata gctgcgtaat gtatcaataa cacgttcact tggacaaact
    30241 acacctgtcg tatgatttgc aaataatcta gacaagtatt ttacatggga ttgcctgatc
    30301 acttttcctt taccgatata atgaaggtag tcttcataca tcgtatggta agtatggatc
    30361 agaggtattt tcaacttctt agctaccatc ttcccaagta tccctgctcc gaattctgta
    30421 tgggtatgaa taagatctag ttctagttct ttggcaacca aataagcata ccacatgcct
    30481 ctaacaacaa ctcttcgatc cttgaaagaa ataaatggta cacttggcat tcgaatcaca
    30541 tctttttcca acttatccgc attaggatcg gtcgttgtaa aaatatagac ttcatgccca
    30601 tgtctttcta attcttgttt taatgttttg atcgatgtag caactccgct gacttgcgga
    30661 aagtaggtat cggtaaaaaa gccgattttc atgtttttcg cctccaaact agtccttgac
    30721 ctgatttact ttttttaatt tgctagagac tttttcattc agtacacttt cgtatacatt
    30781 tttaagttct tcaccgattt tattgatact tcgctctttt gctgtttgat aaccggcttg
    30841 tgtcgtactc ggtagttctt gattggtaat ctgttggatc agtcggataa attcttcgtt
    30901 tgtcttcccc atatagcagt tcttgctatc ttctaaccag ccattataca ctggaatatc
    30961 tcgaacaagt acgttttgtt tgcttgctaa agcttctaaa acaacgatcc cttctgtctc
    31021 ttcataagaa gggaaaaaga atagatctgc acctgaatac gcaccttcta tgatctctcc
    31081 acgaatgtaa ccaggaaaca ggacattttc aggatgatct tcttttacaa ttttccggat
    31141 gttccgagga atcgagtaca tgggaacatg accaaaccaa ataaatgtgt attcgggcat
    31201 cttcttagca atttctacga aatcaatgat tcctttacgt tcgaaaaaca aaccgacaca
    31261 aatgattact ttttgatccg gagacagttt gaaatattct ctgaactttt gttctttcat
    31321 tggatcaggg tgatatttct ctaagtcgat cccattggaa atagcttgaa tcggcacacg
    31381 gataccatag ctttccaaca actgttttga ataaggtgtt ggcgtgatca gatagtccgc
    31441 tttttggtac aatcctacca gatatttttt tacgatagga gaaagctggt tcgatccgat
    31501 aaaggaattt cggaaatctt cctctgtaga atgcgcatga tagacaactt tttttccttc
    31561 tcttctggct ttattgacca tattatgact attgattccg tatgtattga tgtgcaggat
    31621 gtcataatct tctttttcat ctagtgtata agagataccg acttccgtca gagcacgttt
    31681 ttgatgatca agcgcacgcc cgattccaga ttttgcaagc atcttttcac tttcaaaata
    31741 taataatacc ttcaccttac gtcccccttc acttatatag tataacatag cctacaaact
    31801 gtcactcaaa cttaagccct atttttgtct attcttactg acaaaaacgt tttcttaaaa
    31861 aagaacaaaa aaactggcgt agctctttta caagccacac cagcttttga tacgttgttt
    31921 attttacata ttcatgaaca agttcaacga cttcttccat catatcgcat tctttcaatg
    31981 cacgatcagc aagttcagcc attttcgctg tgtccaagcg tttcattaaa ctacgcgttt
    32041 taagaataga tgtcgcactc attgagaact catctagacc catacctact agcaatggaa
    32101 cagctgtttg atctccagcc atttctccac acatacctgc ccattttcct tcagcgtgtg
    32161 ctgcatcaat tacatttttg atcaaacgta aaattgatgg gttatatggt tggtacaagt
    32221 aagaaacgcg ttcgttcata cggtctgctg ccattgtgta ttgaatcaaa tcgttcgtac
    32281 ctacagagaa gaaatctact tcttttgcaa acttgtctgc taagactgct gctgctggaa
    32341 tctcgatcat gatgccgact tggatcgtat cagatacttc cttgccttca cttatcaatt
    32401 tttgtttttc ttcttcaaag atcgcttttg ctgcgcggaa ttctttgagt gtagctacca
    32461 tagggaacat gatacgtaaa ttaccatgaa cagatgcacg aagcaatgca cgcatttgtg
    32521 tacggaacat ttcgtcgcct tgttcagata aactgatacg caatgcacga tatcctaaga
    32581 atggattcat ttcatgaggt aattgcagat atggtagttc tttatctcca ccgatgtcca
    32641 ttgtacgaac aactacaggt tttccttcca taccttctaa tacagctttg taagcttcat
    32701 attggtcatc ttctgttggg aagtctggag aatccatata caggaattca gtacggtaca
    32761 gaccaactgc ttctccacca ttattatgga caccaactag atcttttggt gttccgatgt
    32821 ttgcagctaa ttcaaaatgc ttgccgtcag ctgtgacagt ttctgcattt tttaattttt
    32881 cccattctgc tttttgtgct gcgtaatctg cacctgcttt ttcaaattca gctttttgtt
    32941 catctgtagg atcgatgatt acatcgcctt cgatcccgtt gacagccaaa atatcgcctt
    33001 ctttgacttt agcagtaatt tcttttgtcc caacgattgc tggaatttct aaagaacgtg
    33061 ccatgatagc agaatgtgaa gtacgtccac caatgtctgt tacaaacgct tttacaaaat
    33121 tgcgatctaa ttgtgctgta tcacttggag tcaagtcatg tgcaacaacg accacttctt
    33181 cgttgatcat tgatggattt ggtaaagtaa cgcctaataa gtgcgctaaa atacgtttag
    33241 caacgtcacg aatatctgca gcacgctctt gcatataagc attatcttcc attgcttcga
    33301 acataccgat atacatatcc gttacttctt ttagcgctga ttcagcattt acgctgttgt
    33361 ctttaatatt ttgcttgatt tgacctacca tttctggatc tgaaagaacc atcaaatggg
    33421 catcaaatac ttgcgcttct gcttcaccta agctttgcgc tgctttttca cgaatttgct
    33481 gcaattcttc agtagattta gctaatgcag catccaaacg agcttcttca gccgcagtat
    33541 cttctactgt tgccttactg aatgttaaat ccggttgaac tagcaggtaa gcttttgcaa
    33601 cggctacacc gtcactagcg gcaatccctt ttagcatttc aaccattatt cagataagcc
    33661 ttctttcttc attgtttcaa cgattgctgc cattgcatca gcttcgtcag caccttctgc
    33721 agtaatagta acatcagaac cttgacctac acctaaagac ataacgccca tgattgattt
    33781 caagtttact gatttacctt tatactctaa gtttacatca gagttaaatt tgcttgctgt
    33841 ttgtactaat agtgtagctg gacgagcgtg gatcccagtt tctgctacta cgtgaaattc
    33901 tttcttttcc atattaatcg gtctccttta agtgagtgtt gtttttatgt gagggttttc
    33961 ttttttagta attcagagaa tcctaacaaa tgccaccctt tcatctgtaa agattaacat
    34021 tttttttcgt tagatacaac agttttcatt caatttcaga taaaagtatt caaattattc
    34081 cggattcttt ttcatccttt tcttcacatt ttataacaaa cgtgtttatt tcctttttct
    34141 tgtcgctttc tcttctcctg acgtttatca ttctttcctt tgaccctctt tgttttcata
    34201 tgaaacgaat tacttgcttc cctttttatt tttagtataa taagagtcaa aggtcaggaa
    34261 tggtcaatag agatttcacc ttttctacct cataaatctc ttaaaaaact aggggttatt
    34321 cttttacctc atgaaaaacg agatgtaaaa tatggatgta cccatttttg tgaaaggacg
    34381 tgaatgattc atgctttgtc aaaactgcgg caagaatgag gcaacgattc acttgtatgc
    34441 cactgtcaat ggtcagcgga cacaactcga ttactgtcag agctgttatc aaaaaatcaa
    34501 aaatcaacaa aatggaggtc tgactaatat ggcccaaaat gatccatttg gatttggcag
    34561 tttggacgat cttttccgtt ccatgtctcg ccaaatgcaa cagcaaggaa attatgagca
    34621 aacaccacca acacaatttg gcggaaacaa caacttcaat ggtggtcagc cacctcaagg
    34681 caatacagaa ggtttattgg gagaatacgg aatcaatatc acagaacaag cacgacaagg
    34741 tgatatcgat cctgtgatcg gacgcgatga cgaaatcaaa cgagtcattg aaattttaaa
    34801 tcgccgaacc aagaacaatc ctgtcttgat tggagaacca ggtgttggta aaacagcagt
    34861 tgttgaaggt ttagcacaaa aaatcgtaga tggtgatgta ccgcaaaaat taatggacaa
    34921 agaagtcatt cgtttagatg tcgtatcttt agtccaaggt actggtatac gaggacaatt
    34981 tgaagaacgt atgcaaaaat tgattgaaga gatccgtcaa gcagaaaatg tgatcctatt
    35041 catagatgaa gttcacgaaa tcgtcggtgc tggttctgct ggtgatggaa atatggatgc
    35101 tggtaatatt ttaaaaccag ctttagctcg aggagaattg caaatggttg gtgctactac
    35161 cttaaatgag taccgcatca tcgaaaaaga cgctgcttta gaacgtcgta tgcaacctgt
    35221 acgggtagat gaaccaactg tcgaagaaac gatcagtatt ttgaaaggtt tgcaaaaacg
    35281 ttatgaagac tatcatcatg taaaatatac tgatgaggcc atcgaagcag ccgcaacttt
    35341 atccaatcgt tacgtccaag atcgcttctt gccggataaa gccatcgatt tgctagatga
    35401 aacaggttct aagaaaaact tgacgatcca aatcgttgat cctaaaacaa tcgagaaaaa
    35461 actgcaagaa gcagaagaac aaaaagtttt agcttctcga gaagaagatt tcgaaaaagc
    35521 agcttattat cgcgatcaga tcaataaact tcaaaaaatg aaagaacgtc agctcactga
    35581 agaggaaaca cctgtgatta ccgaaaaaga tatggaaaaa atcgttgaac aacggacagg
    35641 tattccagtg ggtgaattaa aagaaaaaga acagacacaa ctgaaaaact tagcagatga
    35701 cttgaaagct catgtcatcg gacaagataa cgctgtagat cgagtagcga aagcgattcg
    35761 ccgaaaccgt gttggattaa acaaacagaa tcgtccaatc ggctccttct tatttgttgg
    35821 accaactggt gtaggtaaaa cagagcttgc gaaacaactt gcctacgaac tattcggttc
    35881 acaagattca atgattcgtt tcgatatgtc tgaatatatg gaaaaacata gtgtctctaa
    35941 attgattggt tctcctccag gttatgtcgg atacgaagaa gctggtcaat taacagaaaa
    36001 agtacggaga aatccttaca gtcttgtctt gttagatgaa gtagaaaaag ctcatccaga
    36061 tgttttgcat atgttcttgc aaatcttaga tgatggtcga ttgacagatg cacaaggaag
    36121 aacggtcagc tttaaagata cgatcatcat tatgacaagt aatgccggaa ctggtaaagt
    36181 agaagcaaac gttggttttg gtgcggcacg cgaaggtgtt actcgttctg tattgaacca
    36241 attaaacaac tactttacac cagaatttct gaaccgtttt gacgggatca tcgaattcag
    36301 tgcattatca aaagaaaact tgatgacaat cgtcacacta atgctagacg atgtcaacca
    36361 aatgctggca gcacagcaac ttcacatcga agttcctacg aatgtcaaag aaaaattagt
    36421 tgacttaggc tatgatccat ctatgggtgc tcgtcctctt cgacgcacga tccaagaaca
    36481 aatcgaagat ggtatcgctg aattttatct tgatcaccct tctatccatg agttgaaagc
    36541 aaaactggat aaagatggaa aaatcatcgt tacttctaaa ccagaacgtt tggctaaaga
    36601 atcagccgaa gaaacggctg aataaatcca gttttatagc gtgagacggt atttctttga
    36661 gaaatgccgt ctcattcttt tttcagaaaa gtcaaaatac tgttcatttt ataaaaaaga
    36721 aattataata agagaaacaa gcaattaaaa atacataaaa gaggtgagcg ccatgaaatt
    36781 ggtaaatgta acaaacagcc attcccgtct tgtattaaac cagcttgaaa acactgatgc
    36841 ccatctagtc aaggtctaca cagccggaaa taccactatt gtctacactg aagcccctga
    36901 acataatgaa attctgctga tcaatgacaa acgtaaaatc cagccgaaag aaatcgaaga
    36961 tgctaaaaat tatttcttag ataaaatcaa agacgccgtt taccacgagg atgaaatcaa
    37021 agtaatcgaa ttggcaggcc ttgttgaaat ctcgattcca aaacatgaat taggcgctac
    37081 attagccgtg taaacaaatt ttgaacataa taattaaaga gcgtgggaca aaa
//
LOCUS       DACKAC010000022        34748 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.022, whole genome shotgun sequence.
ACCESSION   DACKAC010000022 DACKAC010000000
VERSION     DACKAC010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34748
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.022"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(32..796)
                     /locus_tag="I0B46_001155"
     CDS             complement(32..796)
                     /locus_tag="I0B46_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAR2083469.1"
                     /translation="MIDLHCHILPGIDDGAENIDASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIDRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEQLFFELLQLGKTPVIVHPERNAHFRKDPNHMLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKMMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(824..1522)
                     /locus_tag="I0B46_001156"
     CDS             complement(824..1522)
                     /locus_tag="I0B46_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAR2083470.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENMSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(1535..2314)
                     /locus_tag="I0B46_001157"
     CDS             complement(1535..2314)
                     /locus_tag="I0B46_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAR2083471.1"
                     /translation="MEETISLKEIFETLRKHVITILISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQATSTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAPTQPKATNDKTAPSRRSRSRV"
     gene            complement(2330..3274)
                     /locus_tag="I0B46_001158"
     CDS             complement(2330..3274)
                     /locus_tag="I0B46_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAR2083472.1"
                     /translation="MAKRRRRSRGKKIFLIILAILVVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGHDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYRDAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(3315..4094)
                     /locus_tag="I0B46_001159"
     CDS             complement(3315..4094)
                     /locus_tag="I0B46_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083473.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHFNNQRQHHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNDLFTKNVSNWQQAVDDVVIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSLIEQIRNEKIRAAYASKAETVAQQMGYSNTSY"
     gene            complement(4479..6554)
                     /locus_tag="I0B46_001160"
     CDS             complement(4479..6554)
                     /locus_tag="I0B46_001160"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAR2083474.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKESKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(6556..7473)
                     /gene="glyQ"
                     /locus_tag="I0B46_001161"
     CDS             complement(6556..7473)
                     /gene="glyQ"
                     /locus_tag="I0B46_001161"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAR2083475.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            complement(7859..8671)
                     /gene="recO"
                     /locus_tag="I0B46_001162"
     CDS             complement(7859..8671)
                     /gene="recO"
                     /locus_tag="I0B46_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAR2083476.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQLMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTSEKDMSEKDLPKKEE"
     gene            complement(8814..9713)
                     /locus_tag="I0B46_001163"
     CDS             complement(8814..9713)
                     /locus_tag="I0B46_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAR2083477.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(9728..10129)
                     /locus_tag="I0B46_001164"
     CDS             complement(9728..10129)
                     /locus_tag="I0B46_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAR2083478.1"
                     /translation="MPMDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFSVIVGLILFVPKIYHLLFN"
     gene            complement(10107..10583)
                     /gene="ybeY"
                     /locus_tag="I0B46_001165"
     CDS             complement(10107..10583)
                     /gene="ybeY"
                     /locus_tag="I0B46_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAR2083479.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(10600..12741)
                     /locus_tag="I0B46_001166"
     CDS             complement(10600..12741)
                     /locus_tag="I0B46_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010772666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAR2083480.1"
                     /translation="MPFLLFLFFIIVCGVTFSSVKQKANDFREGQVAEESIRANKTIE
                     NTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELIDKTNDTINKSYNDKVDK
                     AKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNFYSTIFEMTETEIHTVRD
                     ESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQMIRYLVDQGIVVNDVLN
                     EKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKLELLGLTNQTTSIFPLIA
                     MVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVVLMKFFQLFQTEQAAYIP
                     LFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIGTNFLTVILMSYVFSGLM
                     ATIVRRKRISEQGLSAAMWVIIFPVMMDVILIIYQGMSFGDATTWLTLVCGFTGALFS
                     FLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEAPGTYHHSMMVANLSANA
                     VAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPHNFLLPEDSKHIIFGHVT
                     DGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPEVTEEQFRYPGPKPQSRE
                     AGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQLDDSGLTLKEIRKVEKS
                     LISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(12815..13786)
                     /locus_tag="I0B46_001167"
     CDS             complement(12815..13786)
                     /locus_tag="I0B46_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAR2083481.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            14492..15871
                     /gene="brnQ"
                     /locus_tag="I0B46_001168"
     CDS             14492..15871
                     /gene="brnQ"
                     /locus_tag="I0B46_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAR2083482.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            complement(16027..16473)
                     /locus_tag="I0B46_001169"
     CDS             complement(16027..16473)
                     /locus_tag="I0B46_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAR2083483.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(16500..16676)
                     /locus_tag="I0B46_001170"
     CDS             complement(16500..16676)
                     /locus_tag="I0B46_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAR2083484.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(16838..17266)
                     /locus_tag="I0B46_001171"
     CDS             complement(16838..17266)
                     /locus_tag="I0B46_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAR2083485.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            17358..18218
                     /locus_tag="I0B46_001172"
     CDS             17358..18218
                     /locus_tag="I0B46_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAR2083486.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLMQALQDALKEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            complement(18312..18605)
                     /locus_tag="I0B46_001173"
     CDS             complement(18312..18605)
                     /locus_tag="I0B46_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAR2083487.1"
                     /translation="MPDTQKTKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(18678..20405)
                     /locus_tag="I0B46_001174"
     CDS             complement(18678..20405)
                     /locus_tag="I0B46_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAR2083488.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLIDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDDEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAMRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIKKMTEE"
     gene            complement(20619..21545)
                     /gene="trxB"
                     /locus_tag="I0B46_001175"
     CDS             complement(20619..21545)
                     /gene="trxB"
                     /locus_tag="I0B46_001175"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAR2083489.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(21690..23123)
                     /gene="ascB"
                     /locus_tag="I0B46_001176"
     CDS             complement(21690..23123)
                     /gene="ascB"
                     /locus_tag="I0B46_001176"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAR2083490.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDKDHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     NEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDTVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(23123..24526)
                     /locus_tag="I0B46_001177"
     CDS             complement(23123..24526)
                     /locus_tag="I0B46_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAR2083491.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMATMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     DLIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            24756..25658
                     /locus_tag="I0B46_001178"
     CDS             24756..25658
                     /locus_tag="I0B46_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAR2083492.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            complement(25699..27585)
                     /locus_tag="I0B46_001179"
     CDS             complement(25699..27585)
                     /locus_tag="I0B46_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAR2083493.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEAGETDDRQKRSGKEIVKTPIQGQIAPLKAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSETGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEKEGYSLETPVIVTNYMDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(27682..28533)
                     /locus_tag="I0B46_001180"
     CDS             complement(27682..28533)
                     /locus_tag="I0B46_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAR2083494.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQNNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            complement(28868..30652)
                     /locus_tag="I0B46_001181"
     CDS             complement(28868..30652)
                     /locus_tag="I0B46_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAR2083495.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGVYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFVAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            complement(30652..32403)
                     /locus_tag="I0B46_001182"
     CDS             complement(30652..32403)
                     /locus_tag="I0B46_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAR2083496.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            complement(32545..32769)
                     /locus_tag="I0B46_001183"
     CDS             complement(32545..32769)
                     /locus_tag="I0B46_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAR2083497.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            33020..34606
                     /locus_tag="I0B46_001184"
     CDS             33020..34606
                     /locus_tag="I0B46_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAR2083498.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFVVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADQIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETIEEIE"
BASE COUNT         9554 a         7478 c         6267 g        11449 t
ORIGIN      
        1 cctcatttcc tttttatcta cgaataaaga actaaaataa accaaacttc ttctttttta
       61 cctcttcata gactggataa ctgatttcat ccccgttgat cagatccttt gcgacttgtt
      121 tcatctgtac tactttttcc ttaccaaagt ccttagcaat ctgctcatag cactctttta
      181 agtaaaaggt acgcttagag accccatggg catcagaagc agccatctgc acaagattat
      241 gtttgaccat cattttggct gttttctgga tgtcttttcc aaatacaccg acataacttg
      301 gtgcggtgag ttgggccaag catcccatat ctaggaatgg cagcatatga ttcgggtctt
      361 tacgaaaatg cgcgttgcgt tctggatgga cgatcactgg tgtctttcct agttgtaaca
      421 actcaaaaaa gagctgttct gcatataacg gcacttccag tgtcggaaat tcgattagaa
      481 gatatgtatc atctaaatct gtaaataaaa tttcatctcg gtcgatgtct tcgatcagtg
      541 ttccggtaat acgtacttct tgtccttcca ataacgtcaa aggcaattgt cttttctcta
      601 attctgcttg taacgaagcg actagagaaa tgacttgtga tttttcattg ctgtattttc
      661 cattattatg atggggtgtg cataaaatat gtgtgatccc ttgttgaatc gctttttcag
      721 ccatggcgat actggcatct atgttttctg ctccgtcatc gattcctggc aggatatggc
      781 aatgtagatc gatcatttct cttgtctcct ctctttttat ttcttaattc ccgtagtaat
      841 agccagcatc tttgctgtgt tctgccccat tgtatacaac accaaggact cgcgcttgta
      901 ccatatccag caattctttt gctttagtca atgattcttt ccgtgacata ttttcacgta
      961 cgaccaataa ggtgccatcc gttttggaag acatgatctg tgcatctgtt acggcaacta
     1021 ctggcggcat gtcgaagatc actacatcaa atagttgtct tgcttctgct aaaatctgat
     1081 ccattcttgg cgaacttagt agttctgatg gatttggtgg tttaggtcca cttggcagaa
     1141 tcgacaaatt ctcgactggt gttcgttgga tctcatctgc tacacttcct gatgaactaa
     1201 gaacggtact taatccgctt gcgttgttca acttgaacgt tttatagatg acgggcttac
     1261 gtaagtctgc atcgactagc agcacccgtt gaccagattt ggcaaaaaca acggcaatgt
     1321 ttgctgcagt ggtcgatttc ccttctccag gtcctgatga agtcacaaca atcgttttaa
     1381 tttgctgacc agctgcggag gcaaactgga tattcgtccg aatcgtacga tattgttcag
     1441 cgattggtga agaggggttt gtgagtgtga ccaaactgac tgcatgagtt tgggctgctt
     1501 tttgtttctg tgttcgtgcc atttttcttt cctcctagac tcttgagcgg cttctgcgtg
     1561 atggcgctgt tttatcgttt gtagcttttg gttgtgtcgg agctggtcct tttttacgga
     1621 taccagcatt caattctttc gctgacattt ctggtacagt ccctaagatc gggaagccca
     1681 atgtatccgt cacatatttt tcatctttta ctgtccggtc gagtaattca aataagaaag
     1741 ctagacctac gcctaccatc attcctaaca ctaagccaat cactagattc aatttattat
     1801 ttggcgaaac aggtgtcgta ttggcaactg cacctgagat gatcgagatt ttatcgacat
     1861 tcatcacgtc tttggcgttc tcttggaata cttgtgacgt tgtattagcg atttgttgag
     1921 ccgtcactgc atttgtgctt gttgcttgga tcgagaacat ttgtgaattt tgtgactgat
     1981 tgacggagat ggcatctttg atttgtccag cagtcatttt tacattgtct tctgtttcta
     2041 aacgatcttt gacttcattt agtactagat cactgacgat catatcctta tacgtattga
     2101 tcatttgcag gttcgtattg acatcattga catttgcagt atctgattgc ggcagtgtca
     2161 cgatcaattg ggcttgtgag ctgtattttg gcgtcaagat gaaaaacgtc acgatgcctg
     2221 agatggccaa gccagcaaac attgagatga ggattgttat cacgtgtttg cgtaatgttt
     2281 caaaaatttc tttcaagctg attgtctctt ccattttttc tccactttcc tatttattct
     2341 ctagattcat ttgatctttt aattcttttt tcacacgatc aagttcttga tcgctgactt
     2401 tttggtatga gatattgtct tgcatgaagc cttctccttg cagctgatct tgcttgatcg
     2461 taccaaatgc atcccgataa tctaaagcaa tctttttcat ttcatcaaag ctcatgtctg
     2521 ttttcatatt cgactctaga gcattcaaaa cggattgata gttgcttaca ccgttcagac
     2581 tcaacacttt tttcgctaca ccttctacga tctttctttg acgttcttgt ctgccgtagt
     2641 caccatttgg atcttcataa cgcatacgag aataagacaa tgcctgatcc ccgttcaatg
     2701 tgattttacc gatcgtaaaa tcatagccat cttgtgagaa ggtgaggtca ttattgactt
     2761 cgataccacc aactgcatca acgagttctt ttagaccttt catattgatt gaaacatagt
     2821 gatcgattgg gatgtctaca tatttttgta cggtatccat cgccatggca gctccgccga
     2881 atgcgtaagc gtggttgatc ttgtcttttg tgtcatgtcc aacgatgtcg acatacgtat
     2941 cacgaggaat gctgacaatc gttgtctgtt tgtcttcagg actgactgtg gcaaccatca
     3001 tcgtatctga tcttccttga tcgacccgac ctaaatctcc cgtatcaatc cccatcaata
     3061 gaacagagaa agattcttgt ttgtccagat caactggttt tgttcgttct ccttgggttc
     3121 gttccactga ttcgtaagtt ttttcaatag aatgtgagag atctagatat aattttgctc
     3181 ctactcctac gattgctaat actaatacga caagaattgc taaaataatt agaaatatct
     3241 ttttccctct tgatcttctt cttcgttttg ccatagtttc ctccgtttgc tagccttgct
     3301 tgaattctcg cttattaata gctcgtattg ctatagccca tttgctgagc gactgtttct
     3361 gcttttgacg cgtatgcggc tcttattttt tcattccgga tctgttcgat gagactgagt
     3421 gcggtcaaat attgatcgta agtagcagta gctgttactt gaccgtcttt cagcatcgta
     3481 tcgaatagct gatctagttg cgtgacttgc gcgatctgag tatcagcgaa tcctagatat
     3541 tgcataacaa ccgttttcca aggactatct ttaaaaaagc tcatattttc acgtacacga
     3601 gcgacatctg cacttgtcac cttttctttg atgacgacat cgtctacagc ttgctgccag
     3661 tttgatacat ttttagtaaa taaatcgttg acagctgtct ggatcttcag ttttgattct
     3721 gcatcctcta actgcgttaa gactgctttc ttttcttctt ggatcgcagc agcttcttta
     3781 ggtaattctg attccttgat gtcataatca tctgccgaaa gtttgataga agatagttta
     3841 tgtgacaaat ttgaaactgt ttcttctgtt atttcaggat ttaggaaaat tttttctttt
     3901 gtactgtaca gcttatctac ttcttttgat aaagaggtca cggtgtcttt ttgattggtg
     3961 atggcagatt ctgcgtaatt gatccgttga tgatgttgcc gctgattatt gaaatggtat
     4021 ccaaacaagc aaatcacaat agcgcctgtg cctaccgcca cagtagccaa taacttgacg
     4081 aacggctttt ccatagattc actcctaaat attgatgata gataaataaa gatgtgattc
     4141 attcccatca gaatgagcgc tttttatttt aaaagcataa ttaaactaat atattcatag
     4201 aaatatttat aaacaggttt tccctatata atcataccac aaaaagtttt cttgactagg
     4261 gaaattccta atttaaaagt aaataaaaca gtaacaagat catgattaat cgttttcatt
     4321 tttatttcct ctcttttcat gagcattttg aaaattcgaa caaagaataa aataacaaaa
     4381 attattaaaa tagaattaat aacagctatt tatatttata acaaaaaaac agagacacag
     4441 cacgtttcgt gcatgctctc tgtttttcaa acaaaagact attttgtgat gatttgatcc
     4501 aagcttgcta atgacaaagc catggcagct atttgactaa gttgtgttag tcggttctta
     4561 cgtactgctg gttcatcagc catgaccatc gtttgatcaa aataattttc gatcagtgga
     4621 cgaagagcga caagctggtc gtagttttcg tggatcgttt ggtcagcaaa acttgctttc
     4681 attgcttcta ctgctttgtg taattctttc tctgcgtcgt tttcgaacag ctcaggatct
     4741 acttctgatt gtttttcaag ctgcgctttt ttcgctaagt tcacgacacg tgtcaaagct
     4801 tccattgaag gtttgaagtc ttcatctgca agatgttgtt tgaacaattg tgctgctgca
     4861 aatacttttg tcagatcttt ttgttccgca ttcaaaacag catcgatgac atcatgacga
     4921 atattcttcg ttgtcaataa ttggcgcaga cggcctttga taaagtcgat cacttgttgt
     4981 tgcccttctg ataaaccaat gccgaaacga tccgtatctt tactgataac atcgctaatc
     5041 gtttcttgca gaacagacaa aggaaaggcc cattttttcg attcgatgat ccgaacgaca
     5101 ccgtatgctt ggcgacgtaa ggcataagga tcgtttgaac cgcttgggat catcccaaca
     5161 gcaaagaaag aaaagatgct atctaattta tctgcaatcg ctaaaacaga acctactgta
     5221 gactgaggta gctctccttc actggatata ggcatgtaat gctcacgaat cgctgtcgct
     5281 acagcaggtt tctcgccttt cagcagagca tatttttcgc ccatgatccc ttgcagttca
     5341 ggaaattccc ctaccatgtt ggttactaga tcaaatttat agatttcagc agctcgttgc
     5401 agatccatca attcttcctc agatagtcct acttgttcgc caatgatacg ggcaatgact
     5461 tgtacccgtt gcattttctc atagatacta ccgatttttt catggaaagt aacagttttt
     5521 agtttttcaa cgtattggtc gattgaagct ttcttgtctt cattataaaa gaattccgca
     5581 tcttctaaac gagccgtcaa tactttttga ttgcctcgaa ccacattttc caattttacc
     5641 cgatcaccat tacgagcaga aataaaatga ggaagcagca taccttgatc attccgtacc
     5701 tcgaaataac gttgatgttc tttcatagaa gtgactagta cttcttcagg aacagaaaga
     5761 tatttttctt cgaagtcgcc agaaaagaca gttggatatt cgactaaatt gttgacttct
     5821 tctagtaaat cttcgtctag atcaataacc caatgatttt tttcggctaa ttcattggct
     5881 tgtgtaacga tcatttcttg tcttttttgc ggagacgcaa tgacgaattg ttcattcaat
     5941 ttttcttcat attcttttgc atgcgcaaat gtcacatctt cgcctaagaa acgatgacct
     6001 cgagaggctc tgcctgtttt gacatccaag atttcgaatg ggattacttc gtcatctaac
     6061 aatgccacga tccagtgaat agggcggata tattcaaaat cgtattctgc ccaatgcatc
     6121 gttacaggga aagtcaggct tttaatgaca tctgctaaac ctgtcaatac ttctttggat
     6181 tccttaccat gagtgtattt cgttacatag acatattcca caccttttaa ttctctaaaa
     6241 gtgatagcgt ctgtcgtcag accttgtccg cggacaaatc cttccgctgc tttcgtccag
     6301 tttccatctg cgtccaaagc aatttttttg gctggtcctt tgacttcttc ttcactgtcc
     6361 gcttgtcgtt ctggaatgtt tgttactttg attgctaaac ggcgcggtgt ggaaaatgtt
     6421 tcgatcgtat cataagtcaa ttggttttca tctaaaaatt ttgctgtttt ttcttctaat
     6481 tgtttcatgc ttggtgtgac aacatgagca ggcatttctt ccagcccgat ttcaagtaat
     6541 aagtcttttg ccatcttatt cagcctcctt cacgttttct ggatttgctg tgtttttatt
     6601 caacaaaggg aatcctaatt tttcgcgttc tgccacaaag attttggcta cagcacgagc
     6661 catgttgcgg atgcgagaaa gataaccggc acgctctgtt acggatactg caccgcgggc
     6721 gtctaataag ttgaatgtat ggctacattt caagatataa tcatacgccg gatgaacaag
     6781 attttcatcg atacagcgtt tagcttcttt ttcaaaacga tcaaagttgc ttagtagcaa
     6841 gtcttggtcg cttacttcaa aagaatattt agaatgttca tattctggtt gtttgaagat
     6901 ctcgccatat ttcacgccat cagcccattc taaatcatag acactttcaa cttcttggat
     6961 ataagaagca agacgttcta atccataagt gatctcagaa gttactggtt tacaaggcag
     7021 tccaccaact tgttggaagt acgtgaattg tgtgatttcc attccgtcaa gccatacttc
     7081 ccaacccaca ccggcacagc ccatggatgg gttctcccag ttgtcttcaa caaaacggat
     7141 gtcgtgttct agtggatcga ttcctaaaag gcgcaagctt tctaaataaa gctcttggat
     7201 attctcaggt gaaggtttca tcacgacttg gaattgatga tgctgataca aacggtttgg
     7261 gttttcgcca taacgtccat cagctggtcg gcgagatggc tctacgtaag cagcattcca
     7321 tggttctggt ccgattgccc gtaaaaaggt atatgggctc atcgtacctg ctcctttttc
     7381 tgtgtcatac gcttgcatca gcatgcagcc gtttgatgac caaaattttt gtaatgttaa
     7441 aatcatatct tggacggtca attttttact catctgtttt gcccctttca attttaaagc
     7501 gttgctgtca aataagaata caggaaagaa aacgacaaaa aatccctatg tctgattaca
     7561 gacataggga cgaataattc gcggttccac cctacttccg aataaagatt cggcagcttc
     7621 atttatcgta ctcgcaagtg cctcttcaaa aatcgtttgg gtccggctcc cactctcccg
     7681 aactcgctaa accacgtgtt ttttactgat cttggtcaat gtacgtattc attttgcagt
     7741 tttcttgttt ctataataga tcctctcagt gaacgatcta tttgatctac ggttaataat
     7801 agacggaact ccctgtgttt gtcaaatggt tttgattttt tgttgtatgt ttcatttact
     7861 attcttcttt tttaggtaga tctttttcag acatgtcttt ctctgacgtc tttaatacgt
     7921 gtgcccaaga cttcatttga tcaatgaact ttttgctttt caaatggata ccgacatatt
     7981 catcatacag ctggtcgatg agttcacgga ttgcttgttt ggtttcttct ttcagatgga
     8041 ttccactgat tttatcataa gaaatagctg aaaacatccg aacgaaataa acagctcgag
     8101 gatctgcatg ataacggtga tcatccatcg accagtgtct ttcgcatatc acaccaccat
     8161 atttggaaga atagtcaaat tttccttttg tttccccaca tacgacgcag tgtgtccaga
     8221 ttggtgcaat tccgaagcgt tgcagtattt ggacttcaaa aatattcgtg atgatttctg
     8281 catcatctcc ttggtccatc agttgcagtg cttgttgcgt aaactggaac aaatgcgggt
     8341 cgtactgatg atcttcgatt gccgcatcta ctagattcaa taaatatgtg ccatagccgc
     8401 tcaaaaagat gtcttcttgg atgtgtcgaa acgcgtggac atctttgaca ctgcttaaaa
     8461 atgacaaacc ttcacttcga aaattcccga tataaactgc ttcagtaaaa gggagcaaag
     8521 ccggagctaa gggctgattt tgccgatgga tccctttgat ataaaacatc attttgccag
     8581 aagattccgt aaagattttg actaatttat ctttttcttt gtaattacgg ctagataaaa
     8641 tcaagccttt tgattctgca agctggctca tattctccct cctaaaaaat aaatctggaa
     8701 aagcagattc tgccgatcca gatcatttat gttgcgatct tccgcagctt ttctcaacac
     8761 gcggattttt atccagttta tttctatacc taacttacaa gaagctcaga atatcaatat
     8821 tcgtcttttc ggtagccgaa gtcttgcaga tagacttttt tgtctcgcca gtctttttga
     8881 actttgaccc aaagttctag aaagactttg tctcctaaaa gattttcgat atcttttctc
     8941 gctttcgttc ctacttgctt cagcattttt ccgcctttgc cgataatgat ccctttttgg
     9001 ctatctcttt cgacaataat cgtagcctgg atatggattt tatcattctc gtttcgcttc
     9061 atcgaatcaa caacgactgc gacagaatga ggcacttcgt ctcgtgttaa aaacaagact
     9121 ttctcacgga tcaattcaga aacgatgaag tattcaggat gatcagtcac ttgatcatct
     9181 ggaaaatatt gtgggccctc tggcatttca ctgactaata catccatcaa acgttcgacg
     9241 ttgtttcctt ctgtagcaga aattggaatc acttgtgcaa acggcatctg cgttgaataa
     9301 tcttcgatga ttcccaacaa ctcgtctgga tggattttat cgattttatt gataatcaga
     9361 taaacaggag aatgcacgtt ttttagacgt tcaataataa aatcgtctcc ccgaccgcgt
     9421 ttttgatcag cgctgatcat aaataagacc gcgtccactt ctttcaacgc actgtaagct
     9481 gcttctacca taaaatctcc taaacgatgc tttggtttat ggattcctgg tgtgtcaata
     9541 aaaatcaact gagcttctgg tgtagtatat actccttgga ttttatttct agttgtctgc
     9601 gccttatcac tcatgatggc aattttttga cccacgattc gattcaataa tgtggatttt
     9661 cctacattcg ggcggccgac gattgcgaca aaccctgatt tatgttctgg catatacgtt
     9721 ctccttttta attaaacaac aaatgatata ttttagggac aaataaaatc aacccaacga
     9781 tgacggaaaa acaagcggtc actaaaactg ctccagctgc catatctttg atttttttcc
     9841 caatcggatg aaaatgaaaa tccgtaaaca tgtccacgac attttcaaaa acagtattga
     9901 tgatctctac tatccatacg agaaaaacag ctaaaagcag ccataaccat tctatccgat
     9961 caagctggaa aatcacgccc ataaacaaag cgaggattcc gaacgcaaca tgtttcctca
    10021 tgttgcgttc ttcctcaaaa acggttttga ctccttggac ggcaaattcc acggacatca
    10081 ggaaatgttt gtttttctct acgtttttat cttttaagtc cataggcatc taagatctct
    10141 ttctgtaaac caaacatttc tttctcatcc tctggtgtca tatggtcata cccgttgata
    10201 tgcaagaatc catgaaccgc taaaaagcca agttcccgat catacgaatg gccgtaagct
    10261 tctgcttgct ctgcagcacg ttcagtagag atcatgatgt ctcccagtac acgaggcaat
    10321 ggattctctc catcttcaaa aatcactggg atttcgtctt cgccctcttc ttccaaggca
    10381 aaactgatga cgtctgtcgg tgcatctttt ccacggtaat cacgattgat tacttggatc
    10441 gctgcattgt ccatgaaagt aacagacatt tctgtttctt ctggcaactc taaatatgat
    10501 gctgcaaatt gcagcaagtc gtcgatttct ttcattttct cttctgagac ttggtttgtt
    10561 tcatccatga aagtaatatc cattatacat cgcttctttc tattcttctt gttcttcttt
    10621 tatcttctca acttccgatt tcattttagg atatttgatc cgagaatgga aggtactgct
    10681 taaaccgctg atcaatgatt tttctacttt acggatttct tttaacgtca atccactgtc
    10741 gtccaattgt ccatcggaaa tacgctcttc aataagatta tggacaaaac tttcgatttt
    10801 ttcgctcgtt ggatggtcca ttgctcgtac tgccgcttca caactgtcag ctatattgac
    10861 gattcctgct tcacggcttt gaggttttgg tccaggataa cggaattgct cttccgtgac
    10921 ttccgggttg cgttcctttg ccttaacgta gaaataacgc atcagcgtgg ttccatgatg
    10981 ctgtctgcaa atatcgatca ccatttgcgg catattataa tcttccaata ttttggcacc
    11041 atctgtcaca tgtccaaaga taatatgttt actgtcttct ggcaataaga aattgtgcgg
    11101 attttccgct cctgaaggca agttctcaac gaaaaagctg gcgtgtttga tcttgccgat
    11161 atcatgataa tagcaagcta ctcgagttag taaagaacgt ccaccgattt ctgctacagc
    11221 attagcactt agattggcta ccatcatgct atggtgatac gtgcctggtg cttcttccag
    11281 caactgtttc aataaaggat gattaggatt gctgagttca tttaatacga tcacactatc
    11341 atcattgacc atcaattcaa tataggggtg cagacctatc gtcagcaaga atgagaacag
    11401 tgcacctgtg aatccgcata ctagcgttaa ccaagtcgtt gcatccccaa agctcattcc
    11461 ttggtagata atcaaaatga catccatcat cacaggaaaa ataatgaccc acatagcagc
    11521 ggataaccct tgttctgaga tccgtttgcg tctgacgatc gttgccatga gaccagaaaa
    11581 tacataggac atcaaaataa ctgtcagaaa gttcgtgcca atcgaatcat aaaaaatgaa
    11641 aagtgcggat actgcttgaa aaataccagc catgatccct gctcttcgat tcaagaaaaa
    11701 gcttaaaaca agtggagcaa aagccgcggg gtaaaacagc ggaatataag cagcttgttc
    11761 tgtttggaag agttggaaaa atttcattag tacgacgctt actgacattg ccgtcacgta
    11821 gaaaagtacg tattttgttc tttctcctgc attttggaat tgaagtgaat tataaatcag
    11881 tacaccgatt tgaagcaaaa cagctaaaac catggcaatg agtgggaaaa tagaagtcgt
    11941 ttgattcgtc aatcctagta attccaattt tttcatcgct gaagcatcaa tttgatttcc
    12001 ttcgcgaaca ataatttctc cttggaagat catcactggt tgtacagctt ctcgtgcaga
    12061 ttgttttaat tcttctgtct ttttctcatt caatacatca ttaactacaa tgccttgatc
    12121 cactaagtag cgaatcattt gttgctgctc tgttgtgaca ttcaaatatt gaatctgatc
    12181 ttctgcttct tgtttaaaag cttccaattc gctttcacga acttgtttac tcatctgttc
    12241 gtcaatgagc ttcaaacttt catccctaac cgtgtgaatt tccgtttcag tcatttcaaa
    12301 tatcgtcgaa taaaaattgt tcggcagttt ttggtaaaac gcaacgtttt ctgcattcac
    12361 gttttcaaaa tttttcttca gtgtagcaat ccgctcttct gtagtaggtt tcgggatatt
    12421 ttcatcttcc ttcgccttat ctaccttgtc attataagat ttgttgatgg tatcattcgt
    12481 cttatcgatc aattcgaata attgcttgat ccgattgttc tggtcatctg ccagatcttt
    12541 ttgataagta tattctggag tcactgcttc tgctgccagt tttcttcttt gttcggtttc
    12601 ttccgtgttc tcaatcgttt tattcgcacg gatactctct tctgctactt ggccttctct
    12661 gaaatcattg gccttttgtt tgacactact aaatgtaacg ccgcagacaa tgataaaaaa
    12721 caagaacaga aggaaaggca atgctgcctt tccgagtttt tttgtagttt ctgcagtgtt
    12781 ttattcaaca tgtgcttatc ctcctaagca aagcttattc ttgatgaaga tccgcctttt
    12841 cataagcttt gatgatttcg gcaactacag ggtgccgaac aacgtcactt gcttcaaaat
    12901 taacaaaatc gatttttttg atttgcttta atgttcgttc tgcatgaaca agaccacttg
    12961 tcgtcccacg aggcaagtct atctgacttg tatccccatt gacaatcatt tttgattgat
    13021 aaccgagacg ggtcaagaac attttcatct gagcgactgt cgtgttttgt gcttcgtcca
    13081 agataacgaa tgcatcatcc aatgtccgcc cccgcatgta agctaaaggt gcgatttcga
    13141 tcacgcctcg ttccatcaat cggttcgtat gatccaatcc aaaaatttgg tataaagcat
    13201 cataaacagg ccgcagataa ggatccactt tttctttcag atccccgggt agaaagccta
    13261 ggttttcccc tgcctccaca gcgggccgcg tcaaaatgat tttttgcact tcgccttttt
    13321 ttagggcagc aattgctaat actacggcaa gaaacgtttt ccctgttccg gctggcccta
    13381 caccaaatac gatgtcatgt ttggctaccg attcaacgta tttcttttga ccgctgtttt
    13441 ttacccgtat aggttttcca ttacggtctt tgacgatctc ttcctcatac atctcaacaa
    13501 aataatctaa tgtacctttt tgccccatct tcaatgccgt cacaacatct ggcgtactta
    13561 cgtggattcc tcgtttgatc aattcctgta atgtccgaat gactgaggca gccaaggaaa
    13621 tctgggattg ctcaccaatg atctgaatga tttctccacg tgtatggatg gttgtttccg
    13681 ttgtttcctc gatcagtttt atatgcttgt cgtgtgcacc taaaagcatg ctgacatcat
    13741 cagcagctgt caatctaatc tctaaagaac tagactcttc cgtcaaaaat agacatcccc
    13801 tttttttcga ttcattcttt cctattttac catactcaga agaaactata tagcaaataa
    13861 gaaaaggttg taaaagagtc gtttaaccat acgtaccaag aaacgttaaa aaatagccgt
    13921 ttccattcgc taaaggtctc gttttgataa aaagaagctg agaatgtaag ttatatgaaa
    13981 gtggagaaca aaatattccg tttcattcag caggtattct ctaggtattt ttcttgtttc
    14041 aaaaaaagaa aacaaaatag ttaaaatatt cttgcatttc aaaaaaaaca ggtgtattat
    14101 tacgtcaaca aataaacata cagcaaagcg tcgaggaaaa cagtaggcta ttcaacgatt
    14161 tattcccagt gagtcatgta agtgagaaat gacaataaat cgaatagacg aacatcattt
    14221 ccgagtcaat gtcgaaaacg tagatcattg tggctgcctc cattatcagg ctagcggata
    14281 actttatcaa aaagcgtccg tgaatgaaga agctttttta agcgaaaaaa ggtggtaccg
    14341 ttctagaatc tagagccctt ttcccaagtg gaaaagggct ttttttatat tttaaggtgg
    14401 tggttttcat ggaatctgat attagtggca ttactaattg ctgcaacgtt tatatcgtca
    14461 atcaaaatga taattattgg aggaaaaatt tatggaagaa acaaaaactt attcatggaa
    14521 acaactattg atggtcagtt cacttatctt cgggatgttc ttcgggtcag gtaatctaat
    14581 ctttcctgtc cacttaggtc aaatggctgg cgcaaactgg gtcagtgctg gcgtaggatt
    14641 tgccatctcg ggcgctcttt tcccattact agctatctta gctgtagtag tgacaaaaag
    14701 tgatggtctg tatgacttag ctaaacctgt cggaaaatgg tatgctgcct tgttcttaat
    14761 ccttgttcat ttgacgatcg gaccattttt cggtacacca cggacagctg ctactgcttt
    14821 tgaaatggct gctaaaccat ttttgcctga aaaatataat tcattaggta tgtttttatt
    14881 ctctgctctt ttctttttac ttgcttattt ccttactgtc catcccacac ggttggtcaa
    14941 atacgtcgga aagtatctaa actccttgtt tcttgcattg ttagcaatca tttttgtcgt
    15001 ttctttattc cacccaatgg gaaatctaaa tcaatctgct gcagatactt accaaacaaa
    15061 cgctttaaca aatggtgtcc tagaaggtta taacacggtt gatgcagtag ccttgcttgc
    15121 cttaagtgtc acttttgtcc atgcagtccg tgaccttggt ttcaaagata aaaaagtcac
    15181 agaattaaca gcaaaagctg gtagcctgag cgttgcgcta gaagtcatga tctattttgg
    15241 tcttgttctt ctcggtgcga tgagtttgaa taagatgtct ttatcagaaa atggcggaac
    15301 tgcactttct cagatcacta atttttattt aggtaacttc ggcgctagct tcttaggagt
    15361 gatggtcaca ttaggtgtct tcactaccgc tatgggactc gttgtttctt ttgcgcaaga
    15421 tttccataaa ctgttcccta aagtcagtta tatgacatgg ctgcggttaa cgactttcgt
    15481 ctcttttgtc gttgcaaatg ctggtttaga caatatcatc caatggtctt taccagtatt
    15541 gatgcttttg tatccattgt cgcttgcgtt gatcttgctt tccttaacag caaaattttt
    15601 ccaaaagaca ccatttgtct atcaagtaac gatgttgttt gctgctgttc ctgccgtatt
    15661 ggatatgctg gcaaattcgc ctgctttagt gagtcaacaa agagttgtag caagcatgct
    15721 cgaattttat catcatcacg ttccttttgc tgcattagga cttggctgga tggtcccaac
    15781 gttacttggt tacgctggca gcctactgtt ttattacgcc tatcgcctat cagggtataa
    15841 acaagaagca aatgaactac cagaagaata accaaatcaa acacaattta acataagaaa
    15901 tattaaacag gaaaaaacca gctagatgaa agaatcctat tctttcgtct agctattttt
    15961 ttgttctctt ttttacgcgc acaaaaaaac tgcggacaat gattgtccgc agtttaaatg
    16021 ataaaattaa gattgtaata gttctttcac aatggcattt acttggtttc catctgcttt
    16081 tcctttgact ttaggcatga tcgcacccat gactttgcca aattctttca ttgatgaagc
    16141 accagtttgt gtgattgctt cttgaacaag ttgtcgaatt tcttcgtcgg aaagttgttc
    16201 cggcatgtat ttttcgacta tcacaatttc actttttact ttttcagcta aatcgtcacg
    16261 tcctgcttct tcaaactcat gcaaagaatc acgtcgctgt ttcatttcgc gagacaacac
    16321 cgttaattct tcttcttctg tcaagtcgtg accttcttta atctgctcgt tttgtataga
    16381 tgacttgatc attcggatga cttgtaaaga ttccttatct ttcgctttca tcgctgtttt
    16441 catgtcatcg tttaatctac ttagtagtga cacactacca actccaattc atcacagaac
    16501 tagaattttt tgcgttttct agctgcttca gattttttct tgcgtcttac acttggtttt
    16561 tcgtagaatt cacgtttacg agattcttgt aaagtacccg cttttgacac ggaacgtttg
    16621 aagcgacgaa gagcatcgtc aagtgattcg tttttgcgaa cgactgtttt tgacatgtta
    16681 attccctccc tccgagctta aaaagtaacc gtaattgtta ttgaaatata tctcaaaaaa
    16741 caagactgtc ctgtaaatat tatatctaat gctgttttga ccgtcaagga aatcttgctt
    16801 ttttttaaaa ttatcaaatg ttattttatt taaatcctta ttgacaattt tcacatcttc
    16861 cgaaaatctc gaatctatgc ccttcgatcg tacatccacc gagctgttct tcgaaataat
    16921 tcattggaca catatggatt tctttcgttg ttccgcacac cgtacagata aagtgatggt
    16981 gatggccgat ttccccttga cagcaatgaa aacgaaactt catctctcca tttaaatcgg
    17041 tttcttccag taaatcgatt tctgcaaaat catggagatt tcgataaatc gtatcgtaac
    17101 ttactccttt gaattcctga ttcatatagt caaaaacttc tttagcggat acgtagcgat
    17161 tgcgcttcac taaataagtt aacaatgctt ctcttttttt agtatattta aaaccgttat
    17221 ttttcaatgt ttctagtgat tgttcaatca atgtctgttt tttcatgact tattcctcca
    17281 aatcaaaatg attacgaagt acacattcta gagtatggta taataaacat aaaaaaaatg
    17341 caagtgaggt tttatccatg tcttccgatt ctacagaaat cgttactttt tttgtcatct
    17401 ctgactctgc tggagaaact gcgacaaaat tagcgcaagc cactatggcc caatatccta
    17461 ccgttgagtt caatttattc cgcagaacat ttgtgacaga tgagaaaaca ttgatgcagg
    17521 cactgcaaga tgcactaaaa gaaaaagccc ttgtcctgca cacattgatc aatcaagaat
    17581 tgatcgagat gacgcgttct ttttgtgaag aacatcaatt atttagtttt gatgtgatga
    17641 ctccgccgat atcagaaatc gagcgcttaa ctggtatcaa gcccattcgg caacctggcg
    17701 cccttcattt attgaatgag aactatttca aacggatcaa agcaatggaa tttgctgtaa
    17761 aatacgatga cgggaaagat cctcgtgggt ttctagaagc agatgtcgtt ttgctaggcg
    17821 tatccagaac ttccaaaacg cctttgagct tgtttttagc taacaaaaat ttaaaggttg
    17881 ccaatctacc tttgatccca caagcacata ttcccaaaca gctttgggag atcgatccta
    17941 aaaaaatcgt gggactgacg aataacccag atattttaaa caatatccgt aaagaacgga
    18001 tgcgctctta cggattaaat cctgatactg cttattccga tatcgaaaaa atacgagctg
    18061 aactcgattt tgcgaatgat ctttatgaaa aattgggctg cgtagtaatc aatgtcgctt
    18121 ctttatctat cgaagaaaca gcttcactta ttttgaatgc tttagaccta gaagatcata
    18181 gttattatgg aactgaaacc accgaagaaa cagactaaca gtttttcttg tctttttctt
    18241 aaaaaaactg gactgtgtca gcgaagaaga ttcgttgata cagtccagtt tgtctctatt
    18301 tgcacctatc gttattgatg ttccacatgt ttgagcgtct ggttcaaaat atccagtacg
    18361 tgagaatcat caagactata caaaatcgct tttccctcac gatgggactt gacgacatgg
    18421 ttttctctta ataaacgcag ttgatgcgat acagcagatt gttccatttc tactgcttgg
    18481 gtgattgctg tcacattttg ttccccgtct ttcagataca gcagaatttt gagacgagtg
    18541 gggtcactca acattttgaa tagttggctg actttctcta cttttgtttt ttgcgtgtct
    18601 ggcaatccat tcacctccaa cttgaaaaaa gactgacttc taaagtttac cactttttcc
    18661 atcagtctca agttttttta ttcttctgtc atttttttga taatcgcttc ataagcggct
    18721 actttttcag ctgcgtcttc tgaagagttt ccttttacac cgatataaaa tttgattttc
    18781 ggctcagttc ctgaaggacg gatagcgatc cagccatttt cttccagcgt atacttcaat
    18841 acgtcagatg gcggtgtcgt tagtttttct acgtttcctt cacggtctgt tctcgtcaac
    18901 tctttgaagt cttctgtttg gatgactttt gctccggcaa attctgtcgg tgcttcttca
    18961 cggaattttt ccatcaaagc agagattttt gcagaacctt cttgtccgct cattgtcaca
    19021 gaaatcgttt tttctgcgta gtagccatat gtttcaaaaa tcgtttgcaa gccgtcatac
    19081 aacgttttgc cttgtttttt atagtaagct gctacttctg ctaataagag caatgcttga
    19141 atcgcgtctt tgtcgcgaac gaaaggttta actaaataac cgtaactttc ttcgaatccg
    19201 aacataaaag tatgggaatg atcttcttca aattgttgaa ttttttcagc gatgaatttg
    19261 aatcctgtca atacattgaa catggtagcc ccatagtgtt ccgcaattgc tgtaggcaat
    19321 tcgctagaaa cgattgattt caataccgct gcattttcag gtaaagtgcc tcgcgattgg
    19381 tgtgcttcca aaatatattg agcaaacagg gctccgattt ggttccctgt caatacttca
    19441 taggagccat ttggcaaacg aacggctgcg cctaaacggt ctgcatcagg atctgttgca
    19501 atcagtaagt cagcgccttc tttctcgcca agtcgcatcg catactcgaa agcagagtgt
    19561 tcttctggat tcggtgattt gactgtagtg aaatccgggt ctgcgacagc ttgttctgga
    19621 actagtacga attgttcgaa tccagcttgt ttcaacgcac gttcgcccaa catttttcct
    19681 gttccgtgaa gtggtgtgta cacaagtttc aaatctttac ccattgtttc caccaattca
    19741 tgatcaatcg ttactgcttt gatttcttct agataagcgg catccacttc ttctccgatg
    19801 atcgtgatca atccgctatg ttcagcttcg tcgtcagata atacttcaac ttttaacgga
    19861 ttgctgactt cacggacata tttcgtcaat gcatctgcgt ctgcaggagg catttgtccc
    19921 ccatcttctc cgtagacttt atatccgttg taagctgctg gattgtggga tgcagttacc
    19981 atgattcccg caaacgtctt caaatgacgg actgcaaaag aaagctctgg tgttggacgt
    20041 aaactttcga atacataaga agggatattg tgatgtgcta atgttttggc tgcttccatg
    20101 gcaaattctg gtgattgatg gcgtgagtca taagcgatag cgacacctcg gcgttttgtt
    20161 tcttcacctt gtgtatccat gaaacgtgca agtccttctg ttgcttgtct taccgtatag
    20221 atgttcattc gattgatccc aggtcctaag attccccgca tgcctgcagt accgaattcc
    20281 aacggtgcat aaaaagcatc ttcaagtttt tcaggatctt cacccagatc aattaattgt
    20341 ttttttaagt tttcttctaa attttcttca tgtacccatt gttcatacac ttgttcccaa
    20401 gacatgaaaa taacacctct ttctgaaata tggttcttaa taagtatagc attcacttat
    20461 tagatatgaa agtacgaggt ctaaaaaacg cttaaatcaa gaaaggattc ttttatcaag
    20521 cacggttgca agtattcgca taaatcggat tcagtagatt tcacgcgaaa aaaaagagta
    20581 aaagaacaat tgaaaccgtt cttccactct ttttaaactt atgcagttgt ttcttctaaa
    20641 gattccaagt atttatacac ttgttgtcca gcgatcccac catcaccaac agcagtagtg
    20701 atttgtcgca aatctttttc acgaacatct ccgatggcaa aaacgcctgg tatattcgtc
    20761 ttcatttcgt tgtctgttgg aatccagcca gcttcgttcg tcaaccctgc ttctctaaat
    20821 ggtgcagtta atggatccag tccaacatag atgaatactc cgtctgctgc caaatcaaac
    20881 tcattgccgg ttttgacatt acgtgctttc acacctgtca ctaccatttc attcccaacg
    20941 atttcgtcaa cgactgtatc ccacataaag gaaatttttt cattcgcaaa tgcacggtct
    21001 tggatgatct tttgtgcacg taattcatct ctacggtgga cgatgactac ttcagaagca
    21061 aattgtgtca aatagatggc ttcttctact gctgagtctc ctcctccgat cactactaga
    21121 cgtttgttac ggaaaaacgc accatcacaa actgcacagt aagaaacgcc acgtccagca
    21181 aattcttcct cgccaggtac gcctaattta cggtggatac agcctgttgc gataatgact
    21241 gtttttcctt cgtaagtttt atcttcacaa ataacttctt tgtggctgcc gtgatccttg
    21301 atatcttgca cgattccata agcattttct gtcccaaatc ttgaaacacc ttcatacatc
    21361 ttataagcta gatcaggacc catgattgaa tcgaaaccag gatagttttc gatttctgcc
    21421 gtattgttca tttggccgcc aggggcgcct tgttcgatca tcaatacgga taagtttgaa
    21481 cgtgaagcat ataaagcagc agtcatccca gcaggacctg cgccaataac gattacatca
    21541 tacatatgta gattctcctt tacttaatca agtaagcata ctttacagcc aatgcgaaaa
    21601 aaagtctatt gttctgcttg cgtttctcga atagctttat tatactcgaa aaatccgctg
    21661 aaaaacaata gacttttaca aatcattgat caatcattcg aaagattttc tccatttgaa
    21721 tcaattactt ttttatacca gttgaatgat ttcttcttcg aacgggctaa tgtgccattt
    21781 ccttcgttgt cacgatcgac ataaatgaat ccgtaccttt tcttcatctc acctgtacca
    21841 gctgatacta aatcaataca tccccaagtc gtatagccta atagatcgac tccatctaat
    21901 tctactgtat ctttcattgc ctggatatgt gctgccagat agtcgattcg atagtcatct
    21961 tccacataac cattctcatc gggcgtatcg actgcaccta agccgttttc tacaataaat
    22021 agtggttttt gataacgatc ataaatatca ttcatcgtta ttctcaagcc taatggatca
    22081 atttgccaac cccattcact tgcttccaga tatggatttt tgacagaagc aaagatattt
    22141 cctgcagtct gttccaagag ttcgttgtca gtagtcgaaa ctcgagaaga ataataagaa
    22201 aaggagataa aatcaacagt atgttctttc agcaatgcgg catccccagg ttccattttg
    22261 atctgaatgc catttcgttc catttctttc aatgcataag cgggatattc tcctcgtgac
    22321 tggacatcaa tgaagaaata attttctcga tctgattttc tagaagccca aacatcttca
    22381 ggattgcaag tgtatggata atatgctcct gctgccagca tacagcctac tttgttctcc
    22441 ggatctactt catgagcaat cttcgttgcg atggcacttg caaccaactc atgatgtgcc
    22501 gcttgatatt tgatttgttc ttcattttct ccttcttcaa aatacagccc tgcgcccatg
    22561 aatggtgcat gaaggatcat attgatctca ttgaatgtca accaatattt gacaagtcct
    22621 ttatagcgat tgaagatgac tcggcacaga ttttcataaa aaccaacgag ttctctagaa
    22681 cgccacgcac catattcttt gatcaaatgc atcggacaat caaaatgtgt aatcgtcaca
    22741 agcggctcga tcccgtactt ttgacattct ttaaagacat cctcataaaa tttcaaacca
    22801 gattcatttg gctcattttc atcgcccatt ggaaaaatac gactccaagc aatggacatc
    22861 cgataagtct tgaaccccat ttctgcaaac aaagcaatat cttccttata atgatgatac
    22921 atatcgatcg caatttttgc tggataaaaa tgatctttat caaattcaaa cattttcttt
    22981 tttccagtaa tgacaggaaa acggtcttct ccgattggca caacatctac gttcgctaac
    23041 ccacgtccgc cttcatcata accaccctcg cattgattgg ctgctgtagc accgccccat
    23101 aaaaaatctt ttctgaatcc catcatactt cctccattac atgtgtattg cttgacaatt
    23161 ctcttgccag ttcatcttgg acttcgattc cttctgcttt taaccggctg attgtttcct
    23221 tgaattgcgg taaatactgc tgatgtgcaa taaacaactc atccatgatc cgttttgctg
    23281 tctttcctga tcgtataagc ggattgatcg taaacgcttg taaagctgtt ccataatcac
    23341 cagtcacggc tgcttcgatc gtcagcaatt ccatattctt cattacttgc aaccagcctc
    23401 tttcagcagc aggaagggaa ccaaacgcta catttcgtgc gccttgtgcg ccaacatagg
    23461 cagtgacttc tactacacaa tctgccggca gatcaggaac agctccatca ttctttgtag
    23521 acaccacgat ctgtgtttct ttatttccat agatcgaagc aatcgtttca caggcagcat
    23581 ctgaataata agcaccacct cgtttttgca attgttcagg tttatgatcc aattctggat
    23641 ctttgtataa ttcaaacagc tctgcttctg tttctttcac ttgttgcgct cttgtaccaa
    23701 tcgtttgata ttcttctaaa gcatgttcca acatttcttc ttcacggtag taatagcggt
    23761 ggtagccaca agggatcatg ttcatttgtt ttaattgttc tttgaaaaac gggatgtcaa
    23821 agatattttt cggcaatcca ttgtcttcat cgtataaacg atcaatcaaa tctgctgtgc
    23881 ggtcatttcc tttattatct gctactttgt gccaatggaa gtgatttaga ccggcaaact
    23941 tgtagatcag ctcttctttt ttcttgccaa gcattgctgg ttctgtcatc gtcgccatta
    24001 ctggcacatt gcataatccg atcactttat cccatttgcc atatctgata actgcttctg
    24061 tcaccatccc gcttgggttc gcgaaattga tcagccaagc atttggacat aaacgcttca
    24121 tgtcttcaac gattgccaaa accactggaa cggtacgaaa agctttgaat atcccgccag
    24181 caccatttgt ttcttgccca atcatcccat aataactagg gatccgctcg tctttgattc
    24241 gtgcatctaa ttgaccgaca cgaaactgag tcgtaacaaa atccgcatct ttcaatgctt
    24301 cttcccgatc caaagttgca tagatcttga cttcatacgg agaagcatcc cacatacgct
    24361 gagacatctg actgacgatc tcaagctttt cttttccttc ttctacatct acgagccaga
    24421 tttctcgtat cggtaattcc tcatatcttt tgataaatcc ttccatcaac tcgggggtgt
    24481 aactgctgcc tccaccaatc gtagcgattt tgattccttt tgccatgttc tcactccttt
    24541 gctttttaca cttttattat cttttttgta aacaaaacaa acaatagaaa ttcgtttctt
    24601 tccagtaaca tttacaaaag ttagtgtttt ctactggatt tttgtaaata tcttgtcaaa
    24661 aaatgacttt attaagctat ataacataaa tccgtttaca aatcattttc aaaaaagtta
    24721 taatttacgt aaaaaaacta gaagaaggta tctacatgtt attacgtgaa aaaatcaaag
    24781 aaatctcctt ttctccttca gaaaatgaag tgattcagtt tctcttaagc gattctccag
    24841 cacttggcga aatgaccatc caagagattg ctgagcgatg ttatgtccac ccgtccacac
    24901 tcatccgtgt agcaaaaaaa atgggttttg atggctgggt cagttttcgt aatgcttttt
    24961 tagctgaaac agcctatctt gaagggaatt ttcaagacgt agatgcgaat cttccttttt
    25021 ccaccacaga cggtattatg acgatcgcaa ataaaatggc ttctttagaa cagatgacga
    25081 ttcaagatac actttctctc attcatcatg accaactgca aaaagccaaa caactaatgc
    25141 ttcaagcaaa aaaaatcgtg attttcgcca gtaactcgaa tactttgatt tctcaagatt
    25201 tcatgctgaa aatgaagcgc atcaaaatgg atgtcacgat tgtcaccact tttggtgaat
    25261 cgttctatga agcgtacaat tgcacgagtg atatgtgcgg gattctgatt tcttatacgg
    25321 gtgaaaacaa tgtgatcctt cgtaccgcag aaatattgaa acaaaatcat gttccaattt
    25381 tagcgttgac cagtatcggt gattcaacgc tatcctcaat gagtacagcc gctttgcgac
    25441 ttacgacacg cgaacgactg tattcaaaaa ttggtaattt tacgatcaat acatcgattt
    25501 gttatcttct agacgtgtta tatagttgtg tttttgcaga aaattatcaa cagaatttag
    25561 tccatctaat ttctgtagga caaaaatttg ataaacgccc tatcagctca gccatcatga
    25621 aagagccttt caattttgaa aataaagaag tggactaaaa agaatagtta agtctattcc
    25681 attctttcac ctaaccgttc aggtcagtgc ggtgataagt gcatcgtctg gcccgaccgt
    25741 ctccccttga ttttccaaaa tatccaaata atccatgtaa ttggtgacga ttacaggtgt
    25801 ttctaaactg tatccttcct tttcgatttt ctttttgtcg aagcggagca acagatcgcc
    25861 ttgtttcact ctttctcctt gctgcaccaa gctttcaaaa tatttccctt ccaactgaac
    25921 agtatccaat ccgacatgaa tcagcaattc cagacctgtt tcagaaacca ttccgattgc
    25981 atgtttcgtg gggaacaggg tcatgactgt tccgtcaaaa ggggcacgta cttctccttt
    26041 ttcagggtag atcaagattc ctcgacctag tgttccttgt gcaaaagctg catcctttgc
    26101 tgctttcagt ggagcgattt gtccttgtat cggtgttttt acaatttctt tacctgatct
    26161 tttctgtctg tcatctgttt cacctgcttc ctccttccaa aagaaaaaag tcagcgcaaa
    26221 ggataccacc atcgcaatgg cgatcccaat caatacatta atcacatctg ttaaatcacc
    26281 atctggtcta atatagttgg gaattgtaaa gatgcctagt ccaccttgat tccaagaagt
    26341 taaattcatt aacccactat acgcgccccc taacgcacca ccgacacaag taaaaataaa
    26401 tattttttta cgtggtaatg taaacccata aatagctggc tctgtaatcc ccacaatacc
    26461 agaaacaatg gctggtattg caattgcttt gtcttttgga tttctaagct taaagtagag
    26521 tgcggtaagt gctgcagttt gtccaaaaga cactactgca acaggtgtca taatattttg
    26581 ccaaccattc acagaaaatt gcatcaatcc cattggtacg attgcccaat ggagaccaaa
    26641 catcaccaga atttgccaga agaagcctac gattgctcca taaataactg gcgaaagtgc
    26701 taaaagtgtt tcaaatccag ccattaaaat atcagttgcg aatgtgaata ctggtccaac
    26761 gagtaaaaaa cctaaaggca ttgtaataag aacggtgaag aatggaacga agaaattcgc
    26821 gatcatctca ggtactaact ttttaattgg tttctgaatt tggctagcta acaacacaat
    26881 aaagataatg ggaatgacag aagaagcgta gttacgcgca atccagggaa tgccgaatac
    26941 tgtttgataa ataggtgctt caaaaatagt gccagaaaat atggtcgtta gtggtgcttc
    27001 tgctgcacca ctcaaagctt ccagcgacaa gctaggattc attaatgctg cgccaagcaa
    27061 catccctacg aattcattta ctttaaattt acgggcagct gttaaaccga taataacagg
    27121 cataaattgg aatacagaat caccgatatt atcaaacata acaaatgtgc cagaaccgcg
    27181 gtcaaacgcg cctaagaaga caaggaatgc tgtcagtcca cgaatcatcc cgccagcagc
    27241 taagatagca aggaatggtt ggaaacagcc cgatattcca tcaataagca tatcaaacgg
    27301 tctcattttt tctgttgaaa cagcctcatc aaaagtaagg tttcctgctt ttataacctc
    27361 ttcaaacacg actggcacat gattcccgat gacaacttga tactgccctc ccgctcgcat
    27421 taccgttaca ataccgtcca tatttttcaa gatctcatca tttgcttttt cttcgttttt
    27481 taatttgaaa cgaagccttg tgatacaatg tgtcaaacca ttgatatttt ccttcccacc
    27541 gacattttct acgatttttc tcgctaattc ttggtatttt cccatttcta ttccctccaa
    27601 tatattagtg gaaggtttag ccaactgaat gtcactaccc ttatttcata cgttcttgtt
    27661 aatttatcct ccttttttta tttatttaca acgattctca tgcactaaac gcgcagtatg
    27721 gatcgttaaa tacatttttt cgtccgtgga catctggtag ttgaacgtct cggctaaaaa
    27781 gtctgctatt ttctcaacgc aaccaaacgt atccttatat tttttttgaa taacctgaaa
    27841 caattcctca gaaacttctc ccacttctga tttttctcca tctaggacgc gtgtcagaaa
    27901 aaaacgtaag tgtgtgacga atcgaaaaaa atggactgaa tcttcatcaa atgtaatctt
    27961 atagtaatag cggacgatat ttagaatatc ctgtattgtt atagtaaaca ggtaggtatt
    28021 gttttgttgg ctttgaatgc cttgtgcatt gactaaatgc aatgcgataa atccggcttc
    28081 atcttctgat aacttgatgt ggtatttatc cgagattttc ttcactgttt ttttcccgat
    28141 ggcatactct ttaggaaata gttgtttgat atcatacagc aatacatttg gtacaaatat
    28201 tcccattttg tgacgttcga tagccgtata caaatgatcc gtcaatgcaa tatacacaga
    28261 atcattcaac tggacttgta gagcctcttt tgtatattca atgatttcac aagaaatttc
    28321 taagtactca agaggaatcg cttgtgccaa ttcttggaac tgttgtgaaa gcccgtcatt
    28381 tgtcaactga aattttttgt cgatgcatga gtcaaaaatc gggtcaccca ctttcttttt
    28441 gaatgccaaa ccttttccca tcacgatcat ttcctgattt ctttcatccc tggttattac
    28501 gacgttgtta ttcagtactt tctcaatcag catgttttgc tcctttccat gatatatagg
    28561 gctgtattca aaacaaaaag aaaaacccaa aaagccgtcc atggaaccat gggagactca
    28621 tcaggtttag cttgcatcag gcaatcacta tccaagttgt atcgttttct ttatattatc
    28681 gaaagcaaac gcttttgtca acgctttctt agtggctatt aggcactttt gactagctgt
    28741 cgtcgtcttt tttaaatcgg gtgactacgc cactaacgct cacagatgaa gaatctggga
    28801 agctctgttt tattttcact ctagcgctta ggacaaaatg ctactatgct actgtcacaa
    28861 cctcttttta cacaccactg ctttgcagtt tgtacatatc tgcatacaaa ccattttgcg
    28921 ctagaagtgt ttcatgattc ccacgctcta ctatatgacc tttgtctaaa actaaaatca
    28981 attctgcatc ccgaatcgtg gacagccgat gggcaatcgc aatcgttgtc cgtccttgtc
    29041 gcatttttgc caatccttct tggatcaatc cttcggtttc agtatcgata tttgctgttg
    29101 cttcatccaa tactagtatt ttaggatctg tcacgattgt tcgcgcaaaa gtgatcagct
    29161 gtcgttgtcc actagaatag ctggcaccgt gttcgatcac ttttgagtga tatccttttg
    29221 gtaattcatc gatgaacttg tttgcttgaa caaactcagc tgcatggcga atctgttcgt
    29281 ctgtgatagc tggattcatc atccggatat ttcctgcaat atctccataa aacaagaagg
    29341 catcttgcaa gaccaatccc attttcttac gtaactcttc cattggatat tctcgaatgt
    29401 catgatcatc aatcaagatt tggccttcat aaaattcgta aaaccgcatc aatacgttga
    29461 tgatcgagct ttttccgctt ccggtgtgcc ccactaatgc aacggtctca cccggattag
    29521 cgacaaaaga gatattgttc aatacattgt gctctccatc ataagaaaag cttacattgc
    29581 gaaactcgat cttacctgaa gtaatcgctt gatctgctcc aggattttgt tgcggtgcat
    29641 attcttctgt atccatgatt ttcaaaatac gactaccggc aaccatccca tctgtaaagg
    29701 tgctcaaaaa gtccatcatt tgagacattg gattaaaaaa tccctggaca taggtcgtaa
    29761 atgcatagat cagtccaact tctacgggag aatgcaacgc atcgatccca aacaaagtca
    29821 acgccaacgc aatcgccaat gcatagagaa aattgatgat cggacttaac agaatcgaat
    29881 tcgttttgat catcgcatag cgcgtgtcta aatactcgtt gttcacttgt tcaaattctt
    29941 tagctaaacg agcttcttgg cgaaactgct ggataatacg catgccggaa atcgattcat
    30001 tcaatttcgt gttcagctga cttaattttt cccgcatatt tcggtaaata cgagaactga
    30061 atttctggta ataccagatg acgattccta aaatcggcaa gaacaccaaa gtccacaagg
    30121 caatttctcc attgatttga aacatggcaa cgaaagaaga aacgactgca aagatcccag
    30181 tcaataccat caaaaagaca taccaaaatt caaatagcgt ttctgtgtca ttcgtcactc
    30241 gagaaacggt cgatcctgcc ggtgtctggt caaaataacg catacctagt gtatgcagct
    30301 tttcaaataa tcttacccga acatactgat aggtcttcaa tgaagccatg gaatacaaat
    30361 accattggaa aaaccaaatg atacttttga ctatgactcc aaacaagtac acaccagcaa
    30421 aaaacaaaat aacttgtgtc gtagctgact gtttagccag atgatcatcc ataaaagttt
    30481 ggatgatcct tggcaatagg acattgatca cagacagggc aaacgcaaat aagatagcaa
    30541 cgatgaacgt tcttctaaaa ggtttggcga acttcatcag tcgtttgatg atcttcttct
    30601 gttctttaag tggaatcgat tttgtccatt ccgattgata attttcatcc atcaggcctc
    30661 gcctccttct atcttcgctt ctaattgctg tttttcccac attcgttcat accagccacc
    30721 taatgcaagc agctcgtggt gggttcctcg ttcaatgatt ttcccttgat cgacaacaat
    30781 gatttctttt gcatgcatca cgctgcttaa acggtgagct gcaatgatgg ttgttttctc
    30841 ttttcttatt tcttttaaat tatgcaagat agcttcttct gttttggcat ctactgcaga
    30901 aagtgcatca tctaagatca gcaattcagg atttttgatc aatgctcgag caatcgaaat
    30961 cctttgtttc tgtccgccag ataaagagac tccacgttct ccaactaagg tatcataacc
    31021 ttttggcaat tccttgattt cattgttgat aaatgctaaa gaagccgcat gctctacttc
    31081 ttcttctgaa agttttggat tagaaaaacg gatattatct cgaatggtca tcgaaaacag
    31141 gaagtgatcc tgaggcacat aaccgattga tcctaataaa gcattcaatg aatattcttt
    31201 gatcgaacgt ccgccaaatt ggattttccc ttgataatca tcgtattcac gcatcagtaa
    31261 tttgatgatt gtcgtttttc ccgcaccagt tttcccaaca atacctaatg tttctccttc
    31321 ttggatcgca aatttgattt gttctaatgc tggtgattct tctttcggat agttaaaaga
    31381 ggaaatagct acattcagct cgccttttgc cggggtctgc actgcaccca gttgttccac
    31441 gatatgcgtc ttttcatgca aaagttcatt aacgcgatca taagaagcat ttcctctttc
    31501 caatacgttg aacaatcggc caatcgcaaa catcggccaa acaagcatac cgatataact
    31561 aataaatgag accatctgcc caatcgagat cgtgccttcc aagacatact ttccacctaa
    31621 aataattgtt gcaacataag acagaccgat aatcaaggta atgaatggat caaataagga
    31681 gtctaaaaaa ttgactcgtt tgtttttaat aattgcatct tcaatctttt cttcaaaatc
    31741 cgcgagatct tctttttctt gtccaaatgt tttaatgact ttgatcccag tgatactttc
    31801 ttgtgtctta tcattgatac tagaaaaagc ttcttgcgaa tcacgaaaag catcgtggag
    31861 ttttgacccc agcactcgtg aagtaacggc tagcaaaggc agaggaatca gcgcaatcag
    31921 tgttaagcgc caatcaataa acaaaatcat cgcaatgatc gttgttccgc ccgtaatcac
    31981 tgaatctgca aaggtcaaga ttccagctcc tgctacattc tggattgcat tcaaatcatt
    32041 cgttgcatga gccatcaaat ctccagtccg atatttttgg taaaagagat tgtccatttc
    32101 tgtaaaatgt ttgaatagct gctgccgaag ttctttctcc aaccgagctg cacttcccca
    32161 aatattcgtc cgccagatat aacgaaaaag atattgtaag aaagctgcaa ggatcaatag
    32221 taagatccac caagaaatga tctttattga taaattctta tcggcgatct catcaatgat
    32281 gatcccaatc acttttggag gaactaactg taccaaggcc actgcgaata gggagaaaat
    32341 cccaattaaa taatgtttct tttcctgacg aaaaaaccaa cctaattttc taaaaatcga
    32401 cattttactc ctcctttgtc tttatcaaga gcataggatt gtctgtcctt gtcttttttg
    32461 ctgaatagtc tgaatagtct aacaaattac gaaataaaaa acggactgaa aaaatcagcc
    32521 cgcaaaaagt ctttgggtca gaacctattt tttcccttgg ttcttcattt gctgcatcat
    32581 ctggcgaatt ttcttctcag atggtttttg ccccattgac atcatcattg atcgcaacat
    32641 gtcctcatta acgggaggat ttttttctaa atagtctttc atatatttac gcgctaggaa
    32701 aaagccacct actgcgccga ttagcaaagc aataatagca ataagtacga caatacctgt
    32761 tgataccata taaatctctc ctttctattt ccgaattgct ctcaatgatt tattttacta
    32821 aagtttattg aaaaaagaaa ggcgtattct ttagtttcat gcaaaaaata gtggtttttt
    32881 tagagaagaa caaaatttct ttcattaacc agcaagacac gccacgaaaa aaaattaggc
    32941 ataccgaatc tttttcttta attttttctt tctgaggtgt agaatgtaat cgtagataaa
    33001 taggaaaggt ggttttttct tgaataatca atccaacaaa caatttcata agcatcgact
    33061 tattgaacat acaaatggtt tatctttagc tgaagtaaat ggaacagtgg atgtaccaaa
    33121 gggaaaaagt ttttttaaaa cacttcttgc ttactctgga ccaggtgcat tagtagcagt
    33181 tggttatatg gatccaggta actggtccac ttcgatcact ggtggacaaa atttccaata
    33241 tttattgatg tcagttattt tgatgtctag tctaattgct atgcttttac agtatatggc
    33301 agcaaaatta ggaatcgtga cccaaatgga tttggcccaa gcaatccgag cacggacaag
    33361 taagacattg ggtgtcattt tatggatctt gactgaatta gccattatgg caacagacat
    33421 cgcagaagtc attggtgcag caatcgccct ctacttactt tttgatattc cattggtaat
    33481 tgccgtattt atcactgtgt tagatgtttt cgtcctttta ttgctgacaa aagtcgggtt
    33541 tcgtaaaatc gaagctttgg ttgtctgctt gattttcgtc attttattcg ttttcgtcta
    33601 tcaagttggc ttgtctaacc ctgattggca cagtttattt gctggcttta tccctaacag
    33661 tcatacattc tcagactcac ctgtcatagg tggtgaaact ccgctgactg gagcgttagg
    33721 gattattggt gcaacagtca tgccgcataa cttatatctt cattcagcag tttctcaaac
    33781 tcggaagatc gatcaccaag acgaagaaga tgtagcacaa gcggtacgct tttctacttg
    33841 ggactctaat atccagttgt caatggcttt tttagtcaat gcactgctat tgatcatggg
    33901 tgtcgctgta ttcaaaaccg gttctgtcca agatccttcc ttttttggtc tgtatgaagc
    33961 tttatctgat ccatcagcta tgagcaatgg tatcttgatt gctgtagcaa aatctggtat
    34021 cttgtctact ttatttgctg tagcacttct tgcttctggc cagaactcta ctattactgg
    34081 tacgttgacc ggtcaagtca tcatggaagg attcatccat atgcgtatcc cgatttggct
    34141 aagaagacta gtcacacgat tgctttctgt gattcctgta ttgatttgtg ttttgtttac
    34201 aagtacaaaa ggaacgatcg aagaacatac cgctttgaat aacttgatga atgaatccca
    34261 agtttttctg gcttttgcat tgccattctc gatgattcct ttactgatga tgaccaatag
    34321 ccaagcggag atggggagcc gattcaaaaa ttcatttgtt gtaaaacttc ttggctggtt
    34381 ctctgtgatt agtctgactt acttgaactt acgaggatta ccaggtcaaa tcgaaagttt
    34441 cttcggcgag caagccacct cagctcaact agcattggct gatcaaatcg cctatgtaat
    34501 cattgttctt gtccttctcc tgttggcatg gacgattggt gatttatata aaggaaacaa
    34561 aaaatatgaa cagcgtttgc aagaaacgat agaagaaatc gaatagaaac tgattctttt
    34621 acacaaagga ggttgtgaca gaagtgggca gcttcaagaa ataagaagga atttaagaaa
    34681 attgcttttc aaatttttgt gaaatttcgg cttatttccg aagaagttac ttctggaaca
    34741 ccgtgtat
//
LOCUS       DACKAC010000023        34660 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.023, whole genome shotgun sequence.
ACCESSION   DACKAC010000023 DACKAC010000000
VERSION     DACKAC010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.023"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(313..2205)
                     /locus_tag="I0B46_001185"
     CDS             complement(313..2205)
                     /locus_tag="I0B46_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAR2083499.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2202..3929)
                     /locus_tag="I0B46_001186"
     CDS             complement(2202..3929)
                     /locus_tag="I0B46_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAR2083500.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3945..4595)
                     /locus_tag="I0B46_001187"
     CDS             complement(3945..4595)
                     /locus_tag="I0B46_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAR2083501.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4799..5095
                     /locus_tag="I0B46_001188"
     CDS             4799..5095
                     /locus_tag="I0B46_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAR2083502.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5287..5907
                     /locus_tag="I0B46_001189"
     CDS             5287..5907
                     /locus_tag="I0B46_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAR2083503.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5999..7165)
                     /locus_tag="I0B46_001190"
     CDS             complement(5999..7165)
                     /locus_tag="I0B46_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAR2083504.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7177..7530)
                     /locus_tag="I0B46_001191"
     CDS             complement(7177..7530)
                     /locus_tag="I0B46_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAR2083505.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7658..8680
                     /locus_tag="I0B46_001192"
     CDS             7658..8680
                     /locus_tag="I0B46_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAR2083506.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(9119..9553)
                     /locus_tag="I0B46_001193"
     CDS             complement(9119..9553)
                     /locus_tag="I0B46_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083507.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9663..11522)
                     /locus_tag="I0B46_001194"
     CDS             complement(9663..11522)
                     /locus_tag="I0B46_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAR2083508.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11640..12799)
                     /locus_tag="I0B46_001195"
                     /pseudo
     CDS             complement(11640..12799)
                     /locus_tag="I0B46_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
     gene            complement(13106..14530)
                     /gene="araA"
                     /locus_tag="I0B46_001196"
     CDS             complement(13106..14530)
                     /gene="araA"
                     /locus_tag="I0B46_001196"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAR2083509.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14546..15244)
                     /locus_tag="I0B46_001197"
     CDS             complement(14546..15244)
                     /locus_tag="I0B46_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAR2083510.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15201..16862)
                     /locus_tag="I0B46_001198"
     CDS             complement(15201..16862)
                     /locus_tag="I0B46_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAR2083511.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            17080..18480
                     /locus_tag="I0B46_001199"
     CDS             17080..18480
                     /locus_tag="I0B46_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAR2083512.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18732..19811)
                     /locus_tag="I0B46_001200"
     CDS             complement(18732..19811)
                     /locus_tag="I0B46_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAR2083513.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19897..20856)
                     /locus_tag="I0B46_001201"
     CDS             complement(19897..20856)
                     /locus_tag="I0B46_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAR2083514.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDTLIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20868..22390)
                     /locus_tag="I0B46_001202"
                     /pseudo
     CDS             complement(20868..22390)
                     /locus_tag="I0B46_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
     gene            complement(22400..22963)
                     /locus_tag="I0B46_001203"
     CDS             complement(22400..22963)
                     /locus_tag="I0B46_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAR2083515.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22969..23832)
                     /locus_tag="I0B46_001204"
     CDS             complement(22969..23832)
                     /locus_tag="I0B46_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAR2083516.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23846..24742)
                     /locus_tag="I0B46_001205"
     CDS             complement(23846..24742)
                     /locus_tag="I0B46_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAR2083517.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24917..26188)
                     /locus_tag="I0B46_001206"
     CDS             complement(24917..26188)
                     /locus_tag="I0B46_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAR2083518.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26178..27695)
                     /locus_tag="I0B46_001207"
     CDS             complement(26178..27695)
                     /locus_tag="I0B46_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAR2083519.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            27893..28783
                     /locus_tag="I0B46_001208"
     CDS             27893..28783
                     /locus_tag="I0B46_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAR2083520.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(28856..29680)
                     /gene="nadE"
                     /locus_tag="I0B46_001209"
     CDS             complement(28856..29680)
                     /gene="nadE"
                     /locus_tag="I0B46_001209"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAR2083521.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29692..31164)
                     /locus_tag="I0B46_001210"
     CDS             complement(29692..31164)
                     /locus_tag="I0B46_001210"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAR2083522.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(31801..32163)
                     /locus_tag="I0B46_001211"
     CDS             complement(31801..32163)
                     /locus_tag="I0B46_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAR2083523.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            32386..33138
                     /locus_tag="I0B46_001212"
     CDS             32386..33138
                     /locus_tag="I0B46_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAR2083524.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(33278..34201)
                     /locus_tag="I0B46_001213"
     CDS             complement(33278..34201)
                     /locus_tag="I0B46_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAR2083525.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(34216..34488)
                     /locus_tag="I0B46_001214"
     CDS             complement(34216..34488)
                     /locus_tag="I0B46_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083526.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        10229 a         7212 c         5981 g        11238 t
ORIGIN      
        1 caagaggttg tgagcctgcc gtttaactcc gagtcacaag gagcaatttc aaaaaacagc
       61 ttctcaggtt tgttgactat tgtgacttgt gaccgaggag ttggcaggag aacaccgttt
      121 aaggaagagg ttgtaaaatc agcggtttga cctgagtatt agagatacaa aacggaaaaa
      181 tagcttctca tattttgccg ttttttgagc taataccgaa ggacagctga ttgaacaccg
      241 tttattcgga aaaagggact gtgacacgac ttttgtcaca gcccctaaaa aaaataataa
      301 atttgtacta ttctatgaag cttcttcttc agaaaactga ctgttataaa gatctgcata
      361 gaatccgtct ttttccatca atgtatcgtg gtttcctgtt tctacgatcg aaccatgatt
      421 catcacaata atgttgtctg catcgcgtat tgtagacaag cggtgagcaa caacgaaact
      481 tgtccggttt tccaataggc ggttcatcgc tttttgaatc agtatttctg ttcgtgtatc
      541 tacactagat gttgcttcat caaggatcag gacatctgga tttgctaaga atgcccgcgc
      601 aattgtgatt agctgacgct gcccttgcga aatattgctt gcatcctcgt ttaatacagt
      661 gttatatcct tctggcaatt tacgaacaaa atcgtctacg tgagcagctt tagcagcctc
      721 aatgatttgt tcgtctgttg catcgtcgtt cccatatttg atattgtcat agatacttcc
      781 tgtaaacagc caagtatctt gcagtaccat tgaaaactgt gcgcgcaatt catcacgtga
      841 aagatcacgg gtatctacac catcgtaacg aatactacct ccagaaacat cgtagaaccg
      901 ttcaagaagg ttgatcagtg tcgtcttccc tgcaccagtc ggacctacaa tagcgaccat
      961 ctcaccaggt ttgacatcca agttaaaatc agtcattagc aagttatctg gtgtgtagcc
     1021 gaattgaaca ttttcaaaac gaactttgta tggtgtatct ttttcttctg gaaggccaga
     1081 tttttcattg gacatctctt cttcatccaa cacttcaaat acccgttcgg ctgaagcaac
     1141 tgttgattgg atagtgttca tcaaacttgc aagttgagtg attggttgag agaactgtgt
     1201 tgtatattgc aagaacgctt gaacatcccc taatgtcatg ttcccatttg caacttttac
     1261 tccgcctagt acagcgacga acacatagcc tagattttta acgaaattca tcaacggcat
     1321 gatgattgcc gaaataaatt gagctttcca accagcggca tacagattct gattttcttt
     1381 ttcaaatact tcctggtctt tttcagtatg attgaatgtt ttaacaatgg tatgaccacc
     1441 atatgtttct tctacttggt tattcaataa acctaaactt ttttgctgat ctgcgaaaaa
     1501 cacttgtgat ttcggcgcta cgatcatcac gatgatcaag cttaaaggga tcatcaataa
     1561 cgcgactaac gtcaattgcc agctgattga aagcatcatc acgatgaccc caatcaatgt
     1621 gactgcacta gttacaaatt gagtcaaatt ttgctgcagc gtgcttgcga tgttatccat
     1681 atcattgatt gctcgagaca tgatatcccc atttgtatgt gtatcataat aggaaatcgg
     1741 tacacgattc attttggctt ccaaatcatg acgtaattca taaactgttc gttgggaaac
     1801 acgtgtcata ataaattgct gtaaaaagtt gaatacagca gaaatcaaat acatcacgat
     1861 tactgtcgca atgatctgcg cgattttatc aaagtctatc ggaaattttg ttacttgttg
     1921 gccttgttgc atctgttgag cgccagccat tacacctttg aatatttctg tggttgcttt
     1981 ccccaagacc tttggtgttt ggatttggaa gatcacggct gctattgcta gtacaaatac
     2041 agcgatgatc gcaaccatac gtttagacat atagccaaac aatcggcgta ccgttcccca
     2101 gaaattttta ggcttttcac cttttacacc gagatttctg ccaggtccac catgtcctgg
     2161 accgcctgct ggtccatgag agggacgtgt gtttgtttta ctcatgctaa gtcctcctct
     2221 cttagttgtg aatgaacgat ttcttgataa gtttcgtttg ttgctaatag ttcttcatgt
     2281 gttcctttgc caaccagttt tccttcatct agcacaagga tcatatctgc atccattact
     2341 gtacttactc gttgagcaac taaaacggtg atactttctt tcatattcgt tttcaatgct
     2401 tggcgaagat tggcatccgt tttgaaatcc aacgctgaga aggaatcatc aaacacatag
     2461 atatctgctg ttttgactaa tgcccgtgca attgccagac gctgtctttg tccaccagag
     2521 aagtttcctc ctccttgttc cacatggcta tctaagccat cttccatctc tgaaacaaaa
     2581 tctttagctt gtgcgatttc taatgctttc caaatcattt catctgttgc atccggctta
     2641 ccatactgca tattgtctcg gatcgttcca gagaacagaa ctgctttttg aggaacaaag
     2701 cccattaatt ctcgcaaatt atattgagtc atgtcactaa tgtccgttcc atttatttgg
     2761 atcgaaccag attcgatgtc atataatcga gggatcaaat tgactaaggt cgttttacct
     2821 gaaccagtcc cccctatgat tgcgacgatc tcgcccgact tggcttggaa atcaatatct
     2881 tccaatgcaa gtttttctgc tccatgatat cgataattga catgattgaa ggccaatgtt
     2941 gcgttttttc cagcaaaaga aactgtttta ggattttcag ggtcatggat accgattttt
     3001 tcatccaaca cttcattaat acggtcagct gaagcttgtg cacgaggcac catgataaaa
     3061 atagcggaaa gcatcataaa gctgatcaag atctgcatcg cataagtcat aaatgcaatc
     3121 agattaccga cttccaaagt catatctgct atgtagtgtc caccaaacca agtgattgct
     3181 acgtttgtac cgctcatgat caatgtcatc attggcatca tcaatgcgac gatcgtatta
     3241 actttgattg ccgtatttgt ataatcttta ttcgctaaat caaaacgatt ttcttcaaag
     3301 cgtgtcttat cgaatgctcg aatcactctg acccctgtca atccttctcg aaataccagg
     3361 tttaaacggt cagttttctt ttgcatactt ttgaataatg gaacagcaaa gtacatgatt
     3421 ccgccgatca aaataatcat tactgggatg acaactaaga agatttttgt caactgatga
     3481 tctttttgat aagcaagtat gctcgcgcca atcaacgtga ttggtgcatt gatcatcata
     3541 cgtaaaaaca tttgtgtaac catttggatt tgagtcacat cattggtcgt tcttgtgatc
     3601 aaagaggccg ttccatattt atcaaacgca tcttttgtca gactctctga ttttttatag
     3661 acatccgtac gcaattgctt acccagtttc tgtgattcac gagtagcaaa aaatgtattt
     3721 gcgatagcag ctaagatact gatcaaagaa aagccgatca tcacaaaacc tgtatgccaa
     3781 atataatcga catctccttg cgctacccct ttatcaataa tattggaggt aagtgtcggc
     3841 aaatataaat ctgccagtac ttggatcacc atgaaaactg cagctgcaat tgctgaagtc
     3901 aaggagatcc gttttacgat ttttagcatc tagttccctt ctttctattt atatactccg
     3961 tttttcagcc aagaaagttt caaattgaat tcactgattg cttgctctac tgagtaatct
     4021 tcagatgttg aaaataagcg atacgaatgc gcaattgttg taaatacagt attcatcact
     4081 aattggatca gtaattccaa ttcatgatca ttttttactt tcagcttcgt ccgatcgatc
     4141 aattcaagca actcttttgc atcttgtttg tgttctttat gtggatcgtg aggcgttttt
     4201 tggcgactcg tatgaggagc gacttttctt gaggcacggt aatccatgtt cataaacaag
     4261 ttacgataaa aagaagcatt ctcgccttcc aaaatctcat aaatcatttt agaaaaatag
     4321 gattcgaatc ctttgaatag atcaccatcg gattctttca ttagttgtaa catatcttta
     4381 gagctgtcac gtctcattgt ctcaaagtaa taaaaataaa gatcctcttt atcttcaaaa
     4441 tactgataga aactgcctct agggatatcc gctaatttga cgatattagc aatcgaagct
     4501 tcttttaagg gtactctaga aaactcgatt ctcgcagcct cgattagtct tttttgtttt
     4561 tctttaggca aatggaaaaa tgtctgtgtc ggcatatgga ttctcctttc tttcaagatg
     4621 acacattgtc atatgacgct atgtcattat aacacctcct acctgttttg caagacattt
     4681 gcatagtttt tttcattatc atccgataca ttaaaaaagt gataagagga atccttccat
     4741 ttgaattaat gagagttgcc cattaaaata gccacatact aaacaaagga tgagataaat
     4801 ggcccaatca attagtgtcc aagacttcaa taaattgcct ctcgatgctg acagcatcgt
     4861 tttagataca agaaatacct tagattacac aaaaaaccac ttaagccact cgctttctat
     4921 ccccgctgct atcttgccaa gacaattaaa aaatctagat ccaaagaaac tttattatat
     4981 tctaagctat acaggcaggc gatcagaagc attagccgat caacttactg ctaatggttt
     5041 tcgtgctgtt tatatcattg gtgggatgga agctcttcag tcgagtgttg cttgaatatt
     5101 gcttgttaga ttcaaaagag gctgtgacaa aaaatcgtgt cacagcctct tttataccct
     5161 gtcttttttt agttatttat tcttcaacct tatcaatatg acttgatagc cttttctttt
     5221 tcgagtaaac tgattttgtt agtcatagtt ataacaatca tagcaataaa aggagaaggt
     5281 aagcatatgg catctgtttt agtagtcaaa ggtcatcccc taactgcaga agaatcgcgc
     5341 acagttaagg cattgacgtc ttttttaaca agttataaag aaaatcatcc agatgatgaa
     5401 gtgactgttt tggaacttta tcgcgatgat attccggaaa tcgatgaaga attattatca
     5461 ggttgggaag cattacgtgc tggtgcagaa ttcacttcct tatcagaaag tcaacaacaa
     5521 aaaattgctc gtttcaatga attgactgat cagttccttg ctgctgataa aatcgttatc
     5581 gcaaacgcct tatggaatct aaatatccca acaaaattaa aagcttggtt cgataccgtc
     5641 aatgtagctg gaaaaacgtt ccgctataca gaaaacgggc cagaaggatt agtgacaggt
     5701 aaaaaagcac tgcacatcca atctaatggc ggtgtataca acggacaaga ctttgcctct
     5761 caatatgtaa aaggtatttt aaactttgta ggcatcgatc aagtagatca gctattcatc
     5821 gaaggaatcg attatgaccc tgaccgtgca gatgagttga tgcaaaatgc attagataaa
     5881 gctgctgctt taggtaaatc attttaaaaa ttgagataaa tgcatacaaa agagacagag
     5941 gaaaaaatct tcaaagcagc atttgtgcct tggatatttt cccatctgtc tctttttgtt
     6001 attctttctg ctttttcaac ggattttctt cataaagaga agagattgtt gtgtaccatt
     6061 caaaaacatg agtaatcact actttagctg ctgcataaac aggaatcccc aagatcacac
     6121 cgatgattcc gaacaatttc ccagaagtca gcagaactaa taaaatcgtt actggatgga
     6181 ttgctaactg attccctagt actaatggag agatcaagcg tccttctatt gtctgctcta
     6241 ctgcaaaaac gatcagtact ttgatgatca tgaatggacc tgctacaatg gctaaaaaga
     6301 tagccggaat catcgctaaa aatgatccta agtaagggat caggtttaaa aaacccgcaa
     6361 taatccccaa cgtgacagca tactctaaac caatgaccgc aaaaccaatc ataaacatga
     6421 ttgccacagc aaaagccaca gttagttgtc ctcttatata agaagaaact tgactattca
     6481 tttcactcag cacattcaac gtcggttttt gcatgcgtgt cggtaaaaat ttgacaaaat
     6541 aaggtgctaa ttgtttaccg tctttcaata gataaaataa gataaacggc atcgtcagca
     6601 gagccactaa aatagtcgct accgcgccga caaaacttcc aagactctga actgtactct
     6661 tagatatatc ttggatcata tcgcctgccg aattcatgaa cttatcacct gctgtttcca
     6721 actgttcgcg aaattggttg aacagcgggt cccgcaaaat ctcggttaat ttattatcaa
     6781 tcgtatctac atattgaggg aagttactga tgaagctcac tgtttgttcc tgaatcttag
     6841 ggatgatcac tacgctcccc catgcgatta gtgcaacaac caaaacaaac agcccaatga
     6901 tactgtaaag ccttggtact tttcgctttt caaagtaatc aactaccgga ttcatcaagt
     6961 aatacaagat tcctgctaaa atgataggca gacctacgat tgctaaaaat tgccagattg
     7021 gtgcaaacaa atatgaaact tttgtaaata aaaagacgat cagcaatatt aacaatacaa
     7081 caagtaaggc tgtgactact tgattattta aaaaccagcg ccaaaaccac gataccggtc
     7141 tgttcttctt attttttcct tccataattc ctcctgttag ttataaaaaa tttggcttcc
     7201 caaaccaata gcaggcagtc tatgcggatg aagatagatt ccatttgcaa tttgtccttc
     7261 ttctggacaa gcatcaaaaa tcaatgatac gtgagctact gtctttaaat tcccattggc
     7321 catcggaccg acataatcga tcgtatatac ctgatcatca aaagcaattg ttccgcctgc
     7381 tttcaaagaa aaaggttcgt cttttgatac ttcttgaatc accgaatatt ttcgtaaggc
     7441 agctgttgct gttttatcga acaaaatcag catgggttct tccttgttta ttgcttctgt
     7501 cccaatttct gtaatctttg cctgaatcat tgcactctct cctttaattg ctttcaaaaa
     7561 cagtataaca cattctcatt ctttagccgt gtggaataat tatgtgatag aataaaaacg
     7621 aaaactattt cattacaatt aggaggaatc agctcatatg atcgaaaaga taattttagg
     7681 aacctatacg cgtcgcgaaa gtaaaggaat ctatacgatc gaccttgata cagaaaaaga
     7741 agaattagcc aacctttcat tagctgttga agaaaatagc cctacctatg tagcaaaaag
     7801 taaagctggc aatctttaca cagttactag tgtggatgga ttaggtggtg ccggtgcgta
     7861 cgataaggat tttcattttt tgaatgcggt aacagagtct ggcgctcctt tgtgttatgt
     7921 agcggttgat gaagcacgtc aattactata cggggcgaac tatcataaag gtgaagtaaa
     7981 tgtctaccat atattaggag atggcgaact gaaagcagca gattcaatat tccatcaaga
     8041 agcgacaggt cctcataaaa accaagatca tgcgcatgtc cactatacgg atctgactcc
     8101 tgaccaacga ttagtcgtat gtgatcttgg tacagatcgt gtgtatactt atgatgtgtc
     8161 cgaaaacggc aaattgaatc ttgtaactac cttcactgca gagccaggaa caggccctcg
     8221 ccatcttgtc tttcatccaa atggacaatt cgcttattta tttggtgaat tagacagcac
     8281 agtccgcgtg ctttcttata ataaagaaac aggtgctttt gaagaattac agaaaatatc
     8341 gaccttgcca gaagaattcg acggtgaaaa tggaggggca gctatccgca tttcaaatga
     8401 tggcaagttc ctttatgctt ctaaccgagg acataactcg attgctgtat tcgcagtttt
     8461 tgaagatggt tcccacattc aaaacaagca aatcatctca acagaagggg attttccccg
     8521 cgatttcgca ctagatccaa gcaataattt tgtcgtgtgt gccaatcaga acaccgataa
     8581 tctgacatta tatcgccgtc atcctgaaac aggattactg gaattactga aaaaagatgt
     8641 ctatgtacca gaatgtgtct gtgtttattt tgatcgttag taagaaaagg agaatgaaat
     8701 gaatcagcaa gacgtattag acagactacg ggaagaacta aatattcctt tctttgatgg
     8761 gaaaatcgaa gataaagagt atagcgaaga ggaatatcag aaactcaaag ccgatttaca
     8821 gaactacttt gaacaatatg tacagaatgt agaaaactaa tcagctttcc atactatctg
     8881 aaaagaacat caggacaaga cttttccgtc tatcctgatg ttcttttcta caaataaaaa
     8941 accgccgaga tggttagact cgacggaaaa ggagtattta ctcgattttg agtaagtaca
     9001 aggttgttgt tggttatagg tatgtcatta tcataaagga ttttgatcac tctatcataa
     9061 aggtaatatt acatttatat tagataactc tagtttttat tgttatcaaa gatttctttt
     9121 attttttcaa caacaaatta gtcatttttt ggatggcagc tgtcaattgg tctgtcgctt
     9181 ggtcttgttt tgcttcagat gtattgaata gatcttcatc tacccctgat tcaagatcga
     9241 tactttggat catttcttca cagcctgcta catatgaaag ataagctttt tcaaaatttt
     9301 tatgaatgcc cattgctttt gctggtggac gcaaagtact gactttcata agcattgccc
     9361 gatatttttt tgttccttct tcaaataaca ggatgatctc ttcaagagtc gatttgtcca
     9421 gttcacttgt ttttccttca tcaattgcgt ttctgatcac ttcatatttc ggatgcattc
     9481 cttctccgat tgcttctgtt tccttgacga tttgatcgat ggactgcaca taatagccaa
     9541 tattcggtaa catgttcgtt ttcctacttt ctccattgtt gttattattt attctacttg
     9601 tttttatcct caaatgacaa gtacttgaaa aaatttaatg gtttctatac agctcgccat
     9661 tcttattcgt gctcaaagaa accatctgct aaactatatt gtttccctgc gacaatgtca
     9721 tattggatca attgatattc gtcgtaaatc tcttgttgcg agcgattgaa ttgcgcttct
     9781 ttataccact gtccgttttg atagtacagt tctcgttcaa acgccggaag ttcctgttct
     9841 aatgataaaa gcagctgata aaagccattt gttgtctgat tcgtctgttc cattagattc
     9901 gctgcaaaat aaaacggact ggtgattgca tcgtgagtcg tttgcttttc taattctcct
     9961 ttactgtcga acataagaaa ttgtgtttca tgcaatgttt gtttgtcatt ttttgcttgg
    10021 atcgtatctg aatagatccc tggaagatga tccccccaaa aaacaactaa agtccttcta
    10081 gaaagattct tcaattcttc cgtgaactgc ttcagtgccg tgctgctata ggaaatatct
    10141 tgcagataat tctctaagtc aagtgtccca cttccatctg aagtttttgc aggatagtct
    10201 agattatcat actttccatc gtagggcata tgtgtctgca tcgtgactaa atgaatgaac
    10261 tgaggtgttt tttcttcctt gagaagtgtc attacttctt tgtaagctga ctcatcagaa
    10321 atatatggat tattctctat cgtatccgta taggtcatcg tacgctcgct gatgaactgg
    10381 tcaaatccta atgtctgata aacatcctct cttttataca tcgaagtatt gtatggatgg
    10441 atcgctgtcg tctggtatga ttgtgcgttt aacgctgaaa cgatagaagg aagctgatcc
    10501 atttttggta caagcatcgt gtaaggggtg gtgagttgcg cattaaataa cgccatagaa
    10561 aaaccagtca atgcttcaaa ttcgatattt gctgttccac cgccatagtt ttgagaaagc
    10621 atattgccac tatatgtttg atcagctact tcataataat ctgccaatgg ctcaccagat
    10681 acttctacgc cgtttaatcg actaggatct gagaaacttt cactcatcac gaacacgatg
    10741 ttcggcgact cttcctctac agttttattt ttttcatcgg ctagcttctg gtatttttca
    10801 gtgatttctt tgatcttttc tttcgaatat ccttcaggtt catccattgg ttctaccttc
    10861 agattgtaca aaaacccacc gataaaacca gtgttatagt agttcatttt ctggctgtaa
    10921 ggtatccata gtgccgtttt attatatgct tttcgcaaca aattatcggg attgttgaaa
    10981 ttactgatat accctagcag tccaattgcc acaagcagtg tcgttaatcg gatcaattgg
    11041 aatcgccttc cttttttcag gcttcgaaac atgtaccagc aaaacaatcc tatcacggtt
    11101 cctgcggcag ctaagatcac tgtaaacagc atagtgccgg tcatatcttt cagcaatccg
    11161 atttcagtga tcatcttcaa atcgtctgga taaatcggtt cttgccgata actcattttc
    11221 atataatttg caaaacctaa tacaccgatg gctacgacat acgtaaggga acctaaaggg
    11281 acagatcctg ctagtgcaat cagaaaaata agaataatca gcaagacgag acaggctaag
    11341 aagaattttt ccgtatgcca agaaaaagca aatttcaacg caaggtctac ggatagatca
    11401 ttttgacacc actgcaaaaa taaattacta aaaacaacaa gaaaaaagat actgatgatt
    11461 gctgctgttc cttttaaaat ctttttctgt ttggtcgaaa gtctgcttat atatcttttc
    11521 aaatgcttgc tccttcattt ctatcaataa aagaatgttg cttctcttat tttagttgta
    11581 cgaaattcga aagtaaagaa cggaaagaag aattaatgaa atccaaatat tctctttctt
    11641 tatggccttt ctggtttctt taataagcgg acctttcgta ctccagaaat tttttctagc
    11701 tgttgtttca cttgattgag ctgttcaaaa tcagtactgt ctatgtcaat caatatgtag
    11761 acatgctcat tcttatggtt acgcgatata tctgcgatcg ctagctcgtt ctcattcagc
    11821 agagaaaata tctctgctaa tatattggtt ttcttttgat aaaatatagt gaatcgaaac
    11881 ggcgcttgga aaaacattcg tgcggatgga taattgaccg cttctctaac cgtaccgaac
    11941 aaaagataat cttttaaggc ttcaactgcc aaacgtccgc tgtctgccaa agcttcctga
    12001 gtcgatcccc tagatgaggc aacataagta tacggtcatt ttctaagaac tcttcttctg
    12061 gaaaatcagt caagtattta gccagatgtt gtttttccaa tgcttgcata acagcatatt
    12121 tatcaacaat ttcacttctt cctacattga taagtacagc atcttttttc attttttcta
    12181 tattcgcctg atcgatcaat ccttttgtat cttctgtcaa cggcagcatg actacgatgt
    12241 aatccgaggt agaaagaact gtttctaaat ctgcttgccg aacataatcc aactgggcat
    12301 cgcggataga ataacccaag acatccatcc ctaagctata gcaacttagc gctacttttg
    12361 tgcctatcgc tccaagacca agaagaccga tggttttgcc ttgcaattct cgtcccacat
    12421 aagcacttct tttgttttct gcctgttcta aaatattagg acctgttagt gtttgtacca
    12481 tacgacttgc ttcgattatc ggacgggaag ataagagcaa acagcacaaa acaagctctt
    12541 ttactgcatt agcatttaca cctggtgtat tcatgacgat cgtcccgttt tcagaagctt
    12601 tttcaacgtt gatcgtgttc accccaacac cagctcttga aattgctagc aaatctggtg
    12661 taagccattc atctttcact ttcgtactac gaataactat tccttcggct ggcaaatcat
    12721 ctattatttt gaaatattcc ccttgtaact tttctatctc ttgttctgga atagcatcaa
    12781 tcaaatgaac gtgatacata aaaccccctc cttactaaca agtatctcga aatttctgca
    12841 attagacaac tgtttaccat ttgacttcct agaagcaaaa agagcagtta gagcagagta
    12901 ggctgagtga taaaaataag gctatgacaa gttactgcca taagccttat tttcgagagc
    12961 aaaatattga gggtgagata tcttgataaa tggtgttccg ttagctgaca ttgataataa
    13021 gactaaattt caaaaaagtt gaaaagcaaa tttcgtcaat ctcttcttat ttcttaaaac
    13081 taagtggctt tttcacaacc tctttttatc taataaccac tgtttccaac tcaactaatt
    13141 ttgcccacgt ttcaatttga tctgttgtta gatttagcga gacaacggta tgatgaccgc
    13201 cacctgcctg aatccatgaa tggacacctt catggaaatt cggtttagtt ttccataaca
    13261 cacgtgcaac aggtaaatta ggtgcttcag tcgttggttc aaaggcttct acttcattga
    13321 tcagcagttt ataatgcgta ccgaaatctg ccatcgatac gactacccct tctcctgctt
    13381 tcccatcaaa tacaagacgc gccggatctt ctctatctcc cattgataaa ggagaaacaa
    13441 cgattcttgg tttcgtcgca gcaagcgtcg gatctacttc catcatatgg gattccaaga
    13501 ttgcttcttg gccactagct agctcgtaag tataatcttc catgaatcct gtatctttcc
    13561 ctcgagccat gattttcagc aaccgatcaa tcgctgcagt tttccagtcg ccttccccag
    13621 caaaaccgta tccttctgcc atcaaacgtt gtacagctag tccaggtagt tgtttcattc
    13681 catacaggtc ttcaaaatta gtcgtaaaag cgttatagcc tccttcttct aagaaacggc
    13741 gaataccgat ttcctgctga gcttgtactt ttacatgttc ctcccatgtc ttttcttcat
    13801 aatcaccata gtcgaaggta tacaattcct tgtactcttc aaataattga tcgacttctt
    13861 cgtctgatac gcgatcgatt acttgtacaa gatctccgat accaaaataa tcaacagtcc
    13921 aaccaaattg aatctgtgct tccactttgt ctccttctgt gacacccacg tttctcatgt
    13981 tgtcaccaaa acgagccact tttattccaa aactttcatt ataggctact gcaacatcca
    14041 tccaatcagc aatctctttt tggatctcag gtcgtttcca gtagccaacg acgattttat
    14101 tttgtttatt taatcgagca ttgatgaacc catattcgcg gtcaccatga gcggattgat
    14161 ttaaattcat gaaatccata tcgatcgttt tccaaggaat gctttcgtta tattgcgtag
    14221 ctaaatgaag caatggtttt tgtagaagct ttgtcccgcg gatccacatt tttgctggtg
    14281 agaaagtatg catccaagtg atcacacctg ctacttcttc tcgataattg acctctttca
    14341 tgatttttgt gatcgtatca gcagtaactg ctaattcttg aaggcggatt ggataaggca
    14401 attggccatt ttcattcagt tcatcgacca tttcttgtgc atgtttcttt acttctcgca
    14461 gtgcttcttc cccatataaa tgctgtgatc ctacaacaaa ccaaaattct ttttctccaa
    14521 tattcaacat aattagccac tccttttatt tgttttgtcc gtaatacgca ttttttccgt
    14581 gttttcgtaa ataatgttta tctaaaatcc gttgaggcag ctcttctgaa aatgaattga
    14641 gctgtctggt aaataaattc atcttgcata cttcatctag tacgactgcg ttcattaccg
    14701 cactttgcgc atctttcccc caagtaaatg gaccgtgtcc gtgtaaaagt acccctggaa
    14761 ttgctaaggg atcaattttg cgttctttga atgtttctat gataactttt cctgtttcat
    14821 attcgtaccc gctgtcgatt tcctgttgtg tcaaaaatcg agcacaaggt accgatccat
    14881 aaaaagtatc tgcatgcgtc gtccccatcg caggaacatc caatcctgct tgtgcccaga
    14941 tcgtcgccca tgtcgagtga gtatggacga ttcctccgat ttgaggaaat gaacgatata
    15001 aaacagcatg agtaggcgta tccgaagagg gattgagttt accttcaatg atatgattgt
    15061 ccaagtccac aacgaccatg tcgtctgctg tcatctcttc ataactgact ccgctaggtt
    15121 tgataacgaa atatcgtgtt tcaggatcaa aagcgctcac atttccccat gtatatttga
    15181 cgagtccgtg tttaggcaag tcaagatttg cttggtatac ttcttctttc agttgttcca
    15241 gcattttcta tcactccttt ttcttgcaat tgatgaatcg ctgttttttc aatgtcgatc
    15301 ccctcacggt aacgtctcat aaatatctga tagcctgcta tttcttctgc tgaaggtgtg
    15361 atggtgattc cttggtcttg tccaaacacg tcttctgcca agaagttttc taaagacttc
    15421 ccatgtcgat ccatcatata ggctgctaat aacgcgattc cccacgctcc accttctccg
    15481 gctgtgtcca tcactgtaac tggtgcctcc atagcagatg ctagaatccg ctgtccaact
    15541 tctggtgttt taaaaatccc cccatgcccc actaaacgat caatttggac attctcagat
    15601 ttcaatatat ccattccgat tctcattgca ccaaaagcac tggctaaatg gatccgcatg
    15661 aaattcgcta aatcaaaacg gctacctggt gtacgtacaa ataatggcct tccttcattt
    15721 actcctgtaa tgttttcacc ggaaaaataa ccataagaaa gtaatccccc gcaatccgta
    15781 tcgccttcta gcgctttgtt gaataatacc gtgaataatt tatctctgct tatctccata
    15841 cccaaagaat tggtaaattc ttcaaaaata ctgacccaag cattgatatc cgaagagcag
    15901 ttattcgtat gaaccatgcc tactagttct cctgaaggtg tagtgaccat gtcgatttcc
    15961 ggatggacat tttctagttc tttttctaat acgatcatag cgaaggcgga agtaccagcc
    16021 gaaatgttcc ctgtcccttt ggctacactg ttcgtagcga ccataccggt tcctgcatcc
    16081 ccttctggcg gacaaaccgg gattcctggt ctcaaatttc ccgtagggtc gatcagataa
    16141 gcccctgatt cgcttaactt gccagcttct tctcctgcta ctagaacttt aggcaaaatg
    16201 tgatccaagt caatcgctac gtgcgctttt tcgattaact gaccaaaaat atttttcatt
    16261 tcttgatggt aggttttcgt tgaaggatcg ataggaaaca tgcctgatgc atctcctacg
    16321 cctagtactt tctcaccagt aagctgccaa tggacataac ctgcaagcgt tgtcaaaaaa
    16381 ttaatttctt tcacatgttc ttctttattc aaaatagatt gatacaaatg agcgatactc
    16441 catctctgcg gaatattgta ttgaaatttt tccgttaata ttcgttctgc ttcttcagtg
    16501 attgtatttc gccaagtacg aaatggtact agtaactgtt ccttgctatc aaatgctaga
    16561 taaccgtgca tcattgcact gaacccaatc gacccgattg tcgtaagatt ctcgccatag
    16621 atttcgaata cttctgctgc catttttcgg taactgacgc gcagtccttt ccaaatatct
    16681 tctaatgaat aggtccagat accattttct agtttgtttt cccactcata actaccagcg
    16741 gcaataggag taaaagatga atcaatcaat acagctttga tcctagttga tccaaactca
    16801 atccctaaag atgtacgacc atctatgaca tctaacatac tttgcgcatt tacttccttc
    16861 ataatgattt aactccctcc ttctcgtcaa acctgatcaa accgtctttt tagtagcgct
    16921 tacaatgatt attgtatact tttgtacgta catgtcaagc agttatttac aagaatacaa
    16981 taaaaaacac ttgttttgta cgtacataag ttataaaatg aaattgctta caaaatctga
    17041 tcaaccgcct tctcgaaatt ctagaaggag agaatactta tgaaaaaaga aaaaaaagtt
    17101 tcctcatcgt ttatttattt ttttggcgca tttggcggca ttctatttgg atatgatatt
    17161 ggtgttatga ccggcgcctt acctttttta cagcatgatt ggggtttggc aggtaaagct
    17221 tctttgatag gatggatcac gtcttcggtc atgttaggtg cgatccttgg cggctcttta
    17281 tctggtcttc tttctgataa gcttggacga cgaaaaatga ttcttctttc tgcacttatt
    17341 tttatggctg gttctgtcct ttctgcatct gcccctcaca atggttctta ttttctgatt
    17401 gcagcaagga ttcttctagg tttagctgtt ggcgctgcat ctgccctagt tcctgcttat
    17461 atgtctgaaa tggctcctgc tagactaagg ggaagattgt cagggatcaa tcaagtgatg
    17521 attgcttctg gcatgctcct ttcctatgtt gcagattatt tgctaaaagg gttgcctgaa
    17581 acgatggctt ggcgcgtgat gttagggctc gctgctgtcc ccgcgctgat tttatttttc
    17641 ggtgttttag ctctaccaga gtcaccgcgt tttcttatgc aatctggccg cttggaagag
    17701 gcaaaaagag tattgaacta tattcggaca ccaaaagaag ctgaacagga attcgaacaa
    17761 atccagctga atgtcaaaca agaaaagaca accggaactt cttggcatac tttgttttta
    17821 gaaaaatatc gttccttagt cattgccggt attggtgtag cagtattcca gcaatttcaa
    17881 ggagctaatg ctatttttta ctatattccg ctgatcgtag aaaaagcgac tgggcatgca
    17941 gcgagcgatg cattgatgtg gccgatcatc caaggaatca ttttagttgc tggttctttg
    18001 ctctttttgg ttattgcaga caagttcaat cggcgaactt tattgaaaat aggcggtagc
    18061 gtgatgggac tttctttcat cctaccagct gttttaggaa ctgtactgga tgctcataca
    18121 aattcgttgt tgattctctt gtttttatgt atttatgtcg cattttattc ttgcacatgg
    18181 gctccactga catgggtaat cgtaggcgaa atattcccac tagctgtccg cgggcgagca
    18241 tctggcctag cctcttcttt taattggatt ggttctttcc tagttggctt gctttttcct
    18301 gtgatgactg cttctatgtc tcaagaaatc gttttcggca tttttgggat catctgcttt
    18361 ttaggggttt tatttattca agagatcgta ccagaaactc gtgggaaatc tttagaggaa
    18421 atcgaacaaa gtgcatctaa aaaaacttat cctaaacgta tttctatcca tcattcttaa
    18481 agaggttgtg acagaagtgg tcagcttcaa gaaataagaa ggaaccgctt agtttccagt
    18541 cacaaggaac aatttcaaaa aacagcttct catgtttgat gaatattgtg acttgtgacc
    18601 agttggtagg agaacaccgt ttagtggaga ggtggtgaaa gaaacgttca gccttgagcg
    18661 atgacaaaag ctctcacaaa cattgcgacg agtaaccgta ggagcaataa gaggcggtaa
    18721 aaacccgtct cttatttttc gttttctttc actgtttccc gttcaactag tacaggttca
    18781 tagagaatat ttggctgtgc ctgtcctttt tcaatcgttg tgacgatcca ccgtgcggca
    18841 tcttgtccca acttttcttt tggatgcgtc aatgttgtca attttttatt ccctaaatgg
    18901 cttaaagaag aatcgtctgt tcctacgatc gataggtcat ctggaatatg atagccattt
    18961 tctgtcaact tatctagcat taaactagct atttgatcgt tatagcaaat gatgccatct
    19021 aaagcttgtt cttctttcaa ccgttgtact gttttttcag cgattgtttc tcgtgtttcc
    19081 gtggtgaaag taatgatatc ttccgtcaaa aaacgcacat tatactgttc acacgcgctg
    19141 ataaaacctt tcatcctgta ttttccttga agatcgtcga tttttgtgat gaacaacaga
    19201 cgctgatgtc ctttttttat caaatattct gctgccataa aaccgacttt cacatcatct
    19261 aaacaaatat aagaggcatt taactcttca taagcagcat tgatcatcac catcggaatg
    19321 ccttgttctc tcaaagcaac ataaagtgct aagtttgggt tatactgatt acttttcgta
    19381 ggctcaacga tcaaaccatc taccccataa gcaatcattt tcttcaaaca ctctttctcc
    19441 aattcgtgat cgttattcgt actggctaaa agcaatgaat acccagattc tcgtaattca
    19501 ttttcgattc ctcggataat cgacggaaaa atataatcag agagatatgt ggtgatgaca
    19561 ccgatcgttt ttgttctgtt caatggtgtt ttttctttct tatagctgtc atctacatat
    19621 gtgcccgacc ctttttcttt acgcagatag ccctcattta ccaacacagc gattgcttgc
    19681 cgaatcgtat gtctgctgac gtcatatatt tcttgcagct tgaattctga gggaatggtt
    19741 gtatttactt cgtattcctc acgaaggatc ttttgtttta tctcttctgc aatatcttga
    19801 tattttgcca tttgccttgc ccctttttat ttgtacgtac atatatcata acgagtctgc
    19861 aaggtttatt caagcaaaaa tcaaggtttc ttgtgctcat aaggcatacg gaacaggttc
    19921 accaaattct ggaaatcctt gctcattcca cgtaaagcat tgcgcgttcg cgtgccgatt
    19981 aggattgtct aacggatccc ctttttggtt cttctctggt cgagcatgat agatcaaagt
    20041 atcaaagcac ccgtcttctg aaaccgtaaa cgagttgtga ccaggaccaa attgcttatt
    20101 tttttcagaa gaaacgaata ctggctccga taatttatgc catgattctt ttaataaaag
    20161 attcttgtgc tcatcagccc acagcaggcc catggcatag ttttcatctg ttgcactacc
    20221 agaatacgta atgaatattt tcccattgcg agtaaggact gctggacctt catttactga
    20281 aaaaccccgt ttttcccatt catactcagg tatagaaagg aggatctgct tcccctttaa
    20341 cgtccaagga ttatccattt cggatagata aagattagaa ttcccagtaa tttcaggatc
    20401 ttgttgggcc cacacataat atagctttcc ttgagaagag aaagttgttc cgtctaaact
    20461 gaaagtttcc cttttcgttt ttatttgtcc cttttctatc caatctgtat tcattggatc
    20521 tatatgctga ttttcaagta cgaacatgcg gtgatgatga gattcatcac gtatagtttt
    20581 atgattgctt gcagcaaagt aaatatacca ttttccattg atataatgga cttcaggtgc
    20641 ccagatcaat tcactcatcg gtccttcttc atgagcctgc catacaatca ggcttgcagc
    20701 atgttctaat tcattcagtg actttgccct tcttaattca atcacttggt agcctggaac
    20761 cgatcctgta aaataataat aaccatctgt atgtttatat acccaaggat ctgcccgttg
    20821 ccgaacgata ggattattat aaagtgtctt catcataaca aatttcctca cattttcttt
    20881 attctaatta cattccaaga gtatttttct atccctacat ttaattcgtc atgtttttct
    20941 attgaaaaat agtttatttc aggttttatt ttttctggtt cttcacgtgt atttttgtcc
    21001 aacagatcgc tactggttaa tgtgtagtgc tctccttgat ctagcaaatt gaaatcatgt
    21061 tcaatcgtta tcttttgttg ttcatttgtg cgattgatca agaaaatagt cagctgattg
    21121 ccgttatcta cgatcacact gtccacataa ggaacatttt ttatcttttc acaatcataa
    21181 acagggcaaa tgatttgttg tttcaatact tgacctttag catatttcga taagtgttgc
    21241 atcacataat aagttggatt cctccatgct tgtcctttag catctgtcag tattaaaggt
    21301 attgtgttta ctaacaatga ttgacaacta atctttacgc gatctgcgtg tcgtaaaata
    21361 gccagcccta atgaaccaaa taataatgta tcttccatag taaagtaaca ccaatcgatg
    21421 ggactaccga cttcaaaatc cctatgtctt ttttccggtt gggcatgtac attccattca
    21481 tcaaacgcta gatacattgt tttctcactt ctcaaatatc cttttacata atcacaagta
    21541 gcaacgattt cttcaatttg acgatcgaat cgataagctt ttgcaaggta tgtcgctgta
    21601 tcatcacgct catttttagg aggtttagtc aagtcttctt catcatactt atcaatatag
    21661 ttatgcaaag ctaaataatc tacattctca taagcttctt ctaatgcgat acgatcccat
    21721 tcagggtacg atggtaaacg tggcgtagag cttccaacta agattgtttc aattgaatca
    21781 tccactaact tcatgacttt tgaggcttca tttgctaacc taccatattc ataagcactc
    21841 ttagccccca cttgccatgg accgtctagc tcattcccta aacaccataa tttaacgcca
    21901 aaaggctttt tttgcccatt ttttattctt aaatcactcc agaaggttcc ttttggaaaa
    21961 ttacaatatt ctactaaatt ttttgcttca tcaattcctt ttgttcctaa attcaccgtg
    22021 aatatcggtt tcgcttgtac atgactgatc catttaaaaa attcgtggag tccaaaagaa
    22081 tttggttcta tacctcgcca tgctaaatca atttttttgg tcgttgttct ttcctaccta
    22141 cagtatccac ccaatcataa ccagatgtaa agtttcctcc aggatagcga atgacaccta
    22201 attctaattc ctttacataa gagaggacat cttgtctaaa cccgtcacca tcagctgttt
    22261 catggcccgg ttcataaatc cctgtataga cgacacttcc catatgctca acaaatgagc
    22321 caaacagccg agaactgatc tctcctaccg taaaatcctc tctgattttt actttcactt
    22381 cttcgtccaa cagctttccc tacctttcat ttcctatagt caagataata gatacaagtt
    22441 tccatacgcc accagcaaag ataaaaataa aaggagggaa aagaaatatg agcagtacaa
    22501 atcctaataa caatacgcaa caaagaatat ttctcaaaaa atcaaaaaat actttccatg
    22561 cactttgttc aaaaatttgt aaaaatgtga tatctttgtt ttcagaaatc acatatatcc
    22621 aggataaaag aaacatggaa taaataagta aaactacata tccactaatt gaaagtacga
    22681 ttgtctgttc ttctaataat tgattaacaa gaaaataaat aatgatcgtt aacagactaa
    22741 ggaacaagtt cttaccaaaa tgttgcttta ttccttgaaa gaaaaaataa atgatatttc
    22801 ctgatccctc acctttccat tgcgccatta cataaaaaac agcacatgta gcaggcacga
    22861 tagtaataac aggcaaacac acgattaccc agagaatacc taataagatg taatcagaaa
    22921 ccacaatcaa ctgtccataa agtttagtct caactatttt catcttcctc actctttcat
    22981 tccagacata gctgttcctt gaacaaaata tttctgtgca aaaatataaa ccactagtaa
    23041 tggcagcatt gaaataaccg ttccagccat tacaggtcca taaaaagaaa tgttctgccc
    23101 tgtaaataaa gctaaccctg ctggtaaagt tctcattttt gaactacttg tcaatatcaa
    23161 tggataaagc aagtcattcc aactattcat cagcgtgaaa atccctaatg ataaaagagt
    23221 aggtttgctc aatggcaaca tgattttcaa aaaaatatta aactcactta atccatcaat
    23281 gcgtgctgcc tcttccaaag aatctggtaa agtaataaaa aaggaacgca tcatgaagat
    23341 accaaatgca gttgtcatac gaggaatgat caaacctgca taagtatcaa gcatgccaag
    23401 caaattgact tcaataaata gaggaatcat gaagacttgg aatgggatca tcattgtcag
    23461 caaaacgaaa tagaacaaaa ctgattttcc tttgaaacgc atacgagcaa atgcataccc
    23521 cgcaagagaa tcaaagaata cagaagtgat cactgtccct ccagcaaaaa tcactgtatt
    23581 tttgatgtaa tctaataaag ggatcgttgt ccaaacctta atatagttat ctagggtata
    23641 gctatctgca aatattctag gcggaaatga gataatgtct ttttcgtgtt taaaagaaga
    23701 aataatcagc cacaaaaaag gaaacgcaat aaccaacaat ataactagca tactaatcgt
    23761 tcgaatgatt tttttaccac taactgcttt tcgactattc acagtaactg taatcgagct
    23821 ttctggtgcc atacaggttt ccctcctatt tcatttgttt ttctttccgt gacatgtaca
    23881 cattagtgga gatagctata gttgcaacaa tgataaacaa aaccgaagac agtgctgatg
    23941 catagcccaa ttcataaggt gctgtaaatc ctcgactgta aatatattga acagctgttt
    24001 ctgtcttaaa ttgtgggcca ccagcggttg caacatatac ttggtcaaac acttgcatcg
    24061 aaccaatcag attcgttaaa atacaaaaac ctaaggaggg aacgatctga ggtagcgtaa
    24121 tgttaaaaaa ctgctgtcga tttgttgcac catccatttg agcagattca tacaaagagg
    24181 gagagattcc ctgaatagca gtcaagagaa tcgtcatagt aacaccaaaa ttcttccaaa
    24241 cagtcatgat ggctaccgtc ggcatagcaa gggttggatc gcgaaaaaat tgagggatcg
    24301 gcaagccgac ttgtgaaaac aagtaaggaa tcgcccctac attcggatca agcaacatag
    24361 aaaaaataat cgcaattgac gtcaatgaac aaataactgg aatgtaaaat gtcgaacgac
    24421 aaaaacgatt gaatctggta tttttagata atgctactgc tgctaataaa ccaacgataa
    24481 tttgtgttgg cgtttcgagt acggtgaaat ataaagaatg aatcaacgaa ttaactgctc
    24541 gttcatcact aaatatacgt gtgaaatttt caaaacttac aaaatttctt gatgtaaaga
    24601 aaatattcaa atctgtaaaa ctaatagcaa atgttcctaa aagcggtatg acagtaaata
    24661 ataataaaac taaaaaagat ggagttaaaa acaaatacgc agcacgatca ggacgattcc
    24721 aatatttggt gaatccgttc atatagtcca cctcatcttt cattttgata aaatatacaa
    24781 tcttggttta aacgaatctg aataagatat ccgaacaatc gctgtgattt ttgcaataac
    24841 acctatctgc aaatactagg tcgatcattc agatatctgt atcttgccaa cagtctatcc
    24901 tgtataaagt tctgtactat ttttgcagta attgattgat ggcatcatct gcatcgttca
    24961 tgagtgtctc aggatcttct cctgctaata atttttcaat cagtggattg atgatatcgt
    25021 tattgatcgc agagatttgg gtaaaacctg gtaaatatgg ctctgcatag tccatttgtt
    25081 tcgataattc agatacaata ggatcatttt ttacttcttc atcttttgct actgaatcca
    25141 agtaggcagg aaaaccattt tcgatactcc attttttacc aatttcagtt gtattccaat
    25201 atttcaaaaa ctcatagaca gcttcttttt tattattatc tgtacttgcc ggaatggcat
    25261 atcctacgcc atctaaaata gctgtttttt tgtctgaatc actcccttgt ggtaaggtag
    25321 tcacattata atcaatctcg tttgctttca atccgttatt taaccatggc ccattgattt
    25381 caatggctag ttgaccagca ttcattaaat tgtccatttc ggcgccagaa atcgattctg
    25441 gaccagtctt gtctacatgg atcatttttt ggatcgcttt taacgtttta aggttctcag
    25501 gagacgcaaa ttcagactta gtataatcat cgttcactaa tttaccgcca tttgctaaca
    25561 tccagttata taacggtgca gttccatcct tattgaagac aaaacctgat acatttttac
    25621 tactatcagt taattttggt gccatttctg tcaattcttc ccaagtagta ggtggttgtt
    25681 cagtatctaa tccagcttgt ttaaacaaat cattattcca atacatatac atgccttgta
    25741 cctgcatggg gacaaaatac tgtttacctt ctacttcacc taattcaata gccgtttctt
    25801 taaaatcaga tttatctact ccatcatatt cccagaaatc gtctaatggt tgtaccgctc
    25861 cgttttctac atactgtgca aaatctggat aatttaatgc aataaagtca ggtgcttttt
    25921 tagaagaaat cgctgtagga agtttttcat tcaaattggc ccaagtcatg atatccattt
    25981 caattttgat gtttttatca tttgttttat taaattcatt aacgatttct tgcaatattt
    26041 caccatcaga tgcagtaaac ccattccaaa aagtcaaagt cacttcttta tcagaatcag
    26101 attctgctga acttccgcca ccgcaagctc ctaaaatacc tgttgcagct accaacaaag
    26161 agccaattaa ccatttttta cctttcattt tttgttcctc cttcatctga taaacgacat
    26221 ctgaccacat tccaagatag cggtttcaat aaacaattga ttttcccatc ttcagctttt
    26281 acatttgttg aatgacttgg ttttacttgt tcacttttag gaagattagt tgcttttgta
    26341 tcaaatccac tcatttcaga aaaatcaatc actccctcta atgataattc agacaagtga
    26401 acagttagat tcatttcttc ttctccacgg ttaactgcaa atatgacaag ttctttgtca
    26461 tttattacgg ctatactttc taaatacggc acttcttcaa aatcttccgt cgcatagttt
    26521 gctgaacgaa tttgtgggct taacacctct ccttgtccat acacagcagc ttgcataaat
    26581 ggataataga tcgtctgtct ccaagcttct ccgtttgttt ctgtcctgat tggtgcaata
    26641 acatttacca attgagcaag acaagcaatc tttactctat ctgcatgttt caataaagtg
    26701 attaatagac agcctaatag taatgcatct tcaaaattat agttatcttc tagtaaagga
    26761 ggagcaatct gccaaggcgc tgcttgctca tcttgttccg ctgaatgata ccaaatattc
    26821 cattcatcaa aggacaggtt gatctgcttc ttactatgtt tcttcgcctt gactgtatcg
    26881 cacatcgctg tcacttcaga aatgaaccga tccatgtcta aggaacgtgc aagataatga
    26941 gaaagatcat ttttttggtt gccataatat tgatgcagag acaaataatc tatttgatcg
    27001 tacgcttgat caagaactgt taactcccaa tcaccaaatg taggcatttg ccgattcgaa
    27061 cttccgcata aaaccacctc aattgaatca tctacgagtt tcatcgcctt agccgtttcc
    27121 tctgctaacc gtccatattc ttccgctgtt ttatgtccaa tctgccaagg accgtccatt
    27181 tcattcccta aacaccatgt cttaattcca aaagggctct cttgaccatt cgaacgcctt
    27241 aaatcactcc aataagttcc tttaggaaaa ttacaatatt cgactaaatt acgagctgca
    27301 tctactccac gagtacccag attgacagcc atattgattt cggcccccac atgctgacac
    27361 cagttataaa attcatgaat cccaacttca ttcgtttcaa ttgtccgcca agctaaatcc
    27421 aaccttctag gtctatttgc tatgggccca atactgtctt cccaacgaaa tcctgacaca
    27481 aaatttccac caggatatct aatcatggga atgttcaatt cttttaccaa gtgaatgacg
    27541 tcttggcgaa atccactttt attagctgat ggatgatctg gctgataaat cccttcatat
    27601 actgctcgtc ccatatgttc aataaatgaa ctgtacaacc gcggatcaac tttgcctaca
    27661 cgcttgacac tactaatcaa cattgtggct tccataactg acctccttta atttatacat
    27721 gaataataca cctcgttgaa agcgttgtca ataatataaa tgttaaactt acttattttt
    27781 tatcgatttt aattaaccaa aatattaata atgattaaaa caaataattt aatataactt
    27841 ttattaacaa atatgttaat atataagaaa aaccttttaa gaggtgatat atatgcaact
    27901 acaaataaac gaagaatcct taccggtata tgaagcatta gctagtaaaa caaggatcaa
    27961 gatcatccag ctactttcca aaaaaaagat gaatgtcaaa gatttggcaa aagaacttgg
    28021 agtgagcagt gctatcacga cgatgcacgt gaaaaagcta gaggaagcaa acatcatcaa
    28081 aacagaaaaa gtaggacaac aaaaaatttc gagccttcgt gtcgataaaa tcgatatcag
    28141 ttttccagaa aaaatattca atgcttttga tacgaaagaa acatcgatac ctattggtca
    28201 ctatacaaat tatgcaattg aaccaacatg cggtttagct acgatccatg attttatcgg
    28261 gaaagtggat gaaccacgct attttatgga tcctcgaagg atggatgccc gcattctctg
    28321 gtttaccagc ggatttgtcg agtatcaggc acctaatttt ttaaatacag aggatacatt
    28381 ggagatgttg gaagtttctg tggaaatttc ttctgaattt cctttttcaa atgacaattg
    28441 gccttcagat atcactttca gcctaaatgg tgtggagcta ggaacatgga cgagccctgg
    28501 agattttgct gatatccgcg gtaaatatac gcctgattgg tatccagata atcttaatca
    28561 atacggcttg ctgaagacga tccgaattac caaacacttg acgaatatga atggagaacc
    28621 cttgtcaaac atcacgatca acgacatccc taaagagcaa gacacttggc atttgcgaat
    28681 cgaagtgaaa gacgatgcca aacatgtggg gggctgtact ttatttggta aaggattcgg
    28741 taattacgat caggatatca aattaaaagt ttactacagc tgatattttt cttttctatt
    28801 ttataaaaca aaaacagcca atccctatga agggacggct gtttttgctt tattgttatt
    28861 tccagaaatc atccaaaacg gaaattggca aatgtcgttt atgttgtgtc ttgttccacc
    28921 atgcttcgat tttttcttgt gcttgagcgg gaacttcttt tccttctaga taatcatcaa
    28981 tttcattata tgtgacacct agtgcgactt catctgcaat cattggtttc ccatcttcaa
    29041 gatctgcagt cggaattttt gtgtataatt tttccggtgc attcaatgct tgaagcaatt
    29101 gtttcccttg tcgtttgtcc aaacggaata atggcaaaat gtcggctcca ccatcaccaa
    29161 atttcgtgaa gaatccagta atgttttctg cagcatgatc tgtccctaat acggctcctg
    29221 ctctttctcc agctacggca tattgcgtaa tcatacgttg acgtgctttg atatttcctt
    29281 tgttaaaatc gctgattccc acacctgctt cgcttaatac ttgtacttgt gcatcaacag
    29341 ctggtttgat atttactcgc agactaacat cgggctggat aaagtcaaga gcagcttttg
    29401 catcttcttc atctgcttgt tcaccataag gaagacgaac agcgataaat tgatagtttt
    29461 catcttttgt ttcttcacgc atttcttcca tcgttagttg cgccaaacgg cctgctaaag
    29521 tagaatcttg gccgccgctg atacctagta caaaagtttt aagaaacgga tattttttca
    29581 aatatgcttt cataaagtca atacttttac gtatttcttc ttttggatca attgttggtt
    29641 tgacacctaa ttcattgata atttctgtct gcaggctcat ttattctcct cctatgcttc
    29701 tgtatgaagt tctgctacgc tttttttcat tcgttccatg atagccattt tatggttcca
    29761 gcattcagta gacagatcga ctgggtattt ttgcggattc aagtctcgtt tatactcttc
    29821 ccatagtgaa tctagatttt cttttgtata ttctttgatt tcgtttagtg caggtaattc
    29881 gtaaactctt tttccttcga taaagatatc ttgtaaaact gggcgagctg taaagtctcg
    29941 aacagtttta ttgataaacg tatgcactgg atggaacatg aagatagctt cttcttctcg
    30001 agggtcttca tcccataatg tcacgtagtc tccttcagat ttcccatcaa aatttcgagt
    30061 gatgcgccac acttgttttt tccctggagt agttactttt tctgcattgc ttgataactt
    30121 gatcgtatcc atcatttttc cttcatcatc ttcgatcgat actagcttga atactgcacc
    30181 aagggctggc tgatcatagg ctgtgatcaa ttttgtccca acaccccata catcaatttt
    30241 agctttttgc atctttaaat tcaaaatcgt tgcttcatcc aagtcattcg aagcatagat
    30301 ttttgcttcc gtaaatccgg ctgcatctaa ttgttcccga acacgcttag atatataggc
    30361 catatctcca ctgtcgatgc gtacgccttg gaagttgatt ttgtcaccta attctttcgc
    30421 cacgcgaatc gcacttggca cacctgattt caacgtatca taagtgtcga ctaagaacac
    30481 gcagtcttta tgggttttag cgtaggccat aaatgcttca taatcatttc cataagattg
    30541 gactaacgaa tgagcatggg tcccgctagc tggtatccca aatatcttcc cagcacgtac
    30601 attgcttgtt gcatctgctc cgccgatata tgccgctcta gttccccaga ctgctgcatc
    30661 cagttcttgt gcccgtctag ttccaaattc caatagtgga tcttctccga tgacagattt
    30721 gatacgagct gcttttgtcg cgatcaacgt ttggaaattg accatattca acagtgctgt
    30781 ttctactaat tggcagtgcg ctaaaggccc ttccacttgg attaatggtt catttgcaaa
    30841 aactagttct ccttcacgag cagaacgaac tgttgcttta aattcaaaat tttgtaaata
    30901 ctccaaaaaa ccttcaggat agacttctaa tccacgcaag taagcaatat cggaatcatt
    30961 gaacgtcaga ttctctaaat aattgactaa ccgttccaat cctgcaaaaa ccgcgtatcc
    31021 gtggttaaaa ggcatttctc gaaagtaaca ttcaaaaaca gcatgtaaat cagctcgacc
    31081 taattcccag tatgtctgca tcatattgat ttgatataga tccgtatgca gcgtcaaact
    31141 gtcgtctggg taagtattat tcatcttgta ttactcctct tggcaagttt tcatttccta
    31201 cccattataa caaaaaaaat taataggaaa agaaatcttc cattttttct gaaaaagcac
    31261 atgttttacg gatatttctt gctaaaaatg aactccatct gctaaaacta tcctttctta
    31321 ttggtattta acctaaacga gaaagaatac ttattttatt caatgtatcc gagacacgaa
    31381 gaaattagcg ttttctattt ttcttacttg ataaaataag tagattttcc tactacaatc
    31441 gtagccgtta agataccgat gattgaacaa atataaccaa atgtctctac aacataatca
    31501 atggcaaaag tttttgaagc aataatacgt ctttcctttt ttgcttctta tccccaaaga
    31561 aacggatata acttattatg tattgaattt ttttggaaag gcgaattatg acaagatata
    31621 ccttgcgttg aaaaaactcg cggaaaaaat atcacacgtt tttaaaaaga tacaaagtaa
    31681 gaaagcagct gtcaatagaa tggaaacaaa aatggcgaga catcaatcag tctcgccttg
    31741 ttttttattg tatacattag atatggagca ggttaccata aagatgatta ttagtcgtta
    31801 ttaaatagaa tccttcgtat ttttttgttc tatcactaca atatctttat ttggacgttt
    31861 gaattgtatt ccggcacgtt ctagctcctg tttcactcct acatggactt tgacatcttc
    31921 tcgtgtcatt tttaaaacgt gatgaatcgc atagtcgatt tcttgccttg tgattttttt
    31981 cttcgtattg atcatcacga cttgcttttt atcatatgtg tctaccaata gcagttgtgt
    32041 tcgatctaac gaaaataatt tgtaataatt aatagccgta ttcccaaaaa agaattccac
    32101 tactttagga taaaatgtct taatatttaa attactattc acaggtgttt cgattagctt
    32161 catctcattc ctcttttcta tatagtcgat ttagatccaa tcttaagtta tgttaccatc
    32221 tttcctagca ttaaggaatc ggttacaatt gaacctttaa agacaaaagg atttaccttt
    32281 tatcacttaa tagtataaca taatttcgtg agaaaaaaat acaatttttt cttgaccatc
    32341 tcgttacgta aaggattatg cttacatttg tcaggaggaa aaataatgga gtacacaata
    32401 aagaaaatgt cagagatatc aggagtcagt ccgagaacct tgcgctttta tgatgagatt
    32461 ggactgctaa aaccagctag gatcaattct tcaggttatc ggatttatgg caaaaaagaa
    32521 gtcgatcgtc tgcaacacat cttattttat cgaacaatgg cattcaagtt ggatgatatc
    32581 caagaggtct tagacaatcc atcttttgac caccagaaag ctttaatcaa gcatcagcaa
    32641 atgttgctgg aaaaacgggc acaaatcgat accctcttga caactgtcca acagacactt
    32701 gatatgtacg aaggaggaag aaaaatgagt gacaaagaaa aatttgaagg gtttaaacaa
    32761 caaaagttga aagaaaatga agaatcctat ggacaagaga ttcgacaaaa atacggaaaa
    32821 aaaaaggtta cagcatccaa tgaaaaatgg atgaatatgg ataaagaaac gtatgaagaa
    32881 atgcaaactg ttgaaaagca gatgttattt gatctcactc tttacttaaa agaaccagaa
    32941 gatgaaagct tagcagaccg tatcttccaa gctcataaga agtggttctc ttacagctgg
    33001 cctgaatatc agccaaaagc gcataaagga ctaggaatga tgtacgaagc cgatgaacgg
    33061 ttcactgcat attatgacga gcgaagtggt catggtgcag caaaagcttt aaatgaaatt
    33121 atccaaaaaa aggcgtaatc ttttacatga tccaacaaaa aagtacacgt aaaatgacct
    33181 aataacaaaa cagcagacac ccaagttaga tttttatgct aacttttggt gtctgctcca
    33241 ttctttcaca agttttttca tcctacttta aatatattca tcgttgaata aacgtataag
    33301 gtattttcac tttatccttt ttttctgtaa gaaacaattg aaatgccatt tctccgcaag
    33361 cttccagatg gtggtcgatg gttgggaaac caagaagctc gctgatcagc aaattatcgc
    33421 gtccaatcac cactggtgtc ttatcttgca gatagctttg caagatgcct gctgcaacca
    33481 tatccgaatt tgtaaagaca ccatcaactc caagctcttt aaaaaagaag cctgcttgtt
    33541 ttcctttctc tgcatctata caatcccaaa aaacaagtga ttcatcaaaa gatggaaaaa
    33601 gttgatgaca tagctgcaaa gtgatttttg agttataact tagcctgcca cttcgcccca
    33661 acgtgatgcc caattgcttt ataccttgct gcttcaaata agtaagacct tctgtaattg
    33721 attgttctcg atcaataaag acacagcttg cctgttttcc cttgatttct tcacaaaaaa
    33781 ctagaggtcc atatttttga tatgccaata aatcatctag tgtattggca cgagacgtaa
    33841 tgatcaatcc atcgaaagct ttagctgcaa attcttcaag ataccttcgc tctttttctt
    33901 tatgatagcc cgtcggtaaa agtgtcactt tgtaatcata atcaaacgct gcttcaatga
    33961 tcccactgat caactgatca aaatacggat gattggtata aggcagaatc actccaatgc
    34021 ttttcgtttt tccataactt aactgtctgg catttgcatt agggatataa tcgacttctt
    34081 tcatgaccgc aagtatttct ttcctttttt cctcatctac atatggatgt ccattgatta
    34141 cacgtgaaat agtagaaact gaataacctg tgagctttgc gatatctcga atgtttgcca
    34201 ctgctgcttc gccacctata caaaaagatt ttcttgtaaa tagtctacca cagctggacc
    34261 gcattcaaaa aactgataca tttttccttc atgcgaaaaa ctgatccgct gcccgcccca
    34321 atatatatct tttgtcaaat tttcgatttc attcattggt atttctttta ctatattcct
    34381 attcaaccct tgaaaactgt acaacgttaa tacggatccc ttgaactcga caaacgcata
    34441 aaaataattg ttcgttgtgc catctgttat ctctacataa gtattcattg caagccctcc
    34501 ttttgatact ttgagtatag tgtatgaaac gcgtctcata gcaaggagtt tgttttgcca
    34561 aagaaaagtt ttattcctct ttcattatta ccaaagtctc ttattttaaa ttgaatacat
    34621 tataatgata ctgtaaatat atctttatta ttagtgaatg
//
LOCUS       DACKAC010000024        33888 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.024, whole genome shotgun sequence.
ACCESSION   DACKAC010000024 DACKAC010000000
VERSION     DACKAC010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33888
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.024"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            370..2031
                     /locus_tag="I0B46_001215"
     CDS             370..2031
                     /locus_tag="I0B46_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAR2083527.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2101..2448)
                     /locus_tag="I0B46_001216"
     CDS             complement(2101..2448)
                     /locus_tag="I0B46_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAR2083528.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2532..3473
                     /locus_tag="I0B46_001217"
     CDS             2532..3473
                     /locus_tag="I0B46_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAR2083529.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3474..4517
                     /locus_tag="I0B46_001218"
     CDS             3474..4517
                     /locus_tag="I0B46_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAR2083530.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4523..5593
                     /locus_tag="I0B46_001219"
     CDS             4523..5593
                     /locus_tag="I0B46_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAR2083531.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5583..6536
                     /locus_tag="I0B46_001220"
     CDS             5583..6536
                     /locus_tag="I0B46_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAR2083532.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6548..7558
                     /locus_tag="I0B46_001221"
     CDS             6548..7558
                     /locus_tag="I0B46_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAR2083533.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7843..8664)
                     /locus_tag="I0B46_001222"
     CDS             complement(7843..8664)
                     /locus_tag="I0B46_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAR2083534.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8761..9039)
                     /locus_tag="I0B46_001223"
     CDS             complement(8761..9039)
                     /locus_tag="I0B46_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083535.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9307..11316
                     /gene="metG"
                     /locus_tag="I0B46_001224"
     CDS             9307..11316
                     /gene="metG"
                     /locus_tag="I0B46_001224"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAR2083536.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11795..12382)
                     /locus_tag="I0B46_001225"
     CDS             complement(11795..12382)
                     /locus_tag="I0B46_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083537.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12557..13879
                     /locus_tag="I0B46_001226"
     CDS             12557..13879
                     /locus_tag="I0B46_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAR2083538.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            14034..14810
                     /locus_tag="I0B46_001227"
     CDS             14034..14810
                     /locus_tag="I0B46_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAR2083539.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14803..15372
                     /gene="rnmV"
                     /locus_tag="I0B46_001228"
     CDS             14803..15372
                     /gene="rnmV"
                     /locus_tag="I0B46_001228"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAR2083540.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15365..16249
                     /gene="rsmA"
                     /locus_tag="I0B46_001229"
     CDS             15365..16249
                     /gene="rsmA"
                     /locus_tag="I0B46_001229"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAR2083541.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16700..17329
                     /locus_tag="I0B46_001230"
     CDS             16700..17329
                     /locus_tag="I0B46_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083542.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17453..18562)
                     /gene="ugpC"
                     /locus_tag="I0B46_001231"
     CDS             complement(17453..18562)
                     /gene="ugpC"
                     /locus_tag="I0B46_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAR2083543.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18771..19193
                     /gene="mgsA"
                     /locus_tag="I0B46_001232"
     CDS             18771..19193
                     /gene="mgsA"
                     /locus_tag="I0B46_001232"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAR2083544.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            19516..20052
                     /locus_tag="I0B46_001233"
     CDS             19516..20052
                     /locus_tag="I0B46_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAR2083545.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(20092..21855)
                     /locus_tag="I0B46_001234"
     CDS             complement(20092..21855)
                     /locus_tag="I0B46_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAR2083546.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21858..23573)
                     /locus_tag="I0B46_001235"
     CDS             complement(21858..23573)
                     /locus_tag="I0B46_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAR2083547.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(24352..24576)
                     /locus_tag="I0B46_001236"
     CDS             complement(24352..24576)
                     /locus_tag="I0B46_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083548.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            24776..27544
                     /locus_tag="I0B46_001237"
     CDS             24776..27544
                     /locus_tag="I0B46_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAR2083549.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            27625..28044
                     /locus_tag="I0B46_001238"
     CDS             27625..28044
                     /locus_tag="I0B46_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAR2083550.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            28056..28526
                     /locus_tag="I0B46_001239"
     CDS             28056..28526
                     /locus_tag="I0B46_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAR2083551.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            28543..29322
                     /locus_tag="I0B46_001240"
     CDS             28543..29322
                     /locus_tag="I0B46_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAR2083552.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            29309..30130
                     /locus_tag="I0B46_001241"
     CDS             29309..30130
                     /locus_tag="I0B46_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAR2083553.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            30147..31196
                     /locus_tag="I0B46_001242"
     CDS             30147..31196
                     /locus_tag="I0B46_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAR2083554.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            31209..32213
                     /locus_tag="I0B46_001243"
     CDS             31209..32213
                     /locus_tag="I0B46_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAR2083555.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            32846..33865
                     /locus_tag="I0B46_001244"
     CDS             32846..33865
                     /locus_tag="I0B46_001244"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAR2083556.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT        11063 a         6003 c         6920 g         9902 t
ORIGIN      
        1 gaaaatggct ttctacattt ttgcataaat ttggcttaat accgagaaac tggcttttga
       61 acaccgttta ttcagataag ggactgtgac aagacttttg tcacagtttc tttttgttta
      121 ttctttttta tctttgtatg gcaataatag acaatataac gaaaattatt taatggcttt
      181 tggaagacca tggaaaacct tgtacaaagc tattttttct gaaaaattcc ttgaaacctc
      241 acgaaaaaat cattatttta tgaaaaagac aaaaaaaagt gttgattttt taataattat
      301 tcattaaact cttaaacaac ttcagaaaat attaattatt ctgaaaattc caataaaggg
      361 gtacgtaaaa tgaaaaagaa atttacattt ggtttggttg cggtatgcgg ccttgtactt
      421 gctggatgtt atggcggcag ttcagccaca agcagtagtt cagcaaaaag cggttcagca
      481 gatggaggag gcgtatttaa cttagtcgtg cctcaagaaa tgcctacagc tgacttatca
      541 gttgcaacag atacaatcag ttttacagca ttgaataacg tttatgaagg aatttaccgt
      601 cttgatgaag acagcaaacc acagccggca ggagctagcg agttggcaga agtcagtgaa
      661 gacggattaa cttacaaaat caaattacga gaagacgcaa aatggtcaaa tggtgatcct
      721 gttacagcag cggattacgt ttatggctgg caacgaacag tagacccagc tacagcttct
      781 gaatatgctt acctgtatgc gccagttgaa aatgcagaag acattacagc tggaaagaaa
      841 gataaatcag aactagggat caaagcagtt ggtgactatg aattagaaat caaattgacg
      901 aaacaaacac catatttcca atatctgctt gctttcccat catttttccc acaaaaacaa
      961 tccgtagtgg aagaaaatgg ggatgcctat gcatctgcaa gtgataaagc tgtttataat
     1021 ggaccattca cattagaaga ttttgatgga ccaggaacag atacagaatg gacttataag
     1081 aaaaatgatc attactggga caaagataca gtaaaacttt ctgaaatcaa agtcagtgta
     1141 gtaaaagaat cttctactgc attgaactta ttcaaagatg gccaagcaga tgacgtgatc
     1201 ttgtctggtg agttagcaca gcaaaacgca aacgatccag cttatacttc tgttaaagaa
     1261 gcgcggacaa gctatatcga attcaatcaa cgtgaaaaga attcaccatt caacaatgtg
     1321 aacctaagaa aagctatttc ttattctatc gatcgtgaag ctttagtaaa acaagtacta
     1381 ggtgacggat cagttgtctc tacaggattg atcccatcag atatggtaaa aagtccaaca
     1441 acgaacgaag actttgcaaa agaagctgga aaattagttt cttatgacaa agataaagca
     1501 aaagaatatt gggaaaaagc gaaaaaagaa ttgggcgttg attctttaga atttgatttg
     1561 atggcttctg atgatgattc atcgaaaaaa gtaatcgaat atgttcaaaa ctcaatccaa
     1621 gaaaacttgg atggcgtaac agtcaaacca acacctgttc cattctctgt acgtcttgac
     1681 cgctcaactt cgggtgattt tgacgcagta ctaggcggat gggctgctga ctatgcagac
     1741 ccaagcagct ttactgattt gtttgtcaca ggtaattcat acaaccgtgg tcgctggagc
     1801 aatgcagaat atgacgaagc agttaaagca gcttcatcaa cagacgctgg cgatgaacaa
     1861 gcccgttggg atgatttcca aaaagcaatc aagatcatct ctgatgatat gggtgtcgtt
     1921 ccggtgtacc aaaaagcaga aggtcaccta gtgaatgaaa aaatcaaagg tatcgttcac
     1981 catgcggcag gcgcttcatg ggattacaaa tggacatacg ttgaagaata atcgttaata
     2041 ccgtgagcat gagaaaaaac gaggctgcgg caaatgtcgc aacctcgttc tcatattcac
     2101 ttatcctaac aaagaaaaaa gcatgaaaat agcagaaaga gcaattagta ttccggcaat
     2161 gatcagccaa aagctgtaca taccgtagct ggcagacgag agcgaggaaa attctggttt
     2221 tttctttcgg ttccttgttc gtgcaagatg gaccgatcgc tgaaaatgat cgaagaaacc
     2281 cgaagaaaag acccataaaa aaccgccgat aatcagaaac ggcaatgacc ataagaatag
     2341 atcattagat aattgaatga gtgtaaagga atggttgatg agatttttta ttaaaataac
     2401 cagaatcagt agaaaagcta ctgcgtaggg gatagatttt tttttcattt gattgctcct
     2461 ttatttttgg ttcattatct aataatgtag cgaaacatct gtcagaagtc aaaagctagg
     2521 gggaaacatt agtgaatagt tatatcaaat atgtattaaa acgtgtcttt ttcatgatca
     2581 tcacgctttg gttaattgca acaatcacct ttttcttgat gcaattgctg ccgggcacgc
     2641 catatacgaa tcaggaacgt ttgagtcctg aaacaatcga gatgctaaat aaacaagtcg
     2701 gtttggataa accagttatc gtacaatatg ggatttatct ttctaatttg cttcaaggag
     2761 attttggaat ttccttccaa tttaaaaacc agccagtcgc acatttgtta gctggaagaa
     2821 tcggaccatc cttgcagtta gggttacagg caattatctt tggaacagtc ctagggacga
     2881 ttttaggaac gatctctgcc atgaagcaaa atacatgggc agatacatct tctacactcg
     2941 tagcgatatt aggtcgttcc attccaaact ttgtatttgc tgttttatta caatatatct
     3001 ttgcaatcaa attacaagtt ttgccaatcg caaaatggga tggctttgtg tacacaatct
     3061 taccaacaat tgcgttggcc atgtcgccgc ttgctgactc tgctcggttt atccgtacgg
     3121 aaatggtgga agtactgcat agtgattatg tggaattagc aaaagcaaaa ggattgagcc
     3181 gttgggaaat cgcctttaaa catggtcttc gaaatagttt gattccattg atgactttat
     3241 taggaccgtt agctgtagca ttgatgaccg gatcactggt ggtagaaaat atctttgcta
     3301 ttcctggtat cggggaacaa ttcgttaagt caatcacaac aaatgattat cctacgatca
     3361 tggctgtaac gatcctttac tcatttatgc tgatttttgt tattttgatc gtggatctat
     3421 tgtacggtct agttgatcca agaattcgtg tttcagaagg gagccgagga taaatggaaa
     3481 tgataccaaa gcgttatgaa acagttgcag atatccctgc ggatgaattt cttccattac
     3541 aaaaaaatac cgaagaagaa cgtgaacaga ttactgcacc ttctttgaat tttatccaag
     3601 attcatggcg ccgtttaaag aaaaacaaag ctgccgttat ttcaatggtt ttactattgg
     3661 tcatcatttt gatctctgtg atcacaatct ttgtttctcc tcatgatcca actgcccaaa
     3721 acgtggctta tatcaatttg cctcctcgta ttccgggaat caatatcgat ggattgaatg
     3781 gaaaagcaat ggttggtggt gaacttgttg ataaatatgc tcaagcaaac gtcccttcaa
     3841 acgtaaattt ctttttagga acagacggtt tgggacgtga tgtattaagc cgcttattta
     3901 tgggaatgag aatctcgttg ctgatcgcat ttattgctgc attactagat gtaacgattg
     3961 gtgtagctta cgggttggtt tctggtttgt taggcggacg tgtcgataac gtgatgcaac
     4021 gttttttgga aatcttatca ggaattccaa acctcgttgt catgattttg atgttggtcg
     4081 tttttgaacc aggaattttt tcaatcgtgg ctgctatggc catcacaaac tggataccaa
     4141 tggcacgtat cgttcgtgca caaacaatga aattaaaaga tcaagaatat gttttagctg
     4201 gattgacatt aggtgaatca agaatgaaaa ttgcattcaa acatattttg ccgaatatct
     4261 ctagtgtcat tattattcaa atgatgttta gtattccatc tgctattttc ttcgaagcct
     4321 ttttaagctt tatcggttta ggtcttacgc ctccgtcagc ttcattagga acgatgttga
     4381 gcgaagggta caagacattc ctgtatctgc cttatctatt atggattccg gcagtaactt
     4441 tatcggttat catgattggc tttaacttac ttgctgatgg attgcgtgat gcctttgatc
     4501 ctaaaatgaa agagtgaaga cgatgacgaa aattttagaa gtgaaagact tagaaatctc
     4561 ttttgatacg tatgctggaa aggtacgagc gattcgcggt gtcaattttc atttgaataa
     4621 aggagaaaca ttggcgatcg ttggggaatc tggaagtgga aaatcagtga cgaccagaag
     4681 catcatgcgc ttgctgtcaa gcaacgccaa tatagatgct ggtcaaatcc tctttaaagg
     4741 taacgatatc gttggaaaat cagaaagaga gatgcaaaaa atccgtggta aagaaatagc
     4801 gatgattttc caagatccaa tgacctcttt agatccgacg atgcccattg ggaagcaagt
     4861 cgctgagtcc cttagaaagc ataataaagt ttcaaaaaaa gagggacaaa aagctgcatt
     4921 agaattgctt aaattagttg gaatacccga agctgagaaa cgtatcaaca gttatccgca
     4981 tcagttctca ggaggtcagc gtcaacggat cgtgatcgcg attgcactgg tatgttatcc
     5041 ggaaatcctg attgctgatg aacctacaac agcgcttgat gtgactattc aagcacagat
     5101 tttggagctg ctgaaagaaa ttcagacaaa aatcgataca tcaattatct ttatcactca
     5161 tgaccttggt gtagtagcga atgtcgctga tcgtgtcgct gtcatgtatg gaggaagaat
     5221 cgttgaagtt ggaacatcgg aagaaatttt ctacaacccg caacatccct atacttgggg
     5281 actgcttggt tcaatgccga cattagatag tgcgaacgat cgattgtatg ctattcctgg
     5341 ttctccaccg gatttattga acccgccaaa aggagacgca ttctatccaa gaaatgaatt
     5401 cgcgatgaaa atcgatgcag aacaagaacc accatttttt gaactatcaa aaacacacaa
     5461 ggcagcaact tggctacttg ctccgcaggc acccaaagtg acaccgccag aagaaatcca
     5521 acgtcgctgg gctatcttta aagagaaaca aaaacatact tacggtcaag gaggaattgc
     5581 tgatgccaaa taaactactt gaagttaaag gattgaaaca gtatttcaat gtaggaaaaa
     5641 agaatgaagt acatgctgta aatgacatca gctttcacat ttatgaagga gaaacattcg
     5701 gtttagttgg agaatctgga agcggaaaat ccactactgg ccgtacgatc attcgcttga
     5761 atgaacctac tgatggtgag atattattcg acggtcaaga tataacgaag atcaaagata
     5821 aaaaaggttt gacgaaattc cggcatgatg ttcagatgat ctttcaagat ccatacgctt
     5881 cgttgaatcc tcgtatgaaa gtcagagata tcattgccga aggaattgat gttaatggac
     5941 tagctaaatc atctgaagag cgtgcaaaaa aagtcgatga tctattgaga actgttggat
     6001 tgaatccaag ccacggtact cgttatcctc atgaattttc aggtgggcaa cgccagcgta
     6061 ttggtattgc acgagcattg gcagtaaaac cgcgttttat catttgtgat gaaccaattt
     6121 ctgcactaga tgtgtcgatc caagcccaag tggtcaattt gttgcaagat ctccaaaaag
     6181 aacatcaatt gacctatcta tttattgcac atgatttgtc gatggttaaa catattagtg
     6241 accgaatcgg tgtaatgcat aacggcttgt tattagagat gggaacaagt gatgaaatct
     6301 ataatcacgg tgttcatcct tatacagaaa gtttgttatc tgctattcct ctaccagatc
     6361 cagaccatga gagaaaacgc agaagaatca aatatcaacc tgaaccagat gacggacaag
     6421 tacgtcaact tcgtgaaatc gcaccagaac attttgtcta tgcaactgag caggaagtcc
     6481 catattacgc aaaaaaacta aaacgacaaa aggaatctct tctggttgct aactaggagg
     6541 gacatagatg ctaaaagctt ttaaattccg tatgtatccg acagaagaac aaaaacagca
     6601 attgattcgt acttttggat gtgctcgatt tacctataac catttgctga aaaagcggca
     6661 gaaaagttgg caacaaacag gagtagccaa tttttcgctc acacccgcta cattaaaaaa
     6721 agaataccca ttcttaaaag aagtagacag tcttgcttta gcaaatgcac agcttaattt
     6781 ggatcgggct tttcgcaatt atttcaaggg acgtgccagc ttcccaaagc ttaaaacgaa
     6841 aaaaagtatg tggcaatctt atacgaccaa caaccaaacc cgtacgatct atctggaaaa
     6901 tggctactta aagttaccaa aacaaaaaga actaatcaag ataaatcagc atcggccagt
     6961 tgaaggatcg attcgctccg ctacgatttc tgctcgatat aatgaagaat tttatgtcgc
     7021 actactttgt gatgtttcgc cagtaaaaaa agaatcttta gctaaatgga tcgggatcgc
     7081 ctatcatccg aagacattga tccaaacatc tcgacctcta gaagtaactt taccaaaatt
     7141 tcaccagaca gaagaaaagc tgcaacatgc acagcggaaa ttgaatgtga aagtccgctc
     7201 cgctcaccat agaaagatca ggttagatca ggcaagcaat tatcagaaac aaaagagaaa
     7261 agtaatggat ctttatctga aacaaaagaa ccagcgagaa gattatcttg aacaattatc
     7321 tgggaaattg gtcaaacaat atgactactt atttgtagaa tcttttccaa aagaagaggc
     7381 acatgctgat ttttctattc acgactggca taaattaata acaaaattgc gttataagtc
     7441 ccaatggtat aataaaaaat ttcttttgat caataccgac ggagcagaag aaagcaattc
     7501 cgtaagaaag agccaagtag tagaaaaaat gggcagacat tcggttatca agggataaaa
     7561 aatgaatgaa actgatagga gcgttagggt tagccttgtt aataaaaagt cttaatgact
     7621 tttgtttcta ggaatttttc ttttgaagaa aagctcacta attatgacaa accaataaaa
     7681 caatagcaga aaaagttcgt acgagctttt tctgctattg ttaatttttt attcagaaag
     7741 tttacgaata catcaaaatt ctgaaacaaa aaacgtaact cacaaagagt tgtttttgcg
     7801 ttttttcttg aatgattagg tctgaatctt ttgctcacaa ccttattcat ggtgccccgg
     7861 cttgagctga tagatttttg aaggacggcc gattcctaca ggacgttcac cgatctcatc
     7921 gaaatattgg agcatagctt tcttaaaatt ggaatgatcg atcgttttat aatcgatccc
     7981 taagaattta gcaaatactt ttcgagcttc cgtaatcgtg aaatctttac ctaaaacttg
     8041 tagaacttgc ggctcgtgtt ccattttatt gactacacgg ttgaatgctt tcaagatgat
     8101 ttgactatgg tcaaaagcca aagtatcttt gccagaagac tctccagtga ctaaatcaag
     8161 cgagatagcc acttctccgc tagataaatg gatcttgtta ctatgacgtt ctagtgtgaa
     8221 ccagcgtact tccttggcat catctccggc gattagcggt tcttctccaa taaaggctaa
     8281 gtagctgact gtgacgaccc agcctcgcgg atctcgatta ggcgtactga atgtatgcag
     8341 ttgctcaata ttttcttttg aaataaccac accagtttct tcctttgttt ctcttaagac
     8401 gctttctcca gttgactcat ccttttggac aaagccacca ggcaatgccc atgaatttcg
     8461 ataaggatgc ccttttcttt ggatcaacaa aactttcagc tgatcttctt ctttgttata
     8521 gcacatcagt acgatatcga cagtcaaaga gggcttgtca tattctggac gttcctgttt
     8581 ttgataccag ttaagaaatt cttcttcact tgctttatgt tcataatata atttttcttc
     8641 ttctttagaa gcaaaacggt tcatctgacc acctccattg acagtaaaag tactttttac
     8701 cttattgtag cagactctga aaaaaaacca aggatttact tgaaaaatct aggttatcat
     8761 ttaaatatga aaaataaatg gaagtatgag attgaataaa aagaaaagca gataaaacgg
     8821 caatacttta taccgctctt cacgaatggc aaagaagcca caaataacga tatatacgtc
     8881 aatgataaaa cgcaaaatcc aagctgctgt cataaaactg ttcaaatgcc catggaggtt
     8941 aaaataacca tacatacgaa tataaaaata gctgaatatt aaatgtgcta atgaaagaaa
     9001 agcaatacta agtgtaaaaa aaagatgttt cttctgcatg attgttccag tcccttacta
     9061 agatagatag tctttattat accacgtgtg aggggagaaa taaaaagccg ttttttagta
     9121 gatatgaaac aaaacaaaaa cccgcattta ttcaacctat tcatcctttt aaaggataga
     9181 ttgaacgtag atagaagtcg tttatccagt ctttttcatt ttgaaaaaaa gctaacagca
     9241 tgcttttttt atggtatact agcaaggaaa cgaattcgca tcatgcgata aaggaggaag
     9301 agccccgtgg cagaaaagga aactttttat atcactacgc caatctatta tccaagcggc
     9361 aaacttcata tcggcaactc gtatactacg attgcatgtg acgcaattgc tcgttataaa
     9421 cggttgatgg gctttgatgt gttttatctc actggagtgg atgaacatgg tcaaaaaatc
     9481 gaaacaaaag cagaagaact aggtgtaaaa ccgcaagaat atgtagataa aatggcggct
     9541 gatgtcaaga aattatggaa aacgcttgat atcagttatg ataaattcat tcgcacgaca
     9601 gatgattacc ataaagcagc tgttcaaaaa atatttgatc gtttattaga acagggagat
     9661 atttacttag gtgaatatga aggctggtac tctgtttctg acgaagaatt ctttacagaa
     9721 acacaacttg cagaagtgta caaagatgac gaaggtaacg tgatcggtgg taaagcgcca
     9781 agtggacacg aagtagaatt ggtaaaagaa gaatcatact tcttccgcat gagcaaatac
     9841 gcagaccgtc tattggaata ctatgaagaa catcccgaat tcatccagcc agaatctcgt
     9901 aaaaacgaaa tgatcaacaa ctttatcaaa ccaggattag aggatcttgc agtttcacga
     9961 acaactttta cttggggagt accagtcaag agtgatccga aacatgtggt gtatgtatgg
    10021 atcgatgcat tatccaatta tattactgca ctaggatatg gctccgaaga tgaaagtcta
    10081 ttcgaaaaat actggcctgc agatgttcag atggtcggta aagaaatcgt tcgctttcat
    10141 acgatctatt ggccaatcat gctgatggcc cttgatttgc ctctacctaa aaaagtattt
    10201 gggcatggct ggctattgat gaaagacgga aaaatgtcta aatcaaaagg aaatgtcgtt
    10261 tatcctgaaa tgcttgtgga acgttatggt ttagatgcac tccgttatta tttgcttcgt
    10321 gctgttccgt ttgggtcaga tggtgtcttt acgccagaag attttgtttc ccgtgtcaat
    10381 tatgatctgg caaatgatct agggaactta ttgaatcgaa cgattgctat gatcaacaaa
    10441 tattgtgatg gactcgttcc tgactatgcc tcacttgtca ctccatttga tagtgaactg
    10501 tctacgacgg ctgctaacgt tgtcggacgt taccatgatg caatggaaaa aatggaattc
    10561 aatacagcat tggctgaaat ctgggtgctg atttctcgtg caaacaaata cattgatgaa
    10621 acagaaccat gggtattagc aaaagacgaa gaaaagaaag ctgaattgaa tagtgtcatg
    10681 atccatttag ctgaatcttt gcgtatcgca gcgatattac ttcagccaat catgacagaa
    10741 acgccagaga agatcttcaa tcaactagga ttggactctg aaacaatgaa tcttgaagga
    10801 ttacactttg gcgaatttcc ttcaggaaca aaagtagttg caaaaggtac accaatcttc
    10861 ccacgtttag atatggaaga agaagttgct tttattcaag aaaaaatgtc tgaaggtact
    10921 caaacaaacg aagatacagt caaatgggat ccagaagaaa cagaacttgt ttcaacgaaa
    10981 gaaaaacaaa tcaaatttga tgtatttgaa aaagtagaac taaaagtagc agaagtcatc
    11041 aactgtcaaa aagtagaagg ggcagataag ttgctgcaat tccgactaga tgctggagac
    11101 agccaagacc gtcaaatctt gtcaggaatc gctgaatttt atcctgatcc aagcgaattg
    11161 atcgggaaaa aagtcgtcat tgtagcaaat ctgaaaccac gaaagatgcg tggacaaatc
    11221 agtcaaggaa tgatcctttc tgcagaagca ccagatggct ctttacaagt aatcgaagca
    11281 ccaaaatcca tgccaaatgg ctcagaaatc gcatagaaat tcaaaggggg tatgacacta
    11341 gttgtgtcat accccctttt tccgaaattt gagaagcaat tttcgtaaat tccttcttat
    11401 tacttgaagc caatcatata atacagaaac agagaactat ccaaaataaa tcctagaaaa
    11461 agaagtacga gcttcgctga tttttgttaa tttagaataa tactaatagg aatcgtaaaa
    11521 acagaaaaaa agcataatga taaagggtcg ggcagatttt aattgattca tctggtactc
    11581 ttttttattg tccttacatt cataaagttt gaatgcaatg actttattac atctatattt
    11641 atatctaaca gtagcgttga tgtgttaatt ataagttcta tcactttatg ttcaattagt
    11701 ttgtgctctt atatgtgtga ttgaaaatat agataaaaaa acaatcatcg gctttgactt
    11761 cgatgattgt tttctttatt ttgtaaaaat cctatcactt gattttatgg taacctttta
    11821 tttttgtctt caaacggctt ggtaagagag cagtaagcag tgcaaatggg atcgaaaaga
    11881 cataccagaa aatcgtacgt ccgataccat aatgttgata gagctttgtc aacatcacgt
    11941 gtaacaagat caaccctaaa agcaaggagc cagctataaa ccaatgatta tcccaatgca
    12001 tgacttcact aagtgccatc actccaaaag gatagaaata aaaatagatc ccgactaagg
    12061 tcatccgcca gttctttgta aatgttccga tattcaataa aaagaaaatc gctgaaagca
    12121 aaataccgat aggcggaaaa agcccaacca aaaaggaata caacatcccc cacaagatca
    12181 tcacaggacc tttgatcaat gcaacgattg cgatgaacaa agtcagcaat attagttttt
    12241 gtgataacgt tgcaacagtc attaatccaa ataaaattaa tgtgaaccaa tgccaatggg
    12301 actgctgttg ttctttacgg gaaaaatcga cccggttgga taaataatct gctaatttcg
    12361 gataagaacg cttaaaatcc ataaactgtt cctccttcgt ggaaaaggta ggtttagtat
    12421 acgaaaaata aaaaagaaat acatccgtct taagagggag tttttcactg aaagtggaga
    12481 aaaaagaatg gtaaattaaa atattctcag ttatactgaa aattatacca aatttcaaag
    12541 aagaaagagg atgggaatga aaggcaaact aaaacgagaa atcaatctgt ttggcgcatt
    12601 agctaccgtt atgggaaccg tgatcggtgc aggcgtcttc ttcaaaactg ctgctgttac
    12661 tgctagtact cagtctacaa gtttgacatt gctagcttgg cttttgggtg gtttcttaac
    12721 gatctgtgct ggattgacag tggctgaatt agctacagca atcccggaaa ctggtggagc
    12781 tgtaaaatat attgaagctg cgtatggaaa attgcccagc tttttattag gatgggcgca
    12841 aagcttgatt tactttcctg ctaatattgc tgcattatcg attatctttg cgacacaatt
    12901 gacgaactta ttacaattat ctaccgatta tcttttgttg attgcagtga tcaccgctgt
    12961 ttctgtgaca gggttgaatt tgttagggac aaaagtcggt gcttctgtac aatcagtgac
    13021 gttgattgtt aaattaatac caattgctgt gattgtcata tggggattat tgaccccagg
    13081 acaaggaact gtccagttat ttccaatcga agctggaaaa gatgttactt ttgtcgaagg
    13141 attgagcagt gcgttgttag caacgttgtt tgcttatgac ggctggttag gtgtcggtgc
    13201 aatggcaggg gaaatgaaac ggccggaaaa agatttgcct aaagcaatta ttcttggttt
    13261 gagttttgta acagtcgttt atctattgat caattttgtt tttttgaaaa ctttgccgat
    13321 tgatcatctt gccggtaatt tgaatgcagc ttctgaagca tcagacgtca tttttggagg
    13381 aatcggtgga aagctggtca ctattgggat cttgatctcg gtatacggtg cattgaacgg
    13441 ttatacacta actgggatcc gcgtgcctta tgcgatggct ttagaagacg agctaccttt
    13501 cagtaaacag ctgacaaagc tttcaaagaa gtttacggtt ccttatgttc ctgcggtttt
    13561 ccagttagca gtagcttgta ttatgatgag ccttggttct ttcgactttt taacagacat
    13621 gttgattttt gtgatgtggc tattcagttt actgattttt atcggtgtct ttattttaag
    13681 aaaaaaagcg ccggaattgc cacgtccata taaagtacca ctttatccaa tcgttccgat
    13741 tattgcgatt ctcggtgcta ttttcatttt aggtatgacg atgatgacgc aaacgaaatt
    13801 agcattgatc ggtataggtg ttaccttgat aggtatccca gtctattatc aaaaaaagaa
    13861 aaaacgctca gaagagtaaa atatttcaac aacagacaga aaaagtacga actaggtcct
    13921 ctagatcttc gtgctttttc tatttcaata aaacaagttc ttctattttg atttttgcta
    13981 cttctgcaag tagtgcttat gtgctaaagt aagaatgaaa aggagagata aacatgatat
    14041 ttgattctca tacccattta aatgctgaac agttcaatga agatattcca gaaacgattc
    14101 agcgagcaca agaaatgggg gtaacgaaaa tggcggtcgt gggcttcgat acgccaacga
    14161 ttgaaaagtc tttgcaatta agccatgact atccaaatat atacagtatt atcggctggc
    14221 atccgacaga ggctggcagt tatacgaaag aaatcgaaaa taaattacaa gaacaattga
    14281 ctttaccaaa ggttgtagca ttaggggaaa ttggcttgga ttatcattgg atggaagacc
    14341 cgaaagaagt ccaagataaa gtatttcgca gacagattgc tatcgcaaaa gaaatgaacc
    14401 taccaatcag tattcatact agagaagcac ttgaagacac gtatcaaatc ctaaaagaag
    14461 aagatgttcg agatatcggt ggaatcatgc atagtttcag tggtgattat gaatgggcaa
    14521 aacgatttct tgacttaggg atgcatattt catttagcgg tgtcgtcact ttcaaaaaag
    14581 cattagacgt ccaagaggct gcagcaaatg taccaatcga acgattactt gtggaaacag
    14641 atgcccctta tctggcacca gttcctttta gaggaaagcg aaatgaacca ggctatactc
    14701 gttatacagt agaaaagatt gctgaacttc ggaatcttcc gacagaagaa gtggctgccc
    14761 aaacatggac aaatgcgcac cgattatttg gtctaagcga acatggataa gatgaagatc
    14821 caagagatta tagtcgttga aggaaaagac gatacaagaa ggctgcggga agtagttgat
    14881 gcagatacga tagaaacaat agggtctgca atcaatgaag agatcctgat gcaaattgaa
    14941 catgcccaag agactcgtgg agtgatcgtt tttaccgatc ctgatttttc aggagaaaaa
    15001 ataagaaaaa tcattatgga agctgttcct caggcaaaac atgctttttt gccgcgcaca
    15061 caagctaaac caaagaaaaa gggaagcctt ggtgtcgagc atgcaagtga tgctgctatt
    15121 ttagaagcat tgaaaaaggt cgtgacgcca gacatttccc aaacggctgt ttctgaaatc
    15181 actagacaag accttttaga ctatggttta atagctgggg cacaagcaaa agaaaaacga
    15241 gaaaaactag gtgacgagtt gcgcatcggt tacacgaacg gcaaacaatt ggaaaaacgt
    15301 ttgaagatgt ttcgtattac gccgaaagaa ctcgcccaag caatgaaaat agtggaggaa
    15361 caacatgagt gaatatcgtg aaattgcgac accctctaga acaaaagaga ttttattgaa
    15421 acatggtttt tcatttaaaa aaagtttagg acaaaatttt ctgactgaac caaatatttt
    15481 aaggaaaatc gtagaaactg cgcaaataga tgaacatacg aacgtgatcg aagttggacc
    15541 aggaatcggt gcattgacgg aacaattagc caagcatgca aaacaagtcg tagcatttga
    15601 gatcgatgat cgtttgatcc ctgtattggc tgatacgatg caaccctatg acaatgtgac
    15661 tattgtacat caagatattt taaagaccga tttgtcaaca gctgttagag aaacatttca
    15721 tgaggagctt ccgttaaaag tcgtagcgaa tttaccttac tatattacga caccgatcat
    15781 gatgcatttt cttgaatcag atttggttgt ggatgaacta gtcgtcatga tgcaaaaaga
    15841 agtagctgat cgtatctctg ctgaaccagg aacgaaagct tatggttcat tatcgattgc
    15901 tgttcaatat tatatggaag ccagtttagc attcatcgta ccaaaaacag tatttgtccc
    15961 tcagccaaat gtagattcag cgattttgaa actgacaaga agagatatac cggcagtcga
    16021 agtgacagac gaaaaagcat ttttccgatt gacaaaagcg gcatttcagc aaagaagaaa
    16081 aacgctttgg aataacttgc agcattcgta tggcaaagat gaccagacaa aagcatggtt
    16141 agcaaaaagc ttggaaacag ctggaatcga tccaaaacgt cgtggggaga ctttgtcatt
    16201 acaagaattc gccgcattaa gcaatgcgat gtcagaaaat atgcagtaaa aaaagaggtt
    16261 gtgacagaag ttgtgaaaaa gctgtcctgc atcaagtaat aagaacagcc aaacaaaaat
    16321 agttcctcat gtttttcgtt tgtttgggct tattaccgca gatgcaagct tttgaacacc
    16381 gtttaagaaa gaggttgtgg gcctgccgtt taactctaag tcacaaggaa caatttcaaa
    16441 aaacagcttc tcatgttttt gagttagttg gtcttattac ttgatgcaag cttttgacca
    16501 ctattatgcc gaacaaggag atgtgacaca aattttgtca catctcctat ttttgtttct
    16561 tgatcaaaaa attcttatac ttgatactca cactcatttt gtatggattt agaaaagaat
    16621 atgtgtcttg acagttatta gtaagtgagt tatactaagt cgacatttat gtgtcaagac
    16681 atattaggag agagaaaaaa tgagaaaaaa ttcagttcga aaactaacga tttctgcttt
    16741 attgattggg atgggggtca ttatcccaat ggtcatgcct aaaatcatga ttccaccagc
    16801 ttcctttact ttggcgtctc atgtgccatt atttattgcg atgtttttta caccaggagt
    16861 tgcagtggct gttgcgctag gtacgacatt tggtttcttt ttaacaacgc cggttatcat
    16921 cgcattgcga gcattgtcac atcttgtgtt tgcgttgatt ggggcttggt acttgcaaaa
    16981 gcatccgagt atcgttttaa aaaatggaca gtttactttt gctaattggc gtttccaagg
    17041 gttcaatttt gtgatcggtg tgattcattc tttagcagaa atgctagtag tcagcatctt
    17101 ttactttatt ggtaatatgc ctgaaacata ttattctcaa ggatattttt atacagtatt
    17161 catcttgatg gggattggtg ggcttatcca cagtttagtt gattataata ttgcttattt
    17221 tatagcagga acactcagca aatattttga tatccccgta tttacggcag caaaaaaaga
    17281 aaatcggaaa gagtcgattc aagggtacgc gaagaaaacg acatattaga catgatctct
    17341 caaaaaaaaa actctcatct agtaatggat aataaatcct ctaagatgag agttttttgt
    17401 cctaaaaaga gctgcaccaa catcttctgg cacagctcta acagggggca atttatggaa
    17461 gtttgatgac ttgttctgtt tctttatcaa agaagtggcc tttgctgatg ttgaaggcaa
    17521 ggtcgactat ttcgcctggt ttatggaaat ctcttgcatc tacttttgag atgaactcag
    17581 tagaaccagt ttttgtgtaa agcatcgttt cagctccaag taattcggaa acgacaactt
    17641 ctgatttcac tacggcttca ggtgaagctt caagggctac ttgttcactg tggatgtctt
    17701 ctggacgaat accgaagata acttcttttc cttcataacc tttttcaact aatactttgt
    17761 tttttccttc aggaatacga agcttaagcc catgtccatc tgtgataaca ccatttttca
    17821 atgtcacatt aaagaagttc atggcaggag atccaataaa gccggcaacg aacatattga
    17881 cgggtgtatc ataaacttct ttcggtgtac cgatttgttg gataaatccg tctttcatga
    17941 taacgattcg gtcagccata gtcattgctt ctgtctgatc gtgagtaaca tagatggtcg
    18001 ttgtttcaag acgctggtga agtttggcaa tctcagcacg catggctaca cgtaatttag
    18061 catctaaatt tgataatggc tcatccatta agaagacttt tgcatcccga acgattgccc
    18121 gtcccaatgc cacacgttgg cgttgtccac cagataaagc agctggtttt cgtttcaagt
    18181 attctgtcag acctaagatc tctgctgcat tgtccacacg ttttttgatg tctgctttat
    18241 catatttacg taatttcaag ccaaaagcca tattatcaaa aacagtcata tgaggataca
    18301 atgcgtagtt ttggaaaacc atggcaatat cacggtcttt aggtgccacg tcattcatga
    18361 cttttccgcc gatatttagt tctccttcag agatatcttc cagtcccgcg atcattcgca
    18421 aagtagttga ctttccacaa ccggaaggac caacaaaaac gataaattca cggtctttga
    18481 ttgatagatt aaaatcagtt acagaataat tttctgcatt gtcatatttt ttatatacgt
    18541 ttttcagagc catttctacc ataatacgtt ccacactttc ttttttagtt ttattaacta
    18601 tttaaagtat aaatgaaagc gttaactaac ttcaatgtca tgttgaacaa gaattttttc
    18661 ttttttttgt caatgtgcta gttttaagct ttaataaata tccataagaa ataaaaactg
    18721 atacactgga gtaaataagt atgttaacaa tatcaaaagg agtgtctctt atgaaaatcg
    18781 ctttgatcgc tcatgaccga aaaaaaccta tgatggtgaa attagcaaca gcgtataaaa
    18841 caatattgga aaaacatgaa ctttttgcta ccgggacgac tgggcagagg atcacggaag
    18901 caaccggctt gccgatccat cggtttaaat ccggaccgtt aggcggggat cagcaaattg
    18961 gagccatgat ttcggaggat cagttagatt tggtgatttt tcttcgagac ccgctggctg
    19021 cacagcccca tgaaccagat gtaacagcac ttatccgttt atctgatgtc tatgagattc
    19081 cgttagctac gaatatcgga acagctgagg tgctactaag aggactggaa gccggcttcc
    19141 tagattttcg aagaatcgtc catgaaatag acaagcatcc actttcattt tgatggataa
    19201 gcgtatggaa aaataacgtt taatgttcgc ctatatgtag ctataaaagc taaacaaact
    19261 gataatatgc gatataatac gaacgtattg gataatataa acaataatat tagtttttcg
    19321 tatttgcagt aaatgtgaaa atatgctata gtttctgtgg gaaaagatcc caaaaataat
    19381 acagagtaga aaataaagcg ttaagtgcta agaggatggg atgttgcctc ttggacgaag
    19441 gacgtaatgt tcgcggtttt attttcgcat tcgctgcatg atacgatgta gcagctctta
    19501 aaggagatgc taaaaatgaa gaaacaccgg ttagacgcac gtatgattgc tattatgggc
    19561 ttattgattg cattgatggt cactttgtcc cgactggtag caatcgagac cccatttatt
    19621 aaaatcagtg tcacatttat tccacaagtg atcatgggca ttctttttgg tcctttttgg
    19681 tcagggatag gcgcagtttt agctgattta gtaggaatgg ccctcttctc gaaatctgca
    19741 ttctttattg gatttacatt gaatgcattt attgaaggag caatctacgg attctttttc
    19801 tatcgaaaag agatcacttg gaaaaatgcc attctggcta cgcttagtgt cacgctgatc
    19861 atcaacttat ttttgacacc attatggctt gcgttgatgt accatgtgcc attgttcagc
    19921 tgggtcgttt gggcgccccg cctgttaaaa acagtaatat ggttaccgat tcaatcaatt
    19981 gccatttatt acgtaggacg tagtattcct tacaaaaaaa ttttaagaag tttagcgatc
    20041 cacgctaaat aaaaaacaag cagtcttccc atcccagaag actgcttgtt tttattgtgc
    20101 tgtgaattgt gcttgataga gccgtcggta ataaccatct ttttgaagca gctcatcatg
    20161 ggtgcccatt tcgacaatag ctccctgatc catcactaat atctggtccg catttttgat
    20221 tgtggacagg cgatgggcaa tcacaaaact ggtttttcct tccatcattg ctaagaaggc
    20281 ttgttggatt tttttctctg tcaatgtatc gactgagctg gtagcttcat ctaagatcaa
    20341 cataggggga ttactgatca tcgttcgagc aatcgtcaac agttggcgtt gtccatctga
    20401 tatctttaac ccttgaccgc caatcttagt atctaatttt tccggcaaac gttctacaaa
    20461 ttcatacata taagaggctt ttagggcatc attgatttct gcgtcacttg cttccggatt
    20521 cccaaaacga agattttctc gcaatgtact gtcaaatagc caagtatctt gtaacaccat
    20581 tccaaaactt ttacgtaatg tatcacgttg gatagagcgg atgtcatggt catcgactgt
    20641 aattgttcct tttgttgtgt cataaaaacg catcagcaaa ttaacaagtg tggattttcc
    20701 ggcccctgtt tttcctacta tagcaatggt ttgacctgga taagcagtaa aattgaaatc
    20761 ttcgatcaaa cgctgcttgg ggtcataaga aaaagaaaca tgatcaaaag agacttttcc
    20821 tttgatattt ttgggttcca aggcataggc atctggcaaa tcaggctttt cttctggctg
    20881 atcgatcaaa tcaaatgctc gttctagacc agaaaaagcg gtttgcagtt gtgtcgtgat
    20941 tccagaaagt tcgataaatg gtttagaaaa ctgacttgag tagatcgtaa agctggaaat
    21001 cacaccaaca gtgattgacg aaccactctt caatgccaat aggccgccaa ctaaaccaat
    21061 cgataaataa gcaaggtgat ctacaaaacg agaagacggg tttgtcaagg aagaagaaaa
    21121 ttgtgcccgt tgtccttttt catatagatc agcatttagc agttcaaatt gctgttgact
    21181 agcttcttct tgctgaaaag ctttcacgat tttctggtta ccgattcgtt cggtcacaaa
    21241 gcccgagata tccccaataa tcctttgctg tctatcgaaa tttgtttgtg aagcacgagc
    21301 aacgatccaa ctcacaagga aaataattgg tgtagaacaa agaacgacca atgtcagaag
    21361 tggacttaga cgcatcataa aaataaacga aagaatgatg attgccactc cagaaaataa
    21421 ttgattgaag gccgcagaaa ttgcaatgga aatattgtcc atatcatttg taaatcgact
    21481 gacgatattt ccgtgggagt tttgatcata ataacgaagt ggcaatcgat tcagatgagc
    21541 aaatgcatct tttcttaatt ttgcaacgga taagtaagct aaccgattgc ttaacacttg
    21601 gatgagccat tgactgacta cgttgattaa aacgatacca ccgaatagaa taagtaaatg
    21661 gaacagtcga ttgaagcgga cctgtccttc tccaatcatt tgatcgatac tttgaccaat
    21721 ttgtaacgtc atcacaactg ttgcaatccc attgatgatt cctagtatca cagctagcca
    21781 aatgtctttt gcataggcag taaggtaagg aatgaatcgg cggagcgtct gcaaggataa
    21841 tttttttact ttcataacta ttcttcctcc tgtgatgcga cgatttcctg atattctgct
    21901 gattgagcaa gtaattcttc atgggtaccc tgagcagcaa tcttaccttg atccatcact
    21961 aagatatgat gtgcttcttg aatagagcgg acacgttggg agataataat cactgtactg
    22021 ccaagataag tctgtagcgc cttacgcaaa tccaagtctg tttgataatc caacgcactt
    22081 aaagagtcat ctaaaatcag taatgctggc ttggcaatca aagctcttgc aatcgtcagt
    22141 cgctgttttt gtccgccaga gaaatttttc ccattttcca tgacttttgt atctaaacca
    22201 tttggaagct gacggacaaa ttcttctgcc tgagcgattt tcagagcttc ccaacattct
    22261 ttttctgtgg cgtctttctt accccattga aggttttcac gaatagtccc ggtaaatagt
    22321 acagaagttt ggggaacaag agcaatttgc tgacggagat gatccagctg ccaagaacga
    22381 acatccattc cgtccattat aactgtaccg gaactgacat catagaaacg cggaatcaat
    22441 tgtgtcaaag tcgttttccc gcttccggtt ggaccaatga ttccaaggac tgtatttcgc
    22501 ttcagctgaa atgtaatatt ttggagagaa agaccagaat caggagtata acggaagtca
    22561 acatggtcaa aagcgataat tgcttcagaa gaaaaatcgg gagtagtact gacctcagct
    22621 tcaagaatcg aattttctgt gtctagcacc tctttcaccc gatttcctga agcttcggca
    22681 cgtgtgaaga tcacgacgag gtttgaaaca acgatcagtg ccaaaagcat ttgattcata
    22741 taattgatca aagcaagaac ttgtccttgt tgcaagctac caatatttac tttgatcccg
    22801 ccaactgtca gcaaacagat gatcccgaca ttcatgatca aggttgtagc aggtgataat
    22861 agggcagaga tattggaaac acgtaaataa ttgttcgcta gatcatttgt gcttttatcg
    22921 aacttctctg tttctttttt tgtctgcgca aatgcgcgga tgacacgaac accgctaaga
    22981 ttctgactaa cgtgttcatt gagctgatct agtttttctt gcactttttg gtagagagga
    23041 acggtttttt tgatgataaa aaataaaatg attgagaaaa taggcaataa tgctaagaaa
    23101 aacagaccta cttcccagtc gatcacaaaa gccatgacca ctgacccgat acttaaaaac
    23161 ggtgctctga tcaataaacg aatgaccatt gctaaagcta gctgaagctg attgatgtcg
    23221 tttgtcattc gggtgatcaa ggtatctgtt ccaaaatgat tcaactcgtt gtgagagaaa
    23281 gtattgattt ttttgattag ctgattacgt aactctgtac caaatccttg tgaagctact
    23341 gaagcatagt attggcaaat gatagcacaa atcagaccaa tcacagacat agcgaccatc
    23401 cataagccca tttcaatgat ttttcctcgg tcattcattt ttagtccatt atcgatcaag
    23461 gaagccatca ttaaaggcag gacaagttcg aagacagctt caagaaattt gaagaacggg
    23521 cccaaaatga tttgcttacg atagtctttt gcataacgta ataactgaat catatgattc
    23581 ctcctattat ttatttgtcc acaattgaaa aaacaagtac taccacattg tagcttagga
    23641 aatttagaat gaaaagagaa caagcctttt cataaatgaa agttttatta tgaagaagct
    23701 ttagaaattg aatagactat tttttttaaa aaaaagaaaa tgagaaaaca tgccagtctc
    23761 ttcattcata ggaacgtcta ttttacttaa tttttctata aatgaattct gcataaaaaa
    23821 tatatcaagt aaatgtttat agatgaaact ctatttactt cttttttaat ttgttcttgc
    23881 aatctttttt gctacgtaat ttttgtaaaa aagtgggttg agagaaatct aattgatttt
    23941 caaagaagga gtatgaaaat gagaaaaata tttattcgtt tattgtgggt actatcttag
    24001 aggaataaat aatagtggag aaaactatgg tttggcgcag attctatcag gtttgatgga
    24061 acgaagacaa cctcccgaca tcaagtcaaa agctacttga gattttgatc tgaaaataaa
    24121 aaaagaaaac aaggtaggaa catcaatgtt tctaccttgt tttcgtaatc aatgggaaaa
    24181 aactaagagt ggtgtacgta atcaataaaa aggatggtga caacagtacc tctttactct
    24241 ctgaaagcat cataactcgt ttttcctcat tatgccaata aattgttgtt ataccacata
    24301 taaggatttg taatagctaa aaacagacag gaaagttgaa atctgtgaat cttattttgt
    24361 atggaaggat ttatagaacg caaaaatcaa gataaagatg aataaaaatc ctgtttcggc
    24421 tatatattgc gtatctacgc caataaaacg agtttgttct gtcatagcca tataaagcaa
    24481 aggcaaaaat acaactacag cgagataaga tccgatgatt tctcgaaaag ttttccagga
    24541 agtatgtttc ctgtttttat atttatccga tttcattgaa tagtcatagc ctccttattc
    24601 tttcatatgt tgaaattcca gtataaaaac tggaaggaac tgtacgtttt ttagaataaa
    24661 aattataagg gataagtagg ctgctgtcta taataagata tatttttcaa ttctttatta
    24721 aagattttat tgacaaaatg aatccgtttt ctatgatgga ggaggggtga ataagttgtt
    24781 gaagaaggaa attttatgtt acttagagaa ccagacagcc tttttcgatt tagaacatat
    24841 gaacgaggtc tttacagcac aaaggttagc tgatcatttt ggggtaaaaa gaaacacagt
    24901 cagtcattat ttgaaccagt taaatgatga gcaactacta gtcaaaatca attctcgacc
    24961 agtgatttat ttccacaaag aagcttttga aaaacaattt tttaagttac gcacgaattt
    25021 ttatttttct atcgatgatc tcaaagagga acagcctttt ttcgcgcaac caaaagactt
    25081 attctctttt atgatcggat acgacgccag tttgaaagaa agtatcgaac aaatcaaaac
    25141 agctctttac tatccagatg gcggattacc acttttgatt acaggcgaaa gtggcacagg
    25201 taaaagttat ttagttaatt tggtctatca gtattgtctg cttcatgatc tgctagaaga
    25261 ttctgcacca ttcatcactt ttaattgtgc acaatatgca gataatcctg aacttttgac
    25321 tagcaatttg tttggacatg ttaagggagc gtacactggt gcagaagaaa ataaaaaagg
    25381 ggcttttgaa gcagcagatg gtgggatctt gtttttagac gaggtacatc gcttaacacc
    25441 agaaggtcag gaaaaactgt tcacctatct tgatcaaggt gtgatttatc gtatgggaga
    25501 acctaatttg tcacgccgga tcaaaacacg tctctttttt gctacgactg aagagattac
    25561 tagtcatttc ttaacaactt tcattcgaag aatacctatc cgaatcgaac tacctgcact
    25621 taatcagcgt agtcgtaatg aacggctaga actgatttac tctttttttc tcgaagaaca
    25681 aagaaaaatg gggagccctt tgtcagtcag tggaccagct ttgtcacttt taacaagtca
    25741 gaaattatcc ggaaacatag gagagctgaa aaatatcgtc aaagtttcag cagcaaaagc
    25801 atatgctgaa cagcgtgaac agcctgaaat ccatgtcacg atccatcatt tgcaaaaaga
    25861 actacttgcg cagcctatta ctaaaaatac agcacagcaa ggaatctata ttacgcaaga
    25921 ccagacctta gagcaattaa tggagaagca acagcccgaa cagcagcgaa tcgttaaaag
    25981 ttttgagcaa atcctaattg atttcaagaa aaataactgt ctgctatatt cttgcgaagg
    26041 aaaattaaag caagaagtga ttcaattatt tgattttttg ctgtttgaga ccagtagaca
    26101 agaaaaacac gagctgctgg tttattggac acaatctatc cgagagacct ttcgacaaat
    26161 ggaaactgcc tatcaaatca aattcgatgg aaacagtgtc tatgccttga gttactatct
    26221 ctatcagcga agcactatca aatggttacc ggaagaacat gatattatcc aactaatcaa
    26281 agaattagaa caacaagttt ctcagtctta tccatctagt tatcactatg tccaacgaat
    26341 tctggaatta agtaagatca aattagatat tgaagacaca agtatggatc ggattttact
    26401 gacgatctat ctaaaaaaag cgaaatggtc aaaagaaaaa cgactgccaa aagcgatgat
    26461 tgctgctcac ggttatgcaa ctgctagcag tatggcaaat gtagtaaatc gtttattaca
    26521 agaggatcta tttgaatcat ttgatatgcc actagatgtc acaccacagc agattgcttc
    26581 agaaatcctt gattattgcg aaaacagcga tgtttcaaat ggattgatca tattagtaga
    26641 catgggatct ctgaaagaaa tccatcagtt ttttaaaaaa cagttatccg taccgctttt
    26701 gattttgaat aatgtgacga cacctttagc aatcacagta ggggaatgcc tgcaaaaaaa
    26761 tgtatcatta gaagagatag cagaagagac tgttcgacag atccagccag aatggcgact
    26821 gctctatcca gaggaaaaca aacccaaagc actcattact acttgcttca ctggaatcgg
    26881 tacggcggtc cacttaagtg aactgctaga aaaaagctta cctacggctt gtcagttgaa
    26941 aatcatccca tatgaatatc agcaattaaa agataagaaa aacagtgacc cattattttc
    27001 catatacgaa gtcgtaggaa tgattggcac cacagatcct gatatcacca atatcgacta
    27061 tctttcatta gaagacctca tctcggggga aaagatgtct gtactagctg aatggttgga
    27121 cactactatg aatgcttcag agaaggaaat attcaatcag cgcatcattc gtaatttttc
    27181 attggaaaaa gtactggatt ccgtgactat tctagataca gagaaggtga tgggagaaat
    27241 cgatcatttt atgagagaat tagaggtgca gttgaatcga agcatcagca atcccaaaaa
    27301 attagcgttg tatgtccatg tgagctgtct cattgaacga ttgatccgac aaatgccaat
    27361 cgaaacctat caaggattag agaaatggaa gcagtgtcaa aaagaagggt tatctgctat
    27421 taaatctgcg tttagtgtca ttgaagagca ttatagtgtc atgatcccca attctgagat
    27481 tgcctatatc tatgatattt tgagtgaaga tacagagttt ctctcgatag aagaagaatt
    27541 ttgaacataa gcaagttgtt gccactaatt ttggcatgca acttgctttt ttagtatgtg
    27601 taagtgaaaa gaggagatga aaaagtggat agagaaatta tcttagcttc ccatggaaaa
    27661 tttgcttcag ggatattaga ttcattagag ctgatctatg ggaaaaatca ttctattact
    27721 gtattcgatt gctatactga tgagaatttt gatttagcgg aaacagtaag gcagcttttt
    27781 atgaggtaca aagataaaga aatcattgtt ctgacggatc tttttggcgg aagtgtcaac
    27841 aacgaattct tacaatatat caatcgggat catgtctatc tcgttgccgg attgaatttg
    27901 ccactattga ttgagctagt atctcggttg gacgctgaag tagagacaac tgttttgatc
    27961 cagcaggtgc taactgattc aaaaaaaatg atccagtttt gtaacgagcg gctcaatcag
    28021 aaaatagaag aagacgaatt ttaggaggaa gaaaaatgat tgtattgaca agagtcgacc
    28081 atcggttatt acacggacaa gtagcttttt catggacaca aacaataggc gcagactgta
    28141 ttttgattgc taatgatgat gtacctacga atgagatacg aaagacaacg atcaaattag
    28201 ccaagccaca aggagtcaaa ctagtgatca aatcaattga tgactcgatt gccgcgttga
    28261 aaagcggggt gacagataaa tataagttat tcatcgtagt ggaatcgatc gaggatgcat
    28321 ataagctggc agaagcgtat ccagaaatca aaaccatcaa cttaggaggg atcaaggcaa
    28381 aacctggtac tcgcagcatc ggaaaagcgg taaatatttt accagaagag gaggaacgct
    28441 tgaaacgatt aattgacaaa caaatagaag tagaaatccg tcaagtacca gcagataaaa
    28501 aagtggatgt aagagaagtt ttgtaagaaa ggaagaacct caatgattgt acaagcagtc
    28561 ttattaggaa ttgttgcatt tgtcgcacaa tcagaatatg cactagggac atcgttgctt
    28621 tctcgtccga ttgtgacggg gctgtttaca ggaatcgtgt taggtgatgt aaaaactggg
    28681 atcattatgg gcgcaacttt agagttagca tttatcggat cgttctcagt aggtgcttcg
    28741 attccacctg atgttgtgac cggtgggatt ttagggacgg cttttgctat tacagctgga
    28801 gccggtacag aaaccgcgct tttactaggt ttgccgattg ctacgttgac attgatattg
    28861 aaaaatattt acttagggct tttgattcca attatgaacc ataaagcaga caattatgca
    28921 gaagaaggaa attataaagg agtcgagcat atgcacttgc ttgccggtat cggtctttcc
    28981 ttgatgcttg gtgttgtggt aatggtttct tatatggtgg gaagcaatac gatcggaaat
    29041 ctattaaata tgattcctga cttcgttcaa aaggggttgt cagtagctac agggttgatt
    29101 ccagctcttg gttttgcgat gcttgctcgt ttgctgatca acaaacaagt tgcaccatat
    29161 tttttcttag gttttgctat agcttcttac ttagagattc ctgtgaccgg agtagctatt
    29221 tttggtgcga ttttagcagt tgtggtagtc aatattatga atgtccgtca actaccactt
    29281 caaacaactg caggggaggt agaagaagat gaagaattct gagatcaaaa acagcaaaat
    29341 aacgaaaaaa gagctaaatc acgtattttg gcgttctttt caaatggagt tttcttggaa
    29401 ttatgaacgt cagatgaata tggcttatgt ttatgcaatg attccgattt tgaaaaagtt
    29461 atatcagtcg aaagaagaaa tggcagcagc tttgaaacgg catctagagt tttttaacac
    29521 gaccccacat atcgttacac tgattttagg aatcacggct gcaatggagg aagaaaataa
    29581 ggatgatcct gaatttgatg tgactgcgat cgatagtatc aaaacatctt tgatgggacc
    29641 tcttgcaggg ttaggagatt ctttcttttg gggaactttg cgattgattg ccacaggtgt
    29701 gggtacctct ttagccatgc aaggaaatat tttaggccct atcttgttca ttttgatttt
    29761 caatattccg cacattctgt ttcgctatct agcaacaggt tggggatatc gcttaggaac
    29821 gggttttttg aaaaagatcc aggcaaacgg tatgatggaa agtttgacat taggcgcgtc
    29881 tattattgga ctgatggtcg ttggagcaat gactgccacg atgatcacca tcaatattcc
    29941 tttaaaaatc ggttcaggag aaaatgctgt gactgttcaa agtatttttg atgatatcgt
    30001 tccaaatatc ttatgtcttg gagcatttgg catagtgttt tatctattga agaaagaagt
    30061 gaaaccatta acgatcttga tcggactagc tttctttgga atttttggtt cgtggatagg
    30121 gattttttaa aaaaggagca gagaaaatgt ttacgatgca agattatatc tatgaagaaa
    30181 aggaagtact aagtacgatc ttgaaaaaga atgatttttc gacaagagaa catatgaaaa
    30241 aaacagtgaa tttactgatc ttagcaacgg gttcatctta taacgcttgt ttagcagcga
    30301 agccagcatt ggaatcttat ggtgatctta ctgtcgatat tcaagaacct ttttattttt
    30361 tcaattatgg aaaactttct ccttcaatcg atactgtgat tgctgtgtca caaagcggga
    30421 aaagtgcttc tactgttgaa gcagtaaaaa tgatacaaaa acaagggtta cctgttgttg
    30481 ctatcacaaa tgacgtgcaa agtccacttg ctttagaagc tgcacaaatt attgatttag
    30541 gtgccggggt agaatcagta ggcttcgtta caaaaggcta ttctgcgact gttttacagt
    30601 tactactctt agggatagga attggaataa gtaaaaataa aatcagcaaa aaaatagaac
    30661 aagactacat gcaacagttg cgcaaaataa tcaaccattt accagcgatt attcaaaaaa
    30721 cggaagaatt ttttgatgga tatcaaagtt tatttcgttt agcacagcgt ttcgtcacca
    30781 ttggatatgg tccaaactgg ggaacagcta aagaagcaga gacgaaattg actgaaacaa
    30841 tccgcgttcc gtcacaagga tttgagttag aagcttatat gcacggccca tacctggagg
    30901 cagatgcttc gcatttgcta ttctttattg aaggggaaag tgtaaataag gaacgttcgc
    30961 aaaagttgca aagatatatg agtcggtacg ttggagagac tctcaccatt accacaaaaa
    31021 aagcaagaaa cgagaaaaca cttggattag caatagaatg tgatgaatat ctgtctgtat
    31081 tagcgcttgt cgttccgttt caactattcg cttataaaac cgcagtagct aaaggtattg
    31141 acttgaacaa aaagattttt gaagactttg atacagtctt gaaaagtaaa ttataagagg
    31201 tggaaatcat gctgaaattc aatgaagaag aacaaatcaa agcaataaaa ggcgcactag
    31261 ctttacgtcc gcaagtagaa gaaatcatcg ataaacttta tgcggaaaaa tttgacgctg
    31321 tctattatct aggaatcggt ggaacgtatg cttcctcgat gcaagctgtc acttatatga
    31381 atgggaaaag caacttgcct gtctttgtac agcatgctgc agaatattac actactggaa
    31441 acaaaagatt aacaaaagat tctatcgtta ttttgtcctc tgttaccggt acaactcaag
    31501 aagtagtgaa agcagtagaa caaatcaaga aagtaggagc gacactgatt ggatttatcg
    31561 ataaagcgaa cagtaagttg tctcagctat gcgattttgt ggtcacttat ccagctcctg
    31621 gaacagaaca aatcaaattt tttatggcag cagatcgatt gatgtatttg aatggagaat
    31681 tcgaagctta ttcagaatac tatgaacagc tcgaacgtta tttaccaaca gggttagtgg
    31741 aagcagagaa aaaagcagat gcatttggct tatcatttgc agaaaaacat cgacgtgata
    31801 gtatgcatta ttttattgga gcagggaatc aatggggagc tgtttattca tatgccatgt
    31861 gttactggga agaacaaagc tggcttccat cgaaatctat ccatgctgcc gagtttcttc
    31921 acgggacgtt agagatcgta gaagaaacaa ccccagtcac attattttta ggcgaagatg
    31981 aacagagagt gttatctgaa cgtgtggcta aacttttgcc aaaaatttgc agcaattata
    32041 cattgataga cacaaaagac tatccagtag aaggtatcag tgaaaaatat cgcggacgtg
    32101 ttttgtcttt cttattgatg catgtagtga cacaacgcat cgatgctcat gtggagcagt
    32161 tgaactgcca tccattagag atacgccgct actatcgtca gtttgattat taggatagac
    32221 tggataaaaa gaatataaaa aataaagaga gcagcgataa ctgctccctt tattttttta
    32281 aaatattggc atcaattacg ttataaaaca cattttttgc attatctaca gttcaactta
    32341 taatctgttt aatgggacat gtgggtgcca accatttttt gttatgcaat agtactattt
    32401 agttgttgca tggatccgta gtaaaagtcc aagtacaacg agttgcgcca gtttacatag
    32461 cgttttgtat ccagaatgtt gactaaaaca aaagttccta tgtcaaccgt caacagagac
    32521 aatctgtccg ttgcaggacc tgacagattt agaaatgttc aatctgtctc ttatgtattt
    32581 taaatcaata ctcactagga gaagtttcct aacctcctaa tagtaatgct ggtagtaaca
    32641 ttgtcaaaac tgcttcctct taggaacaag caaagcataa cgagtgtaaa aataaaatgt
    32701 agactattgt aacatatcat tgattatttc aagtaaaaac gataaaagaa gtaaacaaac
    32761 acttaaaaac aatggaaatt agtcccaact ttaagaaatg tatactataa tggaaattat
    32821 caattattta tttggaggaa tatccatgaa acaaaaaatc gcagttcttg gtcctggatc
    32881 ttggggcact gctttagcac aaacattggc agaaaacggg catgatgtcc gtatttgggg
    32941 aaatgtacct gaacagattg atgaaatcaa taagtatcat acaaatcagc attttttacc
    33001 tgatttgacg atcccagaat cgattatcgg atataaagaa ttggcagaag cagtggataa
    33061 cgcagatgct attttatttg ttgttccaac aaaagcgatt cgttcagtcg cacaggaact
    33121 tattggtaaa atgaatacta agccaatcat tatacatgca agtaaaggac tagaacaaga
    33181 tacgcataaa cgaatctcag aagttttggc tgaagaaatt ccagaagaaa aaagacaggc
    33241 aattgttgta ttatctggtc ctagccatgc tgaggaagtg gcagtacatg atatcacaac
    33301 aattacagct gcaagtattg atcaaaaagc agcagcctat gtacaaaaac tgttcatgaa
    33361 tgattatttc aggatttata caaataatga tgtgattggc gtagaaactg gtgcagcact
    33421 gaaaaatatc attgcaattg gtgccggcgc tatccatgga ttaggattcg gagatgatgc
    33481 aaaagcagca atcatgactc gaggattggc agaaatcagc cgtcttggtg tcgcgatggg
    33541 tgccaatcct ctgacattta tcggtttgag tggtgtgggg gacttgatcg ttacttgtac
    33601 aagtgttcat tctcgcaatt ggcgtgccgg taacttgtta ggacagggac ataagctgga
    33661 agaagttcta gaaaacatgg gaatggtcgt tgagggtgtg gcaacgacaa aagcagcagt
    33721 agagctagct caacagctag gtgttgagat gccaattact caaacaatct ataatgtttt
    33781 gtataatgga gaagatatca aaaaagcagc aaaagaaatc atgttacgtg acggaaaaat
    33841 ggagaatgaa ttttctcttc gttaaataga tgatcaatag ttaatcgt
//
LOCUS       DACKAC010000025        32968 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.025, whole genome shotgun sequence.
ACCESSION   DACKAC010000025 DACKAC010000000
VERSION     DACKAC010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32968
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.025"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            164..2698
                     /gene="secA"
                     /locus_tag="I0B46_001245"
     CDS             164..2698
                     /gene="secA"
                     /locus_tag="I0B46_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAR2083557.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDIQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGDE
                     DAVIQSKMLTKQVEAAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIVDFAGSTLVHEDSISLTDIEGKTSQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKDAN"
     gene            2825..3926
                     /gene="prfB"
                     /locus_tag="I0B46_001246"
     CDS             join(2825..2887,2889..3926)
                     /gene="prfB"
                     /locus_tag="I0B46_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAR2083558.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAVMQEEAEARI
                     NALDEKMNTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            4031..4717
                     /gene="ftsE"
                     /locus_tag="I0B46_001247"
     CDS             4031..4717
                     /gene="ftsE"
                     /locus_tag="I0B46_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAR2083559.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            4701..5594
                     /locus_tag="I0B46_001248"
     CDS             4701..5594
                     /locus_tag="I0B46_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAR2083560.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEKAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFYQLINPSLLRSHYSLIHPENIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            5746..7194
                     /gene="cls"
                     /locus_tag="I0B46_001249"
     CDS             5746..7194
                     /gene="cls"
                     /locus_tag="I0B46_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAR2083561.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDTLGE
                     EVREALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPEFFPTIHSTGDMAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLIYDERMASRVRKAFFEDS
                     KISTHLTKEVYENRGMMIKMKEGLARLISPLL"
     gene            7531..8388
                     /locus_tag="I0B46_001250"
     CDS             7531..8388
                     /locus_tag="I0B46_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAR2083562.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQAENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            8503..9423
                     /gene="pstC"
                     /locus_tag="I0B46_001251"
     CDS             8503..9423
                     /gene="pstC"
                     /locus_tag="I0B46_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAR2083563.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            9423..10307
                     /gene="pstA"
                     /locus_tag="I0B46_001252"
     CDS             9423..10307
                     /gene="pstA"
                     /locus_tag="I0B46_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAR2083564.1"
                     /translation="MNAKRSDKIATGVLYVVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            10322..11128
                     /gene="pstB"
                     /locus_tag="I0B46_001253"
     CDS             10322..11128
                     /gene="pstB"
                     /locus_tag="I0B46_001253"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAR2083565.1"
                     /translation="MKKYNWNETNVITMPKDKEVALYTNDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYKDVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            11144..11902
                     /locus_tag="I0B46_001254"
     CDS             11144..11902
                     /locus_tag="I0B46_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAR2083566.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWEDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            11915..12592
                     /gene="phoU"
                     /locus_tag="I0B46_001255"
     CDS             11915..12592
                     /gene="phoU"
                     /locus_tag="I0B46_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAR2083567.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            12814..13848
                     /locus_tag="I0B46_001256"
     CDS             12814..13848
                     /locus_tag="I0B46_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAR2083568.1"
                     /translation="MQKKISIIITLFFICILASWFIQRQAALYDVPVIEIERVRIEED
                     QQLITGRLMNTDQKGEVISLTAPYQKNQIETANITTRQIYLVQMHGDEWQLVAKKNDG
                     WLFFFTSVFIGTMLLIGGKQGVFALGSLIINIVLLLLLLLLFTKTEGISLLNIAILFV
                     VIGIIVSILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTR
                     PYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKQQPKISAKQLWLSGRQVGRDVIGS
                     MANILLFVYISGAIPSLLLYLGNQWSFKETVERHLSLELLRVIAGSLGIVLSIPIAVL
                     FFLAVRRLKK"
     gene            13845..14612
                     /locus_tag="I0B46_001257"
     CDS             13845..14612
                     /locus_tag="I0B46_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAR2083569.1"
                     /translation="MNVLIILVGIFAISVLFVGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILVLSIIGFLLIAVVILFFINGYNLKMRAAFASILIFLFCFLLLIPITDFLAI
                     QGFTSIELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     MSFNQLRHASFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDISFQQIINEKSFVL
                     EYTMAILTTLAALLVLPLTCVTTAYLFTKKKEHLKME"
     gene            14712..16280
                     /gene="liaX"
                     /locus_tag="I0B46_001258"
     CDS             14712..16280
                     /gene="liaX"
                     /locus_tag="I0B46_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAR2083570.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDELENGEEKISEPVDPKEKERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEATGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSDDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVNQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATPENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDSIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDDEIAQVQL
                     STTTGSIYLKDTDK"
     gene            16459..16764
                     /locus_tag="I0B46_001259"
     CDS             16459..16764
                     /locus_tag="I0B46_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAR2083571.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTKKRKEAEKIDDDEWSDF"
     gene            16764..17114
                     /locus_tag="I0B46_001260"
     CDS             16764..17114
                     /locus_tag="I0B46_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAR2083572.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALFVAILM
                     SLVNVIVSEHNSRK"
     gene            17280..18218
                     /locus_tag="I0B46_001261"
     CDS             17280..18218
                     /locus_tag="I0B46_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAR2083573.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            18233..19063
                     /locus_tag="I0B46_001262"
     CDS             18233..19063
                     /locus_tag="I0B46_001262"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAR2083574.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLVLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGSLPDYDRSLGKNQQII
                     "
     gene            19107..20132
                     /locus_tag="I0B46_001263"
     CDS             19107..20132
                     /locus_tag="I0B46_001263"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAR2083575.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHHLPDSIRAFTDMEDAVKDADAVLFVIPTKAIRSVAKELVAKLNTKPVI
                     IHASKGLEQGTHKRISEILLEEIPTEKRKAVAVLSGPSHAEEVAKHDITTITAASTNV
                     KAAKYVQELFMNHYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            20147..21085
                     /gene="galU"
                     /locus_tag="I0B46_001264"
     CDS             20147..21085
                     /gene="galU"
                     /locus_tag="I0B46_001264"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAR2083576.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            21210..21659
                     /locus_tag="I0B46_001265"
     CDS             21210..21659
                     /locus_tag="I0B46_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAR2083577.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFLKTKNILKRQEENKNG"
     gene            21652..22266
                     /locus_tag="I0B46_001266"
     CDS             21652..22266
                     /locus_tag="I0B46_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAR2083578.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQDLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(22329..23432)
                     /locus_tag="I0B46_001267"
     CDS             complement(22329..23432)
                     /locus_tag="I0B46_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAR2083579.1"
                     /translation="MNNVKINELRQWMAQHQLELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            23642..24643
                     /gene="ccpA"
                     /locus_tag="I0B46_001268"
     CDS             23642..24643
                     /gene="ccpA"
                     /locus_tag="I0B46_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAR2083580.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            24997..25392
                     /locus_tag="I0B46_001269"
     CDS             24997..25392
                     /locus_tag="I0B46_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083581.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREAQKQQDLLAVEKQS
                     VKVLKNTFADIKEVKIEQFEKNDMTGIYEALITMTNTEGKAVYFDYSFTKESNEIDGY
                     GVENEEVQKEGITVDEIIVIYTDGSEEKV"
     gene            25389..26645
                     /locus_tag="I0B46_001270"
     CDS             25389..26645
                     /locus_tag="I0B46_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HAR2083582.1"
                     /translation="MTITDKQYKGISNKVYWLDPKHPQYSKSYKEGAIKNFGETKFQI
                     LKIKNSFDGMQAMAVAPIKNGKVDISQVVIAFAGTNLSDWKDLETDARSVMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAYLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGRLTVNEQIYLDDTQARLVVEKLSVKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            26657..26980
                     /locus_tag="I0B46_001271"
     CDS             26657..26980
                     /locus_tag="I0B46_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083583.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            26973..27239
                     /locus_tag="I0B46_001272"
     CDS             26973..27239
                     /locus_tag="I0B46_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083584.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQTWAEKRLTDQNHQ
                     MTAEEATQEFSNRHAHLKESVKGQFAEKLTELFQRQEKKLKQFR"
     gene            complement(27402..29747)
                     /locus_tag="I0B46_001273"
     CDS             complement(27402..29747)
                     /locus_tag="I0B46_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAR2083585.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKDEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
     gene            30026..30928
                     /locus_tag="I0B46_001274"
     CDS             30026..30928
                     /locus_tag="I0B46_001274"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAR2083586.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            31139..32914
                     /locus_tag="I0B46_001275"
     CDS             31139..32914
                     /locus_tag="I0B46_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAR2083587.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     ILASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPTVEIIIA
                     CLLAIYYRKYNSSKKLLPWFLGLAVSFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEAKKLKERPFLLSKQEWLKLSLFLLPLVGFSIVYQWIEYKNETTYGIAAIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALDASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYQKGT
                     IKKKKEFYLMGSGDGKTMNDLPIVFDFLKSGYAYAWRYKDFHQGGNYSFGTQEQVEQA
                     QELLNLPLMDNWIDSSDPRTPDFQLTKTAKVANVMIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVIGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIAVTSMLVFSKLNLKKALSSK"
BASE COUNT        10815 a         5843 c         7066 g         9244 t
ORIGIN      
        1 ttttttgagt ttcctcctct tttagatgaa atatgaataa attatgaagt tcttacttta
       61 atacgcttaa gatatttcaa ttatctataa ctaatgataa aatgaaaagt aaagtctaca
      121 tagtctggat aatacacaga aggaaaagga gattacacaa cgcatggcca atttgattcg
      181 ttcacttatc gaaaacgata agaaagaact aaaacgttta gatagcattg caaaaaaagt
      241 tgaagcatac gctgaccaaa tggcagcatt aactgatgaa caattacagg caaaaactgc
      301 tgaattcaag ggcagatacc aaaaaggaga aacattagat caattacttc cggaagcttt
      361 tgcagtcgtc cgagaagcag caaaacgtgt attggggtta tatccatacc acgtacaatt
      421 aatgggtggg atcgtgcttc acgatgggaa tatccctgaa atgcgtactg gtgaaggtaa
      481 aaccttgact gcaacgatgc cggtgtatct gaatgcgttg actggcgaag gggtccacgt
      541 agttaccgtc aatgaatatt tagcaacccg tgactcgacg gaaatgggcg agctttataa
      601 tttcttagga ttgacagttg ggttgaatat caactcaaaa tcttcggatg aaaaacgcga
      661 ggcttataat tgcgatatca cttatagcac gaataatgaa ttaggatttg actatttgag
      721 agacaacatg gtcgtttacc gtcatcaaat ggtacaacgt ccgctgaatt atgccattgt
      781 cgatgaagtt gactcgatct tgattgatga agcgagaacg ccgctgatca tttcagggca
      841 agcagaaaaa tcaacagctt tatatacaag agcagacaac ttcgtgaaac gtttgaaaga
      901 agaagaagat tataaaatcg atatacaatc aaaaacaatc agtttaactg aagcagggat
      961 cgaaaaagct gaagaaacat tcggtttgga caacttatat gacattgaaa atactgcatt
     1021 gacccatcac ttggatcaag cattgagagc caactacatc atgcttttag acatcgatta
     1081 tgttgttcaa gatggaaaag tattgattgt tgaccaattt acgggtcgga tcatggatgg
     1141 acgccgttat tcagatggtc ttcatcaagc gatcgaagct aaagaaggcg tagagatcga
     1201 agacgaaacg aaaacgatgg cgacgatcac tttccagaac tatttccgta tgtacaagaa
     1261 acttgcgggg atgactggta cagctaagac agaagaagaa gagttcagag agatttataa
     1321 tattcaagta taccagattc cgacaaaccg tccggtcatc cgtgacgatc gaccagattt
     1381 gctttaccca acattacaaa gcaagttcca tgctgtcgta caagatatca aagatcgcta
     1441 ccgcaaagga cagcctgtct tagtcggaac tgttgcagtt gaaacatctg agttattatc
     1501 agatatgctg aatcaagaac gtattcctca tgaagtactg aatgcgaaaa atcactttaa
     1561 agaagcagaa atcatcatga atgcgggtca aaaaggtgca gtgacgattg ctaccaacat
     1621 ggctggtcgg ggaacagata tcaagctagg gcttggcgta agagaacttg gtggcctagc
     1681 agttatcggg acagaacgac atgaatctcg tcgtatcgat aaccagcttc gaggacgtgc
     1741 aggacgtcaa ggagaccctg gtatgtcaca attctacttg tctttagaag atgatttgat
     1801 gaaacgattc ggttcagaac gaatcaaagc tttcttagat cgaatgaaga tcggtgacga
     1861 agatgctgtg atccaaagca agatgctgac gaaacaagta gaagctgcac aaaaacgcgt
     1921 agaaggaaac aactacgaca cacgtaagaa cgtcttgcaa tacgatgacg tgatgcgtga
     1981 gcaacgtgaa gtaatctata aacagcgtca agaagtgatc atggaagaaa aaagcttatc
     2041 taaaccactg atgaatatgg tcaaacggac aatcagtcgt gtggtagatg cgcatacgca
     2101 attagaaaaa gaaaattgga atttggaaag tatcgtcgat tttgctggca gcacgcttgt
     2161 ccatgaagac agtatttccc ttacagatat cgaaggaaaa acctctcaag aaatcaaaga
     2221 atatctaaac gaacgagcac aagaagtatt caataccaaa gcagctcaat tgaacggacc
     2281 agagcaactg cttgaatttg aaaaagtcgt gatcttacgt gtcgtggact ctaaatggac
     2341 agatcatatt gatgcaatgg accaattacg tcagtcaatc ggcttgcgtg cctatggtca
     2401 aaacaatcca ttagtcgaat accaaacaga aggttataag atgttcgaag atatggtagg
     2461 tgccattgaa tatgaagtca ctcgtatctt catgaaagct gagatccgtc aaaacgtaca
     2521 acgtgagcaa gtttctcaag gaacagcttc tcactcagaa gatggagatg ccaaagaaaa
     2581 cagtaatact agcgcgaaaa aacaaccaat ccatgtgaac aaggttggta gaaatgatcc
     2641 ttgtccttgt ggaagcggga aaaaatataa gaactgtcac ggaaaagatg caaactaatt
     2701 tctgacagca ggattggtga gacaaattct tttttaaaga atttgtctca ccttctttct
     2761 gttatttgac gattctgtta gactagatat aataaaaaaa gtatagtgaa agaaggaaaa
     2821 aaacatggaa atatccgaaa ttcgaaatct cctgacagaa atgcaggaaa aggtcacaag
     2881 cttcaggagg tctctttgac ttagaccagc tggaagaaga cattgcggaa gcagaagcaa
     2941 aaatggctga acctggtttt tgggatgata gcgaagctgc tcaaaaagtg atcagtgaaa
     3001 acaatgcaaa taaagaaact tatgatcaat tccattctct tgctgaagaa ctggaagaaa
     3061 tcgaattgat ggtggagatg ttgcaggaag aacctgatgc agtaatgcaa gaagaagcag
     3121 aagcacgtat caatgcgcta gatgaaaaaa tgaatacgta tgaattatct atgttgttag
     3181 atggtcctta tgatcgaaac aacgccatcg tcgaattgca tccaggggct ggcggaacgg
     3241 agtcccaaga ctggggcagc atgcttttac gcatgtatac acgctgggca gagcagcatg
     3301 ggtatcaagt agagacacta gattatcaag caggcgatga agcaggaatc aagagtgtga
     3361 ccctactgat caaaggacac aacgcatttg gctatctgaa atcagaaaaa ggtgttcatc
     3421 gccttgtacg tatctcgcct tttgactcag caaaacgtcg tcatacttct ttttgttcgg
     3481 tagatgtgat gccagagctt gatgaaacga tcgatataga aatcaatcca gatgatttga
     3541 agatcgacac atatcgggcg agtggtgcag gtggtcagca catcaataag acagaatccg
     3601 ctgtacgaat cactcacttg cctacaggaa cagtagtagc gagtcaggca caacgttcac
     3661 aattgaaaaa ccgggaacaa gcgatgggga tgctgaaagc taagctttac caattagaag
     3721 tggaaaagaa agaacaagaa gcagctgcac tgagaggaga acaaaaagaa atcggctggg
     3781 gatcacagat ccgttcttat gttttccatc catattctat ggtaaaagac catcggacaa
     3841 acgtagagac aggaaatgtc caagctgtta tggacggaga tcttgattta tttattgacg
     3901 gctatttgaa aatgcatctg caataagagc cctttgttaa attgaaataa aattgtaaga
     3961 ttttgcaagc aagttgaaac attgccatgc tataatagct aaagactgaa aaagatatgg
     4021 agagaacgcc atgattgaaa tgaaggatgt aatgaagaag tactcaaacg gtaccactgc
     4081 tatccgcaat ctctcgattg tcatcgaaca aggagagttt gtgtatgttg ttggtccgtc
     4141 tggagctggt aaatctacat tcatcaaatt gatgtatcga gaagaaaaag cttcaaaagg
     4201 aactttgaac gtcgcaggtc atgatctaat agccatcaaa aaccgcgaag ttccttatct
     4261 acgcagagaa atcggcgtgg tgttccaaga ctataaatta ttaccaagaa aaacagtata
     4321 tgaaaatgtt gcgtacgcca tgcaagttat cggcagaaaa cctcgggata tcaaaaaacg
     4381 agtaatggaa gtgctggatc ttgttggttt gaaacacaaa gtccgagtct ttcctagcga
     4441 attatctggt ggggaacaac aacgtgtttc tattgcccga gcaatcgtaa atacacctaa
     4501 agtacttatc gcggatgaac cgacagggaa tctagatccg gaaaactcat gggaaatcat
     4561 gaaactatta gatcggatca atgcacaagg aacaactgtc gtgatggcta cacataacag
     4621 tacgatcgta aatacgattc gacaccgagt aattgctatc gaaaacggcc gcattattcg
     4681 agaccaagcg gaaggggaat atggatacga tgattagaac ctttttccgc catttattag
     4741 aaagtatcaa gagtttgaaa cgtaacggct ggatgacagt agcttcggtc agtgcggtaa
     4801 cgatcacgtt gactttagtt ggtgtgttca tggcggtcat catgaacgca accaaactag
     4861 cacaagatat agaaggaaac gtggatgtat ctgtgttcgt agacattggt acaaaagacg
     4921 aagagatgaa aaaattagaa aaagaattaa atgacttaga tcatgtcaaa aaagtcgaat
     4981 tttcaagcaa agaacaggaa ttgaagaaaa tccaaaacga aatgggcgat tcttggaaat
     5041 tgtttgaagg ggataataac ccgttatatg atgtatacgt agtaagtgct accgatcctt
     5101 cttatacaaa acaagtagca gaaaaagcgg cagatttgaa gaatgtttcc cgttctgatt
     5161 acggtggggc ttcctcagat cggattttcc aaattgctgg tgcagttcga acatggggat
     5221 tagttgcggc ggcattgctg ctgtttgtag caatgtttct catttctaac acgattcgga
     5281 tcacgatttt atctagacag cgtgaaattc aaatcatgcg tttagtaggg gcaaaaaatg
     5341 gatatatccg ctggccattt ttccttgaag gtggttggat cggcttaatc ggtgccattc
     5401 ttccgatact gatcattaca tttggttacg catggtttta ccaattgatc aatccgtcat
     5461 tattacgatc tcattattca ttgatccatc cagaaaatat cgtttggaaa atcaatcttt
     5521 tgatggtcgg tattggtgtg atcattggtt cactcggctc gatcatctct atgagacgtt
     5581 tcttgaaatt ctagtgtcac aagataaata taatcagaaa gttggacgat ttcgttccag
     5641 ctttcttttt ttatcagcta ggtggagtaa atggggcggc ttttttctct ttactttaaa
     5701 aaacaacttg ggtattctta agttggtaga aaaagaggtg aagccgtgaa gattatcgtt
     5761 gacaattttt ttacgatcct tttgattatg aatattttgc tttcctttat tatcgttttt
     5821 cgagaaagaa aggaaactgc gcaaacatgg gcatggctgc tcgttttgat gttcattcca
     5881 gttgtaggat ttattttata tattttttta ggaagaggga tttccaaaga gaaaatcttt
     5941 gatttgaaga tccaagggaa aatcggtaag aatctagaaa tcgaagaaga taaacaagca
     6001 ttgatgcgag ggctttatcc tcatccgcct actggacaag tagatgtgaa acaattgatc
     6061 tatatgctta ctgtatttga aagtacgctt tatacgacta aaaatgaaat cactttattt
     6121 acagatggtc gtgaaaaatt cgatgcgctg atcgaggata tcaaacaggc agaagaccat
     6181 atccatttcc aatattatat ttaccgcagt gatacattag gtgaagaagt tcgagaggca
     6241 ttgacagagg cagcaagacg cggagtaaaa gtcagagtcc ttcttgatgc atggggttcc
     6301 acacaagttt cttctagttt ttttcagaat ttgaaaaaag caggtggaga agtcgcattt
     6361 ttctttcctc tattcgttcc gtatatcaat cctcgaatca attatcgcaa tcatcgtaaa
     6421 atcgtagtga ttgatgggaa aatcggatat acaggtgggt tcaatgttgg aaacgaatat
     6481 ctaggattgg taaagaaatt tggttattgg cgagacaatc atctgcgtat ctatggagaa
     6541 gcggtttaca ccttgcaaaa ccgttttttg atggactgga attctcagca tacaaaagaa
     6601 gtaggatatt caccggaatt tttcccgacc attcattcta caggtgatat ggctgttcaa
     6661 atcgtgacaa gcggaccaga taccgaacat gagcaaatca aaatgacata tttgaaaatg
     6721 atttcgttag ccaaacggga gatattgatc caaacgccgt attacatacc ggatggatcg
     6781 attcatgagg cattgaagtt ggctcttctc tcaggcgtaa aagtccatat ccaaatacct
     6841 aacaaaccag atcatcttct cgtttactgg gcaacgtatt ctttcgctgc agagctgatc
     6901 gagtatgggg caagaatcga aacatatgaa aatggattta tccatgcaaa gacgatgatt
     6961 atcgatggcg gcatcgtttc cgttggttca gctaatatcg acgttcgctc atttagattg
     7021 gattttgaag tcaatacact gatctatgat gaacgaatgg cttctcgcgt aagaaaagca
     7081 ttctttgaag attcaaagat ttcgacgcat ctcacaaaag aagtgtatga aaaccgaggg
     7141 atgatgatca agatgaaaga aggattagca agactgattt cgccgctcct ttaaaataca
     7201 aaaaggacag gagagccatc aatagatgga aatctcgcgt ccttttttct cggggccatt
     7261 tctgagagta cgtcagaaat ggagaaagcg tctcgcatga gcgaaattaa ctttggggta
     7321 tctcttgttt tttggggaaa gcaagaaata agctaatata tgctattaaa agagacacat
     7381 ttcaaagcca gtatagcata gaaactttta atgagatagc tttttttgca attatgagaa
     7441 tttattttgt tatttaatga ttttgattcg ttttcatcaa gtaatgaaaa tgatagaata
     7501 aacatacttt aggattaaag gagatttcta atgaaaaaac taaaattatt cttaccaata
     7561 ataggattag ctattctttt atcaggctgt tcaaagtgga ttgatcgagg agaatccatt
     7621 actgcagttg gctcttctgc cttacagcct ttagtagaaa cggttgctga aacctaccag
     7681 gcagaaaatc ctggaaaatt catcaatgta caagggggcg gaagcggaac tgggttgtct
     7741 caagtacagt caggagcagt cgatatcgga aattctgatt tgttcgcgga agaaaaatca
     7801 gggattgatg cttcggcatt agtcgatcat cgagtagcag ttgttggaat cactcctatt
     7861 gtgaacaagg atattggcgt aaaagatatc agtatggaaa acctgaaaaa attatttacg
     7921 ggcgagatca aaaactggag tgaagtcggt gggaaagatc agccagtagt tatattgaac
     7981 cgtgcatcag gaagcgggac ccgtagtacg tttgaaaaat gggtattgga cggagaaaca
     8041 gctgttcagg cacaagaaca agattcaagc ggaatggtgc gccaaatcgt tgcggacaca
     8101 cccggagcaa tcagctatgt tgctttttct tacgtttccg atgatgtgga tacgttgaac
     8161 gttaacggtg tggaaccaac agatgaaaat gtgacaacga acaaatggac gatttgggca
     8221 tatgagcata tgtacacaag agaccatcca aaaaaactga caaaagaatt tttagactat
     8281 atcttatctg atgaagtaca agataatatt gtcggagagc taggctatat ccctgtttca
     8341 catatgaaag ttgaacgtga ttggcaagga aacattgtta aagagtaatc tttacacaaa
     8401 atttacaaac ttagctaaac ttttacatgt tattaaaacc aaattgatgt tcaatttgta
     8461 cactgagtga gtatgttaaa cctgattaag ggggagtcat ctgtggaaga tgtgaaaaaa
     8521 gtattattaa caaaatcaaa acgttcaaaa atggaacagc gcggaaaatt cattagtttt
     8581 ttgtgtatcg cgctgatcgt gttggttgta ttatctattt tttattttgt cgctagtaaa
     8641 ggattggcca ccttttttac tgataagatc aatgtatttg attttctgtt tggtacagat
     8701 tggaatccaa gccaaatagg agcaaatggg aaacccatgg taggggcatt gcctatgatc
     8761 acaggttcat ttattgtgac ctttttatct gctattgttg ccacaccatt tgctattgga
     8821 gcagcagtgt tcatgacgga gatttctcct aaaaacggaa cgaagatctt gcaaccggtc
     8881 atcgagctac ttgtaggtat tccgtctgtc gtttacggat ttatcggttt atctgttatc
     8941 gtgccttttg tcagaagtat ctttggcggg acagggttcg gtatcttagc tggtacattt
     9001 gtgttattcg ttatgatctt accgactgtc acaacgatga ctgtggatgc attaaaggca
     9061 gtgccgcgac actatcggga agcttcatta gcgctaggtg ccacaagatg gcaaacaatc
     9121 tacaaagtgg tgctgcgagc agcagtgccc ggaattttaa cagcagtcgt ttttggtatg
     9181 gctcgtgcgt ttggcgaagc cttagctatc caaatggtta ttggtaatgc ggcattaatg
     9241 ccaacaggac ttactactcc tgcttctaca ttgacgtcca tcttgacaat gggaatcgga
     9301 aatacaatca tgggaacagt tgaaaataat gtgttatggt cattagcgct gatcttgcta
     9361 ttgatgtcat tattctttaa tatcgtgatc cgtatgattg ggaagaaagg agagctgaaa
     9421 taatgaacgc aaaacgctca gataaaattg ctacaggtgt tctatatgtg gtttcaggga
     9481 tcatcgtatt gatcttagca gctttattac tttatatctt agttcgagga ataccgcaca
     9541 tttcatggtc attcttgact gaaccttcca aagcctacca agaaggcggt gggatcggga
     9601 ttcaattatt taactcgatt tatttgcttt tgattaccat gatcatcagt tttcctatct
     9661 ctttaggtgc gggtatctac ctttcggaat atgccggaaa aaactggctg acagacgtga
     9721 tccgtacgtc tatcgaaatc ttgagctctt tgccttctgt tgttgtcggt ttattcggtt
     9781 tcttggtttt cgttattcaa ttccagtatg gtttctcaat catttctggt gcattagctt
     9841 tgaccgtatt caacttgcct cttttgactc gaacggtcga ggaatcttta caagcggtcc
     9901 attacacaca acgagaagct ggattggcgt taggtttgtc acgctgggaa acagtgataa
     9961 aagtggttgt tccagaagcg acaccaggaa ttttgactgg tgtgatccta agttcaggaa
    10021 gaatttttgg tgaagctgcc gcattgattt atacagctgg tcaaagcgcg ccagcattag
    10081 attttacgaa ctggaatcca ttaagtgttt ctagtccgat cagcattttc cgccaagctg
    10141 aaacactggc tgtccatatt tggaaaatca acacagaagg tacgatgcct gatggtgttt
    10201 ctgtttcggc tggcgcttct gccgtgttga tcattgctgt tttactgttc aacttcggcg
    10261 ctcgtgcgat tggcaaacga ttataccgaa aaatgacttc agcctaagga gaagaagtaa
    10321 aatgaaaaaa tataattgga atgaaacaaa tgtcattacc atgccaaaag ataaggaagt
    10381 tgcattatat acgaatgatc tgcatgtttg gtatggcgac aatgaagcaa tcaagggagt
    10441 ggatctagaa ttcgaaaaaa ataagatcac tgcattgatt ggtccctctg gctgcggaaa
    10501 atccacgtat cttcgttctt tgaatcgtat gaatgatggg attgctaata cgaaaattac
    10561 tggaaagatt atgtataaag acgtggatat caatgcgcct caagtggatg tttatgaaat
    10621 gcgtaaacgg atcggcatgg tgttccaacg ccccaaccct tttagcaaat cgatttatga
    10681 caatataact tttgctttga aacagcatgg tgaaaaaaac aaaaagaaat tagatgaaat
    10741 cgttgaaaca agcttgaaac aagcagcttt gtgggatcaa gtaaaagata gcttggataa
    10801 aagtgccttg gctttatctg gcggacaaca gcaacgtttg tgtatcgctc gtgcaatcgc
    10861 gatgaaaccc gatatcttgc tgttagacga gccggcaagt gccttggatc ctatttcgac
    10921 aggaacggtt gaagaaacac ttgtaaactt gaaagaagac tacacgatca ttatcgtgac
    10981 gcataatatg caacaagcag ctcgtatcag cgactatact gcctttttct atctaggaaa
    11041 agtaatggaa tatgacaaaa caaacaagat atttaccaga ccaaaaattc aggcaaccga
    11101 agattatgtg tccggtcatt ttggttaaga cggaggcctc aacatgacaa aagaaattat
    11161 ctcatccaaa gatcttcatc tgtattatgg aaagaaagaa gcactaaagg gaattgatct
    11221 aagtttcaat caaggtgaaa tcacagcgat gatcggtcct tctggttgcg ggaaatctac
    11281 ctatctacgt tcgttgaatc gaatgaacga tctgattcca aatgtaacga tcaccggaag
    11341 tgttttatat aaagacaaag acatctatgg tccgaaaaca gatgttgttg aactaagaaa
    11401 agaaatcggc atggtgttcc aacagccaaa tccatttcct ttttctattt atgaaaatgt
    11461 catctatgga ttgaagttaa aaggtgaaaa agataaaaaa gtccttgatc aagtagtgga
    11521 agaaagtctg aaggcagcgt ccgtttggga ggatgtgaaa gataaactgc ataaaagtgc
    11581 cttgtcttta tccggtgggc agcagcagcg tgtatgtatc gcacgtgttt tggcagtgaa
    11641 tcccgaaatc atcttattgg acgaaccaac aagtgcactt gaccctgttt ctactgggaa
    11701 aatcgaaagt atgctgttag aattaagaga gcggtatacg atgatcatcg tgacccataa
    11761 tatgtctcaa gcttcacgta tttcagataa gacggcattc tttttagatg gtcacttgat
    11821 cgaatacaac gatacgaaga agatattcat gaatccagaa aaacaagaga cagaagatta
    11881 tatttcaggt cgatttggat aaggaggaaa cggaatgcta cgtacacaat ttgaagaaga
    11941 attgctgaat ctccataatc agttctacga aatggggatg atggtgagca gcgctgtgca
    12001 caagtcagtt cgtgcataca tcaaacacga taaaatctta gctcaagaag ttatcgaaaa
    12061 cgatataaat atcaataata tggaaacgcg tttggagaag aaaagttttg aaatgattgc
    12121 attacaacag ccagttacaa ctgacttgcg tatgattatc acggtcatga aagctagctc
    12181 tgatttagaa cgtatggccg atcatgcggt gtccgttgct aaatccacaa tccgcgtaaa
    12241 aggacagacg agaataccgg aaatcgaaaa agagatctcc gatatgtccg attacgtgaa
    12301 aaaaatggtg gacaacgtcc tagttgcgta tgtcaaaaca gacgaaaaag atgcgcgtac
    12361 aatcgccaat atggatcaaa gagtcaatga ctatttcaat cgaatctata atgcaaccat
    12421 caaaaacatg caagaaaatc ctgaaacagt catcagtggc acagactatt tgaatgttgc
    12481 cagctatcta gaacgaatcg gagactatgt gacaaacatc tgtgaatgga tcgtctactt
    12541 ggctacaggg aaaatcactg aattaaatac gaaccacaat gagattttct aaactgaagt
    12601 tgtgattctg ctgtttattt aggaaaagag gttgtgaaat cagcgttttg tcctgagtat
    12661 tagagaaaca aacggtaaaa tagttcctca tattttgccg ttttttgagc taataccgaa
    12721 ggtcagctga ttgaacaccg tttattctgg aaaagggttt gtgacaaaac atgtcacaaa
    12781 cccttttcat tattccaata gaaaggataa agaatgcaaa aaaagattag catcatcatc
    12841 actctatttt ttatttgtat acttgcttca tggtttattc agcgtcaagc tgctctatat
    12901 gacgtacctg tcatcgagat cgaaagagtg agaatagaag aggatcaaca attgattaca
    12961 ggaagattga tgaatacgga ccaaaaagga gaagtgattt ctttaacggc tccttatcaa
    13021 aaaaatcaaa tcgaaacagc aaatataaca acaagacaaa tctatcttgt ccaaatgcat
    13081 ggagatgaat ggcaactagt agcaaaaaaa aatgatggct ggcttttttt ctttacaagt
    13141 gtcttcattg gcaccatgtt gcttattggt ggaaaacagg gcgtatttgc tttaggaagt
    13201 ctcatcatca acatcgtttt acttttactt ttgttgctcc tctttaccaa aacagaaggt
    13261 atttctctct taaatatagc aatcttattt gtcgttatag ggataatcgt tagtatattg
    13321 gcgttagacg gatggaatag aagcagtata atcaaaattt cggctgcagt tacaagtgtt
    13381 tttttagcat tttttatttg tcttttgaca atgaatcatt ggaaagatca aggactgcgc
    13441 tatgaagaac tgaattatct gacacgaccg tatcgttctg tttttctttc cagcgtgctg
    13501 attgggacag ctggagggac actcgatacc gtgatcacag tcattgcgac attagaagaa
    13561 ttgaccaagc aacaaccgaa aatatcagcg aaacagttat ggctttcagg aaggcaagtg
    13621 ggccgagatg tgattggttc gatggctaat atccttttat tcgtctatat cagcggagcg
    13681 attccatcgc ttctgttata tttaggaaat caatggtctt tcaaagaaac ggtagaacga
    13741 catttatcat tagaactttt acgcgtcatt gctggcagct taggaatagt attatcaatc
    13801 cccatcgcgg tcctcttttt tctcgctgta aggaggttaa aaaaatgaat gtacttatta
    13861 tacttgttgg gatttttgca atcagtgtgc tatttgtcgg tggtacgcaa ggtatgtata
    13921 ttcttttagg attattcatt aatttaggaa tctttttcct attattattc ggctatcatc
    13981 agaagtggcc gatattggta ttgtcgatta tagggttcct attgatcgct gtggttatct
    14041 tattttttat caatggttat aatctgaaaa tgcgagccgc ttttgccagt atactgatct
    14101 ttttgttttg tttcctacta ttgattccta tcactgattt tttagcgatc caaggcttta
    14161 cttcgataga actagaagaa ctatctggct tagataagac gttagcaatt gattttagac
    14221 tacttacgag gtcgttgctt ctgatcagtc ttagcggagc tgttttagat gcgagtgtag
    14281 cgatttctag tggaacattc gaagtatatc aggcaaatcc gcaaatgtct tttaaccaat
    14341 tgcgccatgc tagctttgcc attgctaaaa aaatattggc atcaacagtg atgacgcttc
    14401 tttttgcgtt tatggggagt tctttagcgt tgattctttg gtttattgac ttagacattt
    14461 cttttcagca aataatcaat gaaaaatcat ttgtcttgga atatacgatg gctattctta
    14521 ccacactggc cgcactactt gtcctgccct taacttgcgt gactacagca tatctcttta
    14581 caaaaaagaa agaacatctc aagatggagt aaaagttaca acttaagtcc tatgacattt
    14641 ttactgaatc agtgcataat atggatgtaa ggaaaagctt gcaaacaatt ttgcttaagg
    14701 aggcaatttc catgaaagaa agagaacgta ttttagactt agtgaaaaaa ggaatccttt
    14761 cgactgaaga aggcttagat ttattagaaa gcatggcaac tgaaaaagac gaaaaacaaa
    14821 tcaaaaaaga agcagacaag gtaactgcta gtcataaaga aaaagatcag gcaagtcaat
    14881 tgatcgatga acttgaaaac ggtgaagaaa aaatttctga gccagttgat ccaaaagaaa
    14941 aagaaagaca agatcaagaa aacttagaaa agatcttaga tgaactagca actgaagcga
    15001 ataaaacttc ggcgcgtcta gacgaagtaa atgctcaatt cgctgacaat aaggcagcac
    15061 gtaatgagaa acaggaagca ttgatgcagc tgaatacaaa agaagaatta ggcgagttga
    15121 cagaagaaga attagcacag cgtcaaacgt tagaagcaga aatcaaagag ttagaagcaa
    15181 ccggcgatac actattagaa gaacaattga agctggaagc agaattaaaa gatatccgca
    15241 agaatcaatg gtctgagaaa aaagaaacat ttacggataa gtttgaattg ccagatgatt
    15301 ggaaagatca agcgacagat acgctgaatc aagtaggcga aaaaatgtca gaagctggat
    15361 cacagttagg caaattcttg aagaaaacat tccaaactgt ttcagaaaca gtaaatgata
    15421 acatggaatg gaaagatgtc agcttacgcg tccctggaat tgcgacaacg aaatttgaac
    15481 acgaatttta ttatgaagct cctgcagcaa gtatccttga tatcaaggca gcaaacggaa
    15541 acgtgacgtt aaaaacgtgg gacagtgatg atgtgaaagt ggaagccaag atcaaacttt
    15601 acggaaaaat gggtgctgaa ccatttgaag cattctctga acggagccag atcgaggtca
    15661 atgaagatca tatctccttc caaattccaa ataagcgtgt gcgtgcagac cttgttttct
    15721 acttgcctaa acgagtatac gaccatgcag ctatcaaact tctaaatggc aacatcatga
    15781 ttgagacatt ggaagctaaa gatatttata caaaatctac gaatgggaac atcatcgtga
    15841 accaattaac tgcaacgatg ctggaagtag aaggtgtaaa cggcaatatc gatattcgaa
    15901 acgggaacat cttagattct attatagaaa cagtaaacgg tacagtgaca tttggtgcca
    15961 caccagaaaa cctttctgtg tctttagtaa atggtgatgt acgtcttacg attaaagaag
    16021 ataatttgaa aaaagtagaa gcaagttcag tgaacgggaa tgtcaaagtt gctttaccag
    16081 atagcatcgg tttggaagga cacgctaaaa caagtcttgg aagcattaac agccgtctgt
    16141 ccaattacga agtagttcgt gagaaaaaag aacggacaaa ccaaatgttg caattccgtc
    16201 gtgtttcaga tgatgagatc gcacaagtgc agctttcaac gacaacagga agtatctatt
    16261 taaaagatac agataaataa agaggttgtg aaagagatgt tcagccttga gcaataagaa
    16321 gcgatttttt aaaatagctt cccctatttt aaaaaaatgt gccttattgc cgataggcta
    16381 tctcttgaac accgattaag aagaaggttg tcacaacgat tttcttcatt taaaatagta
    16441 gaacagaggt gagacatgat gaaaaaaaag ttgacaaaat ccaataaaaa tgttgttttg
    16501 acaggtactt tagcagggat tggggaatat atcgggattg atcccactgt tatccgtgtg
    16561 ctttatgtat tcttaagctt agttgctttt ggaagtccaa tcattttata cattttatta
    16621 gctttgatta tcccatctgc gcgttctgca cgaacaaact atggacataa caatgcatat
    16681 tatgaaaaaa acaattatcg tgaacaaaca aaaaaacgca aagaagcgga aaaaatcgat
    16741 gatgacgaat ggagtgactt ttaatgtcgt actttcagcg tattttggtt aatacgttaa
    16801 cattcatatc gttggctgtt ctttttcctg cgatggtcca tgtgagcagt atttggacgg
    16861 ccgtccttgc cagcttcgta ttatctgtgt tgaacatgtt tgtcaaaccg attttgacga
    16921 tcctgtccct gccctttaca ttagtcacat tgggattgtt tacgtttgtt atcaatgcag
    16981 cgattcttaa aatgacttcc ttttttgtag gagcagaaca ttttagtttt tcttcatttg
    17041 gtgcagcgtt attcgtagct atcttgatgt cacttgtcaa tgtaattgtt agtgaacata
    17101 acagcagaaa ataaaaaaag ctgtgccgag atctcggcgc agcttttttg tttagaataa
    17161 agaatgcgtt ttctattaca ttggtaatta aataaaaacg ttttttttaa ttggtagtac
    17221 ttttggctag acaatgataa aatgatagag aacactctaa tagaaatgag gtagcatgaa
    17281 tgtcagaagt tgtaaaagtt agccaattag taaaaaaatt atctttggaa atcgtcacag
    17341 gcgacgagaa aagtttagac cgcgtcatcg tgactgggga catctcacgc ccaggactag
    17401 agttgaccgg ttattttaat tattattccc ataatcgttt acagctattt ggcagtaaag
    17461 aaatcagttt tgcggaacgg atgatcccag aagagcgttt gatcattatg agaagattat
    17521 gtagtgaaga tatgcccgca tttattattt caagaggatt gccggctccg gaagaaatga
    17581 tccaagcagc caatgaaaat gggatagctg tgctgcgttc gcctatctca acgtcccggc
    17641 tgctaggtga attatctagt tatttagatg gacgcttagc ccctcgaaca agtgtccacg
    17701 gtgtgttagt cgatgtctac ggattaggtg tactaattca aggggacagt gggataggga
    17761 aaagtgaaac ggcgttggaa ctgattaagc gcggacatcg attgatcgca gacgatcgag
    17821 tagatgtcta tcagcaagat gaactgacgg taatcggaga acctcctaaa atcctggaac
    17881 atctgattga gattcgaggg atcggtatca tagatgtaat gaatcttttc ggtgcgagtg
    17941 cggtacgagg ctctatgcaa gtccagttag ctgtttatct agaagcatgg gcgaaagata
    18001 agaaatacga ccgtctgggc tctgaagaca cgaccgttga aatcgcggaa gtgggcattc
    18061 cacaaataaa aatccctgtc aaaacaggac gaaacgttgc aatcatcatt gaagtagccg
    18121 caatgaattt ccgcgcacgt acaatgggct ttgacgcaac gaaaacattt gaagaaagat
    18181 taagtcgctt gattgaagaa aactcaggca atgactagca aggagtgaca atatgttagc
    18241 acaaatcaat cgagttgctt ttgagcttgt aggaattcct atctactggt atgcattgat
    18301 tatcgtatca ggaatcatta ttgccatgtg gctcagcagc agagaagctg ttcgggtagg
    18361 attaaaagca gatgacgtga cagattttat gttgtgggga ttgccgattt ccattattgg
    18421 tgcccgcttg tattatgttt tattcgatct tccccaatat atagctgatc caatccagat
    18481 attcaatatc cgttcaggag gattagcgat ttacggggga ttgattgcag gtgcaatcac
    18541 actgtacttt tttaccaaat atcattttat ttctttgtgg acctttttag atatcgctgc
    18601 accaagcgta ttattggcgc aggcaatcgg acgatgggga aatttcatga accacgaagc
    18661 gtatggtcca gacacgacac gaagtttctt agaaagtctg catttaccgc aattcatcat
    18721 tgataacatg tatatcgaag gaatctatcg acaaccaacc tttctttatg aatcactttg
    18781 gagtttgatt ggatttattc tgcttttggt tttgcggaag aaaccacatc ttttgaaaaa
    18841 aggagaagtc cttttatgtt atctaatgtg gtatagtttc ggacgtttct ttatcgaagg
    18901 gttacgcaca gacagccttt actttttagg tgtgatccgc atttctcaat tattgtctgc
    18961 tgttttattt gtcggcacat tgattctgtt tatttggcgt agaaaaaaag gaagtttacc
    19021 ggattatgac cgctcattag gaaaaaatca acaaattatt tgattttcag agcgttaaaa
    19081 atgaaaaaaa tttcaggagt ggtaaaatga attatagagt agcggtttta ggaccaggct
    19141 cttgggggac tgctttagca caagtcctcg ctgaaaacgg acatgacgtt cggatttggg
    19201 gacatcgtgc ttcacaagta gaagaaatca acacccaaca caccaatcaa cgttatttac
    19261 cagatcatca tctacccgat tccatccgag cattcaccga tatggaagat gcagtaaaag
    19321 atgcagatgc ggtactattc gttatcccga cgaaagcgat acgttctgtt gcaaaagaac
    19381 tagttgccaa attgaatacg aagccagtga tcatccacgc aagtaaggga ttagaacagg
    19441 gaacgcataa aagaatctct gaaatcttgt tagaggaaat cccgacagaa aaaagaaaag
    19501 ctgttgctgt tctttctggg ccaagccatg cggaagaagt agcaaagcat gacattacga
    19561 cgatcacagc ggcaagcaca aatgtaaaag cagcaaaata tgtacaagag ctgttcatga
    19621 accattattt tcgaatctat accaaccctg atgtgattgg tgtagagatg ggagcagctt
    19681 tgaaaaatat tattgccatt ggagctggtg ctattcacgg cttagggttt ggcgataatg
    19741 caaaagcggc aatcatgacg cgcggattag ctgagatcag ccgtttaggt gtagcaatgg
    19801 gtgccaaccc gctgaccttt attggcttaa gcggagtagg tgatctagtc gttacttgca
    19861 caagtgttca ttccagaaac tggcgcgcag gcaacttgtt aggacaaggt cagcctctag
    19921 aagaggtgct agccaatatg ggaatggttg ttgaaggtgt gaatactact caagcagcga
    19981 tggaactagc ccaaatgatg gaaatcgaaa tgcccatcac tcaaacaatc tatgaagtgc
    20041 tgtatgaaca aaaagaaatc aaaacagccg caaaagaaat catgttacgt gatgggaaaa
    20101 aagaaaatga attctcatta acaatttttt gaaggagcga atgtatatga aagtaaaaaa
    20161 agcagttatt ccagcagcag gattaggaac aagattttta ccagcaacaa aggcgatggc
    20221 aaaggaaatg ttgccaatcg tggacaaacc aacgatccaa ttcatcgttg aagaagcgtt
    20281 gaaatcagga atcgaagaca tcttgatcgt tacaggaaaa gctaaacgac caatcgaaga
    20341 tcactttgat tcaaatatcg aattagaaac aaacctaagt gaaaaaggaa aaaccgaatt
    20401 attgaaatta gtagaagaaa cgacagatgt caatttgcat ttcatccgtc aatctcatcc
    20461 aaaggggtta ggacacgccg ttcttcaagc aagagccttt attggaaatg aaccgtttgt
    20521 cgttatgcta ggcgatgata tcatggaaga cgaagtacca ttgacacagc aattgatcga
    20581 tgactatgag cgtacacacg cttctacgat tgccgttatg caagttcctc acgatgaaac
    20641 ttcaaaatac ggaatcatca acccagggga agtactggaa gatgggttat acaatgtcaa
    20701 aaactttgtt gaaaaaccag atccaagcaa agcaccaagt gatttagcta ttattgggcg
    20761 ttacttattg actccagaaa tctttgatgt attatcagaa caacaaccag gcgctggaaa
    20821 tgagatccaa ctaactgacg caatcgatac gttgaataaa acacaacgcg tatttgcccg
    20881 taaattcaca ggtaaacgtt atgacgttgg tgacaaattt ggttttatga aaacaagtat
    20941 tgaatatggt ctgacacatc cagaagtagg ggcacctttg cgtgaataca tcatgtcttt
    21001 aggtaaaaca ttagaagcag aaggaagcaa gatcaatcat aatccaaaag gaataacaga
    21061 aatcaaagaa gaaaagaaaa aataatcttt ttttctaacg agcttcagct gctaattgaa
    21121 taaaaagtag ttgaagctcg tttttatttg tttaaaaaac acgaaaatgc tatccttata
    21181 gaaaagagag acaagaaggg agtttgatca tgtcaggagg agaaattgca ggattgatcg
    21241 cagcaatcgc ctttgcgata cttgtcgttt tcatcgttcg cgtattgttg caagtttcca
    21301 aaactttaga aaaagtagac cagactgtcg cagaagcaaa tacgacaatt gagatcgtca
    21361 cacaagacgt agatctttta tctagacaag tagaaggctt gctagtcaaa agtaatgagc
    21421 tgctcttgga tatcaatgga aaagtcgcaa cgatcgatcc attgttcaca gcagttgctg
    21481 atttaagtga aagtgtatcg gaacttaatc attctagtaa aaacatcgtg acgaaagtcg
    21541 gcggaatcgg aaaaacgaca gctaaagcga ctgttgccgg aaaagtcggt ggaacagcga
    21601 tgaaattttt aaaaacaaaa aacatactga aacgacagga ggaaaataag aatggctaaa
    21661 aaaggtggat ttttattagg tgcagtagtt ggaggtacag ccgcagcaat cgccgcatta
    21721 ttgttagcac ccaaatctgg taaagaactt cgggatgaat tggctgctca agcagatgat
    21781 ttgatggaca tggcttcaga ttacacagat atggcaaaag aaaaatcatc tgagttaaca
    21841 ggtattgcaa aagaaaaagc gcaagatctt tctcaacaag caggggatct agcaggcaat
    21901 gttaagcaga aggtaagtga atcagcagag gaagcaggaa gcgaatcaga tgatatcttg
    21961 ttaaacctca aagaacaatc agctgattta tctaatcgtt tcaaaaaaac agcagaagat
    22021 ctgaaaaagc cagcagacga attaggtagt attgctaaag atacttcaga agatatcatg
    22081 atcgatgtaa aagatgctgc agataaagca ggagagacgg tcaatgaagg aatgtcagaa
    22141 gcaaaaccag ttgcagatga cacaaaagaa atgttgaaaa tggcaaaaga agatgcaaaa
    22201 gaagatgcaa aagaagctaa aaaagaaatg aaagatacaa tgacagattc gactaccaat
    22261 cactaagatt actcatttct agaataaaaa aggtgagagc ggaccagctc tcaccttttt
    22321 tattttcatt actctaaaac aagtaattct tttggtgtag tcgtgaatgg cacacaacca
    22381 tcacttgtca catacacaca atcttcgata cgtaccccta ctttttcagg aatatagatt
    22441 cctggttcaa gagagaagca catcccttct tcaatcacta agtcgtttcc ttctacaaga
    22501 gaagggaatt cgtggacggt tgttccgatt ccgtgaccta aacggtgatt gaaatattcg
    22561 ccgtagccat agctgttgat cacgtttcgt gcgatttgat caagttcacc tgctgtcaca
    22621 cctgggcgaa ctgcctcttg tgcggcaagc tgtgcttcaa gagtgatgtt atagattttt
    22681 tcttggaatt cagttggttt ttgataagca actgtacgag tagcatcact gcaatagcca
    22741 ttccaaataa cgccaagatc gaataacact agttcatttg gtgaaacctt cgtgcttcct
    22801 ggtgtaccat gagggcttgc agcatttgct cctgcaagga ctaatgtgtc gaaagacatt
    22861 tgtgaaacac cttgtttttt taattggtat tcgatttctg cgataattgc ttgttcagtt
    22921 acgccttctt ttactgtttt gaagccaatt tcaaaggcaa catcggccca tttaccagct
    22981 tcgatcagtt tgtctttttc ttctaccgtt ttgatcagtt gcagacgctg gataatagaa
    23041 gttagatcgg tgtaagaatc tgtacctgtc aagatatcag agatagcatc gaagcgatcg
    23101 acagtcagag cattcttttc aatacctaca ttaccaggtt gcccatagcg tgcgcgaagc
    23161 tcttttccga tgatctccca aggattttcg ctgtctaagt agccacgtac atcgtacgcc
    23221 cagctgctgt tttgagcatc ttcaacttcc agagcaggag tgaaaaggaa aggatcttgt
    23281 tccagaggga taaaaagagc tagtacacgc tcgtgaggat cactttcata tccggaaaag
    23341 taagcgatgt ggccagggtc gctgatataa gccaattcta attggtgttg tgccatccat
    23401 tgacgtagtt cattgatttt tacgttattc atttggcaac ctactttcta aattaatttt
    23461 gtattcaata attgtaccac tgattgatta aaagaccaac gttcttaaaa ctgaaaaaag
    23521 taaaaatttt catgaaaaca gagataaaac ggttgcaatt gttgagatta tttgatatcc
    23581 tattttagaa atgtaagacg tattttcaga aaacatataa aatatgaata ggagaacaca
    23641 aatggaaaag caaacaatca ctatctatga tgtagcgaga gaggcaaatg tttcgatggc
    23701 tactgtttca cgtgtcgtaa atggcaatcc aaacgtcaaa ccagcaacaa gaaaaaaagt
    23761 cttggaagtt atcgatcgct tggattatcg tccaaatgca gtcgcacgtg gcttggcaag
    23821 taagaaaaca acaacagttg gggtaatcat tcctgatgtc agcaatatgt tctttgcctc
    23881 tttagcacgt ggtatcgacg atgtggcaac aatgtacaaa tacaatatca tcttagcaaa
    23941 ctcagatggc aacgatcaaa aagaagtcaa tgtattgaat aatttattgg ctaagcaagt
    24001 ggacggcgtg atctttatgg gtcaccatat cacagacgaa atccgtggag aattttcacg
    24061 ttcaaaaacg cctgttgtac tagctggttc cattgatcca gatgaacaag taggtagcgt
    24121 aaacattgat tatgtatcag caacaaaaga tgcagtaaat aaattagcaa aaagcggcaa
    24181 taaaaaaatc gcttttgtca gcggtgcgct gattgatcct atcaatggtc aaaaccgtct
    24241 aaaaggctat aaagaagcat taagcgaaaa caatctacca tatagcgaag gattgatttt
    24301 tgaggcacaa tacaacttta aagatggtct gtctctagtt gaccggatcc ataatagcgg
    24361 tgcaacagct gtctatgttt cagacgatga attagccatt ggtatcttgg atggtttatt
    24421 agaccgcggc gtaaaagtac cagaagaatt cgaaatcatc acaagcaaca attctttact
    24481 gacagaagta gctcgtcctc gtttgacaag tatcacacaa ccattatacg atttaggagc
    24541 ggtatccatg cgcctattga cgaaaatgat gaataaagaa gaagtcgaag aaaaaacgat
    24601 tatcttgcct tatggaatcg aagaaaaagg atcaactaaa taataaaaga tcaatcattt
    24661 aaaggttgtt cccaaagtgg tcaacttctg gaacaacctt tatacgagaa aaggggttat
    24721 gacaaaattt tgtcataacc cctttttttg tgactttaaa aaaaagagag aatggcggaa
    24781 acaaacctta cgtccatcgt atacgacaca atctgtgcgt agatgctaca ttttgttcac
    24841 tactatcaaa attactttaa aatgaaaacg tgataaaaaa gaaagtagaa aagtagaaaa
    24901 gtaactctaa aaaatccaaa aaaatccagg ctcgattgcg tacttatctt aaaggagaaa
    24961 caatccatca tacataaccc gaaaggagac ttctatttgc ctaagaaaag acgaaagacc
    25021 atagtcatca ttttgaccat tcttactata ggaatcggag gaaaatttta catggacaaa
    25081 agagaagcgc aaaaacagca agatttactc gcagtagaaa aacagtccgt caaagttctg
    25141 aaaaatacct ttgcggatat caaagaagtg aaaatcgaac aatttgagaa aaacgacatg
    25201 acaggaatct atgaagcatt gataacgatg accaatacag aaggaaaagc cgtttatttt
    25261 gattatagct ttacaaaaga aagcaatgag atagatggct atggtgttga aaatgaagaa
    25321 gtacaaaaag aagggattac ggttgatgag attatagtta tctatacaga tgggagtgag
    25381 gaaaaagtat gacaattact gataagcagt ataaaggaat aagtaataaa gtatattggc
    25441 tagatccaaa acatcctcaa tatagtaaaa gttacaaaga aggcgccatt aaaaactttg
    25501 gagaaacaaa attccaaatc ctaaaaatca aaaactcctt cgacggcatg caggcgatgg
    25561 ctgtagcgcc aatcaagaac ggtaaagtgg atatttctca agtagtgatt gcttttgcag
    25621 gaacgaactt aagcgattgg aaagatttgg aaacggatgc aagaagtgtt atgaatctct
    25681 atgggaatag accaggagca agtttttctg aaaaagcaac aacaaacaag attttatccg
    25741 ttagtggatt gaaaacagcg gggcaaatca attcagcgat tgcatttgct aatgaagtaa
    25801 aaaaggaata tggagaagct gctatttcta ctaccggcca ttcattaggt ggattcttag
    25861 cattatatgt agcagcagag aaacaatgga aaaatgtcgg cttcaacggg ccggacccgt
    25921 atgatctctt atcaccagaa gcaaaaaaat gggtggaaga aaatcccggt atgctcacca
    25981 attaccgaaa tcacgccgat tttatcggta attttggtgg taatggcaca ggtgctgaag
    26041 ttcggatcag tatggaaaca gcatacttga atctgcttgc ttcccacagt ttaacctctt
    26101 ggaagtttga tgaaaatgga aagctgatta ttcctgaaaa tgaaaacaat gttaaagctc
    26161 gaaaacaaca gaaagaaaac caagcgaccc agttgttttc ctatcagttg caagaactga
    26221 acaacatacg taaaaaacta cgattaagtg gcggccggct gacagttaat gaacaaattt
    26281 acttagatga cacacaagct aggttagtgg tggaaaagtt atcggtaaaa atgaaaagcg
    26341 ctatggaaga agttatccgc atcaaccaaa gaggaatttt tgagctagag aaaaaatggc
    26401 aagaaggctt gcggcaagca cgtgttgtcg ccccttcgtt gactgaacag gaaatcatag
    26461 aagcattagc tagtgtgggc gccacaaaag agaacttagt cgattctgcg aaagaagtct
    26521 atctgctacg aatcaataag gcaagacgta tgggggaggc ctttgataca ttggcgaagg
    26581 agatccaaga caaaatcaat gaaatcgtgg cacgagatcg agaattagca cagcaattca
    26641 attaaaggag aagtagatga aactagaaga attggaaaga gattatcgaa gtgaacgaag
    26701 acaattagaa gctcaagaag atgaattgat ccactttaag aatcaaggat tacgattgat
    26761 ggagcaagag gagcagcgac tgcatcatgt attaaagaac ttagctttag ataatgagcc
    26821 tctgtttcaa gcaagacgaa gttattcgaa ttatcaggag cattatcaac aagccctaca
    26881 agtagaacga cgaaaactct atcagcagca agacgaacta gaacgttcct atcgtcaaaa
    26941 aatcagacaa ataactatgg agaaatcatc ttatgaatga atttttaatg caattactaa
    27001 aagaattaga gaaaaatcta catcgatcaa atgtacgttc agttggtatg gggatatctc
    27061 agcaaacttg ggcagaaaaa cgattgacag atcaaaacca ccaaatgact gcagaagaag
    27121 ctactcaaga attttctaac cgacatgcac acttaaaaga atcagtaaaa gggcaatttg
    27181 cagaaaaact aaccgaatta ttccaaagac aagaaaagaa attaaaacag tttcgttagt
    27241 aaagttatat agaaaaattt tgtgaaaatg gtttttcaca tttttacata aatttgtctt
    27301 aatatcgtag atgtaagctt ttgaacacca tttattcgga aaaagggagc tgtgacaaga
    27361 tttttgtcgc agctcccttt aattctgatt aatctattag atcaatcatc tttatcgtct
    27421 gattctttgg tctctggatt cgctctagca taagctgaag cggtgttcca gtaatctgta
    27481 tagttttcat cagtaccgcc aacgccgaat ttaaaggcgc ttttcttcgg accatttttt
    27541 gcccaaagtg attccactgt ttttcctggc gtttggatag attttttatt gacagtaact
    27601 tttccagctg tttgtcctgt gaacccagat acttttttct tcatcacatc ggatgataag
    27661 tcaaatttct catcagtgcc gaaaatctca gggtttgctt ggtaagcttg attgattaga
    27721 ttggcaagat aattagccga acgtattctt gcttggctgt ccattccagt attgtcatcg
    27781 tgtcctgccc aactactgat agtgatagat ggagttgaga cgatcagcca tgaatctcgg
    27841 tattcattgg tagaaccagt ttttccgacc caatctgcat ttcctagatt tccgtttaag
    27901 cttgagatga catctttgaa tggtgtagtg attttttcat tgatcacact acgcatcatg
    27961 tcattcatga tcgatgccgt tgcttcggaa taaatacgga cagaattact ttcatgttta
    28021 taaatcacat tacctgcatt atctgtgatg gaatcaatga tatagccttc ttcgtagact
    28081 ccaccatttg ctaaggcctg gaatccattt gtctgttgaa gcgtcgttac atctactgtc
    28141 cctaaaggag ccgattcaac gccccaatta tcatttgctg ggtagttcat cttcgccaag
    28201 tagtgctcgt aagcgtattg tttcgatcca ccggaattca acacatcttg atataagtga
    28261 taagctggga tattgttgga ccattccagt gactcacgga cggtttggaa ggtgttcgac
    28321 ccttcattgg tcgcattgac gattttctca ccagcatttt ttccttcttg ccaagtcgtt
    28381 gcataatctg atacacggga ttctgatccg acaagtccca tatcgattgc aggaccgtaa
    28441 acaagaaccg gtttgatgga tgaaccagct tgacgttctg tatcaaaggc gtggttattt
    28501 tgattctggc tgtaattccg accacccacg aagccataga tacgtccggt tctgttatcc
    28561 atcaatacat ttccagtctc cacttggcta ccagcaccat catctaacat gtagccataa
    28621 ttttctacgc cggtttgcaa tgcagcatag atatctttat caatcgtcga atggatcgta
    28681 tatcctttgt tttggatcgt ttgctgtgct ttttgataat aagcttcact gaccgatgaa
    28741 tctgacatat cagcattatc tttttctgcc aattgttgtg caataatcct tgttgcttcg
    28801 ttcatcacgg tataatacaa gaattctctg tcagtctgtt ccgcgatttg ttgtgggaga
    28861 aagtcttttg taagatcata ggctttcgct tcttcgtatt gttctttcgt aatttgtcct
    28921 tcacggtaca tactgaataa aacaaaatct tttctggcaa gaccggcaga aagatcttct
    28981 tttagcgcgc ctgtattcgt gtatggacta tacgtgatag ggctttgagg caaaccagcg
    29041 atataagcag cttgcggcag agtcacatcc tttgcatcta caccaaagat tcctctagca
    29101 gcttcctgga ctccagcaat attttgcccc ttattattcc gaccaaatgg tgagacattc
    29161 aaatacgtag taatgatctc atcttttgaa aagtgttttt ctacttgtgc agaaagaacg
    29221 atctcgtttg ctttacgttt gaatgttgtt tcatcggtta ggatctgttg tttgactaat
    29281 tgctgtgtca aagtggatcc tccagacgag ccgatgccag tcgcttctga aactagtgct
    29341 cgtaaaacag ctttagggac ataaccttgg tgtttatcga agtattcatc ttctgtactg
    29401 ataatcgcgg ttttcaataa aggcgaaatc ttatcagatg aaatagtcgt ccgcatcaga
    29461 tctgtctgga tttttgatat ctctgtatta tctgcataaa caatctttga cgtttccgta
    29521 atgtttccta gttctgtctg cagttctttt ttagtcggca attgtgtgtc ttcgacaaga
    29581 taggcaaagt agccagcacc tattccgata cctagagctg cgaacaacaa aagaagaaca
    29641 gtaataaaaa cagtcaatga ttgaaacact ctaaaaataa tcttaggaac aaaccatttt
    29701 tctttttttc ttctattctg tttttttcga gtatttttgc gtggcaaaag ggtcacctca
    29761 actaatggtt ttatttcgct aattatagca aataatagga caaaaaaaca gacaaggctg
    29821 tttttgacag gaatttcttt ttcgaatgag ataaaacgtg aggaaatgat cttttttatg
    29881 aaagagttct taagcttttc tttagggaaa gtttcagtta ctatggagaa cagggcaaag
    29941 aaaaacaaac tattttcttt gtacatcatc ttatcaatat acaggaattg ttcgaaacaa
    30001 ttgcctttca aggaggaatc catccatgtt attaggctca cacgtcagca tgagcggaaa
    30061 gaaaatgctg ttaggcgcgg cagaagaagc agccagttac ggatcttcga cctttatgat
    30121 ctatacaggc gctcctcaaa atactagaag aaaagctatc gaagagatga atattgatgc
    30181 cggaaaagca tttatggcag aaaacggact aacaaatatc gtcgttcatg cgccttatat
    30241 cattaatttg ggaaatacga tcaaaccaga aatgtttcca tttgctgtac aatttctgag
    30301 agaagagatt gtccgtgcgg aagctttagg tgctactcaa atcacccttc acccaggcgc
    30361 tcatgtagga gcaggggcag aagctgggat tgctagaatt attgaggggt tgaatgaagt
    30421 tttgacaaaa gaacagacgg cacagatcgc tttggagacg atggcaggga aaggtacgga
    30481 aatcggtcga acatttgagg aattagcgca aatcatcgat ggagtaaaat tgaatgaaaa
    30541 attatccgtt acttttgata catgtcatac gaatgatgcc ggatataata taaaagaaga
    30601 tttttcaggt gtaatggaag agtttgataa ggtcatcgga ttagatcggc ttaaagtact
    30661 gcatatcaat gattctaaaa atcctcaagg ttctcataaa gatcggcatg ccaatatcgg
    30721 ttttggaaca attggatttg acacattgaa caaaatcgtc catgatccaa tgtttgacaa
    30781 catttcgaaa atattagaaa ccccatatgt cggtaaagat aaaaagaaag cttacgcacc
    30841 atatggaaaa gaaatttcca tgttcaaagc gcaagcattc gatcccgaag tgttccctga
    30901 gttattagca gaagaagtca cactttaagt tttctacctg acagcaacaa aaaaggattg
    30961 ggcagtctgc cttgtccttt tttgtttaag aaacaaagga gtgctttttg cattcgtttt
    31021 cgccatgctc cttttgcccc ctctttttaa attcagacaa acagggtata ctgatagaaa
    31081 agtaaaaaac gaaaagtttc acaaacaagt agcaaaacgt gattgaaagg acgaaaagat
    31141 gctgaacatt ttaaacccct tgatcgacca actaaaaaag aaacatacat actttattct
    31201 tggcatagcc gtattattta caattattcg gttgtattta tatagtaaag caatctatgg
    31261 aacagatatg tcggcgacct atgatgatca gctgctgatt cactatgctc aaaatatttt
    31321 ggatggtaaa tggctagggg agtacacgac tacgacactt tcaaaaggga tttcctacag
    31381 cttgttttta gtacttgcac ataaactgca tctttcttat gcagttttat tcggcatgtt
    31441 cagtattctt gccagtatat tggttgcact agcgtttaaa ccaatcgtga agaacaagta
    31501 tcttctttta agtatctatt tttatttttt gttttctccg attagtttta cacatgaata
    31561 ttccactcga acatatcgta atgctctcgt gatcccgact gttgagatca ttatcgcttg
    31621 tttactagct atttattacc gcaaatataa ttccagcaaa aaattgctgc cgtggttttt
    31681 agggctagct gtttcttttc ctttcttttg gttcattcgc gaagattcca tctggcttct
    31741 tccttttact gtagtcgcgt taggcatcag tttcgttcag atcttgctag gacaagactt
    31801 ttctttcaaa gaagcaaaga agttaaaaga acgaccattt ctcctttcga aacaagaatg
    31861 gctgaaactt tcattgtttt tactccctct agtcggtttc tccatcgtct atcagtggat
    31921 cgaatataaa aacgagacaa catatggtat tgcagccatc aatgaccgtt cgagtggcga
    31981 atttggacgt ttgatgaaac agctgatccg aatagatgac gaaacaaact taaataaaga
    32041 aaacagtgag atatgggtct cgcatgctgc attaaataaa gctttagacg catctcctac
    32101 atttcatact gtttctactc gtattgacga attgtatacg actcatgcgt ggacccaagg
    32161 cggaaaagta aaagaattac caggagatat cattttttgg gcattgcgag atgtggtaag
    32221 cgaatctggt tattaccaaa acaatgcaaa agaaaccaat gatttttgga aaaaagtcaa
    32281 tcaagaatta gaagaagcct atcaaaaggg aactatcaaa aagaaaaaag aattctatct
    32341 gatgggatca ggggatggaa agacgatgaa tgatctgcca atcgtatttg attttctgaa
    32401 atcaggatat gcttatgcat ggcgatacaa agatttccat caagggggga attatagttt
    32461 tgggacgcaa gaacaagtcg aacaggcaca agaattattg aatcttccac taatggataa
    32521 ttggatcgat agcagtgatc cgagaacacc tgatttccag ttgacaaaaa cagcaaaagt
    32581 cgcgaatgtc atgataaaaa tttaccaagt tctggccatt cccgtgctga ttgcttcaat
    32641 agcagggata ttacttatga tactaggaac cgtatttgca aaagctgatc gtgcctatta
    32701 tagttcctct ttgattgtag tcatcggatt aatactgaca caattggttt ttcttttcgg
    32761 tgtctcgtgg ttttgttctt atgcgccaga aaaaaaggat tattttttaa gtgtctacac
    32821 tggcgcaggc gtgccattaa cgcaattcat cgcagtaaca agcatgctag tttttagtaa
    32881 attgaatctg aaaaaagctt tatcttccaa ataaaaaggg aactgtgaca aaagtcttgt
    32941 cgcagttcct ttttccgaat aaacggtg
//
LOCUS       DACKAC010000026        31551 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.026, whole genome shotgun sequence.
ACCESSION   DACKAC010000026 DACKAC010000000
VERSION     DACKAC010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.026"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(862..1203)
                     /locus_tag="I0B46_001276"
     CDS             complement(862..1203)
                     /locus_tag="I0B46_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAR2083588.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(1203..1433)
                     /locus_tag="I0B46_001277"
     CDS             complement(1203..1433)
                     /locus_tag="I0B46_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAR2083589.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            1720..2058
                     /locus_tag="I0B46_001278"
     CDS             1720..2058
                     /locus_tag="I0B46_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083590.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(2263..2502)
                     /locus_tag="I0B46_001279"
     CDS             complement(2263..2502)
                     /locus_tag="I0B46_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HAR2083591.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(2478..2825)
                     /locus_tag="I0B46_001280"
     CDS             complement(2478..2825)
                     /locus_tag="I0B46_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAR2083592.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(3557..4138)
                     /locus_tag="I0B46_001281"
     CDS             complement(3557..4138)
                     /locus_tag="I0B46_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAR2083593.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(4321..4947)
                     /locus_tag="I0B46_001282"
     CDS             complement(4321..4947)
                     /locus_tag="I0B46_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAR2083594.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(4969..6300)
                     /locus_tag="I0B46_001283"
     CDS             complement(4969..6300)
                     /locus_tag="I0B46_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAR2083595.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(6455..6856)
                     /locus_tag="I0B46_001284"
     CDS             complement(6455..6856)
                     /locus_tag="I0B46_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAR2083596.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(6942..7148)
                     /locus_tag="I0B46_001285"
     CDS             complement(6942..7148)
                     /locus_tag="I0B46_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083597.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(7243..8745)
                     /locus_tag="I0B46_001286"
     CDS             complement(7243..8745)
                     /locus_tag="I0B46_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAR2083598.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(8763..9218)
                     /gene="argR"
                     /locus_tag="I0B46_001287"
     CDS             complement(8763..9218)
                     /gene="argR"
                     /locus_tag="I0B46_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAR2083599.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(9316..9933)
                     /locus_tag="I0B46_001288"
     CDS             complement(9316..9933)
                     /locus_tag="I0B46_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083600.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            10154..11527
                     /locus_tag="I0B46_001289"
     CDS             10154..11527
                     /locus_tag="I0B46_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAR2083601.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            11602..12714
                     /locus_tag="I0B46_001290"
     CDS             11602..12714
                     /locus_tag="I0B46_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAR2083602.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(12786..14423)
                     /locus_tag="I0B46_001291"
     CDS             complement(12786..14423)
                     /locus_tag="I0B46_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAR2083603.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAVVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(14591..15199)
                     /locus_tag="I0B46_001292"
     CDS             complement(14591..15199)
                     /locus_tag="I0B46_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAR2083604.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(15583..16734)
                     /gene="queG"
                     /locus_tag="I0B46_001293"
     CDS             complement(15583..16734)
                     /gene="queG"
                     /locus_tag="I0B46_001293"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAR2083605.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(16753..17505)
                     /locus_tag="I0B46_001294"
     CDS             complement(16753..17505)
                     /locus_tag="I0B46_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083606.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(17646..18596)
                     /locus_tag="I0B46_001295"
     CDS             complement(17646..18596)
                     /locus_tag="I0B46_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAR2083607.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(18597..19436)
                     /locus_tag="I0B46_001296"
     CDS             complement(18597..19436)
                     /locus_tag="I0B46_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAR2083608.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(19433..20179)
                     /locus_tag="I0B46_001297"
     CDS             complement(19433..20179)
                     /locus_tag="I0B46_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAR2083609.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            20417..21787
                     /locus_tag="I0B46_001298"
     CDS             20417..21787
                     /locus_tag="I0B46_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAR2083610.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(21842..22738)
                     /locus_tag="I0B46_001299"
     CDS             complement(21842..22738)
                     /locus_tag="I0B46_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAR2083611.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(23019..23696)
                     /locus_tag="I0B46_001300"
     CDS             complement(23019..23696)
                     /locus_tag="I0B46_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAR2083612.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(23787..24692)
                     /locus_tag="I0B46_001301"
     CDS             complement(23787..24692)
                     /locus_tag="I0B46_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAR2083613.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     KNFKNIALDTLKKQLEKQTAVSWTT"
     gene            24867..26321
                     /locus_tag="I0B46_001302"
     CDS             24867..26321
                     /locus_tag="I0B46_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAR2083614.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPSH"
     gene            26337..27368
                     /locus_tag="I0B46_001303"
     CDS             26337..27368
                     /locus_tag="I0B46_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAR2083615.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGHFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            27375..28562
                     /locus_tag="I0B46_001304"
     CDS             27375..28562
                     /locus_tag="I0B46_001304"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAR2083616.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            28718..30100
                     /locus_tag="I0B46_001305"
     CDS             28718..30100
                     /locus_tag="I0B46_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAR2083617.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            30072..30458
                     /locus_tag="I0B46_001306"
     CDS             30072..30458
                     /locus_tag="I0B46_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083618.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(30658..31326)
                     /locus_tag="I0B46_001307"
     CDS             complement(30658..31326)
                     /locus_tag="I0B46_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAR2083619.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
BASE COUNT         8916 a         6392 c         5693 g        10550 t
ORIGIN      
        1 atttttactc attcatagac acttccttaa ataactttta ttatagaagg tattgtaaat
       61 aataatattg acaaaaagtg actcaaaata aaaaaaaact tacattatac tggaatgaac
      121 tgtattgata gtgtacctaa aagctctact gacataagat cgtgtaatcg gtaaagtaat
      181 tttattaatt atacgggatg taatcaatta gagaataaac taatcataaa aggtactttt
      241 acattcataa ttgtcgcttt gagattctac tttggttcac tatctgaccc aattgcacat
      301 tttcgatcat tacaagtatt ggtgtcgtta gaacaactaa ataaaaacta gcggtagatt
      361 tttcagaatc tatgctaatt tctgtatgga tgttccagtg atggcaaatt tctcttttca
      421 ttgtttttct tgtcgcatga tatgcctttt tgtgttattc agttaaacta tagaaattct
      481 ttcctagaca gatcttgatc ccaaatttta cgtttttgag catcgatata gtttaaaatg
      541 acattactca tatctaaaga gtttagattt tttaggacta aaactgtcag ttgttctttt
      601 tcttctttct gttaaagtat tgtcgcgact tttcgtatac gttaagatat gtcgtgaaaa
      661 aacaagtttt gtgacacaaa aaaacgaatg acttgaggaa atcacctcaa tcattcgttt
      721 aaaatgctat atattcttta gtttaagata cggaaattaa tgccatcgac cacactagta
      781 gaatgaacga catttacttg gccttttttt aatttcgtta tgtttagcat ataaaaatat
      841 tatagagttt acttacaatg cttaaaacat atactgtata atctgatcaa ttttagccat
      901 gtcttgcaat ggtaagtttt ctattttttt gagttttcgt tccttaaaat caagagattt
      961 taattgtgta ataattactt gtccttttat atctagctct tcaggtaatg agtaacgtgt
     1021 cggtaaattc tttatagtgg aagtaattgg acatatgacc gaaaacattg ttttacggtt
     1081 aaattcataa cgagagacga ctagtcctgg tcgtcttttt tgaatttctt ttcctacgga
     1141 gggatcaaaa tcaatccata caatatcacc ttttttagga atataaattc tttcttctga
     1201 cactataaaa tttctctccc ttcaggagtt aaatcttccc attcgtcttt ctcataatac
     1261 tcggcctctt ccccaccact aaatggatct tcaatttttg gaactaacgt aattgttcca
     1321 tcatcagaat acacaacgat atattcttgg ttttctgaag gtttctttcc gttatcagaa
     1381 ggtaaagtta ccactacaga acttccttgt aatctagatt ttgtaacaag cattatcatc
     1441 actcccttaa taaaagtata ctatataaca ctaagtaacg caaaattccc ctttgtaaac
     1501 tatctagaaa attgttttgt ttaaagtata tcttattgga tataaataga aataaattgt
     1561 agccagttca ttttttcttt aactaaaaaa ataatccaag aaacattgaa cctatggatc
     1621 caaaagctac ccaaatacta tagctaaccc cccgctacct tcaattttgc agataaaacg
     1681 ctttgggaga aaatttcaaa gacaacaggc catcctatca tgcccgatta taaacaaaaa
     1741 gctggctatt ccttttgctc tgacaaattt caattgggaa agttcacgaa actagcaggt
     1801 gaagaagttt caactgttcg gatcaaagaa tgcccgcttc aaatagaaac attggttcaa
     1861 cgaatttcaa ctagacaaga ctttgctatt gtagaacgtg agatcgtagc tatttttgta
     1921 gaagaaggga ttttacatga tccaactcat atcaatgtgg ataaatggca acctcttatc
     1981 tataaattca gagaatatac aactgccaac cagtcgttaa ctttgaattt tagattccaa
     2041 gaattcaaag agtcataatg ataaacatcg ttaggtataa ataaaaaaga ctcgcagcac
     2101 gagtaaaaaa caaatagaag ctatacatta gaaaggtcga ctgtgaccct atagatgcag
     2161 tatatgcagc ttatgagtat ttcttctgtt ctctttgttt ttgggatctg tgctgaagtc
     2221 tttttcgttt tttgccgctc tttctttgaa aaatcgatta tgttatacct tctccattac
     2281 taattcaggc atctgctgag taatgatttt tcgcgcaaaa gaaatatctg ttacccaagg
     2341 ttcgatttgt tcttcagtca agacaagcgg cattcggtca tggattggtg cgactgtgcg
     2401 gttaggttca gtagtcatta tgattgattc tgccattttt tcatcctgat ggctacgata
     2461 aaaaccgcca atatacacta ttttttcctt atttgaaaac ataaatttgt ctttttcttc
     2521 attccactca ttgggaaaac acaacgattt tgcaaaaatg gttgatgaaa ggtctttttt
     2581 tcttctactg tctctgcacg ggcattaatc atcaattgtc cttttttaaa tccggtaaat
     2641 ccccacttca tgatttccgg agtgaggatg ttttcttcct cttttcttaa cgtcacaaca
     2701 agatttgacg gaaaaacttc tccagctgct aattccgctg cctgcttcgg ttgtttcgtc
     2761 tgggcttctc gataataatt ctgaaggagc tctgactcta gatcaaaaaa ataacgacca
     2821 cacatcctaa tccctcccaa tctttttttc tatcatacac tgcaacaaga agttcgcacc
     2881 aacaaaacgc tttcaatttt tgtagaacta aaaaagttgt gacaagtgac aaactctcca
     2941 gtgaatggta ttctcctgcc aactcctcgg tcacaagtca caatattcaa caaacatgag
     3001 aagctgcttt ttgaaattgt tccttgtgac tcggagtgaa acggcaggct tacgacctct
     3061 gggtaaacgg tgttcaaaag ccagtttctc ggtattaagc caaatttatg caaaaatgta
     3121 gaaagctatt ttcacaaaat ttttcttaat actcgaaact aagcgacttt ttcacaacct
     3181 cttccttaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccaacc aaacgaaaaa
     3241 tatgagcagc tatttttatt tggctgttct tattacttga tgcagggcag ctttttcaca
     3301 acctctccac taaacgatgt tcaatcagct aaccttcggt attagctcaa aaaacggcaa
     3361 aatatgagga actattttgc cgtttgtttc tctaatactc aggtcaaagc gctgatttca
     3421 caacctctaa tacacggtgt tccagaagta gcttcttcga aaaataagcc gaaatttcac
     3481 aaaaatttga gaagcaattt tcgtaaattc cttcttattt cttgaagctg accacttctg
     3541 tcacaacctc taattattaa agaacctttt gcgaagttaa caatctttct ttgatcaatt
     3601 gaaaattctg cctaagtagt gatagatcct gtgtgacttt caacaataag atacttccct
     3661 ggatttcagc aatcaacgct ttggcttgac ggtctgcttc ttcatcggat agtttttctt
     3721 cagaaagtgc caggatattt tgtttcgtat acttgaccca ttgattgact aattctagta
     3781 gcaacagacg gtattcttca tccactgtac tcaactcaat aatcaaatta cctacaggac
     3841 agccatgata agtgatctgt gtctcatgat cagcgaaaat ccagtccaac atttcttcta
     3901 atgcttcttc tggctgtttt ccagcattta agatgtcttc gaacaattct gtctgccata
     3961 cgtccatatg cgcatggatc accgctagac aaacttcttt ttttgaagaa aagtaataat
     4021 aaaattgacc ttttcctgta tcagaagctg ctagaatatc tcggattgac gtttcatgat
     4081 agcctttttt ataaataaga tctttggtcg cttccaagat cttttccttt gttttcaatg
     4141 atattctcct ttttcatttc tcatagtcct atattataca gtaaattcat cattttttct
     4201 tcttaatcgt ttaaaaaaga ctgtgccaaa ttatctgaca cagccctcac tataaaactg
     4261 ttctcatata gctagatgtt cattgattca agaactgcga ggacaaaagc aattcctatc
     4321 ttatgatagt tgctctaata cttcctctgt tgtcatcact tgatctgcaa taaagccaaa
     4381 gttcgtcaac gctgcatcca ttgcttcttg gccaggagca ccaattgcat ctgtcgctac
     4441 cacaacattg aatccactct ctaccaattc acgcatatgg gaatcgctac acaagtttcc
     4501 gttcattcca ccaagaatta ctgtgtcgat tccatttttt ctcaattgga gcgccaaatc
     4561 attgctttct ggtccaaaga ttttatgagg agagctgatg atcgtatttt catccaaata
     4621 aggtttcaat tcttcgataa aatcggctcc tgaaccttct tccactgatt catattgact
     4681 ttttctagca aacatctgct gctcaagcat catatgttct cctgcaccgc caaattgcca
     4741 tttatggtca tgtgggtaaa aataatgagg agaaataaag agtttgtatc ctttttcttt
     4801 tgcttgtttt accatttcaa tcatattttc gatcgtattg tatttttcta agatatcttt
     4861 tgtcaaacca aagccaccgc ctgctggttt caagaactcg acttgcgggt cagtgagaac
     4921 tactgctgta tttttattat tgaacatggt ggattctcca ttcgttattt attttaatac
     4981 atcatttaga gcttcactca ttgtcggatg agaatagatt tgatctctca ataacgtata
     5041 aggaagtttt ccattcatag ctagcgaaat caagttgatg acttcatgag aatcttccgc
     5101 atagatactt gcccctagaa tcaagtcagt ttcagggtcg actaaaactt tgaatacacc
     5161 tttaggatct tctaatactt gtgctttagg gacaccgctt gtcatatact tgaataattt
     5221 gtaattgacg ccttgtgctt tggcttgttt ttctgttaaa cctacattgg ataaagtcgg
     5281 agtgataaat acactgtaag gcacagcttt ccggttattc gttgttcgtt tattctcgcc
     5341 cttcaactga tccataatga ttcgataatc atctagagag atataagtga actgcaatcc
     5401 gcctttgacg tctccaatcg cccaaatgtt gtctgcatct gtacgaaggt attcatccac
     5461 agcgattgct ccgcgatctg ttagtttaac atctgtattt tctaatccaa gtgatgctgt
     5521 acttggtttt cttccagttg cagccaaaac ttttgacgct tggattgtta cttcttggcc
     5581 atcttctgta aaggtgacgg cagctgttga ttcttggtct gctacttgat ctacagatac
     5641 acctagatgg aattcgattc ccgcatttgt catgtcttct aaaatcattt gcgcgatatc
     5701 ttcatcttca cgtggaagaa attcagaatg ggcatcaaga acgacaactt tagatccgta
     5761 ttcattgaac attgatgcaa attccaagcc aatatatcca gccccgatga tcactaattt
     5821 ttccggtaat tgttcctgat ccatcgcttg cgtagaatca atcaagtagc gacttgtatt
     5881 caatcctttg attggtaaaa tgattggttc agaaccagta ttgatgaaaa tccgttcgcc
     5941 ttcaacaatc actttttcac cattattcgt cacttcgatt tgatggttag aaagaaaacg
     6001 tgctgtaccg tccatcactg tcccagttgc ctcatcgctg atcatatgat aatttttggc
     6061 gcgaagcatg cccgtcaatt tttctttccg agcaacagcc tctgtaaagt cgattcctct
     6121 ttctccattg aaaatcaatg atttagaagg aatacagccg atgttgatac atgttccgcc
     6181 atacatacgt gttgactttt caacgactaa aaccgattca ccttttgaag ctaacgcctt
     6241 cgctaaagtc ttaccgccct ttccaaaacc gatcacaata ttttgatatt tttccatcat
     6301 ttttccctcg cttctttttt acctaccgat tggtacaaaa atatgataac cgataattgt
     6361 ctattctcca aatcatctgc tttatttgtc aaaaaaaacc agacaaaaag cggtcctttc
     6421 acgtttttag tctggttcga tttcctttat tcttctaaac aatcgttact tctacatcga
     6481 tatttcctct agttgctttg gagtaagggc agaattcatg cgcatcttct gcaatttttt
     6541 gggcttcttc ttcgctgatg ccatcgatat gcacttctaa tactacaccg atctgatagc
     6601 cgccatcatc taaattataa agagagacac gtgcttttac tgtggactct gctgtcattc
     6661 ctgcttcttt gatcatatgt tgtaccgccc cattgaaaca agaagcataa cctgctgcaa
     6721 ataattgttc tggatttgtt ccttctgcat ggattcctgg aggagtgatt ttcatatgca
     6781 tcgagccatt tggtgcctcg acttctccat tgcgcccgcc atggttgatc attgctgttt
     6841 cgtatgcttt tttcattgta ctgctccttt cgattgacca tttgctactt ggctataatt
     6901 atagtgtatg acaagtaagg cgctttctac aaaaattctg cttattcgaa taccagaatg
     6961 ccgtcttcta ctatttttcc aaaaaacaaa aaaatactac tcgtgaacaa cagcgtcata
     7021 tttgttccaa aataaatagc aatcttttct acataatctt ttatatgatt gattttaaga
     7081 tacatatata ccagaaagtt gaccccataa accaatactg ttaaaaaaat aaccatattc
     7141 gttaccattt tttcctcctt ttgctggtct ccctaccagc actcgattta accataccac
     7201 tttttttgcc gtccaagcaa ctagcctttc aaaaagactg gtttattctg ctgttaatga
     7261 ttcaagatca gaaaaatgtc cgaataattt tgctaatttt actaacgcca acttgttctt
     7321 tccgaattca tcattttcta tctgtaatac tagtaacggc tcatgtaagc ttaaacggat
     7381 caaaaaccag ccgtttccgt atggctcttc tagatcatat cgaatacctt cttcattttc
     7441 cgggtcgaag ttcatccctt tttcttcttc caacagtcgc ttcatttctt ctaatatctt
     7501 gtttccttgt tcgcgtggtt catcgctgaa caattttaat cggatttctt gtacttctac
     7561 gggctgtttc aactctttga tttgttcatc taatgtgata ccttttcttt ttaattctgg
     7621 caaaagcatc aggatctttg cgatgacata tgcaccatca tccagaaaat agttttcctt
     7681 gaatgcagcg tgtccgcttg tttcgatggc tagctgtgta tctttcccaa gacggttaag
     7741 ttctaatgct ttattgatta catttcgata gccggaaata taacgaactt ggatgcctcc
     7801 tttttcttca atgaatcttt gtagatgaga agaagtaggg gaattcgtca cgattcttgt
     7861 tcctggatgc tcttttaata cgattgtact taatactgcg atcaaattat ttcgattgat
     7921 cactgttcca gaagcactga caactgcaga acgatccaca tctgtatcga aaatgatccc
     7981 aagatctgct tcatttgcta aaacagcctg cttgatactt tccatcgctt ctttattatc
     8041 aggatttggg atatgattag gaaatcggcc atctggttcc aaaaattggg aacctgttga
     8101 atccgcacct aagctattca atactttttc agcaaagaat ccaccagcac catttccggc
     8161 atcaacaatg attttccaac catttagtgg tttgctttct agtgttccaa tccccttttt
     8221 aatttttgtg actaaatctt gcgcataagg tgtcaaaagg tctgcttttt ctacgcttcc
     8281 agtttgttcg ctttttattt tttgctgctg tgataggata aaagcaatat cttctttttc
     8341 tgctcctcct tctgaggtaa agaattttat tccattataa taatagggaa gatggctagc
     8401 agtcagcata accgcagcat cgcacgagaa ctgttcaaat tgagtggaca taaacatagc
     8461 aggcgtagta gctaattcga aatcgatgac atgtatccct tgttctttca gactttggat
     8521 caacgcctct ttcagtgctg gtccgcttat cctactgtca tgaccaatcc caatcgtcca
     8581 ttttttattt ggatcggttg atttcttctt tttctttatc cagtttgcta gtccccagcc
     8641 gatctttttt accatgtctg tcgtcaagtt tacttcatat ccttcaactg gtaatgcgat
     8701 tcctcgaata tccgatccat tttgcagcgc tttcatatct ttcatataaa agtcctcctc
     8761 atttattccc tcattttttc cagcttttct gcaaatcgct gtgcttcttc ttctgagcgg
     8821 caaataataa tgaatgtatc tacaccggcc atcgttccga caacttcagg gtaagccact
     8881 tcatctagcc aattcgttac cacatcggca ccatttcgtt cagtcaatac gataaccatg
     8941 aactgaacag actggatacg caaaacctcg cgtctaattg ctgtacgcaa acgttcttca
     9001 tttaaagaaa cagatggctg gttaaataat gtatagcgta ctattttgtt ttcattttga
     9061 ctttttacta acttcatttc tcgaatgtct cgagaaatcg ttgcttgagt cacatcggcg
     9121 cctgcttcat gcagccgcgc aagcagctct tcctgtgtcc caataggttg ttctgtaatg
     9181 atctggcgga tcagtgcttg tcgctgtttc tttttcatat tagacacctc tcaatgttga
     9241 ttatagcaaa agataagtaa tatttcttca tattttggta ttttttactt ttattttttg
     9301 ttttcattca ggttgctaac ctttttctat tttaacacgc aaaatcgtct tcattttttc
     9361 aggtgctgat tttcttggat cgctgatgta aatctctttg tgttctttag aagctctttc
     9421 atagccttct tttgcgatga atgcttctaa tttctcaaac gttgctggtt cttcggcata
     9481 gggcccgacg tgtaagattt gggcacaaag accttcttcc atctttttga atactacttg
     9541 ttctttcaag tcttccggaa gtttgctaga acgctgaatg gcttcttgtg ctatcgcttc
     9601 tgtaacaaag tcaggctgtc ggatcatcaa ttgataagaa aaatgttctt ttttcatcac
     9661 tggttcatgc aaatactttt cttgcagtcc ccattgtcct tccagtggat agacagtata
     9721 catctgataa tcaggaatca gccaattttt tttaggcgac atgcgtattg catagctgac
     9781 tgcatataga caggctactc ttcgtttaaa gtcttcctga ttcgggtcgc cgatccctct
     9841 gatgctaaag aatgattttt taggaacctc tagtatttgc gggctttttt tcatactata
     9901 gctcttgtct ttacgccatt caattttttc catttttctt cctccgcgaa attccattta
     9961 gttatttatt atcatacctc tttttgagtg acttggctaa tcgtgttgac actatttttc
    10021 agtttgtgct agaataagtg aagtaaataa atcgtacaga taacacgttt cggcgtgtcg
    10081 agagagaggc ggtttctatt tttataatag aaatcactct ctttttttac gaaatttttt
    10141 agaggtgatg taaatgacaa aacaaaaaca gctgctttta gtacctgcag taattgcaac
    10201 agggatgatg tcttttgcgg gcgtattgat cgagacggcc atgaatgtaa ctttcccaac
    10261 tcttatcaga cagtttgatt taacgactgc acaagtccaa tgggtaacaa cgatttatct
    10321 tttgatgatt tcgatcattg ttccgctgtc tacttatttg aatcgcaatt ttagtctcaa
    10381 aaaactcttt ttagcttcaa atctgctttt cttggcaggt ataatgatcg atttcttctc
    10441 tcctaccttc atggtcctat tgtttggtcg attgctccaa ggtgcagcga caggtatcgc
    10501 tcttccgttg atgttccata ttatcttgac atttgccccc ctcaatcgac gaggcgcgat
    10561 gatgggtgtc ggtactttga caacggccat tgctccagca attggcccca cttatggagg
    10621 cattatgact tcacatttta catggaatca tatcttcctg tttcttgtgc ccgttttgat
    10681 tctttctttg attatcggct ggtttgctat tcccgagatg gcagtacaaa aaagtggtta
    10741 tttggattgg ataagcgttt taggaatttg tcttttgttt agtggaagtc taacattttt
    10801 gagtacgttg ggaacagttt ccagctggat tgctttaatc atcggcggta ttggctggtt
    10861 ccttttttac tatcgaagta aaaaagtgga gcatccactg atccgactgt ctattctttc
    10921 aaataaaaca ttccgcttgt ttttgattgg cttcttagtt tatcagtttt tattacttgg
    10981 tgtatctttt gtcttgccga atttccttca gatcgttcaa ggaagtagtc cttttgtcgc
    11041 tggattagcg atgcttcccg gcgcagcggt tggtgctttg ctttctcctt tttccgggaa
    11101 aatgctggat caatatggtc cgaagaagcc aatttttgct gggctgattc tttctttgat
    11161 tggttggcta gcattattcc tactcatagg acacgcttca gtgggtgtat tgattgcttg
    11221 tcatgtgttt tatatgatcg gtatcggatt atcttacagc aatatgatga ccaccggaat
    11281 gaatgcactg aatgaagaac tacaaggaga cgggaacgca atgttcaata ccttgcagca
    11341 gttttctggc gctgtggcta cttcattagt agcggtcatt atcaattacg tccagcatca
    11401 tacttcccat aactatgaag tgtctactac attaggatca aaagcagcat taggcgtctt
    11461 gttgctgttg cttctcgtaa gctttgctcg attcgcctat tatctgttct ttgctaaaaa
    11521 agcgtagatt ccatatacgg caggtgttgc atctgccgtt tttttgtgtg aaaatggttg
    11581 tttgtaaaag gaggcttttt tatggaaaaa attgccatta tcggtggcgg gatcattggt
    11641 atgaccctag ccagttactt agatacatca aaattcaatg ttgctttatt tgatgaaggg
    11701 acaggacagg caactaaagc tagcgctggg attatctccc cttggttgtc gaaacgcaga
    11761 aacaaacaat ggtatcgtct ggctaaagac ggcgctgctt ttttccctaa acttataaaa
    11821 gatttccaac ttgatcaaac gatttacgca caatcaggga cacttttgct tcgacctgaa
    11881 aaagatttag ctgaattggc tgttcttgcc gagaaacgaa aagaagaagc gccagaaatt
    11941 ggaaacatcg aatggctttc acctgaacag acatctcaag cacttccgtt attgaaacca
    12001 gcagtttcac tgcgtatcag tggtggtggt cgtctggatg gaaaagcata tttagaacat
    12061 cttggtcagc gtttagcaca aaaacaaatg gagcagatcc aggcaaaagt cactttgcat
    12121 aagaagagcg attcttggct catcaaggga ccgtttggag aatacacagc tgatcgtgta
    12181 attttgacac ctggcccggc acttaagcct ttattacaag aattaggcta tgctgcagat
    12241 atacgtccgc aaaaagggca gctcttagca tttcagacat cttataagat gagtcaagac
    12301 tggcctgttg ctatgcttga cggagaagct gatttcattc ctttcatgga tggaaagatc
    12361 cttttaggtg ctacccatga aaacgatcaa ggctgggatt tggctcctac tagcgaagct
    12421 tataagggat tgactaagag tacagaacag ttcttgactg atccagatca gataaaaaac
    12481 tggccatacg agttccgtgt aggtacccgt gcttatacgt cagatttttc cccatttttc
    12541 ggcgtactgc cagatgatcc aagcttactg gctgctagcg gtctaggctc ttctggcttg
    12601 accacaggtc cttatatcgg ctatctatta gccaacttct taaatactgg tcattggcct
    12661 acggaagatt atcaaaagga catctccacc tatattacga aaaatccatc atagaaaaac
    12721 agctttttca agtaaggatc ccgagcgata ttgcgcatgg attacttctt gaaaaagctg
    12781 ttattttagt tcaatttctg aatcaaattc tccaaacttt cttttgcttc atctggcaag
    12841 agatcttttc ctatctgagc ttcaatgaat cgtaaacgaa tatcaagtcg ttgtttcacc
    12901 agatttttat aggacgtatc ctcaaaagaa acaggaaaat ctggtatagg taaatagtgg
    12961 atgtcttcaa atggaccaaa cgacttgaat tctgccgttt gttgcacgat tctttcaacg
    13021 attgacaatg tcgtttcagg tttgattttt ttatcatcga aatatccagt attcaggaca
    13081 tagcaggctg tttgctgttt caaaaacaac tctcgaaaag cttgatagtc ttccctaaga
    13141 ggatagacac ggaaaggatt agcaaatggt tcaatgacta gctcatctag ctgtcttttt
    13201 gtagtgatat tttccgcgtc tgatcgtttt gttgctaatg ttgcaccaaa agacgctgct
    13261 aaaactgggt cattgatctt gataattggc ggaagcgaat catctttcat gatccaaaaa
    13321 acagcatcga tcttttcgct aagatgatcg actcggtcgg gtgtcgcata acgtgatttg
    13381 actgtccgcc catttccgtt tcgtatatct cctgttacta ggactttttc tcctttactt
    13441 gtacatgtca cgcccacatt ctgacaagta aggaaagaag aaatggccgg atcatcagac
    13501 ggatagtctt gtgttttatc aaagtaagaa ggttccaagg cagtagcaaa accatttttt
    13561 cgagaaataa cgaaagcatc gtcatgcaag atcgaaacag agtgaccttc cgtatgtttt
    13621 gctaaggtaa tcgttgattt tccagaaccg gacaacccaa atacagccat cgtataggtc
    13681 tggtcattac tagcatattg tttcatccca ccgtggcagg caataaagcc attgcgattt
    13741 cccaatgccc aagcaagcgt caaagtcgct ttttttaatt ctccaaagta acgtaatcct
    13801 agcacaaccg ctgtattatg gagagcatca aacaaaatga gtccatttgg aaatgtttca
    13861 tgtgtccatt cgggatcaca aaagatataa agatctccct catcaaagga aacagacttt
    13921 tgataagtct ctaagatctt cgaattcgct ggctgaaaat tcagcaaata agaatacaaa
    13981 ttgttctcaa atccttctgg aatcagcaaa tgactttgca cagaaaattc ttcagatagg
    14041 ccaacgatta cttttgtatg gtaaaactgg cgcttgcgac tttgatacac agcttctctt
    14101 aaaattttct cgtatttgct ttgttccttg tctaagtcct caaagatccg ccgagcactt
    14161 gctgttcttc ccacaatgct tccatcattg aatactagtg tcctaacatc agctggtaaa
    14221 tctagttctt cagcatgtga gattgttaaa tctgtgatga tcgttcctgg actccctttt
    14281 gctagttgat aagctgtctt cctatcaaaa acttcatgaa cattattacc gtaaaatgtc
    14341 gtttctacaa tcgatcgaat actggataac agtggatttc cacttgtgat ttcgtttaat
    14401 tcgagactct cttttgtact catggctcct cctcattccc taaacaaaaa aacgttttct
    14461 ttattataca ttattcgagt ttttatttgt actgctgcgc tgtgggaagc aaacgagtta
    14521 tttttcacac aaaaaaaccg agaacttgaa taaacaagtt tctcggtttc atcattttcc
    14581 ttttatcggc ttagtaccag ccgtttgcta accagaatgt tttagcagca tcccatgaac
    14641 cataacgaga tgttacatat tgttctgcta cacgttcttg attggcagct gaatagtcgc
    14701 cgtttaagta tgaagcatct aattgataac ggccgatgta acgtccgtta gtagctgtgt
    14761 atgaaccgcc tgattctttt tgtgcgatcc attcttttgc agagcttgaa gttggcgctg
    14821 ttgcagcagc tggggcagca ggagctactt caactgtttc aacaggtgca acgtatgtat
    14881 gttcttgcgt tgctgtttca gttgtagatg ctgtattttc tgttgttttg ttttctgttg
    14941 ttgctgaaga atcatttgaa gttgggattg ttaattgttc accaacataa atgcggttga
    15001 tgtttgcgat cgaatttttt tcagcaattg catcgatcat tgagttgtct cctgcaaatt
    15061 tttgtgagat cttagataat gaatcacctg attttactgt ataaacttca tcagcgtgag
    15121 catttgtagt tcctacaaac attgcagtag caccagcagc aaaagttgta cctaaaacaa
    15181 ttttcttaaa tgaattcata ttaaactcct ttttttctcc gttttttatt ctttcgacgc
    15241 tattaacttt atcatttaga aatatttcat aggtaaaaga aacatgacat ttaatgacaa
    15301 aataacgaat tatttaatat ttgtaacatt taaacaaata tttttttgat acaaaaccat
    15361 aaaagccttg ttgtataagc aaagtaatcg tttttctatg tttttttatt tttgaatgaa
    15421 aaacgcagat tctgtaacat tcactgcaat aatccataaa aaaaagcaaa aaaatttttg
    15481 atttttatca aattctctta aaaaaatgta aaaaatacaa aaaaaccagt aattgttaca
    15541 acaattactg gacaaaaact ttgaataatg ttaactattt ttttatttta actttctccg
    15601 caataatttc tgggcatgtt ccaattcgtt tataacgact tcttctgttt ctttagctaa
    15661 tatttcatct aatacatcaa gccatttttc cggttctttt gttcctagac gtccaatagc
    15721 ccaagctgct gttcctctga tgacagggcg gacatctgtt aaacattctg aaatctcagg
    15781 caatgccgtt cgatcaccta gattagctaa agcaatcaat gcgtttctct gcaaaggttt
    15841 cttcccacgc catgaaccag ctaaatgccc aaattgctgt ttgaagtctt tattagacat
    15901 cgaaagcatt ggctttaatt ttggatatat ttcgtctact tttggttcca tctcttcatg
    15961 aaaatgaaaa tcttttcctt gattgtaagg gcagactaac tggcagatat cacatccata
    16021 aatgacattt cgcatttttt tacgatattc ttccggcatc atcccttttg tctgagtttg
    16081 ataagacaaa catcgctggg cattcattct tccgtcacca agcaatgctt ttgtcggaca
    16141 agcagtcacg cagcgcatac agtcaccgca accaaattct cctggtttat caggctcaaa
    16201 tacaatatta gtaacgatct caccaagata gacaaatgaa ccgaactctt cagtgatcaa
    16261 caatccgttt cgtccaataa atcctaaacc agctcgctgt gctacagcga catcgatcag
    16321 ttctccagta tctacttgcg gagctagacg ccattcctct tctttttgcc atttttctgc
    16381 tcttgtttgg atgaattcga tcaatcggtt caaacggtct ctgagtatat catggtaatc
    16441 gatcccccaa gaagcacgag caaactgccc gcgtttttca tccctaggca cttcttcatg
    16501 gatcctagta ggatacgcaa gtgcaatgga aatgatcgat ttcggctgat caaatgtcat
    16561 ttctggataa agacgctcat cgatatttgg atgttcaaag ccagaagtat ggccagctgc
    16621 tttttgttct tccaacgatt cttttaatga atcaaaaggt tccgccgtgg tgaacccgat
    16681 tttgtcaatt cctaatcgtt tgctttcttg tatgatttct tcttttaatg tcatggtctt
    16741 cctcgcttgc tgttaacggc tgtataaccc ataatatagt ttttcttttt tcagctggac
    16801 aaactggatc tgctgatcgg caatcttctt ttcttccatc catttttttc ttgcagcaaa
    16861 tacttcccaa taatttgttg aaattaattt tcttttgtta tctaatgcat taaaaaaatt
    16921 caatgaaact ggttttgtac ttaacagttc atggagcctt acctcttccc atggcgaaaa
    16981 ctcagacaga tcgcgggaat acaatacttc ttcatagaga tttttttgct gtgtgtacgt
    17041 gaatgtttgc gggattttcc caggcctagc aatttctact agacgattat aaaacatatt
    17101 cattaatttg ctgttggcaa taaattgact tttataatag gccgactgga tctttttgat
    17161 tactccttct gctttgatga ctgaaaataa tgcaggccat gctttgacgg ttatttcgtc
    17221 gttttgttta actaatacgc caggtgtgaa gccgactgga gaaaggaaca caaaataacc
    17281 gataccggta ttttcatcta aaggctgaaa ttcatcaaac aaccatttgc ctatgtacag
    17341 ctcttcatct ggttctgttg tctcattttg atttgctatg aataatttag ctaattctgc
    17401 ttcaagaaat tgaaggtctt gatccttgtc ggtattttcc tgctggctga tcaatcgaaa
    17461 gacttcttct ttaatcgtat atttatcttt acgtgtaaaa ttcatagcca caccttcctt
    17521 ccttactcta actataacgg aatttcttca aaaaaggtat ctgtacgacg aaaaacagca
    17581 taattttttt tcatatctct tataaaaaat gcaaggtctg ctcctcaagc tagaccttgc
    17641 acttttcagc tttcttttgt ttgtgacaaa ccttcatgga tcgtttcaat attccatttc
    17701 atcatctgat agtaagaatc tccttgttct ccttcttttg ctatggaatc agtgaacagt
    17761 ttatcataga ttttcaaacc cgtttctttt gctacccgtt ccatgcttct tgagtctaca
    17821 ctggtttcca cgaataaaac cggtacttct tttgctctta tctgatcaat gatcgctttc
    17881 atttgatcag gcgtaccttg actttctgta ttgatctccc atatataagc tgctggcaaa
    17941 tcatatgctc gtgaaaaata cttgaatgcc ccttcacttg ttactaataa ttttttgttc
    18001 tcttcgatag aagcaaaact ttccttcgct tgtgtatcta attcttttag tttttccaca
    18061 tatgcttctg catttttttt atagagtgtc gcattttctg catctttttc agagaatata
    18121 cgtgcgattt cctccacata ttttattccg ttagataggt ctagccaagc atggggatct
    18181 gcttttgtat gttcttcacc gctagttaaa taaagaggtt ctacattttt gctaactgca
    18241 aaataatctt ttccttcttc ttttttagcc gtttccatca agttatcgaa ccagctgtta
    18301 cctgtttcaa gattcaaacc gttgtataaa ataacatctg catcgcttgc ctttttgatg
    18361 tcttctggta gtacttcata ttcatgcgga tctgttccga ctggtacgat actgtggata
    18421 tctatttgat ctgttccgac ctcttttgcc atgtccgcca agatcgaatt ggtagccacg
    18481 actgacaact tttctttact tccttcatcc gcttggtcag aagcctgctt tccgcaaccg
    18541 gctaatacca agaaactaac tgccaagatt aaaaagagtg atttttttgt tttcatctat
    18601 ctgcttcctt tctgaaataa aattcctttt gttggagaaa ataaaaaagc aaggataaaa
    18661 aatgcggtac atgctaacac gatcgtagct cctgatggcc aattgtaact ataactgaaa
    18721 aacacaccga caactgcact tagcataccg attcctgcag ctgtaatgat cattttcagt
    18781 aaatggttcg ttagcaagta agccgtggcg gctggtgtga tcaacatggc aatcaccaag
    18841 attgttccta ctgtctgtaa actgactaca gccactaatg tcaaaaagaa catcaaaaga
    18901 taatggatca gcgatgtgtt cagcccataa gcttttgcca ttgtcggatc gaatgatgtg
    18961 atcttcaatt ctttataaaa gaaaaagaca aatatcaaga cgattccact aacgagtgct
    19021 gtcagcaaca aatcgctttc cctaacagct aagacattcc caaataaaat atgatacaaa
    19081 tctgtcgcac tctgggcata tgaaatcaag ataatcccta atgcaaaaaa ggagctaaag
    19141 acaattccaa tcgctgtatc atttttcaat cggctccgct gagtcacgaa accaatcact
    19201 gctgctgtta gcatcccaaa agcagtggct ccaaagatgt aactaaaacc aaacatataa
    19261 gaaatggcta cacctggaag aactgcgtga gagatagcgt ctcccatcaa agacatccct
    19321 cgtaaaataa taaaagaacc gatgactcca gaagcaagtc ctacaagcat ggaagtgacc
    19381 aaagcgtatt gcaggaattg ataattcatt aatccatcaa taaaggattg gatcattttt
    19441 tctctcctcc ttttactaaa tgtctgatga tctcaccata ggccaattga aggttcttcg
    19501 atgtaaagac ttcttgtact ggaccggaag cgatcagctg tttatttaaa agaataacct
    19561 catcgaaata ttcttctact ttatgaagat catgatggac gatcaaaatc gtttttcctg
    19621 aattcttcaa ttcctgcaag atgtcaaaga tctttcgttc acttaacgca tcaatcccta
    19681 cgaatggttc atctaaaaag atccattctg ctccttgggc tagagctctt gcaataaaaa
    19741 ctctctgaag ctgtccaccc gatagttcgc tgatctgtct tttggcatat tcttccatcc
    19801 ctactttttt caaagcttgt cttgcacgtt ctttttcttt tttcccaggt ctttgtccaa
    19861 ttcgtaaaga tggatatgtt cctaaaagta caacgcctaa tacatcaatt ggaaaggaaa
    19921 gatccaattc acttcgctgt tccacgtagg ctattttttt acggactgag tttaccgact
    19981 gtccagaaaa ggtgacttta ccagtagtag ctggtatcaa ctctagcaaa gatttcataa
    20041 atgttgattt tcctgcaccg tttggtccga taataccggt aattttttgt ggatttatcg
    20101 tcaagttgat agttttcaaa acagttttcc cttgataagc ggctgaaagg tcttgaatta
    20161 caatggccgt acgttccaaa tcttacactc ctttttaggt ttacctaact ttttatttaa
    20221 atatagttta actaaaatgg aaagcatttt caagtagaat gatgaaaaag tttttttcga
    20281 attttcctcg cattttatga aaattttctg tagaatcaca tcaagagttc aataaggcta
    20341 acctaataat gaaaaacatg tgttgaaacg tttgattatt ttttcttgtt gactcaacaa
    20401 cgaaaggagt ttttgtatgg aagaagaaaa gttaactcga agtcttagtg cccgtcatat
    20461 ccaaatgatc gcactaggtg gaacgatcgg tgtcggtctg tttatgggtg cctcctcaac
    20521 catacgttgg acagggccct cagtgatggg agcctatgcg attgcaggat tctttttgta
    20581 tttgattatg cgtgctttag gcgaaatgct ttacgttgat ccttcgactg gttcttttgc
    20641 aaattacgcg agcgagtaca tccatcctgt ggctggctac ttgaccgctt ggagtaatat
    20701 ttttcaatat attgtcgtag gaataagtga agtgattgct gtaggatctt atatgaatta
    20761 ttggtggcct gatttgccgg caattattcc cggaatcatt gtcgtcttgt ttcttatgct
    20821 ggcaaatctt atttctgtca aggcgtttgg cgaattagaa ttttggtttt cgatcatcaa
    20881 agtcataacg atcattttga tgatcattgc aggtcttggg gtaatcttat ttggatttgg
    20941 taatcatggt caggcagtag ggatttcaaa tctttggaaa aatggtggat tcttcactgg
    21001 aggcgtcaaa ggatttttct ttgcattatc gatcgttgtt gcttcctatc aaggaatcga
    21061 actgatcgga atgactgctg gtgaagcaga gaatcctaaa aagacgatca ttgaagctgt
    21121 ccagtccact atcggtcgaa tcttgatttt ttatattggt gctattttta tcattgtgag
    21181 tatctatccg tggaatcaat tgagtgagat tggttcacct tttgtacaaa ctttttctaa
    21241 agtcggcatt acatttgctg caggactgat taattttgtc gtgattacag cggcattatc
    21301 aggttgcaat tcagggattt tcagcgcaag tcgtatggtc tatacacttg ccataaatgg
    21361 aaagatgtct aaaaaatttc tgaaactatc tcgtcacggt gcgccttttt atcctgtcgt
    21421 agcaatctca ggtggaatct taattggtct gatcttaaat tatcttctac caatgttcat
    21481 caaccagtca gaaaatattt tcgttttcgt atacagctca agtattttgc ctggtatggt
    21541 tccttgggta gtgattttga tcagcgagat tcgcttcaaa aagaagcacg cagataaaat
    21601 gcatgatcat ccttttaaaa tgccactatc tccttatacg aattatgtga cacttgcttt
    21661 tttagctatc gtattgattt tcatgtttat caatcctgaa acgagaatct cattgattat
    21721 tggactcgtt ttcttgcttt acatgacgat ccattacttt gtacgcgaaa aaagaaccgg
    21781 aaaataaaaa aagaacgaag cttcacctta gtggaagctt cgttcttttt tattgctttt
    21841 atcaattcaa ttttagcgtc tcaccgggag tcaggaaata attctccatg ctcatcccat
    21901 tcagcgacaa gatcgtttct actggtacgc cagttcgttc ggctatttga tttggacctt
    21961 ctccttccaa aactacggtt gtcgcagcct gtgaagtcgc gctgttggta ttagtttcag
    22021 aggaatctgt tgtgtcttgt gttgttcgag cttgcatttc agaagcttct accgactctg
    22081 ctgtctcaga atcagaagat gttgtgtctt gtgtgcttgt tgactctgct tgaagagagg
    22141 cgctagtctg caaataaata tcggaaatct tagcgacatg attcgtataa ttagccgata
    22201 attgattgat caaagtatct aagtctacac cgtctttatt ttctgctgcc tgatacgctg
    22261 cacgtaattt tctgatccct tcgtcggaga ttgcttgaag ctcacgtgct ttttggttag
    22321 caactgctga taaaccagct attccctctt gatttccctg aatttctgtc tggtattctt
    22381 caatcaatgc aggcgtcttt tcttcgatct ttttcgaata atcatttacg attgcttgaa
    22441 gttgctctgg caaaccattt gctgtttttt cttcagaagt ttgtgtctct tttgttttag
    22501 ttgatgactc acttgtttgt ttcgtctctg ttttctttgt cgttgtttcc gttatggtcg
    22561 tacttttcgc ttctgacgaa ttttttccat gaaaatatag acccgcaact cctcctgcta
    22621 tgatcaaaaa cacggcaact gctgccccaa tcatgatcat ccgcttacga gctgcctgtt
    22681 tttgctgtct tcttcgggtc atccgaacat tttctcccca ctcattttca tcatacattt
    22741 ttttcaccac caatttttat gtataatagc gttatcaaac gcttcatatt cttattgtag
    22801 caaataattg tcgctgtgca catttttatc tttgttattt ataagaaaat ttcaccagtt
    22861 gagatgattc caagattgtc cgtcgatttc tccttgcagc tgcattgttc gttcgtctat
    22921 ctcagccatc agtttcttta tttcatataa gagcgcacgg tcttgatatt ctgatttttc
    22981 gcttaattca tctaattgtt ggatcagcca atcatttgtc ataatgaagt tcctttcatt
    23041 tcttgcagtg taaaggtcaa gtcttcgatc tgctcttttg cctcagtcag tgcttctacg
    23101 atatatgctt gtttttcaga ttcttcttcc tgcattttct gattttcctg tactctttga
    23161 ataaaccagt caatccgctg agtcagcgat ggtttgcctt ccaatgagag atactgctga
    23221 taagcaatta gcttctccca acgtttttcc tgggataaat acataaattg tgctaaaacg
    23281 aatggcggaa gataggtcat atggaattta tttgcgattc gctgatacgg cctcaaaaat
    23341 tcggaaatcg tatattcttc ttttcctcca gcttggtatt ctttttccgc cacacctgtt
    23401 gtcaatacga ttcctagttc tttccctttt aatacatgtg ctgctcgttc caagacatta
    23461 tcttgccaaa tttttaaatg agcaggagaa ctataccagt aaaacggaaa ctggaaaatg
    23521 atccgatcat gctgggccaa gagctgtagt tcagtctcta tatctatttt tccgtctgga
    23581 taacttgatt ccaaatgatg ccaagtagct ggtaaaccag cagaagcttc tttgaaaaat
    23641 tgctgattta aagattcttc gatcgttgga tggctaatga tgataagtgt acgcatccct
    23701 tttcctcctt tttagtcttc gctttacagt gtagcataac cgccacaagt ttcttttgcc
    23761 gatgcttgtc attttaacca gactaatcag gttgtccaag aaactgcggt ttgtttttcc
    23821 aactgttttt tcaaggtatc caaagcaata ttcttaaaat ttttgttttt catacgttta
    23881 cgatagttcg tagccgtttc tccggtactc tttttgaaca ttcggctaaa tacgactaga
    23941 ctattgaacc ctacttgata ggcaatactt tccacggatt catccgtcag agaaagaaga
    24001 aacgcccctc gattgatccg taccccgatc aaatattcct tgatactggt acccatatga
    24061 tttttgaata ctcgcgacaa atggctccga ttgaccgcta aatgctgggc gatttcctca
    24121 acagacaatt tttcagcata atgtgcctcg atgtatttga ctgtttcaac agctaaagag
    24181 gaatgaacct tttgtacacc accgctgacc tttccgccat cttttaaaag gactgataaa
    24241 aaacggtagg ttaatgctgt taactgtaat tcattttcca aggtttcttc cgtatctttc
    24301 atgcattcta aaataatgtc gatatatttt tgtatgtcag aagagaccat cgtgtattgg
    24361 tcttctctga aaagcgactg ccgaatgatt tcttctgcag cactcccacc aaatgaaagc
    24421 cagcaataca tccacggatc ttctccatct gataagtaaa acgtcgagtc tcctggacga
    24481 atcaagaaca gatcaccttt tttcaattgg aattgttgat ttttcacatg ataagatcct
    24541 ttgccttcca tcacgacatg caggatgtaa tcattgcgaa tagcaggacc aaaagaatga
    24601 aacggcatcg ttttagagaa accacaaaaa tcaaaataca attctttttc tacttgttga
    24661 tgttcctttt ttaaaaaaat cgattgctcc acgtctatcc ctccttactc ctttagtata
    24721 atgcaacaaa aagttaaata aaagtcacag aaatatatta aaaaggaagc gatttcttgt
    24781 taagtttaag tcattaaaag atctttgtta ttcgttgtaa ccggtttact tagtattata
    24841 tattgaacag gagaggaata cagattatga aaagcaaaat gatccaacgt gtgtcctacg
    24901 ctttaggcgc ttttggacac gacacattct atgttacatt aagcacttat tttatggtat
    24961 tcgtgacaag ccagatgttc gcaggaacag ataaggcaac gaatgcaaaa atgattgcta
    25021 cagttacaag cttagttgta ggtatccgct tagttgaaat tatttttgat cctttgattg
    25081 gtggcatcat cgataatact cgtacaaaat acggtaaatt caaaccatgg ttagttatcg
    25141 gcggcgttgt tagttccgta ctcttggtag caatctttac caatttcttt ggcttagcta
    25201 ctcataatac gactttattt atcatattat ttgcaatcgt tttcatctta ttggatagtt
    25261 tttattcatt taaggatatc gctttttggt caatgattcc agctttgagt acggaaacaa
    25321 aggaacgtga aaaatttgct acgtttgctc gtttcggctc ttctctaggt gcaaatggta
    25381 ctactctagt cgttgtccct atcgttactt tctttaccta tcttgttact cataaacata
    25441 ttgaaggcgc ttcaggctgg ttctggttcg gtgtgattgc tgctgttatt tcaggcggta
    25501 ctgcgattat cactgccttg ggcacaaaag aagtcgatac tgcgatccgt cagcaaaatg
    25561 aaaaagctgg gatcaaagat gtgttcaaag cagttgctca aaatgatcag ttgatgtggc
    25621 ttgcccttgc ttatatcgct tatgcgattg ccaatgcagc aacaactgcc gtattattct
    25681 acttcttcaa atatgtactt ggccaaccaa ctgaattttg gatcgttggt ctagttgctg
    25741 ccttacttgg tttagtagct gttccactat tcccaatgct tacacgcgtc atctctcgtc
    25801 gctacgttta ccttggaggg atcgcaatga tgatttcttc ttactttttc tttacgatcg
    25861 caggttcaag tttgccaatc gttatcatcg ggctagtatt gttctacttc cctcaacaat
    25921 tgattttctt atccgctttg atgacgatca cagactctgt tgaatatggt caatggaaaa
    25981 atggtatccg taacgaagct gttactctat caattcgccc tttactagat aaaatcgctg
    26041 gtgctttgtc taacgggatc gtagggtttg tcgctatcgc ttctgggatg actggtaatg
    26101 ctactgctgc tgatatcact gctcatggcg taacgacttt caaagcttat gcattctatg
    26161 ctcctgctgc tttgatgatt ttctccgcta ttatttttgc ttggaagatt acattgacag
    26221 agaaaaaaca tgcacaaatc gttgagaaat tggaaaacaa attggctcca gctgatacaa
    26281 tggaagatga acttcacgat gcagtagttc cttcccatta agaaaggatc ttgaaaatga
    26341 agattacaga aaaagatttt ggccaaggtt atcacttgat aaccttggag aacaaaaaca
    26401 agctagcact ttctatctct gatttaggtg ctagaatcgt cagtttaaaa tcaaatgatc
    26461 gagaattagt tttgggattt gacaccgctg aagaatatat cgaaaaagat ccgtatatcg
    26521 gtgcttctat cggtcgcacc gcagggagaa tcgaaaatgg ccacttttct ctcaatggaa
    26581 aaacttacca attagccaca gatccaaaaa ctggacataa tctgcatggt ggtgctcctg
    26641 gatttgaatt aaaaaaatgg tcttatgtca ttttgaatgg cgaaaatgaa gcttctgtca
    26701 tttttactac tacaagtccc gacggagaac atggttttcc aggtacaatg gatgtggaaa
    26761 tccgctatac tttgacaaaa gataatatct ggcgagtgac ctctcgcggc accagtgacc
    26821 aagatacttt attcaatccg acaaatcatg tttacttcaa cttgacggga gatgcttctc
    26881 agtcaatcga tcaacatgag ctttggctca atagcgaagc ttatgctcct ttgcgaacag
    26941 atagtattcc tataggggta aaagaaaatg ctgcaggaag tgctttcgat tttcaaatac
    27001 ctaaaaaatt agcgtccgtt tttgctagtg atttggatca aaaaaatctt gttgacggaa
    27061 tcgatcatcc atttttctta aaagaaaccg gcttgggtaa agaagccgct cgcttgacta
    27121 gccccgataa aagaatccaa gtagatattg ctacagatgc ttctagtgtg gttattttca
    27181 ctgcaaattt cggcacagaa acaccagaaa tgcgcaaccg aaaactggca catcacggtg
    27241 gcatcacatt tgagactcaa acagctccag gtgcagagcg cttctcagca tttggttcga
    27301 ttcatttaaa agccggatca gtatttgaaa cagtgacaga attcaaaatc aaaacaagaa
    27361 aggaataact catcatggaa atcaaaagaa gcttaaacac aaaatttgcc gaaacattcg
    27421 gacctaaaaa aactacacaa tacttctcac caggacgcat caatttgatt ggtgaacaca
    27481 cagattacaa tggaggccat gtcttccccg cttccatcac ttatggaacg caaggtgtag
    27541 cagcacctcg agacgataat aaaatattag tttattccac aaactttgac gatatcggtg
    27601 tcattagttt tactttagat gagctagaat atgataaaaa agcaaactgg gcaaattacg
    27661 taaaaggaat ggtccttaaa ttaaaagaag ctggacactc aatcgatcat gggtttgagt
    27721 tattagtgga aggaacaatc cctaatggtg caggactttc cagtagcgct tcattagaat
    27781 tattagtagg tgttgtatta gaagatctgt ttgatttaaa aacagatcgc ttggaactag
    27841 tccaaacagg taaaaaagta gaaaatgaat tcatcggtgt caattcaggt atcatggatc
    27901 agtttgccat cggcttcggt gagctagatc atgcaatctt attagataca aatacgctga
    27961 aatacgaaat ggttccagtg aaactagatg gttatgcaat cgtcatcatg aatacgaata
    28021 agcggcggga attggcagat tcaaaataca atgaacgccg tagcgaatgc gaagaagcct
    28081 tggcacgttt gcaaaaaact ttgcctatct cttctttagg agacttagat gaagaagaat
    28141 ttgaatcaac tattgaccaa atcggcgatg atacattgat acgcagagct cgccatgccg
    28201 tttctgaaaa tcagcggact ttgaaggcaa aagctgaatt agaagctggg aacttagaag
    28261 catttggtca actgttaaat gattctcacc actctcttcg ctatgattat gaagtaactg
    28321 ggatcgaatt agatacacta gtagatgctg ctcaaaaaca agaaggtgtc ttaggtgcac
    28381 gaatgacagg agctggattt ggtggctgtg cgattgctct tgtcaaagaa gaaaatatac
    28441 ctgccttcaa aaacaatgtc tatgatgaat acatgaaagt tgtcggctat gctccggaat
    28501 tttatgttgc acacattgga aatgggacaa caaagatcga tccagaagct gttcatgttt
    28561 gataaaaaaa tcaacgatct cagctaaaaa caaatagata aaaataacct ctcagcaatt
    28621 tccaaatgtg agccttttgc tcgtatttga aaattgctgt tttttattga cacggaaaga
    28681 gaacgtgtgt tctaattgtg ttgaagaggt gattgttatg tgggcgaatc aaaatccggt
    28741 atttgtttat gaaaaagaac cttcaagaga tattttatgt attgactgca aatcttttta
    28801 cgcctccgta gagtgcgttg aaaaggatct tgatcccctt acaactaaat tagtagtcat
    28861 gtcctatccg tccgattcga cagaaacacg gggtagcgga ttaatcttgg cttccagtcc
    28921 tgcagcaaaa aaagcttacg gcatcaccaa tatcagccgt gccagagatc ttcccttccc
    28981 ctatccaagt gatttatata ttgttgcccc aaggatggcc tattatatgg aaaagaacag
    29041 agaaatcaat tctatctata aaaaatatgc agacgaagcg aaccatcatg tctattccgt
    29101 cgatgaaagc ttccttgatg taacaaatag catggcactc tttggtaaaa caaatgcgta
    29161 cgacatggct aaactgatcc ataaagatgt gtttgagcag accggaatct acacgactat
    29221 aggaattggc gataacccgc tacttgctaa actagcctta gacaatggct caaaagacgg
    29281 aaagcaaatg tttgctgaat ggcgttatga agatgtacca gaaaagttat ggaaaatctc
    29341 agatatcact gacttttggg gaatcggcaa acgaacagca aaacgtctga aacaaatggg
    29401 tatacattct gtttatgatt tagctcacgc ggattattat cacttgaaaa gcaagctagg
    29461 ggtcatcggt actcagcttt atgcccatgc ttggggcatc gatcgaagtt ttttgggaca
    29521 agaatataca ccaaaatcaa aaagtatcgg aaatagtcaa atcctagata gagattatac
    29581 aagacgagaa gaaatcgaga tcgttatcaa agaaatggcc gaccaagtcg gcactcgctt
    29641 gagacgatct gcagcaaaaa cacaagtagt cagcttatgg gtaggatact ctttaggcta
    29701 tcttgatcca aatggcaaga ccgggtttca ccagcaaatg aaaatcgaag cgacaaatgc
    29761 tagcagagat ctagcagatg ctttattgac cattttccac cagcattacc actaccagga
    29821 tattcggcgt gtaggcgtga actgttccga cctcgtatac gcgacaggtt tacagcttaa
    29881 cctttttgaa gatcctgaaa agcagataaa tgaagcgaaa gttgattttg ttgtcgatac
    29941 gattagaaaa aaatacggct ttcaatcaat catccatgcg catagtctct tgcaaggagg
    30001 acgcgccatc gcacgtagtt ctctcgttgg cgggcatgca ggtggtatga gtggattaga
    30061 aggtggaaaa gatgagacgg acagcaaaaa aattcattga ctataacgag tatcgagatc
    30121 ggccatttgg attaaaatgg gggactgctt ttgcgatgga agaactagtc aacggtatca
    30181 aaaaaaatga agaagaagca cgcaaacagc ctcttcctaa acaacaaatg acattgcaag
    30241 aaatcgatag tatactgaca gaagcctttt tatctcgtaa agcagtaatc attcagctca
    30301 atttaaaaga tgaatttgga agactcttgg atgaggtcga agggcacttc attggtgaag
    30361 cctatgaaga ttactttatg attgaccatc agccgatatt gtgggaagac gtacggtata
    30421 ttgagttaaa ggaaacaaaa aaatggtttg tgacatgaga ggttgtgaca gaagtgatca
    30481 acttcaaaaa ataagaaagg atttttcgaa aattgcttct taaatttttg tggaattttg
    30541 gcttattttt cgaagaagca acttctggaa caacgtatat tagatatcag aggttgtgaa
    30601 aaagtcgctt agttttgagt aacaagccaa ggcgctgatt tcacaacctc tgtctatcta
    30661 tccatctatt cggtaagatt catgcatgac gtcttcttta tttagtatgt agagcccttt
    30721 ttcttctgac cattcaacat aaatcagctg ttttacatct gcatagcctt ctttcttcaa
    30781 gttctccata agccatgctt cagtcaattg attggactga agcactttat gctgaatttg
    30841 cccttcatct acaagcagga tcgataaagc tttgtcttca tctgattttt tcaatacact
    30901 catttgaccg gcattctcta ggataacatg tttggcattt tcaagaaaaa agcattcttg
    30961 ctgtcttaag attgctcgca actgctccat ctcgatatgg ttatttgtca actctgtcat
    31021 gttgatgacg ccgtctttga ttagaacaga tggctcgcct ttgacccaac gattgacctt
    31081 ttcatttttt tggacgatac gttcgatacc ataaatcatt actgcccaaa gcgcaatggc
    31141 aaataggaca tgccctacat ggacattgtc atcataggtt gattcttcta agatcccacc
    31201 taaaacaagc gtatagacta aatcaaacgg tgtgatttct gacatggatt tcttcccaag
    31261 caaacgtacc actaataata aaccgatcaa cccgaacacc agtttcattg taattgaaaa
    31321 aatcattttt tctcccccta actatctgtt aattaaatta tatatgcttt ctcacgaaca
    31381 cgcatggaat atacaaggaa gaaatgtatc cttttactaa aaaaaggact gtgacactgt
    31441 agtgaccccc aaaagttaga cttttttgga gtggagaaat ccactccttt tttttatgct
    31501 ctaaaatgat atttttacac aaccgttacg gttgttttac gaaattggac g
//
LOCUS       DACKAC010000027        31117 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.027, whole genome shotgun sequence.
ACCESSION   DACKAC010000027 DACKAC010000000
VERSION     DACKAC010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31117
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.027"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(334..696)
                     /locus_tag="I0B46_001308"
     CDS             complement(334..696)
                     /locus_tag="I0B46_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083620.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(770..1150)
                     /locus_tag="I0B46_001309"
     CDS             complement(770..1150)
                     /locus_tag="I0B46_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAR2083621.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1232..2407
                     /locus_tag="I0B46_001310"
     CDS             1232..2407
                     /locus_tag="I0B46_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAR2083622.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2484..3068)
                     /locus_tag="I0B46_001311"
     CDS             complement(2484..3068)
                     /locus_tag="I0B46_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAR2083623.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3269..4267
                     /locus_tag="I0B46_001312"
     CDS             3269..4267
                     /locus_tag="I0B46_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAR2083624.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4379..4888
                     /locus_tag="I0B46_001313"
     CDS             4379..4888
                     /locus_tag="I0B46_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAR2083625.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4918..5577)
                     /locus_tag="I0B46_001314"
     CDS             complement(4918..5577)
                     /locus_tag="I0B46_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAR2083626.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5579..6343)
                     /locus_tag="I0B46_001315"
     CDS             complement(5579..6343)
                     /locus_tag="I0B46_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAR2083627.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6351..7028)
                     /locus_tag="I0B46_001316"
     CDS             complement(6351..7028)
                     /locus_tag="I0B46_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAR2083628.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7065..8450)
                     /locus_tag="I0B46_001317"
     CDS             complement(7065..8450)
                     /locus_tag="I0B46_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAR2083629.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8660..8744)
                     /gene="ffs"
                     /locus_tag="I0B46_001318"
     ncRNA           complement(8660..8744)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="I0B46_001318"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8767..9282)
                     /gene="tadA"
                     /locus_tag="I0B46_001319"
     CDS             complement(8767..9282)
                     /gene="tadA"
                     /locus_tag="I0B46_001319"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAR2083630.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9389..10246
                     /locus_tag="I0B46_001320"
     CDS             9389..10246
                     /locus_tag="I0B46_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAR2083631.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10327..10662)
                     /locus_tag="I0B46_001321"
     CDS             complement(10327..10662)
                     /locus_tag="I0B46_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083632.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10729..12099)
                     /locus_tag="I0B46_001322"
     CDS             complement(10729..12099)
                     /locus_tag="I0B46_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAR2083633.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12177..12713)
                     /locus_tag="I0B46_001323"
     CDS             complement(12177..12713)
                     /locus_tag="I0B46_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAR2083634.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            13022..14503
                     /locus_tag="I0B46_001324"
     CDS             13022..14503
                     /locus_tag="I0B46_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAR2083635.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            complement(14606..15313)
                     /locus_tag="I0B46_001325"
     CDS             complement(14606..15313)
                     /locus_tag="I0B46_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAR2083636.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(15392..15730)
                     /locus_tag="I0B46_001326"
     CDS             complement(15392..15730)
                     /locus_tag="I0B46_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAR2083637.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(15841..16803)
                     /locus_tag="I0B46_001327"
     CDS             complement(15841..16803)
                     /locus_tag="I0B46_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAR2083638.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(16818..18149)
                     /locus_tag="I0B46_001328"
     CDS             complement(16818..18149)
                     /locus_tag="I0B46_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAR2083639.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(18157..18837)
                     /locus_tag="I0B46_001329"
     CDS             complement(18157..18837)
                     /locus_tag="I0B46_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAR2083640.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(19056..19535)
                     /locus_tag="I0B46_001330"
     CDS             complement(19056..19535)
                     /locus_tag="I0B46_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAR2083641.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            complement(19662..21341)
                     /locus_tag="I0B46_001331"
     CDS             complement(19662..21341)
                     /locus_tag="I0B46_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAR2083642.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            21889..23391
                     /locus_tag="I0B46_001332"
     CDS             21889..23391
                     /locus_tag="I0B46_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAR2083643.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            23404..24066
                     /locus_tag="I0B46_001333"
     CDS             23404..24066
                     /locus_tag="I0B46_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAR2083644.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(24167..24370)
                     /locus_tag="I0B46_001334"
     CDS             complement(24167..24370)
                     /locus_tag="I0B46_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAR2083645.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(24481..25665)
                     /gene="iadA"
                     /locus_tag="I0B46_001335"
     CDS             complement(24481..25665)
                     /gene="iadA"
                     /locus_tag="I0B46_001335"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAR2083646.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            complement(25822..27561)
                     /gene="yfcC"
                     /locus_tag="I0B46_001336"
     CDS             complement(25822..27561)
                     /gene="yfcC"
                     /locus_tag="I0B46_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAR2083647.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            complement(28334..28948)
                     /locus_tag="I0B46_001337"
     CDS             complement(28334..28948)
                     /locus_tag="I0B46_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAR2083648.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(29554..29931)
                     /locus_tag="I0B46_001338"
     CDS             complement(29554..29931)
                     /locus_tag="I0B46_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083649.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(30231..31112)
                     /gene="galU"
                     /locus_tag="I0B46_001339"
     CDS             complement(30231..31112)
                     /gene="galU"
                     /locus_tag="I0B46_001339"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAR2083650.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT         9285 a         6465 c         5191 g        10176 t
ORIGIN      
        1 aacgactcag ttatcttatc atgtcgttag taacatgtca ataactattt tttaaatttt
       61 ttagaaatgt gatataacat ccacttcaga atcaacgctg ttaaatttat catgtatttc
      121 ttccttggtc aactataaat taagcattac tttgtgaaat gatttttcga tcaattatta
      181 ttattcgtta tacaaccgtt ttcaacatat tttgaaaata aaatgcacta aaaaactatc
      241 tttagcagaa catttcattg tattttttgg accaaacaat aaaaaaagaa gtttgaaaaa
      301 aattcaaact tctttgttcg cttaatctgt attttagaag aaaggcactc cctctacttc
      361 gtaagttcca agaacagagc gcatttgacc tggttcttcc caaatatatc cttcgcctaa
      421 ttcttctctt tgattctttt gttcagtttc aaagacaaac aacggaacat tttcctcttt
      481 aatatgtgta tttgtcaatt ccatcaaact aatcttgcta acatcgatat gaactatttc
      541 ttttaagaaa ttaagtatat tcgctaaacc agtctttcct tcagaaagtt ccgctaacgc
      601 aaattccaac ttgtcatcaa ttgaatggac caaaaatttt ttagtaccat cctccaaatg
      661 caacataatt gtaccagcaa ctcttttgct tgccaaaatt tccacctcga tcaacttgtt
      721 ctatggctta cataaaaatt ctagcacatt ttcagaaaaa atacactttt tatttcttat
      781 tttctttccg ttctagttca gataatgctt cttctatcca aataggtaac tgctccgcaa
      841 tagtggtaaa tccgatctta cgatttttat tcgtgaaata cctttttctg cgatatggaa
      901 cagctggatt tgttttttct aacgttctta aagataaact gatttttttg gaatattcat
      961 caatgtcgat gatctgaact gttactggct gtccgacttt taacaatgaa tggatatttt
     1021 tcgtataacc ggattgtact tctgaaacat gaataagtcc ttgcgtctcg ttatcaagag
     1081 aaacgaatgc gccataaggc tgtatccctg tcacttttcc ttcaattatt tgtccaattt
     1141 tataagtcat ctacttccct ctcttcgcat ttatcattat actgtaaata gctcaaaaaa
     1201 agaagcgacg aaaatgactg gaggtcttaa gatgatcgaa tttgataaac agatttcaag
     1261 aaaagataca gaaagtgtaa aatgggacgc aattgctgag acatatcaaa tggatgactt
     1321 acttcctttg tgggtagcag atatggactt cttgtctccc ggtggggtag ctaaagcgtt
     1381 tagtgaatac atcaaacatg gaatctttgg ttatgccact ctgtctgata aactatatca
     1441 ggctatcatt cattgggaac gccaacacca tgctgtggag ttgacgaaag aaaatatcgt
     1501 ttttacaagt ggtgtgttaa ccagtttagc tgcagcagta caaactttta caaaaccagg
     1561 cgattctatt ttgattcatg acccagttta tcctccattt tcctctatta tagaaacaaa
     1621 tcaacgtcac atcgttcgaa gctctttgct agaaaaaaat aatcatttca tcatggactt
     1681 gtctgacatg gagaaaaaaa tcgaagaaaa caatgtaaaa gcaatgattt tatgcaatcc
     1741 tcacaatcct ggagggcgtg tttggagcaa agaagaattg aaacagttaa gcgaattgtg
     1801 tttaaaacat caagtgattc tgtttagtga cgagatccat caagatttaa ctttattcga
     1861 tcatacattt acttccatgc taacgattga tcctaattta gatgatctac tagttgcttt
     1921 tacttccgca accaagactt tcaatctagc tgcaatcaaa aattcaatgg tatttatcaa
     1981 aaatccagag atgaaagctg attttgaaaa acatttagtg atgaaccaac agcacgaaat
     2041 caacactttt ggactgattg gaacccaagc ggcttatgag acgggtggag aatggttaag
     2101 ccaactgatt ccttatctag aaaaaaatgt tcaaacagtt aatcagtttt ttgaacagta
     2161 tcttccgaaa gtgagaataa tgaaccctga aggaacttac ttaatgtggt tagattttac
     2221 cgcctatatt tcagacgacc aagaattgga gaacactctt gttcggaaag gaaaagtcgt
     2281 attaaatcct ggcatcacgt ttggtccttc cggtcatggg catatgcgct tgaatattgc
     2341 ttgtccagaa gaaactttac tggaaggact taatcgaatc aagtatgctt tagagacaat
     2401 cgattaaatc atttattcaa aataaaagga cgagacctta ctaggtttcg tcctttttca
     2461 taattactcc gtaccatacc aaactattct gaaatttcaa ttgtttcaat cactacatca
     2521 tgtacaggtc ggtcttgtgg accacgttgt acatttgcga tttcatctac tgtgtccatc
     2581 ccttctagta catgtccaaa gactgtatgt cggaaatcta gccaaggagt tccgccacct
     2641 ttatacgctt caatgatttc tgatgggaat ccagcacctt ctagttgtcc aagcatatta
     2701 gctggcacat ttgtgttatt tacaataaag aattggcttc cgtttgtgtt tggacctgaa
     2761 tttgccatag ataaagctcc gcgaaggttg aacagttcac gactgaattc atcttcaaaa
     2821 ctttcgccat aaatactttc gccacccatt cctgttccag ttggatcgcc gccttggatc
     2881 atgaaatcag gaataacgcg gtgaaaaatc acgccatcat aataaccttt tttggaaagt
     2941 tcaatgaaat tttgaaccgt cttcggtgct agttcaggga ataattgaac agtaatgtca
     3001 ccacggtttg ttttgataac agcttttggt cctttttcac tttttaaatc tagttgtggg
     3061 aatgccatat tgtttcctcc taatatttgc atacttcata ataataataa cataatacct
     3121 aacctctcgt aaaaatacta gatttgtttt cttttatttt tcatcactag atacattcta
     3181 taacttaccg tttcgatttc gaattcaaac atttttgaga agcattctca attaaattct
     3241 gctatagtgt gaaaagggag gaatttttat ggaaatctat gacattacaa tcatcggtgc
     3301 aggtcctgta gggatgtttg ccgcttttta cgcaggaatg cgccaagcta aaacaaaaat
     3361 cattgacagt ctacctcaac ttggtggtca attagcaact ttgtatcccg aaaaatatat
     3421 ttacgatatt cctgggtatc cagcaataaa agcaagtgaa ttaatcgatc aattagaaaa
     3481 acaattaact acttttaacc atacattcca tttaaaagaa gaagttctct cgcttactcg
     3541 tgaagacgaa gttatcgaga ttactacaaa taaaggcatc cattattcca aagcagtcat
     3601 tttagcttta ggaaatggtt cattccagcc aagaaaattg aatttagata atgctgaatc
     3661 atttgaaaac cacggattgg attatttcgt gaatgatttg atgaagtatg ctggaaaaaa
     3721 agtcgctatt gctggaggtg gtgattctgc tatcgattgg acgttgatgt tagagcctat
     3781 tgcaagcgaa gtttacttga ttcatcgtcg cccggaattt cgtgcgcacg aacatagtgt
     3841 atcacgcttg aaatcttcat ctgtcaactt gcttactccc tatctcatcg atggattatc
     3901 aggcaataat ggggaactga cagatatccg gttaaaaaaa gtgaaaagtg acgagacgat
     3961 tgatttgatg attgattctc tgatcgtcaa ttatggattc tcttctaatt tagaacatct
     4021 ttcttcttgg ggacttgata gtaccagaaa tgccattact gtaaaatcag atatgtctac
     4081 ttctattcca ggcgtttatg cagctggtga catctgtacc tatgaaggaa aagtgaaact
     4141 gattgctact gggctcggtg aagcacctac tgcagtgaac aacgctttac actatattaa
     4201 tccaaaggaa cgaactcagc ctggtcacag cacaagctta tacgataaaa atcttcgtcc
     4261 tagttgattt tttatatctc tttgcatttc aagttaaacc cgaaagcagt ttcttgcatt
     4321 tttttcatgt tccgttaaca tgaaatagag aaagaaaaaa taaaggggga gcaataatat
     4381 ggtcgtcaaa actgagactt tagaagaaaa ggcaagagaa ctattgaagg aacgtggcgt
     4441 aactgtagaa gatatcgcac aacttgtcat gtttttacaa aaagactata tttctgattt
     4501 gactttggcg gattgtactg aaagtgtcaa cagcgtgcta acaaagcgtg aagtacataa
     4561 cgccattatc actggaatcc aattagatat attagcagaa aagaaaaaat tgatgagtcc
     4621 gttgcaacat attatcgaag atgacgaagg cctttacggt atcgatgaga tcctagcctt
     4681 gtcaatcgtg aatgtttacg gtacgatcgg ctttacgaat tatggttata tcgacaaggt
     4741 gaaacctggt attttaaaag aactaaacag ccacgatggc gttcatgtcc ataccttctt
     4801 agatgatatc gtcggagcga tcgccgctgc tgctgccagt cgattagcac acaaagaacc
     4861 agaaaaaaga agtcgcctta ctcaataatt caacaaaaaa agaaagaaaa cgtctattta
     4921 gacgagttct ttcttttttg tttgtttcaa gctatttttt cctaagaaaa gaaaaacagc
     4981 aaaaaataat tctaataata caaaaaataa gataaagcta tgcatgaaaa attcttctgg
     5041 aagtacattg atgattggat cagtaacggg atcgaataac cagtcatcgt tgttgaaaaa
     5101 cgtttcatga aaaaggataa aaaagcgatc aaatcctatc atcatgaaaa aaccgaaaac
     5161 aactggcagt aacatgccga tttgaaacgg acgaatcaac cgccaaagcc gatcattctt
     5221 ttgcaaatat ttgataaaca aaatactcgg gatcaaagtg atgaaaaaca cagcataatc
     5281 cactaaaaac aaaattttca cttcataaaa gtgatgggct ccgctagcag aaactgggaa
     5341 atctggcaag ctgaggatgg ttttgaaggg attattgaga taattcatta ggtgatcaaa
     5401 atttttcagt aacgtctctt gatctaaaga agtataatcc aaaatttgca aatgtccgat
     5461 atcaaatacg tatagtggtc gaaagttgat cgtcacacca actgccagac tgatcagtgt
     5521 cagaaatagg ctgaacaatc cagcatattc catccactgc catctcgaat ttttcatatt
     5581 agaagctcca ttcatccagt gattgtttca cataggtagc tgggataggc aacccaggta
     5641 catcttcttc ttttgtaaaa cctgataaaa cgagcaaagt atctatatta ttgcgtatcc
     5701 cagcttggat atctgtttca tagttatctc caaccatgat tacttcttct ttttgcagcc
     5761 ctaatacttc aacggcttta tccataatga tggcttctgg ttttccgatg tagatcggcg
     5821 gcgtttgggt tgctgtctct acaaaagaga tgactgaccc tgctccagga agaaggccgc
     5881 gttcagtagg tatattctta tctgggttcg ttccaatgaa agtagcacct ttttgaatag
     5941 caagtgttgc tttaactact ttttcatagg tcagataatt atctaagcct accactacat
     6001 agtcaggagc ctcttcttcc catgtaaatc cggcagctaa aatcaagtca atcaatcctg
     6061 cttctccgat aacatagact tttttccctt ttccatctgc tttcataaaa tcgattgttg
     6121 ccaaagtagc tgtgtagact gtctctggag ctacatgaat gtcaaactca tctgctagac
     6181 gattagctac tgtttctggt gacttagtcg tgttattggt aacaaacaag aaaggtaatt
     6241 ttcttttttg caattcttcc acaaatcgtt taccggccgg tatgggttgt gtcccacgat
     6301 aaatcgttcc gtctaaatca attaaatatc ctttatagtc cattcatcca ctactcttcc
     6361 ctttttctga tcgtaaatgc tgtttttgtt ttttctactt gtttaggttg ttcttgtttt
     6421 tttacttttt cagaggtttt tgctgactct gtttttcgat ttttaataac cggttttttg
     6481 cgattagatg cgactggtcc ttttttctca tcgatatacg cacgattatg gttttttttc
     6541 ttttttcggc gctgagttgt cttttctttt ttaccgccga ttctctcgat gacaaaatac
     6601 gcacaaccaa aattgcaata ttcgtaaaga tagtcttcca aggaatcgat tcgctgatct
     6661 ggatgcgccc gtcgattatc cgcttggaaa aatcctttta ggcgtagttg ttcataaccc
     6721 caatctccta cgatataatc atatcgggct agtacatcac tgtaacgttc tccaagcttt
     6781 tctgcattaa atccttcacg ataatcggac acaagccgat aatgtctttc acctatcaaa
     6841 aaattactgt catctaacag tgtcactagc tcgcctttga ctttttctgc tttaggctcc
     6901 tcaacgattt cttccaaagc agctgtcagt tcttcggtga ctgttgtttc ctgttcagct
     6961 gatgattttt tcttagcaat ttctgtttta tgctcaacgt tctgctgttc attttgtttt
     7021 gcagacatgc gttcaccacc tatctttttt attatacctt gtttttattt gattgggtag
     7081 tgagctttca agtaatctcc taccacactt cggataaatt cagggaacaa aaattcattt
     7141 tttccagcaa tctcaatcgt tggaaaaaat ggaacaaaca tcaaatgatc tacggtagta
     7201 aaagtataat ttttattcag atcgattggc ttgttcttcc aaaagacttg atggttaata
     7261 gaatcatatg tgattccatt atagaccatc tgcccaaaaa tcttaccgcg aaatcccatt
     7321 cccttcatag gatagttacg taaaaagtta cgattttttt ctatttctaa aactaagcgg
     7381 atcagatcat ttccacccaa tgtgacattt aacaagtgca tcggatgcgg caacgcagtg
     7441 tgaagctgat cctgattgac ccgccctttt ggaatttcag ttaaaaacag accactgttt
     7501 aatattgctg cttccgtctt tcctcgttct ttcaccgcct caaaggctac ttggataaac
     7561 gaatgctcat tgaagatatc tagactcaga gaaaatggca aatctgctac ttctttttct
     7621 tttaataatt catgcccacg ttctagataa ctatttattt cttgttcatc ttctgtaaaa
     7681 gctgtcattg tttcggtagg gatggctttg gctgaatgct taaggatttt tttctcttca
     7741 tcgatggtta aatggacttc accgacatac tgcccgtact ttccagcggc tgctatttgg
     7801 acatcattga ttacgtgacc ttctctgaat aaatgatggg tgtgcgatcc taaaataaca
     7861 tcaattgctg gtatttcttt tgcaatttgg aaatcatcct gaatcccgag atgactcatc
     7921 aaaactaaaa catccacttg tgaacgtaaa tcttcgacta attctggcaa aatatcataa
     7981 ggattccgta tgtcccagcc attcggatta taagtaagcg gaaatggagc agtaaatgca
     8041 attagtccga ttttcgtctg gtgtttcgtt gtaatgattt tatatggttt cgtccatgcc
     8101 ggtggctgga gcgtattttt atcgaataag ttatccagga tgatatcaaa ctcagcatga
     8161 tcgtataaat gattcaactc ttctttggaa tttcctacgc cttcattgtt tcctatcgtc
     8221 gctgcatcat aaccaacttc attcatcagc aagatatttt cctgtccatt cgttgcttct
     8281 gacaatggat gccaacgatc cacaaaatca cctagatcta ctgcaatcgt tgtcgttttt
     8341 tctttctttt cgttttctcg tttacgttga tccaaaaacc ttctgatcct tggccaattt
     8401 tctaaatgag aatgcagatc attcgtatgt aaaatcacta ttttttccat gtaccctctc
     8461 gcctctcttt atctttatca taccataggc agctttttct tccattgaaa caagctctat
     8521 ttcagagttt ttcctgcctt gaagcaaatt gtataagtac aatattcgat aaatcagtct
     8581 ggctaaaaaa tcaggaacat tcatttttac agtactataa aaataaaaaa aacagacccg
     8641 aaagtctgtt gtcgtttcat tataatcaga cacatggcgg aacctgctta gtgctgcttc
     8701 cttccagacc tgacacgctt cacaagccca ccattgtcct agcctttcgg caaagaatag
     8761 tataccttat ttttcattcg ttgaccagtc tttttttaat ttcttttttc gctggcggag
     8821 ctttcggaaa aagtcagtca aaatcatccc acattcgtct tctaaaatgc caccttctac
     8881 atatgcttgg tggttaaacc gctcgtcctc caaaagattc atcaaggtac cggctgttcc
     8941 gccttttgga tcatacgcgc cgaaataaac ttctgctaca cgagacaaaa tcatcgcgcc
     9001 gctgcacata ggacaaggct ctaaggtaac aaaaagtgca gcatcttcca agcgccagct
     9061 tcccactttt tcacaggctt ccttgattgc aagcatttct gcatgtgtag tagcatcttg
     9121 cgtggtttct ctaaggttat atcctcttcc aatcactttt ccatccaata caaccactgc
     9181 tccaatcgga acttcatgaa gcatttctgc tttctttgct tctgccaatg caaggcgcat
     9241 ccatttttct ttttctactg catcaagatt tgtatttacc aattaccctt ccctgccttt
     9301 gttttttctt gttttacttt agcatatgat aaaaaggctg taactaactt ttttttctat
     9361 ggtaaaataa atacaagagg tgaacaagat gaaaatggat aaattacttg ctctttaccc
     9421 acaagcacag aaaaaaaact ctccagcatc agaagcggat atcctttctt ttgcaattga
     9481 tgatgctttt atctggatca aacaagattc attatctcaa caagaaacca gccttttgaa
     9541 agcgttattc cctgtaatca acgatcaaaa aaaacaccca tggtatcact atctttttaa
     9601 agatgcttct tgtgttcaag aaaaaagctt tcgtttgatc cagcttcacg tcgaatcaaa
     9661 aggagaattc ttaaaaaaag aatggcaaga tactattttg gaattatttc gctattctgc
     9721 tgatttcttt ttttatacag acacagatgc gctcttagtc gaacaaataa accgtgatta
     9781 tcttgatgct actgaaatca acggtatttt tctatcttta gatgctgatt ttgatacgac
     9841 aacgtctgta ttcgttggtt ctttccattc acctggcacc gacctcgtcc ctttgtttgc
     9901 tgaagaaaga aaaatttttt taacagagca aaacaatctt tatactcaca gccgtacttt
     9961 ttctatgcaa gatgtggctt tacattacta tacaaaagat actgtaaaag atagtaagtt
    10021 gatcaaaagt tacatagaac tgatcaaaca aaatgaaatg gaagtgatca ttcaagaatt
    10081 atggaatgaa ttaggcaatg tcagctctgc tgcaaaaaca ctgtttcttc atcgtaatac
    10141 tctaaaatat cgaatcgaaa aatttcaaga acagtctggt ttcaatctaa aaaaagccaa
    10201 cgatttactg ttttgctatc ttttattgct tcatgaacat aattaaagat agattaaagg
    10261 ggttgtgaca ataattttgt cacaacccct tatttcgaat gaatagtgtt caaaagctag
    10321 tttctcttac atcatttttt caacaaaatg cattagccgg tcttttacat cttcggttga
    10381 atctttgatt gaatcaccat aaacagagat tttctttttt cctgatttga tttttgctaa
    10441 cattcccatc aaagaatcca agtcactatc tgaaagacca tctacgattt ctagtaattt
    10501 ttcatttcca tcaaatttgt catagacgaa tttttttact ttcgtccgag tagtcatatg
    10561 atggatcttt tccattactt ttcccgatac aacgactgct gtcgctacac tggcagcggc
    10621 tgctacgctt aatccgattg ttacttttgt tgatgttttc ataagtatca gctccttttt
    10681 atctatctcc atcatagcat agacaaaaaa ataaacgaac taatccactc agtgaaatct
    10741 caatccttta gggaaaaagt tctttactgt tccttttact tgtttcccaa atccgacagg
    10801 tatcttatct aatactagca aaacccatcc ttgattccca tcacgttgaa atgtttctcc
    10861 tgctgtgtaa gattgccatt ccttttgagt gatcggcaag caaggtatat tctctatctt
    10921 ttttgtagca agcgctaatg catagctagg ttcaaatcga ttctttttga aatctcctaa
    10981 atgcaaacct gtacgtacga cttttaaacc atcgagagaa ggggctaact caggaacttc
    11041 ccaaagatgg tcgttgaaca ctaaaagtct tccagtagct tcataatgaa aatcatttga
    11101 aaactccgtc cagagttttt cctgctcttt tgtcatttgc actttacttt tttttcgtgt
    11161 cttcttttct ttatgcatct gattttgtcc atgaaaagtc agctttgcaa caaaatgtcc
    11221 ttcaccctga tctttatgag gccatatacg tattgttttt tccaaaccag ctacgcttcc
    11281 ccattcagaa cgtcctgaac taacggattg agtcaaagga atctcctcaa tcgttactgg
    11341 ataattttct acaagccagc taatgatctc ttcattttct tctggtgcaa acgtacaagt
    11401 cgaataaatc agttgtcctt tattttttag catttttatt gctgaagaca aaatctcttg
    11461 ctgacgtttt tgacagtata gcggagattc ttctgtccac tcttttattg catttggatc
    11521 ttttcggaac attccttctc ctgaacaagg agcatcaacc acgatccgat caaaaaagcc
    11581 cgaaaaatga gggacaagtt ctgcgggtgc atgatttgtc actattgcgt tcgaaactcc
    11641 ccatcgttct atattttctg ataaaatctt cgcacgtttc ggaaagatct cattcgtcac
    11701 taataaccct ttccccttca tttgagcagc taattgggtt gatttaccac cgggagccgc
    11761 acacaaatcc aaaacttttt caccgggttt tgctgcagca gctgttccaa cgatcattgc
    11821 acttggttct tggctatatt catacccagc ttgatggagg aaagactttc cattgacagt
    11881 gccaagaaat ccctcattgc tgtagggagc gggttgtaat tcttcgctat ggtaagtttg
    11941 gaccatgtcc aatcctgccg gttttagtgg attccaccga aatccttttt tcacgcttcc
    12001 ttgttcaagc gcagaaaaaa aatcggatgc ttcttcgcct aaaagaagac gatatttttt
    12061 gataaattgc tgtggtaacg ttgtcgcttc ctccttcatt ccaatgtttc gttagacatt
    12121 ttacaatctt ttttacgaaa aataaaggtg gctgatctac tttttcatgt tgctgcctaa
    12181 ggtgactgtt cctttaagat attttccaag tattcatccg tcacctcgtt tttatcccaa
    12241 acagcgttca tcatttcaag aaaatcttga gaagaatcat ctgagcgcaa acttcccaat
    12301 gcattaagcc ataccaattc aagcgacttt tcttctttga tcttcgtgtg acctttgaca
    12361 tatgagaaaa atggccgctt aaatagcgaa tccatgtcta aatcagattt tgcaatgatt
    12421 tcacgaatac tttcatggat agaaaaaaaa tctttcaata agtagacaat gcttaaaaca
    12481 tcatcggaac tgcattcagc taagtagttt tcaaaataaa cagtgatttc ttctaatatc
    12541 ttctctttta acccgctgat tgatttgaaa tgtgtataaa tcgggtatgt agaacaaccg
    12601 cagaactctg ctaactctct tgcagtaatt gacttcattc catattcttc cacgtatacc
    12661 atacttttga acagcaaata ttctttacta taatttgtta ctctccccat cattcttacc
    12721 tccgtctctt ttcatctacc cataaataaa taacataaat aattctaaaa atattattga
    12781 taattatcaa aacatattta gttatatcca ttaaaaatca atttttccgc cttaaaaaca
    12841 agaatatctt gacaaaatac cctaggaaat aaacaataga ataaacaagg taacaaaaaa
    12901 attaaataac gttatataaa aaagaaggcg acactctgtg agaataacac agactgaaat
    12961 gactttccac tcacagtttt tgtctaacta tatacaaaaa cagaataaaa aggaggtatt
    13021 tatgctaatt gaagcaagaa aaaaagagtt acttgttcta caaatggtca ttcaaaaaaa
    13081 taattgttct ttacaagaat tatccaatga cttacatatt ccaaaacgga caatcaaaga
    13141 actgatccga aaaataaatg tatcactcaa gcaattttta ttgattgatg aatttattta
    13201 ttctaatcat aaaggagaga tccaaaccaa agaaaccgat caaactaaaa agttagctgt
    13261 tttttctaag ttaaaattat gctacctaaa agaatccaat cgttttaact atttgatgtt
    13321 actaatcaat tatccacaaa ctgctgtacc aaaaaaatat ttacttgaac aactttatat
    13381 atcgccctcc tatctcgaaa aactgacaaa acagctcaat cattccttaa aaaaattcca
    13441 tttacggatc ctatctgtaa aaaattgctg tactcttgaa ggaaatgagt tatctatccg
    13501 tctctatctt tacttttttc tttcagatac atttggaggt aacgattggc cattttcctc
    13561 acttgaatta aatgcactaa aaatgaacag tacagatgag caaatagaaa aaatttcaaa
    13621 acaagaaaaa aatatctact tgctgttaac tttattcata ttacgaaaca aacaacaggc
    13681 atattttcca aaaacttctt ctgaaatcac tgaattgatg gacttattgc agcatacaca
    13741 tgattattca gaatacttta cttctttttc agtgagtcgc ccttgtgaag agacagaaac
    13801 agagagagcc tactttaatt attttttcta ttgttttttt ccaaatcaaa tctcttttcg
    13861 acaaaaacag gagattggtt ttttattttc cacaagcaaa aaccttattt gtcgacaagt
    13921 cggtcaattg atgacttact taactcaaca gtttcctctc aaccatttag aaaataaaat
    13981 accaatttat ttttattttc ttgtgttggc agttgttttt ttcgaactgt tgggtgaatc
    14041 cagcagtagt ttttctacac tttgctttcc agaagccacg tccaatcatt ctagttcttt
    14101 tatacaggaa aagaaattaa gagaaaaaat cgatacaatt ttttcaaaaa atggggaaag
    14161 aaaccaaatc agtcaatacc ttagtcaatt gatccaacac ttactacaaa cagaaacgaa
    14221 agagcagtta ctcatccatt tgcagataac caaacagttt acaggaaatt atcatcttca
    14281 aagccatttg cagcagttat ttcgcaaaga tgctgttaaa attaccgcat attcagaaca
    14341 tgctgatttg atcattacag atcagcatga gaaaatttca gatgaaacgg agactttcta
    14401 tctggataca acgcacaata cagtgttatt tggcaagtta ataaaagcgg ttcatgaact
    14461 gtatatgaaa aaaggttatg agaacaaaac aaagaggatg tgacaaaatt cttgtcacat
    14521 cctcttattc caaataaatg gtattcaatc agctaactct cggtattagc tcagatcaaa
    14581 gcgctgattt cacaacttct ttattttaat ctcttcggtt gccgccgaac aataagatca
    14641 agcgcaacag ctgcaggaat gtcgaaagcg ctgctgcaac ataagtcagt gctgctgcaa
    14701 ataatacgtg tcttgccatc ggtacttctt cttccgtcag tagtccgcct tcactcaaaa
    14761 tttggattgc tcgccgagac gcgttaaatt cgactggtaa tgtcaccaat tggaaaagta
    14821 atgctaaaga aaaagcgaaa atcccgatat tgattaacgt agagttccag ctaagcaata
    14881 cacctaccat aatcaagggg aacgaaatca tcgaaccaaa gttagctgct ggaacaagtg
    14941 ctgcacgaat cttcaatggg acataacccg aagcatcttg taccgcatgt ccgcattcat
    15001 gagctgctac acctatcgca gcaactgaag tagactgtgc agttgcttga gacaaactca
    15061 acatcttatt acctgaattg tagttatctg tgagatctcc ggcaatttgt tgaacaccga
    15121 cattatttat ctgttgactt tgtaatatat attgtgctgc ttgtgtacca gtcacattgt
    15181 ttttgcttct cacttgatcg tattttcgga aagtactatt gacgtacgct gacgcaattc
    15241 cggaaataac taatccaaga atgaccaaaa gaaatgtcgg atctaaaaaa ccatacattc
    15301 cataaccata cattttcgtt tcactccttt tcgtttactt tattttatca ttagtagtag
    15361 gaaaagaatg tgaaaagacc ttgaatattc tttaattctt tttcacaaat tccgatttca
    15421 gtttcatcgg tccaaatcct tctaccttgc aatcgatatt atgatcgcct tctactaaac
    15481 ggatgttctt gacttttgtt ccttgtttaa tggcacctga cgcacctttt actttcaagt
    15541 ctttaatcac tgtcacgcta tcgccctcta acagacggtt tccatttgca tctaatacca
    15601 ctaatccgtc ttctatgaca gtttcttgtt cttctttgtt ccattcgttc ccgcattctg
    15661 ggcaaatcaa caagcctcga tcttcatatg tgtactctga gccacattct gggcaatttg
    15721 gtaatgtcat cttctttctc ctttaaaatc tttagtgagc actattgtaa cggaaaaaga
    15781 agatcttgaa aagatacatt tgtgattttt actacaaaag gcacgttttc gaacgaagta
    15841 tcattctgtt tttctgctct tatacgattg gcatatatct tggatctctt tatagatttc
    15901 ctctacttct gctaaatctt ttgcattcgc accacttgct aatggatggc ctcctccatg
    15961 atggcgttta gccaattcat tgatgatcgg ccccttagaa cgtaaacgta cgcggtaata
    16021 ccctgaaggc tgctgaacaa aaatccccca agccagcact tcgtcaatca ctccaggaag
    16081 tgaaacgatc gaagctgtct ctgaatcatc gattccatat ttatcaagaa tttcttgcgg
    16141 aaggattatt cttcctgcac cattttcatc cacctggata ttttgataaa gataaccgga
    16201 aagtttagcg actttcatcg gcatttgttc aatctctctg ttcaaagcac ttgcatcaaa
    16261 tggataattt ctcaatatcg aagcaatctc taatgtacgg gacgtcgtgg agggatacaa
    16321 aaagcgccct gtatctccga cgattcctgc ataaagtaac cgtgcagcct taacgttcat
    16381 ctttaaagat tcaggaaaca tcaaagcaaa ttctgcaatc atctcactac agctacttgc
    16441 ttttgtattt acccacacaa gatctccata agggtcatca ttgggatgat ggtcgatttt
    16501 gatcagtttt tctgccaact gatatctttg atcactgatt ctgggtgaat tagctgtatc
    16561 agtcacgatg actagcgctc cttggtagaa gacatcttca acttcttgca tttctgctaa
    16621 aaagcgaagc ccttcaatcg tttcacctac ttgacgtatt tctttttctg ggaaactttc
    16681 ccgtaatagt tcggctaatc ctacttgtga tcctaatgca tcaggatcag ggcgcaaatg
    16741 cctgtggata atgattttat cgtatttttt tattgtatct actatttctt gctgtactgt
    16801 catttcactc ctgctttcta agaacgttcc attacttgac aaacaacgat tgctttggaa
    16861 acgatggtgt tttcaataaa cacttcgata tccagtttag cggaacgtct gccgatttcc
    16921 aatacacgtg gacgaatgtc taattcgctt tccaattgga tcagccgcaa ataatgtagg
    16981 ttcacttgtt caatcaagat atttcgtttt tggttcatca ccattgtttt ttgtgcgaca
    17041 tcagaaatga tttcgctaag tacgccaaaa gaaatggttc caacgccatt gaccatttgt
    17101 ggtgctacta cgaatttgaa cttagggttt tccaaacgat ttccttcacg gtcagtatca
    17161 atcgtatgga tatttccaga tatctgatcc gaaatcgtat ctgcaatctg cggttgtcgc
    17221 tggacaagct gcatcgcttt cattacatct tgccgggtaa tcaagccttc caatgtcaag
    17281 tcatcggaaa cgaccggcat gacttctagc ccatcccaga tcatttgatg gctaacagag
    17341 gcaatactca ttgttttctt tacactgatt ggttctttcg tcatcacccg atcgatgatc
    17401 tggtttggcg atttccctaa aacatctttt gccgtaacaa tccccatcaa acgatgatgg
    17461 cggttgacga ctggaaaacg cgaatgctgc gtttcttctg acaaacgctg ataatcagaa
    17521 atcgtatctg tctgctgcaa ataatgagtc ttttcagctg gcatatagat atcactgaca
    17581 agcatgatat cttttttgat caattggtcg cttaacgctc ggttgatcat ggttgcgact
    17641 gtaaatgtat cataagaggt gcgtaagatc ggcatttcaa gatcatcagc tagctttgca
    17701 atctcagcag tcgtattgaa tcctcctgtg atcaatactg cggcgccatc ttccaatgct
    17761 aatttttgga cttccgcccg gttgccgacg atcattagtg atcctggcgt aatatagcgt
    17821 tccatggcat gtttagtcat cgcgccgatg acgaatttat tcaagacctt gtctaatcct
    17881 gccgccccgc ctaaaacgtc cccttcaatg atcctgacga cctcgccaaa tgtcaaacgt
    17941 tcaatatgtt tttttaattt gcgttcgata cggatggtcc ctactcgttg aattgttgaa
    18001 actagtccga cattttctgc atctttgatt gcacggtacg ctgttccttc actgacattc
    18061 aattcttttg cgatgcttct tacagaaatt cgattgccaa caggtaaact ttcaatatat
    18121 tctaaaatca aatcatgttt tgtcgccatt ttctgcctac acttccagcg tttccgagac
    18181 gctcattatt tttccttcac tatcttccag taactctaca aaagattcgg gatcttgttc
    18241 aatgacaggg aaagtattgt aatggattgg aactactttc ttggcacgaa ttgtttgcgc
    18301 agcctttgct gcatcttctg gccccatcgt atagttatcg ccaatcggca agaaagcgac
    18361 atcaatatcg aattcttctg caagcaatcc tagatcactg aaataagcag tatcccctgc
    18421 atgataaatc gtttttcctt ctgcttgaat aataattccg ctaggctcgc ccatatactg
    18481 aacgataccg tctacttcat agcttgaact atgctgtgca tgtacgaatt ttaccacgcc
    18541 gaatggaaac gcatgttttc caccgatatt cataccgaat gaatctactc ctctgctgtt
    18601 cgcataatca gcaatctcaa caatactgat aattgtggct tgattttctt ttgcaatagg
    18661 aatcatatct cctatgtggt cactatgacc atgagtaatc agcaaccaat ctgtctttac
    18721 ttcttctgca gttaagtctg tcaatggatt tcctgtaata aacgggtcaa tcatcacgcg
    18781 ttggccattt tccatttcaa ttgttaaaca ggaatgtcca tgatacgtaa ttttcatatt
    18841 ttacacctcg cgttttctct gtatcttttt tattaaaaac gatttcatca cttgcacatc
    18901 aatgaaaaaa tatagctgct aaatatattt tcattggatt gatagtacag tatgtacaaa
    18961 gcaattgtaa cagataatac aggatgcacc aagtaccaag cagatataat ctcaagtttg
    19021 aggtctctgc tctgcttagc ttctcttctc gtcccttaac taattaaatc agcgtaaata
    19081 ggatggacga tcttttctaa atcggctact tttatccgaa ggcttctccc gatttcaccg
    19141 cttgacacaa ttacttcttc tttttctttt aaagaactgt ctgctaaaaa acaataatca
    19201 ggatgtgcga gttttattcc aataggggtg tttgctccgt ggggatatcc tgtatgcatt
    19261 aatacaaaat ccattccagg aaaaccaact ttacgatctt tcgtcacttg tctcgttttt
    19321 ttgtaatcta agtgatcgtt caaaggaatc aatgcgatga tcaccccagt tttgtttcct
    19381 ttcactacga gagttttaca tatcaaagct ggatcaatcc ctcttttttg tagttcttct
    19441 gtgaaagatt cttctaccac atgactaaaa tcaagtggtt gatagctgat cttttgttct
    19501 tctaaataac gttcgacttt tgtttgactc atcattgatt cactcccttt attctatcgt
    19561 ttattataaa gcgagggatg ctgtattcaa aaattatttg tttttcgcaa aaaaagagca
    19621 agacgagcac tctatagaga ggttcatctt actctttttg tttatttttt atttcgacgt
    19681 tgagtagctt ccataatgac tggcaagatc actgggcggc gttttgtttg ttcaaataag
    19741 taacggccta attgatctcg gatgtcttgc tttaatttgc tccactcaaa atcattgctt
    19801 tctaaatgtt tctcaacgat tgtttcaatc atattgctgc tttctttcat cagatcacgg
    19861 ctagctttta cgtaaacgaa tccccgtgaa gtaatttgag gttttgaaat gattttcttt
    19921 tctcgccgac tgatagtgac aaccgctacg aaaatcccgt cttctgacaa gatttttcga
    19981 tcacgtaaaa caatgtttcc gatatcacct atgcctaatc catcaatcat tacattttct
    20041 gcacttgtag ttcctgcaac actcatacgc ttgttcttat attccagcac gtctccgcgt
    20101 ccagtaatat agatattttt gtatggcatc cctactgcat gagccaaatc tgcatgagca
    20161 gctaattcac gatactctcc ttgaactggc acaaaatatg ttggtttcat caaattgatc
    20221 attaattgca agtcattagg attggcatgg ccagaaacgc gcatattctc agagatcaac
    20281 ttcactgttc caccagctcg ataaatgata tcttccgttt tcgccacgat ggtttccatc
    20341 gcgatgcttg gcgtagtagt gatataaacc aaatctcctt cttcaatctt gacattacgg
    20401 tgtgtaccgt ttgccatttt ttgcagagac ttgatgggtt cacccattcg gccagtttct
    20461 aaaatgatca gttcttctgg atcatagttt ttcatgtctt tcaacgtaac aagcagatct
    20521 tcacttggta attgaagttt ttctaaacgc atcgctgtgc gaataattcg ttcgaaatct
    20581 tgaccagtca agacaacttt tcgttgtgct ttttctgctg catccaatac ttgttgcact
    20641 cgttgcaaat tacttgctac acaagcgacg atgatacgcc cttcccaata tttgatcgta
    20701 tcaaatactt catctgctat ttgggcttca gaagctacag gctgaggatt ttctgcattt
    20761 gccgattcac ttagtaaggc taagactcct tctttgccga tttccgccaa tcgtgcataa
    20821 tctgtttgat acattgggat agccgtttgg tcaaatttaa aatctcccgt ataaacgata
    20881 ctaccttctt ttgttttcag attgatcccc actgaatctg gtatcgtatg agtggtacgg
    20941 aaaaagctca ctactgtttc accaaagtct atttctgtat gctcatctac cacatggaaa
    21001 tccttaaatc ctttggctgc ttcattacga ctgacattca attttgctaa ctcgatcgtc
    21061 aactctgtcc cgaatactgg tacagaaatt tttgacaaca agtaaggaag tgccccgatc
    21121 gcatctgcat gtccatgtgt taaaaagaca ccagctacac gatcaatgtt ttcttctaaa
    21181 tacgtaaaat cagggattac gacgtcgatc cccaacaact cattttcagg atattttagc
    21241 ccacaatcta aaataaaaat atcctcgccc acttctgcga catacatgtt ttttccattt
    21301 tcacgaacgc cgcctaatgg gataatttta atggtactca caaacttcac ctcttcttat
    21361 gacatccaag tttaaagatg tcgattttct tctttatcta aatatatgag ccattccaat
    21421 gaagcatatg caaaatcact atttctcaac ttatggagaa tcagcaggaa aattaccagc
    21481 acatccgcgt tctgacagtt ggatgggcat tttattttgg atatacttca ttaacaaaat
    21541 ggatcttttg cgaaacgcta tagccagaat acttatataa gtattcagct gttccccatt
    21601 attatacaac aaataaacta aatcgctagt atcagcaatt gttagaacaa ctatgcacgt
    21661 aaaaatccct ctgtagcatc taggatttct tttgctattc ctctttcatt tcttcttcgt
    21721 cggtttgaaa catacttttt tcattttacc acattttttg caataactat agtgactttt
    21781 tatatataca gaacctcatg ttgatggtgt gcaaacagca taagatttaa gctagcttta
    21841 agataggttg tctattatca aatgcatcta ctaggaggga tgcaaaatat gaattttatg
    21901 aaacgtgcat ggaaaagtac aaaagtaaaa tggggacgtt cattgttatt gtttgccgtg
    21961 ttcacagcca ttcttatttt tgtgctagct ggcctcacga ttcgaagcgc tgcagaagaa
    22021 gcagcgaatc aagcacaaaa agaagttgga gcaacagtga ctttaagtgc caatcgagaa
    22081 gcttcattta aaaaacagga acaaacttct tcaagcggtt ctaccactga agaacgtcca
    22141 gatccaggca gtttttctct tacaccagtt tccttaacgg acgcggaaaa aatagctgaa
    22201 ttagataatg taaagagtta ttcctttgaa agttccgcct cagctatggc aaaagatgga
    22261 ataactgcca tcactagttc tgattcaagt gaagacagtt cagaaactac tgatgcaggc
    22321 ggtgaacctg ctggaggaat gggcgggaat ccgcaaatga ctcaagcaga ctttcaaatc
    22381 agtggtgtgt cagcaactgc acaaaatagt aatttttctg atggaacagc aaaaatcacg
    22441 gatggtgaag gaatagatga cagcgacaaa ggcacaaaca atgtagtcat tgattctact
    22501 ctagcagaaa caaatgacct ggcagtcgga gatactttca caatcacgag tactgaagat
    22561 gaagatacca cttatgaaat gacgatcaaa ggaatttatg aatccagcga aacaagcggc
    22621 agtatgggta tgaaattcaa cttcatgaat ccgtcgaata cactttacac ttcttacact
    22681 tttgcaaatg aactaaatgg gacatctgac gataacacga tagattctgc tatttacact
    22741 ctatctgatc cagataaaat ggatgagttt ctcaaagaag ctgagaagct gatcgacaca
    22801 gatacattta gcttacaatc aaatgattcc atgtatcagt caatgttgga acctttaaac
    22861 aacgtggcaa gtttttctaa aaatgttgtt ttactagtgg ctgtagccgg tattatcata
    22921 cttactctga tcattatgat taccattcgg gagcgtcggc atgagttagg cgtcttgcta
    22981 tctttaggag aatccagaag taaaatcatt ctccaattgt ttaccgaagt ggctatttgt
    23041 atgattcttg cattaggagt tgcttcgctt agcggaaacg tcgtcgcgaa tgccgtggga
    23101 cagcagctac ttgaccaaca gacagaaacc acgacacaaa accagcaagc tcctggcggt
    23161 ggacaaatgc caggtaacag acaagaacaa ggcggtcaac gcgatggtca acaaggaacc
    23221 acgaataatc catttgctgt ttctgatcaa gtcagcgaat tggaaatcaa tgtcaaacct
    23281 gcacaattgg gactattagc cggagtagca tttggaatca gtctgttttc tgtcttttta
    23341 gcttctatag ggatattaag attaaaccca agaaaaatcc tattaaacta aaggaggaat
    23401 ttgatgttac ggacagaaca attgggattt tggtatcaag atccgcaaga tgcgctttat
    23461 aaagatgtag aactgaattt cgaagctgga aaaatgtacg ctattttagg agcaagtggt
    23521 tctggaaaaa cgacttttct ttcgcttatt tctggactag atgcaccaaa agaaggaaag
    23581 gtatactatc aagatcgttc actgaacaaa atcgggttaa gaagttatcg taaaaatgat
    23641 gtctcgatta ttttccaagc ttacaacttg cttccttatt tatcggctct agacaacgtc
    23701 atagcagcaa tggaaatcac taagaccaag caagagaatt atcgacaagt tgctttagat
    23761 cgactggctc gtgtaggaat tgatgaaata ttagcaaaaa aacaggtgac caaactttcg
    23821 ggaggtcagc agcagcgagt cgctatcgtt cgtgctatct gctgtgaaca tcaactaatc
    23881 gtagcggatg agcctactgg aaatctagat gaaacaactt cacaagatat cgtcaaactt
    23941 ttccaagaaa tcgcccatga acaaaatcgc tgtattatca tcgtgactca tgagattgaa
    24001 gtagcaaaaa gctgtgatga agtctttgaa ctaaaaaata aaaaatttca aaaaatcgaa
    24061 gtttaaaaaa caggataatg atcaaataaa aaatccgaac catatctgtc tcgcagatat
    24121 ggttcggatt ttttatttgc ttgattcaat tagtaataag tggtttttat tttttttcgt
    24181 cttttcgaga aaacttgtct ttgacttcgt cgcctaaatt tcgagctttt tctttgatgt
    24241 cagcacctag tgattgtgct ttcccttctg tttctttgct cttgtcgtct gttgctttgc
    24301 cgtatgcttc ttttccttca ccgactactt tatctttcat atctttagaa cgtcctttta
    24361 aatctgccat tgtgcttcct cctcttagtt tgtttattga tctaccttca gggtaacatc
    24421 aacaacagat acagacaaac agaacgctcg ttttctaaaa aagatagtta gagctgttga
    24481 ttaaaaactt ttttcgaaag tcccccatac tttgacttct ttttcttgga ccattatttc
    24541 tcctttagcg ataactgtat caatatcaag cgtttcttta tccaagatac acagatctgc
    24601 atcagcacca atttcgattt ttcctttttt atccaatttc aatatacgtg ctggattact
    24661 ggttatagct cgtaaggcga tttccaaagg gatactttct ttttgcacag cttctttgat
    24721 ccctactagc aatgccttgg cacttcctac tcctaaccct aggaaatgat tgttttcatc
    24781 aaaataaggc aagcttcctt gtccatctga ggtcatcgtg aaattatcta aagaaacatc
    24841 ttcctcgatc aatcgtttca gtgctttgct gaagcgaact tcaccatctg tcttctccca
    24901 aaaatcagga tcttcgctag cagtgaagtc aatcgtgcca ccacgtttgg caaatgccac
    24961 gcaagcttca aataattctg gtgtgcgatt tgcatgagta ggcaaaaatt gtgtgatcgg
    25021 aatttctgtt tcttctacga ttcgtgtcaa tagctctagc tttctttttc ctccgcctaa
    25081 gtggacattg atgatcccag cttttccgga taacatgcct cctacacgag catctgctac
    25141 tgcacgagcg aactcttcaa aacttggctg agaagaacgg tgatcggata ctgcgatttc
    25201 tccgattccg ataattttat caattgtcaa aaagtctttg atgatagaat ctgttactgt
    25261 ttttaccggc aatcggtacg aaccttcgta tacataagtg gagattcctt ctgcttccag
    25321 acctttcgct ttgctgatca acgcagtcat atctctacct tctccgtctg ttcccaaaca
    25381 accgacaact gtcgttacac cagctgtagt caattggctc aatgtgactt ctggcgttcg
    25441 attttgataa ccgccttctc ctcctccacc taaaatatgg aaatgacagt caatgaaacc
    25501 cggggttagt acttttccct cacctgaaag ttcttctact tccactcctt cgtagccgcc
    25561 ttctagctgt tcttctacag ccaaaatctt cccaccggca atcagtacat ccatttttcc
    25621 taagtaatct ggtgcaaaca cttcaatttg tcgaatgatt ttcatacatt tttcctcttt
    25681 ctttaagtat aactgatgat aggatcttaa aaaataatcc aaacgatagg atctttactc
    25741 ctttgatttt accgtaaaat gacggttctt ggagtcctta tctactgttt ggattacttt
    25801 tttatttaca ggtctccagt actactgcag acctattgca atggcaatca tgatgaaaat
    25861 agttcctaaa acaaacagga atccttgcat cttgatttgg aatcttgccc attttgccca
    25921 ttcgatccgt gcaacaccta gcacaccaat caagctggct gatacgggtg taaaagcgtc
    25981 cataaaacct gaaccaagct gataagcaag aactgctact tgtctggata cacctactaa
    26041 atctgccagt ggagccatga ttggcatagt caaagctgct tgtcctgaat ttgatgtaac
    26101 aaccaagttg aacaaacttt ggaagatgta catcgcccaa gcaccaatga cagctggaac
    26161 accggtcaac gccgtcccga tcccatgcaa aatcgtattc aaagctgaag gaacagatgc
    26221 atcagatcct cccaagacaa gaagaatccc tttggccata cctactacga ttgctgtacc
    26281 agctaaatca gctgcaccag attggaaaga agaagcgatg tcattgactt tcattccatc
    26341 taatttgaaa agaacagcaa tgattccagc aactaatccc atcacaaaga attgagaagc
    26401 gatttctgga atgtaatagc ctttttgagt cacgccccaa acgatccaga caaggacaac
    26461 taacatctct aataagatca atttatgccc aagcatgaat tttttatttt catcagatgt
    26521 ctttttgata tgagaacgga aataatcatc tgatttgtaa gtcaacgaac tttctggttt
    26581 tttacggatt ttttctgcat atatcatcat ataaccagcg gctaatgcag taacaactac
    26641 ccacataatc aatcggaatg tcgcaccaga caacacgggg attccagcaa tcccttgagc
    26701 caccgctacg ctaaacgggc tcatccaaga ggtcgcattt ccgacttgag atgcgacata
    26761 agtcacggtt actgctacga tcgagtcata tcctaacgca atgacaaatg gaaccatcac
    26821 cattgagaat ggaattacct cttctgccat cccgaatgta gcgccaccga atgaaaaagc
    26881 aaagaataat agcggtaaag ctaaacgctc cagtccctta gttttactga taaacgcata
    26941 gatacctgcg tcgatcgctc cggtacgcat aataatacca aaagcacctc caacaacaag
    27001 gatcaatgca gcgatcccta cagcagatcc atacttatct ccagatacta gtccttcaaa
    27061 aacaaaattt aagaaaccaa agccgccaaa atcatctgtt ccccatattt tggctgtttt
    27121 atgcaatttt ttagaagtat catagatatt ttcgccgtac tgatcgtaaa gcacatcatc
    27181 tgtcaaggag atttcatcta gtttttcctg cgtaagattt ttttctccgt cagcaatgac
    27241 tttttccaat cctttttccg gaacatccag tttttcacgt tctgctggat gatcttgcag
    27301 ttcttctaat tgatcaaaca catacgactt atccagctcg taagcataac ggaaggaatc
    27361 ttgacgtaaa acagtacgag tacttgtttc gccatttgca tctttgtatt caatatcttg
    27421 cgtgctgaac tttcctgctg gaacgacaaa agtcagtatc catgcaaaga tgactacacc
    27481 aaaaataatg acatacgtat ggggtgtcga taatttagtg aagtcgcgtt tcggttttcc
    27541 tgaagaattt gttttcttca aatttatttc ttcctttctt ttttcagtaa ttttgatgaa
    27601 taaaaaatgg ataatcaaat gaattataca gtaaaaatga catttccaca tgaataattt
    27661 attagaaaat cacttttttc tattttgttt tttttctcct atgtatgatt atttttttct
    27721 ctttccattt atattaagta ttttatttct ctgtttttcc ttaaaattct ttttttattt
    27781 ttagattaga gaaaatctcg tcctgttttt ttcattttac aatcattgaa aaattgaatt
    27841 tcattgcaaa aaaaataata aacacttata aatatccatt atctatcctt caatttttgt
    27901 gtattttatt tctattagaa aaaggtatcc tgttggacaa ttttgttata tcaggtaaac
    27961 ttcattgcaa taatgataac aaatgtacta taattacttt gtaaagagtt ttggggaaga
    28021 ctctttcatc aacgtgtatt aagagacaag aaagaatcca tatggattct ttcttgtctc
    28081 tttttccact actttttatt tttctgacac tacatattct tataactcat ttctcataac
    28141 tgtgaaacaa cttataaaaa taacaaactt taaacaaaac aagcttaaaa tcaaagatga
    28201 tccatcttaa aataactaaa acaaaaaaag tagtcgttgt taatcctcat taataatcgt
    28261 tttctttttt tcaaacgctc catttttatg tggaacatta tttttttaag gaactttgtc
    28321 aacatgtgga taactatttt ccacttgtgt cctggactag ctgttttgct gtaaaataca
    28381 aaacttgctg cagcaaaaag atcgttgcaa gagaggctgt tccgcagatc gctggatcta
    28441 gatttggcaa agtcaagact gcatcatgta aattggcgtg gttgtccaaa accaaatcag
    28501 caaattcaaa taatcgcaaa ccactgctat gttgcgattt gatacttcgt gacaaatcaa
    28561 atccagtaac aatgattaat ggatgaccat tttctttgat ccactgagct agttcgatga
    28621 ttgccgggtt tcttccttca ttcgaaagaa gaagaaaaat gtcttctgat ttgacagaaa
    28681 gctgatcaat aattgcctgt cctgcacctt ctagcgtttc atagacacct ccggctggat
    28741 tttcaatcaa tatacttggt acaagatccg gggtttgatc ccaaaattca aatgctacac
    28801 cacttagtgt gcggctagca tatatctgca atctaccacc ttgattaatt tgtttcgcga
    28861 tccaagtgcc agcttgaagc atcagcggct cttctatcct taatttctct tccaattggt
    28921 gataatagtc aaaataacat aaactcattt atctctcccg cctagctata cttgacatga
    28981 atagaaacag ctgcctaatc taaggaagta cataaaaaaa cagtagttag acagtaacac
    29041 atgtggtctt tgctgtttca aacacaaaaa aggcattgaa cgaatacgcc caataccttg
    29101 acatagcaaa taaaacaacg tgcagaccat ggaaaatatc tacatagggg tagaattttc
    29161 accctagatt aggtacaata aattaggtcg atgcactgca ttgactatgg caaaatgatc
    29221 ggtcagggtg tatgtgtgtg actggtcatt ccctgattct tagcgccaac taagggtcag
    29281 ggagcctttt tttattcagc tgtttctatc tgaatttatt gtaagtaatc tcttatccaa
    29341 gtgcaagcaa gaaaatacaa aaaaacattg gtatgattta tatatatata aataacataa
    29401 aaaaaggaat ggcttatccc gatacaacaa gcattccttt aaaaaagatt cttttatata
    29461 agtgaaaaaa gcttttttcc accaattaaa taaatctctc atctcaatag tttatccatt
    29521 attttatctt tttttgcgta tacctactaa atattaccgt cttcttctcg ttcttctact
    29581 ttgttcgaga gaatctgtac tagaaaaagt agttgtatgt ctaccttttc tgcctatccg
    29641 agactttctt ttcatatatg ggatataaat gattagatac agtacagcaa aaaataaagc
    29701 aaatacgcaa gcttcccaac tacctaacgc caattcaacc atcaaggtag caaccccaac
    29761 ggatgctgca taccagataa aaatcagaaa cgtttctttt tttctgacca tgcgaaacat
    29821 gataaataga ctaataagca atagtggcaa cacaaatatc caattgcttc cttccatcag
    29881 ccacttgagt atatcaagca gcgactgtgc aacagcgact aattttccca tagtgaacac
    29941 acaccttttt ttataattaa aaaattcgct tttttattct actatgaatc ttctggcttg
    30001 gctttcattt ctcttatgta atcattttct tttatacaac ttaaaaaaaa attaaaccat
    30061 tagcataata ttttctgaat ctattcacga aagtcttttt ctatctttcg ttttccacga
    30121 ctcaactgat ctagagtcaa aaaactaaaa cgacagttgc gcaaatgacg cacaagctgc
    30181 cgctttaata acaaatagcc tttcattctc agcaggccac ttatttccag ttaactttct
    30241 gttcttagct ttttactcaa ctggataata tatgcactta atgaagtccc aatttccgaa
    30301 tgttgaagtc catattcaat atttgtcttc atatatccta acttatcgcc tacgtcgtat
    30361 ctttttcctt taaactctct cgcatagacc gcctctgtct ggttaagagt ctgaagtgca
    30421 tccgttagct gtatctcgtt tcctgctcct ggttcttgag tttctaatat ctcaaatatt
    30481 ttaggagtca gtagataacg tccgataatg gctaaattac ttggtgcttt tcccatttca
    30541 ggtttttcta caaacccttt tactctgcaa cgaccctttt cgctatctgt tttaggatca
    30601 ataattccgt atttagatgt ttcattttct ggtacacgca tgacggcaac agcggaagct
    30661 tgtgtttctt cataatccat catcaattgt tgagtcaatg ggatatcatc tttcatcaaa
    30721 tcatccccca gcatgacgac aaacggttca tttcctataa atgcttttgc ttgaagcacg
    30781 gcatccccca gtcctcttgg atgagattga cggatgaagt gaagattgac ttgagtcgtt
    30841 tcttcaacta aagcgagtaa ttctgttttt tccttttccc gcaaattgtt ttccaattcg
    30901 atatttgcat caaagtgatc ttcaatgggt cttttttctt ttcccgttac gatcaagatg
    30961 tcttcgattc ccgaatccaa tgcttcttcc acaataaact ggatcgttgg tttatccacg
    31021 attggaagca tttcttttgc catggctttt gttgccggca aaaaacgtgt tcctagccca
    31081 gcggcaggaa tgactgcttt tttgaccttc acttgct
//
LOCUS       DACKAC010000028        30587 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.028, whole genome shotgun sequence.
ACCESSION   DACKAC010000028 DACKAC010000000
VERSION     DACKAC010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.028"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..226
                     /locus_tag="I0B46_001340"
     CDS             <1..226
                     /locus_tag="I0B46_001340"
                     /inference="COORDINATES: protein motif:HMM:NF018513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAR2083651.1"
                     /translation="LWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKIIKEAIPSLLLI
                     VIIIGGILTGIFTAVEASAIAVIYKWVIRF"
     gene            208..513
                     /gene="kduI"
                     /locus_tag="I0B46_001341"
                     /pseudo
     CDS             208..513
                     /gene="kduI"
                     /locus_tag="I0B46_001341"
                     /inference="COORDINATES: protein motif:HMM:NF002091.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
     gene            533..1336
                     /locus_tag="I0B46_001342"
     CDS             533..1336
                     /locus_tag="I0B46_001342"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAR2083652.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIRDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(1585..2649)
                     /locus_tag="I0B46_001343"
     CDS             complement(1585..2649)
                     /locus_tag="I0B46_001343"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAR2083653.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKDPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAQEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(2646..3731)
                     /locus_tag="I0B46_001344"
     CDS             complement(2646..3731)
                     /locus_tag="I0B46_001344"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAR2083654.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(3744..4721)
                     /gene="mvaD"
                     /locus_tag="I0B46_001345"
     CDS             complement(3744..4721)
                     /gene="mvaD"
                     /locus_tag="I0B46_001345"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAR2083655.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKKGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(4714..5658)
                     /gene="mvk"
                     /locus_tag="I0B46_001346"
     CDS             complement(4714..5658)
                     /gene="mvk"
                     /locus_tag="I0B46_001346"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAR2083656.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5980..6633
                     /locus_tag="I0B46_001347"
     CDS             5980..6633
                     /locus_tag="I0B46_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAR2083657.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVSI"
     gene            6718..7623
                     /locus_tag="I0B46_001348"
     CDS             6718..7623
                     /locus_tag="I0B46_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAR2083658.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWEIALIISLVLSIWMLIALWRRIR"
     gene            complement(7625..8257)
                     /locus_tag="I0B46_001349"
     CDS             complement(7625..8257)
                     /locus_tag="I0B46_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAR2083659.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGNQSYLDAKN
                     MVLRESHIQKLTMQKTGLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTTIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            8577..9101
                     /gene="infC"
                     /locus_tag="I0B46_001350"
     CDS             8577..9101
                     /gene="infC"
                     /locus_tag="I0B46_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAR2083660.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            9173..9373
                     /gene="rpmI"
                     /locus_tag="I0B46_001351"
     CDS             9173..9373
                     /gene="rpmI"
                     /locus_tag="I0B46_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAR2083661.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            9426..9785
                     /gene="rplT"
                     /locus_tag="I0B46_001352"
     CDS             9426..9785
                     /gene="rplT"
                     /locus_tag="I0B46_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAR2083662.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            10137..11375
                     /locus_tag="I0B46_001353"
     CDS             10137..11375
                     /locus_tag="I0B46_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAR2083663.1"
                     /translation="MATVWMGLALVGNTFSPNSAMEGQGVIDVLTHIYTTGPAEYAKS
                     ILVNVFFGAFFGRVLVDSGIAATLIRKVVELGGDKPRITMSLLCIVTAVIFMSMTGIG
                     PVISIAVIVLPILMSLGISVPVALFSFMGSIMAGIFANIVNFKQYQTIYAGFNPAAES
                     YTYNDYFQIGMIGMIVSLVVVLTVANISMNKKKRYAMAANVPAEGGDAPMISWLAVLL
                     PVLGVVLLDLQIILGFILAGIWALLFTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGF
                     LMTLAMFNNSAAYASPYFSAIFGDWIPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSA
                     ILAVVLAVNPTMSPAFLFPLFAITTIAPQHLDITQSWVAWGLGYTKVTSREYMKKSIP
                     TGWIIGAILCLITFLLYGNA"
     gene            11423..13552
                     /locus_tag="I0B46_001354"
     CDS             11423..13552
                     /locus_tag="I0B46_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAR2083664.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKNLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKAIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPHTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            13527..14216
                     /locus_tag="I0B46_001355"
     CDS             13527..14216
                     /locus_tag="I0B46_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083665.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEIYEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKEIE
                     KRCTEYDLDYK"
     gene            complement(14624..15310)
                     /locus_tag="I0B46_001356"
     CDS             complement(14624..15310)
                     /locus_tag="I0B46_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAR2083666.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(15446..15640)
                     /locus_tag="I0B46_001357"
     CDS             complement(15446..15640)
                     /locus_tag="I0B46_001357"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083667.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(15690..16130)
                     /locus_tag="I0B46_001358"
     CDS             complement(15690..16130)
                     /locus_tag="I0B46_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAR2083668.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELEQLFQAYASLDDVNS"
     gene            complement(16134..16979)
                     /locus_tag="I0B46_001359"
     CDS             complement(16134..16979)
                     /locus_tag="I0B46_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAR2083669.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLEKQSKEQWLVIDGSALSLLVANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            17128..18546
                     /gene="pepV"
                     /locus_tag="I0B46_001360"
     CDS             17128..18546
                     /gene="pepV"
                     /locus_tag="I0B46_001360"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAR2083670.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(18622..19824)
                     /locus_tag="I0B46_001361"
     CDS             complement(18622..19824)
                     /locus_tag="I0B46_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083671.1"
                     /translation="MKLFKKIFFAFVLLIASYCVLLTITFFIPQHSIESNAEKSLQMV
                     EKEGMYPSINQGNMLGTRLDNFTDHLMIRKTKADPELNSLENAMSMADYPRYWHGYQL
                     FLRPLLLGMSLGSIRMIYAAILFLLIGLTSYFLTKCSDIYLTTALLISLAIGNAATFF
                     FSMQFSNIWILTLLSVLLFLTKPQLSNTNQQLFLYFFVIGSLANFFDLLTTPVISWGI
                     PVIIIYYVTNKYLMDKRSSLSKQVGSFVFTGIFWGLGYGLTWVAKWILSSIILNKNIV
                     KDAINQILFRTEGNDKYPLHRLEMLKSNIRLMYPKVAILLLLITCLVFLYFAYRKRNS
                     WRPFQLLVLFVLAAVTPYIWYNILANHSQIHYWFTYRTQIITSFAILSAFAFLIPPEK
                     SKDELNYF"
     gene            complement(19811..21598)
                     /locus_tag="I0B46_001362"
     CDS             complement(19811..21598)
                     /locus_tag="I0B46_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083672.1"
                     /translation="MHKKKENLFPTFFATLFASGFAFFTSYGLGTLNPTVIIGLAMVL
                     LLAFKQYGIELFSIKNKVAAIARLFFSAAFALTLSIGRQIQFQYVSANYTKTTIADGS
                     IFYFLVYFSIFVYSFLFLQSIYLSMPKLETFFTAQNTQAFSSRKYFLFFTVFLVLCWL
                     PYLLALYPGVVLPDSLSSVAQSMGDVPISNHHPVLFTLLVKLFVHDLPTDTINHGVFL
                     FSIFQTLITASAISYSLCWLLKRNIHFFPVLISLCYFAFAPVFPIYALNMQKDTLFSI
                     ACFLFSLAVFDFIFQREKPTILNQIYVIILALLTTYLRNNGIYVVTGTGLICGLIILK
                     QKRYQAFFQVVLGYTVIAGLLIHPLMARYAIPTETAEQLGVPLQQISRTIVMDGDLSK
                     KDKAFMNQLLPIKDYEAYSPSLADSIKWHKNFNNEFLNTHKKEFLSLWARNLPSNIKI
                     YTDAYILETFGFWVPGTKNSYGFLDTRVNTNNYGMKQVDLIEHLTGLSGMKKIIDARD
                     FFGSGTLLWLAIGSALLAIFKKKYRYLLLFLPGMLVFATIMIATPVAFSLRYVFVLAL
                     GLPVYILSPFLLDKREKMKTDIGDTHETI"
     gene            complement(21611..22564)
                     /locus_tag="I0B46_001363"
     CDS             complement(21611..22564)
                     /locus_tag="I0B46_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAR2083673.1"
                     /translation="MESREKIAVLIPCYNEEATISTVIEDFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAAHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVKNTITYLYKTKIRDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMCKDYKPLLFFGIWTIFFFLFGLVMGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYQNKEMK"
     gene            complement(22749..23099)
                     /locus_tag="I0B46_001364"
     CDS             complement(22749..23099)
                     /locus_tag="I0B46_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAR2083674.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKTSFLKEGAIEGAWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLDISRKKGAID"
     gene            23299..24378
                     /gene="pepA"
                     /locus_tag="I0B46_001365"
     CDS             23299..24378
                     /gene="pepA"
                     /locus_tag="I0B46_001365"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAR2083675.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            24521..24841
                     /locus_tag="I0B46_001366"
     CDS             24521..24841
                     /locus_tag="I0B46_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAR2083676.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VIKG"
     gene            24863..25327
                     /locus_tag="I0B46_001367"
     CDS             24863..25327
                     /locus_tag="I0B46_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAR2083677.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKALEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKQMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYAIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            25523..26128
                     /locus_tag="I0B46_001368"
     CDS             25523..26128
                     /locus_tag="I0B46_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAR2083678.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVASYGMICSARELHLPNAPEKKGILVLPEDAVVGEVFPK"
     gene            complement(26167..27456)
                     /locus_tag="I0B46_001369"
     CDS             complement(26167..27456)
                     /locus_tag="I0B46_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAR2083679.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVMMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            27884..28363
                     /gene="rlmH"
                     /locus_tag="I0B46_001370"
     CDS             27884..28363
                     /gene="rlmH"
                     /locus_tag="I0B46_001370"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAR2083680.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            28716..29030
                     /locus_tag="I0B46_001371"
     CDS             28716..29030
                     /locus_tag="I0B46_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAR2083681.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            29046..29432
                     /locus_tag="I0B46_001372"
     CDS             29046..29432
                     /locus_tag="I0B46_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAR2083682.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            29461..>30587
                     /locus_tag="I0B46_001373"
     CDS             29461..>30587
                     /locus_tag="I0B46_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAR2083683.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRD"
BASE COUNT         9687 a         5721 c         6239 g         8940 t
ORIGIN      
        1 tttgtgggcg ctaggaatca tgctagtagc attcatcgtt gctaaaaaga ataaataccc
       61 tgttgtcgaa aaaggacaga tggctcatgc tggaaaaata ataaaagagg cgattcctag
      121 ccttctattg attgtcatca ttatcggtgg tatcttgacg gggatattta cagcagtaga
      181 agcttcagcg atagctgtca tttataaatg ggttatacga ttttagaacc aggaagtgct
      241 tggaatacga tgccttgtca tacacatgaa cgtcgtatgg aagcttatgt ctattttgat
      301 tatgccaatg aagatacacg tgtgttccat atgatgggca aaccagatga aacaaaacat
      361 ttagtcgtag acaatgaaca agccattatt tcgccaagct ggtcgatcca ttcaggtgta
      421 ggtacaagca attactcatt catctgggca atgtgcggag aaaatatcac ctatacagac
      481 atggatatgg tgcaaatgga tcaattaaaa taagcataag ggagagaaaa tgatggaatt
      541 tagtatggat atgtttcgtt tagatggaaa agtagcatta gtcaccggtg ctgtctatgg
      601 tattggattt gagattgcac gctctttagc tacagcagga gcaaaaatcg tatttaataa
      661 cttgaatcaa gaatcagttg atgaaggtat agcgcattat aaagaagcag gcatcgatgc
      721 aaaaggttat gtctgcgatg tgaccgatga agaagcggtt caagcgatgg ttcaacagat
      781 caaagaagat gtaggttcag ttgatattct agtcaacaat gcagggatca tcaaacggac
      841 accgatgatc gaaatggatg cggcagactt ccgccaagta atcgacgtgg atttaaatgc
      901 cccattcatc gtttcaaaag cagttattcg ggatatgatc gaaaaaggcg gcggtaaaat
      961 catcaatatc tgctccatga tgagtgaatt aggacgcgaa acggtcagtg cttatgctgc
     1021 ggcaaaaggc ggattaaaaa tgctgacgaa aaacatcgct tctgaatacg gtcaatacaa
     1081 tatccaatgt aatggaattg gaccaggtta tattgctaca ccgcaaactg ctccattacg
     1141 cgaaacacaa gaaaacggtg aacgtcatcc attcgatcaa tttatcgttg gacgtacgcc
     1201 agcagcacgc tggggcgagc caatcgattt ggctggacca tctgtcttct tggcttctag
     1261 cgcatctgat ttcgttaacg gccatgtctt gtatgtggac ggaggcatac ttgcctatat
     1321 cggaaaacaa ccttaaaaga gctttagtac accgtttagt ggagaggttg tgaaatcagc
     1381 ggcttgaacg aataccgaag gacagctgat taaacatcat ttattcgaaa aaagaggctg
     1441 ggacagaagc gtttaactcc aagaaataag aaggaattca cgaaaattgc ttttcaaatt
     1501 tttgtgaatt tcagcttatt tccgaaggag ttgcttttgc ttccgccgtt tatacgtgtt
     1561 tagagcgtgg gataaaagtg ttctttactt ttgtcccacg ctcttttctg tgcgtcttcc
     1621 atacttcact aagtcgatcc ctcttgcttc gcaccaatcc ttcactggtc cggaaaagat
     1681 cagtgattgt tggtgtaggg ccgcggtatt cgttgcgccg accatagtat aaagcagacg
     1741 aagctcttct tgccattgct tcatcagtat aatagtttct tctacgccgt gattcatcag
     1801 atgcgtcagt acggttcctg aagtacccac tgcatttgcg ccgagacaaa gggctttaaa
     1861 tatgtcatag gcattgcgga tacctccgga ggctaggatt tccatagaag tgtctgcttc
     1921 atttgcttca agcaatgaag agactgtcga ttgcccccag tcagctaaat aactcagttc
     1981 ccgcttggag cgacgggcgt tttcgatctg ggtgaagctt gttccgcttc gaccgctgat
     2041 atcaactgta tgaactccta aagcagctag atcattgagc gtttctcttg tcatgccaaa
     2101 accgacttct tttacgatga gggggacgtc aatggctgtt tgagtctctt gaataagtac
     2161 tttccaattg gtaaaatcac ggtctccctc aggcataacc aactcttgag gtgcattcaa
     2221 atgaatctgt aaggcatctg cgtgaaacaa tcggatagct tcttgggctc tttcgacaga
     2281 agtccctgca ccgatgttgg caatgatttt cccgtgagga tattcttgtc gcataatcgt
     2341 ataagtatcg gctaaagaag ggtctttcaa ggcagcgctg actgaaccag tggcaatcat
     2401 caaatctgct tctcttgcga cgattgcaag atcttggttg attttttttg ttttttcgct
     2461 tccgccagtc atcgcattga tataaaatgg gctgcttagt gtaaacccaa ctgcctgtgt
     2521 acttagatcg acatcagaaa ctgctatttg cggcaaaggg ttatggatga tccgtacgaa
     2581 gtcaaattcg tttttttgtt tatcatgaaa ggctttagct agtgaaacat gttcgtcttt
     2641 tcgattcatc ggttttcctt tctttgatcg ctatagacat gtaacggcag tggagtgatt
     2701 tctgcttttt cccatgcact cattaaagga aggatgccag atttctggtc aacaattacg
     2761 attccgcaat cgccaccacc tgcaccagaa gattttgcgg ctccttcata ctgttcagct
     2821 aaattacaca atttgttcaa agcaggcgtt tcgatgacga ccccagtgat tgcagataaa
     2881 tcatgcagta attgtcggtt ttttcgaatc atctgttgga tcaacgttac attattttct
     2941 ttaaaccctt tgatcatttc gttgacacat tctgtactgt tttttaagaa aagctgataa
     3001 gccaccattt tgtcttctct cgaacggtga acttgatcga ccaaatcaga agtagaggca
     3061 gggctacccg tccaaccaat caataaacgt aaatcttcag gagcaatcaa tggctcaatg
     3121 gatagacctg gccaatccag ggcaagtaac tcactgatag aatgctgagt ttcttgttct
     3181 tggagccaag gatggtcgaa ggttgagaaa gcgatccagc caccataaca gctagcagcg
     3241 atgtcgccgc aggaaccatt atcttgaact gctaaattgg ctagtgctgc aatcttgaaa
     3301 atctccaatt gagataaatt caacgcgtaa aaaacattca aggctttgac cgttgcaacg
     3361 gtcacggcac cgctagaacc caatccatat ttccggccat ttgaactgtc taactcactt
     3421 gttactttca gatcataaaa ggataaaaga atgttttttt cttgtgcata cttttcagtc
     3481 aaacgaatag cagcaaggat ataatgaaaa ggattttccc gaatatctaa aactaattct
     3541 ccgttgcgtc ttgtccaacg tacaggcatc ccactatatt gtgcagattg gatacttccg
     3601 acttttcgtg cggattctac agtgactgtt acgaattgat cgactgcagc gataactgct
     3661 ggatgtcctg tttcaacaac tgcatattct ccggcaatat agagtttgcc tggtgcagat
     3721 acttcaatca taaaaatctc cctttattca ataatcgcaa ttcctggacc agcatgggca
     3781 gtaattactt gttctttgct aaataatttg gtaaatgttt cttgtacctc agataaatgg
     3841 ctgttttcca ctaaaacttt gacattaggg ccagcatcca tcgtaaagta gcatggaatg
     3901 cctttttttc tcaattgccg caccgcatcc atcgctttta agctgtcagg agaccagtaa
     3961 gtgaatggag gctgggcagc taaagtcgtt ccatgcattt ttagtccgtt tctttccatc
     4021 gtttctccga gaagttggaa atcttttgtt ttgattgctt gtttcaattg atatagatcc
     4081 ccttcaacgg aatctaacca gccttgataa aaactggatg tttcgactgt tcgacgcatc
     4141 ccatttctgc tggacacttc ttttttctgg tcgttgatca aaacgaaaac cattgctaaa
     4201 tcgtcttcga aagaatcgga agggactggc ttagcgtaag aacttaagtc gtcatggcct
     4261 ttttcccatt caacgaaacc accgaaaata cttcggcaag ctgaaccaga cccgcgtcga
     4321 gcaaatcttg aaagagagag atcgtctaat ccaagcttaa gagcagtatt gcaagctcct
     4381 gctaaagcag ctagcccgct ggcagaggag gctaatccag ctgcggtagg aacgaaattc
     4441 tgactgatta ctgaagcttt tagtgagagg ccggcttctt tacgaaaaag gtcaagaaac
     4501 tgactgactt tttttgttgc tttttcgtct tgtaaggtgc catctagata aaattcatct
     4561 accatatagc tatcagaaaa gatgacttct gtctctgtat aaaatgcatc caatgttaac
     4621 gaaaggctgt tgttcattgg aaggatgagt tcttcatttt tcttacccca atatttgatt
     4681 agagcaatgt tggtatatgc gcgtgctttg cctttaaaca taggtatgta ctcctaatcc
     4741 ttgtatccaa gtttcagcag cacccgcatc ttcaagtgct tgactgattt cttgggcttt
     4801 tgtttttgtt tgtgctaatg caatcataca gccaccgcgt ccaccgccag ttaatttggc
     4861 acccaatgcc ccggtatctt ttgctgtttg gatcaataga ttaagaaaat cattgctgat
     4921 tgtcagcttt tccagaagtg actgtgattc atccattgtc tgtgccaacg tttctggtga
     4981 attctcaatg attgcttgtt ttgcttgctt cgtcaagtat cctaattttt ggatcatttg
     5041 tccagtttca tgaggctggg tttcaaagag gtgagcgaca tctttgacgg cttctcttgt
     5101 ttgtcctttg attcctgtat cagcgacaat caaaaaagca tcgatgttca aagaaaagta
     5161 gtcaaaagga tgcccttttg taaaataaat cggctgcaag ctgctagtag cggctgcatc
     5221 gattccacta ggattaccgt gggcgatttt ttccgaaagc tggacatttt ctaatagtat
     5281 ttcacgagac aaaggaaatg ctaagtaatc ataaaaagca cgagtgactg ctgtggcgac
     5341 tgcagcgctt gatcccattc cacgttcagc aggaatcgtg ctttcaatgg tcagttgcaa
     5401 gtttttttgg atgtcatgct gtttttttaa gtgaatgaat aattttttga tattttttaa
     5461 tgcatgagga gcttcggcta aatttcctga atagtaggaa gaaaccagtt gatcgtccat
     5521 tgcatccagc tcttcaagga atgcggtgac ttttgttgca tagaaaggaa aggcaatcgc
     5581 tggttctcca taaacaaccg cgtgctcgcc catcaatatg atctttccgc ttgattctcc
     5641 ttggccatag tttgccataa tctaactcct tctcacgtaa aagtgatttt taagcaaaaa
     5701 ataaagcgcc caaaacgagt tgcgctgctt cacgaatttt tttgtccact aagggtctca
     5761 aaaaacattc ctcctttatt ttactaaaac catctcagaa gtaaaactaa gaatacgttt
     5821 tctaaagaaa aaacactttt ttcttaagaa ttttttgact ttttttcttc tttgactaga
     5881 aaaaatacaa ttgacactac tagatattgg gggtatattt aaaaaaggtt ggagttaaac
     5941 acaatattta gtgatgaagt gagctggagg taaatgagcg tgatcgtttt agcaggtaca
     6001 attggtgccg gaaaatcaag tttaacagaa atgatggctg aacactttga tagtcaggca
     6061 ttttatgaat cgattgatga taatgaagtc ttgcccttat tttatgcaaa ccctgaacag
     6121 tatgcttttt tgctgcaaat ttattttttg aataaacgtt ttgcaagcat caaacaagca
     6181 atgaaagatg acaataatgt attggaccgt tcgatttatg aagattcact gcttttccat
     6241 ctaaatgcag atctaggaag agcaacagaa acagaagttc gtgtgtatga tgaactgctg
     6301 gaaaacatga tggaagaact tccctatgct gcccataaaa aacatccgga tttattagtt
     6361 catattcgtg tatcttttga tacgatgtta gagcgtatcg aaaaacgtgg tcgttcgtat
     6421 gaacaattgt cctttgaccc aagtctatac gattattaca aagaattgaa cagacgttat
     6481 gatcaatggt atgaagaata caaagaaagt ccaaaaatcc aaatcgatgg agaccgctat
     6541 aattttgtgg aagatcctca agcaaaagaa gaagtcttga aaatgatcga agaaaaatta
     6601 gcagagattc gccaaaccaa agtatccatt tgatttacag ttgctcaaat aaagggtaaa
     6661 cctttatttg agcgactttt ttctttatac tggaaacaaa tgtcaggagg atagagtatg
     6721 cgggaaaaac actattttaa tcaagaccgt aatttttgga tcaatgtaca gcaaaacgat
     6781 gtgctagctg ttaaaaactt aaaagaagaa tatggcatta gtgacgagat gctgacttat
     6841 tctctagata aaaatgaacg ggcgcgcgta gagtacgatt cttttgatga agcgttactg
     6901 cttgtatcaa atgttccgca ccagcaaaaa gtcgataatc attatgaaac tagtcctatc
     6961 gcttttattt taaaagaaga cggtttgttt acatttacca cgccgaatac cgagtacgtg
     7021 attcgactaa ttcgatcgct tttggaacgg atgccagaca tgagtgtata cagtttattg
     7081 tttcgtacat tgtttttgat ttctgattcc ttttttcctt taatcgagga agtgaatagt
     7141 gaacgtcagc ggcttaatct gaaattaaga gaaaaaacaa cgaacaagaa tctgctgcaa
     7201 ctttcagatt tagagatcgg tttggtttac ttagttacag gaacaaaaca aaatgtagta
     7261 cttttagaac aaatcaaggc actggctatt tatcgcaagt tgtcagaaaa ggaaaaagaa
     7321 gagttggacg atgcactgat cgaagcacga caagcagttg aaatgacgaa cttagcttcg
     7381 cagattttag accagctctc tggcacttat aataatttgc tgaataacaa cttgaatgac
     7441 acgatgaaat ttttgacggt ctggtcgctg atcttgaccg ttccaactat tgtgacagga
     7501 ttcttcggaa tgaatctcca gttacccttt acccattccg ttttcggatg ggaaatcgcc
     7561 ttgatcatca gtttggtctt atctatttgg atgcttattg cactgtggcg gcggattcgt
     7621 tgatctagtc tttgatctgc aaaccaaggg cttgggcgat cacaagtgct tgctcttgat
     7681 tgacgattag tcccgccaaa gaatcttgag ataaagcgat tgttgtgaag aaattagtcg
     7741 taaagtctaa tcccttcaga gaagttcgaa accagttcga accggttaac tgattgtcag
     7801 acaaaaaaag gtttttccaa gtgacctcat aaaattcgct gtcttctaat tgacaatgat
     7861 cgaaggagac agctttcaag tttgtactac taaaggaagc catcgaagca atacaatcag
     7921 tgaaagtaca gtctcgaaga taactttcgg caaaattcgt acctgttaat ttacattgat
     7981 gaaacacgac ttggtggaaa ctgccaccaa tccactccag attggaaaaa tcacattttt
     8041 caaaaatgac attactgcat tcaaaatgac gtaaacctgt tttttgcatc gtaagttttt
     8101 ggatatgact ctctcttaat accatatttt tggcatcgag atagctttgg ttacctccgt
     8161 tgatcagaag accagagaag atctcctcat cttctaaata aaactctccc tttgtaagaa
     8221 gaggaagaga aggtggaata ggttcgattt ttttcatgtg ggttcacctc gtttttcttt
     8281 agtatagcaa agaagcggga atcactgcaa gaaagcctgc taatctaatc attttcagaa
     8341 aaggaagaca aactgtatga aaaacattga caaaaagatt agtttttgct atactataat
     8401 ggttgagaaa taaaagcaga agcacccgct tctcgcctta gttgataatg tcactgggct
     8461 agatagtttt cactggttct attaggtgaa gtacgcgcgg gttctgtaag agaactcgtt
     8521 tttttattgc cgtttattcg gtaagctttt ctttctctca taatatttgg aggtgaatga
     8581 ccatagcaaa ggatatgatg gttaacgacg gcattcgtgc acgagagtta cgattgatcg
     8641 gttcagatgg tgagcaatta ggagttaaga caaaagcaga agcattgaat attgcagaac
     8701 aagcaaactt ggatcttgtg ttagttgccc ctggtgcgaa accaccagtt gcgcgaatca
     8761 tggactacgg taaataccgt ttcgaacaac aaaagaaaga ccgcgaagcg cgtaaaaaac
     8821 aaaaagtgat caatgtaaaa gaagttcgct taagtcctac gatcgacttg aatgacttca
     8881 atacaaaact tcgtaatgca cgtaagttct tggaaaaggg cgataaagtg aaagcttcta
     8941 tccgtttcaa aggccgtgcc attacccata aagaaattgg tcaaaaagtc ttgaatcgct
     9001 tagctgaaga aactgcggat attgcaacag tggaacaaaa agcgaaaatg gacggacgca
     9061 gcatgttctt gacgctggca ccgaaaaacg acagtaagta atttgaatga ctaactggct
     9121 gttggacagc cgatagtcgg ataacgaaag attttgagga ggaaatatag tcatgccaaa
     9181 acaaaaaaca caccgcggat cagcaaaacg tttcaaacgt actggtaaag gcggattgaa
     9241 acgcttccgc gcgtttacaa gtcaccgttt ccacggcaaa acaaaaaaac aacgccgtca
     9301 attgcgtaaa gcaagcatgg tttctagtgg cgacttcaaa cgtattcgcc aacaactagc
     9361 aaaaatgaaa taagaagtac aaataacaga atctgaagat ttatttttat taggaggaat
     9421 tatacatggc acgtgttaaa ggtggaacag taactcgtaa acgtcgtaaa agagtgctaa
     9481 aattagcaaa aggttactac ggttcaaaac acactttatt taaaacagca aaagaacaag
     9541 taatgaattc atacaactac gcatatcgcg atcgtcgtca aaagaaacgc gacttccgta
     9601 aattgtggat tgctcgtatc aacgcagcag ctcgtatgaa tggcttgagc tactcaaaat
     9661 taatgcacgg tttgaaagtt gctgaaatcg acatcaaccg taaaatgtta gctgacttgg
     9721 ctgttaacga tgcagcagca tttactgcat tggctgacca agcaaaagaa gctttggcta
     9781 aataagctaa acaattataa agaggaaaga agataacgaa aagttatgtt ctttcctctt
     9841 tttttattta caatcctcca tcaaatgaaa caggacaata acgtataagt ctaaaataac
     9901 ataattaagg ttttttaatt gaaaactgaa catttatggt catcctttaa tgaaagcgct
     9961 aactacataa gttagaatga gtttgactga tatattagtt tgcttatcag aaaaacaaaa
    10021 tggacggagg aataacatga gtgatattat tattggtatt ttgttagtta tctcattctt
    10081 tttcatggtc tggtactgtg taaaaggcta caacttgatg gtcggctttt gtcatcatgg
    10141 ctactgtatg gatgggatta gcgctagtcg gaaacacgtt ttcaccaaac tcggcgatgg
    10201 aagggcaagg agtaattgat gtgttgacgc atatctatac gacggggcca gctgagtacg
    10261 ccaaatcgat cttggtaaat gtcttctttg gtgccttttt cgggcgtgtt ctagtagata
    10321 gcggaatcgc agctaccctg attcgaaagg tagttgaact tggaggggac aagccaagaa
    10381 tcacgatgag cttactatgt attgtaaccg ctgttatatt catgtcgatg acaggaatcg
    10441 gaccagtgat ttctattgca gttattgtgt tgccgatctt gatgtcatta ggtatttcag
    10501 taccggttgc actgttctca tttatgggct cgattatggc tggaatcttt gctaatatcg
    10561 tcaatttcaa acaatatcaa acgatttatg ctggtttcaa tccagcggct gaaagctata
    10621 cttataatga ctatttccaa attggtatga ttgggatgat tgtaagctta gttgtggtac
    10681 taacggtcgc taatatatca atgaacaaaa agaaacgcta tgcaatggca gcaaacgtac
    10741 cagctgaagg tggcgacgca ccaatgatct catggttagc cgtattatta ccagttttag
    10801 gggtcgttct tttagatctt caaatcatct taggttttat tctagcaggt atctgggcat
    10861 tgctgtttac tggaaaattg cgcggaggtt ataaagaaat ctgtcgtcag tttgcgaaat
    10921 tatttacaga tggcgcagtc gatgttgcac caatggttgg tttcttgatg accttagcga
    10981 tgttcaataa ttcagctgcg tatgcttctc cttatttttc agctattttt ggagattgga
    11041 taccgcaatc accattagtc ttggcaatcg tatttgctat attgacacca ctaggatttt
    11101 tccgcggtcc aatgaatcta gtcggttcag gctcagctat tttagctgtc gtgttagctg
    11161 ttaatccaac gatgtcgcca gcctttttgt tcccgttatt tgcaattaca acgattgctc
    11221 ctcagcattt agatattaca caatcttggg ttgcttgggg attaggttat acaaaagtaa
    11281 cttctcgaga atatatgaag aaatccatcc caactggttg gatcatcggt gcgatcttat
    11341 gcctgatcac gttcttattg tacgggaacg cctagtaaac aaaacgtcga taaaaacgat
    11401 tagataaatg gaggaaaaga aaatgaaacg ttttgtgcga atgggaattg atgttggcgg
    11461 tacacatacg aaagcagtcg caatcgataa tgcgacgcac gaaatcatcg gaaaatcttc
    11521 agtgaaaaca acccacgatg atgtccgtgg agtagccgca ggtgtcgttc agtcttttca
    11581 aaattgtttg cgggaaaata atatcagtcc agaagatgtt gtgtttgttg cacattctac
    11641 cacacaagcg actaatgcac tgattgaagg agatgtagca aaagtcggcg taatcgggat
    11701 ggcaaaaggt ggattagaag gttttttagc aaaacgacaa actagattga acgatatcga
    11761 tttagggaat aagaaaaaaa tagagattgt caatgcattc ttacctgtca aaaacttaaa
    11821 tgtagaccgt gtttcagaaa cgatttcttc tttagaaaga gaaagagcgg aggtcttggt
    11881 ttcttctatg gcatttggag tagataatgg ggaaccagaa cgagttgttt atgaagcagc
    11941 atctgtgaaa gcgatcccta caacgatggc ttctgacatc acaaaattat atggattgac
    12001 aagaagaact cggacagcag caatcaacgc atctatttta ccaaaaatgc tagatacggc
    12061 aacctcgaca gaagactccg tacgagaagc aggggtcaat gtttctttga tgattatgcg
    12121 cggagacggc ggagtaatgg aaatcaatga aatgaaaaaa cgacctgttt taaccatgct
    12181 ttccggtcct gctgcatcag tcatgggctc tttgatgtac ttgagagcgt ctaatggtgt
    12241 ttattttgaa gtaggcggaa caacaaccaa tatcggggtc attaaaaatg gacgtccagc
    12301 aatcgattat tcaattgtag gtggccaccc aacatatatc tcttcattag atgttcgggt
    12361 tctaggtgtg gcaggcggtt cgatggtacg ggctaatcag tcaggaatca tcgatgtcgg
    12421 tccacgttct gcacatattg ccggtctaga ttatgcagta tttacggaga cagaaaaaat
    12481 caaaggtcct aaggttgaat ttttctctcc taaagaagga gaccctgctg attacgtcaa
    12541 ggtagtgatg gaagacggag aagaagtgac tattacaaat acatgtgcag ccaatgttct
    12601 tggattagtg caagaagaac atttttctta tggaaatgtc ccatctgcac gaaaagccat
    12661 acaggcatta gcagactatt gtcatacgac ggttgaagac atcgcagagc agatcatgga
    12721 aaaatcctat gctaagattg aaccggtgat tttagaattg gcagacaagt atcacttgga
    12781 gaaagatcag atttctcttg taggtgtagg gggcggcgct gcctcattga ttacttactt
    12841 tagtaataaa atgggtgtga aatattcgat tcctgaaaac gccgaagtga tttcttctat
    12901 tggtgtagca cttgcaatgg tccgagatgt tgtagaacgt atcatccctt cgccaagtaa
    12961 agaagatata cgatcgttga aaaatgaagc gatgaataaa gcgattgaat caggcgctac
    13021 accagaatcg atcgaagtcc acgtagaaat cgatccacat acttcaaaag taacggctat
    13081 cgctactggt tctacggaag tgaaagcaac cgatctaacc aaggagatca caacagaaga
    13141 agcactagag cttgctgcag aggatatgcg attaaataaa aatgaagttt gcttattgga
    13201 aaatacacct ttcttttatg tatgcggcga acagaatcgt tcaaaaaatg ctggaagtct
    13261 tcggatcatc gatcaaaaag gatttatcaa ggtccaacgt ggacatgctt catgtatgaa
    13321 aacaactgca gccaattata tgacagctgt cgaacaactt tgggaagata tggcagttta
    13381 tcaaactgaa ttgattgccc gaccagaatt ttatctctgc ttaggagcaa gagtctctga
    13441 ttttacagcg acggatctgg aacaactaca gcttttgatg gatctggaag tatctactat
    13501 ggaaccggag gaagaagtga tcgtcgttgc cggaaatatc aaacaaacat aagttagaag
    13561 agttactaaa cgaattacaa cagatccccg aagaaatttg gggattctac cagtttcggc
    13621 gagatttatt ttggaaaaag attcctttgt ccaagcagaa aattcttata caacaatcaa
    13681 tcgattgtgg tatcgaaacg gcatgtagca tcaaaaaaaa gtaccctttt gccgatgtag
    13741 gagaaatcta tgaacaaatg gctgttccaa tcgtttcctg tgaatcagag caaataaacg
    13801 aacgaatcac ttttgcaact tatgcagaag atgaagggat ccgattgatg acggagccgt
    13861 tagaaaaatt aaaatgttca ggactagctt cgatctctaa agaaacagcg caagctttga
    13921 ttattggaca tgaactattt caccacatcg aagcttctgt taagggaatt tatacgcaaa
    13981 gcgaaaaaat tgtgctgtgg cggttgcctt tttatacaca tcaatcaaac attcgtgcat
    14041 tgagcgagat agcagcgatg agtttttcaa aagagatgaa tcaaagcgat ttttcgcctt
    14101 atgtattaga ggcagtctta ctttggccat ataatgaaat tcgtagccaa gggatcttag
    14161 aagaaataaa agaaatagaa aaaagatgta cggagtatga tttagattac aaataaagag
    14221 actgtgacaa aagtcgtgtc acagtccttt ttttccgaat aaacggtgtt ctcctgtcaa
    14281 ctcctcggtc acaagtcaaa atattcaaca aacatgagaa gctgtttttt gaaattgttc
    14341 cttgtgactc ggagatgaac ggcaggctca cgacctcttg gaaaaaggtg ttcaaaagct
    14401 tgcatctgcg gtaataagaa cagccaagtt aaaaacataa gaagctattt ttacttggct
    14461 gttcttatta cttgatgcag ggcagctttt tcacaacctc tcaataaacg gtgtgcaatc
    14521 agctgaccct cggtattagc tcaaaaaaaa cggcaaaata tgaggaacta ttttgccgtt
    14581 tatttctcta atactcaggt caaaacgctg attccaaaac ctcttatttg atgggtctgt
    14641 cctgaatatc ttcaaaataa tcggggtaat tatgaatcac gtattcttgc tcctcgataa
    14701 tcatatgtaa atgcatcgtg caaagaaatc cgacttcatc aaagttatga tcaatcattg
    14761 attgaagcat tgtctgatgt tcatctaata ctcgacccca gtctaattct gaaatcgtca
    14821 aacgtaacca tctgaaacgg ttcaagtgcg tactataact ttctagccaa tcccaaattt
    14881 ctcgttttcc agcaatttca taacaagttc ggtgaaaatc agtatccaaa tcaaaaaaag
    14941 cttttttatt ttgagcgatc actgcctgat cggtttgttc tagtattttt tctagtttag
    15001 cttgatcttg cttggtcatt ttcgcactgc attcttgcag gatcggtttt tccaactttt
    15061 cccgcgtata acgggctaat gaagcagatc ggatatcgat ttttgaaaca taagtgccgg
    15121 actgtggaat ggtttttaca agctcttgat tgcgcagttg gatcagtgct tctcgaatcg
    15181 gtgtacgccc gatagctagt tgttcttcta acactttatc ggagatttta cttccaggtg
    15241 ctaactcgga ataaatgatt tgtttacgta tagattcata agcttgctgt tgcaaattat
    15301 tgataaccat ttaaccttcc tttctatgca gatataatct attataacat aaatcgctta
    15361 caaatcgtgt gggcataaaa acacagtctg caaaaaaagc cctgataggc atgcctatta
    15421 gagcctcgaa aagaaaaata ttgaatcaat ctggtcgtac ctcttggatc ggtttgacac
    15481 gaccgccgat tcttttagca aaagcttcgg attctttttt gtctttgaaa atgagcagat
    15541 tgtctaagtt tttttcccga gtgcgacagc cagttttatc gatgtaaccg tgcattactt
    15601 taacgacata catggggatc acctgctttt ttcttttcat tatacgaact actcgacaca
    15661 atacaagaaa aatgcttaga cggtatttat taagaattta catcatccaa agaagcgtac
    15721 gcttggaata actgttctaa ctccggattg actttgctca gcttcactaa tcgttgcgaa
    15781 tcagaaagta aaaaacagtg tttactactg ccagtagttt ttaatttttt gtcttcagtt
    15841 cccacaattt cgtagctaaa atccaaccgt gttcccgtat acttttggat agttgggtgg
    15901 atgctcactt tttctccgta atggatcatt tctttatagc tgcaatttac ttccagaacg
    15961 gggatgatga tcccagattc ttcgattttt ttatagggaa accctagatg taccaataag
    16021 gctacccgag cctcttcaaa ccagcggata taattgctgt gatgaataat ccccatttga
    16081 tctgtttcat aataaaaagg ttctcgcaca taacctgtaa atttctccat gactcatgcc
    16141 tcgttttcca ttgatttttt acaaaaataa ttcatccact tcggaagtgc ttcgctgatt
    16201 tgtgtcggca aaacaatata ttgttctttt gccagctggt ctccaatgaa actatgaaga
    16261 taaacagctg ctgcaatcgt ttggtcattt tgaggaaact gggctaaaaa tcctgtgatc
    16321 atcccagcta aagtgtcacc catgccgcct gttgccatag cagcagaacc taaaggattg
    16381 gtgtagaagt gctggtgacc ataaattttt gtctcatgac ttttcaaaac gagcacagca
    16441 ccgatttcct gttgcttctt tttgttttct ttatcccgtt gttcttgtat cggtagagaa
    16501 ctcaaccgtt gccactccat ttgatgaggt gtaagtacag tttgttcggg atagggtagc
    16561 aaaagatgat tagcaacaag taaacttaaa gcagagccat caatcaccag ccattgttct
    16621 tttgattgtt tttctagtac ttgtttgaaa cgttccaaac tcttagaaga agtacccaat
    16681 ccgggaccaa tcaagagtac atctgcttgc tttattacgg aaagaaactg gtcagtatcc
    16741 gtccaatcta caatcattgc ttctggcaga tgactgtgaa gagcactccg atttttttca
    16801 tcagttacta cagtcgtgag acccgctcca ctgtgtacgg cagctaaagc actcataatg
    16861 attgctccgc catattgttc attgccgcca atcaaaacga tacgtccaaa tgttccttta
    16921 tgtgaaatct ttggacgagg tgtgatgact cttttcacta gttgttcgtt tatcagcatc
    16981 gaaaaacctc cttttactca ttatagcgta tatttttttt agagaaaaac agataagaaa
    17041 aggaattgtc agattttttg aactattttt ttctgtaatt tgtggtatca tgaacacgaa
    17101 gacttaaata agtggaggat taactttatg acaattgatt ggcaaaaaga agtagaagca
    17161 cgtaaagaag acttgcttga agacttgaaa aatcttttac gtgtcaacag cgagcgcgat
    17221 gattcaaaag taacaccaga tgcaccattt ggacctggac ctcgcgacgc attgaaacat
    17281 atgttagctt atggcgaacg tgatggattt aaagtaaaaa atgtcgataa ctatgcaggg
    17341 catattgatc ttggcgaagg agacgaaacg ctagggatat ttggacacat ggatgtcgtt
    17401 cctgcaggag atggctggga cacagatcct tatgaaccag taatcaagga tggaaagatc
    17461 tttgcacgcg gatcaagcga tgacaaaggt ccaagtatgg cagcttacta tgcgatgaag
    17521 atcattaaag aacttgactt gaaactatca aaaaaagttc gcttcgtagt cggcagcgat
    17581 gaagaaagcg gctggggcga tatggcttat tatttcgaac atgaagaaga accagatttt
    17641 ggtttctcac cagatgctga attcccaatc atcaatggtg aaaaaggaaa tgtttcttta
    17701 gctttacgct tcaaaggcga caatgcagga gattatgtct tgaaatcttt tgtttccgga
    17761 cttcgcgaaa atatggtgcc aggaacagca actgcagcac ttcaagtacc aagtgcagat
    17821 gcagctatcg cgatggaaga agcattttac caattcatcg aagcaaatcc agtaagcgga
    17881 acgattgaag cggacaatac gtatgtaaaa atcgaattag ttggtaaagg cgcacatggt
    17941 gcaagcccac aatctggtat caatgcaggt tctttcttgg cattgttctt agataactat
    18001 gaattcctag gatctgctaa acaatttatc catgtagcag ctgcttatgt ccatgaagat
    18061 ttttatggag aaaaactagg agtcgcttat gaagatgaaa aaatgggcaa attgacaatg
    18121 aatgctggac tatttgcttt tgaagaaaat ggtacagagg aagcgaactt catcaatatg
    18181 aatttccgtt ttccaaaagg tgtaacagta gacggattgc aaagtgacat cgaacaaaca
    18241 gtggggcaag aaggcgcaac agttactcga ggtgctcgcg taatggaacc acattacgta
    18301 ccaatggatg atccattggt ggctacatta ttgcaagtct atgaagacca cactggagaa
    18361 aaaggctacg aacaaatcat cggtggcgga acttacggac gtttgttgaa acgtggcgtt
    18421 gcttatggag cgatgttccc aggatatact gatacaatgc atcaagcaaa cgaatttatg
    18481 agtcttgatg acttgttcag agcaacagca atctatgcag atgccatcta tcgtttggcg
    18541 aaataaaaat actaaaaaac tccggctatc tctgacagct ggagttttta tgtttaaaaa
    18601 tgaattagta catcggtatt cttaaaagta atttagttcg tcttttgatt tctcaggagg
    18661 gatcaaaaag gcgaaagcac taagtatagc aaaacttgtg atgatttgcg tacgatacgt
    18721 aaaccaataa tgaatctgtg agtgattagc taaaatattg taccaaatat acggcgttac
    18781 agcagctaaa acaaacaaaa ctagaagttg gaacggacgc cagctatttc gtttacgata
    18841 agcaaaatag agaaaaacaa gacaagtaat caatagcaat aatattgcta cttttggata
    18901 catcaatcta atattgcttt tcagcatctc taatcgatgc aacgggtatt tgtcgtttcc
    18961 ttccgttcga aagaggattt ggttgattgc atcttttaca atgtttttgt tcaatatgat
    19021 cgatgatagg atccatttag ccacccatgt caaaccatag cctaatcccc aaaagatacc
    19081 ggtaaaaaca aaactgccta cttgtttgct caatgaactg cgtttatcca ttaaatactt
    19141 atttgttaca taataaataa tgatcacagg tatcccccaa gagatgaccg gtgttgtcaa
    19201 caagtcaaaa aagttagcta aagaaccaat gacaaagaaa tacaagaata attgttggtt
    19261 ggtgttggag agttgtggtt ttgttaaaaa tagtaataca gacaataaag tcaaaatcca
    19321 aatatttgaa aattgcatac tgaagaaaaa ggttgcagca tttcctatag caagcgaaat
    19381 caacaaggct gtcgttaaat agatgtcgct acacttggtt aaaaagtagc tcgtcaatcc
    19441 aatcagcaga aaaagaatgg ctgcgtaaat cattcgaata ctccccaaac tcatccctag
    19501 cagtaaaggt cgtaaaaaaa gctgataacc atgccaataa cggggataat ccgccataga
    19561 catcgcgttc tccaaggagt tcaattcagg atctgctttt gtttttcgta tcatcaaatg
    19621 atcagtaaaa ttatctaacc gagtgcctag catatttcct tgattgatag aaggatacat
    19681 tccttctttt tcaaccatct gtagagattt ttctgcattc gattcaatag agtgctgcgg
    19741 aatgaaaaaa gtaattgtca gtagtacgca atagctagcg atcaagagaa caaaagcaaa
    19801 aaatatcttt ttaaatagtt tcatgagtat ctcctatatc tgttttcatt ttttctcttt
    19861 tatctagtaa aaaaggagag aggatataaa caggtagacc taatgctaaa acaaacacat
    19921 accgtaaact aaaagccaca ggagtagcaa tcataatagt agcaaaaaca agcataccgg
    19981 gaagaaaaag gagtaaatac cgatattttt tcttaaagat tgctaaaagt gcactgccga
    20041 tagctaacca cagtaatgtg ccggaaccaa agaaatcacg agcatcgatg atttttttca
    20101 ttcctgacaa tccagtaaga tgttcaatca aatctacttg cttcatacca taattattcg
    20161 tgtttacacg agtatctaaa aagccgtaac tattttttgt accaggtacc cagaacccga
    20221 acgtttctaa aatataagca tctgtataga tttttatatt acttggtaaa tttcttgccc
    20281 atagtgatag aaattctttt ttatgtgtat tcaaaaactc attgttaaaa tttttatgcc
    20341 acttgattga gtctgcaaga ctaggagaat acgcttcata atcttttatc gggagtagct
    20401 gattcatgaa tgctttatct ttcttagaca agtcaccatc cataacaata gttctagaaa
    20461 tttgctgcaa aggaacacct aattgttcag cagtttctgt aggaatagca taacgagcca
    20521 tcaacgggtg gatgagtagt ccagcaatca ctgtataacc cagcaccact tgaaagaatg
    20581 cttgatacct tttttgtttc aaaataatca agccgcaaat caaacctgtg ccggttacta
    20641 cataaatccc attatttctt agataggtgg tcagtaaagc aaggatgatt acgtaaatct
    20701 gattgagaat cgttggcttt tctcgttgaa aaataaagtc aaaaaccgct aaggaaaata
    20761 aaaagcaagc gatagagaac aaagtatctt tttgcatgtt caaggcgtag attggaaaaa
    20821 ccggtgcaaa tgcaaaatag cataaactaa tgagtaccgg aaaaaaatgg atgtttcttt
    20881 ttaacagcca gcaaagagaa taacttatcg ctgaagctgt tatcaatgtt tgaaaaatcg
    20941 aaaataaaaa tacaccgtga ttgatcgtat cagttggtaa atcatgaaca aatagcttga
    21001 ccagtaaggt gaataagact ggatgatgat tgcttatagg aacatcaccc atagattggg
    21061 cgaccgaact taacgaatca ggcaaaacca ctccaggata caatgccaac agataaggaa
    21121 gccaacacag aactaaaaag acagtaaaga ataaaaaata ttttcttgaa gagaaagcct
    21181 gtgtgttttg agcagtaaaa aatgtttcca gttttggcat tgataaataa atcgattgta
    21241 aaaataaaaa agaatacaca aaaatggaga aataaacaag aaagtaaaaa atacttccat
    21301 cagcaatcgt tgtctttgta tagtttgctg agacatactg aaattgtatc tgtctgccaa
    21361 tagaaagtgt aagagcaaaa gctgcagaaa aaaacaatcg ggcaatagct gcaactttgt
    21421 tttttattga aaacaactct attccatatt gcttaaaagc aagtagcaaa accatggcta
    21481 aaccaatgat tactgttgga ttcaaagttc ctagaccata agaagtaaaa aaagcaaaac
    21541 ctgacgcaaa taaagttgca aaaaaagtag gaaatagatt ctcctttttc ttatgcataa
    21601 aaaaggcctc ctacttcatt tctttatttt gatattcgtg gtagacatgg tataaattca
    21661 attcatattc tttcttggca tttgtgacga ccgtatccaa aatcaaccct gttactaatg
    21721 aaagaagggc taaagtcatc aatccagtac ttaaaatcgc agaagggatg cgtgtaataa
    21781 agcttgtcag catatattca cgaatcacag gaatccccat gaccaatcca aataaaaaga
    21841 agaagatggt ccagatccca aagaaaagca aaggtttata atctttgcac attttgatga
    21901 tcatcatgat cactttgaat ccgtcagaga atgtattcaa ttttgattcg ctaccttcag
    21961 gacgatcgcg ataatcgatt ggaatctcaa cgaacttgaa ttttttatct aaagcatgaa
    22021 tcgtcaattc tgtttcgatt tgaaaaccag aactcataat agggaagctt ttgacaaaaa
    22081 ttcgattgaa tcctcgatag ccggtcatga catcgcgaat ttttgtttta tataagtaag
    22141 taattgtatt tttaaccaga ttattcccaa aatcatggaa aggacgctta ttttcatcaa
    22201 aataggttcc atttgataga cgatcgccaa tgaccatatc tgcttcgcca gaacgtaatt
    22261 tctcaagcaa accatgtgca gcttctgcag gataagtatc atcaccatct accatcaaat
    22321 aataatcggc atcaatatca aaaaacattt gacggattac attccctttt ccttgacgtg
    22381 gttctttctt tacgattgct ccgccttgaa cggccaattc gtatgttcga tcggtcgaat
    22441 tattatcata gacataaata tctgcttctg gtaattccct tttaaaatct tcaataacag
    22501 ttgaaattgt cgcctcttcg ttataacaag gaatgagcac ggcaattttt tcacgtgatt
    22561 ccatttgtca ctctccttat ataataaaac taatcttatt tcaataatac cgtataataa
    22621 ctacaaatac tagctattcc aagaggaaat agccctattt ttataaaaat cacccactat
    22681 aaaagaagga taggatacct ctcttttaga tagtgggtgc gtattcgtta tttcattttt
    22741 ttgacgtatt aatctattgc gccctttttt cttgaaatgt ctaatacggt tcctgtatga
    22801 gcatctgcta aaaattcata ataaacagtt tcgccatctt ctatccggga aagcccaccc
    22861 cgataagcac tcgaccgaac ggcgaatttc cgaataggtt ctttttcaaa agagatccaa
    22921 gcaccttcga ttgcgccttc ttttaaaaaa gatgttttga tattttcaag cacttggtct
    22981 gctgataaca cttttttgcg atgataccaa atcgttgaag ctaccccact caataatcct
    23041 agaccgatcc caacggctaa cccgccttta aaatagctta attcgctttc ttcatacatg
    23101 tggatatacc tccttcttct cacttgctac tctttgtgtt tattttacca cactctcata
    23161 ttttctttga aataaataac cgctaccaga aaaaataagc tataataaat acgatcggtc
    23221 tgcagaaaaa atcatatttt atccgcagat caatataagt ctgagaggag tttagtctga
    23281 aatttagacg aatagcatat ggaagaaaaa acatttcaac gaatcaaaga gttaacagaa
    23341 ttacaaggaa caagcggttt tgaacaagac gttcgcgcct atatggaaag tcatatggaa
    23401 ccgctggtag atgagttaca gttagatggt ttgggcggca tttttgggct gcgccaccat
    23461 gaagaagcgg atgcacctcg tgtaatggtc gcagcacata tggatgaagt cggattcatg
    23521 ctgacacaaa tccaagaaaa cgggcttttt aaagtcgtac ctctaggcgg atggaaccct
    23581 tacgttgttt ctgctcaacg ttttacactt aaaacctcta ctggtaagaa ttatccatgt
    23641 atttcttctt ccgttccccc acatttatta cgaggaacaa gcggacaaaa atcagtagaa
    23701 gtaacagata ttttgttcga tgctgggttt gaatcaagag aagaggcaat gagttacggt
    23761 gttcttccag gagacacaat cgttccttac gctgaaacga tcaaaacagc caacgggaag
    23821 aatatcatca gcaaatcgtg ggacaaccgc tatggctgta caatggtatt agaagcttta
    23881 gaagcgttac aaaatgaaac attaggtcat acattgattg caggcgcgaa tgtccaagaa
    23941 gaagtaggct tgcgtggttc taaagcttct gtcaataaat tcaagccaga tctgttcttt
    24001 gcagtagact gctctgcagc agatgacaca gtgacgaaaa atggcacatt cggacattta
    24061 ggagaaggta cattgatgcg tatccaagat ccaggtttga tcatgcttcc tcgtttgcgt
    24121 gagtacttgc tagatatcgc tgaaacaaac aacatccctt atcaatactt tgtttcaaaa
    24181 ggcggtacag atgcaggtgc tgctcataca caaaatgaag gaatcccaag tacagtgatt
    24241 ggcgtagtgg gacgttatat ccatacgcat caaacgatgt tcagtattcg cgattttgaa
    24301 gctgcacgag aaatgttgat tcaaacattg aaaggtttag ataaatcaac agtgaacacg
    24361 atcgtatacg ggaaataacg cgatagtgaa actataaaat ctggcaaaag caaccttttg
    24421 tcagattttt tttataaaaa gaggatcaat gtttgttagt tcgaaagaaa ggtgtataat
    24481 ttaattgaaa aatacataaa cagaaagtag aggaaaaaga atgattatcc caaatagttt
    24541 agaagaatta gcaggctacg tagaaactgg aaaaagtgta tttttcttta ctgccggctg
    24601 gtgcggagac tgccgtttta tcaaaccgca aatgcctgaa atcgaaaatg acttttctga
    24661 ttggcaattt attgaagtag accgtgacaa gtacatagat gtagccgctg aatggaacat
    24721 ttttggtatc ccaagttttg tcgtgatcca agacggaaaa gaattaggtc gtctagtgaa
    24781 taaagatcgt aaaacaaaac aggaaatcga atcgttccta gaaagtgtaa taaaaggctg
    24841 acatctgtta ggaggggtca agatgaatca agaatataag aacattttag taggtattga
    24901 cggtagtgaa caagcacttg ctgctttcaa aaaagcgtta gaagtggctc gccgtaataa
    24961 tgggaaagta tatgtagcaa acgtaatcga tcagcaattt tataatttta tgggctatgc
    25021 tcctattgat caaaatctag tcgatcagca aactgaaaca gccaagcaaa tgatcgaaga
    25081 atgtaaagaa tatggaaaat ctgttgatta cacaaatata gaaggaatta ttgcatacgg
    25141 ctctgttcgt gaatccatgg cagtcaaatt gccaaaaaaa tatgcaatcg atctaatcat
    25201 ggttgggcag tctggactaa atgcagtcga acgctttatg acaggaagtg tagccagcta
    25261 cgtcatacgt cattcacctt gtgatgtctt gatcgtttct gacgaagaag aacagccaag
    25321 taattaagca agtggaactt gtataacctt tttaatcaaa cagtaaaggg actgtgacaa
    25381 aagtcttgtc acagtccctt gttacgaata aatggtgttc cagaagaagc ttcttcggaa
    25441 ataaagcatg aaaaaaatct catgcagtga atgcaatttt ttgttaagat aaatctcagc
    25501 aaaggataat ggaggaaaga acatgatttt tgcttataac aaagcacacg tcggagacac
    25561 tttgatggtt atcatagccg acgataaagg aacagaaaac actgtcgaac gtaaatggaa
    25621 tgttgctcgg atcaaagatg aacaaggtca agttgttgct tggaactttt tccatatctc
    25681 cgatcatcta acaattgaag ggaacggcca agtaagctta acagatgaag aattagcgaa
    25741 attgaatcga ttgattaaag aagcaggatt cgaagaacag cttgaagctg atcatgaacc
    25801 gaagatcgtt gtcgggtatg taaaaacatg cgtcccgcat cctgactctg accatttatc
    25861 gattactgag acagaagtgg acaatgggac agtattgcaa attgtttgcg gtgcaccaaa
    25921 tatcgaagct ggacaaaaag ttgtcgttgc aaaaccagga gccatgatgc cagatggaat
    25981 gatgatctgg ccaggaactt tgcgaggggt agccagctat ggaatgattt gttctgctcg
    26041 tgaattgcat ttgccaaatg ctccagaaaa aaaaggaatc ttagtattgc ctgaggatgc
    26101 cgtagtcgga gaagtatttc caaaataaag aggttgtgac aagtctttgt cacaacctct
    26161 ttattattaa ttttcactat tttgattttg ttttaatgcc gattgatcga tcgtcaattc
    26221 aacagtagtt gttttttctt tcgagccttc atagtaagtg attttgacag aatctcccac
    26281 ttttttctta tacaaagcag attgaagttc tacaccagaa gaaacttctt tgtcatcgat
    26341 cttagtaatg acgtcgtatt gtttcaaccc agctttttct gccggtgtag ctgtttggac
    26401 atttgtcacg atgacaccgt tagttacaga tgaaggaatc ttcaagattt gttcttgttg
    26461 ctgagtagaa acggcagata agtcgaccat tgtgatacct agagctggac gtgtcacttt
    26521 tccgtctttt tccagttggt tgatgatatt cactacatcg ttacttggga tggcaaatcc
    26581 cattccttct acgcttacat tagaagtcga ttcagaagta cttgcgatct tacttgagtt
    26641 gataccgata acttgtcctt cgatattcac taaaggacca ccagaatttc ccgggttgat
    26701 cgctgcatct gtttggatcg cattgatatt gactgtttcg ttcgattcat tcgtacttgt
    26761 tacttgtcgg ttcaaggaag aaatgattcc tgaagtgaca gaattcgcat attcagatcc
    26821 taatggggaa ccaatcgcaa tcgcaggttc accaaccttc aaagcacttg agtccccaaa
    26881 agaggcaacc gtttcgactt tgtctgaatt gatcttcaac accgctaaat ctgaataggc
    26941 atctgttcca actaattcag cttttacctt cgtaccgtct ttcatcatga cttccaaacc
    27001 ttgctgtcca tctactacgt gattgtttgt gactacataa gcagtattcc cgtctttttt
    27061 gtagattacg ccgcttcctt cactataagc ttccaaatta ctatcatctg agctgctact
    27121 ttcttcttgc tgcccgaata atccgccaaa accgcttgat tggttttgac tttgtaagtt
    27181 gataacagaa acgacagcgt cttgaacttt atctaccgct ttggtgatat cagaatctac
    27241 attgactttg acattttcca caactgtttc accggcagaa ttttgattgc cgcttgtagc
    27301 agtagaacta ttgttgccag tgcccatcgc tgcataaaaa atgccagctg tcagtagtcc
    27361 tccaacgatc cctccaacaa gccccagacc caacttacgc cagaggctat tatttttatt
    27421 ttttttcatc tttggtgtca cattttttct atccatgtgt tcctcctcca attacattca
    27481 tttatctact gttagtatag tcttatttgt tgactttagt ctactttgag actttgaact
    27541 ttttatgaaa aactttagga aaaatcattg ttttcagatg aaaatatttg attttctttt
    27601 tccacagata ttgtgtaaaa ctacgcggat aggattggaa aaataaggga taaaacatat
    27661 ccacagcctg tgtataaagt ggataaattc ttataaaacg tatgtttgcc taatgaatgt
    27721 gatgaaataa ggtaaaacaa cgctgttttc tgtggataaa cctgtggatg ttgtgcataa
    27781 ctgttgggga aatcttttta acaaaaatag tcaaaataaa catatagacg aaaaaattga
    27841 ttataataaa caaagtgagc gtgaagatca aaggagagaa aacatgaata tcaaaattat
    27901 ttcagtagga aaattaaaag aaaaatactt gatccaaggt atcaatgagt atgtcaaacg
    27961 gctgaatgcc tatgcgaaaa tcgaattgat cgaagttcct gacgaaaaag ccccggagaa
    28021 tttaagcgaa gcgcaaatga gacaagtaaa agaaaaagaa ggagaacgga tcttagcgaa
    28081 aatcaaagaa caagaatacg tctatggctt agcaattgaa gggaaaaatc ccacaagcga
    28141 atcttttgct aaacaaatcg accaattagg tatccaagga aaaagccacc ttgtttttgt
    28201 tatcggcggt tctttaggac tcagcgaagc cgtcatgaaa agaagcaatg ctcagatctc
    28261 ttttggcaaa atgacgtatc cacatcaatt gatgcgcctg atcctcgtcg agcaaatcta
    28321 ccgtgccttc cggatcaacg cgggggctcc ataccacaaa taatgatatt ttttaagaga
    28381 aaaatagcat aaaaatctag ttatccgcat aaaaactgga cttatcacac tttatcaagg
    28441 tcaaaaccac tcaatttact actaatttac tacttatgaa tgagctttga tacgacgatt
    28501 tatccttgaa aagtgaagat ataaagatac ttccaataaa atttgaatat ttaataggta
    28561 gacacttcaa aaaatgaggt gtctattttt ttacccgatt ttgaaaggaa gtgaacttat
    28621 gaaaacaaaa aatcaagaat caaaaggtcg ttccccactc tttaagacca tcaaacattc
    28681 attcagccaa taaaaaagaa aggataggta aaaatatgga acttaaattt gtgattccca
    28741 acatggaaaa aacattcggc aatttagaat ttgctggcga ggataaagtc gttcagcgaa
    28801 gaatcaacgg acggctaact gtcttatcaa gaagctataa tctctattct gatgttcaaa
    28861 gagcagatga tattgtggtg gtgcttcctg ctgaagctgg cgaaaaacat ttcggctttg
    28921 aggaacgtgt gaagttagtc aatccacgta ttaccgcaga gggctacaaa atcggcactc
    28981 gtggttttac aaattacctt ttacatgctg acgacatgat aaaagaataa agaaagagag
    29041 gaaaaatgat gagattagca aatggcattg tattagataa agacacgact tttggagaat
    29101 tgaaattctc tgctctacgt cgtgaagtga gaatccaaaa tgaagacggg tcggtttcag
    29161 atgaaatcaa ggaacgtacc tatgacttaa aatccaaagg acaaggacgc atgattcaag
    29221 taagtattcc tgccagcgtg cctttgaaag agtttgatta taacgcacgg gtggaactta
    29281 tcaatcccat tgcggacacc gttgctactg ccacctatca aggagcagat gttgactggt
    29341 atatcaaggc agacgatatt gtgctgacaa aggattctag ttcattcaaa gctcaaccac
    29401 aagcaaagaa agaaccgaca caagacaaat agtcgctagg tagaaaggag actttttcgc
    29461 atgaaacagc gtggtaaaag gattcgccca tctggtaaag atttagtctt tcattttacg
    29521 atagcgtcac tcctgcctgt tttcctgctg gttgtcggac tgtttcatgt gaagacaatc
    29581 cagcagatca actggcagga ttttaaccta tcacaagcag ataagattga cattccctat
    29641 ttaattatca gtttcagtgt cgcaattctt atctgcttgc tggtagcgtt tgtattcaaa
    29701 cgggttcgct atgatacggt taaacaactt taccaccgtc aaaaactggc aaagatgata
    29761 cttgaaaaca agtggtatga atctgaacag gtcaaaacag agggtttctt taaagatagt
    29821 gctggtcgta caaaggaaaa gataacctac ttccctaaaa tgtattatcg acttaaaaat
    29881 ggcttgatac agatacgggt ggaaatcacg ctgggaaaat atcaagacca actcttacac
    29941 ttggaaaaga aattagagag tggcttgtac tgtgagctga cggataaaga gttaaaggat
    30001 tcctatgtgg aatatacttt gctctatgac accatagcca gtcgtatttc tattgatgaa
    30061 gtagaagcta aagatggtaa acttcgctta atgaaaaacg tatggtggga atatgataag
    30121 ctccctcata tgttgattgc tggtggtaca ggtggcggta aaacttactt tatactgaca
    30181 ctgattgaag ccttgcttca tacagattca aaactgtata ttcttgaccc gaaaaatgct
    30241 gaccttgcgg acttaggttc tgtgatggca aatgtctact atagaaaaga agacttgctt
    30301 tcttgcattg aaacattcta tgaagaaatg atgaaacgta gtgaggaaat gaagcagatg
    30361 aagaactata agactggcaa aaattatgct tacttaggtc tcccggcaca cttcttaatc
    30421 tttgatgaat acgtcgcttt catggaaatg ctgggaacaa aagaaaacac cgcagttatg
    30481 aataagctga aacagattgt catgttaggt cgtcaagctg gcttctttct aatactggct
    30541 tgtcaacgtc cagacgcaaa atatttaggc gacggaatcc gtgatca
//
LOCUS       DACKAC010000029        30465 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.029, whole genome shotgun sequence.
ACCESSION   DACKAC010000029 DACKAC010000000
VERSION     DACKAC010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.029"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..554)
                     /locus_tag="I0B46_001374"
     CDS             complement(<1..554)
                     /locus_tag="I0B46_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAR2083684.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(680..2608)
                     /locus_tag="I0B46_001375"
     CDS             complement(680..2608)
                     /locus_tag="I0B46_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAR2083685.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(2914..3630)
                     /locus_tag="I0B46_001376"
     CDS             complement(2914..3630)
                     /locus_tag="I0B46_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAR2083686.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(3631..5454)
                     /gene="glpO"
                     /locus_tag="I0B46_001377"
     CDS             complement(3631..5454)
                     /gene="glpO"
                     /locus_tag="I0B46_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAR2083687.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(5468..6964)
                     /gene="glpK"
                     /locus_tag="I0B46_001378"
     CDS             complement(5468..6964)
                     /gene="glpK"
                     /locus_tag="I0B46_001378"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAR2083688.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(7100..8560)
                     /locus_tag="I0B46_001379"
     CDS             complement(7100..8560)
                     /locus_tag="I0B46_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAR2083689.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSSFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(8550..9845)
                     /locus_tag="I0B46_001380"
     CDS             complement(8550..9845)
                     /locus_tag="I0B46_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAR2083690.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVKEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(10177..10854)
                     /locus_tag="I0B46_001381"
     CDS             complement(10177..10854)
                     /locus_tag="I0B46_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAR2083691.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(10884..11975)
                     /locus_tag="I0B46_001382"
     CDS             complement(10884..11975)
                     /locus_tag="I0B46_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAR2083692.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(12076..12510)
                     /locus_tag="I0B46_001383"
     CDS             complement(12076..12510)
                     /locus_tag="I0B46_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAR2083693.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(13094..14551)
                     /gene="ascB"
                     /locus_tag="I0B46_001384"
     CDS             complement(13094..14551)
                     /gene="ascB"
                     /locus_tag="I0B46_001384"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAR2083694.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(14548..16443)
                     /locus_tag="I0B46_001385"
     CDS             complement(14548..16443)
                     /locus_tag="I0B46_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAR2083695.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDPVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(16501..18276)
                     /locus_tag="I0B46_001386"
     CDS             complement(16501..18276)
                     /locus_tag="I0B46_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAR2083696.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(18326..19822)
                     /locus_tag="I0B46_001387"
     CDS             complement(18326..19822)
                     /locus_tag="I0B46_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAR2083697.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(20016..21638)
                     /locus_tag="I0B46_001388"
     CDS             complement(20016..21638)
                     /locus_tag="I0B46_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAR2083698.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(21966..23279)
                     /locus_tag="I0B46_001389"
     CDS             complement(21966..23279)
                     /locus_tag="I0B46_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAR2083699.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(23524..23748)
                     /locus_tag="I0B46_001390"
     CDS             complement(23524..23748)
                     /locus_tag="I0B46_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083700.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(23768..24136)
                     /locus_tag="I0B46_001391"
     CDS             complement(23768..24136)
                     /locus_tag="I0B46_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAR2083701.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(24332..25042)
                     /locus_tag="I0B46_001392"
     CDS             complement(24332..25042)
                     /locus_tag="I0B46_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083702.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(25060..25557)
                     /locus_tag="I0B46_001393"
     CDS             complement(25060..25557)
                     /locus_tag="I0B46_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAR2083703.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(25594..26046)
                     /locus_tag="I0B46_001394"
     CDS             complement(25594..26046)
                     /locus_tag="I0B46_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAR2083704.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(26181..26786)
                     /locus_tag="I0B46_001395"
     CDS             complement(26181..26786)
                     /locus_tag="I0B46_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAR2083705.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(27236..27856)
                     /locus_tag="I0B46_001396"
     CDS             complement(27236..27856)
                     /locus_tag="I0B46_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAR2083706.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(27858..28727)
                     /locus_tag="I0B46_001397"
     CDS             complement(27858..28727)
                     /locus_tag="I0B46_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAR2083707.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(28750..29157)
                     /locus_tag="I0B46_001398"
     CDS             complement(28750..29157)
                     /locus_tag="I0B46_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAR2083708.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(29176..30465)
                     /locus_tag="I0B46_001399"
     CDS             complement(29176..30465)
                     /locus_tag="I0B46_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAR2083709.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
BASE COUNT         8632 a         6627 c         5021 g        10185 t
ORIGIN      
        1 tattttcttt gaaacatctt tcggtactcg acagcataca agacaaaaga ttgtaaaaga
       61 ccgtaagtga tgacggcaaa aatgactctc acccacagcg ttggtaattc tgggaaaagc
      121 agcaaaagga tcatgccaac atagatgaca gcagtcgcta ctttgccata ccacttcgct
      181 ccacttaatt tgatattttt agatagcccg attaatccag ctacaaacat aaagccttct
      241 tttatcaaga atagaccaaa caacagccaa agccacggtc gataccatgc catagacaaa
      301 atcagaaaaa gttgtgtcaa tttatctgca aacggatcaa gcaccttccc taagtctgtt
      361 acctgattat atctccgagc aatgaccccg tctagataat cagacaaacc tgagacaaca
      421 atgaccgctc ctgctatata aaatgactgt ctcagcacaa aataaagata aatcggcagc
      481 aaaaaaatac ggaatatcga taaaagattc ggaatcgttt gtatttcttt acgccagtct
      541 gtgcttaatt tcattttatc gccctacctc ctcagcactc ttttcttttt attttagctt
      601 acacggggaa taaaaccaat gatttcactg tttttatttt tctttacagc cactgatatt
      661 atgagctaat ttgagataat cacttgctgt tttcatcgtt tactcttctt tcttcctgtc
      721 gtttgcgcat ttcttttact agtactttat gagctttttt tgatcctcgc tctaggtcac
      781 gctgccgttt acgtgtttta taatcgataa acagactgtc taaatcgtaa cctgtcgggt
      841 acagatcttc tgctgaacgc accagacgaa cacgctgacg aggcacgtgg attatttttt
      901 tgtctaaata tacatcaatc gtatcttcac ctatatcttc ataaactaac gcttcttttt
      961 ggtattcatt gacaaatatc cgatcacctt ttgcaaagag ctggcgttct tttttattct
     1021 ccttcctatt tgtaactgct gtcttaggaa gaaattcttt tttctgtgtc cgataggctt
     1081 tttccgccaa gtaacgctct gcttgatgga tcaatttcat cgacatatcc attttatgag
     1141 caatccacaa agcttggctt tctcctgttt ctccaacccg caactgatat ttaggacgaa
     1201 gtgcttcccg atcgaaagcc atagcagccg gcacaaaatc ttcatgatct ccggcgaatt
     1261 tcttgatttc tccataatgg gtcgtggcaa tgatcaatgc cccttgctca tacatagatt
     1321 ccatgattgc gatcgctagt gcagcccctt catttggctc tgttccactg ccaatctcat
     1381 ctaaaagtac caacgtattc ctttttatct ttttcaaaat agcagcgatg ttttgcatat
     1441 gaccagaaaa tgtactcaac gcattttcta aattttggtg gtcaccgaca tcgacgaaaa
     1501 tctcatcaaa gacagcaagc tctgtccctt cttttgccgg aacttgcata ccgaacatcg
     1561 ccatcaaagt caataaaccg actgtcttca acacgactgt tttgcctccg gcatttgctc
     1621 cggtgataat caagcctcga tagtctttac cgatttcaac atccaatggt accgcatgct
     1681 caggtaaaaa tggatgtctg ccttgcttga taatgatatg ttccgattta ttgattctag
     1741 gtgtgcttcc attgatttct cggctaaatt ttgcccgagc aaagatgata tctaacaccg
     1801 tgatcgtttc gatcaataaa tcgatcactt cttcgttttc tgcgatagca ccagtcaatg
     1861 ccgccaggac ttgatattct tcactgattt cttcagcttt taacaactga tagtgttcat
     1921 tcaatttaga aatcacagtt ggttcaataa aaaccgtcgt tcttttattt gattcatcga
     1981 taatcgttcc atctatttta tttttatagc tggctttgat aggaatcgta tagtattcgc
     2041 ctttttgaac aatcattgct tcttggatca tttctttgtt tttcggatga cgcaagaatt
     2101 tcagtaattt tgactggatt tctttttcga ttagttgtaa ttgtttgcgt actttacgca
     2161 aattacgtga cgcgtcgtca ctgactttat tattttttat tttctggtca atcaactctt
     2221 ctacatttat caaatcagga agatgtttgc tataagcaaa caacaagggt gtctggtatt
     2281 gattcttatc aaaaaacttt gtgatcatcc gactgcttct aagaaaatcc gcatattcga
     2341 tcaaatcggt tggctgcaga atcaaccctt ttttgacttg ttctgtcagt gcatcgatcc
     2401 tcggcaaccc cataaatgga acatgctgac tgctttctaa gatcaagcgt gcttctcttg
     2461 tttcttcttg acgatccaag acagtttgta ggtttgtcga gaccgtcata tcgctgattc
     2521 gttttttgct atagtttcca attgctcttg cttggatttc ctttttgatc cgatcaaatt
     2581 gtaattgact aatcatttcg ttgtccatat tctctccttt tcttgaagag atacctgtcc
     2641 caaaataaat aagggcctag agataaataa aaggatagaa cacagcttaa aaaagtcatg
     2701 tcctatcctt attagaatac aaagatattg ttttcctgac aggtaccgtt tcataagcac
     2761 accaaataaa ctttgtttat tggctattcg acttactgat tagataccag ctacactcac
     2821 aaaacatacc ccattcttct aggtcttatt tattttccac ttaactatag caagacacat
     2881 tttggttgtc aaactttagt atgttttata gatttacacc attaatgaat agacacctac
     2941 cgcgataatt gctccgatca ttggacctgc gattggtacc catgaatatg cccaatctga
     3001 agttccttta ttagctattg gcaataccgc atgtgctaat cgaggaccaa agtcccgagc
     3061 agggttgatt gcatatcctg ttggccctcc taatgaaaga ccgatggcta agatcaatcc
     3121 acctacagcg aatacgttcg ttccaggtgc aaattcgttt tgtccaaaag ctagcaaccc
     3181 aagaactaat acaaatgtac caattaattc tgtgatcaca tttgctggat aattccgtat
     3241 agcaggacct gttgcaaacg ttcctaaaat cgcgctttca tctttcgttg cattccaatg
     3301 tggcaaataa gaaagccaaa caagaatggc ccctgcaaaa gctcctaaaa cttgcgcaat
     3361 aatgaaagga acgacaagat tccaactaat tgcccctgtc atcgccatag ctagagagac
     3421 tgcaggattc aaatgagctg ggcccatgaa tccagaaata tagacagcca ttgttacagc
     3481 gagtccccaa ccaaatgcga tgacaatcca gccagaagcc tgtgctttac ttttgtttaa
     3541 attcacagcc gcacacacac catctccaag caaaactagg atcagtgtcc caataaactc
     3601 ccccatgatt tgagtcatat cactgttcat ttatttctcc ccctttagtt cccgcagatc
     3661 tgattctgca atcaatgctt ctaaacgttt agtctgttgc gccttctcct cttctgtcca
     3721 ttcaaaatat ccagcaattg catcgatcac aggttgcttg atctcatcaa gctgatctcg
     3781 ttcaaacaac agatgatttg tacgacgaat gagataatcg cctggggcca aaaccatttc
     3841 ttcttctaaa ccataacgta accgagctga ctcagcgaga ctcaaaccag gataaggtgc
     3901 catttctttt gctaattcaa agatacgtcg ggcattagtc ccgtaaaaat cagcgatata
     3961 agtagcatct tcttcctcta aaccggctgc tactccttct tttgctaatt ctgtcactgt
     4021 ttcttctaat ttagttggat cgaagtttcc acctgaaatc tgatattttt tagagtcgat
     4081 ttctttcgtc tctattccgt attcttcttt taataattgg cgtatcaatc gcaaagctcc
     4141 ttctgccatc ttacggtaat cggtgatttt cccaccagat aaagtcagca aaccatctgg
     4201 ttctctttct aaagaactac ctctggaaat cgtagaaggt gctttttcat cacggctgtt
     4261 ttccaaatgg ttcaacacgt cttctacttc agcacgagaa actttgtttt ccttatattc
     4321 acttactgta tcgacaactt tattgaagct cttgtctgaa atcgatccat tatctccacc
     4381 attgtaatca gaaccagaat taccaatcaa cagtggacga agtcctgccc agcttgcttc
     4441 gatatcattc aatgtgatat ttgcttctgg atagcgatga ttgatcacat ccaatagata
     4501 atctacatct tcttgtgtga ctttcggatc cgtaaaatct ccttggtaat ccgtatctgt
     4561 cgtaccaaag taagtcttgt tttctcttgg aatagcaaaa accatccgcc catcttgctt
     4621 tcctgtatcg aagtatgtgg gttgcggtac aggcagtttt ttcgcatcta caactaaatg
     4681 gatcccttta gttggacgca ttttaggaga gactggacgc gtaaaattca agtttcgtac
     4741 tttatctacc caaggaccac tcgtattgat cactaatttt gctttgattt cgatcacttc
     4801 atctgtcagc aggtcacgag ctttgacgcc aacgatttga tcgccatcat ataaaaagcc
     4861 aaccgctttc attttactta ctagataagc cccatcttcg gcagcctttt tgatattatc
     4921 aatcactaaa cgggcatcat tgttgcggaa atctagatag acacccgcgc cttttagccc
     4981 ttcttttttc aaaaacggtt ctctttccaa tacttcttct ggtgtcagcg tataattctc
     5041 atatttggtt cctgtcacat ttgccagttt gtcataaagg tccattgcta ctttgaccga
     5101 gaacatattg aaggttgttg ctccttcgcc ctcatagatt ggcaaaagca ttggatctgg
     5161 ttttgggatg tgtggggcaa tactttgtac gactgcacgt tcaccaactg tgtcagctac
     5221 tacttccaca tcaaatgtct tcagataacg aataccgcca tgaacaagtt tggtcgagcg
     5281 agaggaagtc ccttctgcaa aatcctgcat ttcgatcaat cctgttttga ttcctgatgc
     5341 tgctgactgg actgccacac cggcaccagt gattccgcca ccgatgatca acagatccaa
     5401 ttcttcttgc tgcatttttt gaatgctatc ttgtcttgtc ttgtttgaaa acatatggat
     5461 ttcctcctta ttcaaatgct tgggtagctt ttactgcttt tttccaaccg ttgtaaagtt
     5521 tttctcggcg ctcttcctcc atttgaactt caaatagctt gccagcttca taaaattcgc
     5581 ggatctcgtc tgtatctttc caataaccga cagctaatcc tgctaaaaat gctgccccta
     5641 gcgccgttgt ttccaaattt tctgctcgtt ggatcggtac attcaagata tctgtttgga
     5701 attgcatcag atattcattg tttgctgcac cgccatctac tttcaataca ggaatcttga
     5761 ttccagtgtc atcttgcatc gtatcgatga tatctctcac ttgataagca atcgactgta
     5821 aagtagcttt aatgatatct tcttttgtcg taccacgtgt caagccaaac atagcccctc
     5881 gtgctgcctg atcccaataa ggagcaccta gcccaacgaa tgcaggaaca acataaacct
     5941 catcttcgct tgtagagcgt ttcgctgcgt cttctgaatc gctagctttt tgcaacattt
     6001 gaagtccatc tctcagccat tgaatcgaag aaccagccac aaaaatactt ccttctaaag
     6061 catagtaaac ctttccattt atgccgtaac caattgtcgt caataaatta tttttggata
     6121 attgcggttt ttcaccggta ttcatgacaa taaatgaacc tgtaccataa gtattcttga
     6181 ccatgcctgg ttcgaatgcc atttgtccga ataatgcggc ttgctggtct cctgccatcc
     6241 ctgaaattgg tgcttcgctt ccataaaaat gataaccttg cgtataacca taaacttctg
     6301 agttagaagt gacttttggt aacatcactc gaggaatatt tagtaaatct aaaatctcct
     6361 gatcccaatc tagctgatgg atattgaaca acatcgtccg actggcattc gagtaatcag
     6421 taacatgtgc ttgtccatcg gtcagtttcc atactaacca agaatcgatc gttccaaaca
     6481 tcaattcgcc tttttcagct cgttcttgcg cgccttctac atgatcaaga atccagcgta
     6541 tcttcgttgc tgagaaatag gcatcgatca ctaaccctgt tttttcgtgg aaaaattctt
     6601 gatagccttc attttttaag ttatctgcaa ttccagctga ctgtctggat tgccaaacaa
     6661 ctgcatgata gattggacgt cctgtatctt tttcccatat tactgttgtt tcacgctggt
     6721 tcgttatgcc aatcccagca atctgatttg gtttgatccc tgattcgatg aacgctccgg
     6781 caataactga ctgtacagaa ttccagattt catttgcatc atgttccacc caactttctt
     6841 gcgggaaata ttgcgtgaac tctttttgag aactgccgat gtgtcttcct ttcttgtcaa
     6901 agataattgc tcgtgagcta gttgtacctt ggtcaatcgc catgatatac gttgattctg
     6961 tcattgtgat tccctccatt atatgcaaac gctttcttta actactctta tcatactact
     7021 gttttaaaaa aagaaactgt caaaatttta ttcttttttt ctttttttga cagtttttta
     7081 ttatgaagct attttttaat caattctttt cttttttcag cgtctctatt cttcttttca
     7141 atcgaataat gtcgtaaggt gacgcaaatt ctgacagata atattcacac tgttttttgt
     7201 ttctctggca aaatgtgatc accaaatcat attctttacc ttcttcataa cttccaatct
     7261 caataccgcc gattcccctc agttctctga tcagatattg ttgaaaagca atacgataaa
     7321 tcggcagact ttctaaatcg attccaatca ctactggttt tgcaatataa aaatcgatca
     7381 tcaaaagaac attggcatac tggatcatca ggtaactaag ctcactatct tcagctcttt
     7441 tcctcggcaa ttgctgcacc gctgtttcct gtaatttcgc cagtaactgt gttaaggaat
     7501 gcccgagcat tttctgctgc tgttccaaaa gccgatcacg ctcatagact tcgatctttc
     7561 caacaaagaa aaaccattca ttgttgatct gggaaatttg gtagcctact gccttttctt
     7621 ctttgatttt caaacgacga ggacgataat gccataacat cgtttcccga atgtaagtat
     7681 ctaaaacagc ggttggtaac tttttgctcc gtgtcaaatc atactgataa aatatttctt
     7741 cgtctactat cgaaaaagaa acaaaagagc tgtaaaacat cattgtttcg tatacattca
     7801 gttctgctgc tgttcgacta agatgcatca cgatgattgg atcgattgct tgataaagcc
     7861 ggtccgactg atagagtaat ttcttttctt tcaaagttgc gtacgtactt ttttcattca
     7921 atagtctttt tttcgtaatc ccgagccagc aagcaatctg tgcttctgct gatgccgtaa
     7981 acgttgtttc taatacacgg tccaatccta gaatgaacgg tcttttttct ggtgtatttt
     8041 gcaaaaaaag cggtcgctga tcttccggga tatattggaa tagttcataa taaaaatatc
     8101 gtatctgtag ctcttctccg tataattgac cgtttttgat ttgtaatcca aattcctcta
     8161 agagctggtt caattctttg atctttcgaa aaacagaaga ctcactgata ttaaacgctg
     8221 tcgctaatcg aacgatggat acttgctgat gttctaataa atagacgagc atcttaaact
     8281 tcaagctttc ctgcaataaa aacaatagaa ttttttccaa acttacatct tcgtccaaca
     8341 caagcagcgc ctgatactca tttcgctgta cttctacttt tttttccatc attcggccaa
     8401 tcaattcaat atcttccaaa tattgatcaa acgaggcttt tgacagttgg acatgctctc
     8461 tcaaaccatt gatagaggct gttccccctt gcagaacaag gtttcgcaaa atcaggatct
     8521 gtaactgctc ttttctttct aataatgctt caatcttcat aggcagtatt cgccaccttt
     8581 gaccacacca tatctggcac acggtattct ggctggaaaa atagctcatg ttcttctaac
     8641 tgttcaaacg tccatccttc ttttactgca attgtcagtt ggtcaagaag gtggaacatc
     8701 tcttttttgc tttggagctg tgctccaaga atcttttttt cagaatcaaa tagaaatttg
     8761 agtttaactt tgctttgatt tattgctgta agcttgtagt ctacgtatcc tgtttcaatt
     8821 ttttggggat aaaaaatgct ctcttcttct gtcaaaccta cgcttcctaa gaaatagcca
     8881 aaccactgat tgccgactgg acgataggtt ccctgatcct ttgtctgtga gccactaatt
     8941 gtcttgctga catttcgtgc tgtacggaca gcgttattta ctagagaaac atacattttt
     9001 tcatttgttg gacgaaaagt tacttggatc gcatcgccaa tcgcataaac gtctttttga
     9061 gacgtttgca agtagtcatc tacccagatc gttccatcat cattcaatgt taattgttct
     9121 tcccacatct gattgtcggg gcgagaatga ttagccagca aaacaagatc atagttctcc
     9181 tttctttttt ccgtctctac aatagcggtt tcattttcgg tcaaagacac aacttgttct
     9241 tctaaaaaaa gtgaaagccc ttgatttttc atttccttta ctaacggttc tgtcatttca
     9301 ggatctagat aacgaaaaag aattgacttt cggctttcgt aaagatggat ttgttttttt
     9361 cttgatgcca acccttcagc tatttcgatt cctgcttgtc ctgcaccaat aattgcaatt
     9421 ttctttgcat tcatgatctt ttgctgaaga gccgttacgt tttgtatctt tttaactggg
     9481 tgaataagcg ttgattctgc agaaatattt cgaaactttt gatttgaacc agtcgctaga
     9541 atcaaacgat caaaatccaa tttttttcct cctgctaaaa cgactttttt ttctttcaat
     9601 cctattactg tcttttctgt gtaaacatgg attcgatagc ggtcagtcag ttcttctttc
     9661 gtgatccaag aaagttgttt ttcactggta acctttcctt ttaatatcaa attgaacccg
     9721 ctgggaatcc atcctaactt cgattgcttt tcaatcaaat atatttcact tgttggcatc
     9781 aattggcgaa gataaatagc cgcatgaatc cctgcaaacg acccgccgac aattacaatg
     9841 ttcatctgca ttcctctttc ttataaggtg ttctactgct aacttctcaa tcagaaaaca
     9901 cagtattcat aactcgcttt caaccttatg atacatagcg atgcagcaga atcaaccctc
     9961 tccatcatac cgtgttctag gtctgcttct caataatgta ctagtggacc taattgaaga
    10021 gtatcatttc tgaagagatg tttcaaaaaa tgtactctta taaaagaaaa aaactcgcct
    10081 tttacgtacc tctgtccaaa taggaaacag aaaagaacga aaagacgagt tttagcgtgt
    10141 gtaatgtaat agttagaata gacacatcat ttatttttaa ttctttaatg cttcaacaac
    10201 tgcttcgcct aattgatgag cagattgagg attttgccct gtaagaagcc gcccatcaat
    10261 ttgaaccact tctgaaaaag ctgctccaga ctgatagttt gctccttttt ctttcaaagc
    10321 atcttctgct aagaatggta cttcattcgt tgtaccattt gcttcctctt cttcattact
    10381 gaacccagtc actgtacgtc cagctaaaat agattttcca gactcatctg taactggcaa
    10441 caatcctaca acaccgtgac aaacagctga gaccagccct tcatttcgat agatttgtga
    10501 agcgactttc ccaagatcta ttgcttctgg aaaatcccac attgttccgt ggccaccagc
    10561 ataataaacc gctgcataat catttgcatc cacatcatca ggacgtaagg tatttcccaa
    10621 ggcccgattt ctgaaatctt catcttcata ataggcccaa tcgatttcat ccatattcaa
    10681 aacactttgc ggttctaaag gtacatatcc gcctttagga ctgacaaaat ctgcatcgat
    10741 cccatttttt actaatacat cataaaaatg agtcagttca cttaaccaca aacctgtagg
    10801 acgctcgatc ttttcgtact tttctacatt tgttaatacg atcaatactt tcattttccc
    10861 atcctctttc tataaaaatt gcgttattga taacgtgctt tcaagaaatt atataatggg
    10921 cttggcaaat aaagctcttt atatgcttct tcaaaaggtt tcgttacaaa ttcatcctca
    10981 cggttttcca ataatttctc tgcccagtca ggatcgacga tcatttgctg tccaattgct
    11041 gcaagttctg catatttcaa tacctgctct acatcttcgc gattgcggat cccaccgaca
    11101 ccaactaaag ggatctttcc ttgaatcgtg ttatggacca cttcaagaat cgatttatcg
    11161 ttgtatttat cagaacgagc tattcgatca tacatattta atgaaacatg cagataatct
    11221 agttttgact cacgtaatcg ttcaagaagc cagatcgtgt cttcaaaacg gatacctggt
    11281 gtttcatatt cttctggtga gaaacgatac cctacgatga atggtttttt agcatattgt
    11341 tcaaccgctg agaaaaccgt tgttaataat tcttcaataa aacgataacg attttccaag
    11401 ctaccgcccc attgatcctc tcgtctattc gaatgcggag agaaaaattg ttggatcaaa
    11461 tacgtatttg ctccatgcaa ctcgattcca tcaaatcctg attcaatcgc tctacgtgtg
    11521 gctttgccaa atgcttggat tgtttcaacg atttcttctt cactcattgc tcgtggtgtt
    11581 tccgcatcag gacgttttgc tgcaactgca cttgcagata caggctgttc tccagataaa
    11641 gttgctcgac ttgtcattcg tccaccgtgg aaaatctgaa taatcgcttt tgcgccttct
    11701 ccttggattg ctttcgctag ctcacttaat cgaggaatca tttcatcatg agcgatgctg
    11761 agttcacctg gccagccttt tccgatatct tggacatttg cagcaccagt aataacagca
    11821 ccaagacctt tagaacgttg tttataataa gtgatctcat cttcggtgat cactccattg
    11881 aaaaagctga gttgtgtcgt cataggcgac agcatcaatc ggttcttcaa ttgaactcct
    11941 gaaggtaaag taattgattg atcaaattgt gacattgtca ttcctcccat aattcgctct
    12001 gattaaccta tcgagaatca tctatcatta aaccactcga ttagttgtac atgcaactaa
    12061 tttgcttaaa aaattttatt ttttttgtaa cttatctgtt gcatcattca ttgtctgggc
    12121 taattcttta cttgcttcat ttcccaaaac tgctttgtat gactgatgaa gttcttccca
    12181 gctaccatcg acttccttag caaattggcg tccttgttct gtaagataaa ctaatgacaa
    12241 gcgaccttcg ctaactgttt tgacgtatcc tttatattct aatttttcaa caaaacgagc
    12301 aatcgttgat ggggcggaat agagcatttg tgcaatatct ttttgtggta ttccatcttt
    12361 ttcttttaat atcaataaaa taaaaccata agttggtgcc agacctgtcc ttctaaacgc
    12421 ttcatcagct aatttattca acatgcgatc tagctttttc acgctaaaga aaagacattc
    12481 atctaacgct ttttcaattg gcttttccat ttcatcccct ccattggcgt acatatcata
    12541 gtatgatttt tattttttgt caaaaaacac ggaataatgt ccgcctcctc tgtctagatt
    12601 tgaaaaaaag aagtagccat cttcaaagaa tttcggcttg ttttcgacaa aactccttct
    12661 ggaacaccgt gaatcagagg ttgtgaaaaa gtcgtttagt tttgagtatg aagaaaaatt
    12721 ttgtgaaaat ggctttccac atttttgcat aaatttggct taataccgaa aaactggctt
    12781 ttgaacaccg tttatatcag aggttgtgaa aaagccgtcc tgcatcaagt aataagaaca
    12841 gccaaacaaa aatagttcct catattttgt cgttttttga gctaatgccg atggtcagct
    12901 gattgtacac cgtttattga gaggtcgtga gcctgccgtt gaactccgag tcacaaggag
    12961 caatttcaaa aaacagcttc tcatgtttta tgactatggt gacttgtgat cgaggagttg
    13021 gcaggagaat actgtttatt cgtaaagaag gggctgtgac aagacttttg tcacagtccc
    13081 ttatttcctt ttattatagg atttcgccat ttgattggat gacttgtctg taccaatcaa
    13141 atgatttttt ctttaaccgt ttctttgtgc cattcccttc gtcatcttga tcaacataga
    13201 tatatccata acgttttgac atttgtgaag tagaagcgct gacgatatca atgcagcccc
    13261 acgacgtata accaaataaa tctactccat ctttgatagc ttccttcatc tgctcaatat
    13321 gctgcttcaa atagtcaatg cggtaatcgt cttgaacggt tccatcttct tctactttgt
    13381 ctatagctcc gagtccgttt tcagctatga acagtggttt ttgataccta tcatataatt
    13441 gattcaaagc aattctcaaa cccttaggat caatctgcca gccccattca cttgatggaa
    13501 gatacggatt tttaattcct ccagttatta agtttccttg gaccccttcc caattttctg
    13561 gatgagcact gactgcacta gacatgtagt agctaaaaga aacaaaatca acagggtagg
    13621 ctgctaagat ttcttcatca cctacttcta tttttaagtt gacacctttt tcttcaaaca
    13681 ttcgttgcgt atacgctgga taatatcctc ttgcttgaac atcactaaag aacaaagaag
    13741 ttctttgctg ctcaattgtt ttttgaatat catccggatc acaagaatac ggaacaagaa
    13801 gctgatagct gatcatacaa ccgacttgtg aacctttgat aatttcatga caagcttttg
    13861 tagctaaagc acttgctata aactgatggt gtgcaccttg gtatttcgct tgttccaaat
    13921 ggggatggtt ttcttcaatt actcctacgc taacaaaagg gtgatgtttt acacagttga
    13981 tctcattgaa agtcatccaa tattttactt tatttttata gcgtttgaat actgtttcag
    14041 cgaaacgaac gtaaaactga ataagctttc gattaggcca tccaccatat tctgtaacca
    14101 aatataacgg catttcataa tgggaaagag aaacgactgg ctcaatccca tattttagac
    14161 attcgtcaaa cacttgatca taaaaaaata atcccgcttc attgggtgtt tcttcatcac
    14221 catgaggaaa aattcttgtc catgcgatag acatccgata gcatttaaaa cccatttcag
    14281 caaataaacg gatgtcttct ttaaagtgat gatagaaatc atttccgtgt cttttaggat
    14341 aataatactt gctgttggga tcctgcgccg cttttacttg ctgatgcgtc atcatatgcc
    14401 attgatctgt ccatttttct ttaggtaaat cgctgtcgaa tgtatacaca tcagacacag
    14461 ataatccttt ttcatctaca tcccatgcac cttcacattg attggccgct gtcgctccgc
    14521 cccataaaaa tccttctgga aattttttca tatcgatccc cctaaacttg ctagttttct
    14581 tgtttgatta tttttcttat gactagtaat gatatttttg aatctactac tatttgtaac
    14641 aataaccatt gtggtcggat catatccctc tttgattaat tcttgcaaat ctacttccat
    14701 caataaatcg cctttacgta ctttttttcc atcagatact gcactttgaa agtatttgcc
    14761 atttaaattc actggatcaa taccgatatg gatcagtatc tctgctcctt cttcactgac
    14821 taccccaatt gcatgtttcg ttggaaaaac tgttgaaact tccccgttta cagaactgta
    14881 gatttttccg tccgttggaa tgatcgcaaa cccttctcct aaagatccac tagcaaatgt
    14941 tggatcgttt acttcagata aatcaattat ttcacctgct gcaggagtac ttaacactat
    15001 atcctttgag gattcttctt cgatttcatc cttgtagccg aaaagaaaag ttagcataca
    15061 ggaactgatc atcgctaaga aataactagc aactagccaa acaaatcctt caccaatata
    15121 aatcgggaaa gacaaaatac ttcttgtagc aactgccaaa gcaccggctc ctgcggcacc
    15181 agctactcca ccaaatatag ctccacatat acaagcaatg taaaatggtc gtttatattt
    15241 aagagtcaca ccataaacag caggttcagt gattccaaat aatgctgtga taaatgcaga
    15301 taaagataaa ctttttactt tcttattttt cgttttcaaa aataccccaa gtactgctcc
    15361 tgcttgcgca gctacggcag tcatacatgc tggacctagc gtatcacgac cgtagaaaga
    15421 aagattgttg gtcattactg gtacaagtcc ccaatgtaac ccaaaaatca ctaacacttg
    15481 ccaaaatccg ccgataacag caccagataa aacagggcta aagttataaa cagctaaata
    15541 tccagctgca atcagctgac ttgcataatc tgcaattgga cctacaacta ataaaataat
    15601 tgggaccatc acgatgatac aaatgaatgg tgtcaataaa tttcgaatac tttcgtgcaa
    15661 gcttttgcga atcttaggct caatataaga aagaacccat acagctaaaa ttgctggaat
    15721 aaccgtactt gtataccttg ctagaatcac gggcatccct agaaatcgca aggttatgct
    15781 atcactatat gcactgacaa tcgtaggata aacgagagct gctgcaataa ccatagcgat
    15841 gaaacgatct gtattgaact tttttgcagc agtatatgcc aaaaatatag gcaagaaata
    15901 gaaaaatgca tcagctgctg cattcaaaat gatataagtt cctgattcag tagttaataa
    15961 attcaaactc gtacataagg cgagtactcc tttgatcaaa ccacttcctg ccaagacgcc
    16021 taaagtcggg gcaaaaacag cggcgatcat gtctataaaa gagtttaaaa tgcctttttt
    16081 ctcagttcct ctctttccac tagatgcatc atcttctagt ttcatttgtt tggataatac
    16141 gtcatacact tcatttactg catttcctac tacgacttga aacattcctc cagcttcgac
    16201 tacagtgatc acaccttcta gttgctctac cttctcctta tctgccttct tattatcttt
    16261 tagggtaaag cgtaaccttg ttgcacaatg gtaaacattg ctgacatttt cttcgcctcc
    16321 gacaaatcgg acaatatctt tcgctaattg tgaatagtcc atttttactt cccccttgtt
    16381 aattaatgcc gtagcaatcc ttcttacaag ataagtatag ttcttagaaa acggtttctc
    16441 cattcaatta gtaccccttc taatagcgaa cgaaactgta caatagtgtg tatggtttac
    16501 ttagccgagc aaatcactca ctgcttgaaa gaaaacaagt atatcgtttc tcttatagtc
    16561 aaaggagaca aaaaaaacac gcttgatctt cctttgctga tgtataagtg gtatctcaag
    16621 ctgatactct gtgatttttg tttctacttg gcttttgata agacagcaca acaaagtctc
    16681 ggcatttaac ggaagcataa ctaggtcaga actaggagag attagttgct gaatggaatc
    16741 tatcttttta tggattttta tttgattttc ttctttgtag taatgatcaa gcagttttgt
    16801 tttctttttt tcttcaacta tggtcagctt ttcacgcaaa atctgataaa tctcccaaga
    16861 ttcaaactca tctagaacac taggtagata aatcaaatca tcccactcaa aaataagctc
    16921 ttgctcagta gtcaaaattg catcatattc cgttattttg tttgattgat ttttgaaaag
    16981 atacactggt tctatccggc attctttgat ccacatgttc atactttgtt gaatctgttt
    17041 ttttagtgta ttcaaaatac taattggctg gttactaaca atcagtatct ccccttgttt
    17101 tcgaaaagga ttcaacgctt cggcaagata caaaacaaaa taagcgcttt cagctaaatt
    17161 gggacgtaat tgaaaatgta ttgggaagaa acgccgaatt aaataaattt ctaaaggata
    17221 acgggagatg atcttttgta caaaatgatt catggtatta tgtccggcat ccatcctcgt
    17281 tttcatttgt ttgatatggg ttagcaaaaa ttctgtttca tggaacaata acgaaacagg
    17341 aagatctaaa aaacgaataa tcgtttgctc aaatgctctt agattcttct ctatttcttt
    17401 gtttttttca tcattaatca tcaaataatt taactctaat agtcgatttt caattgcttg
    17461 tttttcttga ttggaaaaag aatagggtag ttctgatgat aacttttttg tgagggtctc
    17521 taccatcgga ataaataatg tatacctgtt tgttttttct aaaagttctt ctcttcgaag
    17581 aatggtcaaa acgagatacc gagaaaatcg tatgaagtcc tctcctgaaa caatataatt
    17641 atatgatata agaacctctt ttaatgtctt ttggacagtc ggcatgatat tggaaaaaat
    17701 tgtactgatt tgttgaggaa accttgcgtg ttggatcacc gtttctgttc caacaagaga
    17761 taagaaaatt ctttttgaat tttcttcagc aatgattttt agtccttttg tcgacgaaac
    17821 ttccagttct aatttaggat tgcgctcgat ccaacgcaaa atcgttttta tttgtaatga
    17881 tacggctgtt tttgaaagaa agaaaatgtc tgctatcttt tgcatactga catacccttt
    17941 ttgaaacaat aagtacgtca caatagttga agctcggaaa tccatgctgg aatagatttt
    18001 atgtttcgtc acctcttgta tgatttgctg tttagtttca gattcaattg attctaaaaa
    18061 ataacctgaa cgtttatttg actggataat gattttcttc tcgtagatag caagcatatt
    18121 tttgatttca gtttggatcg ttttcttgtt ggtacctaaa gcttcagcta attcttcgct
    18181 tgttatccaa tgattcttat ttcttaatac tacaagaatt tggagttgtc gtttatttaa
    18241 gtccatttgc ttacacctcc tctttgttat aaacatacag gaaaaacctc tcaaatgaca
    18301 atagtcactt gcagaggttt aaaaatcatt tgaagaaaga tctatattca aggtttttac
    18361 cattagactg gataatttgt ttataatatt caaaactatc ttttttcaat ctcctaaagc
    18421 ttccgctccc atcattttgt ctatccacat aaataaagcc atatcgcttc ctcatttccc
    18481 ctgttcctgc cgatacgata tcgattggtc cccaccataa gtaaccaatg atttggcaac
    18541 catcttttat tgcctcagca atttcctgaa gatgtatttt tagatactca caacgttttg
    18601 gatcgtggat tcgattcaat tcatctggtg cttcatctag tccaatgcca ttttcaacta
    18661 tgaataatgg taattcataa cggtctgtca attcattcat gacataacga aaccctagag
    18721 gatcaattgg ccaactccaa ggttcgggag atttttcctt caaaaaggga ttatcgaaac
    18781 catttgctcc gccagtatct acgcgcattt ctgcttcttt tttatagaca gcgctacgat
    18841 aataactaaa tgcaatataa tctgccgtgt tagcttgcaa gcactgttcg tctccatcta
    18901 acattactgg tgcacagtct tcttccttcc atgtcctgta aatataagct ggataatgcc
    18961 ctcggcacat gacatcggag aaaaaccatg aattgcgttt aaattgcagt gaaccaaata
    19021 cattctctgg atcacagtca tatgcataag tcgctaatgc agaaaagcta cacataatac
    19081 ccatcttagc atttggatcg attttcttta ataattgagt agcacgggcg ttcgctagaa
    19141 acatatgatg acaggcttga taaatcactt tttttgttag tgtagcaatg ggttcaagtt
    19201 tatctctagc agggaatgta accaatccag cagctgcata aggcatacga aaagcattat
    19261 tgatctcatt aaaggtcatc caatatttca ctttcccttg gtagcgttca aagacggtct
    19321 gcgcataaaa ttcaaaaaag tcgattaatt ttcgattttg ccacccccca tactctccta
    19381 ccaatgctaa gggggtttca taatgactaa gagtaattac tggctcgata tcatatcgat
    19441 ttaatgtatt gattagtctg tcataaaata cgagaccagc ctcatttggc ttctgttcgt
    19501 ctcctcttgg aaatattcgt gcccatgaga tgcttgttcg aaaagctttt aaacctaact
    19561 ccttcagtaa acgaatatcc tcttcaaatc gatgataaaa atcaatcgct tcatgagaaa
    19621 gataatggag tgattcgtct aaagcaatct cccaaatctt tttttcttca ttccactgaa
    19681 tactaggatg ctggttcatg atcccgataa gcgtatctgt cacattaggt aatttgccgt
    19741 cttccaacca tgcaccttca cattgattag cagctactgc tccgccccat aaaaaatttt
    19801 ctggaaatat ttcctgatcc ataactatct ctcctcgcta tcaacaattt cttacttgaa
    19861 gtatagctcg attcatcaaa acaagatatt ctaactattt cggcagaaga gaagaaaaaa
    19921 attgaatgct actcttcatt tttaatattt atatataaca aatacataag acgatccccg
    19981 aaagaaaaca ctcgttaacc atctaagcaa acgatttatt ttacttccaa cacaaatgct
    20041 tcataaggtc gtaaatcaac tttgccgtct ggtaactgtt tcagaggata atttgacaca
    20101 ataaccctta aagcttcacg tggaatagtg aacgtctgct tttcactgga catattacaa
    20161 ataacgagcc attgctggtc ttcatatatg cgagaatagg caaagacatg ctcaggcgtc
    20221 ttttccacta attcatagtc tccccagacg atgatgtcat gttttctacg caaatcgatc
    20281 aatttcttgt acatataaaa aacagaatct tgatctgcta acgcttcttc tgcattgatt
    20341 tctttataat ttggattgac tcgaagccat ggaattccag tagtaaaccc agcatgttca
    20401 cgggcgttcc attgcatagg cgtcctagca ttgtctcttc ctttcgcctt gattgattcc
    20461 aagatttcct cttttgtaaa tccagaagaa atcctttcgt tatacatatt gattgtctca
    20521 atatcttttg cttccgcgat atcatcgatt gggcaattcg tcatgcctaa ctcttctcct
    20581 tgatagatat aaggggtgcc tttcatcata tggagcaaga tagcaaacat cttggcactt
    20641 tcttttcgat acacatcgtc atttccccaa cgagaaacaa ttcgcggaag atcgtgatta
    20701 ttccaaaata aactattcca tccttcatca cctaaagatg tttgccattt ggataatact
    20761 tttttcagtt cagagatact caagggtttc aaatcccatt tgtctttgcc ttcttgctga
    20821 tctaatccta tgtgttcgaa ttggaagacc atggataatt catttctttt aggattggaa
    20881 taaagtttag cgatttcggt agttgctccc catgtttcgc caacagtcat cagatttttg
    20941 tcaccaaaag ttgcttgatt catttcctga agatactcat gcagtttagg tccattactt
    21001 gtgatttctt tatccggttc tttgccaatc atatcgatca cgtccatacg aaagccccca
    21061 atacctttat cgatccagaa attcatcatt tgataaatct cttggcgaac ctgtttattt
    21121 ttccaattca aatcaggttg acgcttgcta tacaagtgca gaaaatattg ccccgttttt
    21181 tcatccaact gccaagcaga accactgaat attgagcgca atctattagg ctcttttcca
    21241 ttgaccggat cacgccaaat ataatagtcc cgataaggat tttctttatt tttacgggcc
    21301 tcaatgaacc acggatgttc atcagaagta tgattcacca ccagatccat gatgatacgg
    21361 atatttcttt tctttgcttc ttcaatcagt tcttccattt cttccatcgt accgaattct
    21421 gccatgattg cttgataatt actgatatca tacccattgt catcattcgg agactcgtat
    21481 actggagaaa gccaaattgc atcaatacct aaatacgcca gataatccaa cctggaaata
    21541 atcccccgca aatctccaat accatcttga tttgaatctt gaaaacttct tggatagatt
    21601 tgataaacaa ctgaatggtg ccaccatttt tgttccatga ttttcctcct ataaatggat
    21661 gtttctattt tttgcttaat catttatttt agtctctctc actaaaaatt gtaacgcttt
    21721 caaatagcta ttgcaccttg ttatcggtaa cagaataaaa caaaaaaaca gctgaggaaa
    21781 ttccctcaac tgttttttga aaggattgat ttatgcaggg aagaatgctg atttcacaac
    21841 ttctttataa acggtgttca ctcagctgtc ccttggtatt agctcaaaaa acggcaaaat
    21901 ataagaaact attttttcgt ttttttctct aatactcagg ccagaacgct gatttcacaa
    21961 cctctttatt tcacattcaa tatccacatg aatcccacaa atacaaagaa cagaccatac
    22021 atgattggag atacttcttt tcctctttta gctgctaaca tggtaattgg atagaagata
    22081 aaacctaatg caatcccatc tgaaatacta taagtcaaag gcatgccgat caagatcaag
    22141 aatgaaggga tcgcaatctc taagtttccc cattcgattt gtcgcaaaga ttgtgccatc
    22201 aatactccta ccataatcaa tgctggtgca gttacttgtg aagtaacaac tgatagtaat
    22261 ggagaaaaga acaagcctaa caagaagaaa atccctgtag taatagctgt gatacctgaa
    22321 cgtcctccaa cggcgatacc agctgatgat tctacaaatg caccaacagg agaagtacca
    22381 aataaggaac ccgcaagcat agctgttgaa tcagaagcta aagctttccc tacacgaggc
    22441 atcttgttgt ctttcatgaa acctgcttgg tttgctaatc caactaaagt tcctgctgta
    22501 tcaaagaaag tcacaagcaa gaatgtcagc actacgaccc acatttgaag agaattgatg
    22561 tcccctacat gttttaatgc aactaaaaat gttggtttca aacttggtgc tgcggcaaca
    22621 atttcagaag gcattggaat caaacctgtg accagaccta aaatcgttgt agctgccatc
    22681 ccgatgaaga tgccacctgg tacacgccga acaagcaaga tagcagttac gactaatcca
    22741 aaaatcgtca accaagtaga accaacgttg agtgatccta atcctactaa tgtagattca
    22801 ttcgaaacga tgatcccacc ttcgctcaga ccaaggaaag cgataaatag accgatacca
    22861 ccagaaatcg catatttcaa atctgctggg atcgcatcaa tgatcatttc ccgcagcttg
    22921 aagattgtga tcaaaataaa aatcaaagaa gcaacgaata cgccggctaa tgctgtttcc
    22981 catggaatcc ccattcctaa acaaacagaa taagcaaaaa aagcattgat cccaagagca
    23041 ggtgccgttc caattggata acgggctaat acccccatta aaacacagcc gatcgcactg
    23101 gctaatgcag tcgcagtaaa cactgctcct tcatccattc cagaagcacc taatacagtt
    23161 ggatttacaa ataagatata agccattgag ataaatgttg taaatcctgc cagtacctca
    23221 cgttttaccg tggtgttcaa tttttctatt tcgaagtagg aaagtatttt ttccttcatc
    23281 ttccatcccc ctaaatccga acattataaa taaagtccgt taaaatttcg atttattaat
    23341 aaaacaacgt gttttatcat atactaaaac aaaacataag taaacaaaaa attcggattt
    23401 taagcaaaat ccgaacgttc catctgatta aatcccttta tctcaaagtt catgaacatt
    23461 cactttggat tacaagaatt tctttattgt gttaataaag tttcttcatt ctagtctcaa
    23521 aaatcaatag tgtgatttga ttttaccatt tcgtttctgt tcttctttga tgaattcttt
    23581 tgtatcgaca ggattcagat tatttagtcc gccaaaacct gtatcgacca tttttcccca
    23641 gccttttgtc ttcttttttc ctgatttgag attctttgct cgaagcttct cactatgttt
    23701 tgctcgccga tacttcaaat aaccttctaa tcttcccatt agatccactg tttttcctcc
    23761 tttttattca atcagacgac tcagcttttc ctataacaaa ataattgcct tcagggtcag
    23821 aaaaattgaa agtcaagatg ccattgttat ctgtgatttc tcctggttct tccatgttct
    23881 cgtagatagt ttcaaattca tccgtaaaaa atactaaaga aggcggtggt cctaatacct
    23941 ctggtgaaaa cttctgcaca aatacttttg ggaatacgcc taattctaat tcttttgaga
    24001 tgctgagtac aatactctta aattcttctg gcaattcgat tgtttcagaa atttctgcac
    24061 caattttttc tacaaagaat cggcaaatca tctctacgtc attgacataa agcatcacac
    24121 gtgttttttg tatcatcatt tgacccttct ttctatttga ttttctttcc tgtctataaa
    24181 ctgattccat tatatcacga aagcgcaaac accttgtgtt tgatactatt caagaaaaat
    24241 aaagcaatag tttctaggat tgctttcctc cacaaacttt ttttacaaaa aaaaaacggc
    24301 aatgaatgcc gctcttctta tttcgattct tttataattc tgcttctgat tcatctttgg
    24361 ttagtgtgtg tctaacacta gttacgccaa atgtttgatc gattttttcc ttcaacgctt
    24421 cgattgcctg tgcgccacgt gccgcttgtt cagcattgat ggattcaatg accagcaccg
    24481 ctgcttgtgt ttcttccgtc agcatcgttc tttgggcttc acgccagttg agacagcgac
    24541 agatcgctcc ttgttcatca tagtagatga tttcttccgg aagtgccgga gcatcggaat
    24601 ctgcacctaa aggcaggaat gattcgccac cttgggcttt tcctaaatgc agatttcctg
    24661 cgatcttttc gatatcttct ccgccgcaag gtacgccaaa agacaaggaa atactattat
    24721 agatatctac aagcggattg attgggaaaa attcccgttc ttgatttgca cgtttcagta
    24781 aagcttcaat agaagaacgt gcaccttttt tagtcttgaa tgtggtaaat gcctgacgcc
    24841 actgagcaat gacttcgttt tggctgaatg tttcttctgt aaggtaattt cttgcttctt
    24901 ttgctgcctc attcaataat tgatgatacg tttcttcact ttcttttgtt acgtggttat
    24961 cgataccatt tacggataag atttcgatgg ttgcttcagg aaatacttcc cagaaattgg
    25021 tatccactat tacttttttc atctgttcac acctcttgat tagaataatt gcttgatgaa
    25081 tagatagtct gtttggggat catctcccat ataaaaaaca tgttcgctgt atttgataaa
    25141 tcctttttta cgataaaaag cgagcgcaac atcgttgtgt tcccaaacac cgagccaaat
    25201 ggctgttttt tgcttttctt ttgctttttc aatagctgta tctagtaaaa atgtaccgat
    25261 accttgatgt ttaaattttt ttcgtacata gattcgctcg atttctaaaa aatgatcagc
    25321 catttcctca gattgactcg ttcctgtatt tagtttcagg taacccgcag gctcttgatc
    25381 gatactcact aggaaaaaat cggtttcttt atcttttagc tcttctgtaa gtttttctag
    25441 tgcataagct tgtgtcaagt aatcagctaa atgctccggt gaattatctt ttccaaatgt
    25501 atctgtaaat gtttcgatac taatttgttg tagtaagtct acatctgaag gcgtcatttt
    25561 tcgaatttcg tatttattca cggcttcttc ctttcaatag atccttttat tgcctttttt
    25621 gacaaaattc caatcttcgc taacattctt gcatacttta tttaaaagat attccaattg
    25681 ttttgcttct tcgtcagaaa gaccatttaa tgcgactgca ttggagtagt tgttttctcg
    25741 aatgatgaaa ggatagattt ctgctccttt tttagtcgga tagagtttct tgattttttt
    25801 attttcttta tcttccaaac gttcgatcat cccattggat tctaatttct tgattgctcg
    25861 ggcagtcgtt gtacgatcta ctttgatcat ttctgctagt ttttcttgga tgattccagg
    25921 attttcgcaa atacgtacta aataaagata ctgtcctctt gttaaatcga cttctttgaa
    25981 ttcgatattg ctaatagaat ctagcgctcg ggcaatcatt ccgatgtctc gaaggatttc
    26041 agccatttca tcccaccttt attttgtttg taagactagt tcacctcttt ttgttgcaaa
    26101 tgcaactatt ttcaataaaa aaatcaagtt cttttttaat aactagtaaa gtatattctt
    26161 ttcttctttt acgaaaaagc tcaatcacaa tagatctggt ttttgttttc taaaataagt
    26221 aactgtttct tcgttgttcg cttttctgat agctttggca actcttctat actaaaaaat
    26281 cccatgttgg aagtttcatt attctcaata aacttggcct cttcaccgtg gatcgcacaa
    26341 gcaaaaacaa gtttataata ctgaaaaagc tgtggaatat ctttttgttt attcgtatca
    26401 aaaactgctc gcaactgagt agtttctact gttaatcctg tttcttcata gacttcttta
    26461 cgcacatttt cttcaggaga caatcctatt tcagcaaatc caccaggaag tgaccattct
    26521 ttcgtccgtt tatcttcgat tagtaggacc ttgccatctt ttttgatata ggcacgaaca
    26581 tctacttttg gtgttggatg tccttctcca atcgttgtga gcgcttttat ttcttcgaaa
    26641 gtttcttttc ctatttcact gatcagttcc aatgaaatcg tccggatctc ctcaaaacgc
    26701 tctttatcaa atacatcttt tccataatat agaccagctt gagaaagtgc gagtagtctt
    26761 ttgtattgag ccaacttctt gttcattctc ttcccttctt ttttagcaaa tgtatgacta
    26821 tgtaatcaag tatagaagac tggtttaatt acggcaaata aatataatga aatctgcaat
    26881 aaataaaaga atgttaaaaa actcgagaac aaaagacatt cgacttctct ggggctgtga
    26941 ataaatggcg ttcaaaagct tgcatctgcg gtaataagcc ccaccaaacg aaaaatatga
    27001 cgagctattc ttgtctggct gttcttatta attgaggcag gacagctttt tcacaacctc
    27061 cccactaaac ggtgttctcc taccaactcc tcggtcacaa gtcacaatat tcatcaaaca
    27121 tgagaagctg tttttttgaa attgctcctt gtgactcgga gctgaacgac aggctcacga
    27181 cctctcggta aacagcgttc aaaagccaat ttttcgatat taagccattt ctttgttact
    27241 tattgtcatt gatttctttt tctaatttat ccaatagtgg agtgtaatcc acttgagtat
    27301 agtctgttac atcaaattca tatagtgttc ctaactggaa tttgtcatat tttcggtcgg
    27361 cgatgatctt tttgaatgct tctttcgtaa tatgattggt ccctttcgtt cgatcttcca
    27421 atacgtcacc atagtgataa tgatccatga tatagctcaa atgacgatct gattcacgat
    27481 cacgtttgta ccgacgttgc cataacactt caaagtctgc tgttaggcga atcgtgatca
    27541 cctgatatcc gtatctatct gctaattctt ggagtcgtgc tttttgctta tcactaaaag
    27601 gatactcgga cacaatcact ttctttcctg cttccatata caaatcaagt gctttgtaat
    27661 agaaaggata aactttcgtc ctttcttgtt ccgcccgttc ttccaaattg ttaaacccga
    27721 tggattctgc atagtattcc ttgatttcat cgagcgtgat ggcatacata tctggaaatc
    27781 gttcccgtag tttattcata agatatgttt ttcctgtacc aggactacca gccagaagaa
    27841 tgaaatactt tttcatctca tttatttcct aagttaattg gactttccaa gttcataaac
    27901 agtccaatct tatgcttgac aatttctttt actgcttcat ttgctggaat cacattatgg
    27961 cgaagagact tgttgatttc tgtttcacta aaggtttctt tagctacttt cgtccagttg
    28021 actaaaagct ccgttccaac attgaatttg cggatcccgt tattgatcaa ttcaggataa
    28081 tcttcttctt tcacacctgt tcctccatgg ataactaggg ggacatcaac tactgcatcg
    28141 atttctttca gcaagggaat gtttacttct gtttttgatt tgaattgtcc atgattcgta
    28201 ccgatagcaa tagctaatgc atccacgcct gtagcttcta caaatttcac tgcgtcttcc
    28261 ggtcttgtat ataccttgtc attttcagcg acatggattc cttcttctgt accgccgatt
    28321 gttccaagtt ctccttctac ggaaacaccg tgcgcatggg catattctac tactgctttt
    28381 gtcttcagga tattttcttt gaaaggcaga tgggacccat catacatgac agaagtatat
    28441 ccgctgtcga ttgcttcttt gatatcatca aagtctcggg cgtggtctaa gtgcaagact
    28501 gcatcaacca tttcttcttc tgcaatagct gtcattacat tgactaacac atgcatgccg
    28561 atatatttag cggtatcgac acttgtttga ataatgatcg gcgaacccat ttcttttgct
    28621 gcgcctagca tagcaggaag catttctagg ttatgcgtat taaaagctcc aactgtaaaa
    28681 ttcaactctt ctgctgtttt tgttacttct ttcaatgttg tatacatctt ttattgccac
    28741 cttatctttt tatttgtttc ctttgatcgt tacttgtttt gcaatttgtc gcatctcatc
    28801 cattttgccc atgtagtaat cgatcccttg atcatcacca gcacgagcag catctgcacg
    28861 cttctggtta tagaggctca tcagtttttt atacccatct ccgatcgtct gtttattttc
    28921 caaactcttt tctaaatatt ggatggcttg atcgttcaca tacaattcag aaaatttaag
    28981 tccaacttgt tcataaagtt tcgctaattt atgcttatct tgggtagtgg aaatctcttg
    29041 tttcaattgt gtaatctctg attgaatctt ttcggtcgct tctggcgata gggctttttc
    29101 ttcttttgcc agaacttttt tcggttcttt ttcttttttc ttaaaaaatc caaacatcat
    29161 tttccctact ttcaattaaa atgctttcaa aactgggctt aagatccagg catataagag
    29221 tgctgcggct actgtatcta catcggttgc tgtagcattg ttgaatccca tcgaattaaa
    29281 gatcgttact agcaaagcgg gcaataatgt aataaagaaa ccatgagcaa taccgccaat
    29341 gatcgctcct cggcgtccgc caactgcgtt gccaaatatc cctgctgtcc ctccggcaaa
    29401 gaaattggtt agcataccag gtaaaatcat cgctaagccg aacatgggca aagtaaacat
    29461 ggcaataatc gtaccaatcg ttgtcgtaat gaaacctaaa atgacagcat ttgggctata
    29521 aggaaagaaa actggacaat ctaaggcagg gatcgcatca ggaactaatt tcatagctat
    29581 ccctcgaaat gccggcacga tttcacctaa cagtagacgt acaccagcta gcaatacata
    29641 cacacccact acgaattgga tggcttgtaa aaaggcaaac atgatatagt tttgatctcc
    29701 tgataattca gaagcgaatg gttcgcctgc aaataaaaca gtgatgatat atagcgggac
    29761 catcacgacc atgacagaca aataagtatc ttgcagaaat tcgaaatgtg tcggcaactt
    29821 gatatcttcg atcgactttt tattttcgcc tttctcacca aaaagcttag caacaccggc
    29881 ttcaaataag tacccgatcg tacaaaaatg acctaaagca atatcatttg atccagtgat
    29941 tttacgaatg atcggttgcg ctactgccgg catagcaatg gcgaaacaag ctccgataaa
    30001 accaccgacc aagatcaata cgatccctcg cagaccggca aaatagccaa agactgtcgt
    30061 catcgttgcc atccataaaa ttgcttgacc agttaaaaaa atgtacttcc attttgtgaa
    30121 acgagccaaa ataatattga atacaaaaat ggctaagaac gtcagtgcaa tatctctacc
    30181 taaacccaaa tcattcattg cttgaccatt gatagattca atagaaggga tgatcccttg
    30241 catatgaaat ccttcagtaa agattttccc gaaataagtt aaacttccaa cgatgatgct
    30301 cgaaccggca ctcaacactt gaaatcctag gagtgtcttt aacgtgccag agatgacttg
    30361 tcctgctgat ttcttctgca gtagtagacc caacatagca atcaatgcaa tggtgatcga
    30421 tgcctgagtc aaaatatttt cgatgataaa attgataacg cccat
//
LOCUS       DACKAC010000030        30274 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.030, whole genome shotgun sequence.
ACCESSION   DACKAC010000030 DACKAC010000000
VERSION     DACKAC010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.030"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..2282
                     /gene="mprF"
                     /locus_tag="I0B46_001400"
     CDS             <1..2282
                     /gene="mprF"
                     /locus_tag="I0B46_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAR2083710.1"
                     /translation="LAAWVTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKV
                     ALFMLTGLSVWSFLTFIDVFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFF
                     EFYPKGLLGLLLASFGQWTGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVP
                     GGMGTFDVLMILGMSQLSVRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRF
                     LDNIPRLFFQKVAHIVLVSAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQT
                     VNLLVGFLLLGLARALSVKVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAV
                     WLSRKEFYRTKFVYSWGAIIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPS
                     DDVWFSGLIGLGISLLALVALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLR
                     LSGYSYFYYQEDDQDQVVFGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQ
                     LAFYKISESYVVMLHELGFHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTW
                     YDQLPEELHQAVNEVSQKWLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITA
                     QPMTKKQAGYDLLRVLPKESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDF
                     SFFQEKVMHIVYNYGNFFYSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSL
                     IGKEKGNVPSLAEEVLTEL"
     gene            2436..3287
                     /locus_tag="I0B46_001401"
     CDS             2436..3287
                     /locus_tag="I0B46_001401"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAR2083711.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            3532..4470
                     /locus_tag="I0B46_001402"
     CDS             3532..4470
                     /locus_tag="I0B46_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAR2083712.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            4493..5173
                     /locus_tag="I0B46_001403"
     CDS             4493..5173
                     /locus_tag="I0B46_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAR2083713.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            5146..5970
                     /locus_tag="I0B46_001404"
     CDS             5146..5970
                     /locus_tag="I0B46_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAR2083714.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            6137..6955
                     /gene="purR"
                     /locus_tag="I0B46_001405"
     CDS             6137..6955
                     /gene="purR"
                     /locus_tag="I0B46_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAR2083715.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            7458..8831
                     /gene="glmU"
                     /locus_tag="I0B46_001406"
     CDS             7458..8831
                     /gene="glmU"
                     /locus_tag="I0B46_001406"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAR2083716.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            8925..9896
                     /locus_tag="I0B46_001407"
     CDS             8925..9896
                     /locus_tag="I0B46_001407"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAR2083717.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      9902..10004
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            10070..10639
                     /gene="thiT"
                     /locus_tag="I0B46_001408"
     CDS             10070..10639
                     /gene="thiT"
                     /locus_tag="I0B46_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAR2083718.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNSWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(10720..11493)
                     /locus_tag="I0B46_001409"
     CDS             complement(10720..11493)
                     /locus_tag="I0B46_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083719.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            11759..13459
                     /locus_tag="I0B46_001410"
     CDS             11759..13459
                     /locus_tag="I0B46_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAR2083720.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            13429..13647
                     /locus_tag="I0B46_001411"
     CDS             13429..13647
                     /locus_tag="I0B46_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083721.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            13667..14557
                     /locus_tag="I0B46_001412"
     CDS             13667..14557
                     /locus_tag="I0B46_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAR2083722.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            14575..14859
                     /locus_tag="I0B46_001413"
     CDS             14575..14859
                     /locus_tag="I0B46_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083723.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            15346..15960
                     /gene="gmk"
                     /locus_tag="I0B46_001414"
     CDS             15346..15960
                     /gene="gmk"
                     /locus_tag="I0B46_001414"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAR2083724.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            15965..16270
                     /locus_tag="I0B46_001415"
     CDS             15965..16270
                     /locus_tag="I0B46_001415"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAR2083725.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            16523..18931
                     /gene="priA"
                     /locus_tag="I0B46_001416"
     CDS             16523..18931
                     /gene="priA"
                     /locus_tag="I0B46_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAR2083726.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            18946..19437
                     /gene="def"
                     /locus_tag="I0B46_001417"
     CDS             18946..19437
                     /gene="def"
                     /locus_tag="I0B46_001417"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAR2083727.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEKELEEYMEEH
                     LDD"
     gene            19430..20368
                     /locus_tag="I0B46_001418"
     CDS             19430..20368
                     /locus_tag="I0B46_001418"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAR2083728.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            20368..21726
                     /gene="rsmB"
                     /locus_tag="I0B46_001419"
     CDS             20368..21726
                     /gene="rsmB"
                     /locus_tag="I0B46_001419"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAR2083729.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            21739..22479
                     /locus_tag="I0B46_001420"
     CDS             21739..22479
                     /locus_tag="I0B46_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAR2083730.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            22476..24545
                     /gene="pknB"
                     /locus_tag="I0B46_001421"
     CDS             22476..24545
                     /gene="pknB"
                     /locus_tag="I0B46_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAR2083731.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            24710..25609
                     /gene="rsgA"
                     /locus_tag="I0B46_001422"
     CDS             24710..25609
                     /gene="rsgA"
                     /locus_tag="I0B46_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAR2083732.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            25622..26272
                     /locus_tag="I0B46_001423"
     CDS             25622..26272
                     /locus_tag="I0B46_001423"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAR2083733.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            26273..26911
                     /locus_tag="I0B46_001424"
     CDS             26273..26911
                     /locus_tag="I0B46_001424"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAR2083734.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            27066..27920
                     /gene="mreC"
                     /locus_tag="I0B46_001425"
     CDS             27066..27920
                     /gene="mreC"
                     /locus_tag="I0B46_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAR2083735.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            27924..28433
                     /gene="mreD"
                     /locus_tag="I0B46_001426"
     CDS             27924..28433
                     /gene="mreD"
                     /locus_tag="I0B46_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAR2083736.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            28688..30226
                     /locus_tag="I0B46_001427"
     CDS             28688..30226
                     /locus_tag="I0B46_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083737.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTVPESSATEE
                     SKTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTPEQPKPVTPAPAP
                     SGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTGRDIGGWTVPQES
                     AGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESVKVGSVQWFAPDF
                     AVSM"
BASE COUNT         9979 a         5400 c         6548 g         8347 t
ORIGIN      
        1 ttcttgctgc gtgggtaaca aacacgatca acaatctcgc cggttttggt ggtgtggtag
       61 gagcaagtct gcgggctagt ttttatggaa agcagacaaa tagaaaaatg gttttagcca
      121 ctgtatcaaa agtcgcacta tttatgctga ctggtttgtc tgtatggtcg tttcttacat
      181 ttattgatgt cttttttatt cagtcagaaa gtatttttcg aagctattgg atctggcttt
      241 tcggcggtag ttttttagca ccagtcattt tcttttttgc ttatctaaag agaaagacat
      301 tgttcttcga gttttatcca aaaggcttgc ttggtctgct actggcatcc tttggacaat
      361 ggacgggagc ccttgcggta tttttgatca tcggcaagtt gatgagaata gatatttcga
      421 tgctttcgat ctatcctatg ttcatgattg ctacattgat aggaatgctg acgatggtcc
      481 caggtggaat ggggactttt gacgttttga tgatcttagg aatgagtcaa ttgtctgttc
      541 ggcaagatgt agcagtagtt tggctattat attatcggtt gttttattat gttctacctt
      601 ttatgacggg acttctattg ttcatcacac atacaggagt caaaatcaat cgatttttag
      661 ataacattcc gcgactgttt ttccaaaagg ttgcgcatat cgtgctcgta agtgctgtct
      721 attttgcggg gatcatgatg gtacttctat caacaatcac taatctgtct gcaatcagtc
      781 ggctatttca agtattattg ccgttctcat tcaatttttt agatcagaca gtgaatctat
      841 tggttggctt tttattactc ggccttgcta gagcactttc agtaaaagtc aaaaaggcat
      901 ttctgcctac gattgcttta ctatcttttg gtattttaaa tacaatcaca cgtacgttat
      961 cttggcagtt gatattggta tatgtcttga ttctgttagc tgtgtggtta tccagaaaag
     1021 aattttatcg aacaaaattc gtttattctt ggggagctat aatctttgat gctagcttgt
     1081 tcggcttttt gtttatcgtt tacgcaattg caggctatca cagcggacgc tttttagtcg
     1141 gtcgaataac aggaaatcat tttattttgt ttccttcaga tgatgtgtgg ttctcagggc
     1201 ttattggttt gggcatctca ttgctagctt tagtggcgct ttatcaatat ttagctgaag
     1261 atactacgaa gcttggcagt gagtacgaaa aagaccgttt tgaccgttta ttggaaacat
     1321 acggaggcac agctacaagc catcagcttc gcttgtcagg ttacagttat ttttattacc
     1381 aagaagacga ccaagatcaa gtcgtttttg gttatcagat caaaggaaac aaatgctttg
     1441 ttttaggaga tccagttggc aatcctaaaa tgatgcgggc agcaacgaat cagtttatga
     1501 aacaagcaga ttgtttaggc tatcagcttg cgttttataa gatatcagaa agctacgtag
     1561 tgatgcttca tgaattgggt tttcacttca cgaaagtagg tgaagcaggg attgttgatc
     1621 tagcaacaga agacttgtct cggctgagtc agcaacttga atatcaacgt ttgatgaaag
     1681 aaggatacca ttttacttgg tacgatcaac tgccggaaga attacatcaa gctgtaaatg
     1741 aagtttcaca aaaatggctt caaggtgcac gagaaaaata cttctctgtg ggacgatttg
     1801 ataaatcata tgtattatct agcggtgtcg gtattgtccg taaagatcaa aaagtcgttg
     1861 gcttcatcac cgctcagccg atgacgaaga agcaggcggg atatgatttg ttgcgggtct
     1921 tacctaaaga atctgaggaa ttagctgact atctgttagc caatctgttt attgtatacg
     1981 agaaaatggg ctactcggaa gccaatctag gcttagctcc attagctaat gttggagaaa
     2041 cagatttttc tttctttcaa gaaaaagtca tgcatatcgt atataattat ggtaattttt
     2101 tctattcctt ccagtctgct tatgaaaaga aattaaaata tgtcgatcat tgggaaggac
     2161 gttattttgc ttataggaaa ggaagtaatt tcggatttgc taccatgcag ctactcagtc
     2221 tgataggaaa agaaaaagga aatgttccga gtttggcaga agaagtgctc acagaattat
     2281 aagaatcact agaacttgtt tggattatga agagaaaatt ctgtgaaagc tgttgaaacg
     2341 atgacaaaat caaccactag gcagacataa gctatacttt tatttttccc tatgatagaa
     2401 tacttgcgac ttagactttt cgaatgggtg agaggatgga attgattgaa aaagcgccag
     2461 ccaagataaa tttaggactg gatgtattat atagacggca agatgggtat catgagttgg
     2521 aaatggtgat gtcgagcgta gacttggcag atcatctatt ttttgaagaa ataacagaag
     2581 acaaaatcat tgtagaaaca aataaagcat ttttaccggt agatgaacgg aacaatgttt
     2641 atcaagctgc tttattgcta aaaaataaat acaatatcaa aaccggtgtc aagatctttg
     2701 ttaagaagaa tattccagta gcagctggat taggcggagg aagtacagac tgtgcagctg
     2761 ctctaagagg gatcaaccaa ttatggaatc tgggctgttc gttagaagaa ctggcagaaa
     2821 tcggtttgga agtcggtaca gatgttcctt actgtgtcta cggaagtacg gcatttgtcg
     2881 gtggcaaagg ggaagaaatc caagtattac cttctatgcc acagtgctgg gttgtgttgg
     2941 tcaaaccaag aatgagtgtc tctacacgta cagtgtttag agaagtagat acgcaagcca
     3001 tccaacatca agacatccac ggacttgctg aagccatccg agagcaagat tatcagcaga
     3061 tgatcaaaaa aatgggaaac agtttagaag atataacggg aaagcggcat ccagtcatcc
     3121 atcaaatcaa agagcgtatg atcaagtacg gagcagatgc tgcattgatg agtggaagcg
     3181 gaccaaccgt ttttgccttg tgtcaacatt attcaagagc gcagagagta tataatgggt
     3241 taaaaggttt ttgtgaggaa gtttatttag tacgaaccct gcaataaaat ataagaagta
     3301 aaataggtga tgaaaaactg aggaatccta aaatggtatt cttcagtttt ttattgtagt
     3361 gaaatggcta gatatgagca gcaactcatc cattttacga tttaatcaag aatatacatg
     3421 cttaaatcag aaattgatgg atttctattg actgtgtttt tttattttga tacattagtt
     3481 cacgttaata aatcgtaaaa ttccgatttg ttcattgaaa ggagcatata aatgaaaaaa
     3541 tatcttttga cagctacggt aatgataggt gctttactgt ttgcagcatg tggaaatacg
     3601 aacaaggaag cagacaaaaa agaggatttg acgatcgtta cgacttttta tccgatgtat
     3661 gattttacaa aagagatcgt aggggatgaa ggaaacgtga aacttttgat ccctgctgga
     3721 acggagccgc atgatttcga accaagtgca aaagaaagag cagaaatttc cgatgcagat
     3781 gttttcgtgt ataatagttc agacatggaa ttttttgtgg attcgttaaa ggattcagtg
     3841 gatagcaaac agaccttgat gatcgaagca gctaaaggaa tcgaccgttt agaatcgcaa
     3901 gaagcagacg aacatgaaga atcagaagaa gggcatggtc attcacatga atacgatcca
     3961 catgtttggc tcgatccggt attagctata aaagaagtca gaacgattgc tgaagaatta
     4021 ggcgaaaaat atccagataa aaaggaaact tttacgaaga atgccgatgc ttatatcaaa
     4081 aagcttgaag cactggatca aaaatactcc gatgagctaa aagatgcaac aaaccgcaca
     4141 tttgtcactc agcatgctgc ttttgcttat ttagcgaatc aatacgactt aaaacaagta
     4201 gcgatttcgg gcgtttcacc agatcaggaa cccacaccaa gtcgcttagc tgagctaaaa
     4261 gaatttgtga agaaaaacaa catcaaagtg atttattttg aagaaaatgc ctcttcgaag
     4321 gtagctgaga cattgtcgaa tgaaacagga gtgaagctag aagtgttgaa tcctttagaa
     4381 agcttgacta atgaacaaat caaagcaggc gaaaattata tttctgtcat ggaaaaaaat
     4441 ctgaaagctt taaaagaaag tatcaattaa gttttagaat ggaggttgta aggtgcgtta
     4501 tatcgaaata tctgatttga ccttttacta tgatgatgaa cctgtactag aagatgtctc
     4561 ttatcatgta gatgcgggtg aatttgtcat tttaactgga gaaaatggag cagccaagtc
     4621 aaccttggtg aaagcatcgt taggattact gaaaccttca aaaggaatga taaagattgc
     4681 tgagaaaaat caagaaggaa aaaaattaag tatcggttat attcctcaac aagttgcatc
     4741 tttcaatgca ggttttccta gtacggtgat cgaattagtc cgttcaggaa gatatccaag
     4801 aggccgctgg ttcaagcgtc tctctgaaaa agatcatcaa catgtacagc gtgctttaga
     4861 agctgtaggg atgtgggata tgcgaaatcg gcgaataggt gaattgtctg gcggacaaaa
     4921 gcagcgaatc agtttagcgc gtatttttgc tactgaccct gacttatttg ttcttgatga
     4981 accaacaaca ggtatggatg aagaatccag aaatgaattt tatcgtcttc tccgacatag
     5041 tgcgcatgtc catgggaaat cagttctaat gatcacccat gatcatgaag atatcaaaca
     5101 gtatgcggac agacagatcc gactggtgag aaaggaggat tctcaatggc gttgctttca
     5161 tatgaattca tgagaagagc gtttcttgcc gctatcttca ttgccggaat cgccccgatg
     5221 ctaggtgtgt ttttagtgat cagaagacaa tcgctgatgg ctgatacgct ttctcatgtc
     5281 tctttagcag gggtagcgtt aggattcttt ttgaacctta atcctacact tacgaccatg
     5341 ctagtcgttg tattagcagc tgtgatattg gagtatctgc gaacgatcta ccgaacctat
     5401 tcagaaatct ccattgccat tttaatggct ggtggtttgg cgctagcgtt agtattgatg
     5461 aatttgagcg gagggaattc agcaacaagt atccaatcct atctgtttgg ttcgatcgtg
     5521 accattacat gggagcaagt catcttttta ggaatcttat tcgttgccat cggtatattg
     5581 ttctttttat tcaaacggcc gatgtatgtt ctgacttttg atgaagatac cgcccacgtg
     5641 gatgggctac ccgttcattt gatgtcgatg atgttcaatg tgattactgg tgtggctatt
     5701 gctgtgatga tcccgattgc cggtgcactt ttgatatccg caatcatggt tcttcccgca
     5761 gcaatcggta tgcgattagg caaaagcttc aatgtcgtga ttgctatcag tgtttgtgta
     5821 ggatttctag ggatgctgtc aggactaact agctcgtttt atctagatac acctccagga
     5881 gcaacaatca cattagtctt tattgctttg ttcctgattg taaatatcat caaacgaata
     5941 ttggttgcct tcagaagaaa aaataactaa attataaaga aaagaagcct tataaaaacg
     6001 aaaaattttt tataggcttc tttttatttt aataaatacc gaactatttt cttatttttt
     6061 cataaaatgc tcgaattttt gaagaaaaac ttatataatg taaaacgata aaatttctta
     6121 aaaggagttg gaataggtga aagtacgtcg cagtgaacgt ctagttgaca tgacacagta
     6181 tttattgaat cacccgcatg aattgatttc cttaacaagc tttgccgaac gttatgaatc
     6241 tgctaaatct tctatcagtg aagatttggg aatcatcaag aaaacattta aagaacgagg
     6301 gtatggaacc ttggaaacaa tcccgggtgc tgcaggtggt gtattgttca tccctgaaat
     6361 ttcatatgaa gaggcgaaaa aatatattga atctctagca gaacgtctat cagaacaaga
     6421 ccgtctgctt ccaggaggat atgtttatct ctctgatttg ttaggagaac ctgaactgct
     6481 gcgtcaagtt ggaagaatca ttgcttctaa ataccttggt gaaaaaatcg atgctgtaat
     6541 gactgtagcc acaaaaggcg tacctattgc ccaagctgtg tcctattatc tgaatgcgcc
     6601 attcgttatt gtacgccggg actcaaaaat aacagaagga tcaacagtaa gtgtcaacta
     6661 tgtgtctgga tcttctgaaa gaattgaaaa gatggaactg tctaagagaa gtttgaaacg
     6721 tggttcacgt gtccttgtag tggatgattt catgaaaggc ggcggaacag tcaacggaat
     6781 gaagagcttg attgaagaat tcgaatcaga acttgttggc atcactgtat ttgccgaatc
     6841 aaaattcaac ggtcatcgcg ccatcgatga ttatacgtca ctgctttatg tcaaagatgt
     6901 agatactcaa acgaaaaata ttacggttgt gccaggaaat tatttttcag aataagaggt
     6961 tgtgaaaaag ccgcttagtt tcgagtagta agaaaaattt tgtgaaaatg gctttccaca
     7021 ttttttcata aatttggctt aataccgaga aactggcttt tgaacaccgt ttatctagag
     7081 gttgtgaaat cagcgtcctg tcctgagtat tagctcaata aacggaaaaa ttgctcctca
     7141 tattttgccg tttattgagc taataccgaa ggtcagctga ttgaaaaacg tttaatgaag
     7201 aggttgtaag cctaccgttt aactctaagt cacaagaaac gatttcaaaa aacagctttt
     7261 catgttttat gagtagtgtg acttgtgacc gaggagttgg caggagagca ccggttacca
     7321 aatagatagg cccgttatgg tctatctatt tttttatcca gaaaacgctg ggataaaaga
     7381 aaatcacttg ttttgttgaa aaaatgggta tactgggata aactgaaatc agcaaactat
     7441 atttaggagt gacgcagttg gacgcacgtt atgcaattat tttggcggca gggaaaggta
     7501 cccgcatgaa gtcaaaatta tataaagttt tacatcccgt ttctggtaaa cctatggtag
     7561 aacatatcat taatcgggtc agtgaaacga aaccagatga agtgattacg atcgttggac
     7621 acggagcaga acaagttaaa gcacaactag gagaacgcag taaatatgcg ttacaagcag
     7681 aacaattggg aacaggacat gctgttttgc aagctgcttc tttcttggaa ggaaaaaaag
     7741 gaacaacgtt agttatcagt ggcgatacac cattattgac tactgaaacg ctgaacaatc
     7801 tttttgaata tcaccaagga aaaaatgcaa gtgcaacgat cttgacggca caagcagaaa
     7861 acccaacagg atatggacga atcatccgtg accatattgg gatcgtagaa aaaatcgtgg
     7921 aacaaaaaga tgcaacacca gaagaagctc ttgtacaaga aatcaataca ggaacttact
     7981 gttttgacaa cgaagctttg ttcgatgcgt taagcaaagt tggaacaaac aatgctcaag
     8041 gtgaatacta tctaacagat atcatcgaaa tcttaaaaga agaaggccat acagttgctg
     8101 cttatcaaac ggatgatttt gaagaatcaa tgggagtcaa tgatcggatc gcgttagcta
     8161 aagcaaatga gatcatgaga aagcgaatta accagatgca tatggtcaat ggcgtaagct
     8221 ttgtcgattc agcaacgaca tacatcgatg caggcgtaga aatcggtcca gacacattga
     8281 tcgaagcagg tgtacaaatc caaggaaata cagtcattgg ttctgattgt gtcatcgggt
     8341 cacattcaaa aatcgtagac agtaggattg aggatcacgt cgtcattgaa aactcagtca
     8401 ttgaaagcag ccatgtcaag aaacatgcag atgtgggacc ttatgcacat ttacgaccaa
     8461 aagctgaaat tggcgaaaac gtccatatcg gaaactttgt ggaagtgaaa aatgctcaaa
     8521 tcggcaaagg aactaaagtc ggtcacttga cttatgttgg tgatgctacg ctgggagaag
     8581 aaatcaatgt cggatgtggt gttgtcttcg tcaactatga tggaaagaac aaacatcata
     8641 cgactgtcgg cgaccacagc tttattggtt ctagcacgaa tatcatcgga cctgtcgagg
     8701 tagctaaaaa cagttcgatt gctgccgggt caacaattac agacaatatt ccagaatatg
     8761 cgctggcaat tgcgagagca agacaagtca ataaagaagg atatgcaaaa aaactccctt
     8821 atctaaacta agacttttgc aaaatcactt gatttattta aaaaatatga ctactattta
     8881 ttaggtagaa ttctagagtt gtgaaaaaga aagtggaggt ccccatgtca aaacattact
     8941 ttgacccaag attaaaaatt tttgcgttaa attccaatcg cccattagca gagaaaattg
     9001 cagcagcagt cggcgtcgag ttaggaaaat catctgttac tcaatttagt gatggagaaa
     9061 ttcaagtaaa cattgaagaa agtattcgtg gatcgcatgt ctatgttatt caatcaacga
     9121 gcagcccagt caatgataac ttgatggagt tgctgatcat gattgacgca ctgaaacgag
     9181 caagtgcaaa aacaatcaac gtagtcatgc cttattatgg ctatgcgcgt caagaccgta
     9241 aagcacgtgc acgggaacca atcacagcga aattagtagc aaacatgatt caaaaagctg
     9301 gtgcaacaag aatgctgact ttggaccttc atgcagtcca aatccaaggt ttctttgata
     9361 ttccagtcga tcatttgatg ggtgcgccac ttattgccaa ttatttctta gaaaaaggaa
     9421 tcaaaggcga tgatgttgtc gttgtttcac cagaccatgg tggtgtcaca cgggcacgta
     9481 aattagcgga atttttgaaa tctccgattg cgatcataga caaacgccgt ccaaaagcaa
     9541 atgttgcaga agtaatgaat atcattggac aagtcaaagg caaaacttgt gtactgatcg
     9601 atgatatgat cgatacagca ggtacgatca cattggctgc caatgctttg aaagaagctg
     9661 gtgcaactag tgtgtatgct tcatgtacac atccagtatt atccggccct gctttacaaa
     9721 gaatagaaga ttcagctatc gagcgtctag ttgttacaga ctcgatctat cttccagaag
     9781 atcgtaaaat cgacaaaatc gatgaagtaa gtgttggcgg actaatggga gatgcaatca
     9841 aacgtatcca tgaaaacaaa ccagttagtc cattgtttga aacaaaaaat aaataataaa
     9901 taaactcaca aaggggagtc caattgggct gagattgaat ctatttctaa acccttcgta
     9961 cctgtatcgg ttatgcgagc gtaggaattg tgaataaaca gctgtttgac tattttcgaa
    10021 agctgcttcc tcctttgtga cttttttatt caaaggagga agttttttta tgggaaaaaa
    10081 tagagtttgg gtagaaggaa cgattgttgc agcgttagcg atggtgttgt cgttgattcc
    10141 gatccagatc ggcagcagtt tttctatttc attagggcag attccattga ctctttttgc
    10201 gttaagaaga gggacaaagg cagggatgct ggcaggtttc atttggggca tcctacattt
    10261 tccgttggga caagtttatt atttaagtgt cgttcaagtg ttgattgagt acccgattgc
    10321 ctatactttt gcaggcgcag cagggctcat ggcttcaaat gtccaaaaag gctatgtaga
    10381 cgataatacg aaacaaatta gaaaaagcat cgtgtttggt gcattgattg gcgcactttc
    10441 cagatatttt tggcatttta ttgcaggagt ggttttctgg ggagcgtatg cactatgggg
    10501 gatgaattct tggctctttt ctttcgtaat gaatggtgcc agcgggttga gtacagctat
    10561 tgtaacgatc atcgttttat tagggatcca acaaacgact ccttcgattt ttgtgccaga
    10621 taaacgacag gagctgtaaa taaaaaagaa gcaagaaact tgagacagaa tcatgtccta
    10681 gtttcttgtt tcctttttat tttttgataa gaaatagatc tacttcaatg gcagataaga
    10741 aagatcggca tcttctacca attgccagtg tcgatcggtg acagagtaga ttaaagtagc
    10801 gatgatttcc tcttctcgat accgggtgcc ttttaatttc caaatataat cttcaatcac
    10861 atagattggg atacgatatt taccatcgat caaaccaact tgatcaaaag aaaaaatgat
    10921 cccttcctgc atcaaattac gcatttcttt caataattcc acactgattt tttcaacagg
    10981 tgtttctttt ttgaaaaaag atttttttac attgatctta tctgacaaga tcgaacttaa
    11041 tttactagca aaaatcctat aaaattcatc taaatagcga taataaatcc gattcagctg
    11101 atcattaaga tcgaagtata caaaattatt ctgtagttta taaaaaaacg gcgaatgaag
    11161 atgtgttttc atatgaccaa aatataataa ttctgaaatt tctaaaggtg tcaattcttt
    11221 cagcatcgtt aaatcagtaa agtcgatcca cttcaattca tcgaatccac gcctgttacg
    11281 agaagaggaa aagaaacgct gaatattttc tgtaccacgg attactttca gccctgtatg
    11341 ggattcatat tctccagcag aagcagatgg atcaagcaag agcaagtttt gaggaaaatg
    11401 cacgatactg tttgcaaagt cagtagcagt cagccctttt gttaaaacag cattacttgt
    11461 ggtatcaata tgtacataaa ctgactcagt catcatgttc ctcctcttat ttcctcttaa
    11521 tctcttttat tttacaacat ttgcattaaa aaagcttgtt tcttttattt taaaattcca
    11581 ttacaaaatc acgttttcat tgaaaacact ctcattttca attaattttt gtgatttttg
    11641 tattttttat ttaaaggatg ttttatgaat tgttttctta tggaatctac aaagtcgaat
    11701 acgagactca tgaccttttt atgacgtact ggtatacttg aagaaaaagg agagcgcgat
    11761 ggagataaaa aaagtttact tttcacagaa aatgtcgctg tattcgccta tccagcaagc
    11821 aaaacaaccc cttccatttt ttcaaagtca tcaagaaagt agagacgctt tttggcgttc
    11881 tgcaaaaaag caatgtgctt ttaaagcagt atcgaaaaaa tataccgtct attttactat
    11941 tgctaaaagt agaggagatg ttatttttaa taatctgttg attgaaggtg aaacttttga
    12001 atggaaaaaa ttttttcggt atatggaagc ttgcgctcgt ttttttatta aagaaaactg
    12061 ttgtttttta tttcaatctc ctctatcaga agaatggttg cgtatttttc ataaaaacgg
    12121 ctatcaagga accacacaat taaataaaaa gctggtctat catacagcac ttgttttagg
    12181 tggaggagga gcacacggtg cttatcagat tggtgtgtgg caggcgctga aggaacatga
    12241 tatcaacttt gagattatca caggtacctc ggtaggtgcg ttgaacggcg cattgatttt
    12301 acaaggagat atgaaaaaag ctgtgaattt atggaaaaag ctgtcgacaa gacaagtgct
    12361 tgcgttacca gaaatggctg cagttgaaga ccttcgtgaa cgcttctatc gagaaagacg
    12421 ccaaatgacg aaaacagcac taattgaagg cggggtttct tctgctccat tggaaaagat
    12481 ggtcaaagac aatttggatt tgtccttact caaacataat cccaaaatac gtttatatac
    12541 ggtgtctact aaactgccag aactagcgga agtagtgact gatatccaac agacaaaaac
    12601 agaagaaata ccagattgga tactcgcctc tgcttcattt tatcccgcta tggcttatcg
    12661 aaagatcgga aaaaataaat atgcagacgg tgggtatcgc aacaaaatac ctattgatat
    12721 tgcgattaac aaaggagcaa cagaggcatt tgtcgtagat gtgcaagggc caggcccagc
    12781 gaaaaaaatt cgtatgccag acatttttat ccattggaaa tgtcaaacat tgtggacact
    12841 aggcagcttt ctgctttttg atagccaaag gaaccaatta aatttacaat taggctatct
    12901 ggaaatgaaa aaacgattag gctattacta tgggaactgg tatacatttc attctgttaa
    12961 acctgcaaga acatactggc gaggattttt gtcttactta acaaaagaaa ttcagttaga
    13021 attatctttt ttcaaaagca taaaattttg gcaaaaactg cgtaatttat ataagaacag
    13081 ggtcgtacca gagacatgcg gattggcgat gctagaactt ttagcaaaaa aacatttttt
    13141 actgcccaat gaaatctatc aagtagacat gatgattcaa atgatctgcc aacaagatat
    13201 aaaaagtatg ccggatgatt tattggcaca aattggtcaa ttaagtactg aagaatggcg
    13261 aaggtatcga aaataccagc aaaaaataca aaatgagcgc acgaaaactg tttattttga
    13321 ttatctgcta caaggaaaac gcaaggatcg tttgcagcat gaattgctta agcaaccagt
    13381 tgacacatta ctgatcctat atttatatta tctcaaggag gaacaaccat ggcacaagaa
    13441 ttttcatatg aaatcgtaga agaaatcgct atattgtcag aaaacaacaa ggggtggcgt
    13501 aaagaactga acctagtcag ctggaatggt cgcccgccaa aattcgatct tcgagattgg
    13561 gcgccagacc atgaaaaaat ggggaaaggc ctgacattga cgaacgaaga gtttgaacaa
    13621 ctgcaaaaag caatagaaaa tatgtaatga ggaaggaccc tgtcgaatga aaagtatgac
    13681 tggttttggc aaagccatcc gcgaaacaaa agcgtatcag ttagatattg aaataaaaag
    13741 tgtcaaccag cgttttctag atgttcaaat acgaagtcct aaaatcctga actttgttga
    13801 aaacgatttt cgtcagctta ttaaacagca gttaagccgc ggtcgaatcg aagtatttgt
    13861 gaatctagct tatattggca aaaacgaaaa acagatcgtc atcaactggg aattgattga
    13921 ttcacttatc actcaattga cagacggaat tcaacaacga tacggtagtc aaaatcaagt
    13981 gtcaattagt cggatggcgg aacgcttcgc gctggacgaa aacttcgtaa cggtagaaga
    14041 aaaaccggaa gagagcatgg aagaattggc acagttagct ctagatacat tgaaaagcgc
    14101 acttgattct attgaggcga gtcgagaaaa agaaggtcaa gctttagctt tagtgttgaa
    14161 aaagaatact gaagaattca aagaagtact aaaaaaactc gatagttttg tagaaatcta
    14221 tgaaaaggaa tttcaaataa ggtatcaaaa aaagctggag gaatttcttg gagcgaaagt
    14281 cgatgagcaa cggttgttga cggagctagc catcttgttg gaacgcggag atattcacga
    14341 ggaactggat cgtctggcga tccatgtaca aaaactagat gaacttatcg aagcacaatg
    14401 tcctgtaggt cgggaattag atttcctgat ccaagaaatg aatcgggaaa tcaacacgat
    14461 cggttccaaa tcaaatgcaa tcgaaattaa aaatcaagtc gtccagtcta agacgatctt
    14521 agaaaaaatc agagaacaag tacaaaatat tgaatgacga aagaggtaga gataatgaaa
    14581 tattatgacg taacgttcca cgaattaagt ggaaaaactg tggttaaacg ggatatccct
    14641 tctgaaaaaa acggctttga cgtatggaaa gatgcctgtg cagactataa tgagaacgaa
    14701 ctgtttattt tgatcaacga cggtgcttac gtgacgatga atcggaaatt tattgttcga
    14761 attgacacag aagaggtaga agatccgaca gaaaaagcac gttctcgaaa agatgagatc
    14821 atgggtgtag tgaatacttt gtccaatatg ggcttttagc aaggatgctg aacaaagaaa
    14881 tacaataaat aatcagaatt ggatttcttc gcttagcttc tttgctctag gaatacattt
    14941 aaaggttgtg acaaaattcg tgtcacaatc tttttttatt ttgaataagt gatgttcaaa
    15001 aaccattttc tcggtattaa gccaaattta tgcaaaaatg tgagaaacta ttttcacaaa
    15061 atttttcttc atactcgaaa ctaatcggct tcttcacgac ctcttataca cggcgttcaa
    15121 aagcttgcat ctgcgataat aaccccaacc aaacgaaaaa catgagaagc tgtttatgtt
    15181 tggctgttct tattacttga tgcaggacag cttttttacg acctcttggt acagggtagc
    15241 ttttttacca atttctagct ataattaaag ataaccttga aagttgcaga aaaaaagtgc
    15301 aaaataaacg ataagtacaa aaagactagt tgaaaggacg tcgtcatgtc agagcgtgga
    15361 ttattaatcg tgttgtctgg tccatccggt gtaggaaaag gcacagtacg aaaagcaatt
    15421 tttgagagtg aggataatga ttttcagtat tcgatctcaa tgactacccg taaaatgcgt
    15481 gaaggggagg tcgaaggcgt tgattatttc ttccgaagca aagaagagtt tgaatcaatg
    15541 atcgaagctg gagagatgct ggaatacgca gaatatgtcg ggaattatta tggaactcct
    15601 ttgtcatatg tcaacaagac attggatgaa ggaaaagatg ttttcttgga gattgaagtc
    15661 caaggagcaa aacaagtcaa agaaaaagtg cccgatggtg tattcatctt tttgactccg
    15721 ccagatttag cggaattaaa atcacggatc gtaggccgag gaacagatgc agatgaagta
    15781 atcgaagaac gaatgaaagt tgcaaaagaa gaaatcgaaa tgatggctct ttacgactat
    15841 gctgttgtaa atgatgaagt gccgctagca gttgaacgaa tcaaaaacat tattgccagt
    15901 gaacattttc gagtagatcg cgtaatcggt aaatatatta aaatgttaaa ggagatgtag
    15961 tcttatgatg ttaaaacctt caattgactc actattagat cgtgtaaatt caaaatattc
    16021 tctagtgatt ttagcaagta aacgcgcgca tgaattggat gcaagtgcac agcctactct
    16081 agattctttt gattcagtaa aaagcgtcgg tcaagcttta gaagaaatcg atgcaggaaa
    16141 tgtagtcaat gatccacatc ctgaattaaa acgtgaacgt ttgaagatgg aagaagagga
    16201 acgtcaagca caaaaagagc gtgaacaaca cgaattggaa tcacgtatcc gtgaagagca
    16261 aaaaatgtaa taaagaaaaa tagagcgaac atctaaaaag aaaagcaaac ccagctgttt
    16321 ccttttcaaa cattcgccac ttattgtctg gtagaaaagg ctgtgtctga actttttgac
    16381 acagttcttt tttttcttat ctgctgttaa ttttttgtta ggatttggca gaagataaga
    16441 caaatccatc gatctctagt aaaataggca aggaactttt tgtcgcttga taagaaaaga
    16501 caaaaaaagg aggaaacgaa ccatgacatt aacagctgaa gtcattgtgg acgtacctac
    16561 tatgcagacc gatcagccat tcacttattt ggttccctct gaaatggaaa cagctttgca
    16621 agtcgggatg cgagtagaag tcccttttgg taatggcaat cgtcatgtcc aaggatttgt
    16681 tatggctatc caaaaatcag aagaatcagc taatccatct ttgaaggcag ttattcgttt
    16741 gctagacctt gctccggtgg tcaatgaaga attactttca ttagctgatt atatgaaaaa
    16801 aattacttac gcattcaaaa tcacttgtct acaaacgatg cttccgagtg tgatgaaagc
    16861 agaatatgat aaattgatct atcctttggc agacacacca gaagttcagg tattgtttga
    16921 tcagcgagaa gtgatttcct ggaaggaagc agaagaagaa ggcagccttt ctcaattgat
    16981 tcgctggcgc caagagcaat tagtagatat caaatatgaa gtccatacac gaaataaagt
    17041 aaaaacgatt cgtttggtcc gttctttgtt aactgaaaag caaatcgaag aagaatgggc
    17101 aaaattacgg caaaatgcca agaaacaaaa agaacttctt ttgtgtttat ctgaactgag
    17161 ccaagaagaa ccaattgctt atttcaaaaa taaaggaatc agtactgctg tcttgaatca
    17221 aggaaaagaa aagggctggc tggagtttat agaatcagaa cgttatcgcg acccttataa
    17281 agaccgagta tttgaccaaa cgacggcatt ggaactgaat gcagaacaaa aaaatgctgt
    17341 cgaacaaatc atcacagcag gtcagcagca aaaagatcag gtctttttgc tggagggaat
    17401 cactggcagc gggaaaacag aagtatatct tcaggcaatc gctgatgttt tgtcagaaaa
    17461 taaaacggct attatgttag tgcctgaaat cgcattgact ccgcaaatgg tcgagcgatt
    17521 caaaggacgc ttcggagaat cagttgcggt cttacatagc ggactttccc aaggtgaaaa
    17581 atatgatgaa tggcgcaaga ttgagcggga agaagcgcaa gtagtagtag gcgcacgatc
    17641 tgctattttt gccccgctga aaaatatcgg gctgatcatt atcgatgaag aacatgaatc
    17701 aagttacaaa caagatgaga caccgcgtta tcatgcgaga gatctagcaa tttggcgcag
    17761 taaatatcat cattgcccaa tagttctagg aagcgcgacg ccctctttag aatcaagagc
    17821 acgagctcaa aaaggcgttt atcagttatt gcagttaaac caccgagcca aagcagctgc
    17881 acagcttcca gctattgaga tcgtagatat gcgtgaagag tttcagaatc atcggacctc
    17941 tactttttct gctaatttgc aggaaaaaat acaaaatcga ttagacaaaa aagaacaaac
    18001 tgtcctactg ctcaatcgtc gaggatattc gtcttttgtg atgtgtaggg actgtggatt
    18061 tgttcttcca tgtcctaatt gcgatatctc tcttacactg catatggata cacgttcaat
    18121 gcgctgccat tattgcggac atgaagaacc tattcccaat cgctgtccta attgtggcgg
    18181 caataaaatc cgctactatg gaacaggtac tcaaaaagta gaagaggaac tccgagaact
    18241 ttatccgcaa gctaggattt tgcgtatgga tgtagatacc actcgaagaa aaggggcaca
    18301 cgaacagatt ttacaaaaat tcggtgcaaa agaagcagat atattattag ggacacaaat
    18361 gattgctaaa ggtttggact ttccagaagt gacattagtt ggggtgttga atgctgatac
    18421 ctccctgaat ttaccggatt ttcgttctag tgagcataca ttccaattgc ttacccaagt
    18481 ttcaggacga gcagggcgag cggaaaaagc aggcgaagtc gtgatccaaa cattcaatcc
    18541 tcaacattac gccatagaat tggctaaaaa acaaaattat gagcagtttt atcagcagga
    18601 aatgcatgta cggcatcgtg gaggctatcc tccttattac tttactgtga aaataaccgc
    18661 cagccatcct gaagaacaag tagcggcaaa gaaaatcttt cagattgcca atcagctaaa
    18721 agaagtgtta agccctcaaa gcttgctatt agggccaacg cccagcatga ttttgagagt
    18781 gaaaaaccga tattattatc aactaatcat caaatataaa catgaaccga atcttcctca
    18841 tgttttggat gaaattttga atggaagcca gaaagaacag cgacaaggtc tttttgtggc
    18901 aattgacaac gaaccattaa actttattta agaaagggcc aaattatgcg ctatcctata
    18961 ttgatccacc ctaatgataa attaaaaaga actgctcagc caatcgatgt aatcacggac
    19021 gagacaatcg ctttattaga caatttatat gagacaatga ttgcgaatga cggaataggg
    19081 atcgctgctc ctcaagtagg acaaaacaag agaattgctg tgatcgaagt agatgaaggc
    19141 gagaaatttg aactgattaa cccagaaatc atcgaagcaa aaggtgaaag ccttgacgta
    19201 gaaggttgtc tcagtattcc gcatgtctat ggaacggtca agcgagcaga tgaagtgacc
    19261 gtgcgttatt atgatcgaga cggagaagag atagaagtga cggcttttgg ctatttagcc
    19321 cgtgccttcc agcatgaaat cgatcactta gacggtatct tgttcattga gaaaatgata
    19381 cagcagatcc cggaaaaaga acttgaagaa tatatggagg aacatcttga tgactaagct
    19441 agtatttatg ggcacacctg ctttttctgt gccgattctt gaagggctga tagaatcagg
    19501 ttacgagatc caggcagtgg taacacagcc cgaccgtcca gttgggcgga aacgagtaat
    19561 tacgccgaca ccggtaaaag aagcggctct gaagcacggt atccgtgtgc tgcagccaga
    19621 gaaaatctcc ggttcgccag aaatggaaga aatcattgaa ttggtgcctg atgtgatcgt
    19681 cactgcggca tttggccaat tcttgcctga aaagttattg caagtaccaa aattaggggc
    19741 aatcaatgtc catgcttctt tacttcctaa atatcgggga ggtgcgcctg ttcattatgc
    19801 catcatgaat ggggagaaag aaactggtgt aacgatcatg gaaatgatca aaaaaatgga
    19861 tgcaggcgga atctatgcgc aagaaagcat accaatcact aaacaagatg atgtagggac
    19921 gatgtttgaa aagcttagcc ttttaggaag aaagctattg ctagaaacat tgcccaatat
    19981 tttggatgga caaaaaccag tacctcaaga cgagtcagaa gcaacttttt cgccaaacat
    20041 cacacgggaa caagaagcaa tcgattggca taaaactgca gaagaaattg acaaccaagt
    20101 acgcggaatg cgtccttggc caattgcatt cacgacctat gagggcatac gctggaaatt
    20161 attatcggtt gaagtattgg cagaaactac agacaaagca cctggtacga tcatccggaa
    20221 agacaaaaaa aatctatgga ttgcttgcgg taaacagacc gtcttagcca tcaatgaatt
    20281 acagccagct ggtaaaggaa aacaggccgt acatgatttc cttaacggaa gcggtcagca
    20341 agttcagatt ggtcagcagg tgaaataatg aaaaaaatcc ctaaaaaaat gaaaaaatca
    20401 gtacgatata tggcattact gacattagag cgagtgaaca agggtggcgc ctactcgaat
    20461 cttttgttga atgagatgat caaacaaagc gagctaaatc aaaaagatgt gggtttattt
    20521 acagaattga tctatggtac gattagccat cagcgactat tggaatttta cgttgcacca
    20581 ttgatcgcta aagcaaaaaa agtggatgac tgggtcaaga cgcttcttta tctttctgta
    20641 taccaattag aatttttaga taaagtccct gcacatgcta ttttaaacga ggcggtcgaa
    20701 atcgcaaaag taaaaggaaa tcctgggaca gggaaatttg taaatggtgt cttgaggaat
    20761 tatcagcgtc aaggagcacc agatctgcaa ggcattgctg atcctatcga aagactttct
    20821 gtcaaaatca gcctgcctct atggatgact aaacgactgg tggatcaaat cggatacgaa
    20881 gaaactgaaa agctcggttt atcgctatat cagcctagcc atgctagtgc ccgagtagat
    20941 acacgccgac tttccagaga tcaggccatc actgttttgc aagaagaaga gatcacagct
    21001 gaaaaaagcc aagtcagtcc atacggtata gtagcgaaaa aagggcattt agcttctagc
    21061 tcattgttcc acgatggcgt catgacgatc caagatgaaa gttcgatgct tgtagcgccg
    21121 tctatgcaaa tcgagcctga acatcatgtc ttagatgcct gtgcagcacc cggaggtaag
    21181 acgacgcata ttgcttcttt tttagatccg tcatgcggtg gagaagtggt cgctttagat
    21241 gtacacgaac acaaagtcaa actcattgaa gataatgcag cacgtctagg tgtctcttct
    21301 gctgtctacg ctcaaaagat ggatgctaga gaagtcagta aacagttcga agcagagcag
    21361 tttgatcgga ttttagtaga cgcgccttgt tcaggtcttg ggctgcttcg gcgaaaacca
    21421 gatattcgtt ataaaaaaca gccggatgaa ttagcgaatt taccaaaaat ccaattggaa
    21481 attttagaaa gctgcgcacc aactttaaag tcttccggta ttctgactta tagcacttgt
    21541 acgatcttaa aagaagaaaa ccaagacgtt gtttctgctt tcttggaaag acaccctgat
    21601 tttgagagga ttgatgtttt gaccaatcct atgctacaat catcaatcga agagaaaatg
    21661 ttgactttgt atccgcatca gttttacaca gatggatttt tcatttgttg tttgcgcaaa
    21721 aagtaatgag gtgaaaaaat gcagattgaa tatcaatcag atgtaggaag acgacgtaac
    21781 acaaatcaag actatgccag tgtatttacc agccgttcag gtctcaagct tgctgtatta
    21841 gcagacggga tgggtggtca tcgtgccgga gacattgcca gtcagatggc ggtaacgaat
    21901 ctaggcgaag cttgggaaaa acaagaagct gttgacgatg aaaaaatcgc ccaatggttc
    21961 attcaatcga ttcaagaaga aaatatgctg atttatcagc gtggacaaga acagccggaa
    22021 tataacggta tgggaacaac aatcgtagcg gccgctctgt cagatgactg gttcacgatt
    22081 gccaatgttg gggatagcag agcttatttg attcgtgaag ataaaatcat gcaattgacg
    22141 gaggatcatt ctttagtcaa tgaactagtc aaatcaggag aaatcagcga agaaatggca
    22201 gtgaaccatc caaggaaaaa tattttaaca cgttctgtcg gcatgcctgg aacagtcgaa
    22261 gtagatgttt ctactcatct ttggcaacta aaagatcgac tgctgttatg ttcagatggc
    22321 ttgacgaaca tgctgtctga agaagaaata ggaacgatca tcaatcaaga aggcccattg
    22381 tctcataaag taactgagct gattgatcgt gccaatgaag caggcggagc ggataatatc
    22441 accgttcttc ttatcgaata taaggaggac agtgtatgat cgagttgggc aaaaaattaa
    22501 atggccgtta tcaaatcact ggaaatatcg gtagcggtgg tatggcaaat gtatttttgg
    22561 cgcacgattt aatcttggat cgtgatgtag cagttaaagt cttacgattt gactttcaaa
    22621 atgaccagac tgcgattcga cgatttcaga gagaagcttt agcagcaaca gaactcgttc
    22681 atccaaacat tgttagtgtt tacgatgttg gtgaagaaga caacatgcag tatctggtga
    22741 tggaatacgt caaaggaatg gacttgaaac gatatatcca aacacattat cctgttcctt
    22801 atgaaacagc agtaaatatt atgcagcaga tcctttctgc tatttcttta gctcataacc
    22861 atcaaatcat ccaccgagat ttgaaacccc aaaacgtact gatcgataat gaaggtgttg
    22921 taaaaatcac ggatttcggg attgctatcg ctttatctga aacatcgatc acgcagacga
    22981 atacgatgct aggatctgtt cattatctgt cccctgagca ggccagagga agtatggcaa
    23041 cgaagcagtc agatatttat gctttaggga tcatccttta tgagatgttg acgggtagcg
    23101 taccgtttga tggtgaatct gctgtaacaa ttgctttaaa acattttcaa gatgatcttc
    23161 catctataaa agcacttgat ccaaatgtgc cacaagcact ggaaaacgtg attttacggg
    23221 caacggctaa agaacctgca gaccgttata agtcagccga ggaaatgtct gatgatcttt
    23281 ctacagtatt atcaccagct cgtgcaaacg aagaaaaatg gcagcctcat gtgatggata
    23341 atgaaacaaa agttatcaca ccccttacgg aagatgcacc aatgcctgat tcattcaaat
    23401 ctatgcctct gccaaaagac aagccagaaa acgaaacctt accagaggag gaagaagttc
    23461 tgcctgatcc taaaaagaaa ggacagaaaa aatggtggct gatcctatta gctattcttg
    23521 ctgttttagg aatcggaaca gctgtattct ttgcttctgg cgggcgagga gaagttgttg
    23581 ttcctgatgt ttctgatctt tctgaagctt ctgctagaga aacactcgca aaagcgaaat
    23641 tagaagtagc aacaaaaaca gaagaaattg cagatgacaa aatcgaagaa ggtaatgtag
    23701 tgaagacaga cccggcagcg ggaacgacag tcaagcaaaa acgcgaagtc actctgtata
    23761 ttagtagcgg cacgaaaaaa atcaaattag atgattacag tggtaaaagt tatgaagatg
    23821 ccgaagcaag tttgaaagaa ttagggtttt ctgctgactt gattcaaaaa gaagaggaat
    23881 tcagttcaga ggtagaaagc ggaatgatca tttcgcaatc tccatcagaa gatacagaag
    23941 tcgatcctaa aacagacacg attactttca cagtcagcaa aggaccagaa acagtgactt
    24001 tgagtagtca tgtaggagaa aactacgaaa ctgttcgtaa ttggcttgtt acacaaggat
    24061 tcaatatcaa taatattagt gctacttatg actatagcga cactgtacca tcaggttcga
    24121 tcatcagtca ggatccagcc tcgggtgaag ttgtagcaga agaaacttac atcaattttg
    24181 tcgtaagcca aggaacagag cctaaattga atgatatctc cggctataca aaaagcgaag
    24241 cacaaagtta tttagctagt gtcggtgcag aatatatcgg gcatgagact tccgaatatt
    24301 caaacacggt ggataaagat aaagtgattc gtacagtacc aggggcagga acgacaatta
    24361 ccaaaggaat ggttgtgaac gtaatttact ctaaaggacc agatccagcg tcttctagtt
    24421 ctgaaaaaga ctcttcagac agttctagca gctcaagtag ctccagtagc acaagcaact
    24481 cttcaaaaga agaatcttcc agctccacaa gttcatcagc tagcacctca gaaagttcaa
    24541 aataagtgct gaataaaata tatcagaagg gaatgcgaca ttgttttgtt gcattctctt
    24601 ttttgtgttt ctatctcttt tgatcaattt tcaagccata tactttgaga agtttgcatg
    24661 gaataacacc tactttcctc ttcgtatggt aaaataaaca aaaacggagg tggcacttat
    24721 caaaggacaa atcagaaaag caatcagcgg tttttactat gtatacgcaa acggagagac
    24781 ctatcaaaca agaggaagag gaaatttccg gaatcggaaa atcacgccgc ttgtcggaga
    24841 tcaggtgatc tttgagagtg aaaatcaaac agacggctat cttctggaaa tcttgccgag
    24901 aaaaaatcaa ttagttcgcc caccagtcac aaatgttgat cttggaatca ttgtcacaag
    24961 tatcgtagag cctgcatttt cttataatct acttgaccgc tttctagtta ccttggaata
    25021 cgagtcgatc gaaccgatta tttttttgac aaagaccgac ttgtcagacg acccagaaaa
    25081 aatcgaagag atcaagcaaa cctatactaa aataggctat ccggtgattg tacccaatca
    25141 tacgggtgat acgacagaac tgatccgtta cttcccagaa cggctatcgg tctttatggg
    25201 tcagtctgga gcaggaaaat cgacgctact gaatcaaata tcaccggaac tgaatttagc
    25261 aacaggagag atttctgatg cacttggaag aggacggcat acaacacgtc atgtggaact
    25321 gattcctatc tatggcgggt tagtagcaga tacaccagga tttagctcga ttgacttttt
    25381 gacaattgaa gcaggagaac tgccgagaca atttcctgaa tttgtagaag cttctgtcca
    25441 ttgcaaattt agagagtgca tgcatgctaa agaacctggc tgcgaagtaa aacatagagt
    25501 agaaacaggt ggtatcgcac agacacgtta cgacaattat ttgcaattct tacaagaaat
    25561 cgaaaaaaga aaaccaatgt atgcaaaaaa agaaggtaaa cgaaaatagg aggaaacaag
    25621 aatgaagata gcaccatcca ttttgagtgc agattttgcc aatttacaaa gagacattga
    25681 gttagtggaa aaaggaggcg cagattacat ccacgtagat gtaatggatg gccaatttgt
    25741 cccaaatatc acattagggc caaatattgt tcaggcaatc cgtcctgtta ctaaattacc
    25801 gttagacgtc catctgatga tcgttgatcc agaaaactac attccagcat ttgcaaaagc
    25861 tggagcagat atcattactg tccacgtcga agcaactcct catattcatc gggcacttca
    25921 aatgacaaaa agcttaggag ttaaagctgg agtagttatc aatccaggaa caccagtctc
    25981 catgatcaaa catgttttgc ctttggctga tcaagtacta gtcatgactg tcaatcctgg
    26041 gttcggcgga caaagcttta ttgaagaaac agtagaaaaa attacggaac tcgctgatct
    26101 acgtagagag aacaattgga cttatgcaat cgaagtagat ggcggaatcg ttccagaaac
    26161 agcaaaaatc tgtaaagatg caggggcgga tgtcttcgtt gcagggtcat atatctatgg
    26221 agccgaaaat ccagtcggac aaatcgaacg attaaaagag gcattagact aaatgaaaat
    26281 tttattagct gctggaggta atccgcaaaa ctggccgcag tctctgctgt ccaaatacga
    26341 tgcctatgtc gggatagacc gtggtgcgct gtacctgctt caagcaggct acccagtaga
    26401 tctggcagtg ggtgattttg attcattgtc gaacgaagag cagaaggaag tatttgcaaa
    26461 agccaaagag tatacccgtt cccctgcaga aaaagacgat acagatacac agttaggtat
    26521 cttaaaaaca tttgaacgtt acccgcaagc ggaagtcact ttgattggag ctacgggcgg
    26581 aagattggat catcttttag ccaatctctg gttaggtatg gaacctcgct tcaaaccctt
    26641 tgcccatcag cttattatca gagattgtca aaattatttg tcttattatt tgcctggaga
    26701 gcatatagta agacagatac cagatatgaa gtatttagct tattgctgct tgactcccgt
    26761 ctcgaatctt acattaaaag gcagcaagta tgtattggaa aataaagaag tagaatatcc
    26821 gacatcttat gcaagtaacg aatttctttc agataccgca gaattttctt tttcagatgg
    26881 tatgattgca gttattcaaa gcaaagattg atccataaac aaataaaaaa atttgtcttt
    26941 caggctaaaa atgacagaat ctattatttg ctaaaaaaaa gcttaagagg agcaacttac
    27001 cttttgtttc acggcattta gtgataaaat tgaacacaag agtctaatta attcgaggtg
    27061 tctttgtgaa gaagttcaat cctaataaaa atattattat taccttggtt attgttatca
    27121 tcgtggtgac gatactaagt gtgaccgctg cgcagcgagc gaatgaagga aagcccaatt
    27181 tcttccaatc catcgttaat gacagtgttg caatggttga taaaacaatc tctgccccag
    27241 tacggtgggt acgaaatgga gtagattctg tccataatct tttttcaact tatgccgaaa
    27301 atgagcggct aaaagaaaaa atcgatagct atgatgacgt ttcacagcaa aacaagaatt
    27361 acgagagaga aatcgaagcg ttgaaaaaag aacttgagtt gaatcagacg ctgactagtt
    27421 acgaaaaagt aacggcaaat gtcattactc gttcgcctga cagttggcag gacatgctga
    27481 tcgtagacaa gggttctaaa gacggtatcg aagcaaatat ggcagtcatg tcgcaaaaag
    27541 gattgattgg ccgagtgatc gaggtcaata cggcttcgtc taaaatcgaa ttactgtcat
    27601 cctctaatga aagctccaat cattttccag tacgggtatc ttcggctaat ggcgaagcgt
    27661 ttggtttgct taaaaactat gatgaaaagc tccatgcctt agtggtgacc caattaactg
    27721 gtgatacgga tatcaaagaa ggggatgttg tccagacatc cggtcttgga gggaattctc
    27781 cagctaactt gccgatcggt acggttatta aaacgaaacc agatagttat gggctggatc
    27841 gggaagttta tgtgaaacct tatgcagaaa tgtatgacgt gtcagttgtg acgattgtcc
    27901 aacggctagc ggaggattag tccatgttga agaaagaaac aatgaagtac tatctgccaa
    27961 tcgttttgtt ctttttgatg ttgatagatg gtcatttaac aagaatgcta ggggagtggt
    28021 cgaaaggcac ctatatgtca aatgcccact ttctgatatt ggcattatta tgttgcagta
    28081 tggcgtttga aaaacgttat ttactgatta ccacgattgt tctcggggct atctatgacg
    28141 cttactatat tggcgttatc ggtatctatg cagtagctct ccctttaatt gtatggttga
    28201 tgtatgtaat gaaagacgtt atccatgtca acatctttac tgaatttttc agtatgatca
    28261 tctttgtcac gggttatgaa ttgtttacga tggtggtcca gttgattttt aaattagcag
    28321 tagtaaataa cacgtatttt attacaaggt ttttaggacc tacactgctg ttgaacatga
    28381 ttatatttgt attattcatt tttcccttta agaaattatt cagtgatgaa taatttcttt
    28441 ttttattttt aggtcgaaag cgaaaaagaa tcgttgttat cttaagaaat gctagactta
    28501 acgcggcttt ttcatgatca ttttctgaaa cgaacagttt tgtcattttt aaaagttgtt
    28561 ttgaaacatt tttgtaacat aactattata tcgttgacac actgctatgg tacaattttt
    28621 atatcgttaa gaaagactta aatatattta gatatttatt aaattaagcc agtcggagga
    28681 atgaagagtg aaaaagagtt taatatcagc agtaatggta tgttcaatga ccttgactgc
    28741 cgtagcatca ccgatcgctg ccgcggcgga cgattttgat tctcagatac aacagcaaga
    28801 tcaaaagatt gcagacttga aaaatcaaca agcagatgca caatcacaaa tcgatgcctt
    28861 agaaagccaa gtttctgaaa tcaacacaca agcacaagac ctattagcaa aacaagatac
    28921 attgcgtcaa gaatctgcac aattagtaaa agacattgca gatttacaag aacgtattga
    28981 aaaacgtgaa gatacaatcc aaaaacaagc acgcgaagca caagtaagca acacaagctc
    29041 aaactacatc gatgctgtat tgaatgctga ttcattagca gatgctattg gacgcgttca
    29101 agcaatgaca acgatggtaa aagcaaacaa cgacttgatg gaacaacaaa aacaagataa
    29161 aaaagctgta gaagacaaaa aagctgaaaa cgatgctaaa ctaaaagaat tagctgaaaa
    29221 ccaagctgct ttggaatctc aaaaaggcga tcttttatct aaacaagctg atttgaacgt
    29281 actgaaaact tcattagctg ctgaacaagc aactgctgaa gacaagaaag ctgacttgaa
    29341 ccgtcaaaaa gcagaagctg aagcagagca agctcgtatt cgtgaacaac aacgtctagc
    29401 agaacaagct cgtcaacaag ctgcacaaga aaaagctgaa aaagaagcac gcgaacaagc
    29461 agaggctgaa gcacaagcta ctcaagcttc atctactgca caaagctcag caacagaaga
    29521 atcatcagct acacaatctt caatgactga agaatcatca tcagctacac aatcttcagc
    29581 aacagaagaa tcaacaacac ctgaatcttc aacagaagaa tcaacagcgc ctgaatcttc
    29641 agcaacagaa gaatcaacaa cagtgcctga atcttcagca acagaagaat caaaaacagt
    29701 gcctgaatct tcaacaacag aagaatcaac aacacctgcg cctacaacac catcaacaga
    29761 tcaaagtgtt gatacaggaa acggcacagg aagtagtact ccggctccaa cgccaacacc
    29821 aacacctgaa caaccaaaac ctgtaacacc tgctccagct ccttctggaa gtgtgaatgg
    29881 tgcagcaatc gtagcagaag cttacaaata tattggtact ccatatgttt ggggcggtaa
    29941 agatccaagt ggctttgact gctcagggtt cacacgctat gtttacatgc aagtaactgg
    30001 tcgtgacatc ggtggttgga cagttcctca agaatcagct ggaacaaaaa tttcagtttc
    30061 tcaagcaaaa gctggagact tgttattctg ggggtcacaa ggcggaactt accacgtagc
    30121 gattgcttta ggcggaggac aatatatcca tgctcctcaa ccaggcgaaa gcgtaaaagt
    30181 tggttcagtt caatggtttg cccctgactt tgctgtcagc atgtaagaat gattaatcaa
    30241 aaagagttag cccattctgg actaactctt tttt
//
LOCUS       DACKAC010000031        29573 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.031, whole genome shotgun sequence.
ACCESSION   DACKAC010000031 DACKAC010000000
VERSION     DACKAC010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.031"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(48..1343)
                     /gene="purB"
                     /locus_tag="I0B46_001428"
     CDS             complement(48..1343)
                     /gene="purB"
                     /locus_tag="I0B46_001428"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAR2083738.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1356..2489)
                     /gene="purK"
                     /locus_tag="I0B46_001429"
     CDS             complement(1356..2489)
                     /gene="purK"
                     /locus_tag="I0B46_001429"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAR2083739.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2473..2961)
                     /gene="purE"
                     /locus_tag="I0B46_001430"
     CDS             complement(2473..2961)
                     /gene="purE"
                     /locus_tag="I0B46_001430"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAR2083740.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3377..4684)
                     /locus_tag="I0B46_001431"
     CDS             complement(3377..4684)
                     /locus_tag="I0B46_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAR2083741.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4687..5268)
                     /locus_tag="I0B46_001432"
     CDS             complement(4687..5268)
                     /locus_tag="I0B46_001432"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAR2083742.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5384..5481)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5840..7132)
                     /locus_tag="I0B46_001433"
     CDS             complement(5840..7132)
                     /locus_tag="I0B46_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAR2083743.1"
                     /translation="MKKKYVQVGLGGRARFFYETIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7129..7752)
                     /locus_tag="I0B46_001434"
     CDS             complement(7129..7752)
                     /locus_tag="I0B46_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083744.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7755..8591)
                     /locus_tag="I0B46_001435"
     CDS             complement(7755..8591)
                     /locus_tag="I0B46_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAR2083745.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8608..9495)
                     /locus_tag="I0B46_001436"
     CDS             complement(8608..9495)
                     /locus_tag="I0B46_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAR2083746.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9511..10794)
                     /locus_tag="I0B46_001437"
     CDS             complement(9511..10794)
                     /locus_tag="I0B46_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAR2083747.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11477..12160)
                     /locus_tag="I0B46_001438"
     CDS             complement(11477..12160)
                     /locus_tag="I0B46_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAR2083748.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12178..12795)
                     /locus_tag="I0B46_001439"
     CDS             complement(12178..12795)
                     /locus_tag="I0B46_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAR2083749.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12805..13629)
                     /locus_tag="I0B46_001440"
     CDS             complement(12805..13629)
                     /locus_tag="I0B46_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAR2083750.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13622..14182)
                     /locus_tag="I0B46_001441"
     CDS             complement(13622..14182)
                     /locus_tag="I0B46_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083751.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14356..15312
                     /locus_tag="I0B46_001442"
     CDS             14356..15312
                     /locus_tag="I0B46_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAR2083752.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15617..16573)
                     /locus_tag="I0B46_001443"
     CDS             complement(15617..16573)
                     /locus_tag="I0B46_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAR2083753.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16677..17279
                     /locus_tag="I0B46_001444"
     CDS             16677..17279
                     /locus_tag="I0B46_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAR2083754.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17367..18866)
                     /locus_tag="I0B46_001445"
     CDS             complement(17367..18866)
                     /locus_tag="I0B46_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAR2083755.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18882..19406)
                     /locus_tag="I0B46_001446"
     CDS             complement(18882..19406)
                     /locus_tag="I0B46_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083756.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19704..20453
                     /locus_tag="I0B46_001447"
     CDS             19704..20453
                     /locus_tag="I0B46_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAR2083757.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20444..21229
                     /locus_tag="I0B46_001448"
     CDS             20444..21229
                     /locus_tag="I0B46_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAR2083758.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21198..21914
                     /locus_tag="I0B46_001449"
     CDS             21198..21914
                     /locus_tag="I0B46_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAR2083759.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22016..23035)
                     /locus_tag="I0B46_001450"
     CDS             complement(22016..23035)
                     /locus_tag="I0B46_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAR2083760.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(23054..24112)
                     /locus_tag="I0B46_001451"
     CDS             complement(23054..24112)
                     /locus_tag="I0B46_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAR2083761.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            complement(24333..25481)
                     /gene="nagA"
                     /locus_tag="I0B46_001452"
     CDS             complement(24333..25481)
                     /gene="nagA"
                     /locus_tag="I0B46_001452"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAR2083762.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(25557..26393)
                     /locus_tag="I0B46_001453"
     CDS             complement(25557..26393)
                     /locus_tag="I0B46_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAR2083763.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(26577..27794)
                     /locus_tag="I0B46_001454"
     CDS             complement(26577..27794)
                     /locus_tag="I0B46_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAR2083764.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            28177..28260
                     /locus_tag="I0B46_001455"
     tRNA            28177..28260
                     /locus_tag="I0B46_001455"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28211..28213,aa:Leu,seq:gag)
     gene            complement(28500..29498)
                     /locus_tag="I0B46_001456"
     CDS             complement(28500..29498)
                     /locus_tag="I0B46_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083765.1"
                     /translation="MGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEINRKIYWVS
                     IPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELIFSLTTPGN
                     ALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLIFLLTYRKC
                     QNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLS
                     LFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVW
                     ASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         8845 a         6303 c         5063 g         9362 t
ORIGIN      
        1 ttatttttgc tattctattt taattgaatt ttgcaactca ctcttcttta ttccaaacct
       61 actcgtttga atatcacgtc aacgtttttc aaatgatgat gataatcaaa ggcatcgtct
      121 atttcttctt tagataatac cgatgtgatg gttggatctt cttctaacag cgggcggaac
      181 tgcacttggt tgtcccaagc gtacgcagtt tttggctgaa caagatcata agcttcttct
      241 cttgtcattc ccttatcgat caatttcagc ataacgcgct ggctataaat caaaccaaat
      301 gttgcagaca tattccgctt catgttttca ggaaagaccg ttagattttt aacgatattg
      361 ccaaaacgat ttaacatgta attcaataaa atcgtggtat caggcaagat gattcgttct
      421 gctgaagaat gagaaatatc tctttcatgc cataatgtga cattttcata ggcagtgacc
      481 atgtgtccgc gcaccactct ggctagacca gccatatttt ctgaaccgat cggattcctt
      541 ttgtgcggca tagcagaaga acctttttga cctttcgcaa aaaattcttc tacttcacgt
      601 gtttcggatt tttgcaatcc gcgtatctct gttgcaaaac gttcaatact tgtagcaata
      661 agcgccatcg ctgagaaata ttcagcatgt agatcccgag ggagaacttg agtagagatt
      721 tcttgtggac ggatccccaa ttgtttacat acatactctt caataaatgg cgggatattt
      781 gcgaacgttc ccactgctcc tgagattttt cctgcttcta ctccttttgc cgcatgttca
      841 aatcgttcga tgttccgttt catttctgaa taccatgtgg caagtttcaa tccaaaagta
      901 gttggttcag catggacacc gtgtgtccga cccatcatca ctgtatattt gtgttctttt
      961 gctttttcgc caatgatttc cgtaaaacgt ttcaagtctt cccgtaaaat atcatttgct
     1021 tgcttgatga gatagccata agcagtatct acgacatccg tacttgttag accataatgg
     1081 acccattttc tttcttctcc caatgtttca gaaaccgctc gtgtaaacgc aacgacatca
     1141 tggcgtgtta atttttcgat ttccaagatc cgatcgatat caaatgatgc atgctcacga
     1201 atttttttca catcttcttg cgggatttca ccaagttctg cccaagcttc atctgctaaa
     1261 atctcgactt cgagccacgc acggtatttg ttctcttccg tccagatatt ccccatttca
     1321 gggcgtgtat aacgttctaa catgtttttt ctcctctaat cccaaatatt cgtttgtttg
     1381 atttcttcca gcgtttgctg gatgtcttct gtcaagatag tgatatgtcc catcttgcga
     1441 cctttttttg cttgtttttt tccatagaaa tggaattgcc agtctggctt attctcaatc
     1501 aagcgatagc tttccagcag ttgttctccc aaaacattga ccattactgc attgcttatc
     1561 aaacgtactt tttctcctaa tggccaacca caaatacctc gaatatgggc atcgaactga
     1621 ctcatagaac aagcttcaat agaataatgt ccagaattgt gaggacgagg agctaattca
     1681 ttcacatata gcccgcctgt ttttgttaaa aacatttcta cccctaaaac acctgaaagg
     1741 ctcactgctt ctgcaatcac acgggcaata cgctcagcct cctcgatcac atcctgatca
     1801 atcgcggctg gagcaatcgt ttcatgaaga atattgttgt ggtggatgtt ttccacgacc
     1861 ggaaaagtcg tgaaatctcc ttgaccattt cctgccacca tgatcgaaat ctctttttca
     1921 aacgggatcc atgcttctaa aacacaggtt ccttctctta aaaggtccat ggcaggagct
     1981 agatccgccc gacttttcaa aacatactgc cccttgccat catagccgcc tcgagtggtt
     2041 ttcaaaacac aaggatagcc gatcccgtca atcgcatctt ggatatccgt cggactgacg
     2101 atcgttgcat aaggagcaat cacgatgtta ttcgtttcca aaaaagattt ttctaacagg
     2161 cggtcttgcg taatcgcaag caagtctgtc ccttgaggaa taaatgacat gggtaaaatc
     2221 gcatttaacg cctctacact gacgttttcg aattcatagg taatgacatc tgttcttctg
     2281 gcaagctctt tcagcgcaaa ggtgtcatca tagacacttt caatgtgcca atctgcaact
     2341 tgtgcggctg gacaatcgct cacaggatcc agcacaccta ctttaaagcc catttctttt
     2401 gcactaatcg ccatcatacg acctaattga ccgccaccga cgatcccgat cgttgaacca
     2461 ggcaacaaag gtttaaccaa gttgatcact actttctata acagttttag ccaacattga
     2521 acgtctttca gccaatttag cctccaattc taaatcatac atggaaagca tctgtatcgc
     2581 aagtagtcca gcattaattg ctcccgcttt tccgattgct gtagtagcta caggtactcc
     2641 tccaggcatt tggacaatcg aaagaagcga atccaaacca tttaatgttc gtgattggac
     2701 cggtacaccg ataaccggca aagttgtttt tgatgcgacc atacccggca gatgcgcggc
     2761 tccccctgct cctgcaataa tgattttgat tccttttttt cgagcgtttt gtgcatattc
     2821 aaacatcaaa tctggggttc gatgagcaga aacgaccttt ttttcataag acacaccgaa
     2881 ttcatccaat atttgacatg cttctttcat tgtttcccaa tctgaagtac tccccataat
     2941 cacagagatt tttgctgtca ttgtttttcc tcccagtttt tctacattca tattttaaca
     3001 atcacttttt ttgatgtcaa agaaatttcg aatgttaaca aagaaaaaca ttttattgtt
     3061 cgtgtttatt aaagataaaa aaaaggaatg tgacaaaagt taggtcacat tcctttttat
     3121 ggataaacgg tgttctcctg ccaactcctc ggtcacaagg aacaatattc aacaaacatg
     3181 agaagctttt ttttgaaatt gttccttgtg actcggagat gaacggcagg ctcacgacct
     3241 cttccttcaa cggtgttcaa tcagctgacc ctcggtatta gctcaaacaa acggcaaaat
     3301 acgaggaact atttttccgt ttgtttctct aacaatcagg tcaaaacgct gatttcacaa
     3361 cctttctttt gctctattac ttcttgaagg cttcttccaa tatttcttca tcttttttca
     3421 atccattaaa gatcaaattc aatatgatag ccgtaatgct actcattacg atgccatttc
     3481 cagtgaacat ttgcaatgtt tgcggcattt gactgaataa tgtcggcgta acattgaaac
     3541 ccagcccaaa tccaacagaa acagcaacaa tcaatagatt tttatcattg ctgaaatcaa
     3601 cttccaacag catacggatt ccttggactg ccaccatccc gaacatgact aacattcctc
     3661 caccaagtac cggctcaggg atgatttgtg ctaacgcacc gattttaggg aataatccta
     3721 aaacaatcaa gaagaatgca gagaaataga tgggttttct tgttttgatt cccgaaagtt
     3781 gaaccaatcc gacattttga gaaaagccag tataagggaa tgtattgaaa ataccgccta
     3841 agataacggc aagtccttct gcacgatatc cctttttcaa ttcttcttct ccaacatttt
     3901 tcccagtaat gtcaccaagt gcaaaatata cacctgttga ttctaccatg ctgaccatgg
     3961 aaatgatgat cattagtaaa atcgaccata cgtcgaaagt tggtttacca aaataaaagg
     4021 gttgtggaac atggaacaac ggcgcatctc caatcgcgct cagatcaatc attcccatga
     4081 aagcagctaa gatgcttcct ccgactaaac caatcaatac cgcgattgaa cgaatgaacc
     4141 cttttgccag catttgcacg cctacaatta ggagaatcgt cacaaatgca agtagcagat
     4201 ttgttttgtc accaaaatca gctgctgttg cattacctcc acccattttc tcgattgcta
     4261 caggaatcaa agtcaaacca ataacggtga taaccgttcc tgttactata ggagggaata
     4321 gttttttaat tttagaaaag acaccagaaa ctaacacaac aaagatccca gaagcgataa
     4381 tagatccgta gatagctcct actccttcat tggttccgat caagatcagt ggagctactg
     4441 cttggatcgc acatcctaaa acgacaggca agccaattcc gaaaaatcgg ttaactgtca
     4501 attgcaataa tgtcgctaat ccacacataa agatatcaat tgaaatcaaa taagtcatct
     4561 gttcttgatt aaagcctagt cctgtaccga tcaaaagagg caccgctact gctcctgcat
     4621 acatcgctag cagatgctgc agccctaaaa ccgcagcttt tccattttgt gtttcttttt
     4681 ccacgattat gcgtcctcct caagaaactc aacttgtcca ttcgtcaagg aagcaatacg
     4741 cgcaagagaa acgactctca atcccatttc ttcaagtaac tgacgtccat cttggaaaga
     4801 cttttcgatg acgatcccga ttccttctac ttgcgctcca gcttgctggc ataattcgat
     4861 caatcctttt gccgcttgtc cgtttgccaa gaaatcgtcg ataatcaata ctttgtcttc
     4921 tgaagataaa aatttacgag aaatagaaat cgtacttgtc acttgtttcg taaaagaata
     4981 aacagaagct gtcaataatt cttcatccat cgtcaaactt tttgcttttc tagcgaagat
     5041 cattggaaca tctaattctc ttgcagcaaa taaagctggt gcgatcccag aagcttcaat
     5101 ggtcacaact ttcgtgattc cttgttcttt aaatacttct gcaaaccgtt ttcccatttg
     5161 ttccatcaat acagggtcta cttggtgagt gacgaagctg tcgactttta atacgccctc
     5221 gcctaatata cgtccgtctt tttgaatacg ttcaactaat tctttcactt tatttgctcc
     5281 tttatcattt attaataccg aaataaccgc ttttgtgcaa aaacctacta tgtttctgtt
     5341 aaagaaaaac accctccact ttatcataag aaaagaaggg tgaaatagcc aatcttttac
     5401 ttatagtcca gcatttacgg tactggctag aaactgtcat ccatattatg acgatatata
     5461 agtaggtatg taaaactaat aatagtgtaa attcattttc ttgcaactgt tttgcacgtg
     5521 aaggcagttt gccatatttg aataaaaaat gttgtttcaa atattgaaaa cagtctaaca
     5581 ctactttctg aaactgacaa cgtggtttac ttattttttt gtaaaatcta atgattttta
     5641 atgcattatt cctttttgtt cgggtttaaa tagttatttt tattttctcc tatctaaaaa
     5701 agcagcccac ataaaaagaa ggcatgacaa aaagcatgtc atagcccctt tttccgaata
     5761 aatagtgttc aaaagacagc ctatcggcaa taaggcaaga ttttataaac tacgggaagt
     5821 aatttccgta aatctattct tattctttta agctgacaag ctcgtcaatc actactgctt
     5881 ttcctgaagc aatagaatga tttgctgcga ttcctgtcaa aatcgacaaa ataccatcta
     5941 cgtgagttgc tgcccgattc cacggatctt cttctttttc ttcaccaaat agatcatcta
     6001 ataaacgcgg atctcctccg ccatgtccgc ccgcttcttg ttcgacgatt acctcatatg
     6061 gctcaccgaa catcggatag acatggatgg actgaagaac ggctgctcct tcatcctttt
     6121 tatctccacc tgcattgatg taagactttt cgatcacttt catttcgatc cgtcctttgc
     6181 tgccattgaa agaaacattg aatccttccc acggcatata cgcgtttagc gaatacgtca
     6241 aaatcgcacc agtttcatat ttgaccgtta cgcctagtgt atcttcaata gagatatcat
     6301 cggaaaatac actgcggtca cgaatatatc cactgtcttt ttctgcttct aaatacattt
     6361 ctcgcaattc ctcattggct gacaggtcta tagcaaatgg atcagcttta gctagttcgc
     6421 ttccatagca tcgactgtaa aatatttcta ctccgcgttt ctccgcatta tgcatcccat
     6481 aaaagtttaa tccaccgaat gcaaatacct tttggggacg gctgttcaac caaaaattca
     6541 ctagatcaaa atgatgggta gatttatgaa caagaagccc gccgctattg tgcttatttc
     6601 gatgccatct tctgaaataa tctgccccgt gttgcgtatt taacagccat tcgaagtgga
     6661 tagaaaacac atcaccaatc acatcacttt ctatcagttc ctttatcttc gtgtgatacg
     6721 gtgcataacg ataattaaaa gtgactcgca ggcttcttcc cgtctgtttt tggaccgtta
     6781 ttatttcttg agctttttcc tcatcgatag tcagaggttt ttcgcaaata acatcatatc
     6841 cttttttcat tgcacgaatg atataatcat gatgcgttcg atctactgac gtaataatga
     6901 cttcagccgg ccgtacgtca tccagtagtt gctcaaattg tgatggcata catgtcttta
     6961 cttttttctg tccatacttg ttcacaagta tccgatttgc ataatccatc cttgtttgag
     7021 atacatcaca aaaaccgact agttcacaag tttgatgata cgtagaggca attgtctcat
     7081 aaaaaaaacg cgctctccca ccaagtccga cttgtacata ttttttcttc atttaaagtc
     7141 attctccttt tcttttaagc aaaatactgt gacaaatggc agccctggat acagtaatgc
     7201 agtgagtaaa aaatcatgct gtatacacca gaacagtcca tacaaaaggc ataaatacca
     7261 cagaaacccc ttgacgtttt ttgacaacag ccagatattc cttttgatca aataaacaag
     7321 actatctgct ttatcttctt tgcagatatc aattagcgta ctaaaaaaac tatgggatac
     7381 aaaaagcaac agacacaata gaaaaggaat cagataccaa gctccaatct ttcccactaa
     7441 taaataccaa tcaactgcaa aaataatgac aaacagctga aacaacgtaa tgatgaaaca
     7501 catccgtttg atttttcctt tcctccaaca tacctttttt tctgccaagt aatggacaga
     7561 tagataataa gaaggataca tcatcaaaaa ataaatcaga tagacaacac gagcagacag
     7621 cactaatcct aaagtgaaca aacatcctag aactaaagcg aaaagagcat tactgataag
     7681 actggttaaa aaaatccgac agacatagta gttgagcttg taaagaaaca tctcttctat
     7741 tggctttttc atattcagcc cttcagaccg cttgtactga tcccctggac aagatattta
     7801 ttgaagaata agaaaatcag aaaaaccggt agcagcgaca acgtagacat cgcaaacata
     7861 ctcgcgtaag tcgtactaga tccagcgtct gcaaataatt tgatggcaat cgataccgtg
     7921 taagatctcg gcttgtttaa ataaatcaat ggtcccatga agtcgtccca tttccaataa
     7981 aactggatga tgatcgtcgt gatgatcgaa ggtttcagca aaggaaaaat aattttggta
     8041 aagacactat acttgctgca tccgtcaata atcgctgatt catctaactc tttggggatg
     8101 ctgaccatga attgcatgat ctggtaaatg aaaaatgctt gtccaaaaaa atgtggtaaa
     8161 atcagcggca catacgtccc aaccaaattc agccgattgt aaataataaa ctgcggaatc
     8221 aaaatcactt gtgcaggaat catcatcgtt actaacatga cgacgaacca gaatcttttt
     8281 cctttgaaat cgattctcga taatgcgtaa gcaatacaag tcgagctcag aactgtccct
     8341 atcgtcgaaa ctgcagagat gacaaacgaa ttcctaaaga aagtaaaaaa tgaaattcca
     8401 gcaaaccccg cccatccttt tgcgtaattt tcccattgaa aagtctctgg aatcaaattc
     8461 agcgaaccac tcataatctc ttgatccgtt ttaaaagaac ctgcgatcat ccataagacc
     8521 ggataaatca tgactagacc taatagcaaa ataaaaccgt attgaaacca tttcgttact
     8581 gtattattca tttcttcctc ctctcattca cacaacgacc catttttttg atgatttgaa
     8641 aatcagaaag gtgacagctc ccataatcaa cagcatgatc cacgaaatag cactagcata
     8701 ccccatagaa ttaaaagaaa aagcttggtt atacacataa agagcataaa agttcgtggc
     8761 atcattcgga ccaccttggg taatgataaa tgcctgcgta aaagtcataa acgccgcgat
     8821 tgtctgcata accaagttat acaaaatgat cggcgaaaga agaggtaacg tgatcttgta
     8881 aaacgtctgc catttgttcg ctccatcgat ctctgccgct tcataatacg agcctgggat
     8941 ctctttgata cctgcagcaa agatgatcat tgccgaaccg aattgccaag cagacatcaa
     9001 aacaagaggg atcatgacca gtttttgatc gctaaaccag gaaagcgcag gtgcgcctaa
     9061 tcgttccagg aaaatattca ctaacccgtt tttagaaaac aactcccgcc aaactagagc
     9121 gatagaaata ctgccaccga tcaaactagg cacataatac aaagaacgaa agaaagtcgt
     9181 tcctttgata ttcctcgtta aaagataagc aatcaataga ccaaagatca gtttcacagg
     9241 tacagatgca aatacataga gaaaagtgac tcggatggag ttcaaaaaac gggcatcact
     9301 cagcatcttt atataattat ccaagccgat ccatgagact ggatttccca aacgataatc
     9361 ggtgaacgaa taataaagtg acaagatcat cggtatcact gttatcaata gaaatcctag
     9421 gatgaatgga gaagcaaata cgtaaccgac cacattgtct tgatccaaga accgttttag
     9481 tttttctttt ttcatgcctt cctcctgctg ctatcctaga atgtttgacg caaactcata
     9541 aagactctct gctgcttctt ctgctgttac ttcttcataa atgacttttt caactaaaag
     9601 attgtattgg tcttgaattt ccggattcaa agcactatca aagacattca cttcgccgtt
     9661 gtccatatcg gaaatcattt ctaaataatc aaatgtcgct tgcaaagttg catcatctgc
     9721 acgagcgagc tgttctcgaa tattcgacat gattggtaca ccacgttctc cttttaaaat
     9781 ttcattggct tcttcactgt tttgaaagaa atcaataaac ttagcagctt cttctttatg
     9841 ggctgagctt tttgccatac tcagcatttg cgaagaacgt aaagggatac cagattctcc
     9901 atctgctttt ctgcgaggtg gattaatcaa ctgaatctcc atatcttcag gagcccctga
     9961 caatatttct gtcaactggt tacttgatag aaacaccatt gccgcttctc tatccctaac
    10021 cgcctgaaaa gacagatccg tcgtacttgc agctccacca cctggttctg gatgggcccc
    10081 tttgtctacc agatttttac gcagctggaa aaaatctgtt agatagtctg gattttcaaa
    10141 tcccaagctg ttagatagat cttcccgatt ataaaaattc aatcctttct caacttgtgg
    10201 tatttgaata atacagccag ctataaaatc ttcgaatctt gaaatagcat agattcctaa
    10261 ttcttcagtg atttgatagc tgatttgttc aaattcatcc catgtccaat ttgaagctgg
    10321 ttcagggata tttaattgtt caaatatatt tttattgtaa gcaatcgcaa acgaattcac
    10381 cccgttagac agtccaacta attgcccgtc gtaagtcgtc gttgctaaat aggattcatc
    10441 tgcattagaa atatcaatta tcccttgctc cacatattca tctaatggtt caatctgatt
    10501 gatgtattga ggaaattgat tgttcagttg gaaaatgtcc cagatatcat ctgatgctgc
    10561 tagtgtacct aattttgtgt aatatccttc ataatcataa aattcataat cgatcgtcac
    10621 atggggattt ttttcttcat aaagctcgat tacttttact gttgcatcgt gtcggacttg
    10681 cgaaccccac caagccatgt tcagcgtgat ttttttgtca tcttcttgcc cacaagccgt
    10741 caagaagaat aacataacac ttaaagctaa gatgattctc attcttttct tcattctctg
    10801 cacctctact tccattgtta tttttcaata tactgtattt ttatacagaa catatttatt
    10861 aaacgatctt tttcaagggg aataaaaacc aagacttgcc ttatcgttct gcctaggatg
    10921 aacaactcaa tactttcaag aaaaattaga tagtgctgat tcttctttca tcgctaaaaa
    10981 cacttcttgc ttcaatgatg ggaggataat tacgcaaaga aaaaaggata tcagacaact
    11041 gcccaatatc cttgacatag ctaaataagc atgcttcttc gtgtgcgctc ttccctaaaa
    11101 aacagaaaaa taactagtaa ttctgtatga aattaggtac aatatacacg tcgatgcaca
    11161 agcattgact atggcaactt gattggtcag ttcccgctga cttttcaagc cccgacaatt
    11221 tagctgctac taaattgtcg ggtgcctttt ttagttttta ttcgtttttc attctataga
    11281 aagcgcttca ataaatcaag acattttttt gttagaatgc taataataac aaaaggtttt
    11341 aactcttctt atgtacatta caggtaaaaa aacaaaaaaa tttctctttt ttttgaattc
    11401 tgaacaaata gtactggagt acaaaagacg aggaagagcc actatctgct cttcctcgtc
    11461 ttttgattct cacctgttat tcatgattct ttttctcagt ttcccgatat ttttccattt
    11521 tacgactaaa aagctctccg ctacttggtg gcgtatatgt gatggcattt gctcctgctt
    11581 gaatggtctc agcaatactt tcttccgttg gaccacctgt cgcaatgata ggaagttcag
    11641 gatatttttt acgaaaatgg cgtactgctt ctgcagtttc tttacctgca ctgatattga
    11701 taatcgaaat accagctgct aacttttcat ctatttctgt atatttcgac gttaccgtac
    11761 tgataactgg aatatcaact actcgacaaa cttcttttac tgtctctaaa ggcgtaggac
    11821 cgttcaaaac gacccctaac gaaccttgcg cttctgcgaa catgctcatg taggaagaac
    11881 gagcaccttg tgtcaaacca ccacctacac cggagaaaac tggaatgtca gctgcctcaa
    11941 taatgctttt agtaatcgct ggatgaggtg taaaaggata cacagcaatt actgcattcg
    12001 catttgtatt acgtatgatc gcaatatccg tagtaaagat gatcgaacgg atcaattttc
    12061 caaatatctt gatcccgctt gcttctaaaa tgatttccgg tactcttacg atatcttttc
    12121 tcaattccgt catgatttct ggcgtaaatt gttctgccat cagccacttc tcctttgcta
    12181 tctccggtca tactgattca attgatacgt ctcgatgacc tggatgattt tttgcgcata
    12241 accggggtct gtcgcataac ctgcatcttg caatgcttgt gcggcttcct gatagttggt
    12301 ggccgtaatc actttttcat acagcgcagg attccaatct actccttgga cgaacagttg
    12361 tgtatggtca tccatggact gttcccatga atcatacact ttgaagtctc cttggatagt
    12421 tatccattct tcgttaacaa attcctttgt ttccaggctt actttcttct gatccccata
    12481 agccttgatc ccgaaaagat tattgtattt gctagccaag gtactttttc cccaatttga
    12541 ttctaatata gcttgtccta aaatgatact aggaagcact ccataaccat cttgtagttc
    12601 ttgggcatgc ggaacaagcc gttcaataaa ttgctgatgg gattgcgctt cattttcttg
    12661 ctgattatca gtaaaaggag aagacagatt cttcaaggaa aaaacaaagg ctaagagtat
    12721 cactgctaca ccggcaacca ctgctggcca acggatattc ttgcgctttt gctttttcca
    12781 cttttttttc gccatgatca attcctactt ttcatttaat tcattcagat attcttctag
    12841 cgtcaattgg tgtttggtga tatactcagc gttttcttta ccgacataac gtaagtgcca
    12901 aggttcatat gtgatcttag ttatatcttc gtgtcctttt ggataacgaa cgataaatcc
    12961 atactgcgca gcattatctg cgatccattt agctccaggc tgatctccgt aacttgcatc
    13021 caatacagta gaaggataac tgttgtacca gttttgatcg acgacatcga ctgccagacc
    13081 tgtgtggtgt tcgctgtatc ctggctccgt aatcgtttct ttagtaatct ttgtcgcctc
    13141 ctcttctgat acaccttgac gactcatcac atcttggaca ttttgttcga aaacttgttg
    13201 ttgggatgat acagaccggt aagctgagac catcactaat ggaaatccag ctgcttcagc
    13261 agccttggca aatgcttcat attctggagc aatgcgtttg tcgatcaagt atccgttact
    13321 taatgatgcc aattgagtag actcatcaat ttctttttct aatttgtgat ctggaccaac
    13381 caaaactagc ttccaatcgt ctgttttaac atctggcaat tgtttggacg tacgatcttt
    13441 tgtttctttg tcctttacct tgctgctggt tgtttgactg ttcttttctt tactagcagt
    13501 tgttttggtg cttgttcgtt cgactgcttg attgccggct tgaacatgcg aatcatcatc
    13561 gctaaaaata acatagccca ttgctgcgat cataatcaca actgctccaa atcctaaaaa
    13621 tttattcact tgaaaaatcc tttcgttgtt cttcacgttc cccattcgcc tcatgaacgt
    13681 aagtttcttc cagattttca ttcacccaat cgagaagttc gattttttct ccttcgatct
    13741 gtttttgaac atctgctggc agacgaaaat tcaaaatatc atcatagccc cattcatcat
    13801 attgaatact tactttcaag tcaaggtatc taccagcctg ctgattattt ttcaaaggga
    13861 tcagctcgac ttttgctgct ttgctgctaa gccatttttg cgcgatgcgt gttttgattt
    13921 gcttgtactc tgcaacggca acgtttcgtt tttcgggaaa aataatcgtt tgacgcaaaa
    13981 tcttttgcgt tagcatccat tcataatcag tgaaaagatt ttttactttc tgcatcgctt
    14041 cagatggcag atttttctcg ccatctttcc atttttgcca ttgttctgga ctgatcagca
    14101 agtattcatc taaaaaattt ttttcagttt caaactgttc taataataca ctcataataa
    14161 gatcaatata tacttgatgc acgtttatcc cctcctttta actattctac ttaagttttg
    14221 atcaaaaagc acctcttaaa tcgctgcttt catttttttt aatgtttcaa gtcctctcct
    14281 ctttattctt cgtgattttt gttataataa tagacagaca agaaactcaa tccaactatg
    14341 aaggagcgtt ttcagatgaa aaaatatcaa tggggaatcg ttggtcttgg taccatcgca
    14401 catgagtttg ccgaaagctt caatcaagag acgagcgaac taacagcagt tgcttccaga
    14461 acttcagaaa aagcggaaaa cttcgctcac cgttacaata ttccaaaagc ttacggaagc
    14521 tatcaagaaa tgttagacga tgctgaaatc gacatcgtgt acattgctgt accaaataga
    14581 cagcatatcg accatatctt ggcagcattg gaagcaggca aacacgttct atgtgaaaaa
    14641 gccatcacga tgaataaaaa agaactggct gacgctatgc gcttagcaga agaaaagaat
    14701 ttgatcttag cagaagcgat gacaatcttc aacatgccac tttatcagca gttacgctca
    14761 ataatggata caggaaaact gggggcttta aaaatgatcc aagcaccttt cggcagttac
    14821 aaagaaccag accctaaaaa ccggttcttc aatccagaat tagctggagg agctctgttg
    14881 gatatcggta cgtatgctgt atctttcgct cgtttctttt tgagctctca acctgaagta
    14941 gttgcctcta caatggttcc ttttgaaaca ggcgtggacg aacaatccgt cactatcttg
    15001 cgtaacaaag aaaatgaact tgctgctgtc agcttgactt ttcaggcaaa aatgccaaaa
    15061 gtcggtatcg ttgcctttga gaacggttat gtgacgattg ctgattaccc gcgtgctgat
    15121 cgtgcggaga tcttatttac agatggtaca aaggaattca tcgaaagcgg gaatacatct
    15181 gaagcaatga attacgaaat aaagaatatg gtacacatga ttgaaggtga cttgcccaat
    15241 cgctctctct tcttgacaaa agatgtcatc gaaatcttgg atcaaatgca agttctatgg
    15301 aaaaatatgt aattttaaaa ggttgtgaaa aagctgttct gcatcaagta ataagaacag
    15361 ccaaacaaaa atagctcctc ctatttttcg tttggctgga cttattaccg cagaggaaag
    15421 cttttgaaca ccgtttaaga aagaggttgt gagcctaccg ttcagctccg agtcacaagg
    15481 agcaatttca aaaaacagct tctcatgttt gttgaatatt gtgacttgtg accgaggagt
    15541 tgacaggaga acaccgttta aagaagaggt tgtgaaatca gctttttgac ctgaatatta
    15601 gctgaattca caacctctat ttttggattt tttgttggga gaattctgcc ttgatttcaa
    15661 taaaactttt aaaagactgc aaaaactgga acaattctgc tcgatcctcg aattcagggc
    15721 gactagtcgg cagaggcatt tgccgataca atacataaac ttcttcaata cgtgttaaaa
    15781 tttgtctgcc atcatttgac tctgaaaatg tttctgcagt ataaatcaat aaaccataga
    15841 tatgttttcc ataataagct ggttgctgga ttctctctag attttccagc atgtctttaa
    15901 tcacattggc ctgtgctctt ctcatagaga agtaagtttc gtagtatagc ggctgtctga
    15961 gccagtagtt ttcttgatgt tctctggcat cttcttggct ttcgcgaatg aatgtcagta
    16021 aatgttcgca tttgatctgc acatcttctt tattttctga taaaagaaat tctgccattt
    16081 gtctcaaaat atttcgaaat tctttttcga tctgtatttg gttttccttc agctgcttcg
    16141 tacgatctgg catgtaaaca tttgcaagca aagcaaatac taatccaatc gccattaatc
    16201 ccgcttcatt gccgatcagc tgccaagaca tgttttcctc cactaaataa tgagtgacca
    16261 aaaccgaatt tactactatc ccttcggtta atttgaagcg agaggcaagc gggatgaaca
    16321 ggaataaaaa aagactgaag ccaaaaatag tatacccaac agcaaaaaaa ctgaacagtg
    16381 ctgttgccgt agctaatata aaggctgcga ttcgattcat gcccgtacgc aatgtagagc
    16441 gcttggtatt tcctacactt aataatgcaa tgatccctgc agctggccaa tacaacagcg
    16501 ataagctacc cgctgtgacc atcgaaaaga agacactgat cactgttttg actgttcgca
    16561 ttcctatacg catttacttc cccctttttt attttaatta tacttgaata atgaaaaact
    16621 agctatcaga caaaagttta ggtatgatag taaaaagacc aaaaaggaga atctctatgc
    16681 aatttggccg ttttcgacta ggtatgcgta cgatcaaaag tgccttagct gtttttttat
    16741 gtatcttgtt ttttcatttg actcaacgag gaatcccgat gattgctgca ttatctgctg
    16801 ttttttcttt gcgtcaggac ttgactacaa ccgtttcttt tggaaagtct aggatcatcg
    16861 gcaatacatt aggtggtaca ttagctatca tttatttttt tgtcaaaagc tattttcaca
    16921 atgatttttt ggtagaattg atcttgcttc ctattctagt tattattgtg atcgtcgtgt
    16981 cagatggtat taacaataat tccggtatca tttccgcaat agctacgttg ttgctgattg
    17041 cgttgagcat ccctcaaggc gaatccgctt tgtatgctat ccaacgtgta ctagatactt
    17101 ttatagggac attcattgca atcggtatca atttctttct tcgtcctcct gaaacagaaa
    17161 agaaggaaga actatcagaa gatttggtcg aactccaaaa gaaagaacga ttgctaagaa
    17221 caaatttaca agagatagaa gctaagatcc aaaaacaaaa gaaaaatagc gaggactaat
    17281 tttcttttgc aataaaataa aaaaatggag agaagaactt taaaaagtct tcctccattt
    17341 ttgattgaac ggtgaagtgt actactttat tcttccttca tcgagttttg ttctgcctct
    17401 tttggcgctt catacatatt tacttggcta gtagtcccac gtgtcgacaa attctctacg
    17461 atttccttca agtcaagtcc tgttgtcgct tttagtgttt cttgagtaga agaaagcaaa
    17521 tttgtggcat aatttgtcac tcggttcgct ccactggttt ctcctgaact gcttcctgta
    17581 tcgacaacag agattttttc gatatttcct agcggctgtg ctgcttcgcg catcaattgc
    17641 ggtagcatat cgataaccat actcaagaca gctgcttcgc catattcctt gaaggcattg
    17701 gcgatctttt gttttgcttc ggcttctgct tttccttttg ctagtgctgc ttcggcttct
    17761 gcttgtccag ctaaacgtgt cttattggct tccgcttctg ccagtgcttc cacacggaat
    17821 cgttctgctt cggcttcagt cacttctttg actttttgag cttgtgcttc ttgttcttta
    17881 gcgtaccgat cggcatctgc ttttttcttc acttccgaat catattgctt ttcacgacgt
    17941 gtgatttctt tttcttccag ttcgatttgt ttttgacgtt ctacgatttt aatctgcatt
    18001 tcctgttcga ttacttgttg ttgtgctcgg gcactttcta aattgtaggc ttggtctgct
    18061 tttgctttag caacatcttg ttcttgtttg taggttgcta atttcagttc tttttctttt
    18121 aatgattctg cgatctctgt ttgtcgctgt aattcagctg cttgtgattc tttttctgct
    18181 tgagcttttt tgatacgcgt ttcttttaga gcttccgctt ctgctatatc tgcatctctt
    18241 ttgacttggg cgattcttgg tttccctaaa gagtcaagat aaccgttttt gtctctgacc
    18301 tctttgatag tgaaagaaac gatgatcaac cccatttttg caaggtctac agaagcgact
    18361 tcttgtacgc tttgactgaa cttatcgcga ttttgataga tttcttctac agtcatagaa
    18421 ccaagaatcg agcgcaagtg tccttctaat acttctcttg cttcattttc cagttcttct
    18481 cttgtcttgc ctaaaaactg ttctgctgct gtagcaatct cttctacaga tgaaccgatt
    18541 ttgatgatcg aagtaccatc acacatcacc ggtacgcctt gttccgtata cacttcaggg
    18601 gtggagacgt ccaatttact cgataataga ctgatccgat tcgaacgttg aaaaactggc
    18661 aagacgaatg cgccacctcc acgcacaatt ttgattttgt tattgctttc atcaatgtgc
    18721 acatttttgc ttcctaaata acttccactg atgatcaacg cttcatccgg ttttgctgtc
    18781 tggtacttcg ctacaaagac gatcagcaac atcagtaaaa tcaaagctgc gattgcgatc
    18841 gcgataaaaa caactggtat ttccatttaa cacacgctcc tttaagcttt tatcatgctt
    18901 tcataaggca tgacataaca gattctttcc ttgatctcaa tgatcaatac tttcgttccc
    18961 cgctcagctg tctgttcttg ggggctgtaa aaacaagctg gacgactaat ggaaccggta
    19021 acacttttca actgaatttc tcccatacct cgtacaggga tcggtgtgat cacaacagct
    19081 acttgtcctt ccagagtttt ttcagaagac gacatcgttg attcggcatg tttcatcggc
    19141 atcaatatat agaaattaag caaaaatacc aacactatcg atatgcctcc actagcaata
    19201 aataaaaata aagatgattg atctgttaaa cgctcaccta gaaaaccaaa taagcttgtg
    19261 aaagttaacc aagggataat aagcatcgga tcaagcgggc catcaaaatc gaatacatct
    19321 ccaaaaataa tcaataggac tgccaatcct gcacaaatca gcaatgtata ccaataaatc
    19381 gtctcaagtg cgattccccc tatcatatac acgcctcctt ccatttctac gtctatcata
    19441 ccaatgtttg aaaattctgt atagcttctt ttaccgactc agtggtgttt tttataggaa
    19501 ggttgtgttt atcgttgaca atttgttaaa tttttgtttt catgttaaaa ttttcacaat
    19561 aaaatctttc gaggtagcct atgtttcctt tttttaagaa aaaaccatta aagctccaga
    19621 acacgatcat ccttgtcgtt tgtttctcca cattcgtttc tttgattgtt tctgccatat
    19681 tgatcaggaa ttttattctt tcgatgaacc gatctagtgt gtcagataaa atctctagca
    19741 ttgctaaaat cgttgccaat gataaactca tccaaaaaaa gctgcagact ggtgacccga
    19801 cacatgcgat tcaaggaata acaaatgaaa tcatgattgc atcgaattta gattttatcg
    19861 ttgtcatgga ccctgaaggc attcgatatt ctcacgcaga cccaagttac atcggtcttc
    19921 ctttctcaag tcatgaagat gtgaaaaaag tgattggagg acaagaattt ttctctgaaa
    19981 aagaaggaat gttagggttc ggcttccgct atttcgtccc tatttattat gatcaagaga
    20041 ttattggtgc agtttgtgtt ggcttaacaa acaacacgat tgacaatcaa gtcaaagatg
    20101 gacaaaaaat gattgttttt tcattgatta ttggacttag cgtaggcatc ataggcgccg
    20161 tttttcttgc ccaaaaaatg aaaaaagtac tgcttggact tgaaccacaa gaaatagcag
    20221 aacagttaag tgaaaaaacg attatcgaaa atgaagtaga tgaaggaatc ttagccatct
    20281 cttctgataa aactatcctc ttgatcaata cacctgcaaa aaacattttc taccaaacag
    20341 agcagctatt ggatttcgaa gtaggaaaaa aatcagtgat aatctatatg acgttttgtt
    20401 taaagagacg attgagcatc aaacgaccgt tcggactcaa tccatgtatt taaatgggat
    20461 agaaactatt tctagcgtta cacctctttt tagcaatgac cgttttttag gagcagtcgc
    20521 aactttacga aatcgatcag aagtaatgaa attaatggag gagcttgggg ggacgaaaca
    20581 atatataaac tccatgagag ctcaaaccca tgaattcatg aataaacttc atgtcatttc
    20641 tggtttgatt gatttgaaga agtatgaaga agtcacccat tttatccagc agctaaatca
    20701 aaattataaa aaagagattg gccaagtctc aagtctgatc aaagtacctg caattgccgg
    20761 ttttttatta ggcaaaataa acgaagccaa agaacaatct gttgaaatgc tgttagaaag
    20821 cgattctttt atccctaaaa tggagatgga tgactccatc cattcacttt tgcaagtgtt
    20881 agggaactta cttgataatg caaaagaagc cgtgctacaa aaacatgaaa gaaacggaca
    20941 ggtccggatt catttaaact atgacacaga agctgaagta ttcatcgtga ccgtctgcga
    21001 tgacggtgtc gggatatcag aagaaatctc agaaaaaata tttacgctag gtttttcgac
    21061 gaagggaatc aatcgtgggt ttggattgaa tctcatccat tcaatcgtca aacgccacgg
    21121 tggattttta gaattttcca aacaaacaaa tggcggtaca atcgtctatc tagaattacc
    21181 aaaaaaggaa gatcaaaatg atgacaaatg tcctgataat cgaagatgat ccaatggttg
    21241 cagaattgaa tcgtactttt gtggaaagca tccctaatat gaacgtaatc gaaagtttca
    21301 gaaatgtaga agaaggaaaa aaatatttca gacaaaaaaa cgcagatctg ctgctgcttg
    21361 atgtatacat gcctggcgaa agtggtatcc attttttatc ttggcttaga cagcaaggaa
    21421 atgatatccc agtgattttg atcactgctg cagatgatat cgaaaccata aagaatgtga
    21481 tccgctacgg tgccttagat tatttgatca aaccgttcac tttgcaacgt tttcagctgg
    21541 cgatcgaaaa gtatcttgct ttctctcaga caatccaaac gactgaaaaa gccgatcaga
    21601 aattgatcga tcaagtattc aaccacacca ctcccattca cgaaatacca tcattcatcg
    21661 atttgcctaa aggtctgtca aaactgactt taaaaaaaat aagtgcattt attctaaaag
    21721 aagatactat tttttcaaca aaagaactgg ctcaaaaaag ctcactgtca cgtatatccg
    21781 tcaagaaata tttgaatttt ttagtagaac aagatatatt gaacgaaaga atcaaatatt
    21841 tggaaatcgg ccggccgcta acctgttatg aattgacaaa gaatgggcgg aaaaagctca
    21901 cagattactg ttgagtcatc gaattatccc atacaaaata gtccaagtat acagaagtaa
    21961 caactgttta cttggactat ttttaaacat tttattgacg aatatccatg ttttcttatc
    22021 ttaattgacg ggctttagga agagatacct catttttcgt ttttccagtt tctattattt
    22081 cacgaaagtt ctcgaaactt gtcgaaatca tatttgataa tgcctcatct gtatttgaac
    22141 ctacatgcgg ggttacaaga actctagggt acatttctac taattgctgg atagcagggt
    22201 ctggaatcgt ctcccatggc ttgaaggatc ggaaaaaaac ctctgtttca tttgcaaaaa
    22261 catcggttgc aaaaccagct aaatgattgc ttttcaaagc tcttaaaata gcttcattat
    22321 cttgaagctc tccacgggct gtattaatca agatgctacc ttttttcatt tgagctaaaa
    22381 atccttcatt gatcatttga tcgttttcac ctgggatatg aggtacatga atactgacaa
    22441 tatcgctttt ggctaataac tcttcaagcg agtggaacgt caatactttt ttagcagctt
    22501 ctgtctgatg aatatcgtag ccgatcactt tggctcctag tcccttaaac aaaacagctt
    22561 ccgtcaaccc tatcctgcct gttcctatga tccctactgt gcaatttctg atttcacgac
    22621 taaacatttg ctcatccact cgaaaatctt tgaaagatgt tcggatcgtc gtatacgcag
    22681 tatttctcaa aagcatcatg gcaaaagtca aagataactc tgctatcgca tttggcgaat
    22741 acgatggtac acgggctact gtcataccaa aatcagctgc cgcttgtaga tcgatatgat
    22801 tgactcccac agtccgtgta aaaacataac gaatcccata ttcctgcatt tttgcaagat
    22861 tggtctcatc tgctatacaa tttcccctca acaatacagc atcatggtcc tttgctgaca
    22921 cgatattgtc atgtgtcaac agttcttcat aaagactgag atcataatgg taaatattca
    22981 atttttcaaa aaaatctatc tcgttcggac gaacaccgta acatgcgatt ctcatacttt
    23041 cttcctccat ttcttatgaa aataaaccta tgccatgaat catttgtaat acaacgatcc
    23101 atacaggtgt cagtaccaca gcaaaaatag ttgacagtaa ggatgcattg gaggctaaaa
    23161 gtgcctccct atcaaaactg atagcatagg ctgctgccac tgttgcagct ggtgtagcca
    23221 tcatgataac gattgttgcc agtgcttcaa aattgactgg tagaatattc gttgccgtca
    23281 aaactgctag aatcacgata ttcaatgcag gtacaaatac cactttgact agactgtaat
    23341 accatgaagt tttttcagat gcagcttttt tgaagcttac ttcacctaat gttgcgccga
    23401 ttgctagcca cgccaatggt gaacttaaac cagctaaata atccatcggc tgaaatagcc
    23461 acggtgcagt ctggtcaatt cgcaaaaacg ctacttggct tatctctcct tctgcacctg
    23521 taactgacac ttgtggcaga tacccttgga acagccaaat gaaaaggccg gcaaaagtag
    23581 cgataatgat tggattgacg aacatcgctt tgatattttt agcttccatc ttcaagccac
    23641 tcatttttat gtatccataa gaatacaaaa agattcggta gccaatattg aaaattgagg
    23701 aatacatcac gccgacagga ccataaatcg cactgacgat aggcgttccg aaaaaggtcg
    23761 tagaaccaaa gatcgtcagg acacgcaacg ttgtttgctt atccccttta tagcttaaga
    23821 aaaaaggttt tgaaactaga ataagaataa tataaataac taatccccaa atcaagacat
    23881 tgattccttg catcagtaat tctgtattga tatcttgcat gaaagctcga aatgccagtg
    23941 ctggcaaagc cactgttaag acgactttcg atagaatttt tccaacagat tcagaaaaaa
    24001 tccccttttt tctacaaaca aaccctaata atataataaa aactgttgaa gtgattgcac
    24061 tgacaatcga catatctgtc aaagtcgatc gaaaaatgtc aaataagttc atctttctcc
    24121 tcctaattat gttcatttat tcacaataaa aaaagctttt attttgctaa tacgtattaa
    24181 catttttaat tttatcttta cattgttact tattgaacaa taggaaatta aagaaacttt
    24241 tgaaacttat gtaaagtgat ttttactaga acacaaaaaa aaccttaaaa ccaaacttaa
    24301 gaagtttagt tttaaggtta acttaaatta tattatgcgt ggaaacgttc cacgccatct
    24361 agataagttg cttccaatgt catatcaggg ttcaatacga taaagtctgc atcgcgtcct
    24421 tgagcgattt ttccacatgt atcatcgatt ttacagctga ttgctggaat cagactagcc
    24481 atcatgactg cttgttctgg tgttgctaat ccccagtcta caacattttt aattgcttct
    24541 ttcaatttca atatacttcc agctaagttg ccagattcta gacgtgctgt tccttctttt
    24601 actactactg gaaattcgcc taaattgtaa tttccatcag gcatcccacc ggccatcata
    24661 cagtctgtaa ttaatgcgac atgatcatgt cctgcttttt ccattaggat tcccgcagca
    24721 tttggatgta catgatggcc atcacaaatc aattcagaaa agacatgttc taaagataat
    24781 aacgcaccca ccatacctgg ttcgcgatga ttcaatccac gcataccatt gaaagcatgg
    24841 acaaatacac ttgcgcctgc ttcaacagct ttttgcgctt cttctagtgt agcattactg
    24901 tgtcctagag aaacaactac tccttcttca gtcacttgtt tgacaaactc ttctacacct
    24961 tgacgttccg gtgctaaagc aatttttttg attaaaccac ctgaagcttc ttgccattca
    25021 tggaaagtat ccaaatcagg atccccgaaa taacttgggt tttgtgcccc tttgtgttct
    25081 tctgtgaaga aaggaccttc aaaatagatt cctttgattt tagctcctgg tgcttcctga
    25141 tacattttgc cgattgtctc agctacatct tttaaacgtt ctttgcttga cgtcaatgtt
    25201 gttggtaaaa atgaagtcac tccacaagat aacagacctt cagacatcac tttgatgcct
    25261 tctgcatcat tatccattac atcatgattc atatagccgt gaatatgtgt atctaccaaa
    25321 ccaggagcaa tccatttgcc gctttggtca atgatgttga cttctccctc aggtagttct
    25381 ttggtgtatt caccaaaaag tccatcggta atttcaagat agcctgcatg tttgacacct
    25441 gacgctaaaa agaatttgtc agcatagata aaagttctca ttctattctc tccctttgtt
    25501 tttctacctc taaagtactc ttatttagag aagaagtcaa gaatggtcta caccatttac
    25561 aaattttttg aaatttgttt cacagcttgt gctaagcctt catagaatgc tgctctttct
    25621 ggttcttctt tacgcttgta tattaagctt ttaagcacaa ataaatcatt gaggtaatag
    25681 tagctattct tctcttgagc ccaatagatt ccttgattca cctgttctaa cgcttcgtcg
    25741 atttcatttt gatggaataa gaaatttcca taattataaa aaatttgcga aaattcactt
    25801 tttgatcgtt catcagctga ttgatgcagc agttggatgc ttcgactcaa atagtagcgt
    25861 gcttcagccg tttttcctgc cacaccatat atttttccga tacaactaat cagttgtatt
    25921 tctgtgtcag aaacatggat tctttctttt tggctagagt agcttaatgc ttctttcaaa
    25981 taataaaggg actgagagat attttgacgc aacgtgaatt cgcaaacacc gagataataa
    26041 taatagcgtt gaaagtcagt tgcttgatac agattttcag aaatatcaga agattttagt
    26101 acttgttcta acttccgata atcacgtttt tggaaacaac tatccaacag ttcaaataat
    26161 ttatcgtttg tccgagtagt ttttcgagag agattcatca catgatcaat cgttacccct
    26221 agccgttgac aaatctgcat catcaccaat acattcggca gttcctcatt attttcgatc
    26281 cgacttaata cactttgtga acaaatatca tgcgaaagca ttttttgcgt cattttttgt
    26341 tcttttcgaa tctctttaat gatcgttcca aaggactcga ttaacgtttc cataagtatc
    26401 ctccccaaaa tatgcgtatt cgcattctat tcgtatttta ctttaggaag tcgcaaactt
    26461 caagaaaaga cttgcaacta aataatatac tttagttatt taagaaaact ccactagaaa
    26521 gagagcctca gtgtgtttgt ctgtggccct cctactgaaa aatttggttt ttaaaatcat
    26581 ttttggcgat aggttgctag gaattctctc attttcttag ctgtttgttt caaatcatcc
    26641 attttaggaa gataaacgat tcgaaaatga tctggctggt tccaattaaa tccaccacca
    26701 tgtacaagta gaatatgatg ttcatgtaaa aagtccaaaa cgaatttttc atcatccaaa
    26761 atattgaatc gctttgtatc aatttttggg aatatataaa aagcagcttt tggttttacg
    26821 gctgaaagac caggaatatc attgattgca ttatagatat attctctttg ttcatagatc
    26881 cttccgcctg gcaggagcag ctcatcgaca ctttgataac cgcctagcgc ggtttggata
    26941 atttgctgag acaatacatt ggaacacaaa cgcatggagg acaacatatt cagcccttct
    27001 atatagcctt tgacgtgtga cttatctcca cttaggacca tccagcctac tcgaaatcct
    27061 gctacacgat gagatttgga tagaccgttc agcgtaacaa cgaacaaatc aggcgctaat
    27121 gtagcaatcg gaatatgttc tagaccatcc atcactaagc gatcatagat ttcgtcggaa
    27181 taaataatca ggttattttg tcgagctact tcaacgattt cttctaataa ttcttttgga
    27241 taaagtgccc cagtaggatt attaggatta atgataacaa tagcttttgt ccgactcgtt
    27301 atttttgatt tgatatcatc aatatcagga taccattctg cctcttcatc gcaaatataa
    27361 tgaacaggtg tgcctccagc taatgcaata gaagccgtcc acaacggata gtccggcatt
    27421 ggaaccagta cttcatctcc attgtccagc aacccctgca tacacatcgt gatcaattcg
    27481 ctcactccgt tgccggtgta gatatcattg atcgtcacat tcggaaaatg tttcagttgg
    27541 caatattgct caattgcttt tctagcagaa aagatcccct tggaatctga gtagccttct
    27601 gagtcacggg cattcatgat caaatcacga atgacttcgt ttggtgcttc aaatccaaaa
    27661 ggtgcgggat ttcccgtgtt caattttaaa atactaatgc cctcttcttg catccgctcc
    27721 gcttcttcta gaacgggacc gcgaacatcg taactgacac cttctaattt gttggatttt
    27781 tcaaattctc tcatcactta gccaatccct catttcaaaa agttagaact ttccaaattt
    27841 tttgttattt actatttcaa tatcatactc catcctagaa aattttgcca cttatttagg
    27901 aaaaacgtaa gtattaaaaa aacacaaaca agcgttgaga acttaattgt catgatagtc
    27961 attccataga ttcgctgccc aatccattgc aattccacaa atgattccta gcataaaacc
    28021 aagcaaaaaa cgtccgaatg caagtccaat caatacacct aagatcattc ccagtcctga
    28081 aaattctccg tcatatttct ccatcttaat aagtcctccc atttttgagc ttgacaacta
    28141 tgtaaaacct cgctatcatt agtgtgttat tattacgcag atgtgttgga attggcagac
    28201 aagcatgatt gagggtcatg tgagcgttag ctcgtgtggg ttcgagtccc atcatctgca
    28261 ctacaactcg tgaagttaat tttgcttcac gagttttttt gtttgaacgt ttctttatgc
    28321 cattatatac atatcactgt atcctttaca aaaagaacgt acaatcatgt aaacagcctg
    28381 aggtgagtat atgaaggtcg gaaacaagct actcgtagaa acttgcaagt gttaaaacaa
    28441 ttaatcgaaa aacgagtaaa atccctcacc taatactaaa gcgagggatt ttttgtatat
    28501 tatctgttta tcaccgttat tataaaggta caaataccaa gcacggttaa tccaaattgc
    28561 ataaattcat ttttttgtac actcatgctt ttcattagct ctttaactgc cattagtact
    28621 aagatggcga taacaacata aagtatatag tacgtccgca ttccggaagc ccatactgtg
    28681 ggagaaaatc ccataattaa ccttgaacaa aatcccatta tcatcaaaat aactaaaaaa
    28741 atagaagttt tcttattgtc aaaacttaaa taaattccaa caattaaaca tagtaataat
    28801 gccaagacta ctaaagtagc tatccaagta cctggataaa aaagtgacag tttcgttcct
    28861 aactctgtaa agagattatt caggttacta ctactccaaa tcattgccct tttatttcca
    28921 ttgacgtaag taaagctttg acccattgta ttaccaaaat aaataatcag gtttaagaaa
    28981 aacggtaatg cagtcaatat acgcacataa tagttttgac atttcctata tgttagaagg
    29041 aaaattaaga aaaatagcaa taaaaataag atattcatgt ctaaaaaaaa cggctttcca
    29101 aatgatgaaa tacctaaatc taacttatta agaaagttga aatttttata ttcaggaaac
    29161 catttattaa tttcatgtgc agcacgtaat gcattccctg gagttgtcaa agagaaaatt
    29221 aattctgcta agcttattat tgaaaatact gataacttat aattatatcg tccattgaca
    29281 atcaaatata aactaactat acttgttaga acaaaaaaac aggcgttgac ttgttcttga
    29341 ttagttaaaa atatcaataa aggaatactt acccaatata tttttctatt tatttcttct
    29401 cctcttaaga gttgaaaaaa cggataaaaa gttagtaaac taaatgctac tggccactga
    29461 tagttcaagg tggttgctct ccaaccggtt tcccccatta ctgtcaatgg tattaaacag
    29521 tatatagagc aaaccaataa taaattttta acatttattt tctcaaaaac ata
//
LOCUS       DACKAC010000032        29443 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.032, whole genome shotgun sequence.
ACCESSION   DACKAC010000032 DACKAC010000000
VERSION     DACKAC010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.032"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            553..1086
                     /locus_tag="I0B46_001457"
     CDS             553..1086
                     /locus_tag="I0B46_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAR2083766.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(1576..2100)
                     /locus_tag="I0B46_001458"
     CDS             complement(1576..2100)
                     /locus_tag="I0B46_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAR2083767.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(2093..2566)
                     /gene="tsaE"
                     /locus_tag="I0B46_001459"
     CDS             complement(2093..2566)
                     /gene="tsaE"
                     /locus_tag="I0B46_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAR2083768.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(2689..3669)
                     /gene="pta"
                     /locus_tag="I0B46_001460"
     CDS             complement(2689..3669)
                     /gene="pta"
                     /locus_tag="I0B46_001460"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAR2083769.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(3822..4502)
                     /locus_tag="I0B46_001461"
     CDS             complement(3822..4502)
                     /locus_tag="I0B46_001461"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAR2083770.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            4628..5497
                     /locus_tag="I0B46_001462"
     CDS             4628..5497
                     /locus_tag="I0B46_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAR2083771.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            5523..6038
                     /locus_tag="I0B46_001463"
     CDS             5523..6038
                     /locus_tag="I0B46_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083772.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(6085..6648)
                     /locus_tag="I0B46_001464"
     CDS             complement(6085..6648)
                     /locus_tag="I0B46_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAR2083773.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(6703..6882)
                     /locus_tag="I0B46_001465"
     CDS             complement(6703..6882)
                     /locus_tag="I0B46_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083774.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(6959..7468)
                     /locus_tag="I0B46_001466"
     CDS             complement(6959..7468)
                     /locus_tag="I0B46_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083775.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(7564..7944)
                     /locus_tag="I0B46_001467"
     CDS             complement(7564..7944)
                     /locus_tag="I0B46_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAR2083776.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            8274..9023
                     /locus_tag="I0B46_001468"
     CDS             8274..9023
                     /locus_tag="I0B46_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAR2083777.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(9207..10484)
                     /locus_tag="I0B46_001469"
     CDS             complement(9207..10484)
                     /locus_tag="I0B46_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083778.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(10563..11207)
                     /gene="pcp"
                     /locus_tag="I0B46_001470"
     CDS             complement(10563..11207)
                     /gene="pcp"
                     /locus_tag="I0B46_001470"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAR2083779.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVSFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(11406..12350)
                     /locus_tag="I0B46_001471"
     CDS             complement(11406..12350)
                     /locus_tag="I0B46_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAR2083780.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(12352..13038)
                     /locus_tag="I0B46_001472"
     CDS             complement(12352..13038)
                     /locus_tag="I0B46_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAR2083781.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            13512..13832
                     /locus_tag="I0B46_001473"
     CDS             13512..13832
                     /locus_tag="I0B46_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAR2083782.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            13986..14891
                     /locus_tag="I0B46_001474"
     CDS             13986..14891
                     /locus_tag="I0B46_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAR2083783.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            14888..15661
                     /locus_tag="I0B46_001475"
     CDS             14888..15661
                     /locus_tag="I0B46_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAR2083784.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(15842..17266)
                     /locus_tag="I0B46_001476"
     CDS             complement(15842..17266)
                     /locus_tag="I0B46_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAR2083785.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            17477..17923
                     /gene="nrdI"
                     /locus_tag="I0B46_001477"
     CDS             17477..17923
                     /gene="nrdI"
                     /locus_tag="I0B46_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAR2083786.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(17988..19970)
                     /locus_tag="I0B46_001478"
     CDS             complement(17988..19970)
                     /locus_tag="I0B46_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAR2083787.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(19984..20880)
                     /gene="murQ"
                     /locus_tag="I0B46_001479"
     CDS             complement(19984..20880)
                     /gene="murQ"
                     /locus_tag="I0B46_001479"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAR2083788.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(20883..21944)
                     /locus_tag="I0B46_001480"
     CDS             complement(20883..21944)
                     /locus_tag="I0B46_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAR2083789.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(22155..22682)
                     /locus_tag="I0B46_001481"
     CDS             complement(22155..22682)
                     /locus_tag="I0B46_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAR2083790.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(22749..24251)
                     /locus_tag="I0B46_001482"
     CDS             complement(22749..24251)
                     /locus_tag="I0B46_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAR2083791.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(24509..25630)
                     /locus_tag="I0B46_001483"
     CDS             complement(24509..25630)
                     /locus_tag="I0B46_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAR2083792.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRKNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            25756..26697
                     /locus_tag="I0B46_001484"
     CDS             25756..26697
                     /locus_tag="I0B46_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAR2083793.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(26847..27743)
                     /locus_tag="I0B46_001485"
     CDS             complement(26847..27743)
                     /locus_tag="I0B46_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAR2083794.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            28012..29352
                     /locus_tag="I0B46_001486"
     CDS             28012..29352
                     /locus_tag="I0B46_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAR2083795.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
BASE COUNT         8483 a         6108 c         5274 g         9578 t
ORIGIN      
        1 ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt ttattaagag
       61 gttgtgaaaa agctgcactg catcaagtaa taagaacagc caaacaaaaa tagttcctca
      121 tatttttcgt ttggttggac ttattaccgc agatgcaagc ttttgaacac cgtttattga
      181 gaagttgtga aatcagcgtc ctgacctgag tattagagaa acaaatggca aaatagttcc
      241 tcatattttg ctattttttg agctaatacc gagggtcagc tgattgaaca ccgtttagtg
      301 gagaagttgt gaaaaagtcg cctactttcg agtcgtaaga aatttttttg aaaatggttc
      361 ctcacaactt tgcataaatt tgacttaata ccgagaagtc atgatcccca tctaactcca
      421 aatcacaagg aatgagttca aaaactagct tttctcaaca aatattacat aaaaaattct
      481 atgttttttg ttaaatttat ttgctttttt caaaagtatg ttacagtaat ttcccaagga
      541 ggttttacag aaatgaattt tgttgccatg gattttgaaa ctgccaatca tcagccttat
      601 agtgcttgtt ctcttgcgct agtcatggta aaaaatagcc agatcgttga tgaattctat
      661 acattgatac agcctgagac tccttttttc tggcgaaatg tccaaattca cggaatccat
      721 caagaagacg tacgcaatgc gccgaaattc ccagaagtct ggcaaactat ccaaccatat
      781 tttcaaaaaa atcgtttagt cgttgcacat aatgctgctt ttgatacaaa ggtcttagcg
      841 ggatgcttag attattatca tcttgaacag cctaattatc tttctttatg tacagtgaaa
      901 agtagccgtc gcctgtttcc tgaatttcca aatcatcgat tgaacactgt gtgcgaaaat
      961 ttgaacatcc aattagatca tcaccatgat gcattggaag atagtcgtgc atgtgcggaa
     1021 atcctgcttt atcaagaaaa aaaattcggg acagatccat tgaaaaaact tgtgactata
     1081 aagtaaaaga atacttatga aacacacaaa gaggtcgtga gcctgccgtt tcactccgag
     1141 tcacaaggag caatttcaaa aaatagcttc tcatgtttat taaatattgt gactggtgac
     1201 cgaggagctg gcaggagaac accgtttatt ggagaggttg tgaaaaagct gttctgcatc
     1261 aagtaataag aacagccaaa caaaaatagc ttctcatatt tttcatttgg ttggacttat
     1321 taccgcagat gcaagctttt ggacaccgtt taccaagagg ttgtgaaatc agcgttttga
     1381 cctgagtatt agagaaacaa acggcaaaat agttcctcat attttgccgt tttttgagct
     1441 aataccgaag gtcagctgat tgaacaccgt ttattcaaaa aaaatggact gcgacacgac
     1501 ttttgtcaca gtcccttcgt gtgtttttct gccttctcaa aaggatgaca gtttctcact
     1561 cttttcctat ttcttctatt caatcaaata actcatttta tatacatcta aaaattcacc
     1621 tagatctgtt cttgcccctc taggcatgac cgcttcgatt tggaagccca tttttcggta
     1681 aaggtgtacg gctctttcat tacgaacttg aacgtcaagc tccaatcgaa aaagtacacc
     1741 catttccttc gcccaactga tgatttcttc tagcataaga gtgcctagtc ccattcccca
     1801 atattcctgc agaatactga taccgacttc tcccacgtga gacaaacgaa attgactgtc
     1861 tgccttgaca gatgctgttc caataatcgt cccttcatag attgcaagca gcaataaatt
     1921 attattgctt tcgtatatat aatccaactc ttcttctaag gtttctggtg gtaacaggag
     1981 ttctgcttca tccaaaacta aaaattctgt ctccttatta acttgtccca tcatcaaaag
     2041 aatgtctttt gcatctcctg gcactgcttc ccgcaagatg atcccaactt cttcactcat
     2101 gattttcagc ccatttttct agaattcgct gtttgaaggt ttcactttgc tccccgtatg
     2161 agattagttt cacataccgc ttttctaaat ctgtatcact tttttctaaa atcagttcca
     2221 aataatcctc cggcagtgct tcttctaata gatttcccca ttcaacaacg gaaagcccat
     2281 caccttcaaa atattcatct aaccctaaat ctgctccgct agctgctacc cgataaatat
     2341 ccatatgata caacggaatc cgaccttgac tatattctct aataatcgtg tatgtcggac
     2401 ttttgatcat ttgcgttatc cctaatcctt gggcaattcc tttcgtcaac gttgttttac
     2461 ccgcacctag atcaccagtc aatatcagat tgtcacctgg cattgcggaa tgaccaattg
     2521 cctctgcgaa ttgttctgtc attgttaatc ctgatagttc gatcattttt catcttctcc
     2581 cgctcttctt tctttttgcc agtatatcgc gtaatgaagt ttttttaaag aaaaaatgaa
     2641 ctagtcaaga acagcagctg ctgtttttgg ctagttcgcg tgtatttgtt aatcaatcaa
     2701 tgtttgtgct gctgtgataa tcgataattt atatacatct tcttcatttg ctccacgaga
     2761 caaatcagaa accggtttgt tcaagccttg tagaattggt ccgattgctt caaagttacc
     2821 gaaacgctga gcaattttat agccgatatt tcctgactgc aattctggaa atacaaagac
     2881 tgttgcttga ccagctactt ctgaatctgg cgctttcagt tgtgcaactg aaggaacata
     2941 agaagcatca aattgcaatt cgccgtcgat catgtattgt ggcgctagtt ttttagcgat
     3001 tttagttgct tcaactactt tgtctacttc tggcgatttc gctgagcctt ttgtcgagaa
     3061 gctcatcatt gctacttttg gttcgatatc gaacaattct gctgtttttg cagaatctac
     3121 ggcgatttca gccaattctt gcgcagttgg attcacgttg atcgcacaat ccgaaaagac
     3181 atatttttct tgatctcttc cacgtaccat gatcatcgct ccgcttgtcc gactgatgcc
     3241 aggctttgtt ttgatgatct gaagtgctgg gcgaacagtg tcgccagtcg aatggatcgc
     3301 tccagagacc attccgtctg caatccccat atacgtaagc atcgtaccaa agtaattcac
     3361 atcttttaaa atcttttctg cttgttcttt ggatgccttt ccatttctac gttcaacaaa
     3421 agtatccacc atttcatccc acttatcgta attatcaggg ctgatgatcg taaagctaga
     3481 tgttttgatt cctctggcat gcgctgcatt tcttatttct tcttcgttcc ctactaaaat
     3541 gggttccaca agttcttctg ctttcaaccg ggcagctgcg cctaaaatac gagcatcagt
     3601 agcttccggg aaaacgattt tgatcccacg gcgaacaatt ttaaatctta aactatcaaa
     3661 caattccaca agatgacctc cagacgtttt tatatgtatt atcatacact attccagaaa
     3721 aaaagaacag tttattttat ctgtatcagt caaaaatgct ggaaaaatta agtttttcac
     3781 atacaaagcg aactgcacta taacaggaaa gcgctgtttt attacactgt ttcaggcagt
     3841 tgccaatcaa tcggttcttc tcctaacgca aggagcgcat cattcgtttt cgagaagggt
     3901 ttggaaccaa aaaagccacg acttgcagat aacggactgg gatgtggaga tgttatgatg
     3961 atatggcgac tcttatcgat cattttgatt ttttcttgtg ccggttttcc ccacaagata
     4021 aacacgattg gtttttcccg ttcatttaat ttttcaatga tcgtgtctgt taatctttcc
     4081 caacctttcc cgcgatgaga atacgcttgc ccttcccgaa cggtaagaac ggtgttgagt
     4141 aaaaggactc cctgtttagc ccatgacact agatttccat gttgaacagg tgcgattcca
     4201 agatcatcat aaagctcttt atagatattt cttaacgatg gcgggatctt tacgccaggt
     4261 tgcacagaaa aagataagcc atgagcttga ttttctccat gataaggatc ttgtcctaaa
     4321 atcaccactt taacttcttc ataaggagtc aattccaatg cttcatagat atgatacata
     4381 tctggatgga tcttttggga cgcatattct ttttttaaaa attctcttag atgcagataa
     4441 taatcttttg aaaattctga ggacaaaact tcttgccaac tgttatgaat gatttctttc
     4501 aaaaactcca cctcctaata aaaagaataa ctcacatttt cttcaatcac aagggacaat
     4561 cttcaccctc agcacttgat taatatggta aactaggaac aagaagcaga aaggaacgat
     4621 aatcattatg atcaaattaa ttgcatcaga tatggacggc acattattag atgcccatat
     4681 gagtgtttct cctgaaaatg ccgaggcgat acgttttgca aatgatgccg gtgttgagtt
     4741 catggtcgct actggtcgaa actatcaaga agctcgcgca gctttagatg aggtaggaat
     4801 cgattgtgcg atgatcactt tgaacggggc acaggtattt gataaagaag gaaattcatt
     4861 gtttaccgtg tcgattccaa ataatcaagc aatctctgtt ttagatattc tagatgctaa
     4921 cggtatttac tatgaagtag caaccaacga tggtctttat tctgaaaatc aagcgaaacg
     4981 aattgaaagt tttgcttcca tggtggctac ccatttacct caccttacgt ataaaatggc
     5041 gattgccatg gcctcagcta agctggagtt attgcacatc acatacgtgg acagtattcg
     5101 tgctattttg gaagaaaaga atctggaagt tttaaaaatt atttgttttc atacagaagg
     5161 gccagctgta ttaggtccag ttggaaagca gatttctctt ttaggcgatc tagccgttac
     5221 ttcctctggt caaaacaata tcgaagtgaa ccataaaaac gctcaaaaag gaattgctgt
     5281 tgcccatgtc gctcacgagc gtgggatttc tttagaagaa gtcatgacga ttggcgataa
     5341 cttcaatgat gtcagcatgc tgcaagtagc aggagtcagt ttcgcaatgg gaaatgcaga
     5401 aatcgaagta aaagattatg ccaaatatac aacggacaca aaccttgaat ctggcgtagg
     5461 aaaagccata ttgcgcgcga ttgcagaaga cttgtagttt tatctagaaa agagggatat
     5521 agatgtcaga atttttcatt caggaaaagc agcttggacg tgcaactcgg acaattgtca
     5581 aaaacgaaaa aggacattcc ctgtttttga tggttggtcg ctggggaaca aagggcgatg
     5641 cattgtctct ttatgccatg aatggcgatc tcgttgctca catcaagcag ataagcctga
     5701 cattcggtac gcgttttgag ctgtataaag agttcgaaaa ggtgggaacc atgcagaaaa
     5761 tattcaattg gcccggtgac ttttattata ttcgtagact tcactggaca gtccaaggtg
     5821 atatctataa tcatcaatat caaatccatc atttcaatca gcaaatcatg aaaatggata
     5881 aagcaacact tttttcagga gattactatg tattggatgt ctcaaatgat gaagatgctc
     5941 cagtttgtat ttgtattgct gcggttttag attactggct ctaccgtaaa gacaaaagtc
     6001 gcgataagcc tgtttcttac cgacttaact taagttaaaa agatgtacct gaaatgcgga
     6061 aatacgcatt tcaggtacat ctttttagat attcttttgc atatgatcat aaagatgacc
     6121 actgatcatc cgctgaccga caactttgaa gtcatggcgt tcgtataatt ttttagcctt
     6181 tggatttttt tcatccacac ttagaccgat gacggagcga ttagctttct tcgccaattt
     6241 aggtaatgct tctaataatc gcgaaccgat gccttgtcct ctaaaatctt ctgatacact
     6301 gatagagtcc aaataccatt cgttgggaaa cgtttcttta tcaatgaaca aacgtgtaga
     6361 cgcatctagc tgatatttct ctaagatagg taccagtggc tgatcgatga ttgattcttc
     6421 ttcatctgta taaccgtaag caactcctgc aactttaccg tctatttctt ctacgatacc
     6481 tcgtgaataa ctgtaacgat aatttggatc agtgattgct tcttctaaaa tctctaaagt
     6541 cttttgttcc cctgtctgct tcaaaaatgg cagttccata tcttttagaa tcactaagat
     6601 caatggggcg atttctggag catcttcttt tctcgcaaaa cgaatcatag tttcctctcc
     6661 ttttctccat tctaacatgc agaaaaagaa atcgcaaaat cgttatttta aaaaatcttc
     6721 aatcttttca gtaatgaaat aaccatgagt aacgattctt gccgcttcag ttcggcaata
     6781 agggcagacg actggcattt ctttatcatt atgttcgact acattattga cttcttcgta
     6841 tgcaaatttt tttccgcaat tagggcattt cattttttcc atgttatctc aacttccttt
     6901 catctttact ctcttagctt actcttatat tgtaaaaaag agaagcaata tccttcttct
     6961 atgcagatat ccatacctga cggtcctgtt ctccatctat taatcggact ttataataag
     7021 gacgttcatc tttgactaga agtgtccact ctttcacttt tgcttttttc ccgtagtagc
     7081 tgactgcttc cttcatcgct gcttgcggtt gtttgaactc ttccgttttt tctatatcaa
     7141 aaagatgttc tgcggccttt tccgtgtcat ttcgtacggt tgttttccca gtggatggat
     7201 tgactgctat gacttgtcct tcacaaaaaa actggtattc atatgttgca ttcgtaggat
     7261 tctccaaatc tttttcaact gcaaaatgaa tagcagatat gttatgttct tttgtttcgt
     7321 tttttacgat tgctgctgct tttttcaatg agatccgata ttcctggtca ctaatatcat
     7381 ccatgtgaaa agcggcattg agcagtcccg tcataccggt aatccctatt acgatgaggg
     7441 cagcaacacc aaacatcatt tttttcattc ttcattctcc tgtttctttt ttttaaagaa
     7501 tatcaagaaa tgaacgaatg aaaaatcgaa tgaaacgaaa aaaaagtgac aattttgtta
     7561 tctttattcg tgcagttcta atggcaatcc gtctggatca aagaaaaacg tcattttttt
     7621 tcctgtaaag gtatcttctc taatcggttc tgattcgatc cctaaggact gcaattctgc
     7681 aattgtttct tcaatatcgt ctactttgaa agcaagatga cgcaagccac aagcttctgg
     7741 atagtttggt cgttgtggcg cattagcaat tccaaaaatc tccaattcag cacttcctag
     7801 tttcagatct aatttatagt catctcgttc tttgcggtaa ttttctctga ttacttcaaa
     7861 tcctaattta ttaacataaa aatcacgtga tttctgataa tctgaaacaa tgatggctat
     7921 atgatggatc gttgtaaggt tcatatgagc actcctattc ttcgtttaag ctcggatcta
     7981 tattaatctc cagttccatt gtaaatgtgt cagattgaaa aaaatagctg tttttccttt
     8041 ttataagaaa aaaggcaaaa aaaacagaaa gtaggtcctc caagacactc ttcctgttcc
     8101 caatataata aatgaaagta agaaattata ttttcaagaa aacaaacaga aaattatcat
     8161 aacacaaatt atttataatt gaaagactag ttttttttca agaaaagcat acaatagatt
     8221 gtgttagtaa atacagaaca cactacattt agtgtcttaa ggagtcctca tacatgaagt
     8281 catattttca gtttttagct catcagctaa aaggctggcc gcaacaaaat tattatcttt
     8341 tctttttcag tcttggctgc caggtcatga ctcttgtcaa tagtcccatc acttggctgt
     8401 ccgttattac atttatcggt accacgcttg gtgtattgtg tattttatcc atcaatgcgg
     8461 caaaatcagt caacggtatt ttaggtattt tgtctgcgat ttgttttatt atcgttgggt
     8521 tttcagcaaa gaactattta agtatcggtg aacagattgc ttatatgatc acattggata
     8581 tccccgtatt gttaagtaaa gaatggaatc ataatatggc ttcaaaaatc cgaaaattca
     8641 ctggaaaaag ctggtttatc gcaatcatca gtactttcct tgtctatctt gtttcaggat
     8701 tcctgattgg tcgttataca gatgatcctc ggccgtgggt agatgccatt gctttttcta
     8761 ttagcttgac tgctggtatc atcagtttct tgcgttacaa caaccaatat tactggtggt
     8821 tagcttcagg gcttgctcaa atggtccttt ggtatatttc atttacgcat ggatctgcaa
     8881 gtttggcgat gttcgtgaac agctctgttt acttgctgaa tgatgtcctt gcatttaccg
     8941 tttcaccttg gtataaccaa aaagaacgtg aacgtatgca aaaaatggaa gaagaatact
     9001 acaacgcaca acaacacgca taaaaaaact gagcgtggga caaaagtaaa gaacactttt
     9061 gtctcacgct ctaaacacgt ataaacggcg gaatcagaag caactccttc ggaaataagc
     9121 tgaaattcac aaaaatttga aaagcaattt tcgtgaattt cttcttattt cttggagtta
     9181 aacgcttctg tcccagcctc tattttttat agtaattcct tcagatactt tcttgcctca
     9241 tcattgaaac gcagtgattc ttctaacttc tgataatatt ccaacgcttg tttctgagct
     9301 tcagtaaaac gataatgttt tcctttccat ttgtcgattg tctcttgcac atttccagat
     9361 tggatatctt tcactactag ttgccatttt tcttttgaga ctgtacggtc tttcttatca
     9421 gcagattgta tagcttctcg ttctttacgc aatactttta aaaaaccatt tctgactatc
     9481 cgaggaatgc tgtcgaagaa cccttctgta aaaccatagc gacaatattg cttccaaata
     9541 aatagataga tttttggttg gatgaatcgt tggtttttcg caaaatattc catctgagca
     9601 gtgacttctg gtgttcgttg acgttttgtc tttctcaaca cggaaataaa tgtttcaaaa
     9661 taagcattag gatgtactgg cacttcttca tatacgatgg attgccaatc tttacgtaaa
     9721 aatgcgcgat agttgatcaa gaattcttct acatccttta ctaattcttc tgcaaagtcg
     9781 tcaaatagtt ctttttttat ctgtcgcttt tttgactgcc cattgatgtg ttcacttatt
     9841 ttcttgattt cattttccaa tgcttgataa tccgctatgt aaatcggcat ttctacttgg
     9901 cgaaattctg cgagccgctg agctaatttt atatccggta aatatggctg tttcatccga
     9961 cgattttggt acaagacaaa acagtatttt tgccatagtc cttcaggaaa atcgatattc
    10021 ttctgcttaa gccataataa ttcgtctaag gccagatcaa cttttgtcat tccctgatgg
    10081 atcgaagctg ccactgtcgg agagacatgc aggtgatcgg caaagtggtt gatgcctagt
    10141 tttaggatct ttccggtttt tggcgattta acgatgtatt gcacttttaa ttctctacca
    10201 cactcacaat acaatttttg ctggcttcgt tttgttcgaa aaaacggatt gatcttgaag
    10261 tcagaaaaca cccaatctgt tgcagctaaa taatgttctt ccataaaacg agagctgatt
    10321 agatatcggc ggtgcttatc gactagttcc cgctgttttt tcgttaattt tgcatagatc
    10381 tcccaacgtt gactgcttgt gtattgttta gaaattttga tgggatagct tttgctgatt
    10441 tcttttggaa accattcttc aaagacggat tgccattctt ccacggctgt ttctttcctt
    10501 tctaattaca aaaagctggc tactaacata acgttagcaa ccagccagtg tttcttgcta
    10561 aattagtgag ttgttccggc atttccggaa atatcttctt tattttctac tgctgcttca
    10621 actgcataag tcaaagaaga agcaatgtct tctaaagaca tagaaggcgt acccactgga
    10681 cgatcgatca cttgatcggg tgagaatgac acgtggatga acccacctcg aattgccggg
    10741 aattgtgttt cgatcaaatg aagcaaacga tacatgatat cattgcagac aaatgttcct
    10801 gctgtataag agatgtacgc tggtagacca tgatcttgta cattttttgt cattgctttg
    10861 attggcaatg aggtaaagta agcggctgga ccgttttctt ctaaagccgt atcgaatggc
    10921 tgatttcctt cattatcagg gattcttgct tctgctagat tgattgcgac acgttcgaac
    10981 gagactgctg accgcccacc tgcttgtccg acacagatca cgacatctgg ttggtgctgt
    11041 tggatggctt ctttgactac ttgactgctt ttttcgaata cggtcggtac ttccactttg
    11101 atgatttcag caccgctgat ttcatctggc atttttttta ctgcttcata ggctggattc
    11161 actttgtccc caccaaatgg atcaaaacct gttactagta ctttcatctg ttgttcctcc
    11221 gtccgattga tttaacatat ggttatcttt tatcatacca ttttccttga acagcgtaaa
    11281 taaaggaaac aatagtttaa ataacataac agatcaaact taatatattc ttataaaaaa
    11341 atagtgttcc actacaatgt tagaagaggt tgcgacaatt attctgtcac aacccctctc
    11401 ttctattatt taaatagaat catataagca atttggaaaa ctagcataaa taacgcaata
    11461 aacaactggt ttttgatcac tccgtatttg tctttcatct ccagcatcgc tacaggcacg
    11521 atattgaagt tagcagccat cggtgtcagc agggtcccac aaaaaccgca agtcaatgca
    11581 accattccta ttaacgctgg attcgctcca tgactaaata cgaatggtgc accgatccca
    11641 accgtcatca cggtgatggc cgcaaatgca ttccccatga ttaccgtaaa tatcaccatg
    11701 ccaagagcgt aaatgatgat gccgatgttc acatttcctt ctggaacgat cgtacctact
    11761 gcattggaaa tcactgtccc aactccagcg cttgtaaata ccgcacctag agaagctaaa
    11821 agcattggca acatgcttaa cggccctact gtgcccagca tatctgctgc atcatctaaa
    11881 aatgtacttg gtcgattatc tcgagaaaag aacatcaaga ttagcatggc tgcaaaaaca
    11941 ccgacgccca cgccgactaa agcacctaaa ttggtaaata gagcaaacaa gatcgcaaag
    12001 acaccgatac tcaaagctgg aataaaaatt ttcattccta gtttgtctga cattttttcc
    12061 atatattctt ttgatggaag ccggctttct cctttgctga ctttcttagc aattgctggt
    12121 atggtcatcg caaagatcaa gccaccgttc acgattgccg gaagccatct tcctcctgca
    12181 ataactaccc ctaagaccgt ccagaaaaag gctgacccat aacgatgagg attggtttta
    12241 tctgttaaat ttcttactcc agtgtaaatc aatacgagtc ccatcacgat ataaaggatc
    12301 tccagtaatt tatcgcctaa taaaatatca ggatttgtaa aaaagtccat cttattgccc
    12361 ctttcctgga taatatttct tcgctaattt cttttcttta agatagaagt agacacttgc
    12421 cacaactaaa gccgtgatag ccactggaac ttccactaaa gcaagctgtc ccaatgttac
    12481 gtcatatcct agatctttca gtgttgattg gactagcaac gcccctgctc ctccgatgaa
    12541 tagtacttgg ccaaagaacc agcagacatt ttccatagct gaagccatac cttttagttc
    12601 ttcttcatgc ttaggatgga cttctaaccc ttttgattcg atggtcccca atgccatcgg
    12661 taaaatgatt ggacgaacaa atccagcaac tccaccaaaa ctcacgttaa aagccgcaaa
    12721 gatcattcga aagatcccat aaatatctac aatcatacca gcagatactt tcttgaatcg
    12781 cttgatcaag gaagccgctg attctttcag accatttctt tcaagcgttc ctgttgccaa
    12841 cataatgatg ataaaaatcg ccatcccgcg attgtttaca aaacttgtac ctaaaagttc
    12901 taacattcct tgaatgccaa gaccgccagt caaagctgta acaactaaag caaccattac
    12961 gatcaatatc gagtctaatt taaatgcaaa cccgagaata acaatcaaga cgcctaaaag
    13021 gctaagataa ttttccatat attttcctcc ataaaatgac aatatttttt taattataaa
    13081 gcaaaacaca taaaaaatat agttgttttc tcacgaaata gacactattt ttaaatattt
    13141 ttcgttattt ttttatcaca aaaattatct tacttattgt aaggtaaagc tttttttaat
    13201 ttattgcccg ataatatata gaaataaggt ctagttactg ttttttttaa atgattaata
    13261 aataacaact tctttctatg aaattgcaaa gcattcatct tcttttccgc taacgatata
    13321 tccgtcagtg cttggtacac tttactaaaa agtatgagat tgccgatatc tggataggtg
    13381 tgctatacta cagaaaaaga tatacaatct gccaccgggt aggataaaga ggatgatcat
    13441 tccattaggt ctttgaaggg atgattttcc tctttttgtg tattttttta tagttaagga
    13501 ggaatgtaag tatggcatgg atggaattaa tcatagcagg aattttggaa gtattctggt
    13561 caacgatgat gaaatggagt gatggttttt ctaaaatcaa ttattctttt tacacagtga
    13621 ttggcatgat tgctagtttt tactttttgt caaaggcaat caaaagtttg ccgatgagtc
    13681 tctcttatcc tatctggaca gggattggcg cagttggttc ggtcataatt ggtgtggtct
    13741 tgtttcatga taagctgaat ctttccactt ggttctttgt ggggatgcta ctcatcagta
    13801 tcattggcat caagattact agcggccact agacacttgg aataagtact gatcagaaaa
    13861 aataagcctt tttatttaat attcaatttt tttatgaaaa aataagaaat aagtccaatg
    13921 acctagcgac ttctccactc atcaggtgta tgcttgtact atctatagaa aaggagtgaa
    13981 tatttttgaa aagtctttcg gtccaagaat tatccaagaa ctttggtcac aaacaagccc
    14041 tcagcgatgt cagttttagc ttatcccctg gcaaaatcgt tggattagta ggtcctaatg
    14101 gcgctggaaa aacaacgatc atgaaagcta ttttaggact gatccattac tcagatggaa
    14161 caatctgtat tggtgaaaat gagatttctc cctctagtca taaagggttg gaacaagtag
    14221 gagcattgat cgaatacccg ggtatctatc cattcttaac aggctatgat catctgaagt
    14281 tattctctga aaccaacgat gtatctgcta tcgacactat cgtgaatcag ctcaagatgg
    14341 aaaagtacat tcacaaaaaa gcgaaatcct actcgttggg aatgaaacaa aaattgggaa
    14401 ttgctcttgc cttactgaac catccgtcta tcgttatttt agatgagccg atgaacggtt
    14461 tggatccaca agcaacaaga gatgtgcgaa aaatcattac cgacttggct actcagggca
    14521 cctctttctt gatctccagt cacttactca gcgagcttga aaaaatggtc gatgagctga
    14581 tcctgatcaa tcaaggaaag atcatcaaac aatgtacaat ggctgaattg aaccaaacag
    14641 cgcaagattt catcgttatc tctacaacaa atgatctagc tgctcgaacg atcttgacag
    14701 aagctggata tactttggaa gatcctgata aaatcaagct tttaaaagaa aacgaagaat
    14761 tattaggaac cgtgatccag ctattgtctg cacaaaatat ccatgtgaca gacgtccagc
    14821 atctgacgaa tgatttggaa tcttctctat tggaaattct ggattcagta aagggggaag
    14881 aagaaaaatg acattgcttg taaaacaaga attatttaaa ttgatcaaga aaaagtcaac
    14941 tgctgtttta tctgttctat tagttgtttt gctcatagga acagcacttc tagccaaaaa
    15001 atacacgacg atcattgatc cagttgaaat gactgctcag ctttttagtg ccacatcttg
    15061 gattgtcttt atcatgattg cagctgcaag cacgatcatt tcgatggaag cacaatatgg
    15121 tacattgaaa aatctccttt atcgaaagta ttctcgagga gaaatattag taagcaaatg
    15181 gatcacatta gttatttatt ctgtttattt atacctttta gctattattg tcactgtgtt
    15241 gatgaaactg attcttttcc cttcgatttc cttcacggag aaggtttcta ctggtcaaac
    15301 actgatccaa agcttgttca cttatacttt gggtagttat atcggtttat ggctgatttt
    15361 aagtttagtg ttgatgattg cttgctttat caatagttcc ggcgcatcca tctcagcagg
    15421 aatcgtcttt tattttgctt cgtcaatcat atctggtatc cttattgcac tcatccaaaa
    15481 atgggagtgg atcaaatgga atccgatcag tatgttgaat ctgcaaaacc aaattggaaa
    15541 cgaagagatc atgaagcaat tgactcatct ttctactaat caaatgctat tcggtaattt
    15601 agcttacatc gttttgttct tagcattagg atatctcgta tttaagcgaa aaaatgtatg
    15661 atgactcatc aaaaaagagg ctgtgaaaga gacgttcagc cttattgccg attggctgtc
    15721 tcttaaacac cgtttattct gaaaaaggag ctgtgacaac atttgtgtca cagctccttt
    15781 cctagtatgt ttttgaataa acgacaggct cacaacctct ctttatactt cttttatact
    15841 tctatacgtg aagcatccca gtggcaaata aaaccattgc aatcactccg accatcgtca
    15901 cacctataag taaaatctta tctaagtgga tatgttgtac aggctcgttt tgctccttac
    15961 gtgctttgag gtaaaaataa atgcctggca agaaaagaac aagaacaagc attacttcat
    16021 taaaggctac gactagcata gcccatactt gaaaggcact tgctaaaagc cctactaaaa
    16081 attggaccca ttctttccgc tgataagaaa gtttcatttg gtacaatcct aagaaaagcc
    16141 acgtgaagaa aatagctgct gtaccaagtg tgtaagcaaa gttataagca ctgtcagtga
    16201 ataataatga aagtaaaaag acattcgtac ataacgtcgt caatacgata gcataagttg
    16261 gtgccccatt tttgttgagt tttccccatg ctttaggcaa aagtccgtct tcagccatgt
    16321 ttcccattgt ttcaatcggt aagatcgtcc atgagagcca aacaccaact aaagaaatga
    16381 tcaaaccgcc gttgatcacg actgctcccc atttaccaac catcatttca aacacatagc
    16441 ccatagcagg ctgcgccttg atcgtttgca gctgattcat cgtcattgtc ccatatggaa
    16501 gcatggaaac caagacgtaa ataataatca agccgaatat ccctagaatc gttgctttcc
    16561 caacatctga ttgttttttt gctcgtgaac ctacgacaga ggctccttca ataccaacaa
    16621 atacccaaag catcaccatg atgcaactca atacttgact ggaaaacggg atcggctgtc
    16681 cattcgcatt caatataatg tcgctagaaa agttatcaat gaattgttgg atcgaaaacg
    16741 tgctgatagc tactacaata aataaaaata atggcactaa tttacagaat gtgacgatag
    16801 cattgacgaa actagcattt tcgactcccc gattcactag ccatgcatat ccccataaaa
    16861 agactgtact catcacgatc gatgccagat tttgcccacc ttcaaatact gggaagaaat
    16921 atcctaatgc gctcatcaac aatgttgcaa aagctacgtt gcctaaccag catgtgagcc
    16981 agtaacccca accgctgata aaaccagcga aacggccaaa accttcttct gcgtaactaa
    17041 agatcccgcc ttttaaatct ggacgttttt tattgatatt attgatcgac aaggagagca
    17101 tcaacattgc tgttcctacg agtacccaac tgaaaatagc tgggccgcca gccgatccgg
    17161 agatgtccgt cataatacca aatacgccac ctccaatcgc tgaaccaatt actaaggcag
    17221 caagtggaat taaaccaatt ccttttttct gttgctctgt atccatgtaa gcatcctcca
    17281 aaataaaacc gcttttatca aaaagtataa caaacctttt ttatttttac ttaattaata
    17341 aaaactttaa tagaaatata attctaccgg cttttttctc attatacaag caaagcgcct
    17401 agtccctttc caaattacac caccaaaaaa cttaaaaaag atgctatact aaaaggaaag
    17461 aattggagga tgatccatga acattttata tatatcgatt tcaggaaata cgcgtgcctt
    17521 tgccaaacat ctagcagaat atgctgaaaa aatgcatgca gaagatcctg tcaatcctga
    17581 agttacttta aaagaaatcc atgaaaatag tgattttacg aaagaaacag aaccattttt
    17641 tactttcgtt cctacttatt tagacggcgg aaatggttta gataacggtg atacagaaat
    17701 cttgaccgaa acgatgcgtg aatatctgga acatgaagac aatcataaac tttgtcttgg
    17761 tgtagtcggt agcggaaata aaaacttcaa taatcagtat tgtctaacag ccaaacaata
    17821 tgcacaaaca tttggctttc ctttcttggc agattatgaa ttacggggaa caccatctga
    17881 tgtgacacga atttatcaga ttttagcaga aaacaataaa taataaaaaa gaagaagctg
    17941 aaaaatagat ttcagtttct tctttcgttt ctattgtttc tatcatatta agctttgaca
    18001 tccatcacag gtgcttttgc ttttgcttta cctgtttttt ccaagacaaa gttttttaca
    18061 cgatccatat tggttaccaa gacgatcatg gatgtttctt tgcctgctgc tttgatcgct
    18121 tctaagtcag cttctgctac aagcgtatca ggtgtcactt gctgcccttc tgttactttc
    18181 acatcaaatg gcgtaccgcc taagtcaact gtgttgattc ccatatgaag caagatttct
    18241 aaaccatttg gcgttgtgat cccaatagcg tgtttcgttg ggaagacaga gctgactacc
    18301 ccagtaactg gagaatagat atgtccgttt tctggttcta caaagtagcc ttcacccatc
    18361 atcttttgtg caaaaactgg atcacttgct ttatctaaag aaacaactgt tccgtcagca
    18421 actgatgaga ggatagtcac actttcagct gaagtttgag gtactacttt ttcaggcact
    18481 tcatttactg tggctgtttc agtcgctaat tcttcttttt ctaaacggtc tttaggaata
    18541 ccgaagaaga atgtagcaac gaagccacca gcataagctg ccaataatcc aaggatgtag
    18601 cctaaccatt taccgtcaga gatcaatggg atcaaagcgg ctcctgaagg tccaatagcg
    18661 atagccccga catttccgat cattccgatc actgccccgc cgatacctcc accgatacaa
    18721 gcagtaataa atgggcgtcc tagaggtaat gttactccgt aaatcaatgg ttcgccgatt
    18781 cctaggatcc cgactggtaa agctcctttg atcatttctg ttaattcttt atcttttttg
    18841 cagcgaatcc ataatgccaa tgctgcaccc acttgtcctg caccagccat cgcaagaatt
    18901 ggaagcaata gcgtacgacc agtttcatca atcatttgca catggattgg agtcaaaatt
    18961 tgatgcagtc cgaacataac catcggcaag aataatgtgc ctagaacgaa gccggcaaat
    19021 gctccaccaa cgccaagtac ccagttgatc ccaccgacca tgccatcaga aatgaaacct
    19081 gctaaaggca tgatgaagaa aatttcaatc agaccgatgg caatcaatga aagagttggt
    19141 gtaacgatga tatctaccgc atcaggtact actttatgca gttttttctc aacgattgac
    19201 aatagccaaa cagcgaagat gacacctagg atcccacctt gtccagctgc aagagcttca
    19261 ccagtaaata agtttttcag tggtgcttcc ggattcatcc ctgtcagcat cacaacggcc
    19321 ccgataactc caccaagagt tggtgttgca ccaaaaactt gtgcagcatt gatacctgta
    19381 tagataacta ggtagctaaa catcccgttt ttcaagatgt tcatcacatc gatatattgt
    19441 tgccatgttg ctgcatcaag tgttccggct gtgactaagt ttgctaaaac tgcggcaatc
    19501 cctgccacga tcccagtacc gacaaatgca ggaatcattg gcacaaagat attagaaatg
    19561 tcttttaata ccgatttgat tttagatggt ttttgtttcg atttttgtgc cgctttcatt
    19621 tcttgtgccc gttcgtttac acggtctttt ccagatgctg atgaatttgc tgtagctgta
    19681 cttacagaag aaagtttttc tcctaatttc acgcctgcca aatcaaccat ttgctgtgct
    19741 actttgttca ctttcccagg tccgatgatg acttgaagct gttcatcttc tactacacca
    19801 agcactcctg gaatcgcttt taattcagag acatttacaa gatcatcatt gatgatcttc
    19861 atacgcacac gagtcatgca gtggacaatg ctttctacat tgcccatgcc gcctgcccct
    19921 ttataaattt gttttgccag ttcttccaaa gtcagttcgt tttttgccat ggtcttttcc
    19981 ccctcataag gtccctttta caaatccgtt tgcttcagct aattttgctt gagcttcctc
    20041 tttatcacta tttgtcaaaa tcatgacgat agccagctta acattttgat ctgcttcttc
    20101 aaagtacttt tcagctgttt cgtaatcagc ttctgtcgct tgcatgatga ttcgttttga
    20161 gcgttcgatt agtttttcat ttgttggttt gacatcgacc atcaagttat tgaatacttt
    20221 acctatacca atcatactga tagtagaaat catattcaag ataagttttt gtgctgttcc
    20281 tgacttcaat cgagtagagc ctgtcaaaaa ttctggacct gcatcgactt cgattgggaa
    20341 atccgcgtgt ttactgattt cggcttgttt attgcaagaa atcgttcctg tgacagcacc
    20401 gatcgatttt gcgtaatcta atccaccgat cacatatggt gtacgaccgc tagctgcgat
    20461 accaataact atatcatttt ttgtcaaatt cagcttgatt agatcttctt gccctagctc
    20521 ataagaatct tcggcccctt cgacagctac agtcattgct tcttctccac cagcaattag
    20581 acctatgacc atttccggtt ctacaccgaa tgtcggtacg cattcagcag catctaacac
    20641 acctagtctt ccacttgttc cggctcccat ataaatcaga cgtccacctt ttttaaagga
    20701 ctcaatcgtg ttagcaatca ctggttcgat acttgtcagt tctttttcaa ccgcagcggc
    20761 cactttctga tcttctttgt tcattagttg aacggcttga gaaatactca tctcatctaa
    20821 tccaaaagtt tcttggtttc tgcgttccgt tgttaggttc tctaaattga ttttgctcat
    20881 ttttatcttc cttcttttct tataaattga tagttcatac cagctttgat ttggttgatc
    20941 aatggtagct cttctttgat aactttagct actacattca ctttttcatc cgctggcaaa
    21001 tcatatttgg ttaattggat ctcacccata tagcgcaaat acttttcgtt atctaaagta
    21061 atagaccctt tcatacgcgt agctgtattt cttgctggga taggcggaat ctctcgaaaa
    21121 cgtgcatcta cgcttcgtat gacatctctt gcatcgtctt gccgattcgt atgcttccct
    21181 aatatatatt cgtaaaattc ttcatctaca ggttccgtat ggagcaagac tgtctgttct
    21241 ttttggaaag aagcaaattg ctcctgaacg ttttctgaca gacctgcatc tccgatgtaa
    21301 acaatatccg tgttacttgc taatagatct agagcgcctg caagaggatg gatccctcga
    21361 tgttcttcca aagttggcaa tccttgatat agtgggcctc tcaactctgt atctcctggt
    21421 acaaaacctt ggacaaggag accttgaatt ttgagccatt gatttttttt ctgataccat
    21481 tctttgtcca gccctgtttc gggacgtgga taataattat gccaagcttc aatctgtgag
    21541 aaatcagctt ctgcttcttt caattcatcg atatctttag gcgtgatcgt gctagcattc
    21601 aaactgattt tcaattgatg tgaccagtcg gcaatctgct gattggaaat atgataatcc
    21661 attcttaaac ctgttacacc aattgctttc aaaggcgtta gatcttctaa tgaaaaaccc
    21721 gctcgttcca atgcctctcc tgaaatatcg accatcaatt ctagtttatt ctttttcgct
    21781 tcgcttccta gttcgatcaa tcgcttttga tatgccgaaa catcatcttc tggtatatgc
    21841 atggaagtga aaataccgac aaagccattg gatgccattt ggcggatata ctgtttcttc
    21901 tcttctgaca aagaatcgtt cataaatatt gaaaagccaa acatattatc cctctttttc
    21961 tgtatccgtc ttcatttcta tgactagagt atatatctaa agaaacaaaa tttcaatact
    22021 tttattaaaa taattaaatt tcatttctaa aggttgagtt tatattatca aaagaattgt
    22081 cataacctaa aaaaggcact gctaagcaaa agcagtgcct tgaaatattt tatttaaata
    22141 acacaaaaac ataattactc ataagaaacc aattctgtat tatctaccat gaatgtaaag
    22201 aacccctcat taggcatcgg atgaaaataa gcattgatca ctcttatcaa ggcattatgg
    22261 caaactaaca gatagacatt ttcttcgtct tcgatgcatt ctttcaataa aggaacgatc
    22321 cgtgcataca catctaagac tgactcccca tttgggaatc gtacagcgaa cgctaaacgt
    22381 gctttttgaa agttctcatc tttgcttggc agtccatcat aatccccaaa atccatttct
    22441 accaatcgtt catcaacggt cagcggcaag ctgagacgat cagcaactgc ttgtgctgta
    22501 tctcttgctc gttgaagagg ggaatggatg attttcgtga ttggaacagg caaatctact
    22561 accttttctg ctagtttctg tgcctgcatc tgtccttttt cagtcagcgg cacatctgcc
    22621 cgtccgcaaa ctaatccttg tgcgttccaa gttgtttctc cgtgtctcgt tacataaagc
    22681 atgtgattct ctccttcgtt ggcaaagttc cccttaacca taccttgttg ttcttgagat
    22741 tacaagggtt agattctctc aagagaaata atctcagtag atactttatc caggaaggtt
    22801 tgatcatgtt ccactagtaa catggcaggt ttcacgttca agatcagttg ttctaattgt
    22861 tcttgattga agacatccaa ataattcaat ggttcatccc atgtatatag ttcagctggc
    22921 tgtgataaag atttagccaa ttccactttt ttccgttggc ccatactcat ctgctcgatc
    22981 ttgttatgaa aaacatctct ttccatccca agctttcgga ggttgttcaa aaatgcttgg
    23041 tagtctactt ggtttttctc tgcaaattcc gccaacgttc ctcgattgtc ttcataattt
    23101 tggcttgcat aactaatgct cagatgtttt ggctggtatt tttctcctat aaccttgccg
    23161 ttgaatgccc ccagaagata atggataatg gatgactttc ctgcaccgtt aggacctgaa
    23221 atcgccaccc gctgatgagg ctcgattgta aaatgaattg gctcgaataa cagattttca
    23281 taccctaatt gaagatcttc tacgcttaaa agtcgcttat ggtgagacgc ttggctattc
    23341 atcgtcaaag aatcgatata ttcgatatct tttaatagtt tttctttctc gctgatctgg
    23401 gtctccatcc gattcacgat tgctttggat cgtttcatcg tccgtgcagc atcagcaccc
    23461 actgctcctt tattcactcg tctagattca gtatcgatga atccgacttg tttctttgtt
    23521 ttatctcctt ctcgggaacg agaccattcg gctttttcag ctgctgtttt ctttagccta
    23581 ctgacttctt ttttcaattt ttcattttga gccatttcaa attcatcacg aagttttttc
    23641 tgttcttcat agatagagaa attcccttga taaagttcca gttgactttt ttcgattgct
    23701 aaaacatggt ccactacttc atcgataaat ccccggtcat ggctgaccac gatgaagcct
    23761 tgttttttct ttttcaaata agccgctact tgttttctac cagagatatc caaatgattc
    23821 gttggttcat cgattaacgg gaaatgagtg tcatccacaa ataaaagtgc cagtaggact
    23881 tttgtcttct ctccccccga tagtgtgctg aattctctcc ataagatttc aggatctgtt
    23941 tgcatcaatt ggagctctct ttcgatttcc catatctcaa aatccgtaat atcatttaac
    24001 acgtaatagg ttaaacgttc tttatctttt gtctgttgcg ggaaataggc aaattcttgc
    24061 tgatggatta cttgcccttg ataaggtagt ttgttttgta gaatattcag taaagttgtc
    24121 tttcctcgac cgtttcgtcc gataagtcct agtttccatt gtgtgtcaaa atttagattt
    24181 gcttgttcaa ataataatgt gccttggctg tcgtagccga atgtcagatt ttttatttcg
    24241 attttagaca ttttccatca cctcgtaaaa aaagaccagt cctattttca gaactggcta
    24301 aagaggatgc aagggttgga tacacacaaa aagacccata catcgaaaaa accaagccaa
    24361 tcccgaaaat ctgcacactg cttccttgtc tccacaaggt acagtctgaa tgcagctttc
    24421 atcaatcgac ttaatttttc aatgcacgga tccctcactt tgtcctatat ttgaaattac
    24481 gatagcataa agaaaatgat tttgtcaact acttcgggag gatggtgaaa aatctgctaa
    24541 gtgtctgtcc taagattttt gttttttcta atgtttgcct gtgttccatt gcttcttcta
    24601 aacctattgg acccaaatga atacttaaga caattgggag aaaatcagaa agtccttcta
    24661 gctcttggtc tctacttccg caaatagcga ttattggttt tcgatatttt tttgcaagca
    24721 ctgctacacc gtatggaact tttccgtgga tcgtttgctg atcgatccgc ccttctcctg
    24781 taatgatgac atctgcttgt tcgatctttt tttccaatcc agtgatttgg ctgactaaat
    24841 cgaaaccaga aaccatcttc gctcccaaca aaaaaagggc tccaccaata ccgcctgctg
    24901 ctccacttcc tggaatatca tctaaatcca cctcccagaa atcttttatt tgctgagcaa
    24961 tctttcctgc ttgtttatct aagaaagcta gcgtcgtatc tattcctcct ttttgtcttc
    25021 caaattgcca agcagctcct tgttgacctg tatatggatt ggtaacatct gcagcgataa
    25081 tcaattttac tttagaaaca agaacgtttt ttcgatcaaa atgaagtttt ttagtagata
    25141 ataaaggatt tccttctgtg tacccttcta aagaagcacc caaggattgg agtaatccta
    25201 gaccaccatc gctacacccg cttcctccta aagtgacaat aatttgttct acttcttgaa
    25261 gcagtgcatc tttaataagt tctcctaagg cataactgga agcaagtcgg atagtttgat
    25321 ccgttggttt atcaagtaga tctaacccca atgcttgtgc agattcgatg actgcggtgt
    25381 tttttccttg ccaagttgtc aataaatagc tgatttttcg ctgacggaat aagagatcca
    25441 tcccatcaat ggtacgccat gttcccctga gcgcgtaata gattgcttct gcagtatttt
    25501 ctccgccatc tgcaatcggc acattgattt ttcttccttt acgatcttcc cagccattta
    25561 gaactgcctg atttagttca aatgaagaag cacttccttt aaatgaatca attgccgcca
    25621 aaatatccat ctgtatccct cactttcttc ttaagtaaga cacttttttt catttttttc
    25681 aaacaaaaca atgctatttt cttccatttt tctatatact aaaagggaac tcattgaata
    25741 aaggagagaa aaaacatgaa aatcgcaata gctggggccg gagcaatggg cagccgaatc
    25801 ggattgatgc tacatcaaag cggaaatgag gtcctcttga tcgatcgttg gcctgctcac
    25861 attgaagcca ttcgtacaaa tgggttgatt gctgacttta acggtaaaga agtggtcgca
    25921 aaactgccaa tctattcacc agaagaaatc atcgaaagca acgaacacgt ggacttgatc
    25981 gtagcattaa caaaagccaa tcaattagat gatatgtttt gttcgatcca atccattatt
    26041 actgacaata cttatgtttt gtgtttacta aatggattag gtcatgaaga tgtactggaa
    26101 aaatatgtgc caaagaaaaa tatcttattt gggatcacga tgtggacagc tggtctagct
    26161 ggtcccggaa aagtcacatt gttaggtgat ggagaaatcg aactagagaa cttggaacca
    26221 gaaggcgaag catttacaaa aaaagttgta gaagtctttc aagaagcaaa tttaaatccg
    26281 atctatagcc ataacgttcg ttattccatt tggcgaaaag cttgtgtaaa tggaacattg
    26341 aatggtttat gcacgatttt ggattgtaat attgctgaac taggcgcgca aaaagcggca
    26401 gagagcatgg ttcgaacaat cgtatctgaa tttgcttcta tcgcagcaaa agaaggaatc
    26461 atactagatc aagaagaagt gtaccaacat attgcttcta cttatgatcc tgacaacatc
    26521 gggttgcatt acccatctat gtaccaagac ctgatcaaaa accatcgttt gactgaaatc
    26581 gactacatca atggtgccat ttggcgaaaa ggacaaaaat atgatattgc tacaccatat
    26641 tgtgcctttt tgacccaatt agtccatgca aaagaaggaa tccttggcgc agaataaaag
    26701 gctctctgat gagaatcagc tgaatcagca gctggttctc tttttttaag cccaataaaa
    26761 gatcaaaaag aaaacacaac tctcatgatg ccataagaat tgtgcttgtt tgtttcagta
    26821 cataatctct tcaaaatgta ggttttttat ttgttttcaa cagaggagag gtcatattcg
    26881 tattgctcat aaatagtgga atcgttagtg ataaatcgtt ttgcccaatt ttctgcatca
    26941 taaaattcca atagctctga taggattggc ttaaatggtg tatgcagaaa ttccggtaat
    27001 tcatctaaat cacgttctcg aagcaaaaaa atattttcag gacgataaaa atgataatga
    27061 gcaatcgctt gattatccgt cactagtttc ttttgaaaat acatggattc aaatgtccgc
    27121 aaagtcactc cttgctgacc ctcccgcaac agttccagaa tcccgcgcga ttcccctgtt
    27181 tttttcaggt actcttcata agtaagatat ccaggttgtt cattccctcg atgtggaaga
    27241 atgaaatagt tggtcgtaac atctcttttt ataaattcct gcttatagct ttctgctcgt
    27301 tcttttctcc cattattcgt tgcaccaaaa aatagatccg tctttggttc gctagaagaa
    27361 aaattcatat gtttcgaata aaatgaacta ttatgtttca gcccaagttc ttttgcttcc
    27421 aaaaaatcaa aatgataaat ctcatcgatt gccggttcat cttgtgcctg cttcagcatc
    27481 gcctcgtgtt ctgctttgaa gtgattccaa aaaaataaca caatttttgc attggtaaat
    27541 gggcgcacgc gtcgagccat ctccatatca aatccataat ctaaaaaaac gatcagctga
    27601 taatttgcca actgttgtct agctgattta atgaattccg actgtagtaa atcttttccg
    27661 gaccaaacat ctaaagagcg gatggaaggc aaaaaataaa gtgatttatt ttttgcaact
    27721 agcagtacgt catttttgtt catcttttcc gcctcacttt ctctgtcact ttttcctatg
    27781 atagcctgtt taaatagtaa aaagccagta aaacgtttcc tatatttgta tgaaaattta
    27841 acatttgtat gaaaatatga tgaaataaat cattcttcta ttaaaattcg cctaaaagtt
    27901 ttctgaatag ttgtaattgt ctgaaaattt gttatcatta gtcattgtta aatattgcta
    27961 tttattctta atataaattt tgattgtgaa tttaaaagaa agaggtcatt tatggaagat
    28021 aaaaaattga aaaaacaact tagctccaga catatcacca tgttagcttt aggtggagca
    28081 atcggtgcag gtctatttaa aggaagtggc gaagcgattg ggatcgccgg cccttctgta
    28141 ttgattgctt ttttgattgg tgggctgatt ttatatatcg tgatgaaagg tctgggaaaa
    28201 atcgttttaa gcggtggcga cacgcatcac ggattatctg gtttgatacg cccttatcta
    28261 ggtgctcatt ctgctgattt tactgattgg gtctactggt ctatgtggat tatcaatatc
    28321 atcgctgaag ctgttgcagc tgcttccttt ttgcagttgt ggttcccgca cattccagcc
    28381 tgggtatttg tttttatgtt ggctgtttta actacgatca tcaatcttta ttcggtacgg
    28441 ttattcgcag aaacagaata ttggctggct tttgctaaaa tcagtgtcat tatcttactg
    28501 atcatttttg ctacttatct tgtcggtcag caaatgttgg gcacaggcgt tttccctacc
    28561 cttcagcaat tgactgacca tggtgggttt actcctcatg gtatgaaggg aatcatcagt
    28621 tctttgcttg tagttatcta ttcttacggc ggttctgaac tgattgctat cacggtaagc
    28681 gaagcagatg atcctaaacg tgcgattcct aaagcaatca aaggtgtcat gggaagaatc
    28741 atttcctttt acatcattcc tttgtttttg cttttgattc tttttccttg gaatacttta
    28801 gcaggcacga atgtcagtcc ttttgtgatg gtatttgaaa aaatgaatat cccatttgca
    28861 gcggatatcg tcaattttgt gattgtcctt gctttgttct catctatcaa ttcgggtgtg
    28921 tatgcatctt caagaatttt gttcttccgt ttgaaggatc gaaaaggttc ctctagaaaa
    28981 ctagctgttt taaacaagca tcaagtaccg caacgtgctg ttttattttg taccagtgtt
    29041 ctttatcttg gtgtagctct ttcttatttt gtcggtgaca aattatttgg ctatctggca
    29101 ggttcccttt cgtatacagt attattgatc tggatcatta tcagtgcggc atcctttgta
    29161 ctggcactaa gaaaaggaac attatgggat aaaggaatca gcttttttgc attagctgtc
    29221 ttaggattga ttttccttgg tatcctgttc acgaactcca ttggggtaac tgtattgact
    29281 ggcctgcttt acctgttcat ttatttcagt tatcaaaaaa agaatgatgc gttcgtattg
    29341 acgaatgaat gaaatcgata gaaaatttta ttttatttat gtggaataga tggagctatt
    29401 gaagaaagga gttgtgacaa aagtcttgtt gcaactcctt ttt
//
LOCUS       DACKAC010000033        29109 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.033, whole genome shotgun sequence.
ACCESSION   DACKAC010000033 DACKAC010000000
VERSION     DACKAC010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29109
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.033"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..1030)
                     /locus_tag="I0B46_001487"
     CDS             complement(<1..1030)
                     /locus_tag="I0B46_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAR2083796.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1112..1801)
                     /locus_tag="I0B46_001488"
     CDS             complement(1112..1801)
                     /locus_tag="I0B46_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAR2083797.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="I0B46_001489"
     CDS             complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="I0B46_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAR2083798.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2328..2831)
                     /locus_tag="I0B46_001490"
     CDS             complement(2328..2831)
                     /locus_tag="I0B46_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAR2083799.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="I0B46_001491"
     CDS             complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="I0B46_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAR2083800.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="I0B46_001492"
     CDS             complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="I0B46_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAR2083801.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4799..5467
                     /gene="sdaAB"
                     /locus_tag="I0B46_001493"
     CDS             4799..5467
                     /gene="sdaAB"
                     /locus_tag="I0B46_001493"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAR2083802.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5480..6352
                     /gene="sdaAA"
                     /locus_tag="I0B46_001494"
     CDS             5480..6352
                     /gene="sdaAA"
                     /locus_tag="I0B46_001494"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAR2083803.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6443..7462)
                     /locus_tag="I0B46_001495"
     CDS             complement(6443..7462)
                     /locus_tag="I0B46_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAR2083804.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7635..8522)
                     /locus_tag="I0B46_001496"
     CDS             complement(7635..8522)
                     /locus_tag="I0B46_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAR2083805.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="I0B46_001497"
     CDS             complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="I0B46_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAR2083806.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9245..9415)
                     /gene="secE"
                     /locus_tag="I0B46_001498"
     CDS             complement(9245..9415)
                     /gene="secE"
                     /locus_tag="I0B46_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAR2083807.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="I0B46_001499"
     CDS             complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="I0B46_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAR2083808.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9753..10397
                     /locus_tag="I0B46_001500"
     CDS             9753..10397
                     /locus_tag="I0B46_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAR2083809.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10453..10956)
                     /locus_tag="I0B46_001501"
     CDS             complement(10453..10956)
                     /locus_tag="I0B46_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAR2083810.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10969..11015)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11553..12518
                     /locus_tag="I0B46_001502"
     CDS             11553..12518
                     /locus_tag="I0B46_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAR2083811.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12617..13789)
                     /locus_tag="I0B46_001503"
     CDS             complement(12617..13789)
                     /locus_tag="I0B46_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAR2083812.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13776..14921)
                     /locus_tag="I0B46_001504"
     CDS             complement(13776..14921)
                     /locus_tag="I0B46_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAR2083813.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14935..15867)
                     /locus_tag="I0B46_001505"
     CDS             complement(14935..15867)
                     /locus_tag="I0B46_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAR2083814.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15984..17063
                     /locus_tag="I0B46_001506"
     CDS             15984..17063
                     /locus_tag="I0B46_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAR2083815.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17060..17689
                     /locus_tag="I0B46_001507"
     CDS             17060..17689
                     /locus_tag="I0B46_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAR2083816.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(17799..18026)
                     /locus_tag="I0B46_001508"
     CDS             complement(17799..18026)
                     /locus_tag="I0B46_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAR2083817.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18129..20717)
                     /locus_tag="I0B46_001509"
     CDS             complement(18129..20717)
                     /locus_tag="I0B46_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAR2083818.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20737..21396)
                     /locus_tag="I0B46_001510"
     CDS             complement(20737..21396)
                     /locus_tag="I0B46_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAR2083819.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21445..21948)
                     /gene="trmL"
                     /locus_tag="I0B46_001511"
     CDS             complement(21445..21948)
                     /gene="trmL"
                     /locus_tag="I0B46_001511"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAR2083820.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22169..23035)
                     /locus_tag="I0B46_001512"
     CDS             complement(22169..23035)
                     /locus_tag="I0B46_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAR2083821.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23144..23785
                     /locus_tag="I0B46_001513"
     CDS             23144..23785
                     /locus_tag="I0B46_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAR2083822.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23847..25208)
                     /gene="mgtE"
                     /locus_tag="I0B46_001514"
     CDS             complement(23847..25208)
                     /gene="mgtE"
                     /locus_tag="I0B46_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAR2083823.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25226..26125)
                     /locus_tag="I0B46_001515"
     CDS             complement(25226..26125)
                     /locus_tag="I0B46_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAR2083824.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26127..26924)
                     /locus_tag="I0B46_001516"
     CDS             complement(26127..26924)
                     /locus_tag="I0B46_001516"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAR2083825.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26902..27606)
                     /locus_tag="I0B46_001517"
     CDS             complement(26902..27606)
                     /locus_tag="I0B46_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAR2083826.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27737..28312
                     /locus_tag="I0B46_001518"
     CDS             27737..28312
                     /locus_tag="I0B46_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAR2083827.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28430..29107
                     /locus_tag="I0B46_001519"
     CDS             28430..29107
                     /locus_tag="I0B46_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAR2083828.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8565 a         6169 c         5232 g         9143 t
ORIGIN      
        1 atacagtatt atttcggttg cttgctgctt cacctagccc ttgtactcgt gtttgattgg
       61 cgacttgacc aaattgattc ccgacgtcta ataaaataat agctaaaagc aaaatggcca
      121 aatggcttcc gccaaccaat aaagcaataa agcttattgt ttgcataaca agtcccatat
      181 acacgatttt tctttcagaa taagaatctg ccagtcgtcc cacgataggt gcagcaactg
      241 ctccagacaa accaaaaatc gctaatatcc ctgcttcgaa tgttccccaa ttgtaggcgg
      301 gactgctgat ataaaaaatc aacgtagacc aaaagatcga aaatgttcca aacatgaaaa
      361 atccactgac tgccgcttct cttaatagac gttgagaagc aactaaatgc ggcaaacttt
      421 taagtgaatc aaggtatgaa acagctgtac tactttgctg gacagaacgc ggcattttcc
      481 agtaaagcag aagtgtaaga attccgactg cacataaagc aaataaatag atcatccgcc
      541 aagaaaaaag actggcaaca ataccagata ccgtccgaga aagcaaaatc cccacgagta
      601 aaccgctaag caatgtaccc atgaccttcc ctcttgcttt tggtcccgct agtaccgcac
      661 cgtaaggaat gatgatttgc ggcacgatcg aaagtaatcc tattaaaaaa gaagcgcatg
      721 caaataacgt aaacgaaggc gcaaaaaaag cggccaacag agagacggca gataacgcta
      781 ccattcgaat gatcaatttt ggacgatcca ccacgtcccc taaaggaact aaaaacagta
      841 accctaacgc atagcctaat tgggtcaaca tcgtcaatat cccgactgca cttgtgctaa
      901 cagataaact agctgctact tttgttccaa tcggctggat gtaatacata ttggcaacaa
      961 caacgccaca agtgattgat aatagtaaaa taagcgaagt tgttaaaggt gttttttcat
     1021 tactcttcat tgatttcccc catttcctat cttagtttac aacttatgga aaagattgga
     1081 aatagaaaaa tacaactatt cgccacactt attatttgtg cttttgtttt ccttgttttt
     1141 tcttgcgatc tttataaatg aagacaacta accctactag cacagtaaca cttgcaacca
     1201 acgtgactgc agttttgtta tatccgtgaa acagagcttc tccaccacac gcatacaaca
     1261 cagacgaagg gatgactcca atcacgatcg acaatatgat ttgctgtaga ggcaatttca
     1321 tcgcatctgc agcaaaattg accatagaag atggaataac cggtatcata tagcctaaca
     1381 ttacacctat ttttggatgt ttcatttgtc ggattgcttg cacccaatga ttcgtttcgg
     1441 ttttcttatc taaaaatttt aagcgatgca taaggaaaat tgcagtgatg ttaccaagag
     1501 cattacccaa aatgttgatg atcgcacctg tcagtggtcc aaagctcaaa ccagcaatga
     1561 ctccaactac tgaagtagga atacctggaa cagcacacat caccgctgtc aatacgatca
     1621 agagtatcga tgcaaaagtc ccatgcgaac gaatatgtgc gagtagctct gccttactcg
     1681 ctctcggatt taaaaataat tggatgtctg tctgatattc taaatacaac cgatagccca
     1741 ctaccagtat cagaatgatt cccaacgaga tcaacatcaa tcggattatt ttctttttca
     1801 tatttcctcc tgaaaaaaat ctatattcat taaataatag tttaacataa aaacaaaaaa
     1861 gcaccgaaat ccaacatagg atctcagtgc ttttaaatag taaaaattat ttaacagtta
     1921 cagatgcgcc aacttcttca agtttagctt tcaattcttc agcttcttct ttagatacgc
     1981 cttctttaac tggtgcagga gcgccatcaa ctacagcttt agcttctttc aagcctaaac
     2041 cagttgcttc acgaactgct ttgataactt taactttttg gtctccagct gcagttaatt
     2101 ctacagtgaa ttctgtttgt tcttcagcag cagcagcgcc accagcagca gctacagcta
     2161 caggagcagc agcagatacg ccaaattctt cttcaatagc tttaactagg tcgttaagtt
     2221 ctaggatagt tgcttctttt aactcagcaa cgatgttttc aatgttcaat gccattataa
     2281 tttcctccgt ttaattgttc ttttttattt aatgctttat gcggcaatta agctgcatct
     2341 ccatcttctt tgtctgccac tgctttgaca gcgtaagcca cgttgcggac tggcgcttgt
     2401 agtacagata gcaacataga tagaagtcct tcgcgatttg gcaattttgc caatgctgtg
     2461 atttcttcca atgaagagac tttaccttcg atgataccgc ctttgatttc taatgctttt
     2521 gcttctttag caaattcgtc catgattttc gctggtgcga ctacatcttc gttactgaat
     2581 gcaacagctg taggtccagt gaatacttcg tccatgcctt caaggccagc ttttttagct
     2641 gcacgtgaaa gaatagaatt tttgataact ttcatttcta cgttagcatc acgaagttgt
     2701 ttacgtaaac gtgttacttc atcaactgtt aatccgcggt agtcaacgac tactactgat
     2761 gcagctgctg taaatttttc gcttacttca tcaacgattg ctgctttttt tgcgataatt
     2821 gcttcactca tttttgagtt tcacctcctg aattttgatg gaagagtttt ttgcactgaa
     2881 aaggcaagtc acttttatag ttactttgct caaccagtat tattcatcac ctattcactt
     2941 cgttcatagt gattctactc gctttgctcg aaaggaaact cctttcgctt tgctcaacca
     3001 gtattgttca tcatctcttc acttcgttca tagtgattca caataaaaaa ctctatgcca
     3061 ccgcagacat agagggacta ttgaatatat tcaataaacg tcctcggtag gaaattaagg
     3121 cttgatgcca cctactgtct tcggtacaag ttaattatta acctcaacta gcttagcatg
     3181 ctaaactagt tgagtcaaga attatttttc agttggatca atcaacattc taatatttaa
     3241 ttagaaagaa gcttggtcaa catggatacc aggtccaaat gttgttgtga tcgttacatt
     3301 tttcatgtat tgacctttag ctgctgatgg tttagctttt agcaatacat catggattgc
     3361 tgcaaagttt tcaactaatt tttcatcgtc aaatgaaact tttccgattg gtacatggat
     3421 atttccagct ttgtccacac ggtaagttac tttaccagct ttcacttcgt ttactgcttt
     3481 tgttacgtcc attgtaactg taccagtttt agggtttggc attaagcctt ttggtcctaa
     3541 tactcgtcct agacgaccta cagtagccat catatcaggt gttgcaacaa ctacatcaaa
     3601 gtcaaaccat ccgttttgga ttttttgaac catgtcgtcg tcacctacga aatctgcacc
     3661 agctgcttca gcttcttttg ctttgtctcc tttagcaaaa actaaaacag tttgtgtttt
     3721 accagttccg tttggtaata caacagcacc acggatttgt tggtctgctt ttttagggtc
     3781 tacgttcaaa cggtatgcaa cttcaactgt tgcatcaaat tttgcaatgt ttgtttcttt
     3841 tgctaaagct actgcttcgg caacaggata aactttactt gaatcaactt tttttaatgc
     3901 atcttgcatt tttttgctct ttttagccat tttggttcct ccttgattgt ggttataacg
     3961 gttgtacctc ccacgtgttc tatatctttc aatatagagc caactgagaa gctgctgtct
     4021 tagggtgtat agattattct acagtgatcc ccatgcttcg tgcagttcct tcaaccatgc
     4081 gcattgctgc ttcaacgtta gctgcgttta ggtcttccat ttttaattca gcgatttctc
     4141 ttacttgatc gctagaaact ttcgcaactt tatttttgtt tggttcgcct gaaccttttt
     4201 cgattttagc tgcttttttc aatagaacag cagctggcgg tgtttttgta acgaatgtga
     4261 atgaacggtc ttcgtataca gaaataacaa ccggaatgat caaacctgct tgatcagctg
     4321 tacgagcgtt gaattctttt gtgaatccca tgatattgat acccgcttga cctagtgcag
     4381 gacctactgg tggagctgga gttgctttac ctgcaggaat ttgcaattta acgatttttt
     4441 ctactttctt tgccacgaga catacctcct taagtccgtg atgtggttaa ttggagatgc
     4501 atatttctcc tcccacgttt tatctgatct gaaaaaatcc agacaaataa tgtgcaaaac
     4561 aatgcacact ggtacagtat agcaaattta tttgtttttt caagattttt ttctgctatt
     4621 tttacatttt gcttgaataa acgctaaatt tattctaaaa aaacaacaat ttttcgttct
     4681 catttcttct tttatgttac acttgcgcta gcatggttca acagatgaaa gcaaacaata
     4741 acacggtttt ttagaccctt gtacaaagat tcctacataa caaaatgagg tggagaaaat
     4801 gaaagattta aggtatagaa gtgtctttga tattattgga cctgtgatga ttggtccaag
     4861 tagctctcac actgccggag ctgctagaat cgggaaaatc atacgtagta tatttggcga
     4921 acagcctgat tctgtagata tttatctata tgaatcattt gcaaaaacct atcgggggca
     4981 cggaacagat attgcattag ttggcggcct gttagggatg gaacccgatg atgaacgttt
     5041 agctgattct ttgaaattgg catatgaagc cggtatggaa gtcttgtttg ttcctaagag
     5101 tgaaaaagcc gaacatccaa actctgtaaa gatgcttcta aaaaaaggaa cacgaaagct
     5161 ttcagtcact ggcatttcga tcggcggtgg caatatccag atttctgaat tgaatggatt
     5221 caaaatttct ttaagcatgg gtactcctac cttgatcgtt gtccaccaag atgttccagg
     5281 tatgattgct aaagtgacta gtatactttc tgagaccaat acgaatatcg gcactatgac
     5341 agtgacacgg gaatcaaaag gtgaaaatgc cattatgata attgaagtag atgatcctca
     5401 agtagaagaa acggtaaaaa gactcaaaca gctgccaaat atcgacagcg taaattattt
     5461 cgattaagga gctaaaaaaa tgtttttatc gattgaagaa ttagtagatc aagcaaaagc
     5521 ttatccaagt gtagcagaat tgatgattgc agttgaaatg gaaatgagtg gccgttcccg
     5581 tagtcacatc atcgagatca tggaaaaaaa cctctcagta atggaacaat caatcaacga
     5641 gggaaaagaa ggtgttacct ccgtcacagg aattaccggt ggtgatgcaa aaaaattaga
     5701 tgattattta aatgaggcag actttttaag tggagaaaca atcttaaaag ctgtacgcaa
     5761 tgctgtagct gttaatgaag tcaatgcaaa aatggggttg atttgtgcta caccgactgc
     5821 cggaagcgcg ggtgttgttc ctggtgtgct gatggctgtc cgagatcggc ttgatttgac
     5881 acatgaacag caattggaat tcttatttac cgctggcgct ttcgggttag tcatcgcaaa
     5941 caacgcttct atcagtggtg cagaaggggg atgtcaagca gaagttggtt ctgcaagtgc
     6001 aatggcagca gcagctttgg tttgtgcaaa aaatgggact cctcatcaag cagcacaagc
     6061 agtagccatt acattgaaaa atatgatggg actgatttgc gatcccgttg caggcttagt
     6121 tgaagtcccg tgtgtcaaac gtaatgcttt aggctcttct caagctttta tctctgctga
     6181 tatggcatta gcgggtatcg aaagtgtcat tccgccggat gaagtcgttg ctgcaatgta
     6241 ccaagtcgga cgtcaaatgc cgtcgatttt caaagaaaca gcagaaggtg gcttagctgt
     6301 tacgccaact gctaaacgac ttgcggaaga gattttaaat aagagggcgt gataaagctc
     6361 cttaatttcg agtattaaga gattagttat cttcataaca aaaaacgaag agaggactaa
     6421 ttgcccttct cttcgttttt tattaatcca tcaatttcgt attttttact tcatcgatcg
     6481 tttttgttcc tgcaagttgc atcgtgatcg acaattcttt gttcaggtgt tcaaaaacag
     6541 atgttactcc ttctgcccca cctagattca acccataaat cactggtcgg ccaatcgcaa
     6601 ctaagtcagc accacttgcc agagctttga agacatgctc tcctcgacgg acaccagagt
     6661 cgaaaatcac tggtacacgt ttgttgacta cttcggcaat ttttggcaat acttcaaagg
     6721 acgcaggacc tccatctaat tgacgtccac catgattgga tacccagata ccatctgccc
     6781 ctgcagagat agcgattact gcatcttctg gcgattgtat tccttttacg ataactggaa
     6841 gatgggtgat ttctttgatt gttttgatgt catctggtgt caagccttgt tttgcagctg
     6901 cataaatttc tgcgattcct tttccttcgc cattaccact tgcactttgt tcgctatacg
     6961 cagctaagtt aggcattggt aatggaaatt ggaattgatt gatgacgtct tcttcacggt
     7021 aaccaccgag cgttgaatct gccgttaaaa tgatagcttt agcccctgct tctactgctt
     7081 tgttcaaaat gaactcattg aatctgtcgt ctttactcat atataattgg aaaaactgcg
     7141 gtgcgcctgg cgctgcatcg gctgcgtctt tgattgtcgt atttgcataa gtgctgatgg
     7201 aaaagattga tccagcagca gctactcctt tagctgtatc agcttcccct ttttcatgtg
     7261 ccaatccttg ggccgctgat ggcgcttgga taatcggtgt atctaattca attccgaaaa
     7321 cagatgtatg caaatcggct gagtcgattc cgcgtaatat ccgtggcatg attttctttg
     7381 tattaaaaga agtcgtattt tctttcatcg tccactcatc ttcagaacca ccgcgaatat
     7441 agccaaaagc tcctgcttcc atgcgctctt ttacacgacc ttctagttag gcaatgttta
     7501 caatatcgat tggatgttct tcattacttg tatgataagt catagtagat ccttctttca
     7561 tcttcttgat tacatataaa tcatactaaa cttacaaaaa gtaagcaaac atttactctc
     7621 agatactttt atcgctattc tttccaaata aatcgactta caagctgatc tacttcttgg
     7681 ttttcatgaa gcatactatg agaggcattt tcacctgtca ctactttttc ctctatttca
     7741 tttccgcgtt gcttcaacaa cgcatagacc gctaatgcac tgtttaacgg tactgttcca
     7801 tcactcaagt ctgtttcatc tagttgacca gcaatcaaga aaaatcgagt ggttatagga
     7861 atattaccga tcatttgctg gtaatcttga tagcggttag aaacgacagc aggccccttt
     7921 gctaactcat ctgtcagaga ctgcgcacta tcatcgacaa aatcattaaa aggcgcgcca
     7981 attgctgtta atgtctgaac ttgtggcaat gacgtatcct gtccatacgt tcctaaatag
     8041 cgtagtgcac taactcctcc catggaatgc cccactatgt tgactttatc tatgccgtag
     8101 ttttctttca gatacaaaag acaagttttg atccattctg cttgattcca ctcgttattt
     8161 ttattatctt caaacaatac ttggaccatc ggattgtctg cttttccaga caaatctcct
     8221 gttgcttgta tttgtccatc tgttcctact gttaaaacca attcttttac tgcttgctga
     8281 tttttttcca aacgccgcaa catcccttta aaggaatttt ctgtcccgct gtagccgtgg
     8341 aaaaaaagtg ttggaatagt ggttttttct atcactttac tggattctgc cgttttttct
     8401 ttatcagaaa cttcagtagt atttatcttc gtcgtctggc aaccacttaa cgcaaacaaa
     8461 ctgataagga ctaaagctgc tgcagttgca cgtttgcata aatttttgat tggttctgtc
     8521 atacacctct tcctttccat atagaaaaaa gaaagacttg aaaggccaat catatgatcg
     8581 acatttcaag tctctttatt aatcgatttt atctacttga tcgaaatcaa gttctgtact
     8641 ggtttcacgt ccaaacatat cgatgttgac tttcaatttt tctttttctc cgtcaacttc
     8701 tgttacttgt ccttctaatc cagcaaatgc gccttcgatg attttcacga cttcgccaac
     8761 ttctacttct agatcatttt gtcgcaggct cataccgata gaacgcaaga tatgattgac
     8821 ttcttctgga agtaatggtg ctggtttgct tccggctccg tgtgaaccaa cgaatcctgt
     8881 cacaccaggt gtgttgcgga caacatacca tgaagcgtct gtcatgatca tttcaacaag
     8941 tacgtatcct gggaatgttt tatgaacgat ttctttttct ttcccatttt tgacttcagt
     9001 ttctttttct tcaggaacaa cgacacggaa aataaagtcg gccattttca tactttgcgc
     9061 acgagattcg atatttgctt ttactttgtt ttcatatcct gaatatgtat gcaagacata
     9121 ccagtttttt tcaaaagatt ccattgaagg ctcctcattt cattgtaaaa taaaaaaacc
     9181 tcagtacacc cgaagttttt ctttcgcttc attatagcac taaaagtacg ttgtggctag
     9241 cgagttattt taaaatccag ccaaataagc tttggatggc tgtatccatt acgtaaaaca
     9301 tcaatccgaa aataatagaa gtttcgatga ctactaacgt atctttacgg agttgttttc
     9361 tgcttggcca agttacttgt ttgatttcat cttttacgct ttttagaaat ttcatgagac
     9421 aacctccctt gctactgttt atttcgtttc tttatgtaat gtatattgat tacaatattt
     9481 acaaaactta ttgatttcca aacgttcgcc gctttttcct tcactgactg attttgtgta
     9541 gttgcgagac ccacaaacgg aacaagcgag tgctgctttt ttctttgcca tttacttctg
     9601 cccccttgct gagaatcaga cttatacttc ataacagtat cacgtaactt tttacttgtc
     9661 aatttgtgaa ataaactatc tattgttttg gataaaatta tgatatgatt aatttgaaat
     9721 taagctaaaa tgagacaatt ggaggtatag cgatgctatt aaaaacactt gtatataaga
     9781 aaaaagatct gacaaccgtt acagagacta gtacattaga agaagcatta aaaattttag
     9841 aaacttccgg ataccgttgt gtgccgatct tagattcttc aggtaatatt ttccgtggaa
     9901 acatttacaa aatgcatatc tatcgtcata aagcaaatgg cggtgatatg acgcttcctg
     9961 tcactcatct tttgaaaaac gctacaaagt tcatttacct ggatacttct ttcttcaaag
    10021 tattctttac gatcaaagag cttccttata ttgctgtcct tgacagtgaa aatcattttt
    10081 atggtatttt gacacatagt acgttgctta atatgctctc tcaatcttgg agcgtagaac
    10141 aaggaagcta tgtactgaca attgcttcca caggaaagca aggagatttg gctgctattg
    10201 caaaaatcat ttcaaaacac agcagcattg ccagctgtat tactcttgat atcgaaaaag
    10261 acgaatacat tcgacgtaca ttgatcacgc taccggctaa cactgaagcg aaagtatgtg
    10321 aaacgatcgt caaccactta gagaaaaaga attataaagt ggtagaaatt gaaaatttgc
    10381 aaaaccaaga agcataatca aaaagcgaaa aaagaaaaca gctaatctgc tttctttttt
    10441 cgctttttat aattagacac gcttttcaaa atcaagcgcg cgatctaaat aatacatcaa
    10501 tggtgctgaa acagccatga cgataaactc acttaatgca agcgtgccat aggttgtcca
    10561 aaatgcttgg ccacctgttg gaactaacat ataagcaatc agtcccatgc tgatggtaaa
    10621 gaagatcgtg ttcaatacta aacgcatctt tacatttttc actttgtttt gcaataaagc
    10681 tgtcaggcct aaagaaagca atgtttggcc gccaccaaaa accacatcca tgataccaaa
    10741 accaaagaag gcattataga cgatgacccc gcctaaaact ccccaaagca atttacggtt
    10801 aaagacaact aaatgattca aactttctga aatacgaaac tggatcggcc ctgaggatac
    10861 aggcgcaact aaaatagtta acgctagata aagagccatg atgatgccat ttacaacgat
    10921 tacggataac cgttgtctgc ttttcgtttt ttgcattttc tctcctcttt tctcctagtt
    10981 tttttaacgc gggatggttg atgaaccgcg gttatgtgaa acaaacaata attataacag
    11041 acttttcatt tatttcaatc cgtcttatct gcttttaaaa aagcaaaagc ttaaacaatg
    11101 taaacgtttg ctaaaaatcg tgggatatgg tataaagtaa gtgtaagggg tcataagaat
    11161 tcccagtaaa gcagacgaag taggatctgc aagaacagaa ttgttgtgaa acttaaataa
    11221 ataaaaaagg atgtgggaat ttgagcaacc tcttactaga gaacggtcta gcaagcagct
    11281 gaagatctaa gtggacatgc cgaaccttgg aacttctcct tacgtaagta atggatcatg
    11341 cgcttgctag gcttttttgt gtttttcggt cttcggacct ttttcatttt cctagacttc
    11401 tgctataata accaagaatt aattttgtaa cagatttttt tgaaaaaaca agtttctttc
    11461 attcaaaaaa tagaaaaagc ttgctaatta ttggttaacc agcaaacagg agtattaaaa
    11521 tatcttgttt tattgaatag caggaggaaa tcatgaaatt ccattatcat atattgatca
    11581 atccatcggc aggcagcgga aatggctata aagtagcaga aagaatttta ccggtattaa
    11641 aaaataagca tattgactat acacttcatt atagtgaata caaaggacac gatgctgaga
    11701 ttgctgaaac tttggcaaaa gaaacattga ttccttggat agaagaagaa cagaaaacaa
    11761 tcgatacttt cccccttttg atcatcgtag gaggtgacgg caccttacat caggtacttg
    11821 atactttcta tcagatggaa gtagaattcc cagttgctta tattccagca ggttctggaa
    11881 atgattttgc tcgaggggca ggccttccaa aaaatcccaa aaaaggactt cagctgattt
    11941 tagcagctca atcaccagaa aaagtccaca taatggctta tgaagaaaaa ataagtgaaa
    12001 aaaaaggaat agctgttaat aatttcggta taggattaga tgcagctatc gtccatgcga
    12061 caaatcattc ttccacaaaa aagcgattga acaaatacaa cttaggatca ttttcgtatt
    12121 tattctctat tctccgtgct ttattcactc aaaaaggatt tccaattctg gtagatgcag
    12181 gaggaaaaca attaagtttt tcaaacgcat ttttatgtac agcaacgaag catccttttt
    12241 ttggcggagg aatcgcgatt gttccaactg ctgatgcgtc taaaccagtc attgattttg
    12301 tcgtggtcga acgaatcaat ttctttaaaa tcttgtggct cattctttta cttatacaga
    12361 aaaaacaatt gaaatcgaaa tatttccatc actacacaac aaacagatta cgtatcgttt
    12421 caacaacacc gcaacatggt caagaagatg gagaagaaat ggaaaaacga ccttttgata
    12481 tcacaataac cacaactaaa cagttgtttt ggtgttaata aaagaggttt tgtcagaagc
    12541 tacatctgga acaccgttta ttcggaaaaa aggactgtga cacgactttt gtcacagtcc
    12601 tttttttact atgcagttaa ttctttgccc aattgggttg ccatcgatag tttctttctt
    12661 tacccaaaat aaattggatc attgaaaaca ccgcagcaaa agcaactaag acacaaccat
    12721 tgaaccaaaa agattctgca aatgtttgat tgtaactgag tgtttcactg atcaagttat
    12781 tattttgaac aaaccaatat gttggtgtga atgccgctat tttgctaacc atttctggca
    12841 agtattctgc tggtacaaac accccaccga taaagcaact gcctgtgata aaaatatctt
    12901 tgatcccacc gctggtttct gagtttttta ccaatgaagt gatacaagcc gaaaagctaa
    12961 tgatcggcaa taaaaacaag aaagtattta aaatatgata ccataccgtt atatcccacg
    13021 aggtgtggat cactaacaga accattagaa gtaagaagcc actgaacaac agcagcgagt
    13081 aactaatcaa actaaaggtt actttgcgat tcaagtttcg tttggtaatg ggcgcactct
    13141 ggtttcgttt actgatctct ttgcgattaa acgctagatt gatgacgctg aaaccgctaa
    13201 aaatggatag aaacaaccca taagacaaga aattaaaggt tgtgcccgtc aacagctttt
    13261 tcatccgctg actatatccc gcacttagct caacttctcc agtaaccgag agtgccattt
    13321 gtgtctgttc catagctgca tcgattgttt tatcttcttc ttgaaacaga atcagtgttt
    13381 gcaaaaaggt gttcacttgc tgtgtaacaa ctgttttaga aaaggcatct gggcgactct
    13441 gtatcttgat cgtcgccatc tttcctactt cgacttgttc ggtaaagtct gcaggcagat
    13501 gtaagataat gtttacacga ttaaaataaa gagcatcatc gatttctttt tgtgaggttc
    13561 catccaatgt aacgactgtt tccttgtcat tcaaatattt acgaaggcca tcgactacag
    13621 gcgactcttc ttcactaatc aaggcgattt tcgcatcttg cagagaggta ttttcaacat
    13681 caatattttg cgcaaaaaag aatgtggcaa caacagtgat aattacacct atcagtaaat
    13741 tcgatttatt tgctttaagc aatgtccaaa tcgttttata aagcgtcata acgcacccct
    13801 ctttccatcc aagcatttcc taacgcaaaa aggaaaataa atccgatcaa ccatagaaga
    13861 ttggtataaa aactagccaa atcagtgtag aaataaagtt tatacaaact ttcgctgatc
    13921 aagttgacga gatttaattg accaacaatc ggaatatgaa gatcaatcca atacttcact
    13981 tgttccgacc ccatcatacc agcaaaaaag ctcatcgcaa tcgtcactga aatcaagatg
    14041 ctatcctttt gcaccagatt catttttggt atcaaattac caaccaatgt acctaacaaa
    14101 atcgcacaca acgaccccag agcggtaaca atcatcaacc attgccaacg ttcaccaaag
    14161 tctacttgat agataaaacg aaagacagct aacataatat aaatctctgt caaaacaatc
    14221 gtaaagcttg ctgccaaatt agcgattgaa actaacgatt gctttctagg aatcaaggac
    14281 aagcgaatac catttgacga ttgatttgct tgctgatcct gtacattgcg gaccccccac
    14341 atcgtcccgt agagaatcgc cattccaatc agagtgaaga aataaaaact tttgaaactc
    14401 atattacgac ctgcatgaac ttcttgaaaa gatacttctt gttcaaaaac agtcatgatg
    14461 ggcgcgagat caacgttctc cacttctgcc actttactcg aaattatacc aatacgctgt
    14521 aaaaattgat ccatgacgtt tttcatcaac gtctgactca ttcctgcttg acccacatgg
    14581 agcgaaacag atgaaccatc aatgagataa aaaccagcca aatcatctgc atttagcttt
    14641 tcattcgctt ctacttccga tagttcctgt acttcaaata atggtttatc attgacttcg
    14701 acttgttcta agactgtggt aaaattttcc gaaagcgctg tttgatccgc tttgattaca
    14761 attccagtat taatcgtttc aagattgcca ccttgatcaa tctcgccaaa ggccgccata
    14821 aacatcaatc ctaaaagaat tggaaaggcc aatgtccaaa acaacatgga tttatttcgc
    14881 agcagtattt ttgtgcgata tttataaaaa tgccaaaaca ttatttcccc tcctctagtc
    14941 tctcaaatcc ttgccagtaa tctctaaaaa gacatcattc aaggacgctt ctttggttgc
    15001 tatttttccg taactgatct tttcttcttg taagattgta agaatcggca acaatactgt
    15061 atttagttca gtagttgtta cttggagctg acctttttta taagcgattc catcagaagc
    15121 tattgttcgc tgaatcgctg acaacacatt ttctgggagt aatgggatgt cgatcgtcaa
    15181 acgatccgcc gtccgtacca tatcttttag agattctttg gttcctttag caatcacctg
    15241 tccttgatcc atgatcacga tctggtcgca aagaatttct acttcttcca tgtaatgtgt
    15301 tgtatagaca atggttgctc cttgtcgatt cagctccttg atactttcta aaattttatt
    15361 ccgactttgg gggtctacag ctactgtcgg ctcatctaaa aaaattaacc gcggcttgtg
    15421 ggcaatccca catgcgatgt tcaaccgacg tttcaagcca ccactaagtt ctcctggacg
    15481 aaacgaaaca tatttttcta atcccactaa ttgaatcacc tcttcaatca aggctttcct
    15541 tttctctttt tcggaaacat ataaaccgca aaaataatcg atattttctt gcacacttag
    15601 ttcgtcaaag accgcaacgt cttgaggaac gaccccaatc attgccttga cagcataatt
    15661 ttcaggagtc attgttgtcc cgaacaattt aatggttcct ttgtcataat ttaacaaaga
    15721 aagcatacag ttgatggcag tcgatttccc actgccgttt ggacctaaca gacctaagac
    15781 ttctccttct tgtacttgca gatcaaaatg attcaatgct acaaaatctc catagcgttt
    15841 gactaattta tcaatttcga taatcatcat gaaaaactcc ttccaaattt cttgttgatc
    15901 tgactataat aaagtgcaag aaaagaaact agtgacgatt ggctaaacca cacatgacat
    15961 ttgtcacaat gaaggagtct gctatgcata aatctcggct actcaacgaa actagctttc
    16021 tactggttat ctcattgctt ttgctggtaa gtcaaggatt cgttgctatg caagttatct
    16081 atttattgat cgccgtttta ttcactggtg tcagttattt actgcctaca aagccaaatt
    16141 ggattttcca agttattcta tttcttttat gtgccttttt acctgatctt gtcctgtttc
    16201 tgccagcgac gatgcgagtg atcgcccctg aagaaagcca ccttgattac ttgtttttgt
    16261 taggtttttt gttgatttcg acggttggta atcatccccc catcaaggtc gcctttgtcg
    16321 taattttgct gtttttttcc ttttacttac gaaaaaaaga tcaacgaacg accgatcttg
    16381 agaaaaagta cattgtctcc aaagatactc attgggaaaa acaacaagag ttatcggaac
    16441 aaaataaagc attggccctg tcacaagaaa cacaattagc cttgcaaatt gtggaagaac
    16501 ggaaccgaat tgcccgagac atccatgaca atgtcggtca tctgctgtca agtgcgattt
    16561 tacaaattgg tgcgcttgaa atgatcaatc aagaaccaaa actaaaagaa ccattagaaa
    16621 atttaagtgc caccgttcac actgggatgg atcggattcg tcaaagtgtc catgacctcc
    16681 atgagacatc gatctctttt caacactcct tagagtttct gatcgctgat tttccttctt
    16741 ctgttacgat tgaaggaaac ttgtttgaag gattgaatga aacgcagcaa aactgcttct
    16801 taatggtcat caaagaagcc ttaaccaaca gtatcaaaca tagtcatgcc aaaaccgttg
    16861 ttttacattt tcgaacgttg ccggcctttt atcgcttgca aatcacgaat gatggcacca
    16921 gcccacaaaa aaattctgct cccggtattg gtttgaaaac gatgcgtcaa cgtgttcaag
    16981 aacaaaaggg acaagtccat attttccaag aaaaccagca atttcagtta acgatcattt
    17041 taccaaggga gggacaccaa tgataacagt agtcattatt gatgatgatc catttgtgac
    17101 gaaatcgtta caaaccattt tagaaagcac gaaagagatc cgtgtgttag gcattggtca
    17161 ttgtgccaag gatgcgctag tcttatacga aacccatcgt cccgatgttt tgctgacgga
    17221 catccgcatg cctaaacaaa cgggcatcga tgctgccaaa gaaattttgg ctaaatttcc
    17281 caaagccatc cttttgcttt tgacgacatt caaagatgaa gaatacatcc gcgaagcatt
    17341 ttccatcggt gtcaaagggt atttaatcaa acagaacttg caagcgatta ttccttctgt
    17401 caaatccgct tataatggac aggccgtatt tggtaatgaa atcatcgaga ctttcactca
    17461 aatgatgaag aataaaccga cgattgatag tcatgcttat gcttctttta gtgaacgtga
    17521 gcttgcaatc atcaaagaag tagctgccgg gaaaaacaat aaagaaattg cagatgcctt
    17581 gtacttgagt gacggcactg ttcgcaatta catcagccaa ttactagaaa agctagattt
    17641 acgggatcgc acgcaattgg ctatctattt ttatcagcat ttgcaataag tcttatctaa
    17701 cacaaaaaaa cctgaatgat gctccttcgc atcattcagg tttttttcac atcttctatt
    17761 caattgtccc gcaaaccatt tcctcacgat tgaaagaatc aaggattcgc agggctgcca
    17821 ggattcgttg gattgttcgg cgtgttaggc ggaaaaatag gcgccttgtc taacgttttt
    17881 tctagttttg attcttcacg atcgtctcgt tctacatcag gactttcatt caatttgaat
    17941 tctttttcca tctcatctaa ctttttaaag tcattgccag attcttttgc taatttcgct
    18001 aatttatctt cggtttcttt agccataaag taacgccccc cttttgattt cagcttagca
    18061 tatcacactg aaaaaacaaa agaaaacgct aaccaacgat catttttagc tatttctcat
    18121 ctttaaaatc aagcattttt tgccggtata aaatcgtagg gagaactgtt ttccatacca
    18181 atcatcggat ctactaactg gttatcgatc acttctgctg taagaacgaa atctttagaa
    18241 acagcaggtt ctacctcttc tttcttttct tctccaacaa atgaagaaaa cagatctggt
    18301 tccactgttt ccattacttg gtttttcgtt tcattcgtcc cactgtcatc ttctacaggt
    18361 agcttttctt ttacaacccg tggctctttt gtatagttcc catcttcaaa tggatcattt
    18421 ccgaccaaag aatcttcata agctgctagt ttggtccgga gagtgatatt cattttatca
    18481 ttatccatga tccttgattt caacgtagtc atccggcttg ctgattcatt cgctacacaa
    18541 gtgacatagg cttgctcttc ccctaacaaa atcaacagtt ccttactgcg agtcactgcc
    18601 gtgtagagca aattacgctg aagcatccgc tgatattgat ggaccatggg caaaataacc
    18661 atcttgaatt cactgccttg tgatttatgg attgagcaac aataagaaag agtgatttta
    18721 ttccattcgt ttcttttata agtgacctca tttgcatcaa actggataac taattcatcc
    18781 actttatctt cagaatcttt tgccaagatg atacccgtga tttctcccat atctccattg
    18841 aatacattca attcgggagt attgactaat tggaggactt tgtctcctat ccgataaacg
    18901 gtgtcgttcc aatgtacttc cttttttcca ggtttccctg gattgaatat ctcctgcatc
    18961 attttattta acgcatcgat ccctgcaggt ccccgataca ttggtgctaa tacttggata
    19021 tcttgtgcac ggaatccctt atttttcgct ttttccacaa ttttagagat caagggttca
    19081 atttgataag catcacttcg gaaataggaa cgatcttttt gattctttgt gaaatctttt
    19141 ggcagacgtc cttctttgat ctcatgggca agaggaataa tactagaacc atcaccttgc
    19201 cgataaatct gattgagttc cattttggga atttgttcga tttccaaaag atcatggagt
    19261 acttgaccag gaccgacaga gggaagctga tctttgtctc ctactaagat aacttgcata
    19321 ttcgtcggaa tcgctttgaa taatgtgtta gccagccatg tatcgaccat agacatctca
    19381 tcaacaatca aaagtccgcc ttctaattct ttcgtactga ctgaaggtgt tttttcccgt
    19441 ccattcagcc ccagaagacg atggatcgta ctggcaggca gacctgtcgt ttcgttcatt
    19501 cgtttagctg ctcggccagt tggtgctgca agtagaatag gaaaaatttc ttgggtatat
    19561 tttgaaggat ccaattccag tccgttcaac tctgcaaaaa gattgacgat tccgttgatc
    19621 acagtagttt tccccgtacc tggtcctcct gttaaaataa atagagggga acggattgct
    19681 tcagcgatag cttgttcttg cgaaggtcca tactgtatgc cgaatatttt ttcgatacgc
    19741 cgaatattct tctgcacttc tttttcagga taatggattt cttttttacg actgagaagg
    19801 cgctggatcg aattaccgat cccccattca gaaaagtaca aactgttttc gtaaagatta
    19861 gtctcttctt gttgtatctt tccttcttca acaagttgga taattacagt agcaacctga
    19921 tcaggctgga tttcaattgg gcgacttgtt tctaaaatac ggatcgtttg ctgaagcaat
    19981 gtctccgccg gtacgtatgt atctcctgtt tgaactgcat gctgaaaaat ctgatgcaag
    20041 atagctgctc gaattcttct cggtgaatct gctgcaatac cgatttgttc ggctagatta
    20101 tctgcacgtt tgaacccgat tccttctatg tcttcgacta gctggtacgg attttcttcg
    20161 atgatatcca aagactcgtt cttataggcc tggtagatac tgaaagctaa ctgactaccg
    20221 aacccgtaac gattcagtcc catgatcact tgatccatcc cgtggtttag ccgaatcgtc
    20281 tcagcaagca tttctctttt tggcttcgtc aaaccggaga tttgttttaa taccgaagga
    20341 tcagcaatga ttttttcgat agcatcttct cccagcaatt ctacaatttt ttctgctgtt
    20401 ttcttcccga ttcctgggaa tttttcgctg gaaagatacg ctacgatccc atttgcagtt
    20461 gttggctttt cttgctcata tctttcgagt ttaagctgcc ttccgtattt aggatgatcc
    20521 actaattctc cataaaaacg ataagtctca ttttcttgaa tatcgccgaa acttccggtg
    20581 actacaatct ctttttcacg gtaatcagct gtggtttcac tgatgtgtac caaaagcact
    20641 ttgtaaaaat tactaggatt ttgaaaaaaa atcgcggcaa ctgtaccaac gaaataaggc
    20701 atttcttctg gctgcatcgg aagccgcctc cttcttctat agtaaaggtt ctggtgctgt
    20761 ttcagttcct aaaaaatctg tttggttcca tactttcagt tcatatggca ataatctgca
    20821 aggctcttca gtctctaaaa tcgtcaaact actattgaac aatccaccca tttcacgcaa
    20881 ctcacttagc tcttttcctg tcatccattg aatggctgca gttaaagaag ctccatgtcc
    20941 tacgaataaa agtgggccat ctttatgtcg ggcaaccgca tctgctacaa cagtttcgat
    21001 acgtgtgatt gccgattcaa tcgtttctcc gtgaaaagga gaaggatcat acagatctaa
    21061 atgattacgc aaattcggca gattatccgg atatttctgg tacatttcat cgatcgactg
    21121 accttctaaa gtacccagcc ctagttctct tagcttatct gtataaaaaa tctctaccgg
    21181 acgagcaagc tctttgttga taccttctgc tgtatcacgt gctcgctttg ctgtactgga
    21241 ataaattgct ttaaaaggaa catattttac ctttttccca aatgctttga tttcttcgta
    21301 actttgtggc aaaagcggag aatcgccatt gctcccttga aaacgtcttt cctgattcca
    21361 ttgtgtcttc ccatgtcggg taaaatatag ttccatacgc agcaaactcc tgtctttctt
    21421 ttattttact ctataataaa ctgtctaatc cagcttgtca ttttcatagt gatgcgtcaa
    21481 ttctaaatta gggaatcctt gctggcgaag tgcttcatac actatcattg cagctgtatt
    21541 cgataaattc aatgagcgga catgctcatc attcataggg atgcgtaaac acttttcttc
    21601 gttttcacgc ataaatgttt ctggcaaacc ggttgtttct tttccaaata aaaagaagtg
    21661 ctcttctcca tcagcaaaat tttcgtcact atatacttta tgggcaaatt ttgagatcaa
    21721 atgtagttta cgattaccta aaaatgttaa aaaagcttct aaattactgt ggtaagtaat
    21781 atttacatca ttccaataat ccaaccctgc ccgttttaga tgtttgtcat cagttgaaaa
    21841 acctagtggc tcgattaaat gaagcggagt attcgtggct gcacacgtac gggcaatatt
    21901 tccagtgttt gcagggatta atggttcaaa taaaacgata tgattcatca ggatcattcc
    21961 tctcatattt atgttcgttt ctattttctg acaatttgat ttttgcataa aaaaaaacgt
    22021 atcaactcgg tgtcagtcac tgcgagtcgt tacgttagtg aagctcttct cactaacttt
    22081 tatcagaaca ggaacctgat aaaaaccaat gaaaaaacaa cttgttgcta atataacacc
    22141 ctcaactttt ttttgcaacc tctactcatt aaaaactgct agaaaaacgt tttctcttag
    22201 ttccttttaa taattctatg tcaattgtaa tcttcttcaa aggattctta tataagtctg
    22261 ctttgattcg ctcttctacc tgccattcta aaggtgacat ggccaaaaga tctttttgat
    22321 tttcctctgg tatctcaaat tcataggtta cacgctgacg ttcagttaat ttcattgctt
    22381 cagcaaactt ggctactaca cgttcattgg aataactctg tttttcaggt tgattcggat
    22441 aaaaagcctg acgtagttcc tttaaataat tttcttgtgg caccaccttc aacacttctc
    22501 catcttctga gagtacacgt tggaattctt ggtaatgaga aggcgaaaag atatttaaaa
    22561 ccgcgttgaa tgtttgatca gcaaacggca aattggtcaa atcagctaca caccaaaatg
    22621 cttcggcaga ttgttctgta gctgcgtaga ctccttcttt tgaaatatca aatccaacag
    22681 cattttgaat ctttttcaat tttgaaagtt cattcaagaa gctaccttct ccgcagccaa
    22741 catctaatag cgagacttct tcaggtaaat gctgcgcaat agtctcaata acgggctgat
    22801 acatgccaga ttggatcatt ctacgccttg gaacgaacat tccttgatcg tattctgttt
    22861 taaccgcatg ggataaaaaa taaagtgttc cttttttgga aaggtcaaaa cggtgattat
    22921 ttttgcatat caagccatta tcagactttt ttacttcttc ttggcaaacc ggacagcgga
    22981 acagctgatg attttctgcc aaaaactggc gtcctcggtc aatttttttt aacatagttc
    23041 cccctcattt ctgttttatc ttaccgttag atgctcactt tggcaactgc taaaaatatg
    23101 atacaatgcc tagaagaaag tgaaagataa aggagaagat tagatgatca aagaattttg
    23161 cgctgaaaac tacacatcta ttcctctagc aattgctaga ggagccgatc ggatcgaatt
    23221 gtgtgacaac ttagcggtcg gtggcacaac cccgagtact ggtgtgatcg aagaaacgtt
    23281 aagttatgct ggggaaaaag atatccccgt aatgacgatt attcgtccta gagggggcga
    23341 cttcgtctat aacgatatcg aattgaaaat catgcatact gatttgattg aagcaaagaa
    23401 attagggacg gatggcgtag tatttggttg tcttactcct tcaggctggc tagacgaaga
    23461 ggccttagaa ctattgatcg aaacagcaga agggttgcag atcaccttcc atatggcttt
    23521 tgacagtatt cctgaagatc ggcaattcga agcaatcgat tggctggtag aacatggtgt
    23581 tgaccgtatc ttgactcatg gcggtcctgc tgacaaaact attgtagaaa atttcccacg
    23641 gcttaaagaa ttgattgcct atacagatgg ccgcttaatc attttgcctg gtggtggtgt
    23701 cactgcagag aatgcagcac atgtagctaa agaattgaac gtaagcgaag tacacggaac
    23761 caaaatcgtg gatcttcagt tataatctta taaacaaaaa aacatttgca acaggcataa
    23821 tgcttattgc aaatgttttt gtttttttac ataaaatagc ccataaataa agaagcaata
    23881 ttgaaataaa tcagtacact cgtcaaatcg ctcaaagtcg tgataaaggg accgcttgct
    23941 actgctggat caaatcctag acgatccatt agcatcggga ttaaactacc tgcaagattc
    24001 gccactgtga ttgcaaatag catagccatc cctatgacaa accccaagat aaaattatgt
    24061 ttccaaaaac ctacgattag aaaaatcgtg atccccgtaa cagctcctgt gacaacgccg
    24121 gtcaagactt cagccaaaac aagtcgaaca aatccatgat ctttctcatc agaaatcgca
    24181 agtcgacgaa cagccacagc tagcgactgg gttccagcat taccggcagt tcctgtaatc
    24241 agcgaaatga agactgctaa aatactcgct tcacttacca agtcttcata gtgactgatt
    24301 aaagaagcgg tcgacatacc taaaaacaac agagtgatca gccaaggcaa acgtcgagac
    24361 gctgccttta ctggattttc attaatttct tccacattga ccccggcaag tcctgaataa
    24421 tcgctagcag cttcatcatc aataacgtcg atgatatcat cgactgtgac gattcctaac
    24481 aaatgatcat cataatcggt aacaggaaga gctaagaagt cataatctct aaatgtttgt
    24541 gccacatctt cttggtcgtc gcctacatga actgaaagca ctcgttcgct catcagatca
    24601 gaaatcattg cgtcgtcatc attcacgatt aggtcgcgca gagaaataac ccctactagc
    24661 tgaccttctt gattaacgac atagatgtaa tagattgttt cagctacatc tgcttcgttc
    24721 ttcagcacgt acatcgcaga gcgaacagtt tgattggcta cgatcgaaac aaattcagtt
    24781 gtcatgatcg cgccagccgt ttcatcctcg taatggagaa gttccttgat ttcactggca
    24841 tcgtctgccg acatcaaact taaatattta gcaatttgct ttttatccag cgtattaagc
    24901 aagtcaacag cattatctgt atacatctca gaaagcatat cagctgcata gctaggacgc
    24961 atttcatcta ggtattcttt catatgttcg ttgtcttctt caatcacatc gaacatatcc
    25021 gctaattctt taggcgacag ataggaatag atcagctgcc tgttctcttc tgtaagagat
    25081 tggtagaatt gcccctgctc atagatatgc atctctaaaa aactgtttct gaatgcctga
    25141 atattatttt cccttaaatc ttgagtcaac tgggcaaatc tttcctctaa ttcttgattt
    25201 tcatccaaac caatctcacc tcgccttatt cttcaaaccg tctcacaact gcctccatgt
    25261 ctgcggctaa aggctgcttg aactccagca actcttctgt aaatggatgg taaaaacgca
    25321 gatcataaca atgaagggct tgtcttttta ttcccagatc cattcgcccg ccatacatat
    25381 catcaccgag cagggcacag ccaattgcgg aaaaatgaac tcttatttga tgtgtccttc
    25441 ctgtatgcag ctggatatca actaacgcga tttctgttag tcgcttctct atccagtact
    25501 cagtctgtgc cgaacgtcct tcatcagaga ccattcgctt caatagcgaa gaataatctc
    25561 gggcgatcgg caaatcgata tgtccatgtg ccgataaatc tgaaagcctt ccactgacta
    25621 aggcctgata ttttttcaca aacttctttt cacgaagttg catatctaat tttgcgtgcg
    25681 caaatccatg ctttgcaaaa agcatcaagc cagatgtatc ccgatctagt cgcgtcacca
    25741 catggattac ttggttttcg taaccttgtt ttttataata agcctttaca cgattagcca
    25801 ttgtcccatt tggatggtat tgggcaggaa tagacgaaac gcctgctggt ttatttacta
    25861 ccagaacgtg ttcatcttca tacacgatat cgatcggtgt ttcatccaaa agtacggttt
    25921 catgttctcc ttcagcagga atcacaatgg ttaccttatc atccttagct agagaaaaca
    25981 agacattttc tacttgatca ttaactttga tttgaccgcc ttgaaatttt attttcgcta
    26041 acagaccctt tgagatccct ttttctttta aaaaatattt tacttgctga gaatgatctt
    26101 tctcataatc ccatgtaaac tgcatatcaa tcctcactaa taaaggcatc tttcacacga
    26161 tgccaaaaat gcatatgtcg ataagaagca aaatggatcc gttcttctgc tattcgataa
    26221 taaattgagc ggatatttgc ttttgcgaca ttcaattgat caaccgttac aagataatct
    26281 tctgtatttt ccaattttat ttctagccat tcatcatggg caatcacgat cggagaaccc
    26341 aaagtacgaa acactcgatt attcagtgaa gcaatctctg ccaactgaaa ggcattgata
    26401 ctaggatgga tcacagcacc gccgacactt ttattatatg ctgtcgaacc agtaggcgta
    26461 gaaatcgtca atccatctcc tctaaaactt tcaaacagct cgtttttgat gtatacatcg
    26521 gctaccatcg tacgattggc ccgtttaatc gttgattcat tcagtgctaa aaaatgctgg
    26581 tccggtgttt catccaaata actgatccga acatccagca agggataact aacactttgt
    26641 tcccggttcg ttcgtaagct gtctactagt tcttccagct cgtaatcccg ccaatccgtg
    26701 taaaacccta aatgacctgt atgcacgccc aagaaacgca catcattcag acaatgactg
    26761 tagtgatgga acgccgacaa taatgtgccg tctccgccga ctgagatgac caattctggc
    26821 tcatgatcat ctcgttgaat ccctgcttgt tctaaaagtg cgttcaaacg ttctgtcact
    26881 tctatagtct ttgcttcttt attatgaacg atcgctactt tcatcccctg cctcctctct
    26941 ttcgctttct ttcttcaaaa gcgttcgata gtagacatca tctgattttc ctcttccatg
    27001 ggagaataac cgctgtgctt cttggatttc ttcacggata ctggacatct cttcatccaa
    27061 ctgataggca gcttctgccg cacgctgaag tcgttcactc atttcttctg gaaaaacgcc
    27121 ttgatattta taattcagcg aatgctcaat cgttgcccag aaattcatcg caagagtccg
    27181 aatctggatt tccgccaaaa ttttcttctc accgctgatc agctgtacag gatactcgat
    27241 caccagatga taagaacggt agccacttgc ttttttattt gtgatgtaat ctcgttcttg
    27301 gagaactttc atgtcttttc ttttacggat gatctcaaca acttgatgga tatcttctac
    27361 aaactgacac atgatcctta acccggcaat atcagacatt tcttcttcta aacgtcccat
    27421 tggaatatgc cgtaattgtg attttgttac aatactatcg actggcttga ctctcccagt
    27481 gacaaattca atcgggacat gtcgattttg ttcacggaac tgtttgcgga tcccccgcag
    27541 tttcactttc aattctgata cagtttgttc atatggtgct aaaaatgctt cccaatctcg
    27601 ttccatagct gatacctcta cttcattaac attcattgtt tattctacca tatatgcaaa
    27661 gttttttcgt actcttgatt tatgcattga ccaaaaaaag aaaattaggc aaaatagaca
    27721 aaaaggagtc gatcaaatga cacaaatgct tgaaatcgaa tataaatcta tgctgacaaa
    27781 gcaagagtac gagatattca tcgctcacta tcaattaaca gataaagatt tccgtgtaca
    27841 gactaatatt tatttcgata ccgttgatga acagctgaag aaacaaaatt gtggcctacg
    27901 gatccgtttc gttggtcatc aagctgaata tacattaaaa acacctgctg aaaaagggcg
    27961 tctcgaaaca acggatacct ttataacaga agaagcagaa gcatttatct tcgaaaaacg
    28021 tctgcctcgt tcaggcgcag tttttcaaaa actttctgat ttgaatatcg atcctgcttc
    28081 attaatccaa accggaaagc ttacaacaaa acgagctgaa ttccctattg aagaaggact
    28141 tttagctatt gatgaaagct ggggagacaa tctacacgat tttgaactgg aactagaggt
    28201 aggggatgca tctgtgggaa aaattgcttt tataaatttt ttaaaacgtt tttctttacc
    28261 ttatcgtcct gctaagaaca agatccaacg tatgcttgaa gctaaaaatt agaaaccttt
    28321 cattttcaca aggcacttga gttcattttt ttactttttt ctgttaagtt ataggatgtg
    28381 aaagaatcta gtcactagtt gtatagaatc tattttgggg gagattcata tgatcgaaat
    28441 ctatttattt gttaatcctt taggggaata ctgtttcgaa atggaacaac agttattaca
    28501 atttatcgaa gaagagtacg ggacaccatc aaaagaaaag atgcaatttc gctttcttcc
    28561 tttggtcaat ttgcagacaa ttggggatgt catgcaacaa aaaggaattt ctcagaacaa
    28621 tcttgaggaa cgtaatcact tattctcaat gacttattct gcagcattgg actgcaaagc
    28681 ggcgcagttt caaggaaaga aaaaaggccg ccgttttttg atcaaattgc aagaagcagt
    28741 cggctgtaat gacataccat attctcaaga acttgctgaa tccatatttg aagaaatcgg
    28801 cggcgatttg aatatgttta aagaagatcg tcagtctgac ttcgttaaag aagtattctt
    28861 ttctgatcaa aaaattgctc gggaaatggg catcacaaaa cacccttctg cagtcgttta
    28921 caattatgcc tgcgaccgtg attttggtgt tttagtagaa gtaaaaggta caagtagcct
    28981 ttggagaatc acagagctgt gtaaaacaac agataactca tatcaagttt ttaatggtga
    29041 acatttacat acaaatgatt ctcagttcca ttcaagaact agccatttac acttgctttc
    29101 taattaaaa
//
LOCUS       DACKAC010000034        27993 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.034, whole genome shotgun sequence.
ACCESSION   DACKAC010000034 DACKAC010000000
VERSION     DACKAC010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.034"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(1153..1551)
                     /locus_tag="I0B46_001520"
     CDS             complement(1153..1551)
                     /locus_tag="I0B46_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083829.1"
                     /translation="MRLRRNKNYTTIILEDFTDQELLIVGKTKADFIEKGNASIVKAS
                     KIAVEKYFDSLEESAKEIFNGEKAGSYELDGIRFSLIPTTENIIPKKLYYIFSSEKHT
                     SMKPCIFDWQWLIDAVLDVKCSKYNITLGC"
     gene            1767..2822
                     /locus_tag="I0B46_001521"
     CDS             1767..2822
                     /locus_tag="I0B46_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HAR2083830.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYQNI
                     LSFSLANLARLEDQSDDVLTANLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDETLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVVEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            2842..3195
                     /locus_tag="I0B46_001522"
     CDS             2842..3195
                     /locus_tag="I0B46_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083831.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDRNDSKVAEVKNKPTDHNPIQQEHPSTVTSDHSAKKEK
                     PMLKGFNAPSNKDLL"
     gene            complement(3377..3673)
                     /locus_tag="I0B46_001523"
     CDS             complement(3377..3673)
                     /locus_tag="I0B46_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083832.1"
                     /translation="MIEVKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETTSSLVAK
                     LKSDSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(3677..4495)
                     /locus_tag="I0B46_001524"
     CDS             complement(3677..4495)
                     /locus_tag="I0B46_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAR2083833.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAVNENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWMVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
     gene            4721..4942
                     /locus_tag="I0B46_001525"
     CDS             4721..4942
                     /locus_tag="I0B46_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HAR2083834.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKELMKEHEENDQSISHEDMLKELGL"
     gene            4944..5210
                     /locus_tag="I0B46_001526"
     CDS             4944..5210
                     /locus_tag="I0B46_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAR2083835.1"
                     /translation="MTYQVRYEREAQKALKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            5323..5862
                     /locus_tag="I0B46_001527"
     CDS             5323..5862
                     /locus_tag="I0B46_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083836.1"
                     /translation="MYNISVNKLINFQKERFFMGWYGAYSSANEVIEEKKGYAKNGLE
                     IVDTKVTKTYGIILFKDAEGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFF
                     VGDQEKMVKQQLDRIKEENNKPNLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQ
                     DGSLTRFTRLSAGDVKEVA"
     gene            6356..6640
                     /locus_tag="I0B46_001528"
     CDS             6356..6640
                     /locus_tag="I0B46_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083837.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGVVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKNSFIGKLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            6914..7168
                     /locus_tag="I0B46_001529"
     CDS             6914..7168
                     /locus_tag="I0B46_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083838.1"
                     /translation="MNYTDEEQITLEYVFTNEKGEEIEKVLIISSKDLENALTYEPYE
                     STVTVNEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            7250..7435
                     /locus_tag="I0B46_001530"
     CDS             7250..7435
                     /locus_tag="I0B46_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HAR2083839.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            7502..7897
                     /locus_tag="I0B46_001531"
     CDS             7502..7897
                     /locus_tag="I0B46_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAR2083840.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELFEK"
     gene            7887..8264
                     /locus_tag="I0B46_001532"
     CDS             7887..8264
                     /locus_tag="I0B46_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083841.1"
                     /translation="MKSKKETEPMKATHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            8265..8661
                     /locus_tag="I0B46_001533"
                     /pseudo
     CDS             8265..8661
                     /locus_tag="I0B46_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350537.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8967..9365)
                     /locus_tag="I0B46_001534"
     CDS             complement(8967..9365)
                     /locus_tag="I0B46_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAR2083842.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
     gene            complement(9556..10323)
                     /locus_tag="I0B46_001535"
     CDS             complement(9556..10323)
                     /locus_tag="I0B46_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAR2083843.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPNKLKKT
                     GEIVLPVKAIIKYMDVDQNLETELKNIVNTLQRQAVYEISNLDKEYKTYATVSAIDQI
                     HWDLEEQIITFNFSQEVIPLILSIKKKYPYFVFQDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LKIDFAKVRKGRNYNFAVQFNISSEFDEKPYREKKLG"
     gene            complement(10358..11581)
                     /locus_tag="I0B46_001536"
     CDS             complement(10358..11581)
                     /locus_tag="I0B46_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAR2083844.1"
                     /translation="MESYQFQVFKLVPSLERRGNRMKITEKNVNPKFETTESNLKKFD
                     LTVTLSNQLVDSIVEMDAMPQKIYLYAIGHLDPKNPPFNNTVKVSKKALFALMSSSSS
                     SSKHSRLKQVIEKLHQQSVIKIQRTKPNGKTEYKVISPLEITEFNDFSDAVSIKFSSS
                     IMPLLTNLEDHFTQYTFDQVVNLTSKYAISFFQLFTKDFNLYEANRDNLTWSTKSLDR
                     LKNPVFSIADLRKKFSIEKKYKAFRDFERRVIKEPIAQLNKYTDFKIDYELIKKGHTY
                     TNIKFLLTRTMSVPVPYKQEQRDPAYLKGAEDKTEKMNQSYLKAMNSKYTKLLIKNFL
                     ISSADLCDLKLMSRLQNKVYPLYDRLSKDRGFESVVNHLSYVATHKEDYTIKNTVKYL
                     EKSITSYLPYVNLYD"
     gene            12562..13365
                     /locus_tag="I0B46_001537"
     CDS             12562..13365
                     /locus_tag="I0B46_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAR2083845.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKDLSDKLGNLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
     gene            13950..14405
                     /locus_tag="I0B46_001538"
     CDS             13950..14405
                     /locus_tag="I0B46_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083846.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            14524..15582
                     /locus_tag="I0B46_001539"
     CDS             14524..15582
                     /locus_tag="I0B46_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HAR2083847.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLDERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVSKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            15624..17573
                     /locus_tag="I0B46_001540"
     CDS             15624..17573
                     /locus_tag="I0B46_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAR2083848.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKSEDKENEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQL
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFK
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            17576..21217
                     /locus_tag="I0B46_001541"
     CDS             17576..21217
                     /locus_tag="I0B46_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083849.1"
                     /translation="MKFKKYLLQNLRIKRWVVRGLVIGGVLAVTLGAPFGMNDPTPDN
                     QDSKQETVINKNVAYAESKPAEQGMWTLYARILMGDKEVNDSAAKTGQMNLLGQGGIS
                     ADFPYSDISKQGNSIAGGNAGTQLASTLATYSYYGYIETVSGNAIASQASSILGSIGR
                     TIGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGEGGSDVSNPVSKALTSLL
                     NNLGLNNELFKAIASLSMLFFTAILGYQGMKLLSKDGVNSKGLSPVLEKWIVRIFVLF
                     IFLPVAGSLLGVYINGIQSAKKDGDIPTNVVSDYIINTRLWASTQNLAPSSDTGGDVP
                     DGKPTSEIKFVDTDYEPSKKSAMIRAINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NINTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGSAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTAHNFDATQPWGVANNATFSTQSVALLLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFIRIWEVVLTGSLASGAGVMAISLGVVTTSLIGLQLPIFLITFIRAFGDTAV
                     GFIPNTLVGDGVANIVRSAMILGVGYLLVFKKGEKVNNLINMLIGLPLEVAFGFDERA
                     KLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGNSSVKDMVAGGLGAVGAQKMAER
                     LNNMGDSNADAKSIADKVIPETPEGMSFNGGGVGSTINNTSFKDLVRQDQLRNNSQQY
                     IDHLGRMVDENGNFVDENGEILKDSKYGKDAKGNFIDEKGRLVDEDGNLIDTNGKPLE
                     DSEDGNIGMDSEGRLIDEKGRLVDEDGNLVDENGRRLLNGDFGHDSNGRLVDEKGRLV
                     DEDGNLVDEFGNKLGKHSDGYNVNGDLVDEDGNIIETRDALVADRNRELEQQRHTPIA
                     VAIPENEEDEITLDQTHQELRNEMSGEDVSDRVHRELGDGASGESQSRELNSNFDNSA
                     IRNDTVLKDRESLTKETDKEQSDVKKKFIPTPNVNQTDKYETNIGSGMSKDNQSIREP
                     ELTNKNNRTNNSKSTSDNSGKSNQSTKKTSTAKSAVEYLSKTGKNVYTSADKALAKAP
                     KGSLAGITYEAKNEAIKIGKGTAKVAKATATGASKTGKVVKKSTMSVKDYIVKYGNQK
                     STENKSEDE"
     gene            21617..21955
                     /locus_tag="I0B46_001542"
     CDS             21617..21955
                     /locus_tag="I0B46_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083850.1"
                     /translation="MFDSIKNLVSGMTPDMKTLVQVVLYFLAFVFSLKPGFTAMKFFG
                     DAKWGDGLKYVGAIIAIFIIPVITTVMLYTMGTKTGNDLNQKANIISALIPVVGIYLV
                     SKFPKNELLG"
     gene            22065..22286
                     /locus_tag="I0B46_001543"
     CDS             22065..22286
                     /locus_tag="I0B46_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083851.1"
                     /translation="MVFDREKMLAHANEVLMSSLKGTELAKIMNMNVNQFYDYRNGSK
                     KIEKARLETLIKFEKAYVYMLDKQKRTID"
     gene            22306..24462
                     /locus_tag="I0B46_001544"
     CDS             22306..24462
                     /locus_tag="I0B46_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083852.1"
                     /translation="MAKFKDITESINYVAETVPVVALSLKPQLLEVYKKEEKEAKAKK
                     AKAKVWKEFDALLTKDLPKKNYMVIEDVQKLNSEGVIDEDDAILDSEKYVVLLKSSLN
                     YVADLPEKFKEKLLAGVRFMDSSGQLLVIPDDPNVFESEVEEATDFYSAVEQLEVATN
                     GLERIAETFNSSEEVEFEAVAENVPDEKEALFVDDNKPTEAENQEVSIEETQIQTETY
                     QQPKIDEKPESQSDKENYVYEAEKITSESDVPKNLDADFLEKNFEQIIADKFQPVVLD
                     EINDPVDLNEEMLSIPELKPLLFLAKESMNARISIANNKINSELNKTKEQLILKGKQM
                     LADELLRIEEATSLNGQNQFTNTLSVAKESYEAEASKIDDLVMEKSNIIRKDYDMAKQ
                     AYLDAELKKLEQRYDAEHLPEVKQQEDEYRKATEESLDQQYQEAIESIKNDALKQSQE
                     AKEVVVERIINSLKPEIEKSLTDVQEKTLEIVEKTKSENEAEFHDTKEKTTDVVVKYF
                     ANKEEVEKQVANNTKELETKLVEQQKTIEEYKDRMKEVKDLAEGYKLNSEKLAEQSEA
                     LREDLSRSRSDLAQMAEKTKSLAVATENQSSSKRDFTSKKMATIISVAAMACGTILGS
                     SYFISHNNQQAQAQTQTSISRVENSIGTNTNSASTNSSYEATPTTEFKVGKDVVISID
                     GKTTKAEVISIDGEKAVVKTPDGNQYKIQAHLSDNN"
     gene            24474..24707
                     /locus_tag="I0B46_001545"
     CDS             24474..24707
                     /locus_tag="I0B46_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAR2083853.1"
                     /translation="MREEKIVYPVIQNNQENMTSSQHLQEAQLVYQVNELPQTNETMS
                     KSISLIGLLIVAIVITVGVYRKLNVKGSISDGR"
     gene            24697..25563
                     /locus_tag="I0B46_001546"
     CDS             24697..25563
                     /locus_tag="I0B46_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083854.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVEWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLKFIFVPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNSARVSMGKKKPFRGTEILKVKQDGTIVYRDGSVALMFNLDGATSLTAFP
                     EEVMAQESKSIQYQNTRDRSLAEIKITSSQPQNTERQTRNMKIQKRNARSNKAVFDII
                     DQQERFVSEYIDGVKTTVIQYLILKSQSKMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSDIYALK"
     gene            25578..27548
                     /locus_tag="I0B46_001547"
     CDS             25578..27548
                     /locus_tag="I0B46_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083855.1"
                     /translation="MEIMGRSQLLNFKKKRKTKKEQKTRNSKVVNNTFVSDLTSLVGY
                     QFTPSYIRTGKRWGTIVKLVNTYGMNRNYGFGWMVKAIPEVRVEGVKAYLIEADKPVS
                     QKDQQEIMHENVGGSTNSYENNLEGKNSDAEISKTVMRIMDLNTASLLDSMANKIIDW
                     KLEILLVSDSPNKISEQLIKLQRLYDEKMYGLKLMSYGGDQRDLFDRLLSEPIGNVYE
                     YTSMSSDFAGNDHAIRKGLDDANGWAIGTMSTSYASGVAFMALDDSFTSKVLVASHKN
                     ATIKGYDEKISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGRTFVAKPSLNS
                     VIEKVNLAEEGLNSLELFGNQKDIGDLYRRNLDKLTQIFYLLAGRTLDSATRVLLKKE
                     LGNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNAETKAKKSGTEKEVDRI
                     IVLKDTLRTALDSFSRMFNQRTTLKEIGKSDKLQFYYNLGDLRAETNVMEAQFLNTFD
                     FITSQAKKDDIVMIHGFDKLSIETVGLIKKRLTMLEEDGIRLAFLFDTIGSGEDNEYK
                     EDIETADIFNTDGKIYQSFESDFNYTIIGKMTSGELMKYEKKVAPARKGEDDQKLSVD
                     LRQYLTTGRSNKFQIRRPVDLTNNFVIGEFIL"
     gene            27715..27942
                     /locus_tag="I0B46_001548"
     CDS             27715..27942
                     /locus_tag="I0B46_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083856.1"
                     /translation="MKPNIKILDRIFLGRDTEVILIQHEEGFEVSIGIQKLQKPHYCN
                     QLYKNFTDEEKARVFFNEVKGMREQYEVVEA"
BASE COUNT        10030 a         4253 c         5044 g         8666 t
ORIGIN      
        1 acttttgttt cgtgttaagt ttctttacat tcctacgcac ctcgagtgta aaatttcttt
       61 attcctccca cgctccatgt tattttatat ttcctttttc atgttatttt tatccagaaa
      121 ccgatcaagt ttttacctat tcccaaaatc catttattcg ttattttttt cactgattaa
      181 gaataattat ttgatgctta aattagctaa aaaagcgatt tgttactgat ataatcatga
      241 tattgcagct gaattaggcg gaaagataaa atgttcgcaa aatcaaagta tcaattatct
      301 tgttttttta taaatttgta agtaaaaatc taaaaaagtt ttcttttaat tggtatatgt
      361 attaaaatca catttaatca cccataaagt atgcgctttc atttttttgg atgagattaa
      421 tatcaataaa taacgatgta aaaatcattt attatgctct tttataagga tattaattga
      481 tctaatttga aaaaaaatca aaatttaaat gttttaatat tacaaataaa atcatgttat
      541 tgtaaaatgg tataaaaaag ctaaatattc tatttttgtt attttattag caggaaaatt
      601 taaatagttt tacaagaatc cgtagaaata tattggtttt gccttcagat taatactgga
      661 tcatattaat ctgaatataa tatatgaact aataaatagc ttaacctcgc tattttgttt
      721 tttctgatca ctatctatac agcattagta aatagaattt taaaaataaa aaaattaaga
      781 caaaaaagta tcaacataaa catatacata tacacgttta tgttgattca cttaatttgt
      841 cctttaaaaa gcacttattt taatataatg ttattttata atcttattac aagctgattg
      901 ttaggcgttg tcctttcatt acatttatta acggttattt tattgtttaa tttattaatt
      961 atttttgatc taaaacttag cttaacaaat aaataattaa ccaaccgcca gataaaaatg
     1021 cgatcattgc taatacataa gcaaagataa aaatatgttc gatcagctta aaccctgatg
     1081 cgattaagca gaccaagtaa aatagaaatt ctcttacaga taatggtaat tttctaacta
     1141 gcatactcat aattaacacc ccaaagtaat attgtatttt gaacatttta catccaatac
     1201 agcatcaatc aaccattgcc aatcaaaaat acaaggcttc attgaagtat gtttttcaga
     1261 tgaaaagata taatataatt tcttcggaat aatattttca gttgttggga tcaagctaaa
     1321 tctaataccg tctaactcat agctaccagc tttctctcca ttaaaaattt ccttagcact
     1381 ttcttctaaa gaatcaaaat atttttcgac agcaatcttt gaagctttta ctattgaagc
     1441 atttcccttt tcgataaaat ctgcttttgt tttaccgaca ataagtaatt cttgatcagt
     1501 aaaatcttct aaaataatgg tagtgtaatt cttatttctt ctcaatcgca tcaggatcac
     1561 tccttaatat aatttatgtt tttataatat cataataaga taaatttgca agcatttatt
     1621 tacacctttt tatttacact ttattataat taattagact tttatgattg catttttttc
     1681 ttataaacag ttgcaactaa tagtaaatag gtgtaaacta ttttcgtacc gaaataccat
     1741 aaacacaaac agaaagaggt cataccatga aagaaccatt attgttttcc gttgacttag
     1801 ggaataaatc agtaaaagaa atgagtacac acgtttctcc actctcactt ccctctcgta
     1861 ttttgaacaa aaatatcatt aattcaggac gtttttctaa tataggttcc tcattaaaac
     1921 gatcactaaa cgtttctgaa tatagcctag taaacactac tgaaagctat tactttggga
     1981 aggatataca cgagctagga aaagatgact acgtgataga atcactaggt tttgggatca
     2041 gcagatacaa gcaggaaacc taccaaaata tcctatcttt ctctttagct aacctagcac
     2101 gtttagaaga tcaatctgat gacgttttaa ccgctaatct agttttaggt ctgccttcat
     2161 ccgactatac cgaaacaatc gtcaaatacg tgatgcaaca agccacacga caacattcag
     2221 ttgaagttga cggtaaagct tataatataa acgtagatac cgtttgggta ctgccacagc
     2281 cgattggaac actatataac ctcctacttg ctgatgatgg tagtgtacaa gatgaaacat
     2341 tgatcagtca gaagatcggt tttgttgata ttggcggtgg cacgaaactt tttgatgttg
     2401 tccaaaattt tgaaattgac gaaaacaatc gtgaacaaaa ggataccggc atttttactt
     2461 tatataatcg tatcgtaaat atgcttgacc ttccgatcag tcctaatgct ttccaacttg
     2521 aaaagattat ccgagaaggc aaagaaaaag gcgtatatct atttaagcca agcggaaatg
     2581 aaagttttga tatttcagaa gtagttgaaa gagaaataaa aacatacacc aatatggttg
     2641 ttgcagaagt tttagctaca ttcaaaaact tcacaacctt agataaagtt gtctttactg
     2701 gtggtggagc ttctattatc gacaaacaac ttgtaaaaga taaaattccg cataccttgt
     2761 ttgttgaaga cagtgaattt tctaatgtaa aaggctttta caagtttggt ttaactcaat
     2821 aaaacagcga aggagatcaa catgaataag ggaaaatcag agcgttatgt agttataatc
     2881 aatagagaaa aggttagcga gttaccttta gcagagtttc ttgaatccga atatgaaaca
     2941 ctaggcgtta aatactctca gacgatcaaa aaagctttga acctatatcg gcgtgtaaag
     3001 aacaatgaag ttacaattat tgaagcaggc aacgatagaa acgatagcaa agtagcagaa
     3061 gtaaagaata aaccaactga tcataaccct attcaacaag agcatccttc aacggttaca
     3121 tccgatcatt cagcaaagaa agaaaagcca atgctgaaag ggttcaatgc accaagtaac
     3181 aaagacttac tttaaaaata aaaaggcgag gttacaatac ctagcctttt tattatgtac
     3241 aaatataaac ataaacatat acgtatacat gtatataaac atgtatacgt atatatccat
     3301 aaacacgtat attaaattta gttaagctat acctaaccgc ttattccaat aaaaaaagtg
     3361 atcagcttta aactgatcac tcaccaaaaa cttcatcata caagttatct atcatttcac
     3421 caagtaaagc tgaatctgat cgaattccta acttttcgct gattttagat agtttctttc
     3481 tttgcgatgg ttgaagcgtg aattgataat ttttcttctt ttcagaacta tcacttttta
     3541 gtttagctac caaagaactt gttgtttctt taggtgtttg tttaggttct tcaactgctt
     3601 caaccacttc aggaacttct tctacttcag cttcagcgaa ttttttaaat ctattttctt
     3661 tcacttcaat cactccttat tcttgtgccg tatcaattat tttgttgtat agcgtaaacg
     3721 tgctttcaaa gaaatcctta ttcttacgat agatattctt atccttagcg aactcagcaa
     3781 tattcttacc ttctaagcta gttgcattaa gcaattcctt ctcttgtaca tatccaagat
     3841 aattagataa cgttggtacc agttctagga actcacgact tgaattagta ttatgtttga
     3901 tcctgctacc gatcgtagcg tatttagttt ccatcagtgt ttcaccagtc ttaatatcaa
     3961 tcaactgttt tgctagtgca tcaatatctt taccaagctt cagcaaagct tcaaaaccat
     4021 ctttagatgg atcagaaatg cctacgacaa gatcagatac agcccaagca ttttgagcta
     4081 gtagacctgc atcattatga gtatcaatca gaattagatc atacttctct ttaagctgtt
     4141 catagttaac aaccatccat ctaaacagta gggttctgtt gttaggcata tcacgcattt
     4201 gcatctcaac ttgttttaaa tcttcacctg ctactaaaat atccatgttc tcatttacag
     4261 ctaaaggttc agctgatcca tgaagatata aatgattgat tgtattctct cttttcgctt
     4321 tactgaaagc atcaggaaat cgttttgata ggttttgcga cttatcttca tcaatataca
     4381 gcaccttcat acctaaattt tcgacagcat atccacctag attgtagatt gttgttgttt
     4441 tacctgttcc acctttttca atgtttccag taatgattga caacttcttt tccatgatta
     4501 aacctccctt attaaccttt tgtaactcca ttatacacta taaacacgtt tatgtatatg
     4561 tttatataca taaacgtgtt tatatttata tttgctttta tattctacaa cataaacata
     4621 tatatgtata tgtttatgtt tatgtatata ttcatatata taactattgc atttgtcata
     4681 caatcgtagt acaatgtaat tgaaaaggag ttgataaact atgccagtaa taagtattcg
     4741 tgtatctaaa gaagaaaaag attttcttga aaaagttgct gaattcaacg gagataacat
     4801 ttctgaattt atgcgtagta attccctgca aacagcagaa acgttagtag actttgccac
     4861 ctacaaggaa ctaatgaaag agcatgagga aaacgatcaa agtatttccc acgaagatat
     4921 gctaaaagag ttaggcttat aagatgactt accaagtaag atatgaacgt gaagctcaaa
     4981 aagcgctaaa aaaaatggat aagtttcagg caaaggttat cctaaactgg atcgagaaaa
     5041 atcttgtagg cactgatgat ccaagaaaac atggtaaagg tctaacagct aataaatcag
     5101 gctactggcg atatagggta ggtgcctacc gtataatagc tgatattagc gagcatgaag
     5161 tgacaattct tatattgtct attggtcaca gaagtgaagt ttataagtaa cagacaagct
     5221 gataaggctt gtctgttttt ttgttaaccc aacaaaatca ctgtttacga tcccacttta
     5281 cctaaaaaag aacaataccc tcttgttatt atacacactg gtgtgtataa tataagtgta
     5341 aacaagctaa ttaactttca aaaggagcga ttttttatgg gatggtatgg tgcttattca
     5401 tcagcaaatg aagtgatcga agaaaagaaa gggtacgcca aaaatggttt ggaaatcgta
     5461 gataccaaag taacgaagac ctatggtatt atccttttca aagatgcaga aggaacctac
     5521 gatattgaca gcttcatctt tcgagatgaa atgtacaaac caatctactt cttaaatgac
     5581 agtcctgctt actttaagaa aattccgaaa aaatggttga aattctttgt cggtgatcaa
     5641 gaaaaaatgg ttaagcaaca actagataga attaaagaag aaaacaataa accaaacctt
     5701 gatgacttat tagttgcagg taaaacttac tcaatctggg atggatcgta tgtcgctgtt
     5761 tatcagtata aactcaaacg tagctttatt tttagactac aagatggatc actcactcgg
     5821 ttcactcgac tatcagcagg agatgttaag gaagtagcct aaaacttaaa tacataaaca
     5881 taaacgtgta tacgtttatg tttatgttta tgtatatgta tatgaatata aacgtattag
     5941 gtacgaacgt ttttaaatgc ttttctgggc tttgatttta gggtagggct aaaacatcca
     6001 ttgatccaaa acagctcaga aacgcattta aacgtatctg ataccaatat tacagaagga
     6061 gtgtatcgta tgattaacct atttctttta gtgacagcta gtacattatt ctttactgga
     6121 atatcactga taatttccta tttgatctat aagaaagccg aaacttacac aagcaaagca
     6181 accggactag ccgtttcctc ctttttaact ttgctgactt tttttgttac tctatcgttt
     6241 tcttttgttt agcacagctg atccatcagc aaaaaacctc tctttacatt atacacacta
     6301 gtgtgtataa tgtaattgta aacaagctaa ttaactttta ggaaggagtg acaatatgct
     6361 aacagctaca ttacagcaac ctactcacgt tgctattgca gaaagctata caattaatgc
     6421 aggggtcgtt aaacagctga tcaaggatcg agaattcaaa aagtttaaca gtcgtcaagc
     6481 gtataacaga cagttaaaga aagctattct aaagcacaaa aattccttca tagggaaatt
     6541 ggaaagtgtg gatgttaaaa ctgtttcaac ggcattttca gttgttgaag tgactattga
     6601 cggtcgcttg atttctctat ttgcttttgg aattaattga tagggagtgt ggactttgga
     6661 aggtaagact tttgcagaaa tttggactga atttattgag gtaccaactc gcattgatcc
     6721 gataacttct gaaaaggtat tggatgcaga cttctacaat ctcaaagcag gtactagcct
     6781 tgaatgcgtt tagatgtatt tagaagagca tttcaaaggc gtaacgctcg gtcacgtctt
     6841 ataccacaat ggtgccgagt gggatgcaat tggaaatatc catttttctt aatacaagaa
     6901 aggagaacac aatatgaatt ataccgatga agaacaaatc actttggaat acgttttcac
     6961 aaatgaaaaa ggggaggaaa ttgaaaaagt cttgatcatt tcatcaaaag acttggagaa
     7021 cgctttgact tatgaaccat acgaatcaac cgtaactgtc aatgaattct tagataccta
     7081 tacttctaat gatacttggg aaattatgaa tatttgcaac gctaataaca ttcaatttgc
     7141 tgaaaaaacc aataaatcat tttattagtt ttgcaataaa acatcctctt tttctattga
     7201 ttccatacac aacaatgtgt ataatagatt ttgtaaaggg agggtagcta tgcctaagac
     7261 agcaagagaa attgtaaaaa ttctcaaatc taacggcttc aaaaagactg gacaaactgg
     7321 cagtcatgca aaattttata attccaagac taaaagaacg gtcgttgttc cgatccacaa
     7381 tggtgatgta ccactaggaa cagaagctaa tatctggaag caagcagggc tttaacagcc
     7441 ttgccttcct atttggtatt aggcatacta tcctctctta cactagaaaa ggagcgagga
     7501 aatgaagaca aaaaatattt tttatccggc aatcttcgag aaagaaggag aagcttataa
     7561 catcgttttc cctgacttac cggaaatatc tacctttgga aatagtcttg aagaagcata
     7621 tttaaacgct aaggatgctt taggattagc gctatatagt gtaccagatg cagaatttcc
     7681 taaaccatca gcagtggaag aattaacttt gaaagccaat caagtggtag taatcatcca
     7741 actaaacatt aaacttttcc gaagaagctt aaatacaaaa acaattagaa aaaatgttag
     7801 tgttccggaa tggttagttc ttttagggaa ggagaaaaac ataaacttct ctcaactact
     7861 tcaaaaagca ctagaagaag agttgtttga aaagtaaaaa ggagaccgaa cctatgaaag
     7921 ctacacactt aaactatttc tctcgaacgt taatcaataa tctattaaaa gatacaactg
     7981 gcattagcgt tagtaccaaa cactttgcca atgatctaat tatttttgag gataataact
     8041 tgcgaattga gatgtttatt gaacaggcaa cccctaactc aatacctgaa ttctgctata
     8101 cgcacactga aaaaatgaaa catgcaaaat cagttaaaga tgttgaagca ttaagtgaat
     8161 cactaacgaa cgctttaaac ctcgttaaga aggcaaaatt gttatttgaa tttcaaaccg
     8221 actaccacaa tgagttgctt gctcaatctg aaattttaga ttaattgaaa caggagaaga
     8281 gaaagatgaa acaaacacaa acagaaccca ttaacgatac gcttgctgat caattagcag
     8341 gatattataa attatttgag atgagtaaag cacagcgctt gatccactat ctttggaaat
     8401 acaaaatttt gtttcttttt tgggaagcaa cactaacatt ctcagttctt tttttataag
     8461 tcagattgtt ataatcaaca cttagtttta gggagttttt tgcttatcta caccattttg
     8521 atgcttaatt tatataccta catcacactt gaagaacact tagtaattag cgatgcaggg
     8581 atcgagagaa acatcaaaag ggtatctttc aagatcacgt taaacaagcc taccgataaa
     8641 aagctctcag aagcgttctg agagcttttg acattttctt gtataattta tccaaacaca
     8701 ttacaaagca cctaattcga gcttatatca agcaaatttg ctatttattc ctcaatacat
     8761 atctaaaaat tacttgatgg aaaactagat acatggtaag catgaaaaga aaatcaattt
     8821 gagcagatgc agtataatac atagcaattt aaaagctctc agaacgcttc tgagagcttt
     8881 taatatattc tttgataatt tgtctttctt aaatccatac gtcctaaaac aagctgtttt
     8941 tgcttaatat gatcatctga gagacatcaa atcaactttt ctctcaacaa atcattcaaa
     9001 gctttcgaca gattaatatt ctcttcagcc gctaaaacat ttaaatagtg ttccacagtc
     9061 actgattttc gatcgtaatt ttgcttgttt cgagctttag caactttcgt attagctttt
     9121 acgacaaata aattaacatt ctcagatcgc ttgattcttg catttaactc ttgaacaggc
     9181 gtaggcgatg gaatttcttt ttcttgcaac tctaaaagca ccaagtatcc ttccaatact
     9241 tcttgcgcca tctcaaaagc atcttctaaa gtttcaccat gcgtatataa gtctggaaaa
     9301 tctgggaatt taactgcata ttcatttcct ttccattcga atactgcaca aaaaaagtat
     9361 ttcatcgcat tgcacctctc taattaaaaa ataatttttg ctttttagtt tcaaaaaaag
     9421 aatcgcctac caaaaaaatt gataggcgat cacaagctaa ttaacctcta aaggagatta
     9481 ataccttgct cccaaggcac tacatatctt agcatacgtc tgaagcatct tcaaacgagt
     9541 aagatatatt ttgcattaac ctaatttttt ttctctataa ggtttttcat caaactcact
     9601 agaaatatta aactggacag caaaattgta atttctgcct tttctaactt tagcaaaatc
     9661 aatcttcagc attgattttt cattaatttc ctcaactgat tcccttaaaa cacgtctatc
     9721 aaaattcgat attgttttgt actcattttc tgttttcgta atctcacgaa ttttaactat
     9781 ttcaatcagg ggattcttat atatttcagc tttctcaaaa tctcctttat ttttggcgat
     9841 aagatatttt ttataataca tcgccaaata tttgtataac acaatgctat attttttttg
     9901 aaagcttgct aaatcttgaa aaacaaaata tggatatttc tttttgattg aaagaattag
     9961 gggaattact tcttgcgaga aattgaaagt tataatttgt tcttctaagt cccaatgaat
    10021 ttgatcaata gcagatactg tagcataagt cttatattct ttatctaagt tcgatatttc
    10081 ataaactgct tgtctctgaa gggtgttaac tatatttttt agttctgttt caagattttg
    10141 atcaacatcc atgtatttga ttattgcttt aacaggtaaa actatttccc ctgttttctt
    10201 taatttgtta ggagagatat aactgaacag caattgataa agttcatacg actttaaatc
    10261 cattttcatg tcaattgttg ctaagaactc ctcattgtac tttgtcagtt catctttttt
    10321 caaaagtttt tccttccttc acgcattttt tcagttgtta atcataaagg ttcacataag
    10381 gcaaataact cgtaattgat ttttcgagat atttaacagt attttttatt gtgtaatcct
    10441 ccttatgagt agcaacgtaa gataggtgat tgacaactga ttcaaatcct ctatctttgg
    10501 ataaacgatc ataaagcgga taaactttat tctgaagtcg gctcataagt ttaagatcgc
    10561 ataagtcggc agaactgatc aaaaagtttt tgattagtaa tttagtgtat ttgctattca
    10621 tagctttcaa gtacgattga ttcattttct ctgttttatc ttctgcgcct tttaaataag
    10681 cagggtctct ttgttcttgc ttataaggta ccggaactga catggtacgt gtaagcaaga
    10741 actttatgtt tgtataagta tgacccttct tgataagctc atagtcaatt ttaaaatcag
    10801 tgtatttatt cagttgggca atcggttctt taattacacg tctttcaaag tctcgaaaag
    10861 ctttatattt tttctcaata ctaaatttct ttcgtaaatc tgcaatggag aaaacaggat
    10921 tttttaatct atcaagtgat tttgtactcc atgttaagtt atctctattt gcttcataca
    10981 gattaaaatc tttagtgaat aattgaaaaa aactgatcgc atacttgctt gtcaagttaa
    11041 ctacttgatc aaatgtatat tgcgtaaaat gatcttctaa gttcgttaat aaaggcataa
    11101 ttgaactact aaatttaata gatactgcat cagaaaaatc attgaattca gtaatttcta
    11161 gcggtgagat gactttgtat tcagtcttac cgttaggttt agttctttga attttaatga
    11221 cagattgctg atgcaatttt tcaataactt gttttaatct tgaatgtttt gaagaagatg
    11281 aactagaaga catcaacgca aataaagcct ttttagatac tttgacggta ttattaaaag
    11341 gcggattttt agggtcaaga tgtcctatag cgtaaagata aattttttgt ggcatagcat
    11401 ccatttcaac aatgctatct acaagttgat tcgataaagt aacagttaaa tcaaactttt
    11461 ttagattgct ttcggtagtt tcaaatttcg ggtttacatt cttttctgtt attttcatac
    11521 gattgcctct tctttctaag cttggtacga gcttaaaaac ttggaactgg tacgattcca
    11581 ttgaataagt atagacctgt tttcgatacg ttacaataag aaatgtatcg taaatttata
    11641 acataactca atgtcagtaa aacacatttt attttttcca atcaaatttc attgaaaata
    11701 cagcagaaat cgctatttta gaacattttc aaaataaaaa tccttttcaa taaaatacga
    11761 aattataaaa attaccaaaa ttaaaaaaca aatatttttc tagtttttag aaataaattc
    11821 acatactttt tctttttttg gatatattcg gaataccaca tcgctttatg tccacgtttg
    11881 catcgctttt tgtccacatt cacatcgctt tttgtccatg ttcgcatcgc tttatgtcca
    11941 ccctaaaatc tatttaagat tgatataaag cctttttgga ggttgctaaa gttttaaaga
    12001 ttaaaatata tattataaat ataaataaaa ggggaagctg aagcttcctc cttttccttt
    12061 ttttcaatta ttcaaagtta cacaagcaaa ataaactcct agatagtgtt agtttttttt
    12121 aagatttttt atttttaaga ttttgaaata agagaggagt aatcctcctt tattaaatat
    12181 ctgttaatat atctgttaaa tatctgttaa tatatctgtt aggggaggct taatctcttg
    12241 ggagagtaag tattaaaggg gtataaaggt aacacttttg gacagtaaag gtaacaaaag
    12301 tggacagtaa gggtaacagt tttggacagc aaaggtaaca aaagtggaca tttgaggtat
    12361 cacactagga taagacgtta ccttcggttt tcttgatttg gaaggcgaaa aaatagaaaa
    12421 ttagattagt aacaaatctt tgttgactgt tacctttagt tgtgatttaa tttaggtaac
    12481 aagcagaaaa aattgtttcc tttaattagt accagattaa catgattaaa ggagcgcaaa
    12541 aagaagattg gagagtaatc aatgtctcaa gaagttaccg aaaagattat tgatagtgat
    12601 gtagatttag tatcagaaca aacaaatgca attgtgcgtt ataacaatgg tctgaataca
    12661 acagctttgc gaggatttaa gcctgttgaa atggatgtgt tttgggctat tttatcaaaa
    12721 atgaaacgtc aaggaacagc agaagtaact tttgatttca acctctttcg ttatttggtc
    12781 aaatacgaca gaagagaaaa ggacagtttt tattctgtgt taaaagattt gagtgacaaa
    12841 ctaggtaatt taacctaccg ttttgaagat tcagacgtct atgaacagct gtggcttttt
    12901 caaaaattcc ggatagataa acagaaggaa acagttacaa ttaaggcaaa tgaggaattt
    12961 gaatttatct taaactcaat tggcaaaaac tttactcgtt ttgaacttga taattttaca
    13021 aaactaagaa gtagctacac aaaagaattg tatcgtcatc tcatgcaata cagaaataaa
    13081 gagtggcgaa atggttattg ggtagtcaaa gttgaagatt ttagaagagc attaagtata
    13141 ccttcaagtt atagaatgac aaatattgat aaaagaatac tagagcaggc gaaagaagaa
    13201 tttttagctc ctgatgaaaa cggaatacag gtatttgaaa cttttgatta tgaaaaaatc
    13261 tatgcaaaga aaggaagaag agttgatcgg ctcagattca cttttagtga gccagaatca
    13321 aacttgccaa ccatttcaat gcataattgg ttagaagaag attgagaaga agcaggttgt
    13381 aattttaata caacctgctt ctttaaaaaa gtatgggtaa cacttttgga caataaatgc
    13441 aagaagtttt tttgtaagca agtttcagtc taaataatat ttgaaagaat ggctaatcta
    13501 tacaaatttt aacaactatg ggtaacactt ttggacaagt caagaaaaaa aacaattttt
    13561 cttaatttgt tgatcgacta aaggtaacac ttttggacaa cacagaataa aaagtccggt
    13621 gatatcagtt ttggtaggaa acatcttaat atctttgtga gattgaaggt aacatttttg
    13681 gacaagtcaa ctatttgtta aaagttaata tagagtaatt agataagttt tttcttttcg
    13741 tctgataaat ggataatatt agttatttta ttgctaatta gtctttcatg atgactgaaa
    13801 tagctaaaat aaatatctcg ttggataatt gctgatactt taagggatat ctaaatttgc
    13861 tgattgattt atttatgtta ttatgatatt ataataacat aaatgtgtta cgcccgaacc
    13921 tcattttaaa aaaatgaagg ggtgagacaa tgaatgctat taattggcat gaatttgtta
    13981 actatatgga gagcttacta gatgatctaa aaagttttag tattgctgat gcagtaaagc
    14041 atgggttacc agttagtaca acgatgaaat tacgaggatt gaaaattcca gatgaaatta
    14101 ttaagctgag aactaaaaca attattgaaa ccgcaaaagt cgttgacaat atgaaccatg
    14161 aactaaaagg caagaaaaca acttacgaga aagattttga acgtttttta aaatctgtta
    14221 ttggaaaata tctaggtaaa aaatctttca gtgaattgag agatgaagag attgtaccta
    14281 ctccaatggt tagcaaaatc cgaaaaaaca aagccgaggg gaattactat cttggtacaa
    14341 tagcaaaaat cattgattat ttggaaaata aagagcaaaa gaaaggaacg aagaaaggaa
    14401 gataattttt gtgttaaata ttaatgggga agaatatacc gaaatgattc aaaatcccga
    14461 acctagaatt accgaaaaaa cgtctgattg tttgctcttt tatgaaaggg ggcagacaaa
    14521 ctaatggaaa aaaacgtcaa ttcaacatct accaaagatc aatacagtat tattggaggg
    14581 ttgcttggtg ataaggataa gagaacacgt cacggattac gcattatgct tgcaactttt
    14641 ctaggagtgc tttttttatt aacaggcttg ggtagctaca tttatcaatt aggaaactat
    14701 aattattatc tcgtgaacaa tacaacacct ataggctctt cattggagtt tgctcgatca
    14761 ggagcagaga tcactatttc agatgtgtgg acagatcaaa acagagatgt aacagtagta
    14821 aaactcggtt actcgcaaaa cgctagaaag ttgttatcta cgcaaggaaa aaactacaac
    14881 ttatatatgg caacgaaaga aaaagatcgt cctaaaaagc ttgaaatgag ctatgggatg
    14941 ttaagtactg atggggatgc tttcttattc ctaaaaggta aattagatga aagagcttat
    15001 caagttttta ttgcaaacca agtatcacta atttctggtg atgatacagc tgacgatgat
    15061 accaaagtca atgtgaatga agcagattca gttacaaaag cactttctca atattctatg
    15121 aatgatgtta ataatgaagg agtaatttct tttgggaaaa aagcaaaacc tgttaatact
    15181 cctgataata tcaatttcag aataaatcca tattcagatt caactaatgt ctatcaagga
    15241 agctttctta ccaaagatgg cgatattgat tattcaaaag ttgtagcagt tacaagtaag
    15301 aataatgttg taagtaagtt agaagatcag ttaaatgctt caaatactaa catcaagaaa
    15361 attgaagcta caaaagctga gtatctggaa cgtttacaac aaaatcctga cgataaagat
    15421 tcgcaagaaa gtattcgtca actagacagt cgaatggaga ccgaaattaa agcaatcaat
    15481 gatacacaaa aattgattga tcaatacaaa aatgctgact ttaacaaaga ttcttttgga
    15541 gacatgaaga cgacatttaa gtacctaact ctgactgact agaagtaaat tattcttatt
    15601 ttagaggaag tgaacacaaa cagatggaaa atagtaccga gttttttaag aatattcaaa
    15661 ctgaatttaa tcatgctctc acaccaatga aaccaccgaa acataagtta gtgggacgtg
    15721 atgacgaact ccatgagttg tcactgatct tgaatagacc ggaaacaccg caagctatct
    15781 tgttaggtga agccggaaca ggtaaaacag cacttgcaag tgaatgggct gaaagacagc
    15841 gacaagatgg aaagaaagta caagtataca aattatcaat cggacaattg cgtgttgggg
    15901 gagatgatgt cctccaaaca cgtttagaaa cattaattcc aagattgaaa gaatatgagc
    15961 gaattttaaa atctgaggac aaagaaaatg aatttgtcct cttcattgac gagtttcata
    16021 aagttgttag ctcttttggt agtggcaata aaattggggg tgattcgcta aaagaaccgt
    16081 tagcaaatga atcacttcaa ttgattaccg ctactacaag aaaagaatat gaaaactata
    16141 tcaaaagaga tgatgcctta gggagacggt tacaaccgtt agaattgaat gaactaaaaa
    16201 gcgatgttgt cgtcgatatt ttgagacaat ggctaaatac atcctctgtt aaagatgatc
    16261 ctcaggcttc ttttaaatta tcgcaaagga ttcctgatga attacttcaa gaaatcgtac
    16321 aagcaaacaa gctttatcgt gaggatttgt ccgaaccggc taaatcaatt gccgtaatgg
    16381 aggatttgat tgcaaaagat cgagtagacg gaaatgctta taatcgagaa agtctattgc
    16441 aaatatttgg taagtataat attcagctag attctgattt tgatatggaa aaacttcaag
    16501 aggaattaaa gtccataaaa gggcaaccgt tagcagttta tactataaca atgttgttta
    16561 aacgtatgaa gcttggattt gatcgtttta aaggtcgaaa gcaaccaaaa gcaactgctc
    16621 tgtttacagg atcaacaggg gtcggtaaaa ctgcaatggc taaagccgtg gcaagaggac
    16681 tttatggcga tgaaaataaa gttctcttag ttaatatgcc ggactacaaa acaaaagatg
    16741 atgcaatgcg ttttagacga ctcataggta ctcatgtaag gcatgatcct tctgcgatta
    16801 ttgtgtgtga tgaatatgag aaagctggtg tagaaacact cgatgccttt ttatcaattt
    16861 tagatgaagg taggataagt tttactgaaa gaggatggga tgatcgagaa gttcacgaca
    16921 ctgtttcatt gtcaaataca attttatttg cgacaacaaa tgcaggtgcg gagatgttcg
    16981 attcgttaaa cagatatagc tcactatctc gtgttacaaa caaggaaaat tctaatgaat
    17041 atagcactga actaaaaaga gaataccgaa aagtcgcatc agggatcaaa caagccttga
    17101 ttggtgaagg tctaaagcct gaagttatta accgatttgg acgaatagtt ccattttggg
    17161 ctttatccga acaaactctt ctggaaattg cttataatga aatgttgaag agtgctgaat
    17221 tagtacgaaa acatttaaat atagatttac gtcttccacc taggaaagaa agagtatatg
    17281 gtcaaaattt ttatgcagac gatctctcca tgcacttagt ttttgacatg gcttcacaag
    17341 aggattctaa atcagggggt gcaaggaata tcttgcattt agttgaatct gagtttgaag
    17401 aagcgattac tgaagcaata attacttatc caaacgcaaa aatattcgat gttaagttga
    17461 gtgaagccgg agcgtttgaa ggaaaaggtg attctaatgc tgaaattatt gcaaagcctt
    17521 ccgttgctga aagtaaagaa aacaagattg tattttcgat tggaggggcg tagctatgaa
    17581 attcaagaaa tatttactac aaaaccttag aataaaacga tgggtcgtaa gaggtctagt
    17641 tattggagga gtattagcag ttactttagg cgctcctttt ggaatgaatg atccaactcc
    17701 tgacaatcaa gatagcaaac aagaaacagt tataaataag aacgttgcat atgcggaaag
    17761 taaaccggca gaacaaggaa tgtggacact atatgctcgt atcctaatgg gagataaaga
    17821 agttaatgat agtgccgcta aaactggaca aatgaatttg ttaggacaag gtggtatcag
    17881 tgccgatttc ccttattctg acatttctaa acaaggtaat tcgattgcag ggggtaatgc
    17941 aggtactcaa ctagcatcta ctttagcaac ttatagttac tacggatata ttgaaacagt
    18001 ctcaggaaat gctattgcca gtcaagcatc atccattcta ggaagtattg gtaggacgat
    18061 tggtagtttt atcggatcaa ttagtttaat cctttctaat attgctgaat ccataaatga
    18121 actccttgga aaatttttca tgagtatgaa tatattttct cttatggggt ggggagaagg
    18181 tggatcggat gtatctaatc cggtatctaa agcattaact agcttgctta acaatttggg
    18241 attaaataat gaattattta aagcgatagc aagcttatct atgctctttt tcacagcaat
    18301 actaggttat caaggaatga aattattgtc gaaagatggt gttaactcga agggattatc
    18361 accagtactt gaaaaatgga ttgttcgtat ttttgtattg tttatattct taccagtggc
    18421 aggatcgcta ttgggagttt atatcaacgg catccagtca gcaaaaaaag atggagatat
    18481 accaacaaat gttgtttctg actacatcat taacacaaga ctttgggctt ctactcaaaa
    18541 tcttgctcca tcatctgata ctggtggtga tgtacctgac ggaaagccta catccgaaat
    18601 caagttcgtt gatacagatt atgaaccatc taaaaaatct gcaatgataa gagcgatcaa
    18661 tactagcagt tatgccagac aaaataatga tctagattct aattcagtcg gtatggctct
    18721 attaaaagaa tggtcatcga atgatacttt caatattaat acttatgccg gtgatgttaa
    18781 tgtcgccgaa aatgatgctt atatgggaag agcgcctgct gtttggacag gctataattt
    18841 aggggatgga aaagaaggaa gtgcaattaa gaaaccaacc aatttaaatg gatatatttg
    18901 gtcagcaaat caaaatgtga cagacgacaa tcgcaagcct gatggaaaag gaacagcgca
    18961 taactttgat gctactcaac cttggggcgt tgctaacaat gcaactttta gcacacagtc
    19021 ggtagcttta ttgctccaat cggcacttga tacatcggga gctaagtttt atgcgtataa
    19081 tatcgctcct tcaggaattc aaggcagtgc taagaatatg tcaacagtaa aaacagaatg
    19141 gcgtacagca tccttagttg gtcatggagc tatgggtaag tttggctcgt ggctagcgat
    19201 gaacgctgaa tcaatcgttt tagcagttct ttacttagca tgtgcttggg cgatgttcaa
    19261 tgtgaatatc gtgaaagcat ttaaacgtga atttattcgg atttgggaag tagttctaac
    19321 aggatcactt gcatcaggag caggtgtgat ggcaatttca ttaggtgttg ttacaacatc
    19381 tctgatcgga ctacaattac ctatctttct gattactttt attcgtgcat ttggtgatac
    19441 tgctgtggga ttcattccta atacattagt tggcgatggc gtagcaaata ttgttcgatc
    19501 tgctatgatt ttaggtgtag gatatctttt agtctttaaa aaaggcgaaa aagtaaataa
    19561 tttgattaat atgcttattg gcttaccact tgaagtagcg tttgggtttg atgaacgtgc
    19621 taagttatta tttggtaata gaaatgatgc tgtgcgagtt gtggacggcg tttctaaagc
    19681 aatcaagaaa agccaaggag ctaaaaatcc ctattatgat ggtggcaaca gctcagtaaa
    19741 agatatggtt gcaggaggtc ttggagcagt tggagcgcaa aaaatggctg aacgtttgaa
    19801 caatatggga gatagcaatg ctgatgctaa gtccattgct gataaagtaa ttcctgaaac
    19861 accagaagga atgtcattca acggtggcgg agttggttcc acaatcaata atacctcttt
    19921 caaagattta gttcgtcaag atcaactacg taataactcc caacaatata ttgatcattt
    19981 aggacgtatg gttgatgaaa acggaaactt tgtcgatgaa aatggtgaaa tattaaaaga
    20041 tagtaaatac ggtaaagatg ctaaaggcaa ctttattgac gaaaaagggc gtttggtcga
    20101 tgaagatgga aaccttatag atactaatgg taaaccattg gaagatagtg aagatggtaa
    20161 tattggtatg gatagcgaag gacgtttaat tgatgaaaaa ggtcgcttag tcgatgaaga
    20221 tggaaatctt gttgacgaaa atggacgcag gttgctaaat ggtgattttg gtcatgatag
    20281 taacggacgt ttagttgacg aaaaaggtcg tttagttgat gaagatggaa atcttgttga
    20341 tgagtttggt aataaacttg gtaaacacag cgatggatat aatgtgaatg gagatttagt
    20401 cgatgaggat gggaatatca ttgagacgag agatgcgctt gtggcagatc gaaatagaga
    20461 actcgaacaa caacggcata cgccaattgc tgttgcaatt cctgagaatg aagaagacga
    20521 gattacttta gatcaaactc atcaagaact gaggaatgaa atgagtggtg aggacgtgtc
    20581 agacagagtg catcgggaat taggagatgg agcatctggt gaaagtcagt caagagagct
    20641 taacagcaac tttgataata gtgctattcg gaatgacaca gtacttaaag acagagaatc
    20701 gctaacaaaa gaaacggata aagaacaatc agatgtgaag aaaaagttca ttcctactcc
    20761 taatgttaat caaacagata aatatgaaac aaatattgga tcaggaatgt cgaaagacaa
    20821 tcaatcaatt agagaacctg aactgacaaa taaaaataat agaacaaata attctaaatc
    20881 tacttcagat aattcaggga aatctaatca atcaaccaag aaaacatcaa cagctaaatc
    20941 agccgttgaa tatctatcaa aaactggtaa aaatgtttac acctcagcag ataaagcttt
    21001 agcaaaagct ccaaaaggta gtctagcagg gataacctat gaagctaaaa acgaagctat
    21061 taaaataggt aaaggaacgg ctaaggttgc caaggctact gctacaggag catccaaaac
    21121 tggtaaagta gtgaagaaat ctaccatgtc agtcaaggac tatattgtaa aatacggtaa
    21181 tcaaaaatca actgaaaata agtctgaaga cgagtaaaaa gaaggagcat tgatttgctc
    21241 cttcttttta ctttgatttc taactgaaga tgtcaaggaa aatttctatt tatgcaaaaa
    21301 aattatttta aattgtctat aaatgaatta aatatttact gttcaaagtt gtataattaa
    21361 aaagggctta tgctggtaaa gaacagaatt attatctaaa caaaaacttg ataaaccagc
    21421 atttttagcc aataaaaaaa gtgaaataat ttaatggaaa atctctttta atcatcgaaa
    21481 atttatgtta ttatgataac ataaagatgt aaatatcgag acgtcttttt cttagttaga
    21541 tcattgacaa gtgaacaaat aagatttgtc gatgatataa ttaacttatt aaaaatatta
    21601 aaaaggaggt ttttatatgt tcgattctat taaaaatttg gttagcggaa tgacgcctga
    21661 tatgaaaact ttagtacaag ttgttttgta tttcttggca ttcgtttttt cgttgaaacc
    21721 aggattcact gcaatgaaat tttttggtga tgcaaaatgg ggagatggat taaaatatgt
    21781 tggggcgatt attgcaatct ttattattcc tgtaattaca accgttatgc tttacacaat
    21841 gggtactaaa acaggtaatg atttgaacca aaaagctaat atcatatcag cacttattcc
    21901 agttgttggt atctatctag tttcaaaatt tccaaaaaat gaactgttag gttaaaagtg
    21961 aattgaacaa atttggataa tatgccgatt tgttcaattt actttttacc aaaaaactac
    22021 gctccaagcg tagtttagaa gtgagggaag aaagtaaatg taagatggtt tttgatagag
    22081 aaaaaatgtt agctcacgcc aacgaagtat taatgagcag tctgaaaggt accgaactgg
    22141 caaagataat gaatatgaat gttaaccagt tctatgatta tcgaaatggg agtaaaaaga
    22201 ttgaaaaagc cagacttgaa acgcttatta aattcgaaaa agcatatgta tatatgctag
    22261 ataaacagaa aagaactatt gactgaaaaa aggagtttta caataatggc aaaattcaaa
    22321 gatattaccg aatctattaa ttatgtggca gaaacagttc ctgtagttgc actctctttg
    22381 aagccacaat tactcgaagt gtacaaaaaa gaagaaaaag aagcgaaggc aaaaaaagct
    22441 aaagcaaagg tatggaaaga attcgatgcg ttgctgacca aagatttacc aaagaaaaat
    22501 tatatggtaa ttgaagatgt acaaaagctc aacagtgagg gagttataga tgaggatgat
    22561 gctattctcg attctgagaa gtatgtcgtt ctattgaagt caagtttgaa ttatgtagct
    22621 gatctaccag aaaaatttaa agagaagcta ttagcaggtg tccgttttat ggatagtagt
    22681 ggtcaattat tagttattcc ggatgatcct aatgtgtttg aatcagaagt tgaagaagct
    22741 acagacttct atagcgcagt agagcaatta gaagtagcta ctaatggatt agaacgaatt
    22801 gctgagacct tcaactcttc tgaagaagtt gaatttgaag ccgtagcaga aaatgtacca
    22861 gatgaaaaag aagctctttt tgtggatgat aacaaaccaa ccgaagcaga aaaccaagaa
    22921 gtaagcatag aagaaacaca aatacaaaca gaaacctacc aacaaccaaa aattgatgaa
    22981 aaacccgaat cccaaagtga caaagaaaat tatgtttatg aagctgaaaa aatcacatct
    23041 gaaagtgatg taccgaaaaa cttagatgct gattttttag aaaaaaattt tgaacagatc
    23101 attgctgaca aatttcaacc tgtagtttta gatgagatta atgaccctgt cgatttaaac
    23161 gaggagatgc tatccatacc tgagttgaaa ccattgctgt ttttagctaa agagagtatg
    23221 aacgctcgca tctctattgc taataataaa atcaacagtg aattaaataa gacgaaggag
    23281 caattgatcc tcaaaggaaa gcaaatgtta gctgatgagc ttttgcggat agaggaagca
    23341 acctcattaa atggtcaaaa tcagtttaca aatacactat cggttgctaa agagagctac
    23401 gaagcagaag catctaaaat tgatgattta gtgatggaaa aatccaatat cattcgtaaa
    23461 gattatgata tggcgaaaca ggcttatttg gatgctgaac taaagaaact agagcaacgg
    23521 tatgatgctg agcatttacc agaagttaaa caacaagaag atgaatatag aaaagcaact
    23581 gaagaaagtc ttgatcaaca gtaccaagaa gcaattgaat ctattaaaaa cgatgcattg
    23641 aaacaaagcc aagaagcaaa agaagttgtg gtggaacgaa ttatcaattc attgaaacca
    23701 gaaattgaaa aatcattaac agacgtgcaa gaaaaaactt tagagattgt cgaaaaaaca
    23761 aagtcagaaa atgaagctga gttccatgat acaaaagaga aaacaacaga tgtggttgtc
    23821 aaatattttg cgaacaaaga agaagttgaa aagcaggtag ctaacaacac gaaagaatta
    23881 gaaacaaaac ttgtagagca acaaaaaact atcgaagaat ataaagatcg aatgaaagag
    23941 gtcaaagatt tagcagaggg ttataaatta aatagcgaaa agttggcaga acaaagtgaa
    24001 gctttaagag aagacttatc tcgttcccga agtgatctag ctcaaatggc agaaaaaacg
    24061 aaatcattag cagttgctac agaaaatcaa tcaagttcaa aacgtgattt tacaagtaaa
    24121 aagatggcta ctatcatttc tgtcgctgca atggcttgcg gaacgatctt gggatcgtcc
    24181 tactttatta gtcataataa tcaacaagca caggctcaaa ctcaaacatc tatttcgaga
    24241 gtggaaaatt cgattgggac aaatacgaac tctgcttcaa caaatagtag ctatgaagca
    24301 acaccaacta cagagttcaa agttgggaaa gatgttgtca tcagtattga tgggaaaaca
    24361 actaaggctg aagtgatttc tattgatgga gaaaaagcag ttgtgaaaac tccagacgga
    24421 aatcagtaca aaattcaagc tcacttatct gataataact aaggagggct attatgcgag
    24481 aagaaaaaat agtttatcca gtaattcaaa acaatcaaga aaacatgacc tcttctcagc
    24541 acttacagga agcacagctc gtgtatcagg taaatgaact accacaaacg aacgagacaa
    24601 tgagcaagtc aatatcttta attggcttgc ttattgttgc aatagtgata actgtaggtg
    24661 tttaccgtaa gttgaacgtg aaaggaagta tttctgatgg cagataactc aaactatgga
    24721 tttaaacata acttacatca aaaaccttct gaaacaattg tcagagttga atggcttgaa
    24781 aagcatgtca atagcaccta ttctgttaca gcaaaaagat taattgtatc agctatagtt
    24841 gttgcagtcg ttgttcttgg atcaattgtt tacttagttt ctgatacgat taaactgaaa
    24901 tttatttttg ttccaacgtt attaatgata attggatttc ttattctaat tagccagtat
    24961 atgtttattg atccatctag tggttcattg atttctggtt ctattagact gttcaaatca
    25021 gtagttaatt cagcaagagt atctatgggg aaaaagaaac catttcgagg cacagagatt
    25081 ttaaaagtga aacaagatgg aacaatcgta tatcgagatg ggagtgtagc attaatgttc
    25141 aatctagatg gggctacatc actaaccgct tttccagaag aagttatggc tcaggaatcg
    25201 aaatcaatac aatatcaaaa taccagagat cgttccttag ctgaaatcaa aatcacttca
    25261 tctcaacctc agaatacgga aagacaaact cgaaatatga aaatacaaaa acgaaatgca
    25321 aggagtaaca aagcagtctt tgacataata gatcagcaag aacgatttgt atcagaatat
    25381 attgatggtg tgaaaacaac agtaatccaa tatcttatct tgaaatctca atctaagatg
    25441 gtattggatg aaggtgtgga acgtttagaa aactatacga gtagaggact ttattatagt
    25501 gtcactcagt tgtcagctaa acaaacaaaa gaaatattat ctgatattta tgcactgaaa
    25561 tagaggtgag agattaaatg gaaattatgg gtagatcaca actattaaat ttcaaaaaaa
    25621 agagaaaaac taaaaaagaa caaaaaacta gaaattcaaa ggtagtgaat aatacctttg
    25681 ttagtgattt aacatcttta gttggttatc aatttacacc atcttacatt aggacaggta
    25741 agcggtgggg aacaattgtg aaattagtga atacttatgg aatgaataga aattatggat
    25801 ttggttggat ggttaaagct attcctgaag tgagagtaga aggggtaaaa gcctatctaa
    25861 ttgaagcaga taagccagtg tcacaaaaag atcaacaaga aataatgcac gagaatgttg
    25921 ggggatcaac gaatagttat gaaaataatt tagaaggaaa aaattcagat gctgaaatta
    25981 gcaaaactgt tatgcggatt atggatttga atacagcatc tttactagac agcatggcaa
    26041 ataaaattat tgattggaag ctagaaatcc tattagtatc cgattctcct aataaaatca
    26101 gcgaacagct aattaaactc caacggttgt atgatgaaaa aatgtatgga ttaaagctta
    26161 tgtcatatgg aggagatcaa cgtgatctgt ttgatagact tttaagtgaa ccaatcggga
    26221 atgtctatga gtatacgtct atgagttcgg attttgcagg aaatgatcat gcaattcgca
    26281 aaggtttaga tgatgcgaat ggatgggcta tcggcactat gtctactagc tacgcaagtg
    26341 gtgtagcttt tatggcactg gatgattctt ttacaagtaa agttttagtg gcatcacaca
    26401 aaaacgctac aattaaaggc tatgatgaaa aaatctcagc atcatcactt tgggggcaat
    26461 tgattgctaa taatgcaatg gcaaatcata aacgaacttt tcatattgtc ttaaataaat
    26521 ttgattactt tggtgatcca actggtagaa catttgttgc caaaccttcg ttaaacagtg
    26581 taatagaaaa ggttaatttg gctgaagaag gtcttaactc tttggagctt tttggtaatc
    26641 aaaaggacat tggtgattta tatagacgga atctcgataa actaactcag attttttatt
    26701 tgttagcagg cagaacatta gattctgcaa ctagagtttt attgaaaaaa gaattaggta
    26761 acttttatat aagtcagaaa ctttgggata ttaattatga agaacgttca aaacttttgg
    26821 tgaacagtag tgatacaatg ccagttcttg gggattttat tagctatttg actaatgctg
    26881 aaacaaaagc caaaaaatct ggtactgaaa aagaagtcga tcgtattatt gtattaaaag
    26941 acacattgag aacagcatta gacagcttct ctaggatgtt taatcaaaga acgacactga
    27001 aagaaatcgg taaaagtgac aaattacagt tttactataa cttaggtgat ctaagagcag
    27061 aaaccaatgt tatggaagct caatttttaa atacctttga tttcatcaca tcacaagcaa
    27121 aaaaagatga tattgttatg attcacggct tcgataagtt atctattgaa accgttgggc
    27181 taattaaaaa aagattaaca atgcttgaag aagatggaat tcgtcttgcc tttctatttg
    27241 atacaatcgg ttcaggagaa gacaatgaat acaaagaaga tattgaaaca gctgatatat
    27301 tcaatacaga tggaaaaatt taccaaagtt ttgaaagtga ttttaattac actatcattg
    27361 gaaagatgac cagtggagag ttaatgaagt atgaaaaaaa agttgctcct gctagaaaag
    27421 gagaagatga tcaaaaactt tctgttgatt tgagacagta tttgacaaca ggaagaagta
    27481 ataaatttca gatcaggaga ccagtggatc tgactaataa ttttgttatt ggagagttta
    27541 ttctttaagt tttatctttg attttgagtg tataaggaaa ggataagaaa aaacatgtat
    27601 atttgagact aaaaacagtc ttagagaaca tggtacgttt tgtgaaaaga gaactataga
    27661 aatttcataa agtgaaacct tattgagtga aataattata gtggaggtta aataatgaaa
    27721 ccaaacataa aaattttaga tcgaatattt ctaggtagag acacagaagt aatcctaatt
    27781 caacatgaag aaggatttga agttagtata ggtattcaga aacttcaaaa acctcattat
    27841 tgtaatcagt tgtataaaaa ctttactgat gaagaaaaag cgagagtttt ttttaatgaa
    27901 gtcaaaggta tgagagaaca gtatgaggtg gttgaagcat gaataagtta ccagaaaaga
    27961 accgtgtatt tagattgaaa ggtgatctca gtc
//
LOCUS       DACKAC010000035        27913 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.035, whole genome shotgun sequence.
ACCESSION   DACKAC010000035 DACKAC010000000
VERSION     DACKAC010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27913
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.035"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(18..710)
                     /locus_tag="I0B46_001549"
     CDS             complement(18..710)
                     /locus_tag="I0B46_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAR2083857.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            705..971
                     /locus_tag="I0B46_001550"
     CDS             705..971
                     /locus_tag="I0B46_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAR2083858.1"
                     /translation="MHRTFGSRGCPTLHLADRYAERKTPTLCFAFSARTRQAGIPLED
                     IKDFLGHKDVSTTQVYAHISPEVKKRSMNQLENYIEEQIKKHSN"
     gene            complement(1147..1716)
                     /locus_tag="I0B46_001551"
     CDS             complement(1147..1716)
                     /locus_tag="I0B46_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAR2083859.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYKTLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1790..3079)
                     /locus_tag="I0B46_001552"
     CDS             complement(1790..3079)
                     /locus_tag="I0B46_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083860.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFYFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLIKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQALAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            3433..4281
                     /locus_tag="I0B46_001553"
     CDS             3433..4281
                     /locus_tag="I0B46_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAR2083861.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4426..5367
                     /locus_tag="I0B46_001554"
     CDS             4426..5367
                     /locus_tag="I0B46_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAR2083862.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTAYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5357..7528
                     /locus_tag="I0B46_001555"
                     /pseudo
     CDS             5357..7528
                     /locus_tag="I0B46_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7640..9811)
                     /gene="copB"
                     /locus_tag="I0B46_001556"
     CDS             complement(7640..9811)
                     /gene="copB"
                     /locus_tag="I0B46_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAR2083863.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(9831..12017)
                     /locus_tag="I0B46_001557"
     CDS             complement(9831..12017)
                     /locus_tag="I0B46_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAR2083864.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(12017..12226)
                     /gene="copZ"
                     /locus_tag="I0B46_001558"
     CDS             complement(12017..12226)
                     /gene="copZ"
                     /locus_tag="I0B46_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAR2083865.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12239..12679)
                     /locus_tag="I0B46_001559"
     CDS             complement(12239..12679)
                     /locus_tag="I0B46_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAR2083866.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12753..13292)
                     /locus_tag="I0B46_001560"
     CDS             complement(12753..13292)
                     /locus_tag="I0B46_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HAR2083867.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
     gene            complement(13907..15373)
                     /locus_tag="I0B46_001561"
     CDS             complement(13907..15373)
                     /locus_tag="I0B46_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAR2083868.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(15684..17765)
                     /gene="cadA"
                     /locus_tag="I0B46_001562"
     CDS             complement(15684..17765)
                     /gene="cadA"
                     /locus_tag="I0B46_001562"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAR2083869.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            complement(18289..18648)
                     /locus_tag="I0B46_001563"
     CDS             complement(18289..18648)
                     /locus_tag="I0B46_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAR2083870.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(18678..19019)
                     /locus_tag="I0B46_001564"
     CDS             complement(18678..19019)
                     /locus_tag="I0B46_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083871.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            complement(19016..19690)
                     /locus_tag="I0B46_001565"
     CDS             complement(19016..19690)
                     /locus_tag="I0B46_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAR2083872.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(20743..22041)
                     /gene="asnS"
                     /locus_tag="I0B46_001566"
     CDS             complement(20743..22041)
                     /gene="asnS"
                     /locus_tag="I0B46_001566"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAR2083873.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(22081..23271)
                     /locus_tag="I0B46_001567"
     CDS             complement(22081..23271)
                     /locus_tag="I0B46_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAR2083874.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYSLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(23292..23819)
                     /locus_tag="I0B46_001568"
     CDS             complement(23292..23819)
                     /locus_tag="I0B46_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAR2083875.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(23866..26655)
                     /locus_tag="I0B46_001569"
     CDS             complement(23866..26655)
                     /locus_tag="I0B46_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAR2083876.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(26802..27002)
                     /locus_tag="I0B46_001570"
     CDS             complement(26802..27002)
                     /locus_tag="I0B46_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAR2083877.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(27454..27774)
                     /locus_tag="I0B46_001571"
     CDS             complement(27454..27774)
                     /locus_tag="I0B46_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083878.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
BASE COUNT         7845 a         5918 c         4904 g         9246 t
ORIGIN      
        1 aaagaggggg ggatttttta tcgttgtgga atatttaaat accaacgctt gtcatgaaaa
       61 tcttgtgctc cacccttagt atttccttct ggatcgtttg tcgcacgcat catgacgtat
      121 actttcttat tagggaaatc acgcatattg aaagatacat gatagccaac atttcctaaa
      181 gtattataag cttgatttac gtctggtcta taaatcccat cagctcttac tctagctagt
      241 tcttttccag tattgtagtc cataatgaaa atatactcgt atttataatt agcaatgtgc
      301 catccagcta catgcaagtt tgcgttttcg atttctccga actgatcaat gtgggcgtaa
      361 tttgttccat ctgtcagcgt agaatttgca gcacctgctc gagttggatc aattactggt
      421 ttatcctccg aagtagttgg attatcatcc gtaaaaccat gagccaaatc ataggctagt
      481 ttttctttac ttacacccat ttcagaaaga taaccgtaag gatctgtatg atcgccccaa
      541 atattttgtg ttacccataa atgcgatttg attcctggtt ggttataagg agtgtctaat
      601 gttaatggaa taccatattt cattgctgaa tctcttgcca attcaacgta tactttatag
      661 ttcttttcaa aagtttcttt atcatgtgtg tgttgtaact cgatttgcac aggacttttg
      721 gctcacgagg ttgtcctact ctacatcttg ctgatagata tgctgaaagg aaaacaccaa
      781 cattatgttt tgctttttca gctagaacga gacaagcagg aataccttta gaagatatca
      841 aagatttttt aggtcataaa gatgtttcca ctacacaagt ctatgcccat atttctcccg
      901 aagtgaaaaa aagatcaatg aatcaactag aaaactatat agaagagcaa ataaaaaagc
      961 actcaaattg agtgcttttt taagctcact atcactaaaa ctatcactaa ttttatttat
     1021 aagcgaacat gtttctatca ctgatttttt aaatctagcg aaaaaccctt atcttaaagg
     1081 cgttcgttca attctttggc aaggtcttca aagcctggct tgcctagaag tgcgaacatg
     1141 gttttcttat agctcatgta gaaaaatttt cttgatgatg attttatcca tcaattgaga
     1201 ttgactgttt tgctctccta tgatttcagc tatgattgtc cgattttgcg gttttcttct
     1261 taactggtag ttgattttcc cagtttccat caactcgcta agcatatcca cgactccgcc
     1321 aaatccggtc tttttcgtaa taaaacctag tgctaccatt cgttcatcta gattttttgc
     1381 tgtaatcgtc acaccagtaa tgagcgagtg ctgtaatgtt tgggccagct cttccatccc
     1441 ttcagcattg gatacgacgg aagctgtttt gttaataggt tcttcgttta tttcttttgg
     1501 ttctttcctc tccactttca ctgtttgacg aaggacatct agttcttgca agagcgtttt
     1561 gtattgttct tgggtcagca tcacgccagc tactttttca cgattaaata tatatacgcc
     1621 agttcccgcg tctcgcgctt gagcaaatac atccatcggc gatcgtttca cttctgtgat
     1681 cgaagatgtg ggaacttcta atttctttaa ttccaatgga tttcctcctt tactaatcgc
     1741 tttttattat agcacagact gacttgtttt gatctttttt ccaaaggttt tatcggtttt
     1801 tgcctaacca gtaattgata tcggtaagaa gtctttcgat accaaatgaa ggatggtaca
     1861 gatacgtttt ttgatcttcg ttatggtcta gtggaaaaaa atgatcggtg atcactagat
     1921 ctgcataccg aggcggctta tttcgagtac atacatgtat agagatccgt tttcgatgtc
     1981 ttagcgtttt gttcagtacc ccagcaagtc ttcttgcttc ttggtgttca tagcgaaaaa
     2041 aaaagacgat tttcacttca ggagggtgga catctaaata ccctcgttcc tggagtgttt
     2101 tcagtaaaag catcccccac tctactgcgg ggtgtgcaag tgcttgttgg atcgctagcc
     2161 gaaattgata tagtttatct ttggaattgg gaaatttttc taatgtttcc tgataataac
     2221 gaacttcttc tatcagccgg tcatggatac caattacgta acgaagccat tcttggtaaa
     2281 gtacttttat cagctttgat ttgctttcgc tctcaaattc gctattcagc caaaacagcg
     2341 aatcaagctt ttcagtcaca atcgtcaatt tccgatagtc ttgtgtctct tctatccgtt
     2401 tccaccgctt ttcagtaatc tggttacagc tcaagcgcaa aattgctaga taattttttt
     2461 cttttattgg caaaatctgc tcctctctaa atagcaattc aggaaacaat tcaaagaata
     2521 gtgttcgact gtctcctaaa atctcgattt ctgccggatc ctttctttgc ttcaataatt
     2581 ggatgaacat ctcagaaaag gactgctctc catcatctgc aaaaaaatgc ggaaaatctt
     2641 tcaaaaagta aaacagcaga tatcttactt ttctttctga accaaagatc tgtgcttttt
     2701 tataagaaaa agaaaggtgg tactcgccaa accactttct caatcgctct tgcgtagaac
     2761 gatcgtactt ctgctcgtaa ggttcattta atagcagatt tttcagtaaa caaaacgggg
     2821 gtgattggat cagaaaaaca aacaagagat aaggttcgct atatccttga tcataaacta
     2881 actgaacggt tttcttatga agaatcagct gaatgggaca gtcgctttcc gccaaacgtt
     2941 tcagatgccg atccactgtt ttaacatcga ttttcagttc ttcagctaaa tcagcttttc
     3001 gccagatcaa atctttcatt agattgatta gtgaaaataa attctgtgca ttttttgata
     3061 atagtttttg ccatctcatc gatcatttct cacctgattt ctaaacatat tgtttgatca
     3121 aaatgagttc actcctaaaa agataaaaaa ctccctttct atttatttaa atacgcaaat
     3181 cttcctcatt attttttatt ttactcaatt taatcgtatt ctcatttcaa aacgaatgta
     3241 ctttaaaaaa acataataag atcactttgc ttgtaggatg tattcccttc cattacaatg
     3301 aaataaatag cgattttatg agaaggtgat gtgaaaatgg aagaaaaaat agaagaatcc
     3361 aaacaaaaaa tcgatcagtt gatataagaa ttagaaaaag cggtcatcgg caaacgttca
     3421 actatccaat tgatgatcgt ggccctatta gcaggtggtc atgtcttatt cgaagatatt
     3481 cccggtgtag gtaaaacgtt actggtcaaa gcactagcaa aagctgtaca gggaaccttc
     3541 agcagagtcc aatgtactcc tgatctgctg cctagtgata ttcttggttt ttccgtatac
     3601 aattcacaat caaaagagtt tgaatttcgt ccgggaccga tctttaccac gatcttgttg
     3661 gcagatgaga tcaatcgtac gactcctcgt actcagtcag cacttttaga agcaatggcc
     3721 gagaatcatg caacgatcga caacaataca taccctctag acaatcattt ttttgtctta
     3781 gctacgcaaa atccaatcga gtacgaagga acatatcctt tgccggaagc acagctcgat
     3841 cgttttttat tccgactaca aatcggttat ccttcttttg atgacgaact tttgctactt
     3901 ttggataaat tcgaaaaagt gttggataac ttaaatgaag tattgaacag ttatgaaata
     3961 gaagaaataa aaacaaatgt ggcgaatgtc tttgtcagtc ccgaagtggc atcttatgcc
     4021 ttgcagttag tgtcagcaac aagaagtcat gctgccatcc agcttggcat cagtccacga
     4081 ggaagccttt cctttattca ggcagcaaaa gcttatgctt taatccatgg gagaaattac
     4141 gtcacgccca aagatttgca ggatttagtt ccttacgtgt tttcccatcg cctgattttt
     4201 tacgatcgtt cattgaagga agacgaaaag aaacaaatca ttcatactct tgttgcacaa
     4261 attcctattc ctgtgaggta gattgaggta gacaatggaa aaaatcaaag attatttcat
     4321 taattttgtg ttatttctgg tatatttcat cattgtgctt tttgctcttg tattcaacaa
     4381 ttctttaggc tggttcttgt tggccttttt tactctttat gtatcatgga gcttgatttc
     4441 attaagcagc tttctaaaga aactttcggt tacttgtaca ttgcagccac tcgtttatcg
     4501 caaaaaaagt atgcaggtta ttttttccat agcaaaaaag agaacttttt ctttacctct
     4561 ccctcgttta caaatcggct tcccagcaat ctttgcagaa aaaaacaaga gattttgatt
     4621 cttacaaata aaccgcaaga agtccaattg ctttggcagc caaaagaaag aggatgccat
     4681 gaaacattaa ctgtcatttt tacagcctat gatagtctag gacttttcag aaaaagtgca
     4741 aaaagagaga tctcttttcc tgtgactgtt ctgcctgctt tttgtaagat tcaagcagaa
     4801 aaattacaga gagttctgga aaaaaaacag cagctgaacg cgtaccaaag aggtcttgat
     4861 tttcgggaac atcgcgctta tcgtccaggt gattctttca accgaatcga ttggaaattg
     4921 tctgctcata ctcaagagtg gttataccgt gaatacgatt atcaagaaga agaatgttct
     4981 cctctttttt gcttttgggg ttcgcccagt ccaaaatttg aacctctgct agacttgtat
     5041 ttttctttat ggcatttgag aaaagaaaag cagccggaac ttctgatatt aggcaaccgc
     5101 gcctttcatg gcaaagaccc cggtcatacc tattttgctt ctttggaagc tggcgatgag
     5161 aaagcttttt tcgcacactt gaaaaaatat gcaaaaaaac aaatcgtcct ctttgttcct
     5221 gagaattctc ctgaagtctc agaagctatt gaaacattgt caaaagatcg aacggcttat
     5281 cttgtttatt tcgtagaaaa acaactcatg gtccaagaaa aagacaagat atgttcattg
     5341 ccaggaggtg aatggattga cgattaaacg ggaaaaatgg ctgcgtggcg tttactcctt
     5401 tgtgatacta agtgtaggtg ctccgatatt tgtagtagct aatcaattgc aaaacagcta
     5461 ccttcttatc ctaactattg gtgtcctatg ccttattgtc tgctgtattc ctaaaaaatg
     5521 ggtacgtttg ctgctttctt tgccagtaat gataggttgt ttgtttttat attttccttc
     5581 gaagacattt tccatattat ggatcttaca gtttttttcg tcagttacag aggaattctt
     5641 ccatctatcc caaggtaaac tttattattt gccagaaaag acagcttttt ttctcataat
     5701 gttgctgatc tatttattta ttacattgac cgtggattac gactattggt acgcaccctt
     5761 tctttctttg atggcttact tcatgatgct taccgttttt cggaaaaaag atttactgta
     5821 cgaaatgatt gcggtagtaa ctgttttgtt cgtgtatctg gcagcaaggc aatttttctc
     5881 catccggttg ttgtcgggaa acactcgatt ccagtcattg actacagttc tgcttatttg
     5941 tttcttaact tttagcgcta tcttgccttt atatcttccg aaagtacacc aatctctcga
     6001 ggaaacgagt gaaccgatcc gagaatatct taatgatgaa gggctttatg acacactcca
     6061 tgagtatcaa ttaaccggaa gtgcaagaac aggcttcagt gagaatgatg aacagcttgg
     6121 tggaccttta tatgataatg gcgaagttgt ttttacagcg aaccaaaaag gaaatggcta
     6181 ttggaagatc gaaaataaaa atcattacac cggttctgga tggatagaag atcaggacgc
     6241 acccatccaa ccaatccaag aattccctgt tgacatcacg agctaccaaa ccgttataga
     6301 agacaaagaa aacattacat tgaccctaaa aaaacaagct ttcttacctt atccttatgg
     6361 aaatactacc ttgactgatt tttcattagc tagtggacaa acgctttact atttgcctga
     6421 aagccagcga ttcattcctg tcccgaatga atcgacacgt tctgcaactt tacaaacaga
     6481 aaggttagtt tttacgagag aagaattaaa aagcagtcag ttaaaagctg atgatttaga
     6541 aaatcagcag acaaatcttc aattgccaaa aagtttgcct aaacgtattg aggacttggc
     6601 ggtcgaattg accaaaaatg aagcaacttt aattcgtaaa gtcgaagctg tctaacagta
     6661 tttaaaaaca agcggaaatt tccgctattc caaaacggat accgggcata ctccgaaaga
     6721 acacgattat gtggattatt tcctctttga ttcaaaagtc ggctattgtg acaacttttc
     6781 aacttctatg gtcgtccttc ttcgaacact tggtatccca gctcgctgga caaaaggttt
     6841 tacaaatggg ctcagaacag gcacagataa tcaagggaat gaggaataca gcattctcaa
     6901 tagtcatgct cattcttggc cagaggtata ctttcctgga tttggctggc ttccatttga
     6961 accaacccct tcatttgcca atccaatggg ttcacaacca gttgctgaaa caaccgaaac
     7021 cgtccagcct gctatcgaac aaccaaatga aagcgaaaat atggcagaaa ctatttccag
     7081 tgagagatct gaggtcattc catcagaaga aacaacactc acaccagctt ataaaatcgt
     7141 cttattcagc cttggagtgt gtttatccgt cttgttgttc tttctattgt tattcagaaa
     7201 atctctgcgc tggacaagtt atcttatcta tcttcatttt tttgcttcta ctaatacgat
     7261 tgcatatcaa aaacttctta accctttgga aacattggta ccgcgcaaac ccgcagaatc
     7321 tttgcaggaa tatagcctgc gaatagaaca agaattcaag ctagatctgc gtttcattcg
     7381 attaactact caatatgaag cagaaatata tggacgacaa gcagatccgg taaaattcga
     7441 taaaaaaacc tatttagaaa taacgcgtat aataagaaaa acggtataca aaaaaattct
     7501 gacaaaaggg agccgcttca aaaaataaga taaaatttct ggagaatcta cttctggaat
     7561 gccgagaacc agctgattga gtatcgatta ctcgaaaaaa aggttgtgac acgttttttg
     7621 tcacaacctt ttttcattat tatttgattt tcaatgtcaa agcatttaga gctacaacta
     7681 ccgtacttaa cgacatcaag acagcaccaa ctgctggact taatatgata ccaattggcg
     7741 caagaattcc ggctgctagc ggaattgcaa gaatattgta acctgccccc caccagaggt
     7801 tctggatcat tttctttcgc gtttgcttgg ctagatcaag aaaatgcaaa atatcttttg
     7861 gatcgctgtt tgtcaatacg acatccgcag aatcgattgc gatatctgtc cctgcaccga
     7921 ttgccatccc gattgaagca cgggctaaac ttggcgcatc attgatccca tcacccacca
     7981 tgattacttt tttcccttgg tcaaggtatt tctggacaac agcttcttta tcatctggta
     8041 ataaaccgcc ataatattct tcgatcccta aataatctgc tactgcttgg gcagcgtttt
     8101 tattgtcacc agtaagcatg acaggagtga tttttctttc tttcaatgtt cggataaatt
     8161 cttttgcttc tgtttttact acgtcaccta aagcaacgat tgcagctaga tgattttcta
     8221 aaatcaggaa gctgatggta tttccttgtt cttggtaagg tttcaataat gcttgttcta
     8281 cgtttaatcc gagacgctca acttcttttt cattaactat tttcacttgt tgttttttga
     8341 ctgttgcctc tagcccgact cctgaaagat tttttagatt ttcagcttga taaggcttga
     8401 tttcatgttc tttcaaataa ttcataatgc caacagccaa tggatgattg gcattcgctt
     8461 ccaaggctcc tagatattga aggatttcat ttttattgaa cgcatcatca agtacttcga
     8521 cacccgttac agtaaatttt ccttctgtta gtgttcctgt tttgtccagc atgatcacat
     8581 ctagatcatt ggcttgctcc agtgcattgc ggtttttcaa aagtaatccg tttttagctg
     8641 ctattgatgt ggagcgagca acaactaatg ggatagctaa acctagagca tgaggacagg
     8701 cgatgataaa gactgttacc atacgttcta atgcattagg aagatctgtc aagaacaacc
     8761 aggcgatgaa tgccaggata cccacaatta atgcgacata aaacagccat tttgccactt
     8821 tgtcagataa tgactctagc ttggattttt ctccttgtgc ttttcgcacc atttccataa
     8881 ccttcgctag ataaccattt tcaccagttc cagttacttc gatttcgatc gttccatccc
     8941 cattgataga accgccaatc actttgtcat tggcttgttt tttgattcct cttgattcgc
     9001 cagtcacagc tgattcgtca acaattgttt cacctttcaa gattacacca tctgttggca
     9061 ttttatcacc agaccggacg atcaaacgat caccttcgtt tacttctttc aatgaaactg
     9121 tctcttcttt tccatctttc gtcaagcgtt tcactgattc tggcagtaat tctgctagtt
     9181 tttgtaaagc attgctggca ttcgacacag cattcatttc aatccagtga cctagtagca
     9241 taataacgat caaagtagcc aattcccaga agaaatccat gacgtgtgta tgtggattga
     9301 gcagatttgc gataaacgaa tagacactat aaatatatgc aactgtaatc cccatcgcga
     9361 tcagtgtcat catagcagga cttttttgtt tcaattccat cttagctcca cttaaaaatg
     9421 gctgaccgcc atagataaat agtatagttg ctaagatcaa tacgagccaa tcagaaccag
     9481 gaaacgttac ttgaaatgga aattccattc ccatcattgg tgaaaaaaga atgattggga
     9541 tagctaggat aagcgatagc caaaattttt gtttgaaatt ccccatgtgc atggaatgat
     9601 ccatgccgcc gtgttcgtga tccatgtgca tcgaatggtc catttgtttg ctgtttgcta
     9661 tatctccatg gttatgtccc atgtgcatgg aatgatccat ctgactatgg tcatgtttca
     9721 tctccatctg tttttcatga gattctgtac tttctttgtt tactacttct tttttatgct
     9781 catgtttttt ctccatatcg ttcccattca tttttactcc atcccttctc ttactttttc
     9841 atgtgtcgat ttaagctcag ggagttcaac aatacactga cggaactaaa cgccatcgct
     9901 cctcctgcaa tgattggatt caaaaagcct agtgcggcaa aaggaatacc aatcgtatta
     9961 tagacaaaag cccagaaaag attttgtttg atttttctca atgtcaacct agacaactcg
    10021 attgtttgtg caatggatgc caaactgcta ttcatcagcg tcacatcggc tgtttccatc
    10081 gcaatgtccg ttccacttcc cattgccata ccgacatcag ctaatgctaa ggccggtgca
    10141 tcattgattc catcgcctgc cataccgact tttttgccat ctttctgaag tttttctacg
    10201 taggctgctt tatcctctgg aagtacttca gcaaagatat gctttggatc aataccaact
    10261 tgtttaccaa tcgtttcggc tgccagtttg ttgtctcctg tcagcataaa aacatctaat
    10321 cctttattct ggagctgctt gattgcttgt ttggcttcta gtttgatttg atcagccacg
    10381 gcaatccacc cgatcacttg cttttcatca gctaaaaaca tgactgtttt tccctgctgt
    10441 tctaattcta acgcgtgttc ttggtattca tcaaatgata ggttcaattc aattaatcgt
    10501 ttacgtgtac ccgcaaaata gcgtactcca ttaatcgtac cgctgatccc agcacctggg
    10561 tgggctgtaa aatcaatcat atcgtatgtt gctgcttctt gcagttttcc gtattccacg
    10621 attgctttac ctagtggatg ttcagaagct tgttctaaag tataaaaaat agataggact
    10681 tcttttgaac cgactagatc tgtaactttg ggctttcctt ctgtaatcgt tccagtttta
    10741 tccaaaacaa tgctgtctaa gtgagcagct gcttctaatg cttctccgcc tttaattaaa
    10801 attccatttc gtgcaccaac acccgttcct accataatag ctgttggggt cgctaaacct
    10861 aatgcacatg gacacgcaat gactaataca gagacactat ggatcaatgc gagttgccaa
    10921 tcaccagtga tcaaacctgt agctatgagc gtgataaacg cgatgactaa tacgatcgga
    10981 acaaaaatcc ctgagatccg atctgcgatt ttttggattg gggctttgct cccttgagca
    11041 tcttccacca tttgaatgat ttgcgctaat actgtctgct tgccgacttg tgtgacttcc
    11101 atgtgtagca gaccattcgt attgattgtc ccgccaaaaa gcgtgtcatc cggattcttt
    11161 tctacaggaa gattttcacc agtaagcata ctttcatcta tcgcactgct tcctgaaata
    11221 attcgaccat ctgctggaat ctgctcacca ggacggatga ctagttgatc accaacgact
    11281 acttcttcaa tcgctaacgt ttcttctttc ccgtttctta tcacttgggc ggtttttgtt
    11341 tgaagagaca tcaattgttt gattgcatta cctgttttcg tttttgctgt atgttccaaa
    11401 tatttgccga gcaaaatcag cgtgatgatc atactgctgc tttcaaaata aagatcttgg
    11461 gggtgtccac ggaaaaatcc gttgtaaatg ctcaatgcaa aggcagccga tgttccaatt
    11521 gctaccaata catccatatt tggtgctttc gtcttgattg catggtacgc acctttataa
    11581 aaacgagcac cgacataaaa ttggacagga gcagacagca ctaactggac gattggaaaa
    11641 tgaaaaaagt gaacaatcgc cgcatggctt cctaacatca tagcaatcat tgacaacatc
    11701 aacggcaaag tcaaaaccgt actcaatata agatcgaata gcattttttt caaatacgct
    11761 tgcttttctt ctgcaatttt ttgtttgtgg gcttcatcgt acaaaattgc accataaccg
    11821 atgttttcta cgctttggat taatttttca gtggtcgtcc cttcatattt gacacttgct
    11881 ttttcagtgg ccaagttgac tgtggcattg accacaccag gttgtccgtt cagctctttt
    11941 tcgatcctag cagaacaatt tgcacaagtc atacctgtaa tgacgaacgt atccattttg
    12001 gtttctgctg ttgccatcaa atcacctctg ctttataacc caatccattg attgcttgac
    12061 aaatcttttc ggattgaact tttgtttcat caaacttgac cgtaccgttt gcttttttca
    12121 aatttacttt cactttttgc acgccgtcaa gtgcgcttac tgtttcttct actcttgctg
    12181 cacaatgatt gcagctcata ccttcgattg aaaattgttg tttcattttt tcttcctcct
    12241 attgatgttg tttacattca cattgacctg ggatacagtt acattcgatc gtatctactg
    12301 gttgcttttg ttccagttgt ttcaaaatct cattgatatc ttcttgagtc aaagtggctt
    12361 cttctactag atccgcaatc gtctctccaa tcctttttgc acagatatgg gaaaaaagat
    12421 tttccgtcgc actgcgaact gtttccatct ccgagacagc tggatgataa atgaattttt
    12481 ttccttcttg ttctgtccag atcacttctt tttttaccaa acgtcccagc aaagttttga
    12541 tcgttgctgg tttccaatcc atcgaatcag ctaagatctg cgtgatttct tgggctgtcg
    12601 tctgtccaag tgtccaaata acacgcatga tctcccactc tgaatcactg atctttactg
    12661 gcgttttgac tttttccatt tatttcacac ctttcgttta caaatgtaat cattagctta
    12721 tgaatacaga ttacgcttgt aaacaaaaac tgtcaattat ttttgctctc tattttcaat
    12781 ttagttaaaa attcttgttt ctctcgtctt gttttaagga tgatcagttt atttcttgca
    12841 ttagctactt ggatcagctg ctgtattttc ggttcattgt tttcttcaaa atgccagata
    12901 aaagataaaa attctaagta ttccctgttt agtttctctg aaaatgattc aggcatatct
    12961 ggccgagatt ttttgtttat ctttcttttt agagaacgcc aaactactcg gtagatggcg
    13021 agataacgcg gcactttaag ccaaatgatc ttatctgctt ctttaatcct ttcctctaat
    13081 gtagaagtat aatttccttc aacaatccat gacggattag ataccataaa cgattgctgt
    13141 tcttgcaaaa accacttttt tgcctgaata ctgtagtctg ttgtatgcca taaaaggtct
    13201 agcggcagta gcggaaagtc ggtgatttct ttgatcttcc ttgcgagggt cgattttccc
    13261 gacccacttt gtccaatcac gatgatcttc attcttctcc ctcacttctc tttggaattt
    13321 ggttcgatca taccacttat aacgccgcaa aaaaacaagg gactgtgaca aaagtcgtgt
    13381 caaagtccct tgtttcgaat aaatggtgtt caatcagctg accttcagca ttaactcaaa
    13441 aaacggcaaa atatgaggag ctattttttc gtttgttcct ctaatactca ggtcaaatcg
    13501 ctgattttac aacctcttcc ttaaacggtg ttctcctgcc aacacctcgg tcacaaggca
    13561 caatagtcaa caaacatgag aagttgtttt ttgaaattgc tccttgtgac tcggagttaa
    13621 acggcaggct cactacctct ttcttaaacg gtgttcaaaa gcttgcatct gcggtaataa
    13681 gcccagcaaa acgaaaaata tgaggggcta tttttgtttg gctgttctta ttacttgatg
    13741 caggacagct ttttcacaac ctctgattca cggtgttcca gaagtagctt ctgcgaaaaa
    13801 caagccgaaa cttcacaaaa atttgagaag caattttcgt aaattccttc ttatttcttg
    13861 aagctgacca cttctgtcac aacctcttgt aacaagtatt tctattttaa ggctgaacca
    13921 ctgtttctgg ttcagtgatt tctttgtttt tgtctcgacg agcaattgct ggcaagatca
    13981 tacctaataa gatcaagaca attggtgtga tgatattcgt gaataattgg aaccaccatg
    14041 ctttaggatc agcagtataa tctacttttg gcaccattcc taggatacat gcaaacgcag
    14101 tgaaggcaaa gcaccaagca ccaaagataa aaccgatttt tggattttta acaaatttat
    14161 attcagaatt gaatttctta taagctttat tcaacatcat atatgccaag aacacccata
    14221 ggtaacgcat tggcatgaca actgagttca agttcgtcaa ccattttact aattcattca
    14281 tatctttgat accaaagatc ggcaacaaaa taatgattcc tactagtata cctgtcagaa
    14341 gataaccatt caccaatgta cctttacgat tacgtttacg tagccagttt ggtacaaatt
    14401 ctggatccgc atctgctaac aagattttta gtggtgcatc aattgaaaag gctaatgcgg
    14461 agatttgtcc aatcatgttt gtcaatgcgt aaatgatcat caatgagtta ccgataccat
    14521 agtaattacc taaaatttga aaagcactgt atgccccatt tgccatcaag tctttaggaa
    14581 tattatcgct agcaaacagc atacccattg cgactgagcc taatacagca caaatcccta
    14641 ccattgctgc taagaagatc atccctttag gaaattcctt tgcaggattt cttgtagagt
    14701 tgacataagg ggatattttt tccgctcctc caactgcaaa tactagcatc gaaacggttg
    14761 tgaaataact gaaatcaaat gttggaatat atgttttcac ttgattcata tgtggtgtgg
    14821 caaattccat cgttggattg atgaacggtg ccgttacagc taaaagaata aataagatcg
    14881 acatgacgaa catcgctgtt ccggccagtc cgccaatcac tttcaaagta gaaagtcctt
    14941 ttgttgataa atataaaaag gcaagaaata taataagtga aataactgct accatttgta
    15001 ctgacatcgt atttacgata tttccatttc cttggactgc ccaaccgcca gcaatcagga
    15061 ttgcttgagg cttttgtgcc aaataaggaa tatggacgac ccagtaagtc caagcggcat
    15121 agtatgccag tcgtttatta gatgtcgtat tttcgaccca tgaactgacg cctcctttac
    15181 tatccttgaa ggctgaccct aattgtccaa cgatcaatgc gtatggaata aaataaatgg
    15241 ccaaaatcaa tagccaagag gtgacaactg atactccttg ctgagcatag ttattaacta
    15301 cattccccat gccccaaacc atgttaaatg caattaacgc aacagtaaac cagcgtaatt
    15361 gtttgttgtt cattcgttct ctcctttatg ataaaaattt gttatccaga tgtaatcata
    15421 ttcaattttt aacaaactta caagacagtg ttattttatg tgtgtattct tttatttaat
    15481 acacatcgtt ttttcatatt ttttccatca aaaaaataag ttattttctt ttatttcatt
    15541 taccttattg aatttcaagc tattttgaat tttctgcaaa attaaaaaca ttagttattt
    15601 tataatccta tctattttct ttacattttt cattaagaaa aggatataaa aaaaagagga
    15661 cgttcattcg tcctcttcat tgatcaacgt ttatttttta catgcaagct tcgcatggca
    15721 ttcaaaacag ctaatactgt caccccaaca tctgcaaata ctgcatctcc cattgatgca
    15781 aaaccaaaag cacctaaggc aagtaccaaa atttttatcc caatagcaaa gacaatgttc
    15841 tgtttgacga ttttcaatgt tttctttgct aaatggattg cttctgcaat cttcgatggt
    15901 tgatcattca tgatgactac atctgctgct tcaattgcag catcgcttcc caatccaccc
    15961 attgcgattc ctacatctgc tcttgctagc accggtgcat cgttgattcc atctccgaca
    16021 aatgcggtct ttttattgtt tctttgtaag atttcctcta accgctgcac tttatcttct
    16081 ggaagcaatt cgctatatat cttgtccacg cctacttgct tcccgatatg atcagcaatt
    16141 tttttcgaat cgcccgtcaa catgactgtg ttcttcacac cagccttttt taattgaacc
    16201 aatgcgtcta cggcatcttc tttcaaggta tctgcaataa caagataacc actgtaactt
    16261 tgatcgatcg ctagataaag caatgtaccg atttcttgac ttgaaggaaa agagatttca
    16321 aatctttcca tcaatttgtg attacctgct aatacttgtt tgccatcaac ttcagctaag
    16381 atcccttcac ctgcaaattc ttggatatta gctgttggta aaagcggacc tgcgtagcct
    16441 tctaaaacag attgagcaat cggatgtgta gaaaactgtt ctgcactcgc aacatattgc
    16501 atgaatatct ttggatcgat ttttgtatcg atcgtctgga tcgaaaagtc acctttcgtt
    16561 aatgtgccgg ttttatcgaa taccactgtc tcagtttgtg caagcaactc caagtaattt
    16621 gaacctttta ctaatacacc gattttactt gctcctccga taccgccaaa gaaactaagg
    16681 ggtacggaga tcactaatgc acatggacat gagatcacta aaaatgtcaa tgctcgatag
    16741 atccattcag aaaacgccgc tccagaaaca acaagtggag gtaaaacagc taataaaacg
    16801 gccaatccta caacgactgg tgtgtaatag cgcgcgaatt tagtaatgaa attttcagct
    16861 ggtgcttttt tactgctggc attttctact aattctaata atttgctgat agtagaattt
    16921 tcataagatt tttctacttg aactaaaaat ggtttatttt tattgataaa tccacttaat
    16981 actgtttcac caacatatac agatttaggg actgattctc ctgtaagtgc tgacgtatcg
    17041 actaatgatt ctccttcgat gatcgttcca tctaacggta cacgttcacc aggcttcaca
    17101 agtacaaact gaccgatctg cacgtcttca gggttcacgg tcatcaatcc ttccgtcgtc
    17161 tgcacgtgag ctgtttctgg acggatcgcc atcaattcgc gaatcgattt tcttgaatga
    17221 ctgactgcca atccttggaa atactcgccg atttgataaa atagcatcac tgctacggct
    17281 tctggatatt caccgatcaa catagcgcca atcgtagcaa cagacataag aaaattctca
    17341 tcaaagattt ttcctttgac gatattcatc actgccttct tcactacatc atatcctatc
    17401 agcagataaa tcaataaata ccctatcaag cgaattggtt cattaaaatc aaataatagc
    17461 aataaagcga tacctgcaag ggaaaataaa atccggaaca gcgtatttct ctctttttca
    17521 tttccatgtt cagatacttt ttccttatct atttctgtta ctttgacatc tggttctaat
    17581 tccttcacta ctttttttat ttcttgttca atcgtagcca aatgatcttc ctctgcgtca
    17641 atcgttagtt ttccagaagt gaagtttacc gttgcttcct tgactccatt gatcttctga
    17701 actccttttt cgattttcat tgcacagttc gcacaatcta aaccttctaa tctgtaactt
    17761 ttcattttaa tgacctcctc gcctcaacat atgaatatct cttcataact aattataaat
    17821 gaacggttat tcatatgtca aggtatccga aagaaaaaat gaaaaacatc tgcatcggaa
    17881 aatttcttcc atacagatgt tttggcttat tctttctttt tcttttcgat cattgccact
    17941 gctcttatcc aacccgcact tgccttcttg atcttgacag gatagcaact aagtttgaag
    18001 cctgttgctg gcaatttgtc taaattcatt aatttttcga taggacaata tcttttttca
    18061 atccctgcaa aatgtccttc ccagatattc gaagctccac tacgctttca aaaatataga
    18121 aagagttctt taatgaaaat tatttcaact ttccataaac agtaacccca tataaataaa
    18181 agacgtaaaa gtcacatgga agaacttata caggagaacg catagagtca ggtgaaatac
    18241 atgtatccca cctgactcta tgctttatct aacctttatt attctctttt atacaatttc
    18301 tgcaccgaat ggcatcaacg ccaatcgagc aagtttgaaa gattgcttgg caaaaggtat
    18361 ccctatgatc gtgatacata gaaggatgcc gctgacgaca tgtgcgagag caattggtag
    18421 gcctgaaacg atcagccaga tgatattaac aatcaatgac atgccagact ggtgataaat
    18481 tacttctttg ccaaaaggcg caaaagaaag ggaggcgatc ttaaaacatt gcataccgat
    18541 tgggatacct atgatcgtga tacaccaaag tatccccgct gctaaccagc taagaccacc
    18601 gattagtccg ccgaatataa accagatgac atttcctaaa caactcattg tatttctcct
    18661 tctattactg tttttgatta aaactgggca aaaaatgatt ttattcttct ttttttagct
    18721 tccagtatca aatattctgc aaattttttt tggatttctt ccgtattttc ttttgttact
    18781 aatgtgtgtt ggattagttt tcctgatggt cgctgatcca atcgttcttc tgactgttgc
    18841 acgtccgctt gcgcaaatcc gataatccgt ttttcttttg ttacagcaac gagcaatagt
    18901 ttattcttat catttaggtc agctgccgtt tttgctttta cgaatgattc tggatgatca
    18961 gtaatcagct gattgatttg aggtagatct tcttttactg cccaccgtat ttccatcatt
    19021 tctcactttt ctttcgctca tttttatctc cactaaaaaa atcgactgcg gcaattgcat
    19081 ctatccagct ttgtgcaatg atctcatgtc cgccaatcgt tgggtggacc ccatcttctc
    19141 ttgtatattt cgaaaaacca taaacaatcc ctaaagcatt gaatatacca tctaaaggaa
    19201 ccagcaatgc gccatattcc ttagcaagtt tacgaacaat ttggatttta gggtctaagt
    19261 ctttacgcca gccatgacgg tcgtcggtta taggaagtac aaacggctcc atcaagatga
    19321 tgttgcagat tcccactgtt tgaagttcat ttagtaattt tcgataaacg gattcaaatt
    19381 gctgagcaga tttctcagtt ccgaatatag cctgatcgct agtattatgc caggtatcat
    19441 tgattccgat caaaatcgat acatagtcag gttgtaaagc taaacaatct tcttgccagc
    19501 gtacttgcag atctgcaatt ttgtctcctc ctatcccacg gttcatgcat tcaaattcca
    19561 tcgttggata ttgggagatt agttttccgg caatcaaaag tggataacct ttacctagat
    19621 cagttgcttc tgctcggtct cttcctgcgt ctgtaatgct atcaccgata aataatatac
    19681 gttttttcat ttgtcaaaaa ctcctttttg aataaaacga tgattttctt ccttatatta
    19741 tcccataagc cctatcaaga cgaaagagaa aatatatgta taaaaaatga caccagccta
    19801 gtcccttcaa acaattgaat gaacgctcat caagtgtcta gttttttatg ctattttttt
    19861 cttaacgtgc cggattcccc ttgattcctt cgttgtttgg tccagagaga tcgatcacac
    19921 ctaagcttcc gtcaatcaga ccactgatga cgccatcaga acatatattt ataggctcat
    19981 ctaagacttc tagtaaatgt ccctttacta atatctcttc tagattgctt gggatagatt
    20041 ggtcataact ttatctggtc cgatgatttc aagggtgaat tgaacttaca tatggattat
    20101 tgcatgctga aaatacggac ctactccatc ttcgaatgtt actattagtt tgttttcagg
    20161 tgctagatgc tgctttattt caacagcaga agcatcatca aatcagattt ccatttgaat
    20221 taccttcttt gtaaatgttt gattgattgc gatttcgtta tagaaccttg tgaatcaaat
    20281 agcaatttct tttgctttct tctcttgtct gctggaaact tcctttatcc cgtattttaa
    20341 aaaaagaact gcgagagttc gcagttcttt tttagaataa acggtgctct ccggccaact
    20401 cctcggtcac aagtcacaat agtcatcaaa catgaaaaac tgttttttga aattactcct
    20461 tgtgactcgg agctgaacgg caggcgcaca acctcttcct taaacggtgt tcaaaagcca
    20521 gtttctcggt attaagccaa atttatgcaa aaatgtggaa agccatttcc acaaaatttt
    20581 tcttaatact cgaaactaaa cagctttttc acaacctctc cactaaacgg tattcaaaag
    20641 cttacatctg cggtaataag tcccgccaat tcaaaaacat gaacagctgt ttttgcttgg
    20701 ctgttctaat tacttgatgc agaacagctt tttcatcacc tcttatggat aaattcggtt
    20761 caacaaacgt gggaatggaa tagcttcacg tacatggtcg ataccagcaa tccaagtgac
    20821 tgttcgttca agacctagac caaaaccaga gtgcggaaca gaaccgtatt tacgtagatc
    20881 aagataccat gaatattctt tttcatccaa accgtgatta cggatttgtt ctagcaagaa
    20941 atcatagtct gttgcccgtt cgcttccgcc aataatttca ccgtaacctt ctggtgcgat
    21001 catatctgca cagatcacca cgtcttctct tgttggatgt ggtttcatgt agaatggctt
    21061 gatggctttt ggatagttta ggataaagac aggctgttcg aatgaattcg caatgaacgt
    21121 ttcatgaggt gaaccaaagt cttcgcccca ttcgatgtca tcaaatccat ttttcttcag
    21181 taattcgatc gcatcatcat aagtaatacg agggaatggc aattttgtat aagtttttaa
    21241 gacttctttg tctcgtccta gtacatctaa tgcatattcg caatgatcta aaacattttg
    21301 tactaagaat gctacatatt gttcctgtac ttctaaactt tcttcttgat gagtgaaagc
    21361 catttctggt tcgatcatcc agaattcgat caaatgacgt cgtgtttttg atttttcagc
    21421 acggaaagtc ggaccgaatg aaaagacttt tccgaaagcc atagcagcag cttccatgta
    21481 taattggcca ctttgagata aataagcttt ttgatcaaag taatttgttt cgaatagatc
    21541 cgtagtgcct tctggtgcag aaccagtcaa gatcggcgga tcaattttga caaaatgatg
    21601 ttgattaaag aattcatacg ttgcacgaat gatttcattt ctgatctgca taatcgcatg
    21661 ttgtcttgaa gaacgcagcc ataaatgacg atgatccatc aagaagtctg ttccgtgttc
    21721 ttttggtgtg atcggataat cgtggctctc gcttaccact tccacatttg taattccgat
    21781 ttcataacca aattttgaac gactgtcttc acggatttct ccagttacta acacggatgt
    21841 ttcttggtgt agatttttag ctgtttggaa aacttcttcc gacacttcgc tttttacaac
    21901 tacaccttgg aaaaaggctg ttccgtctct taattgtaaa aatgcgattt ttccgcttga
    21961 tcgcttattt gcgacccaag cgccgattgt tactgtttct cccacatggt ttttcgaatc
    22021 gatgatttga atttgttcca cgtatgactc tccttttata ttccttgaat ttctttagga
    22081 ttaattttga cttttactct caataaattg cttgattctg cgtaccgctt cttctaacgt
    22141 agcgcgatca gtagcataac tcatgcggac attttctggt gccccaaagc cttcgccagt
    22201 cacaagcgct acccctgttt cttctaaaag atcttctacc cattgtgtga cattttcata
    22261 tttacacatg cgtagcgttt cttttacatt aggaaaaaga taaaaagctc cttgtggctt
    22321 ttcaagagaa acacccggca gttctgccac aagcgaatat aacgtattca ggcgttcttc
    22381 aaaggcgttc cgcatttctt ccactgtatc ttgctcgcct ttcaatgctt caacagctgc
    22441 atattgactg actgctgtcg gattactggt tgactgggaa gcaacagcga tcattccatt
    22501 gatgatcaca ggatttccca ccgcataacc aatgcgccag cctgtcatcg catatgtttt
    22561 agacacgccg ttgatgatga tagtctgatt tttgattgct tctgaaatcg ttgcaattgg
    22621 agtaaactca ttgccattat aaactaaacg accataaata tcatcagcaa ctatcaaaat
    22681 atcatttttt actgcccatt caccgattgc ctgcagttca ttcttgctat aaatcattcc
    22741 agtcggattc gatggagaat tgatgattac ggcttttgtt ttttctgaac gtgcttgttc
    22801 tagctgttca acagtaactt tccagctatt ggattcttcc cctttcacaa agacaggaag
    22861 tccttctgcc agttttactt gctcaccgta actgacccag taaggcacag gaatgatgac
    22921 ttcatcttgc gggtcaagga tcgtttggaa aagggtatac aaagcaaatt tcgctccgtc
    22981 tgttacgact gtttgtgaag gttcatagac aagtccatag tttttcttca gataatcaac
    23041 gattgcctgt cgaagctctg ggattcctgc tgttggtgta taatagctag ctttcccgtt
    23101 gcggattgct tcgattgctg cttcttggat gttttctggt gtcgcaaaat ccggttctcc
    23161 cacagtcaaa ctcagcacat ctctgccttt tgcttttaat gccttggcct ttgccgcagc
    23221 tgccaaagtg acagacggtt ctaatcgttt tgttcttttt gataactcca tcccattcat
    23281 tcctttacct tttaaattcc cttgattgtc ttgacttcat ctccagattc aaagccaatc
    23341 aggtaataat tcagtttccc atttgtttct tttcctacta cttcccacac aggctgatca
    23401 tcatacatcc ctaaagcagc tttttcgatt tggatttcag gatgggccgc agcaattgtc
    23461 tgttttgctt cgttttcagt cagtcccttt gtttgatcca atacttttac tttttcacca
    23521 gatttaggaa tgataacggc gatttctttt cctttatcat ttgtacctgt cagtgaaaag
    23581 tatgtcttct ttcgagtaaa ccaataaaaa tgatcggctt cctcaagatt tgcatatttc
    23641 tttgcgattt ctactgcttc tttcttcgcc tgcaccgccg gacgactgga acgcaagtaa
    23701 aaaatcgagc tgatcacgat gaccgctaat aaaaatacga tcaagccaag taacacgcga
    23761 ttaatcgttt tttcctttgt ctgttttttc ttcaagggtc gtcgctcctt tatcttcatt
    23821 ccttttcatt atagcagatt ttaattatga aaaaagacag ctcctctaac ttccttcttc
    23881 atttttcttt aagaattcat ggatgtcctg atagatttcc tccatagcta gctctttgat
    23941 cggtactttt ttcggtaatg catggatgat ccgtgcacca tattttgcag tgactagtct
    24001 tcgatccaaa ataagcaata cgccacgatc tgtctccgtc cttatcaagc gacctaatgc
    24061 ttggcgtaac ttcaaagctg ctttaggtac ggattcctgg taaaaaggat tgatcccctc
    24121 tgattctaaa taagcgttcc tagctttgac catcgggcga agcggattat cgaagggtaa
    24181 acgtgtgact acaacgatct gcaaagcatc cccaggcaaa tctacacctt cccaaaagct
    24241 gtcagcgcca aacaatacgc ttttttctga caaaatgaag cgtttcaaaa gcttctcccg
    24301 gcttccgcca aacccttgag ctagtatttc tcgtcctttt tctagcatag acagatgcat
    24361 tttctgatag accttctgca aaacatcatg agaagtgaat aacacaagta ttggccgatc
    24421 ttcttgttcg atcagacctg taagtgctgt ctctaaataa tcgatatatg cttgattgct
    24481 gacttgatgg acggcaggtg cctcttttgg cacatagatc cttgcctgtt tctcataatc
    24541 ataaggagag gcaatcactt taagaggtgc tgcttcaatg ccaagccgct tagagagata
    24601 gtgtcgatta gggccgatct tcagcgttcc tccaatataa agaatacgat catatcgagg
    24661 ataccaaaca gtcgacggca acatagccgc ttgaaaatca tggagatgaa aaatcatctg
    24721 ctgcgctgtg ttcccaggaa cgaaccaatg gatcaaatga tctcgccagt cttccagcca
    24781 ttgatccatc agcaagtact gcttgtctaa ttgatcaaat aagctatata aacttgctaa
    24841 atcgatccgc atctggtttg tccagctttc ttggataatc tcaaaagtca gacgcatttg
    24901 tgactgtagc tgctggatct cctgataaaa aagctgcagg tgatcaatgg ctgtcccaga
    24961 ttccagtggc agctgatcca acatctcttt tgtcatcatg acttcttctg gatactgtcc
    25021 gttaggttgc ttgccgctca ctaaactcat taatccttct gccatacaaa tctgtgcttc
    25081 gcatagcgca gaaagttctt ctcgataaat cgataaccag cgtaaggctt catgttcttt
    25141 cggtaaatag ctttgtattc ggtcaaacag ctgatttggt tcttctaatt gctggatttg
    25201 tttatggaaa tagttaatac ttaatcgttt gtcatttact ttttcagcga tttccggcaa
    25261 atgatgtact tcatcgatca acaaaaatgg actagctgga agcaatggtt cttttcgctg
    25321 tgtttcttgt attaaaaacg catgattgac aatcaaaaaa ttactttgcc tgattttctt
    25381 ttgcaagaaa cgaacaaagt cttgttcgta aaatgactga tttcttgcta aaaatccaat
    25441 acctctgtgc gcgatatctg caaagaatga atggttcaaa tttgttaaat gtaattcatc
    25501 caaatcaccg gtcttcgttt gcgtcagcca aactaagacc gccatttgat acaaagcata
    25561 ttgtttttgt tctggcggtt ccacaagtgt gcctttaaaa cgctgtaaat caatatagtg
    25621 gcgatagctt ttgataatta caggttgtat gggctgatct agcaattgat tcagcaatgg
    25681 aatgtctttt tctatgatct gctgctgcaa aactagcgaa acggtactga tgacagcggg
    25741 tttttcaggt gtagccaaat aactcaatgg cagcagataa ccgattgttt tacccattcc
    25801 tgtagctgct tcgatcatca aatcttttga ttcatctttt gtgaaatggt cgtagaccgc
    25861 attcatcagt ctgttctgtt cttttcgata catcagtgtt ttaccaaaca ttttttcttt
    25921 tgcttgtttt tttcttggat atgtccgctc gccataatgg acggatgtga atagttccac
    25981 ttcttttttc tgcaaagcta gcccatcgac gatttctaat gagggatcaa gtggctctgg
    26041 tttttctttc atttcttcaa tcacatgata aatgaaacga ctcgtatcca tacctgtgtg
    26101 tctacttaat cgcgcaatct gctctaacgt gatgattggc aattttctca tccgttcttc
    26161 gatcagcaaa agaagttgac ccgtcacttc ggcatcacta tctgcctgat gaggattctc
    26221 atgaataaat ccaaggcttt ctgacaaatc agctaagcga aagcttggtt ccgtcggcaa
    26281 gaaaatttgt gcaagttcta ctgtatcgat gcctggtatc ttcaaactag gaagtccgca
    26341 tcgcatgagt tcatggttca aaaaattata atcaaaataa atattatgcg cgacaaatat
    26401 ggtgtctgcc aaaagattgt aaatcgtttg ggcgacatct tcgaaataag gcgctttttg
    26461 gacgcgttga ttcgtgatcc ctgttaaatg ctggatttgt tttgaaatct tccgccctgg
    26521 atgaatatcg atcgaaaaac gtgaagtgat ccgacctgat tcaatcatga cgcagccaaa
    26581 ttggatgatc cgatcttctt ttggattcgt cccagtcgtt tcgatatcaa cgactgcata
    26641 agttcttggt ttcaaaatat cttcctcttc tcctgcctct aatgttcttt tgaaattata
    26701 ctacaaagtg cagaaaaata ctctcatcgc tttttaaaga aaatagacaa aaacgcccag
    26761 acacgcaaat gtctgggcgt ttatttttta gtataggtta attaagcttt agtaacgttt
    26821 gttgcttgag gtccgcgttg gccttcttca acatcgaaag ttactgcttg gccttcttct
    26881 aaagttttga atccgtcgcc ttggatagct gagaaatgaa cgaatacatc gtttccgttt
    26941 tctgcagtga tgaatccaaa tcctttttct gagttaaacc attttactgt acctgtttcc
    27001 ataataaaaa tcctccttgt gtatcaacac atattttgca attacttgaa tggtgtatat
    27061 ctggaagatg tttatttgaa acaagctacc acagattacc gaacaaaaac tactcctata
    27121 gtatatcata ccgtttacaa aaaagctaga gtttttcgac tttttttatt aaaaaaagtt
    27181 ttctcagcga ttacataaag tgaaaaaccg aacttttttt aaaataaacg acatattgtt
    27241 cagttggtgt ttttttgctc tgatgttctt ctttaaacta aaaaaattat tctttctgac
    27301 tacttatcgg ttctgtttca acggtcgagg ctggggaaga ttctttagta ggtctgatgt
    27361 tctcatggta tttctgatag cctatgacag ttaccagaag aacaaggcaa accaatacga
    27421 tcaacgagat tagtttttct tttttgctca tatctatacc tcataaataa caggatgatg
    27481 tccatcatat ttctgagtat gagtaaaagc aaaagctgca gctgtagaat gtaagaaatg
    27541 ccagcaactt tcataatcac aggtcggaca gtaccaaaag taaccaaatc ttggctgcaa
    27601 agaccgttct tctgtttcag gaatcgcaga atcaagatct gttgtctgta cataaacatt
    27661 tttgtacaga cgtgtgcctg taataggaga ccgctgtgtt tcttcagcaa aaactggctg
    27721 acttgttaaa cttagggata gaaacagcaa acaccataac agtctttttt tcataaagac
    27781 ctccttatcg ataagttttt cccaattaaa atcatatctc aagaacgccc gttattcaca
    27841 ttttgttcat aaatgatgtt ttttgtatca taatcgtcaa aaagaggtcg tgaccacgtc
    27901 acaacctctt ttt
//
LOCUS       DACKAC010000036        26526 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.036, whole genome shotgun sequence.
ACCESSION   DACKAC010000036 DACKAC010000000
VERSION     DACKAC010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.036"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            8..850
                     /locus_tag="I0B46_001572"
     CDS             8..850
                     /locus_tag="I0B46_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAR2083879.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            847..1797
                     /locus_tag="I0B46_001573"
     CDS             847..1797
                     /locus_tag="I0B46_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAR2083880.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            1812..2045
                     /locus_tag="I0B46_001574"
     CDS             1812..2045
                     /locus_tag="I0B46_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAR2083881.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            2376..2960
                     /locus_tag="I0B46_001575"
     CDS             2376..2960
                     /locus_tag="I0B46_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAR2083882.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            3091..3759
                     /locus_tag="I0B46_001576"
     CDS             3091..3759
                     /locus_tag="I0B46_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAR2083883.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            3761..5185
                     /locus_tag="I0B46_001577"
     CDS             3761..5185
                     /locus_tag="I0B46_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAR2083884.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQNPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVK
                     NRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDIN
                     FDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQF
                     FTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSK
                     TNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGG
                     IKKDIKLRVEDSTLIEQIFNDITK"
     gene            5203..5772
                     /locus_tag="I0B46_001578"
     CDS             5203..5772
                     /locus_tag="I0B46_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAR2083885.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVVSWVLMQLGSAAGQWIPIWFFTDRFRQDWIDRGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            5882..7636
                     /locus_tag="I0B46_001579"
     CDS             5882..7636
                     /locus_tag="I0B46_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAR2083886.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQYLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHRVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            7590..9362
                     /locus_tag="I0B46_001580"
     CDS             7590..9362
                     /locus_tag="I0B46_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAR2083887.1"
                     /translation="MRMQMIGGLTMKNFYKKKFALTDQGAEALTKASISSFFVYCINM
                     VPAFIIMMLIDELVLENAKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     VQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            9885..10154
                     /gene="rpsN"
                     /locus_tag="I0B46_001581"
     CDS             9885..10154
                     /gene="rpsN"
                     /locus_tag="I0B46_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAR2083888.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            10169..10348
                     /gene="rpmF"
                     /locus_tag="I0B46_001582"
     CDS             10169..10348
                     /gene="rpmF"
                     /locus_tag="I0B46_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAR2083889.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            10380..10757
                     /locus_tag="I0B46_001583"
     CDS             10380..10757
                     /locus_tag="I0B46_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083890.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            10778..10927
                     /gene="rpmG"
                     /locus_tag="I0B46_001584"
     CDS             10778..10927
                     /gene="rpmG"
                     /locus_tag="I0B46_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAR2083891.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            10951..11934
                     /locus_tag="I0B46_001585"
     CDS             10951..11934
                     /locus_tag="I0B46_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAR2083892.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            11964..12086
                     /locus_tag="I0B46_001586"
     CDS             11964..12086
                     /locus_tag="I0B46_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAR2083893.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            12104..13627
                     /locus_tag="I0B46_001587"
     CDS             12104..13627
                     /locus_tag="I0B46_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAR2083894.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            13651..13890
                     /locus_tag="I0B46_001588"
     CDS             13651..13890
                     /locus_tag="I0B46_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083895.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(14083..14544)
                     /locus_tag="I0B46_001589"
     CDS             complement(14083..14544)
                     /locus_tag="I0B46_001589"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAR2083896.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(14739..14957)
                     /locus_tag="I0B46_001590"
     CDS             complement(14739..14957)
                     /locus_tag="I0B46_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAR2083897.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            15075..16691
                     /locus_tag="I0B46_001591"
     CDS             15075..16691
                     /locus_tag="I0B46_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAR2083898.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(16932..18272)
                     /locus_tag="I0B46_001592"
                     /pseudo
     CDS             complement(16932..18272)
                     /locus_tag="I0B46_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(18548..18703)
                     /locus_tag="I0B46_001593"
     CDS             complement(18548..18703)
                     /locus_tag="I0B46_001593"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAR2083899.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(18999..19331)
                     /locus_tag="I0B46_001594"
     CDS             complement(18999..19331)
                     /locus_tag="I0B46_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAR2083900.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(19845..20036)
                     /locus_tag="I0B46_001595"
     CDS             complement(19845..20036)
                     /locus_tag="I0B46_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAR2083901.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            complement(20130..20516)
                     /locus_tag="I0B46_001596"
     CDS             complement(20130..20516)
                     /locus_tag="I0B46_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAR2083902.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            21210..22415
                     /locus_tag="I0B46_001597"
     CDS             21210..22415
                     /locus_tag="I0B46_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAR2083903.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            22432..22866
                     /locus_tag="I0B46_001598"
     CDS             22432..22866
                     /locus_tag="I0B46_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083904.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            23483..>26526
                     /locus_tag="I0B46_001599"
     CDS             23483..>26526
                     /locus_tag="I0B46_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297336.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HAR2083905.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAGEGTSIESGTTVFAVKDGVSLPE
                     DKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATKLDDIIENPGENIPAGYHKVT
                     FTAGEGTSIESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKWPEEATQPITADDTEF
                     VSSATKLDDKSDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEKVDVS
                     AAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT         9185 a         3815 c         4994 g         8532 t
ORIGIN      
        1 gaataatatg cttcaaaatt ttattaatgg tctgtatgac tttcactttt tacagaatgc
       61 tctaattaca tctgtagtta tagggattgt ttctggaaca gtaggttgtt ttattatttt
      121 aagaggaatg tctcttatgg gtgatgctat ttctcatgct gttttgccag gagtagcaat
      181 ttcttatatt ttaggtatta atttttttat tggtgcgatt gtatttggtc tattagcttc
      241 tatcattatt acttttataa aaagcaatag tgtaataaaa ggagatacag caattggtat
      301 tactttcagt tcttttttag cattaggagt tatccttata ggagttgcaa atagctcaac
      361 ggatttattt catatcttat ttggaaatat tttagctgta caagatattg ataagtggat
      421 tacaattggc gtttctgttt tagtgttggt tacagttgtt ctatttttta aagaattatt
      481 aatcacttct tttgatccat taatggcaaa agcaatgggg atgaatgtca gtttttatca
      541 ttatctatta atgattctat taactttagt agcagtaaca gctatgcaaa gtgtgggaac
      601 tatattaatt gttgcaatgt taattacacc agctgctaca gcatatttat atgcaaacag
      661 cttaaaaact atgatattta tttcggcgac agtcggagcg tgctcatcat tgctaggttt
      721 gttcattggt tatagtttta acatagcagc aggatcaagt attgttttaa cagcggcatt
      781 cttatttgta attagttttc taatctcacc aaaacaaaaa tttttaagaa agaaggagag
      841 aaagttatga tgagaaaatg gaaagcagta ctgggaagtc tggggatttt gattgctctt
      901 tttatattcg gggcatgttc aacaaatagt aaagacaaag atacagtggc ttcgaacgaa
      961 aaattaaagg tagtagttac taattcgatt ttagcagata ttactgaaaa tatagcaaaa
     1021 gataaaattg atttacacag tatcgtacct attgggaaag atccccacga atatgaacct
     1081 ttgcctgaag atgttcaaaa aacttcaaaa gcagatttga ttttttataa cggtgttaac
     1141 ttggaaactg gaggaaatgc ttggtttaca aaattagtaa aaaatgcgaa caaagaggaa
     1201 aacaaagact attttgcagc aagtgatggc atagatgtta tttacttaga gggtcagagt
     1261 gagaaaggga aggaagatcc ccatgcttgg ttaaatttag aaaacggtat tatttacgct
     1321 aaaaatattg aaaaacagtt agcggaaaaa gatcctgata ataaaaaatt ctataaagaa
     1381 aatctagata agtatattga aaaattggat tctctagaca aagaagctaa atctaaattt
     1441 gcttcaattc cgaatgataa aaaaatgatt gttacaagtg aaggatgctt taaatatttc
     1501 tcgaaagcgt ataatgtgcc ttctgcttac atttgggaaa tcaacactga agaagaagga
     1561 acaccagatc aaataaaaca cttagttgaa aaattacgca caacaaaagt tccctcctta
     1621 ttcgtagaaa gcagtgtgga cgatagacca atgaaaacag tatcaaaaga taccaatatt
     1681 cctatctatt caacgatttt tactgattca attgcagaaa aaggacaaga tggtgatagt
     1741 tactatgcga tgatgaaatg gaacctggat aaaattgctg aaggcctttc gaaataattt
     1801 ggagggtagg tatggtacaa attaaaacga ctactatact aatttattta ctagcaattc
     1861 aggaattatc aaaaagaaaa aagaaattaa aaaataatga tttagcaaaa gttctaagtg
     1921 ttagtccagc atctgtaagt gagatgctgg ctaaacttag atatgaggac tatttagata
     1981 taggatttca attaactgat aaagggaaaa aatttattga cgattacaaa aaaggtttta
     2041 agtaaaagac tttattttta gggaggataa gaaaatggct attggagtga ttatgatagg
     2101 acttacccta atagctggat ataatttttt taaatctttc tcggattatt ttagaaaagg
     2161 tgaatgattt agatgagtct attaattctt gtaggattgc tttttgtatc agttattttg
     2221 ctctttctca ttagtttagc tctaaggaat gagttcagta atagacgtta acgattgaaa
     2281 cttcaagtcc tgtgtaagat aatatagtta attcatctta ttaggaggaa gtacccatag
     2341 aaaccagaga ttatattaca actcaccaaa aattaataga aagtggacta agccattttg
     2401 aaaaagaagg atttcagcaa actaagataa ggcaactgtg cttgaccgca ggcgtaacta
     2461 cgggtgcttt ttataaacat tttacttcta aggaagcgtt atttgaagaa attattcagc
     2521 cgcatattaa tgcgcttatt aatatgtata atgaagagtt taccaaatac ctcttaaact
     2581 tagaaaatat agatatagag aaaagttgga gagagaactt caatgaatta gatccattta
     2641 ttaattatat ttttgagaat aaacgtatat tcgatttggt tttattccaa tcagctggaa
     2701 ccaaatatca agatattgtt gaaaaaatta catcatttgt gacaaaaaag acagtctgtt
     2761 gtctagataa gttaaagact atcggaatga tcaatcaaaa tttagtcttt aatgaagaag
     2821 aaatccactt ctacatttac tcattctacg ctacattcta tgatatattg aagcatagct
     2881 attcaaaaga aaagactatg ctattaacca gaagattgta tcaattttct gttcctgggt
     2941 ggattgaatt actgagttaa ttaaaaaaaa gcttgcattg agaattgttc tcagttatgc
     3001 tttttgataa gatcgcactg ttatctgaat aacggtgcat ttttgtcagc ttaagataac
     3061 agtgcgatct tattttttgg aggtgaaaga gtgtcaaaaa tcaatccgtt aaccttgatt
     3121 actttaaata ttttttttcc ggtcattatt tttttaggaa agggaaatat atttcaagtg
     3181 gggtgtttca ttattgctac agttttagta ttgatgtttc gtaaaataaa gacatgtatt
     3241 tatttcatct tgatttattc agttctgttt ttagtaatga aaattattga tgctatcaag
     3301 atagagtttt ttactatatt ttttgggaca tccttatata ttgtatttcg aatgttgccg
     3361 gtcgtaatga tctcatggtt gttggttagt gtctatacta gcaatgaatt attatcctct
     3421 ttagaaaaaa tacatttacc gaaaaaaata atgttaagta ttacagtagt gatcagattc
     3481 tttccgactt atcgttctga aataagaatg attaaagaat ctttaaaaat gagaaacatt
     3541 cgcttgtctt tttttcagcc tctgcaatat ttggaatttc tgttagttcc cgttcttata
     3601 cgtgcaacta tgattgctga agaaatgacc gctaatgcaa taacaaaggg gatagaatct
     3661 cctgtgaaaa gaagttctta ctataaaaca aacatgagct tattagacta tatcatcctg
     3721 ttttccgtta ctattttttt cttaagtatt ttgatttgag gtgaaagaat tgtttgaatt
     3781 taacaatgtt tctttttcat ataaggatgt taaggaacaa gcgcttgttg accttaattt
     3841 ttcaataaaa aaaggcagtc ttacattgat tactggttct tctgggagtg ggaaatccac
     3901 actattaaag ttaatgaatg gacttattcc aagactttat gacggaaaat tggttgggga
     3961 aattttattt gaaaataact cgctacaatc cttaacacaa gaaagtatat caaaaaatat
     4021 tggctacgta agtcaaaatc ctagaggaca attctttaca acaaatacaa cgagtgagtt
     4081 agtattttcg atggaaaact ttggatttac aaaagagcat atggataaaa gaatcaatga
     4141 attagctgaa ttattagatc tttcaaatat attagataaa aacatttttt cgctatcaag
     4201 tggtgaacgg cagaaaattt ctattggatg tagtttatcg ctaaactcta aattaattat
     4261 attggatgaa ccgtcttcaa atttggactt caaagcgact aagaaacttg ctgacttact
     4321 ttgtgattta aaaaataaag gatttactat tgttatagct gaacatcgtt tttactattt
     4381 gaaagaatta attgatcaag ttctatttgt taaaaataga actgtaaaaa ctacaactat
     4441 tcagttgtta aaaactgaaa acaataacgc attaaggaaa tttgacattt ttaatagttc
     4501 gattgaagtt ccctatatcg aaaaggtaag tgcagatgta ttaaaagttg aagaaattac
     4561 ttatgaaaat attctaaaag acataaattt tgatgtcaaa gttggagatg ttattggttt
     4621 ggtagggaaa aacggtgtgg ggaaaacaac cttactaaga atactcatga agattattcg
     4681 ccctaccaaa ggaaagataa tcgaaaataa aaaaaatttg tcatcgccgt ttttagtaat
     4741 gcaagaaatg gattaccaat ttttcacaga atcagtgagg agcgagttag ccctggggaa
     4801 tgaaatagtt agtaaagatc agcaagaaaa aattttaaaa aaaatggaac tttataaaat
     4861 gaaagatcaa ttaccgtttg atttatccgg tggagaaaaa caaagattat tggtttctat
     4921 agcttcattg tcaaaaacta atttatttct atttgatgaa cctacaagtg ggttggatta
     4981 tatcaatatg gaaaggattg ctgaccttat taaagactta aaagaaaagg gtgcagtagt
     5041 tatagccact catgatccgg aatttcttta taaaacatgc aatagaatta tttatttaaa
     5101 aaatggtgga ataaaaaaag atataaaact tagagtagaa gattcaactt tgatagaaca
     5161 gatttttaat gacataacga aatagaattg aggagaatat atatgaaaac aaaagattat
     5221 atttttattg gtttagctac agttatttcc ctagtaattt actttttttc aataatggtt
     5281 agtagtattg gtggggcgtt tggtcacagc atatccccag ggatttttgg attattatct
     5341 ggaatcatat ttgtttatat tagctataac taccctagaa aaggaatttt tactatttac
     5401 acaattgttt tattgttatt ttttacgtta atgggcgggg cgtacttacc ttggtttatc
     5461 agttcgatta gtatggcgat cttagctgat attattctat ctgtatttgg atatgatcga
     5521 gcaattcctc aagttgtgtc ttgggttcta atgcaactag gcagtgctgc cggacagtgg
     5581 attcccatat ggttctttac agatcgtttt cgtcaagact ggatagatag aggacaatct
     5641 gctgcaacaa tggatgcaat gatccactac gcggtgggta tatggggaat tatttcagtg
     5701 ttagttgtag caagtttatc tatgattggt gtattgattg gaagaaaagt cttaaaaaaa
     5761 tacaaaaaat agaacgtagt ttaccttcta tctgtttgta catctctaat ttacgctatt
     5821 acggaaagca ttaacagttg taaacgaata agagaagaaa ttgaatgcgg gggaggaaaa
     5881 aatgaaagta tacaaaaaat tatttagtta tgtccctaaa atgaaatact acggatggtt
     5941 agccacttta ttttctgatt tttctgtcgt attaacagtc tatggatatt acagtattta
     6001 caaatttctt cagtctctaa ttattgtagg agatgaatat ttagctaaga gttatgcggt
     6061 tcaaactgtt gcttggttaa ctttaggagc tttattttat attttttctg gcttattttc
     6121 tcatatcctt gggtttcgct tagagactaa tttgagaaaa aagggaatta gcggtttaac
     6181 agaagcaagt tttcgttttt ttgatttaaa ttcttcggga tatattcgaa agacaattga
     6241 tgacaacgcg gctaaaacac atatggcaat tgctcacttg attccagata gtgctcaagc
     6301 catagtaaca cctgttttat cagttgcctt aggctttttc attaacttta aaattggatt
     6361 ggttctaatc gccatgttag taattggtgt attcttgtta aagaaaatga cagagaatac
     6421 aaattttatt caaaaatatc aagaatcgtt agatgtgtta agctctgaaa cggtagaata
     6481 tatccgcgga attcaagtta taaagatatt tggtgctaaa gtaacatcat ttaaagcctt
     6541 gaataaagca attaacgatt atgctaagtt tgcatataag tattctttga gtgggaaaac
     6601 accttttgtt ctttttcaat ggctattttt tggcatagtt tgcattttga tagttcctat
     6661 aacctttttt ataacttctc ttaccactcc caagatctta gctgttgact tgttgatgtt
     6721 gtttttttta agtggaatta tttttgtttc tttcatgaga ataatgtatg taagtatgca
     6781 cttgtttgaa gcaagatatg cagtcgaaac actagactcc ctttatttag aaatgaagca
     6841 agaccaacta aaatatggga acgagacaca gattaaaaat tgtgacattg catttgaaaa
     6901 tgtcagcttt tcatacaaag aagagtgtgt tttagaaaat ttcaacttag ttttagaaca
     6961 aaaaagaatt tatgcattag taggaaaatc aggaagcgga aagagtacga tagctaaact
     7021 aatttcaggt ttctacaatg tggataaggg acagattaaa attggtggta agcctttaaa
     7081 tcactattca aaagacacaa ttattaacac aattgctttt gtctttcaag atacgaaact
     7141 attcaatcag agtatttacg ataacgtttc tatagcaaat cctcaagcgc cagcaagtaa
     7201 agtatttgac gcgctaagat tagccggttg cgaatcgatt ttagaaaaat ttcccgaaag
     7261 agagcatacc ttaattggta gtaaaggagt ttatctgtca ggaggagaaa aacaacgtat
     7321 agctattgcc agagcaattt taaaagatgc aaagattgtt attatggatg aagccagtgc
     7381 atctattgat ccagataatg agtatgaact acaaaaggct tttcaatatt tgatgaaaga
     7441 aaaaacagtt attatgatag ctcatcgatt atccagcatt aaatctgtag atgaaattct
     7501 tgtcttgtca gaaggaaaaa tagtagaacg tggaaatcat aatgaattaa tggctaaaca
     7561 tcgagtgtat aaacaattgg tcactaatta tgagaatgca aatgattgga ggcttgacaa
     7621 tgaaaaattt ttataaaaaa aaatttgctt taactgatca gggcgcagaa gctttaacta
     7681 aagcttctat ttctagtttt tttgtatact gcattaatat ggtaccagca ttcattataa
     7741 tgatgctgat agacgaatta gttttagaaa atgcaaagcc ccgttggctt tattttgcag
     7801 tatcgtttgt tactttgctt ttcatgtatt ggttgttaga tagggagtat gaaaacttat
     7861 ataatagtac ttataaagaa agtgcgcatt taagagtgca aattgcagac gatttgtcaa
     7921 atttaccatt atcctatttt tcaaaacata atttatcaga tttatctcaa actatcatgt
     7981 ctgacgttga aggtattgag catgcgatga gtcatgcaat acctaaagcc ggcggtatgg
     8041 ctctgttttt cccttttatt tcagtgatgc ttttggttgg taatgtcaaa atgggattag
     8101 ctgttatttt gccaacgtta tttagttttg tcttaatctt gttatcaaag aaatcccaaa
     8161 cgaaagccaa tactaaatat tacgatactt tgagagaaaa ctcggaagaa tttcaagaaa
     8221 ctattgaatt gcagcaagag attaatagct ttaatctatc taaaaaagtt caagacagac
     8281 ttttcaaaaa aatggaagag agtgaaagga ttcatttaaa ggtagaatta agtacttttt
     8341 cagtcatggc cttatcctct attttctcat atgttagttt agcagtagtc attctagtag
     8401 gtgttcactt actgttaacg ggtgaagtga ctatactcta cgtcgttggt tacttactag
     8461 ccgcaataaa aataaaggat tccttcgatt ctatgaaaga agcagtgctt gaaatatttt
     8521 atttagctcc taaaatacag cgaattagag ctatgaagga aaccagtatc caagagggat
     8581 cagatagtcc tctgaaatct tttgacattg agttgcgaga tgtctctttc tcatatgata
     8641 acaatacccc aattttagat catatctctt ttacggctaa acaaggagaa gtgactgcgt
     8701 tagtaggtgc tagtggttcc gggaaaacta gtatcttgaa attagtgtct agactgtatg
     8761 attatgatga aggctgtatt ttaattgatg ggtatgatat taaacgtgta tctccagctt
     8821 ctttgtttag caaaatcgct attgtttttc aagaggtaac tttgtttaat acaagtattt
     8881 tggaaaatat acgtattgga aatagtcaag cgtcagatga ggaagtgaaa aaagcagctc
     8941 gtttagctaa ctgcgaagac tttattgaaa aactacctga cggttatcat acattggtcg
     9001 gagaaaacgg aagttcactt tctggaggag agcgacaacg cctatccata gcacgggctt
     9061 ttttgaaaaa tgcgccaatt ttaattttag atgaaattac tgccagttta gatgcggaaa
     9121 atgaaaaacg tattcaggaa agtttaaatc gtttaataca agataagaca gtactaatta
     9181 tatcgcatcg cttgaagtct attgaaaagg taaataaaat tgtagtgatg gatcaaggaa
     9241 aactagttga tcaaggtact cataatgagt tgtatcgaag gtcagaaata tacaaaaatc
     9301 tcataaagaa aactaaacta tctgagaaat tcgtctatga aaaagaagct caatcacgat
     9361 gatgaaatag gctttaaatt aacctacaat ttttgggata tagttataat tctttttcaa
     9421 aaagaataat tttattaaac agcatctaat tttgaataca cgaaagaatc agtatgattg
     9481 aattagttca cttaatgtct attcttataa tataaggaat aatcaaagtt ttaaaaaatt
     9541 ggcgataaac cagttaaaca taaaagattg aataaaaagt tatagtttct ttcatattgc
     9601 cgttttaagt agaggaaaag ctacttaaaa cggtatttgt gtttgattgg actaataaaa
     9661 gtgaattctt atttttcaga gcctaaactg catattcttt taaatgattt gattatctct
     9721 atgtaccgat aaattcgttt tcaaatagtt atctattcat atgagtttta tttcatgtta
     9781 tttattgttg acaagagcaa ctcttgttgg taaagtatca gtgtcaaaac gtaacgatta
     9841 cgatttgagg tgtctaaatc aaaataaaag gagtataaag gtatatggca aaaaagtcga
     9901 aaattgctaa agcaaagaaa caaatggcga tgattgaaaa gtatgcagat aaacgtcagg
     9961 aattaaaagc cgcaggagat cgcaccgctc tcgctaaatt gccaagagat tcaaatccga
    10021 atcgtctgag gcttcgtgat cagacggatg gtcgtccaag agggtatatg cgaaagttcg
    10081 gtatgtcccg tattaagttt agagaacttg cccatcaagg gttgattcct ggagtaaaaa
    10141 aagcaagttg gtaaaaggag gaaatatcat ggcagtacca gcaagaaaaa catctaaggc
    10201 gaagaaacgg ttaagaagaa ctcatcaaac tgtagtaaaa ccagaaattt catttgacga
    10261 agcgaatggt gattatagac gtagccatca tgtatcttta aaagggtact ataatggaaa
    10321 gaaagtaatt aaaggagctt caaaatagtt taatatataa cttttaggag ggaatcaaaa
    10381 tggaaattat ttacccacct ttagttgaag aaggattaaa gtattatctt gaaacgaccc
    10441 agcaatcgct agataaatgc actttttatc gttcgatggt agagagagga attattactg
    10501 aaacgggttt accgacccaa caggcaattg aaaatggatt agtcaaagat tactatgagg
    10561 accaaggctt atcatttgat gattttttaa gaatctatcc aatttttgaa gagtatgacg
    10621 aggagttatt tcaatgtatc gatggttatt gggagatacc tgttgatatg aaagaaaatt
    10681 tagtttcaca attggaatct ggcgagttaa cttttgaaga tgcacaacaa attcaagcat
    10741 atcttgaaga tcgataagta ttgaaggagg aagatttatg cgtcaaaata ttattttgga
    10801 atgtgtcgaa actggagaac gtctgtatct caccagcaaa aataaacgga ataaccctga
    10861 acggttagaa ttaaaaaagt attcgccaaa attgcgcaga cgggcaattt ttaaagaagt
    10921 gaaatagcta agtggtaaaa aggtgacaaa atgggcatac cagttacaat cgtttctggg
    10981 tttttagggg ctggaaaaac aacgctaatc aatcaagcct tgcaagtctc accctacccc
    11041 agggaagaaa ttattataat tgaaaatgag tttggtgaag ttgggattga tcacaaatta
    11101 ttagttcata gcgaggaacg ggtgttacaa ctaaataatg gatgtatgtg ttgcagtctt
    11161 cgcagtgatt tattagcaat attgatttct ttactggaaa caatagaaga acatcaacaa
    11221 ccaatttcac aaatcatcat tgaaactaca ggtatagcag atccccaacc aatcattcaa
    11281 acacttttaa cagcgcctca actaagagga agagtatata tcgatagttt attgactgtt
    11341 attgatagtg acagttttga acttattgaa agagaaccac aagcccttaa gcaacttgtc
    11401 atggcagatc gcatttttgt gtctagaaaa caaaacttga atttatctgc actaaaaaaa
    11461 ttacagagag aaatcaaaag tattaaccct ttatccgata ttcgaatgtt cacacatagt
    11521 gaaacgattc aaaaaaagga tttttataat ttgaaaaaat ttaatcatcc cttagttttt
    11581 gaaagtgatt catcagaaaa tcactataat ggcaaatact atcatcaaca ccatcaagta
    11641 catgagttta aatcaattct cttaacttca gatcaagaca taaaaaaaga ttttttactc
    11701 caatggattg actggctgat tttaaccaac caagggagcc tgtatcgttt taaaggattg
    11761 attgctttac aagagcagga ttttatagtt gccatgcaag aggtaaacga acaggttaat
    11821 tttcaataca ccactcgaca aaaagaccaa accactatta ttctaatcgg aaaaatgttg
    11881 gatgaagaaa ggatcaaaca gagttttgat aaattactgg cggaaagcaa ataatgataa
    11941 gtagtgattg ggggtaattt ctaatggaga aaacggatat atctagtgca tatcgacgac
    12001 ttaaaagccc gaatattaaa actagaaaaa gggcattaaa aattatcaaa gaacataaac
    12061 gaaataaaat gaagaaatta gcataattaa ggaaggaaaa agaatgaaga aaatcagtgt
    12121 aggacttatt gggatagcag ctctaggatt attgggagct tgctccagca ctaatgatgc
    12181 aaaagtatcg aatgataaag atggaaaatt agaaattgta acaacattct atccaatgta
    12241 tgatttcacc aaaaacatcg tcggcgatga agcgaacgtg gatttaatgg ttccagcggg
    12301 atcagaacct catgactatg agccctcagc taaggatatg gcaaaagctc atgatgctga
    12361 tgtttttgta tatcataacg aaaacatgga atcatgggta ccaaaagcta aagaaagttg
    12421 gaagaaagct gggcctaatg tagtagaagg aacaaaagac atgattttat tgccaggtag
    12481 tgaagaagaa gatcatgatc acggagaaga agatcaccat catgaactag atcctcatac
    12541 gtgggtttca cccaaaatgg caattaagga agtttcaaat atcaaggatc agttagttaa
    12601 attgtatccg aaaaaagcaa aagtatttga gacaaatgct gaaaagtatt tgactaaact
    12661 gaaacggttg gatgctgact ataccactag tcttaaagaa gcgaaacaaa aaagttttgt
    12721 gacgcaacac gcagcctttg gatatttagc tttggattat ggattgaaac aagttcctat
    12781 tgctggacta agtcctgaag aagagccatc ttctggtcga ttagctgaat taaaagagta
    12841 tgttaaaaaa aataagatca attatattta ctttgaaaag aatgctaatg ataaaattgc
    12901 aaaaactttg gccaacgaag ctgggataaa attggaagta ctgaatcctc ttgaaagtct
    12961 tacaaaagaa caaatggata atggtgaaga ttatgtttca gtaatggaag acaatctaaa
    13021 agcgttagaa aaaacaacta tggttgctgg tgaagaggta gttccagaaa aagaagcgaa
    13081 ggatgaaaag acagttgcta atgggtactt taaagatgcc gatgtgaagg atcgcgaatt
    13141 gagtgattat acaggcgaat ggcaatctgt ttatccgttg ttaaaagatg gaattttgga
    13201 tgaagttttc gattacaaag caaaaatcaa taaagacatg acagctgccg agtacaaaga
    13261 ttattatact actggctata agacagatat tgatactatc aacattaaag acaacaccat
    13321 tgattttgta gtcaacggtg aacatcatca atacacatac aaatatgttg gatacaaaat
    13381 tttgaattat gaaaaaggaa accgtggtgt tcgttttaat tttgaaactg atgatgcagg
    13441 tgctggtcgt tttaaatata ttcaatttag tgatcatgga attgcaccaa gtaaggcaga
    13501 gcatttccat attttctttg gtggagaaag tcaagaaaaa ctctataatg aattgcataa
    13561 ttggcctact ttctatccag cttcattgtc taaacatgaa attgctcaag aaatgatggc
    13621 tcattaaaat tcaatttttg gaggaaatac atggtaagaa aagtattatt gtctatggat
    13681 tccaagaata ttgatgctta tcggcgttat cgatttgcaa agcaaatgag attaaatggg
    13741 agtacattag ctgatattgc tgcaacttta catgttagta ttgacacagt tcatagagta
    13801 ttaaaacggg atcctgattg ttgttttatc aaggtcaata ctggaagaag tagacgttta
    13861 gcaaaacggc ataatagctt ggccaattaa ttgataatac cagagtagat catttttccc
    13921 ttatttggat ttgatctgct ttttattatg tcttgaattg tcaaatcaag cgcaacactt
    13981 tatcaaagaa gacttcataa ggtgttctcc aatctaaaca ctttctaggt ctgttattca
    14041 gtttttcaat catctgattt atcgttgagt tatctaccgt agctatctct ttcgattttg
    14101 gcaagtattc tcgaatgagt ctattcgtat tttaatttgt tcattgttgc caagggcatg
    14161 aggatctgga aaataaaagg gcatattatt caatgcttct gtaactgcga aatgattgga
    14221 gaattctttt cctctatctg gagtgatcgt tttgaccgcc tcatttggaa tactgcttaa
    14281 aagttcaatc attttctcgc tcacataagc ggctttcttt ctggggacct tctttcctgc
    14341 taaaaggaag catgattttc tgtctgtcaa agtcactaaa caagtaccac cagttttgcc
    14401 agctacaata tcggctttcc aatgaccaaa atcacttcta tcatctgcgg attttggacg
    14461 gccatggatc gaatgagtaa ggcaaatctt tccacgagac tcgacatgat tttttgtatg
    14521 tcttgttttt cctcgatggt gcaatgaacg gatcaggcct ttattgccgt gtgagagggg
    14581 ttctgtatcg aaaagatcat tgcaaattgc acgttagatc gtgataaaac taattgaaaa
    14641 gtccatatgt tcatgcttca aacgattcga gatttcctct ggcgaccact gaaaatcgac
    14701 aaataaatga cgaacaattg accaaactca cggatcattt agaatgtgat aacgtccgca
    14761 tttttttctt cgataagcat aatttttttc cgctttggaa ggtgaatagt ttttttcttg
    14821 tttcttctca gttcatgtaa aatagtaaac gggctatgct gaatgtcgtt gccaattata
    14881 ctaatagaaa atccttcttc gtgaagcaga aagattcttt ctcgttcgag tatagtaaga
    14941 tgacgatact ggctcataat tattttcctt ctaggagtgt tctcgacaaa cttatactag
    15001 ttggatatcg attatgactc atttttatgt gttgcactta aagtgtaaat tcaaggtata
    15061 gaaagaggtt ccctatgaaa aaaataccta ttacaatctt aactggctat cttggttcag
    15121 gaaaaacgat acttctaaat catattctgt acggtgatca cggaaaggaa attgctgtca
    15181 ttgtaaatga cataggggaa gtaaatgttg atggggtatt agtcgacaag aaaggtttta
    15241 aaagaactgg agaaaaactt gtttcgatgc cgaatggatg catttgttgt acattacgcg
    15301 aagatttgat tgttgaacta gatcaattag tgcaattttc tgacttgaat caaattgtta
    15361 ttgaagcttc aggaatcagc aaaccaattc caattattca agcaattata ttagctaaaa
    15421 accctttaga cattgatttg acccaacgat tagagattgg tgccattatt atagtagtgg
    15481 atgtaaaacg tttttgggat aatttcgaaa cgggtgaaaa gattcaggaa agaaaagctg
    15541 aaggtaatgt agttgaagaa gaacaggata ttcgtggctt gttaattgat caattcgagt
    15601 gttgtaacat attactttta aataatagtg acttagtgtc aaccaagacg atttacaaaa
    15661 ttaacggtct aattgctaag ttacagtcca atgccaaaat aattaggaca attaaaggtg
    15721 aagtagactt atcgaaaatt ctaaatacaa atctttttaa tatagatgag acttcctatt
    15781 cagtgggctg gttcaaagag ttagagcttg gctatcaaaa tcatattcct gaaacagaag
    15841 aatatggtat ctcaagtttt atctatcgaa atcggttact ccttgacgca caaaaatttg
    15901 cccatcttac ggataaaaat tttcctggca atattacaag agcaaaaggg tttctttggt
    15961 ttgcccaaca tagtgatttt tcgacgatct ttgaaacatc gggtagctat aaaagtattg
    16021 aatcattaaa ttattgggta gctagtttac ctaaaattga gtgtcaaatg attatacaaa
    16081 aaaatcccga attgaaggaa gcctggcatc cgttgtttgg tgaacgcatt aataaactag
    16141 tttttattgg tatgaagatg gatcaacaat gtattaagga acaacttgag ggttgtttaa
    16201 ctgattctct taatgcagat aatttccgaa aaatttcacc atttccatgg aaaaagtcta
    16261 atatgtttag ccagatatcg ttaatgaaat ttttaagaaa agctggtgtc aagataggcg
    16321 atgtcataat tattggcatt ttgattatta cctcttttat tcccttattt gtattctcta
    16381 tagaaaagga gaatgaaaca actgaagatg ctatcaaata tgccatcgtt cgaattgatg
    16441 gcaaggaaat ggatcgcttc gatttggata agatcaatca taaactcgtg aattatcatc
    16501 ctgcgaaggg acaatataat attgttgaga ttaaagatgg aagaattcga gttaaagaag
    16561 ataatagtcc tgatcaaatc gcggttagaa ctggatggat ctcaaaaccc ggtcaaacga
    16621 gtatttgttt accccataag ttagttatta gtattgagaa aaaagaaagc acagattact
    16681 acatttacta atctggaaat agatgaggag gtttttaata agaaaaagat attattatta
    16741 ataatagcag gggctttatt aagtgtgtta agcgcttgtg gacaagaaaa agaagcggtg
    16801 acaacaggca caagtatctt taacgggacc tgcaatggtt tagttaacag tcggttagat
    16861 tacttatttt ggctcttcgt caatgagtgt tggtgaagaa attcaataga ataatcgaga
    16921 tactcacacg gctagacagc tatcttgtct agccgtgttg ttgttctggc tttagtcagt
    16981 tgggtagtat tcttagtttt cttaaaatat tttcctcgat ataaaaagat tcgttctcgg
    17041 tagttttgaa agtttctata gccataggca attcgtttta gtactttgat atggttattc
    17101 aaacattcta tagggccgtt ggaataggga acgcgcaacg ctcgtttgat ttctgtttgg
    17161 tatttcttga aggtcgtgaa cactggttgg tagtgctctg gcaactcctt gtagtcttct
    17221 tttaggagcg cgacgaattc aggaacatct tgtcttctga taacggaaag aaaatcctgg
    17281 tagacttcgt agcctcgctt taaggaatca tcataagata atagccgatc gactatctct
    17341 gcttctgtca gatgatctcg aaatgacgga cgccagtagc gatgctgacc tttcaattcc
    17401 cacgcattct tttgaagtag tttccagtat ttctttaagt gcttcgaccg acgatcccct
    17461 tttttaaagg tcttcattac ttgaattcgg tggttggtaa aagcccgacc aatgtgttgg
    17521 ctaatatgaa agcgatcggc gacgattcgg gcattcggaa aacactcttt caccaatgtg
    17581 cgatagggtg tatagaaatc tgaaaccact agacgcactt gttcacggac ttttataggg
    17641 tagcgcaaga aatagtcgcg taaaggattg agttgccgat tttccacaat atcaatcagt
    17701 tgatgggtct tgccgtccat catgacaaag ctcatggctc ccgagacatt tttcactgat
    17761 ttgaattcat cgaaacacac cacttctggt aacagctggc tcaaagaaat ggttttaggc
    17821 cgaaggctgc gaagtattcg aatgacagtg gtcggagaaa tgtgcttcat acgtgcgatc
    17881 aaagacatag aaagaggttc gcttaatagc tcaagaatgg cttgtttgac tctgcgagca
    17941 atggagcagc gacgatcact aactgaatct tctgctaaaa aagtctttcc acagtgttta
    18001 catttgaagc gctgtttgtt aatccgcaga atggtcttgt agttactgac atcattcaat
    18061 aagatggata cttttttcca gccctattta atgatcgttt gttcattttg atagtggcga
    18121 tgaaagcatt cctccggttg ataggtcaat gtgccatcaa aaacaaaaca tgtttcgtca
    18181 ttgattcgct ctttgcgaaa ggcatcttca tgaaagataa gatttaggtc tagaatatct
    18241 agtaactctt tggtaaactc tgaataggac atcttgaaac actcctttga atggttttcg
    18301 tcgactttat tctaaaggat tttaaggtgt ctgtttgcgt ttattagaaa aaagaaaaag
    18361 tattggcaac agaaggcgtc accaacacta gatattatag atcccttatt ttcttcgctt
    18421 caaattgtca aggagtcatc ataaattttg tatggattct tttgtttggg tagtagtctt
    18481 ctaaataatc ttggattaac accttcatct ctattttact tttatatttg ttggagtcga
    18541 ttaattctca tttgattgat cgatgaaacg cttcaattac tgcattgtca acaggagtcc
    18601 cagtttggct cactgaatgt gtgatgtggt ggtcgttcaa cagctcttga taaataaaag
    18661 accgatgctg gctacctcta tcggaatgaa ttaccgtagg caattctcct taataaattt
    18721 gtaatggacg gacgacaatc tcttttttca tgtgtgaatc taccatatag taacgtagaa
    18781 tacgagttgc taagtcaata taggtggaca aatagaggcg accgtcatta catggaatgt
    18841 aagtaatatc catcacccaa acagcatcaa tttggttctg ttcaaattat cgattcaaca
    18901 tttttttacc atttctttga ttgttttttc tctaccatga gaagatttgc gtcgatgaaa
    18961 tcctcttgtg cataaattca tttcacgcat aagaacagct acaacacgtt tattgatttc
    19021 gattccttca tcgtaaagtt tttgagtgat ccctggagat ccgtatcgat ctttgtgttc
    19081 gtagaacact cgtcttcctc ttttagtcgt ttattttcct gttcaagaat gtagaaatct
    19141 gctttatcag gtaatctttt tccttgtcca gcaaaagcat ttttcccgta tcgacgatat
    19201 tctgaaaccc atcgggaaat tgttggacga gagatattgt attcttcagc aattttcttg
    19261 gtagttactt ttttctcaag gatatcctgt gaaatcttta gtttaaattt acgatcatat
    19321 gtttttctca atagagttca ttccttccgg taacgtaact gatagtcaag aaaagtgtat
    19381 catagcccaa cttagtatca gggtttgtca actaaactgt ggaagttgat agataagagt
    19441 ttttaacaac caaaattctc ttgactattt ttgaaatcag acaaattttg attggacaca
    19501 tagttgaata aatctaccgt cgttacggaa ggtaaatcgc atgcttttca attctagagg
    19561 agaagtcctc gttgaagtac catagcccag ttctggattc gacctcctgc ccacttcata
    19621 cgtaaataaa gtaattttag gagtgagact gtgacaaaag ttatttttaa gctaaaaacg
    19681 aattaaaaaa cgttgataat taaaatcatt cgttttagat tctatgaatc attatagaac
    19741 gctgtaaaat cgatacttta taattataaa gaagatagtt tcaaatttat tgttcgaaat
    19801 ttgaaactat ctttttactt atgtcacaga ctcccttagt tgagttattc tttaatttcg
    19861 aatttcgaga tagctagata tattgcaagc atacattctt gataacaatc ttgatcatat
    19921 tttccagttc tccaggattg cttatgaatc aaagagctaa atcttttgag aattgcctca
    19981 acagcttctt catttccagc ctttgctaaa atgaaatctt cttttaaaga attcataaat
    20041 ctaccttctt cccatggact tataatctat ttctttggga aggaggagaa cgagcaaaga
    20101 agcatacaaa attagttatt atgcagatat taaaccagca ttttttgaat ttttttgtaa
    20161 agtcgatgtt ttaaatttgt cgcaccctgt cttgtaatcc ccagaacctt tccaatttct
    20221 ttatcagttt tatcaaagat aaatttttcg ataagaaaaa tttgttcagg ttctttcaac
    20281 aaaggaagga tttttgctaa ttttatatct tcaacaagga gagtcacatt taaaacatta
    20341 atcggttgtt taaattttat agtatcttta gtaaacaaat atggtgggag ttgatcaagt
    20401 tgttcatgtt tttgaatttc ttcttgtttt ttataataag tattagcggt gttcaaaatg
    20461 accttttcaa agaatcgaat caactgggca tttacgcgtt cgtcgttatc tgacatattg
    20521 cacacctcct ccattaagat ttaaagggaa aagaggcaaa aaacacgaaa agaagctaat
    20581 gcaattatgc tgcgctgcga ataacttcta taagctcaca tgcattgata ggactgctga
    20641 tgtttatttt caaaaatgtt tttttagttg ataatggata gttatcttta tacttagaaa
    20701 agaaaattca tatatcttct taaatatcaa acatgtcctt atggctaaga aaatgtcttg
    20761 agctttttac gtataataaa tcttttctaa aacctccttt tggataatat tcttattcgt
    20821 gtatagattg tattttttat ttcttccccc ttcataatta acaactagaa aagagttcga
    20881 atagacacgt atttgaacga aaatttttat tggattccca aattggaata tttagaaacg
    20941 aaaaattcct attatctgta tagtgcttag ataataacgg atcataataa ttattgtctt
    21001 tgaactagat atatgtaaat agtattcatc atgatgaaaa atgatgagtt ctcggtgata
    21061 aaatgtactt tttacggtgg aatatacatg aaagcgataa gtggatattt tataatgaag
    21121 taagttagct tattttgaca gaggaatagt attttagata acgtataaaa tactatttaa
    21181 tgatgtagaa aggaaagaga gaaacaaaag tgttttcaga taaagaagtc ttattaaagt
    21241 ctcttcatgt ttgtttaaag ttacctatcg tggtttttaa ttccgaattc agtattataa
    21301 aagagtatcg ctcagataga actacttatt ttttttatga ttataaaaag ttactaactg
    21361 ataatattaa aaaaactaaa gatttccatt tctttactgg tgattttaat gagatgtttc
    21421 ttctatacat gtttaaaacc agatattact tatttggtcc atttagagca aataatattg
    21481 ataaagtttt ttttaaacta aaaatgaaca atttgaatgt tgcaatggct gatagagaac
    21541 gactctataa ttcattgcaa aatttgactt tgtattcttt aggagacatt cgagatattc
    21601 tgattttagt tcattatttt tttactggaa aaatagaaga tctattccat gaaccgttaa
    21661 ttgaatatac agaaaatcta agtaaaacta tcgagcaaat caaaatagat aatctgttat
    21721 cacaaaatta cgatccagaa atatatctgt ttctttatga aaataaaata ctggaatatg
    21781 ttaaaaatgg agatattcga aatttagaaa acatggtttt taatctgagt aatggaataa
    21841 ttccaagtgt tagtggagat acgattcggt ctgaaaaaaa ttattctatc attgtttttg
    21901 aaaagttagc acaaacgtct ataacattag gaatggatat aatagaagcg tatcaaagta
    21961 gagatgcttt gatacaagaa aacgaattag ctgtatcgtt acctgaggtc ttaaaagtga
    22021 gggattctgg gattgtttat tatactaagg aaatcggaaa aactaagata gagcatcttt
    22081 ctcccttaat ttcttctgtc gttcaattca tcggcttaaa tatctataaa agaattactg
    22141 taaaggaaat agctaattat ttttctgtaa gtgaaactaa attaagagaa agctttaaga
    22201 atgaaatgca tattacgatt tataattata tctctaagcg aaaaatttct acggctaaaa
    22261 taatgttgaa atcaaaccat actatcagtg aagtttcttt aggtttagga ttttcagatt
    22321 cttcacactt ttcgagagta ttcaaaaagt acgcaggtgt atctcctaag caatatcaat
    22381 taggtttagt ggacaatttc aacaacatta gctgagaggt tttattttat tatgagacta
    22441 tatcatttac gtagtttgga ggagttgagg aaattggatt ttaacaaaaa atttttttgg
    22501 gaaatttctg atttgagttg tttcgtagaa tctgagcgtc taagtaatgg taataatagt
    22561 gtgttaaatg aatatgacaa agtagtaatt aataaagtaa aaagtttatg tgaagaagct
    22621 agagaatcta tcagtcatat taatggtagc aattttaaac aaacattatt taatctgatt
    22681 aagctagatg ctaaaatatc aagttattta ttctttttga tacatgatga atttatggat
    22741 tatcaaaaat tggatcagtt aattgaaaga gaaagctggg aagattatta cgtgggtcta
    22801 actttttcag aaaagttaaa caactacaag ttaatagatt ataagtattt gagtgaaagc
    22861 aattaattat tcatgtagag tactagacac tttaaatatg gacaattcaa ttataaagaa
    22921 aaagaatcaa taatttaatg aggctgtgac aaatgtcatt ttttgtttcg ctaaaaacga
    22981 gtgaaaaaat gttgataact ccaatcattc gtttaagatt ctaggaatca ttcagaaatg
    23041 ctgtgaaacc gatactttat aattataaag aaggtgattt caaattccga agaaggaatt
    23101 tgaagtcacc tttttactta tgtcacagtc tcattcagta atgcgggata aggctcctta
    23161 ttccgttatt tgaatactct gaagtttaat ctaagaaagg gtaatatttt gaaaacatgc
    23221 ttcatcgcat agaaatgaac ttaatttcag aaagaataca tacatatctt tttatatgtt
    23281 ttcagctgct gaatggttaa aaagcacaaa tatccgaaaa aaacatgaag aaatgtactt
    23341 tttttgttgt aatgttcatg aaattctcta agtaaattag ttaacataag gttagtaaga
    23401 ttcctattct gtgaataata cgtatcagat ttatctttta ttcttactag gaaagtaatt
    23461 attaagggag ttgatttgaa taatggttag caagaataat aagagagtat ttctagaaaa
    23521 aacaaagaaa cgagtattga agtacagtat caaaaaatta agtgttgggg ttgcatctgt
    23581 tttgattggt gttggtcttg tcttgggaac aactgaacta gttaaagcac aagatgaaat
    23641 ttctccaagc agcccacttg aaacagcgat aagctccgta caagtaggtg acaaagtagc
    23701 ttcggggaat acttttcagg agaatcctgg ttatacgaaa aattataact tttcggattt
    23761 acaatttagt cctcaagaac taactggaga cacactaaaa ggcaatacaa ttggatttga
    23821 agtttacgga aaacataata ttgcagcatc aactaaaaac tgggaaatcc gtcttcaatt
    23881 agatgaacga ttagctaaat atgtagaaaa aattcaggtc gatccaaaga aaggaattgg
    23941 atcttctaga cggacctttg taagaattaa tgattcgctg ggtagaccta caaatatttg
    24001 gaaggtcaat tatattcgtg caagtgatgg attatttgct ggggcagaaa caaccgatac
    24061 gcaaaccgct cctaatgggg taattacgtt tgaaaagagc ctagatgaaa tttttaaaga
    24121 gataggcata gacaatctca agaccgatcg tttaatgtat cgaatctact tggtaagtca
    24181 tcaagacgat gataaaattg taccgggtat tgatagtacg ggctactttt taacagattc
    24241 agatgatttc tataatagct tagatgtatc tgaaaataat cctgaccagt ttaaacatgg
    24301 atcagtaagt gcaaaatatg agaaacctaa tactcaaaca aaagatggtt caggatccac
    24361 aggtgctaat ggagctataa tcttagatca taagttaacg aaaaattata atttctcata
    24421 ttcatcttcc gcaaagggca cgccttggta tgctaattac aaaattgatg aacgattagt
    24481 tccatatgta gcgggtatac agatgcacat ggttcaggct gacaaagtaa catatgatgt
    24541 ttcgtttgaa tccggaacaa aagtagctga tttagcaatc gagcgacggg aaggtcatga
    24601 aaattatggc ataggttcaa taactaataa tgatttaact aaacttatcg attttgctaa
    24661 tgcaagtcca cgtccagtcg ttattcgata tgttttacaa ttgactaagc cattagatga
    24721 aatcttagaa gatatgaaag caacagcaca agttgaagaa aataaaccat ttggtgaaga
    24781 tttcatcttt gattcttggt tgtcggatac gaataaaaaa ttaatccaga acacttatgg
    24841 aacaggttat tattatttgc aagatattga tggtgatgga aaccctgacg ataaagaaga
    24901 gagcggagac acgaatccat atatcgggaa acctgaatta gaagaagtat atgatgttga
    24961 cacaacagtt aaggggaaag tattcatcca cgagttagcg ggaacaggtc acaaagccca
    25021 acttgttgat aaagaaggta ctgtattagc agaaaaaact atcgctccaa atgaaaaaga
    25081 tggggctcca atttcagata ctgtagaatt tgaatttacg ggtgtagatt caagtaaatt
    25141 aatcgcgaaa gatgaattaa aaatccaaat cgtttctcca ggttttgata aaccagaaga
    25201 aggttcaacc gttattaagg aatcaccaaa agcggttgat aaacaaaccg tggtagttgg
    25261 atttaaacca gatgctaaag aatcaattcg gaataataaa aacttacctg aagatgcaga
    25321 gtattcatgg aaaacagagc ctgatacttc taacgttact gatagtacga aaggtattgt
    25381 aactgttaag atcggaaatc gaactttcga cgtggatgta gagtttgctg taaaagcttc
    25441 tcaagctatg gaaaatgatg caacatacgt acctataaca acaactccag aaacgacagt
    25501 tcaaagtgga aaacctacat ttgataaacc agatgttcct ttagctaaag atgccttttc
    25561 aatttcagat gtctataata aggacttcgg taatgcaagt gttgatgcaa acactggtat
    25621 tgttacattc actccagcta aaggtgtagg agaatcggag ccgattactg gaacaattcc
    25681 tattaaaatt gtttaccaag atggttctgt aggcacgacc gatttagcag taactgtaag
    25741 taaagatatt tatgaaaatc caggagaaaa cattcctgca ggctaccaca aagtaacctt
    25801 caccgcaggg gaaggaacaa gtattgaaag tggaacaaca gtctttgcag tgaaagacgg
    25861 cgtaagcttg ccagaagata aacttccagt gttgaaagca aaagatggtt atacagatgc
    25921 gaaatggcca gaagaagcaa ctcaaccaat tacagcagat gatacagaat ttgtatcaag
    25981 tgcaacaaaa ctggatgata tcattgaaaa tccaggagaa aacattcctg caggctacca
    26041 caaagtaacc ttcaccgcag gagaaggaac aagtattgaa agtggaacaa cagtctttgc
    26101 agtgaaagac ggcgtaagct taccagaaga taaacttcca gtgttgaaag caaaagatgg
    26161 ttatacagat gcgaaatggc cagaggaagc aactcaacca attacagcag atgatacaga
    26221 atttgtatca agtgcaacaa aactggatga taaatctgac gctgacaaat ataatcctga
    26281 aggtcaaaaa gtgactacag aattgaataa ggaaccagac gcatttgagg gaattaagaa
    26341 caaagaagat ttaccaaaag atactaagta tacttggaaa gaaaaagtag atgttagcgc
    26401 agctggaaat aaaaaaggga ctgttgtagt gacatatcca gatggatcat ctgatgaagt
    26461 tgaagtagat gttacagtga cagacaatcg ttctgacgcc gataaatatg agccaacagt
    26521 agaagg
//
LOCUS       DACKAC010000037        25996 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.037, whole genome shotgun sequence.
ACCESSION   DACKAC010000037 DACKAC010000000
VERSION     DACKAC010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25996
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.037"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            289..606
                     /locus_tag="I0B46_001600"
     CDS             289..606
                     /locus_tag="I0B46_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083906.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNASIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(697..1062)
                     /locus_tag="I0B46_001601"
     CDS             complement(697..1062)
                     /locus_tag="I0B46_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083907.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1270..1752
                     /locus_tag="I0B46_001602"
     CDS             1270..1752
                     /locus_tag="I0B46_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083908.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     LVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1949..2839
                     /gene="pstS"
                     /locus_tag="I0B46_001603"
     CDS             1949..2839
                     /gene="pstS"
                     /locus_tag="I0B46_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HAR2083909.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            3165..5639
                     /locus_tag="I0B46_001604"
     CDS             3165..5639
                     /locus_tag="I0B46_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAR2083910.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLLKRRNNTVAMTGDGVNDI
                     LAMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLV
                     KNIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNV
                     MEKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKF
                     RSQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLF
                     ERKNEKEQKPFSRVVKKFLSIFKREN"
     gene            complement(5742..7478)
                     /locus_tag="I0B46_001605"
     CDS             complement(5742..7478)
                     /locus_tag="I0B46_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAR2083911.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNGDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRAYTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLHGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(7475..8179)
                     /locus_tag="I0B46_001606"
     CDS             complement(7475..8179)
                     /locus_tag="I0B46_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAR2083912.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            8310..8813
                     /locus_tag="I0B46_001607"
     CDS             8310..8813
                     /locus_tag="I0B46_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAR2083913.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            8773..9111
                     /locus_tag="I0B46_001608"
     CDS             8773..9111
                     /locus_tag="I0B46_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAR2083914.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            9132..10550
                     /gene="ffh"
                     /locus_tag="I0B46_001609"
     CDS             9132..10550
                     /gene="ffh"
                     /locus_tag="I0B46_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAR2083915.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            10662..11240
                     /locus_tag="I0B46_001610"
     CDS             10662..11240
                     /locus_tag="I0B46_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAR2083916.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            11244..11765
                     /locus_tag="I0B46_001611"
     CDS             11244..11765
                     /locus_tag="I0B46_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAR2083917.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(11844..12398)
                     /locus_tag="I0B46_001612"
     CDS             complement(11844..12398)
                     /locus_tag="I0B46_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAR2083918.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            12651..12926
                     /gene="rpsP"
                     /locus_tag="I0B46_001613"
     CDS             12651..12926
                     /gene="rpsP"
                     /locus_tag="I0B46_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAR2083919.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            12938..13189
                     /locus_tag="I0B46_001614"
     CDS             12938..13189
                     /locus_tag="I0B46_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAR2083920.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            13313..14104
                     /locus_tag="I0B46_001615"
     CDS             13313..14104
                     /locus_tag="I0B46_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083921.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTATTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            14274..14795
                     /gene="rimM"
                     /locus_tag="I0B46_001616"
     CDS             14274..14795
                     /gene="rimM"
                     /locus_tag="I0B46_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAR2083922.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            14785..15546
                     /gene="trmD"
                     /locus_tag="I0B46_001617"
     CDS             14785..15546
                     /gene="trmD"
                     /locus_tag="I0B46_001617"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAR2083923.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            15682..16029
                     /gene="rplS"
                     /locus_tag="I0B46_001618"
     CDS             15682..16029
                     /gene="rplS"
                     /locus_tag="I0B46_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAR2083924.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            16339..16581
                     /locus_tag="I0B46_001619"
     CDS             16339..16581
                     /locus_tag="I0B46_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAR2083925.1"
                     /translation="MNDGKVKQVPSSTKKKNILLKEVLKRFDHGVTYTETEVNSILLN
                     VFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            16932..17186
                     /locus_tag="I0B46_001620"
     CDS             16932..17186
                     /locus_tag="I0B46_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAR2083926.1"
                     /translation="MLELSKQLPVSDPRHFDYEEIAIKILEELQKNYTTKRVNGSNGL
                     LLHAVYDKNSLKGVDECVIWGDYFYVEGITRLAKTWYCYW"
     gene            17215..18474
                     /locus_tag="I0B46_001621"
     CDS             17215..18474
                     /locus_tag="I0B46_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAR2083927.1"
                     /translation="MKRWKKMMIGSIVLGMSLVFGACGNQEDSSEESANEITVSTWNY
                     ETTPEFKALFEAFEKKTGIKVKAVDIASDDYDTKLTTMLSSGDTTDVLTMKNLLSYSN
                     YALRDQLVDQTERIRELDTDAAEETYEMYDIDGKTYALPYRTDFWVLYYNKKMFDDAG
                     IEYPKNLTWDEYEDLAKKLSKNDGQVYGAYQHIWRSTIQAIAAAQNEANLVEPDYQYM
                     TDYYDRALRMQKEGAQMDFGTAKSTKVTYQSQFEEQKAAMMYMGTWYMAGILANKEAS
                     KTEVEWGITAIPQKKKGESVTTFGSPTAFAVNKNSKKQKAAQEFIEFAASEEGAKVLA
                     GVGVVPSYRTDEIDQLYFNLAGMPTDEISKKAFSPDEIKLEFPIDTYGPAIDKILQEE
                     YDLVLVGDETPEKGTVNMEKRVAAEKE"
     gene            18527..19423
                     /locus_tag="I0B46_001622"
     CDS             18527..19423
                     /locus_tag="I0B46_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAR2083928.1"
                     /translation="MENSINKKATNHLRRKRTWIAYSFIAPNFIGFFVFTLIPILFSL
                     VLAFMKWDSFSTPEFAGLDNFRKMANDETFWISLKNTFLYTAAVVPGTLVCSLGLALL
                     LNQKIRGMKLFRTIFFFPYVTSLVAIAVVWNMLFHPTVGPINIFLRRFIEHPPGWTSS
                     SNWALWAIVIVSIWRGMGYYMILYLAGLQSIQKELYEASSLDGANRWKQFLNVTLPSL
                     RPTTFFVTIMLVINCFKVFDLVQVMTDGGPGRATNVLVYQIYNEAFKKFDFGYASAIS
                     LVLFVIVLGVTVVQFSWNKRRT"
     gene            19434..20303
                     /locus_tag="I0B46_001623"
     CDS             19434..20303
                     /locus_tag="I0B46_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAR2083929.1"
                     /translation="MEESVRNERKKAKIRSKNMPFIQWLGMILLIVLALLTIIPFLWM
                     VSASFKANNEVLTIPIQWIPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFT
                     SSFAVYGFSELNFKGRDILFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQ
                     AFNAFGVFLLKQYYSSIPDSLCESARIDGLSEWEIYRKIILPLTKPAIASLTIITFVN
                     TWNDYMGPFIYLSSTENKTIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQK
                     YFVEGIASSGMKE"
     gene            20313..20988
                     /locus_tag="I0B46_001624"
                     /pseudo
     CDS             20313..20988
                     /locus_tag="I0B46_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
     gene            21194..22858
                     /gene="alsS"
                     /locus_tag="I0B46_001625"
     CDS             21194..22858
                     /gene="alsS"
                     /locus_tag="I0B46_001625"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAR2083930.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            22871..23581
                     /gene="budA"
                     /locus_tag="I0B46_001626"
     CDS             22871..23581
                     /gene="budA"
                     /locus_tag="I0B46_001626"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAR2083931.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            23711..24208
                     /locus_tag="I0B46_001627"
     CDS             23711..24208
                     /locus_tag="I0B46_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAR2083932.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            24393..24653
                     /locus_tag="I0B46_001628"
     CDS             24393..24653
                     /locus_tag="I0B46_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083933.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVIGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(25015..25254)
                     /locus_tag="I0B46_001629"
     CDS             complement(25015..25254)
                     /locus_tag="I0B46_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083934.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            25540..25854
                     /locus_tag="I0B46_001630"
     CDS             25540..25854
                     /locus_tag="I0B46_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083935.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT         8634 a         4373 c         5306 g         7683 t
ORIGIN      
        1 agattacaaa tagtatcgta tttcgcaatc ttaaaattat cttcttactg tgaataagat
       61 aataaaattt gtatttgtcc tttttaggta acttatttta atctatatac ctttctggat
      121 caacgaaagt atactttata tagtcataac gccgatggta actatgcact gtaacgataa
      181 tgctgctaaa atatcttttt acgaaaaaca tcacaaggaa aaacttaact atggtattat
      241 ctaaacataa ttttttcggt atttaatatt taattaggag gtaatatttt gcttttttat
      301 agtaatttta tcttaatagt agcgatatta ttacttctga atatatggat ttttgatagg
      361 agtagaaatg caagtatagg ttttagaact aaacgaagtc tgtcttctaa aaaaaattgg
      421 gtatactcac aaactatttt ttatggagga attgttttaa ttagtttact ttcttcaaca
      481 ctttattctt tgaatattat agatgtttcc acctcaaatt caatatctat tattggaatc
      541 attattgctg caattattac acaactgttt ttagtgtttg gagagaagaa aagaagtaaa
      601 aaatagaaaa actagtgata gttttggtga tataagaaaa acatatatgt ataaaaagaa
      661 gaaagtagct tctctttgta catatatggt ttgtttttat ggatgccgag cttttttata
      721 agataaggct accgcccata acgacccaag gatttgtgga actaagtaaa tacctgcaag
      781 aagcaagatc agctgggtcg gttttaaagg atgatagaag taacgccatt cagtaaaatt
      841 ggcgcaaaat aaataggaag tgacagtaga tataatattg gcaatgaata aagatataat
      901 acgcctgttc ctttgaaaat aatatccagc aaaaagagat aaaaatatca atgtgatgaa
      961 cccgcttacg gaatgataac gaaaatcaag cagtaaggcg aaatgcacat aaggaacaaa
     1021 agataaaagc aacggaaggt ttttccagca agtttttatc atctttcatc accacctaac
     1081 aagtgttatt attttgttag ttttattgta aattgaatcc tgcaataatc aattactttt
     1141 tcattatttc tagattaaat gaataaactc tcatgccatt atctctaaaa aaaaagtttt
     1201 tccccctaat ttttttagat agaaaattgt atagtatgat gagagaaaaa gtagtttggg
     1261 ggaacgttaa tgagaagaaa agcagtaatc gatcggcttt atttattttt ttggtttttt
     1321 cctatcgttt tgctaccttt tacatgggta aatgctgttg tacaaggtgc ggagatatta
     1381 ggcctgatcc aaggaagcgg attagtggtt tctaggaatt ttccagcttt gactgttttc
     1441 tatttagtga taagtgtagg tctcttatta ccaaaatctc tgatcaatca ctacaattgg
     1501 cagctgcttt acttttcttg cttattagta tttacatcta tgttttcttt gctgcctcgc
     1561 ttgattatcg gccctaatgg tttgagcgag agtggattga aggattcagc ttattcctcc
     1621 atgtatttct tgacgatcaa accatggttc tatttagtaa ttgcctcatt tattcttgca
     1681 acattttttt acttctggac agaaatcagg ataaaaagtg aacgatctgt atctataaaa
     1741 aataactatt gatggtggat catcagtaaa aaagcatatg agctgcgtaa aatgcatgtt
     1801 tctcatatgc tttttttatt attatgtaaa gaatgcgaaa aacgtttaca tgatttttac
     1861 ataacttaac tatattttta cagcaaccat acgagggatt tacagtcgac tgataaactt
     1921 tagttgtaaa taagaaagga gcaataaaat gaaaaaagta ttgtttactc taggtatgat
     1981 gggaattctt ttagccggat gcggctcagg atcaggaaat acagatggct ctgccacaca
     2041 aaataattca agtgactcaa atgaacaagt aaaaatcgtt gcggtaggtt cgacagcatt
     2101 acagccgtta gtcgatgcag cacaagaaag ctttgtacaa gaaaatccaa attatcaaat
     2161 ctctgtacaa ggaggaggaa gcggaactgg tttgagccaa gtcgaagctg gagcagttac
     2221 tatcggaaac tctgatgtat ttgcagaaga aagagacgga gttgatgctt caaaattagt
     2281 ggatcataaa gtagcagtgg tcgggatggc accaatcgtc aataaagata cagatgtcaa
     2341 agatatcacc aaacaggaat tgattgatat ctttacagga aaaatcacta actggaagga
     2401 agttggcggg aaagatcaaa aaatcaacgt cgtgaaccgt gcaaacggca gcggtacccg
     2461 cgcgacattc gaaaaatggg gactggatgg tgctacccct gtgcagtccc aagaacaaga
     2521 ttcatcagga acagttcggc agctggtaag tcaaacacca ggagcaatca gctatttagc
     2581 tttctcatat ctcgatgatt ccacacaagc actaagtatc gacggtgtag aaccaaaaga
     2641 agagaatgta gcagataaca gctggggaat ctggtcatat gaacatatgt acacgaacgg
     2701 aaaaccttcc cctgaagtcc aaaaattctt agactacatg atgacagaag aaatccaaga
     2761 aggacctgtc aaagaattgg gctatttacc aatcacaatg atggaagtag aacgtgatca
     2821 cgaaggaaat atcaaataac tagcagataa acatgccacg gtagtcgtta ctattaagga
     2881 aaaatcaact atgtttcccc acatatacat ttttccttta attgtgaaaa atagttcctc
     2941 atattttgcc gttttttgag ctaataccga gggtcagctg attgaacacc gtttattcga
     3001 aaaaaagagc tacgacatag aactgtcgta gctttttttt tgctatatga caggaatgaa
     3061 aggtgtttgc aagcttaatg tgaggaacac tttccatatc tgttctatac tggataggag
     3121 aagtttggaa atatccagaa aacgaaatca atcgaggtag ccacatgaag aataaatgga
     3181 taaatcgaag taaaaaaaag aaagtaacac aaagatcatc agataaagtc atccgtcttt
     3241 ttccatcgta taaagaagga ttaacggcag atcaagtcaa ggaacgaata gaaaaaggtg
     3301 cagcaaataa ttcagtcgat cccacattca aaacaaatca gcaaatcgta ttggaaaaca
     3361 tatttacgta tttcaatttg atctttctta tcttagccat tttgctttgt ttagtagaat
     3421 cctataagaa cttgactttt ttaccagtta tcatagccaa tacaggaatc gccatttttc
     3481 aggaaattcg atccaaaaag atcttggacg aactaaatgt tttaaataca gcaaaagtca
     3541 atgtcatacg agacgggata gaacaaacaa tagatataga agaccttgta atagatgaca
     3601 ttgttatttt agagactgga catcaaattc cagcagatgc agaggtcgtg gaagggaaac
     3661 tgcaagttaa tgaggcttta ttgacaggcg aagcagatga aataaccaaa gaaataggcg
     3721 atccattaat gtccggcagt ttcattgttt ccggaaaagc ttacgcccga ttagacaaag
     3781 ttggagcaga ttcgtatatt tcccagctga cagtcaaagc caagacaatg ggagaaggag
     3841 aacagtcgga gatgattgct tctcttaatc aattgatcaa atggattggg atcatcatta
     3901 ttcctatcgg tatcctgctt ttttctcaaa gttacttttt taatggaaac acgatcaaag
     3961 aaagtgtcgt tgcaatggaa gcagcattga tcggtatgat acctgaagga ctgtacttgc
     4021 tgacgacgat tgcattagca ttaagtgcga ctaaactagc aaagcaaaga gttcttttac
     4081 acaatatgaa aagcattgag acattggcaa gggtaaatgt cctttgtgta gataaaacag
     4141 gaacgatcac agaaaataaa atgtctgttc aaaaagtgat cgtctctaaa aaacaagaaa
     4201 taatcagtca gtcagatcaa ttggaagcat gcattgctga ctatgcaaaa gcgatggcag
     4261 gagacaatgc aacaatggaa gctgttaaag catattttac aaaaaccact gatcaatcct
     4321 atcaccatgt tattcctttt tcttctgtcc aaaaattcag cagtgtagcg ttggcagaca
     4381 aggtgtatgt tttaggagcg cccgaaatgg ttttgcgcga tcaattgccg gaatatgcgg
     4441 aagagtttgt ttctttcgct gaaaaaggct atcgcgtgtt ggtctttggt gtgtacgatg
     4501 ggattcttga agaaccagta ttgacagaag cagtcacacc attaggctat attctgattg
     4561 ctaatcctat cagaaaagaa gcaagagcca catttgatta cttcaaaaaa cagcaagtag
     4621 ccatcaaagt gatttctgga gataatccgc tcaccgtatc caatgtagct gtccaagcag
     4681 ggatttcaca tgctgatcag tacgttgatg catctaaatt atcagaagaa gaatatgaag
     4741 cagctgtcga aaaatataca gtttttggac gcgtcaagcc agaacaaaag aaaatcttcg
     4801 ttcaattgct aaagcgtcgt aacaatacag tagctatgac aggtgatggc gtgaatgata
     4861 ttctagccat gaaagaagca gactgcagta tagcgatggc ttccggaaat gaagccgcca
     4921 tgcaggcctc acaagtggtc ttacttgatt ctgacttttc acgcatgcca gaagttgttg
     4981 cagaaggacg gcgcgttgtg aacaatattg agcgatcagc cagcttgttt ttagtgaaga
     5041 atattttctc ctttttgctc tcactgtttt cagtgatctt tgctctgacc tatcctttag
     5101 aaccatcaca aatcacattg attagtttgt ttactatcgg acttccatct ttcttgcttg
     5161 ctctagaaga aaacaaaaag cgaatcagag ggaaattcat catgaatgtg atggaaaaag
     5221 ctgtgccagg tggactaaca gatatgatcg tagtcggtgc gttggtgatc tgcggggtga
     5281 ctttgaatct aaataaaaca gatgtttcta cagcatctac catgcttttg attgcagtag
     5341 ggttcctcgt tctctataaa atctgttcac cgctgaataa attcagaagt cagatcattc
     5401 ttttctgtgc tagcggtaac tttttttcgg tcatcttttt gcataagctg ttttcaatca
     5461 ccggtatctc agctgtatca cttttgctgt tgagcattct atttttctcg gcagattcga
     5521 tttttaggca tttgaccaca ttggtagaat atttgtttga acgtaaaaat gaaaaagagc
     5581 aaaagccgtt tagtcgcgta gttaaaaaat ttctatcgat atttaaacga gaaaattgac
     5641 gaaaagtatc tcaaaaaaga gtaaaaaaga acaaccactc gtgtatcttt cgaagcagga
     5701 tgagtcatcc aactttaaaa cgcacgagca gttgctcttt tttacatttt cggaatggta
     5761 acggtgaaag tcgaacctgt tccaagatga ctgtctacag taatcgtacc atgaaggagt
     5821 tgcacgtagt cttttacaat cgctaaccct aatccggtcc cgcctgaatg ccggcttcgc
     5881 gccttgtcta cgcggtaaaa acgctcaaag atccgttcct gatcctcaat gtcgatacct
     5941 ataccaaagt cttggacaga aaacgataga tcatctgttg cctgatagcg gataataatc
     6001 tcgctgtcgc tttttgaata ttggacagca ttttcaatca gatttttgat aattggataa
     6061 aggatatctg ttttagtagt aaatggttcg ttctttccaa tcaatcgaat cgtcaaccgt
     6121 ttcttttcga tgattggctg ataattttga acgatttgct ggaaataagg ttctgtataa
     6181 attgtctgga tctcgtaaga attctcgcca tcttttgaca gctggatgat ttcccgaatc
     6241 agtttatcta accggatcgc atctttttgc atgatctcaa gaaaagaagt cagagtttgc
     6301 gggtcatctt ttgcaccatc cagtaaagtc tcggtaaaac caatcagtga agtcacagga
     6361 gtttttaatt cgtgagagac gttgccaacg aaatcctttt gtagcttttc taaacgtcgc
     6421 actttcgtta agtcatacgc cacccctaga atctgtccgg taccttctga attagtaaag
     6481 tagcgcaaac tcaagtctaa tgtctgccga ccttctgtaa gggtgatctc ctgatggatc
     6541 agcggatgct caggagtcac ttgatgaatc agctgaatga gttttggctc ttgaatgact
     6601 tctgtgtacc gctgttcagg ttgatagctt accacgttta aatgtagctg catctttgga
     6661 ttaagcagta gtaaacgaga atcagcatca tcaatcagga atacgccaat catcagttcg
     6721 ttcagtaacg tatataattg ctcttctgtg gtagtatacg cccgatacat cttactcatt
     6781 tgttcgctca acgcattgat cgtctgatac aattcttccc attgatcggg tgtttgcatg
     6841 attacttccg cctgattagg attttgcacc atttttttta gtacgggcag cacggttttc
     6901 aatggctggt ttttttgata gatcagataa tagatcattc ctgcaagaac aaggaagaaa
     6961 gacagaaaga agaaaaagac ccaacggcga aaactctcag ttctcggtaa aaaaccgctt
     7021 gtcggttctg cgatacgtaa aatggcttct aactggccac tttttttgac tggtaatgcg
     7081 acatataaaa gatccccatt caaagtagta cttttgcgca aagcgctacc taggttccca
     7141 ccattcaata cggctttgat ttcgggacgg gagtttcgct gttcattcaa tgtttcattg
     7201 tttgtgtcaa ataagatctt tccagtagca tccattaaag taatccgttc gttcgactga
     7261 tggacgaatt cttccaaagc agttttgttt tgcgctgctt ggaggttttc gacatctaat
     7321 tgccggatca gcagttgtgc tttttttgtc aggtagcttt cttgctgttc cacgatctgc
     7381 tgttgaaaat aatgggaaat caattgccag ccaccaaaaa acaaactaac taaaagaata
     7441 agaaagaaga gacgttcata aatttttttc catttcatcg tttgggttcc tgaaacttat
     7501 agccaaatcc tctaatagtc accaagtact ttgggtgttt tggatctcgc tcgatttttt
     7561 ctcgcaaatg actaacgtgt acatctacga ttcggctttg tcctgcgaag tcaaaattcc
     7621 aaatgcggtc tagcaatgtg tctcggtcga tcacacggtc ttttcgtttc atgaaataaa
     7681 caagtaattc aaattctttc ggtgttagtt caatcagatg gtcgtctacg gtcacttgat
     7741 aattggtcaa atcagcagtg atttgtccaa tcgatagata ttcaggctca gcttcatctt
     7801 ttgattgctt acgaggttca attcgtctga atattgcttt catacgggct aatacttcgc
     7861 gaggactaaa tggtttggtc aaatagtcgt cagcacctat ttctaggcca atgatccgat
     7921 ccacttggtc atctttagcc gtcagcatca aaatcggtgt ttcatttttt tcttgacgca
     7981 gccgctgagt gatctccatg ccatccatcg ttggaagcat cacatctaaa atgatgaaat
     8041 caaatgattg ttctaatgct aattctaatc cttcgcctcc atctgtggca ctggttacct
     8101 tgtagccttc tttttccaaa ttgaacgtca agagcgttac aatagaaggt tcatcatcaa
     8161 caactagtat ttttttcatt caaatctcct ttaaataggt ttcattctgt ttatatttta
     8221 gcatagaatc agtacaaagg gctattttac aaaagaaata aatggtataa taggaaggca
     8281 ctttgctgaa tagaaaggaa gagcagtcaa tgattggaat cagtgcatgt ttaggcggtc
     8341 tggcctgtcg ctatgatgga cagaaaaaag aagtaccaga attgatggaa ctagttgaaa
     8401 ctggagaagc tgtcatggtc tgtccagaag tgatcggcgg attaccaatt cctagagatc
     8461 ccgctgaaat cattggtgga aatggattcg atgtgtggaa agatcaagca aaagtatgga
     8521 cgacaagcgg cgaagatgta acggaagcgt acaaaaatgg cgcgatcaaa gcctatcaaa
     8581 aattgcagga aaaacacatt gatctagtca tcatgaagga aaaaagccct tcatgcggaa
     8641 cccattcgat ttatgacggg agtttttccg gaataaaaaa aaatggtgcc ggagtggcta
     8701 ctgcttattt tattcagcaa aaaatgactg tgctctctga tgaagaatgg ctgaaaacta
     8761 gaggtgagca gaatggaaat cgaaaagacc aatcgaatga acgcgctctt tgaattttat
     8821 tcgaccttat tgacagaaaa acaaatgaac tacatggaat tatattatgc agatgatttt
     8881 tcattaggtg agatcgcgga agaatacgaa gtaagccgac aagcagtata tgacaatatc
     8941 aagcggacaa gcaagatctt ggaggattat gaaaaaaaac ttcatctttt ttctaattat
     9001 attgttcgag aacaggtatt ggaaaatatg actgcatata ttacagataa gtacccggaa
     9061 gataaaaaac tacttgaata tgtacagcaa attcaagcaa tcgaagaata aataaaagga
     9121 atgaagaaaa tatggcattt gaaagcttaa ctgaacgtct ccagcaggcg atgggaaaaa
     9181 taagaaaaaa aggaaaagta tcagaagctg acgtaaaaga aatgatgcgg gaaatccgcc
     9241 ttgccttact agaagccgat gttaacttgc aagtagtcaa agattttact aagcgcgtac
     9301 gcgaacgtgc ggtaggggca gaagtgctgg aaagtttatc acctgcacaa caaatcgtaa
     9361 aaatcgttga tgaagaattg acgatcacac ttggttctga aacagcagag ttaaataaat
     9421 caccaaaaat accaactgtt atcatgatgg ctggtcttca aggggcaggt aaaacgacct
     9481 ttactggtaa attagccaat tatttaaaga aaaatgaaaa tgctcgtccg ctcctaatcg
     9541 caggggacgt atatcgacca gctgcgatcg accaattgaa agtattaggt caacagctag
     9601 acgttcctgt atttgatatg ggaactgaag tcagcccggt agagatcgtc cgtcaaggga
     9661 tggagttagc aaaagaaaag aaaaacgact atgtattgat cgatacggca ggacgtctgc
     9721 atatcgatga aacattgatg gatgagttga aacaaatcaa agaattgacg caaccaaatg
     9781 agattttgct tgttgtcgat gccatgactg gtcaagatgc cgtcaacgta gctgatagct
     9841 tcaaccagca attaggtatt acaggggtcg tcatcactaa acttgatggg gatactcgag
     9901 gcggggcagc actatcgatc cgctcagtga caggcgcacc aatcaaattc atcggttctg
     9961 gtgaaaaatt gactgactta gaagtattcc acccagatcg tatggctagc cgtatccttg
    10021 gtatggggga tatgctgaca ttgattgaaa aagcgcagca agactatgac gagaaaaaag
    10081 cggaagaact tgcacagaaa atgcgggaaa attcattcga tttcaatgac tttattgaac
    10141 agcttgatca agtgatgggt atgggcccac tggaagatct aatcaaaatg atcccaggaa
    10201 tgaatcaagt ccctggaatc gaaaacgtca aggtagatcc aaaagatgtt gaacgcaaaa
    10261 aagcgatggt ttattctatg acgccagcag aaagagcaaa tcctgatctg ttgaacccaa
    10321 gccgtcgtcg ccgaattgct gccggttctg gaaacagtgt tgttgaagtg aatcgaatga
    10381 tcaaacaatt caaggaatca agaaaaatga tgcagcaaat gtccaaaggg gatatgaaca
    10441 tcccaggtat ggaccaaatg tttggttcag gcgtcaaagg aaaactaggc aaaatggcga
    10501 tgaatcgaat gatcaagaaa aataagaaga aaaagaaaaa acggaaataa gatgaaaaaa
    10561 acagcttcgg tacagactga aaatgtctac tgccggagct tttctatcag caatttttat
    10621 tctttactaa tacaattact ctaaacgaag aggtggcaca gatgagatgt ccgtggagtg
    10681 aacaaacaga aagtatgcgg caatatcatg atactgtctg gggtgttcct gaatatgatg
    10741 accaaaagct gttccgaaaa ttgatgcttg atattaatca agccggattg agctggcaga
    10801 cgattttaaa taaaagtgaa gcattcgatg aagcatatga tcgattcgat attaaaaaag
    10861 tcgcaaatta tggagaaaca aaaatcagtg aactaatgga aaacaaagga attattcgta
    10921 accgaagaaa aatcgaagca gccgtccaaa acgccaaagc agtcttgaaa attcaagaag
    10981 aaatcggcag tttttcagag tatctttggt cgttttctaa tggaaaaatc gttgattctt
    11041 cttatcattc acaagaagaa gtcccaacaa caagtcctct ttcagatgaa ttagcaaaag
    11101 atctaaagaa aagaggattt aaatttatcg gaagcacaac aatctatgct tttttagaag
    11161 ctgtcgggat catcaatgat caccttgaca gttgtttccg caaagaagag gtcgtacgta
    11221 tcggcagaaa gggcgaataa agcatgcgaa aaatcatatt ttatggtgct ataagtttag
    11281 atggctattt ggcaacgaaa acggatgatc ttcaatggct ttttgacact ccaacaggta
    11341 agcaaaccac ttatgaagct ttttatgaaa caatcgatac gaccatcatg ggcagaaaaa
    11401 cttatcagga agcaaaaaaa tatttggaga ccgaagcaat ttatccagag aaaaagaatt
    11461 tcgttttttc cacccacgca caattacaat tgccagatgc tgaggtcgta aacgaagatc
    11521 ctgttgcttt tctaaaaaat ctacaaatga cagaaggaaa agatatctgg gttgtgggtg
    11581 gcggtcgatt gatcaaaccg ctattggaac atcaaatgat tgatgaatgg tatatccaga
    11641 ttacccctgt attattaggg gacgggattc ctttattcca aaaaggaaca tatgaagcgc
    11701 gttttgaaat gctagacacg acacgattcg gtgaatttat cgaattgcat tatgtacgaa
    11761 agtagaagta aataaaataa aaaaggcaac tatatgcaga aatgtcctgt acatagttgc
    11821 cttttttatt accgttatgc tttttactct gttaatttgt tagaaaaatc tagatacgca
    11881 tctaaaatcg tttggaagaa acttaatgtt ccttcaacga tattaccatt ttcatcaatt
    11941 agattcgcaa cgtttccgat atatgcttcc ggttgctgca aggttggcac attcaaaaag
    12001 acaagtgatt gtcgtagatg gtggtttgcg ccaaagccgc cgatcccacc tggtgatgca
    12061 gtaacgacta aacctggttt atgatcccaa acactgtgtc cataaggtct tgagcctaca
    12121 tctaaagcat ttttcaatac tgctggcaca gaacggttgt attcaggagt aacgaaaacg
    12181 acaccgtcta gtcccctcac ttcctcacga aaacgagtcc attcagcagg agcctgttct
    12241 ggagtttcga gatcctcatt gtaaaatggc agatcatcga tttttacaaa tacactttcg
    12301 tacccttcag gaagcaattt cgcaaaagct tctgctactt ttttgttgta agaatctttt
    12361 cttaaactac caataaaaaa tccaattttt tttgtcatac gatttttcct ccttcaaact
    12421 atctataaca atcttacaaa aaataagtaa ctggagcaaa taaaaacact tactgacaaa
    12481 agctacatag aagatagatt acttattttt aacaaaaaag aagtctaaaa tcttctattt
    12541 cctaaaaaaa atcgaattta atcaagctgt aaagaaaaaa agctttacag ggtaaaaaaa
    12601 aactgttaca ctacatcttg tgaataaaac aatggaggtg gaaataaata atggcagtta
    12661 aaatccgttt aaaacgtatg ggttctaaaa agagtccgtt ttaccgtatc gtcgtagctg
    12721 attcacgttc tcctcgtgat ggacgtttca tcgaaactgt aggtacttac aatcctttga
    12781 aagaccctgc agaagtagtt ttaaaagaag acttagttct tgactggtta tcaaaaggtg
    12841 cacaaccttc tgacactgta cgtaacatcc tttcaaaaga aggcgttatg caaaaacacc
    12901 acgaagctaa attctcaaag aaataaggtg accggatatg aaagatttaa gcgacttagt
    12961 cttaacaatc gttcgtccgc tagtcactta tcctgatcaa gtcaagttag acgtcgtaga
    13021 gtcgaaagac ttttacgagt acaatctaac agtggctcct gaagatattg gccgtatcat
    13081 tggaaaacaa gggcgtgttg ctaaagcaat ccgcacaatc gtttacggtg ttcggatcaa
    13141 tgcaccgaaa aaagtgcgtt tgaatatcat cgatgataag gaaaagtaaa taaacgtgct
    13201 gtactcgaat tttcgagtgc agcttttttg tttttttatt aattgaagaa atgattattt
    13261 ggcaaaacaa gcaagaaagc gtatccttat aggaggaggg agtgaacagc caatgagaaa
    13321 gaaaatgtta tttattccat ttgctctatt gttaagcgct tgtagttcgg ttactcaaga
    13381 atcagacaat acacatctat caagcagtat atctgaaact gttaccagtc cagaaaaaag
    13441 tagtaccaaa acggctacca ccactgaaca aacaatggct acctcaaata atgagaaaaa
    13501 gactgcttta gaccagttga aagaacaaca gccaaatgtt cccatgcctc tagatgtacc
    13561 agtctcttca ggctatctca atatcgcagc gacacataca aagcaaggtt attctatttt
    13621 atattacaga acagatcgtc ccctcgggtt gaatgctgac gaattgaatc aagaaacgcc
    13681 tattgcaacc tatctatatc aatatgggtt tgcttctagt caagaaacaa tccaagtttt
    13741 gcagcccttt gagatcgaca caaatggtca gcaagtggac ttaggttctc gaatcacagg
    13801 gtatcagcaa ggtgcagcag gttcaagttt tcttgaatgg caagaaggaa actggcgtat
    13861 tcgaatccgt gggaataata tagaaggcca agatccctta ctattagcta aagaaatcgt
    13921 agcttatttg gaagaaaaca gtctaccagc acctgaacaa tttggtaaaa ttactgttga
    13981 tatgggtgat acgacgaatc gagccgtaga agtaagctgg caagaaccga aaaatgccta
    14041 tacgatcact catcaagatc ctatgtctgc gttgaagatg gcagtttcta tgaagaggtt
    14101 gtgaaatcag cgcgctgatc tgagcccatc atttaactcc aagtcacagg aagtaatcaa
    14161 aaatagcacg ccgcattttt gcataatttt ggcttaatac cttaaaagaa aactagaatt
    14221 gcctcttcac acatgctaaa ataaaacaga caaaaaagaa atggggatat aagttgactg
    14281 aatacttgaa tgtaggaaaa atcgtaaaca cacaaggtat caaaggcgaa gtccgtgtta
    14341 tctcaacaac ggatttccca gaagaacgtt acaaaaaagg aacggtgtta acactattcc
    14401 aagacggaaa agcacctgtg gaattaacag tcaaaagtca tcgcaaacat aaaaattttg
    14461 acttattgag ttttgaagga catccttcta tcaatgatgt ggaaaaatat cgggatggca
    14521 tattaaaagt gtcaaaagac aactctgtag atctagctga aaatgaattc tattaccatg
    14581 agatcatcgg tctgcatgta ttagaagatg gaactgagtt aggtaaagta aaagaaatct
    14641 tgtcaccagg tgccaatgat gtttgggtcg ttcaacgtcc gaaaaaagct gatatattgc
    14701 ttccatatat cgcttctgtc gtcaaagaag tcgatttaga agctggagtg gtccgtgtag
    14761 aaattccaga aggactgatt gacgatgaag attgatgtat tgactttatt tccaagaatg
    14821 tttgaaggtc ctatggggga atccatcatc ggtaaggctg tagataaagg attattagat
    14881 atcaatatct ctaacttccg cgattattca gataacaaac atcagacagt agatgattat
    14941 ccatacggcg gtggagcagg tatgctgctc aaagtccagc ccatctatga taacatccaa
    15001 gccattgaaa aagctgcacc ggatgtcaaa aaaagagtaa ttcttcttga cccagctggt
    15061 aaacgattcg atcagaaaat ggcagaagaa ttttcacaag aagaacactt agtattcatt
    15121 tgtggtcatt atgagggata tgatgaacgt atccgcacat tagtgacaga tgaagtttcc
    15181 ttaggagatt atgtccttac tgggggagaa ttaggtgcga tggtcatgat cgatgcaaca
    15241 gttcggcttt tgccagatgt gttagggaat cagacttcag ctcaaacgga ttcttattca
    15301 accggtttat tggaacatcc ccaatatact cgcccggcag aatataaagg gatgaaagta
    15361 ccagaagtat tgacaaatgg aaatcataaa ttgattgaag aatggcaatt aaaagaatca
    15421 ctaagaagga cctatcaacg tcggcctgat cttttggaaa cacttgaatg gacacctcaa
    15481 atgacgaaat ttttagaaga aatcaagaaa gaagaacaag aaaaaacagc agatgaagcc
    15541 aagtgacttt ttctaaaata ttctttacag taggttgata atatgttagt attttatggt
    15601 gagtgcaaaa gcacttaact attacgatat tccgctatga gaaaagctca taagaatatt
    15661 tggaataagg agaagaaaac catgaatcca ttaatcgaag aattaacaaa agaacaatta
    15721 cgttctgaca tcccagcgtt ccgccctggt gacactgtgc gtgttcacgc aaaagttgtt
    15781 gaaggtactc gtgaacgtat ccagttattt gaaggtgttg ttatcaaacg ccgcggtgct
    15841 ggaatcagcg aaacttatac agtacgtaaa gtttctaacg gagtgggcgt tgaacgtaca
    15901 ttcccattgc atacaccacg tgttgcaaaa atcgaagtag ttcgctacgg taaagtacgt
    15961 cgtgcaaaat tgtactacct acgtgcatta cacggaaaag ctgctcgtat caaagaaatc
    16021 cgtcgttaat cattgttatc agactgagaa agaacccttg gtattcaagg gttctttttt
    16081 cttagcatgt aatttgaaca ccctgatcca gtttttttct ttaattattg gtaggggaga
    16141 ataatggatt agaataaatc tacctataag tagtagtaaa aaaaagagca ctatgataat
    16201 atctttctta agagtaaccg ctttaataaa aaatccagaa tattctaaca taaatggagt
    16261 attcttagaa tcaaaaggag aaagaagatg gtttatactt atacggagca agaaaaaatg
    16321 aaagtttgag acacattcat gaatgatgga aaagtaaaac aagtaccaag ttcaacaaaa
    16381 aagaaaaaca tcctattgaa agaagtcttg aaacgctttg accatggagt gacttataca
    16441 gaaacagagg ttaattcgat actgctgaac gtattttcta gtggcgatta tgtcgaacaa
    16501 agacgttatc taatcacatt tggctttttc aaaagaagtt cagacggtcg ggcatatcag
    16561 atgatgggaa ttgaaaatta gaagttttaa tttatactca ttgaaaaaag ttcaagtatg
    16621 cgatattggt tctacaccgc atacttgaac tttttttgtc atctgtatct ataatacaaa
    16681 gccaagaacg ttattcaaaa tatggatagc tatgataaaa gctaatgagc gattcgcagt
    16741 taaataagca atcgtcagta aaatcgttcc aggagcgtac atgaaaaaat caattggatg
    16801 cgttacatga ataaagataa ttctctttta tttgtacgaa taaataccaa aacgtacctc
    16861 ctaaaattag attcaaagaa gatagcaaag aggaaaaaga tagttctgca gcagctatag
    16921 cggcatgtgg attgctagaa ctttccaaac aactgcctgt ttcagatcct cgtcatttcg
    16981 attacgaaga gatagcaatc aaaatcttgg aagaattaca gaaaaactat acaacgaaac
    17041 gagttaacgg atcaaacggt ctgcttctcc atgccgttta tgataaaaac agcttaaaag
    17101 gtgtagatga atgtgtgatc tggggcgatt acttttatgt agaaggaatc actcgtctag
    17161 ctaaaacatg gtactgttac tggtgatgta taagagaaga ggaggattca tataatgaag
    17221 cgatggaaaa aaatgatgat tggatcaata gttttaggaa tgtcactcgt atttggtgct
    17281 tgtggtaatc aagaagacag tagtgaagaa tcagcaaatg aaattaccgt ttcgacatgg
    17341 aattatgaaa caacacctga attcaaagcg ctctttgaag cattcgaaaa gaaaacggga
    17401 atcaaagtga aagcagtaga tattgcttca gatgattatg atacgaaact caccacgatg
    17461 ctttcttctg gagacacgac agatgtattg acgatgaaaa atcttttgtc gtattcaaat
    17521 tatgctttac gagatcagtt agtcgatcaa acagaacgaa ttagagaatt agacactgat
    17581 gctgctgaag aaacatatga aatgtatgat atcgatggaa aaacatatgc attgccatat
    17641 cgaacggact tttgggtact atattacaat aagaaaatgt ttgatgatgc aggaatcgaa
    17701 tatccaaaaa atctaacttg ggacgaatat gaagatttag caaaaaaact tagtaaaaat
    17761 gacggacaag tttacggagc atatcaacat atctggcgtt cgacaatcca agctattgca
    17821 gctgctcaaa acgaggcgaa tcttgttgaa ccagattatc aatatatgac tgattattat
    17881 gatcgagcat tgaggatgca aaaagaaggc gcgcagatgg attttggcac ggctaaatca
    17941 acgaaagtaa cctatcagtc tcaatttgaa gaacaaaaag cagcaatgat gtacatggga
    18001 acttggtata tggctggcat actcgcaaac aaagaagcaa gtaagacaga agtagaatgg
    18061 ggaattacag ctattcctca gaagaaaaaa ggagaatcgg tcacgacgtt tggctcacca
    18121 acagcatttg ctgtcaataa aaactcaaag aaacaaaaag cggcgcaaga atttattgaa
    18181 tttgcagcca gtgaagaagg agcaaaagta ttagcaggag taggagtagt tccttcatat
    18241 cgtacggatg agattgatca gctttatttt aatctcgctg gaatgccaac ggatgaaatt
    18301 tctaaaaaag ctttttctcc tgatgaaatc aaacttgaat ttccgattga tacgtatggg
    18361 cctgcaatcg ataagatttt acaagaagaa tatgacttag ttttggttgg agatgaaacg
    18421 ccggaaaaag gaacggtaaa tatggagaaa cgtgtagctg cggaaaaaga ataatcatga
    18481 aaggagagaa gccaagagat aaaacactct tggtttcgtc atcgaaatgg agaatagtat
    18541 aaataagaaa gctacaaatc atttgcgtag aaaacgaacg tggatcgctt attcgtttat
    18601 cgccccgaat ttcattggtt ttttcgtttt tacattaata ccaatcctgt tttcgcttgt
    18661 actagcattt atgaaatggg attctttttc aacgccagaa tttgctggac tagataactt
    18721 tcgtaaaatg gccaacgatg agacattttg gatttctttg aaaaatactt ttttatatac
    18781 cgctgctgta gtgcctggca cacttgtttg ttcgttaggg ctagctttac tactaaatca
    18841 gaaaattaga gggatgaagc tttttcgtac gatatttttc tttccgtatg ttacttcctt
    18901 agtagcaatt gctgttgttt ggaatatgtt gtttcatcca acagtgggac ctattaatat
    18961 ctttttaaga cgatttattg aacatcctcc aggttggact tcaagttcaa attgggcgct
    19021 ttgggcaatt gtaattgtca gtatttggcg agggatgggt tattacatga ttctatacct
    19081 tgcaggacta caaagtatcc aaaaagaact atatgaagcg tcttcgctag atggagcaaa
    19141 ccgttggaaa caatttttga atgtgacatt accttcgttg agaccaacaa ccttctttgt
    19201 aacgatcatg cttgtcatca attgtttcaa ggtgtttgac cttgtgcaag tgatgacgga
    19261 tggcggccct ggtcgagcga caaatgtgct ggtttaccaa atctacaatg aagcattcaa
    19321 aaaatttgat tttggttatg cttcagctat ttcacttgtt ttgttcgtaa ttgttttggg
    19381 cgttacggtt gttcaatttt cttggaataa gcgtcgtact taggaggtgt gatatggaag
    19441 agagtgttcg aaatgaaaga aaaaaagcaa aaattcgttc aaaaaacatg ccctttattc
    19501 aatggttggg tatgattctg ctgattgtat tagctttatt gacgatcatt ccttttttat
    19561 ggatggtttc tgcttcattc aaagcaaata atgaagtgtt aacgattccg attcaatgga
    19621 tccctaaaac ttggcattta gagaattatc tggttatttg gtctcgtatt cctttagtga
    19681 cattttttaa gaatacagct tttttaagcg ttgtgatcac gttgattcag ttgtttactt
    19741 ctagttttgc tgtttacggg ttttctgaac taaattttaa agggcgcgat attttatttt
    19801 tagcatatat cggcacgatt gctgtccctt ggcagtctta tatgatcccg cagttcatta
    19861 tgatgagaaa agtgggttta acagatacgt tgtggtcgtt ggtgctatta caagcgttca
    19921 atgcttttgg tgtgttcttg ttgaagcaat attacagtag tatcccagat tctttatgtg
    19981 agagtgcgcg gatcgatggc ttatcagagt gggaaatcta tcgcaagatc attttacctc
    20041 ttacaaaacc ggcgattgcc agtttgacga tcatcacatt cgttaataca tggaacgatt
    20101 atatggggcc attcatttat ttgtcttcta cggaaaataa aacaatccag ttagggttga
    20161 aaatgtttat tgggctattt gatgcagagt acgcattaat catggctgcc tcagttgttt
    20221 ctgttcttcc tgttgctttt atatttttag cgatgcaaaa atattttgta gaagggatcg
    20281 cttcatcagg aatgaaggaa taggaggtga ttatgaaaga gcagcgtttt gacaaaaatg
    20341 tttatatgga attcttttat tggttgtatc aatcattggc aagtagcctt tgtttttggt
    20401 gtgtcaatat cttgtatata gcagctgcgt tccttttgtc gatagatgga aggaatgtct
    20461 tttcctttat tttttcttta ctttttattg gtccaggttg catcgtgatg ataggtatga
    20521 cagaagaatt agcaaaagaa aaaagttttt caccagtacg tttgttcata aagacgtttc
    20581 gccaattttg gctaaaggga tttttttatt ggctatttgc ttggatagtt agtgtgataa
    20641 tgatttttga ttgtttcttt tttatacgtt tttcttatgg aaaatggttg attccgttgt
    20701 ttgttttact tgcttgtttt acttgcttgt ttatcagttt cctttagcat caactgctgg
    20761 tattttcagg tgagaaatcc agcgtcgaaa ccaaatcaag ttctacggat agcgttctac
    20821 tatacgttga aaaaatggta tgtgagttta ctagattttt tactattgac aagtttgttt
    20881 ctcttctttt tcgtaaagcc ccaatggtgt attctactag gtccttctat cgtatttggt
    20941 ttgatttact tcaacaatcg taaattgatg agaactatgg atctgtagac tttataaatt
    21001 gaaactttca attaagaatg acagtttttt atgtaaaaac tgtcattctt tttcattttt
    21061 tgaaacatat ggctaaacaa atgaaataaa acatgctata cttttgaatg aaacaggttt
    21121 agaaactgta tcacttattt gtgattattt ctaactaata attaaaaata aagcataaaa
    21181 aggacgatgt tcaatggctg aaaaaatgaa acaaggttcg aaatctgtag tagaaagttt
    21241 gattaatcat catgttgatt atgtattcgg tataccaggt gcaaaaatcg atggtgtatt
    21301 taatgaatta gaggatcaag gtcctgaatt aatcgtgact cgtcacgaac aaaatgctgc
    21361 atttatggca caagcaatcg gacgtattac aggagagcca ggagtagtta tcgcaacaag
    21421 tggtcctggt gctagtaact tagctacagg tttggtaaca gcgacagcag aaggtgaccc
    21481 agttttagca attgccggac aagtaaaacg aagcgatcta ttgaagttga cccatcaaag
    21541 catggataat gcagcactgt tccagccaat cacaaaatac agtgcagaaa tccaagatcc
    21601 agaaacaatt tcagaagtga ttgccaatgc ttatcgtatg gctaagagtt caaaaaaagg
    21661 cgcgagcttt atcagtatcc cgcaagatgt agtggatgca cctgtccaag gtaatgtcat
    21721 caagccattg agcgatccta aattaggttc tgcttctgca gatgatatcc gttacctagc
    21781 tgaacgcatt agagaagcaa aactaccggt actattagta ggtatgcgag gatcaagtga
    21841 aaaagaaacg ttagccatca ggcaattagt tgaaaagaca gcattgcctg ttgtagagac
    21901 attccaagca gctggagtga tctcacgtga attagaagcg catttctttg gtcgtgtggg
    21961 tttatttaga aaccaaccag gagatatgct tttgaaacga agtgatctag ttattgcaat
    22021 tggctatgat ccaatcgaat atgaagcaag aaactggaat gcggaaaaag atgcgcggat
    22081 cattgtgatc gatgaagcgc cagctgaaat cgatcctttt atgcagcctg aaagagaatt
    22141 gatcggtgat atctcagcca ctcttgattt attgacaggt tcattagagc ctcaacaagt
    22201 gtcagaagat gcaaaagaat atttagcatc tcttcaagca aaattaacgg agcgggacat
    22261 tgttcaatca aaaggagaag caggtatctt gcatccttta gaagttatta acacgcttca
    22321 gtcaaaagta acagatgata tgactgttac cgtagatgta ggtagtcatt atatttggat
    22381 ggcacgccac tttagaagtt atgaacctcg tcatttgtta ttcagtaatg gtatgcaaac
    22441 gcttggtgtt gcattgccat gggcgatttc tgctgcattg gtgcgtccaa atacacagat
    22501 cgtatcagtt tccggcgatg ggggattttt attctctgcc caggatttag aaacggcggt
    22561 acgtaaaaaa ttgaatatcg ttcatttgat ttggaatgat ggacactaca atatggtaga
    22621 gttccaagaa aaaatgaaat atcagcgggc gtcaggtgta gactttggtc cagttgattt
    22681 tgtgaaatat gcagaagctt ttggcgctaa aggaatccga gctacaagtg tagaggaatt
    22741 agaaaaagct ttagaagaag gatttgcgac agaaggaccc gtaattattg atattccgat
    22801 tgattaccgt gataatgaga aattgggaga aacaatctta ccagatcaat tctattaagg
    22861 aagtgtcgac atgactgaaa aaatcttata ccagcacggg acattaggag ctttgatggc
    22921 cggcttgatg gatggaacag aaacgattgc tcatatatta gaaaaaggaa ctctgggatt
    22981 gggaacattg catgggcttg atggtgaagt catcttttta gatggcgtag cttatcaagg
    23041 aagatcagat ggctcagttg cccaattaga tggaagtgaa cttaccccct atgcagcaat
    23101 tacagatttt acgccagata ctagtttttc tgtctcaaaa actgcagatg gagaacaatt
    23161 gaaaaaagac atattagtaa gagaagccgg ggaaaaccta tttttggcag tgaagataac
    23221 tggattattc aaaaatatgc acatccgtat catgccgaag caagaaaaac cttacagaag
    23281 actagcaaaa atttcagagt ctcaaccaga attccaacaa agcaatgtcc aaggaacatt
    23341 agtcgggttc tttacgcctg aattattcca aggagttgct gcagcgggat ttcacctgca
    23401 ttttatcgat gatacacata catttggcgg acatgtaatg gattttgaag tggctgaagg
    23461 aaaagttgag attagccgca tctcgtccct tgtacagcat tttccagttg atgatccgac
    23521 ttttgttcac gaaacgattg attatgctga tttagcagca gaaatccaac aagcagaata
    23581 gaaaaagaca gcgagatgcg ttcaaggtga ttaacctgac gtatctcgtt tttttgagga
    23641 gaaaacctag cgtttacaaa aaaactgagc tataatcttc tccatagatc aaaaaggagc
    23701 ggtttttatc atgtggacag aaaaacagaa aaaagatgca tacgaaattt tattattaca
    23761 gcaacaagga ttattagaag cagaagataa ctggttagca aatttagcaa attcatctgc
    23821 attgttgaat gagactttgc ctgagactgt ctttgcagga tattatttat tcgatggtaa
    23881 tgaattgatt ttaggaccgt tccaagggcg agtttcttgc acacgtatca agatgggaaa
    23941 aggagtttgt ggagaatcag ctgaaaaaca agaaacattg atcgttgatg atgtcaaaac
    24001 ccatgcaaat tacatttcct gcgattcagc cgcacaatca gaaatcgtag tgccaatggt
    24061 aaaagaaggt aatttaatcg gtgttttaga tatcgattgc ggagtaacga aagggtatga
    24121 tgaaatcgat caagcatttc tagaaaaata tgtagatctt ttattagcaa attttaatat
    24181 aaatgctttt tcatttatta ataactaaaa tcgtctattg aaacaaaaaa gaagagaaat
    24241 caagaataaa aagagccgga aagacagtgg gcatttctgt ttttcggttt ttttattttt
    24301 ttataaaatc acaagtgtta ttgattttca taatcatatt ttaatgttat tatcatttta
    24361 aataatgaaa aaacattaag gatggatgaa atatgaataa cgaaactatc attttaggaa
    24421 aaaaatatat ttgccaacct attggcttga aacaaaaagt gattggagaa gtaatcagta
    24481 aattagaaaa ttgtgttgtg atctgcgtag acgaatatca tgacgtcgat cgcaatgaaa
    24541 tattagaaaa atgcggaaaa gtagtggcaa aatatactca cgtttatgag ttggaaacaa
    24601 cagaatatct atataactat agcaacaaat atgaacatac tttagtttca taaagattta
    24661 aataaagtgc cttcaaaagc gagaagacca agcttttgaa ggcactttat ttagttggca
    24721 gtgattgttc atgatttttg atttattcaa aaggttcgcc tagagaatct gtctctgtaa
    24781 cgaggcctgg tggagaaact cgataaaaac ctatactgcc agaactgtcc tattttcgga
    24841 tagacaaact agtagtttta aaaagatcat ccgatccaat catttcaaaa aatgcaaaac
    24901 tgatatcagg atcatttagc tcaggcttgt tatcgtaaag catttgtaaa ggttgcattt
    24961 agttaggagt aaacgcgaaa ctatcatcat tatttgctga agaagatgaa gaagctaatt
    25021 cagagtttgt ttgaaataaa ggagacgtgc tgacagtttc tttaccctgt tttttgcaga
    25081 ggtagaaact tttatatcgg aagtttctac cggagaattt ttctcatttg tagaaattgt
    25141 tgaatcgata ctattcgaaa tttgcgtatc tgaaggtatg ttctttgatg cagaagtccc
    25201 acaagcagta agtaacaata aatttgataa actgataaac agaattcttt tcattatgta
    25261 cacactcctt atagtatcgt ttagcataca cctatgctca gtattaggca aaatcaatgc
    25321 aaaactatca agtcttttat agtatctatt agtcttagta atacatatat ggagagggtg
    25381 atatcccagt tcaaaccaga tacattggtt accatctgta ttaacgatta tgcctctaaa
    25441 cgcttttttt atgaaaaaat ttataagtga aaacgtaggt atgatattat ctagaggtaa
    25501 tttttttggg tatttaaata gttgctagga ggatagcttt tggattttta cagtaatttt
    25561 atcttaataa tagcaatatt attacttcta aatatttggt tttttgataa gagtagaaat
    25621 gcagggatag gttttagaac caaacgaagt acgtcttctg aaaaaaagtg ggtatattca
    25681 cagactattt tttacggagg ggttatttca atcagtttac tttcttcaac actttattct
    25741 tttaatgtta tagacgtttc catgtcaaat tttatatcta ttattggaat ccttatttct
    25801 gcaattatta cacaactact tcttgtgttt gaagagaagt caaaaaataa ttaaagcaat
    25861 tatgcctttt agtacaggaa ttattacttc ttacaaatgt atagcgaatg tgaagcctgc
    25921 tgttttaatt gttctagaaa aacttttgag tgaacaatta ttttatgtta ggatgaatta
    25981 atttctctag agttta
//
LOCUS       DACKAC010000038        25983 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.038, whole genome shotgun sequence.
ACCESSION   DACKAC010000038 DACKAC010000000
VERSION     DACKAC010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25983
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.038"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(38..634)
                     /locus_tag="I0B46_001631"
     CDS             complement(38..634)
                     /locus_tag="I0B46_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAR2083936.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            984..1328
                     /locus_tag="I0B46_001632"
     CDS             984..1328
                     /locus_tag="I0B46_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAR2083937.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVEWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            1329..1748
                     /locus_tag="I0B46_001633"
     CDS             1329..1748
                     /locus_tag="I0B46_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAR2083938.1"
                     /translation="MMTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHTDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1840..2190)
                     /locus_tag="I0B46_001634"
     CDS             complement(1840..2190)
                     /locus_tag="I0B46_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083939.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(2156..3487)
                     /locus_tag="I0B46_001635"
     CDS             complement(2156..3487)
                     /locus_tag="I0B46_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAR2083940.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGVFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSVLSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGILLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLSGIREIFDSSGKLTT
                     DFIANSGIGATLINMGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            3742..4308
                     /locus_tag="I0B46_001636"
     CDS             3742..4308
                     /locus_tag="I0B46_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAR2083941.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            4453..4540
                     /locus_tag="I0B46_001637"
     tRNA            4453..4540
                     /locus_tag="I0B46_001637"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4485..4487,aa:Ser,seq:cga)
     gene            4876..5850
                     /locus_tag="I0B46_001638"
     CDS             4876..5850
                     /locus_tag="I0B46_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAR2083942.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNINKEDLDRHELVSYPVIKEQQINYVN"
     gene            6175..8070
                     /gene="asnB"
                     /locus_tag="I0B46_001639"
     CDS             6175..8070
                     /gene="asnB"
                     /locus_tag="I0B46_001639"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAR2083943.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKLDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(8265..8711)
                     /locus_tag="I0B46_001640"
     CDS             complement(8265..8711)
                     /locus_tag="I0B46_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAR2083944.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8939..9088
                     /gene="rpmG"
                     /locus_tag="I0B46_001641"
     CDS             8939..9088
                     /gene="rpmG"
                     /locus_tag="I0B46_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAR2083945.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            9176..9694
                     /locus_tag="I0B46_001642"
     CDS             9176..9694
                     /locus_tag="I0B46_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAR2083946.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(9771..10727)
                     /locus_tag="I0B46_001643"
     CDS             complement(9771..10727)
                     /locus_tag="I0B46_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAR2083947.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10893..11699
                     /locus_tag="I0B46_001644"
     CDS             10893..11699
                     /locus_tag="I0B46_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAR2083948.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKITLLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            11696..12685
                     /locus_tag="I0B46_001645"
     CDS             11696..12685
                     /locus_tag="I0B46_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAR2083949.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            12682..13686
                     /locus_tag="I0B46_001646"
     CDS             12682..13686
                     /locus_tag="I0B46_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAR2083950.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIGLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13818..14360
                     /locus_tag="I0B46_001647"
     CDS             13818..14360
                     /locus_tag="I0B46_001647"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAR2083951.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            14380..15078
                     /locus_tag="I0B46_001648"
     CDS             14380..15078
                     /locus_tag="I0B46_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAR2083952.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            15099..15311
                     /locus_tag="I0B46_001649"
     CDS             15099..15311
                     /locus_tag="I0B46_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAR2083953.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            15311..16273
                     /locus_tag="I0B46_001650"
     CDS             15311..16273
                     /locus_tag="I0B46_001650"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAR2083954.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRYLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACANLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            16291..16686
                     /locus_tag="I0B46_001651"
     CDS             16291..16686
                     /locus_tag="I0B46_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAR2083955.1"
                     /translation="MVLWVINIILLLIVLAIIFWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16849..17214
                     /locus_tag="I0B46_001652"
     CDS             16849..17214
                     /locus_tag="I0B46_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAR2083956.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            17211..19022
                     /locus_tag="I0B46_001653"
     CDS             17211..19022
                     /locus_tag="I0B46_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAR2083957.1"
                     /translation="MKIGIIGAGPRGLSIAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNDLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSREGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGSMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLEIPLTYQDESFAEASRAFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPVIRTKELAALIDAGIFHLFEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(19084..19251)
                     /locus_tag="I0B46_001654"
     CDS             complement(19084..19251)
                     /locus_tag="I0B46_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAR2083958.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            19500..20489
                     /gene="galE"
                     /locus_tag="I0B46_001655"
     CDS             19500..20489
                     /gene="galE"
                     /locus_tag="I0B46_001655"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAR2083959.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            20501..21985
                     /gene="galT"
                     /locus_tag="I0B46_001656"
     CDS             20501..21985
                     /gene="galT"
                     /locus_tag="I0B46_001656"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAR2083960.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            22009..22989
                     /locus_tag="I0B46_001657"
     CDS             22009..22989
                     /locus_tag="I0B46_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAR2083961.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            23078..23902
                     /gene="tsaB"
                     /locus_tag="I0B46_001658"
     CDS             23078..23902
                     /gene="tsaB"
                     /locus_tag="I0B46_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAR2083962.1"
                     /translation="MYHCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDKIIVPMFDARRKNVYAGAY
                     RWKENRLEIVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFYEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            23886..24428
                     /gene="rimI"
                     /locus_tag="I0B46_001659"
     CDS             23886..24428
                     /gene="rimI"
                     /locus_tag="I0B46_001659"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAR2083963.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            24435..24899
                     /gene="rimI"
                     /locus_tag="I0B46_001660"
     CDS             24435..24899
                     /gene="rimI"
                     /locus_tag="I0B46_001660"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAR2083964.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            24896..25912
                     /gene="tsaD"
                     /locus_tag="I0B46_001661"
     CDS             24896..25912
                     /gene="tsaD"
                     /locus_tag="I0B46_001661"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAR2083965.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
BASE COUNT         8481 a         4453 c         5403 g         7646 t
ORIGIN      
        1 acattgtcaa tcaatgacta attttacaaa ataaatatta tccaagaaga ccacttagat
       61 attgacctcc ataaatcaag gccatactat ataagaaaat cgacagtggt ttagcaatca
      121 aaatgattag tgtatatcgt ttcactgaca tgtttgtcaa tccagccatc aaacataacg
      181 catcgtcagg agcaactgga agaaaaatag ctagagcaaa cagcttctca aaacggtttt
      241 gattatccag ccagccaata tatttgttat atgtcttatc gctaatcatg gaaagtatga
      301 atggctttcc ataacgacgc cctagcagaa aaatgataat cgaaccaatg cagattccaa
      361 tatagttata gataaatcca gcataagggc caaatatcag gacaccagct gcagtactga
      421 tccctccagg aatgattgga ataactactt gcaggatttg gatgaagaca aaaataatcg
      481 gtcctaaaat gatggaatca cctactaatc ctcttaacgc attcaaatct ttgaaaacac
      541 ctaaattaat aaaataaatc gtcagcgcga ttgataggcc taatccgata atggatatga
      601 aatttatcag ttttcgagag gcagcaatat tcattattca tcaactcctt agaggttctt
      661 tgttaactat actttactag aaaagcaact aactgcgcca ttttttgata tgacagaaaa
      721 caaacaattt tgtaagttca tcataagtat aaaagattga tctcaaaaaa acatccaact
      781 aaagacttat ttaataactt gtttttttta aaataaaaaa agcgttagca actatgctta
      841 aaaagcatat cgactgaata aactaagcat tggacaggct gaaagactac atttaaaatg
      901 aaatgcgaag aaacaataga aaataaagtt gccttagaac catagttgaa tcacctaaca
      961 gtcgtgaagg gagaataaga gaaatggcag aaaaacaaac cgcaggacga gatcaattag
     1021 gagattttgc accaaaattt gcagaattaa atgatgatgt tttatttgga caagtatggt
     1081 ccagagaaga attatctgct catcagcgta gtatcatcac catatctgca ttaatttcag
     1141 gaggaaattt tgaacaattg ccagctcatc tgaagattgg caagcaaaat ggcattacta
     1201 gtgaagaaat agcagaaatc attacccatt tagcttttta tgtagaatgg ccaaaagctt
     1261 ggtcagcttt tagtttagca aaagaaatct atttagaaga aaatagcgaa gataagaaag
     1321 aggcgtaaat gatgacaaaa cacgaagaaa tacacaatgg cgtgattttt ccagcaggtg
     1381 aaaaaaacga agcttacgct caagtattta ccgggcaaag ctatctgaat atgttagtga
     1441 atgatcccga agtttctgtt ggtgtaggta atgtaacttt tgaaccaggt tgtcgcaatc
     1501 actggcatgt ccatacagac ggctatcaaa tcttacttgt gaccggagga gaaggctggt
     1561 atcaggaaga aggaaagcca gcaagacgat taatggctgg agatgtcgtc gtaacgcaca
     1621 aagggattaa acattggcat ggtgcgacaa aagacagttg gtttgaacat ttggcgatca
     1681 ctgctggtaa ttcagagttt ttagaacctg taacagatga agtgtatgaa ggattggaaa
     1741 atcaataatg tcaagcttga acagctcctg aaagtagatt ttaatcgaac tttcaggagc
     1801 tgcttttttg attcagtctt ttataaatag aaaatagggt taataatagg aatgagaagc
     1861 acgtcgaaag atcttaatat tgataatcgg ctgggcatac atgatttcca cgtcatcaat
     1921 caaactacct aacagatgat aatcttcccg atcatgtcgg ggattaccta atagttcttg
     1981 atagtagatt tccatactag ggtcccgagg attgttcagc aaatcagcaa gtacatctaa
     2041 atcttctggc tgagtttctg ccgtaccctg tatctctatg aaaagccctt cctcagcaaa
     2101 ttctactcca atacgtattt cagttatttc atgggaatat aaataagtaa gaagctcatc
     2161 gatgattctt tttgataatt ctttttccaa gtgtcttttt ttcctttctg acagcggtaa
     2221 agttatctag tatcggtaca aggatacctg caacgaaacc agttgaaaaa ccactgttat
     2281 atagatttag tcctccgtgc aaagtgctga cgttatggac aagagcaata tgtagcacac
     2341 cagctattac accgtaaaaa ctgccgtaaa aaccgcttat gggcgcaaga cctgtcccaa
     2401 acacagctgc aacaagcatg ccagtactgg tgatctcatg aaaagttcca aacaaagaag
     2461 caataaatat tccgactagt atcggaaaac tgttttttaa atgacaacca aaagcactga
     2521 aacccaccgc agataaaata gcaccgatga caggtccatt taactgaccg ccaaccagca
     2581 aaacataaga agacagcatc agaccaacta agcccatgtt gatcaaagta gcaccaatac
     2641 cagaattagc aatgaaatca gttgtcaatt ttccagatga atcgaaaatt tctcgaatcc
     2701 ccgaaagccg aaaggaatta taatagaaac caattccgac tgtcagcgaa aaaaagccaa
     2761 aaataatcca aatgagagga aagtgatatt cattgaaaac caatgtattt tcgacgattg
     2821 aatagctgaa taacctcaaa acagcagtaa aagtcatcgc aatcaaccca gaggtgaatc
     2881 caatattata aagtgtgaat ccacgatgga aaactaaagt ttgagcagct aagggcggaa
     2941 gaagtatacc aattgctatg ccagctaaat tccccaataa aatacctatc agtaaaggaa
     3001 aacgcatacc aaaagtgata taactaacga ccggtgataa tacgctacca aacagtccga
     3061 tcatgatgta ctgagaaaaa ggtttattga caaatacagc gtatagatac acacctaaaa
     3121 tgattgagat agaattataa aggtttttgc caaaaaaaga gaaaccggta actgttagaa
     3181 tcgaagcaaa taagggaccg tttaaaatca ccttgtgccg ataagctaaa agcacagaaa
     3241 gtaaggtcaa aatccctaca tttatgaatg cgcttccgaa aacacctaaa gcaaaataat
     3301 ctgtcaaaag attgcttggc gaggtaagaa tagttagcaa tccactccaa ttatcagtag
     3361 gagtattaga aagcaagcca ataaaaagaa aaaaaacacc tgtaaaacca agataaaaat
     3421 actttctttt ctgtgaaacg attcgttttt gaggatgaat ataaataacg tcagtttcgt
     3481 tttccattac acaccttctt tttcattttc tttaactata acttgaaatg aaagatttgg
     3541 acaactatca tttaggtcaa aactaaaaat gtaagggaac ccattgaatt taaccaaatt
     3601 tttttaattt tgcaaaaaaa agttaaagaa gactgaaaaa aatttaaaat ataacgtttt
     3661 gagtaaaatg tttgcaaaaa atgctaaaaa tagggtacaa taaaacaaca tatactgcag
     3721 taaagaagtg ggggagtttt aatgagaaga aaagtctata ccaaggatca aattctaaaa
     3781 gctgcttatg atgttgttgc aaaggaaggg tttaaaggat ttactgcgag aaatattgct
     3841 aaaaagatgg ggatttcgac acagccgatt tatctggaat tcaaaaacat ggaagattta
     3901 aaaaatacgt tgctggattc tatctttgaa caactaaaag aagatgtttt ccctattgaa
     3961 cgtactggtg ataaaatcat tgatctcggt ttgaactata tctactttgc aaaaaagcat
     4021 catagtcttt acatggcatt gtacattgat gaacatgggg acggaaagaa aatgcatgat
     4081 ttttcataca attatttccg tgaaatagtt aaagaagatg aaagatacaa tcaattatcg
     4141 gatgaatatg tcgatgctct gcatactggt acatgggtag cagtgactgg cttagcgtca
     4201 ttgatgtcat caaacatcat gcatccaaca gaagaacaaa tcattgcact gatcaaacgg
     4261 acaattgact cgattttagc attagataat cctgaaataa tcagatagtg ttctatcagt
     4321 atgaatttga tcggtaagtc cgttggctgc aaactgtcaa tggacttttt tgctaaaaaa
     4381 agcgatttct gttatttatc tattagaaat aggcttgaca tttcgtgaaa aaagtttaaa
     4441 aatagtactt acggaaggtt ggcagagtgg taatgcaccg gactcgaaat ccggcgaacc
     4501 ggctaatacc ggcgcgcagg ttcaaatcct gtaccttcct tatatcaacg attagaggtt
     4561 gtgaagaagc tgtcctgcat caagtaataa gaacggccaa acaaaaatag cttttcctat
     4621 tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg tttgttcgga
     4681 ataaggggct gtgacaagac ttttgtcaca gccccttatt gttgtacttt taacctacaa
     4741 tattttttga ttccccctga gtactatttt ttataaatgc tagtaaatca atcttctttg
     4801 gccttttttt tgataaaaaa aaatactaga tgtaagataa aagtgtaaaa acgatttgtt
     4861 ttaggaggga aaacgatgtg taccgcaatc acttatgcaa cgaaagacca ttattttgga
     4921 aggaattttg attatgagat gtcttataat gaagtagtca ctattactcc aagaaattat
     4981 cgtttcgatt ttagaaaggt aaaaaattta gataaacatt atgcaatgat aggaatagca
     5041 gctggggtat ccaactatcc actctattat gaggctacaa atgaaaaagg gttgagtatg
     5101 gcaggactga attttcctgg caatgctgat tataaagaat tacaagaagg aaaagataac
     5161 gttgcaccat ttgagtttat tccatggata ctagggcaat gctcaaccat agacgaagct
     5221 aaagaattat tggctactat caatctagtc aatatcgatt ttagtgaaaa actacccttg
     5281 tcacctttac actggttatt agctgataaa gaaaaatcga tagtaattga aagtatgaaa
     5341 gatggacttc atttatatga taatcctgtc ggtgtgttga caaataatcc gccatttgat
     5401 tatcaattat ttaatctgaa caattatcgc tcgctatcaa atggaacacc agaaaatcat
     5461 ttttcaaacc agatcagctt gaatgtttat agtcgtggga tgggaggatt gggcttacct
     5521 ggagatctct cttctgtttc tcgatttgta aaagccacat ttactaaaat gaatgctgca
     5581 tcaggtgatt cagaatctga aagtatcagt caattttttc atatactagg ctcagtcgag
     5641 caacaaaaag gagtatgcga tactggagaa ggaaaatacg aatatacgat ttattcttct
     5701 tgctgtaacg ttgataaagg gatttattat tatcgtacgt atgaagacag tcaaattact
     5761 gctgtaaata taaataaaga agacttggat cgtcatgaat tggtcagtta tccagtcata
     5821 aaagagcaac agataaacta tgtcaattaa ttaatgaata gatccgtttg gtatcatatt
     5881 ttctgataca gtcataatta gacacgatct ttaggtcgtg tctttttttg ctgttttttt
     5941 tggaaatagc atccactaat atttttttaa cacgaagaga ataaggtagc ctcaaaaaag
     6001 aaaacatggt aaattagata ttgggacttt attttctgta aaaacagaaa ctttcatctt
     6061 ttgttttcac gtgacgaaat tgtaagagag aaaatattcg tccgctattt attctatgat
     6121 aagatgcata tgaatgaaat aaacagtaat tttatattag gatgtgaaag ttaaatgtgc
     6181 ggtattgtag gttttgttaa cgacaaggac aacaaaaaaa caattattaa tacaatgatg
     6241 gatcgaatcg ttcaccgtgg acctaatagt tcaggagaat atatcgataa acatgttgct
     6301 ttaggattca gaagattaag tattattgac ttagagggcg gtactcagcc tatttacaat
     6361 gaagatcgga caaagatcat tatttttaat ggcgaaatct ataattacca gcctttgaga
     6421 gaagaattga ttgcagctgg ccacgttttt caaacacatg cagatacaga agtccttttg
     6481 catggttacg aagaatgggg aacagaatta ttgcaaaaga tccgcggaat gtttgctttt
     6541 gctatttggg ataatgaaaa gaatgagtta ttcggtgcaa gagaccactt tgggatcaaa
     6601 ccatattatt atgcagaaat gaacggaaca tttatgtttg gttcagaaat caaaagtttt
     6661 ctgcctcatc ctgattttaa caaagaattg aacaaagaag cgttgaaacc ttatatgact
     6721 ttccaatatt ctccattaaa cggcgaaaca ttcttcaaag atgtctatcg tttgcctgaa
     6781 ggccattatt atacgtataa agatggaaaa ctagacattc agcaatattg ggatgcagat
     6841 tttgaaacaa aagaaacgca ttcccgacaa gaatggatcg aaaaaatcga cgaaaccgtt
     6901 caagcttcta ttgaagcaca tacggtcagt gatgtagaag ttggttcttt cttatccagc
     6961 ggggttgatt ccagttatgt aacctctgta ttgaaaccag atcattcttt ctcaatcggg
     7021 tttgatgata aaacctataa tgaagcgatt gaagccagaa aactaaccga actattggac
     7081 ttagataata cagcagcagt gatcgacgga gacatgtcgt tcaaagcatt ccctttgatc
     7141 cagtatcact tagatgaacc tgattcaaat ccttcttgcg tacctttgta ctttttagca
     7201 aatcttgctt cacaaagtgt gcgggtagtc caatctggag aaggggcgga tgaactgttt
     7261 gcgggatacc aaacgtatgg attccatacg aattcaaaat tcatccgtgt gattgctcaa
     7321 ggattgaaaa aattaccaaa aggaacacgc tataatttag gacgtaaaat cggaaaaatg
     7381 aaaaatttcc atggacggat ccatttatat gaatcacttg ctcctgcaaa agaatatttt
     7441 atcggtcatg ctcgagtgtt cgaagaatca gaagcatcag aagtgttaac accaaaatat
     7501 caaacagcac catctgtgga tgaaatcatg actgttcatt acgagaaaac agaaggaatc
     7561 aaagatgaag tcaataagat gcaatatgtc gatcttcatc aatggatgcc aaaagatatc
     7621 ttgttaaagg cagataaact ttctatggca agttctttgg aagtccgagt acctttattg
     7681 gatatcgaag tcatgaaatt ggctcaacaa ataccaagta agtttttatt gaatcaaaac
     7741 aacacaaaag atattttcag acaagctgcg aacaaacatc ttccggaaga atggtcgaat
     7801 cgtgtgaaat taggattccc tgtgccaatc aaagcttggt tgaaagaaga acacggctac
     7861 gaacaagtca aagcattatt cgaagcagat tttgcaaaag aattttttga tcaagaaaaa
     7921 atcatgaagt tgctagatga ccatcacgaa ggacgtaaag aagagcaaag aaaaatctgg
     7981 acaatcttca gtttcttgac atggtataaa gtctactttg tagatgaaac gattccgcaa
     8041 gcagaagcaa tcgactatgt gactgtttga tgaaaatcaa atatcaataa aaaagaggtt
     8101 ttctcctacc gtttaacact gagtgataag aaacaatttc aaaaaacagc tcttcccgtt
     8161 ttatagatat tatgacttat ggttcggagt tggcagagga acaccattgt ttcgaacata
     8221 agggggttgt gacacgattt tcgtcacaac ccctttacat atgttcaata atcgatttct
     8281 ttttcttttg tcccatcttt tttataaatg aacaaagttg attctttgtt ttgtgctact
     8341 tgctttcctt tttcgattgc ttcttttttt gtatcaaaat ggttgcttgc cttttttgcc
     8401 cctttagatt tcacgatcca ttgttcttcc tcatattcta cgatcacatc tgaatcaagc
     8461 agttttccag cacgaggatt cgtatcgtgt tcatccgtct tgcttggatt tttttctttt
     8521 tcgaaagttc gtttttctga ttcactagca tcttcatacc atttttttgc ttgatcaatg
     8581 gcaattggga tcgcacggtt atcagggtac ccttcatcta ataatgcatt tgcgatgtcg
     8641 attgcttttt ttcttgtcaa tttatctaag tttttcattg atgcaggaaa gtccttcatg
     8701 ttccatggca tattagtatt cccctttatt aaaaaacata atctatataa acaagtgtaa
     8761 gggtatttat acaaaaagaa aaagattttg tttgagaagt aaaaccgttg tatttatcgt
     8821 caagtagttt tacttaacaa aatcgtgaaa aacactagca atctagaaaa aatcatggta
     8881 agataatcaa gtctgagagt catcttagag gattcttgta aaaatgggag ggaaaaacat
     8941 gcgcgtaaac atcactttag agtgtacttc ttgtaaagaa cgtaactatc taacaagcaa
     9001 aaacaaacgt aacaatcctg atcgtttgga aaaacaaaaa tattgcccac gcgaacgcaa
     9061 agttactttg caccgtgaaa caaaataatg tttttaaagt tctcaattcc ttgctggtca
     9121 aagaattgag acttttttta tctcaaaaca tctaaaatca taaggagcgg ataatatgtt
     9181 tatttccatg tgggcacaag ataaaaatgg tctgatcggc aaaaatggtt tgttaccatg
     9241 gcgattgcca aatgatatgc gtttctttcg cgaacataca atggatcgaa ttttagtgat
     9301 gggaagaaag acctatgaag gaatggggga tctttcactt ccttatcgcc atatcatcgt
     9361 gttgacaacg caaccaaatt ttcgcacgaa agaaaatgcg gaagtaatgc attcgatcga
     9421 tgaacttttg tcgtatagtc aaacgatctc agaagatatc tatgtttcgg gtggtagtcg
     9481 gatttttcaa gaacttcttc cttacactgg tcggatctgg cgaacattga tcgatagcac
     9541 gtttgaaggc gatacgtata ttgggaatat tgatttttca gacttcgccc ttatagagga
     9601 gtatgagggg ataacggatc aagaaaatct atatgcccat cgttttcaaa aatgggaaag
     9661 agtaaaaaat agggttgctt caaaagaaga ataaaatcta tgcgtctagt ttgttaataa
     9721 gaaaaaaagc atctcacgac taatcgtgaa gattcttttt ttagttaaaa tcaatttagt
     9781 ttttctttga tatcattgac catttcctga ctggcaatag gtccgttata tagccaacta
     9841 gattcaggtc cgaattgcag tacatgattg tctgtcactg ctttaagatt cttccagctt
     9901 ggttcattga acatggcagc atctttgtca ctgttcacaa ggatgatgta gtcagcatct
     9961 aattcagcta gcttttcaga ggaaacagca gaccagtcag aagtggcttt tttggagacc
    10021 tcttccacta gatcaggaac accaaattcc agatcgtcgt atacgattct tccacttgac
    10081 cgatttttgc tgaccataaa ggctgagttg ttggtcaccc acaacactgc agctgtcttg
    10141 ttctcgattt tatctgctag ttcttctcga gttttcttga tggatttttg ataatcggaa
    10201 atgatttttt ctgcctcttt ttcttttgcc aaagctttgc cgacatcttt tagttgttct
    10261 tcccatgtga catctgttcc gttttttaca acataagttg gcgcgatttt gctatattgt
    10321 tcgtatttcc cgccttcgac agtagcggaa gaagaaatca acagcaaatc aggctcaaag
    10381 cttaacactt tctcataagg aaggtcataa gaaatcgttg ggacatcctt taaatcatct
    10441 tgcagataat cttgaatgga accactgcca acagtccact gggcgatagg tttttcccct
    10501 agtgcaatca gatagtcttc taagtaactg ccgatgatcc gtttgggcgc gtttggaatc
    10561 tctactttat gtccaagtgt atctgtgact gtatgagtac tattggatga agtcgtcgaa
    10621 ttggcagttg tagctgtttc tttatctgaa gagggggagc aagcgctcag tactccagcc
    10681 agcagcaata tgctagataa tgtgaatact tttgttttta atttcatttc tttcctccta
    10741 acataattga gaatctttct cattggttat tattcctgat atttcgaacg ctgtcaaccg
    10801 cttcatttgg ctatagatat gaaagtagcg cttgacgaaa tgttgaaaca gcgtcataat
    10861 tgagaatgat tttcagcgaa aggatgggtg aaatgacgag attaagagca gctgacattg
    10921 cagccggcta cgaaaacaaa aaaatcattg aaaacatgtc tgttacgatt cctgaaggga
    10981 aaatcactag tttgattggg ccaaatggaa gtggaaaatc tactttattg aaaagtttta
    11041 cccgtatact acctgtgaaa tcaggaaaga tcattgtcaa taactctgat atctctacat
    11101 acagttcaaa agaacttgca aaaaaaatta cattattagc acaaactagt gaacacccta
    11161 caggtatgac agtagaagaa gtagtatctt acggacgata cccttatcaa aaactgttca
    11221 gtggcatgga cacgcaagat catgaagcaa tcgagtgggc gttggatgct acaggtctag
    11281 ctggatttcg tatgcgggat atcgcaacgc tgtctggtgg acaaacccag cgtgtctgga
    11341 tcgctatggc gcttgctcag gaaacagata tcttgatctt ggatgaaccg acaacatttt
    11401 tagatccggc acaccaatta gagatcctac agttattgac tgaaatcaat caaatgaaac
    11461 aaacaacgat cttgatgtcg attcatgaca ttaatcatgc ttcacgtttt tcagattatt
    11521 tattagcaat gaaaaacggt aagttgatca cagaaggtac acctgaagaa gtgatcacaa
    11581 aagagtgtat gaaaaaaata tttgagattg atgcaacgat tgtcacgctt ccaagaacaa
    11641 ataaaccagt ttttctaacc tatgatttgt tgccaaaaga gcaggaggat caattatgag
    11701 gactatgaag aaaaatagac tcctgctttt ggtctgtttt gttcttttga ttattttttt
    11761 tgttgctctt tcaaatggcg cagcgactgt gggaaaagca gatttttggc aggcgttgtt
    11821 tgattttgat agatcaaaac aaagtcacca aatcatacga aatattcgtt tgcctcgtgt
    11881 ggcgggagca tttcttgtag ggagttgttt tgcattgagc ggtgcattga tgcaaggagt
    11941 gacaagaaat cctttagcag attcaggctt actaggaatc aacgcgggtg cttcgttggc
    12001 aatggccctt agttttgctt tttggcctaa agcttctgat ttttctgtgt ttctaatgtc
    12061 attggcaggt tcttttgctg taactttgct cgtatttggt tttttgcagt tttcgaaaat
    12121 gggcatgaat cctttacagc tgattttagc tggcgtagct attagttctt tttttactgc
    12181 tatcagccaa gcacttaccc aattctttaa tctgcagcag gatcttgctt tttggtttgt
    12241 aggcggagct gccaatatca catggaaaca attagcagta ttgtttcctt tatatatact
    12301 tgctgttttg gtttcttttt cattaggaaa agcaatatct ttgattcatt tgagtgaaac
    12361 acatgcatta gctcttggaa gtcatcctaa acgaacgcgg ttgattgttt tctgtctagt
    12421 tgcagtgtta gcagccattg ccgtttcgtt agtcggacct gtttcattta ttgggctgtt
    12481 ggttccgcca gttgttcgcg gaatggttgg aacggactat cgttatgttc tacctgtctc
    12541 ttttttagtt ggcggtggat tggttatgtt ggcggatttt acagcgagga tgatcaatcc
    12601 cccttttgaa acaccttttg gattagtcat tgcattgatt ggcgttccgt ttttactgat
    12661 acaagtaagg agggaacaac ggtgaaaaaa atatattgga cgttctattt cttgcttttt
    12721 gtacttgtca tcctgtctat aggtagttta atgattggta cgccttttat ttacctggat
    12781 cagctttttg ccacgttcat agggaaagaa tcatcttctt tggcgctgat tgttagagaa
    12841 tttcgagttc ctcgattact gatcagttta ttagcaggag cttgtcttgg cgtttctggt
    12901 tatatcttgc aaggagtaac aagaaatgaa ttggcagatt ctagtatatt gggcattaat
    12961 gccggagcag gatttttagt gatgctttat ttaggtttct tttcgcacta ttcgctgccg
    13021 tttctattgc tagttgtagc ctttctaggt ggtgttttag ctgcttgctg tgtctatctt
    13081 gcggcctatg atcgaaatgg ctttttgggt atgaatcgaa tcttattgtc cggtattgcc
    13141 gttaatgccg gcctttctgc attaacattg ctatgcacga tccaattatc taaggaaaat
    13201 tatggatttg tgaatgcatg gttagcggga tcaatctggg gtgcgagctg gccttatgtt
    13261 tgggcattgc ttccatgggc ggttatctta attccgctag gaggacttta tgcacagagg
    13321 attggattgc tatcatttgg tcaagaacgc gcgcaaggat taggccttaa cgtggctaaa
    13381 agtcaaaaaa tccttttgtt gttagctgtt gctttggcaa gcggtagtgt agcggtgaca
    13441 ggtagtttat cttttgtagg actactagct cctcatgctg cgaagctagt cgtacgtaaa
    13501 gaaaataact ggacctttgt tctgagcggt ttgttcggtt ccatttttct gcttagtgca
    13561 gatatggttg ctagagtgat tctaccaagt ggggaagtac ctacaggaat tttgattgcc
    13621 ctttttggtg cgccatattt tctgtatgca ctttttatca gacaaaaaca cgcgctgaac
    13681 agttgaaatt tattaaaaaa ggaaggccaa tcatttctag gtcttctttt tattctattt
    13741 atgcatttat ttgaaagttc gattgctatt tttctttttt tcctatacca ttttatgaga
    13801 gaaggaaggg tgagtttttg gaccaattga aagtaagatt acgccggaaa ggaatcgaaa
    13861 agctgaaaaa tctggccaaa agtggcaaga aaaaggaaaa agaacagcga atctataaac
    13921 agctattcgc cagcgagtac tggaagtcag caaaagtgat agggacgact ttatcgaatg
    13981 aatttgaact ggatacacag ccggttatcc aacgagcaga aaaagaacaa aagaaaatag
    14041 ttgtacctaa aactttgccg aaacgtcaga tggcgtttta tgaaatggat gaacagtccg
    14101 ttcttgtacg ttcgagtttt ggtgttcttg agccacaatc agagaagctc tatttgccgg
    14161 agatgatcga tctcctcatt gtaccaggta ttatctacaa tagagccggt tatcgaatcg
    14221 gttttggcgg tggttattac gaccggtatt taaataattt tcataaaaaa acatgtagcc
    14281 ttgtttttcg tgaacaattg aatgaacaat ggcaaccgga gagctttgat cagaagatcc
    14341 aacaattgtt tattgactaa aatgaaatgg gaggttttca tgaattatca acagcaaata
    14401 aaattgcgtg catggttaag acgaccgtta tggacctatg cttttttagg gatacagaca
    14461 gcggtcttcc tcatgatgga attatttccg cgacttgaga tcccttatta tgccggaatg
    14521 tatggtcctt atcttgttca cttcaatgaa tggtggcgtt tagtgacacc aatcttcatc
    14581 cattttggac tgatgcattt cgtaatgaac tcactgatac tgtattttat ggggcagcaa
    14641 atcgaggcaa tctatggcca ttggcgtttt ttcttgattt atatgttcag cgggataatg
    14701 ggaaatacag caagctttgc ttttaacgaa gcgaatgttc tgtctggtgg tgcgagtact
    14761 tcgatattcg gtttgttcgg tgcgttgttt atcttagggt ttcattttaa acataaccca
    14821 gcgatccagc agctagtcag acattttctt ttatttattg tgttgacatt tgtttttggt
    14881 cttctggata cctctgtaga tgtttgggga catgtcggtg ggctaattgg cggattagtg
    14941 ttagggaatg ttttgggtct accgaaacaa cagacaagct attcgataca ccaaaggatt
    15001 ctttctacac tagtttttgt atttcttttt gtaatatgta ttttactggg tttaaaaaaa
    15061 tatggtttac ttgtataata tgaaggaagt gcttaaagat ggagagctta tatgatgtac
    15121 agcagctgtt caaaaaattt ggcatcttta tctatgtggg tgcacgcatc tatgacattg
    15181 aattaatgac tatcgaattg cgtaatctgt atgatagccg tttgattgat catgatacat
    15241 acatgacagc gtggcgcata ttaaaaagag agcaccgaat tgaagaaagc cgaaagaaag
    15301 gaaatttgta atggacaaga agattattgg gattgattta ggtggaacaa cagcaaaatt
    15361 tgcaatttta acaccagaag gagagattca acaaaaatgg agcatcgata ccaatatttt
    15421 ggatgatggt aagcatatcg ttcctgaaat cattgaatca atcaaccatc gtttgaattt
    15481 atataatatg aaagccgaag actttatcgg aatcggcatg ggtaccccag gaagcgtgga
    15541 cagcgaagct ggaacggtta ttggtgcgta taacttaaac tggacagaag ttcaatttgt
    15601 gaaaaaactg atcgaggcag gaacaggaat caaattcgct atcgataatg atgcgaatgt
    15661 tgctgcttta ggtgaacgat ggaaaggtgc tggagaaaac gatccagacg ttgtattcgt
    15721 tactctagga acaggtgttg gcggtggtat cgttgctggc ggaaatttga ttcacggcgt
    15781 gagcggagca ggcggagaga tcggccatat cactgtggat cctgaagggt tcgaatgtac
    15841 ttgcggtaaa cgcggatgtt tagaaactgt gtctagcgct actggtgtgg tacgtctagg
    15901 tcgttacttg gcagaagaat acgcaggaga ttcaaaactg aaagcaatgt tagacaacgg
    15961 tgaagaagta acaagtaaag atatctttga aatggctcaa gaagacgatc cgtttgcatt
    16021 gatggttgtt gatcgcgtat gcttctactt aggtttagct tgcgccaact tggggaatac
    16081 attgaatccg tcaagtattg tgttaggtgg aggagtatcg gcagcaggag aatttttacg
    16141 tagccgtgta gagaaatatt ttaaagaatt tactttcccg caagtaagag aatcaacaaa
    16201 aatcaaatta gcagagcttg gtaatgaagc tggtgtcatt ggtgctgcct cattagcctt
    16261 gaaatttgct taaaaaaacg gaggcattac atggttttat gggtaattaa tatcattttg
    16321 ctattgattg tgcttgcaat catcttttgg gaagtctatc tacgagtaat ggccaagcgt
    16381 tcagcaacga ctttgacaga agaagaattt cgagaaggaa tgagaaaagc acaagtaatc
    16441 gatgttcgcg aaaaagatgt gtttgatgct gggcatattt taggtgcaag aaacatccct
    16501 tacactgttt tgaaagattc attaggatct attcgaaaag accagcctgt ctatatttac
    16561 gatcaaaaga aaagcttgag catccgtaca gcaaataaat tacgtaaagc aggttatcaa
    16621 gagatttatt tacttaaggg tggatatgac ggatggtctg gaaaaatcaa aagtaaaaaa
    16681 gtataaaaca gatgattgta tgacagcttt aaaaaccgaa tcaatcatta gattttgacc
    16741 aagatcacat gaacggcgca atggatagtt gtgccgttca ttttttttat tttgtgctat
    16801 tatctacata tataagctaa gcgaaaaggc agaaagaagg aaacaactat gatcaaatta
    16861 aaacgtgttt accttgatgc tgaaaaagag gatggctata gaatactcgt ggataggttc
    16921 tggccacgag gaatttcaaa agaaaaagcg cagttagatc tgtggttgaa agaaattgca
    16981 cctagtaatg aagcacgcaa aacttttgac catgtaccag aacgatttgc ttcatttgca
    17041 aagaaatacc agcaagaatt gattttcgaa gaagaaaaga agcaagctat ttttaaactc
    17101 agtcaactta tgaaagaaca tccagtcatt acttttgttt atggtgcaaa aaatgaacat
    17161 cagaaccatg ctgttttatt gaaaaaattt atcgaaatgg aggcatgcaa atgaaaatag
    17221 ggatcattgg tgccggacct agaggattaa gtatagcaga aaggattctc cataataatc
    17281 aggacagaca ttctcttgaa ctttttcttt tcgatcccaa tggtcctgga ggtcgagttt
    17341 ggcggttgaa tcagccaccg gagttattaa tgaattctgt ttctcaacaa gtgacgcttt
    17401 ttacagatga aacattgtcc agtggaggat tggtaacaca gggacccaac ttgtttcaat
    17461 ggagtaaagg acctgctaca gattatatcg aacagacggt aaccaaaaat aagcaactaa
    17521 ttatagaaga aatcaatcgt ctggaaaaag atcagcaatc tactcgatgt ttatacggac
    17581 tctatcaacg ctggtttttc tcaaagttgc aagaagaatt tccagcttct attcagtttg
    17641 tttattcttt agtaaaaaaa gtcagcaaaa gaaaagatgg ttatctttta cagacagatc
    17701 agcaaagcta ttctatcgat cagcttgttt tagcaagcgg tcactgggaa aatgatgaga
    17761 cagaagaaga aagagtgtgg gtagactacg caaagaaaaa tgatttactg tatcagcttc
    17821 cttctaatcc tgcagatgtc gatctttcga agataccgcc taaagaaaat gtctttttaa
    17881 gaggtctagg cttaagtttt tttgattatg tcggattgtt tacagtatca agagaagggc
    17941 gcttcgagaa cgaaaaagga aaacttgtct atcatccatc aggcaaggaa ccgattgtat
    18001 acagtggatc aagaaggggg ctcccttatt accctcgagg aagaaaccaa aagcaaggcg
    18061 gatcaatggc atggccacgg ttactaacaa aagaaaacct ggagcaatgg catcagtcgg
    18121 gatttttgac tggcgaaaca ttttttgact atctgaaaaa agacgttgag ttgttttatt
    18181 acaagaaaat catcgaagaa tatcatttgc cgattccaaa gaaaaatttt gagcatgatt
    18241 ttctctccct ctcttcagag gagtgtctcg gaaagcatcc agcactaatc ccttatagat
    18301 gggattggtc gtatttagaa attcctctta cctatcagga tgagtccttt gcagaagcta
    18361 gccgagcatt tattgaaggg cagataaaag aagcggaaaa aggcaattgc acaggagcat
    18421 ttgcttcaac atttgatgca ttaaaagact ggcgtgatcc aatccggcag gctattgaat
    18481 ggcacgtttt ttctgcaaag gaatacaaag aaatactatg gggatggttt actcatctaa
    18541 atagcttttt aacgatcggt ccgccagtca ttcggacgaa agaattagca gcactgatag
    18601 atgcgggtat ttttcatttg ttcgaaccgc catttgatat tcagcaaaag cacaaccgct
    18661 atgttatttc tctacaagat aaagaaatca gcagtcgcta tttgattgaa gctcgattgc
    18721 cacaaacaag cttttctcgt tcaagtaatc ctgtcataaa aaatttgaag gaaaacaaac
    18781 ttgttcgccc tttttcattt tcagataata caggaagtta tcattcagaa gctatagatg
    18841 ttgagcgagg tacgaatcgc gtgattgatg cttcagggga aatccagtct gacttttatt
    18901 gctatggaat acctgttgaa ggtgtggatt ggctaactgc agcagtagct agaccttata
    18961 cagatccatg gaatctgaga caggcagata aaatagcaca acttatattg actgacaaat
    19021 aaaaatacgc atgatgactt atctgttctt tcacaagtct tcatgcgtgt ttttaaataa
    19081 ttattaacga gaaacgcgtt tgcgcattgc ttttttacgg ttttcttcga tcattgcttc
    19141 tttttcgtca attggatcga tcactgtttt tttcacagaa gcgaccaaac cagcaacggc
    19201 agcggctgtt gccagacttc ctactaatat tccagataca aattttttca ttgaatccac
    19261 ctcatttatt atttgttata tctattatgg aacaaaagag aaaaaaaggc tagttttacg
    19321 tttgtttgta aacctttgca aaaaatcctt gaaaatgttt actaaatgtt ttattataaa
    19381 tttaccatat gaatttacga aattttactt tgtttgtttt caccataaaa gtaaacttcg
    19441 tttttttaaa ttatatataa tcaacaaaca caattttact aatcaaagga gtgtttttta
    19501 tgacaatttt agtactaggc ggagccggtt atatcggttc gcacgctgta gaccagctag
    19561 ttcaaaaggg gtatcaagta gctgtggtag ataatttgct cacaggtcac aaacaagcag
    19621 tacatccaga tgctcatttt tatgaaggag acatccggga taaagaattt ctacgttccg
    19681 tatttgaaaa ggaaccgatt gaaggagtaa tccattttgc tgcaagctct ttagtaggag
    19741 aatctgtcga aaaaccattg atgtatttca acaacaacgt atacggtatg caaatcttac
    19801 ttgaagtcat gcacgagttc aatgtgaata aaatcgtgtt ctcttctact gcagcaactt
    19861 acggagaacc aaaagagtct ccaatcacag aagatacacc agctaatcct aaaaatccgt
    19921 atggtgaaag taaattgatg atggaaaaaa tgatgaagtg gtgtgaccaa gcatatggca
    19981 tgcgctatgt cgctttgcgc tacttcaatg tagccggagc aaaagcagat gcatctatcg
    20041 gagaagatca tacaccagaa acgcatcttg tcccaatcat tttacaagta gctcttggtc
    20101 aaagaaaagc attggctgtt tatggagacg attatgatac accagatgga acatgtatca
    20161 gagattatgt tcaagtagaa gatttgattg ctgcacatat tttagctttg gaatacttga
    20221 aagaaggaaa tgaaagcaac ttcttcaatt taggcagcag caaaggatac tcagtcaagg
    20281 aaatgctaga agcagctaga gaagtgacag gaaaagaaat tccagcagaa atagcaccaa
    20341 gaagagctgg agatcctagc cgattagtcg cttcaagtga aaaagcgaga gaggttctgg
    20401 gttggaaacc tgaatacaca gatatcaaag caattatcaa aacagcatgg gattggcatg
    20461 tcagccatcc aaatggttat gaagaatagg ggaatgagat atgtcaatca gtcaaacgat
    20521 ttgtgatttt gccacacttg cgatcgaagc tggcggatgg atggaaatgg accgcttata
    20581 tttacagaat cgtcttttag ggatggtagg agaagatgct ctagaaagca ttgaagtaag
    20641 accagttaag gaagaatctg tagacttatt ggatcagctt gtcgctaagg caagagaaaa
    20701 tggtgttctt tcagaattgc cagcagaaca agagattttt gaagcgcaat taatggattt
    20761 tctcacaccg ccaccttcag tcgtcaatgc attctttgca cagcactatt cgaaaagccc
    20821 agaggaagca actgagtatt tctttaattt aagtaagaaa aatgactaca tcaaaacaag
    20881 agccatcgca aaaaacatca aatttccggc agagtcggag tatggtgaac tggaaatcac
    20941 aatcaattta tctaagccag aaaaagatcc aaaacaaatc gcagcagaac gcgaggcggt
    21001 aaaagtagac tatcctacct gcatgctttg catggaaaac gaaggatata gaggtcgctt
    21061 gaactatcca gcccgcacca atcaccggat tattcgaatg aatctagacg gtgaaagctg
    21121 gggattccaa tattctcctt atgcttatta caatgaacat tcgatcattt tatcagaaca
    21181 acaccgcccg atgaaaatca caaaggaaac atttgctaga ctgcttcgga ttactgaagt
    21241 cttgcctcat tactttgttg gttcgaatgc tgacttgccg attgttggag gatcgattct
    21301 ttctcatgat cattatcagg caggacgtca tgaatttcct atggcaaaag caccgataga
    21361 aaaacttatc aaactagcag attatccgca agtcatcgca ggagtcgtga aatggccgat
    21421 gtctgtgatc cgtttgcaat caacagagaa aaaattgttg attgaagcag ctaactcagt
    21481 attggaaaag tggaaagcat attcggatga aacagtatct atcagagctt tttcagcaga
    21541 tggtacacca caccatacga ttacgccaat tgcacgaaga agggacaatt actttgagtt
    21601 ggaccttgtc cttcgagaca acaatgtttc agaagaacat cctgatggga tttttcatcc
    21661 acataaagat gtccaacata tcaaaaagga gaacattggg ttgatcgaag taatggggct
    21721 tgctgtttta ccacctcgtt tgaaaccaga actagtagaa gtagagcatt tcttattagg
    21781 agaagaaaac cagatggctg aatatcatcg tccgtgggca gaacaattaa aaaaagaaca
    21841 tcctgatttg acgaaagata cggtcaccga tgtcgtacaa aaatctgtag gtcaggtttt
    21901 tgcacgtgta ctggaagacg ctggagtatt caagcgagat gaaaaaggac aggcagcgtt
    21961 tgaacgattt actgcaattt tataaacgta aaaaaggagg agagacaaat ggcaacaatt
    22021 aaagacattg cgcagttggc aggagtttct cctgctactg tttcaagagt tctcaactat
    22081 gatcctgatc tttccgttgg cttagccaca aagcaaaaaa tttttgaagc agcagaacgc
    22141 ttaaattata cgaaacatcg taaaaatgca gcaaatacta agcaaacgct tcgcctgatc
    22201 cagtggtacg acgatacgga agagcttgaa gatctttatt atttatctat tcgtctagga
    22261 atcgaaaaaa aggcagaaga agcaaacgtt actttattga aagaaacgtg ggattccatt
    22321 agtgatcttc cgacagatgg aacgatcgca ctaggaaaat tcgacgaagc tcagttatta
    22381 gaaatgaaag gatcccaaaa tgccttgctt ttggtagatt ctgacggcac ttctcaagga
    22441 atcgactctc tagttgtcga ctttaagtca agtgttcaaa aagtcattga acattttgtc
    22501 tcccataaag cgcaacatat cgctatgctt gctggaacag aatatacgaa gaaaaaccat
    22561 cagcggctaa aagatcccag aattatggca tttcaacaag ttttggcaga aaaaggctac
    22621 gacgaaggtc caattatcga agcagatttt tctgtcgagt caggatatca agcagtgaag
    22681 cagtatttaa aaacgaatcc gcatgttcct gatgcgcttt ttgcagctaa tgatgcatta
    22741 gcgattggcg cactgaaagc gattcaagaa aaaggtttag tggtaccaga agatatttct
    22801 gtgatgggtt tcaatgatgt cagtgtagcg aaatatgtaa cgccctctct ttctactgta
    22861 aaagtttata ccgaatggat gggagagcta gcagtggaga cgattcttca attaagtaat
    22921 tccaaagcac cggtttcaag aaagattatt gtggaaacag agttgattat tcgagaatca
    22981 acgaaataaa agatggaaaa gctgtgggga aatgaagtga taccgcagct tttttgtgtt
    23041 tcaggaaggg gcaaaatttg cccgccagca gataatcatg tatcattgta ctagttttgc
    23101 ctttggtatt ttcatcctca gaataaaaaa gtatgctaca atggaaggcg agaatgtcat
    23161 agcagaaagg atcaaatata tgattacatt aggaatagat actgcaaacc aaactttggc
    23221 gatcggtgtg gtagaagacg aacaaattct tggacaaata caaacaaata tcaaacgcaa
    23281 ccacagtgta acactgatgc ctgcaatcga ccaattgttt gcagatcttc aaataagtcc
    23341 aaaagacatt gaccgaatcg cggtttctga tggtcccggt tcttatacag ggcttcgtat
    23401 aggtgtaacg acagcaaaaa cgattgctta tacattagat aaagagctag taggagtatc
    23461 tagtctgaaa accatcgcgg ctaattgcat cggtgtggat aaaattattg tgcccatgtt
    23521 tgatgctcga agaaaaaatg tatacgctgg ggcataccgc tggaaagaaa atagattaga
    23581 aattgtactt gaagatgtac atatttctgc ttctgatttg ttcacacaac tacaaaagct
    23641 gaatgaagct atttatttcg tcgggagtga ttgtcacaaa ttcttctatg aaataaagga
    23701 aatattgcct agtgcgatga ttaatacagt tccagcatgg gatattccaa gtggtgtaac
    23761 tgtggctcaa ttaggtgctt tagctgaacc agttgcagag aaacaagctt ttcttcctcg
    23821 ctatttaaaa cgggtagaag cagaagaaaa atggttggag acgcacacac ctggagacga
    23881 aagttatgtt gaaaaaattt agagcgtatg ccagaagctt ttttcaagat aaaatgattt
    23941 atacagaaaa aacaatcgaa caaatcgatc gtcgttacgt actaagagaa ttgactacag
    24001 aagatatcaa agagctgctg gatgtcgaac gagaagtcta taatggcgaa ttgccatgga
    24061 caaaaagtgc ctttatatca gaattgaagt caccgcttcc tcatctatat ctaggtatct
    24121 ttgataatgg aagaatgatt ggttttatcg gttcaagagc gttcggttta gattgccata
    24181 tcacgaatat tgccgttcga ccggcatatc agcgtaaacg aatcggttcg ctgcttattg
    24241 acgaaatcga gaactttgct ataatgaatc gctgtgagac gatgtcatta gaagtgcgta
    24301 tgagtaatca agatgcacaa agactttatc ggaagttagg ttttcaagca acaacgatca
    24361 aaaaaagata ttacactgaa acaaaagaag atgcgttgga tatgataaaa cttttagaaa
    24421 atgagtgaga ttagttgatt tgcagaaaaa aagattttga tgaagcgtac ttggcagaat
    24481 gcgtatggat gattagcgaa aatgcttatc cacatggttc accttggacg aaaatgcagt
    24541 ttttggcaga catccaacag cctcactctg attatctgct tttagttgaa gataaccaga
    24601 tctgtggctt tattggatac tcgaaagtaa tagatgaagt cgagatcaca aatattgctg
    24661 ttgcagtttc agaacaagga aaagggcatg cacggcattt gttacaatta ctgattacgg
    24721 aacaaataaa agattcgctg catgtttttt tagaagtacg tctatctaat gaagcagcaa
    24781 gaaaacttta tgaatcagaa aagtttcgta tattaggtaa acggaaatca tattatcgca
    24841 atccgacgga agatgcgatc attatgagca gaaagttaaa aacggagacg atgaaatgaa
    24901 tgaagaattg attctggcga ttgaatcaag ttgtgatgaa acgagtgtag cggtcgttag
    24961 aaatggaaca gagatcttat ctaacatcgt tgcttctcaa ataaacagtc ataaacgttt
    25021 tggtggcgta gttcctgaag tagctagccg ccatcacgta gaacaaatca ccttatgctt
    25081 ggaagatgca ctggtagaag ctggtgtgtc ggcagaagat ttatcggcag tcgctgtcac
    25141 ttatggacca ggacttgtcg gctcgttgct gattggtatt tctgcagcta aggcttttgc
    25201 ttgggcacac caattgccgc ttattcctgt aaaccatatg gcagggcata tctatgcagc
    25261 ccgtctggtt aaaccattcc agtttccatt gatggcactt cttgttagcg gaggacatac
    25321 agaattagtc tatatgcaag aagatggatc atatgaaatc attggggaga caagagatga
    25381 cgcagcagga gaagcttatg acaaagttgg aagagtatta ggtctttctt acccaagcgg
    25441 aaaagaaatc gatcagctag ctcatcaggg aaaagataat taccattttc cacgagcgat
    25501 gatccatgaa gataattatg atttcagttt tagcggatta aaaagtgcat ttatcaattt
    25561 ggtgcataat gcacaacagc gtggagaaga tctagacaaa aatgatttag cagctagctt
    25621 tcaagcaagt gtgatcgatg tattgattac taaaacactt cgagcttgtc aaaattatcc
    25681 agtcaagcaa ctgattgtag ctggaggtgt agcggccaat caagggttgc gtgaaggatt
    25741 gcaggatgct ttggcaacta aactccctga ggtagaacta gtgattcccc ctcttcgact
    25801 ttgtggagat aatgctgcaa tgattggagc agcagcacat gtggaaatgc aaaaaagaaa
    25861 ttttgctagc tatcagttga atgctgatcc aagcttggtt ttatctgaat aaagaggttg
    25921 tgaaaaatct gccctgcatc aagtaataag aacagcccag gaaaaacggc ttctcatgtt
    25981 ttt
//
LOCUS       DACKAC010000039        25944 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.039, whole genome shotgun sequence.
ACCESSION   DACKAC010000039 DACKAC010000000
VERSION     DACKAC010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.039"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(85..2352)
                     /gene="gshAB"
                     /locus_tag="I0B46_001662"
     CDS             complement(85..2352)
                     /gene="gshAB"
                     /locus_tag="I0B46_001662"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAR2083966.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEAIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDGIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(2539..3537)
                     /gene="ftsY"
                     /locus_tag="I0B46_001663"
     CDS             complement(2539..3537)
                     /gene="ftsY"
                     /locus_tag="I0B46_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAR2083967.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKIEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(3549..4358)
                     /locus_tag="I0B46_001664"
     CDS             complement(3549..4358)
                     /locus_tag="I0B46_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAR2083968.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMVHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(4367..7948)
                     /gene="smc"
                     /locus_tag="I0B46_001665"
     CDS             complement(4367..7948)
                     /gene="smc"
                     /locus_tag="I0B46_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAR2083969.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLLKEVAEKETEIQKAEASLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYVQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(7968..8654)
                     /locus_tag="I0B46_001666"
     CDS             complement(7968..8654)
                     /locus_tag="I0B46_001666"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAR2083970.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(9002..10783)
                     /locus_tag="I0B46_001667"
     CDS             complement(9002..10783)
                     /locus_tag="I0B46_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAR2083971.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(10809..11723)
                     /locus_tag="I0B46_001668"
     CDS             complement(10809..11723)
                     /locus_tag="I0B46_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAR2083972.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(11750..12712)
                     /locus_tag="I0B46_001669"
     CDS             complement(11750..12712)
                     /locus_tag="I0B46_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAR2083973.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(12712..13656)
                     /locus_tag="I0B46_001670"
     CDS             complement(12712..13656)
                     /locus_tag="I0B46_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAR2083974.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(13657..14661)
                     /locus_tag="I0B46_001671"
     CDS             complement(13657..14661)
                     /locus_tag="I0B46_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAR2083975.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            complement(14929..15171)
                     /gene="acpP"
                     /locus_tag="I0B46_001672"
     CDS             complement(14929..15171)
                     /gene="acpP"
                     /locus_tag="I0B46_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAR2083976.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            complement(15293..16294)
                     /gene="plsX"
                     /locus_tag="I0B46_001673"
     CDS             complement(15293..16294)
                     /gene="plsX"
                     /locus_tag="I0B46_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAR2083977.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            complement(16343..18379)
                     /gene="recG"
                     /locus_tag="I0B46_001674"
     CDS             complement(16343..18379)
                     /gene="recG"
                     /locus_tag="I0B46_001674"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAR2083978.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            complement(18496..20178)
                     /locus_tag="I0B46_001675"
     CDS             complement(18496..20178)
                     /locus_tag="I0B46_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAR2083979.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            complement(20230..20592)
                     /locus_tag="I0B46_001676"
     CDS             complement(20230..20592)
                     /locus_tag="I0B46_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAR2083980.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            20862..21050
                     /locus_tag="I0B46_001677"
     CDS             20862..21050
                     /locus_tag="I0B46_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAR2083981.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            21288..23957
                     /locus_tag="I0B46_001678"
     CDS             21288..23957
                     /locus_tag="I0B46_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAR2083982.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(24048..24668)
                     /locus_tag="I0B46_001679"
     CDS             complement(24048..24668)
                     /locus_tag="I0B46_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAR2083983.1"
                     /translation="MFLINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKA
                     MHMVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPG
                     IEKRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTI
                     IKITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            24780..25181
                     /locus_tag="I0B46_001680"
     CDS             24780..25181
                     /locus_tag="I0B46_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083984.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANS"
     gene            25316..25654
                     /locus_tag="I0B46_001681"
     CDS             25316..25654
                     /locus_tag="I0B46_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HAR2083985.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT         7026 a         5500 c         4644 g         8774 t
ORIGIN      
        1 gaaaaaacac ataaattttt acatgcaaaa aagcttttga tcagattgac atcaaatagg
       61 acaatctgtc aaaagctttt tcttttaaaa tacttcagga tataacaatt tcaatacatt
      121 catcgtcaat cttctgcctt ttcctgcaaa gggataaaca tgcatatgca tggctggatt
      181 gaaatttgct tcgatgatcc catatgcttt tttatcagtt gtaggatcga tttctttatc
      241 tggtataatc aaatcgatac cactaatctt agctcctagt gcttctaccg cagaaacggc
      301 gatttttttg taagcttcag aaaattcatc agtcatatcg atcgaatcac cacctgtact
      361 gatatttgag ttctccctta gataaacaat ttgattggct tggggaactg attcgatcgt
      421 caacccttgt tctttcaaca tcaattgttc taattcacct aattggatca attctaaagg
      481 tgcacgatga ttcgttccac gcaaagggtc actatttttt tcttcaacaa gttctttcac
      541 tgttcggata ccgtcaccaa taacattagc agggactcgc aacatgatgg cttggacttc
      601 tccgtcgatc acaaagaaac gatattccgt tcccggcata aattcttcta ctaaaaccga
      661 actgtcttca cgaaaagcaa tggctaaacc tgcttgataa tcttccagac tagctccttc
      721 cttgaaaatc gtgataccta acccgtaatt ggttgattta ggtttgatca caaatgcctg
      781 ctcggcaaat ctcggataag cttttaccgc ttcttccatg gatgaaaatt ctgcgcctcc
      841 tggtacccgg aacccagctt cttttaagac ttttttcgtt actgttttgt tttccatgat
      901 aagtgggacg atatagctgt ccttacttgt catgtttgca tttttcacat attctacatg
      961 gtcttggaat tgcagacgca aaaattgatc cgactcatct aatactttga cttggacacc
     1021 tttttggatg gcatcgaaca taaatatctg agtagaaagt tccatttcgc gaaaaccggc
     1081 caactgatag ggacgttcat ggctttcttt ataatattga tgtcccaatg ccacagcaac
     1141 ttcttgctga ctcgatagct gtgcttcttg atagagtttc gctgctaacg tttgttcagg
     1201 atgatccatt tgagtcaatg cttcctctac taaataaaga gaatccaacc aatccaattc
     1261 aaccagcatt tgcttcatcc cttcaagaat ttctctacct tcttgatgaa attctgtttg
     1321 atctgatggt tgttctagtg ctactttgtt atttcgagtc gtaccttctg ctacccattg
     1381 gtcacagtct tctttttctt ctgtccataa catgtacatc aggaataatt gtaagaaccg
     1441 aacttgctct ggattgatac ccaatcttgc ataaggattc aaatcgatat tccgcaattc
     1501 aatataacga acacctgttg ttgctaaatc tgcgactttc tttccgcctc ggaaacgaag
     1561 cggtgtataa aattctttct cctccgaaag tttcccttct tcgatcattc gctcgatatc
     1621 tgctaaatac tgtttcatag aagcatagga aactttcaca tttggatgat tcgtatagcc
     1681 cagcgcactg ttacgaatac tgcgaacagg ttcttgcgga tgtgattcat ctaaaaagta
     1741 gttcttctca ctcattggtg aagcaccgaa taaataagtg atcatccagc gatagcgcat
     1801 aaagtttctc gccgttttca aataaagttc tgttttgaat tcggaaaaat cttgaaattc
     1861 ttcctgatgg ctgaacaatg ttcttagcaa ctcatcgcca aattcaaaat tgaaatggat
     1921 accgcttacc atctgttttc gcttcccgta ttcttttgct aaataacgtc gatacaagac
     1981 atcttcgaaa ttttttaatt ttgcaataat gatcgcttcg tctttttccg gtaaggcagg
     2041 tggcatactt aatggccaga tcatctcttc tttcggtata gaacgagcag tcacatcata
     2101 aacagcagcg agatactgaa ataattcggg aatagaatcg gtaacaggag tgatcatctc
     2161 tgtttgtgtt tcgctaaaat ctgtttggat ataggggtga taggatcgat cgccaaagat
     2221 tgctggatga tcggtttttg ctagatttcc tgcaagatca acccgctgtc cttcccgttc
     2281 tataccaaat cgagcttggt cgataaaagg gcgcgcattg acatgcaata ataattgctt
     2341 aaaattcatc attaagtgac ctccaattca tatgaatcta attataatca aaaagtctaa
     2401 gaaaaagaac gaaattgttt gagaaccgct tacaaataag tataaaaatg aaggcatcaa
     2461 aaacacagct atgttaaaaa caatcattca aacaaggagc tgcaactttt gtcacagctc
     2521 ctgcttttta gacatgggtt attcttcttt tagtaatcct ttgaacaatc caacgacaaa
     2581 atcatttgga tcaaacggct ccaagtcatc gattccttca ccaagaccga ccaatttcac
     2641 tggtaaatgc aattcattac ggatagcaag tacgataccg ccctttgctg taccatctaa
     2701 ttttgtcaaa acaagaccgg tcacatctgt tgtttctttg aattgttttg cttgaaccat
     2761 cgcgttttga ccagttgttg catctagtac aagaagtgtt tcttggggtg cttctggtaa
     2821 ttcccgctgg attactcgtt ttattttttc taattcgttc atcaagttca ctttattttg
     2881 cagtcggcct gcagtatcaa ctagtaaaac atctgcctgc tctatctttg ctctttccaa
     2941 agcatcgaat acgacagctg ctggatcgcc gcctgcattg ccacgaacaa cttcaacacc
     3001 agcgcgttcg ccccagacaa ctaactggtc gattgctcca gcgcggaagg tatctgctgc
     3061 tgccatcaat acttttttac cttcgttttt gaactgatgg gcaagtttac caatacttgt
     3121 tgttttccct acaccattaa caccgacaaa caaaataact gttagtccgt cttcttggat
     3181 atttagttca tttacttcat tgattccttc cgcttcatag agatccacaa gtttctcgat
     3241 gatcgcattt tggacttctg ccgatttttt cgcatttctg agtttcactt cttgacgtaa
     3301 tgcttcggtg attcgtatcg ctgtttcaaa acctacatct gcaccgataa gtgtttcttc
     3361 taattcttca aaaaagtctt catcgactgt tcgaaaattg gcaaacaact cattcatccg
     3421 ttcaccaaat gttttacgcg tcttttctaa acccttgtcg tacttctcct gtacttcaac
     3481 ttcttctgtt ttctcttcgc cagtaaaagc acgcttgatt ttatcaaaaa atcccatttg
     3541 actcccccct actctaatac aaatttttgg atcactttgg ctacgccatt ttcttcattg
     3601 ctggctgtca cgaaatctgc tgcttctttg acgaaaggca cagcattctc catcgctacg
     3661 cctagtccag cgtattggat catcggtaaa tcattttctt catcaccgat tgccatgatt
     3721 tcttcttgtt tcaaaccaag atcttttgcc aataaagaaa taccataagc ttttgtaaca
     3781 ccttttggca taaattccaa aagcatactt ctcgttttga tgatctcata catttcatgg
     3841 aattctgctg gtatttcttt gattttagga tctaattctt cttggtcata agcgattact
     3901 gccttattaa gcacaaattc atctgtaata tcgcttatat ccgctggttg gaattgcagc
     3961 agcttattca acacattgta caaggattgc ttccttgggg cactaggcag ctgtaaaacc
     4021 acattgtcag acaaaacatc tagtggcaga tccaattgct gagccagttt gtataagcgt
     4081 tggatatctg atacttccat cactttcttt tccatgattt caccagtatc atttttttgt
     4141 accaaaccgc cgttgaaagt gatgctgtag tcaccttcat ctaccagtcc taattcttgc
     4201 agataatgca ccatcgcagc taaggggcgt cctgtacata gaacgatctt gaccccttgc
     4261 tgttttgctt gtgctaatgc ttgtttattc tcatctgaaa tcagcttttg ttcatttaat
     4321 aatgtaccgt ccaaatcgat tgctactaat ttgatcattt tcgttcctac actttcgttt
     4381 cgttgatttt cgcctcttca ttgatatctt ccatccgtac agaaacgatt tttgatacac
     4441 cggattcctg catcgtcact ccgtacagta catctgctgc gaccatcgtt cctttgcggt
     4501 gtgtgactac gataaattgt gtgtcatctt ggaaatcgct caagtaacga ccaaaacgtt
     4561 tcacgttggc ttcatctaac gctgcttcta cctcatctaa gatacaaaac ggaacaggac
     4621 agacacggat aatagaaaag agcaacgcga tggctgtaag tgctcgttct cctccagaaa
     4681 gcaaacttaa actttgcagt tttttgccag gaggttgtac ttcaatctca atacctgttt
     4741 tcagcaaatc agttggttct gtcagtacta attcagcttt accgccgcca aacatatttg
     4801 gaaagacaat tttgaattct tgacggatag cttcaaatac ttctttgaat cttgtccgca
     4861 cttcatcatc catctcatcc atcgtttcaa acaattggtt tttcgctgct aacagatcat
     4921 ctcgctgtgt tgctaagaat gtatgtcttt cactgacttg ttcgtattgt tcgattgaat
     4981 ttaagtttac tggtcccagt ttttcaatct gttcttttaa ggatgaaacc tttgttctac
     5041 tgtcttcgat atcggttgtt tcttgatagt cttgggaagc tttctcaaat gtcagctgat
     5101 attctgactg cagataggtc aaatgattat ctaaaatcat ttcagctcgg tctttttgga
     5161 cttctaattt ggattgttcc gacagtcgtg ctttttgctc acgattacgc tctgccaaga
     5221 ctgcttcaag cttatcgatc tccttttgcc ttctatctcg ctgctctttg gcttttgcca
     5281 actcggcttt caatgtttct cgctgagcag ataattcgtt aatttgtttt tccaaacttt
     5341 cttctgtgac ttcatgatca gaaaagtctg ctgtcaatgt cgccaactgc ttttctatcg
     5401 cttcttgacg ttcggttgcc tctgcttttt ggactcgggc accgcggagc tggatctgca
     5461 gatgattaaa ctgctctttc aagacagcaa gatctgcttg ttcttgtgct ttttgacttt
     5521 gtacttgtgc tcttctggct tccatctggt cgctttcttg acttaacgat ttgatttctt
     5581 cgtcgatttt ttgtcgttgc gattcaagat ctgtttgttt gtctttcaat tcgttcgttt
     5641 gcttttcgta atcttcgata aactgctgca gttcacgtgt ctcaaaagtt gagatttgct
     5701 tttctttttc aaaacgttca agttcattta tgatgttttg cagctgattt gtcgcttctt
     5761 gttcttcaaa acgtaattgt tcgcctcgtg tacgcaaaac ttcttgttct tcggctaaac
     5821 gagctgtttc ttgttgcagc tcctgaactt ttttttcttg tgcttggagt tgtttatctg
     5881 cttcttcaaa ttcactagtc aactgtttca attcttgatt ttgtacaaac aaactgcctt
     5941 ggtttcctcg tttattcgct ccacctgtca tggatcctcc ggcattcatc acatcacctt
     6001 ctaaggaaac cactcgatat tgatagcgga tcgtttgcgc gattgcattg gcactagtta
     6061 agtctttggc taaaaggatc gttcctagca ggttttgaac cacagtttgg atttgatcgg
     6121 gataactgac ttgttcactg gcaatcccta taaacccttc tacagctgct gcttgtgtca
     6181 atatatgagc cggtaattgt cttggtttga ttgtcgtcag tggaaggaat gttgctcttc
     6241 ctcctctttg ctgtttcaaa tacgtgatgg cttgccgagc atctttttca ttttcaacga
     6301 tgacatgctg ggctgcccca cctaaagctg tttcgatagc taacgtgaaa tctgcaggaa
     6361 catcgatcag ttcagccact gctccgacaa tacccgaaag ctgctgttta tgctgcaaga
     6421 ccaatcgaac cccttggtaa aaaccaaaat agttttcttg gatttcttga aggcttttct
     6481 tacgagcacg tagctgctgg acttgattca ttaattgata cattttaggc tgttcttttg
     6541 ctaatgcttc ttggaacttt ttcccgtttt gttggatcat ttctaacttc tcttttgttt
     6601 cagttaaaga tgattttagc tgcgcctgct tttgtgtcaa ttcttctttt tgtaagatca
     6661 atcggtctac tgatgcctct acttccgatt gcttggctaa cgtctgctta ctttttgctg
     6721 tttcttgtac atattgacgt tctagatact tcagttcgtt tcctacagct gcttgctctt
     6781 gcatcaaatc aacatattga tctcttagtt ctgccagtaa ttctttcgtt gatttttggt
     6841 atttttctaa ttcttgctgt gtttttatga gacttgcttc agctttttgg atttctgttt
     6901 ctttttctgc tacttctttc aacaagcttt cttgaagctt ttcaaaatgt ttaacctttt
     6961 tttgtgcttc tgctaatgaa gtttgatatt cttgcgagct tttttgtgta tgctttgtcc
     7021 gctcctgcaa aacatctttt tgtccttctg tctgcttcag tttttccgaa agatctaata
     7081 atacttgctg atttttttcg atcagacgat cggcttgcgc attttcttta cgctgcttcg
     7141 ccaaaatgga ttcttgttct tggatcgact cagatagttt gcctaattct agattgaatt
     7201 tcgcaagctg tgcttgtttg ttgtcccaat ctttttttgc tgtcttgatt tcggcaacca
     7261 tcaaagagac atcggtttga gtcagtgttt cttttagccg taaaaactcc tttgctgcct
     7321 cactctgggc ggccaaagga gttaactgtt cttccaattc gtggatgatg tcttggacgc
     7381 ggctgaggtt gtcttctgtt tcaaaaagct tctgttctgc tttctttttc cgctgcttgt
     7441 atttcaagac accagctgct tcttcaaaga tcccacgacg gtcttccggt ttactactga
     7501 aaatcgcttc tacttttcct tgagaaatga ttgagaacga ttcttttcct aatccagagt
     7561 ctaagaacaa ttcttggata tcttttaatc tgcagctttg tttattgatg aaaaactcgc
     7621 tttccccagt ccgtcgataa cggcgggtta cactgatctc attgtactcc aatggcagat
     7681 aatgatcact attgtctaaa atcactgtga cttctgcaat attcagctgc ttacgggtat
     7741 cggaaccggc aaagatgata tccggcattt ttccgcctcg cagactttta gcagattgtt
     7801 ctcctaatac ccagcggata gcttctgtaa tattgctttt tccactgcca tttggtccaa
     7861 ccactgctgt gacgctattt tcaaagtcaa tgactgtctt gtctgcaaat gatttaaaac
     7921 cggctatttc aattcgtttc aaatacaaag ctttccctca cttactttta cagtgctgcc
     7981 aatgcatttt ccgccgcagc ttgttctgct aattttttcg attttccttg tccagtgcca
     8041 atcaattgat catctacata aacttcgatc caaaatactc gttcatgagc tggaccttct
     8101 tcattgatca atcggtattc gattgataca tcacctgatt tttgcaagac ttcttgtagt
     8161 tttgttttat gatccatctc atgtgaaaaa gcacctgctt tgactttagg aaaaatcact
     8221 ttttcgatga atgcatgggt cgtgtcgaat ccctggtcaa gatataacgc gcctaaaaaa
     8281 gcttcgaata ggtcgcaaag caatgctggt cgcgtacgtc ctcctgaatt ctcttctccc
     8341 ttgcccaaca aaacatattg atcaaagtga cattctttgg caaatttcga caagctgtct
     8401 tcgcgaacga tcgcagctcg tgtttttgtc aattttcctt ctggcaaatg aggatataaa
     8461 cggaataaat actgagaaac catcagttct aaaacggcat ctcctaaaaa ttcaagtcgc
     8521 tcattatccg ataattgcag attgcgatgc tcattcacat aggatgaatg ggtaaaagct
     8581 tgttcaagca aatttagatc gtgaaaaacg atgttatagt tttcttttag ttcttttgtc
     8641 agctggttgt ccattttcca cattcctttt atggatattt tttcgatact tccttattat
     8701 actggttttt tgtcaaattt gaaatggttc ggtcaaaagg taagtaaagc tttaataatc
     8761 ctagtgattg acgagaagtt gtgaaaaatt cgtttagttt cgagtatgaa gaaaaaattt
     8821 tgtgaaaatg gctttccata tttttgcata aatttgactt aataccgaga aactggactt
     8881 tgaacaccgt ttattggaga agtcatgaaa tcagcgcctt gacctgaata ttagagaaat
     8941 aaacagaaaa atatcaaaaa accgaaaaag gacatcgccc tttttcggtc atttttagtg
     9001 attatgaacg tgaatctgct gtaacagaaa catttgccca tgggtttggt acatcataag
     9061 accaatccca gtctttgaca cggtcgttga ctggcaatac ttcattacgg aacaacgttg
     9121 gaatcgcaaa tgcttcttca aatgcatatt gttgccattt ttgatatgct tctttttggt
     9181 actcttcatc aaatgctttg cttgaactga tatcgttgat caattttgta ttttcttctg
     9241 attcataacg agtgtagttg aacgcagcat ttggtcccca tagtccactt ggatttggat
     9301 cagttcctgt attccaagca gcttggtaaa catcaatttc aggatcatca ttttctaatt
     9361 tatcatagaa agaattgaat tcgatcagac gacctgttgt gtattggaca tccaaaccga
     9421 tttctttcca ctgttggata tagtaatctg ccaatggttg cgctgtttcc ccaccggaca
     9481 tcgaagcaaa gttgattgtt agtttatttc catctttgtc ttctcttaaa ccatcgtcat
     9541 ccacatcttt gtagccagca tcatctaata gttttttcgc tttgtctaga tcttgtgtga
     9601 atcctttcat ctcatcatca tggtatgact ggaatactgg aggaatcaat gaagtcgcat
     9661 tgcttctcaa acctgcatag aaacgctcac ctactgcatt attatctaat gcatagccca
     9721 ttgcttgacg taatgattta tcagccattt ttgaatctgg attgtattcc acactctttg
     9781 tatctgcatt ccatttacct aacttgaatc ctagataagt ataagaagtt tgttcacggc
     9841 ctaagtttgt atagccagca atttctttat aggtatcgta agtatctgtt ggcatttgga
     9901 aaatcatatc atataattta gagttcaatg cttcagttgc acttgccatc ggtacagatt
     9961 tcatcgtgat ctttttcaat ttaggcgtac cattccagta gtgctcgttt ggcaagtatt
    10021 ctactgattc tcctgcaacg atattactca tgtaataagg gccaaaagtc actggatttt
    10081 tacgtacttg atcgcttgat tccatatctt tgattgctac accttctaac gtatgttttg
    10141 gtgctgcata tgaccaaata ccaccacccg attgaagcat gcttgggttt acttctttat
    10201 attcgatttc aactgttttg tcgtctattt ttttgattcc ggagattgtg tcggcacttc
    10261 catcatgata ttcttccata cctacgatgt tacggaaagt gtcgtcataa cggataccag
    10321 tgtaatcttt attcccaatg atttcgtaag ggaaaatcac atcgtccgcc gttacatctt
    10381 caccatctga ccatttgacg ttgtctttta ctttgattgt cgcttttttc gtatcttcat
    10441 ctagatcaag cgttgcagct ccgtcgtctg tgattctgaa atcgtcatcg tttgcaaata
    10501 gcggttcatg agaaggagcc ataaaagctg catcatatga atcttggtaa aactcccact
    10561 ggaacaatcc tttaaactga ctgtccgttg cgaccgcagc ttctaattcg ccgtcttcga
    10621 ttgcatcatc attatttttt gttgcaattg gaaaagttaa ttcctctttg ttattgccgt
    10681 tgcctccaga gttgtttcca gaggtatctg tacctccgcc cccgcatgct gcaagaacga
    10741 cagcagacat cagcgctgca aatccaaaaa tcgttttctt cattcctatt tcccccttcg
    10801 cttctatact agcctaaccg ttggcgcgca tctgcagaac gcttgaatgc ttgtccaaca
    10861 taattgatac tcaacatcaa aactaaaata aggattgatg cgggtaacca gatccattgc
    10921 cgattcacta agacttctcc gttacttgca taaccgatca atgtacctaa actaggtaca
    10981 tttgccggca agccgaaacc taagaaagtc aaagttgtct cgataccgat gtttgccgca
    11041 aagttcatcg tcaaattagt aatgattaat gaacttagat tcggcatgat ttcgcggaac
    11101 atgattttaa aatcattggt tcccattgtt tttgaggcac tgacataatc tcttcttgct
    11161 tcagataatg tcttgcttcg gaataatcgg gctttagcta cccaataaaa tgcactcatg
    11221 atgaatataa aggaccagac attgtatctg gaaataatcg atacgaagac gataatgatc
    11281 agcatgatcg gtagaatcat gataaaatca acgatccgca tcaagatgtt gtcaatgatc
    11341 cctccgtagt agccagagat gatccctaat ccaaccccga tgatcgaagt aaggatcgtg
    11401 atcgcaaatc cgatcgtaac agagtttcta gcacctatga tcaattgtcc taatacatca
    11461 cgtccgcctt catctgctcc cagaatgaac ttttgtcctt gtgtcgaaac tgttcctggt
    11521 gcagcgtatt tgtcgaagat actaacatac atcactttgt cttgatcggt cagcatcgca
    11581 ccgataaaga tcactaataa taaaaccact agcaaaccta atgaaaaaat agcaatttta
    11641 tcttttttaa attctcgagc aatcattcgt atccccatag gaggaatact ttcttgaact
    11701 gctgtattgt ttttttcact catcgatcca ttctcctttc tgattggttt tattgaatcc
    11761 gaatacgcgg gtcaacgata ctcatgatga tatctgaaag taatgtcccc actagcgtcg
    11821 cgattcctaa aatcaatact aaggatgtga tcacggtata gtcccgttgg ccgatggaat
    11881 caataaatag ttttccgact cccggataac caaaaatctt ttcgataact acagaaccag
    11941 caatcaacga agtgatttca taccccattt gagaagccat cggcaacgct gcatttcgga
    12001 aaatatgtcg actgaatact ttattcgtcg gtaccccttt ggaacgggct gttctgacaa
    12061 aatcgagcga tttcgaatcg attacttcac ttcgcagata ttggattgtg acggcagtcg
    12121 ccaaaaatgc ttgggtaatt gatggtaaca gaatatattt caaacggtct atccattgag
    12181 tgataaatcc accttgcaaa cctagtgttt gtgatccact tgttgggaac cagcctaaac
    12241 gataaccaaa aacaaatagc atgatcaagc cgaagataaa taaaggaaca gcaaaactaa
    12301 agaagttgta gattacgaca atcttatcca agattgaatt ttgataccgg ccagcaagca
    12361 tccccagagg cacagcaatc aaataagtaa ggacaactgt caagatcgat agatataaag
    12421 tgttgatgat ccgttcgccg atcaagtcag caacagagcg tttgtataag aaactttgac
    12481 caaaatcgcc ttgcaaagca tttccgatcc aacggaagta ctggacatac catgggtcat
    12541 tcaagccagc tgcttgtcgc atctgttcga tcactgccgg atcctgattt ggattgatca
    12601 aaccggtaaa tggatccccc ggcatcgctt gggctaataa gaagatcaga acacttaaaa
    12661 taataacttg cgggatcatc aataaaacac gtcgcagaat cgttttccac attagcttgc
    12721 acctccatct ttcaacgcca ctttgtgtgt agttgtgatc gtacgcaaat cgtagactcg
    12781 accagtcgca tcatagtatt ctttttggtt ttggatatac tcttgttcta ctcttcgtcg
    12841 attttctttg tggctttccc tattttctac atcgatttgc ggaatagctg aaagcagacg
    12901 tttcgtatag atatgtcgcg ggtcattata aatgtcttct cttgtaccga tttctacaaa
    12961 tcgacctttg tacataattg cgatattgtc gcacatgtgt tttactacac ctaaatcatg
    13021 tgaaatgaat aaatagctga ggccgtattc ttgctggatt cgtttcatga aattcaatac
    13081 ttgcgcttga acagatagat ccagagcaga tacaggttca tcggctacga tcagttttgg
    13141 attggtagca acagcacggg ctactcctag acgttgacgt tgtccgcctg aaaattcatg
    13201 aggatattta tatagcgcat cttccggcat cccaacgata tctagcagtc cttttacctt
    13261 tttcttttct tcttgatcgc ttaaccgttc aaaatttcgg atgggttcag caatgatatc
    13321 caacactcgc tttttaggat tcaaactgga cattgaatct tggaagatca tctgcacatc
    13381 cttgttataa cccatttttt tgcggttgct tcgcttcgtt acatcttttc cttcatacat
    13441 gatttgtccg gctgtcacct tttctaatcc cacaacggct tttcccgtag tagattttcc
    13501 agagccagat tctcccacta aaccatatgt ctttcctttt tcgataataa aatccacacc
    13561 atctacagca tatacatgat ccgttactcg attgaagaat ccactgcgaa tagggtaatg
    13621 aactttcagg tctttgattg tgattaattc tgtcatctac acctctcctt tttcgtctct
    13681 aaagtggaaa tgcttatagc aggagcaacg taccaagtgg ttcggtgcga cttcatgaag
    13741 gataggctct tcttcatgat cgcttgctgg tatccatgga atacgtggtg caaaccgaca
    13801 acctttacga ggcatgtttt tcagagaagg aacgacacct tcgatcacat ggagttctgc
    13861 atcatgattt ccttcttgtg gtatagagtt cagtaaagag cgtgtataag gatgcttagg
    13921 atgatcaaac agttctttta cacctgctac ttcaacgaac tgtccgccat acatcactgc
    13981 cactcgatca gccatttctg ctacgactcc taagtcatga gtgatcaaga tgatccctgt
    14041 ttttgtttct tcctgaaggt ctttcagtaa atcaagaatc tgtgcttgga tggttacatc
    14101 tagggctgtc gtcggttcat ctgcaatgac gattggaggt ttgcaggcga ttgcgattgc
    14161 gatgatgaca cgttgtctca taccgcctga tagttcatgg ggatattggc gtccaactct
    14221 cgcaggatta ggaatcccta cttgttctag caattctagt gcacgagctt gacgctgttc
    14281 tgcattcatc tttgtgtgat aggaaagtcc ttctttgatc tggtcttcga ttctcatgag
    14341 aggatttaat gcagacaacg gatcttggaa aatcattccg atatcatttc cgcggatttt
    14401 attatagagc gtttcgttca ggtcgattaa attcagatcg ttatataaaa tctcacctgt
    14461 aatttttgtg ttattaggat caagtaatcc cataatcgta gtagccaagg tacttttccc
    14521 gcacccagat tccccaacga ttgctaaaat ctcgtttttg tctaaagtaa atgaaacgtc
    14581 atctactgca tcgtaatagt catctttgag tcggaatcct gtatgtaggt tctgaacttc
    14641 taataattgg ttatcggaca atcgaatacc cctctctgct tcctaaatgg aagtttttct
    14701 taataaaaaa ggacattaca cagaataaag cgttttataa caaaggttat tcttatcttt
    14761 gtgtaatgaa ttattaataa ttatatagaa aatcgcttta ctttgcaaca taatttctgt
    14821 ttatcgccag aattatcaga aaaatgactt ttacgtgtca aaaagattaa aaaaagaagg
    14881 ataaaaagct gtatgcagct ttttatcctt ctttgctttt atttgaaatt atttaagatg
    14941 gctgctgata taatctactg cgccaccgac tgtttcaatt tgttcagcgt cttcatctga
    15001 aatttccgta ccgaattcat cttcaagctc taaaacgaat tccataatac taattgaatc
    15061 agcttttaaa tcatctttga tgttcaattc atccgtcact ttatcggtat ctatctcaaa
    15121 atgatttgaa ataattttcg ctactttgtt aaatacttct tcgcgagtca actttttcac
    15181 ctccaagaat catacacttg tcaattacct acaccatggc taattttact ggcatttttc
    15241 aggaatgtcc accttttttt caatggaacg gattgcttca gatatccaaa tactattctt
    15301 cgcttttttc aaattgttcg actaattgtc caactacatc tgtctcaagc attgtgtgga
    15361 tttggcggat cgtatagcga acagcatcgg ggcctgttgc tccatgagtt ttaatgactg
    15421 gtgctttcaa gccaaacaaa acggcaccgc catgtttgga ataatccatt tctgctttca
    15481 aagaacgtaa gccgtctttt aaaagcatag cgcccatttt tcctttgatc ccttcattta
    15541 gaatagcaga ttttaataga ttcatcatat tcattgctgt tccttcaata gatttcaaaa
    15601 cagcgttgcc tgtaaatcca tctgtgacca ctacatcagc cacaccattg agtagctcac
    15661 gagcttctac gtttccaata aaattcaagc ttgtttcatt ctgaaggagt tcataggctt
    15721 ttttcgtcac ttcactacct tttgttgctt ctgttccgtt gtttaagagt gcgacacgag
    15781 gacgtccgat acctcggact ttacgtgcat aaaaagaacc taaaataccg tattgcaaca
    15841 aatgttctgg tttgttttct gcgttagcgc caagatccag catatcgaaa ccggcacctt
    15901 ctttccctac tactggcaag gtagacatca agccagggcg ttcgatattt ttgatacgtc
    15961 ctacgatgaa caaccctgct gctagtagcg cgcctgtatt ccctgctgaa aatacagcat
    16021 ctgcttcacc gtttttcact gcttgtgcag caaggaccat tgatgcagtc ttttttcgtc
    16081 gaattgcttt tactggctca tcatcactat tgattttttc gtctgtatgg ataatcgtga
    16141 tgtttttttc atttgttaag tatttcttga tttctgcttc ttttccgtaa agtaagaatt
    16201 ctacttctgg aaaatcttgc tgcgctaaag ccacgccttc tacaattgct tgaggtgcgt
    16261 gatctccgcc cattgcatct acggcaattt tcattgtttc cactcctttt tccgtcatca
    16321 gtataaagta tagcacagtc tgctaatcga aataacgatt atcctgattt tctatttgga
    16381 ggctttgcgc taaaggctga tactcaggaa gcatctgcca atcctttatc tgccaaaggg
    16441 ctgcagcctc ttctctggct acctccaaaa catttccatc cgtcaccaag tcagcaatcg
    16501 caaattgagg cagaccagat tgacggaagc caaatacttc accaggacca cgtaattcca
    16561 gatctttttc gcttagtaca aaaccattgg tggtctctgt catgattttc atccgttcca
    16621 cacctagttc atttttagga ttagcaacta agatacagta ggaagcatca cttccgcgtc
    16681 caacacgtcc acgaagttga tgcagctgtg ccaatccaaa tcgatccgca tccatgatca
    16741 gcatgacggt cgcgttcggc acgtttaccc ctacttcaat tacagtagtc gaaactaata
    16801 tctgcatctg gttgtctttg aaggcttcca tgattacttc tttttcctga ttcttcattt
    16861 taccatgtaa caagccaact tcatattttg gtgcaaatag ttcacgaagt ttttcataga
    16921 tttccactgc attttttaca tctaatgctt ctgattcttc aatcagcgga cagatgacat
    16981 acatttgatg tcctcttgcc agttcttttt tcgtccaatc cagtacactg tccaattgag
    17041 gtgttcttac ccagcgtgtt tcgatcggga tacgtccagc tggaagttca tcaatgatag
    17101 atacatccat ctcaccatat gctgtaatag ctaatgttct aggaatcgga gtcgctgtca
    17161 taaataagac atctggtcgc cagccttttt cacgaagtac tttccgctgg ttcacgccaa
    17221 aacggtgctg ctcatccgta ataactagac ctaaattctg aaattcgact ccatcttgga
    17281 tcaatgcatg tgtgccgatc acgatgttaa tttccccttg ctctagtttt tcaaggagct
    17341 cacgacgttc ttttgctttc gttgaccccg taagcaatgc gatggttact tcattgggat
    17401 caaacagctg ttgcaagctt tccatatgtt gttgtgccaa gatttctgta ggcaccatca
    17461 acgcaccttg gaaacctgct gtaacggtag cgtataacgc aatcgcggca accactgttt
    17521 ttccgctacc tacatctccc tgcaaaagtc gctgcatatg ctgcggactg cgaagatccc
    17581 gacagatttc attcgttaca cgtttttgtg catttgtcag ttcaaaaggt agcttttgag
    17641 taaattcttt caatttttgg acatcataaa ggatctccaa accattcgtt tctgcttttt
    17701 ctgctttttt caatccttga agacgcattt gaaataaaaa gaactcttca aaaacgacac
    17761 gtcgtttggc ttgatggttt tcttcgtggt cttttggaaa atgcatactt cttacagcag
    17821 aagctcggtc aagcagtcga tatttctcca caaggttgtt tggcagattt tcttcaatca
    17881 atgtgccgta ttcttgaaac ccttttttga ttagatcaac taatgtcgtt tgacgaattg
    17941 atttgttgac gtgatatatc ggcgaaaagt catccattgt ttgagaacct aaaatcttca
    18001 tcccgttcaa tgctttacgc tttgcatccc atttaccgta tacggcgatt tcctccgata
    18061 aaatgacttt ttcttttaga taaggttgat taaaaaaaga aacgttgaac acatcatgtt
    18121 cttgcatcat acgaaattgc aatcggcttt ttttataacc aaatcgattc atcacaggag
    18181 gcgagactac gattcctttc aaagtaacct tttcttgatc ttggatctcg tttagatttc
    18241 tttcctgaat atcttcgtag cggaaaggat aataagtcag taagtcttca atcgtttgga
    18301 tgcccaagct ggcaagacta gctgctcttt tttctccgac tcctgccagc atagtgattg
    18361 gatcatctaa agaacgcata aatttttcct ccctccctta acaaaaaaag gacttggtca
    18421 gcgatcatac ctgatcacta accgaaggcc ttttatgaat gaatactgtg ttagaaagat
    18481 attctgacac agaaattatt cagcagaaaa tagataagga tagacaggct gatctccttg
    18541 gtggatctcg atttccaaat caggatgttt ttctgttagt ttctcagcaa attcatttgc
    18601 ttgttcaact gttccttcat cacctgccaa gatcgttacg atctctgtgt cctcattgat
    18661 catttgttct agtgtactta acgctgttgc tgtgatatct gggttagaga tcaggatttt
    18721 tccatcgatc atgcccaaaa agtctccttc tttgatctct acaccgtcaa ttgaggtatc
    18781 tctgatcgcg tgagtgactt gaccgctcac aacgctagac aacatttctg tcatcgtttc
    18841 tttgttttct tctaaagaag cttgatcatt gaatgaaagc atagctgtca atccttggga
    18901 aatcgttctt gaaggaacaa ctgctactgg gatatctgct acttctgctg cttgatcagc
    18961 tgccatgaag atgttcttat tgtttggcaa gacaatcact ttatctgcgt ttacttcttg
    19021 aatcgcttta acaatgtctt ccgtacttgg attcattgtt tgcccaccac tgataacata
    19081 attagcgcct aaactttcaa acagctcttg gacgccttta ccagcagcga tcgctacgat
    19141 accatacggg atacgtggtg tttctttttt cgcaaactcg ttcacttctt catcgtgttc
    19201 caaaatcgtc tcatgctgca agcgcatgtt gtcgactttg attttgacta atgaaccgaa
    19261 tttttgtcca taattcaaga cttctcctgg gttttctgta tgcacgtgga ctttgatgat
    19321 ctcgtcatca ttgaccacaa gtaaagaatc tcctatacca tctaaatagt tacggaattc
    19381 ttcgtaatcg aatgtactat cgactgtagg tccttctccg atttttacca tgatttctgt
    19441 acagtaacca aaatggatat cttctgtcgc catttgacct tggacactgc gatgatgttc
    19501 tgcattgacc atatcatcca tttctgctgg agaaggttca tatgtttcat ccgcttggaa
    19561 ttctccattc aatgcactta aaaagccctc ataaacaaac agcagtcctt gccctccact
    19621 gtctacgaca ccgacttctt ttaggactgg aagcaagtct ggagttttag ctaaagcacg
    19681 ctttgcccct tttacaacag ctgtcattac ttcaacgaca tcatcagtac tttttgcttt
    19741 cttttctcca gctttcgctg cttctctagc tactgtcaaa atcgttcctt ctactggttt
    19801 cattactgct ttatacgcag tttcaactcc atgagtgaag gctgcagcaa gatcggaagc
    19861 atttaatgag acaacatctg gaatttgctt agaaaatcca cgaaacaatt gagacaaaat
    19921 aacgccggaa tttccgcgcg cacccatcag caatcctttt gaaagcattg ctgctaattc
    19981 tcctactttt tctgaggttg aatctactac tgccttagct ccactagtca tagatagatt
    20041 catatttgtt cccgtatcgc catctggcac agggaataca tttaatgaat tgacgtattc
    20101 tgcatttact tgcagacgat tcgcacctgc ctggaccatt tcctggaact ggcttgcact
    20161 tatttctgtt acactcacct agataatcct ccttcagatc gactgctgag ttaccagcca
    20221 gtctcctgcc tagtctggca gtacacgaac accttgtaca aagacattaa ctgaattggc
    20281 agttacgcct aataatgcct ctagattgta tttcactttt tcttggacgt tgcgggaaac
    20341 ttcagagatt ttcgtaccat agctgataat cgtataaaca tctacagcaa taccattatc
    20401 ttcttggcgg acaactactc ctcttgagta attttcttta cgcaaaatct cagtaatatt
    20461 atctttaatt tgatttttgc tggccattcc tacaacaccg aacacatctg tcgcggcgcc
    20521 accaacaact gttgcaatta catcattggt aatttcaatg gttccagctg acgtttgaat
    20581 ttttacagcc atgaatgagc ctccttaacg ggtaaaaaac tcccattttt cttctatcat
    20641 attttatcat agcgcaactt gaaataaaag gcagttaccc aaagtactca aaaaccttgc
    20701 aaaatttcag gtataacctt gtttaattta caattattct tctcaattgt caattgtatc
    20761 tatgaaaatg tacttgcaaa tttccattct atatgatatg ttagtctggt atgagctgaa
    20821 ttattctatt gctccaaatc aaggcaaagg aggaaattac catggcaaaa gtttgttact
    20881 ttacaggtcg caagacaaaa agtggaaaca accgttcaca cgctatgaac tcaacaaaac
    20941 gtactgttaa acctaactta caaaaagttc gcgttatggt cgacggaaaa cctaaaaaag
    21001 tttgggtgtc aactcgtgct ttgaaatcag gaaaagttga acgcgtttaa ttgaatgata
    21061 cttggaccgt tcagatagtg aacggttttt tatttaataa aaatttttaa gggtgggact
    21121 tttgattcct accctttttt tgcgtttatt tttgttatac gaacaaatta tgaagttatt
    21181 tattctaaat tttatttttt tgtgaaaatt agactctttt taagcagaga aaaacaagga
    21241 tatgataaac tgaatacaac ttcatagaaa tttggtggaa aataaatatg aaaaaaaatt
    21301 taaagtcatt ctttagagaa aacggacgct acgcagcagc tagtttcctg attccgtttt
    21361 tgattttagc acttgtttat ctaagtatcg ggatctatcc tggaagcagc agaagtattt
    21421 tggcaagtga tgccttttca cagttttcta attttcatgc cagcttccga aatgttcttc
    21481 ttggaaagca aagtattttt tatacatgga atgcttctct aggattgaat tatttatctt
    21541 taatttctta ctatctaggc gggatcttca caccactggt gttctttttt ccaaaccaga
    21601 tgatgccaga tgcactgtat gttctgactt tgcttaaggt cggctgtgcc ggattggctt
    21661 tttggtttct tgcacgtacg ttcaagattc ctcgctgggg gcatgttgca cttagcgtca
    21721 gttacgcatt gatctcattt atcactgcac attcggagat catcatgtgg ctggatgcat
    21781 ttgtttatct tccattagtg attttgggga ttcatcgtgt tatggatctt aaacggccga
    21841 aacttttatt tatcagttat ttactcttgt ttttatccag tttttatatg ggttttatga
    21901 ttggcgtatt ttctttctta tattttatcg cccgtctatt gacaaactgg tcagaatata
    21961 aaaaaacgat tgttccttat ggtatcactt ctttactggc aggcggcgct tcaatgatca
    22021 ttgtattgcc agcagtgctg gatcttcggt caaatggcga ggcactgagt cagatcacta
    22081 cgttgaaaac agaagcgact tcttatctgg atctaatcat gaaaaatatg attggtgttt
    22141 atgacacgac aaaatacgga tcgattccgt tcgtttacgc aggtctgcta ccattagtcc
    22201 tctgtctgtt ttattttgcc agtcggaaag taccgttgaa aaataaattt ttgtacggca
    22261 gtcttttcgc catacttatc gcaagtttct atcttgttcc attgaatttg ttctggcatg
    22321 gtatgcatgc accgaatatg tttttattcc gttatgccta tctgttctct ttcttagtgc
    22381 tcctgcttgc aggaaaagga tgggaaaaga tagaacaaga cgatcgtggg ctgttgttag
    22441 gaatcatttt ggtcgtagct gctctctttg cggtggcatg gggcatcaaa cctgaaggaa
    22501 gctacaccta tgtgacgatg acttcctttg tattgacagt ggttttctta gggatttatg
    22561 cttttgctgt gggattttat cagcataaaa aactttcatt caatcatctg agtatattgt
    22621 tattattgat catgtctgct gaagcactgg tcaatacgaa cagtatgatc catggcattc
    22681 ttgatgattg gaattatgct tcccgcagtt tatatactga tccttatcct gacttgaaag
    22741 ctcttgtaaa tgaaacaaaa aaagattccg actcgtttta ccgcttggaa aatctggatc
    22801 cagtatctgc aaacgattcg atcaactatg gttacagtgg tatcagtttg ttttcttcta
    22861 taagaaatcg taattcttcc tcttacctgg ataaattagg tttccgttca agagggacaa
    22921 atttgaatat ccgttataac aataatactt tgctaatgga tgggtttaca ggaatcaaat
    22981 ataacatttc taaaaacgaa agcacgttca gtaaatatgg cttccgccaa gctggtcaaa
    23041 gtggcgacta tacactgtat cagaatgtca atgcattgcc actagcattt actgcaccgt
    23101 tgtccgttaa tgatgttgag cagccagtga cagataattt gaccagtcag acaaacttga
    23161 tcaatacatt gtctggttta aatgaacagt attttacatt ctatacacct actttgaaga
    23221 atcagttgaa tacgacgatc acaaagacaa cgactggcgt cacttactca gaaacagcag
    23281 ataaccagcc aaaagagctc acttgggaag tccatgtgcc tgcaaataca caagcttact
    23341 taagtttgtt cccaaccaat tttgcacaat tagaaagttc tagtgcgacg atcactgtca
    23401 atggtacatc gcgaaaaacg caaatcaaca tttctggtca atactatgat cttggctatt
    23461 atcctcaaga gacaacagtg acatttactg caagttttta tggaacgaaa gaagtaagtt
    23521 tcatggagcc taaagtcgtt ggtctagatg ttgtagccta tcaagccgca atgaatcaga
    23581 tcaaagaaaa aggtgtcgaa atggaagcca ctggcagaaa agcttctggg actgtaaata
    23641 ccgctgaaga ttccatgttg gtgacaacta ttccttatga caaaggatgg aaagctaaaa
    23701 ttgatgggaa accagtcaag gtcagcaatt tcaaagacgc attcttgatg ataaaagttc
    23761 cagcaggtaa acatactgtc actttcagtt acttgcctga aggatttacg ataggtgcaa
    23821 tcctttttgt cctatgtatt gcattgttta ttctttatgt ctggtacctt gaccgcaatc
    23881 gcccctttta tggcacgatt gatccagcaa tagaagaaaa accgcgaaat acacacagaa
    23941 gaagacgaag aaaataaaga aacgatggaa tctgtgtcac tcttctattt aaatggcaaa
    24001 aatttcaatc tttaaatgac aaaaaccacc tgatttttgg tggtttttta tatacctatg
    24061 actaaactat ctcttgctgc ttcgacaact tctacagtga tcttatccat ttgattgatt
    24121 cttaaaatcc gatcaatctg cgcaaataaa cgatgaataa gcctaaaatt tccttttgtg
    24181 atcttaatga tagttgtgat agcctcataa tcagaaaaat cttctagctt gatatcaatt
    24241 cctaaatctg cccatttgta ttccaaaata tgatgtgttt cgtccttact aaggttatca
    24301 aattcatgtg caaatcctat gcgagagtaa agctgcggat aacgagacaa tcttttttca
    24361 ataccaggca taccgataag aaccattgcc agatcatttc tatcgtaaat atctcttagc
    24421 tgttctaaat gctgaagctt taaacgatca atttcatcga caataattaa gtcaattgct
    24481 tcataggttt taggcacagg aggatcttct tgattgtatt ttagtaccat atgcattgct
    24541 ttagtcagac ctattctact agttattgtg ttaatatccg aactgacacg agacatcttt
    24601 tcagctggta cagtataaaa aactttattt acacttaata tttcatctgt tgcatttatc
    24661 aagaacaaga aaaagtctga atattcaaaa aataacggtg tacattttta aaaggagatg
    24721 tataatcgag atttagaaag cgttttccta aaaatttaat gaattaggaa gtgattacta
    24781 tgaaaaaatt atttagcgca ctttttattc tgttatttgg ctttattggt attgggactt
    24841 ctggaaatat agttaaagct agcacgatgg ttgaacaaac aagtgaaatg gcaaaaactt
    24901 ttagtcaatt aacaccagaa gaacaaagtt acttcaaatc caaaggattt agtgataaag
    24961 atacttttta cactcaaaag atagagcctc aagatccgaa aacacgtttt tctgcaaatg
    25021 tattgcggtt aaccgcatct actaaaaaaa ttactaatac gcaggggcgg acagaatata
    25081 ttataacaag tcccaattcg gcgatgtatc gtatttctac acgcttgagt ttaggtagtg
    25141 caaaatcata tactcaaaca gtaaatctag ctaattctta aggttatggt ggaggaatgt
    25201 attttacatt tactgggaaa agacaatatt acagtgtaaa attagctatg caagtttata
    25261 atacatttgg tgtagggtca gtctcttgca ctgcgggagg attgacaata ggaaaatgat
    25321 aatttcttac tatgtcttaa atagaagtga tgatgaagga ctcttcgttt ttcaatcatt
    25381 acatgaggat acgatgctaa aagagtttta taatcggatt aagaataaac agatagaaac
    25441 aaatttatat tttttaagtg actctatctt gccttttatt agcaacccta aaattcttaa
    25501 cttgttgcag acaaaagatt tgaaaagttt accgattatt gtcattgatg aggaaattgt
    25561 aaaatatggc gcttatctaa gcgtagaaga attagaaaaa ttaactaaca ttggaatgag
    25621 tattcaggag gataatgggg tacatggaac atagaatgaa tatgcacagt tgagaacgga
    25681 atttgacatt tatttagacc aacagggtat accctgttgg tctttttgtg cgttcatgat
    25741 atacttggtg tcatttttat atttaaatgg cacaaatatt tctaattatt tgtcatttaa
    25801 cgctgaaaat ttttgctatt tatctaaaaa agtgacagga tactcatttg gccttcatag
    25861 aattttctaa atatgaatca tcaatgaatt taattaaaaa aataagtagg gcaaaatata
    25921 aatgtttttg ccttacttgt ttcc
//
LOCUS       DACKAC010000040        25361 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.040, whole genome shotgun sequence.
ACCESSION   DACKAC010000040 DACKAC010000000
VERSION     DACKAC010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.040"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(135..512)
                     /locus_tag="I0B46_001682"
     CDS             complement(135..512)
                     /locus_tag="I0B46_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2083986.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            768..1496
                     /locus_tag="I0B46_001683"
     CDS             768..1496
                     /locus_tag="I0B46_001683"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAR2083987.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1496..1750
                     /gene="purS"
                     /locus_tag="I0B46_001684"
     CDS             1496..1750
                     /gene="purS"
                     /locus_tag="I0B46_001684"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAR2083988.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1752..2423
                     /gene="purQ"
                     /locus_tag="I0B46_001685"
     CDS             1752..2423
                     /gene="purQ"
                     /locus_tag="I0B46_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAR2083989.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2424..4646
                     /gene="purL"
                     /locus_tag="I0B46_001686"
     CDS             2424..4646
                     /gene="purL"
                     /locus_tag="I0B46_001686"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAR2083990.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4631..6070
                     /locus_tag="I0B46_001687"
     CDS             4631..6070
                     /locus_tag="I0B46_001687"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAR2083991.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6102..7145
                     /locus_tag="I0B46_001688"
     CDS             6102..7145
                     /locus_tag="I0B46_001688"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAR2083992.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            7142..7720
                     /gene="purN"
                     /locus_tag="I0B46_001689"
     CDS             7142..7720
                     /gene="purN"
                     /locus_tag="I0B46_001689"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAR2083993.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7717..9252
                     /gene="purH"
                     /locus_tag="I0B46_001690"
     CDS             7717..9252
                     /gene="purH"
                     /locus_tag="I0B46_001690"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAR2083994.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9268..10542
                     /gene="purD"
                     /locus_tag="I0B46_001691"
     CDS             9268..10542
                     /gene="purD"
                     /locus_tag="I0B46_001691"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAR2083995.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10664..11578
                     /locus_tag="I0B46_001692"
     CDS             10664..11578
                     /locus_tag="I0B46_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAR2083996.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11711..13276)
                     /locus_tag="I0B46_001693"
     CDS             complement(11711..13276)
                     /locus_tag="I0B46_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAR2083997.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13269..14720)
                     /locus_tag="I0B46_001694"
     CDS             complement(13269..14720)
                     /locus_tag="I0B46_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAR2083998.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14734..15750)
                     /locus_tag="I0B46_001695"
     CDS             complement(14734..15750)
                     /locus_tag="I0B46_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAR2083999.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15970..17058
                     /locus_tag="I0B46_001696"
     CDS             15970..17058
                     /locus_tag="I0B46_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAR2084000.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17112..18638
                     /locus_tag="I0B46_001697"
     CDS             17112..18638
                     /locus_tag="I0B46_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAR2084001.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18640..19845
                     /locus_tag="I0B46_001698"
     CDS             18640..19845
                     /locus_tag="I0B46_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAR2084002.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20102..21079
                     /gene="guaC"
                     /locus_tag="I0B46_001699"
     CDS             20102..21079
                     /gene="guaC"
                     /locus_tag="I0B46_001699"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAR2084003.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21343..22767
                     /locus_tag="I0B46_001700"
     CDS             21343..22767
                     /locus_tag="I0B46_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAR2084004.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            23228..24337
                     /gene="pdhA"
                     /locus_tag="I0B46_001701"
     CDS             23228..24337
                     /gene="pdhA"
                     /locus_tag="I0B46_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAR2084005.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            24340..25317
                     /locus_tag="I0B46_001702"
     CDS             24340..25317
                     /locus_tag="I0B46_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAR2084006.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT         8204 a         4317 c         5696 g         7144 t
ORIGIN      
        1 aaccaaaaaa gaagcagatc cgttagcggt tctgcttctt ttagtttatt atattttctt
       61 atcaataata ggaacctgat tttaaaagcg gctacctctt taccgtgcct ctttacatac
      121 aaaaaataga ggaattaagc gtaaatggct tggctattat aactaaaatg ctggttatgg
      181 agatgttgca gcaagttttc gagaaaagcg tattccatct cttgtccatt gaataagatc
      241 tggatacgat aatgattttt gaaacgcttc tgttcatgaa tctgactgat tgaaagttcg
      301 cctttgtgtt ctgtctgata aagaaaagtc cttttacggc attcagcaca gcgcttggtt
      361 gctcggcgga tttttcggaa tgtataaaaa ctagtatctt tattgcataa ataaacgtct
      421 ttgatcgaca gcaaatcatt atatagacag gaacgttctt cagctgttaa ttctgataaa
      481 aaaagctgac cttgttttat tgcatcttcc atcggcaagt gcctcctcga acccattatc
      541 caaattttta gttaattcta atataccatc attaagtata aatgtaaacg cttgttgatg
      601 gtataagatt ttcgtttctg ataaaacacg aacattaaaa gaatctaatg ccttaaatgt
      661 acatattttt gttgatttat aaaaagatga ttgctaaaat ttaaagtgaa aaaacgaaca
      721 aaaaacgatg tatttgcttt taatgtaaat aaaaaggaga gacgtacatg gagaagaaag
      781 aacttttgta tgaaggaaaa gccaaaaaaa tatatcttac ttctgatgag aacttgattt
      841 ggatagaata tttagatcaa gcaactgcgt tgaatggcgc gaggaaagac cagataagtg
      901 gaaaaggaga attgaataat caaatcacaa gtgcaatttt ccgttatctg actcaaagag
      961 gaataaaaaa tcattttatt gaacaattgt ctaaaaccga acaattagca aaaaaagtac
     1021 aaattatccc tctggaagtg gttatccgaa atattgcagc aggaagtttc tcaaaacgtc
     1081 taggtgttcc cgaaggtaaa gaattcatca aaccaatcat tgaattctac tataaggatg
     1141 accagttgga tgatccattt atcaatgaag agcatatcta tttcttacaa cttgcaaagc
     1201 cggaagaaat cagtgagctg aaagcccaag cgttggctat taatgaaatt ttgaccgaat
     1261 tatttgctaa ggtcggtttg gttttagtag atttcaagct agaatttggc cggtcgaatg
     1321 gacaaatttt gcttgcggat gaagtaacac cggatacttg ccgattatgg gatgaacgta
     1381 cgaatgaaca tatggacaaa gacgtttacc gcagagatct aggtgatttg atcccagtct
     1441 atcaagaagt atataaccgt ctatcggaac taactgaaga aaaggagcga gtatgatgta
     1501 tttcgtaaaa gtatatgtaa cctataaaga ttcagtgtta gatcctcaag gggaggcagt
     1561 aaaaaaagca gtccatcgta tgggatatga aacaatcgaa gatatccgaa taggaaaata
     1621 ctttgagata caacttgctg atgcagaaga accaatcgat acagtgattg aaacgatttg
     1681 tgacaagctg cttgcaaatg taaatatgga aacataccga tatgaaatca aaaaaatgga
     1741 ggaagtataa catgaaattt gcggtcatcg tttttccagg ttcaaattgt gacgcagata
     1801 tgttatgggc agttcgcgag atcatgggcg ctgatgccga atttgtacgg catgatgagt
     1861 cctctttaga aggatttgat ggggttttac tgccgggagg attctcctat ggtgactatt
     1921 tgagatgcgg tgcgattgct cggttttcta caatcatgga agaagtgatt cgttttgctg
     1981 aagaagggaa accagtattc ggtacatgca atggattcca gatattgaca gaagcaggac
     2041 ttttgccagg tgtactgaga cgaaatgagt ctttgcattt tatttgcaaa actgtaccat
     2101 tgaaagtcaa ccccaagacg gcctttactt cagagtatga agccgacgaa ataatctatc
     2161 ttccagttgc tcatggggaa ggaaattatt tctgtgacga agaaacactt caagaactaa
     2221 aaaaaaataa tcagattgcc tttacttata atgacaacat caacggcagc gtggaaaaca
     2281 ttgcagggat tacgaacaaa gcagggaatg tgttaggaat gatgcctcat ccagaacgtg
     2341 cagtagaaga cttgctagga tcagaagatg ggaaacggtt ctttgcttcc attttaaaaa
     2401 acttcggaaa ggtgaccgta tagatgaaaa cattgaaaga accgacagct gaagagatca
     2461 agaaaaaaag aatctatgcc gaatggggat tgactgatga agaatatcga atgatcgaag
     2521 aagatatctt aggaagaatg cctaattata cggaaactgg actgttttct gttatgtgga
     2581 gcgaacattg ttcctataaa aattctaaac cggttctacg caaatttcct actagtggac
     2641 ctcatgtgtt gcaaggccct ggagaaggcg caggcatcgt cgacatcggt gatggactag
     2701 cagttgtctt caaagcagaa agtcacaatc atccttcagc cgtggagcct tatgaaggag
     2761 ctgcgacagg agttggcgga atcatcagag atattttcag catgggggct cgtccaattg
     2821 cgttgctaga ttcattgagg ttcggcgaat tagataatcc aagaacgaaa tatttactag
     2881 aagaagtagt tgcaggtatc ggcggatacg gcaattgtat cggcataccg actgtaggcg
     2941 gcgaaattgc atttgatcct tgttatgaag gaaacccatt agtcaatgca atgtgcgtag
     3001 gtttgattga acacaaacat atccagaaag ggcaagccgc tggcgtcggt aattcgatca
     3061 tgtatgtagg tgcaaaaaca ggtcgcgacg gtatccacgg agcaactttt gcctcagaag
     3121 aatttaatca agaagaagaa cagcaacgtt ctgctgtaca agtaggcgat ccttttatgg
     3181 aaaagctgct gcttgaagcg tgtttggaat taatcttgca tcattcggat tctctaatag
     3241 gtatacagga tatgggagca gcaggacttg tttcatctag tgcggaaatg gcttcaaaag
     3301 ctggctcagg acttatattg acattagatg aagtcccaca aagagaaact gggatgacac
     3361 cttatgaaat gatgttatcg gaatcacaag aacggatgtt gatttgtgtt aaatcaggag
     3421 aggaagaaaa aatccaagaa ctatttcaga agtatgatct agatgcagtc actatcggta
     3481 aagtaacaga cgacgggcaa tatcggctat atcatcaagg agaagaagta gcaaatctgc
     3541 cagtcgatgc attagctgaa gatgcaccag tttatcataa agaaatgaaa gaaccagctc
     3601 gaatcgtaga atttcagcag ttagctcctt atcagccagt tatcgaagaa cctggacaag
     3661 tattgcttga tctgctgcag caaccgacga ttgcttcaaa acgttcaatc tacgaaactt
     3721 atgattcaca agtacaaacc aatacagttg tccgtccagg aagcgatgct gctgtgatga
     3781 gggttagagg gacgaataaa gcgttagcta tgaccacaga ctgcaacgcg cgctatctgt
     3841 atttggatcc aaaaatcgga ggacaaatag cagtggcaga agctgctaga aatattattg
     3901 ctagcggtgg taagcccttg gcgatcaccg actgcctgaa ctatggttct cctgataaac
     3961 cagaagtttt ttgggaatta tcgacttcag ctgacggaat tgctgctgcg tgtgaaacgt
     4021 taggaactcc tgttatttca ggaaatgtct ctttatataa tgaaacggat ggtcaagcaa
     4081 tctatccaac accaatgatt ggtatggtcg gattgattga ggatcataaa catatcacta
     4141 ctcaggaatt caaaaaatca ggggatctaa tctatatatt aggtaagacg tttgcagatt
     4201 ttgatggcag tgaattacaa aagatgcagc ttggaagaat cgaaggtgtg atccgaaact
     4261 ttgatctatc cattgaaaag agaaaccaag agttagtatt gacagccatc caaaacggat
     4321 tgattgaaag tgctcatgac tgttcagaag gtggcttggc aattgcactg gcagaatcag
     4381 ctttcaaaca tcagctaggg atatctgtac agtttgaatt gtcctctgct caattatttg
     4441 cagaaactca atcaagattt gtgttgacag tcgcaccgga aaataagaca cgattcgaag
     4501 aaatgatggg agatgctgct gttttagcag gtaaggtgac ggatgaagcg ataatcgaaa
     4561 tttcagcaac tgacggtcag atcaaaatag aaactgcagt agcaagaaag tgctgggagg
     4621 atgcaatcgt atgtcttatg aagtgaagag tttaaatgaa gaatgcggga tcttcggtgt
     4681 ttggggtcat ccagacgccg ctagtgtggc atacttcggg ctccatagtc ttcagcatcg
     4741 aggacaagaa ggtgcaggta tcgtgtccaa tgatcgagga aagcttaagg ggcatcgaga
     4801 tttggggctg ctttctgagg tattcaaaga tcaaagaaaa ttgcagcagt taacaggaga
     4861 agctgcaatc ggacatgtac gttatgcaac agcaggcaat ggaagtgtgg acaatattca
     4921 gccttttcta ttcaaatttc atgatcagtc gatcggctta gcgcataatg gtaacttgat
     4981 caatgctcgt tcccttagaa aagagttaga ggaaaatggg gcgatcttcc attctaactc
     5041 cgatacggag atattgatgc atttgatccg acgaagtgaa gaaacagatt ttttaaataa
     5101 attaaaaagt gctttgaatg aagtcaaagg tggctttgcc tatttattaa tgacagaaac
     5161 ggcaatgatt gccgctttgg acccaaatgg ttttcgtcca ttagcaatcg gacaaatgaa
     5221 aaacggagca tacgtgattg cctcggaaac ttgtgcatta gaagttattg gggcacgttt
     5281 tataagagat gtccagcctg gtgagatagt gatcatcaat gacgaaggaa tccagattga
     5341 ttgctttacg aaggacacgc agctttctat ttgttcgatg gaatatatct attttgccag
     5401 accagattca aacatcgcag ggatcaatgt gcatactgca cggaaaaata tgggacggaa
     5461 tttagcgaaa gaatcgccag taaaagcaga tatggtcgtt ggcgtaccta attcttcttt
     5521 gtcagccgca agcggttatg cggaagctag tggaatccct tatgaaatcg gattagtaaa
     5581 aaatcaatac attgcccgaa catttatcca gccaacgcct gagctaagag agcaaggtgt
     5641 tcgaatgaaa ttatctgctg tacgaggagt agtggaaggt aaaaaggtga ttatggtcga
     5701 tgattcaatt gttcggggta cgacaagcag acggatcgtc catcttttga aagaagcagg
     5761 ggcaaaagaa gtccatgtga ggattgcttc tcctcctttg aaatatccat gtttttacgg
     5821 gattgatatt caaacgcgta aagaactcat tgcggcaaat cacacgattg atgaaatcaa
     5881 agaatgtatc ggtgcagatt ctttaggttt tttaagtgaa gagggtctga tcgaatcgat
     5941 cggtctggaa aaagatgctc cgtattcagg attgtgtatg gcttatttca acggagatta
     6001 tccgacgccg ttatatgact atgaagaaaa atatcaggaa tcactaaaag aaaaagtatc
     6061 gttcttttaa aagcagacca agaagggagt atatgaattc aatggcaaat gcatacgcaa
     6121 aagctggcgt agatgtcgaa gctggatatg aagtagtaga acggatcaag aagcatgtca
     6181 aaaaaacgga acgtttaggt atgatgggcg ctataggagg attcggcggt tgttttgatt
     6241 tatcggtctt tcaattaaaa gagccagtcc ttgtctcggg gaccgatggc gtggggacga
     6301 aattagcagt ggcaatcgaa gcagacaaac acgatacaat cgggatcgac tgcgtagcga
     6361 tgtgtgtcaa tgatatctta gcacaaggcg cagagccgtt atatttctta gactacttag
     6421 caacggggaa aaatgatccg ttacgtttgg aaaatgtagt aaaaggtgta gcagatggat
     6481 gtgttcaagg aaatatggcg ctgattggcg gagaaacagc tgaaatgcct ggtatgtact
     6541 caggtaatga ttatgatcta gccggatttg ctgtagggat agctgaaaaa tctcaattat
     6601 tgacaggaga aacgatcaac ccaggagatg tgttgcttgg aatagcatct agcgggattc
     6661 attcaaatgg gtactctctt gttcgcaaaa ttttctttga gcagcagcag ttttctttat
     6721 catcaaaact gccagaattg aaaaaaacac ttggcgaaga attgcttgag ccaacgaaaa
     6781 tctatgtttc ttccttgctt ccgctgatca aagaaaagaa aataaaagga gctgcacata
     6841 tcactggtgg agggtttgta gaaaatatcc cgcgaatgtt accagaatct atggctgcac
     6901 aaatcaaact tggctcatgg ccgattcttc caatattcgg ctgtttacaa aaatatggtg
     6961 aaattcctga gatggagatg tatgaaatct tcaatatggg catcggtatg gtcctggcag
     7021 tcgaagaatc gaaagtaaaa gaaatagtac acgaaattga gcaagctggc gaaaaagttt
     7081 atgaaatcgg gaaagtgatc gccaaagaaa aacatcgtgt ggtgtttata ggagaagaac
     7141 gatgagaatc gctgtttttg cttctggaaa cggaagcaac tttcaagcgc tagccgatta
     7201 tctatcgaaa aaaggattgg aatcttcgat tgactggttg ttttgtgatc agcctgaagc
     7261 atatgttttg aaacgtgcaa ctgcgttgag tgtaccggca gattgttttt cacctaaaga
     7321 atttgattca aaaaaagagt atgaagaagc aattttgcat aaattgaaag aaaaaaagat
     7381 cgatttgatc gtattagctg gatatatgcg aatcattgga ccggtattgc tgaaaaatta
     7441 tgataaaaga atcatcaata tccatccgtc tcttttacct gccttccctg gtttgcatgg
     7501 catacgtgat gcctttgaag ctggagtaaa agaaacgggt gtcacaattc attatatcga
     7561 ccaaggtgta gatactggac caatcatccg gcaggaaaaa gtgagaattg aacaggagga
     7621 cacgttcgat tctttagaag aaaaaatcca tagagtagag catcgaatct atccagaagt
     7681 gattagtgaa attattgaaa atggaggcta tctgaaatga caagagcatt gatcagtgta
     7741 tcagataaaa ctgggattgt agcattcgcc caaggtttga ttgaacaagg agtagaaatc
     7801 atttcaacag gaggaacaaa gcatgcgttg gataaagctg ggatcaaaac gctttcgatc
     7861 gaagaagtga cagggtttcc tgaaatgatg gatggccgtg tcaaaacact tcatcctaaa
     7921 atccatggtg gtcttttagg aagacgcgat ctccctagcc atatggaggc aatggaaacg
     7981 gaaaatatcc aaccaatcga ctttgtttgt gtcaatttat atcctttcaa agaaacgatt
     8041 atgaaaccag atgtcgaaat agcagaagct attgaaaaca ttgatatcgg tggtcctagt
     8101 atgctccgta gtgcagcaaa aaaccatgaa tttgtcacct ctgttgttga tccatctgac
     8161 tatgaagaag tactttcaga attacgtcag tatgggcata cttcactttc tttcagacaa
     8221 aaactagcgg caaaagtctt tcgtcatact gcggcatatg atgcactgat cgctcaatac
     8281 ttaacggatt gggtggaaga aaaagaaccg gaaaaattga cacttacgta tgaacgaaaa
     8341 caaacgcttc gttacggtga aaatagccat caagccgctg ccttttatca gtccgctctt
     8401 ctggaaaaat attcgatcgc agcagcaaaa caactccatg ggaaagaact ttcttataat
     8461 aatatccgag atgcagatgc tgcgctacgg atcatgaagg aatatgacga gccaacggta
     8521 gtcgccttga aacacatgaa tccttgtggt attgggacag catctacgat tttagaagcg
     8581 tgggaagcag cgtatgaagc agatcctgtt tcaatctttg gtgggatcat cgttttgaat
     8641 cgagaagtag atctgccaac agcagaacag atgcataagc tgtttctaga aatcatcatt
     8701 gccccttctt atacacaaga agctttggaa ttactgcaaa aaaagaaaaa catccgatta
     8761 ctgacagtag actttgcgca ggaagaaaat caaaatcgag aagaaacggt ctcagtctta
     8821 ggtggacttc ttgtacaaga tcaagatctg gcaatagaaa atgaagaaga gtggaaagtc
     8881 gtgacaaaac gtaagccgac tccagcagaa gcaaaagcat tagcgtttgc ttggagagct
     8941 gtcaaacatg tgaaatctaa tgcgatcgta ctggcaaacg aacgtcagac agtaggaatc
     9001 ggagccgggc agatgaatcg agtcggttca gtaaaaattg cgattgaaca agcgcaagag
     9061 aaaatggaag gtgcagtttt agctagcgat gcttattttc caatggatga ttcggttgaa
     9121 tatgcagcca aacatggtat aaaagcaatc attcagccag gaggaagcat caaagaccaa
     9181 gattctattg atatggcgaa taaatacgat ctagcaatgg tctttactga tgttcggcat
     9241 tttagacatt agagaggagt cggataaatg aacattttgg tgatcggtgc aggtggtcgt
     9301 gaacatgcaa taagtaaaaa acttttagcc agtccgaagg tggatacggt gtattgtgcg
     9361 ccaggaaatc cggggatgac aaaagatggt atcaattgtg tcgctatcag tgagcgagat
     9421 cattcagcgt taatccattt tgttaagaaa gaggctattg attggagtat cgttggtcct
     9481 gaagttcctt tgttaaatgg gatcgtcgat gattttatgg cggaaggatt gaaaatcttc
     9541 ggtccgacaa aagcagcagc tctgatcgaa ggttcgaaaa gttttgctaa acaattaatg
     9601 gatacttatc aaatcccgac agctaaatcc agaagttttg aatgttacga aaaagcagca
     9661 gcgtatatca aagaaaatgg tgcgccaatc gtagtgaaag cggacggctt agcagctggg
     9721 aaaggtgtag tcgtggctac cacagtgaag gaagcgctgg atgcggcaga acaaatgttg
     9781 aaccagcatc gatttgggga tagtagcaaa aaaatcgtcg tagaagagtt tttggcagga
     9841 gaagaattct cccttttggc gtttgtccgt aataccaagg tgtatccgat ggtcatttcg
     9901 caagatcata agcgggcatt tgataacgat aaagggccaa atacaggcgg aatgggtgct
     9961 tattcaccag taccgcaaat ttcaaaaaag atgattgaaa aagctgtgga agaagtttta
    10021 aaaccagcgg ctttgggaat gaaagaacta ggacgctctt tcacgggtgt tttatatgcg
    10081 ggtttgattg ctacgaagga aggaccaaaa gtgatcgaat tcaatgcgcg tttcggtgat
    10141 cctgaaacgc aagtcgttct ttctcggttg aagagtgact ttgcgcaagt aatcgatgat
    10201 ttattagaga atcgtacagt agagttaaag tggcagcaag aaggatacaa tgtaggcgtt
    10261 gtggttgcag ctgccggtta ccccgaagaa tatgagacag gaatggttct accagatttt
    10321 ctggaagaag agctttctgt ttactatgct ggagtttcct caaataacgg caaactagtt
    10381 ggagcaggag gacgtcttta tttagtagaa gcatacggtg agacgatcaa agaagcacag
    10441 aaaaaaattt acaccgcgtt aatggataaa acgacagatg gaacatttta tcgtacagat
    10501 attggagaaa aagctgccaa tagcgaactg atcgtgaagt aaatcagctg cttccattta
    10561 taaaaaatac aacaaacata atcggaagcg ccgattttag tgaagccttt tcataaagag
    10621 tgtggtacac ttttgttgtg tatataaata aggaggagtt cagatgaaaa aaattcaatt
    10681 aggtacttct gatcagaaag cagctgcagt gatcttaggc tgtatgcgta tcaacaatgc
    10741 ggaagatcca gtaaaagtga ttgagacagc ttatgaaaac ggcattgatt tttttgacca
    10801 tgcagatatt tatggaaacg gtgaatgtga agaaatcttt gcagatgctc tagcaaaaac
    10861 atcaatcaaa cgagaagatc tttttattca aacaaaatgt ggaatcgttc caggtgtgat
    10921 gtttgatttt tctaaaaaac atatcattga atctgtagaa aaaagtttga cccgtctgaa
    10981 aatggattat gtggatgctt tacttcttca tcgtccagat acattggtag aaccagagga
    11041 agtagctgaa gcgtttgatg aattggaacg gtcaggaaaa gttcgttatt ttggcgtcag
    11101 caatcaaaaa ccaatgcaaa tcgaattgct gaaaaaatat gttcgtcaac cattagtagc
    11161 taatcagctg caatttggct tgaaacatac tggaatgatc gatcaaggga ttcatgtcaa
    11221 tatgacagat gaagcaagta tcgatcatga cggtagtgta cttgattatt cccgattgca
    11281 tgatatgacg atccaagcat ggtcaccata ccagtatgga ttttttgaag gtgtatttat
    11341 cgggaacgaa aaattccctg aattaaacca aacattagaa gaactagcag ctaaatatca
    11401 aacgacacca actggcttgg cttctgcttg gattttacgc catccagcca atatgcaagt
    11461 gatcgccggt acgatgaacg tagggcgaat caaggaaatc gctcaagctt cagaaattaa
    11521 tatgtctcgg gaagattggt acgaattgta ccgtgctgct ggaaacattt taccttaaag
    11581 attgtcataa gcaggtcaaa agggctataa attaaaaaga cagaataact tgaaagctgt
    11641 tttcagcttc gagatattct gtcttttttt attgcaaatg gaaagagtat cattgtacgg
    11701 atttcgttta ttagttattt ttgtttttaa ggaaaagctg tgtccgatat tcttttggag
    11761 aaatcccttg ttttttctta aataaataac tgaaatagtt agggtcatta taaccaattt
    11821 caaaagcaat atctgaaata cgatagtctg attgctgcaa taattttttt gctaattcga
    11881 ttctttgttc tgtcagataa tcgatgaatg tttggttaaa cgattgagaa aaaatcgtac
    11941 tgaaatgaga gggacttaat gcaacttgtt gagcgaccca agttaaggac atatctggat
    12001 cggtaaaatt ttctttgata aaatccaatg catggttcaa aattggctga tatttgttcc
    12061 ttgaaggctg tgagggcaga gcagttaaga aagaaatgaa catataaact gtcgaacggt
    12121 aaagcgagtc gtcggcgctg attgctaagc gttgactggt ggatgtgcaa tgaaccattt
    12181 cttgcgggat tttcttttct ttcaggcaga catgctggag tagcttagtc aagacaaata
    12241 aacgatataa tcgtgttcga gttggtgtgc cttgtggttt cgtcaagtgt tcgatgtact
    12301 tagatacttc gtttttcgat aatgaagaca aatcaagtga gaggtcaaag taaaagacag
    12361 aatcaactgg aagtttcgct tcttgaatca aatcctcata gctgataatt tgttctttaa
    12421 gggaactggc ggtttcaagt atcatctgcg tgatctcaaa agatgcaggt aattcgctga
    12481 ttctttctgc gacaggacca atggcgacag aaaaattatt tttttgtccc ttttctaatt
    12541 catggaccaa cgtttgggct acctgataac tggtctcgat cacattttct tcaagagaat
    12601 caactattaa aaatttgatg aatcgagagg aaacagcaga gaagatcact tgttgattct
    12661 ccctaaataa aaacttcaaa taatcactga gatgagcgta atcttcaaag tctgtactga
    12721 cttggtttgc tgctaataat acggtcattt ttttccctgc taaagagcga ttgaaagatt
    12781 cagcaagttt caatgtttca ctgatcgtaa gtgagccttt gaacaaaccg ttcaagaaat
    12841 gatttttttt caattcgaaa acaagatcag aagaagtgtg cgtttctttt tgctgcaaat
    12901 ctaaagcagt tactgctttt tctattgctt gtttcaattc gctactttta atcggtttaa
    12961 gcagataatc atctgcctgt acctggattg ctcctttcat ataatcgaaa tcgtcgaatc
    13021 cactaataaa tatgagacga gtccaaggaa gtagtttcct tgcttctttt gaaaaagtca
    13081 acccgtccat gaaaggcatt tggatatcag tcagaatgat gtcgggctgt aagtcgataa
    13141 ttgaagacag tgccatctca ccatctccag cttcgccgac ataagtgatt ggataataag
    13201 aaccaaggac agttatttgt tcccgcaagt tattgcgaat gaggtattcg tcttcgatga
    13261 taaaaacttt atacatgttt gtttcctcca attgttttgg gagatagagg gatcatcagt
    13321 gtgacagttg ttccttctcg gtatttgctg tcaatcgtga taggtttttg atcatttccg
    13381 taataaagaa ggagacgctt gtttacgttg taaagtccgt agccgatact gaaatctgaa
    13441 tcgatccctt tggctaactc tttacgtatt tctgccagtt tttctgcagg gatgccaatc
    13501 ccgttgtcta taactttgaa ttggatcatc tcatcaattt tgcatgtggt aatacgaata
    13561 aatcctttgt gacggacgta cttcgttcca tggtatacag cattttcaat caatggctgc
    13621 aagatcattt ttaaaataga aacaggaaaa agtgtttctg gtatatcgat cttatagtca
    13681 agcatatggc cgtatcttat ttttaagatg actagataat cattgatatg gctaatttct
    13741 ttttcaagag gaatccagtc ttgtcctttg cttaaagacg tacgaaaaaa gccggaaagt
    13801 gcatgggtca tagtcagagc ttcttcaaag cagtcttgtt cgatcaaaga aataatggca
    13861 tccaaactgt tataaataaa atggggagta atctgagctt gcaatacacg aacttcactt
    13921 tgtgctaaat ggtattgttt caaagcgttc tcccgtaaag actggtttag atcttccgcc
    13981 attttattca tgctagcggt aagtgtttgt aattccagtg tttccggcag ggttaaacga
    14041 aagccaaggt tccctgaaga aagttgttct gacatctcga tcagctgatt gatcggttct
    14101 tggacctttc tctctaaaaa gcgacgattc tttttcgcat agaaaaaaat ggtactaaga
    14161 actccaagtt ccactattgt taatagaatg agcgagcgga caagtgtcgt attttttttg
    14221 cttgctaagt cgatctccac acgaacaaaa tcttgtaaga tatctgacag cagttgagtg
    14281 acagaatcta cctgattgat gatctcttca ttggtatcca taaaatcttt tgccaagata
    14341 ttattgatca gctcgttttg ataatcttcc agtgtatcga tcgtacgtag cgatacatct
    14401 aacgtactaa tttctttaag tgtatttgta ttatttttga tctctcgaat atcattccta
    14461 agttcttcta ggatattttc ctcattgaac tcatctacgg ttacttggcc agaaatcaag
    14521 tcccacatac tttccaaaac tttttgatca accttcaaag caattgcatc tgctttttgg
    14581 atatttgcga tcgtctgttg ataaatcaaa agctgttgcg aatataacag tatgctcagt
    14641 agcaaaggga taacagcaaa tagaagcatg gctttgtttg cttgggcaaa aatctgtttt
    14701 atgcttaaat tttttgtcat gatacttctc cagtcaataa tttctggttg ggatgttagc
    14761 tatattctgc tgggaaaaga cagtctcgga aataaaaata tctttaggta atgtttttcc
    14821 tgtttgaata tttccaaaat aacgagtaac ggtattggct acataccagc cgacatcagg
    14881 tttacactcg actacacaat tgacgattcc tgagcgtagc ttctcgatca tatcttcttg
    14941 tccatcaata gttacaatga tcaaatcttc tccaggtttg attgatgttt cttttatcgc
    15001 agctaaagca ccgtgagtca tttcatcact atggctgtat aatacatcga tttcggataa
    15061 atcatttgtt tctaaatatc gtttcatctg gtcttttcct ttcatccgaa taaaatcacc
    15121 gacaattcgg tgaacaatag tcatcggagg tttatggtcg atggttccat ctttttttag
    15181 ggttggttta cgattgattg attccaaaaa tccttcggag cgtagcttgg tcggtgtgga
    15241 gttctctgaa ccagcaagtt ctaggattct gatttcctgt ttctttgaat ttgtgaagtg
    15301 attgctgaca aataaacccg cccgatttcc ttctgctttg aaactggggc caatgtgggt
    15361 gacgaaaagc tcgggatcat tcgtttgaat atgtctgtcg acaagtatga cagggatccc
    15421 tgctcgcttt gcttctttta atacagagtc ccacccggat tcttgcaaag gagtaaaaat
    15481 gatcatatcc actttatagg caatgaacgt ccgtatatct tggatttgcc gttcctgatt
    15541 catgtagcca tttcggtaga gaacttggta atcttctttt tcaagcgcag tgataattga
    15601 ttcagtatgc tttttccgcc agctactctc cgttcctgat tgcgaaaaac caacgactag
    15661 tgggtcaggt gcttcctttg tattaggtgt acaccccatc atttggtaag cgattacaac
    15721 aagggctaaa aaaaataaaa cacgtttcac tttaaatcac tccatctgga tttgttagta
    15781 ctatcttatc agataaaagg gaaaatcaaa gtcaaaagga atcaatcgaa aaaaatccaa
    15841 gaaacttaaa aaaatccaag acaaaaaaag tacacctaaa aaatttaaag taaatcccat
    15901 aaaaaaatca atgttaacgc tttctttatt agctatactc ggtgtatcaa ttgaaatggg
    15961 ggataaaaaa tgaaaaacaa atttagaaag attgttggga ctatcgctat tttgaccgca
    16021 agcattctat taagtgcgtg tggcaattca ggatctgcag atgatagtac ggggtatgta
    16081 ggaatcgcta tgccgaccaa atcagcagaa agatggatcg ctgatgggaa caacatggtc
    16141 tcggaattag aaaaactggg ttataaaaca gatcttcaat atggagaaga taaagtagaa
    16201 aatcaagtag cacaaataga aaatatgatt acaaaagggg tagatacact tgtgattgct
    16261 tctatcgatg gttcggcttt aactgatgtg ttagctaaag caaaagaagc agatatcaag
    16321 gtgatcgcct atgatcgttt actgatgaat tcagaaaatg tcgattatta cgcgactttt
    16381 gataattttg gagtaggtgt atcgcaagca gcttatattg aagagcactt aggattgaaa
    16441 gaaggaaaag gaccattcac gattgaatta tttggtggct cgcctgacga caacaacgca
    16501 ctaatcaatt acaacggagt gatgtctgtt ttacaaccat atatggacaa cggtcagttg
    16561 gtcgtgccat ctggacagac gagtttcagt caaatcgcta cgcttcgctg ggatggttct
    16621 acggcgcaag caaggatgga taatctgtta agcgcaaatt atactgacca aacattggat
    16681 gctgttttgt cgccatatga tccaataagt ttaggaatca tttcgtcatt aaaaggcgtg
    16741 gggtatggga gtgaaagtaa acctttacct gtgattactg gacaagatgc gacagttgct
    16801 ggcgttaaat cgatcattgc cggggaacaa acacaaacga tctttaaaga cacacgtatc
    16861 ttagcaaaaa atacgattga aatgatcaaa gcaatcagtg atggagaaga ggttccggtc
    16921 aatgatactg aaacttatga taatggggtg aaaactgtcc ctacgtattt agcaaataca
    16981 gtatctgtcg ataaagataa ttatcaagca gagctgatcg acaccgatta ttataaagaa
    17041 tccgatctga aaaattaaga ggaaccaaac aaggaactaa agagatctaa gtcagagaga
    17101 ggataagaat tatggcggat tgtattttaa aaatggcaga tatcaccaaa aaattttctg
    17161 gaatccgagc gttagattgc gtgaatctga tgattgaacg tggggaaatc catgcattgt
    17221 gcggagaaaa cggagcggga aaatcaacat tgatgaatgt cttgagcggc ttgtatccat
    17281 atggctccta tgacggggaa atcttttatg atggaaagct gtgtcaattt aaagatttga
    17341 aagatagtga agaaaaaggc atcgtcatca ttcatcaaga attagcacta agtccttatc
    17401 tttcaataaa agagaatatt tttctcggaa atgaacaagc agaaagaggc gtgattgatt
    17461 gggaactgac agaaaagaaa acatatgaac tgctgaataa agtcggtttg tctatcgacc
    17521 cgaatcttct agtatcccag attggcgtag ggcaacagca actagttgaa atagctaaag
    17581 cattatcaaa atccgtccgt ctgttgattt tggatgagcc taccgctgca ttgaatgaag
    17641 aagaaagcgc gaatttgctg agattgatcc gagagttgaa agagcaaggt gtaacctcca
    17701 tcattatttc acataagctg aatgaaatcg tggatatcgc agatcgaatc accattttac
    17761 gggatggaca gacgattgag acactagaaa atgaaatgat cagcgaagag catattattc
    17821 gcggaatggt gggcagagat ttagttgatc gttatcctga aagaaaaccg gatattggag
    17881 aagtttattt taaagtagaa gactggacgg tctatcaccc tcttgatcac gagcgaatca
    17941 taaacgacca catcaacttg catttgaaaa aaggagagat tgtaggaata gctggactga
    18001 tgggagcggg cagaactgaa ttcgctatga gtctatttgg acattcttat ggaagcaaga
    18061 tatccggacg tatattaaag gaaggggcag agatatcagt taagaccgtt ccttcagcca
    18121 ttgcaaacgg actcgcttat gtctcggagg accgaaaatc tttaggactg aatctgctga
    18181 tggatattcg ggaaaacgtg acgctgagca gtttggataa aataagtcaa aaaggagtat
    18241 tagataaaga aaaagaagta actgaggcag aaaatttccg gaaaaagatg cgtataaaaa
    18301 caaactccat ccatcaaatt gtcagtagtc ttagtggtgg gaatcaacaa aaagtcgttt
    18361 tatccaagtg gctgatgaca caacccgata ttcttttttt ggatgagcca acccgtggga
    18421 tagatgttgg ggcaaaatat gagatctaca cgattatcga agaaatggca gcaaatggca
    18481 aagctgtctg tcttatctct tctgaacttc ctgaaatatt agggatgtgt gatcgaatct
    18541 atacgatgaa cgaaggacgt attacaggag aagtctcgcg agaagatgca gatcaggaag
    18601 tcttgatgaa attaatgacg aaagaagagg tgggttaagt tggaacaggc aaaaaatctt
    18661 acggataaaa aagagaaaaa actaaatttg aaaagtagag cagctgatct ttttggtcgc
    18721 tacagcatgg tgatcatttt agcgggacta cttattgcgt ttcaattgat gacggatggt
    18781 attttctggc gtccattgaa tataactaac cttgttctgc aaaatagcca tatattagta
    18841 ctagctgcag ggatgttatt agttgtgctc ttaggtcatg tggatttgtc ggtagggtca
    18901 gtgatggcgt ttgttggtgc aattgcgggc atgatgatgg tcaatcgcca aatgaacccg
    18961 tggatgacag ttatcgcatg tcttgcagta ggcgggctga tcggcgcatg gcaaggtttt
    19021 tgggtagctt atgcagggat accggcgttt atcgtgactt tagcaggatt gctgatgttt
    19081 cgtggactga cacaagttgt tctaggaggc caatctctag caccttttcc agctgttttc
    19141 caaaaaatgt ctacgggtta tttaccagac ttaagcgaag gaagcagcct tcacttcttg
    19201 acattgattt taggaatgat catcacgatc gtgttagttg ttggtcaatg gcgaacaaga
    19261 aatcgtcgga aaaatcattt atttgaagtg gaatctccaa cagctttctt ggtaaaggcg
    19321 gtatttacag cagtcgttgt actaggaata acttatatct ttgcctctta tcaagggtat
    19381 ccgattattt tgatcattct tggtttgatc gttgcagcat atgggttttt gacaaataaa
    19441 acagtagcag gtcgacagat ttatgcgacg ggcggaaatc gaaaagcggc tgaactatca
    19501 gggatcaaaa caaagcgaat cacgttctgg gtattcgtca acatgggagt catggcagct
    19561 ttagctggat tagttttagc tgctcgattg aatgcggcaa cacctcaagc aggtacatcg
    19621 atggaactgg atgcaatggc ggcagtatat tttggtggtg cttcgacttc cggcggcatt
    19681 ggaacaatca tgggtgcgat tgtcggtggc ttggtcatgg gtgtattgaa caatggcatg
    19741 tcgattttag gcgtcggggt agactggcag caggcaatca aaggattgat cttgctttta
    19801 gccgttgtgc tagacttgta taataagaaa aaacgaacaa cgtaggagaa cgccggttat
    19861 tcagaaaaaa gactgtgaca agacatttgt cacagtcttt ttttcgctaa aagcgaacat
    19921 tcattgtttc gtttgtatag actaaaaata atatatgttt ttttaccatt cgtataaatt
    19981 ctctttttgc tcttgatttt tttgcttaat tgagttaaat tacatagggc gctaaaaaaa
    20041 gccttgatta aattaataat gttcgtgttt tgctggatgt gaacatcagg aggataatgt
    20101 aatgaaagta tttgattacg aagatattca attaattcct aataaatgta tcgtaaatag
    20161 ccgttctgaa tgtgatacaa cggtcacatt aggtaaacat acattcaaaa tgccagtagt
    20221 gcctgccaac atgcagacga ttattgatga aacaatcgca gaattcttag cggaaaatgg
    20281 ttatttctat atcatgcatc ggtttgatga agcagctcga attccgttta tcaaaaaaat
    20341 gaaaaagcgt ggattgatta cctcgatcag tgttggcgtc aagaaagagg aatattcctt
    20401 tatagagaag ttggcagaag aatcgctaaa tcctgattat atcactatcg acatcgcgca
    20461 tggacatgct aactcagtta ttgatatgat tcagcatatt aaaaaatatt tgcctgaaac
    20521 ttttgtgatt gccggtaatg ttggtacacc ggaagccgtc cgtgaattgg aaaatgcggg
    20581 cgcagatgca acaaaagtcg ggattggtcc aggaaaagta tgtattacaa aaatcaagac
    20641 aggctttggg actggaggct ggcaactggc agctcttaga tggtgtgcga aagcagctcg
    20701 caagccgatc atcgctgacg gtgggatcag aacacatggc gatattgcaa aatctgtccg
    20761 ttttggtgcg acaatggtca tgattggttc attgtttgcc ggtcacgagg aatctcctgg
    20821 cgaaacaaaa gtcgaaaatg gaatcgtgta taaagaatac tttggttctg cttcagaatt
    20881 ccaaaaaggc gaaaaacgaa atgttgaagg aaagaaaatc tggatccaac acaaaggatc
    20941 tttgaaagat acactagtgg aaatgcaaca agaccttcaa tcatctattt cttatgctgg
    21001 aggaagagat ctggaagcca ttcgaaaagt tgactatgtc atcgtgaaaa attcgatttt
    21061 taatggagac acaatctaaa aaataaataa aatattagga tgttgtgaaa aagttgtcct
    21121 gcattaagta ataagaacag ccaaacaaaa atagttcttt atatgttttg tttggctgga
    21181 attattatcg cagatacaag cttttgaaca tcattattca gaataaggga ctgtgataag
    21241 atttttgtca cagtccctta tgttttgcaa aaaaagtttt tcttttttct cgtaaattcg
    21301 gtaaaatcaa aagaaaaaat agacaataga aaagagaagc ttatgtggga aaaagtagag
    21361 aaaaaacaag gaccgattta tcgtcaaatt atggaacaga ttatgagaga aatcgaaaac
    21421 ggaaggatca atcctggaag tcaacttgaa tcagaacgaa aactagggca actttttggt
    21481 gtgaatcgtt ccaccgttgt ccatgcattg gaagagctta gggagcttgg ggtactaacg
    21541 agcaaacgtg gcagcggatg gtttgttaat cagactgaat gggggaaatt cgctgttcca
    21601 agaatcgatt ggcggcaaat gatctctcct cgttatgagc agacggactg gtatgagcaa
    21661 aaaataagag aagcaaagca gatccaaaaa acatcgtttc ttgatttata cgctagcgag
    21721 atgccagatg agcttttgcc gaattttgag tttccttctt attctcttga agaaatctta
    21781 gcggaagaaa aagaaatgga tcttttaggc tatactccgt tacgtaacaa aatcaaacgg
    21841 tatttggaaa gtaaatttga attggcattt tcccccaacc agcttcttgt gactggtggc
    21901 ggtcagcaag caatcttttt gattttacaa acgattcttt cagctggaga ggggatagct
    21961 gtagagtcac cttctttttt ttaccgatta cctcttttca aagcttcagg aacgcgttta
    22021 tttgggatac cgatggatga agaagggatc gacttagaaa aactgaaaga atcgatccat
    22081 aaaaataaat tgaaagcagt gcttatcaac ccgaattttc aaaaccctac aggaaaagtg
    22141 atgagtcaaa aacgacgaga aaagctagta gaattgtgtc gacagtatcg gcttccaatt
    22201 attgaagacg atgtatttac ggatctttca tttctaaacc aagaagaagt ccttcctata
    22261 cgaacgattg atccggataa tgtgttatac gtcggttcgt tatctagggt tttaggaaaa
    22321 acaacgaaaa tcggttgggt gataggacct gagactttta tctcgcaact agctcaagcg
    22381 caagaaatga tggagttttc gatgaatatc cttacacaaa tcacagtggc taatgtattt
    22441 aatgaagcaa ttgatttgaa aatgcaagat gtaagaaaaa agttgaaaga aaaaagccga
    22501 ctgataagcg cttggggaga ttcacaatcg tttttcaagc ttcattcaat tttaggtggt
    22561 tactatgcct ggattacttg ggatgggaaa aaactagacc ccgaactagc agaaaaagta
    22621 gtagatatgg gtcttggaat tgcaccaagt ctactttttg ggcaagatat aaatggaatc
    22681 agaatcaatt acagtaggct ggatgaaaac caaataccgt tgttttattc aaagatgaaa
    22741 attctagaaa agtggttaac agaataattt cacaaagtaa atagattaaa gaatataaca
    22801 gcttggattt aaatttcaag ctgtttcttt tttttcggaa aaagaatacg atataaagaa
    22861 actaagaaaa aaccattatt tgtacgtccg ttcacgatgt gtaaataaaa taaatgtttg
    22921 caacaaaact gtataacacc aacgttttta aatagaaaca gatggttgta agtttgatta
    22981 gaaaatagaa aaaatgcaga tgaaatataa aacgctttcc ttattgtgat tttattcact
    23041 aatgctgtta atatgcggat tggcataatt tcctttcatc gtgaaaattt gtgcttttag
    23101 cttgtatttc tcataaatcc atgataattt attaatgaag acggttaaat agtgatacag
    23161 ttttattcac tgtttcaatc gcagaatgaa gagattttct tatagatgag agaggaatgt
    23221 gtgacaaatg gcaaacaaaa aagcaccgat cgacttccaa gcactgctag atgaagtcaa
    23281 tgcagatttc ccagtatatc aagcacttga tcaagatgga aacgtagtta acaaagattt
    23341 agtacctgat ttatccgatg atgaactcgt tgaattaatg actcatatgg tatggtctcg
    23401 tgtattagac caacgttcaa cagcgctgaa ccgccaagga cgcctaggct tttttgcacc
    23461 aactgctgga caagaggcaa gtcagctggc tagtgcttac gcatttgata aagaagatgt
    23521 tcttcttcca ggttatcggg atgtacctca actagtaaaa catggtttac ctttatctca
    23581 agctttttta tggtctcgag gacatgctgc aggtaattct tatccggaag aattgaaagc
    23641 tctaccacca caaattatca tcggtgcaca atatgtacaa gcagccggtg ttgcattagg
    23701 tttgaagaaa cgaaacaaga aaaatgtcgt cttcacttat actggtgatg gcggttcatc
    23761 acaaggtgat ttttacgaag caatcaattt cgctggcgct taccatgcaa atgccgtatt
    23821 ttatatccaa aacaacgggt atgcgatctc tacacctcgt gaaaaacaaa ctgcagcaaa
    23881 aacacttgca caaaaagctg tcgcagctgg gattcctggt attcaagtcg atggaatgga
    23941 tccgcttgca gtatatactg tatctaaaat ggcacgggaa tgggcgatca gtggaaatgg
    24001 accagtccta atcgaaacac tgacttatcg ttatggacca catacactat ctggtgacga
    24061 tccaacccgc taccgtacaa aagacacaga tgacgattgg cagaaaaaag atccattgat
    24121 ccgcttccgt aaatatttga cggaaaaagg actttggtct gaagaaaaag aagaacaagt
    24181 catcgaagca actaaagaag aaatcaaagc agctattgct gaagccgaca aagtaccaaa
    24241 acaaaaggtt tcagatttct tgaaaaacat gttcgaagta tctccacaat caatcaaaga
    24301 acaaattgca atttatgaag cgaaggagtc gaaataatca tggcacaaaa aacaatgatc
    24361 caagcaatca cagatgcctt agcacttgaa ctagaaaatg acgagaatgt cgttgttttt
    24421 ggtgaagatg ttgggaaaaa cggaggagtt ttccgggcaa ctgaaggatt gcaggaaaag
    24481 ttcggagaag accgagtatt cgatactcct ttagctgaat ctggaatcgc aggattgagt
    24541 ttcggtcttg cgttagaagg atttcgtcca gttcctgaaa tccaattctt tggttttatt
    24601 ttcgaagcaa tggacgaagt agtagctcaa atggctcgta caagatatcg aatgagcggc
    24661 actagaaatc tacccatcac aattcgttcg ccatttggtg gcggtgttca tacaccagaa
    24721 ttgcattcgg ataatttaga aggtttgatc gctcagtctc ctggtattcg agtagtgatt
    24781 ccttccaacc cttatgatgc aaaaggacta ttgatttctt ctattagaag taacgatccg
    24841 gttgttttcc tagaacacat gaaactatat cgctcattcc gagaagaagt gccggatgaa
    24901 gcttacgaag ttcctttgga taaagcagca gttactcgtg agggaacaga tgtatctatt
    24961 atcacatacg gagcaatggt gcgtgaagca atcaaagcag cagacaatct tgcaaaagaa
    25021 aatatatctg ttgaaatcat tgatcttcgt acagttgcgc cattagatgt agaaacgatc
    25081 attcaatccg tggaaaaaac tggtcgcgta gttgttgtcc aagaagcaca acgacaagcg
    25141 ggcgttgccg cgcaagtagt ttctgagatc tcagaacgcg ctatcctttc attagaagcc
    25201 ccaatcggac gagtttctgc acctgataca gtcttcccat ttggacaagc agaaaatgtc
    25261 tggttgccaa atgcaaaaga tatcgaagac aaagtaaaag aaatcgcgga attttaagaa
    25321 aagaagggac gaaacaaagt catggcttat caatttaaat t
//
LOCUS       DACKAC010000041        25255 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.041, whole genome shotgun sequence.
ACCESSION   DACKAC010000041 DACKAC010000000
VERSION     DACKAC010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.041"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(2..1990)
                     /gene="treP"
                     /locus_tag="I0B46_001703"
     CDS             complement(2..1990)
                     /gene="treP"
                     /locus_tag="I0B46_001703"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAR2084007.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLVYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            2213..2926
                     /gene="treR"
                     /locus_tag="I0B46_001704"
     CDS             2213..2926
                     /gene="treR"
                     /locus_tag="I0B46_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAR2084008.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            3003..3449
                     /gene="msrB"
                     /locus_tag="I0B46_001705"
     CDS             3003..3449
                     /gene="msrB"
                     /locus_tag="I0B46_001705"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAR2084009.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            3619..4221
                     /gene="ruvA"
                     /locus_tag="I0B46_001706"
     CDS             3619..4221
                     /gene="ruvA"
                     /locus_tag="I0B46_001706"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAR2084010.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            4234..5235
                     /gene="ruvB"
                     /locus_tag="I0B46_001707"
     CDS             4234..5235
                     /gene="ruvB"
                     /locus_tag="I0B46_001707"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAR2084011.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            5264..5842
                     /locus_tag="I0B46_001708"
     CDS             5264..5842
                     /locus_tag="I0B46_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAR2084012.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKVLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            5894..6376
                     /locus_tag="I0B46_001709"
     CDS             5894..6376
                     /locus_tag="I0B46_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAR2084013.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIAIEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            6493..6867
                     /locus_tag="I0B46_001710"
     CDS             6493..6867
                     /locus_tag="I0B46_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084014.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(7545..8897)
                     /gene="brnQ"
                     /locus_tag="I0B46_001711"
     CDS             complement(7545..8897)
                     /gene="brnQ"
                     /locus_tag="I0B46_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAR2084015.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
     gene            9044..9634
                     /locus_tag="I0B46_001712"
     CDS             9044..9634
                     /locus_tag="I0B46_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAR2084016.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            complement(9762..11111)
                     /locus_tag="I0B46_001713"
     CDS             complement(9762..11111)
                     /locus_tag="I0B46_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAR2084017.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(11279..12019)
                     /locus_tag="I0B46_001714"
     CDS             complement(11279..12019)
                     /locus_tag="I0B46_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAR2084018.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            complement(12032..13624)
                     /locus_tag="I0B46_001715"
     CDS             complement(12032..13624)
                     /locus_tag="I0B46_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAR2084019.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            complement(14192..14488)
                     /locus_tag="I0B46_001716"
     CDS             complement(14192..14488)
                     /locus_tag="I0B46_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084020.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            14664..15389
                     /locus_tag="I0B46_001717"
     CDS             14664..15389
                     /locus_tag="I0B46_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAR2084021.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            15382..16224
                     /locus_tag="I0B46_001718"
     CDS             15382..16224
                     /locus_tag="I0B46_001718"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAR2084022.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKAVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            16227..16748
                     /locus_tag="I0B46_001719"
     CDS             16227..16748
                     /locus_tag="I0B46_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAR2084023.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(17001..17564)
                     /locus_tag="I0B46_001720"
     CDS             complement(17001..17564)
                     /locus_tag="I0B46_001720"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAR2084024.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            17815..18084
                     /gene="rpsO"
                     /locus_tag="I0B46_001721"
     CDS             17815..18084
                     /gene="rpsO"
                     /locus_tag="I0B46_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAR2084025.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            18272..20398
                     /gene="pnp"
                     /locus_tag="I0B46_001722"
     CDS             18272..20398
                     /gene="pnp"
                     /locus_tag="I0B46_001722"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAR2084026.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            20908..21174
                     /locus_tag="I0B46_001723"
     CDS             20908..21174
                     /locus_tag="I0B46_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAR2084027.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            21318..23309
                     /locus_tag="I0B46_001724"
     CDS             21318..23309
                     /locus_tag="I0B46_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAR2084028.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            23663..24964
                     /locus_tag="I0B46_001725"
     CDS             23663..24964
                     /locus_tag="I0B46_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAR2084029.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
BASE COUNT         8131 a         4785 c         5184 g         7155 t
ORIGIN      
        1 actaagattt tacttcagct ttaccgactg cttcgcccgg gcgaacaagt cctgattggt
       61 caagagaaag tcctgcaact ttttcattat tggtcaaagc aacaataatg tcagttttct
      121 tgccagcttg ttctatttct ggcaaatcca tttcagcaat cagggtctct ggtgtgacaa
      181 ggtatccttc tttcacgaat acattgaaag gaacaccctt tagttcaaca gtatctaatc
      241 ccatgtgaac aagtacttcc agcccctcat tagaaagaat accaatggca tgttttgttt
      301 caaagacact cgtcacttta ccgtttactg gtgcatagac tttgccgttt gacggttcga
      361 cagcataacc gtcacccatc attttttgtg agaaaactgg gtcatttact tcagttattt
      421 ccttgatggt ccctgcagcg actgcgaata atgtttcttt tgttgcaact gccgtaccag
      481 ttgctgaaac aggttcgaaa gactgtggcg ttgctgttgc gccatttgct gtttgaagtt
      541 gtacatcagc agcattttca ggaacagcag gatcaatctt attcaaaatt ccctgacgtc
      601 taaagatgat cgtcaagcca aatggaatga tgacagcaat gatcattgcg atgatgaaag
      661 gtacccatgt ttctgcttgg atagctagta ttcctggcaa accgcctact ccgatcgcgt
      721 tagctcgaac acccatcaag ttagcaaaca tgccagctaa tccagagccg accattgcag
      781 cgacgaaagg atagacgtat ttcaaattga ttccgaacat agcaggttca gttacgccta
      841 agtaacaaga aatcattgct ggaatcgaga tttgttcttc ttttttattc ccacgatgta
      901 agaagatgat ggctaagact gcagagcctt gggcgatatt tgataaagca atcatcggcc
      961 ataaattcgt tgatccgaaa tctgcaatca gctgcatatc gatggcattg gtcatatggt
     1021 ggagaccagt aatcactaaa ggagcataca ggaatccgaa tacggcacta aacaaccagc
     1081 tgatagaaga cgtcaatcca gtatttacga tatttgagat ccagcttccg actgtccagc
     1141 cgataggacc taaaataacg tgtgcagcta aaacagttgg taacaatgaa aacaatggaa
     1201 caaaaatcat cgagatcgac tgagggatat atttgcggaa gaagatttct agatacacta
     1261 acataaaccc tgccagcatg gctggaataa cttgcgcttg ataaccaatc atctggactt
     1321 gagcaaaacc aaaatcccaa aatggaatat ctgctgcggc tgtggaagct acactataag
     1381 catttaataa ttgcggagaa actaacgtaa ttcctaaaac gattcctaat atttgtgtag
     1441 tacccatctt ttttgcaata ctccaagtga tacctactgg aagaaaatgg aaaattgctt
     1501 cacccggtaa ccacaaaaag gcattgacac cattccaaaa cttcgagtgt tcaacgattg
     1561 ttccaccaag actttcaaac tggatacctt caagtacatt tctgaatcca aggatcaatc
     1621 caccgacaat gattgcagga atcagtggtg taaagatttc tgctaaaacg gcaatagcac
     1681 gttgaacggg atgaaggttt tgttttgcag ctgcttttcc ttgttctttg gaaacccctt
     1741 caactccaga tactgcaacg aagtcgttat aaaaagtaga aacatcgttt cctatgatga
     1801 cttggaactg tcctgcattc gtgaacatcc cttttacgct aggtatatct tcgatcgctt
     1861 tttcgtccgc ttttcctgga tcattcagga caaaacgcat acgagtcgca caatgactaa
     1921 ctgcagcaat attttctttt ccgccgatat catgtaacag cttctcggca tctgcttgat
     1981 attttgccat atcaataaac ccctttctct taacttgtct atgcaagttg tttcttcatt
     2041 tactatactt tttttcggag agagttgcaa gcgttttatt aaaaagtacg agagaaaatg
     2101 atatcgtttt aaaaagctta aaataagctt ttcttaaaaa taggaacatt gaaatacaaa
     2161 aagagctgta gtatagtgaa gaaatgatgg aagaacgagt gagggaaaaa acatgaataa
     2221 gttcaatgaa atttttttag atttggaaca gaaaatttta gcgaaagaat atcctccgca
     2281 ttcgctttta cccagtgaga accaattgat caaaatttat ggtgtttcac gagaaacaat
     2341 tagaaaagcg ctgaatcttt tgactacgga aggttatatt caaaaaaaac aaggaaaagg
     2401 ttcgatcgta ctggatcgga atcgcttcga ctttcctatt tctggattga ctagctataa
     2461 agagctacag gagacccaac gtattccaag tgaaacgatc atccatacgt tagaagaaac
     2521 agaagtgaca aaagcattga acgagatcac tggctgggag atcggtgcgc ctgtttggca
     2581 tttgatacgc gaaagaaaaa tcaatggcga agtagttata ttagacaccg attatctatt
     2641 aaaggaaatc gttcctattt taactccagc acaagcacaa ggatcgatct atgaatattt
     2701 tgaaaatgag ctttctttga cgattgctta cgcgcaaaag gagatcacag tagaagaagt
     2761 aacggaaatc atgaaaacag cgatggatct tcacgatgac acttatgcag tagtagtcag
     2821 aagcctcgtt catctggaag atacccgctg tttcgagtat tctgagtcgg ttcaccgatt
     2881 agataaattc cgtttcgttg agtttgcccg acgccgaaaa gtgtaaatag ttcaaaagaa
     2941 agcaaattgt tggcatctgt ccactttggc gctatgatag gatcacaaag ggggcatttt
     3001 taatggataa accaataaga ccagaaaaag aagaactgaa agaaaagtta tcacccatgg
     3061 cttatgcagt cacacaagaa aatgcaacag aacgaccatt tagcgggaaa tatgatgatt
     3121 tttacgaaaa ggggatctat gtagatatcg tcagcggtga accgttattc agctctgcag
     3181 aaaaatatga tgcaggttgt ggctggccat cattcagcaa accaataacg aaaagacaag
     3241 tgcgcgaaaa agccgatttt tctcatggca tgcatcgaat agaagttcga agcaaagaag
     3301 cagattctca tctcggacat gtgtttaccg atggaccagt agatcaaggt ggtctacgtt
     3361 actgcatcaa ttctgcctca ctgaaattca ttccatatga aaagatggat gaattaggtt
     3421 atggggaatt caaatcactt gtagaataat ggaaatacac aaagagcggt acaatacggg
     3481 gatttactcg atcgtccgct ctttttttat tcaagcgatt gctatttgaa agaaacgctc
     3541 cccagactcc ttttttcaca aggtgaaatg tgctacaata aagggaacgt atgtgtgcaa
     3601 aagaaagagg actagaatat gtatgaatat ctgacaggtg tggttacctt tatcaacccc
     3661 tattacatag tagtagaaac caatggtatc ggctaccaga tcgcactagg aaatccttac
     3721 cgctacagta gcaaactaaa tcaaaaaata aaaatttatg tccatcaggt aatacgtgaa
     3781 gatgcgcatc ttctgtatgg atttgattcg ctagaagaaa agcaactgtt tttgcgatta
     3841 gtaagtgttt caggaatcgg tccgaaaagt gcccttgcaa tcatggcaag cgaagaccac
     3901 tccggtttga ttcaagcagt agaatccagt gatgtcactt atttgacgaa atttccagga
     3961 gtagggaaaa agacagcaca acaaatgatc ttggatctaa aaggaaaatt cggtgagctg
     4021 agtatcgata caccttttag tctatttgat gaagcaaaca caaaagacgc tacagctcta
     4081 tctgaagcaa tggaagctct ctcggctctt ggttacagtg atcgagaaat caaacgtgta
     4141 gaaaaacaat taaaagaagt agataatcag acaacggatg agtatctacg ccaagcactc
     4201 aagctaatga tgaaaaaata aggaggagac caaatgacag atgaatactt gttatcacca
     4261 gaatctggcg aaaatgaact gagtttggaa aaatctcttc gaccacagct gctttctcaa
     4321 tatatcggac agagaaaagt caaacaagaa cttcagattt acatcgaagc tgccagaaat
     4381 cgggaagaac ctttagatca tgttctgttg tacgggcctc ctggattagg aaaaacgacg
     4441 atggccatgg tcattgctaa tgagatgacc gtaaatattc gtacgacgag tggccctgct
     4501 atcgaacgtc cgggtgactt agtagcaatc ttgaatgaac tggaagcagg ggatgtcctg
     4561 tttatcgatg agatccatcg attacctaga gtggtagaag agatgcttta ttcagctatg
     4621 gaagatttct atgtagatat catggtagga caagggccaa ccgcacatcc ggttcatttc
     4681 cctcttcctc cctttacttt gattggcgcg acgacacggg caggtatgct ttctgcacct
     4741 ttacgagatc gatttggcat catttctcat atggaatatt atgacgaaca ggatctgcag
     4801 gagatcgttt tacgttcttc ggatattttt caaacagaga tcattgaaga aggggcattg
     4861 gaaattgctc gccgatcacg aggaacgcct agaatcgcta atcgcttgct caaacgggtt
     4921 cgtgattttg cgcaagtcca aggagacggg aaaatcgatc gtcctatagc ggatcaggca
     4981 cttactttgc tacaggtcga tcaggctgga ttggattatg ttgaccagaa attgttgcga
     5041 acaatgatcg agttatatgg cggcggacca gtagggttaa gtacgctttc agtaaacata
     5101 ggagaagagc gtgagacggt agaagatatg tatgaaccgt atctgatcca aaaaggattt
     5161 ttaaagcgta cacctagagg gcgaattgct actgcttatg cctatgaaca ttttggttac
     5221 gactatcata tatgattttt gcttttagat tggaggaaaa tgaatgagtg atacaacacg
     5281 tacgaaacaa ttgatcaaaa acgtattgat tgatttgtgc caaacgcaat cttttcgaaa
     5341 aatcagcgtc caaaaaatcg caaatgaagc aaaaatcaat cgacaaacat tttattatca
     5401 ttttacagat aaatacgatt tgcttcgttg ggtctattgt gaggatgcat tgcattattt
     5461 agatccacaa tgtctttcac ttgataattg ggaagagcaa gcattgaaag tgctgaaagc
     5521 aattgctgag aagaaagaat tttatgcaaa tactgtgctt tctgacccag aaattttaca
     5581 aaaagagttt tctcaactga tccaaaaatc atttatcaag atttttgaac aaatggatga
     5641 agaaagacaa ctttctgcaa gagacaaact attttatgct cgtttttttt cttacggttg
     5701 cagtggtgta ttagtcaatt ggatcacgaa tgaatatgaa gaaacaccga tggaaatcgc
     5761 tatgcagctt ttccgtttag ccaaggatac ggaattatat tcttatcgtc tttatgcaaa
     5821 agaagagttg gaagaacagt aatagaaaaa ttttttgttg gaaaagcaat aatcgctaaa
     5881 aggagatggg gaaatgacaa aaattttatt tgtttgtttg ggaaatattt gccgttcacc
     5941 gatggcagaa ggcttgctga gaaaacgaat agcaatagaa ggaagggaag cagaattttt
     6001 tgtggattct gctgctacaa gcacgtatga agttggaaag acaccgcacc ctggaacgaa
     6061 aaaaatatta aaccaagaaa aagtagatat gacgaatatg gttgctcggc aaatcacacc
     6121 ccacgatttt gaaacttttg attggatcat tggcatggat caagaaaatg tagaagaact
     6181 gaaacgaaga gcaccacgct cagcgcaggg gaaaatccat ttgtttttaa gtagcattcc
     6241 tggaaaagaa aaagaaaatg ttcccgaccc atactatacc aataactttg atgagacata
     6301 tcacttgatc acagaaggcc tgaatcattg gtggaatatt tggattgacg aaaataaaat
     6361 aacaaacaat ttgtaaagtt gacgctttct ttttaaggcg atgttagagc atttccttgt
     6421 ttttttgtaa aatgttaggt aaaataaaat taattaagta acactttctt agctaaagga
     6481 ggagtgcatt cgatgaacga agagacagta tcgccaatga agaagcttac gaagttagtt
     6541 actcatactt cgtatcaact ttttgattct tttgtaaatg acactgaaga acatgctaag
     6601 aatgtacgat tgcaaggaaa gtttattctt gagctgcaaa aagctgctac tgaacatagt
     6661 ctagttatct tacaagtaag agcgaatcgt ttgagtagca agtatgaaac gatctcaggc
     6721 tgggtggcga caaaaacggt cgaaaaagac cgagtgatga tccgcctaca aaacgaagag
     6781 cagcagatcc ggatggttcc tgtagaacaa attgaaaaaa taacaatcct ttcaccagca
     6841 ggggaacagg aacgattatc aaaataattt ataccagata gaaaagcacg tatgagcttg
     6901 taccatcaat cattactaat caaaaataac cgaatgcttt aagctgatat cagtaaaacg
     6961 gaacgtttta ggatgagtat cacagcatga cagcattggg ttgattagtt gaatcgattg
     7021 gatggtacaa gtttttttga taaaaaaagg tcctgctagc cggggagcta gcaggataag
     7081 gagttaaaaa tgaaaaagtg ttgttagggt tgtttgttgg tatgcttata taatagtcac
     7141 tagttgtgaa tattttgtga cgaaagtttt cgaaagttgt taagtttttt tgcgaaaact
     7201 atttttatat catatatatg tctaaaatct atattataaa agaaatgttg tttgatcagc
     7261 ttctcttcgg tattagttca aaacaaaacg gcaaagtata tggagctact ttgccgtttt
     7321 gtttctctaa tactcaggtc agtttataga cgctcaataa acggtgttca aaagccagtt
     7381 tctcggtatt aagtcaaatt tatgcaaaaa tgtgagaagc cattttcaaa aaatatttct
     7441 taatactcga aactgagcgg ctttttcacg acctctcaat aaacagtgtt caagagacag
     7501 cctatcggca ataaggctga acgtctcttt cacaacctct tttatcaatc tgctacttct
     7561 tctttcttag cgaaactcca aatcagtccg ataacaaaac cgataactgc tggtacgatc
     7621 caccccatcc ccaatttgaa aaacggcaag tagctttccg caacatataa aagattttga
     7681 acaataggtg tttgtttgat agtttctggg caagcgttca acccatcaaa aatcgaagca
     7741 atcaatgtaa aataagtagt cattcgataa acagccgtac gctgcttaaa caaaggacca
     7801 actaacacta gtaaaatcag tgtcattgct aatggataga tgaacatcaa aacaggcaac
     7861 gaaaattgaa tgatattcgt caagcctacg tttgccgcta aacaaggaag gatactagca
     7921 agtgcaacaa agaatgcgta atttctttgc ggaaacaaat ctgtgaatgt ttctgcaaaa
     7981 gctgtaatca aaccaatacc ggtcttcaag caagctaata taacgataaa tgctaataag
     8041 atactgccat aagtacctaa ataatgatca gcaatctgag ccagcgtgat cccgccattt
     8101 tcacttagag caaagctacc taagctcata gcacctaaat aagcgagcaa agtataaatg
     8161 attcccatca atataatgct gatcgcacca gatttgatcg tgtctttagc gatttcagca
     8221 ggttttgtta cacccatgtt ttggattgtt gtgacaatga tgatcccaaa tgctaaagca
     8281 gcaagcgcat ctaatgtgtt atatccttga gtaaaccctg tgaaaaaagc atgctcttga
     8341 taatttggct gaataggcgc ttgatttaca gaacctagtg gattcaaaaa agcaaggaga
     8401 agcaaagcac ctaataaaac caaaaatgca ggattcagga actttccaac ataatctaat
     8461 atctttgtcg gttttcttga aaaccaccaa gccagcacaa aaaatacaat actgaaaatt
     8521 gctagatata gcgtctgtct gctaggatcg ataaatggtg ctaaaccaat ctcgaaagat
     8581 gtggttgcaa ggcgaggtaa agcaaaaaac ggtccgataa ctaagtaaag caagatggta
     8641 aaaaccatcg catatgtttg atttatccgt tttgctaatt catatacacc tgagcttttc
     8701 gatacaccaa tggcaatcac ccctaagaag ggaagaccga taccggtaac caagaaacct
     8761 agattcgccc aaaaggtagc agcgcctgct tcttgaccca aatgaacggg aaagataaga
     8821 tttccagcac caaaaaatag accgaaaagc atggagccta taaaaagata gtctttaaat
     8881 gataattttt ttgtcataca taactccttt acggtttttt taaaagtaac aaaatttcga
     8941 aaaatatgca acaattttca gaaaatttcc tatataaaac gacggttatt taataacgta
     9001 agtgataaaa taaaagcgct gaatcatctg aggaggaatc atcgtgacaa agtcgagatt
     9061 agaagcattt actgatggag tggtagccat tgttttgaga gttcttgtat tagagatcaa
     9121 gatccctaat acatctagct ttcattcttt atgggtcatc cgaaatacat tattagctta
     9181 taccatcagc tttatatttg ttgcggtgat ctgggtgaac catcatcgta tatttcagat
     9241 ggcagaacga attaactacc gagtggtctg gtcgaatatc tttttgctct tttggctgac
     9301 tttatgtcct tctgttacta gttgggtcgg gcgtaaccct gaagcttttt ggcctgagat
     9361 ggcttatgtc ttagtataca cgatgtggag tttttctttt ggtatcttga tgaggcaaat
     9421 catgaaagca aataaaccag attcccatgt cgtgagagtt ttgtcaaagg accgtcgaag
     9481 tcttatctcc atgatgatta acttatgctt gattgccggt gtcttttttg taccgttgat
     9541 cgggttattc ggacgttttt ttgtatctgg tatttggatt ttttcctata aaaaagcaga
     9601 tgcctattat cagcggttgt ttccggggaa atagaaaaga ggctgggaca gaagctttta
     9661 actccaagaa ataagccgaa atttcacaaa aatttaagaa gcaattttcg taaattcctt
     9721 cttatttctc gaagctgacc acttctgtca caactacttt cttacttctt acgttcatat
     9781 ttcatatgat agattagtaa tagaagcaag aaccacacag gtgttagtaa taaagcgatt
     9841 ctcgtatctt ttcctaggaa caagacgaca cacacaaagg ccatgtaaag aaataccaga
     9901 taattactaa atgggtacaa cggcatttta aatgaatgag cttttttgcc gaggatactt
     9961 ttacgatatt tcatgtgcgt gtagacaagc atactccaga taaacaagaa acaagtagtg
    10021 gcaatactgc ttactagtgt gaatacttca ctaggcatca cgtaattcaa gataacagaa
    10081 atgaaaatga cgatagtaga aaatgtcaaa gcattccctg gcacatgatg tgttgttaac
    10141 tttttgaatt ttttaggggc actgccttct tgcgctaaag aacgaagcat acgtcctgta
    10201 ctatatattg cactgttgca ggcagaagct gctgcactta atacgactaa attgatcaag
    10261 gaagcagcaa tcgtgatccc aatttccgaa aatacttcta caaatggact gttatctgga
    10321 ttcaagctgt tccatggata gattgccata atgacaaaaa gagatcctaa gtaaaataag
    10381 atgatcctga taggaatatt attgatagct tccggcaaca ctttttcagg attttctgtt
    10441 tctccagcga ctaatccaac taattctatc ccagcaaaag caaaggttac catttggaaa
    10501 gctaagataa atccttttgc accagttgga aagaatccgc catgagacca tagattcgtg
    10561 atactggctg tgccaatatc tgtcgtatag tgggtgagga tcatataagc tcctacgagg
    10621 ataaaggcaa tgatcgctac aactttgatc aatgcaaacc aaaactctaa ctcaccaaac
    10681 aatgagacag ccaccatatt caagcaaaga agaagaccta atgcaatcag ttcaggaagc
    10741 cactgaggaa caccaggcgc ccagtagcgt acatacattc cgatggctgt taaatcggcc
    10801 attgcaatcg atacccagca gaaccaatac gtccagccag tgatgaaagc cgcttttttg
    10861 ccaagatatt ctgcaacaaa atccagaaaa gaatggttat tggtgtttga taacagaagt
    10921 tcgcctaacg cacgcataat gaaaaaacag atgcatccag taataagata ggcgagcaat
    10981 atcgaagggc cagctagctg gatagacttt ccggaaccta aaaacaaccc cgttccaatc
    11041 gctccgccaa tagaaatcag ttggacatgt ctatttttca gtccacgctg cagttcttgc
    11101 tcttcattca tttttaaatc atcctaacta aaaaagttta attacttcca tattaaaaga
    11161 gttttctgaa aaacacaact aagattccaa aattttcaga aatttacctt ttatgagtaa
    11221 ttcataaaaa agaactgcgg taaacaccgc agctctttgt cccaatcagc tattgatcct
    11281 aagcatttaa aatacgtgaa aggaattctt ttgtacgttc ttctttcgga tggacaaaga
    11341 tatcttctgg tgtaccttct tctgcaatca cacctttatc cataaaaatg acacggtcag
    11401 atacatcttt tgcaaattcc atctcatgcg tcacgataag cattgtcaag ccagtatgtg
    11461 ctaagtcttt gatcgtatta agcacttcgc caaccatttc agggtctaac gccgaagtag
    11521 gttcgtcgaa tagcaataca tctggattca ttgataatgc ccgagcaatt gcgacacgct
    11581 gtttctgtcc accagataat tgtgctggtt tggcttcgat ataacgatcc ataccaactt
    11641 tttccagatt ttcgattgct acttttttgg cagtttcttt atctcgtttc aggacagttg
    11701 tttgaccaga catacagttt tccaagacat tcatattgtt gaacaggttg aaggattgga
    11761 aaaccatacc tagattcgtt cgatatttag gtaaattata tttaggagct aatacatttt
    11821 ctccattata aatgatctcg ccgcctgttg gtttttccag taaattgata cagcgcaaga
    11881 aagtagattt accagaaccg gaagaaccga tgatcgtcac aacttcacct ttattgactg
    11941 ttacgctgat atctttcaat acttcatttt caccgaagct ttttcttaga tgtttgattt
    12001 caataatttt ttcattcatg attcttcctc cttattcctg caaatttgaa ttgtcgattt
    12061 cttcaagctt ggtataagct gaaggaccat cgattttctt ttctaccgcc cgcaggattc
    12121 tggtgatcgt aaaggtcatg actaagtaga tgatcccgac gatcgtaaat gtttggaaga
    12181 attggaaatt cgcaccggaa gcagagtttc cttggaagaa caaatcagct acgccgatta
    12241 cacttaaaac tgctgtatct ttgatattga taacaaactc atttcctgtt gctggcaaga
    12301 tattccgtaa aacttgtgga ataacaactt ttctcatcgt ttggccatgg gtcataccaa
    12361 tggcttgcgc agcttcgaac tgcccttcat ctactgcaaa aatcccccca cgaacgatct
    12421 ctgtcatgta agctccagta ttgagagaga caataaacaa agcggcaatc gttcggttca
    12481 atgaaatgcc aaaagcgagt gccagaccgt agaagatgac cattgcttgt accatcatcg
    12541 gtgttccgcg gaatacttcg acatagatag aaagaagtgc atttccgatt ttttggaaga
    12601 agcgagtcag tgcatttcct gattcaggta ccgaacggaa gacaccaatc agtaatccaa
    12661 gtgtagtacc gacaactgtc ccgatcaatg cgatgaacaa ggtaagtcca gcacctcgta
    12721 agaacatatt gccgtattgt ttccaaatcg tagtgaaatc agaaatgatg ccaggttttt
    12781 cttcactatc agttgcagca ggctgatctt tgactgcttg gtccataatt ttggttcgtt
    12841 catcgttaga gatgccagaa agaatctggt ttactttttg gatatccggg tcgcctttac
    12901 gcataccaac agaaatctga acatcttccg ggtttgtctg gaagccatct gcaccgctga
    12961 attcaagcat ttccaagtct ttgttcacac tagttgccgt tacaccttcc ggacgttcgc
    13021 tgacataacc atcgatcatt ccagaagcta aagctgttct cattgctgag aaattatcca
    13081 ttgcttgttg tttctgcaca tctggtatct gatcgatcac agtatagtgg aatgtattta
    13141 actgagcagt aatctttgct ccagaaagat ctgctaaact ttttgctttt gcatatttgc
    13201 tatctttttg aactactact actaattgtg attcgtaata aggatcggta aaatcaattt
    13261 cttttcgtcg ttcagcagta gggctcattc cggcgatgat cgcgtcgatc ttgcctgatt
    13321 gaagggcagg agccagacca tcccatttcg tttggacgat gactaatttt ttccctaaac
    13381 catctgctac ttttttagcg atttgaacat cataaccgcc ggcgtaacta ttttggcctt
    13441 gaattggcaa ggcattattc tcatctgttt gttgagacca gttgaagggt gcatacccag
    13501 cctccatacc tacgcgaaac tcatcgctcg gtgttttttc atctgcattt gccagagatc
    13561 ccacactgaa tagtgagaag acaaaagcaa atagtacggc aactgaaacc atattttttt
    13621 tcattttttt ctccctttct gattatatga tacacgagcg gtctagatga aatgcggtaa
    13681 agctttatct gaaaaaataa aaagcgaccg atttatagat ataaaaaggc cgctaaaatt
    13741 ccataaggtg ataaacctct cacaaaacat agcgcaactt agcatcactg ctaagacagt
    13801 ccgcaagctc ttaacttgcg tcccaacata cactccaagc aaaaaagtgc acatttcggc
    13861 gatcctccct attcctgctt ctccatgttt gcttcactcc acagggttaa tgattctcgc
    13921 gacctctacc tcattgattg aggcatctcg tattcagtta tttatacaaa aaaagtttac
    13981 gatactcgag aggctttgtc aatctataac tgctgttttt tgaaaaaata ctgataaata
    14041 tagaaatctt tacttttacc gaaaaaacaa tcaaaatgtg aatcatctat tcattgcaaa
    14101 cgatagtata attatacaca aaaatgtatg taatacaata aaaaaagaac tagaacagaa
    14161 aatttcctgt gtccagttcc atctcgatca tttaagctgc cgctttcttt ggctcacgtc
    14221 ctaggatcgc ttgcaaaaga acaacgacgc cataaatggc taatattaga taaatcaaac
    14281 ttgagtattg ggtgattccc cagccccatg ttcgatatac ggctaatgta atggctaaaa
    14341 caattgcggc aatcacgttg aataaagcaa aagcccaaag atttccattt tctttgcgta
    14401 caagataaaa aacagagaaa taaacgctta acgctaacca ggcgatacaa gccagaataa
    14461 cgccccaata aataaagaat ggtaacatat cgattcacct cacttttata cagcagtatt
    14521 gtaacacgtt ttcattcttt tgtcagtgaa cattgtgata aattgttcaa atgcccataa
    14581 cactttttta ttcccatttt tctgactttt caacaataaa tttgttcgag tatgggctat
    14641 aatggaaagt gaggtgacaa aacatgaaac aattaacagt agacgaagca gcaaaagaga
    14701 tattagctta tcagcaaatt acatttttga ccggtgcagg ggtatccaca ccttctggag
    14761 tgccagacta tcgatctttg tcaggaatct accacgggat ggagcagccg gaatacttgc
    14821 tgagtcatca agcaatggtg aatgaaccgg ataaattcta tcaatttgtc aaaaagattt
    14881 atcatcctga agcaaaaccc aatatcattc atttggaaat ggcacatcta gcgaaagaaa
    14941 aaaatgtttg ggttgtttcc caaaacatcg acggtttgca cgcaaaggct aaaagcccgc
    15001 aactcgtcaa ttttcatgga agtttgtatc aatgtcattg tcgaaaatgt caacaaacgg
    15061 tagactggaa agaatatctt cacagcgaca aacatcaagc atgcggagga caaatacgcc
    15121 cagacatcgt gttatatggt gaagggtttc aagatggagt catggagcag gcggcttatg
    15181 ccgtcagcca agcagagctg atcgtcattg ttggaacgag ttttcaagtt catccgtttt
    15241 gtgatctggt acagtttcgc cagccgtcag ccaaaatctt agtaatcaat caaacgcctg
    15301 tttatttagc ggagccttat acgtttattc aagaagacgg agcggttgtt ttcaaaaaaa
    15361 tacaagaatt aggagcagat tatgactgaa ttcaaaaccg aaaaagaaaa aatgattgca
    15421 ggcgagcttt attttgccaa tgatcctgag ctggttgccg accgtatgtt tgcaagaagt
    15481 caaagccaaa tcatcaacca agcagaatca gcggaactgc gatcccaatt gttgaaagag
    15541 acttttggcc gtacagggaa aaaaatctat atggagccag ttatcaattt tgattacgga
    15601 tataatatat ttgttgggga gaatttttat gcaaatttca attgtacatt tttagatgtc
    15661 agcacgattg aaatcggtga taactgcatg ttcgctccga atgtccagct ttacacggcg
    15721 acgcatccac tacatccagt gaaacgtaac agcggtctgg agtacgcaaa acccattaaa
    15781 atcggtaata atgtttggtt aggcggagga gttatcgtca cgccgggtgt cactctaggg
    15841 gacaacgtag tagtcggcgc aggttcggtt gtgaccaaat cttttcccga caatgtagtg
    15901 attgcaggaa atcctgctcg tatcatcaag gcagtagagg aagaactaac agaagagtct
    15961 cttgaaacat tacgtcataa gatcgatatg atcgatcgag agttggttgc tttattagaa
    16021 aaaagaatgg atacggtaac taaaatagga caagtgaaaa aagaagaaaa ccaagcagtt
    16081 tatgatgcaa aaagagagca gcaagtattg gataaagttg ttagtctgct gaaaaataag
    16141 ggatataagg aaacaataac agatacgtat gttgatttga tgaaacactc gcgcgaatac
    16201 caaaacaaaa tgaaggagga ataagtgtga tcgaaggaga gacacggcga aaacaaatca
    16261 tgcaaacctt ggcaaataca gaaaaaccag tgagtgctag caagtttgcg aaaacttttg
    16321 gagtcagccg tcagatcatt gtaggcgata ttgctttact acgagctgca ggtaaaagta
    16381 tcgtggcaac agcaagaggt tatatcctcg aaacagaaga aaccaatact gggcacatca
    16441 gtaaaatcgc tgtccagcat ggaaaagatc aaacagaaga agaactgaga ctaatcgttg
    16501 aaaatggtgg tgaaatcatt gatgtaattg tggaacatcc cctttacggt gagttagcag
    16561 gaactttgca catcaaaacg ccacaagata tcgattcctt tatgagacgt tatcaaaaaa
    16621 gtaatgctac tttgttatcc gaattaacag gcggcatcca tctgcatacg atccgctacc
    16681 cagaaaagca tatgctgaaa aacatcaaac aaaaactagc tgaagcagga atcctctacg
    16741 aaggataaca tcgtagctgc tactttttat cctagaaact attctatcat ctctgagata
    16801 aacacccata tggaagaaat aagaaaaaat aagaaaagat gcgtatccaa tacacagaat
    16861 tttttggaat tgcgatttct agatgtctat ctaaatttcg attagtggag aaacataaaa
    16921 aagggaagtg cagcttttga gcactcccct tttttataaa gctttcttgt agcatagtat
    16981 cgaaggaacc tgccaattct ctactcgatc accaatacgc cttcttttag tgcaaatgga
    17041 ttttgatcgt tgatgtggtc atagaacatc acaccgttga tatggtcgat ttcatgttga
    17101 acgacgatcg attcataatt cttcaaacga atcttgtgtt tttcaccatt catatcataa
    17161 taagacacag tgattttggc atggcgaaca acatatcctg gtacttctcg atcaacagat
    17221 aaacagcctt caccttcccc taaacaagca tcttgaacag aatggctcaa aattttagga
    17281 ttatacataa cagtacttag agaaggtcca tctgcctctg ggtcaggact tggtacgtga
    17341 acagcaatga ttcttttaga aatatctaat tgaggagcag ctaagccaac accgcctcgt
    17401 aagtgtagtt cttcagcttt gattggatct tggctgtttt ttaagaactc aagcatctct
    17461 tttcctaaag agatatcttc ttcgcttaat ggaagagaaa cttcttttgc aacttcgcgt
    17521 aaggtcggat ttccttcacg aatgatatca tccattgtaa tcatggaata tgtacccctt
    17581 tcttaaaaga ctgtgtttca agtctatttt cgcattatag tttaccacaa aaatggaaaa
    17641 gtagtagtaa gattgtttga aaactacctt ttcttaatga ttaactactt gattttcaac
    17701 ccggtttcag gtaaagtata gctgtgtgct ctaggcacat gacctgccac ttggttaagc
    17761 gaatcaccaa cgttttactc agtcgcaggg gaataatcga aagaggtgaa agaaatggca
    17821 atttcaaaag aacgcaagaa tgaaatcatc aacgaatacg ctcgccatga aggggatact
    17881 ggttctccag aagtacaaat cgctgtatta actgaagaaa tcaaccactt gaatgaacat
    17941 gcacgcgtgc acaaaaaaga ccatcattca taccgcggcc taatgaaaaa agttggtcac
    18001 cgccgtaact tgttagctta cttacgtaaa actgacgttc aacgttaccg cgaattgatc
    18061 aaacgtttag gcttgcgtcg ttaattttag tcaaaaagag tgggattcac atgaatctca
    18121 ctttttttac tcgtatatca attaagtaaa attccgtgat caagctagat ggactcctac
    18181 taaccaaatg aaagctgctc aaaagacggc tttcggcagt tttcatttgt ttagtagcgt
    18241 cttgctttca cgagaaggaa aaggagaagt tatgtcagaa aaacaagtat tcaaaacgac
    18301 atggggcggc cgtcccttac aaatcgaagt cggtcagcta gcaaaacaag caaacggagc
    18361 cgttttagtc cgttatggag atacagtagt cttaagtgca gctgtggcct ctaaagaagc
    18421 aaaagataca gactttttcc cattaacgat caattatgaa gaaaaaatgt atgcagcagg
    18481 gaaaatccct ggaggtttca tcaaacgaga aggccgtcca agtacggaag caacattgac
    18541 tgcccgtttg atcgaccgtc caattcgccc gatgtttgca gaaggcttcc gtaatgaagt
    18601 ccaagtaacg aacatcgtga tgagtgtaga aacagactgc tcgccagcta tggcagcaat
    18661 gctaggttct tctttagcac tatcaatctc agatatccca tttgacggcc caatcgcagg
    18721 cgtagaagtt ggtcgtgtaa atggcgagta cgtcttgaat ccaacagtag aacaagcaga
    18781 acaaacagat atcgaattga ctgttgccgg aacgaaacaa gccatcaata tggttgaaag
    18841 cggcgcgaaa gaagtttcag aagaagacat gcttggtgca ttgctgtttg gttttgacgc
    18901 tatcaaagag ttagttgctt tccaagaaga aatcgttcaa gcagtaggga aagaaaaaat
    18961 ggaagtgact ttactgcaag ttgatgaagt attgaaaaaa gaaatctttg atgcttctta
    19021 cgctacaatg aaagcagcag taatgactga agaaaaactt gctcgcgaag ataatatcga
    19081 gcaggtgaag atcgatatcc gcgaagctta tgctgagaaa tttgcaggtc atgaagatga
    19141 agatcacttg ttgaaagaag ttaaacaaat cacagaagat cttgaaaaag atgtcgtacg
    19201 tgaattgatc accatcgata agatccgtcc tgacgggcgt aaattggatg aaatccgtcc
    19261 attgtcatca gaggtcagct tattgccacg tgtacatgga tcaggtttgt tcacacgagg
    19321 acaaacacaa gctttatcag cttgtacact cgcacctcta ggagagcacc aaatcattga
    19381 tggtttaggg gtggaagtca gcaaacgatt catccaccac tacaatttcc ctcaattttc
    19441 tgttggttct acaggacgtg caggctctcc agggcgtcgg gaaatcggac atggcgcatt
    19501 aggtgaacgt gcgttagcac aagtaatccc ttcagaagaa gaatttcctt atacgatccg
    19561 tttagtagca gaagtattgg aatcaaatgg ttcttcttcc caagcaagta tctgtgctgg
    19621 cacattagca ttgatggatg caggtgtacc aatcaaagcg ccagtcgccg gtattgcgat
    19681 gggacttgtt tctgatggtg aaaactatac gatcctaact gatatccaag ggctggaaga
    19741 tcatcttggc gatatggact ttaaagttgc cggtacaaaa gatggtatca ctgctttaca
    19801 gatggatatc aagattcaag gaatcacaga acaaatccta actgaagcat taactcaagc
    19861 aaaacaagca cgtatggaaa tcttagaaga attgacttca acgattgctg ctccacgtga
    19921 agaattaagc caatatgcac caaaaatcga aatgatccag atcgaacctg ctaaaatcaa
    19981 agatgttatc ggtaaaggcg gcgatacgat caacggaatc atcgatgaaa ctggtgtgaa
    20041 aatcgatatc gatcaagatg gaaaagtaag tattgcttca gctgatgcag aaatgatcaa
    20101 aaaagctatc aagatcatcg aagatttgac caaagaagtc aaagttggtg aagtttatct
    20161 agggaaagtt gtccgtatcg aaaaattcgg tgcattcgtc aacttgatca aaggaaaaga
    20221 tggacttgtc catatttccc aattagcaaa cgatcgtgtc aataaagtcg aagatgttgt
    20281 taaactaggt gatgagatcc ttgtgaaagt aactgaaatc gataaacaag gacgcgtaaa
    20341 tctgtcaaga aaagcaatgt tgaacgaaga tggttcaacg aaaaatgaag aaaaataatt
    20401 taattcatag gaataatgta cttgtgatcg tgaaggtgac agggggacaa taattattcg
    20461 aaaaaagggg actgtgacaa aagccgtgtc acagtccttt tttttcgaat aaacggtgtt
    20521 caaaagcttg catctgcggt aataagtcca gccaagttaa aaacattagg agctgttttt
    20581 acttggctgt tcttattact tgatgcagaa cagctttttt acaacctctc caataaacga
    20641 tgttcaatca gctgtccctc gatattagct caaaaaacgg caaaatatga ggaactattt
    20701 ttccgtttgt ttctctaata ctcaggtcaa accgctgatt tcacaacctc tgattcacgg
    20761 tgttccagaa gcagcttcta cggaaataag ctgaacactt ttgttacaac ttcttttttt
    20821 ctggagtttc ttggagcata tttttggagt ccttctgaat aaagcgtaaa ataaaagtga
    20881 gaaatatgga taaaggaggg aaaggagatg ctgaattttt tatggtcgtt atttatcggc
    20941 ggtatattag gtttgatagc tggcgcaatt ttaggaaagg aagtcccggg aggcgtatct
    21001 ggaaatatca ttatcggttt tttaggtagt tggctcggcg aatttttact aggaccactg
    21061 ggaccggaac tcggcggttt ttatttgata ccagcattat tgggtgcaat tttttgcctt
    21121 gctctttatt catttgtggc aagacgtatc agagaacgtc caaagaaaga ataattttat
    21181 tcaaataagt tgacacttca gtgtgttact tggataatta gaagcaattt aggaaagcgg
    21241 gtgtcatgga taagaatatc atctatcatt caagcttacc taaaggatat ccatataaca
    21301 agaaatgagg gaaaaacgtg gcaacaacgc acaataccaa aaagatatca atggctggcc
    21361 tgcttgtagc gatgggagtc gtctacggag acatcgggac aagtccgctt tacgttatga
    21421 aatcgattgt tgaagataat ggcggattgt caggaatcaa tcctgatttt gttatcggat
    21481 cagtctcatt gattttttgg acattgactc tactgacaac aatcaaatat gtcgtgattg
    21541 cattgaatgc agataaccat ggtgaaggtg gaattttctc actctacaca cttgtccgaa
    21601 aaggtgggaa atatttgatc attcctgcaa tgatcggagg agctgcctta ctagcagatg
    21661 gggtattgac ccctgctgtg acggtaacga ctgcgattga aggcttgcga ggaattccgc
    21721 agttttttga ccggttcggt aataaccaga acgtgatcgt cattatcact ttagttatta
    21781 tttttgtttt attcatggtc caacgctttg gtacagatat tgtcggaaaa gcattcggac
    21841 caatcatgtt cgcttggttt acttttttag gcgtaatggg attaatgaac ttcagtcaag
    21901 actggacagt tatccgtgca ctgaatcctt attatgctat ccagctgctt tttagtgcag
    21961 ataacaaatt aggcttgttc attcttggaa atattttcct ggctacgaca ggtgcagagg
    22021 cgctttattc cgatcttgga catgtcggca aacgaaatat tcgtctgagc tggccttacg
    22081 tcaaaatttg tttggttatc aattattttg ggcaggctgc gtggttgttg aatgtttatc
    22141 aaaaccctga agcacagcaa atcaagaacc tgaatccatt cttccaaatg atgcctcaaa
    22201 gttggatcgt gatcggcgtt gcttttgcaa cagtcgctgc agtcattgct tcgcaagcat
    22261 tgattacagg ttcgtttacg ctagtttcag aagcaatcaa gttgaaactg ctgcctcggt
    22321 taaagatcat gtatcctggg aacagtatcg ggcaaatgta tatcccagca gtcaatctga
    22381 ttttatggat cgcctgttct ctagtggttg tcacattccg aacgtctcat catatggaag
    22441 ctgcttacgg tctatcgatc actgtgacga tgttgatgac aacagcactg ctttactttt
    22501 atcttatcca aaatgggcat tctcgttggc ttgcatattt agttaccttt ttcttcggtg
    22561 cgatagaagt cgttttcttt atctcaagtg ttgtcaaatt cttccgaggc ggttttgtag
    22621 ccgttctgat tggtttgatc atcttagtcg tgatgtttat ttgggaacaa gggaacatta
    22681 ttcgcgaatc gtcaaccgag gatgtgtctt tgagagatta tatcccgcag cttagagaat
    22741 tacgtgatga tacttcttta ccaatctatc agacaaatgt ggtcttcttg gttcctgata
    22801 tggctgaaga taaagttggc cgacagttca tctattcgat tttagataaa cggcctaaaa
    22861 gagcacgagt ctattggttt gttcatgtgg aagtgacgga tgagccatat acgaaagaat
    22921 atcaagtaga tatgatggac actgatttta ttgtccaagt gaatctgttc ttaggcttca
    22981 gggtccaaca ggaaatcaac gtatatgttc gccaagtcat ccatgatttg atgaaacaag
    23041 gccgtttgcc aaaacagcca caaagatact ctctgacacc agggcgagaa gttggcgatt
    23101 tccaatttgt cctgattgat gaagtagttt ccaatgtgac aactctagga aaatggcaac
    23161 gtcaaatcat gcaggcaaaa ctggcaatca aaaaaatcgc tacctcgcca gaaacatggt
    23221 ttgggctaga gtacagcgaa gtaaaacatg aaactgttcc gcttatcatt ggaaatactc
    23281 ggaaaacatg gttgaaagag cggttgtgaa agtagcgctt tgacctgaac taatgtcgaa
    23341 tgtcagctac ttgaacaccg tttatcagag gtcatgaaaa agctgtactg caacaaacag
    23401 taagaacgag cagataaaaa cagcttctca tgtttttcat ctgtttgggc ttaatgccgc
    23461 agatgcaagc ttttgaatac cgtttttatc agaggtcatg aaaaagctgt ttagcaacaa
    23521 gtaacgcaat gattttgaag tgataaggag atgtggctga aaaatgccgc atctcctttt
    23581 ttattcctaa atatgaatac gcattatttt caatcttttt gtagaaaaag ggtacaatag
    23641 aaaaagagaa aggcgggaga atatggttat caatttcatt attttatttt tgatgattgc
    23701 aattacttca ttttttgtag caagtgaatt tgctttagtg aaaatcagac gatcaagatt
    23761 ggaacaacta gaaaaggaaa atgtaaagaa cgccaagcta gctcttcatg ttacacacca
    23821 tttagataac tatttatcag ctagtcaatt aggaattacg ctaaccggtt tgatcatcgg
    23881 gtgggtcggt gaaggatctg tcgcggcttt actagaacca gtgatcggta aactaccttt
    23941 tagttcggct attagcagta caatctctgt ggcgctggga tttattttag ttacgtatat
    24001 cgatgtggtt attggcgaac tgcttcctaa gagctatagt atcgtcaata cggagaaggt
    24061 cgtcttgttc gtagttaaac cacttcatta tttttataaa gtaatgtttc cttttatttg
    24121 ggtattgaat cattcagctg cagggctagg gaagctatta ggtgtgcgtc tcgtatctga
    24181 aggagaagag acattgtctc aagaagagct gacattagtc gcattgaatt cttatgaaaa
    24241 aggagagttg accaaagaag aatatcatta tttggaaaac gtgtttgaat ttgatgatac
    24301 cttggcaaaa gatatacaag tagaccgtac gtccatgcaa gtattcgaag cttcagaaac
    24361 cgtcaaagaa gcgataaaag cttcgattga acaaggacat actcgctatc cggttatctt
    24421 agaatcaaaa gacaatgttt taggttatgt caccttgcca gatttgatca agcaatctta
    24481 taaagatgac cagctgacag tagaacagtt gattgaagag ccgatcgtaa caactgaaac
    24541 gatccctata aaaaaattat tgacgatcat gcgaaaaaag ggaaagcata ttgctatctt
    24601 aaaagatgaa tacggaggga caagcggctt agtgacgatt gaagatattt tagaagaaat
    24661 cgtcggggaa atccgagatg agacagattt ggacgaagca ttgatagcgg aacagtcaga
    24721 tggctcttat attatctccg gcaaactaac attagatgat ttccagcgtt attttcatgt
    24781 agagattcca gagtttgaag aaacgaattt tactacactt gcgggctttg cttctagccg
    24841 atataaagaa ataaaagcag gaacaatcat tgagattgca tcattccgtt tcacggtatt
    24901 agaataccag catgcacata tcgattattt caaagtagag tccacggaaa gaaaaacaga
    24961 ataaaagaaa aaaggctgtg acattttttg tcacagcctt tttcctacga ttagtcaagt
    25021 aaaaggtgca agaaaaatag ctcgtaaacg aaagcgacaa aatcaaagca caccaaataa
    25081 agccccgttc gtaaaaaatt ttcgatctgg gcaaagaagt ggtatactgt aagtggttta
    25141 gttaatggtc cactgagacc gcgtacgcat agtcgtactc tgtatgtgcc ctaaccatag
    25201 cgtgcataca tttcagaagc ttgttcatac aagcaactgt ggcaacctta tcttt
//
LOCUS       DACKAC010000042        24664 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.042, whole genome shotgun sequence.
ACCESSION   DACKAC010000042 DACKAC010000000
VERSION     DACKAC010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.042"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            236..1042
                     /locus_tag="I0B46_001726"
     CDS             236..1042
                     /locus_tag="I0B46_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAR2084030.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            1054..2274
                     /locus_tag="I0B46_001727"
     CDS             1054..2274
                     /locus_tag="I0B46_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAR2084031.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     NKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            2264..3850
                     /locus_tag="I0B46_001728"
     CDS             2264..3850
                     /locus_tag="I0B46_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAR2084032.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVVQTSIAQPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSEAT
                     ERMVFRFAQQPSFIEFSAEAAGEKLIFIDSNAVVKTVNDCYLFDGKEIPEFALTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENQHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKKTSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSRDEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            3889..6027
                     /locus_tag="I0B46_001729"
     CDS             3889..6027
                     /locus_tag="I0B46_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAR2084033.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKIRLSGKMRGKAILDFQTAKHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKTIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNIHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKKLGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            6315..7706
                     /locus_tag="I0B46_001730"
     CDS             6315..7706
                     /locus_tag="I0B46_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAR2084034.1"
                     /translation="MNKNGEWNAARRIFIIIMDVLVLNVSIYLSFLLKFQGEVPARNL
                     ETFQHSAVFISIIFIALNILLGAYVFYSRVISDIVFITVIGQVLIALGIMVVTFAGRW
                     FAFPRGVIVLSFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVAPAIQNFQSK
                     KNNKHKVEAVVIDKYYENTKKLIDDIDIVYLASKIPEDEKINIYQLVTKKEKKLFLNT
                     TFENLTMINPNIMNFEDESIIEASGFKIQPEYELFKRLFDIIVSLILLILASPFMLIT
                     AILVKTTSPGPIIYKQVRITKNQKEFSIYKFRSMSVTAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNEENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLLLDLKILLQTIKILFDKMSSQGLDEDENIRS
                     LAEIDFSQEKIYR"
     gene            7765..8574
                     /locus_tag="I0B46_001731"
     CDS             7765..8574
                     /locus_tag="I0B46_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAR2084035.1"
                     /translation="MLLSLYDKEKPEYLKEALTSIFEQTVMPKEIVLVYDGPINEKLQ
                     VVVTYFQQKDPHLFTVVKLEKNQGLGIALAIGLEHTKNRLVARMDTDDIMEPQRLEKQ
                     LEIFQKDPEIAIVGSNIEEFTGTLSNVIGRRIVPEYNDEIRVFSKRRNPFNHMTVMFD
                     KNAILEVGNYRPLIGFEDYYLWVRLLKAGYKGYNIQEILVHARAGADMYARRGGSKYL
                     LPGIKGRYRVWKDGLGSLKDFLIVICGHLVISLLPNKIRGKVYESKLRKTN"
     gene            8546..9535
                     /locus_tag="I0B46_001732"
     CDS             8546..9535
                     /locus_tag="I0B46_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAR2084036.1"
                     /translation="MNQNLEKQTKLSIIIPFYNAKNTLKRAVDSVLTQGYPNIEILLI
                     DDGSTDGSSDIGDEIANLESKVACYHLENAGPSVARNCGIKEATGNLVGFLDSDDYFL
                     PDCFNNVLKDYHRNYPDIIAFGLSKGKNIENANLFVPEKKIGCSSEEAIEQMFRSKAV
                     DFYVWNKFFKRELFNHIVFPENKLYEDMVPMYEAFRAASKIDILPFSGIFYYQNNDSI
                     VYQSFNSKQYDNILQRKILVDEVKKDYPRLINLANARLVDGYLSTGFKITGKNQNQKE
                     KQKYYNQSKREIKSIYSKIRSNNEISIAKKLALFLYLINPKTYFILYKKVLKK"
     gene            9649..10644
                     /locus_tag="I0B46_001733"
     CDS             9649..10644
                     /locus_tag="I0B46_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAR2084037.1"
                     /translation="MHKISVIVPAYNVEDYLENCLESISNQTHSNIEIIVVNDGSPDR
                     SGEIAEEYKKKDMRLKVIHQVNGGLSAARNSGLDSATGDFVCFIDSDDWIEPDYLELL
                     YFGTQTYGADISVLKIKKISSLEQLKEHTIAERKWEIFDREQAMKNLFINNKIGYSAN
                     NKLFNRNLFDNIRFPLGKLMEDKATTYLLIDHSEKVAVNSSAKYHYYQSPKSILRGSF
                     NPRIFDSFEVHEDILIFIDKFYPELSINVRTRYVYEAIRMMMTMIKNNYTNKEDYLRS
                     INILQRFEKEVKIDPDFSPKLKKLVKFLLINKNLPYYMSSSKIASKFLIKIESVR"
     gene            10648..11790
                     /locus_tag="I0B46_001734"
     CDS             10648..11790
                     /locus_tag="I0B46_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid biosynthesis protein F"
                     /protein_id="HAR2084038.1"
                     /translation="MKNIKKFFSRNIFPVFIKILPIQKNLIVFSSFGGRNYSGNPRVI
                     SEKIHEKKLNYRIIWLIKKEAIITDLPDYIKRIDSDSFLAAFYLYTAKYWIDDSRKTI
                     KYAKRKKQLYFQTWHGTPLKKIEFDAKDKLPKRYLSYAKKDSESITYLLSGNRYSSKK
                     YVSAFNIVPSKILEVGTPRNDELASSKEQVFDLKKKQINVLFAPTFRNNIEDNGITQL
                     EWLGVDNLREFFKKQQQELNFMTKFHPNVHSKLATDSKSIEYMKKHQITLINDGIPLE
                     MLFEEIDVLITDYSSIFFDFALTKKPIILFNYDEKEYSLERGFYIQLSELPIISVQDS
                     YGLVDVFKNQQEKLLKSSQELLDYIGNYETGASTEKVLELIERDIK"
     gene            11787..13214
                     /locus_tag="I0B46_001735"
     CDS             11787..13214
                     /locus_tag="I0B46_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAR2084039.1"
                     /translation="MKKTVKDIFYNAIYQVFLIVLPLLTIPILSRRIGSTGLGIYGYV
                     FSISQFLMTVIAVGMNPFRIRNIAKSRKDKKALSLQFWNIYFIQFLIGLSVSFLYIAY
                     IYFFDVKYKNLYLIQLIFIVGLTLDISWFFQGIEEFSKVVIRNTVIKLSSVLLIIIFV
                     STKDDLWIYVFITSITNFLGSLVFWLSIHGKVGKPIFNSNVFKQLWKPAFIILTPQLF
                     MQVYTTLDKTIVGYFVDPTELSYYDQSQKIARIILAVLTSITIVMLPKISKAQAEGKS
                     ENILVYTKKSFDYTIIIALILFSVVFINTKEFVPWFFGENFKPMTINMLISCLIIVVS
                     PIGGVFANQFAIAIEKDKEYAYPMIIGAIVSIIGNVAFVPTYGAVAATIVLVVVELLV
                     FFFRVFFVRKEIKISYFFGKNVILFFIVNLTLTTWAYYFLPQLFSSSFLDMAIKSIMI
                     FIIYLPFVYFLFPDIRNEIRKITRK"
     gene            complement(13281..14570)
                     /locus_tag="I0B46_001736"
                     /pseudo
     CDS             complement(13281..14570)
                     /locus_tag="I0B46_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(14750..15160)
                     /gene="tagD"
                     /locus_tag="I0B46_001737"
     CDS             complement(14750..15160)
                     /gene="tagD"
                     /locus_tag="I0B46_001737"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HAR2084040.1"
                     /translation="MKRVITYGTFDLLHYGHINLLKRAKQYGDYLIVALSTDEFNWNA
                     KQKKCYFSYEKRKQLLEAIRYVDLVIPEENWEQKVKDIKEYHADYFVMGDDWAGQFDE
                     LEQLTDAQVIYLPRTPEISTTQIKNELNSLNSDK"
     gene            15286..16677
                     /locus_tag="I0B46_001738"
     CDS             15286..16677
                     /locus_tag="I0B46_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="HAR2084041.1"
                     /translation="MLNEKFLKYVKGWYFIYFGFILMLRAIGPYLLLPTRYDTLLFWP
                     AGIAGVVLIIIDAIIAFREKNLKKYDWLLIAFIGAMLVSSLMNLKYGYSENIKLIMWQ
                     LIFFFMIYQFGKDYSSNFFFKVFTWILSLVWFLANLVSLYWFVIQYGIKIPIKYKYYP
                     VRLGWLGNRLFGVYTDPNFGAVISLIVIIIAIYYLVTYKHLNLLFKIFFAINIFIQFS
                     FIALSGSRTTLVCLLIATFVFSFFTSYSSRFLKTNTLKRWLTALVVGLLSIIAMYYVT
                     DITAQAWAHVPHITEIKKDISDSSGTTSSNKVDLKRPDTDGGDVSNLRFGLWKSAIEI
                     FKSKPISGGSPGYYIEYAHDKLPYTLMGKDTLTAHNFIFLVMAATGGLGLLTLLTFLF
                     IQCFKTLRYCFTQPNLVKDKHFFAILMILTIAVSALFITELILVSTIGSFIFWGYLGS
                     IQKTISKEEGVTN"
     gene            16810..17757
                     /locus_tag="I0B46_001739"
     CDS             16810..17757
                     /locus_tag="I0B46_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAR2084042.1"
                     /translation="MDTILITGGAGFIGSTLANYLGKENKIVVVDDLSMGKKENLDME
                     KQVTFIEGDVSDPQLMERIMKQYQFAYIFHLAAVASVADSVERPLETHRVNFDSALLL
                     LELVRKYQSGLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFASEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKTEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILNGMERYLKTEID"
     gene            complement(17797..18957)
                     /locus_tag="I0B46_001740"
     CDS             complement(17797..18957)
                     /locus_tag="I0B46_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAR2084043.1"
                     /translation="MAKGKHQKKQQISLSFLAVLLLAVSFFSTGIITGWQLTKQPQPQ
                     IYKTEPAQLASNLTSNEQMLASKIDQLLQKSDYIGSIYVRKNNRVILEKGYGYANLRI
                     KESNSPSLYYQIGSIQKAMTALLILKQIQSGKITFDTKLSEFYPQIPGSQQISIKNML
                     YMRSGLHRTASPKKPMSDSEIIQFALHHLKFTGYDTYHYEPLNYTLLTGVLIKLMNQP
                     YETLLKKEIIRPLHLEHTDFYENVKNSPQHAVSYQMSATDDYSKALVEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGDIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVAAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVNLKS"
     gene            19122..20297
                     /gene="mltG"
                     /locus_tag="I0B46_001741"
     CDS             19122..20297
                     /gene="mltG"
                     /locus_tag="I0B46_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAR2084044.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPNGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEKAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            20433..20912
                     /gene="greA"
                     /locus_tag="I0B46_001742"
     CDS             20433..20912
                     /gene="greA"
                     /locus_tag="I0B46_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAR2084045.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            21080..21811
                     /locus_tag="I0B46_001743"
     CDS             21080..21811
                     /locus_tag="I0B46_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAR2084046.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDVEVIRI"
     gene            21808..22875
                     /locus_tag="I0B46_001744"
     CDS             21808..22875
                     /locus_tag="I0B46_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAR2084047.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEIAKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            22882..23514
                     /locus_tag="I0B46_001745"
     CDS             22882..23514
                     /locus_tag="I0B46_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAR2084048.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDCPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            23654..24319
                     /locus_tag="I0B46_001746"
     CDS             23654..24319
                     /locus_tag="I0B46_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAR2084049.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            24347..24640
                     /locus_tag="I0B46_001747"
     CDS             24347..24640
                     /locus_tag="I0B46_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAR2084050.1"
                     /translation="MELTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
BASE COUNT         8418 a         3663 c         4781 g         7802 t
ORIGIN      
        1 aaacgaagaa aaagtgttag tagaaaatcg ccttcgattt tccaccaaca caaaatatta
       61 tagaacccct tttttggttt attgcaattg tctagcatct tctgtaaaat agaaacgtta
      121 gagaaaatct tgtatcatag aaaattaaga aagaaataag aacgcagctc ggtaccaaaa
      181 atatggtaaa atggcacttg actgtaattc actgaaatcg cttggaggaa cacaaatgtt
      241 taaagatttg tatttattta tgaaagacat ttatcagaat agaaaattac tgctacagtt
      301 ttcgatcaat gacttcaaat caagatatgc aggatcgttt ttaggaatct tatgggcgtt
      361 tatcaatccg ctatttactg tattgattta ttggctggta tttggtgttg gactgaaagc
      421 acaaatgatc gatgcaaaat acccatttat cgtatattta gttaccggga tgattccctg
      481 gttcttcttc tcagatgcat ttgcatcaac tacggttgtt tttcgggaat atacttattt
      541 agtaaagaaa gtcgtattca atattcgtat cttgcccacg acgaaaatcc tatccaatct
      601 gtacacccat ttattcttca ttctgattgg catagctgtc gcgcttataa atggtatata
      661 tccttcagca atgtctttac agctgattta ttatttattt tgcatgtgtg ctttcttgac
      721 aggattgact tggctgacgg catcgatcca accattttta cctgatatca tgcaattaat
      781 tacgatcgtg ctgcagttga tcatgtggac attaccgata ttatggagcc ctaatcagtt
      841 tgattcaggt cttgtaagca tattgaaatt aaatccgctg tactatgttg ttcaaggtta
      901 tcgtgagtca tttttaagtg aagcttggtt ctgggagcat ggaacatata ctttgtattt
      961 ctgggttttc acgttagtaa tgttcgtaat cggctctgca gtgttccgta gattgaaacc
     1021 acatttttca gatgtgctgt aaggagaata gctatgacag aatatgcaat tagactaaaa
     1081 gatatcacaa aatcttataa tatgtacgca aaaccttcgg atcgatttcg tgaagccctc
     1141 aatcctttta agaaatccta tcatgatgta ttttatgcgt tgaaaaacgt gaatgtagaa
     1201 ataaaaaaag gtgagatgat cggtttcgtg ggagaaaacg gatcgggaaa atctacaatg
     1261 ttgaaaatca ttacgggagt actgacacca acatccggca cgatggaaat cgaaggaaaa
     1321 atctcagctt tgctagaact aggatcagga ttcaatcctg agtattctgg ctatgaaaat
     1381 atctatttaa atggaatggt tctaggattt tcaagagaag aagtcgatga aatggtcgat
     1441 gatatcattg aatttgctga tattggcgac catatttatc aacctgtaaa aacctattca
     1501 agtgggatgt tcgttcgtct agcttttgct gtagcgatca atgtgaatcc tgatattttg
     1561 attgtggatg aagcattggc agtaggagat cttgaattcc aactgaaatg tatggaaaaa
     1621 tttacagaaa ttaagaactc gggaaaaacg attttattcg tttctcatga tattagttcg
     1681 attcgccgtt tttgtgatcg ttcctattgg ttgaaaaatg gagaagttgt cgaatatggt
     1741 gatacgatgg aagtaacgac gaattacgaa aactttttga agaaaaaatc agttaaaaca
     1801 gtagatcgaa ataaaaacca agtagaacgc ttcgcagctc cagatatcgt agacgtgacg
     1861 aaagccgaat tgttggataa aaataaaaat ccagtggata tgctggaaca aggagaaaaa
     1921 ctatacgtta aagtaaccta tgaagtaaaa gacgatacaa tcaaaaatcc agttttagga
     1981 attgctctgc gtacagtcga caatttttat gtgtgcggac tcaatactct actagatggt
     2041 atcaagatcc cttggaaaaa aggtgagaat atcttttatc tggagtatga aaagttaggt
     2101 ttgttagctg gtgaatatta ttttgacgtt gcggtctttg aagaaaatgc aacggttcct
     2161 ttggtataca agacaaaata catgaacttg tttgtcagcg gttcttatat cggagaaggc
     2221 atcgtggtct tagaccataa atgggaagaa gggactcata gcaatgaaat atgattttga
     2281 aatggaccta gatgaacaaa gcagtgtggg aaaaattgcc gcacagatca aaccaggaag
     2341 taaagtttta gagtttggtc ccggaaacgg tcgattaacg aaacatttga tcggagcaaa
     2401 acaatgtgaa gtgagcattg ttgagctaga taaggaatta ttcgattttg tcagcgaatt
     2461 tgcccaagat ggattttatg gagatatcga aagttttgaa tgggcaaact attatgctgg
     2521 acagacattt gattacgtct tgtttgcgga tgttttagaa catttagtag atcctggaaa
     2581 aacattgaag aaagtcagag aatttttgaa tgaagaagga gagatcctga ttacattccc
     2641 taacttagca cataattctg tcatgatcga tttgttcaat aatcaattgc cttgggcttc
     2701 ttatggtctc ctagatgaaa cccataattc tttttatact catgatggtt tccaaaaagt
     2761 ttttgaaaaa gccggattat tcattaatat tgaagattac ttgtacttgg ctgtaggaga
     2821 tacagaatta aaatcaactt atgaagagtt gccggaagct gtacgctatg atttcaaaat
     2881 gcgaccattt ggtgaagtct accaatactt tttctcattg atgaaacatc cagtagtaca
     2941 aacttccatt gcacagccac aaaactctaa ttatgtaaaa gtactagaag taacccagca
     3001 aacgaaacaa gatgaaacaa tccaacaaat accattcaat aatttcacag gggaaaatga
     3061 gacactaagt ttccccgtgt cagaagcaac agaaagaatg gtgtttcgct tcgcacagca
     3121 acctagcttt atcgaattca gtgcagaagc tgctggagaa aaactcatat ttatcgattc
     3181 aaatgctgta gtcaaaacag taaatgattg ttatttgttt gatgggaaag aaataccaga
     3241 gttcgctcta acggatattt ctgggaaaga agtgactatc cattgtcact atcgctttat
     3301 cggggaattg acaccaacaa tgaaagaatt gctggaaaca attcgtccta tggctgaagt
     3361 cacaaagcag ctttctgaga aaaatgatga attagaaaga gaaaatcagc atcttcttga
     3421 ggaaaataca agactagacc atacgttaaa aacaaccaca aatcgctatt gcacattgtt
     3481 ggaaagtgat gaatggacga tcaaacatag actaggacga cgtaagaaaa aaacatcgaa
     3541 aaaaatacaa gaaaaagagc tttctatatg tattgatgag aaaatatggg atgcagaaac
     3601 aaaaatatta aaaatcatcg gatggggaat cgctaattcg gatcgccagc cattgtccta
     3661 taagttatca gctgaccaat cgccattttt cgaagcaatt cctgtttcac gagatgaagt
     3721 caatcaagca gaacagctcg caaaaggaac agaagcggga tttgaattac gaatcctttg
     3781 cgagcaagaa cgttcattct tagtggaagc agtagctcaa aatggtcaat catggatcat
     3841 tgaaatgtaa ataatgatta acttataaag ataaggagta aacgaatcgt gcaaaaaaaa
     3901 gaaattgccg tatttatcga tcaaatcact cgtgaacggg ccactggcga tttgcttatt
     3961 gtgggctggg caattgatga agtgaccaaa gaaatcccta cgataaaggt agaaaaagaa
     4021 aatgtgattg ctgaagcaac acatgtcgta agattggata tcaatcattt atataatctt
     4081 gatgttaaga cccaaagcgg ttttaagata cgtttatccg ggaaaatgag aggaaaagcg
     4141 atcttagatt ttcaaacggc aaaacatcaa aatggcatag ctgtgaaact aaatggtaaa
     4201 tacccttatg atgacggcat tgaatcttca tgggaaagaa aaaaaagatt actgaaaaaa
     4261 gggataaatt atgcaaggac tcatggcgta aaaaaaacaa tccgccgggt caaactggaa
     4321 ttgaaaccag gatcgatcga ctatgcagag tggatcagtc gtcacgagaa acctgatctg
     4381 aaagagcaaa ggaaagaagc acaagggttt gcataccgtc cattgatttc catacttatg
     4441 cctgtctata acgttgaaat caaatggctc gaaaaatgca ttgattcggt attggatcaa
     4501 acgtatgatc actgggaact atgtatcagt gatgatgctt ctactgatcc agctatccga
     4561 aagtgtctgg aatcctatca agcaaaggat gatagaatca aagttgtttt ccgacaagaa
     4621 aatggacata tcagtctggc aactaattct gcgttggaaa tggcagaagg agaatttata
     4681 gcgctcttag ataatgatga tgaattaccg ccatttgcat tatatgaagt ggcaaaagtg
     4741 ctaaacatac atccagaact ggatttgatc tacagtgacg aagataaaat cgatgcagat
     4801 ggcaatcgct ttgatccgca cttcaaagca gactggtctc ccgatacatt gatgggaaat
     4861 aattatatct ctcacttagg cgtatatcga acaagtattg tgaaaaagct tggcggtttc
     4921 cgtaaaggct acgaaggctc tcaagattat gatttggtcc ttcgtgtgac agaacaaatc
     4981 cctgcagatc atatttacca tattgaccga gtcttgtatc attggcggac gatcccagga
     5041 tcaaccgcaa gtaatggtga agccaaaagt tatatctatg attctggagt gaaagcattg
     5101 acagatgccc tgtctagaag gaatatcaaa ggaagcgtac gtcctggcag aatttctgga
     5161 ttttatgaga ttgcttatga cgtcttgcaa gaggatttgg tcagcgtgat cattccaacg
     5221 aagaatggct atgaagatct aaaaacttgt gtggattcga ttatcgaaaa aacgtcttat
     5281 ccaaattatg aaatcattat tgcagataat ggaagtacag atcctaaaat gcaggaatta
     5341 tttgcagaat acaagcatca gctgaaagac cgatttatcg ttgaattaat tgacattcca
     5401 ttcaattatt cacgtatcaa caatctggca gcagaaaaag caaacggcaa atacttttta
     5461 ttcttgaata acgatacaga agtgattgaa cctgactgga tgacagcgat ggtttcttat
     5521 gcacagtttg atcgaatcgg ctgtgtagga gcaaaactgt tttatccaga tgatacaacg
     5581 cagcatgcag gagtattatt aggaatcggt ggtgttgcag gacatgcatt gaataactat
     5641 gatcgaacac attgtggtta tttcggccga ttggtcattg acgtcaatta cttagcagtc
     5701 actgcagctt gtatgatggt caaggcagct gacttcaatg ctgtcaatgg atttgatgaa
     5761 acattagaag tagcattcaa tgatgttgac ttgtgtttga aagtttatga actgggtcgt
     5821 tacaatgtct atgcacatca agcagaattg tatcatttcg aatctaaatc aagaggttat
     5881 gaagatacac cagaaaaaca aagacgtttt gccggggaaa taaaaaaaat gcaagataag
     5941 tggccttctt atattgctca tgacccattt tataacgata atctaactaa agaaggaatc
     6001 ggcgattttt cattgagacc tgattgatta aaagaactgt attttcattt ttattgatga
     6061 aaatacagtt tttttagtat atgaaaattg taacttgtga ccgaagagat gataggaaga
     6121 acacccattc agtaggaaga atgtgatacg atttttgtcc aactctctgt taggcagaaa
     6181 tatacatata aacatacaat agtcaccttt tctagtttct tttaatttat tttacaattt
     6241 ggttattcac taggttttta aactttgcta tggtaagatg atagaggcat tattttatgg
     6301 ttaggagtgt atatatgaat aaaaatgggg agtggaatgc ggcacgtagg atattcatta
     6361 tcatcatgga tgtacttgta ttgaatgtgt cgatctatct tagctttttg ctgaagttcc
     6421 aaggagaagt cccagcaaga aacttagaga cctttcagca ttcagcagtt tttatttcca
     6481 ttatctttat tgcgttgaat attttgttag gagcttatgt attctatagc cgagtgatca
     6541 gtgatatcgt cttcattact gttattggac aggtgctgat agcattgggg atcatggtag
     6601 ttacttttgc tggacgttgg tttgcatttc cgcgtggcgt catcgtgctc tcgtttatac
     6661 ttggcacgat attacttagc gtttatcgga tagttgttta taaactatac ttaagagtca
     6721 gtcgtgataa gaaagtcctt atcgtaggtt tggagaaaga tgtcgcacca gctatccaaa
     6781 actttcaatc taagaaaaac aacaaacata aagttgaagc agtagtgatc gacaagtatt
     6841 acgaaaacac aaaaaaattg attgatgata tcgatattgt ctacttggct tctaaaatcc
     6901 cagaagacga aaaaatcaat atttatcaat tagtaactaa aaaagagaag aagctatttt
     6961 taaatacaac ttttgaaaat ttaacgatga tcaatccgaa catcatgaat ttcgaagatg
     7021 aaagtatcat tgaagcttcc ggtttcaaaa ttcagccgga atatgaatta ttcaaacgtc
     7081 tttttgatat catcgtttcg cttattttac tgattcttgc ttcacctttt atgctaatca
     7141 cagcgatttt ggtaaaaaca acttcacctg gaccaattat ttataaacaa gtacgtatta
     7201 cgaaaaatca aaaggaattt tcaatctata aatttcgttc catgagtgta acagctgaag
     7261 caaaatcagg accggtcttg gcaaagagca atgatgcacg tgtgacacca gtaggcaaat
     7321 ttatccgtgc cgtacgtttt gacgaattac ctcaaatctt taatgtattg aaaggcgata
     7381 tgtcgatcgt cggaccacgt ccagaaagac catttttcgt tgatcagttc aatgaagaaa
     7441 atccttatta ttatttaaga cacaatgtac gtgcagggat taccggatat gcacaagtat
     7501 acggaaaata tgtatcagat tataatagta aactacgttt tgatttgttg tatatcaaaa
     7561 agtattcctt actattagat ttgaagattt tacttcaaac aatcaaaatc ttatttgata
     7621 agatgtcctc acaaggacta gatgaagatg aaaatatccg ttcgttagca gaaatcgact
     7681 tttcacaaga gaaaatttat cgctagatgg gaaaaatttt gagcaaagaa gtaggagaga
     7741 acattggaaa ataaaaatat aagtatgctg ctttctttat atgataaaga aaaaccagag
     7801 tatttaaagg aagcattaac tagtattttt gagcaaacag taatgccaaa agaaattgta
     7861 ttagtttatg acggccctat aaatgaaaaa cttcaagtag ttgtcactta tttccaacaa
     7921 aaagatcctc acttgtttac agttgtaaaa ttagaaaaaa atcaaggatt ggggattgct
     7981 ttagcaattg gattagaaca tacgaaaaat cgcttagtag ccagaatgga tacagatgat
     8041 atcatggaac cccaacgatt agaaaaacaa ttagagatct tccaaaaaga tccagaaata
     8101 gcgattgttg ggagtaatat tgaggaattt actggcacgc ttagcaatgt tatcggaaga
     8161 aggattgtac ctgaatataa tgatgaaatc cgtgtgtttt cgaaaagaag aaatcccttt
     8221 aatcatatga cagtcatgtt tgataaaaat gctattttag aagtgggaaa ctatcgccca
     8281 ttaattggat ttgaagatta ttatttgtgg gtaagactgt tgaaggcggg atataaaggg
     8341 tataatatcc aagagattct agtccatgct agagcaggtg ctgacatgta tgctagacgt
     8401 ggaggaagta aatatctttt accaggaatt aaaggccgat atcgcgtatg gaaagatggt
     8461 ctaggatcac taaaagattt tctgatagtt atttgcggac atcttgtaat tagtttatta
     8521 ccgaataaaa ttaggggaaa agtatatgaa tcaaaactta gaaaaacaaa ctaaactttc
     8581 tataatcata cctttttaca atgcaaaaaa tactttaaaa agagctgtag acagcgtctt
     8641 aacgcaaggt tatcccaata ttgaaatctt gttaattgat gatggatcta cagatggtag
     8701 tagtgacatt ggtgacgaaa ttgctaatct agagagtaaa gtagcatgtt atcacttgga
     8761 aaacgctggt ccatcagttg ctaggaattg tgggataaaa gaagcaactg gaaatttggt
     8821 aggattttta gatagtgacg attatttttt acctgattgt tttaataatg ttttgaaaga
     8881 ctaccatagg aattaccccg atatcattgc ttttggatta agtaaaggaa aaaatataga
     8941 aaatgcaaat ttatttgttc ccgaaaagaa gattggttgt tcaagtgaag aagcaattga
     9001 gcaaatgttt agaagtaagg cagtggattt ttacgtatgg aataaatttt ttaaaaggga
     9061 attattcaat catatagttt ttccagaaaa taaattatat gaggatatgg ttcctatgta
     9121 tgaagctttt agagcggcta gtaaaattga tattttgcca ttttcaggta ttttttatta
     9181 tcaaaataat gatagcatcg tttatcagtc ttttaattca aaacagtatg ataatatttt
     9241 gcaaagaaaa atattagttg atgaagtaaa gaaagattat cctcgactta ttaatttggc
     9301 caatgctcga ttagtagacg gatacctttc aacagggttt aaaattactg gcaagaatca
     9361 aaatcagaaa gaaaagcaaa aatattataa tcagtctaaa agagaaataa aaagtatata
     9421 ttcaaaaata agaagtaaca atgagatatc aattgctaaa aaattagctt tatttttata
     9481 cttaattaat cctaaaactt attttatttt atataaaaaa gttctaaaaa aataaagttt
     9541 ttttcgatat gttatttttt atgtttatta acttcggaag actaaaattg ttactggtaa
     9601 tgatacattt gtaaaaataa aaaagatgat ttttaggaag tgaagagagt gcataaaatt
     9661 agtgttatag tacccgcata taacgtagaa gactatttag aaaactgctt agaatctatt
     9721 tccaaccaga cacattcaaa tattgaaata atagtagtca atgatggttc cccggatcga
     9781 agtggggaaa ttgcagaaga atataaaaag aaagatatgc gattaaaagt gatacatcaa
     9841 gtgaatggtg gtttaagtgc ggctaggaat tctggtcttg attctgcaac aggagatttt
     9901 gtgtgcttta ttgacagcga tgattggata gaaccagact atcttgagtt actatatttt
     9961 ggcacgcaaa cttatggagc agatatctcg gtgctaaaaa taaaaaagat ttcaagttta
    10021 gaacaactaa aagaacatac aattgctgag agaaaatggg aaatatttga tagagaacaa
    10081 gcaatgaaaa acttatttat aaataataaa attggctatt ctgcaaataa taaattattc
    10141 aatagaaatt tatttgataa tataagattc ccactgggta aattaatgga agataaggct
    10201 actacttatt tgctgatcga tcattccgaa aaagttgctg tcaattcatc tgctaaatat
    10261 cactattacc aaagccctaa aagtatactg agaggaagct tcaatcctag aatttttgat
    10321 tcttttgaag tccatgaaga tatactaata ttcatagata agttttatcc ggaattatca
    10381 attaatgtta gaactcgcta tgtctatgaa gcaataagga tgatgatgac aatgattaaa
    10441 aataactata ccaataaaga agattattta cgttctataa atattttaca acgctttgaa
    10501 aaagaagtga aaattgatcc cgacttttca ccaaaattaa aaaaattagt aaagttttta
    10561 ttaataaata aaaacttgcc ttactatatg tcaagtagta agatagcaag taaattcttg
    10621 attaagatag aatctgtgag gtaagagatg aagaatataa aaaaattttt ttcaaggaat
    10681 attttcccgg tttttattaa aatattacct attcaaaaaa atttaattgt attttcaagt
    10741 tttggcgggc gtaactattc tggaaatcca agagttattt cagagaaaat tcatgaaaaa
    10801 aaattgaatt acagaattat ttggttaatc aaaaaagaag cgattataac tgatttacca
    10861 gattatatta aaagaattga ctccgattct tttttagctg ccttttattt atatacagct
    10921 aagtattgga tagacgatag tcggaaaacg attaagtatg caaaaaggaa aaaacaactt
    10981 tattttcaaa catggcacgg aacgccttta aaaaaaattg aatttgatgc aaaagataag
    11041 ttacctaaac gttacctaag ctatgcgaaa aaagatagtg agtcaattac atatttatta
    11101 tctggtaatc gctatagttc taagaagtat gtttcagcat tcaatatcgt tccttctaaa
    11161 atattagaag taggaactcc tagaaacgat gaattagcct ctagtaaaga gcaagtgttt
    11221 gacttgaaaa agaaacaaat caatgtctta tttgctccaa cttttcgaaa taatattgag
    11281 gacaacggaa ttacgcaact agaatggtta ggtgtggata acttacgtga attttttaaa
    11341 aagcaacaac aagaattaaa ttttatgact aaatttcatc ctaatgttca tagtaaatta
    11401 gcaactgatt ctaaaagtat agaatatatg aaaaaacatc aaattacttt aattaatgat
    11461 gggattcctc ttgaaatgct ttttgaagag atagatgttt taatcacaga ttattcatct
    11521 atttttttcg attttgcgct aacaaaaaaa ccaattattc tttttaacta tgatgaaaaa
    11581 gaatatagct tagaaagagg tttttatata cagttaagtg agttaccaat tataagcgta
    11641 caagatagtt atggccttgt tgatgttttt aaaaaccaac aagaaaagct tttgaaaagt
    11701 tctcaagagt tacttgatta tattggaaat tatgaaacag gagcaagtac agaaaaggtt
    11761 cttgagttga tagagaggga tataaagtga aaaaaacagt aaaagatata ttttataatg
    11821 cgatatatca agtctttttg attgttttac ccttacttac tatacctata cttagtcgta
    11881 gaattggttc aactggttta gggatttatg ggtatgtttt ctcaatatca cagtttttaa
    11941 tgactgtcat tgctgtggga atgaatcctt ttcgtattcg aaatatcgct aaatcaagga
    12001 aagataaaaa agcactatct ttacaattct ggaatattta ctttattcaa tttttaatag
    12061 gcttgagtgt ttcctttctc tatatagcct atatttattt ttttgatgta aagtataaaa
    12121 atttatactt aattcaattg atatttattg tagggctaac attagatatt tcttggtttt
    12181 ttcaaggaat tgaagaattt tcgaaagtgg ttattagaaa tacagttatc aaattatcat
    12241 cggtattgtt aataatcatt tttgtttcaa ctaaagatga tttgtggatt tacgtgttca
    12301 ttacttcaat tactaacttt ttaggaagtt tagttttctg gttaagtatc cacggaaaag
    12361 taggtaaacc aatctttaac tctaatgttt ttaaacagtt gtggaaacca gcttttatta
    12421 ttttgactcc tcagttgttt atgcaagtgt acacaacttt agacaaaacg atagttgggt
    12481 attttgtgga tccaacggaa ttatcttatt atgatcagtc tcaaaaaatt gctcgtatta
    12541 tattggcagt cttaacatca attactattg ttatgttgcc taaaatatct aaagcacagg
    12601 cagaggggaa atcagaaaat attttagttt acacaaaaaa atcttttgat tacacgatta
    12661 tcattgcatt aattcttttt agtgtggtgt ttatcaatac aaaagaattt gttccatggt
    12721 tttttgggga gaatttcaaa ccgatgacaa taaacatgct tatttcttgt ctaattatag
    12781 ttgttagtcc aattggtgga gtttttgcta accaatttgc aattgctata gaaaaagata
    12841 aggagtatgc ttatccaatg atcattggtg cgattgtaag tattattgga aatgtagctt
    12901 ttgtgcctac ttatggcgct gtagcggcta cgatcgtact cgtggtagta gaattattag
    12961 tctttttctt tagagtattt tttgtacgaa aagaaataaa aatatcctat ttctttggga
    13021 agaacgttat cttatttttt atagtcaatc ttactttgac tacttgggcc tattactttc
    13081 ttccacaatt attctcttct tcatttttag atatggcaat taaatcaata atgattttca
    13141 ttatttatct tccgtttgtt tattttcttt ttccagatat acgaaatgaa ataagaaaga
    13201 ttactcgaaa ataaaataat gttgtaaaaa agtagttctt tgtcaaatcg ggctgataag
    13261 gtcatttatg aacaacaaag ttagtaagag ttactagctt ttaatgcata gctgattaac
    13321 ctgttgatta gaaaaatcca caagcgcata ttggtaaaac tgcgaaaccc ataagcaaat
    13381 cgtttcaatg ttttgatatg tgtgcgaaga ttctctaatt ttgttattag aatacttttt
    13441 cgaaaatgct agttaaaagc ctttttcaga tatgaaaaag gctttttgag atagtgaagt
    13501 ttcgagggaa cttctttggt cgtttctcga agtagctagc tgtttcttgt gataattgct
    13561 aagcagcttt tgataaactg cttagcaatt ttaagttcat aagagattga gggtagacga
    13621 tcaactactt cactttctgt taaatagcgc cattgaaaat tctgccattt cttgaatgtt
    13681 gtgtaatcca actcgtcatc tgattttaga agtaatttat acaaaggttt caattgtcga
    13741 tagtacttag cttgtgaagt gtctttttga ttcaactgct tcatcacacg ttttcgaaca
    13801 cgatcgaaaa acttgttcaa gtgttgtcca atgtgaaagc aatcaatgat gatttgggcg
    13861 tttggaaagg actctttgac taatgaaaaa taaggggcat tcatatttgt gacaatgtat
    13921 cgaaccccca tatgagcttt tcttgtaaaa cgcatgaaat aagcaatcaa atcttttttt
    13981 ctccggctgg acaaaatgtc aaagagcttc cctgtctctc tattcataca agaaaaggag
    14041 aactttccat gggaaattga aagagagcga aactcgtcga atagtaaaca atgaggtaaa
    14101 tagtctagca tctgtcgcag cattgccgat agagcagata atgtccgttg aaatgtcgtt
    14161 actgaaagat cacaacgttt cgtaagtgtc tttaacgctt gacgctcggt caactcttct
    14221 aatatcgtgt aatgaacttg tttcgaaata aaacatgaag gaacgacaag atacgtatga
    14281 gcagtaaaat attttccgca tgattggtag tggtaatgtt gttttctaag acgcaaatag
    14341 gtttatttat tcccactgcg atttaagata atcattgaaa tcttcttgcc gtttttcacg
    14401 agctgcttcg cagtttcttt acaaaataga caatgtctaa aagtgcgaac taatgtacct
    14461 tcgattacga tggctttctt gtccttataa aaagtttttt gaaaagaatt aatttcaata
    14521 tttttatctt taatagtgaa gtgctctttg atagaacgag aaatggatat acaaaatctt
    14581 cctttcagat tgatgtttta gacgactcta ttttatcgaa atttttgtat gtctgttttt
    14641 tttacaagaa aatctaggat cgacagaaaa ttctatcagt cctagaaatt atagagccat
    14701 ttttttacgt cttatccaga tatattgttc tttcattgtt taaaaaatat tatttatcac
    14761 tatttaaaga attaagttcg ttctttattt gagttgtaga gatctcaggt gttcttggta
    14821 aataaattac ttgtgcatct gttagttgtt caagttcatc aaattgtcca gcccaatcat
    14881 cgcccatcac gaaataatct gcatggtatt cttttatatc ttttactttt tgttcccaat
    14941 tttcttcagg aataactaaa tcaacgtagc gaatagcttc aagtaactgt tttctttttt
    15001 cataactaaa ataacatttt ttttgtttcg cattccaatt gaattcatct gtagaaaggg
    15061 caacaataag ataatctcca tattgttttg ctcgtttcag taaattgata tgcccgtagt
    15121 gaagcaaatc aaaggtaccg taagtaatta ctcttttcat ctttcacctc tcgaaattca
    15181 ttaatataca ttgttagttt agcatttttt tgttagaatt gaaagaacca gtttatccta
    15241 aaaaggataa gaaatgttta tgagaaatag gtggatggag gaaaaatgtt gaatgaaaaa
    15301 tttttaaaat atgtgaaagg ttggtatttt atttattttg gttttattct aatgttacga
    15361 gcaataggcc cgtatttact attacctact agatatgata ctttactttt ttggcctgct
    15421 ggaatagcgg gagttgtctt gatcattata gatgcaatta ttgcatttag agaaaaaaat
    15481 cttaaaaaat atgattggct attaattgct tttattggcg cgatgctagt aagcagttta
    15541 atgaatttaa aatatggata tagcgaaaat atcaagttaa tcatgtggca attaatcttt
    15601 ttctttatga tttatcagtt tggcaaagac tacagtagta actttttttt caaagtattt
    15661 acttggatac tttcattggt atggttttta gctaatttag tatcattata ctggtttgtc
    15721 attcaatatg gcataaaaat tccaataaaa tataagtatt acccagttcg tttagggtgg
    15781 ttaggcaacc gtttatttgg tgtatataca gatccaaatt ttggtgcggt tatatcatta
    15841 attgtcatca ttattgctat atattatttg gtcacttaca agcatttaaa tcttctattt
    15901 aaaatatttt ttgcaattaa tatttttatt caattctcgt ttattgcttt atcgggctca
    15961 cgcaccacat tagtttgttt attaatagca acttttgttt ttagtttctt tacaagttat
    16021 agtagtcgtt ttcttaaaac taatacttta aaaagatggc taactgcatt agtagtagga
    16081 ttgctaagta taattgcgat gtattatgta acagatataa ctgctcaagc atgggcacat
    16141 gttccgcata ttacagaaat aaaaaaagat atatctgatt catcaggaac aactagcagc
    16201 aataaagttg atttaaaacg ccctgacact gatggcgggg atgtttctaa tttaaggttt
    16261 ggactttgga aaagtgcaat tgaaatattc aagagtaagc cgatatccgg cggttctcca
    16321 ggatactata ttgaatacgc acatgataaa ctaccatata cattaatggg aaaagatact
    16381 ttaactgctc ataattttat tttcttagtt atggctgcta caggaggatt aggtctattg
    16441 acattgttaa ccttcttatt tatccaatgc tttaaaactt tgcgttattg tttcacacaa
    16501 ccaaatctag taaaagataa gcactttttt gctattttaa tgattcttac aatagcagtt
    16561 tcggcattat ttatcacaga actcattttg gttagtacga taggttcatt tattttttgg
    16621 ggatatctgg gttctatcca aaaaactata tctaaagaag aaggagtaac taattgaaac
    16681 tatttttagg tatataaagt agaaaagaaa actagttatc ccaacttcaa caaattttat
    16741 agtttttaca atataaaaat ggtaaactaa ctttattaag tatgagaatg cataattgga
    16801 gtgaatttga tggatactat attgattact ggtggagctg gttttatcgg ttcaacgcta
    16861 gcgaattatt tgggaaaaga aaacaaaatc gtagtagtcg acgatttatc aatgggaaaa
    16921 aaagaaaact tggatatgga gaaacaagtg actttcattg agggggatgt atcagatcct
    16981 caattgatgg aaagaatcat gaaacagtat cagtttgcct atatttttca tttagctgct
    17041 gtagctagtg tggcagattc tgtagaacgt cctctagaaa ctcatcgtgt aaattttgac
    17101 agtgcgcttt tattgcttga attggtacga aaatatcaat ctggtttaaa acgattggta
    17161 tttagttcat cagctgcagt ttatggcgat gaaccaacgt tgccaaaaaa agaagaatcg
    17221 gtcatccgtc ctttaacacc atatgccatc gataagtttg catcagaaca atatgttttg
    17281 gattactgtc atttatatga tgttccgact agtgctgtcc gattttttaa cgtttatggt
    17341 ccaaatcaaa atcctaactc tccatatagc ggagttattt cgattctagt ggaccgatac
    17401 aaaaaacaat tggcaggaga aaaaacagaa tttactttat ttggtgatgg tagccaatct
    17461 agagacttcg tttatataga agatgttata caagcacttt tacttgttgc taaggaagaa
    17521 aaagctttag gacagcaatt caatgtcggt acaggaaaaa gcactacgct acttgaattg
    17581 attcattcaa ttgatcaaat cttaggaaca gagctagcgc ttaaatatga agcggagcgt
    17641 tcaggggata ttcgtgattc tttagcggat atttcaaaga tccgttcttt aggttatcaa
    17701 cccaaatttg atatattgaa cggaatggaa cgttatttaa agacagaaat cgattgaaac
    17761 aaaaaaccag catcctgctg gtttttttgt ctcgacttag ctttttaaat taacaccttc
    17821 catcagttcg taaatctgat ttcttaaatc agtggccggc aatttgatgt ttttatcaga
    17881 ttggatatta ctttccatga cgattgcatc ttttgtgtct tttttcatcg ccgctactgc
    17941 atggaatcga ttgatatttc cctgagcaag gatataatca tttccactgt aaacaccgcc
    18001 gctgtaagga tgtttatcac cattattttg ccacaatgag aaaagtagtt cttttggtat
    18061 aatatcgcca gacagaacac tggtaaagaa tttgttcaag tcataaacag acatacttat
    18121 atttcctgtg cctaattcat ttcggatgtc tgtttcaggc tcgaccaatg cttttgaata
    18181 atcgtcagtt gcagacattt ggtaagatac agcatgctga ggggaatttt ttacattttc
    18241 ataaaaatct gtatgttcta gatggagtgg ccgaatgatt tcttttttta aaagagtttc
    18301 ataaggctga ttcattagtt tgatcaatac ccctgtaagc aatgtataat ttaatggttc
    18361 ataatgatac gtatcataac cagtaaattt caaatgatga agggcaaact gaatgatctc
    18421 actatctgac ataggttttt ttggagaagc ggttcgatga agaccagagc gcatgtaaag
    18481 catatttttg attgagattt gctgacttcc aggaatctga ggataaaatt cagatagctt
    18541 tgtgtcaaaa gttatcttgc cagattgaat ttgttttagg ataagtaaag cagtcatcgc
    18601 tttttggatc gaaccgattt gatagtaaag agaaggagaa ttcgattctt ttatgcgtag
    18661 atttgcgtac ccatatcctt tttctaatat cactcgatta tttttgcgta cataaatcga
    18721 accgatataa tctgattttt gcagtaattg atcaattttg ctggcaagca tctgctcatt
    18781 agatgttaaa ttggaagcaa gttgtgcggg ttccgtttta taaatttgtg gttgaggttg
    18841 ttttgttaat tgccatcctg taatgatgcc tgttgaaaag aagctgacag ccagcaacaa
    18901 aactgcgaga aaagaaagag agatctgctg ttttttttga tgttttcctt ttgccatatt
    18961 aaatcctttc tatccatttt taactatttt tcattgtaat cctcaaaaag agcatttgcc
    19021 acttacaatt ttgacataat atttggtaaa ataattcacg gcagtagaaa tttcatgtat
    19081 aataaacaga gttacgaaga tgatagtaag gaggagttgt tttgtcagga aacaaacaac
    19141 acccaagcaa agacaaaaaa gaagccagta aggcagctac agcagatata atcgacaagc
    19201 ggagaaatct gcgtaaaaaa gaagataaga tcgtgaggaa gatcatttta gtgatcgctc
    19261 ttacattatt gattattggc ggatttctcg ggtttaccgt atatcgttat gtagatagcg
    19321 ggttgaagcc tttagataaa tcggacgacc aattagtcca agtggaaatc ccaaacggct
    19381 cttctaataa gcagattggc gaaattttgg agaaagacaa tattattaaa agtgggattg
    19441 ttttcaatta ttatacaaaa tttaaaaact taactggctt tcaagctggt tattatcagt
    19501 tagcgcctaa tatgactctt gatgagattg gcaagcagtt gcaagaaggc ggcacatctg
    19561 agccaacaaa agttgcagat gggaaaattg ccattccaga aggctacgac atcgatcaga
    19621 ttgctgagcg ggtagcgaag gtaacaggta aagacaagaa agaattttta gatttagtaa
    19681 atgatgagac tttctttaac gagttgtatc aaaaattccc agaattgttg gaaagtgcct
    19741 ctaaagctga gaacacaaaa taccgtttgg aaggctactt attcccagct acttatgatt
    19801 actacacgag tacttcgctt aaggatcttg tgattgagat ggtgaacaag acaaatacgg
    19861 tcatgcaaaa ctattattca gctatcaaac agaaaaattt gactgtacaa gaagttttaa
    19921 ctttggcttc tctagttgaa aaagaaggcg taaaagaaaa tgatcgtaaa aatattgcac
    19981 aagtcttctt taaccgaatc aaggcaaata tgccacttca gtcagatatc tctgttttat
    20041 atgcattagg agaacataaa gaattggtga cgtatgaaga tacagctgta gattcaccat
    20101 acaatttgta taccaatacc ggctatgggc ctggtccttt tgacaatcca agcgaaaagg
    20161 caatcaaggc agtattagaa ccagcagaaa atgactatta ttatttcgta gcagatacga
    20221 gcactggaaa tgtttatttc gctaaaacgt atgaagaaca tatggaacta gtgcaaaaat
    20281 atgtaaataa ttcttagaaa aacaggggct agtcattgat catttttaga aaaacattta
    20341 cattttaaga aaagcgtggt aatctttttt ggactatatt cctcaaaaga ttaccttttg
    20401 cggtatttta tcgaaaaaag gagacgttac acatggtaga gaaagtttat ccaatgactc
    20461 tagaaggtaa agaaaaatta gaaaaagaat tagaagaact aaaaacagtt aaacgcggag
    20521 aaatcattga acgaatcaaa atcgctcgcg gtttcggaga tctttccgaa aactccgaat
    20581 acgaatcagc taaagatgaa caagcatttg ttgaaggaag aatcacaaca attgaaaata
    20641 tgatccgttt tgcacaaatc atcgataatg acggcgtaga tgcagatgag gtatcgatcg
    20701 gtaaaacagt tacgttcatc gagttgcctg atggagatga agaagagtac acgattgtcg
    20761 gaagtgctga agcagacccg ttttctggaa agatttccaa tgattctcct atcgcgcaag
    20821 cattgattgg caaacgtaaa aatgatgaag taaccattgc tacaccaggt ggcgatatgt
    20881 tagttaaaat cgtcaaagta gaacaagcgt agtctgactg aaagatctag agagttcatc
    20941 ttactgatga cgcactctag atcttttctc tttgagtatt gcaggtttca ctgaacgatc
    21001 ttacatctaa agtcctatag ccaacagtca aaaaaatagg tatgatgaaa tgagaatgtg
    21061 tcaggaggga gaaaaagcga tgaatagttc ttggcgtttt tttgtggtag tcgaagcact
    21121 gctgctgtta tttgctgttt ggcaaatcgt aaacaatact ggacttttgc ttttagtaat
    21181 attcggtatt ttcaatatat atctagctat gcgcaaatat cctcgtacta aatttcaaaa
    21241 ttttcaattg atcttaggga gcttggtcat tttttttagc ttagtcaaca gtcctgcttt
    21301 atggatgatg gttgtattgg ccgttttatt tatcgggctg aaaggtgtag agatttcagg
    21361 aatagatttg acaaaaaatg ctttttggcg aaaaaaacag atcatgatgg tccaaacaga
    21421 gcaattaaaa acacataata atgagcgtaa aagacagcaa ttatttggta accaacgtat
    21481 tggaaacgac gtctatgaat gggatgatat caacattgcc atcatttctg gtgatactat
    21541 catcgatttg ggtaatacac ttttgccaaa agatgataat attgtgattg taagaaaagg
    21601 gataggcaga acacggatct tagtacccct gggagtggct attcgtttag agcatgcgac
    21661 attagtagga aatgtcttgt ttgaagaaga gcagttttcc ttaaaaaacg aacagatcaa
    21721 aatctgcagc agtgattatg atgaaaatcc tcgcagattg aagatcatta ccaatacctt
    21781 ggttggagat gtcgaggtga tacgaatatg atgggaagaa tatccaaagc aatgctagct
    21841 gtttattcgg ggattgcggc tttccttatt attctatttt cacttttcac ttatttttat
    21901 gccagtaacc aaagtcattg gtggggagaa ttgctgcgtg cgcgtttatt atacgttccg
    21961 cttatcttcc atttgatggc aatttcatta ggtgtaggat tgatcgtatt tctattgtta
    22021 tcacttattc aaaaggcaaa atacgggaaa atcgaagaaa agcttcgtgc actttcttct
    22081 ggcaattatg aatccaaact tttgcatcag ccgatcccaa gcgcgtcgga tgatctgtac
    22141 atcaaagata ttgatatgga aattgctaaa ataaaagaaa aaatgatcga agtatccagt
    22201 gaattacaga ttatgactag ccgtcctcaa tatgtagatg gacaaaccaa agaagagatt
    22261 ttagaattag aaaggcatcg attagctcgt gagcttcatg attcagtttc acagcaatta
    22321 tttgcagcga tgatgatgat gtcggcattg acggagcaag cagaaaaaag tgagacacca
    22381 gaaatgttcc gtaagcagtt gaaaatggtg gcagagatca tcaacgcttc ccagtctgag
    22441 atgcgtgcgc tgcttcttca tctgcgtcca gtcaatttag aagaaaaaag tttaaaacaa
    22501 ggcattgagc agttattgaa ggaattgcag aataaaatcc agatttcgct aaaatgggat
    22561 gtagaagatg taaaattaac tagttccatt gaggatcact tgttccgaat cgttcaagaa
    22621 ttgttatcaa acacattaag acatgctaag gctaatgaat tagaagtcta tttgcataaa
    22681 atagataaca atcttttatt acgtatcatt gacgacggga caggatttaa tatgaatgaa
    22741 accaaaacgg gaagttatgg attgaacaat atcaaagaac gagtagctgg tattggtgga
    22801 acagtaaaaa tcattagttt taaagggcaa ggtacgagcg tggagatcaa ggtccctttg
    22861 atgaaggagg catagataaa gatgataaaa gttttattag tagatgacca tgaaatggta
    22921 cgcttaggcg tctcttctta tttatctatc caagaagaca ttgaagtgat tggagaagct
    22981 gaaaatggac gtcaaggcta tgaaaaggcg atggcacttc gaccagatgt cattttaatg
    23041 gatctagtca tggaagaaat ggatggcatc gaatcaacaa aagcaatctt aaaagattgt
    23101 ccaaaagcca aaatcattat cgtcacaagt tttattgatg atgaaaaagt ttatcctgca
    23161 attgaagcag gagcagcagg atatttgttg aagacgtcta ccgcacatga aatagctgat
    23221 gctataaggg ctacgcaacg cggcgagcga gtattggaac ctgaagtgac aacaaaaatg
    23281 atggaaaaaa tgagtcggcg aaatgatcca gtattgcatg aagaattaac gaatcgagaa
    23341 aatgaaattt tgatgctgat ttctgaaggg aaaagtaatc aggaaatcgc ggatgaattg
    23401 tttatcacac tgaaaacagt taaaacgcat gtgtcgaata tattagctaa gttagaggta
    23461 gaagaccgaa ctcaagcagc tatctatgcg tttaaacatg gtttggtgaa ataaaaacga
    23521 tgataaagag gataatcaaa agctgagaaa ataactatta tttttcttgg cttttttaca
    23581 ataatgatgc ttgcttttgt ttttggaaag gctttgttat actgagaaaa gaagatacta
    23641 aaggaggaaa atggtgaaac aaaattttgc aattgtaggc cttggacgat ttggcggaag
    23701 catctgccgt acactgatcg aagctggaca agaagtactg gctattgaca gtaatgaaga
    23761 tcgagtcaat gaatatatga atatagccac tcatgctgtt gtggcaaatg cacaggatga
    23821 gatgacactt cgttcattag gtataagaaa tttcgatcat gtaattgtcg ctattggaga
    23881 agatatccaa gccagtatct tagttactct gatggtaaag gaaatgggcg taccgaatat
    23941 tttagcaaaa gcccaaaatg agtaccatgc ccgtgtacta gagaagatcg gagcagatcg
    24001 tgtcgtacac cctgagcgag acatggggat acggattgca cataacttag tatcaaagaa
    24061 tattctagac tacttagagc tgtcagataa atattcttta gcagaaatcc gagtctccaa
    24121 tcctaaattt ttcaacaaaa cattagctga attaaatttc aggcaacgtt ttgacttaac
    24181 agtagtcgcc atacgaagag tagatcaaac ggttatcgct tctcctgcag cagatgaata
    24241 tgttcgcgaa aatgataatc tactggtgat tggagagaca ggcgatgtgg atattctaga
    24301 cgataagatg aatcaataga aagaaaagag aaagtaggtt gttgggatgg aattaacggt
    24361 aacgccaaaa gcacaaaagt ggtttgactc agaaattgat ctgccagaaa cttacggaat
    24421 acgttttttt ggaaaagtct acggcaaaac agaggttcat gatggttttt cgattggaat
    24481 gtcagttgaa cagccagaac atcctgtaaa gaaagtggaa atggatggga cacttttttt
    24541 tatcgaggaa gcagatgaat ggttctttaa aggatatgat ttacttgttg attatgatcc
    24601 caacctggag gagccaacct atcactttaa aaaacaataa aaaaagagag ggtgacacaa
    24661 gttt
//
LOCUS       DACKAC010000043        24436 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.043, whole genome shotgun sequence.
ACCESSION   DACKAC010000043 DACKAC010000000
VERSION     DACKAC010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.043"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(368..1486)
                     /locus_tag="I0B46_001748"
     CDS             complement(368..1486)
                     /locus_tag="I0B46_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084051.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1957..3264
                     /locus_tag="I0B46_001749"
     CDS             1957..3264
                     /locus_tag="I0B46_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAR2084052.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3383..4582
                     /locus_tag="I0B46_001750"
     CDS             3383..4582
                     /locus_tag="I0B46_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAR2084053.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4609..5877)
                     /locus_tag="I0B46_001751"
     CDS             complement(4609..5877)
                     /locus_tag="I0B46_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAR2084054.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(6040..6686)
                     /locus_tag="I0B46_001752"
                     /pseudo
     CDS             complement(6040..6686)
                     /locus_tag="I0B46_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     gene            complement(7094..7597)
                     /locus_tag="I0B46_001753"
     CDS             complement(7094..7597)
                     /locus_tag="I0B46_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAR2084055.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(7720..8484)
                     /locus_tag="I0B46_001754"
     CDS             complement(7720..8484)
                     /locus_tag="I0B46_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAR2084056.1"
                     /translation="MKEIQSVKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLAEVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSSTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            8591..8866
                     /locus_tag="I0B46_001755"
     CDS             8591..8866
                     /locus_tag="I0B46_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAR2084057.1"
                     /translation="MRKVKMNVQGRVQGVRFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            9582..10535
                     /gene="yidC"
                     /locus_tag="I0B46_001756"
     CDS             9582..10535
                     /gene="yidC"
                     /locus_tag="I0B46_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAR2084058.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGGAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVIMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAAKPADNKQATKQIKQPKNSTNNKGRNAGRQQKRK"
     gene            10657..11866
                     /locus_tag="I0B46_001757"
                     /pseudo
     CDS             10657..11866
                     /locus_tag="I0B46_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017466553.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            complement(12176..12397)
                     /locus_tag="I0B46_001758"
     CDS             complement(12176..12397)
                     /locus_tag="I0B46_001758"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAR2084059.1"
                     /translation="MFKNYTINQKILPLDMEQYISETDVAFAVNALVEEKIKVDLYVS
                     SKFTNVKVVGADLFVVNVRKQKVSETDKF"
     gene            complement(12558..13079)
                     /gene="tnpA"
                     /locus_tag="I0B46_001759"
                     /pseudo
     CDS             complement(12558..13079)
                     /gene="tnpA"
                     /locus_tag="I0B46_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631987.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(13170..13536)
                     /locus_tag="I0B46_001760"
                     /pseudo
     CDS             complement(13170..13536)
                     /locus_tag="I0B46_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875519.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
     gene            complement(13550..13835)
                     /locus_tag="I0B46_001761"
                     /pseudo
     CDS             complement(13550..13835)
                     /locus_tag="I0B46_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
     gene            complement(13841..14035)
                     /locus_tag="I0B46_001762"
     CDS             complement(13841..14035)
                     /locus_tag="I0B46_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084060.1"
                     /translation="MAETRPKGYPKLKEYTPSRFMLSECHYDKARADRAVNFIGQLRH
                     TKGKWAGNRFWLLPWQEQII"
     gene            complement(14035..14511)
                     /locus_tag="I0B46_001763"
                     /pseudo
     CDS             complement(14035..14511)
                     /locus_tag="I0B46_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004070864.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
     gene            complement(14979..15332)
                     /locus_tag="I0B46_001764"
     CDS             complement(14979..15332)
                     /locus_tag="I0B46_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084061.1"
                     /translation="MAMSEKAKKYLKEIKGAKTIPELKDVEIAIKRDGILAWAEFTKL
                     NEAVEEKKVALRKKKQETSLQEILFWAYKKESDKLLKMMDEGADKDAIQMQVQRYDSI
                     GQIIAEADLEDEYEV"
     gene            complement(15460..15999)
                     /locus_tag="I0B46_001765"
     CDS             complement(15460..15999)
                     /locus_tag="I0B46_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084062.1"
                     /translation="MQLLLYRIGDSTKIRFLINVRFRVIYSTVLPKGIPQRQKWMIHM
                     EEIMQMILGNAKFQLGQVVSTPAVLETFNPLQTANGIPEALSNLWFRQEISIAEIEDA
                     VLDAKNGKELANSLNRIKLFRKFTLDRELDGMVRLKGTDVWKNISYLKVYTATPPKKP
                     TPPRFQSNGWSLFFSHKSA"
     gene            16029..16220
                     /locus_tag="I0B46_001766"
     CDS             16029..16220
                     /locus_tag="I0B46_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084063.1"
                     /translation="MIIRDDHIYTCDSCHYSFPADEQPERCPDCEKTATRLDTEIETE
                     DYYRVRAEIKAEIKALNAG"
     gene            complement(16262..17128)
                     /locus_tag="I0B46_001767"
     CDS             complement(16262..17128)
                     /locus_tag="I0B46_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAR2084064.1"
                     /translation="MEELFSNALKSVKSNRVLYTASFFARSSLLYLQEIGQLTALKAH
                     TSRRTGLSSYLCFLVEVGSGTLEYKGQKYTLQVGDMVFIDCLKPYVHESSEDDLWTLR
                     WCHFNGIGMTEVYKKYLERGGQPVFKTSKTDKYADLLRSLHMVAEGQEYVRDMKINVL
                     LSELLMHLMEDSWNPDKVPNFSTKKMELQNIKQYLDDHYTEKITLDQLSAEFFINKQY
                     LAKIFRETYGTTVVGYLNQKRITQAKHLLRFTNLSMEEIATKIGITDANYFSRTFFKV
                     EGVRPSEFRKMW"
     gene            complement(17152..18804)
                     /locus_tag="I0B46_001768"
     CDS             complement(17152..18804)
                     /locus_tag="I0B46_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAR2084065.1"
                     /translation="MNYKVEYERWLNHANADIIEELRSYSEKDIEDSFYKNLSFGTGG
                     LRGTIGAGTNRMNVHTVGKASQGLADYLNKNYEAASVVVGYDSRIKSDVFAKTAADIF
                     AANGIQVHLWPELLPVPTVSFATRYLKADAGVMITASHNPSKYNGYKVYGADGCQITT
                     EAATAILAEIEKLDIFADVKMDGDASKIASIQSEVLTAFIEEVKGQSVLFGEEVDKNV
                     AIVYSPLNGTGLVPVTRTLAEMGYSNVTVVEEQRLPDGNFPTCPYPNPEIKEAMSLGM
                     EYAKKYNADLLLATDPDCDRVGIAVKSAHGDYVLLSGNETGLLLLDYICSQRIKHGKM
                     PADPVMVKTIVTMDLGERIASNYGVRTIDVLTGFKFIGEQIGMLEKAGKVDSYIFGFE
                     ESYGYLSGSYVRDKDGVNAAYLICEMFSYYKTQGISLLDKLNEIYTKYGYCLNTLHSF
                     EFDGAAGFTKMQEIMVEFHKGLDIIGGKKVIETLDYSKGLNGLPKSDVLKFMLEDNCS
                     VVVRPSGTEPKLKTYISVSAENKEAAEAMEAIMAAELAEMFE"
     gene            complement(18809..19711)
                     /locus_tag="I0B46_001769"
     CDS             complement(18809..19711)
                     /locus_tag="I0B46_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HAR2084066.1"
                     /translation="MMNLQKELRCLLLRKLGMLYAPGWTELCCVWSEYQGMTLTEVLQ
                     EHPEFLGTHPQTKGELPILIKFIDAKKDLSVQVHPTDEYAIEKENGQLGKTEMWYVLD
                     ASLIYGLQKITEEAVIREAIAQGTVERYLQKVPVKKNDVFFIEAGTIHAIGAGALIAE
                     IQENSNLTYRLYDYDRVDKNGKKRELHVDKALEVANLKGLSEPKQPLRVLKYRAGVAA
                     ELLCRCKHFEVSRMIVNTERRQTVHYRADSISFRVLLCVNGCGTVRFDNESLDIYKGD
                     CLFVPADSTEISIHGSIQFLDVRG"
     gene            complement(19795..21108)
                     /locus_tag="I0B46_001770"
     CDS             complement(19795..21108)
                     /locus_tag="I0B46_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAR2084067.1"
                     /translation="MNIVLLSGGSGKRLWPLSNDIRSKQFIKIFKTEDGYESMVQRVY
                     RQIKKVDNDATVTIATSKTQVSAIHNQLGEDVGVSVEPCRRDTFPAIALATAYLVDVM
                     GIDPEESVVVCPVDPYVEDDYFEALKSLSDQADKGEANLVLMGIEPTYPSEKYGYIIP
                     ESAEQTAFVNTFKEKPSEAVAKEYIAQGALWNGGVFVYKLKYVMERAHQLIDFVDYQD
                     LFNKYDTLTKISFDYAVVEHEDKIQVQRFAGMWKDLGTWNTLTEGMEESIIGKGVMNE
                     NCDGVHILNELDVPVLAMGLHDVVICASREGILVSDKEQSSYIKPFVDKFEQQIMFAE
                     KSWGSYKVMDVEAESMTIKVTLNASHSMNYHSHKNRDEVWVVLAGTGKTIVDGYEQVV
                     KAGDVITMSAGCRHTIMADTELKLIEVQLGKDINVKDKQKFELEY"
     gene            complement(21176..22120)
                     /locus_tag="I0B46_001771"
     CDS             complement(21176..22120)
                     /locus_tag="I0B46_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HAR2084068.1"
                     /translation="MMEKDAKIYVAGHRGMAGSAIVRELNRQDYNNIITRTHKELDLC
                     RQDAVEAFFAQEKPDYVFLAAAKVGGIIANQNALADFMYENMILEMNVINSAWENGCK
                     KLQFLGSSCIYPRMAPQPMPESCLLTSELEKTNEAYALAKISGLKYCEFLNKQYGTDY
                     ISVMPTNLYGPNDNYHPTHSHVLPALIRRFHEAKEAGLPTVTCWGDGSPLREFLYVDD
                     LANLCVFLMNNYSGDETVNAGTGKELSIKELTEMVAKVIGYEGEILWDTSKPNGTPRK
                     LLDVSKATKLGWTYKTELEDGIRLSYEDFLNNPMRAER"
     gene            complement(22157..23170)
                     /locus_tag="I0B46_001772"
     CDS             complement(22157..23170)
                     /locus_tag="I0B46_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084069.1"
                     /translation="MATLCRCEAKITLVCGANHLDFAKRNLEQELPKELIDRVRYRPI
                     NTDIGLINREGTLLVDTGKLTEICEYFLAKVPSLAKTEAEWLKREAVSAVLCDMPIWA
                     MDACDMAGVPFLYVGNFTWTELYREFLPEHIWKEYAKRYGKIQHTMLYALNNEEMLEF
                     LQNSEKTPTSVVARPIHNDEVQRIKSQHEKPPVFVALGMSARFKQPIDVSHAPYDFIT
                     TLGVPLVGENVTVISNSTPNTQDYIAAADYVITKAGWGTVAECILAQRPMALFARDTV
                     LEDRTTICKLQKLGLAISVTPENLHRVERVIEDLKQLDTSNFDTFYDAASEIADKLIS
                     LMQ"
     gene            complement(23250..24296)
                     /gene="gmd"
                     /locus_tag="I0B46_001773"
     CDS             complement(23250..24296)
                     /gene="gmd"
                     /locus_tag="I0B46_001773"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HAR2084070.1"
                     /translation="MKKALITGITGQDGSYLADFLLEKGYEVHGITRRASISNTARID
                     HLMGKITLHDGDLSDSSSLIRIISIVQPDEIYNLAAQSHVQVSFDVPEYSGDVDALGV
                     MRILEACRILGLTKKTKIYQASTSELYGKVEEVPQRETTPFHPYSPYAVAKQYGFWIT
                     KEYREAYGMFAVNGILFNHESERRGENFVTRKITLAAGRIAEGLQDHLELGNMDSLRD
                     WGYAKDYVECMWMILQHETPEDFVIATGEQHTVRDFTEKAFAANGMTIRWEGTGIDEK
                     GYDVETGKMLVCVNPQWFRPTDVDNLWGDPTKAKTVLGWNPQKTSYEELVNIMAKHDR
                     ELAKREAAMKNAVK"
BASE COUNT         6950 a         5537 c         4651 g         7298 t
ORIGIN      
        1 tttttaaacg gtgttccaga agtagcttct tcggaaataa gccgaaattt cacaaaaatt
       61 tgagaaataa tttttgtcaa tctcttctta tttcttgaag ctgaccactt ctgtcacaac
      121 ctcttcctta aacggtgttc aaaagcttgc atctgcggta ataagcccac caaacaaaaa
      181 atatgaagag ctatttttgc ttgctgttct tattacttga tgcagaacag ctttttcaca
      241 acctctcata cacggtgttt aatcagctga ccttcggtat tagctcaaaa aacggaaaaa
      301 tatgtgaaac tatttttccg tttgtttctc taatactcag gtcaaaacac tgatttcaca
      361 acctctttta ataacgaaga atcgctgtca gactcttttc atctggcgca tctttttgat
      421 ccaaaataaa cactttcccg ccattaatga tcacatcatc tgctaatgca ttcaacactt
      481 gacgccgatc atattctgtt tcaggatctt caccgaaacc atcagctaag tttgacgtag
      541 cgataaataa atgtgacact tttccttctt tcgcagctgg aacgatatca actaactgat
      601 cgatgaattt cttatctaag agtttattgt attcttttga ttcttgagct gcgagttcac
      661 ttgacagctt cttcgcccct tcttcgatat ctctaaagga tagctgcgca ggagaagcag
      721 aaatcgcaac gtctgcacaa taatagggat ttttagcaat ttttttgaac atcgtctgat
      781 tttctggtaa tgcaaataaa taaatcggcc attgttcagg attttcgaaa gaatccttca
      841 agtacgtatc aacagcttgg taatagttga cccaatcgat ctctacttct tcatcttttg
      901 gatttacgcc atggaaagtt actccatctc gggctccgcc tcctccatta gaagagaagt
      961 tcaaaccgcc acctgtcagt tcagatccca atgcagtaac gacatcaacc ggtgcccctt
     1021 ctggtagaga tacttctttt acttgtttgt tgctgacctc atacaatttc atggagtcgc
     1081 ggttcaatcc caataaataa taagaatagt tgaactgccc atttttgata atagccagta
     1141 aataaggaag atcacttaca tagtattgat cgtctaccgg gatactcaaa cgatggatat
     1201 aggtctggtc agcagttata atgaccgcta cactcgtagt tgcactacgc caaaacgaag
     1261 gatctcctaa caaattttca attttttctt ggaacgggga ccagtcatgt tcggaatatt
     1321 ttttctcaaa acgtttcttt gcttctttcg caaaattttt gaatttgatc tgatcttttt
     1381 ccacatcttg atgggcgaca tgcgtattca gcatgatcgt gacgaatggt cctttagcgc
     1441 cttctgaagt caattgtgtc aataattctt tatccttttt tgccataaac gtacccctcc
     1501 taaaaaaatc attcctttgt ttctatctaa agtctaccac aacactattt ttccatacaa
     1561 taaaacgtat ctaaaaaaat aaaacggttt ctacggtttt aaatttagct atttttttaa
     1621 gttatcttca taatttcctc aattatttag ccccagtctt caactattct tcatgaaatg
     1681 ctgataaact aagaaaaaga ataaaagagg tggatcttat gcatttgatt atgattttca
     1741 gtttattaag tattatcagt ttatttgctt tcagcgcttc ccgcttatta gatgcggaaa
     1801 cacagcctat tcctgtaaaa gtacgtaaaa acgaccgcta aacttccata acgcgcgttc
     1861 ttatcctaaa gttttttcag aaacattgaa actctctccc ctctctgata aaattttaat
     1921 ttgtagcgaa acaaattaat tacaaggaga tcggatatgt ccataaaaaa aacacgtcta
     1981 gtcctattat tggctagtct cattctttgc cttgcttctt ttctaccagc tgttgctgtg
     2041 caagctgaag atacgttcaa agtaaatgca aaagcagctt ttgctgtaga tgcgcaaagc
     2101 ggtaagattc tttatgatca agatggagaa aaaaccatgg ggatcgcttc aatcacaaaa
     2161 atcatcggtc tttacatcgt ccttgatcaa gtaaaagaag gaaaactgtc atggggtgat
     2221 aaagtttcga tttcagatta tgcagaaaat ttgagtatca ccccagattt atcgaatgtt
     2281 cctttgcata aagaaaacac ctacactgtc aaagaattat tcgactctgc aatcatccag
     2341 tcagccaatg cttcaatggt ggccttggca gaaaaaatct ctggcagcga agctaaattc
     2401 actgaacgaa tgaaagaaca attgaaagat tggggaatca aagatgcaac aatcgttaat
     2461 gcatcaggat tgaacaactc ttatcttggc gaaaaccgac cagaaggcac tggagaaaac
     2521 gacgaaaatc aaatgtctgc tcaagatgtg gccatcgttg cccgccattt gatcttggat
     2581 ttcccagaaa tactggatgt atcaagtact acgacccaaa tgtttggcga aaatacgcaa
     2641 tcaccagtgg aaatggtcaa ctggaactgg atgctcccag gattcgttaa ttacaaggaa
     2701 ggcgtggacg gattaaaaac tggtacgact gagcttgcag gtgcttgttt cgttggtacg
     2761 atcactaaaa acgatcaacg gatcatcact gtcgtattga atgcggacgg ccatgcggaa
     2821 aacccgagtg cccgcttcaa tgaaacagac cgtttgatgg actactgcta tgacaattgg
     2881 tcagaaaaag agctcggaaa agctaacgca agtattccta gtctaaaaac cattgatgta
     2941 aaagacggaa aagaatcaac agttcctgtt gtgcttaaaa gccctgtaaa agtatgggta
     3001 cgaaacgata tggatactgg caaattaacg atcacgccaa cacttgacaa gaagcaagtc
     3061 actgacaatg agatccaagc acctatggat aaaggtacaa aaatcggtac cgctacgatc
     3121 accttggcag acgataaatt aggctattta gaagacaaca atgcaccatc aacagagatc
     3181 attacggata aaacagtgga aaaagcgaat atattcgtga ttggctggcg acgcgtcgca
     3241 gaatttttcg gcaacttgtt ttaattgaac aataagaaaa acatctttcc atcggaaaga
     3301 tgtttttttg ctttttctag tttaaataac agcttttttc atctattttc gttatactgt
     3361 ataagaaaaa ggagttgaat ttatgaatct aaccagagat ctgatctttt tagttctcta
     3421 tcttattggg gtattctcta tcgtttattg gcgaaaacag aagaaggatc cttccttgtt
     3481 cttttacggc agtacgtgga catttacggt aatcttaact tattttgtcg tgttggaaat
     3541 tttttattcg accttttatt ttttgattcc attgttgttc tttagtatct ttgctttttc
     3601 ttatttcaca gaaaaacgaa ggctactgaa tggtctttta ttcaatgttt tactcatcag
     3661 tttcggcatc tacttgtttg tcttgctgta tgaaacgcaa aatatttttc ttggcggact
     3721 gatcgccttg atcacgattc cattgctact tgtcctttta tttggtattt atggattgat
     3781 tgtttttctt ttttggaacg gggtcacagt tttacgtaga gaatcgcatt cattagctaa
     3841 tcttttaacg ttgatactag ccatttttct tacactgttt cttgtttttg actttttctt
     3901 gttaaaatat ttgccgcaat ggataaatgc tttgtttttc tgcgttcccc tgatcttgat
     3961 ctatctattt atcgtattct acaacttttt gactgtctca ttcttgtatc agttcaaccg
     4021 gcctcgatac aatcaagact tcatcgttgt gttaggcgct ggattgatca atggagaaac
     4081 agtttctcct cttctagcca agcgaatcaa taaggcaatt gctttttatc gggctcaaag
     4141 ccgtgctaca ttaaatccgc ccatcttatt gatgtctggc gggcaaggag cagacgaaaa
     4201 agtaccagag gccattgcga tgaaacaata tgccatggaa caaggaatcc ctgaaagaga
     4261 cattcttgtc gaaaccaatt cgacaaccac tttggaaaac atgctctatt caaaggaaat
     4321 catggatcag caaatgaaag ggccataccg cgcgatcttt agttccaaca attaccacat
     4381 ttttagagcc ggtctttatg cgcggcaagc caaattgaaa gcaaatggta ttggtgcaaa
     4441 aacagctttc tactacttgc ctaatgcgtt tttaagagaa tacctcgcca tcttgatgat
     4501 gaacaaaaaa agacatatga tcatttgcgg attgcttttc gcaggctccg cattcttatc
     4561 gctgattact ttacttttct aaaaaaacaa ggtgtgacaa aacatatgtc acaccttgtt
     4621 tttttctttc attctcttaa ctattggcaa aaagagtatc ccttttttga ccgctacttg
     4681 ctgagcagaa taaataccgc tgttgcctga acacataaag ctgatcaagc agatcataaa
     4741 gaagtatact gccgcttgtg aaccgaacaa ttcgatcccc atcacaaagc aggcaatcgg
     4801 tgtatttgtc gcaccagaaa atactccgat aaagcccaat cctgcaagaa aaggaacaga
     4861 aacatggagc aaaggcgcta gtgctgcacc aagcgttgca ccaatttcaa acaatggtgt
     4921 cacttctccc ccttgatatc ccgcgcctaa agacaaaact gtgaagaata acttcccaat
     4981 aaaatcatag acatgtgcct gtccattaaa tgattcttga agtaacggaa gactcaatcc
     5041 gagataacgt tgtgtttgca aaatcaatgc tgctgccaca actacagctc cacctatcgt
     5101 atttcgacaa acaggatgag gaatccattg tgcatatcgc gcttttaaaa agacgatcga
     5161 acggctgaat acccaaccaa tcaaaccaaa agcaatccca gcaacaaaaa ctttgaagaa
     5221 cagcaacata ctccattcag gaatcacacc catttggtaa tgtagatgag aaacaccata
     5281 gctttctgtc atgaaatttg caaacagtgc agcaaagaag ctagggaaga tcgcttctgt
     5341 tcggactttc ccgattgcca acacttctaa tccaaataat gttccagcta aaggtgtgcc
     5401 aaatacagaa ctaaaacccg cactgattcc actgataatc aagatttccc gttctgattt
     5461 atccaatcgg aacaaacgtg ccgtattatc tgctaacgct ccccccattt ggactgctgt
     5521 tccttcacgg ccaacagatc ccccaaataa atgggtagtt atggttccaa acagtgtaag
     5581 tggaataagc cgcaacggga ttttttcttc tccaccattt ccttgatcga tcactaagtt
     5641 gtttccacga ctagaaagtc cgccgtgata cgcatagaga taagcaaaaa gcgcaccact
     5701 aactggtagt agacaaagga gccaaggatg tgatagtcgg atatctgtca caactgtcag
     5761 gctatttaag aaaaaagcag acaagctccc catgaatata ccgagaatcg ctgtgatcac
     5821 cagccacttc agcatataca ctaaaatgcg tactggactc ggtaattgtt tttccataaa
     5881 aaattcctcc tcaataaaaa aacgcctact aaagaaaggg gctgtcccca gactttagta
     5941 ggcgtcatta gctatctttc gttagcattt aaaggcgaac accatcacct attcacttcc
     6001 attaaaagat agcatatttt tctgtaaaaa acaataaaat tatcgttctt tctttacacg
     6061 ttcagcaaat tcatagaaag gagccaattt cgttaactgt tgattacatt ggtcactatc
     6121 atgacaaata taattcccct ttttcgtata cgtgccatca gaacctgctt tcgttgtaga
     6181 aagaaaaagc gaaacattcg atgttttctg acagattgca cagattccct tatttatcgt
     6241 tggtgataaa gtaccgctaa tcccttttaa tttttcatct tcgtaataga ccatgaattt
     6301 tttctgcgta cctggatcat tccagccgat gtacgtatgt tcttgcaagt cgatttcatt
     6361 ccaagctggt tgtttgagtt ttttcacttt tcggaataat ttctcgattt gtggtttgct
     6421 aggctgcata aacggctgaa cgatttgctt cagctcctcc aatgctcttt ctgcttgcgc
     6481 ttttgtcagt tttacgtcta aaactgtcgt taaaaatgta tcgattctgg cagttcgtga
     6541 tctaatatgt ctcgaatctt ttcagctgct agtgcctgaa ctgtttgtac cgtttttgga
     6601 tcattgacag atgagtacgc atttaataac tgttctactt ctcttattat aaaaaaatat
     6661 tggtatggtt ttattgtttg ttccatgatt ttaaagtcct ttcttctaac caaagtgcat
     6721 actttgtaga aaaaagtgat cttctgcaaa gtatgtgtta cctgaagatc ctggactctt
     6781 ttaaataaga tacgttttgc atcttatcat cacccccttt aatgcttatt attctatgta
     6841 catgattcat tcgataaata aaagtaaacc gtatcttgaa cagatagtca actgcaaaca
     6901 gaaaaaagga ctgtgacaaa agtcgtgtca aagtcccttg tttcgaatga atggtgttca
     6961 aaagcttgca tctgcggtaa taagccgaaa cttcacaaaa atttgagaag taattttcgt
     7021 aaattccttc ttatttcttg aagctgacca cttctgtcac aacctcaata tggtctatta
     7081 ttggttttac ctgttaaatc gaggattctt tcccaaagta acgttgccat ctcttacgca
     7141 tacttgcaga catgggctga gccgcttctt tgactgcgca gtatttgtcc acgaatgtca
     7201 caatatatcc ttctttatac ttaggcggtg cgatcgttgc accccacatg tgcttcaaaa
     7261 tgatatctcg ctcaagatcc gacaaatcgg tgattttttc tgcattcttt acagcaattc
     7321 ttggatggat atacgcatgt gtcccctcgt caaatttcgt cgtacgccaa tcatagtaga
     7381 acagatcatg caaaagtcca gcacgcgctg ttgctttagc attgccaccc catttttttg
     7441 cgagcagata actatagtaa gaaacactga tggaatgctc caaacgagtg gaatgaacat
     7501 gctgtgtgta attcgctaat tttttcactg cttctgtttc taaaagatct tgtacataag
     7561 acatatattc ttcgtcttca cgccattttt ctgtcataat tatcgatttg ccaccttttt
     7621 gaatttcatt ttcttctgaa attgatctaa gtataccacc agtgttcgga ttattctagt
     7681 cgctctagat aaaatagcta aagcttaata gttccttaat cataaagaaa aatgataaag
     7741 aacgatccct gctgcgactc cgacattcaa tgattccgca tttcctttca ttgggatata
     7801 tacgttctga tcagtcgaag acaagatttc ctgacttact ccctgacctt cgtttcccat
     7861 gattatagcg aaatcctctg ttttttctac tttgtctaat gcatgtgctt cttcatttaa
     7921 ttctgtaccg tacaccgtaa accctttgcc tttcattaca gggatcactt ctgccaaatc
     7981 tttttgtaac acaggcaaat gataattgct tccctgcatg cttcgcagca cctttgtact
     8041 ataaatatct gccgttcctt tacctaataa aacaccagcc ataccaaacg catctgccgt
     8101 acggatcatc gttcccacat ttccaggatc ctgtacatta tctagtaaaa gccagcctcc
     8161 tgaaaaatct ggcgtatctt cagatgttaa cgtcatcact gctaagatcc cttgcggtgt
     8221 aggtagatct gacatagcgg tcatgacctc atctgaaact agaaaagtag aaatctcttt
     8281 gtttacagcc agccagtctc cccattcttt catacctctc tgtgtcacaa aaagttcttt
     8341 tatttctgca tcagacgacg cagcttcttc gatcagatgg aacccttcca aaagatattc
     8401 tgccttttct tctctgtatt tctttttatg tagttttttt aattctttga ttcttggatt
     8461 ttttactgat tggatctctt tcacattcct cacctcaact gattatatca tggaataggt
     8521 gcttgagctt gtttcatcct aaaaaaatcg gtatcattga tttataaaac aggaaagaaa
     8581 ggtgacgaac atgcgcaaag ttaaaatgaa tgtccaagga cgtgtccagg gcgtgagatt
     8641 tcgctatatg accaagatgg ttgctgatca gttaggcgtg accggaacgg taaaaaacga
     8701 agacgacggc tcagtcacaa tcgaagcaat cggaaatgac gacatcatcc aaaaatttat
     8761 cgaagaagtc aagaagtctc cgagcccaag tggacgagtg cagtatgtgg atgtccaaga
     8821 agatccgatg attgaggaac gaaagaaatt tgatgtggtg ggataaggtt gtttttgaag
     8881 cgctttgcct cgagtattaa ggagaaacat tttccaaata gcttctcata ttttgaaatg
     8941 taatgactta ataccgagag gcagctgata aaacaccgtc aataaggttg tgaaaaagct
     9001 ggtctgcatc aagcattaag gactggttgt gaaaaatagc tctccatatt tttcaacagg
     9061 ctggacttaa tgccgtagat gcaagctttt gaacatcgtc gataaggttg cgaaaaagct
     9121 gatctgcatc aagcattaag gactggttgt gaaaaagtcg cttagttttg agtattaagg
     9181 aaaattttgt gaaaatggct ttccacattt ttgcataaat ttgacttaat accaagaaac
     9241 tgacttttga acatcgtcga taaggttgtt tttgaggcac tttgcctcaa gtattaaaga
     9301 caggtagtaa aaaagctgat ctataacttg caataaattt gcttaaccca tattttccag
     9361 acagaacaaa aaagttttta gttttttaaa taagaaagaa acacttggaa tcactctttt
     9421 ctttctaagt atttctttct tgattttttg tcttttttca aaatttttcg ctacgaaaaa
     9481 gaacgaatat ttatatgaaa tttctatgaa gttgattgaa ttccatgcgc ttttttagta
     9541 tagttaagtt tgtgtaaaaa ttatagaaga ggaagtattc tatgaataaa tggaaaaact
     9601 ggttattggg atccggactt gtcagtatcc tgctcttttt atctggctgt gtgcggatgg
     9661 attcaaacgg aaaccctgac acttcaggaa tcgtttatcg agtactcgtc catcctatgg
     9721 gccaagcgat tacatatcta gttgagaatt tcaattggtc ttacggctgg gcagttattg
     9781 caatgactgt gatcgttcgt atcatcatct tgccactagg tatcagtcaa tcgaaaaaaa
     9841 caatgatcca atctgaaaaa atgcaagcgt taaaaccaca agttgaagca gcacaacaaa
     9901 agctaaaagc tgcaacaaca cgtgaagaac aaatggcagc acaagcagaa atgcagcaag
     9961 tatatcgcga aaatggattg agtatgaccg gcggaatcgg ctgtctgcct ctattgatcc
    10021 aaatgccgat cttctctgcc ttgtatttta cggctcgcta cacagaaggt atccgtgaat
    10081 catcttttta cggcatcgat ctaggctcac caagtatggt gcttgtggcc atcgcagggg
    10141 gcgcttatct gcttcaagga tatatttcaa caatcggtat tcctgaagaa cagaaaaaga
    10201 cgatgcgtac gatgctgatc gtctctcctg ctatgatcgt cttcatgtcg atcagtgcgc
    10261 cagccggtgt tacactttac tgggtagtcg gtggtgtgtt cagctgtctg caaacattca
    10321 tcacaaacgt tataatgaaa ccgcgtttga aagcacaagt ggcagaagag ttgaaacaaa
    10381 acccgccaaa acaagtcgtt actccaagaa aagacgtgac agaatcagca gcaaagccag
    10441 ctgacaacaa acaagcaaca aaacaaatca aacaaccaaa aaacagtaca aacaacaaag
    10501 gccgcaacgc aggaaggcag cagaagcgga aataaaaata tatgacaaaa aactgtagac
    10561 aaataaaaaa atttctttgt tctacagttt ttttgttaca attaaagaaa acgttcgcat
    10621 ttcgaggagg aatgtataat gatgtccaaa taatcaatgt atagtcgaat ggaagaagac
    10681 aaaatttcgc tagatgcact tgttcctaaa aatcatttag tccgtaaaat tgacaaatta
    10741 ttgtcatttg attttattta tctcattgtt gaatcaactt actcgaccat tggtcgccct
    10801 agcgttgatc ctgtcgtctt agtaaaatta gtattcattc aatatttatt tggaatttgt
    10861 tcgatgcgtc agaccattaa ataagttgaa acaaacctag cttatcgttg gtttcttggg
    10921 cttagtttaa ttgataaagt tcctcatttt tcaacttttg gtaaaaacta tgcacggcgt
    10981 ttcagagaaa ccgatttgtt tgaacagata tttatcatta ttctcgaata agcagttgat
    11041 gctagattta ttcatgaaga aattttgtat atggattcaa ctcatattaa agcgaatgcg
    11101 aacaaacgta tttttacaag agatatggca cataaagaag ccattttata ccaaaaacaa
    11161 ttagaagaag aaatcaatga ggcaagaatt gccgaaggca aacgtccttt tggttcaatc
    11221 attcgtcagg aggtggtcga aagaaaattt agcatcgcag atccagaaag tggttattat
    11281 gtaaaaacag aacgagaaaa acagtttgct tattctgctc ataccgttgg tgatgaaaat
    11341 ggttttgtat tagatgtcat gattacgcct ggaaatctac atgatagccg aatgctcgtg
    11401 ccacaaatcg aacgcgttaa atcacgttat ggtgtagctg ctaatgctgc gtacaaaaca
    11461 ccttggaacg tcaagtattt aatcgaacga aaactacgac ccattctccc ttatatcagt
    11521 ttcgcacaac tactcgagat ggctataaaa aatatgtatc taaatctttt atttgtgaaa
    11581 gttgtccatt attgaaaaat tgtacagaaa gccaaaaaca ccaaaaagaa atacatcgac
    11641 atatttggga agactactta gatgaagcag aacatttacg ccatacggtg tacaatcgtg
    11701 aaaaatataa aaaaagaaaa gaaacgattg aacgcatttt tgcagacgca aaggaaaaac
    11761 atggaatgcg ctacaccaag tatcgaggct tggaaaaaat caggtgcata caatgcttgt
    11821 ttttgtcgca atgaatctga aaaaattagc tacttggcag tgggagcata tgtcgttata
    11881 tcttttatta tacaaaaata gacaaaagta tcagtctaca ctcttagtaa agaggaaact
    11941 gatgcttttg tctacagtct tagagagtgg gacaatagtt ttttcttctt tacgtctttt
    12001 cctctccatg tattttccta attttcttaa tttaacgcca tgagtgccaa gccaatgtca
    12061 atcttggctc cttgtttcct tcataagtgg aaatccgttt ccgatagatc gaaccttctt
    12121 tttcctctaa aagttttttt acattctgct ttgaaataac gccatgtatt attcactaaa
    12181 atttgtcggt ttcgctcact ttttgctttc gtacattgac tacgaacagg tcagcaccga
    12241 ccactttcac attcgtaaat tttgaagaca cgtataaatc cacttttatc ttttcttcga
    12301 ctaaagcgtt gaccgcaaaa gcgacatctg tttcagaaat atattgttcc atatctaatg
    12361 gtaaaatttt ttggttgatg gtataatttt taaacataaa gcactcgctt cctttctgat
    12421 tgggtttggt cactttaatc ctacagggat gagtgctttt tgtgtacctt aaaatcagta
    12481 aatatccatc cgtgaaaacg ggtggctttt taacagccct agaagggctc accactcaca
    12541 gccctatctc tctttaacta tttaaatgga tctacatact cttgtttact tatactgtct
    12601 ctaattcgat catctgcttc ttgttctcaa atatatattt caattgtttt ttgatttaac
    12661 ccaaccgtac tcacgtagta tcctttggtc caaaagcttt tattcccata cttatatttc
    12721 aaatttgcat gtttttcatg gattaaggtt gcacttcgtc cttttaagta gcccataaaa
    12781 ctagaaatac taatctttgg cggtattctt actaacatat gaatgtgatc aggcattgca
    12841 tgagcttcaa tgatttcaac atctttcctc tcacacaatt ttcttaatat ctggccaata
    12901 cttactctta actttccata aatcactttt cgtcgatact tcggggtaaa gactatgtga
    12961 tacttacaat cccatctcgt atgtgctaaa cttttatcgc taaagctatt tcgtccacgc
    13021 gtagaaacgc gcgttttttt gtttcgtacg ctacgccaac gaaactgact aaagtcaata
    13081 aaaaaagtgg ggcaataatc agattttttt ctgactattg tcccactctc tttttattac
    13141 tcttcatctt ctactattcc actaattaat tagttgacag atacaatgct ttcacatcct
    13201 gcacgaacaa actcgggctc tgaaccaatc acttgcgagt tgataaattc aatgaccata
    13261 ggaagattaa tccgacataa agtttaatct gttgtccttc cattatcagt ttagaaatga
    13321 tattagtagg tgtaccgcct aaaagatcgc atagtaccac aaaatcattt aacgaatcta
    13381 tgacagtaag agcttttgtc tgtatgtttc agtgccatct tctggtaata aaggtaacgt
    13441 gtagatattc tcttgaggtc ccataatcat ttttgtactt tttttcaatt cctcacaaaa
    13501 ttttccgtga ctaatcaata tcaattttct actcattttt ggctctccct tatttcgaaa
    13561 tcacacacaa tgcagataaa gccacacaca agtaatacga taaaaatcgc tcgagttggg
    13621 tcatattttt ctttcctaac aaccaataaa cacctcgacg atcccagcga gtaagatttt
    13681 agataatatc atatcgatat agttttgcat attcaatgtc acatcgccaa tcgttggtac
    13741 cataaaaatt taatatcaat cattgttgca actaatgctc ctaccatgaa cactcttaat
    13801 aaagtcgcta cgtccgttaa tacagttaag cgagattaca ctagatgatc tgttcctgcc
    13861 aaggcaagag ccagaagcgg ttgcctgccc atttgccttt ggtgtgtcgg agctgtccga
    13921 taaagttcac cgcccgatcc gctctggctt tgtcgtagtg acattccgaa agcatgaatc
    13981 ggcttggtgt gtattccttt aatttgggat agccctttgg tctggtttct gccatcagct
    14041 accacctccc aacagagcct ccatcgcatc ttctgctccg acaccaccgc catcaccggc
    14101 aatgatacgg cttctggacg aaggcgtcag accgaactgc tcggaaaatc ggttcataat
    14161 tttcagatag gtctgggcaa taaacacctg tggtacctgc tgccaatagc cggatagggt
    14221 tttcacgatg ataccgtgct gggtaatgaa ttcctcggct tccttccagc gggcgtaggc
    14281 ttggcactaa ccggcaaagg cagccatatc cacatctgtc aagattccga tggcctccat
    14341 ctgctttgcc agacgcttcc attccttctt tgcctctggt tccagccact tcggacaggt
    14401 cggtgccttc ttttccggcg ttgactcttt tgcattgagt gggcgattgc ccggatttcc
    14461 ctccagcttc ttcatggctg tcggggttga ttttcttcct ctggttgcca cagtcgtcac
    14521 ctcccttcaa tgtatttctg ccagtagaca gagaacagag ccaagtcgaa acctgactct
    14581 cttctctatc tgctaacttg tatttcagtc cactcttctg atgaatcgca cccgcaggat
    14641 tttcaagatg ccgcaggcaa attccaagaa gtttctgacc gcatcataac catcgtagcc
    14701 gtgtagccac atccttccac ctcgccgccg tcctcgtcgc tgatataaat gattctgtat
    14761 tcgctcatct gttgttttct ccagtctttt gtttttttgt agtacatata tcactctaaa
    14821 agtgattatt atcaagtcgt ttgaaagcca taatgtacac aatcattcga ctggtttttg
    14881 gttcataatt gaccgccaaa gtgagcaaaa gaaagggacc tctgtctgtt tcagaagtcc
    14941 ctttcggtat ttggttctta gtggttcagg ctgtattctc aaacctcata ctcatcttcg
    15001 aggtctgcct ctgcaatgat ctggccgatg ctgtcgtaac gctggacctg catctggatg
    15061 gcatccttgt ctgctccctc gtccatcatc ttcagcagct tgtctgattc ctttttgtag
    15121 gcccagaaaa ggatctcctg aaggctggtc tcctgctttt tcttgcgaag ggcgaccttc
    15181 ttctcttcca ccgcctcgtt caacttggtg aactctgccc aagcaaggat accgtctcgc
    15241 ttgattgcga tctccacatc cttcagctcc gggatggtct ttgcaccctt gatctccttc
    15301 aaatatttct ttgccttttc gctcattgcc attagcgttt cctccttgtt tttgggattt
    15361 tcccctttgg ttgtgtgtat attgccatag tctctcgact atatcaagtc aatccacagg
    15421 catatactac acaatcattt ggagctgtat ttgtatacat tatgccgatt tatgagagaa
    15481 gaaaaggctc cagccgttcg actggaacct tgggggggtc ggtttcttag gcggcgtggc
    15541 ggtgtagacc ttgaggtagc tgatgttctt ccagacgtcc gtgcccttca gtctgaccat
    15601 gccgtccagc tctcggtcga gggtgaactt gcggaaaagc ttgatgcggt tgaggctgtt
    15661 tgctagctcc ttgccgttct tggcatccag aactgcgtcc tcgatctccg caatactaat
    15721 ttcctgtcta aaccaaaggt tgctcaaggc ttcgggaatg ccgtttgcgg tctgcagggg
    15781 gttgaaagtt tccaatacag caggggtcga aaccacctgt ccgagctgga attttgcgtt
    15841 ccctaaaatc atctgcataa tttcttccat gtgaatcatc catttctgcc gttgtggtat
    15901 tccctttggc agtacagtac tatatatcac tctgaatcgt acatttatca agaagcggat
    15961 ttttgtggaa tcaccaattc gatataatag taattgcatg aaaaatctaa gcactggatt
    16021 ggtaggtgat gattatacgt gacgatcaca tttacacctg tgattcctgc cactacagct
    16081 tccctgccga cgaacagcca gagcgttgtc ctgactgcga aaagacagca actcggctgg
    16141 ataccgaaat cgaaactgaa gattactatc gtgttcgtgc tgaaatcaaa gctgagatta
    16201 aagctttgaa tgcgggttaa cgacaggtac tgaccggcgg ctcaacaccg ccggcttttt
    16261 attaccacat cttgcggaat tcgctgggac gaacaccttc caccttgaaa aaagttctgg
    16321 aaaaatagtt tgcatctgtt atgccaattt ttgttgcaat ctcttccata cttagatttg
    16381 taaagcgtag caagtgcttg gcctgtgtga ttcgtttctg attcagataa ccgacaacag
    16441 ttgtaccgta agtttcacga aaaatcttag ctaagtactg tttattgata aaaaactctg
    16501 cactcaactg atccagcgta atcttctccg tgtaatggtc atccagatat tgttttatgt
    16561 tctgcaattc catttttttt gtagagaaat tcggcacttt gtcaggattc caagaatctt
    16621 ccatcaaatg catcagcagt tcactgagta gaacattgat tttcatatcc cggacatact
    16681 cctgtccctc tgccaccata tgaaggctcc tcagcaagtc agcatactta tctgtttttg
    16741 atgttttaaa gaccggttga ccacctcgtt caaggtattt tttgtagact tccgtcatac
    16801 caatgccatt aaaatggcac caccgcaaag tccacaaatc atcttctgat gactcatgga
    16861 catacggctt caaacagtca ataaacacca tatctcctac ttgaagcgta tatttctgcc
    16921 ccttatactc caatgtgcct gaaccaactt ctaccaggaa acaaaggtaa gatgacaatc
    16981 ctgtacgcct agatgtatgt gccttcagag cagtcagctg tccaatttcc tgcaaataca
    17041 aaagagagga tcttgcaaag aacgatgccg tgtacagcac acgatttgac ttcaccgatt
    17101 tcaaagcgtt agaaaacagt tcctccatta caagatttct cctttctttt cttactcgaa
    17161 catttccgcc agttcagcag ccataatagc ttccatagct tcggcggctt ccttgttctc
    17221 tgcgctgacg ctgatatagg tcttcagctt cggctcagta ccagaaggac gaaccacaac
    17281 agagcagtta tcttccagca taaacttcaa aacatcagac ttcggcagac cattcaggcc
    17341 cttactgtag tctaaagttt caataacctt cttaccacca attatatcga gacccttgtg
    17401 gaattcaacc ataatttcct gcatcttggt aaaacctgcc gcaccgtcaa actcgaagga
    17461 atgcagggtg ttcaaacaat agccatactt tgtataaatc tcgttcagct tatccagcag
    17521 gctgataccc tgagtcttgt agtaagagaa catttcacag attagataag cagcgttaac
    17581 accgtccttg tcacggacat agctaccgga cagatagccg taagattcct caaagccgaa
    17641 gatatagctg tcaaccttac cagccttttc caacatacca atctgctcac cgataaactt
    17701 aaagccggtc agcacatcga tggtacgaac accatagttg gatgcgatac gttcacccaa
    17761 atccatggtg acgatggtct tgaccatcac agggtcagca ggcatttttc catgcttaat
    17821 tctctgagaa cagatgtagt ccagaagtag cagaccagtc tcattaccag acaacagaac
    17881 atagtcacca tgtgcgctct taactgcgat accaacacgg tcgcagtcag ggtcagtggc
    17941 caacagcaga tccgcattgt acttcttggc atactccata cccagagaca tagcttcctt
    18001 aatttcaggg ttcggataag gacaagttgg gaaattaccg tccggcagac gctgttcttc
    18061 cacaacggtc acattgctgt atcccatctc tgccagggtg cgggtaacag gcaccagacc
    18121 ggtaccattc agaggagaat acacgatagc aacattcttg tccacttctt cgccaaacag
    18181 gacggactgc cccttcactt cttcgatgaa agcagtcaga acttccgact gaatggatgc
    18241 aatcttagaa gcatcgccat ccatctttac atcagcaaag atgtccagct tttcgatttc
    18301 tgccagaatt gcagtagcag cctcggtagt aatctgacag ccgtctgcac cgtatacctt
    18361 gtagccgtta tatttagaag ggttatgaga tgccgtaatc ataacaccgg catctgcctt
    18421 cagataacga gtcgcaaaag atactgtagg aacaggaagc agttctggcc acaagtgcac
    18481 ctgaatacca tttgcagcaa agatgtctgc ggcagtcttt gcaaacacat cagatttgat
    18541 acgggaatca tagccaacca ccacggaagc ggcttcatag ttcttattca gatagtctgc
    18601 cagaccttgg cttgccttac ctacggtgtg tacgttcatg cgattagtgc ctgcaccgat
    18661 ggtaccacgc agaccaccag taccgaaaga caaattttta tagaaagagt cctcaatgtc
    18721 cttctcagaa taggaacgca gttcttctat aatatctgca ttggcatgat tcagccaacg
    18781 ctcatattct actttataat tcatattatt atcctctcac atccagaaac tgaatcgacc
    18841 cgtgaatgct gatttcagta gaatcggccg gcacaaaaag gcagtccccc ttatagatgt
    18901 ccaacgattc attatcaaat cgaacggtac cacagccatt gacacacagc aacacacgga
    18961 agctgattga atccgcacga tagtgtaccg tctgtcttct ctcagtgttg acaatcatac
    19021 gagagacctc gaaatgcttg catctgcaaa gcaattccgc agctacacca gcacgatatt
    19081 ttagaacacg aagcggctgt ttcggttctg acaacccttt caagttcgct acttccagcg
    19141 ctttgtctac atgaagttct ctctttttac catttttgtc aacacggtca taatcgtaga
    19201 gacgataagt cagattggaa ttttcctgaa tttctgcaat tagtgcaccc gcaccaatcg
    19261 catgaatcgt tcccgcctca ataaagaaca catcattctt tttgaccgga accttctgaa
    19321 gataacgctc aacagttccc tgagcaatcg cttcacggat cacagcttct tctgttattt
    19381 tctgcagccc atagattaag ctggcatcca gtacatacca catctctgtc tttccaagct
    19441 gcccgttttc tttttcaatt gcatattcat ctgtgggatg tacctgaaca gacaaatcct
    19501 ttttcgcatc aatgaatttg atcaggatcg gcaattctcc tttggtctgc ggatgcgttc
    19561 ccagaaactc tgggtgttct tgcaacacct ccgtcaaggt catcccctga tactctgacc
    19621 agacacaaca aagctcggtc catccgggtg cgtagagcat tccaagtttc cgcaagagga
    19681 gacatctcaa ttccttttgc aaattcatca ttcaggcggt tcccccccac agataatcct
    19741 ttgcggaagg cttcaataaa aacggtttct gtataacgag ccctcctccg ttttttaata
    19801 ctctaactca aatttctgtt tatctttaac attgatatcc ttgccaagct gtacttcaat
    19861 cagcttcagc tcagtatctg ccataatggt atgccgacag ccagcactca tggtgatcac
    19921 atcgccagcc ttaacaacct gctcatatcc atccacgatg gtcttaccag taccggccag
    19981 aaccacccac acctcgtcac ggttcttgtg gctgtgataa ttcatggagt gactagcatt
    20041 cagagtaacc ttaatggtca tgctctctgc ttctacatcc ataaccttat aactacccca
    20101 agacttctct gcgaacatga tctgctgctc gaacttgtca acgaaaggct taatatagct
    20161 ggactgttcc ttatcggaaa ccagaatacc ttcccgagat gcacagatga ccacatcatg
    20221 cagacccata gccaacacag gaacatccag ttcgttcagg atgtgtacgc catcgcaatt
    20281 ctcattcata acacctttac cgataatgga ttcctccata ccttcggtca gagtattcca
    20341 agtccccaga tccttccaca taccagcaaa acgctgtacc tgaatcttgt cctcatgctc
    20401 aacaacggca tagtcgaagg aaatcttagt cagagtatcg tatttgttga acagatcctg
    20461 ataatctaca aagtcaatca gctgatgtgc acgctccatg acatacttca gcttgtagac
    20521 aaacacacca ccgttccaca gtgcaccctg tgcgatatat tcctttgcca cagcctcaga
    20581 aggtttttcc ttgaaggtat ttacgaaagc agtctgctct gcgctctcag gaatgatgta
    20641 gccgtacttc tcggaaggat aagtcggttc aatacccatg agcaccagat tagcttcgcc
    20701 tttgtcagcc tgatcggaca gagatttcag ggcctcaaaa tagtcatctt ctacgtaagg
    20761 gtcaaccggg caaacaacta cggactcttc tggatcaatg cccatcacat ctaccagata
    20821 agcggtagcc aatgcgattg ccgggaaggt atcgcggcga caaggctcta cagatacacc
    20881 cacatcctca ccaagttggt tatggatagc agaaacctgt gtcttagagg ttgcaatcgt
    20941 aacagttgcg tcgttatcta ccttcttaat ctggcggtag acacgctgaa ccatggattc
    21001 atatccatct tcagttttga aaatcttaat gaactgcttg gaacggatgt cattagacag
    21061 cggccaaagg cgcttaccag aaccgccaga cagtaaaaca atattcataa tgatctctcc
    21121 tttttataag aaagaggctg ctgcaatcct caaaaatcac agcaacctcg gtaaattagc
    21181 gttcagcacg catcgggtta ttcaggaagt cctcgtagga cagacggatg ccatcttcca
    21241 actcagtctt atatgtccat cccagcttgg ttgccttgga aacatccagc agcttacgag
    21301 gtgtaccgtt cggcttgctg gtatcccaca gaatctcgcc ttcgtaacca ataacctttg
    21361 ctaccatctc ggtcagttcc ttgatagaca gttccttgcc agtacctgca ttaacagtct
    21421 cgtcaccgct atagttattc atcaggaaca cacacaggtt tgccagatcg tcaacatata
    21481 ggaattcgcg cagaggagaa ccatcacccc agcaggtaac agtaggcaga cctgcttcct
    21541 tagcctcatg gaagcgacga atcaaagcgg ggagcacatg actgtgagta ggatgatagt
    21601 tgtcgttagg tccatacagg ttagtaggca taacagagat atagtcagta ccgtactgct
    21661 tgtttagaaa ctcacagtac ttcaggccag aaatctttgc cagtgcataa gcctcgttgg
    21721 tcttttccag ctcacttgtc agcagacagc tttccggcat cggctgcggt gccatacgcg
    21781 gatagataca ggaagaaccg aggaactgta acttcttaca gccattctcc catgcggagt
    21841 tgatgacgtt catttccaga atcatgtttt catacatgaa gtctgccaga gcgttctggt
    21901 ttgcgataat accgccaacc tttgcagcag cgagaaatac ataatccggc ttttcctgtg
    21961 cgaaaaatgc ttcaacagcg tcctgacggc acaggtcaag ctccttatgg gtgcgggtga
    22021 taatattgtt gtagtcctga cggttcagct cgcgcacgat tgcggaacca gccatgccac
    22081 gatgaccggc tacgtagatt tttgcgtctt tttccatcat aataatgctt gctcctttct
    22141 aaatttagag catctctcat tgcatgagag aaatcaattt gtctgcgatt tcgctcgcag
    22201 catcataaaa tgtatcaaaa tttgaggtgt ctagctgttt gaggtcctca atcacccgct
    22261 ctacgcggtg gaggttttcc ggtgtgacgg aaattgccag acccagtttt tgcaatttac
    22321 aaatagttgt tctatcttcc aaaactgtat cgcgggcgaa cagtgccatc ggacgctgtg
    22381 cgagaataca ctccgcaaca gttccccatc cagcctttgt aataacgtaa tctgccgccg
    22441 ctatatagtc ctgtgtattg ggggtgctgt tagaaataac ggttacgttt tctcccacaa
    22501 gcggaacacc tagcgttgta atgaaatcat acggcgcgtg actgacatca atcggctgtt
    22561 tgaaccgcgc ggacataccg agtgcgacaa atacaggcgg tttttcatgc tgtgatttaa
    22621 tgcgttgcac ttcgtcgtta tgaatcggac gcgccacaac ggaagtcggt gttttttcgc
    22681 tgttttggag aaattccaac atttcttcgt tatttagcgc ataaagcatg gtgtgctgaa
    22741 ttttaccgta acgcttggca tattctttcc aaatatgttc gggtaaaaat tcgcgataca
    22801 gctctgtcca agtgaagttt cctacataca ggaaaggtac tcctgccata tcgcaggcat
    22861 ccattgccca gattggcata tcgcaaagca ctgcgctgac agcttctcgc ttgagccatt
    22921 cggcttctgt ctttgccaaa gaaggaacct ttgccaaaaa atattcacag atctcggtca
    22981 gcttacccgt gtccaccagt agggtgccct cacggttaat cagaccgata tctgtgttta
    23041 ttggacgata gcgcacgcgg tctatcagct ctttgggcag ttcctgctcc aaattgcgct
    23101 ttgcaaaatc cagatgattt gcgccgcaaa ccagtgtaat cttcgcttcg catcggcaga
    23161 gtgttgccat aataatgggc agattccgcg cgatatgtcc gaagccgtga ctggaaagat
    23221 agaaacagat atgtttattc aatgtcagct tacttgactg cgttcttcat tgcagcttcg
    23281 cgctttgcca attcacggtc atgctttgcc atgatgttaa ccagttcctc gtaagaggtc
    23341 ttctgcggat tccaaccgag aacggtcttt gccttagtcg ggtcgcccca caggttgtca
    23401 acgtcagtcg gacggaacca ctgtggattt acacaaacga gcatcttgcc ggtttcaaca
    23461 tcgtagccct tttcatcaat gccagtgcct tcccaacgaa tggtcatgcc atttgctgcg
    23521 aatgccttct cggtgaagtc acgaacagtg tgctgctcgc cggtagcgat cacgaagtcc
    23581 tcgggagtct catgctggag aatcatccac atgcactcaa catagtcctt tgcatagccc
    23641 cagtcacgca gggagtccat gttgcccagt tccagatggt cctgcaggcc ttctgcaatg
    23701 cggccggcag cgagggtgat cttacgggtg acgaaattct caccgcgacg ttcggattca
    23761 tggttgaaca gaataccgtt gacagcgaac atgccataag cttcacgata ttccttagtg
    23821 atccagaagc catactgctt tgcaacagca taagggctgt aaggatgaaa cggagtggtt
    23881 tcacgctgag gaacttcttc aaccttgcca tacagctcag aagtagatgc ctgataaatc
    23941 ttagtcttct tcgtcaggcc cagaatacgg caagcttcca gaatacgcat aacgcccaaa
    24001 gcatcaacat cgccggagta ctcaggaaca tcaaaagaaa cctgaacatg gctctgtgca
    24061 gcgaggttat agatttcatc aggctgaaca atgctgatga tacgaatcag agaagatgag
    24121 tcggacaggt caccgtcatg aagggtgatc ttgcccatca aatggtcaat gcgagcggta
    24181 ttggaaatag aagcacggcg agtgataccg tgaacttcgt agcccttctc tagcaggaaa
    24241 tctgccagat aggaaccgtc ctgaccagta ataccagtaa tcagcgcttt tttcatgata
    24301 cattatcctt tcctatgtaa gtgataaggc catataaatg gctcgtaatt tctaatactt
    24361 ttcaagaata cacgataatt ttcgatattt catagaacaa tctacgcacg cattagcaca
    24421 atattagttt ttcgtg
//
LOCUS       DACKAC010000044        22464 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.044, whole genome shotgun sequence.
ACCESSION   DACKAC010000044 DACKAC010000000
VERSION     DACKAC010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.044"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            144..542
                     /locus_tag="I0B46_001774"
     CDS             144..542
                     /locus_tag="I0B46_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAR2084071.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            526..1221
                     /locus_tag="I0B46_001775"
     CDS             526..1221
                     /locus_tag="I0B46_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAR2084072.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(1815..2702)
                     /locus_tag="I0B46_001776"
     CDS             complement(1815..2702)
                     /locus_tag="I0B46_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAR2084073.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            2887..3726
                     /locus_tag="I0B46_001777"
     CDS             2887..3726
                     /locus_tag="I0B46_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAR2084074.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3922..4896
                     /gene="sgrA"
                     /locus_tag="I0B46_001778"
     CDS             3922..4896
                     /gene="sgrA"
                     /locus_tag="I0B46_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAR2084075.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            5194..5550
                     /locus_tag="I0B46_001779"
     CDS             5194..5550
                     /locus_tag="I0B46_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084076.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            5553..6710
                     /locus_tag="I0B46_001780"
     CDS             5553..6710
                     /locus_tag="I0B46_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084077.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6736..7176)
                     /locus_tag="I0B46_001781"
     CDS             complement(6736..7176)
                     /locus_tag="I0B46_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAR2084078.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            7336..7842
                     /locus_tag="I0B46_001782"
     CDS             7336..7842
                     /locus_tag="I0B46_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAR2084079.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            8010..9922
                     /locus_tag="I0B46_001783"
                     /pseudo
     CDS             8010..9922
                     /locus_tag="I0B46_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
     gene            9927..10928
                     /locus_tag="I0B46_001784"
     CDS             9927..10928
                     /locus_tag="I0B46_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAR2084080.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(11130..11336)
                     /locus_tag="I0B46_001785"
     CDS             complement(11130..11336)
                     /locus_tag="I0B46_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAR2084081.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(11982..12830)
                     /locus_tag="I0B46_001786"
     CDS             complement(11982..12830)
                     /locus_tag="I0B46_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAR2084082.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            12994..13896
                     /gene="gnd"
                     /locus_tag="I0B46_001787"
     CDS             12994..13896
                     /gene="gnd"
                     /locus_tag="I0B46_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAR2084083.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            13898..14899
                     /locus_tag="I0B46_001788"
     CDS             13898..14899
                     /locus_tag="I0B46_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAR2084084.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            14896..15180
                     /locus_tag="I0B46_001789"
     CDS             14896..15180
                     /locus_tag="I0B46_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAR2084085.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            15213..16010
                     /locus_tag="I0B46_001790"
     CDS             15213..16010
                     /locus_tag="I0B46_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAR2084086.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            16024..16857
                     /locus_tag="I0B46_001791"
     CDS             16024..16857
                     /locus_tag="I0B46_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAR2084087.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            16875..17732
                     /locus_tag="I0B46_001792"
     CDS             16875..17732
                     /locus_tag="I0B46_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAR2084088.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            17754..18263
                     /locus_tag="I0B46_001793"
     CDS             17754..18263
                     /locus_tag="I0B46_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAR2084089.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            18300..18701
                     /locus_tag="I0B46_001794"
     CDS             18300..18701
                     /locus_tag="I0B46_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084090.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            18813..19166
                     /locus_tag="I0B46_001795"
     CDS             18813..19166
                     /locus_tag="I0B46_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084091.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            19200..19643
                     /locus_tag="I0B46_001796"
     CDS             19200..19643
                     /locus_tag="I0B46_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAR2084092.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            19660..20745
                     /gene="uxuA"
                     /locus_tag="I0B46_001797"
     CDS             19660..20745
                     /gene="uxuA"
                     /locus_tag="I0B46_001797"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAR2084093.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            20738..21451
                     /locus_tag="I0B46_001798"
     CDS             20738..21451
                     /locus_tag="I0B46_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAR2084094.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            22203..22403
                     /locus_tag="I0B46_001799"
     CDS             22203..22403
                     /locus_tag="I0B46_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAR2084095.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         7745 a         3451 c         4643 g         6625 t
ORIGIN      
        1 tccatacatc cattgtactc tgagaaatca cacaatatcg aaacaaattc cgttaggaat
       61 gtgttattca ataaatttta taatctagaa acattttgaa taaaaactat tagacaaatg
      121 atattttacg aggaggaaca gagatgacga tctttgaaac ggttgtaaca gatgttggta
      181 tagaagcaga agtattcagg aaagaaaaaa taatgatttt atttggagat gaagcaccgg
      241 aaacattaac agattattgt tataagattg aattgaatca ggtaagaaaa gaaataactt
      301 gtgatatgaa gctctgtttt gataaacaaa tgtatgtaat cacagcagta ggagatgttg
      361 tccaaactaa tttgaatgaa ttaggacata tcacgataaa atttgatgga tctcatgagc
      421 cggaacttcc aggaacaatg tatgtagaag ataaggaaat gccgcattta agaaagggga
      481 caatcattaa aatcgatcaa gaggatcaga atgggggaaa gctatatgga aatcatgctt
      541 gatacagcaa atacaaaaag tatttattct tgtcaacagt ttttgaattt aacaggcgta
      601 acaacaaatc ctacaattct gaaaaaagaa ggagcgtcaa acttcttctc gcatatgaaa
      661 gaaatcaaaa aaataatcgg acagattcct atccatattc agacaatagg aagaacggaa
      721 gaagaaatga tagaggatgc tcgagttatc atagaagaac tgggaaaaga tacgtttatc
      781 aaggtgccgg tgaatcaagc aggccttcaa gcgatcaaaa agttgaaaaa agaaggttat
      841 ttgattaccg gtacagctat ttacacagaa ttacaagggt atttagctat taataatgga
      901 gcagattata ttgcacctta ttataacagg atgcaaaatc aaggcattga tgctatgaaa
      961 gtgattgatt cgttgcggac tgagatcgaa cgtacgcaaa gaccaacaaa aatattagca
     1021 gctagtttca aaaattgtga acaagtcact acagccattc gatctggagc tcatgcgata
     1081 acgttaggta aagagctact agatcaagta ttagagatgc cctcgattga taaagcagtg
     1141 aggggcttcc aagaagattg ggtgcaaact ttccaagcag aaacgattgg tgaactaaaa
     1201 aaggtcgctg attacttctg aatcaatttc tccataaaat aaacgatagc cttctcgaaa
     1261 actcgaggag gctatctgac gattcaatat tcattaattt aaagtttggg tgactattac
     1321 taactattac ttatttctca tttaaacgat gtattcccta acctctaata catccttgta
     1381 actctgaagt tacgccagtt ttgaataaat ttcataaaaa caaatgaaaa gtgtcgcata
     1441 aaaatatctt tcgtgtttct ctagtataac agaataagaa gaacaagtcc agaagggata
     1501 tttttagtct tttttaaaat aacttttcag tcgagtttga gtttatgata gttgtttttg
     1561 aagcgatacg gcaagtgaaa aaatagggaa gcatgaaaga atctacttaa tggcgcattg
     1621 attcatgtga tagaaataaa aaactttttt aaattatatc ctcatgattg taacaaatcc
     1681 agtgatttgt tttggtacaa ataaaaaaag gccccgatga agttgtggca aaagtggttt
     1741 gcctcaaaaa taagaaggaa tttccaaaaa tcacttctca aattttgcca catccccaat
     1801 tgggtcaaat ttgtttattt gaatatattt tttacataag gatctttaat agttacattg
     1861 tcttttttca tcactttacg gattgtttta tccatgaatt ccgtatcaag agtttttgct
     1921 tcggtagcaa ttttttctag ctcagatttg tatttgtctt tattagaacc tttatcctgt
     1981 ttttttacca tttttactag ataatagctt tgctgataag tcgttggatc tgttacagga
     2041 atgatatcag aaacttcccc gttttttagt ttaaatgctg ctttttttac ttcagtagga
     2101 atctctggat tggttgaatc aaaattcatt tttccatcag tttcctttaa tgttttattt
     2161 tttccataag cttgaacaag cttggagaaa ttttctcctt catttgcttc tttttttgct
     2221 ttatctgctt cttctttagt cgaaaataga atgatctgtg tactaacttc tggatagaat
     2281 tctttccaag cagtgtctag atctttatct gttaatttta cattggcttt caaaccgtat
     2341 tggaaagcca gcatctgttt aaagagtttc ttatatgtct cttctgtttg gccaactgct
     2401 tttaattgtt ccttgtagtt tggaccgagt tttttgattt gttcattata ttcttttgtt
     2461 acttcctttg aagaaatctc ttttccgtat acatcatcaa atgctttgaa gacaatcatt
     2521 ttttttaata gtgtttgatt atttgattta acttttgggt tatcataaaa atcctgaact
     2581 gtaagtgttc cacctttaaa cgttgcaatc gtttcattgg acttgctaca acctgagaat
     2641 agtaaaattg cacatgctga aaggcacata attagtagtt gttttttcaa aaaatttccc
     2701 atcctttcct tattaaaaac agtctaacag aagaattagg gagacagtct ttcattttcg
     2761 taaggttgct gtagatattc ttagttcgta aaaatctatt ctctctatga tggagaacat
     2821 ttacatagtt ttcacgagac aagaaagaaa agggttgtta aacttaatat aaaggaggga
     2881 tgaaagatga tcaggattat tcggttgatt ggatctgttt taatagtctt agttggtttt
     2941 gttttaggaa ttcttgtcaa taatgcactt gcggatatgt ttattggaaa tagtgaatgg
     3001 attggagcaa ttgccggtac ggtcggacat ttagctgttt ttttactagc tttatttctt
     3061 gctagtaagg tcgaaggaaa aagaatagaa gattatggtg tttctgttcg aggaaaagac
     3121 tgggggtatt tagcgggagg tctcgttgtt ggaattggag tgtttctgct tattactagt
     3181 ccattatatt tgacgggggc gtatcggtta gattcaggaa acgcaaacat tgtgccgctg
     3241 ataacgagtt ttattttatt tattgcagtg ggggcatcag aagagctttt attccgtggc
     3301 ttttttcagc atcagatgct tagatttggt ccgttaatag caatgattgg atctgctgca
     3361 ttatttgctc ttttgcacgg attgaatcct aacatgacat ttctggcagt tttcaatatc
     3421 tttttagcag gttgcttttt cagcgcactt atctacagta caggaagttt atttacggct
     3481 attggtgctc atattacttg gaattggaca caaggagcta ttttgggtat accagtcagc
     3541 ggaacgcaag aacaaggttt tttctccact ctgatccaat cgcaaaatac aataattact
     3601 gggggaaact ttggggctga agcatctata tcaactacat tattcttgtt agtcttgatt
     3661 gttggtttgt tactctatgc atataaaaca aagcggatga agaaatacac tgctgtttat
     3721 caataaaaga tgaagaaagc aaggctgata tcggtcttgc ttttttaagt ctgtcattga
     3781 ttaaacgaaa aagtaaacaa attcaagcga atacacgaca tcttttgtta gattttcaca
     3841 actgactgtc gtgttctcat cattttcatt ttatataatt cctttatcaa atagaaggta
     3901 attatatgga gagtgataga aatgaagaaa acagcgaccg tattatttgt tattcttggg
     3961 ctgagtataa ttgcagcaat gattggaatc agtacttctg caaatgaacg ggcaaatgag
     4021 aatctttcat ttactgtgaa aactgatcgg attgtttatg acatgacaca acaagtcatt
     4081 actattccag tcaaaccaaa caaaagcgtg aatgctagtg atgtacatgc tgttctaaca
     4141 tatggttggg atggtaatgg aagttcagaa aaagttattg gggaagttta tttaaaagac
     4201 gtacaatgga ccgctggtat tgagtataca ataatgattt ctgcagaact atcgattgat
     4261 gagatcaaga gtaaagataa agttgatttg attgtttttt atgatggaca gatgacgatc
     4321 acggagaatt tgaaaccaag cagttggaca gttgttggac cgccaagcag tacggatagc
     4381 acaagtagtg aaagcagtac agaaaacacg agcggtgaaa gcagtacaga gagtacgagc
     4441 agtgaaagta gcacagaaaa cacgagcagc gaaagcagta cggagagtac gagtagtgaa
     4501 agtagcacag gaagcacgag cagcgaaagc agtacggaga gtacgagtag tgaaagtagc
     4561 acaggaagca tgagtagtga gagtagcaca ggaagcacga gcagcgaaag cagcacagga
     4621 aaagtaaata atgaacttga tttggtacct atcataccaa ctaaggaaca aaagagaaat
     4681 gcagggagca acctaaaaag tgtgctaaaa cagcctgatt ttgtttctcg tggaattgat
     4741 catcaagaaa aagcgaaaga aacagataat agaaaaagcg atttaccaga aactggtagt
     4801 agaaccttga acaagctaat tacatggatg ggtgtgttac tcattttgat tgtaggagca
     4861 agctattttc gttctctctt ccatcgagtg aaataaagaa taaaagcaac agatctaaaa
     4921 ttgaataaaa cgatagattt ttgagtgaat atttcattcg agagtgctgt attgcctggc
     4981 gaagttgtag ctttgccaga tctatttttg tttactaaca tatttgagaa acaggaaata
     5041 acaggaacac aaatggaaaa taagagcaag aataattgac aatgcacact gcgcatggta
     5101 gtatctctga aaaatcagta acgaatctat ttgtacgtta ttcatgtagc tgtcggaata
     5161 agtctaaata atagataaaa aggagtatgt actttgtttg aagaatttat tgatatcaat
     5221 gaaaggcaag tatatcaatt tttgaactat tgttatgaaa gagacgagaa attatatgtg
     5281 gtgaaggata tcgctttaga tctaaactac accctggcta agatgaattc ggtgattcaa
     5341 caagcagaat cgttttgcga aaggtatcca gaatataaat tatcttttct ttcagaaaac
     5401 aagatgataa aagtcgagtt ttcttctcag tttttactga gtaaagttta ttctatactt
     5461 ttagagggaa cgattggtta tattttactg gattcacttt acaaaggtac ttatcaatcc
     5521 ttagaaaatc ttagtcaaaa aattatataa gtttgcgtac gattcaaaga aaattaagag
     5581 aacttaatac tattttgaaa aactatcatt tatcttgcag cttgaaaaaa ggtaatccga
     5641 tagaaggaga agaataccgt atccgatact tttttcactt gatgtattgg cagtcattcg
     5701 acgaaaatca ttctgatata ttatcaagta taaaaaataa acgtcaacta ttagaaaaat
     5761 ttgcgaaaca tgcgccatat acgcgtagta tagatagaaa gaaatggatc agcctactaa
     5821 gaatttcgtt gcaacgaatg aaacaaggcc actcgattac gtatgtccca gaagagatga
     5881 acaaatttgt gcatccattg ataacaaaag aagagtttac gcattacata ctagagcctt
     5941 tttttgaagc aaacttctgg tcgatgaatc acatcactcc aacagagcaa gcttaccttt
     6001 attttatgtt tggtgtaatg aacacctatt tggaagaaga tttgttggat actccagata
     6061 aagtacaaga aagttggggc tggattgaat cgtctattgt agaaaatttt gaaaaaaact
     6121 tcaagatttg ttttacaact tcagaaaaaa attatctata tgtcaatttg gcaatgatcc
     6181 atttgtattc aagggttttt gggacaaaaa agaaggtgga tgcttttgga aaatcagcag
     6241 aagacagaga ttttcagcat atatttccag tgatgtatcc aatcatgcaa cagtttttta
     6301 gtggggtagc tcgcagaatt ccagagctta atacttatta tgaaaagaac agacggttga
     6361 tttttcaata ctgtatgtta gtcagggata tttttataaa acacgaacaa cctgtcatat
     6421 tctatattca atcaaagtat ggtaaaacac aggaattatg ggcaaagaaa aggatattat
     6481 ctatgactga acgcccaata aaatatagtg catccgctga tcagttacct gatattgtca
     6541 ttagcgatta tcctatcgac aaaaaggcat atgatcaaac gaatacacag atattttact
     6601 ggaatggaca acctacaaaa aatgattggg cgcgtctgaa agctgaaatc ataaaaatca
     6661 ggaatgagaa aaatagatcc atattggaaa aattggattt cgttaattaa gaaaagcaga
     6721 ttgtttatgc caaaattaag acattgcaaa aagattattt tctttaaaat cgacaagatg
     6781 accagaaagc aagagttcgt tgagctcttg ctttctttta gttttagaaa ggaacatcct
     6841 gattttatct tcgctaaatc cagtcaaaag ttttgcagat tggtcgtaga taatcggttt
     6901 tttcaataaa gaaggggatg tacgaatctc aatatatagt tcactcaaac tgagttcatc
     6961 tattctgtga acaagatttt tataactaat agaatgagta gagagcattt cggtcacgcc
     7021 gttgtttgtc aattttagta agtggataaa ttcatttttg gttagtggtt gacgaattaa
     7081 gttctgttcg ataaacggta tatcatggac gataagccaa cgttttgcgc gtcttgaaga
     7141 cgagcagcca gaacctgtat acaatttaat catcatacat tcctccaata gctaagtatt
     7201 tatgaaataa taacatgaag aaatagtcga aaagtgccat ttagaggtgt aaaataagaa
     7261 aatgttgtca cgtataatgg aactagctag ataagaaata ctattaaatc aagaaaaaag
     7321 aaagaaggca caagtatgtc aaacacagcc gacgcaatag tagtgatcga tttacaaaaa
     7381 ggggtatgtt ttgattcttc gccgatacac gaattagaaa cagtgacagc attagtgaat
     7441 aaaagaatca aaacatacga aacgattggg aaaccagtta ttttcgttca acacgaagat
     7501 gctcaattag tgccacaaac aaaaccatgg gagctattgt cggaattatc tattcctgag
     7561 cacagttatt ttgtgagaaa aacccatgcg aattcatttt ataagactaa tttgagtgtt
     7621 ttattaactg aattggactg tcattcgatc gaattatgtg gggcacaagc agagtattgt
     7681 atagatacga ctgtgaagtt tgctcatgga ttaggatacc agcttatgat gtgtagagga
     7741 gcaacgacaa cttatgataa tccttttatg aaagcaaagg aaacaatcgc tttttacgag
     7801 aaaatttggg ataaacgatt tctgacattt ttggagcaat aagttgtttc ttcgtttaaa
     7861 aaaagcagaa tggatactgg tttatccatt ctgctttatt acgtaaaatg agaaaataat
     7921 cctgttctat ttttatggga taaaccattt attttttgta taaaaaatgt ttgcgtttac
     7981 atataattct cctagaaaca taaaaaggag tgaatagctt gagaacaaaa caagtattaa
     8041 agggagcgtt ggtcatctta tttgctgtaa gtacaagcgt cccgcaatgg acagtatatg
     8101 gagagagcat ttcttccgaa tcggaatctc gggaggtaga aaccacagaa actactgatg
     8161 ctggaaatga aacgaaatcg agtacttcac aaacagaaac aaatactgaa acaacggttg
     8221 agacatctgt tactagcaca gatcaagaaa cgaccgctac cacagataac caacaaacag
     8281 aagaagcaac agctacaaaa atgtcaaacg acgaatcctc ttctgctaaa gtaagtgaca
     8341 aagagaatgg gacagaaaat gatgatatcg gtaaaggtga aaatttcaat ggtaaagaat
     8401 atttgaatta cttcggtaaa cagatgacag aaatacttaa tggaccaaca gaagacatca
     8461 agtcaaaatt aatagagtta ttagtcactg agcttgctgt taatgattat ggtgtagata
     8521 aagagattat caaacatctg attgctactt atctattgta cgatatcgct tttgcaaaaa
     8581 acagtgtgtt gatgtcgatg cttggaggta cagatgatgg gacaacgcct acagaaaaca
     8641 caggcttcta tccaaataca aaacaacttc atcaaactgt actaaacaaa caagatggca
     8701 aaatgatgga tgtatatgca tattatgtcg atcaaggttc agataaaaca gtcatgatcc
     8761 atggcggatt tcgagggaat tggaataacg ggatcgttac agaagaatac aatgattttt
     8821 ataaggccgg gtataattta ttatttgttg attccagagc gactggtaat agcggaggcg
     8881 attatgtgac ctatggacaa tatgagtcag atgacgtcct ttactggata aatcaagaag
     8941 ttcgagagcg gccatctcaa aagattttac tttatggtgg ttcgatggga gcagctacga
     9001 tgatgtctgt attagcgaaa gatattcctg tcaatgtcaa aggaattatt gaaaattgcg
     9061 gatttgcatc aattgatgaa caactacgtt ttacgtacag ccagacagta gcccccgcac
     9121 taccggatgc cataaaaaat cagttagaca tcattgggga tcaagagcat gaagatttat
     9181 ttatgggcct tttgaaacaa tactattttg atcaggagat gcatttggat acaaaagctg
     9241 ctctacctac gattggaatg tcagattcat tgcctaaatt gattatccat ggaacagcag
     9301 atgatgtagt acctgtgtct aacgcgcaaa aattatatga gttatcagga gggtataaag
     9361 atcttttatt agtagaagga gcagggcatg gaaaggcaca ggaagttgac catgctgcct
     9421 acacgaaaca tgtaacggac tttttgaaag tagtgttcca tgatcagata aacgtaaagt
     9481 atgtggatga aaacaataaa agtctactca aagaccagga cgaaatccag ctttacggag
     9541 cttatggaga aaattatatg acagagcaaa agacattcga aggatacgaa ttagcaaatg
     9601 ttgaaggacc gactgaaggg atttttaatg agacgatacc aactattatt ttctattaca
     9661 aaaaaatccc agtagctcca ccaaaaaagc aagatccgac agagaatgca gacaataagg
     9721 gaaagaatga acctcgagag gatcaatcaa gtgatgatac gcaaccgatt cagatgacag
     9781 tgaaagtaca aaaaaagaca gatgcaaaaa aagaatgttg ccgaaaactg gagaaaagaa
     9841 acattcgctt ctcatttatt caggtggatt tttatctgtt agcacgatta tcacatggat
     9901 ttggcgaaga aagagagggt aaatgaatga aatggttcaa gcgaatagtg attgcagtac
     9961 cagttatcat cgtagttatt ttagtcactg tatttttgat taatcaaatc acaccaaagc
    10021 cgattagctt gcttgttaga aaacaatttg acacaagtga aaaagaacaa gtatatgccc
    10081 gaccagatga tttccagcaa aaaatcgaac aaatcgatat aaaaaaagaa atatcctatc
    10141 cttcaaagta caaaaataat aatatggata tctacacacc gaaagtggaa aagaaaaaat
    10201 taccgattct tttctggatg catggaggcg catatgtagg aggggataaa aatgattgtc
    10261 gtgattattt ggaatatcta tgttcagaca cgcagcaaat catcgtaaat atagattatg
    10321 aacgatcacc ggaagcaaaa catcctacac cagttatcca attgaacgaa gccattcaag
    10381 cagctaaaaa agagataaaa gatcaagcag attggtcgaa tatttcgatt ggtggaaatt
    10441 cggcaggtgc acaaattagt ggagaatatt tgttagcact tcaaaatgaa gaaattaaaa
    10501 aagctgatca tcttgatccg actttggaga acaagcagat tacgaagttt atttctttgt
    10561 ctggtctgtt ggatccttct gcacttactc aagtaagtga taaaatctct agctttcttt
    10621 atgaaaaatg tggatgggct tactttgata ataaggactt tgaacaatca gagaaggtca
    10681 aaagtttagc cttggtcaga catgcggatc gttggacgca aaacacattc ttaacggatg
    10741 gaaatacaaa tacgtttacg aaacaaatgg aagaagtaac ttctgtattt gagaaagcgg
    10801 gggtgaaagt cacaaaagta gattatgcgg agaaaaatgc tgttttgaac catgaatatc
    10861 agtttgattt cagcaataaa caagcgcaag aaacatatca aaagttagta atgtttttca
    10921 aggagtaaag tcttatgatg caaaagaggt tgtaatctgt cacttagctc caagtcacaa
    10981 ggagcaattt caataaacaa gcttttcatg gtttatgaat attgtaactt gtgactgagg
    11041 agttagagat ggcaaggggc accttttatt cgaaatttgg gaatatcaca cgattattgt
    11101 cacgatctct tttacgtaaa ttttataagt cagatttgtt gttttgatcg tttaaaaaaa
    11161 gcaacaaatc cgaagcctgc ggaggcaaga agggacaaaa agccgagtgg taagcaaaag
    11221 aatgctggtt ccactaatat tccgttgtta ttgatataag gctgggaaca aagatgaata
    11281 gcaaataaaa tcaatgcact agcgaaaaaa agcaaagaaa tatggatagc tttcataagc
    11341 agcacctcct tttttataat tgtatgataa atagattaag aatctataaa tgtgacaagt
    11401 ttgttaggat gtgaataaaa tgatcatcta gcgaacttga aacaaagatg gctaatgata
    11461 agcaattaat gattcagcaa tcagataggt acaaagttta ccatcatacg tctagaccaa
    11521 taatagaaaa tatgggaata taatagaaaa gatttaggat acccccaaaa gaatttcccc
    11581 aaaaattcga tgtatcctaa acttttttat tttacataac aatcatatca actgtgaaat
    11641 aaaattacaa atgataacct aaaatgaggc caaaagccag tgttatcaag gaaatactta
    11701 attcaccaaa atttttttta attttttaga aaataagaaa caaaaataat aaaaaaaaga
    11761 aaagataaat tttttactac tgtatactaa ctttgttatc gcatctaaca agtaactcga
    11821 ataataccaa tacaaccttc tccaaagatg cgatgacata cgttctaacc caccgactat
    11881 tgatgttctg taccaccctt gcagaaccaa aaaagccact accaaatatt tggtagtggc
    11941 tttttttgtt ttatggattt ttcgcttttt ctgttatggt gctattcttt gatttcagaa
    12001 atagtatgtc gaactttagc gatagaacga cgtgattctt tttcgttgta aaacatcaaa
    12061 atcacatata gagagtccaa aatgcttagt tgagaaatac gggaagctaa agcttcagat
    12121 cggaattctg tttcttcaga aatagaaata aaaacgacat cgccaagttt agcaagggaa
    12181 gaattagctt ggctagtaat aacgattgtt tttgctccag tttttcttac ggtttcagca
    12241 atatggattg tttcttttga ttgccctgtg tgagaaatca aaacagcaca gtctttttcc
    12301 gtcaataaag aagcttccat caattgaata tggtaatccg tgctgtgaga agttgcgata
    12361 ggggaacgca aaaatttatg ataagcgtca gttgctacaa tctctgaacc gccaacaccg
    12421 aaaaaataga cacgtttaga ttcattgata atagtagccg ctttttttag atccttttgt
    12481 tgtaacaact tttttgtgtc atttaaagtt gaaatactag actcgaacac cttttgcgcc
    12541 atagtgagct cagtatctgt tttctgaata ttttcgtgga tagaaatagc agatagatca
    12601 ttttcttgga tcaacatggc cattttaaaa tctttaaatc cattataacc aagttttttt
    12661 gtaaattgaa aaaaagtaga gtcagcaatg cccagttcac ttgctaaatt gctaatagga
    12721 ctatgactta ctttgcttgg attttctaaa atataatcgg cgatagcctg ttcttttggg
    12781 ctgaagtcgt tgtacattgt tttgatttgt agtcttatgg agacgctcat gagaattcct
    12841 cattttcata ataagattat cattttttcc gttctcatta atgttaaaga tacaaggaga
    12901 gtaggaaaaa atcaaacaaa aaatattttt gtaaaatcta tttacaaaaa aatttttttg
    12961 tataatgagg gtataagaaa tggaggcgct atcatggaaa ttggtgtaat tggtttggga
    13021 aaaatgggat tgaatttagc tttaaactta aaagagcacc atcacaaagt acaaggactc
    13081 gacatttcaa gtacagctgt tagtcaggca agagaaaatg acattcctgc ttatcaagag
    13141 atctctgaat ttctagctac ttttgagcag aaaaaaatca tctggttaat gcttcctgct
    13201 ggagagataa ccgaaaacat gttaagaaaa ctttttccaa aactcggacc aggagacatt
    13261 attattgaag gcggaaactc ttattataaa gattcaatcc gtcgagcaga agagtttcag
    13321 aaaaatgaaa tcggttactt ggattgtggg acttctggag gaatcgaagg agcacgacat
    13381 caagcctgct taatgattgg aggagataaa gaaacttttc tatccgtgga acaattattc
    13441 aaagatgtag caattgaaag aggatatctg tatgctggtg catccggaag cggacacttc
    13501 ttgaaaatga tccataatgg aatcgaatat ggaatgatgc aagcaatcgg tgaaggattc
    13561 caacttgtac aaaaaagtga gtatgctttt gacttggaac aagtcgcacg tgtttggaat
    13621 catggttcgg tgattcgcgg ctggctgatg gaaatcatgg aagatcagtt aaacgaacat
    13681 cccaatttaa gtaattatcg gggaatcgtt tctgcatcag gtgaagcaaa atggacggtt
    13741 gaaacagcgc tagaaatggc tgttccagtt ccagtaattg caactagttt gttcatgagg
    13801 aatctttcac aggaagaaga tagtttttca gcaaaagtag tttcagcact aagaaatggg
    13861 tttggcggac atgaaatagt tgaaaagggt gagtaaaatg ccagattatc atatagcaat
    13921 cgttaattcc agcagtttcg gaagagtctt tccacaccac ttggaaagat taaagtcagt
    13981 tggagacgtt catttcttta ctgttgatca agagattcca ggaaaagaac tagcggagtt
    14041 acttaaagga ttcaatatca ttattgcaag tgttacgcca ttttttcgac gagatttttt
    14101 tgaaaacaaa gatgaactgc tattgatttc ccgacatggt attggttaca acaatattga
    14161 tttaaaagca gcaaaagaac accagacaat cgtatctatc atacctgctt tagttgaacg
    14221 aaatgctgta gcagaaaata atataactaa tttgttgtcc atgcttcgac gcacgacaca
    14281 agcacaagca agagtcaagg acgatcgatg ggaagatcgg gcggagttcg tcggtaggac
    14341 gctgtttaac aaaacagttg gtgtgattgg tgcaggaaat accggaagcg gagtaatcga
    14401 aattttgcgt aacggttttc gctgtgatgt tctagcttat gatccttaca aatcagattt
    14461 atacatcgaa agctttggtg caaaaaaagt agaacttcaa gaattactgg aacaatcaga
    14521 tatcatttgt ttatgtgcga atttgacaga agagaactat cacatgattt ctgataaaga
    14581 agtcgatcag atgaaagaaa atgtttatct atctaatagt gcaagaggcg ctctagttga
    14641 tgaagaagcg atgattaatg gtctgaaaag cggaaagatc gcaggatatg ccacagatgt
    14701 tttagaagaa gaaccgggac gtcaagatca tccgtattta gcgtttgaac aagtagtgat
    14761 tacgccacat atatcggcct acacaaggga atgtttagaa gcaatgggtg aaaaatgtgt
    14821 cagagatgtc gaacagatca gtcagcacct tcttcctgaa cgttctgtcc agacaaagag
    14881 caactatata aaaggatgat gaagaaaatg gtgaacggtt taaaagtgaa aacaggccct
    14941 caattttatt tatatgaaga aggtggtatt tcaaaagttt ctgatctctt gaaatcatac
    15001 ggcgcgaaaa gagtactggt cacccacgga acggtatcat gggaaaaggc gctacctaaa
    15061 ttagtatttt tgaatgacga aacgattcaa tttttttatc atcgttattc tggagaatgt
    15121 agttatgctg aagccagacg aatagctaca atcatcaaaa aaatgaaatc gatttcgtag
    15181 ttggcgttgg cggaggaaaa ctgaccgatc tagtgggtta tagtgccaat cttgcaagaa
    15241 tcgatttcgg agtcatccct actttagcta gtaattgtgc tccatggaca cctcttgctg
    15301 tcatgtataa ggagaatgga caagcagaag ggaaaacaga acacttttac agacaagctg
    15361 ctttttttat aacagatccc caattagtga ttgattcccc tgttccatat ttcattgccg
    15421 ggttagcaga tacactagca aaatggtatg aatctgagct catcttagaa caagattttt
    15481 tgcaagatga atcatttttg aagctagcac aagatactgc gaagatctgc aaagaagaga
    15541 ttttgaatca ttctgctaaa gcaatccaag atatggcaca aagaaaactg acatcggagt
    15601 tcaaacagct ttcagagatt atctttgctg ttgctggttt agttggagga ttaggagata
    15661 aatacgcaag aaatgcggca gcacatgcta tgcatgacgc tatcagtaaa tatattccgg
    15721 aaagccatcg ttttctacat ggagaaaaag tagcgtatgg gattttctat caactggcac
    15781 tagaaggtcg ctgggaaaaa atagacgagt tatatccttt gtatcgggaa ttagaactgc
    15841 cggtttctct agaacaaatg ggattatttc cgaaagataa acagataata gataaaatgg
    15901 ctgaatttat tgattcgaaa gagaaagtgc atttgattcc tatagaggta acacagaaat
    15961 cactgataca gtcaatgatg gaactggaag gttatatcag aaagatctga aggaggagga
    16021 cacatggaaa cattaagtgt agtacaaagt ttgttgattg ctttatgggt agccgcggtt
    16081 atgtcccgct ggttaggtgg aggagccacg ttgaccttac gtttttctcc tttgatgact
    16141 ggattagtcg tcgggttgat catgggggaa gttgcacaag cgatgattgt tacagccgct
    16201 ttgcaaatga tttatatggg ggttttttct ccaggaggat caatgccagc tgaaccatct
    16261 attgcagcag ctatcgcagt accagtcgca ttattagggg atctgtcgcc agaagcagcg
    16321 attgccgttg cggtgccagt cggattgctc ggaagttact tgtatcagtt tcgttttttc
    16381 atcaatactt ttttaggtaa atatacagac aaagctgtag aagatttgaa tacaaaagct
    16441 atggcccgat caattattct ttatccaacg gttgcctcat ttttattatt cgttccgctt
    16501 gttttcattg ctttatattt aggggcgcca ttgatcgctg atgtaatcaa agctttagag
    16561 ggaacagtcg ttatccacat tcttgaagta gtaggtggag ggttagcggc aatcggtatc
    16621 gcgacaactg tatacgttat cggtcgaaaa gattatctgg tctttttctt ccttgcttat
    16681 tttatgagtg ttgttttgaa atctttagat attacgatgg ttacttatgc catttttggg
    16741 attattatcg ctttgatctt tgtccagtcg caaaaaggga aacaagtaca gcctgccaca
    16801 agcaataaca attcagcatc ttttgatgac gaagatgact atgatgacgg attttaattt
    16861 aggaggaatg ggaaatggct gaaataatgg ataatcaaac aggtgctcct gaagaaatca
    16921 cgaaaaaaga tgtaacaaaa gcctatctga gatggcattt tgcaaacgaa attcctcact
    16981 cttttgaacg ttacttggcg ccatcactac tatacggaat gatgccaatc ttgaaaaaac
    17041 tttataaaga tgaagactct ttaaaagcag catataaacg gcagttactg ttcttcaaca
    17101 ctcaattaag ctggggtggc ggtgtgatta ctggcctaat gtcttctatg gaacagcaaa
    17161 gagcagaaga agagtatgaa gaaaaagaga tcttgatgca ggatgacttg atgtataaca
    17221 cgaaagcggg attgatgggg gcactagcag gaatcggaga tgcaatcgat tcaggaaccg
    17281 tccaatatat ttttatcgca atcgcggtac cttgggcaca acagggaagt cctttaggcg
    17341 cactttttcc ttttatctgc tttgggttgt atcaggtttt actaggggta tttttcgcaa
    17401 gacaagcttt cactatgggc cgtaatgcta caggcctaat gcaaaacact ggtgttcaga
    17461 cagcgattga aatgctatca gttttagggt tgtttatgat gggggtgctt gcgggaaact
    17521 atgtaaaagt agaatcttca ttgaaatttg cgatatccgg acgtgagttt gttatccagg
    17581 aaattcttga tcaaatcgtg ccagggatat tacctttagc tgtcgtaatg ggtgtgtact
    17641 tgttctatac gaaaaaagga ttgaaagtca cacaagcatt attatggctg actggaatat
    17701 taattgtgct agcaggaatc ggtattttat aaaaagaagg gaacgtgaat aagatgggtg
    17761 tagtcaattt agcgcgtgtg gatgagcgtt tgatccatgg gcaagtaatg gtaacgttat
    17821 ctcaaaaaag tggagtgaat tccatttttg ttgtagacga agttgtagca aaagataaat
    17881 ttatgagaga tctttacaaa aatgcaggaa gccgtacagg acaaaaaaca attgtcatta
    17941 caccagagaa agctaaattc tattgggatg aatatcaatt taaagaatac aattgtattt
    18001 tgatcgcaaa aacagtccaa acgatttacg acttagtaaa acacggaatc cccatggaag
    18061 aattgaatat tggagggatt gctcaaaaaa atcctgatac agatctttta gtgacgaaat
    18121 ctgtttattt aaataaatca gatgctgaaa aattgaaaga aatgaatgaa caatatggcg
    18181 taaagaatat ttattttcaa gccactccat ctgcacctaa aacaggatta aaagatgtat
    18241 tgaacaagtt tgatttgtca taaacaaatg cttagaaaaa agataaagag ggcgaaaaaa
    18301 tgaagaagaa cgagctgttt cgtgattggg aatttcgtta tcgctatatt taccgtaagc
    18361 gccgtacaaa aaagagtaaa cagcgttttc tttcagcatt agtatcagat atttacagta
    18421 tgagaacaga tgtgacagtc attgcttacg atacgctagc ctaccgatca aaaaatattt
    18481 atgttggaga tatcgaaaaa gcagagaaag ttatctgtac ttattacgat acacccgttc
    18541 atgcattagg aagttatttc atgtttgatt ggaaagacca aaggaaaaaa acaatctact
    18601 ctatattatt gtccttcatt ttgttgttca gtttaggttg gtggggaatg atgatatata
    18661 acaaaaatcc tcatcacgta ttcgatctgc tatccgtata aacaggtatc actgtactag
    18721 catttgggag ctactttttc ttattgggaa aagcagctcg cggatggcct agcagacaaa
    18781 cttttattcg aaatacttca tccatattaa cgatgctaga aatgatccgt acgattgatg
    18841 atccgagtgt agcgtatgcg tttgttgatg aaggctgtta tggagaaaag ggcttagaca
    18901 gcgtgagatc gggtatgaaa aaagaggcta ttctttttta tctagactct gttggggcag
    18961 atacaccgct acagttttcg ggaaattatt tttcaaataa agaacagtgg ctgaaacaag
    19021 ttgataagtt gaaagaaaaa aacgtcaatt atatttttag tgcaagaaaa aagcaagcgc
    19081 agttcttcta tttgaccaaa acagatttac gagggaaaac atttaattgg caaaatgcca
    19141 atcagattat tgctcttttt cgataagtaa aaagtgcaaa acaaggagtg agaagactaa
    19201 tgttggaaat catgatcgtt acacatggta agttgagcga aggactgaaa gatgcggcgg
    19261 aagtaattgt cgggacatcg agccatattg aaacaatgtg cttagagtct ggggaggaca
    19321 tccaaacatt cggagagaag gtcaaagaaa aattaactat gatcaacaca gcagaaccca
    19381 tgctagtatt tgttgatcta attagcgcaa gtccgtacaa tcaagcgctc ttggcagtca
    19441 atgaactgac tgaagaaaag aaagcagcca cttatgtcat agggggcgtg aatctgccaa
    19501 tgttgttaga agcaatcaat catcagatgc ttcacacacc gatagaagaa gcggtggaac
    19561 aaattgtcaa acaaggaaaa gataatttaa ctgtttggca tgcttcagcg gaagaaaaaa
    19621 aattagatga ggacgatttt tagaaggaag gctgaaaaga tgaagtgggg atttagatgg
    19681 tatggtgaaa aaggggattc gatcccatta aaatatgttc gccaaatacc gggaataaat
    19741 gggataattg gaactttgct gactaaaatg cctggagatg tttgggagat ttcagaaatt
    19801 gaggagttga aagcatctgt tgagagccaa ggactggaac tgcttggaat tgaaagtgta
    19861 gcgatccacg atgcaatcaa ggcagggaca gatgaacgag attactatat tgataactat
    19921 atacgaacaa tcaaaaactt gtctcgatgt ggggttcgat tgatttgtta tagcttcaaa
    19981 ccaatttttg gatgggcgaa aactgatttg tttaatgaaa acaacgatgg aagctattct
    20041 cttttatacg atcagaaaat cgtcgatcag atggagccag gagaaatgta tcaattgata
    20101 cacagccagt caaagggatt caaattgccg ggttgggaag aagaacggtt gaaaaaattc
    20161 gctcagctgg aaaaactgta tgaaggaatg accgaagaag aattatttga aaatctgact
    20221 tattttttag aaaaagtgat tccagtgtgc gaaaaagcag atgtaaaaat ggctatccat
    20281 ccagatgatc cgccatggga actttttggt tttcctagag tgacgaaaaa tcttggcgac
    20341 ttgaagaaaa ttctaaatgc agtagattct ccatataatg gactaacgct gtgcacagga
    20401 tctttagggg caaatccgga aaacgattta gttgaaatca tccatgaggt aggttcgcgc
    20461 atccattttg tccacttcag aaacgtggag tttttaggag aaagaaagtt caaagaaacg
    20521 gcacatccct ctacagaagg ctcccttgat atgtatcata ttatgaaagc gttggtgcaa
    20581 gaagggttcg acggtcctat acgccctgat catggtagaa ccgtgtgggg tgaagtggct
    20641 atgcctggat atggcctgta tgatcgtgca atgggactca cttacatgca aggtctatat
    20701 gaggcgataa gtaaggaaaa accactggga gagcattatg aataaaatca agctcttcat
    20761 ctttgatatg gatggccttt tattagaaac tggacgttta gcttatcgtg cttatgtaaa
    20821 atcagcacaa aagtatgatt atgaaatgcg taaggaagtc tattatttac taactggcca
    20881 aacagaaatg gcgataagag aacaaatggg cattttgtac ggagaagatg ttccatatat
    20941 caaatggcga gaagcaatca atcaatataa agaaaaaatc gtgaaagaag ataagagagt
    21001 atatacgaaa aaaggagcag aagaaatttt atcttttgca aaagaaagag gaatccatac
    21061 aatcgtggct tcgtctaata cacgggtaaa agtagaaatg tatttaaaaa tggaaaatct
    21121 atatgccttg tttgatcaaa tcatttcagg ggatgatgta aaaaaaggaa agccggaacc
    21181 agagatcttt ttgaaagctt gttcaaaaat gaatattcct ccaagtgaag cacttgtatt
    21241 tgaagactcg attgcaggga tcgaagcagc aaggcgagcg ggcattttgt cttttctcat
    21301 caaagatcat ttgatagggt tgcccgatca taatgggaag cataagttga aagtagatgt
    21361 aagtttatat gaatccaata aagcagacta tgaattttat gacctgcatc aagcaaaacg
    21421 atttttagaa agtaaagggt tgtatctcta ataaagagga ctattttcta gtcctcttta
    21481 tttattgtcc tgcacgactg agggaacaga tgaaaaagaa gtaatttttt attttgaagt
    21541 atctacctta tttgtagtga acaatttaga acaaacacaa aacgcatcta ctgttatcta
    21601 ccagtagatg cgtttaaaga gaagaacgag aatcgtccat atgagggttt ccactcctta
    21661 tgcagatttt tttaattctt gcactgcttg ttcgatcgta acacctgtcg ctacatgatt
    21721 ggtattttct ttgtcaaaaa cgacaaatag attcaaatct gtatccaagg ttactcgata
    21781 ttgtatatct ttgttataga atgtcatagc cattctcctt tctttaaata cttgctgaaa
    21841 ggcaacgaac gcgttgataa cgaaattcca tttataagta taccaccaat tgaaaggttt
    21901 tcatagttaa gaaattttaa agtcaaaagt tgcagataaa agcaaataaa caagaaaaca
    21961 aaaagtgaat caatcgcggt tattcaactt tttcttcgat tcgaagacaa tagaagacaa
    22021 tgaattttat atttcattgc attatccttg tgttattaga aaaagcatga tatattaaat
    22081 gagtaatcta ttttgaaacg taatttgagc gatatatcta catagataaa actccttttg
    22141 cctatcaaat atttattgca acgaaaaaac gtacattgat acgtatcagg aggaatttat
    22201 ttatgaataa cggtacagta aaatggttca acgcagacaa aggttttggc tttatcactg
    22261 gtgaagatgg aaatgatgta tttgcacatt tttcagctat ccaaggtgac ggcttcaaaa
    22321 ctttagaaga aggccaagca gtgtcttacg atgttgaaga tggtcaacgt ggccctcaag
    22381 caacaaacat tgttaaagct taataaaaat tgacttttaa ccgtcttttc gaaagaaaag
    22441 acggtttttt tgtacgaaaa aatt
//
LOCUS       DACKAC010000045        21781 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.045, whole genome shotgun sequence.
ACCESSION   DACKAC010000045 DACKAC010000000
VERSION     DACKAC010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.045"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(531..2027)
                     /gene="lysS"
                     /locus_tag="I0B46_001800"
     CDS             complement(531..2027)
                     /gene="lysS"
                     /locus_tag="I0B46_001800"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAR2084096.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(2138..3142)
                     /gene="dusB"
                     /locus_tag="I0B46_001801"
     CDS             complement(2138..3142)
                     /gene="dusB"
                     /locus_tag="I0B46_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAR2084097.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(3143..4030)
                     /gene="hslO"
                     /locus_tag="I0B46_001802"
     CDS             complement(3143..4030)
                     /gene="hslO"
                     /locus_tag="I0B46_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAR2084098.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(4247..6358)
                     /gene="hflB"
                     /locus_tag="I0B46_001803"
     CDS             complement(4247..6358)
                     /gene="hflB"
                     /locus_tag="I0B46_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAR2084099.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6448..6993)
                     /gene="hpt"
                     /locus_tag="I0B46_001804"
     CDS             complement(6448..6993)
                     /gene="hpt"
                     /locus_tag="I0B46_001804"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAR2084100.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6993..8438)
                     /gene="tilS"
                     /locus_tag="I0B46_001805"
     CDS             complement(6993..8438)
                     /gene="tilS"
                     /locus_tag="I0B46_001805"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAR2084101.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8558..9028)
                     /locus_tag="I0B46_001806"
     CDS             complement(8558..9028)
                     /locus_tag="I0B46_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAR2084102.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(9074..9499)
                     /locus_tag="I0B46_001807"
     CDS             complement(9074..9499)
                     /locus_tag="I0B46_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAR2084103.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9566..9835)
                     /locus_tag="I0B46_001808"
     CDS             complement(9566..9835)
                     /locus_tag="I0B46_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAR2084104.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9846..11441)
                     /locus_tag="I0B46_001809"
     CDS             complement(9846..11441)
                     /locus_tag="I0B46_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAR2084105.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11456..14977)
                     /gene="mfd"
                     /locus_tag="I0B46_001810"
     CDS             complement(11456..14977)
                     /gene="mfd"
                     /locus_tag="I0B46_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAR2084106.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14994..15554)
                     /locus_tag="I0B46_001811"
     CDS             complement(14994..15554)
                     /locus_tag="I0B46_001811"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAR2084107.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15907..16881
                     /locus_tag="I0B46_001812"
     CDS             15907..16881
                     /locus_tag="I0B46_001812"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAR2084108.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            17051..18451
                     /locus_tag="I0B46_001813"
     CDS             17051..18451
                     /locus_tag="I0B46_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAR2084109.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18529..19251)
                     /locus_tag="I0B46_001814"
     CDS             complement(18529..19251)
                     /locus_tag="I0B46_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAR2084110.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19330..19836)
                     /locus_tag="I0B46_001815"
     CDS             complement(19330..19836)
                     /locus_tag="I0B46_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAR2084111.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19959..21758
                     /locus_tag="I0B46_001816"
     CDS             19959..21758
                     /locus_tag="I0B46_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAR2084112.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WEEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
BASE COUNT         6043 a         4747 c         3904 g         7087 t
ORIGIN      
        1 agagtgtttc ccgattcatt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagcgattgt caactctttt ttaaaagttt ttttcgattt cgtttttcga aatctttcgg
      121 caactcattt atcataactt atcgtttgtt atttgtcaag aacttttttg aagttttttt
      181 cgatagaatc aagcttcgca agtggttctt ttttaacgac tcagttatct tatcatgtcg
      241 ttagtaacat gtcaataact tttttgttat gtttttgaat aaatattttc ttctgtcttt
      301 tggacaagta ataatatacc aactaaattt agcttggtca acgaattttt tgtatttttt
      361 caagtttttt tcaataaacg attattcata acttttcttt ctttatcgtt cgttttcgtc
      421 ttctttaatg tctagaatag tcggcattag cttttgaact ttcgttatta tttattcctt
      481 tctacacagc aaaagctgcc taaggaattc cttagacagc ttttattcga ttatctcatt
      541 gttgggaata gcaaaacatc ccgaattgat tgtgcatcag ttagcaacat tactaatctg
      601 tcgatcccga tgcctagacc accagttggc ggcataccgt attctaaggc ttccaagaag
      661 tcttcgtcta caccgtgagc ttcgtcattt cctagttcac gttctttttc ttgcgcttcg
      721 aatctttctc tttgatcaat tggatcgttc agttctgtaa aggcattagc aaattcgcgc
      781 cccactatga atagttcgaa tcgatctgtg aatcgaggat cttctggatt tttcttcgct
      841 aaaggcgaaa cttcgactgg atgtccgtat acaaacgtcg gttgtgtcaa tgtttcttca
      901 acaaatgttt caaagaattc attgataaca tgtccgacac ccatagcttc ggttacttct
      961 acattgtgtt tttcggctaa agctaatgct tcttccaatg tcatttcttg ccagaagtct
     1021 acaccagttt gttctttgat tgcatctacc atatggatgc gtttaaagtc actttctaag
     1081 tcgacagctt gtccatcata tgtgattttt gctgttccta gtactttttc agctgcgtta
     1141 cggataatac cttctgtcag attcattaca tcattgaaat cagtatatgc tgtgtatgct
     1201 tctaacatcg taaattctgg attatgtgta gtatcaattc cttcattacg gaaaacgcgg
     1261 ccgatttcat aaactttttc cattccgcca acaattagtc gttttaaatg aagttctagc
     1321 gcaatacgca aatataaatc aatgtccagt gcattatgat gcgtgataaa tgggcgtgcg
     1381 gcagctccac ctgcctcatt gtgcaatact ggtgtttcta cttcaatata gccattgcca
     1441 tctaaatagc gacggatttc gctgataatt tggcttcgtt tcataaatcg gtcaaagctt
     1501 tcgcgattac tgattaaatc caagtagcgt tgtctgtagc gttgttcaat attcgttaag
     1561 ccatggtatt tatctggcag tggacgaagt gcttttgaca aaatcgtgat ttctgaagct
     1621 ttgatagaaa cttcgcctgt gtctgttttc atgatttgtc cagttacacc aaagaaatct
     1681 ccaaggtctg catgtttgaa tacttcatag gcctcatcac ctacttggtc ttttcttaca
     1741 taaatttgaa tttggccttc acgatcttgt agatgcgcaa atccagcttt cccttttcct
     1801 cggcgagtca tcatacgacc agctacacta gcggtcaatc ccatttctgc tagttcttct
     1861 tttgttcttg gatcaaatag ttcatgcaat tcttctgagt tgtgcgtgcg ttcaaatcgt
     1921 tttccaaatg gatcgattcc atcttcacgc aattgttcca ttttttgacg acgtacaagc
     1981 atttgatcat ttaaatcttc tacttgatgt tgttgttcct gtgtcacaaa agttcctcca
     2041 ttctcatgtt aatctctttc ctataatgcc aatgaaacac aaaaaaagca agccgaactt
     2101 ggaatttcgg cttgaaaatt cacgaaaaac ttagaaatca caacgtttgt ttcaatagta
     2161 ctttttccgc tttttctaca aattgatcca gcaggtcatt gatttcttgc tgtttttctg
     2221 tctgattgat tgctactttc actttcgctg ctcgaggaat cccttttaaa taataagccg
     2281 catgtgttct gaattctcta caagctactt tttctccttt gagatctgct aagcgttgta
     2341 aatgaagttt agctgtagca attttttcgg ctggtgttgg ttcaggaatc agttctcctg
     2401 tttcgagata atgctgtgtg cggtgaatca tccatggatt gcccagcgct gcacgaccaa
     2461 tcatcacacc gtctgcaccg acatagtcaa gcatccattt tgcatcttct ggcgttttga
     2521 catccccgtt ccccataaat gggatcgtca aatgtttttt cacttctttc aatacttccc
     2581 agtccgcttt tccttcatac atctgaaccc gtgtacgtcc atgcatagcg atggcacttg
     2641 ctccagctga ttgtgctgct aatgcatttt ctacagcaaa gacgtgctct tcatcccaac
     2701 caatacgcat cttgattgtt actgggatat ttactgctga agaaacagct tggaccattt
     2761 cgtatacttt ctcaggatct aaaagccatt ttgctcctgc ttctgctttg attactttat
     2821 ttactggaca gcccatattg atatctatta tatcggcagc tgtgttttct tcgacaaatt
     2881 gagcagcttc aactagacta tctttatttc cgccaaatat ctgtaaactt aatggatgtt
     2941 ctgtttcttc aatgtggagc atctctaaag tttttttgtt tctgaaatga ataccttggt
     3001 cactgatcat ttcacatacg actaagcccg caccaaattc tttgacagtg actcgaaatg
     3061 ctgaatttgt tattccagcc atcggtgcaa caacgacacg attagggatt tccacattgc
     3121 caattttcca tgtgtgttcc acttaaatcc ctcctaaggc ttctttcttc aatccatcta
     3181 attcttcttc gctatacttg tatttgttat tacagaaaga acaaaccgct tctgcaccgt
     3241 gatcttcgtc tatcattgct tggatttctt ctgcacctaa cgcgataatt gcttctgcaa
     3301 atttttcttt tgaacagtca catttgaatt gaacaggcat tttttccaaa atgttcaatt
     3361 cttctgtacc aagcaatcct ttcaatattt cttctggtgt ctgtccttca tctaagaggg
     3421 tagagattct tggtgtcgtt tttaattgtt cttcgatctt gctgattgtt tcttcatcgg
     3481 cacctggcat gatttggatc atgaatcctc ccgccgtctt gatgctgtcg tcagtattga
     3541 ccaatacact cagtccaaca gctgaaggga tttgttctga gatagcaaga tagtaagtaa
     3601 aatcttcagc gatttctcca gaaacaatcg gcgtttgccc agaaaaaggt tcttttaatc
     3661 ctaagtcttt tgtgacagta aacattcctt gtgttccaac tgctccccgc acatcgattt
     3721 taccttcact atttagagga agactgagat gcggattttt gatgtaacct ttcacatctc
     3781 cctttccatt tgcatctact acaattcctg ttgctggacc atcgccttgg attcgaacag
     3841 ttagtttgtc ttctcctttg agcgtcgcac caagtagcag actaccgacc aatgtccgtc
     3901 ccaaagcagc tgaggaggtg tgccaagtat catgacgtct ttgtgcctcc gctacggtat
     3961 ttgttgcttg aacggcgtat gctcggacga atccgtcgtg agcaagtgcc ttaaccaaat
     4021 aatctttcat ttgttatgta ctcctttttc cgtttttaaa ctcatagtga atcatactag
     4081 caactactgc ttttttcaac tttggaatgc ttcttttaaa aataattcag aatcatctag
     4141 aaaacaggac atcaccctat ttacaactta aataaagaaa tctcccgaaa accggttttt
     4201 gttccggttt tcgggagtca tatcagatca attgacctat atttttttat ttgtattgat
     4261 cgtctgaatg attatcttct gattgttgag aatctgaatc atgttctgct ttattttcct
     4321 cattcagttc atgttttgct tcttcaaaat cacgtttttc ttccgcttgt ttttcagcgt
     4381 ctttttcttc caaagcacgt tttgcttctt caaaagtttt cgcttctttt tcgcttggat
     4441 aatcatttga ctcagtacct tcaggcatca caccgtgttc aaacaatgac ttgattgctt
     4501 tggcatccaa tgtttcgaat tccaaaagtt tttcagcaat cagtttatgc tgtgcacgat
     4561 gagcttcgat gatttctcga gctttatcat gagcttccat caagattttt cgtacttctt
     4621 gatcaatctc gaaagcgact tgttccgagt aagcttttgt ttgaccatag tcgcggccaa
     4681 caaacacttg atggtttcct tcatattgaa caggacctaa acgatcactc attccatatt
     4741 ctgttaccat gcttcgtgct agagctgtcg cttgctcaaa gtcattggaa gcaccagttg
     4801 attggacatt gaagatgatt tcttcagctg tacgtccacc aagtaagcct acgatttgtt
     4861 caaacatatc ttctttcgtc atcaagaact gatcttcttt tggtaaagcg atcatatatc
     4921 cgccggcgcg tccgcgaggg atgatcgtta ctttgtggac gacccgagcg cgactcaata
     4981 ctaatccgac aatcgtatga ccagcttcgt gataagcaac catttcgcgt tcttttttgc
     5041 tgattacgcg atctttttta gctggacctg caatcacgcg atcttccgct tcatcgatat
     5101 cagaagcatc gattttcttt ttatttcttc tagcagctac taacgctgct tcattcaaga
     5161 cattttctaa atctgctccg gcaaaacctg gtgtctgttg agccacaact ttcaagtcaa
     5221 cgtcatctgc catcggttta ttccgtgcat ggactcgcaa gattgcttca cgacctttta
     5281 catctggacg tccaactaaa atctggcggt caaaacggcc tggacgtagt aacgctgggt
     5341 ctagtacatc agaacggttg gttgcagcaa tgacgattac tccttcattt ccgtcgaagc
     5401 catccatttc tacaagcaat tggttcaatg tttgttcgcg ttcatcatgt ccgccgccca
     5461 taccggcacc acgttgacga cctaccgcat caatttcatc aatgaaaatg atagctggtg
     5521 cgtttttctt cgctgtttca aacaaatcac gaacacgact tgctccaaca ccgacaaaca
     5581 tttctacgaa atcagaaccc gagatcgagt aaaacggtac tccagcttct ccggcaacag
     5641 cttttgctaa taaggtttta cctgttcctg gaggtccttc taaaagtaca cctgccggga
     5701 tacgggcacc taattcaaca aatcggcgag gatctttcag aaattcaaca acttcaacta
     5761 gttcttgttt ttcttcttcc gcacccgcta catcagaaaa acgtacgcga ttagcttttt
     5821 tgtctgcttc tttggctttt gatttaccaa agttcatgac acggccgcca ccgccaccgc
     5881 cgccgccttg ttgtcccatc atcatgtaaa agaaaaagat gatgattacg attggcaaga
     5941 agctaagcaa tagggaaacc catataccgc tgcttgattc ttcttttact gttgtagcaa
     6001 ctttgttttc ttttgccagt tctgttactt ggctcaatgt tgtatcattt ggcaaaatga
     6061 ttgttgtgaa atgggtagta gtcgtatctg ttgaacctaa gatagctaac ccaccgctgt
     6121 tagcgacggt ctgctgttct ttgtattcac cagtgatctt atatacacca tttgcaggct
     6181 gcacttcaaa ttctttgatc ttgcctgctt gtagttgttc tgtgaatttt gagtactcga
     6241 tatctgacga tcgctggccg ttattaccaa aaatgaaata aacgaccatt accatcgcta
     6301 agattacgag gacataatac aaggcatttt tcatcatgcc gttgttcttt ttgttcatag
     6361 ttgccctccc taagcgtcaa tttaatttaa cagtttttta tcaatcctga ccattctatt
     6421 gtaccataat ttaattgaga tacagcatta atttgattga taaacactag gtttcaatac
     6481 accgatgtac ggaaggttac gatatgcttc tgcataatcc aagccataac cgaccacaaa
     6541 ttcatttggt acgttgaagc caacataatc tgcttcgata tttactactc ttccttcagg
     6601 tttgtcaagt agtgtaacaa ttttgactga ttttgctttg cggtatttga aaagatccac
     6661 taaataagca agtgttcttc cgctgtcaat gatatcttca acgatcaaca gatcgcgacc
     6721 ttctacattc gtatctaagt ctttgacgat ttttacttcc cctgacgaga ctgttgcatt
     6781 cccgtagctt gaaacatcca tgaaatccaa ttccaaatac gtatcgatag aacgaacgat
     6841 atctgccatg aatggtaccg ctccttttaa gacacctact actaggggat tggtgttttg
     6901 gtaaatttct gttaactctt tgcctagttc tttgcaacga acttgtatct cttcttgtga
     6961 gatcaaaatt ctctcaatat ccttttccaa catcaggttc tccttccaat tgcttctttt
     7021 tgatatttgt aaagaagtat gtagtgtatt ttatcagttt cttcacgaat actcaaatat
     7081 gaaaaggcaa aaggtatcaa gctatatata tttcttttgg catctgtaat gatccaagca
     7141 tgctgccttt gtgaattagg tactttctga tcgataaaat atctagatac tttttttgtg
     7201 agattctctg ttaatttaat gcggtctcct gcttttcttt tttcgacata cagttgcaca
     7261 tcagcaggaa tccaaatgtc atgagaaaat agagaccatg aatctgttgc tttgatgtcc
     7321 cctgcatttt caggtttgac cagaccaatc cattctgttt cattcaaata tattccttgt
     7381 tctggaatca gcaacaagcg ctggtcttct gtctggctag ttgcttgttt ttctttttta
     7441 gagagtacaa caaaatgata ctctcttttc agtatccaat tattgccaat actgagttgc
     7501 cattggcttt ttttctgttg ccactggtct aaaatcgact ccatttgttg ttctttcaaa
     7561 ataattcctg tttttgagaa tgctttgtcg aaaaaggagt aaaaaaaata gtatcgttca
     7621 gcatctgtca tttcttcaat catttctgca gaaaatcgaa attgttcctc ccactgttcg
     7681 acttcctttt cgatcaactg tcccatatat ttttgaatga cttggtctgc ccattggatc
     7741 tgttgcgaaa attgctggaa atgtctcaac gcctgtgtat tttcttgttt caattgagga
     7801 actaccaaat gacgaagccg atttctctgg atatctaaca attggtttgt ctgatcctca
     7861 aagtaaacaa gctgcgattc cgctgcataa gtatataggt cttctttgct aaaactcagc
     7921 aacggacgaa tcagtctgcc cgtagcaaat ggttgtactt ctttgattcc agaaaaagta
     7981 tttaactgtc ccccacgaac gattttcatt aacaccgtct cgatttgatc atctccgtga
     8041 tgagcagtca tcaacgtgtc atattgtcct tcttccatca cgcgagtgaa ttcttgataa
     8101 cgaaagttac gagcttctgt ttctatggcg gatttggctg gatcttccca aatacttaca
     8161 tataaaggga gattcttctc ttcacaatat gagcgaagat aggcagcttc ttctgctgat
     8221 tcttctctta attggtgatt gatgtggacg atacctaaag aaaaaccgat ttggcgttgc
     8281 acttgtaaaa gtaaatggag caaaaccatc gaatcaacac cacctgatac cgctgctaaa
     8341 attctcgatc cttgttgcca gaatccttgt tcattcccgt gccgaataaa tgactgctgt
     8401 aatagcggtg cttttttccc gcctttttca acgttcatcc tttccacacc ctttcttcct
     8461 aaaaaaaaga gagcgggaaa acgtctaaaa cgacaattaa cctgccctct gttgatacta
     8521 tgacccaaat agtcgcacta tcattatttg cggcttttta gctgcgacgt ccgccacggc
     8581 caccgcgctt tccttctgtg tttcgcttca atgaagataa gcggtcatcg ctatctttca
     8641 aaaatgaact catcaatgaa tcgaagtctt ctttttgagc tgaaggttga ctgcgagaaa
     8701 aagatttttt ctgtgctgga cgatttcggt tgtcacgggg atctttttgt tgatattttt
     8761 taggacggct ttcttctcca gattcagcag gttgttcctg cgctttccgg atagatagtc
     8821 cgatttttcc atcatcattt actgaaataa ctttaactgt tacttcatct cctacactta
     8881 aaacatcatg gatgtctttt acatagccat ttgacacttc gctaatatga actagacccg
     8941 tttttccagc acctagatca ataaacgcac caaaatttgt gattcctgat acttttcctt
     9001 gcagttttgc tcctacctcg attgacataa aaaaaatgtt cctcctaatt atttcccttt
     9061 tttatattcc tgcttatttt gcagttgtag aactagttga ttgatctgtt tcatttgtcg
     9121 agctgtctgt atttgattct aatataggat aaactttttc accgtctttt gagtaaaaga
     9181 atttgcttct ggcaagttta gctacatact catcatcttt cagcaatgaa acatcttggt
     9241 gcagctgatc aacgtctgcg ttgacttctt tcatttcagt atttgcttcg actctcaatt
     9301 cgttcaattg atgcatcctt tgcatgtcat ggaaaatctg cacacctatt atcgcgaaaa
     9361 tgaccaaagc accaacaaaa attgcagcca gtctgcgtct tcgaaaaatc agttgacgtt
     9421 gttgttgctg aaattcagca tactttttct ttgcatattc cgtatctagc gcagcgattt
     9481 tattgctttt tttcgtcact tggattcgct ccaatctttt tgattgtctt tttcattata
     9541 ccaatatcgt gaaagcttgt ctaatctatt tctctagtgt ttctgtgcga ttttcagaaa
     9601 taatttcgta cattttttgt gcatcttctt ttttggttga ttcatgtaat tccgtaattt
     9661 tcacttccaa cgttttgttg ccaaactgga ctttcaaaat atcgcctact ttgacatcac
     9721 tggaagattt cgccaattta ccgttgattt gaattcttcc tttatcagct acttcttttg
     9781 ctactgatcg tcttttgatg attcgggaaa tttttaaaaa tttatctaat cgcatatctt
     9841 attcctcact ttttaatttt tttatttaat cgcaggattt tcttaccaaa aggcaacatt
     9901 agccattctc gtactgtaaa cagacgaaga cgaatcactg cataaatgaa cacagaagcc
     9961 ccgatcccaa ctcctatcac gctgacaaaa aaagcagtgg aacgatgagt tacaggacca
    10021 aatactatat tcaatacacc ataatacagc agcagtacta ctgtcataca aagaaggctg
    10081 aacatcaact tccgtccaaa atgccgttct ttccaaaaag catttagctc ttttgtttca
    10141 attcgaacaa aataccaaag cgtcacacct aatcctaaaa gggtagacag gcttgcacct
    10201 gctgttccta ataaaccggt cagaaaagaa gtagtcaatc cttttaccaa tagtccccat
    10261 cctgcgcctt ttaaagaagg gcgaaacgag ttcttgcttt gagcaatact ttgataagct
    10321 tggatgactg cagtgaaagc aatggaaaaa acaaataata caagcgctgc atttcccgca
    10381 taatctttaa acaacgcata attgatataa gggagcagca aggctaatcc caaagacgca
    10441 gctaatgcta aagcggtcgt caatctaaga tagattttgg ctgtctgtaa aaactgacga
    10501 tagatccggt tggtcaaatg acgagtcaac gcaggcagga atgtcgcact taatgcagtg
    10561 gcaatcacta aacctaattg tactaaaggc tgacctcgat catagacccc ttttgctatc
    10621 ttcgcaccgt attctgacag tccgtaaact tccaaagcat tttttacaaa gaaagagtcg
    10681 atcagctgga aaaagataag caatccacta taaatcgaaa ccagtccgcc ttctaccaga
    10741 aaacgccgga tcaagggctt tgacggctgt ttgaacaatg gaaaccgtcg gaagcttaac
    10801 gctcctccat gaatcttttg ttcatagtag tgaaggatcc catatgcgca taaaccgcca
    10861 aaaacagctc cactcattgc tgccgtccct gtttggtaca ccgtccatcc aagctttcta
    10921 aaagcaaacg cactacagac aatcacaaaa acacgaacga actgctcaac tacttgagaa
    10981 acagcagttg gttccatcaa gaatcttcct tggaaattcc ctcgataaaa agatagacca
    11041 ggcatcagca aaaaggtaaa ggaaactact tggatcagcg gtttcaattg tatgtctccc
    11101 atcatccaag caatccagct gctaaatgca aagacaaaca accacaaaaa gacacctgtc
    11161 caaaatacta agggataaag attttgcaaa gcttgctttc gctcgaaagg attctttctt
    11221 tctgcaacgt actttgatat gaactgcgga agaccggata aagctaaagt cattgctatc
    11281 ccataaattg gataaacttg ctgataaaca taaaaacctt cgtcgccgac taagttttgg
    11341 aggggaattc ggtaaagggc acttaatact tttgctatga aagaagcaat cgtcaataca
    11401 aatgctcctt gcatgacgcg ccgcatttct ttatgatcca aatcattgct ccttcctacg
    11461 ctgacagata tttttgctca cgcaacgctt tgacaaactt ttggatttcc tgaagccata
    11521 ctgcttcttc catttggttg gggatcgtca agcgtatcac catctgttct ttttcaacgc
    11581 ctagactggc ttttagtttt gtcgctgtca gtgcttcgaa taactgttcc acactatagc
    11641 gttttgttcc aactttactt agagtaaaga ctatatgcgc ctcctgtttc cttatcgttt
    11701 ctaggagcgc tcgatcgcca tccattttga tctgcccaat tgtcaagaga tgtgctactt
    11761 cttctggata ttctccaaaa cggtcgagca gatcatcttc tagttcatct agcatttctt
    11821 gcgaatcaag ttcacgaatt cgtttataga tctcgatttt ctgccgttga tctgccacgt
    11881 atgtttcagg tagatacgca tcgacaccga ggtcgatctc aacagtggtc ttctccactt
    11941 gactattctt tccttgttta cgagaaacag cttcgcttag catttgagaa tacatatcga
    12001 aaccgactgc atcaataaat ccatgctgct gagcacctaa gagattacct gcaccgcgaa
    12061 tggaaagatc ccgcatcgca atcttgaatc cagatcccag ttctgtaaaa tctttgattg
    12121 cctgaagacg tttttcactg acttcgttaa ggatcttctg ctgctcatac ataaagtaag
    12181 catatgcgac acggctgctt cttcctaccc gccctcgcaa ttggtataac gtggataatc
    12241 ccatataatc agcgttctca acgaacaacg tatttgcgtt cgggatatct actcctgttt
    12301 caatgatggt tgtagtgaca agaacatcat attgaccttc aataaattca aaaagcgtat
    12361 tttctaattg cgcctctgtc atttgcccat gagcataacc gatccgtgct tccggaacga
    12421 gttcttggat ttcttctact ttttgttcga tcgtttctac ccgattatat aaatagaata
    12481 cttgtccacc tcgtcccatt tctcgatgga ttgcttcgcg aatagcccca ggattttttt
    12541 ccataacata cgtttggatc ggataacgat tcgcaggcgg tgtttcgatt actgaaagat
    12601 cccgcacgcc taacatcgac atatgcaaag ttctcggaat cggtgttgca gtcagagtca
    12661 aaacatccac ttgtgcacgt aactgtttca atcgttcctt atgttttaca ccaaaacgtt
    12721 gttcttcgtc aatgatcagt agacccaaat cgctaaattc aatatctttt gacaaaattc
    12781 ggtgggttcc tacaacaata tcaacttgac ctgtacgcag ttgttcgatg gtttcttttt
    12841 gttgctttct tgttctgaaa cgacttaata gcccgatatt tacggggaaa ccttcaaatc
    12901 tttccagcat cgtttcatag tgctgctgtg ccaagatcgt tgttggaact aaaaaggcta
    12961 cttgtttgct ttcctttatc gctttaaagg ccgctcgaag cgctacttct gttttcccat
    13021 aaccaacatc acctacgagt agtcgatcca ttggtttttc tttttccata tcccgtttga
    13081 tctccgctgc acttctcaac tggtcatcag tttcagagta ggggaaggca ttttcgaatt
    13141 ctttttggta ggcgtcatca ggtccaaaag catatccttt ttctgactct cttgcggcat
    13201 ataatttgat tagatcgtca gcgatatcct cgatttttga agagactttt cgcttggttt
    13261 tcgtccattc acttccgcct agcttattga tgcgaggcgc tttggcttca gaggcgacat
    13321 atttttggat cagattcagt tgagtaacag gaataaacag tttgtcgtcg ttttgataca
    13381 aaatagtcat atagtcttgg tgaacgccat ctacttctag cgtttccatc ccaatgtatt
    13441 tcccgatacc atgattggca tggacaacgt aatctcctgc ttttaactca ttatagcttt
    13501 tcaatcgctc tgcgtttgtt actgtttgac gtctcgtttg ttttttcgtc gttttttgga
    13561 aaatttcttt ttctgtgata gctactattt tttcttgagg aagttcaaaa ccagcctgca
    13621 aagctccttc aactaattgt acctgtcctc tgatcaatgc atttttttct gtttctacta
    13681 ctaatatgtc ttcatctcga agcatttgtt ctgctttgcg tattctgtcc tttgtcggaa
    13741 taaagatcac aacagtctgt ttttgcttgc gccaacggtc catttctgtt ttcagcaatg
    13801 gcatttgtcc gaagaactgt tgcatcgtcc gatattgaaa attatggatt gcttggaaac
    13861 gaagatttcc catccctttt tggaagagtg agaaaaaggt tgtggtgaac ttttccttct
    13921 gcacgatttt ttgtatatct gctgaaaatg tctgctcaga aaacacccgc aattcgctga
    13981 ttttttgagt ctgccattct gccgcttccc tttcgatttc ccgatttgtt tccatgattc
    14041 gctgataatc atcaacgact agtaaactat ttgaagggaa ataatccaag atcgtcacag
    14101 attcttggta aagaagatct gagtagaatt ttgcttcttc tgtcgggaca cctgcttgcc
    14161 agcttgaagt cagctgaccg aagtaatctt gcaaaaactc gcggtctgct tcttctgtgg
    14221 tcgcagcaag ccgtttttct agcatttttt gcatttgctc tattccatgt tctagatcct
    14281 ctggtccgta gacttgttca ctcgttgggg aaacccagat ttcttccgtt ttttcgattg
    14341 agcgttgtgt ttctgcttca aaataccgca tagaatctat ctctacatca aatagttcta
    14401 cacgaacagg ataggttgtc gttaacgggt aaatatcgat aatgcttcct cgaatactga
    14461 attctcccgg ttttgcgacc atctcttctc gtacatagcc catcaaagtc aattggcgag
    14521 gaagattttc cacgtctatc tcatcaccaa tatgccaatt caattgcgcc tgtagccaag
    14581 ttttcttatt cggcagtcgt ttgtgcaaag atgcgactgg caacaaatag atacctgctt
    14641 ttttttcttg aagtgcgtta agcgttgcaa ctctctcgcc tcgcgcttct ggagaagaaa
    14701 aggccatttc agcagaaagc acttcatcca ctggaaataa atggatctga tcttgcggaa
    14761 tcacatgttg cagatcttct gctaactgat tcgcataata caaatttggc atcagaataa
    14821 tcatgttttt atttgttttc ttaaacgctc gagcaaatag aagtgttttc gctgagcccg
    14881 ctaatcctgt gattagttgt ctaggcattt tttcttctaa attgctgacc cattctttta
    14941 cttgcggatc ttttccgact aattgaataa tatccattcc ttttcctcct tcctcaattg
    15001 aatcgattca tcgcatccac gaacgaatgc ccttctatga gatattcgac tgctttaaca
    15061 gattgacaca tgctttcttc tatcaaaggc tgattttctt tgctgaaagg actaagcaca
    15121 tgctggacta ctgttttttt cccttctggc ctgccgattc ccaccttgat tcgatcaaag
    15181 acattcgtat tcaaatggga aataatactt ttgataccgt tatgcccgcc ggcacttccc
    15241 ttttgacgga gccggatttt tcccactgcc agatccaaat catcgtagat tacgactaat
    15301 tcctctggat aaatcccaaa ataagtcatt agtgggccga ctgcacgacc agattcattc
    15361 atgaatgttt gtggtttgac taaaaggatc ttttctccat tatgaaaaaa ttcggcaact
    15421 tctgcttcga acggattttt tttgaaagta gcttgatgct cgcgtgcaat tttatccacg
    15481 accataaagc cgatattgtg ttttgtatat tggtatttgg tacctgggtt tcctaacccg
    15541 acgatcattt tcatttttgt tttccaccta attctaaaaa gcagcacaaa aggctggact
    15601 gctaattagc ggtccagtcc atcgagcttt tttctttttt atacttgtca atgcatagta
    15661 taacacaaaa caaggcgttt cggctaaaag agaatcgtta ataaatgata aataaattaa
    15721 aaatttcttc aaagtttaat gaaataattg atatataaaa atagtgaaat aacgcacatg
    15781 taagctttta catataatac aaccttgtta tctgttatat tttatagcca aatagcgtga
    15841 catggggctt gagaaatgtt agcataagca tgggaaaaaa caaatcaatg gaaggattga
    15901 tactaaatga ctgcagctgc agaaaaaaaa gatcatcaaa aagttattct tgttggagac
    15961 ggcgccgtag gttctagcta tgcctttgct ctagttactc aaaatatcgc tcaagaagta
    16021 ggtattgtgg atatcaatac tgctaaaact gaaggagacg caattgactt gtctcacgct
    16081 ttggcattta cttcacctaa aaaaatctat tctgctactt acgcagacgc acatgatgct
    16141 gacttagttg ttattacagc tggcgcacca caaaaaccag gtgaaactcg tttagactta
    16201 gtaaacaaaa acctaaaaat caaccgcgat gttgtcacac aaatcgttga atcaggattt
    16261 aacggtatct ttttagttgc cgctaaccca gtagatattt tgacttactc aacttggaaa
    16321 ttctcaggtt tccctaaaga acgcgtaatc ggttcaggta cctctcttga ctcagctcgt
    16381 ttccgtcaag cacttgcgga attagttgac gttgatgcac gtaacgtcca tgcttacatc
    16441 ttaggtgaac acggagattc agaattccca gtttggtcac atgcaaatgt tgccggcttg
    16501 caaatctatg aatgggtaaa aaacaaccca gaaatcgatg aagaagctat ggttaaccta
    16561 ttctttggcg tacgtgacgc tgcttacaca attatcgaga aaaaaggcgc tactttctat
    16621 ggtatcgcag tagcattagc acgtatcact cgtgccatct tagatgatga aaatgctgtt
    16681 cttcctttat cagtattcat gaatggcgaa tatggattga acgacatcta cattggtgca
    16741 cctgctgtta tcaaccgtca aggtatccaa aaagtaatcg aaattccttt aagtgacagc
    16801 gagcaagacc gtatggctgc ttctgctaag caattaaaag aaattctaga tgaagctttt
    16861 gctaaactag acgctgaata atctttgaac atgacggaaa acatctattc ggtgttttcc
    16921 gtttttttat tttatcaccc ttatggtttc taaatttttt cataaagtag agataacctt
    16981 cgtcttttcc tctaaattat gctatcatga gaccgttcca ccctaaactt ttacttaagg
    17041 agttgtcgat atgacaagat cttcgcatcg aaaaaaatcg aaacatgttg ctttgctttc
    17101 agttcttggt gttctcttag tgctgatcag cttttatacc tatcgcagta catactacac
    17161 aaagcatttt ttgccgaata cagaaataaa tggaataaat gtcagcaatc taacagtcga
    17221 aaaagcaaat gaaaaattaa aagaagctta ttctgataaa aaactggcta ttaaagaaga
    17281 tggaaaagtc tggcaggaag taccaaaatc cgagctcggc tacaaagatg atttcacttc
    17341 ggagctttct ccgatcctaa atgaacaaaa cggatggact tggggaatga cttatgtctc
    17401 agcggcagaa aaacaagaaa tagatcctgt cagtcaaaac caccaaaaac tagacactac
    17461 cgttcagaca ttgacaacta agctgactga tttgaacaaa gaccggacac ctactactga
    17521 tgcaacgatc gaaaaagcag gtgactcttt cgttatcaag cctgaagtca atggaaatac
    17581 gatcgacgtc gatgctgcgg tcaaacaatt aaaaagtgcg gtaaactctg gtaaagatac
    17641 aattgaattg actgaattca aagaaaaacc aaaagtaaca tctgaagaca gctcactgaa
    17701 agaacaactc gcatccatga atgctatcgc aaatgtaaaa gctacttaca gtataaacgg
    17761 tgagactttc cagatcccat caagtgatat tatgagttgg ctgacctaca atgatggaaa
    17821 agtcgattta gatacagaac aagtacgtca atatgtaaca gatttaggca caaagtataa
    17881 cacaagtacg aatgacacaa aatttaaaag tacaaaaaga ggagaagtta cggtcccagt
    17941 tggcacttac agttggacga tccaaacaga cagtgagaca gaagcattga aaaaagcgat
    18001 tctggcaggc caagatttta ctcgttcacc cattgtgcaa ggtggcacaa ccgctgatca
    18061 tccattgatc gaagatacgt atatcgaagt tgatctggag aaccagcata tgtggtatta
    18121 caaagacgga aaagtggccc ttgaaacaga tatcgtttcc ggaaagccaa ctacacctac
    18181 acctgctgga gtcttctatg tttggaataa agaagaagat gcaactttaa aaggaacaaa
    18241 tgacgatgga acaccttacg agagtccggt aaactactgg atgccgattg actggacagg
    18301 tgttggtatc cacgattcag actggcaacc tgaatacggt ggagacttat ggaaaacccg
    18361 cggttcacac ggctgcatca acaccccacc aagtgtaatg aaggaattat tcgggatggt
    18421 cgaaaaagga acgcctgtat tggtcttcta acaaaaataa tatataaaaa gaaagcaccc
    18481 gtacgattgg tacaggtgct ttctttttat tatctaacaa tgtgctactt acatttcagc
    18541 agcagtaaag acttctgaaa catcatcatc gtcttctagc ttatctacta gacgttccaa
    18601 ttgctctttt tgttcctcat ttaaatcaat cgtcgtttgt ggaatcatcg tcaattctgc
    18661 ttgcgccaaa gtgaactctt tttccaactc atctcgtaca gcagcaaaat cttctggtgc
    18721 agtataaatc tcgaatactt caggagaagt ttgcagatct tctgcccccg cctctaatac
    18781 atcctcaaac atcgtctctt catcaacagt caacccatcg cgttcaatag cgatatatcc
    18841 tttgcggtca aacaagtaac tgactgaacc cgtttctccc agtgatccac cgtttcgagt
    18901 aaaagctact cggacatttg tcgctgtccg attacggtta tctgtcaatg catggactaa
    18961 gattcctact ccacccggac catatccttc atacgtgatt tcatcgtaat tcgtttcatc
    19021 tacggcactc gtagcctttt taatcgcccg ttccacattg tcgttaggca tatttgcagc
    19081 ttttgctttg tccacaacaa gccgcaaagc tggattcata gatggatcag gaccgcctgc
    19141 tttagctgcc atataaattt cacgagaaag tttctgaaag actttccctc gttttgcatc
    19201 ttgggcattt tttcttcctt ggatattatg ccatttacta tgtcctgaca tgatttttcc
    19261 tcccttgttc ttttgagaat acacttctat tctagtacta tttcacaaaa aaacaaagcc
    19321 acagccaatc tataaacgct tcttgaatgc cgcaaaaagc acacataata tcacagcagt
    19381 cagagcaccg atcgaatcca acatgacatc ttggaacata ggtgttcgtc caccggtcat
    19441 catctgatgg aattcgtcca aacctgcgta ccctgttgct gaaagccaag caaggatcgc
    19501 tgtcagccac caaattttta gcttggggaa aacacctaaa aacaagctgc ctcccaaaat
    19561 aaaataggta ccgaaatgtg cgcccttcct taaaaagaac tctaaaaatt tggcgtagcc
    19621 cagatggtcg atactcacca tacttccacc ataattaaaa cgtatacccg ctaatgtgtc
    19681 tttaaatggc tgattaggca gccagctttc taatcgcgaa acttgtgatt gctgtccgta
    19741 agtttgagaa gaactgtaaa ataagattat aacgactatc agtgcgatta ctaaataaat
    19801 atttccgttt ttcatttgtt tttttgaaga attcataagt cacctctgta ttttatctta
    19861 gacttttttt gaagaaacaa caatcatttt catagagact tcttccataa aaaagtgcta
    19921 tacttaattt tgtattacaa tatgaaggga gttttttcat gagttattca ttaaattgga
    19981 atctagattc tattttttcc ggaggcagtc attctgatgc tttgaaccaa cggatgaagc
    20041 aattggaaga ccaaatgaac gagtattatc accgcgtaac aaaatggtca cctagttctg
    20101 acaatgcaga gcagttaaac gctattcttc aattgcaaga aacgattacg aatggtttta
    20161 cccaatgcag cagctatatt actgcattgc tttctgccaa tgtcaatgat tcagatgcaa
    20221 aggtcttatc aggcaagctt tacgcaatgc tgcctcgtct gcaatcagcc gaaactgtat
    20281 tgtcaaagaa atttgcagaa atatctgaca atgattggaa tcagatgctt tctcaatctt
    20341 cgtttgaaac aattgctttt cgtttaaacg agattcgtcg tgatggctca caattattat
    20401 cagaagctga agaaaacatt ataaatacgc tttctttaga tggtctaaat gcttggagca
    20461 gtcattatga tacgattgta gccagcattt cgattccctt cgaacaagat ggacaagtag
    20521 tcgaattatc tgctggacaa gccttcaata agatgatggg cgatccagat ccaaaagtca
    20581 gagaaacact tttcgcagca tgggaagaag cctgggaaga aaaggcaccc ctcttcacgg
    20641 atactttgaa tcatttagat ggttttagac tttctgacta tgaacttcat ggtgtcacag
    20701 attttctaca aaaaccatta gagtacaatc ggttgaagaa agaaacattg tctgtcatgt
    20761 gggatacaat ccagaaaaac aaacaacccc ttgttaatta cttgactaga aaagctaatc
    20821 ttttcggtaa agaaaaaatg gaatggcaag atcaagatgc acctattatt ttaggcgatt
    20881 tgaaagaaaa aacctttagt tttgacgaag cagcagcatt tattatcgaa aactttaaca
    20941 aattcagtcc aaaaatggct aaatttgccc aatctgcttt tgaaaagagc tggatcgaag
    21001 cagaagatcg tcctgggaaa cgtcctggtg gttattgtac tgagttacca gaaacaaaag
    21061 aatcaaggat ctttatgacc tacagtaatt ctgttaatga ggttgcgaca cttgctcatg
    21121 aactaggaca tgctttccat agcagcacga tgtgggattt accttcttta aatcgcgaat
    21181 atgccatgaa tgtcgcagaa acagcaagta cattcgcaga actgatcgta gccgatgcta
    21241 cattaaaagt cgcaaaaaca aaagaagaaa aaatcaattt gctagataca aaactgcaaa
    21301 atgctttagc tatgttcatg aatatccatt cccgattcat ttttgaaaat cgattctatg
    21361 aagcacggca agaaggatta gtctcagaag ataagatcac agagatgatg gttgaggcgc
    21421 aaaaagaagg ctatcaagga gctttagcta cttaccatcc atatttctgg gctgcaaaat
    21481 tgcatttctt tattgatgac gtaccgtttt ataacttccc atacacattt ggctacctct
    21541 ttagtttggg catctatgct tacgccaacc aaaagggagc aaattttgaa gaagaatata
    21601 tccatttact acgcgatact gcctcaatga cgacggaaga tcttgcgaag aaacatttga
    21661 atgtcgatct tacccaacca gatttttggc aagcaggtat cgacatggtg actgaagata
    21721 tccaaacgtt catggaattg acagaagaat ttatctgatt tctattttat cacaataaaa
    21781 a
//
LOCUS       DACKAC010000046        21072 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.046, whole genome shotgun sequence.
ACCESSION   DACKAC010000046 DACKAC010000000
VERSION     DACKAC010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.046"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..178
                     /locus_tag="I0B46_001817"
     CDS             <1..178
                     /locus_tag="I0B46_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAR2084113.1"
                     /translation="KTVETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRA
                     QRRFEGTLRYSLGY"
     gene            complement(313..828)
                     /locus_tag="I0B46_001818"
     CDS             complement(313..828)
                     /locus_tag="I0B46_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-keto-6-deoxy-D-glucose epimerase"
                     /protein_id="HAR2084114.1"
                     /translation="MVVVMVKVFDIIKFSDFRGYTAVALDNETLFSIGIEFHIKQINQ
                     GFSHNKYTMRGLHYQSGEHAQGKIVMCLHGEIMNIALDIRQGSSTYGCVYVNTLSESN
                     GKCAYIPRGYAHGYLTMEDNTLVQWCVDNDFNSNAAKCISYESVDIEWPVSRQCFVIS
                     EKDKNVERLSF"
     gene            complement(1010..1621)
                     /locus_tag="I0B46_001819"
     CDS             complement(1010..1621)
                     /locus_tag="I0B46_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAR2084115.1"
                     /translation="MIDIISAITVNVLTALYQPFLFAVLTAYLFMYWYLFIVDNSRDP
                     KNLKDSVLIWLRYFKASSKFRRLFFLAFYFTLILFKTLLNRNMWMNPLSNVIGQWSLY
                     IIDNATGESRLTTECVENTMLFIPFCMLLLWWRDIKCDIIRTIYVGIKYVFLFSLSIE
                     FTQLFFRLGTFQLSDLFYNTLGGLIGALLYWLFYRLNKYIDLK"
     gene            complement(1647..3098)
                     /locus_tag="I0B46_001820"
     CDS             complement(1647..3098)
                     /locus_tag="I0B46_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAR2084116.1"
                     /translation="MTSNNLIVKTLSRYKNTPLPVKAALWFMVCGFLQRGVSMITTPI
                     FTRVLSTEQYGLYSTYLSWESLLLMITSLSLFKAMMNLYVKYDNQEHILSTVSGLTLV
                     LTIFWFVIAIFIKDILAEILGLTPVLVVCMFSYFIFQAEIQCWSLHKRYIYDYKTLLV
                     ATLAVTVGSSVVGCLAVLFISPTAEARALMNVLVTGVVGVAVYISVFRRCKDFKDNEV
                     WNFAVVFCVSLMPHYLSEFILNGSDKIMINYLCGSSDVAIYSVAYSIGALINLVTNSV
                     NSTFAPYQYQKIKSGEYDLLSKRANEVLGLVAAMLFALMLFSKEIVLIFGGQKYIDSV
                     NLIVPICIGIYFNYMFQLFARVQEYFEKKLTVVIPSVLCAIMNIVLNYVFIKLYGYQA
                     VAYTTVFSYMFFCVIHYIFYRKVCKELLDGKNIYDVRGILLISAITIAGGILISIINK
                     FDFIKYLIIAIACVAAIIFRKRLLLLVRRIMSK"
     gene            complement(3122..3853)
                     /locus_tag="I0B46_001821"
     CDS             complement(3122..3853)
                     /locus_tag="I0B46_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084117.1"
                     /translation="MEKKMTVIITMAGLGSRFRKIGYAVPKYMINAKGRTLFDWAMDS
                     LVGYNKNVDKYIFVVRQEDNASAFIKEHCAYFGIENVEVIEIDYMTDGQATTCMLAIP
                     YCNQDNEIMVYNIDTYVESNEMKYEDICGDGHIPCFHADGDHWSFAKLNDRNEVIEVR
                     EKVRISDNCTLGAYYFSSAKLYKALYEEYYADSSNMEKNEKYIAPLYNFMIKKGLKVT
                     ISIVDAKKVHVLGTPEELNVFLNQE"
     gene            complement(3871..4266)
                     /locus_tag="I0B46_001822"
     CDS             complement(3871..4266)
                     /locus_tag="I0B46_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HAR2084118.1"
                     /translation="MYSEYSFVFDIDGTLCPIKKKDERYEDLVPYENMVEKLRFYKEN
                     GAKITLFTSRNVNSYKGNLGMINANTAKVLLAWLDKWDIPYDEILYGKPWPGHKGFYV
                     DDRTVRPDEFLRCSVDELNEICNSSKKRF"
     gene            complement(4368..6005)
                     /locus_tag="I0B46_001823"
     CDS             complement(4368..6005)
                     /locus_tag="I0B46_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HAR2084119.1"
                     /translation="MKVLIPAAKVVEKELQSIGMLPAIIYPINKKIVFDYLYNQYNAI
                     CDSLDIICYEKADKVHRRLKNYHDSRIHICDLPELGDLGQTVYFGLSDEDEPVLINFA
                     DTIVMESMSDIEMDTFYYSEDFVSSTWTYFKINEGIITDIHDKEEAYVSDKREKLFIG
                     VFMFSSSKLLKSCLREAVESRKQGGINSFYRALQLYSERCPLKAVRTEKWFDIGHIDK
                     YYNTKLEVKAREFNHISIDKDRGILKKTSDDKDKFIGEIKWYLKLPADIEYVRPRIFD
                     YSTSYVAPYVDMEYYAYHTVHELYLYGDLKYNQWHDLFSRIKFICSDFKRYTLKDEKI
                     RESLEDMYLRKTMQRFQKLSEDKRFSAFFEKQIQINGRKFISLTGVIELLKNLIPSML
                     YDVNEFCIIHGDLCFANMMVDSNCSFVKVIDPRGKFGKYDIYGDSRYELAKLFHSLDG
                     KYDYIIKDLFTVEYDTECAVIDYTINTRVRDYDLFELFKSVFSDEIKDDFDKIVLIEA
                     LLFLSMIPLHGESFDHQIVMLATGMELLARVSNKVIL"
     gene            complement(6040..7374)
                     /gene="gmhB"
                     /locus_tag="I0B46_001824"
     CDS             complement(6040..7374)
                     /gene="gmhB"
                     /locus_tag="I0B46_001824"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HAR2084120.1"
                     /translation="MKTVIMAGGRGSRISELFPDIPKPMIPIDGIPVLERELCSLRDQ
                     GFNDIILTVSYLHEKIEEHFGDGSKLGIKIKYFIEKTPLGNAGALYFLLDELGDEPFL
                     LLNADAVFDVDFRQMIRFHVENNADVTLFTHPNSHPYDSGLIISDDNKKVLQWLAKED
                     QRPEYYKNRVNAGLHVINPKVLRENPLMEIVNKSIVEGGGLPYKIDLDRMILKPLANT
                     GRMYCYDSPEYVKDMGTPDRYRAVIKDFQKGIIVGKNLSNPQKAIFLDRDGTINKYVG
                     FLRNAEEFELIDGVSEAIKIINSLGFLCIVITNQPVIARGEVTVDELNSIHNKLETML
                     GHAGGFLNGIYYCPHHPHKGYAGEVIELKIDCNCRKPKPGLLLEASKDFNINLSQSFM
                     VGDSESDVIAGKNAGCKTVLITEDITLPANYGQDYSVCSLLEFVKNHLCTMQ"
     gene            complement(7416..8525)
                     /locus_tag="I0B46_001825"
     CDS             complement(7416..8525)
                     /locus_tag="I0B46_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084121.1"
                     /translation="MNKKEILYNILKKYENLPYVFRNMFGHNISKLVSWKLTADCATV
                     EENSEVYAAFQLRSKDMSDVPVMGLVNCCKDVAIVMQGPLCLKDDFSYNTLAYYKKCY
                     PNALIILSTWKDEDIKALKRIEDLGVIVICSEKPEKPGHLNINYQVGNTLPGIVRAKQ
                     LGAKYVCKTRTDQRIYHPNAMAFFCSLLEQYPVNNEDDWKLIQNQRLLLLSMPYGDMF
                     FPYCLSDFLYFGDVDDMINLFSIPSDVREKGAVSRGVSRREISENNLAPEVQLLRSYI
                     SRMGGNEECSIRAYWEFVKNHVITINKNQIDLYWHKYVGRYSNNTIYGTYYIDDSQDA
                     LHCYNFDFINWLNLYTGKYEYKAEYEKYMDFVWEE"
     gene            complement(8550..9785)
                     /locus_tag="I0B46_001826"
     CDS             complement(8550..9785)
                     /locus_tag="I0B46_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084122.1"
                     /translation="MQISKWKIIQFLMVFLMIISSSILKPLGGWLGETMNVRLVTVIL
                     AIVCLLIASLPAFRKNRYLNFCTLLICVVIIAAYIYSDKTIWVKSRESMSLYYVLLAY
                     PLFCTLINQAWNLDSMLNTICGVTFLSYILRTSISLIQKFSGVLLYENIYRECAPENW
                     YRGGFLRINPPCFTIIIIPIALYMIERQVVARRKVKYYLLIASALAYSAVIHGSRSVL
                     VYQIIVLGCYILFKKGSSKRKIAMWVLAGLLVVIFINSTMFSTFVETFTNSTGEYAGS
                     AESRFASVWFFIPKFLQSPLFGTGILTGEEFTFILPGGVRGHLSDIGFFYTITQYGVG
                     ILIFDILFIIKGFKTALLASKVGMTGYQYLAFGITLNVLLTGINIDWFYPIFTPAIPV
                     CLAVIEYIYQECRSVANIE"
     gene            complement(9816..10946)
                     /locus_tag="I0B46_001827"
     CDS             complement(9816..10946)
                     /locus_tag="I0B46_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAR2084123.1"
                     /translation="MIGYKVSIMKVLMCESQMPENWYSFDLSKELNQYVDLTLLCKRD
                     VPPLDPKIKSKHILYHKSTNKVFSALVFLKSLFLIWKEIRFGDYDIVHLQLMKSLKYE
                     GGLYVLCKRHYKKLIMTVHNVLPHEVREKDRQEHFNIYSNCDGFIVHNRVTEQHLYAE
                     FPELNAKSYVCPHAIYGDVKHISRAYDKDKFEFILFGQIRPYKGIDVLLNAITELPMD
                     VRKKVHFTIAGQQHPKQDSTDYKKMIEDLKVSDCVLYKPERISDEEMDSMFRNADAAI
                     FPYKEIYGSGALLMAYTYCLPTIVSNVPAFMEETEEGKIGLLFENLNSKSLADTIVKY
                     VNLSPAEKSKIEDDIYELVNRKYTWAASAKLTYEAYKQILNE"
     gene            complement(10933..11706)
                     /locus_tag="I0B46_001828"
     CDS             complement(10933..11706)
                     /locus_tag="I0B46_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAR2084124.1"
                     /translation="MLYFVIPLMSAKAANNWEMVSTLFNRTLWSCYNQTDPDFKIIVA
                     CHEIPKLTHEYDDRVEFIQVSEKDAPIPTNQDEKMIDKGYKTHALAMRLRELGGGFAM
                     MVDADDLVSSHLAEYVNSHPQENGFYVKTGYVYFVGDDYMKVLPKFSSGSACIVKYAV
                     EDLPEAYPEVMTANCDDNPWIIRKRHGGVVPACEEQGRPLKPLPFKAAVYVLGTGDNH
                     SLYGKTTKYQTKMREILEMFIAKRKIKGKLKAEFSVDWL"
     gene            complement(11720..12175)
                     /locus_tag="I0B46_001829"
     CDS             complement(11720..12175)
                     /locus_tag="I0B46_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HAR2084125.1"
                     /translation="MNAYKLYKVERWFYLHKMEFFAKIVKALIYVLHNSSISYQTEIG
                     DNCKFLYGGIGCVIGKDTKIGNHVIIGTNVLTGGRSNKPGMPTIGNNVYIATGAKILG
                     NITIGDNVIVGANAVVINDVEPNCSVGGVPARVLKKDIDVNDYCSLNRY"
     gene            complement(12597..13703)
                     /locus_tag="I0B46_001830"
     CDS             complement(12597..13703)
                     /locus_tag="I0B46_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAR2084126.1"
                     /translation="MKILIVVTMTNNFGKKGSYNSQEIGLARGLAELGHEVIVYKSVK
                     SDEEVGTEQLSNTAMIHYIKCRYVGSNGFLSTKNFDKNADVLIQFADLQLSVPHVYSW
                     TVKNGIKYIPYNGITRSQSGNKYIKTIIEILAHRNYKIYKKNGCVAKNDRVSEKLVQL
                     GVNKVKLAPVCVDFEQLHKNYEAIDFTTLREKWNIPRNVRVILYVGRFEKDKRPLDMI
                     SLFNKLDEHNYLLMVGTGALLEQTKQQINLLGLQNRVKIIERIPNKDIWELYCLSDVF
                     VNLNYTEIWGMVLLEAMYYRLGIIAVNAPGPQNIILDNRYGMLAKSDDEILTFLKDGR
                     FTNDIIDAAYQRVTTEFRWSKSGEIMLSMLEGDK"
     gene            complement(13721..14482)
                     /locus_tag="I0B46_001831"
     CDS             complement(13721..14482)
                     /locus_tag="I0B46_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HAR2084127.1"
                     /translation="MEKNMYKRKRDFTVCGIVGVCATTKNEFVREVFSMPTSPVSATV
                     DLVGVPAIISAKENPKIAEMYSQATMAAIDGMPIVKIGRRKGFTCERCAAPDIMGMVF
                     EEGVRQGKTHYFYGGKDNEVLNKLRANLERDYPGIKIVGMYSPPFRPLNEEEDSKICE
                     EINNLNPDFLWVGIGAPKQEMWMWEHQKKIHNTVMFGVGAGFNFFAGTLEKAPEWMEK
                     ASLEWLFRLVKEPRRLWRRYILGGIKYIYYTIESK"
     gene            complement(14558..15556)
                     /locus_tag="I0B46_001832"
     CDS             complement(14558..15556)
                     /locus_tag="I0B46_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAR2084128.1"
                     /translation="MKKTIMVTGGCGMIGSNLVKRLVKEGCWDVYVADNLWRGKLEYL
                     NDEDGHPVIDLDTHFFNADLTDYEQAKKVIGTTEYVVHLADVVAGIDYVFKNQGELFR
                     INNLINSNVFDCCRKAGKEKIKGVIYVGTVCSFPLTRQNTLNPEPLREEELFPALPES
                     AYGWSKLMGQLEMGYLEKETGIPCCILMLHNVYGTPTDFGERSQVIPALIRKAINNPE
                     EPFNVWGSGEQGRAFIHVNDIVNALVLALDKGWGHGHIQIGPSHCTSIKEIAYKIIEI
                     SGKDIKPFFDTSKPEGDKARCADYSKAKEILDWEPTVSLEEGLRESYNWIEKQIKG"
     gene            complement(15575..16228)
                     /locus_tag="I0B46_001833"
     CDS             complement(15575..16228)
                     /locus_tag="I0B46_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAR2084129.1"
                     /translation="MLAEKLNKHIDLLIKRYPTLETIKQDIIDGYLIMEECYLNGGKL
                     LVAGNGGSAADSEHIAGELMKRFKTPRPVSKEFADKLVAIDPVRGPELAKNLECGLTA
                     IPLVAHEALSTAYINDVDGLGVFAQQLFGFGRSGDVFLGISTSGNSKNIMSATVVARA
                     LGIKVIGLTGAKGGELATVADVCVKAPEVETYMIQEYHLPIYHCWCLMLEDKFFGNE"
     gene            complement(16238..17248)
                     /locus_tag="I0B46_001834"
     CDS             complement(16238..17248)
                     /locus_tag="I0B46_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HAR2084130.1"
                     /translation="MIITKTPFRMSFFGGGTDMEDYFKENGGAVLSTTFDKYCYVNVR
                     HLPRFFDYSAELSYSRTERVTSIEDIEHPAIRNAMKMLDMHEIRLTYEADLPARSGLG
                     TSSSFAVGMLNAFYALEGKYADKKKLADEAIYLERVLCNEAGGWQDQIAASYGGFNRI
                     NFNVDGYEVLPVIISPERKKQLNNNLMMFFTGFTRFSSDVQKANNVSGTEDKRVRLKK
                     MYELVDEAEAILTDKNRNLDDFGRQLDVTWRLKKGTGGAISTGSIDELYEKGMAAGAL
                     GGKLLGAGGGGFLVFYVQPEKQDAVRWAMRDLMYIPFEFENGGTRVIHYTPETYIEKQ
                     NG"
     gene            complement(17267..18517)
                     /locus_tag="I0B46_001835"
     CDS             complement(17267..18517)
                     /locus_tag="I0B46_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HAR2084131.1"
                     /translation="MKKQYNIAVAGTGYVGLSIATLLAQHHKVTAVDIIPEKVELINN
                     RKSPIQDDYIEKYLAEKELNLTATLDAKAAYQSADFVVIAAPTNYNSRTQHFDTSAVE
                     AVIRLVMEVNPDAIMVIKSTIPVGYTASVRVKTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMDDERLVEDAHTFATLLQEGAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPENLIEAIVESNRTRKDFIADRVLQMAGYYNYAEANQYDAGKEKPVVIGVYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPTLKDGETFFGSKVVNDLEQFKAMCDSVI
                     ANRYDACLDDIKEKVYTRDIFQRD"
     gene            complement(19863..20036)
                     /locus_tag="I0B46_001836"
     CDS             complement(19863..20036)
                     /locus_tag="I0B46_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HAR2084132.1"
                     /translation="MNRYVHGTVQKKNRKINGKLLVNPNGYEWLRAKGSYPVPKYCNY
                     SETLMEWYQLIIT"
     gene            complement(20379..21068)
                     /locus_tag="I0B46_001837"
     CDS             complement(20379..21068)
                     /locus_tag="I0B46_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAR2084133.1"
                     /translation="MESEDLLSRISEVHPNQVWLDKEKVEKRYIYLFFKRMIDIVGSI
                     IGLILASPLMFYVAYRIRKEEPGSPIFFCHTRVGKNGKTFKMYKFRSMCLDAEEKLEE
                     LLDQNEIEGAMFKMKEDPRVTKIGKIIRAKSIDELPQLWNVLKGDMSLIGPRPPLERE
                     VAEYTRYDLQRLYVKPGCSGLWQVSDRNNVSFHKMVELDLEYISHRSLMYDLRLILKT
                     FYVIVKPNGAY"
BASE COUNT         6751 a         4543 c         2911 g         6867 t
ORIGIN      
        1 aaaaacagtt gagactgttt tttcttcctt agaaaaatta ggttgtcaaa atttcaactc
       61 acgttctgtt aaagggctag agagtagatt tgaaagcata ttactggctt acagtgttct
      121 attaagtcga gcacaacgac gttttgaagg aactttgaga tattctttag gatattaaaa
      181 ttaactagca ccacgggtat atctatatct aaatttagtt ttatgtattc ctcatagaaa
      241 taaccgagca tttgctcggt tattgttctt cttattttcc agtatccttc ttttctccac
      301 agctcatttt aatcaaaagc ttaatctttc cacatttttg tctttctcac ttataacgaa
      361 acactgccga ctgaccggcc attcaatatc tacgctttca taacttatac atttggcagc
      421 attagaattg aaatcattat ctacgcacca ctgaactagc gtattgtcct ccatagtaag
      481 atagccatga gcatatccac gtgggatata cgcacattta ccattgcttt cactcaatgt
      541 gttgacatac acacatccat aagtggaaga tccttgtcga atatcaagtg cgatattcat
      601 aatttcacca tgtaggcaca tcactatctt tccttgagca tgctctccac tctgataatg
      661 tagccctcgc atagtatatt tattatgact aaatccttga tttatttgct taatgtgaaa
      721 ctcaatacca atcgagaaaa gagtctcatt atccaatgca acagcagtat atcctctgaa
      781 atccgaaaac ttaattatat caaacacttt taccataact accaccatgt tatatatttt
      841 accaattttt taattcgctc gacctcattt aaagcaataa aattccctac ctcttgaaga
      901 tataacaaca atgttttagc aaaaatagat ggcatgtaaa gtattcttga tgagattaca
      961 caatcctgtc tgcagaaaaa tattaaccat attaaaattc aaagcagttc tattttaagt
     1021 ctatatactt atttaatcgg tagaatagcc aatatagcaa agcaccaatt aagcccccta
     1081 gggtgttata aaacaaatcc gatagttgaa atgtaccaag cctaaaaaac agttgtgtaa
     1141 attctatact taatgagaat aaaaacacat acttaatccc aacatagatg gtgcgtataa
     1201 tatcacattt aatgtcacgc caccataaca ataacatgca aaaagggata aacagcatcg
     1261 tgttctccac gcattctgtt gtcaatctgc tctctcctgt agcattatct ataatatata
     1321 atgaccactg tccaataacg tttgaaagcg ggttcatcca catatttcga ttaagcaagg
     1381 ttttaaataa tatcaaagta aaataaaacg caagaaaaaa gagtctgcga aatttactag
     1441 atgctttaaa atatctcaac cagattaata cagaatcttt taagttttta ggatctcgag
     1501 agttatcaac tataaataga taccagtaca taaacagata cgctgttaat acggcaaata
     1561 agaacggctg atataatgct gttaatacat ttaccgttat agcagaaata atatctatca
     1621 ttctctatct ctctatgagc aaatgtttat ttgctcataa ttctccttac taaaagtagc
     1681 aatcgttttc taaatataat tgcagcgaca caagcaattg ctataattaa atattttatg
     1741 aaatcaaatt tattaattat tgaaattagg atgccaccag ctatcgtgat tgcagaaatc
     1801 aaaagtattc ctctgacatc atatatgttt tttccatcca atagttcttt gcaaactttt
     1861 ctataaaaaa tataatggat gacgcagaaa aacatatatg agaatacagt tgtgtatgct
     1921 accgcctgat acccatataa ctttataaaa acataattta gtacaatatt cataattgca
     1981 cacaaaaccg atggaataac gacagtgagt ttcttttcaa agtattcctg tactctagca
     2041 aacagctgaa acatatagtt gaaataaata ccgatacata taggtacaat taaattaaca
     2101 ctgtcgatat atttctgccc accaaatatc aacacaattt ctttactaaa tagcattaat
     2161 gcaaaaagca tagccgctac caaaccaaga acttcattag ctctttttga tagtaaatca
     2221 tattctcctg acttgatttt ttgatactga taaggtgcaa aagtggaatt gacagaattt
     2281 gtaacaaggt taattagagc accaatagaa tatgccacgc tgtaaatagc aacatctgac
     2341 gaaccacata agtaatttat cataatttta tcagaaccat tcagaataaa ttcagataaa
     2401 tagtgtggca tcaacgatac acaaaatact actgcgaaat tccacacttc attatcctta
     2461 aaatccttac atcttctaaa taccgatata tacacagcca cgccaacaac accagtcact
     2521 aggacattca ttaaggctct cgcctcagcg gtaggagata taaacagaac cgctaaacag
     2581 ccaactacag atgagccaac agtaactgca agggtagcta ctaataaagt cttataatca
     2641 tatatatatc gtttgtgtaa agaccaacat tgtatttctg cctgaaatat aaaatatgag
     2701 aacatacaga caactagaac tggtgtcaat cctaaaattt ctgccaatat atccttaata
     2761 aaaatagcaa taacaaacca aaaaatagtc agtactaaag tcaatccgga tactgtcgaa
     2821 agaatatgtt cttgattatc atattttaca taaagattca tcatggcttt gaacaaacta
     2881 agcgatgtaa tcatgagcaa taaggattcc cacgatagat aagtgctgta taaaccgtat
     2941 tgttctgtag acaaaactct agtaaaaatt ggggttgtaa tcattgaaac ccctctctgt
     3001 aaaaaaccac ataccataaa ccataatgca gccttaacag gaagtggagt attcttatat
     3061 ctgcttaatg tttttacaat tagattatta ctggtcactg ctattttctc ctaaacacta
     3121 tttattcttg attcaaaaac acattgagct cttctggagt accaagcaca tgaacctttt
     3181 tcgcatctac tatgctaata gtcaccttta accctttttt tatcataaag ttatataatg
     3241 gtgctatata tttttcattc ttctccatat tagaggagtc tgcataatac tcttcatata
     3301 aagctttata cagctttgcc gatgagaaat aatacgcacc aagagtacaa ttatcagaaa
     3361 tgcgaacttt ttcgcggact tctattactt catttcgatc gttaagcttt gcaaaactcc
     3421 aatgatcgcc atcagcatga aagcatggaa tatgtccatc tccacaaata tcttcgtatt
     3481 tcatctcatt tgattccacg tatgtatcaa tattgtaaac cataatctca ttgtcttgat
     3541 tgcaataagg aatagccaac atacatgtag ttgcttgacc atctgtcata tagtctattt
     3601 caatcacttc tacgttttca ataccaaagt aagcacaatg ctcctttata aaagctgaag
     3661 cgttgtcttc ctgacgaaca acaaaaatat acttatcaac atttttattg tatccaacca
     3721 aactatccat ggcccaatca aataaagttc ttcctttggc attaatcata tatttaggaa
     3781 ccgcataacc aatttttcga aaacgacttc ccaaacctgc catcgtaatg ataactgtca
     3841 tctttttttc catagaacta acctccttat ttagaatctt ttttttgaac tgttgcagat
     3901 ttcatttaat tcatctacag agcatctcaa aaactcatca ggtctaaccg ttcgatcatc
     3961 tacatagaag cctttatgcc ccggccaagg cttaccatac aggatttcat catacggaat
     4021 gtcccattta tccaaccacg ccaagagcac ttttgcagta ttagcattta tcattcccaa
     4081 atttccttta taggaattaa cattgcgtga ggtgaacaat gtaattttag caccattctc
     4141 cttatagaat ctcagttttt caaccatatt ctcatacggc accaaatcct catatcgctc
     4201 atccttcttc tttatcggac acaaagtgcc atcgatatca aacacaaaag aatattcact
     4261 atacattttt ctcttccctt ctattgtatt aattaatata atcgtgcctt aatgcctaca
     4321 ttgctaaaac agtattgtaa ccgttattca ttaaggcaca atttatatta gagtataact
     4381 ttattactta ctcttgcaag aagttccata cctgtagcaa gcatcactat ttggtgatca
     4441 aaactttcac cgtgtaatgg aatcatacta agaaatagca acgcttctat caatacaatc
     4501 ttatcaaaat cgtccttaat ctcgtccgaa aagaccgact taaacaattc aaataaatcg
     4561 taatctctta cacgcgtatt aattgtataa tctatcactg cacactcggt atcatattct
     4621 acagtaaata gatccttgat gatataatca tatttaccat ctaaactatg aaatagtttt
     4681 gctaattcat atctgctgtc cccataaata tcatattttc caaattttcc cctaggatca
     4741 ataaccttaa caaaggaaca attggaatct accatcatat tagcaaaaca caaatctcca
     4801 tgtataatac aaaattcatt tacatcatag agcatacttg ggataagatt ctttagaagt
     4861 tcaattacac cggttaaaga aataaactta cgaccattaa tttgaatttg tttctcaaaa
     4921 aaagctgaaa atcttttatc ttcagacaat ttttgaaatc tctgcatggt ttttcgcaaa
     4981 tacatatctt ccaatgactc tctaatcttt tcatctttta aggtgtatcg cttaaaatcg
     5041 ctacagataa actttatacg gctaaacaga tcatgccact ggttatattt taaatcacca
     5101 tataagtata actcatgaac cgtatgatat gcataatatt ccatatcaac atagggagct
     5161 acgtaagatg tagaataatc aaaaattcta ggtcttacat attcaatatc agcaggaagt
     5221 tttaaatacc actttatttc tccaataaat ttatccttgt catcgctagt tttcttcaat
     5281 atccctcgat ctttatctat agagatatga ttaaactctc gtgctttaac ttccaacttt
     5341 gtattgtaat atttatcgat gtgtccaata tcaaaccact tttctgttct tacggccttt
     5401 agcggacaac gttcgctgta tagctgtaaa gcccgataaa atgagtttat tcccccctgt
     5461 ttccgactct caactgcctc tcttaagcaa ctcttcagaa gttttgacga agaaaacata
     5521 aatacgccaa taaataattt ttcacgttta tcggatacgt aagcttcctc tttatcgtgg
     5581 atatcagtaa ttattccttc attaatttta aagtatgtcc aagtactcga cacaaaatct
     5641 tcagaataat agaatgtatc catttctata tcagacatac tctccataac aattgtatct
     5701 gcgaagttaa ttagaacagg ctcatcttca tctgataacc caaaatacac ggtttgccct
     5761 agatccccta attcaggaag gtcacatata tgaattctgg aatcatgata gtttttcagt
     5821 cttctatgaa ccttatccgc tttttcataa cagataatat ccaatgaatc gcagattgca
     5881 ttatactgat tatacaaata atcaaagaca atttttttat taattggata gataattgct
     5941 ggtaacattc ctatactctg caattctttt tcaacaactt ttgctgctgg aattaatacc
     6001 ttcatctaat tactcctttt acaagtttca ctaaaccaat tattgcatag tgcataaatg
     6061 atttttaaca aattctaaca atgaacagac actatagtct tggccataat tagcaggcaa
     6121 ggtgatgtct tctgtaatca gtactgtctt acaaccagca ttttttccag caattacatc
     6181 actttcacta tctccaacca taaatgattg acttaggttt atgttaaaat cctttgatgc
     6241 ttctaataac agtccaggtt ttggcttgcg acaattgcaa tcaattttta attctataac
     6301 ctctcctgca tatcctttat ggggatggtg cggacagtaa taaataccgt ttaaaaatcc
     6361 accagcatgc cctagcattg tttcaagctt attgtgaata ctattaagct catcaacagt
     6421 aacttcacct cttgcaatta caggctgatt tgttataaca atacacagaa aacctagaga
     6481 attaataatt ttaattgcct ccgatactcc atcaattaac tcaaattctt ctgcatttct
     6541 caaaaaacca acgtatttat taatcgttcc atctctatct aaaaagattg ctttttgtgg
     6601 attggataga tttttaccaa caataattcc cttttgaaag tccttaatta cagctctata
     6661 tcggtccggt gttcccatat ctttaacata ctctggagaa tcgtagcaat acatacgccc
     6721 tgtgttagcc aatggtttca gaatcattct atctaaatca attttgtatg gtagcccccc
     6781 accttcaact attgatttat ttacaatttc cattaatgga ttttctctca acaccttcgg
     6841 atttataaca tgaagacctg catttactcg gtttttataa tattcaggtc tttgatcttc
     6901 cttcgctaac cattgaagca ctttcttatt gtcatcggat ataattaatc cactatcata
     6961 cggatgtgag ttaggatgcg taaacaaagt aacatctgcg ttattctcaa catgaaatct
     7021 aatcatctgc ctgaaatcaa catcaaatac tgcatcagca ttcaaaagta aaaacggttc
     7081 atcacccagt tcatccaaca aaaaatacaa tgcacctgca ttgccaagtg gagttttttc
     7141 aataaaatat ttgattttaa ttcctaattt tgaaccatct ccgaaatgct cctctatttt
     7201 ttcatgcaaa taggaaacag tcaaaataat atcattaaag ccttgatctc taagcgaaca
     7261 caattctcgt tctaaaactg gaatgccatc tataggaatc attggcttag gaatatccgg
     7321 aaacaattcc gaaattcggc ttcctcgtcc gcccgccata attactgttt tcataatatg
     7381 cccttcccta aagtactttt tactataaaa gttaattact cttcccaaac aaagtccata
     7441 tatttttcat attctgcttt gtattcgtat tttccagtgt acaaattcaa ccaatttata
     7501 aaatcaaaat tatagcagtg aagtgcatct tggctatcat caatataata cgtaccataa
     7561 atagtattat ttgaataacg acctacatat ttatgccagt acaaatcaat ttgatttttg
     7621 ttaatagtaa taacatggtt ttttacaaac tcccaatatg ccctaataga gcactcctca
     7681 ttgcccccca ttcgtgaaat atagcttcga agaagctgaa cttctggagc taaattattt
     7741 tcagatatct cccttcttga gactcctcta gacacagctc ccttttctct cacatcacta
     7801 ggaatcgaga agaggtttat catatcatca acatcaccaa aatacaggaa gtctgacaaa
     7861 caatacggga aaaacatgtc tccatacggc attgatagta acagtaatcg ctgattttgt
     7921 attaatttcc aatcatcttc gttattaaca ggatactgtt caagtaaact gcagaaaaac
     7981 gccattgcat ttggatggta tattctttga tctgttctag ttttacaaac atactttgca
     8041 ccaagctgct ttgctctgac tattccaggc aaagtgtttc caacttgata attaatattt
     8101 aagtgtccag gcttttcagg tttctcacta caaataacta taactccaag atcctctatc
     8161 cttttcaatg ccttaatatc ttcgtccttc catgtcgaaa gaataattaa agcatttgga
     8221 taacatttct tatagtaggc cagagtatta taagagaaat catcctttaa acatagtggc
     8281 ccctgcatta caattgctac atctttgcaa caattaacta aacccatcac tggaacatca
     8341 gacatgtcct ttgagcgcaa ttgaaacgct gcataaactt cagaattttc ttctactgtt
     8401 gcacaatcag cagtcaattt ccaagatacg agtttactta tattatgacc aaacatgttc
     8461 ctaaacacat atggtaaatt ttcgtacttc ttcaatatgt tatataaaat ctctttctta
     8521 ttcatgagat gcactcctat taatatttcc tattctatgt tagccacact tctacattct
     8581 tgatagatgt attctataac cgctaagcat acaggaatag ctggagtaaa tatcggataa
     8641 aaccagtcta tattaatccc tgttagcaag acatttaatg taattccaaa tgctaaatat
     8701 tgatagccag tcatacccac ttttgatgca agtaatgctg ttttgaaccc ttttataata
     8761 aacagaatat caaatataag tatacctaca ccatattgag taattgtata gaaaaatcca
     8821 atatcagata ggtgtcctct aactcctcct ggtaatataa acgtaaattc ctcaccagta
     8881 agaataccag taccgaacaa aggcgattgc aaaaatttag gaataaaaaa ccaaactgaa
     8941 gcaaatcgag attcagcaga tcctgcatat tcacctgtac tgtttgtgaa agtttcaaca
     9001 aatgttgaga acatggtcga attaataaat atgactacaa gcaacccagc taaaacccac
     9061 attgcaattt ttcttttcga agaacctttc ttaaaaagga tatagcaacc aagtacaata
     9121 atttgataga caaggacaga tctagaacca tgaataacag cagaatatgc cagtgcgcta
     9181 gcaatcagta aataatattt tacttttctt ctagctacaa cttgtcgctc aatcatatac
     9241 aaagcaatcg gtattattat aattgtaaaa catggtggat taattcttaa aaagcctcca
     9301 cgataccaat tttccggggc acactcacga tatatgtttt catagagaag tactcctgaa
     9361 aatttctgaa taagtgaaat agatgttcta agaatgtatg aaagaaatgt tacaccacat
     9421 atcgtattta gcattgaatc caaattccac gcttggttaa ttaaagtaca gaacaatgga
     9481 tacgccaata atacatagta taaactcata ctttccctag atttaaccca tattgtctta
     9541 tctgagtaga tgtaagctgc tattataaca acacaaatca gtaaagtgca gaaatttaaa
     9601 tatctatttt ttctaaatgc tggtaatgag gctattaaca aacatactat tgctagaatg
     9661 actgtcacaa gtctaacgtt catcgtttct cccaaccagc cgcccaaagg cttcaatatg
     9721 cttgatgaga taatcattag aaagaccatt agaaactgta tgattttcca tttactaatc
     9781 tgcatgttaa ttattattcc tttctaattg ttttactact cattcaaaat ttgtttgtat
     9841 gcctcgtatg tgagttttgc agatgccgcc caggtatact tgcggtttac aagctcatat
     9901 atatcatcct ctattttgct tttctcagct gggctaaggt tcacatactt tacaattgtg
     9961 tctgctaggc tcttagaatt caaattttcg aaaagtaacc ctatctttcc ttcctcggtt
    10021 tcttccataa aagcaggtac attactaaca atagttggta agcaataagt gtatgccata
    10081 agaagcgctc cacttccata gatttcttta tatggaaata ttgcggcatc tgcatttctg
    10141 aacatactgt ccatttcttc atcagaaatt ctctctggct tatatagaac acaatctgaa
    10201 acctttaaat cttcaatcat ttttttatag tcagtactat cctgtttagg atgttgttga
    10261 cctgctatgg taaaatgaac tttctttctt acatccattg gaagttctgt gattgcatta
    10321 agcagtacat caattccctt atatggacga atttgtccaa acaagataaa ttcaaactta
    10381 tctttatcat acgctctaga tatatgttta acatctccat agatagcatg tggacaaaca
    10441 tatgactttg catttaattc tgggaattca gcatataaat gctgttctgt tactcgattg
    10501 tgaacaatga aaccatcaca atttgaatat atattaaagt gctcttggcg atccttttca
    10561 cgaacctcat gtggtaacac attatgaact gtcataatta attttttata gtgtctctta
    10621 cataaaacgt acaatccgcc ttcatatttg agacttttca tcaattgcaa atgaacaata
    10681 tcataatcac caaatcttat ttccttccaa attaagaaca agcttttcag aaaaacaagt
    10741 gccgaaaaca ctttatttgt acttttatga tacaaaatat gtttgctctt aattttagga
    10801 tctaaaggag gaacatcacg cttacacagc aatgttaaat caacatattg attaagttct
    10861 ttagaaagat caaacgaata ccaattctct ggcatttggc tttcacacat caataccttc
    10921 ataatactca ccttataacc aatcaacaga aaattctgct ttcagttttc ctttaatttt
    10981 acgttttgct ataaacattt ccagtatctc tcgcattttc gtctgatatt ttgtcgtttt
    11041 accataaagc gagtgattgt cccctgttcc taatacatat actgctgctt tgaatggaag
    11101 aggttttaat ggacgccctt gctcttcaca tgctggaaca acgcccccat gtcgttttct
    11161 aatgatccat ggattatcat cacaatttgc tgtcataact tccggatatg cctcaggcag
    11221 gtcttccact gcatatttta ctatacacgc acttcctgat gagaactttg gtagaacctt
    11281 catataatcg tctccaacaa agtacacgta tcccgttttg acataaaatc cattttcctg
    11341 gggatgggaa ttcacatatt ccgccaaatg agaacttaca agatcatctg cgtcaaccat
    11401 catagcgaaa ccgccgccca actctcgcaa acgcattgcg agagcatgtg ttttataacc
    11461 cttatcaatc attttttcgt cttgatttgt gggaataggg gcatcctttt cgctaacttg
    11521 aataaattca actctgtcat catattcatg cgtgagttta ggaatttcat ggcaagcaac
    11581 aatgattttg aaatcaggat cagtctgatt ataacacgac cataatgttc ggttaaataa
    11641 ggtactaacc atctcccaat tgtttgctgc cttcgcactc attaaaggaa taacaaaata
    11701 caacatacaa tctctccttt tagtaacgat tcaaactaca ataatcattt acatcaatat
    11761 cttttttcaa tactctagcc ggaacgcctc ctaccgaaca attaggttca acatcattaa
    11821 tgaccacagc attagcccct actattacat tgtcccctat tgtgatatta cctaaaattt
    11881 ttgcgccagt agcgatatat acattgtttc caattgtagg cataccaggc ttattggatc
    11941 taccaccagt cagtacattc gttccgatta taacgtgatt acctattttt gtatcttttc
    12001 caattacaca acctatgcca ccgtataaga acttacaatt atctccaatt tctgtttgat
    12061 atgaaattga gctattatga agtacataaa ttagtgcctt tacaatcttg gcaaaaaact
    12121 ccattttgtg taagtaaaac catcgttcta ctttgtataa tttatatgca ttcatgtttt
    12181 atctccccat ctttttagca ggtacccccc acacttcgta gggtggaata gacctcaata
    12241 ccactgtatt tgcagcaact acagcacctt ccccaacgca taattctgtg ttagatttac
    12301 aaagaatttt agcacccaca cataaaacag cgttcttttt tactcgaatg atgatacgtt
    12361 cattactatc tgctcaacca cacgtcacat tctgatagac tttaacacca tcttcaagga
    12421 ttgtgtcaga atgaatcaca gtgcctacag aattatgaac aaattcaaca gagttcccta
    12481 tctgtgcacc tttaccaata caacaaccaa gaattttggc tatttttctc gtgcaattat
    12541 caactatgtt taaagcatat cgattcctca atgccattaa tttcgttact tccatatcat
    12601 ttatcacctt ccaacatgct aagcataatt tctcccgact ttgaccatct aaattctgta
    12661 gttacacgct gatatgctgc atctatgata tcgttggtaa atcgtccgtc ttttaagaat
    12721 gtcaaaattt catcgtcaga tttagccaac attccatatc tattatccaa aataatattc
    12781 tgtgggccag gtgcattaac agcaataatc ccgaggcgat agtacattgc ctctagcaaa
    12841 accatacccc aaatttcagt atagtttaga tttacaaata catccgataa gcaatataac
    12901 tcccaaatat ctttgttagg aatacgctca attattttta ctctattctg cagtccaagt
    12961 aaatttatct gctgcttcgt ttgctcgagt aaagcaccag ttcctaccat cagcaaataa
    13021 ttatgctcat caagtttatt aaataaacta atcatatcta acggtctttt atccttttca
    13081 aaccgtccta catataaaat aacacgaaca ttgcgaggaa tattccattt ttctctgaga
    13141 gtagtaaaat ctattgcttc gtaattctta tgtaactgtt caaaatccac acaaacagga
    13201 gcaagtttca ctttatttac tcctaattgt actaattttt cagaaactcg atcatttttg
    13261 gcaacacacc catttttctt ataaatttta taatttcgat gcgccaatat ttcaataatt
    13321 gtctttatat atttattgcc actttgactt cgcgttatcc cattataagg tatatatttt
    13381 attccatttt ttacagtcca tgagtataca tgaggtactg atagttgaag gtccgcaaat
    13441 tgtattagaa catccgcatt cttatcaaaa ttctttgttg ataagaatcc atttgatcct
    13501 acataacgac acttgatata gtgaatcatc gccgtattgc ttaattgctc tgttccaact
    13561 tcctcatcac tttttacact tttatataca ataacttcgt ggcctaattc agctaaccct
    13621 cgagctaatc ctatttcctg agaattatag gaccccttct taccaaagtt attcgtcatt
    13681 gtaactacta taagtatttt catctatttt tattgccccc ctacttagat tcaatagtat
    13741 agtagatata ctttatacct cccaagatat accgcctcca tagtctacga ggttccttta
    13801 ccagtctaaa taaccactct agactagctt tttccatcca ttcaggagct ttttccaaag
    13861 tgcccgcaaa aaagttaaag ccggcgccta caccgaacat tactgtgtta tggatcttct
    13921 tctggtgttc ccacatccac atttcttgct taggcgcacc aataccaacc cacagaaaat
    13981 ctggatttaa gttgttgatt tcctcgcaaa tcttgctatc ctcttcttca tttaacggac
    14041 gaaatggtgg ggaatacatt ccaacaattt tgattcccgg gtaatctctt tctaggtttg
    14101 cacgaagctt atttaacact tcgttatctt ttccaccata gaaataatgt gtttttccct
    14161 gtctaacgcc ttcttcaaaa accattccca taatatctgg tgcagcacat cgttcacagg
    14221 taaatccttt gcgccgtcca attttaacaa taggcatacc atctattgca gccattgtag
    14281 cctggctata catttcagca attttgggat tttcctttgc cgaaataatg gccggcactc
    14341 ccacgagatc aacagtcgca cttaccggag atgttggcat ggaaaaaact tcccttacaa
    14401 actcgttctt tgtagtagca caaacaccta ctattccaca cacagtgaaa tcacgttttc
    14461 ttttatacat attcttttcc atctcagtac atccttactc atatgttctt cctccgcatc
    14521 ctctaattac acggaggaag aacagcttat atacttatta acccttaatc tgcttttcaa
    14581 tccaattgta ggattcacga agaccctctt ccaaagaaac agtaggctcc caatccaaaa
    14641 tctctttcgc cttagaataa tctgcgcaac gagccttatc accttcaggc ttagaagtat
    14701 caaagaaagg cttgatgtct ttgccagaaa tttcaatgat cttatatgca atttccttga
    14761 tggaagtaca gtgagaaggg ccaatctgaa tatgaccatg accccaaccc ttatccaggg
    14821 cgagcaccaa tgcatttacg atatcattga catgaatgaa agcacgtccc tgctctccgc
    14881 taccccacac attaaaaggc tcttcaggat tattgattgc tttacgaatc agagcaggaa
    14941 taacctgact acgttcgcca aaatcagtag gtgtgccata aacattatgg agcatcagga
    15001 tacagcacgg aattccggtt tctttttcca aataccccat ctccaactgg cccatcagtt
    15061 tgctccagcc ataagcagac tcaggaagag cagggaacaa ctcctcttca cggagaggct
    15121 caggattcaa agtattctgt ctggtcagag ggaaggagca tacggtacca acatagataa
    15181 cacccttgat tttctccttg ccagccttac ggcaacaatc aaaaacatta gaattgatca
    15241 gattattgat tcggaacaat tcgccctggt tcttaaacac atagtcaatg ccagccacaa
    15301 catcagccag atgaactaca tactcagtcg taccaataac ctttttagcc tgctcataat
    15361 cagtcagatc agcattgaag aaatgagtat ccaggtcgat gacaggatgc ccatcttcgt
    15421 cgttcaggta ctccaacttt cctctccaca gattatcagc tacataaaca tcccagcagc
    15481 cttccttcac cagtctctta acaagattgg agccaatcat accgcaacca ccggtaacca
    15541 taattgtctt tttcatttaa cattcctcca aattttattc gtttccaaag aatttatctt
    15601 ccagcatcaa acaccaacaa tgatatatcg gcaagtgata ctcttgaatc atgtatgttt
    15661 caacttcagg agccttaaca caaacatctg ctaccgtggc aagttcccca ccctttgcgc
    15721 ccgtcaagcc aataactttt ataccgagag ccctagcaac aacagttgcg ctcataatat
    15781 tcttgctatt tccggacgtc gaaataccaa ggaatacgtc accagatcta ccaaagccaa
    15841 acagctgctg tgcgaagaca cccaaaccat cgacatcatt aatataagca gtagacaatg
    15901 cttcgtgtgc gaccaaagga attgccgtta aaccacactc gagatttttg gctaattccg
    15961 gacctcttac agggtcaatt gcaactaact tatcggcaaa ttccttactt acaggacgtg
    16021 gcgtcttaaa ccgcttcatc aactcgccag caatatgttc cgagtcagcg gctgatcctc
    16081 cgtttcctgc cacaagaagt ttaccaccat tcaaatagca ttcctccata attaggtatc
    16141 catcgataat gtcctgttta atcgtctcaa gtgtaggata tcgtttaatc aataagtcaa
    16201 tatgtttatt aagtttttcc gctaacattg ttactcctta tccattctgt ttttcaatat
    16261 aagtttccgg cgtgtagtga ataactctag ttccaccatt ttcaaattca aacggaatat
    16321 acatcaaatc acgcatagcc caacgaactg catcctgttt ctcaggttgt acatagaaaa
    16381 ccaagaaacc tccaccacct gcaccgagca actttccgcc taaagcacct gcagccatac
    16441 ccttttcata tagttcatca atgcttcctg tactaattgc tccaccagta ccttttttca
    16501 atctccaagt aacatcaagc tgtctaccga aatcatctaa atttctgttc ttatcagtaa
    16561 gaattgcctc agcttcatct acaagctcat acatcttttt cagtcttact ctcttatcct
    16621 ctgtgccact gacgttgtta gccttttgaa catcagaaga aaatctagtg aatccggtaa
    16681 agaacatcat cagattgttg ttaagctgct ttttacgttc aggcgaaata atgacaggca
    16741 gcacctcata accatccaca ttgaaattaa ttcgattgaa cccaccataa gatgcggcaa
    16801 tctgatcctg ccatccaccg gcttcattac acagaactct ttcaagataa atggcttcat
    16861 cagcaagttt cttcttgtca gcatacttac cctccagtgc ataaaaagca ttcaacatac
    16921 caactgcaaa agaactggat gtacccagac cagagcgagc aggtaaatca gcttcgtatg
    16981 taagccgaat ttcatgcata tcgagcattt tcatcgcatt acgaatcgca ggatgctcaa
    17041 tatcttcaat gctagttact ctctcagtac gagaataaga taactcagcg ctgtaatcaa
    17101 aaaatctagg cagatgtctc acattcacat aacagtactt atcaaaagta gtagaaagaa
    17161 ctgcaccgcc attctctttg aaataatcct ccatatccgt tccaccgcca aaaaaagaca
    17221 tacgaaacgg tgtttttgta ataatcataa atactctctc ctaatcttag tctctctgga
    17281 agatatcacg ggtatatact ttttccttaa tgtcatcaag gcaagcatcg tagcgatttg
    17341 ctataacaga atcacacatt gccttaaact gttccaaatc attcacaacc ttactgccaa
    17401 agaaagtctc tccatctttc aaggtcggct cataaatcac aacagttgca cctttagctt
    17461 ttacacgctt cataacgccc tggatagaac tctggcggaa gttatcagaa ttggatttca
    17521 tggtcaaacg gtaaacacca atcacaacag gcttttcttt gccagcatca tactgatttg
    17581 cttctgcgta attgtaataa ccagccatct gaagtacacg atccgcaata aagtccttac
    17641 gagtacggtt agattcaacg attgcctcga tcagattttc aggaacatct gcgtagttgg
    17701 ccagaagctg cttggtatcc ttcggcaggc aatagccacc gtaaccaaag gaagggttgt
    17761 tgtaatgagt accaatacga ggatcaaggc aaactccatt aatgatctgc tgagtgttca
    17821 agcctttcat ctctgcataa gtgtccagct cattgaagta gctgacacga agagccagat
    17881 atgtattggc aaacagctta acagcttctg cttcagtaaa gcccatgaac aatgtgtcca
    17941 cattctcttt gattgcgcct tcctgtagca gtgtagcaaa agtgtgtgca tcctcaacca
    18001 aacgctcatc atccatatcg gtacccacaa taatacgcga aggatacaga ttgtcataca
    18061 gtgccttact ttcacgcagg aactcagggc tgaaaataat attcttgcta ccagtcttta
    18121 cacgaacaga agcggtgtat ccaacaggaa tggtggactt aataaccata atggcatcag
    18181 gatttacttc catgaccagc ctaataacag cctcaacagc ggatgtgtca aaatgttgag
    18241 tgcggctatt gtagttagta ggagccgcaa taacaacaaa atccgcagac tggtatgctg
    18301 cctttgcatc caatgttgcg gtcagattca gttctttctc agccaagtac ttttcaatat
    18361 aatcatcctg gattggagat ttacggttat taatcagttc aaccttctct ggaatgatat
    18421 ccaccgctgt aaccttatgg tgctgtgcca acagagtggc aatactcaaa ccaacataac
    18481 cggtgccggc gactgcaatg ttatactgtt tcttcataat atttcctctt tccaccactg
    18541 ctgtggtcac tactttctta acaaaatgct gtttgtgaac agtaacctgc tatttatata
    18601 aaacagcccg aaagctgtgt tcattcatac ttaaaacagc cagctcctac gccggcaaat
    18661 tcaatttttc ataaatcccc tacccgttta gggttatatt gcaagtttga gtcattttta
    18721 ttgacctcgt ttggggttat aatacatttt cgcattgaaa tgtacctaca ttttccactc
    18781 aaccagtcac caaaaagttt ataaattcac ttattaccct ctattcttac tccaaaggta
    18841 tgtttaacat cgctttattg acctattttc accccaaatg gaagtttgtt tggtacatta
    18901 ttggtctatt cccaccccaa actgtctatt ttgagcgagt ttttgttttt tatcattgca
    18961 cattttcaag gctcaaattc taagcggatt tactggcagg gagacattgg taaatacttg
    19021 aacaatttca cggctttcat catcacatcc tacgataata attctaattg gataaagatt
    19081 atcattaata atctactttc acgtaaaaat tctgaactaa ataaaatcta ttgataatat
    19141 ttttttcttc cactttttgt atatcctaca ggtatagtgg atttaattat ccccaatgcc
    19201 tctgaagtaa cagacatcac taattcaata acacattcac taccatagtt tgtaggaaca
    19261 aaaatgacaa caaaatctgc ttccttatgt gatgtttttc catctgtcca aattttatta
    19321 ccaatgaaat ctagtttaaa atccctctta gaaactagtt cactctatat ttttattcag
    19381 tttcattagt tctgtttttt tgtcttcagt caccatatac atatatatct taattatcta
    19441 tccatttcat cctacgcatt cttgcgtaaa tacaattcac tctgttgagc tgatataaat
    19501 cattggtggt acaaagataa aatgataact gtttctatac ctccaaaaat tccagtaaat
    19561 atatctatag atagtttcaa aaaaaattgt cttagtttca taatatttaa gatagtggta
    19621 catactttat aacaacgtac ttttccaaat attattttcc gttatgaaca tccaatatat
    19681 tacaaagttt aaaagtttag atgaactata atcttcattt ctgcacagtg gataataaat
    19741 tcttatagat aaaaaatagc ctcttctaaa gtcacaaata ccttactccc acattttaag
    19801 taaacactct gcagcattct ctttgttata aaatgataga tattcctcaa ttttatctct
    19861 tattatgtta ttatcagttg ataccattcc attaatgttt cagaataatt acaatatttt
    19921 gggaccggat aactcccttt tgctcgtaac cactcatatc cgttaggatt cactaaaagt
    19981 ttaccgttta tttttcgatt ctttttttga accgtcccat gaacataccg attcaacaag
    20041 ctaaattata aaaattggtg tgctgtcatt attttttgga aaatttatta atggctatca
    20101 atatactttt gtatcatact aaatagctcg tcagttccta tatcggatat accaatatta
    20161 aagcatttag atcattatat ttaagagtgg tatcatccca aaatttcgaa tattttcttt
    20221 tatataagaa taatgatact gaattgcccc attcaccttt ctttttttag attttctaca
    20281 agtgattcaa accctccata attacagtta ttacttttga aacgataata tgaacatgtg
    20341 taatcttttt tattaacaat ccatcattct atccctaact aatatgctcc attgggcttg
    20401 acaataacat aaaacgtttt taaaatcaac ctcaagtcgt acattaaact tcgatgacta
    20461 atatactcta aatccaactc aaccatttta tggaaactaa cattatttct atcactaact
    20521 tgccacaaac ctgaacaacc aggttttaca tacaagcgtt gaagatcata tctcgtgtac
    20581 tcagctactt ccctctccaa aggtggccgt ggaccaatta gactcatatc gccttttaaa
    20641 acattccata gctgtggcaa ttcatcaatg cttttggcac ggataatttt acctatttta
    20701 gtgacacggg gatcttcttt catcttgaac attgcacctt ctatttcatt ttgatcaagt
    20761 aattcttcca atttttcttc agcatctaaa cacatcgaac ggaatttata cattttgaat
    20821 gtttttccat ttttaccaac acgtgtatgg caaaaaaata tcggacttcc tggctcttct
    20881 tttctgattc gataagctac atagaacatc aaaggagaag ctaaaatcaa accaataatg
    20941 cttcctacaa tatcaatcat tcttttaaaa aataaataga tatatcgttt ttcaactttt
    21001 tctttatcca accaaacttg attaggatgt acttcactaa tacgagataa taaatcttca
    21061 ctttccatac tt
//
LOCUS       DACKAC010000047        20663 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.047, whole genome shotgun sequence.
ACCESSION   DACKAC010000047 DACKAC010000000
VERSION     DACKAC010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.047"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            168..362
                     /locus_tag="I0B46_001838"
     CDS             168..362
                     /locus_tag="I0B46_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084134.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..930
                     /locus_tag="I0B46_001839"
     CDS             631..930
                     /locus_tag="I0B46_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084135.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1027..1461)
                     /locus_tag="I0B46_001840"
     CDS             complement(1027..1461)
                     /locus_tag="I0B46_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAR2084136.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1693..2403)
                     /locus_tag="I0B46_001841"
     CDS             complement(1693..2403)
                     /locus_tag="I0B46_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAR2084137.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2393..3253)
                     /locus_tag="I0B46_001842"
     CDS             complement(2393..3253)
                     /locus_tag="I0B46_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAR2084138.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3258..3896)
                     /locus_tag="I0B46_001843"
     CDS             complement(3258..3896)
                     /locus_tag="I0B46_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAR2084139.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3911..4210)
                     /locus_tag="I0B46_001844"
     CDS             complement(3911..4210)
                     /locus_tag="I0B46_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAR2084140.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4253..5737)
                     /locus_tag="I0B46_001845"
     CDS             complement(4253..5737)
                     /locus_tag="I0B46_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAR2084141.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5757..6218)
                     /locus_tag="I0B46_001846"
     CDS             complement(5757..6218)
                     /locus_tag="I0B46_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAR2084142.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="I0B46_001847"
     CDS             complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="I0B46_001847"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAR2084143.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7521..8291
                     /locus_tag="I0B46_001848"
     CDS             7521..8291
                     /locus_tag="I0B46_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAR2084144.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8564..9391)
                     /locus_tag="I0B46_001849"
     CDS             complement(8564..9391)
                     /locus_tag="I0B46_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAR2084145.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9959..11194
                     /locus_tag="I0B46_001850"
     CDS             9959..11194
                     /locus_tag="I0B46_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAR2084146.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12193..12453)
                     /locus_tag="I0B46_001851"
     CDS             complement(12193..12453)
                     /locus_tag="I0B46_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAR2084147.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12480..13781)
                     /locus_tag="I0B46_001852"
     CDS             complement(12480..13781)
                     /locus_tag="I0B46_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAR2084148.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14073..14531)
                     /locus_tag="I0B46_001853"
     CDS             complement(14073..14531)
                     /locus_tag="I0B46_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAR2084149.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14544..15869)
                     /locus_tag="I0B46_001854"
     CDS             complement(14544..15869)
                     /locus_tag="I0B46_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAR2084150.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPIILRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15886..17298)
                     /locus_tag="I0B46_001855"
     CDS             complement(15886..17298)
                     /locus_tag="I0B46_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAR2084151.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(17315..18079)
                     /locus_tag="I0B46_001856"
     CDS             complement(17315..18079)
                     /locus_tag="I0B46_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAR2084152.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(18183..19058)
                     /locus_tag="I0B46_001857"
     CDS             complement(18183..19058)
                     /locus_tag="I0B46_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAR2084153.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(19312..19396)
                     /locus_tag="I0B46_001858"
     tRNA            complement(19312..19396)
                     /locus_tag="I0B46_001858"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19360..19362),aa:Leu,seq:caa)
     gene            complement(19414..19485)
                     /locus_tag="I0B46_001859"
     tRNA            complement(19414..19485)
                     /locus_tag="I0B46_001859"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19451..19453),aa:Gln,seq:ttg)
     gene            complement(19495..19567)
                     /locus_tag="I0B46_001860"
     tRNA            complement(19495..19567)
                     /locus_tag="I0B46_001860"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19532..19534),aa:His,seq:gtg)
     gene            complement(19629..19713)
                     /locus_tag="I0B46_001861"
     tRNA            complement(19629..19713)
                     /locus_tag="I0B46_001861"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19677..19679),aa:Leu,seq:caa)
     gene            complement(19734..19804)
                     /locus_tag="I0B46_001862"
     tRNA            complement(19734..19804)
                     /locus_tag="I0B46_001862"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19770..19772),aa:Cys,seq:gca)
     gene            complement(19850..19921)
                     /locus_tag="I0B46_001863"
     tRNA            complement(19850..19921)
                     /locus_tag="I0B46_001863"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19887..19889),aa:Gln,seq:ttg)
     gene            complement(19933..20005)
                     /locus_tag="I0B46_001864"
     tRNA            complement(19933..20005)
                     /locus_tag="I0B46_001864"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19970..19972),aa:His,seq:gtg)
     gene            complement(20008..20081)
                     /locus_tag="I0B46_001865"
     tRNA            complement(20008..20081)
                     /locus_tag="I0B46_001865"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20047..20049),aa:Trp,seq:cca)
     gene            complement(20088..20168)
                     /locus_tag="I0B46_001866"
     tRNA            complement(20088..20168)
                     /locus_tag="I0B46_001866"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20132..20134),aa:Tyr,seq:gta)
     gene            complement(20170..20245)
                     /locus_tag="I0B46_001867"
     tRNA            complement(20170..20245)
                     /locus_tag="I0B46_001867"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20210..20212),aa:Phe,seq:gaa)
     gene            complement(20281..20353)
                     /locus_tag="I0B46_001868"
     tRNA            complement(20281..20353)
                     /locus_tag="I0B46_001868"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20318..20320),aa:Asp,seq:gtc)
     gene            complement(20363..20435)
                     /locus_tag="I0B46_001869"
     tRNA            complement(20363..20435)
                     /locus_tag="I0B46_001869"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20400..20402),aa:Val,seq:tac)
     gene            complement(20451..20522)
                     /locus_tag="I0B46_001870"
     tRNA            complement(20451..20522)
                     /locus_tag="I0B46_001870"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20487..20489),aa:Glu,seq:ttc)
     gene            complement(20537..20626)
                     /locus_tag="I0B46_001871"
     tRNA            complement(20537..20626)
                     /locus_tag="I0B46_001871"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20590..20592),aa:Ser,seq:gga)
BASE COUNT         6531 a         4319 c         3274 g         6539 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa atcatgtgtt cattttttat attagttcga atttgcaaaa aattgtattt
      901 gataaagagg tgagtaaaaa aataaaatag ctgagcattg gaggatagat tgagtgatac
      961 taagtctatc ctcttgtttt tctaaagaac taagctagaa ttttttcttt tgtacgttat
     1021 tatatgttat ttgtcattac caaaaagcac cttttttctt ctagtatcta tgacataaga
     1081 aataccaccc atttccttta cttgaacaaa tcctactgat tcataaaatt tttttgtttt
     1141 ctcagtatca tctgtcaata attcgatttg gactggtcca taactagtga tcttctccaa
     1201 agcttcattc aaaagcactt tgcctattcc ttggcgctga tacctcggat gaatgaggat
     1261 atcttgaata aatgcgatcg tcgcaaaatc agtgatccaa cgaacgattc cgataatctt
     1321 cccttcccca ttacgcttta tcagagattc agattgttca aatgctctag ctaaccttgc
     1381 agtgtctttt gtgtaattcg tccaaccaac gctcttatat aattcagaaa tctcatcgat
     1441 cataagggaa tcaatattca tttttttctt cctttcttat acaattctcc ctctaataat
     1501 acagttcttt gactatttaa tctatcacat ataactctcc tgtaaagatt ctctcgattc
     1561 cttaaaattc ctctttcctt ttagaatgca tactttttga taaataaaaa ggttgtgata
     1621 aattttcttc acaacctctg tttctctcta tcaagtgtta tcttttaatt ttctaccctt
     1681 accctagcat ctctattgcc cgtagtatgc atactttcca tgtttgcgaa aataatgttt
     1741 atctagtaaa tattgcggga tttcacatat attggggttg atcagctcgt tttctttggc
     1801 catcagacaa atttcatcta agatcaaact gttttctact gctttcatag gtgaatcccc
     1861 ccatgtgaac ggtccatgtc catagacaag tactccaggg acttcgtttg gatctaattt
     1921 tctttcctta aacgtctcga caatcacatt ccctgtatga acttcgtaag cttctcttac
     1981 ttcttctttc gttaattggc gtgtacaagg aaccttacca taaaaagcat cagcatgagt
     2041 cgtgccataa gctggcaaat cgcgtcctgc ttgcgcccac atgacggagt gcgtggaatg
     2101 agtatgagta atagcagtta tatcttcaaa agtttgatac aaaacaacat gtgtggggag
     2161 atctgaagat ggttttaagc tatcttcttc tattatattt cccgacaaat ctgttaccac
     2221 catttgatct gcttgcatgc attcatatct gacaccgctt ggcttaatga cgatgactcc
     2281 tagctcacgg tccacttcac tcacattgcc ccaagtcaat ttgactaatc cgtatcgcgg
     2341 caatgccaga tttgcttcaa ggacacgttg cttcattgct gcaatttttt cttcacaaga
     2401 catcatagcc agcctccttc aatttagaac ttaagtagtc ttttgcctgc tggatttctc
     2461 ttcgaaagtc ttggcttttt tcactccaca tttcaatcag aaatgttcca tcataatcta
     2521 gacgtttcaa atgtcttaat aatccaagaa aatcaacaca accgctacca aaaggaacat
     2581 cacgaaactg gcctgaatat tcttttgtta cagggtaagt atcttttaag tggatagcag
     2641 tgatataatc gatccctttc tcaagttctc gagccggatt attttccggc catgcggaca
     2701 aattgcctag gtctggatat acttgtagaa aaggtgaatg aatctgttct ttgatttcta
     2761 aaaattttga aatggagttc ataaatggat catccatgat ttccacggaa agaatgatgc
     2821 catatttaga agcttccttt acgctttctt ttattccttc gataaagtct tctctggaat
     2881 tcattgattt ctcttcataa taaacatcgt atccagctat ttggattact ttgacgctta
     2941 gattatgcgc caaatggatt gcttttttca ttagttgttt cgctgctttt ttcttttctg
     3001 gatcagcaga accaaacgga aatcgccgat gcgcacttaa acaaatcgaa tggatacgta
     3061 caccggagct gaacatctct tctctcagct cagctatctt ttcttcagac caatctaatc
     3121 gtgctaaccg ctcatccgtc tcatcaatcg acatctcgat aaaatcgaag tccatttctt
     3181 ttacaaggga aaaacgttct ttccatgaaa tatctttggg taacgctttt tcataaatcc
     3241 caatttgagc catttcctta cccccagata cgaatgatct catctttgaa gttttgtgcg
     3301 gccttcatcg gatcatttgc tgctgtaata ccacgaccag tgataaaggt atacacatcg
     3361 atcccttcaa ataagtgtaa tgtgtgtgga tttaatccac cagttacaga aacattaaag
     3421 cctagttcaa tcagtttttt gatcttcgat aaatcttttt caccccaagt ttcaccagat
     3481 aacagcgcat cacggctttg atggtagatt acctgccgga taccgatgtt atgccaatcc
     3541 atggcttgtt caaaagtcca attgccgtag agttctactt gcaattcacc tatttctttt
     3601 tgtgctgcct ccatagttgg aatcgttgca caacaaatga ctgtcatcca gtctgctcct
     3661 gcttttgcaa cattactcgc cactgttcct ccagcgtctg cacatttcgt atcagcgact
     3721 agttttttat ctgggaacat actgcgaata cagcgaatcg ctttttgtcc ttcctgtaag
     3781 caaagaattg tgcccacttc aataatatct actacatcac caactttcat aacatctcct
     3841 aatgcatgtt ctaagtcatt gtgatctaac gcaatttgca aattcggcct gctcatctta
     3901 gttccttcct ttacttgttt ttttggtacc actttgtttc agcaaatttt tcactgattt
     3961 ctttgactga catcacattt ttgataccga taactgtaac tccttttttc tctgcctgag
     4021 taaacatatt caaaaaattg actggcgtaa aaactacatc gtattggcca gctgaacttt
     4081 ttccctctga aatggcacaa tgatggattt tggtaattgg tacgtttagt tctttcattg
     4141 ctttttccac acttctcatc atcatcaagc ttgttcctga tccatttgca cacgatacta
     4201 aaattctcat cttaatccct ccattatgag tgttgtctaa tcagattttc ttttatactc
     4261 tctgcttttc tgcatatgtg tcatagtctt ctacaatcaa aaaataacca tcaggatttt
     4321 tccgatattg taactgcgga atagccaata aaccaatgac aactacggca attccagcat
     4381 aacctaggac tttcatgatt actgtaaaca atggccaagc tgttgcccag tcgaacatcc
     4441 cgatataacc accgtatgtt gacatcccaa tccatgtagc gaataatgct gaaccgccta
     4501 cttgtatgat tcctgaaaga aacgggaaca ggcaagcggc tttgaatcca cctcggttat
     4561 tggcataaac tgctataacg gcattatcaa agaacaacgg aatgaaaccg gctatgacga
     4621 tcgttggtga tttcaataaa atcagtaaac caatcatcaa aaactgtccc aaagcaccaa
     4681 acaagaaccc aatcgttacc gcatttggcg aaccaaagcc aaaagtagca gctacgtcaa
     4741 ttcctggaac agctcctggt aaaatcgtgt ttgagattcc ttggaaagat tgagttagtt
     4801 catcgacgaa tgtccgaaca cctaattgca agatcgccag atataccgca aaatttaaag
     4861 aagtcgtcat aatgtagaat aagaaactct gcccttcttg catgaattgc gcttgaatca
     4921 aataatcttt gcctagtaca atcagaatga tcccaaagaa aaatagcatg agtaacgaag
     4981 tagcaaccat attctcattg aaaatcgaca agaatcctgg taatttgata tcttcaattt
     5041 ttttgtcttg cttgattgat ctatttttct tttcatcccg ctttttcatc cactcagcta
     5101 attttgcaaa tataaagatt ccgaacattt gctgatgtgc cactgcaaat ccggcacctt
     5161 ctgtcaattc ctgcgtgatt cctactgtta agttagaagc tactgtccaa taacaaccta
     5221 aaatcagtcc cataaaaatc aatgcttgta tcctacctag atcaggaaag caaaacagta
     5281 aaatccaaaa agcggtagct gcttgttgaa cctgaacgtt tccggtagtg aaaacggaac
     5341 gcagtttcgt atatttctga aaacggacta ataagatatt taaaacaaat gcaatcagta
     5401 ataaaatcat cacatctcca aaggtacggc caaatgtctc cattaatcca gcatccacag
     5461 cattttgtcc aaaataagga tctgtaacca tggcatccaa attgaaccgt tcctttagtc
     5521 caaccaagat tggccgaaaa ttattgacca aaccaccaga gccaacagtc aaaataaagt
     5581 aaccgaccgt tgcctttaag aatccagcga tgcattcatg aagcgggcgt ttcagcaaaa
     5641 tatagcctaa taacacgata aaaccaatca ggtatgctgg ctgcgtcaag atatttgttg
     5701 caaaatacgt ccaaatgctt aacaaaaatt cccccatctt ttttctcctc tatcaattaa
     5761 ttatattctg ccatcacaac ttggtaatca tcgatggagt tcgtagagat gagacgctca
     5821 accaatccat ctgtcattaa taattcggat aatttttgga tattttctag atgttcttct
     5881 ggatcttttg ctgcgagtgt aaaaaacaaa ctagccgttt tctcctcatt ctccccttca
     5941 ctttcaaaac ggattggctg tttcaacttg gtaaatgaaa tagctgttcc aaaaacacca
     6001 tcactttttt ctgaagaatg cggcattgct acttgaggtg cgatcacaat ataaggaccg
     6061 tatttttcta cacaatggat gatctcctcc acatacgttt catcaataat ttccttatcg
     6121 atcaatacaa gacagctttc ggcgatagct tcacgccaat ccgtgatttc tttatctggg
     6181 taatgaatca attgatggtc aaaaaagtat ttcagcaaga aactcccctt ctttcttata
     6241 aaaacgacgg aaacggtgta tcattttcaa tcgagaagac atcttcaaat cctcgataaa
     6301 aattaaactc aagatcatct ttgtttgtcg gatagatgaa tttcccgcct acttgccaga
     6361 tataaggttt aaatccatac ttcaggcgat cctttttgaa tttccaaatt tcagtgatct
     6421 cttttggatc agccataaaa ttagaccaaa tatcgtagtg gacaggaatc actacttttg
     6481 cattcaatga ctccgccatg cgcagcatat caacagaagt aactttgtct gttatccctc
     6541 gtgggttttc accataggcc cctaaacaga catcgatttt gtgttcatta ccatgtttgg
     6601 caaataaatt ggagtaatgt gaatctcctg catgataaag attccctcct gatgtttcga
     6661 ataaatagtt tactgcaatt tgatccatct cctgaggcat ttttcctttt aatacctctt
     6721 ctggatcttc acaagtgacc aacgctgtcc gatcaaacgc ttctaaagct actacattga
     6781 tgtctttgat tttgactgca tcacctggct ttactgtaat cgttttttct tcaggaattc
     6841 cccatttttg ccacgtgcga accacttctt ttggcccaat gaatgttgct tcaggacaat
     6901 tttgatgaac tgctgcggct gtatggatat ctagatgatc ggaatggata tgtgtaacga
     6961 ttaaagcgtc tacatttttt atcgcaaaag gatcgattac gaaaggctga gtacgtaaat
     7021 taggctgcat attttgaacg ccactcatac gcatcatctg atggcctttt ttcatcatgc
     7081 cattcccatg actacgtttt cccgtcccac accatagatc acataaaata ttcgttcctt
     7141 catgactttt cagccaaatt cccgtacagc ccagccacca catagcaaaa gttccaggat
     7201 ccactacttc tgtttcgatt tcttcattca agtatgtgcc ccactcagga aatgtttcta
     7261 ctaaccactt ttcttttgtt acttcattga cagttttact catttgcttg ctccccccat
     7321 atcgttgttc caacttacaa gtaaatatta atgtgtatca gcatccatga aaaccctttt
     7381 atatgaccgt ttgtttttta tatgattttc tttcagtaat caatgattgt ttcagtataa
     7441 taaagacaga tatgaatgtt taaatgaaca actagtcaaa taaatccatg gatcaagatg
     7501 tccctcagga ggcaaaagtc atgaaaaact cacttgtcaa tattgaaaaa cgtcaaaaag
     7561 aaattttgga actgcttcag cagactgaac aactatcaac tttcgaatta gctgaatccc
     7621 ttgatgtatc tattagtacg atacgaagag atttaaattt attagaagaa aaaaacgaga
     7681 tcatacgcaa ttacggttat tgttcttata attacaaaaa ccagactgat tttgatcaat
     7741 ctggtcctga aaggattaaa caagcgattg ccagagaagc tagtaaatac attaacgatt
     7801 atgatacgct gtttatcaat tctagctcga ctgcgttgaa aacactggat tttcttcaat
     7861 ctaaccattt gacagtcatt acaaacaacg tgaaaatcaa ttactctccg cacaaagata
     7921 attgtagtta tattttaact ggtggagaga tgcgctttcc aaaagaagca ctagtgggcg
     7981 acatcgcatt aaatacgatt tcttcagtca atgcagacat ttgtatcatt ggctgtagcg
     8041 gagtcgatgt agtacatggg gtcacaacaa agatattaaa tgaatcaacg atcaacgaaa
     8101 tgatggtcaa caagacaatc aaatgtaaga ttttagtcgc tgatcatcga aaaatcggac
     8161 tgacatcaaa gttcaagatt gctgatattt cagtctttga ttatttgatc actgataaat
     8221 tttgttctga aaaagtatta gaggagatca gaaatacggg gataaaaacc attcaagtag
     8281 attcatttta agaaccagga cgtaaatatg taaataggat tatcaaaaaa atgaataaca
     8341 aatttatcga taaatgaaaa acacgctacg ctcaaattga taaaggaatc ccaagatgta
     8401 tccatccaat cgataccatc ataatctatt gcttctttta taaccgttat atgttgccta
     8461 aataggaatc aaacaatatc gaaaaaagga ggtatgacaa aagtcacacc tccttttttc
     8521 gaataaacgg tattcaaatc agaacactga tttcacaacc actttatttc aggtcgtttt
     8581 caatcacctt taccgcgtaa tcatttccgt tgaaacgcgc tatttttttt gcgtccatat
     8641 atctttctaa ataatcatct tctcctaatt gcttatggca agaagcaatt tcataaagca
     8701 agtattccaa tgcataagtc gatttatgtt tcttattata ctcaataccc ttctcacata
     8761 attggattgc tttatcataa tcctcgattt tacggtaata ggtggcgata tcataatata
     8821 caatgcagac catcagatta tcaaaatcag tctcactcaa tattttaagt gtacgctcca
     8881 cataaacttt cgcaaaatct aattctcctt ttgacaagta gatcattgcc acgtttgcag
     8941 ctgcttgtaa gtgatggata tctgtttcgg tcgtttgttc caacacttga ttaaaataaa
     9001 ataatgctgc atctggtttt ttcgcataaa gatgatcaag cagtccttga taaaataaaa
     9061 tttgatttaa aagatcagaa ttacttactt tttcctgatc gatcgtttgt aaaattttat
     9121 aagattcttg gtattttgat acagaattca atgattgaat ctcatttatt tttttcctga
     9181 ttttgtcttc ttctgtttct actaatatat cggagatctc gacattcagc tttttgcaaa
     9241 gcgcacttaa gatattgatg cctggcgaca tattcttgtt ttcgatattg ctgacagttg
     9301 cttgttcaca aatgccttct gctaattgta cttgtgttaa cccctttttc tttcggtatt
     9361 cttttagtaa tgctccatct actttaatca aaacaatttc cttcccttta aaaaattatt
     9421 tctcctttat atcatacaca aaaaaaagtt ctaaacaacc aaaaatgttt agaacagaat
     9481 aaaaatttta attttaaaat aatattcaaa ttaaacaact ggcgtatctg ctccttgatt
     9541 tctttttgtg ttatctgaat aattatcgtt tacagttata acacatagtg aaaattgtca
     9601 tggcgagaaa gtctaagaat atttttaaac ataactattt ctttcttacg tgtgtataat
     9661 gacgacagca cacctatcaa ttaatataat tattttattt ttttatgttc tcatgtttat
     9721 ttttaatgac acaacttctc gtatttatct ggcacaacat ataaatcgat caaaaaattg
     9781 ttgcgtgttt ttttgaaaat tggagtttca acttgattac aaaatttaaa tttagaagtt
     9841 gtgaaaacag cgagagacag tttaatattc aaaatataca taggctactc gaacgctgtg
     9901 ttttttagta tactagtaag aaacgaaggg atagactcat tccaataagg aggatttcat
     9961 gaagacaatt ctttttgctc ctgcagtatt caatttagct gagacaacac gaatgattga
    10021 ggttgccaaa gctatgaaag accgttatta ctgtgtattc tttggctttt ctaaaacttt
    10081 tgctcctttg atcacagaaa gtggctttga gtatcattta ctagcgccga tccttacgaa
    10141 aaaacaagaa gaacaaatca taaaatttga ccagttgcgt acacttaaaa acccttttac
    10201 caaagaaatg gttgcctctc gtatcactag tgaacagagt ctgattgcta agtttgagcc
    10261 tcgactgatt gtgacaggtt cgaatgtgac aattttttta tcggcccgca ataaaaagat
    10321 tcctttagta tatgttaaac cctatgcttt gagtgaacct ttttttcaat cagagcatgc
    10381 gctgatgccc cacgagttac gtttcttcgg ccctttaagc aatccccttt ggcactggac
    10441 aaaaaagcgt atagtaaata cccgatggat ccccaatgcc ttcaaacacg ctgcacaaaa
    10501 agaaatgctt cctttaccaa aaacaagttt acaaatgatg gatgctgatc taaatctcat
    10561 cacaacgcct aaactatttt tggaagaaga acagctgccg gaaaattatc gaatcgttgg
    10621 tccaattttt gctaagctca gtacttcctt gcctgaagaa gtgctaactt ttatacagcg
    10681 aaagcaacaa gaaagaaaac gtattatcta tttcgcaatg ggtagttcag gcaatccgcg
    10741 tatgttacgc aaaattttgt tcttcttaca aaaacaaccg gatctagcgg ttattgcacc
    10801 tattgactat ctgcttgatg acattcaatc tgactcagaa gatcttttaa taacagaata
    10861 tctgtctagt catcttttgc atgaattcat tgatttttct tttatccacg gcggggaagg
    10921 gaccgtccaa actgcctgtg aatccggcaa accgtttatc ggtgtgggga tgcaatttga
    10981 gcaaagctgt aatattgact attgtgtaaa atacggaaac gcaatcgccc tcacaaaacc
    11041 agtgactgaa agaaaactgc agattgctct gcgcgaagtt tcttcaccca agattcgtca
    11101 gcaggcacgc acgctgcaag ccgtttttcc aataaatggt ccacaacaag caattcaaga
    11161 aatcgaaacc ttcctgagca accacccaat atagtttcaa gtagaaaaag cttttttaat
    11221 agagactcat cagtaatttc gctattcgag acccagacag tgaaaataca aaaaaagtaa
    11281 atagtggatt gaaaagttag agttacttaa attacggtgt ctaaaaaaca caaaatcatc
    11341 cttaaaataa aagatctgac gctatccaaa tgagcatagg tagcgtctgc catttattct
    11401 tagtcaaatt ctttgacacc tttctctggt tggatcatct caacgtttct tcaaaagaaa
    11461 gaccataata attaggcaaa tacattttct taaaatgtgt catatagatc tagaagcatc
    11521 gctttgtaga gatttgtgcc gctagtcccc tcaacaatta ctaaatcaaa tgtaggattt
    11581 accactttat ctttgataat caacctccct cttctgttga gacaatgatc tcgcaatccg
    11641 atgcatactc ctatataaac tgccagcaat ccccacatat tgagtagatc atgtcagaat
    11701 gttctatgtc gtcagcaacg atacactaaa agtcacgttc accatgactg attgctattc
    11761 ccagcacaat tatttctata cacgtttcat gatttgaata aacactcaca tcatacatat
    11821 taccattcat acaccatact gcagccccta ctatatggtt gtaagctccc aaatctatat
    11881 ttgacttgat cactttggta gtagatctac taattctaaa tccattgctg ataaaatttc
    11941 cattttttcc tccttctttc tgccaaaaca gttaaaacaa taataatctt tatgtaaaaa
    12001 agggctgtga caaaagcctt gtcacagtcc catattccga ataaacggtg gtcaaaagct
    12061 tgcatctgcg gtaataagtt caaccaaacg aaaaataagc caacatttca caaaaattta
    12121 agaagcaatt ttcgtaaatt tcttcttatt tcttgaagct aaccacttct gttacaacca
    12181 cttttagaac atttaaagct tcgatgtgac aaaccatcta tgctgctcga tcataaaatt
    12241 gatagcaaaa attccacaaa gtacatatgt cccccatgaa aacaaaacat tcatatgcgg
    12301 tacaatgatc tttggaagaa tcacgatcaa aaatgatgcc agtgcccaaa tccatcgatt
    12361 gatttgaata tgatatgtag ctagcatatt caatataagg tgactagccg ttaataaaaa
    12421 gataaaaaca aaaaataaaa tcagccaata cattgtttcc caccttctaa aaaccaaact
    12481 tagacagtct ggacttcgtt ttctccacga ctttgttcat ctgacagcat ctttttatca
    12541 taagctttta caaatggata atagataata aatgctatca atgtgttgat catgatcaag
    12601 ataacaacac gccagtcatt tcctgctgaa agataaccgc cgattggagc tggtaatgtc
    12661 catgttggca aaacagtaaa tctattgacc atttctaatt tgactgcaaa ataactgata
    12721 atagtcaaaa ctaaaggaac taagttgaac gggatgaaca tgtaaggatt caacatcata
    12781 ggagctccaa aaattatggg ctcattgata ttgaagatac caggaataat agaaaaacga
    12841 ccaacctctt tcaagaattt tgatttacag aagaatataa acaaaacaga taccacgatc
    12901 gttgctcctg caccaccaat tgttaccgtc cattgataaa actgttcagg agcgatatag
    12961 ggtaattggg tggcagctgt accatctgcc aatgcctttg cattctcatc caacatgatc
    13021 agccacattg gacgaacaac agcccctaca atactcatgc catggatacc aaatgaccac
    13081 aacaaatgga tcaaaaacat tggtagtaaa acaccaagca aattattccc taaaatattg
    13141 gttaaaggct cgaataacga taataaggca gcgtttacat caaagcccgc gacgcttcga
    13201 atcagccaag taattgttaa aatgactgca cctggaatca atgcctcaaa actacgggca
    13261 acagcaggtg gcacttgttc tggcattttg atagtaatat ttcgattttt aaagaaacga
    13321 tatacttcca ctgcaaaaat catagataag atccccgcaa acatcccaga tgctcccaaa
    13381 gaactcatag gtaagaccca gccaagaggt gcctcttcag gagttaaaac gtttacagga
    13441 acttgtaaca taaagaacgt tcctaaagat aatataccac ctgtcacact atctaatcca
    13501 tatgatttag ctagatccga acccataaca aaagttgcat aaatagccat caatcccatc
    13561 gtcatcctaa agggtaaaac aatcgttgcc gaataagggg caataaattc attccatcca
    13621 ggaaacggta aattgataaa gaccataaaa aatgtcccaa tcaaaatcaa tgacaatgtt
    13681 gaaacaaccc cattacgaat tgctaataaa tgacgctgtg taccaatttt tgccattggt
    13741 gggacaagtt tctcttcgac ccatttcatc agcttttcca ctaaaaccac tccttttcaa
    13801 taaaatatga accattcatt gcatgacaac gcaaagaatg ttcataacta attatataat
    13861 taagaaattt tatttcaata tattctctta aataatcaaa tttattttta agaatcctga
    13921 aaataagttt tctaaatcat aggagcaaca aaaaaattcg ataattaaca aaaatcaaaa
    13981 tgattaaatc tagtgattga aacatatcat ttcaaaaaaa ttttgtaaat tcacccataa
    14041 atatcaccct atttaacttt tttcatcgta cactaaaatt caaaacaact atctttaatc
    14101 tttacttctt ggttttgttt taataaattt ccttcaaaaa tgcctggaga aactacaatg
    14161 atcaccgtat ctttttgtgc ttgctccaac ttcttcaaat ctataatagc taacagtgtc
    14221 ccttctttta cctcctggtt actttcaacc cgtatctgaa acggacttcc ttcgagttca
    14281 actgtatcta aacccatatg gatcagtatt tttctgccag attcactttc gatcgttatg
    14341 gcatgtcggg taggaaatat attatcgatt attcctttaa ctggcgcatg gatatatcca
    14401 tcatgattcg ttatagcgta tccaactcct atcatttttg aagaaaagac ctcgtctttt
    14461 actgctgtta atggaataat ttgaccacta gctacagctt ttagaatgtt tcttgtttta
    14521 aagaaatgca ctttttatca cctctacttt aatgctggcc aatatttttt gtttactttg
    14581 atcatttcat ctagtatttt ttttgcgact ctggcatttg gtacggtttg attcaatgta
    14641 aatgcttgta aagctttttg ataagaacct tcaaagtaag catctactag taacttctct
    14701 actgcaactt gtgcctccat caatccttta tgaaaatcac tgattggttt tcttagtata
    14761 attggctcca caccagttgc accaacatat gccggtattt caacaactgc atctgaccgt
    14821 agattcggaa tcgccccttg attaggaacg attagcataa agcgacgacg atcatcattc
    14881 aacaaggaaa tagctatatc gacgatatat tgtccatgct ccccaaaatt aaagttcaat
    14941 acttcttcca caataccgcg gcgtatttta tcagccattt ctttggtatt tttctctcgt
    15001 ccatccatga ccatattagc acgtgtatac tctttgtcag tatgctcaac gaccatatca
    15061 ggatataagt aatactctaa gtaattattc gggatattac ttggaaaatt tttcgtgatg
    15121 actgacatca tttgaaaggt tttttgccaa gttttatctc ctatattaaa atcggcgact
    15181 tgcatttctt ttgttattag tttttcaata atctctggca taatttctcg ttgcaattct
    15241 ttatcataaa tcgatgtata ccagccaaag tggtttaaac cataatacgt aacgatccaa
    15301 ttcttacgat cataaccgta attcactgca atcgtttctt caattgaaat agtcatatca
    15361 caggcattaa tcatttttac attcggatat ttacgacgaa cagcttcaga aacgatcgac
    15421 tctggatttg tataattcaa aatccatgct ttgggggcat agtcttgtat atgatcgaca
    15481 atttccaata aacctttgat tgagcgcatc ccataagcaa atcctccgag tccacacgtt
    15541 tcttgtccta ctaatccatg tttaagtgga atcttttcgt ccttttctcg catctccaat
    15601 cctccaacac gaatttgtga gaaaacaaaa tcgcatcctg taaaagcaag ctttggatca
    15661 tccgttgcac gaatgcgaat atttgaaaaa ccttttcttt tcagcataaa tttaacaatc
    15721 aagtacatat ccatatttct ttcttcatca atatcatata aacgtatttc agataatggc
    15781 aaccgttttt cattgttcaa aatggcttga acgattcctg gtgtatacgt actcccgcct
    15841 ccagctatag tgataatctg ttttttcatc atcatctttt cctttctatt cttctaataa
    15901 tttattgaat tctgccataa cataagtcac ttctaagccg atgataattt gataattttt
    15961 ttcagttggt ttaatgatcc cgcttatccc taattgcttg attcgctgtt cctcgaccat
    16021 ggtgggatcc actacctcta cccgtaggcg tgtattacaa ctatagacat ccaggatatt
    16081 ggcttttcct ccaaacgctt gtagcatttt tgttgccata tattcataat ttttacttgc
    16141 taaccctaac tctgtttctt ctaaggttac ttcattgcca aactctgcct tagcttcacg
    16201 tcctaatgtc tgaatgtttc tcttattgat caaataataa aaagtaacgt aatacaaggc
    16261 aaaaaagaaa attccgattg gaagaatcaa aatagcattg gtagcgattc gaaaattgat
    16321 gagataatca acaatacatg ctccgaatgt aaaaccaatt cgtatattga ataagtaaca
    16381 gacaatccct gctaaaccgg caaaaagtgc atggatcacg tacaacatag gagaaagaaa
    16441 catgaaacta aattcaacag gctcagtgat atttgcaata aatgaagttc ctgcaccgct
    16501 tcccatgatt cctttgacca tctctttatt ttctttataa gctgctttat agatagctag
    16561 acatatagct ggtataccaa acatcattac tacaaaaaaa actgataaaa acagtccagc
    16621 ctgaggatct ccgttgataa agcgtgtcat ctctcctctg aaaacttcct tgctaggaga
    16681 tgtatagctt cctaaatcgt aataaatata cgtattcaac acatggtgca gtccaaaggg
    16741 gatcagcaaa cgattcaaaa acataaatat ccccacacct aatgctccca tatcaacgag
    16801 cagttttgca aaattatcaa tcccaatttg gatgagcggc cagataaagc ccaaaaaaac
    16861 agctgcgatt gtttgtagaa tcatcaccat agtcaatggg aatttttcgc ccgcaaaata
    16921 agaaaaaact actggtaatt tttgttcttt aaagcgatta tacgtccatg ccgctactaa
    16981 accggaaata attccaccaa aaatccccat attcacttct gggtccataa tttctaagcc
    17041 ttttttcaag gtagcaatcg ctaaaaaacc agtaagtgca ggtattcctt tgtccttatt
    17101 tttgacaaaa ccgattgcaa ctcctatagc aaataagata tccaagtttc caaaaacggc
    17161 atttccagct gcaccaatta ctgggatgtt cagcatatcg tcagctgata aacgcatgat
    17221 caatcccgct aatggcatcg ctgcaactgg aagtagcatc gctcttccaa gcttctgcac
    17281 atttgattta acattcattt ttattcctcc tctattaaat atcctcatat gttactaact
    17341 caatcaaagg atcatttttc actttttgag caaatgctga atgaattaaa aattcgactt
    17401 ggtgggtacg aggatacaca aaacttgaac catttaacaa ttcctgatcc aaatacgctg
    17461 cgtgacacat gacttcgatc gttttatctt ctctcaatat catgagtaaa cttgccaaat
    17521 attcttccac actcgtattg atttgtcgtt ttttttcatc agtttctcct acgacatcga
    17581 aacaaggctc aaaataatcg acatgacgaa cttcatcttt tcgttcaaag ttgctccgaa
    17641 ccggcaaatc atattttttt gctaatgtaa caactacctc ctgattttca tccaaagtat
    17701 gagtatgatg atgactgtct aaatgagttg gtatgatccc cgcatgataa attttttgaa
    17761 tctgagcatt ccattcctga tacagttgat ctttgtcaat gttaaatgga tgctgataat
    17821 aagataagga tctgaactct ccgctttcga taagagtatc gacagttttg aggagtggtt
    17881 tgccacacgt caatgttaaa tggactccta cacctagcct aggcatttcc tttcttagtt
    17941 tcacagcatg ctcgaaaccc ggcatatttg ccatcaatgt agttgatgtc aaaattcctc
    18001 gctgataagc atccataatc ccataattca caccatggct atagccaaaa tcatccgaat
    18061 taaaaatcac ttttcccatt tcattctcct tcttttttct actaaaatag gcgcaaccaa
    18121 ataatcatag tccatttact atgtgtcatt tggttgcgcc tatagatagt tacgccctta
    18181 ttttaataga ttcttgctag atgaattgcc aaatagatga tttcattttc ttgaatggca
    18241 agttgaaatt gctgttcgat gatatcttta atctttagtg ctgtacaata cgctttgcta
    18301 tgtttaaatg tttcagtaaa aaaaccaata ttttctactg gcgtttcttc ctgataaatc
    18361 aagcgtttaa ccaggaattt gatatgaacc attaagcgat gataagtcag tgattcttta
    18421 tcataaacgt ttgaatagga tttttctaga tgggacaaaa tcgtggtcgt catttctact
    18481 atttttaagc tctttaaact attaggctct cctagctcag cattaatgat atgcatagtg
    18541 atgaatccta cttcatttga ccctaactcg gtttgatatc gatgattgat tcgctcgacc
    18601 acttctactg ctatatcata ttcatttgga taaaggaatc tcatttctgg caacataatt
    18661 gctgtgacat ctctatcttc tctttttcgt tgaatagcaa aataaatatg atctgtcaat
    18721 gtaatataga tatttgaatc taatggagag gagatcctag ctgttatcag ttcaatcact
    18781 tcttccgcaa aatccataaa ttcgattgga atctgcgaga ctatctcact gaatcgtttg
    18841 atcttttctt tagaatctaa tacaaataat ttgtcagcat cctcaacatc aaatggctta
    18901 ctcactttag cagaaaagcc aatccctttc cccatcgcaa ttttttctat tccatcgtca
    18961 tcgatcaata cagcattatg attcagcttt ttcatcacat acaccgttaa ttcacctcct
    19021 ctatatgata aaacaaaaca acttgcagac tccttcatac atatttttgt ctccaaacaa
    19081 tttttaaaga caaaaaacga cctaagaaag aaccctttct taggtcgtgc ctaattgaat
    19141 agtagcaccc tacataattt acaaaaatag tttaacacat aataaaagcg ttatcaatat
    19201 cttttttact cgttttatga taatacttgt atcgaattga tttggaggta attacataaa
    19261 aacaaaaaaa tatagcaact ctctataaga aagttgctat attgataatt ataccggcgg
    19321 ccggggtcga accggcacgt cctcaacgga cactggattt tgagtccagc gcgtctgcca
    19381 attccgccac gccggcaaat ttaaaaataa taactggggt agctggattc gaaccaacgc
    19441 atgagggagt caaagtccct tgccttaccg cttggctata ccccaagaat aaagggcgaa
    19501 tgatgggaat cgaacccacg aatgccggag ccacaatccg gtgcgttaac cacttcgcca
    19561 cattcgccat cataaaagat gttatttaat ttctcaataa aaaactaaga tgaagacatc
    19621 ttagtcaata ccggcggccg gggtcgaacc ggcacgccct caacgggcac tggattttga
    19681 gtccagcgcg tctgccaatt ccgccacgcc ggcaagttga tttattagta ctaaggcggt
    19741 aaccggattt gaaccggtga tgaaggtttt gcagacctct gccttaccac ttggctatac
    19801 cgccattata ctattatcaa tctataaaat aaagaataaa ttatagaaac tggggtagct
    19861 ggattcgaac caacgcatga gggagtcaaa gtcccttgcc ttaccgcttg gctatacccc
    19921 aagaataaaa agggcgaatg atgggaatcg aacccacgaa tgccggagcc acaatccggt
    19981 gcgttaacca cttcgccaca ttcgccatgg caggggcagc aggaattgaa cccacactaa
    20041 cggttttgga gaccgctgtt ctacctttaa actatgcccc tataaaatgg aggagagtgg
    20101 attcgaacca ctgaacccta aggaacggat ttacagtccg tcgcgtttag ccacttcgct
    20161 actcctccat ggtggcgcgg gacagaatcg aactgccgac acacggagct tcaatccgtt
    20221 gctctaccaa ctgagctacc gcgccatatt ttaaaattat taattgaata acaagaacga
    20281 cggttccgac gggaatcgaa cccgcgatct cctgcgtgac aggcaggcat gttaacccct
    20341 acaccacgga accagttcat tatggaggtt aacgggatcg aaccgctgac cctctgcttg
    20401 taaggcagat gctctcccag ctgagctaaa cctccaacat tcaattaaaa tgacccgtac
    20461 gggattcgaa cccgtgttac cgccgtgaaa gggcggtgtc ttaaccgctt gaccaacggg
    20521 ccaggtaaat ttgctacgga gagtaaggga ttcgaaccct tgagacagtt tggcaccatc
    20581 tacatgattt ccaatcatgc tccttcggcc tctcggacaa ctctccagag tgaaagaagc
    20641 tcaatactct ttctcaaaat gag
//
LOCUS       DACKAC010000048        20417 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.048, whole genome shotgun sequence.
ACCESSION   DACKAC010000048 DACKAC010000000
VERSION     DACKAC010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.048"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(220..972)
                     /gene="xth"
                     /locus_tag="I0B46_001872"
     CDS             complement(220..972)
                     /gene="xth"
                     /locus_tag="I0B46_001872"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAR2084154.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            1085..2005
                     /locus_tag="I0B46_001873"
     CDS             1085..2005
                     /locus_tag="I0B46_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAR2084155.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2078..3013
                     /gene="murB"
                     /locus_tag="I0B46_001874"
     CDS             2078..3013
                     /gene="murB"
                     /locus_tag="I0B46_001874"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAR2084156.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            3311..4789
                     /locus_tag="I0B46_001875"
     CDS             3311..4789
                     /locus_tag="I0B46_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAR2084157.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            5066..7126
                     /locus_tag="I0B46_001876"
     CDS             5066..7126
                     /locus_tag="I0B46_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084158.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            7209..7778
                     /locus_tag="I0B46_001877"
     CDS             7209..7778
                     /locus_tag="I0B46_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAR2084159.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(7953..8759)
                     /locus_tag="I0B46_001878"
     CDS             complement(7953..8759)
                     /locus_tag="I0B46_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084160.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            8897..10411
                     /locus_tag="I0B46_001879"
     CDS             8897..10411
                     /locus_tag="I0B46_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAR2084161.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(10814..11797)
                     /locus_tag="I0B46_001880"
     CDS             complement(10814..11797)
                     /locus_tag="I0B46_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAR2084162.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(11802..12449)
                     /locus_tag="I0B46_001881"
     CDS             complement(11802..12449)
                     /locus_tag="I0B46_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAR2084163.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(12534..13166)
                     /locus_tag="I0B46_001882"
     CDS             complement(12534..13166)
                     /locus_tag="I0B46_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAR2084164.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(13182..13733)
                     /gene="coaC"
                     /locus_tag="I0B46_001883"
     CDS             complement(13182..13733)
                     /gene="coaC"
                     /locus_tag="I0B46_001883"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAR2084165.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTVIEQVMLTLNERNA"
     gene            complement(13730..14467)
                     /locus_tag="I0B46_001884"
     CDS             complement(13730..14467)
                     /locus_tag="I0B46_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAR2084166.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(14464..15453)
                     /locus_tag="I0B46_001885"
     CDS             complement(14464..15453)
                     /locus_tag="I0B46_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAR2084167.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            15711..16253
                     /locus_tag="I0B46_001886"
     CDS             15711..16253
                     /locus_tag="I0B46_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAR2084168.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            16270..17355
                     /locus_tag="I0B46_001887"
     CDS             16270..17355
                     /locus_tag="I0B46_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAR2084169.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            17367..18173
                     /locus_tag="I0B46_001888"
     CDS             17367..18173
                     /locus_tag="I0B46_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAR2084170.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            18174..19001
                     /locus_tag="I0B46_001889"
     CDS             18174..19001
                     /locus_tag="I0B46_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAR2084171.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            18998..20071
                     /locus_tag="I0B46_001890"
     CDS             18998..20071
                     /locus_tag="I0B46_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAR2084172.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
BASE COUNT         6730 a         3582 c         4086 g         6019 t
ORIGIN      
        1 atctgcggta ataagcccaa ccaaacgaaa aatatgagga actatttttg tttggctgtt
       61 cttattactt gatgcaggac agctttttca caatctctcg ataaacagtg ttcaaaagcc
      121 agtttctcgg tattatgcca aatttatgca aaatgtggaa agccattttc acaaaatttt
      181 tcttaatact cgaaactaaa cgactttttc acaacctctt tatttcaaat ctaattctac
      241 tggacaatga tcactgccca taatgtccgt atggatcttt gcatcagcta gtcctggctc
      301 taaatctttg ctcgtcaaaa agtaatcgat ccgccaacca gcattgtttt tccttgaatt
      361 gaatcggtag ttccaccaag agtagacacc ttcctgggtt ggataaaaat aacggaatgt
      421 atcgataaac ccgttatcca acagtcgaga aagagccgct ctttcttctg gagtgaatcc
      481 agcatttttt tgattcgttt tccagttttt caaatcaatc ttttgatgag cgacatttag
      541 atccccacac acgatgactg gtttttgttt ttttagattt tccaaataat tataaaaggc
      601 ttcttcccat tctaatcggt aatctaaacg ttttaattcg ctttgagaat ttggcgtgta
      661 acaggttaca agaaagaaat cggaatattc caaagtaatc agacggcctt ctgtatcatg
      721 gatatcgata cccattccgt aagtggcatt caatgctggt tttttagcaa aaatcgctgt
      781 gccggaatag ccttttcgtt ccgcgtaatt ccaatattga taataacctg gaagatctaa
      841 atcaatttgt cctgcttgga gtttcgtttc ctgcagacag aaaaaatcgg cgtctaactc
      901 atgaaaaact tctaaaaaat ttttatttac gatggcacga agcccgttta cgttccaaga
      961 gataaatttc aataaaagca cactccttcg aattaatttc cttacctttg cgatactatt
     1021 gtacctcatt ttagtaaaaa atggtgtaag atgaaagtaa ctaaatgatc caggaggaaa
     1081 gtagatgaag attggtgtaa tcggtgtagg gaatatcgca gaaaaagcat atttaccaac
     1141 ttatgcgaaa aaacaaggaa cacttgattt ttattttgca acaagaaata aagaaacaaa
     1201 agaaagaatt caaaaagagt acgggttcca acatctatat gaaacgatcg atgagctttt
     1261 agaagaaaaa atcgaagcat gtatgatcca cgcagcaaca aaagtacatt atgaattagc
     1321 caaaaaatgt ctagaaaatg gtatccatgt ttatattgac aaaccactga gtgtccatct
     1381 aaatgaagtt cgtgaattgc aagaattagc tgataagaac caaataattt tgatgatcgg
     1441 tttcaaccgg cgctttgctc ctatggtaga agaattgaaa cgtatcccag ataagcggtt
     1501 gatccacctg caaaaaaatc gaatagcggc aaaagaaacg acagagttcg tcctttatga
     1561 tttgttttta catcttattg atacagctgt gtatttatta gatgaaccca ttttgagaac
     1621 aaaataccag ataagagaaa caaaggaatt tttggaatat gccattttac aattagaaac
     1681 acaaaatcaa acggctattt taacgatgga tttagcaagt ggcgctaata tagaaaaata
     1741 tcaagtaaca agtaaaaatg gtacttatga agtaaacgat ctgacccagc ttgtcattca
     1801 agatgctgat ggaaaaaaag tgaaagaatt tggcgactgg acaaacacgc tagtcaaacg
     1861 cgggtttgag ccaatggtag aggccttctt ttctcagatc cgtttaggta aatcggaaac
     1921 agaattgctt aaacaaaaag aagttgtaag aagtcatgaa ttatgtgagg aagtgcttaa
     1981 agatcactac cgtcatcaac tttgaacaag actggcaaga ttgtattaag cgtgatagaa
     2041 taatgaggac gtttgagaaa agaaaggaaa atcctcagtg aataaagaag aaatagttga
     2101 aagcttagcc acgattacta tattgtttga tgaaccattg atgaactata cgtttacgaa
     2161 aacaggtgga ccagctgatg tccttgcatt cccgaaaaaa caagaagaag taaagcaaat
     2221 catcgattat tgccgaatcc atgatatacc ttggatggta ttaggaaatg caagcaatct
     2281 gatcgttcaa gatggcggga ttcgcggtgt agtcatcatg ctgactgaaa tgaaacagat
     2341 tcatgtcaaa ggaacgatgg tcattgcgga agcaggtgcc tcactgattg atacgactta
     2401 tgcagctttg gcagaatcat tgactggatt tgaatttgct tgcggcatcc cgggaagtgt
     2461 cggtggagcg gtttatatga atgcaggtgc gtatggcgga gagatcaaag atgtttttgc
     2521 tgaagtggat cttttgcttg aagatggaac attgaaaact ttgacaaaag acgaaatggt
     2581 attttcttat cgtcacagta aagtccaaga attacgtgct atcgtattgg aagctcgatt
     2641 cagtttgcaa acaggggatt atgaagcaat caaagcaaga atggatgagt tgaccgaatt
     2701 gcgtcaatca aaacagccat tagaataccc ttcatgcgga agtgtcttta agcgcccagt
     2761 aggacactat acaggtcagc tgatccaaca agcaggattg caaggcctga aatggggcgg
     2821 tgcacaagtc tctgaaaaac atgctggatt catcgtcaat atcgatcatg cgacagcgac
     2881 agattatatt gaattgattg cgcacatcca agaagtaatc aaagaaaaat ttgatgtgtc
     2941 tttagaaaca gaagtgcgga tcattggaga aaaacaagca atatcagaaa caagcagaag
     3001 tttagccaat taataagtaa tggaacttaa aataagtaaa agagcaggac ggtggccgtc
     3061 ctgctctttt ttgtggttgt ttacgcaaat ggcgcaaatc ttgtacattt taacacaata
     3121 aaacaaaaaa tatagaatat ttcctcataa atttttccat tttttgcaat attctcaaaa
     3181 aaaagataaa aaatgagaat aactgatgaa aaaacaagca taatccatca taaaatcagt
     3241 ataggtgaaa agatgaactt tcatctgcat atcaagtaat tggtatgagg cattttttga
     3301 ggtgtaggat atgaagttgg attaccttat agaagaggaa gatagagaaa aactgctgct
     3361 agctagatat ttagaatttg agaaaaagaa ttatgtagtg ctgcctaagt tatatgaaac
     3421 attaggtttg tctaaattta aaacaagaaa ttatatcata gaattaaata aagaatttac
     3481 tgaattcaaa acaaagccag agatccaaat ttcagaagaa gcagagatcc atgtcgaacg
     3541 tctgaatttg acggatatca aaaatttaag aagaatctac tttcaacgat caaaaattgc
     3601 tcgactgtta acggagatcg tggattcaaa agtaacgatc gaaaaatttg cagagaatca
     3661 ttttttaagc atatcaagag cctatgtaaa aagaagagaa ttagtctctt ttcttgaaca
     3721 gcatcaaata aagttaaaga aaaacatatt agttggggaa gaaataaaca tacggaacgt
     3781 attatttggg gtatatttcg gggtctttaa tggtttttct atgccattta gagaaagcgt
     3841 catgaatgaa gcaaagagta tcgtgcaata ttttgaatat ctgttcaatt taaaactgtc
     3901 tgcaacaaaa cgaatcaaat tcaatttgct aaccactatt agtttgttga gggcctatgg
     3961 gaatcaacca atcggaaaac gtttcttttc agatgaatac tttaagagct cagaaggaat
     4021 gaagatcatc aaggatttat cacatatttc ttttcttcat atccctaaag agcagatcat
     4081 tgatgagatt tcttatatca tgttgttttt gatcacggaa gaagaatttg aattagatgg
     4141 cgcatttgga cttgcggaag ctcattttca tgatatccac atgattagtc aaaaaatgtc
     4201 agtggaattg attgatcaat taccattttc gattgaaatc tctgaagaag aaagactata
     4261 tatacgaaaa gaacttcgta tgggtatgat gcatgtcaat cgaaaaaatt ggatattcga
     4321 ctttgagatg actagcttta cgtctaaaaa gcagctacag tttttcttcg agacgtatcc
     4381 aatattcagt acagcaatcc aaaaggtcat acaagagaat aaacgctatt tcttgggaaa
     4441 agatgaacgt ttcatgattc gcttatttta tgattatctc tttttattgg tcggtatttg
     4501 tccggtatca tattttgaag caccgattca tgtctgtatt gatttttcgc aaggatctgc
     4561 ttataccgat tacattattt ctcaagtgaa aggatttaaa aattacaata ttgtgattga
     4621 gagtcgaatc acttctcgta cgaatattta tttatctgat tgtttacttg aaaaatttaa
     4681 taaagaacag ataatttgga aaaatccacc gactccagat gactgggaat attttggaaa
     4741 taccatcatc agagaaaaaa atagacattt aactgaaaaa atcagctagt atttatggat
     4801 ggcagctttg tctcacatat ttattagctg atttatgttt ttttcaaaga aaaggtaact
     4861 agatataact agatataact agatatatta cttaacaaaa ttaaaaagta cataagttat
     4921 tattccgatt ttcttgaaac aataaaaaat caaaaaaaat actctcctaa tagtccatct
     4981 tcttttgatt ttatttatat aatgaaatca tcttatgaaa attactactt catgtttaga
     5041 caagagtagg gaggactaag aataaatgtg ttcaaaaaga cgttcaaaac aactaaataa
     5101 acacgcttgt cgtttagata aggaacggtc gtgttctgaa aaacactacg ttaagtttat
     5161 gatgcgcacg atgctgatca gtttatctgt aggtgtctat atgcatgtac ccgttcaggc
     5221 aacgacacaa accatcaagg tcgtacgtac ctctgtttct gagaccactg attcccctat
     5281 cactattact catactttca ctgatgcaaa tggaaaacag ctacttcctg cattgacttc
     5341 ctataataaa gtcagttatc ctccaacgat aaaccactac tggttaattc ctaacaaaac
     5401 attatggaca tcggaaaccg gaagcagtgt gccgctttct atcttttccg atatgactga
     5461 tcctacaaaa ggtgatcagt taaaacagat cactgatatt ttgaatcatg ctttgacaga
     5521 tataaaggac ggtggaagcg cacaagtttc ttatgtatat gaaaaagatt attcacagtt
     5581 tgaaagaaag aaaatcgatg ttctggcagg gacaaaagtt tcgatatctg atctggtaga
     5641 aaggctgaag aacgcagatg gaacacaagg agatatgtcc aaagtaattt caaacaatgg
     5701 gaatatcgtg gatacgacta atacaggaac atctactatt tccttgtcct attttgatcc
     5761 aattgcttta aaaacaatga ctgcatctgc tgtgatccat gttcgtgctg atcaaacagc
     5821 tatcaaagga aaagatcttc acttaagaac aggagagagt tacgccccta aagatcttgt
     5881 tgaatatgta acaaaaagcg atggaacttc aggaaataaa gaagaaatca aaatgaacgg
     5941 gacagtagat actacaactc cgggagaata tcccgttgta ttaacttata tagactcgaa
     6001 aacatcaaat gtggcagaaa caacagctgt agtttatgta gaagcatctg ctgcttcatc
     6061 gactgtcatc actcatttta tagatagtca aacaaataaa gaacttcaag cagaaacaca
     6121 tatgagtagt gtaaatcgta ttcctgattt gcgcctacct gctgaatttt ctgatcgagt
     6181 gccaaatata gatttatcga cttatgaaca aaatgggaaa gtcacttcta tttctgattg
     6241 gatgcaagca aacggattag acagaaaaag tcactggtcg gagatccttg aagtacttca
     6301 ctcagaactg atgaataata ggaagcaatc aggaggggaa attaccttta cttatgttta
     6361 tataccagac aatcgttcat tagaaggtca tgatcttatt gttcatccaa acgaaaaaat
     6421 aaacaaagaa gagttgatcc aatcagcaac aaattcctta ggtaaacccg taccgttaaa
     6481 ggatgtatcg gttacaatca atgaagtacc attggaggat ggttatttga ccaaacaaac
     6541 aggtgatttt acgattatgt atagttatca tgacccgtat tcactccaag aactccatgc
     6601 tcagtcacta ttacatgttt taccattaaa caaacagata gatcaaaaag aacaaaaaag
     6661 acaagaagaa agcttgtcag aaaaaaaaca acaaatggct catgcagttt ctccaacagg
     6721 gaaagtaaaa caaacaagaa gcctagagtt agcaagaata tctagaaaaa tctcagaatc
     6781 taataaaacg ataaggcaag aagctcattt ggccccacaa gtttctggat tggaaaatga
     6841 aattttagag cctaaaataa aacaacaatc agaaaaagaa tttgttgcta aacagtcgga
     6901 agatcagaag gaatcagatg accagaagga aacgaagaaa aaacgcttgt caactattga
     6961 ttccaccgat gagcatctgc agccgcaagt accacttcct agtccgggaa cacccgcacc
     7021 tggtggaggt attccttctt ctgctttagg agatttcatg cgaggtatgg caggaacatg
     7081 ggtatacgct aatcgaaatg aatttgacgc agatgactgg ttctgattta tatctctatc
     7141 tgttatagtc aaataagtag ctgagtcata tgttatttgg cataatggag acgaggaggg
     7201 atagaaaaat gttagataaa acgttacctt acgcagaaat ctggatgaca agaccacttc
     7261 aacaaaatgt tccaatcacg cctttagcgg aaggttttga atttcgattt tatcaaaaag
     7321 aagatgaatc tgcttgggcg gagattgaga cttccgttgg ggaatttcaa acgacagatg
     7381 aagcacttga gtattttcaa caaaactttg ctccttatcc tgaagaactt gaaagaagga
     7441 tgttttttgt agagacaaaa aaaggagaaa aagtagcaac ctgtaccgct tggcgaaaaa
     7501 aatcaagaga gcaagtgtat ccggtttttc attggttagc agtgaaacca gattaccaag
     7561 gaaaagggct cgcaaaagca ttgactgcca gagtcttgca agaatttcct accctccata
     7621 cggacggacc aatctatctt cacacacaga catggagcca tcaggcgatt gctctttata
     7681 aaaaaatggg ttttactttt attgctgaaa atctagacgg cacacctaat ccagattatg
     7741 aaaaagttat gcaggtttta agtaaattag ataaataacg aaatggtgaa ataagtcctc
     7801 agtattggga taaaaaacga aatagtcatt cctcatatct tacttttttg agttaatact
     7861 atcggataac taatggcatg ccatacattc aaaaaagaag gggcagtgac aaaagtattg
     7921 tcactgcccc ttctcttgtt ttttagcatg ttttatcgtt tgattttttt aggaataaaa
     7981 ttcttgatga acgtaaattt ttggtaatcg tttcgactat agaggtaacc aaatacactg
     8041 aaaacaactg ctccaataaa attaacaaat aaatccttca tagtatccat gattcccacg
     8101 tctaagtaac cattttggat gattgtttta tctgttttac catctgcatt ttgactttca
     8161 atgattgtcc gagtgatatc tggaacatga tagacagtgt ttccgtcttt acttaactcc
     8221 acactattga tttgctggat gatcgtatct ttttgcatat cgaattgcag taatttatct
     8281 gctgaaaatt ccacaaactc ccacataaca ccaactgtca tcgaaaaaca gaaggaaaca
     8341 actgctagta ataaaggagt caagttcatg gtttttgtat ttttattgag caaataaaca
     8401 agggaaaagc cgacaccccc agctaaaaat ccgctgaatg catgtagaaa agtatcccac
     8461 attgaaaaat agccataaaa atcacttaac tctcctaaaa ctgttgaact aaaaataaaa
     8521 gataacaaaa tgatttctag catatttgga atctgtatct ttaatatctt ttccagaaaa
     8581 taagggattt caaataaaat taatgtcagt aataaaagca ttgtatttcc ccagtgttgg
     8641 ttccagacct gatccactag cataataacg acgatgatac gtaataaaat atagattccg
     8701 tttgtgattc tacctgcagt ttttgtactg ttcgtaaaaa aggttttcaa atgattcata
     8761 aaataactcc ttcatgtata tccttaatta tactaagaag cgtgtgttta ccaaagctat
     8821 atgacttaaa gaaaaacaga gttgatattg tgaaaaaaat cacttaatat agtataagga
     8881 gaaaaggggg atacaaatga aaaaattagt tgctggttta ttaacagccg ttgcacttct
     8941 tgctatgagc gcatgcggcg cagatcagaa aaatacagcg cagacaagtt ctagtacgaa
     9001 ggaatctgcc gaagtatcat caggcgcttc tgaacaagta tatacagatc caaaagaaat
     9061 gaaagaatct tatgatgtcg tgattattgg agctggtggt gcagggatgg cagcagctat
     9121 cgaagcaaaa gatgctgggt tgaatccagt aattttggaa aagatgcctg ttgccggagg
     9181 aaacacatct aaatcatcaa gcggtatgaa tgcttcagaa acaaaatttc aaaaagaaga
     9241 aggaatcaaa gactcgaatg atgccttcta tgaagaaacg ctaaaaggtg gtggaggaac
     9301 gaatgataaa gaaatgcttc gatactttgt cgatcactct gctgatgcgg tcgattggct
     9361 agatcaaaac ggtatcaaac tggacaactt aacgatcact ggagggatga gtgaaaagag
     9421 gacacatcgt ccgtctgatg gctcggctat tggtggatac ttagtggagg ggttattgaa
     9481 taacgtccat gagcgggaaa tccctatttt tgtcaacgca gatgtgacag acattaagaa
     9541 aaatggggaa acggtcaatg aagtgactgt acatgttcaa ggagaagaac caaaagaggt
     9601 caccggaaaa gcaatcgtag ttaccacagg cggttttggt gccagcaaag aatttattga
     9661 aaaatatcgt cccgatttag ctgattatat tacgacaaat caagaaggat caactggaga
     9721 tggtatcacc atgattgaaa aattaggtgg acaaaccgtt gatatggata aaatacaaat
     9781 tcatccaact gtgcagcagg atgagggggt cttgattggt gaggttgttc gcggagaagg
     9841 agcaatcttg gtcgatcaag aggggaaaag atttgtcaat gaaatggata caagggataa
     9901 agtatctgca gcaattacag cattgcctga aaaatcagct tatttgattt ttgaccaagg
     9961 cgtgcgtgat cgtgccacag ctattgaatt ttatgaccaa aaaggatatg tcacaactgg
    10021 aaagacaatg gaagaattag cagaaaaaat cgatttacca aaagaaacac tagcaaaaac
    10081 agttgatgga tggaatacgg atgttgcgga gaaaaaagat gctcaattca accgtgccac
    10141 agccatggaa catgacttat cgtctccgaa ttattatgcg atcaaaatcg caccaggaat
    10201 ccattacacg atgggcggtg tgaaaatcaa tacgaacact gaagtattag ataaagaaaa
    10261 ccagccaatt agggggttat atgctgctgg tgaagtcaca ggtggcttac atggaaacaa
    10321 ccggatcggt ggaaattcag ttgcggagat cattattttt gggcgacaag cgggcataca
    10381 agcagctcga tttgcttcgg aaaatgaatg aaatagaacg taaataaaaa gaggttgtga
    10441 aatcagcgtt ttgagctaat accgagggtc agctgattga acactgttta gtgaagaggt
    10501 tgtgaaaaag ctgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    10561 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttattcgga
    10621 aaaagagcgt gggacaaaag taaagaacac ttttgtctca cgctctaaac acgtataaac
    10681 ggcggaagca gaagaaattc cttcggaaat aagctgaaat tcacaaaaat ttgaaaagca
    10741 attttcgtga atttcttctt atttcttgga gttaaacgct tctgtcccag cctctttttt
    10801 gatcagcatg attttattca gttatcttca aaatgatttt tccgatgttt ttgtttgctt
    10861 ccatatattc atgcgcttgt ttgactgcgc tgaattggaa tgtacggtca atgattggcc
    10921 gaatcttgcc attttctaga aaaggcccag ctttttgcat aaaagtttgt gcgagctctg
    10981 ctttatactg atcacttcta ggtgtcaata atgtaccaag cagttggaca cgtttcccaa
    11041 tcaatacacc aaggtctatc tcaggcacga tggtcccacc tagcatacca atcaagaccc
    11101 aacgtccatc aacggctata gaagctaaat tcttttccca gtaagaagcg ccgatgaaat
    11161 ccaaaataac atcgactccc cgaccttcag tgatttttcg tacctcttct tgaaagtctt
    11221 gttttttata gttgatcaat tcatctgcac caagttcttt acaaaaggcg agtttttctt
    11281 ctgatcctgc agtaacgatg attttcgcgt gagaaagctg tctagctaat tggatcgctg
    11341 ctgttcccac accacttgct cctgcatgga tcaagacagt ttcgccttct tgtaattttc
    11401 caagccagta aagtgtttga taagcagtca aaaaaacttc tgagattcca gcagcagaaa
    11461 catagtctag cgtatcaggg agaaggattg cacgatcggc aggcatcaca gcatattcgg
    11521 cataaccacc tagattgact aatccgtata cttttgtccc aggagtaaaa gtggagatat
    11581 cacttttatt ctcaactacc tctccagcta cttcgactcc caaaataggg tagggaggtt
    11641 tcaaagagac tgattctctt ttcataatat ctgttcgatt gacagctgct gtatggactt
    11701 tgatcagtaa ttcgccttgt tttggtttag gaacaggcac ttcaatttgt tctaatacat
    11761 ctacagaacc aggatgcttg attgaaatgg cacgcatgga tctattcctc ctttgcccat
    11821 tcttggaatg ctgtattcaa ctctttgcta ccttcagaaa gtactagagt cgtacgagaa
    11881 atatccgttt cttccactag agcggcaagt acagcagcaa ctgtagagcg agtgatcgtt
    11941 tttgatgcct tctcattagg cttagttaat tgaatggaag taacttcttc atcgttagtc
    12001 aatgtgactg gttgaagaat gacaaaatct aagttagttc gtttcagcca ttcatctgca
    12061 taatgtttag taatataata atctgtcatg gactcaggcc attttgctgg ttcatctgca
    12121 taaacagcac tgaccatcag atagcgagag acatgcgcat tttcagctgc gatcactgtt
    12181 ttgattgcgc catccaagtc tacttgcaga agattctttc cttgagagcc ggcagcaaaa
    12241 atcaatacat cgatgccttt gaatgcttcg gtcatttttt ccacggacca agttaaatca
    12301 aaagagacat acgaaacatt ctcttttttt actaatgttt gggaagcaac atctctcacc
    12361 cctgcaaaaa tttcatggga acttggagct aaatctttta tcaggtgtcg gccaatttgt
    12421 ccgtttgcac ctacaacaaa aattttcatt tggatcatcc tttcgacgtc aaatctttcc
    12481 attcaatagt aacaccaaaa gagaaaaaaa tcagagatcg gctacgatga ctatcagtta
    12541 gtaagagaag ggtgcttgaa gtatcttaac aagacattgc atactgctgc agtcgcaaga
    12601 cctgcgacga ttccttcagg tataccactt gtaaaaataa ccgctagaat agctgaataa
    12661 acagcattta aatttgttcc gatgactttg gcataatctt gctggaataa aaagtagatc
    12721 atattcatca ctaaaatcgt attggtcaat gaaccggaaa gtccagctaa gaataaagaa
    12781 acggaatctg ctttttgttt cattagtttg cgcagacctt tataaacgaa ataaggaacc
    12841 accccaatca agatgcgagg aataaacgcg atcagcaagg ctttccagct tccatgttct
    12901 gttccgatga ctgggataaa cggagaaaag acaaaagaca aaggtgtttg gataactgtg
    12961 ctacggatca tgctgatcag tccaaatgta ccgccaagaa aagcacctag tcttggtcct
    13021 aaaacgatcg aagcaatgat tacaggaata tgcatggttg ttgcattgat tggaccgata
    13081 gggataaaac ctaaaggcgt aacagctaat aaaatcaaaa tagctaaaaa catcgcagtt
    13141 aatgtaaagt tttttgtatt tttcataagt actccttttt tctaagcatt tctttcatta
    13201 agagtcagca tgacctgttc gatgaccgtc tgaatatcag ctaaggcacc ttttccaaaa
    13261 tctccacaag ctaaaagaga ttctcttggt tcaatttcct gatagccgac agatctcaat
    13321 gtttccagat tacgctgagt gatcgggttt tgatacatat tcgtgttcat ggcaggggca
    13381 atcaattttg gtgttcccac tggaagcgcc attgcgactg ttgaaagaag atcgtctgca
    13441 atgccgtttg ctagcttgcc gataatgttt gctgtagcag gcgcgatgag aaacaagtca
    13501 gcttgttttg ctagttcgat atgattgatt acctgcggat ttgattcttg catgacgtct
    13561 gtatggactg cgcgcttcga cagagattgc aaggttaatg gcgtgataaa atgtgtgctg
    13621 cttttagaca taatcacatc tacctgggca cctaatttga cgaattgact tgtaagatca
    13681 gcgcttttgt aagcagaaat acttccagta acgcctagta agatcttttt cattttgatt
    13741 cctccagtct aattttctca gtaatcaatg cagcaatctc agatttactt tgagcttctt
    13801 ccactgttcc atctttagat aagagatagc cgtggtgtgt ctcgccatgg atttccgtca
    13861 aatcatttgc gaaaataaaa tcagttttgt tatttatcag actatttttt gcgactgcca
    13921 gaagagattc ctttggtaca tctactaata gtttgaaacc aatgattttt gcttttggct
    13981 gccattcacg tagataagaa atgatttttg gtgttttctt caaaacgagc acaagatgct
    14041 cggtgttaga agagatcttt gaaacagtgg tatctgtctg ttcattttcc gcaaaccaat
    14101 tgtccagttc ttcttgcgtt gaagaagtgg gtaggttttt tgccagctgt tcttctgaaa
    14161 aactaaaagc aggagtaaaa tcactgactg ccatactgtg gataatcgca tcgtagtgtt
    14221 cttttttcgt tagttcttct aattgtaaaa ataagtccag tgcagattcg atcgtataaa
    14281 tcgttatatt ggagtgtctt ttcggtttca gtgcttgttt cgtagtcacg taatcgattg
    14341 tggtatccgg attggcagct aaataatcag ctaatgcttg ccctaatcgt cctgtcgaat
    14401 gatttgtaat agcacgtact tgatcgattt tttcagtcgt tcctccagct gtgattagaa
    14461 tcttcattta tggtcaccta catttactag tgttcttcct tggtgtgttc cagcaagtag
    14521 tttagatgct gtttcaggca attgatccag agtaatctct tggacgacca gttcatctgc
    14581 aatctgcaaa tccgccatct gttgccataa agaaagacgt tgatctattg gtacattgac
    14641 agaatcaatt ccaatcaact ggatgtttcg cagaataaaa ggcaagacag tcgcgtttag
    14701 tttgattccc ccagcattgc cacataaagc tactgcaccg ttataagaga tcaatggaag
    14761 aagactgctt agctgctcgc cacctactgt gtcgatgaca tagtcgattt gctgttttcc
    14821 taaagctttt gttgtctctg gcagaaattc atcaggtcgt acgacttgca aagcacctag
    14881 actttttagc catgtgacag actcttcttt tctggaaaag gcaatgattg actggaatcc
    14941 tagtttatgt aacagggcta tcgctgtact gcctacaccg ccagaagcac cagtaacgac
    15001 aactttttta tcggaggtca taccttgacg gattaaagcg ttcacacaca acatagcagt
    15061 aaaccctgct gtaccaagga tcatcgcttg acgcagcgtc atattttttg gtagaggaac
    15121 taaccagtct cctggtactt tttgatattg gctataaccg cctggatggg tgacgccaag
    15181 cccaaaacca gtagcaagta caagatcttc ttttgaaaaa cgtggattgt tactttggac
    15241 gacagttccg gataaatcaa tccctggagt catcggatat gttcgtatca cctgaccaga
    15301 ctcgctcatt gccaatgcat ctttataatt gacatcagaa taggcaattt ttaccaagac
    15361 ttcattttca gccaactcag taaatggcag atcactgatt gctgtatgga aaacaggttc
    15421 ctttattacc tgaaaagatc gaaaagcgtt cattcgtgag acactccttt taaaattttc
    15481 ttttatcata ccataaagcc tactctttta ttcttgttct tccgtaaatt tagacaaaaa
    15541 ttaggtgaaa aataaagaat caaacaacta gaatttattc caaaagataa agaaatattc
    15601 agcaaaaaaa attttacaaa gggttgcctt tttcaataaa tttcgtatac taatacgagt
    15661 ggtagtaaac aggaagttta ctattagaaa acttcgattc ggaggaaatt atggaaattg
    15721 gtgagaagtt aagaaatttg cgcatccaaa agaatttgac acaagaagaa ctaggcgaac
    15781 gcacagattt gtcaaaaggg tatatttccc agttggaacg agatttaagt tcaccatcaa
    15841 tggaaacatt cttttctatt cttgaagtct taggtgtgac tccagaagaa ttttttcatc
    15901 aagaagcagc gaatctgcaa gtggtttatt caaaagaaga tcatacggtt tatttcgatg
    15961 aagaaaacgg gtatgaattg gaatggctcg tggctgattc gaatgaaaaa gaaatggagc
    16021 ctgtcttgct gacttttgaa ggtgcaggag aatataagac gtttgaacct tcattatctg
    16081 aaacgtttat ttttgtatta gaaggggaat tagagttaag cttaggagag acggtctatg
    16141 tggcaaaagc aggacagtcg atctattatc aagcgacaaa acaacatcaa ttaaggaatc
    16201 atctcaaaaa acgaacaaaa gtattgattg ttgcaacgca atcatatttg taatataggg
    16261 ggtaaaaacg tggggaaacc aatcatttca tttgatcaag tagtgaaacg ctatgatgat
    16321 gaaacagtat taaaaaaagt aagttttgag atcgaacaag gaaaatttta tacactgctt
    16381 ggtccttccg gctgtggtaa aacaacgatc ctacgtatta tagctggatt caccgaagct
    16441 acagaagggg acatctattt tgaagggaaa cggttgaatg atttgccagc taataagcga
    16501 caagtaaata cagtcttcca agattatgca ctttttcctc atatgaatgt ttttgataac
    16561 gttgcttttg gattgaagat caagaaaatg tccaaatcag atatcgaaaa gaaggtaaaa
    16621 gatgctttgc gtatggtcca gcttcctgga tacgaaaaac gggagattag tgaaatgtct
    16681 ggtggtcaaa gacagcgtgt agcgattgca cgagcaattg taaatgaacc gaaagtattg
    16741 ctattagatg aaccgttatc ggcgcttgat ttgaaactac gtacagatat gcagtatgaa
    16801 ctgcgggatc tgcaacaacg attaggaatc acgtttattt ttgttactca tgaccaagaa
    16861 gaagcgttgg caatgagtga tgagattttt gtcatgaata aagggaaaat cgtccaaagt
    16921 ggtacacccg tagatattta tgatgagcca atcaatcatt tcgttgctga ttttgttggg
    16981 gagagcaata tcgtggacgg tgtaatgatc gaagataatc tagttgagtt tgttggtaag
    17041 caatttgaat gtgtcgatgg cgggatgcgt ctaaatgaac cagttgaagt tgttttacgc
    17101 ccagaggatt tgacgatcac gacaccagat aaaggaaaac tagtcgtaac ggtcgatact
    17161 cagctgtttc gtggtgtcca ttatgaaatc atctgttatg atgaacagca aaatgaatgg
    17221 atggtccatt caacgaaaaa agctaaagaa ggaagcaaag taggattggc ctttgagcca
    17281 gaagatatcc atgtcatgcg tttcaacgag tctgaagaag aattcgatgc acgtcttgac
    17341 agctacgaag aatagggggg acaagaatga aaacaatgcg caggatctat tccgtccctt
    17401 atattttgtg gctcggttta ttcgtgatcg cgcctgtatt aatgatcatt tatcaatctt
    17461 tttttgatat gaatgggcaa ttcacgctga ataattatct atcttatttt acgtcgggga
    17521 catcgctttc tatgacgatc aattctgtat ggtatgcatt tttgatcacg tttttcacgc
    17581 tgatcatcag ttacccgact gcttattttt taacaaaact gaagcacaag caattatggc
    17641 tgatgctgat tattttgcct acatgggtga atctgctgct taaagcttac gcatttattg
    17701 ggattttcag tgtgcacggc agcgtgaatc atttctttga ttttatcggg atcgggccga
    17761 aacagatatt attcacagac ttcagttttc tgttcgtggc aacgtatatc gagattccat
    17821 ttatgattat gccaattttc aatgcattgg aagagatgaa tccttctttg atcagtgcta
    17881 gtagagattt aggcgcgaat agctttgaga ccttccgacg agtgattttt ccgttatcat
    17941 taaatggtgt gaaaagtggt gtccaagcag tatttattcc ttctctttct ttattcatgc
    18001 tgactcgtct gattggcgga aatcgggtga tcactttagg aacggctatc gaacagcatt
    18061 tcttggttac acaaaactgg gggatgggtt ctacaatcgg tgtgatttta atcattgcta
    18121 tgttcatcgt gatgctcctg acaggcgaga agaaaaaagg aggtcgctca taaatgaaaa
    18181 aatttaaatg gtcgtatctt tatttaggga tcgtcttttt attcttatat gcaccgatct
    18241 tctatctgat tttttattcc ttcaatgaag gaggaacgat gaacagcttt acaggattta
    18301 cttgggaaaa ttatgaagct gtttttgaag atacacgttt gatcattatc gtattgaata
    18361 cgttcatgct ggctttctta tctgcattga tcgccacgat catcgggaca tttggggcta
    18421 tgggaatcta ttacacgaaa aaacgacgtg cacggacagc gttgttgagc atgaataaca
    18481 tcttattagt ctctcctgat gttattatcg gtgctagttt cttgattttc tttacactac
    18541 taggattcgg attaggtttt cagagtgtat tgctttccca tattgctttc agcattccaa
    18601 tcgtcgtctt gatggtcttg ccaaaattgc aggaaatgaa cgattcaatg gtcgatgcgg
    18661 ctagagacct tggtgctaat aatttccaag tagtcaaaaa tatcattttg ccttatcttt
    18721 ctccaggaat catagctgga tattttatgg catttaccta ttctcttgat gattttgcgg
    18781 ttacattttt cgtcacagga aatggcttta cgacgctctc agtagagatc tattctcgtg
    18841 caagacaagg aatcagttta gaaatcaatg cgctaagtgc gttagttttc ttgttctcca
    18901 tgatattggt cattggatac tactttatta gtaaagaaaa ccgtccaaaa cggctgaaga
    18961 aaaacagaag agtacgcgag ggggtggcac ggctgcgatg aaaaaattac aatctctgtt
    19021 tttaggtatt ctcatcatta ttgcggtgct gctgcttggt gttcgtcaac ttgaaaaaac
    19081 aagtggcatg agtggagcaa aggtacttac catctataac tggggagatt atatcgatcc
    19141 tagtttgatc acgaaatttg aaaaagaata tggatacaaa gtgaattatg aaacatttga
    19201 ttctaatgaa gcgatgttca caaaaatcca gcaaggtggg acaaactacg acatcgctat
    19261 accaagtgaa tatatgatcc aaaaaatgat caaagaaaaa ctagtacttc cattagatca
    19321 ttcgaagatc aaaggattaa ataatattga tccacgattt ttagacttgg attttgatcc
    19381 agataatcag tattcgattc catatttttg gggaacttta gggatcgtat ataacgataa
    19441 attctttgat gcaaatcaaa ttaagcattg ggatgatttg tggaagccgg aattaaaaga
    19501 cagtttgatg ctgattgacg gtgcacgaga agttatgggg ctttctttga atagtttagg
    19561 ctattctttg aatagtaaga atatggcaca gctgacagaa gcagcaaata aattgaatca
    19621 tttaacacca aatgtcaaag caatcgtggc agatgagatc aaaatgtata tgatcaatga
    19681 agaagcctcc gtggcagtga cattttctgg cgaagctgcc gatatgatgt gggaaaatga
    19741 acatttacat tatgtgatcc caccagaagg atccaatctt tggtttgata atatcgttat
    19801 gccaaaaacc gtgaaaaata aagaaggtgc ctatgatttc atcaacttta tgctagaacc
    19861 ggaaaatgcg gcacaaaatg cagagtatat tggttattct acaccaaatg aagaagcaaa
    19921 agcgttattg ccaaaagaca tttcgtcgga cgaacaattc tatccatctg atgacacgat
    19981 ctcccatctt gaagtctaca aagatctagg atcgaaatac ctaggcatct ataatgacct
    20041 attcttagaa ttcaagatgt ataggagata atggcaaaac tcagtaagaa tggggactta
    20101 tcaacgttct aaagagctaa aaatcgtcct ttttctatct gagttttcca atcttctacc
    20161 aaaagcggat gaaacgccgc tttttttgtg ttattttatt ttttctacca cgtttttcta
    20221 ccagagaaaa gtggacaaaa ccattgtaaa atagggcccg agacgggcta gtaaatctaa
    20281 gattgaaaaa gttacagaag gataaagtaa ttggaaacta tgcaaatata cttaaccctt
    20341 ctgtaatcta ttaataaaac taatatatga atccagttca atatttgtag ctatgaaaaa
    20401 acataaaaat catatta
//
LOCUS       DACKAC010000049        19639 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.049, whole genome shotgun sequence.
ACCESSION   DACKAC010000049 DACKAC010000000
VERSION     DACKAC010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.049"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            283..1368
                     /locus_tag="I0B46_001891"
     CDS             283..1368
                     /locus_tag="I0B46_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAR2084173.1"
                     /translation="MSTEFQSQKMIIKNWGYKATNVYIEKYYKFPSFLIQNSKYSKMK
                     DSSKIAYMLFKNEFRRALHEKWIDDDGLLYLEFTQADLMDKLNCYQGKVKTILNELET
                     YNLISVVKGEFNPKTKKNEKNKYYLLQPEISADDLFIQNSFDDDLSETIDKSTSSRNA
                     EIARREETAKTLDNTGNAEIARREKTAETLDNTGNAEIAQDYYINKSLDTKRHLKDTE
                     TDQLQNQVLLDNFVEIMKEDSINTFVPEKVLSLIKTFSNTYEEAQQTVTTIHNAKHKA
                     EELEKTVVVYEELESYGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEK
                     AIAAKLANEQRETFNELPTVSTENWLE"
     gene            2204..2536
                     /locus_tag="I0B46_001892"
     CDS             2204..2536
                     /locus_tag="I0B46_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAR2084174.1"
                     /translation="MSYTGILSFEDICHYGKRCTATEKITKKLSTGQNKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            3127..3456
                     /locus_tag="I0B46_001893"
     CDS             3127..3456
                     /locus_tag="I0B46_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084175.1"
                     /translation="MIQKFAKMSKTAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVDDKCPVKISFSDIATIIEGIYYGEVDEEISSTKDLLDFLDKNKDTFIVLTDYEE
                     VTTNEKL"
     gene            3443..3784
                     /locus_tag="I0B46_001894"
     CDS             3443..3784
                     /locus_tag="I0B46_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAR2084176.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQKLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            3898..4137
                     /locus_tag="I0B46_001895"
     CDS             3898..4137
                     /locus_tag="I0B46_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084177.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKDVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            4204..4443
                     /locus_tag="I0B46_001896"
     CDS             4204..4443
                     /locus_tag="I0B46_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084178.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVMQYINDMQIT
                     GKEKACLLGSMKNFEQLRRTYVKKSSNCQLLLEVS"
     gene            4529..5200
                     /locus_tag="I0B46_001897"
     CDS             4529..5200
                     /locus_tag="I0B46_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAR2084179.1"
                     /translation="MRKWLFGFLGVALIVFCSVFGYVSYQKHEGKVFKQNIEKKMPVD
                     QINEHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQAFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            5253..7229
                     /locus_tag="I0B46_001898"
     CDS             5253..7229
                     /locus_tag="I0B46_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAR2084180.1"
                     /translation="MAINRYVKKAGMLVGMLGIIASCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDKEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVINGTGAEHSFQIGDAVNYQGTIHIPTDIAGGTDGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDIQLTYNMFVNDSAAIANPLTNSVDFVYSNNPFNQEEHHEKTKADAVTYGAKFLKA
                     DGGLFGTGIKATPLEGAEFAVKNAEGKYYGGLVDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGEYSLEETKAPTGYQKLTEDIAFKVNKNSFKEENRITVKNNQKAAI
                     PLTGSNGFQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            7242..7985
                     /locus_tag="I0B46_001899"
     CDS             7242..7985
                     /locus_tag="I0B46_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAR2084181.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPSVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIEEVQ
                     KDELQRNFVTYLEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEENRKQ
                     KLSWKRLLGSTVFLSVMIIGSLFVYKKKK"
     gene            8001..8774
                     /locus_tag="I0B46_001900"
     CDS             8001..8774
                     /locus_tag="I0B46_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084182.1"
                     /translation="MRNRKWLLVCAVALFSFTAYTQLTFADSQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGQKLEKQEGV
                     NGAKFIVYDITNILEEMSQDDNEKNQSVEIIESKLKDRAKKLSYDQLKKVAEGETKTI
                     DDQEGVIEFSIEVPANKKQAYYIVNESSPENISNSEDIVLLTPVSNEKGEFLTDVWLY
                     PKSEKSEPKEEIKKVVSTGVKKNFFENCWEFFAQLFFDN"
     gene            9063..11174
                     /locus_tag="I0B46_001901"
     CDS             9063..11174
                     /locus_tag="I0B46_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAR2084183.1"
                     /translation="MEGIMENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQ
                     IKENNNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAH
                     YQKVGTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQ
                     SINVMNAKLEFKYAGTKTKVALSEPQPEIQSKMRSMRAAGIDLNDTFLSIYSLNGQYQ
                     QRVSWYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSK
                     VVGGRAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTAL
                     LWTPQPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKS
                     FKITDTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSF
                     LANAANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNTVENYFSLKPN
                     KKVEQDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQFSIQD
                     PVEDRLTYKEGSARVIDMSTGKDITSEGTLTYDSNSRTLKWEASADFLSKNLLDGREI
                     QLIFTAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNHLVLT
                     VIGSILFLTLASLGYVVLKLK"
     gene            complement(11212..13269)
                     /locus_tag="I0B46_001902"
     CDS             complement(11212..13269)
                     /locus_tag="I0B46_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAR2084184.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNHEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKASQAQVNNAKNSLDAAFKGLQHKPETPKEYTVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVNILKEQIVNVTHGDSVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFATPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(13383..13595)
                     /locus_tag="I0B46_001903"
     CDS             complement(13383..13595)
                     /locus_tag="I0B46_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084185.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            13782..14249
                     /locus_tag="I0B46_001904"
     CDS             13782..14249
                     /locus_tag="I0B46_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084186.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIRKFI
                     NKPDSLFIVVPYEKDGVKKIVFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            14246..14488
                     /locus_tag="I0B46_001905"
     CDS             14246..14488
                     /locus_tag="I0B46_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084187.1"
                     /translation="MIASLFIYPKGGIIVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            14506..14808
                     /locus_tag="I0B46_001906"
     CDS             14506..14808
                     /locus_tag="I0B46_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084188.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            14880..16934
                     /locus_tag="I0B46_001907"
     CDS             14880..16934
                     /locus_tag="I0B46_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084189.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKDTDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETDEVEVDPVEA
                     NEESTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKVKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLEIRKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSESNE
                     VK"
     gene            16934..19546
                     /locus_tag="I0B46_001908"
     CDS             16934..19546
                     /locus_tag="I0B46_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAR2084190.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFCEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSLENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
BASE COUNT         7103 a         2958 c         3862 g         5716 t
ORIGIN      
        1 tttattacga atattgtctt ctcaataaaa agttcttgat tataagatct ttcaatcaga
       61 tttatttttg aataaggtgt cttccaatca aaagtaataa ctgatttaag tatctcttat
      121 tcaaaatata gttgtctgaa aataataaac tgttaaactt attttgaagc ctatattatt
      181 cctgatttgt atatcgaaat tcattagcta tatacattta aagaaatcta attatatttt
      241 tgataataaa aattgtacgg atactagaaa agagggagtt taatgtctac agaatttcaa
      301 tctcaaaaaa tgattattaa aaattgggga tataaagcaa caaatgttta tattgaaaaa
      361 tattataaat ttcctagttt tttaattcag aattctaaat attcaaaaat gaaagacagc
      421 tcaaaaatag cttatatgct ttttaaaaat gaatttcgta gagctttgca tgaaaaatgg
      481 atcgacgatg atggactatt atatttggaa tttacacaag ccgatctaat ggataaatta
      541 aattgctatc aaggcaaagt aaaaaccatt ttaaacgagt tagaaacata taatttgatt
      601 tctgtcgtaa aaggagaatt taacccaaaa acgaaaaaaa atgaaaaaaa taaatactat
      661 ttacttcaac ctgaaataag tgccgatgat ctttttattc aaaattcttt cgatgacgat
      721 ttaagcgaaa ctattgataa atcaacctct tctcggaatg cggaaatcgc acgtcgagag
      781 gaaactgcta aaacccttga taatactgga aatgcggaaa tcgcacgtcg agaaaaaaca
      841 gctgaaaccc ttgataacac tggaaatgcg gaaatcgcac aagattatta tattaataaa
      901 tctttagaca ctaaaagaca cttaaaagac actgaaacag accaattaca aaatcaagtt
      961 ttgctagata acttcgttga aatcatgaaa gaagacagca taaacacatt tgtaccagaa
     1021 aaggtattaa gcttgataaa aacattttct aacacttacg aagaagccca acaaactgtc
     1081 accacaatac acaacgcaaa acataaagcc gaggaacttg agaagacggt agttgtgtat
     1141 gaagaacttg aatcttatgg aattgatgct gacaaaggat tatatatgac tttactgaaa
     1201 gcttatcaaa aacaaaaaac tgaaaaagta aacaatttgc agaatctaat tttcgtttat
     1261 gtaaaaaatt ggtttgtcga aaaagctatt gcggcaaagc ttgcaaacga acaaagagaa
     1321 acatttaacg aattaccaac agttagtacg gaaaattggt tagagtagtt gataaaaagc
     1381 gatcactata aaaatgatca attttaaatt agaacttttt tatgatttta attttattac
     1441 aaatgttaca aataaatagt tattattccg gagtttttag attaaaagtt tatgaaaaca
     1501 atcacaaacg taggttttaa agtcataatc gttagatatt ttagttattt ttcattcaaa
     1561 aaaataggga actagtgttt gcatatgtgc ttttttgagt tataataatt catgaagaga
     1621 ggtaattcac ttatttcccc aagctgaaaa gcctgatatt acttcttttc aggcagtgct
     1681 gctaatattc aattacactt ttttgtatat ttttagttgt tttaaattta attgtctgat
     1741 atgcttgtga tgaattgatt ttcgttctgc acttttggac gataaattgt gataattgaa
     1801 aatacaaaaa aaagaaaggc ccctgtaagg ctcgggagac tggtaatctc cgtcagtgtc
     1861 ctacaggaaa gcattaagat gacgccttaa tgcgtgccct tcagtgttac ggtattaccc
     1921 gcaacggact cagtataaca gatattcaaa agtgatacca ctttttttgg ctctattaag
     1981 gttaaaaata cgtgatattg taatgaatct atgcctaagc ataatgttat aaaaatgtcc
     2041 gtgtggctct ttgccgtatg acgaacgctg aaattagagg gaacgcatgt ttgaatgcgt
     2101 tccctctttt ttgtttgtca tgaacgaaaa tcaattctct atttttgaaa aataacaaga
     2161 aaaacttatt aaaaatattt aaagaaagtg agaggattac tttatgtcat atacaggaat
     2221 tttgagtttc gaagatattt gtcattacgg taaacggtgt accgctacag agaaaatcac
     2281 gaagaaacta tcaactggcc aaaataaaac cgttgtgcag tgtaagaaat atataatcca
     2341 gaaagacaaa gtctcagaag aaatgattta ttacattggg aaacaaaagc aaataatttt
     2401 aaaggatcct atccctttaa aagagctata tccaaccatc aaacatgttt atgaccaaaa
     2461 cggtgtactg attggtcgaa gaaaaaatgg agtcttgcgt tgtaccgcaa aagggatggg
     2521 gcgtttgatc agctgaattg atcattctca taacttgtat tttatcgttt ttcgcttgct
     2581 tgaaaatatt gtcgagactt ctaaaggaac ttagaatcat tagaaaaata aggagtagac
     2641 ttgatctatc aacaattccc ttcggggagt gacggggtag gtttttacga ttggtaacgt
     2701 aaaaaattgg tcaatcgagt aaatgccctt gtttggatag tggtgaaatg aatttgtgta
     2761 aataatatgc acagagtaaa caagagggaa tttagaagtt agacaagtta gaatagcgcc
     2821 tagattaatc atgagcagct actaagggat ctaggctttg acgcaaactc atggaataag
     2881 gagtaggtct tctcgtaatg gggaggacgc acaagaaatt gtgtcgtgtg ctgcagtaca
     2941 tgtagatact tgcgaggacg ttgattattg gatcaatcga gcatttaccg ctgtctacta
     3001 gggtttctcg tttcggacgc cataaacgag tcgatttatt ttatatattt ttgagtgaac
     3061 gaaaaacgat aaagatattc gttatgaagt aaaactaatt tagtagatag caaaggtggt
     3121 agaggaatga ttcaaaaatt tgcgaaaatg agtaaaactg catggattga aaagcttgaa
     3181 aatgacttga gtgtcatgcg attagctcca aagaaattca aacgagagat tgcagaaaaa
     3241 gaagcaattt tgtcggtatt gaatgcggta gacgataaat gtccagtaaa aattagtttt
     3301 tcagatatag caacaattat cgaaggtatt tattatggtg aagttgatga agagatatct
     3361 tctacaaaag atttattaga ttttttggat aaaaacaaag atacctttat agtactaact
     3421 gattatgagg aggttacaac aaatgaaaaa ttatgatccg aatatacgat tgggcacaca
     3481 tacgattaag gtttctttcc aacgttggga ttacaaagga tttgtgacgt ttagaagagg
     3541 cggaaattgt aaaggattag atgttttagc attagacgag gatgatttat acgatcaaaa
     3601 attgactgat aatccaattg gattcggctt gctacctgaa gatgatgaag gtaatgaatg
     3661 gtttaaaatg acgcttatga atgataatgg agacgaatta tctgttgaag atacttggtc
     3721 atatttgagt gattatattg tcagcgttga aatcattgaa tttgtagcgg ataaagaaga
     3781 ataaaaactg gaggaataaa ttttacagat aaaggcttag aaggagtcac gacgattaat
     3841 aaaaaggtag tattacctta ggcattgagt aaggaattta tttaggagga aaattagatg
     3901 gcgcgaccag ttttagaggc gatggatatg gatactttga caaaaggaag gtatgtacag
     3961 ctgttagctc gtggttattc acctaaagat gtattcaaat ccctgtctaa aggaacagat
     4021 atggaaaaag aaagattacg aaaagaattc gattattgga gaacccataa tggaatcaag
     4081 gatttgaaac cagcacggcc agtaattaaa cgaaagaaaa agataaccca agactgaagt
     4141 gtagagcgta tgtgatcata ggctttttta tttttgcaaa aaacaaggaa ggtaggaaaa
     4201 ctcatgaatt atgaagaaat cgaaaacaga aaaaaagtgt cgaaagaaat ggaagaaaag
     4261 ttgctaaaaa cgatgaaaca aaaacacctt aaaagattat ctgtcatgca atatatcaat
     4321 gatatgcaga taacaggaaa agagaaagct tgtttgttag gttcaatgaa aaactttgaa
     4381 cagttgagac gaacttatgt gaaaaaaagt tctaattgtc aactattgtt agaagtctca
     4441 tgacattcat taatagaatt catttttaaa actttttcta tggaaatgag aaaaagattt
     4501 tttgttttgt ctgaaagggg gtagctggat gagaaaatgg ttatttggtt ttttaggagt
     4561 cgctttaatc gtcttttgca gtgtgtttgg ctatgttagc taccaaaaac atgaaggaaa
     4621 ggtgtttaag caaaatatag aaaaaaagat gcccgtagat caaatcaatg aacatgcaaa
     4681 gagttataag gaggatgcaa cgaacgtcaa taatgatatg tctttggggc aaatgttgtc
     4741 catacaaaaa gaagcaatcg aaatgggtgt caataaacaa gcctttgcac aaattcagat
     4801 acctgcactt ggtttagcat tgccaatttt taaaggggca aatcaataca ctttgagtct
     4861 tggagcggct acttattttt atgaagatgc cgaaatgggc aaaggaaact atgtcctagc
     4921 aggtcataac atggaaatgc caggtgtttt attttctgat attcaaaagt tgtcattagg
     4981 ggaggtgatg gacttagtta gtaacgatgg cgtctatcgt tacaaggtaa cgcgtaaatt
     5041 tatcgtacct gagtacttta aattaatcga tggcgttcct gaagagaact ctttcttgtc
     5101 tttaccaaag aaaggagaga aacccttatt aacgttgttt acttgtgttt atacgtcaca
     5161 aggaaaagaa agatacgtgg tacagggaga gctacaataa caaggtttta atcaacgcga
     5221 gtcgttgaaa tatgaaatga ggagagtaaa aaatggcaat aaatcggtat gtaaaaaaag
     5281 ctggaatgct tgttggaatg ttaggaatca ttgcaagctg ctttcagggg tatcaagtac
     5341 acgcagaaga tgtcacgcaa aaaacgccac cagagaaggt caatatcacg gttcataaat
     5401 taatgtacga tcaaggaacg caattgaatg ttgatattga cgggatcaaa aacgatggtt
     5461 atacgcacga tcaatatcct acaggtgtaa cgaaatacaa caaggcagat tatggtgatg
     5521 ttgagtttac gttaggaaat attacggatc aagtattacc gagggaagat tctgatctaa
     5581 ctaacgcgaa agtggacgaa atcgtgaaag acgtagaaga caagggatct aacagtgagt
     5641 acgtcaagaa tgcaaccaac aaaattactt cagctgtaga tgaaaatggt gagatcactt
     5701 ttgctgatca accagcctat gtcaattcca aagggaatgt atatgtggtt tatgaaagta
     5761 aatccgctgc tggattagtc acacaaaaag caaaaccaat ggttgtgatt gcgccaatga
     5821 ctgataatac aggttcaggt tttttaaaag acattcactt atatcctaaa aatattgttt
     5881 ctaaattgag ttttgaatta acgaaatttg gtgacgatgg cacagctcaa agcaaacaaa
     5941 cacctttaaa aggggcaaaa tttgagttat acaagggaga acctggaaaa ggaacaaaat
     6001 taggagatct tgtatcggat gatcaaggaa agctgactgc tactgatttg acgttaggaa
     6061 aatactattt tgtggaagtt ccttcagaag ttgtagtggg atcagataaa gaaccaacag
     6121 cagatcaata tttattaggc gcagacgctc gcaacgatgc acacaataaa ttaaccttcg
     6181 aaattacgaa tgatggcgtg acaagtgact taaaagcttc ctatgtaaat tacaaagctc
     6241 ctgtaatcga taagacagta atcaatggaa caggggccga acattctttc caaattggtg
     6301 acgcagtgaa ttatcaagga acgattcata tccctacaga tattgcaggt ggaacagatg
     6361 ggattacagt aaatggtgtg aagagtgaaa cttctcctta cagcgtattt aagtggggag
     6421 atacagcagg taaaggactt tcttatgtcg ctgctaaagc aaatatcaaa gtaacaaata
     6481 aagatggatc agtggtatta aaagaaaata cagattataa gatccaaaat tcagaaaatg
     6541 gctttgtgat cgattttatc gtgaataatg gccaagtaag tgatactgtc gcaagtttac
     6601 acggacaaga tattcaactg acttataaca tgtttgtgaa tgatagtgct gcaatcgcaa
     6661 atccattaac aaatagcgtg gatttcgttt atagcaacaa tccattcaat caggaagaac
     6721 accatgaaaa aacgaaagcc gacgctgtga cttatggcgc aaaattctta aaagctgatg
     6781 gcggactatt tggaacagga atcaaagcaa caccgcttga aggcgcagaa tttgctgtta
     6841 aaaacgcaga aggaaaatat tatggtggat tagttgatac ggataaagac ggtgtcaaag
     6901 aagcagtatg ggtagacgat gtagcaaatg cagcgatcct aaaatcagat aaagaaggtc
     6961 actttgaaat cactggttta actgaaggtg aatattcact ggaagaaaca aaagcaccaa
     7021 ctggttacca aaaattaaca gaagatattg catttaaagt aaacaaaaac tcatttaaag
     7081 aggaaaatcg aatcactgtt aaaaataatc aaaaagcagc tattccatta acaggtagta
     7141 atggtttcca aacgtatgta ttgatctctt gcttgttgct aggagcaggg gcactttctg
     7201 cagttgttta ctttaagaag aaagcctaga aggatcagct aatgattatt cgtaagcccc
     7261 caaaaagaag agtcatgcac aaactgatga tatttttttg gttgctgtcg gcagtagcag
     7321 tctatttaat tccatctgtg gaaacaaatg tagcgaaatt tcaacaacag caaagaattt
     7381 tagccattga aaatcttgaa aaaacagatt cttctttgga tgttgcgaaa atagagttag
     7441 gcgatcctgt tggtgttctt acaattccaa gtatttcttt aaaactacca atttatgacg
     7501 gaacgtccga caaaatttta gaaaatggtg taggtattac ggaaggaact ggtgatatta
     7561 caggaggaaa cggtaaaaat cctttaatcg caggtcatag tggactttat aaagataatc
     7621 tttttgatga tttaccttca gtaaaaaaag gagaaaagtt ttatatcaaa gttgatggtg
     7681 agcaacacgc ttatcaaatc gatcggattg aggaagtgca aaaagacgaa ctacaaagaa
     7741 actttgtcac ttacttagaa ccaaatccca atgaagatcg agtaacgcta atgacatgta
     7801 cgcctaaagg aatcaacaca caccgtttcc tagtgtatgg gaaacgagta acctttacaa
     7861 aatcagaatt aaaagatgaa gaaaatagga aacaaaaatt atcttggaaa cggcttttag
     7921 gaagtactgt ttttctttca gtcatgatca ttggttcgct atttgtttat aaaaagaaaa
     7981 aataaaggag ggtaaccaac atgagaaata gaaaatggct acttgtttgt gcagtcgctc
     8041 ttttttcttt cactgcctac acacagttaa cgtttgcaga cagtcaagaa agcacggata
     8101 gcacagaaca aacaatccaa acgtcagata gcagcacgat tcaaagcagc gatgaaaccg
     8161 tcgattcgtc aacagtggat tcttcagaag aaccgaaaat tgctgaagac gatcaaatag
     8221 tcaaacagac cattcacgtt caaaagatta tttcttcaga acaattagac aaccaggggc
     8281 aaaagcttga aaaacaagaa ggagtaaatg gcgcgaagtt tattgtctac gatatcacga
     8341 atatcttaga agaaatgtca caagatgata atgagaaaaa tcaatctgta gaaataattg
     8401 agtcaaaatt gaaagatcga gcaaaaaaac tttcatacga ccagttgaaa aaagtggctg
     8461 aaggagaaac caaaacgatt gatgatcagg aaggcgtgat tgaattttcc attgaagtac
     8521 ctgcaaacaa aaaacaagca tactatatcg ttaatgaatc aagccctgaa aacatttcaa
     8581 acagtgaaga tattgtttta ttgacacctg tttcaaatga aaaaggtgaa tttttgacgg
     8641 atgtttggtt ataccctaaa tcagagaaat cagagccgaa agaagaaatt aaaaaagttg
     8701 tgtcaactgg tgtaaagaag aacttttttg aaaattgttg ggaatttttt gctcaattat
     8761 tttttgataa ttaggatgaa atgatgaaaa taataagaat attagttttt aatctatcct
     8821 ttttagccgc aacaatattg tgttgcggtt taattttttc tcaattatca atttatccaa
     8881 gatatcaacg attgttcatt cattttctaa acattcaatg tgcttccatg ccattagagg
     8941 tgattaaaaa gctcttgatc aatccaattt ttatggttag tttggttctt cttacaggat
     9001 tggtaacgtg ggtattagga aaaaaagaat gaaaagctta cgatcgttta aaaaaatatt
     9061 agatggaggg cattatggaa aataaaattc attgcactac gggaagagta attactttag
     9121 tgttgttttt gttccttctt ctttttgcga ggggaactgc agtccaggcc ttagaaaaag
     9181 ttgatccgag tcaaatcaaa gaaaataata atctggtgct ttatcatgat agtgattcat
     9241 tgagaaatat cgaagtaact gggatcaatg gatttaagat actagatgag tattcaacta
     9301 aaaaacaaaa agtacttgct tggccaaaag tcaaagattt gaaaagtacg tcaaaaatcg
     9361 ttgcccatta tcaaaaagta ggtacttatc taggaaaaga aattgattgt gacgtgactt
     9421 tttctaattt tgtagcgcac gctggggtta accaagacgg attagctaaa acaattaatg
     9481 gcaaaaagta ttataattat ttgctctttg ataaaaatat ttatcgagga tttttcatgc
     9541 agtcaatcaa tgtcatgaat gcaaaactag aatttaagta tgcaggaaca aaaacaaaag
     9601 tagctttgag tgaaccacag ccagaaattc aatcaaaaat gagatcgatg agagcagctg
     9661 gtattgattt aaacgataca tttctatcca tttacagttt aaatggacag tatcagcaac
     9721 gtgtgtcttg gtataatgac aataatgaat ctgtcggtga acgtaatatt gatatgagag
     9781 aatttgttgg gtatgaaaaa atgggtagct taccttattt tgtcacaaca gatacagcat
     9841 gtgcagaata caaagctcct gcgttatcaa caaacaattt aacttcaaaa gtagtgggag
     9901 gacgtgcaga aaaggcttat agctcgaatg atcatttcac cgatgttgta ggagctgata
     9961 cttatcacag aagtggtgta acgtatacgc ttcaaggcgc ttccccaaca ttcatgattg
    10021 gcgcaaatac gaatagtatg atgtttagct ttgatactgc attgctatgg acaccacaac
    10081 catcgaagcc tacaaaagaa gtgtttaaca aagctaatac tgaagaggca gcacacaata
    10141 ttgacaaaaa agtgattcca caaggatcag atgtttacta tcatattcac caaaagtttg
    10201 atgcattaac agtcaacaca atgaacaaat acaaatcatt taaaatcact gatacctttg
    10261 acagcaaaaa ttttgatatg gtatcggatg ggaaaaacta tgatggcgca ttgtggtatg
    10321 ttgaagcgaa tggtacttgg aaaaaagctg atacaggagg aaagttaact gtatcaggta
    10381 atacagtcac ttatactgct tctgacagct ttttagctaa cgctgcaaat tacggtaggg
    10441 attatatttt gcagctccat atgagagcaa aaaatgatgc aaaaggaatt gcaccaaatg
    10501 tggctactgt aacgtttaac aatgatccga acaaaggaac acaaaaaaca aatacagtag
    10561 aaaactattt ctctcttaaa ccaaataaaa aagtggaaca agatgggaaa gatgttgatg
    10621 gtcgaaatga tggtaaaaaa ggaacgccta cagctccttt aaatgcagga tctgaagtac
    10681 aatatctagt cacacaaaaa tggcatacaa aaggtgtaga cattgcctca gatcattaca
    10741 agcaatttag tattcaagat ccagtggaag atcgtttgac gtataaagaa ggttctgcgc
    10801 gagtgataga tatgagtaca ggaaaagata ttacttcaga aggtacacta acctatgata
    10861 gcaattcaag aacgctgaag tgggaagctt ccgctgattt cttaagtaaa aatcttttag
    10921 atggacgaga aattcaactg atatttacag ctaaaactcc tttgaatgaa gaattggata
    10981 tcgacaacca agcaaaagtt tcagtagaaa gtattgagaa agcaactaat gtagtaacga
    11041 ttggggtaaa acctaactta cctcagatca ttgtgccaaa aactggatcg aatcatttag
    11101 tgctaactgt tattggaagt atattatttc ttactttagc atcattgggc tatgtagttt
    11161 taaaactcaa ataaatataa aaacaaggct gagaatcagc cttgttttct tttatttgaa
    11221 gattggattc gcactaataa atacattatc tacgccaatt ctaccagcag agtcaactac
    11281 aaaattatga cctaatccga gaatactttg gttattataa agtaaatttg cattatgggc
    11341 agggctattt ttccatccta ccatggcatc gatagcacca tcgtctgata aaaggtcatc
    11401 aatatacagc ccagtttgac ttatgttttc tcccttagca ccgtagcagc ctaagctttc
    11461 caataaatct tggaaagtac caccaccagg gcgatagtga ctgaagctga cttgtaattc
    11521 tttagctcga atatctgcag cctgttgaac aatatcattg aattgcattg gggcaacacc
    11581 attttgttgg cgataatcat ttaaccaagc aagttctttt tgacgcataa agttacgtaa
    11641 ttcgtctaat gaaatctttt tatatttgaa tgtgattgtt ttattttgat cgacgttatt
    11701 aatggatcgt tggccgtttt tatccacggt aacggtagga tcgtcatttc tgcacaagaa
    11761 ataattttga tcagtcatgt ttttccaagg agtagcaaag ctatcccctt cgtatactgt
    11821 ttttgattca ctagagagaa cagcgttggt atcagcattt acatgattaa cagtcacatt
    11881 aaatttacga gtgtagttga aaatataatt ttgactttca gtcacattgt ttaaggttac
    11941 tgaatttgtt ggttctaccc gatcgtctaa aacatatcct tgaatagaag gagcagttaa
    12001 agtgacactg tcaccgtgag tgacattgac tatctgttct ttcaaaatat taactcgtcc
    12061 ttcgtttttg tagttgttat tgcacaacaa attgatttga tgcacttgat tataattgaa
    12121 tgtgattgtt gtattacctg tgattgtttt gctttgagta tcattaccaa tcactttgta
    12181 gctaaaatta tctttagcgc cttcgctata tttaaatgct tttgccttag cagtgtaagt
    12241 agaaccagaa gttacttttg cttgatctgt agcaatcact tgacctgttt gattgtcaat
    12301 atggttaata gtaaccgtat attcttttgg tgtttcaggt ttgtgttgca accctttaaa
    12361 tgcagcatca agactatttt tagcattatt tacttgggct tgagaagcct tagaatcgtc
    12421 tagtactttt ttagcattgg ataaagcagt ctgaaaatta ttccaagatt cttctgtaaa
    12481 atctccttta gcagttgatt ttactcggtt gtataaagct tgtaattcag ctttattcac
    12541 agtaggaact ggttttttct ttaatccttt gtaagcagaa tctaatttat ttttggcact
    12601 atccacttgt ttttgagtcg cttttgagtc atttaacact ttcttagcat tgtttaaagc
    12661 ggtttggaat gcgttccagg tattgtcggt atagtcacct tttttagttc ccttaacgct
    12721 cgtataaaga gcttctaaag ctgatttatt cactttgggt ttagaggtgt cagcgccata
    12781 ccaaccgata ccttcgtatt tccagccata gtctttggtt aaccattgta cttctttcca
    12841 acttgcagtg tagtgatgtg agccagattt tacaccagga aggaaaaggc ggtagatcgg
    12901 tttattattt ttttcggcag aacgccaggc aggcccttca taagtccagc cgtaattttt
    12961 agtcaaccat ttcacttcat cccaactagc tgtgtaatgg tgatcacgaa gacctttatt
    13021 gtacaaacga tagacaagat ggccaccgct agtcggtgct tcccaggcgg ggccttcata
    13081 atttccccat tttatcttta ctagatggtc acgttctttt ttgctagagg tgtaaaaatg
    13141 ttcatgatta tttggattgt aaaggcgata catgacgtta gttgaagcag cgtcaacaga
    13201 tccagcaaga ctaagtgagg cagcaaatgt tccaatagct aaaacagttt ttggattaaa
    13261 taatttcatt ttttcaactc ctaaatttaa ttaaaaatgt ttacatgatt attatatatc
    13321 ggctaaccga tatagtcaat aaaaaataag aaaagcctga tataattttg gcttttctta
    13381 ttttatttgt tagttgaagt attaatatca acgatttctt cagccaattt atttaatttc
    13441 atagcattta tgaatctcat gttgtctata ctagagcgtt tgttggcaag gtcactgact
    13501 gtagttaagg gtacgccaga ttctatggat atagaacgaa ctgatttacc actatcaaac
    13561 agcaaccatt cgattttctt tttatcagcg tacatttcaa tgctccttct tgttttttct
    13621 ttattatagc tcttttttgt aataaatcat aatatcaaga gaaaaatttt ctatagaaag
    13681 gtggattttt atgagtattc gttctccgtg tatccaaatg aggtttgttg ctactttcgt
    13741 tatttgatat actaaaaata aacaacgaaa gaaggaataa aatgaagaaa aagcaactat
    13801 ggtttgtctc tatgtttgca atatttttta ttattttaag tggatgttca agtaataaga
    13861 aatcggaagc taaagaagat aatagtttat tgcaaccagg aactgtttgg aaagaagaag
    13921 taggtggtct tattcataat ttaaaaatta tagatgatac tacctgggaa tattctgaaa
    13981 gtgtatggca tccagatcct gtacaaataa cagttaagag acaaaaagac tatgaagggt
    14041 tagaaagata caaaatagta gattctgcag gtatcagaaa atttataaac aaacccgatt
    14101 ctttatttat cgttgttcct tacgagaaag atggcgtaaa aaaaattgtt tttcttggat
    14161 ctagtaagga tgaaaagcga acgaaagaaa aattgattca tgatggatca caatcaaata
    14221 aatacaaatt acaaaagact agcgagtgat tgctagtctt tttatttacc ctaaaggagg
    14281 aatcatagtg gcaaaagcta aaacgccatt tgaaaaagcc gcgtctaaaa tcttagaacg
    14341 tcgcggtgta tctgaaacac agtggaaaca agaagtcctt gaaaaagcac aatttgacct
    14401 attacgggga aaagacaagg acttagaagg atatatctat caacgtgaac gtgaaaagct
    14461 tgttttcgat gaaattatta aacactaaaa ggagcgaaca attttatgtt tgaatcaatt
    14521 acaatgaatt ttttaacaca accgaaagta gtagctgcgg ttgatatttt tagagcatta
    14581 caaactgccg caactagttt aaaaaacaat ctgatgattt ttgctgctgt tgcggcagtg
    14641 gcttccattg ctcttggagg tattttcttc ttctttgggc gaagaggagc tgaaaacggg
    14701 aagacacacg taggttatgt attgttgggg gtaggagtta ttttagctgc cgcttctatt
    14761 gtagtttcaa tgttctcaat gtttggacaa accccacaag gactataaaa aaaacaagta
    14821 gaaggagtaa aaagtacgtg gctacttttt actccttttt ttatgaaagg agaatattca
    14881 tgttttttac gatttcagga aatgcaaaat tggataaaga gttaataaga aaagcaacga
    14941 agaaagtggg aaagcgtact tcctttttac cgaaaacaat taaatattct gtcattgaaa
    15001 cggaagaaca gctacatgtg ttaggacaag tgttagaaca agagaatcaa tcttctgtta
    15061 aattgactct cacgttacat gataaagaag gaaaaataga aaaggctggc acagaatacg
    15121 tttttggccc actggattat caaatttcag aaggttttta tcctacattg atggatttaa
    15181 tccgccttga tgtgtttaaa gatacggatg aaactatgag tgtggaagag tcttatcgaa
    15241 acgaaaaact tttacaagtt ttagaagaag agttagagag cgaccaaaga aaagtgaatc
    15301 aagtgagtga agtcgaagta aatgaagaag taccatggga acgaccaata tttccaaaga
    15361 aggagtcaaa agaaaaagag acagtctatg ttgacgaaca agctgatatt ccgactcaga
    15421 aatcgatatt tgaggaagag aacgatgtga tgggtcaatt ggaggtagat ccctttgaag
    15481 aaacaatgga ggatgaagag acggacgaag tagaagttga tccagttgaa gctaatgaag
    15541 agtcaacatg gactgaaggg gattctcagg aatatattga accacttatc agtgaagaag
    15601 tagaaaaaaa cgaaacagag atacaaaagg caactgtatt tgattatatt ttggatcaat
    15661 atccgccaga gcatgaatgg atgaaagaaa aaaacaaatt atttttaaat gagctttatt
    15721 cgaaatatca attaccccaa acctatcaag catttttaga gagtaaagaa gaattaatct
    15781 cacagggtaa aggcacactt ttagaacagt tagagtctgt ccaatcaaca aattggcatg
    15841 agcttgcaca agaagattta ttagccacct ttgaagaacg tcaacaagaa acagatcaag
    15901 aaattaatga gtatgcgacg gaacagtcgt ctaattggga gaaagtaaaa gaggaactaa
    15961 ataaagaaga acagcgagtg attgaagaag aagtacaaaa aatcaaagag cggtacgaaa
    16021 agaaacgcga caatgcttat tccttgtgtg tagaaaagat caacaatttt actaagacga
    16081 atcaagaagc gttatcgaaa gaaaaagaaa acttgttaaa agaacgtatt aaagaacgaa
    16141 aagaagagtt ctaccgcggt ttacgtacag ataagctaaa gattagcaaa gaattaaatg
    16201 aacaactgaa taagctatat caaacaacga ctgaagttct tatggaaaaa cacgaacaga
    16261 tccagcaaga attacaagta caaattccaa aatggaaaaa agagtatgaa attcaacaac
    16321 aagaattgaa agaacaagaa gaaaaacaac ggcaaaaaga aaaagagcgt gaacaattag
    16381 aaataagaaa acgggaacag gatcttgcgg aacaaaaaat ggcgatagaa aaacaacggt
    16441 tagagcaaga aaaggaaaaa gaagcagctg ctcgtaagga acgtcaagaa caaatggata
    16501 cattgcgtca agcacagttg aattatctag cacaacctca accagtagct gctgttcaaa
    16561 cgccagtgaa caaccaagca aatttaactc cttcacccca gcgttccttt aacggatgga
    16621 tggtgggatg tattgcaagt ttaagcttgc ttctaggtgg aggaacagta tttgctttta
    16681 atcatatcaa tcaatcttca caggctgatt ctgtagccac aacggcgtca atcaaggaag
    16741 aagcccagga acaagcaaga aaagagtatg aacaacaatt agaagaatta ttgaatcgta
    16801 ccaatcagtc aacaacagag tcaagcatgg aaaatagcaa agagagtgat tctagtaagg
    16861 ctaaagaagg atcaagcgat tcaactgtac aatcaaacac ttccgaaagt tcagaatcaa
    16921 atgaggtgaa ataatgcgtg ttagagaaac aacgaagcga aaagacaaac gattcaattt
    16981 agaaaagaat aggtatcttt ctcatttcta tcgctatact ccctcaggag aagagctacc
    17041 agaaaaacat gacaaacgca tttcgaaaaa gattttttat agtggacttg tatacctccc
    17101 tttagagtat ttagtaacaa gagtaccaga agttttcaac cgattggaaa ttgttaaggg
    17161 aagcttacaa ggaataaatc tgtttcaatt acaggaaatg ccacaggtct ttcattttga
    17221 ttcagctggt ttttggaatc catttactcc tacaactagt ttgggtacac gtttagcaat
    17281 ttcgacagtt tgtgtggttc cagctgtact tatgggattg aaaatggaca tgtggtttcg
    17341 tcctctagca gatggacaaa aaggggataa tcgattaatg accttaaaag aaatccaaca
    17401 gatttatccg caaatacctg aaacaaaaac tacttttcca ggttatggag gtattccaat
    17461 cacacattac aaaaaatatt ggtacattca aacagatact gttaatacgt gtattgttgg
    17521 tacctctcga tcagggaaag gtcagattga agtgttagcc accattgaca atttatctcg
    17581 tgcagaaaaa caaagttcaa tggtcgttaa tgatccaaaa acagaattat atgttggtag
    17641 taaagatgaa ctagaagcta gagggtatga tgtatatgcc tttaatatca ccgatccact
    17701 gcaatcaatg agtgataatc cattggcact catcgtgaaa tactggaaac gaggagacat
    17761 agatactgca acacagttaa ccaatacttt cacaaatacg atttattatg atgccaacgc
    17821 ttcagataac aaatacttta acgaaaacgc gcagaaagct gtcaatgcgc ttatttttgc
    17881 gttgttagaa caagctgatc aaacaggaga ttactccaaa ttgacaccga ataatctcgt
    17941 tgagcttctt tctgagattg gcggttttaa ttattatcca gatccagaca atgaattcaa
    18001 acagttgaat gccttagatg aatttatgaa tcagttgcca cctggaaaca ttgcaaaaaa
    18061 acaatatggg gcaacgaaaa ttggatctga taaagcgaag ggaaatattt tatcaacggc
    18121 aatcacggga ttgaatccat ttactctaac gaaaattgca aagatgactt ctcaatcaac
    18181 gattgattat aaatcggttg ggtttccaaa atacttagat ttgaaattac atgaaagtct
    18241 tttaaatcaa cgagtcgtag tagaatttta tcataaggga aagaaatttc atgaagaacg
    18301 tgtcaaagta ggctataaag gattttgtga aatcaatttt gattgtcatt tagttgcagg
    18361 tgatcaagtt tgtttaaaat acgatcatca aggaaaagaa tatgtggctt ggtatgaatt
    18421 ttctcaaaga gtattgaaag acgaagaggg taatatcgtt tatcggaaaa aacgaggaga
    18481 aacccatttg ccagaattag aaaaagaagc cgttctaaca ttagatgagg agaaaagtaa
    18541 cttattagta gagtctattc ggatgcacta taccgataaa ccaatggcta tttttatgct
    18601 tacacctgat tatgatccat ctaaccatat cattgttagt atttttctta gtcagttata
    18661 taaagaatta tctacgcaat gtgttaagac taaaggagat aaatgtcacc gcaggattca
    18721 ttttatttta gatgaatttg ggaatatgcc accaatggat aatatggaag gaatcatgac
    18781 ggtcacagct ggccgaaaca tgttgtggga tctgtttatt caatcttatc agcaacttta
    18841 tgcgaagtat ggagaacaaa acgcagcgat tatcaaagaa aattgtcaga cacatattta
    18901 tattatgtcg acagatgata ataccatcga agaattttct aaaaaagtgg ggaataagac
    18961 ggttgaacaa gagaacagta ccatcaattc attaggtatg aatactcata tcaatcgcaa
    19021 tgttgattca gatcgtatct taactcctga aagaatttct acttttttag aaggtgaaac
    19081 gattgtactc gctcctttaa aacgacaaga tttgaaaggt cataaaatac ggccgtatcc
    19141 tatttttaat catggggaaa cgaatcttcc ttatgcttat caatatattg aagatttcaa
    19201 tccatcgtgg gatatcaatg atatagacat acattgtaca catgcggact tggatcttaa
    19261 ggctttacaa attgattatg attatttcct accagatgaa tatgctagaa atctctatca
    19321 tggactgcat gatcaattag aagaaaaaaa cgaagaaaat atgttttata aaatcgttca
    19381 atctcttgag aatgagacgg ctaaaaaatt gttaattcaa ttttacgaag aacaagcagc
    19441 agatcgactt caagaatatg ttgaaaagtt gatcaaaaat aaagaaattt ctgaaatgat
    19501 tgggaaacgg ttgttattgg aatctgaaaa ggtggtgaaa gaatgatcat tggaggaatt
    19561 ttttctggca tagccgactg gtggtcaggc ttattcggag aaggggatgc aaaccccaaa
    19621 accgtaacga aagtcttac
//
LOCUS       DACKAC010000050        19494 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.050, whole genome shotgun sequence.
ACCESSION   DACKAC010000050 DACKAC010000000
VERSION     DACKAC010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.050"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            127..3654
                     /locus_tag="I0B46_001909"
     CDS             127..3654
                     /locus_tag="I0B46_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAR2084191.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3651..7373
                     /gene="addA"
                     /locus_tag="I0B46_001910"
     CDS             3651..7373
                     /gene="addA"
                     /locus_tag="I0B46_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAR2084192.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7442..7783
                     /locus_tag="I0B46_001911"
     CDS             7442..7783
                     /locus_tag="I0B46_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAR2084193.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8092..9135
                     /gene="pheS"
                     /locus_tag="I0B46_001912"
     CDS             8092..9135
                     /gene="pheS"
                     /locus_tag="I0B46_001912"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAR2084194.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9140..11560
                     /locus_tag="I0B46_001913"
     CDS             9140..11560
                     /locus_tag="I0B46_001913"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAR2084195.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKEINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="I0B46_001914"
     CDS             complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="I0B46_001914"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAR2084196.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12994..13731
                     /locus_tag="I0B46_001915"
     CDS             12994..13731
                     /locus_tag="I0B46_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAR2084197.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13745..14560
                     /locus_tag="I0B46_001916"
     CDS             13745..14560
                     /locus_tag="I0B46_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAR2084198.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14573..15220
                     /locus_tag="I0B46_001917"
     CDS             14573..15220
                     /locus_tag="I0B46_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAR2084199.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15236..15892
                     /locus_tag="I0B46_001918"
     CDS             15236..15892
                     /locus_tag="I0B46_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAR2084200.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16054..16875
                     /gene="racE"
                     /locus_tag="I0B46_001919"
     CDS             16054..16875
                     /gene="racE"
                     /locus_tag="I0B46_001919"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAR2084201.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16878..18233
                     /gene="rph"
                     /locus_tag="I0B46_001920"
     CDS             16878..18233
                     /gene="rph"
                     /locus_tag="I0B46_001920"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAR2084202.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18234..18752
                     /locus_tag="I0B46_001921"
     CDS             18234..18752
                     /locus_tag="I0B46_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAR2084203.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18859..19359
                     /locus_tag="I0B46_001922"
     CDS             18859..19359
                     /locus_tag="I0B46_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAR2084204.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6560 a         3347 c         4183 g         5404 t
ORIGIN      
        1 cgtatactta aaaaagtaga aatatcagaa ccttaaaagg atataatttg aagaatatag
       61 aacaaatgtt cagtattttt tgatataata aaggagcacc gtatcatgtg ttgaaggagg
      121 aaaagcatgt ctttacaatt tattcttgga aacgggaaaa aagatcaccg tcaagtcttg
      181 atcgacgaag catccgcttg gctcgaccag gatgcaagta accaagtatt tttccttgtt
      241 cccaactaca gcaaattcga acaggaacaa gaaattttag cggagatgcg aagaagaagc
      301 ggaaaaaaaa ctttcagcac aacaaacgtt caagtattta gtttttatcg tctggcttgg
      361 tatttgctgc aacagacaac tcttcttaca ggaaatgaat tatcagaatc aggatctgca
      421 atgatcttgc gacagatttt agaaaaaaat gaggaagaat taacaatctt tcgcggagaa
      481 atcaataaaa gagggtttat tcgtcaatta caagaactct acagtgaatt gcgtacagga
      541 aatattcaac cagaagacct gatgctttta tttggtgatt cacctaatgc agaagaccag
      601 caacttaaaa tgaaagactt gaagctgatt ttttcagctt atgacagcga attgactcaa
      661 cgtgcactaa caaatgaaga tgcactatct atactagcaa attatatgca gacacaagat
      721 ttcaacaacg tcaaatttat tgtcagtggt ttttctcgaa tcaatggtca agagtatcaa
      781 ttactgcagg caatgatgga aaaggggcaa ttggttgtcg atttattgct ggatcgtcct
      841 tatacagcgg atcttccaga agtacttacg ctctttcatg agactggaaa gctttatttc
      901 cgtctttttc aaagtgcaag agaacggaaa gtccctatat tagtggaccg atttgcacca
      961 gagcagactc gtccgacaga gcttcaactg ctgcaaacaa tgtgggaaga gacatctagc
     1021 caaaagaaag cggcagtaca caaaataggg ggacaagagc atctccattt gtgggaagct
     1081 gaaaatccaa ctgaagaagt ccgacaatta gctgttgaaa tcagacgact agtcagtgaa
     1141 gaaaactacc gttatcgtga tatccaagtg ctgacaaaaa atatgtcttt atatggaaat
     1201 atccttcggc cgatctttca agaaatggca attccttttt atatcgatga agaacaaatg
     1261 atggaaaatc atccgctgat cgaatggatc aacagtctgt tcgctcttga ccgctattct
     1321 tatcggctaa gtgatattat gcgttttttc aaaactgaat tatttctgtc gacggaagca
     1381 gaaaccgaac ttgatgaatg gaaacaagag cgagatgaat tcaggaaaaa actggacatc
     1441 actgaaaatg tagcacttgc ttacaattat caaggaactt attggactcg tgaaaaagac
     1501 tggcagtttg tggcgtatga ttttgaagca gaacagattg aagacgtaca gtatttggaa
     1561 gaacagtcaa atacgattag acgattattt cagaaagctg taccagtatt tttgaaaaaa
     1621 ataaagaaaa gcaaaacagg acaagaggcc gcaaatgttt tttatcaatt tttgatccaa
     1681 agtggcgtac agcaacaatt attgttttgg cgtaatcagg aagtagacag aggaaactta
     1741 gaagcggcaa gaaaccatga gcagacatgg ggagcgctga ttgatctgtt ggatgaatat
     1801 gtagtaattt atggtgaatc ggcttttgac tggtcactgt tccaagaaat cattacaagt
     1861 ggtctagaaa atctaagtta tggtaaaatc cctaccgcaa tcgaccaagt gcgaatcaat
     1921 cgtttggaat tagttcgacc aaatcaagct gccgtcactt ttgcattagg gttgaatgat
     1981 caagtgtttc cagaccgaaa agagacaaaa ggattgttgt ccagtgaaga acgagaagta
     2041 ttaaatcagg ccttgccaga tggacagttt ttattcgacc catcaaagga aagtatctct
     2101 tttgagcctt tccaagccta tcttgttttt ctttccggaa cagatcgatt atatcttagt
     2161 tatgcacaaa gctatgacac agatggttcg ctgaagatgt ctccttattt gcgtagaatc
     2221 acagaatatt tatctgttcc cgtcgagaga aaagagcatt tgacgatcga aagcgaccct
     2281 aattgttatg tagggactta cagaagcgga atcaatacag tcaataggat ttatcgatta
     2341 gctttagatg aaaaaaaacc tgtaccaaag cactggcaaa agctcaaaga attagtactg
     2401 aattcgtcat ggcgaaattt tgctttacag atttttgaaa gtcaggaaca tttaaatgtt
     2461 cctgtttcga tgccagaaaa aatggcggaa gagctttatg gaaaagagat ttatacttca
     2521 atttcacgta tggagaattt ttataattgt gaatacaaat acttcatgca atttggtttg
     2581 aaattgaaag aacgaactgt ttatggtcta actccagcag caactggtga tttttatcat
     2641 gaatcattgg atcgtttctt caagctactg ttttcaaacc aattatcctt agtgaatatg
     2701 tccgatgcgc agcgtagaga ctttactgaa aaagtgctgc aggaagtatt cggagaactt
     2761 cggtttgaga tattggatag ttctgcaaga atgaactata tccgctacca gttaggacag
     2821 acgattcaaa aagtgacatg ggctttgcaa aaacagagca aaaatacagg catgcatcct
     2881 cttcagacag agattctatt tggtcagatt gctggagcaa aaggtatcac aggtctggaa
     2941 ttaccgttga gtaatggtgg aaaactccat gttcgaggga aaatcgatcg aatcgatgta
     3001 gcttctgaac aagaagatac ttggctaagc gtcgttgatt acaaatctag cggacgttca
     3061 ttcgatgtca cagaagctta ttatggcatg gctatgcaat tattgacgta tctggacgta
     3121 gcattgatgg atgcggtacg tttgactgga aaggcggctg ttaaaccagc aggatcatat
     3181 tatttacatg ttcataatcc tgtattgacg gagacagatg atgtagagaa gaatacattg
     3241 aaaaaattta gttatgatgg cgtttttgtc gatgatcccg ctcttttaga agtactagat
     3301 cattcactgg aagcaaaaga acaatcactg atctatccag tgaaaaaaaa tgcgaaagat
     3361 gaataccaaa aaggtagttt cagtaaagac aaatttttca cggaagaaga actgcaacta
     3421 ttcatggcgc ataatcggga aaatatgcgg ttagctggag atgcgattac ttccggagaa
     3481 atcaaactaa atccagctta taaagataaa cagcggattg cctgcgaatt ttgtccattc
     3541 agaagcatct gtacgtttga tgtgatgctg aaagaaaata attaccaccg tctggagaaa
     3601 atagataagg aagaagcact gcgtagaata ttgaaaagga gtgaagaaga gtgaatcaaa
     3661 ttcctttaaa accgcaaaat gaacgattca cagatgatca gtggcaagcg atttttgatc
     3721 aaggagataa tctattagtc tctgcgtctg ccggatcagg taaaactaca gtgcttgttc
     3781 gaagagtgat tgaaaaacta aaaatgggtt ttgatatcga tgaattattg attgtgacat
     3841 ttactgaagc tgccgcaaga gaaatgaaag aaagaattca agaggcatta caagaatccg
     3901 tcaatagcga aagtgaccct gtacgtagac agcattttac taagcagtta gttttattgc
     3961 ccacagcgaa tatctcaacc ttacatgctt tttgcctgac agttattcga cgttattatt
     4021 acttaattga tattgatcca gtctttcgca tgttaacaga tgaaacagaa acgatcttga
     4081 tgaaggagga tgtgtgggac gagctgagag aagcacttta tgcagaaaat gatgaacgat
     4141 ttttccaatt aacaatgaat ttttctaatg atcgttcaga tgacggcttg acgaatctgg
     4201 tgttttctct ttatgagttt gcccgtgcca accctgaccc ccaaaaatgg ctagaacagt
     4261 taagcgataa ttatcgtttg cctgaggggc tagcgaaatc tcgattatat caaaaacaaa
     4321 tccgtccttt agttttggca gatatatacc aatgtgtaca gttatatgaa cagatgactc
     4381 agttagcaca aggtgaagga ttagaaaaaa tgcatgagca agtagctgga gaacaacagc
     4441 agatcaagaa tatctatgaa gcgttctcgc aagatcggtt agaagaagct tatgctggtt
     4501 tggaacaact aactttttca acttttaaga gcagtcggaa agcagaattg aaagaaattt
     4561 ccaatgaagt caagggcatg agggataaag caaaaaaact gattcagcag ataagtaaaa
     4621 gttattttcc tgtttcccca agccaaatgg aagaattaac ggacaaagca ctaccgttag
     4681 tcgaagaaat gacaaaagtc acgcaaagct tcatggacgg tttcagtatg cgtaaacggg
     4741 aaaaaggtgt gctggatttc aatgatctgg aacatttagc gttgcagatt ctcacagaaa
     4801 aaacgaaaga tgtatggctg ccaagtgaag cttcaaaaca ttatcgaaaa aaattcaaag
     4861 aagtaatggt agatgaatat caagacgtga accaattaca agaagccatt ttatactggc
     4921 taagagaacc cgatgataca aaaggaaata tgttcatggt tggggatgtc aaacaatcca
     4981 tctattcttt ccgtttagca gatcctagtt tatttatcgg gaaatacgag aatttctcaa
     5041 agaaagaagg cggacgtcga atcgttttgg ctgagaactt ccgctctaga aaagaagtcc
     5101 tttcttttac gaatcttatt tttgaacaac taatggatcc agcagtgggt caaatcaatt
     5161 atgacgaagc agccaaactg atccaaggat tttcagactt tccagagaat gaacagtttg
     5221 agccggaaat catgatctat gaaaaagaac aggaagaatc agagattgag attccgacgg
     5281 atgatatact ggaagacaaa acagaaggcg aactttttat gacaggacta aaaattcgtc
     5341 aattaattga ttcctccttt atgatttatg ataaaaaatc aaaaaaaagt cgtccgatag
     5401 agtacaaaga cattgttctc ttgacaccta caaagaagaa caacttaacg attttagaga
     5461 ttttcaaaac attggatatt ccattagaga tgaatgatgc tcaaaactat tttcaagcga
     5521 cagaaattcg cacgatgatc tcgctgctac aattaatcga taatccctat caagatattc
     5581 cgcttgcggc tgtattgcgc tcaccgatcg tgggtctgat tgaaccagaa ttagccagca
     5641 ttcgattagc taaccgtgca catacttact atgatgctgt gcttgcttat caagcatcaa
     5701 acgaagatga attagcagct aagctggaac attttggcaa gcaactagag cattggcgag
     5761 aacttgctcg ccgttcttcg atcactgact tgttatggga tatctattat gagacgggat
     5821 atctagagta tgtggtcggt ctgccagcag gggcacagag acaagccaat ctttatgcat
     5881 tggttgatcg tgccaaagct tacgagcaaa gttctttcag aggattatac cagttcgtgc
     5941 gtttcattga aaaaatgcaa gaaaaagata aagacttagc agaaccagtc atttcaatag
     6001 aagataatgc tgttcgcgtt atgacgatcc atgcaagtaa aggcttggaa ttcccagttg
     6061 tatttttgtt agatatgaca aaggagttca acttacaaga cttgcgcaat cggtatgctt
     6121 ttgaagaaaa acttggagca ggaattcgct atatggatcc tgaaacacga gtgttatatg
     6181 atacgttacc gtttcaagcc atcaaattgg caaagcaaaa caaattgttg tcggaagaaa
     6241 tgcgtaaatt gtatgtagga ttgactcggg cagaacaaaa gcttttcatt gtcggttcct
     6301 ataaaaataa agagcaaatg atccaaacgt ggtctgaagc agctgatcat gaggagttag
     6361 tttttgatcc tgcgctacgt ttgaagggca gaagcagctt gatgaattgg attggttacg
     6421 gattgatccg tcatcctgaa atgcagaaat acttagagga agaaatttcg acttctcttc
     6481 ttcaacattc aaatgcacag ttttctattt catggatgaa tcagcaatcg atcattgaac
     6541 aaagacagtt gcttgctgaa aaagaactgg ttaacttaga tcagcagatg aaagaagatg
     6601 aaactttatt agcagattcg ttacaaaaac gtctggcata tgaatatcct tatcaagctt
     6661 cttcacaaac cacaagttat caatctgtat ctgaaatcaa acgattattc gaagatccag
     6721 atgatacaca agaatcacgt ttgactctgg aaagcagtca gaacaaagca gcatcccgtc
     6781 aatttcgtta tacgcaagaa caactagcag aaccaaaatt tttgcaaaaa gatcggcaag
     6841 tgtcagcagc tgctgttgga actgctacgc atgctttgtt acaattactg ccattagaga
     6901 tgccaactac agagagtatc catcaaaaat tacaggaatt ggtaaggaaa cggttagtag
     6961 acgaaaaagt cgcgaaaaaa gtagatgttt cttctattat atggttcttt caaacagaac
     7021 ttggtcaaca gctgattgcc aacaaagaaa acgtaaaacg agaacaacct ttttccatgc
     7081 tgcttcctgc tgacgaagta tttcaagact atccaaatca agaagatgaa ctattaatcc
     7141 acggaatcgt cgatggatac ctagaagaaa aagatcatct caatatctat gattttaaaa
     7201 cagattttat tttacatccg gatgatccag cagaaataga tgcaatcgtc caaaaatatc
     7261 aaggacagct aagactttac caacaagcaa tgtcagaagc tttgaataag ccagtagaaa
     7321 atgttttcct aattttgctt cgagtgaaac aaattataaa cattaacaaa taattataat
     7381 tgagcaaact tgacttatgt tttgtaagga ttatgctata ctctgggaag aggtgagtat
     7441 gatggagcta acacagacag aattcgtttt atgccctaaa tttgaaaaaa cattctctat
     7501 tttaggaaaa aaatggaacg gattgattat cgatgtacta ctcgaaaacg gtccgcaacg
     7561 ctttgttgag cttgcggcaa aaatcccgat ggtcagtgat cgagtattag tcgaacgctt
     7621 gaaggaatta gaaaaagaag gaatagtcac tcgaaacttt gcctgtagcg aaaagagccg
     7681 ttcagaatat ctcttgactg aaaaaggagc agacttgcaa aaagcgatgc gtcaaataca
     7741 agtttggagc gaaaaatgga tcacagagga agaatgcggt tgattttgta aaaaaaatct
     7801 tgtaaactat agtccatacg aataagtaaa gaacgatgac gaaaaagagt agtttggcta
     7861 aaccagttca gggagatctg tcgcagactg aaagcagatc ctgtgtttga aaaatgaagt
     7921 tcactttccg agttcgtctg ataaaatcag accggcaatt gtcgttaacg aagtaagagt
     7981 gcggtgtaag tcatttatcc gcaaattagg atggtaacgc gaagacctcg ttccttaaca
     8041 ggaatgaggt ctttttgttt tgtatttgga atttaaagga ggaacaagca catgtctcta
     8101 caagcacaat tagagagctt gaaagaagca acgctcgtca aaatcacagc ggcacaagat
     8161 ttaaatgaat taaatcaaat acgtgttgaa acgttaggaa aaaaaggacc gatcacagaa
     8221 gtattacgtg ggatgaaaga tctaagtcca gaagagcgac caaaagtcgg aagtttcgca
     8281 aatgagatcc gagatctttt gacagaagag atcgagcgaa aaaaacaagt attagaagaa
     8341 gaagcgttaa atgcagcttt ggcaaaagaa acaatagacg tgacactacc tggtagaaaa
     8401 gtaaaacatg gtacacgtca tatcgtgaca caaatcatgg aagaaattga agatattttt
     8461 gtgggtatgg gctatcagat cgtagaagga tatgaagtag aatctgatca ttacaatttc
     8521 gaacggatga atctgccaaa agatcatcca gcaagggaca tgcaagatac tttctatatt
     8581 tcagacgaga tattgattcg gacacatact tcaccagtcc aagcacggac aatggaaaaa
     8641 catgatttct ctaagggtgc tctacgaatg atctcgccag ggaaagtctt ccgcagagat
     8701 acagatgatg cgacccacag tcatcagttc catcaaattg aagggctagt tgttgataaa
     8761 aacatcacaa tgggcgacct taaaggaaca ttagaagtcg taatgaaaaa aatgtttggg
     8821 gaagatcgtg aaattcgttt gcgtccaagt tatttcccat ttacggaacc atcagtagaa
     8881 gtagatgtca gctgtttcaa atgtggtggt gccggttgta acgtatgtaa atacactgga
     8941 tggatcgaaa ttttaggagc tggcatggta cacccgaatg tgttgaagat gtcaggaatc
     9001 gatccagaag aatattcagg ctttgctttc ggccttggac cagaccgcgt agctatgttg
     9061 cggtatggag tgaatgatat ccgtaatttc tatcaaaatg acttacgttt cttaagtcag
     9121 ttcaaggggg agtaagaaaa tgttagtttc atataaatgg ctcaatcgtt atgtagattt
     9181 atcgaatgta acaccaaaag aattagcgga taagatgtct gtgacaggaa tcgaagtaga
     9241 gggagtaacg gttccagaag aaggcttgaa aaaaatcgtt gtcggtgaag tcaaggaatg
     9301 cgtaccacat ccagattctg atcacctttc tatttgtcaa gtcgatattg gcgaagacga
     9361 actgtctcaa atcgtttgtg gagcgcctaa tgtgaaggtt ggaatcaaag tgattgttgc
     9421 acttcctaac tcacgtatcg ctggtaatgt aaaaatcaaa aaaggaaaaa tgcgtggcca
     9481 agtatcaaat ggcatgattt gctcattaca agaaattggc tattcagata gtgttatacc
     9541 gaaagaatac gctgaaggta tctattatat gccacaagaa gctgtaaatg gcgatccagt
     9601 ttttccttat ttggatatgg atgattcgat tattgaatta tctatcacac cgaatcgtgc
     9661 ggatgcattg tcaatgcgtg gcgtagctta tgaagttggg gcaatttatc gtcagactcc
     9721 tacattcgat gacgaaaagc tggtagaatc ctcacgtcct gctagcgata aaattcaagt
     9781 ggaagttgaa gataaagaag cagttcctgc ttatcaaatc agaatcattg aaggagtaag
     9841 aatccagcca agtcctcaat ggctgcaaaa tcttttgatg aacgaaggca ttcgtccaat
     9901 caacaatgta gtagatgtga cgaactatat tttgctttta ttcggacaac cactacatgc
     9961 atttgactac gacaaattga aatcaaaaga gatctttgtc cgccgtgcaa aagaaaatga
    10021 aacattagta acactagatg gagaatctcg ggaattgaca actgacaatc tcgtcattac
    10081 taacggcaaa gaaccagttg ctttagctgg tgtcatggga gggttagact cagagatcac
    10141 gaatgaaaca acaacagttg cactagaatc tgctttattt gatccaattt cgattcgtcg
    10201 tacagcaaaa caatttaatt tgcgtagtga atcctctgca cgttttgaaa aagaaatcaa
    10261 ccatgcgact gttggagaag ccagtgaggt tgctgcagca atgatcgttt ctcttgcggg
    10321 tggggaagtt ctacaaggcg cggtcaaagg ctcagagatc aaagcagaag acgtatcagt
    10381 gaagatttca cttgaacgca tcaatcaata tttaggaaca tctcttgtcg ttaaagaagt
    10441 aaatgagatt tttgaagcac ttggatttag ctatgaagaa aaggagtatg tttattccgt
    10501 agtagtacca ccaagacgct gggatatcca aatcgaagca gatattatcg aagaagttgc
    10561 tcgtatttat ggatacgacc agttgccatc tacacttcca agtggagaaa cggttgctgg
    10621 aagcttgtca aaagaacaag aaactactcg taaaattcgt acaatctttg aaggaagcgg
    10681 attgagcgaa gcaatcagtt atgcattgac aacagaagaa aaatcaagac agtttgttat
    10741 aaatgaaagt aatattacac gtttggactg gccaatgagc gaagaacgtt ctgtattacg
    10801 aatgaatttg atcagcggac tattagatga tatcagttat aacactgccc gaaaaaatac
    10861 agagattgct ttatatgaaa ttggtcgggt gttcttccaa gaaaatgatc cacgtacaca
    10921 tctgccgcaa gaagtaaaac atgcagcaat cgctatttct ggtatttggg aagaaaaaga
    10981 ttggcagacg aaagctcaaa atgtagactt tttcaccatc aagggtcttt tagaaaactt
    11041 atttgaacaa ctaggcatca ctgaagatgt ccattatcaa acagtgacag aaatgaagga
    11101 aatgcatcct ggaagaactg ctgcgattta ccttggcgaa aaatttattg gttttgtcgg
    11161 tcaagtgcat ccaacgatta caaaaagtta tgatattcca gaaacatatg ttgctgagtt
    11221 tgatttatct gctatagtgg aagctgctca aaaagggatc gttttcgaag cagtgacaaa
    11281 attcccagca gtcagccggg acatcgcttt gcttgtaaaa gaaactgtga caaatcagga
    11341 attaacagaa gttatccgtt ctgctgccgg aagattttta acagatatcc aattatttga
    11401 tgtctatcaa ggagaaaaca ttgaaaaagg acataaatcc atggcgtata gcctgacatt
    11461 cgtcaaccca gaagcaacat tgacagatga agaaatcaat aaagcaatgg aaaaagtcga
    11521 aaaagcgttg gtagaaaacc tagaggcaag tattcgctaa gaaggaaaat atagtaagta
    11581 acgatcaagt agcttttgaa gaccatacat ttgaaaaaaa gatgtgacac aacttcgtca
    11641 catctctttt tttgatttta aaaaaggaat agcacagaga cgagataaaa aaacaggcct
    11701 ttacagcctg cttttttaga gtaaatgaat taatggaagt aattgattcc cattgctgat
    11761 tttacttcag aaagtgtttg tgcagctact ttagcagctt cttcactacc tttacgtaag
    11821 atttccataa cagctgaagg atctttttcc aattcttctc ggcgcgcacg gatcggtgca
    11881 aattcagctt ccaatacatc aatgaggtat cgtttgatct ttacatcacc aagtccgccg
    11941 cggcggtaat gagctttcat ttcttcaatt gcttctttat ctgttccgaa tacatccaag
    12001 taggtgaaga ccatattccc ttctacttgt ccaggatctt gtacatggat gtggtttgga
    12061 tcagtataca tactcatgac ttttttctgt aagacatcgg caggatcgga gatataaata
    12121 ccgtttccta acgatttact cattttgcca tttccatcga ttccaggtaa tctacccatt
    12181 ccttttggtg ggaatacgcc ttttggttcc accaagactt caccatatgt atgattgaag
    12241 ctttgtacga tttcctgtgt ctgctctagc attggttttt gatcctcacc aacaggtaca
    12301 agattcgctt tgaatgcagt gatatctgct gcttgagaaa caggatagat aaagaatcct
    12361 gtaggcacgc tttcaccgaa ttttttttgt tcgatttctg ttttgacagt cggattgcgc
    12421 cgaacgcgtc ctacgctgac taagttcaaa taatacatgg ttaattcagc taattccgga
    12481 atttgtgatt ggataaataa agtagatttt gcaggatcta atcccacggc taaatagtct
    12541 aaagcaactt gcaagacatt tgatgaaacc ttctcaggat ttttagcatt atctgtcagt
    12601 gcttgcatgt cagcaatcat gacaaacagt tggttgtctt catctgcctg catagccact
    12661 cgggttttta gagaaccgac gtaatgccct aaatgcagct tcccagttgg tcgatctccc
    12721 gttaaaataa tatttttcaa aaaagacacc tctttacgat ttgtaagtct gagcacttaa
    12781 tatcttaccg tttttcctga aaagaaacaa cagttcctaa ggaaaaaaca aggaaagagg
    12841 ataaaataac taatagatta gaaatggaag aaatatttat gggagaataa acgttctaag
    12901 aaactttaca aattactaaa ctatcagata taatgtattt tattatacat ctttaaaaga
    12961 aatcttacta aataataatg ggagtgtaat tctatggcaa tgattaaatt tgatcacgtg
    13021 gataaatatt atggcaagtt ccacgcatta aaaaatatca atctcgaatt cgagaaagga
    13081 gaagttgtcg ttgtcattgg tccttctggt tcaggaaaga gcacgatgct tcgctgtatc
    13141 aatggattag agacgatctc ttcaggtaag ctgctgatta atgatactga cttgcatgat
    13201 aaaaagacaa aactgacaga agtccgaaaa aacatcggca tggttttcca acatttcaat
    13261 ttgtacccaa ataaaactgt tctggaaaac atcaaattag caccaatcaa agtgttgaag
    13321 caagaccagg caactgcagt caaaaacgcg gagaaatttt tgaaaactgt caatatgtta
    13381 gataagaaag attcttatcc ttccatgtta tccgggggac aacagcaacg ggtggcaatc
    13441 gccagaggtt tagcgatgaa tcctgagatg ctgctgtttg atgaacctac ttctgctttg
    13501 gatccggaaa tgattggtga tgtattagac gttatgaaaa aattggctca tgacggtatg
    13561 tcaatgattg tcgtgacaca tgaaatgggc tttgccaaag aagtggcgga tcgtgttatc
    13621 ttcatggctg atggagaagt attggaagac agcagaaatg tgagagactt tttcgaaaat
    13681 ccaaatgaag tccgagcaca gcaatttatc agtaaagtta tcaatcattg ataggaggaa
    13741 aaagatgcgc aggttaaaaa aaatacgcct gattttcctt ttaggcattg cttttcttgc
    13801 actagccgga tgtaaaggaa atgggttggc aaatgaagat attttagagc gaagcaaatc
    13861 taccaatgaa atcatctggg gtgtcaagta tgatactcga ttatttggaa tgatggatat
    13921 tgagagcaga actgtacaag gatttgatgt agatattgca aaagcaatca ctaaaaagat
    13981 ccttggtgac aatggaaaaa cagaatttgt tgaagtcact tctaaaactc ggattccatt
    14041 attgaaaaac ggaaatatcg atgcaattat tgcaacgatg acgatcacag atgaacgaaa
    14101 aaaacaggtt gacttttcag atgtctattt cgatgctggc caagctttgt tggtaaagaa
    14161 aggaagccaa atcaaaagtg tagatgattt gaatgcttct acaacggttc tagcagtcaa
    14221 aggttctaca tcggctgcga atatccgaca gcatgcacca gatgcaaaaa ttttagaatt
    14281 agaaaactat gcagaagcat ttactgcttt acaatctggt cagggagacg cgatgactac
    14341 ggataatgcg attttgttag ggattgcaga tgaaaatcca gagtatgagt tagttggtgg
    14401 aacgtttact aatgagcctt acgggattgc gatcaacaaa gggcaagaaa acttcttgaa
    14461 agctgttaac caagcgctag aggaaatgca tgcagatggc acatacgata aaatctatca
    14521 aaaatggttt ccaaatgaaa ctgaaggaaa agtagagtaa gggggagagg caatgagcga
    14581 attgatacaa acttatggac caatctttgt cgacgggttc aagatcacat tgttttcgag
    14641 tgtcttagca cttgtgttta gtttgattat cggaacgtta atggcaatct tccaattatc
    14701 aaagaataaa gtagtgagcg ccttagcaaa ggcatatgta gaatttttta gaaacattcc
    14761 attattaatc atcgtgatgt ttttttatgt tgtcgtaccg atttattggt ttaatctaga
    14821 tggattccaa gcaggtacga ttggattaac gatctataca tctgctttta ttgctgaaac
    14881 agtacgttca gggattcaaa ccgtaccaaa aggtcaaaca gaagcaggac tatcttctgg
    14941 ttttacctat tcagaaatca tgcgttatat tgttttaccg caagcattca aaatcgttat
    15001 cccgccatta ggcaaccaat ttatcaacct cgtaaaaaac tcatctattt tagcaatcgt
    15061 agctggttta gatttgatgt atcaaggaga cttgatcgct agtgaaacat tcaatacctt
    15121 cgatacgtat atcatcgttg ggttatttta cttagttatc acgctaccgt tatcttactt
    15181 aatggtttat cttgagaaaa aatgggcggt tcgttcatag aaggagggga aatacatgga
    15241 ttttagcggc gctttttcat ggatgaatat ccgtttttta ttggaaggat taaaggttac
    15301 tgttgaagtt tcattgtttt cgatcatttt tagtttcttg atcggtggtc tgacaggtgt
    15361 gattcgtttt gcgaatatcc ctttcttttc caaaatcctt ggtgtagtga ttgatattat
    15421 acggaatctt ccattattat tgatcatttt cttcacttac tttgccttac ctcaaatcgg
    15481 cattcagatg aacatcttct ggtccgctgt cgcagcatta acgatttttg agtcagctat
    15541 gttgtcggaa attttccgag ctggactcaa tgctgttcca aaaggacaga tggaagcagg
    15601 attatcaacg ggactgactt atgtagaatc gatgcgaacg atcgtgttac cgcaagcatt
    15661 taaatcaatg atcccggcaa ttgtgagcca attgatttca ctgatcaaag atacttcatt
    15721 agcagtaatc atttctttac ctgaactttc tcatcaggca cgtattattt atggacagaa
    15781 caccaattat gtgcttccga tgttcatcat catgacactt atgtacttcg tggtatgtta
    15841 cgcgctttct ctgctttccg gttacttgga aaagagacgg tacagttatt aatagatgat
    15901 tcttttagcg gttgtggcaa actaaagtca caaccgcttt tcctctttcc attcgaaaaa
    15961 aagagatttc gattgtttta agcaagtaag agcacttgaa aattcacgct aaaaagtaca
    16021 caatagggag gatagaattt attgaaaaaa aggatgatac gattgacaga taatcgccct
    16081 atcggattta ttgattcagg tgtcggcggc ttgactgtag taaaagaagc cctgaaacaa
    16141 ttaccgaatg aaaatatttt atttgtagga gacacagcac gctgcccata tggccctaga
    16201 cccgcggaac aggtaatcca gtatacttgg gaaatgacgg attatctggt ggagcaagga
    16261 atcaagatgc tggtgatcgc ctgcaatacc gcaactgcgg tggctttaga agaaatcaaa
    16321 gctgctcttt ctattccagt catcggtgtg atccttcccg gtactagagc ggcagtaaaa
    16381 aaaacacaaa ataaacaagt tggcattatc ggtacgattg gtacggtaaa aagtcaagct
    16441 tatgaaaaag cactgaaaga gaaagtacca gaattgactg tgacaagtct tgcttgtccc
    16501 aaatttgttt cagttgtcga aagtaatgaa taccattcat cggtggcgaa aaaaattgtg
    16561 gcagaaacat tagctccttt aaccactaaa aaaatcgata cattgatttt gggatgcacc
    16621 cattatccat tattacgccc catcattcaa aatgtaatgg gagaaaatgt tcaactgatc
    16681 gattctggag cagaaacagt aggtgaagta tctatgctgt tagattattt caatttgagc
    16741 aattcaccgc aaaatggtcg gacattatgc cagttttata caactggctc tgccaaactt
    16801 ttcgaggaaa tagctgaaga ctggcttgga atcggacact taaatgtaga acatatcgaa
    16861 ttgggaggaa aataaggatg cgtcatgacg ggagaaaagc ccaagagctt cgcaaactgg
    16921 agcttcaaac aaatgttttc aaatatccag aaggatcggt agtgatccgt tttggtgata
    16981 caacagttat ttgttctgca acaatagaaa attctgttcc cccttttttg agagaaacag
    17041 gaacaggatg ggtgacagca gaatatagta tgctgcctcg agcaacgagt acgagaaatc
    17101 gtagagaaag cagcaaaggg aaactttctg gtagaacaat ggaaatccag cgtcttattg
    17161 gtcgttcctt gcgtgcagta gtagacttgg aaaaattagg agaacgtagt atcatcgtag
    17221 actgcgatgt gatccaagcg gatggcggta ctagaacagc aagtatcact ggggcatttg
    17281 tggcattgaa attagccatt gaaaaacttt taagagaaaa agaattgtca gaagatccaa
    17341 taaaagaaca tcttgctgca gttagcgtag gaatcttacc agatgggact tgcgtcacag
    17401 atttggatta ccaagaagat tccgcagcac ttgtagacat gaatcttgta atgacagaat
    17461 ctggaaaatt cgtggagatt caaggaactg gagaagaagc aacgtttgat ggtgaacagt
    17521 tgaatgaaat gctctttttt ggaaaaaacg cgattgaaga tttgatcaaa gagcaaaaac
    17581 atgctttgct aacagagttt gctcaaaatg atgagcggat agaagaaaca aaaacaatca
    17641 tcattgcgac aagaaatcca ggaaaagcag aagagtttcg aaatatgttc aaagaagcgg
    17701 gatatcatgt aaaaacatta cttgactatc ctgaattgcc agatgtggaa gaaactggca
    17761 gcacatttga agaaaatgcc cgattaaaag cagaaacgat tgcacaattg cttgatcagc
    17821 cagtcttagc agatgattca ggtttgaaag tggatgcttt aggcggtatg ccaggaatct
    17881 attctgcacg ttttgcaggg gaacaaaaaa gtgatgcggg gaataacgcc aaattattat
    17941 atgaattgac agatgtgccg gatgaaaaaa gaacagcaca attccattgt acgttagtat
    18001 tcgcggcacc caaaaaagac agccttgtag tagaagcgga atggcccggt cgagtagccc
    18061 gtataccatc tggcgaaaat ggctttgggt atgatccttt atttatccca gaaggtaaga
    18121 aacaaacagc agcagaatta tcgagcgaag aaaaaaacaa gatcagtcat cgggctcaag
    18181 caatgaaaaa attgagtgcc gagtggaaac aatggctgga aggagagaga tagatgcggt
    18241 atttagtagt aagtgataat catggagaca gagatatcct cgttgactta gtagatcgtt
    18301 atcgttctga agtggatatc tttttccatt gtggtgattc ggaattagaa gcttctgacc
    18361 cattatggga tacattccaa gtggttcaag gtaattgtga ttatggacca ggattcgaac
    18421 agaaaaaagt gatccaaact ggtcaggata cagtattcat gactcacggt catttatcga
    18481 atgtccgatt tggtttgaca cagttagcaa tcgaagctcg agctgcaact gccgatatgg
    18541 tgttttttgg tcatacccat caaataggct gtgagatgga tcagaaaatc ctgtttttga
    18601 atcctggtag catttctcag cctagaggcc cgatccaaat cccatcatat gctcttatcg
    18661 aaagtttaga tgaccaaatt tctgttcaat actataaccg tgcccatcag gcagttgaag
    18721 agttggcctt ttctttttcg agagagcgct gaataaaaaa actctaattt ttaataaaaa
    18781 taagctttat tttatgaatt ttgcatccgg tttcggtaaa atagtagtgt tgaatgaaat
    18841 agaaatggag gccatcagat gattggacct atagtaagag aaatgctgct ggaaaatcag
    18901 gaaacgtttt tgattcctgc agaaaatgtg gcaaatgtta tgtatcatca tccattgtcc
    18961 catggtttac ttgtgttatc gaaggtcggt tattccaaaa ttcctgtatt aggaaaagat
    19021 gatcactttg taggactagt aagtttatca gatgtggtca ataaaatgat tgacttgcaa
    19081 acaatcagca tggacccgtt agaaggacta acggttgctg atgtgacaga aacagatgtt
    19141 ccgacaattg atgaaaattg ggaactagaa gaagtactcc atttgttagt cgattcgcct
    19201 tttttaccgg ttgtcacaga agatcggatt ttcaaaggaa tcattacaag aaaagagctg
    19261 cttaaagccg taaacttcat ggtgcatgaa ttagaacgtc gtaatatatt gtctccgaaa
    19321 atcgaagaat taaaagaaca aatcgatatc gtgagctaaa gaacgagaaa ctaaaagata
    19381 atcaaaaaat aagtagaaaa aacagcgtac cagttttgac tttatcttga aaaagaggct
    19441 gggacaaaag cgtttaactc caagaaataa gaaggaattc acgaaaattg cttt
//
LOCUS       DACKAC010000051        18881 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.051, whole genome shotgun sequence.
ACCESSION   DACKAC010000051 DACKAC010000000
VERSION     DACKAC010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18881
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.051"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..435
                     /locus_tag="I0B46_001923"
     CDS             <1..435
                     /locus_tag="I0B46_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAR2084205.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(759..842)
                     /locus_tag="I0B46_001924"
     tRNA            complement(759..842)
                     /locus_tag="I0B46_001924"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(806..808),aa:Leu,seq:cag)
     gene            1370..1870
                     /locus_tag="I0B46_001925"
     CDS             1370..1870
                     /locus_tag="I0B46_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAR2084206.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(2060..2404)
                     /locus_tag="I0B46_001926"
     CDS             complement(2060..2404)
                     /locus_tag="I0B46_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAR2084207.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(2558..3478)
                     /locus_tag="I0B46_001927"
     CDS             complement(2558..3478)
                     /locus_tag="I0B46_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAR2084208.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            3665..4156
                     /locus_tag="I0B46_001928"
     CDS             3665..4156
                     /locus_tag="I0B46_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAR2084209.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(4180..6555)
                     /locus_tag="I0B46_001929"
     CDS             complement(4180..6555)
                     /locus_tag="I0B46_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAR2084210.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            6881..7507
                     /locus_tag="I0B46_001930"
     CDS             6881..7507
                     /locus_tag="I0B46_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAR2084211.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            7530..8234
                     /locus_tag="I0B46_001931"
     CDS             7530..8234
                     /locus_tag="I0B46_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAR2084212.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            8426..9259
                     /locus_tag="I0B46_001932"
     CDS             8426..9259
                     /locus_tag="I0B46_001932"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAR2084213.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            9456..11384
                     /locus_tag="I0B46_001933"
     CDS             9456..11384
                     /locus_tag="I0B46_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAR2084214.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            11522..11977
                     /locus_tag="I0B46_001934"
     CDS             11522..11977
                     /locus_tag="I0B46_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAR2084215.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            12447..12716
                     /locus_tag="I0B46_001935"
     CDS             12447..12716
                     /locus_tag="I0B46_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084216.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            13116..13370
                     /locus_tag="I0B46_001936"
     CDS             13116..13370
                     /locus_tag="I0B46_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAR2084217.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(13459..14505)
                     /gene="holA"
                     /locus_tag="I0B46_001937"
     CDS             complement(13459..14505)
                     /gene="holA"
                     /locus_tag="I0B46_001937"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAR2084218.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(14543..16816)
                     /locus_tag="I0B46_001938"
     CDS             complement(14543..16816)
                     /locus_tag="I0B46_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAR2084219.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            complement(16809..17348)
                     /locus_tag="I0B46_001939"
     CDS             complement(16809..17348)
                     /locus_tag="I0B46_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAR2084220.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            complement(17484..17702)
                     /locus_tag="I0B46_001940"
     CDS             complement(17484..17702)
                     /locus_tag="I0B46_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAR2084221.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            complement(17699..17944)
                     /locus_tag="I0B46_001941"
     CDS             complement(17699..17944)
                     /locus_tag="I0B46_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAR2084222.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            complement(18060..18788)
                     /locus_tag="I0B46_001942"
     CDS             complement(18060..18788)
                     /locus_tag="I0B46_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAR2084223.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
BASE COUNT         5798 a         3654 c         3317 g         6112 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacctg ttcaagctga acaacctgtt
      121 gtacaagaaa cggttcaaac ggaaacacaa gcagcgcctg ttgctgaaac acaacctgct
      181 cctgctgtaa cagaaactgc tgctactcca gcaagtacaa gctctgcaaa agaatggatc
      241 gcacaaaaag aatcaagcgg ttcttacaca gcaacgaacg gacgttacat cggccgttac
      301 caattagatt cttcttactt aaatggtgac tactctgctg caaaccaaga aagagtagca
      361 gaacaatatg ttacttcacg ttatggttct tgggaagcgg cgaaagcatt ctgggaagca
      421 aacggttggt actaagattc attatttgaa cgtttaaaca cattcaaaat atagtaagag
      481 gtcatgcgat ttattctttc gcatgacctc tttttgagtt taagtttagt ctttattcct
      541 taactatcgt ttgccagtat ttcttgtatc agaatctata aatctactat ttgaatcaat
      601 ctattaggtt ttaaactgtc ccaccttcac acaaattttt tttgatttat ttgaatctgg
      661 ttccgttgtt taaaattttt cctatattct tttagataat atgttatttt ctttctcatc
      721 gctccaggtg taccttctct ttataaaaaa acaaattatg cggctaagaa gactcgaact
      781 tccacgaggt caccctcact agcgcctgaa gctagcgcgt ctgccaattc cgccatagcc
      841 gcatcacatc ttttagttta ggattacatg tgttttttgt caagaacata tttttgttct
      901 ttttcatttc tgtacgccct cccttatgcc atctacacaa ataattcatc cgagcaactc
      961 catcatctat tgagggttgg tgaaaaaatc tgttttatcc atatgatcga tttcaatgtt
     1021 aaacgaacga ctctgggatt agtaactatt ttattttttt cttcatttct tttttcctct
     1081 ttttctcgat tcagctatct tctttcattt ggatattgat tatttcttct gttgattact
     1141 atccttctgg aattctagac gctgaagtaa ttcacgtttt ctttcttcta gagatcataa
     1201 gtaacgaatg aacgaacttc aaataatttt tttattgtaa ttcatatttg tattgttcga
     1261 tgattcttaa tacgatatga cacaataaat aaaaataggc atggctattt acaacaatag
     1321 tgctatagta gaaaacaaat gaataaggtt acttaaagag gtgaatacga tgaaactgat
     1381 caaactatgg gaagcagatt taaagaaagc ttatcagcta caaactagct ttggaccaaa
     1441 tgagagtgga tttatcaaca atgcttatgg ctatacattt gctgaatttt tagattatgt
     1501 aaaaaaatgc gaaaaaacgt ctttaggaat cgatcttcct gagagttacg ttcccgcaac
     1561 cgtctttatt ttagaaagtg atgaacatga gtatgtagga atttttaatt tgcgccataa
     1621 actgaacgaa acattaagaa acggcgctgg ccatattggt tacggcatct cccctaatta
     1681 tcgtaaaaaa ggatatgcca cacaaggatt agcattagta ctacaagagg caaaaaaaat
     1741 cggcattgat gaggcacttt tgtctgtaaa taaaaaaaat cttggcagtc ttaaagcaca
     1801 acaaaaaaat ggggctgcga tttatcgtga agatgagaaa gaatactata cgaaaatcaa
     1861 actgatgtaa aaaagaagga ttccaaaagg aaagggattt gaatgtcctt tatcaataga
     1921 acacatttat ttaattaact tataatatag cagacaaaat tttataaaca aagaaacctt
     1981 ttatctcttt tcttctagaa aggataaaag gcttttttgt ttttcctatt tgcttctcat
     2041 agctagtcag ccaagatttc tatcgtatcc ccaacgcaat ccgtgcataa cggctcatct
     2101 tatctgtcgt ccatgctgga taccagacta gtttgacttc tgctttcgtt acttctggca
     2161 cttcttttaa tgcgtcatgg atagattctg ttaaaatatc tgctaatgga cagcccatag
     2221 ttgttagagt catcttaatg actgtttctc cattttcagg attgaattct atatcataaa
     2281 tcaagcctaa attgacgatg tcgattccta attcaggatc aatcaccatt tccaaggcag
     2341 caagaatacg ttctttgatt agttctattt cttgtccaga ccgttcttgg tttgcttcgc
     2401 tcatcgtacg acactcctta acacgatttt tatttatcat actctttttt tagctgcttg
     2461 taaatgagaa tttagaagta agattctcgt ttaaatttat aaaaatgttc ttattagatg
     2521 taatagatta aatttattct aaaataaacc tgattcctca ttttttttgt gaacgatttt
     2581 ttatttctgg caatacgcca gttgttcgat acattttttg ttcgttttca tttgcaaacc
     2641 ggccaaatac tgaatgagtt ggtaaaatat tgataaatgc atcgtcgtcg atttcatgaa
     2701 cgatctgatc aagatcatac atttcgtagc gggtgatgac catcatgatc atgttccctt
     2761 gtttttttga atagccaccg acagacggca gtaatgtcat tccacgtacc atccgttttg
     2821 ctagttcttc tgttacctct tctggcttga cggtaacgat cattgctgtt actttttgat
     2881 gactcgtgtg gatggcatca attacttgag tcattgcata aatggaaata atcgtataca
     2941 aggcactttc ccagttaaag aagaagcctg cgatgactac aatgatacca tttagaatca
     3001 gcatgtagtt tccgactgtt tttccagttg tttgtgaaag tacaagagag atgatatcca
     3061 tcccaccagt tgtaaagccc atttttaaag ataatcctac gccaagacct aataaaacac
     3121 cgcctactag ggcattcatc aaaatattat cagtgatctg tccgactgga agcagcatcg
     3181 tcatcaccga tacacataca acattcacaa aactaaggat cgttgctcgt tttcctactt
     3241 tgagaaaccc taatacaaac acagggatat tcaaaagaaa aataaatgtt cctgtgtcta
     3301 tcttgatatg aaaccattca tataaaagcg aggaaatgat ttgagcgatc ccattcatcc
     3361 ctgcagaaaa gacattggct ggaatcaaaa aataatttaa agcgaccgca gcggtcaatc
     3421 cggttatcaa aataaccact attttcttta atctttcatt tttatgatac gcagtcataa
     3481 ttttactcac aagcacctct cccctacttt ggtattccaa atgtaagaat aacagtttat
     3541 ctattaaaat aaaatacttc gaactcaaat gagaagaatt ttagcaaaca ggtaagaatc
     3601 tgccctaatc ctatgataag atagatgtat ggtaaatatt tatatgtata tcaggagggt
     3661 tcctatggca aaagatgcta gcttcgacat tgtatcagaa gtcaatacag aagaagtaaa
     3721 aaatgcgatt cagcaaacaa caaaagaatt gtccaatcgc tttgacttca aggattctac
     3781 agtagaaatc aaattagaga acagcaatct agttgtattg agcgatgatg atttcaaaat
     3841 cgaacagatc aaagatgtat tatttggtaa gctgatcaaa agaaatgtgc caatcaaaaa
     3901 tatccatttt tccgaatcag aacacgctct aggtggcaaa gctcgacaaa ctgccgattt
     3961 ggtcaacgga atcgatcgtg aaaacgcgaa gaaaatcact acagctatca aaaatgaaaa
     4021 actgaaagta aaagcacaaa tccaagaaga tcagattaga gtgacaggta agaaccgtga
     4081 tgatctacaa gctgtgattg ctttattacg taaacttgac ttaccaatcg agttgcaatt
     4141 cactaactat cgttaatcaa aaaacgaggc tgacgtctgt cattcctcgt ttttttgatt
     4201 tactgcttct tttgataacc aaaaacgctg tattccttct tccaattccc caacacttcg
     4261 tacttctctt gctgtttgcc aatgttgtgg gaataaacgt cgataagaaa aactagaaaa
     4321 tcgttgatct gccaatacaa cgattccttg atccgccatg ccacgaatca ctcgaccacc
     4381 agcttgcaag actttgttca ttccaggaag ctgataagcg taagcaaatc caaggtgttc
     4441 cttctcatca taatatgatt tgatcagttc ctgctcatga ttcatctgcg gcaaacctac
     4501 acctacgatc atactgccga tcaatcggtc ccctctcaaa tcgattcctt ctgaaaaaat
     4561 cccgccaagt acgcaaaatc caatcaatgt ttcagcggga tttgtttgaa attttgttaa
     4621 aaatcgttcg cgttcttcct cattcatgtc cgtttcttgg attagcacat tgctgtcggg
     4681 atattgctgt ctgaatgctt ccaccgcaag atctaaatat tgataagaag gaaagaaaac
     4741 gaaataattc ccgacctttc catcagcaaa agcatgaatc gtctctacta attttgccag
     4801 actgtgttcc ctttttcgat aagtcgtctc taggtaattt gcgatgaaaa cttgctggtt
     4861 tccttctggg aaaggactcg gcagtttgta agccagactt ttttgtcccc ccagtgtttc
     4921 ctgataataa gagagtggtg aaaaactagc agaaaaaaga atactgcttt ttcctttatc
     4981 caacgactgc tcaagaaaca agcttggatc aatacaaaac tcttttacta tcagatcgcg
     5041 atacgttttt tcgactgttg tttcgtagtg atcatcataa aattcactga ccttcaaaaa
     5101 atgaagcaaa tcaaaataat aaggaagcaa ttgttcttgt gatgggtgct caggaaattc
     5161 tgccagccat tctttaattt tttcgcttaa ttgataacct gcctttacta aagattcagc
     5221 cggtgctttt tgatgatgat aatcccaatg atcttctttt gctatttctc ggatcgaatc
     5281 aaattctttt aatagtttgt tgaaacggcg atgaagtttt ttgaattcct ttggtatagc
     5341 ttccttactt cgtttcgttt tttcataaga tagttctgcc gaatacatct ctcgggaacg
     5401 attcaccaaa ttatgtgctt cgtcaatcaa aaacagatag tcctcgtttt tttcatcttc
     5461 aaaaaaacgt cttaaataaa cagttggatc aaataaataa ttgtaatccc caatgattac
     5521 gtcacaccag atactgacat ctaatgatag ttcgaaagga caaagtgtat gctttcttgc
     5581 ataagtttcg atgacttcac gagtgatctg attctcatga ttcaaaagat cccataaacc
     5641 ttcattaatc cgattgtaat aaccattggc atatgggcac tgatctggat tacaggtcgt
     5701 ctcatctaaa aagcagatct tgtctttcgc cgtcaaagtg acacttttta cttcagctcc
     5761 tgtatctttt aatgctgtca tagcatcttc agctacttgt ctagttattg ttttagccgt
     5821 tagataaaag atcttttctc cctcttcttc ccctacagct ttcaaagcgg ggaataacgt
     5881 agagattgtt tttcctgttc cggtaggtgc ttctacaaac aatttctgtt tcgtacggat
     5941 cgttttataa gcagctaccg ctagttcacg ctgacctttc ctatactcat catgggggaa
     6001 tttcaaagcc atcaaagagg cattgcgtac agtgcgccag ttttcttgaa aaacaagcca
     6061 ttctttataa tcttcagtca gctttttaaa aaaagtttct aactgctctc ttgtctgatg
     6121 ttcaaccttt ctcgtgatca cttcttctgc cgtctggaag taagtcaatt gcagatttat
     6181 ttcttgtaaa tcatgttctt gactatagat ataggcatac acccttgctt gatgaaagaa
     6241 aagttccact tgttctggtt ctaaatcttc aaatcgtggt tcggaggttt tgatttcatc
     6301 tatataataa ataccgtctc ttttgaaaat accgtctgca cggccttcaa cgatcaggta
     6361 atattcatct aattgaactg ttgtttttaa aaagacttct ttttcatagt tttctcccgc
     6421 atctttttgg agcttgcggt ggattctcgc tccttctaat gcagtatggt tacttttttt
     6481 acgattgtcg atacttccac gtcgcaaaat aaattctact aagtgccgca cagatatttt
     6541 tgactgtttg atcatttcca ccacctcttt ctctctaaga ttacctgaaa aaacctagtt
     6601 tagcaaaaag agctctcttt ccaaacataa aaaaacaccc tgctgattag gcagggatat
     6661 aggatataga atttggattt ttggatgaat ctaatgtaac aaggacatat taaaatgcta
     6721 tgaaaactta ctttccttct ctttagaata tccaaatact tttcatatag tctgataatt
     6781 cagataagta aagagaaata agtacctgtt tttgtttcct agcaatgaat gcgttatact
     6841 aatggatgaa agaagttttg gctatcaagg aggaatgcac atgcatatcg aaagattaaa
     6901 aacaggaatg attgaagaaa actgttattt ggtctacaac gaagaagcac ttttgatcat
     6961 tgaccctgga gaagatgcgg aacgaatcaa acaagaaatc aaaaaaacaa accaacagcc
     7021 ggtagcgatt ttgcttaccc atactcacta cgatcatatc ggtgcagtgg aacctttacg
     7081 cgcgtattat caaatcccag tttatgtcag cccactagag caaaaatggc taggtgatcc
     7141 gattttgaat ctttctggac tcggtagaca tgatgatatt gctgatgtta ttgtctcgcc
     7201 tgcagaatat gaatttgaaa tgaaaactta tactttagga aacatgtctt ttcgcgtcgt
     7261 ccctacccca gggcattcta taggaagcgt cagctttatt tttgatgatt ttgtcgtttc
     7321 tggcgacgcc ctgtttagag gaagtatcgg tagaaccgat ctgtatacag ggaatttaga
     7381 acaattactt catagtattc ggacccagct gtttgtatta cctgatgaat ttgcagtcta
     7441 tccaggacat ggcgatgcaa cgacgattga acaggaaaaa aggacaaatc cttttttcaa
     7501 tcgttaaaac gacttaggag gagaataaaa tgacagaaac tacactttat atcgtacgac
     7561 acggaaaaac gatgttcaat acaattgaac gagtacaagg ctggtgtgat acacccctca
     7621 caaagcaagg acaagaaggc attcattatt taggtcgcgg gttaaaaaat gtcgactttt
     7681 catttgctta ttctagtgac agtggtcgtg caatcgaaac tgcccgtatt attctaggag
     7741 aacattctaa gggaaaagaa attccttatt ttattgaaaa aaggattaga gaatggtgtt
     7801 tcgggtctct tgaaggtgga tatgacatgg aaatgtgggg tgtgatccct cgtgtattga
     7861 attttaaaac atatgatgaa atgtttacca cagaagtttc ttttgaacaa attgctaatg
     7921 ctgtatacaa cttagatacc gctgggtggg cggaaactta tgaaacattg cgagaccgtg
     7981 tatggacagg atttgaagat atcgcccatc actgcgaaaa aaatggtggt ggaaatgttt
     8041 tagtcgtatc tcatggtttg acgatcgcct ttttgcttaa tctgatcgat cctaaacagc
     8101 ctgttcgtgc tgggctttta aatggcagcg taacaaaggt cacttatgca aacggtcgat
     8161 tttctatcca cggcatcaat gatacgtctt atatcgataa cgggaaaaca catagtcgcg
     8221 actcgtttct ataatgggga tattttaatt tttaccagag aatcataaaa ggcaaagtct
     8281 gaacgagggc tttgtcttct tttttctctt cgtgtactta tctgctttat ttctcaagtt
     8341 tttcttaata ttaaaaaaca gattgggttt ctcaaaaaat aacggtatac tataagatgt
     8401 atcttaattg ggaaggaagg aatttatgtt ttttgcaaat attatcaaag cagtgattct
     8461 cggtattatt gaaggaataa cggaatggct gcccatcagt agtactggtc atttgatcct
     8521 agcagatgaa ttcatcaaac tggatgccag ttcacaattt atgtcgatgt tcaatgtcgt
     8581 tatccaatta ggggcgatct tggctgtcgt cgttctttat tttcacaagt taaacccatt
     8641 ttctccaagt aaatcaacgg tagaaaaaaa agatacgtgg acactttggt ttaaagtgat
     8701 cgttgcttgc ataccagcca tcatcattgg tttgcctttg gatgattggt tagaagcaaa
     8761 tttccacaag ttcttaccag ttgccattgt tttgatcgtt tacggtattg cctttattat
     8821 tgtcgaaaaa cgcaacaaaa acaaaacacc tcgttggact tctctgaatg actttacgta
     8881 ccaagcagct cttcttgttg ggttattcca agtcttggca cttgttcccg gaacatcccg
     8941 ttctggcgca acgatcctag gatctatctt gattggtgct tcgcgttttg tcgctacaga
     9001 gttttcattt ttcttaggga ttcctgtcat gtttggcgca agcggcttga agatcgtcaa
     9061 atatttagct gaagggaatt cattcaaaat ggaagaaact gttgttcttc ttgtaggaac
     9121 attcgtatct tttgtggttt ccgtcttagc tatcaaattc ttactaagct atttgaagaa
     9181 aaacgacttt accgcttttg gctggtatcg tattatcctt ggtatcattt tgattggtta
     9241 ttggtttatt gcaatgtaga aaaaaccaga acacttctct tatttgagaa atgttctggt
     9301 tttttatttt ctttaataat aatcattata aataataaaa acaacatcaa gcttaatcac
     9361 caaatgataa ccattatcgt tctcatttag ctgttattat tatcaattac atttttatta
     9421 tgctatcctt gttttgacaa ggaggtctaa taacgatgac taatcagaaa aaagcttttg
     9481 cagcaacaat cggctgcctt ttatttatga tcttcggttt tatcttcgac cgattctctc
     9541 ttaccatcac cccacttttt tatatcagtg ccattatcat cggtggatat aaacaaacat
     9601 cggaaggctt gaaagaattg tggaatgatc ggacactgaa tgtggattta ttgatggctt
     9661 tagctgctat tggtgcctgt ttgatcggta attactttga aggagcaatg ttgacattca
     9721 tcttttgttt aagcggtgca ttagaggaat acacaactaa taaaagtcaa aaagaaatta
     9781 ctgctttgat gaatcttcaa ccacaaaaag cccaattatt aaaagaaaat ggacaaatgc
     9841 aagaagtaga tgtttctcaa ctaaaggtgg ataacttggt atttgtcgct aaaggagcag
     9901 ctgttccgat agatggcttt ttagaaagca cacaggcaac gatcgatgag tctgcattaa
     9961 gcggtgaatc tgtgcctgta gaaaaatatt taggcgatga gatgtatgca ggaacgctga
    10021 atcaaggaaa tcccatcacg attcgagtaa caaaaacgag ttccgaaact gtctttgcta
    10081 agattatcca attagtagaa gaagcgcaga atacgccaac tcaaacagct tcatttatcg
    10141 aacgaatcga aaataattat gtaaaattga ttttgcttgc tgttcctcta atgatccttc
    10201 tcccccactt tttcctaggt tggagttggg atgaaagctt ttatcgagga atggtattat
    10261 tagttgtggc ttctccttgt gcgcttgtcg catctgctac accagcttct ctagctgctc
    10321 tttcaaattc tgcaaaaaat gggattttaa tcaaaggcgg tatccattta gaaaagcttt
    10381 ctgaattaaa agcgattgcc tttgataaaa caggaacgct cactaaagga aaaccagttg
    10441 taactgacag ctttttcttt gatgaaaagg atttggcaca acaacttctt gttgctatgg
    10501 aacaaaaaac gacgcatcca cttgctcatg cgattttgca atatttcgat tgcacaatac
    10561 ctgaagaaat ccaatactta ccaatagaag aaattactgg ttttggatta caaactacct
    10621 atttaggtgc gcaatggaaa gttggcaagc acgcatatga tcctgagacg atgataatca
    10681 gcaaagagat tgcggaaatg atcgaacgtt tggaaaatca aggcaaaacc gtgatttatc
    10741 tttctaaaga tcagcagtta attgctgttt taggtctgct agatattcca aaagcaaata
    10801 cccaacaagt aatttcctac tttaaatccc aaaacatcca tacaagtatg ataaccggcg
    10861 accattctgg cacagcaaag gccattgctg aacaagtagg gattgaacat tactatgcaa
    10921 gctgtactcc ggaagaaaaa acacaactag tcaaaaaaga gaatgaactt taccaagtaa
    10981 atgcgatggt tggtgatgga gtcaatgatg cgcctgcttt agcagccgct tctttaggtg
    11041 tcgctatggg gcaagggaca gatgccgcta tggatatcgc agacatcgtc ttaatgaaaa
    11101 gcgacttaaa taaatttgtc tacagtcatc ggctatcttt aaaaatgcgg cggatcatca
    11161 aacaaaatat tattttttcg attcttgtta ttctcttgtt gattgcttct aacttcctgc
    11221 aatttttaaa tctacctctt ggagttgtag gacacgaagg aagcacaata ttggttattc
    11281 tgaatggctt gcgtttacta agacctttac ctgatctttc tgattcttca aaaaaatgta
    11341 agtcttgtcc actatacaaa gcgcggctgt cttctgtcaa ataagcaaag tgttttttta
    11401 tgattttgtt tattttatga aaaaaacagc ttatgaatag gtcttgaagg atgaattcct
    11461 acttttgtaa ctttctaaaa acatgttatg attgtttcga agaaagtgag gaatatattt
    11521 catggaaagt tggctttttt taggaattat tttacttgta gcaattttag ctaaaaatca
    11581 aagtctgatg atagcggcag cttttgtttt gattatgaag ctcataccag cctcccgttc
    11641 ctttttccct tggcttcaag gcaaaggaat caatattggg gtgacgatca tcacagcggc
    11701 tattctaatc cctatcgcaa caggagaaat cggtctacgt gatctattag aagcctttaa
    11761 atcccctatt ggctgggtag ctgttttttg cggtgggcta gtggctgtct tatcttccaa
    11821 gggtgtagga ttgattgctt ctgatccaca aatcactgtt gcacttgttt tcggcacgat
    11881 tttaggtgtt gtcttcttaa gaggaatcgc cgccggaccg gtcattgctt ctggtatcac
    11941 ttactgtatc ttacaagtat ttgctttagg aaaatagaac aaattttgtc tattttagga
    12001 gattcttaac acccccaatt tactgatccg tgatatactg acaacgaggt gatacaatgt
    12061 tcgaaaaatt aaagtatttc ataggaacaa tcggctggtt tcttctagcg gtacttttga
    12121 ttctgggctt agcttatcta ttatttcctg atttgataca aaatgtgttt gatactgctt
    12181 tttgattgac ctattaaaac gatatagggg atgctcctgc gatgcagggg ctttttttaa
    12241 tcaatgtttt gtaataattt aggccgtgcc aatgagactt cctatagaat tcgatgctct
    12301 tttttctttt tggctcttct gctctttttt gttatactag attacagtgg acaaaagaaa
    12361 gaatgatcgc atccacttca acgaatatca aaatggaaga attagtgcct gttttcaggt
    12421 tactttgaaa agaaaggaag acgcttgtgg aaacttttga aggtctttta attgatacgt
    12481 acgttttaca agaacagcca atgatgctga ctgggatcaa cttgcagctt gaggaccgtg
    12541 tgatcaatgg cctgcttaaa gatcgctatt tggcaaaata tatcttacgt ttgcccctaa
    12601 ataaatatgt gctggaagta agtggcaaat ggaaccgtcg gaaccagttg gtcataaaaa
    12661 aaatgttgat aaaaaatatc gatgaataca ttaaaataat cggtacacca gaatagcctt
    12721 tactgatcaa aggatactgg ttataaaata actgcagttt cttaatagaa aaacaaagaa
    12781 caacagggaa tcgtatccct tttcaaaaaa aactttgcaa acggttctct ttgttatata
    12841 ataacttagt accaaacatt cccattcaaa ctttgtttaa atagataaat aaaaatctag
    12901 tttggtttga cgctattgcc tcccacaata gcgtcttttt gatttttttc agcttgtact
    12961 atccgttttt actgaaaaaa aatcctaata aaagactgtt aaggaaaaaa acttgtcatg
    13021 ttcatattgc aaagcacgct gcctttaggt ataataatga atgttgaatt atgcggtaac
    13081 acgcgtaaga acttaagtta tcggaggtga aactaatgcc aaacattgaa tctgcaatca
    13141 aacgtgttcg tacaagcgaa aacgctaatg tgaaaaattc atctcaaaca agcgctatgc
    13201 gtactgcaat caaaaaattt gaagatgctg tagcttcagg tgctgacaac gtagatgcgt
    13261 tatataaaga agctgttaaa gctattgata tggctgaatc aaaaggtcta atccacaaaa
    13321 acaaagctaa ccgcgacaaa tctcgtttaa gcaaaaaaat cgctaaataa aagaatcggc
    13381 ttcggccgat ttttttattt gtcaaaaaaa ggacaaaaac aaacgacctt cccgcatgct
    13441 ttgccctcgt ctttcttctt atcgatttct taacaactgc cctgtgcgca aaacgaatag
    13501 ttgaaaactt aattcttttt cgatttgtcc cgttttgatt tgataatcca tctcaactaa
    13561 ttgatcaaac aactgtacca gcaaatgttc atcgaatttt tttacttcct gcatggcaag
    13621 ttttacacga tatggatgga ttttcaatgt ttcagcaata tttgcctgct gataaccaat
    13681 ctttacaaga aattttgtct gtaaaaataa acgtaattga gaaagcaaaa tcgcattaat
    13741 tttgattgtt tcttcccctt gcataactaa atcttcgtat aaacgcaaag cttgttccgt
    13801 cttccctttt aaaatatatt gggtcatgtc aaaaatatta tgttctaacg ttttaggaac
    13861 taattcttct acttctctta ccgtgatttg ctgttgattt tgtccataaa ggaccaattt
    13921 ctgtaactct ctcatcattt tggataaatc taagtcggtc aacctcaaga ataaatcaat
    13981 tgcacttcgt tccattttga ttgaggaatt ctctaaggta ttcatcagat attgacgtac
    14041 ttctttttca ttcaactgtt ggacattgat gataattgct gtttttttca attgtttcgt
    14101 tacttttttc cgttcatcca acttttcata tggcgcaaag aatactaata cagtcgattc
    14161 taatggttct ttcaaataag ccgtcaataa atcagtattt tgttcgattc cattgtttac
    14221 tttttcacct gttaaaaaat aaggattctc tacgaatact aaccgttgat ctccgaaaaa
    14281 cggcaacgtc tctgcttcag ctactacctc atctaaactt gcattttcca tgtcaaaact
    14341 taaaaaattt aagtcatctt cgccctctaa ttggattttt ttgacaattt ctgtccgaac
    14401 ttgatcttgg agaaactttt ctgttcctaa aatcaagtaa caagatgcaa gtttttcttt
    14461 acgtattttt tgtaactctg cttgtatact caaattactc accactttca aaaattatcc
    14521 taccatgagc agatgagaat tactagtcta tcattgtttc cactagccca tgaggatgaa
    14581 aaactggatg ccagcgatag gtgatcattc cttgttgatc cgttcgccat gtttttatct
    14641 gattttcttc tagtgtctcc aatacttcgc tgtgcgggtg accataacga ttatttacgc
    14701 cgcaggatat aatcccatgt tttgcttcaa tatgggaaag aaaatctgtg ttacttgacg
    14761 tacggctacc gtgatgacct aatttgacta catcggcttt cagattagga taaatcaaaa
    14821 gcaattgttt ttccattgct tgattcaaat ctcctaaaaa taaaaagcgc ctattgttat
    14881 attccatcca tagaccaatc gaatcttcat ttcttccctc tcccttttca aaaggagtca
    14941 atacataaag atcataataa ccgctgatcc tgtccccatg tttgatttca atgatctcag
    15001 taccattttt ttgagcttca gataaatgcc tttgtaatgg ctcatttcgt ccagcgcccc
    15061 aaccgatata aatttttttg atagtaaaat gctgagataa cgtgggtaat tctcccacat
    15121 gatccgtatc gtcgtgtgtc aagatgagtt tatcgattac acttatcccc tgacctttta
    15181 gatatggaac gatatttctt tcagcaggag gacgtatcat accttgcgcc cacttttctc
    15241 tttcaaagtg taactgacct ccagtatcga tcaaaatcgt ttcttttcga aaaggacttt
    15301 gcaaaaagat actgtctcct tgaccgacat cgataaaagc gatcgttgaa gaaaagcgta
    15361 aaaatggcaa agtcaaaaaa ataaaagaaa aaagaccaac ataaaacaag tgtttcacag
    15421 gttgttcttt caacctatcc atacaaatca gtaatccgaa aatatacatc ccagatagca
    15481 caaggggcaa acgtcctata atgagagacg accctgaaga aaaatcgatt attcgctcaa
    15541 ataagattaa tccctcttct atcagcaaaa gtaaagtttc cggcagccag cgattgaaga
    15601 gtgtgagaaa taacacacca ggaagaaaaa gaaacttgaa taccggtgca atcagtaatg
    15661 taaaaagact acctaaaata ggccactcat aaaaatgcca tacaatcaac ggcatcgtga
    15721 tcatagtaaa caggacgtga gaggccgttt ttttcaacca atctccatca aaagaaatca
    15781 ggataaacaa aaaactcatg aaaaaactaa gctgtccacc cgcatgaaaa agaaatcgag
    15841 gatcaaatag taaaagaagc caaaagacaa ctgcaaataa gtccatggtg gaataatgct
    15901 tattcagcaa tttgactagc aatcgcaata aaaacagcaa actagcacgc aaaacagaaa
    15961 tagaacttcc tgcaatagaa acataacaga tagttagtag actcaacgga ataatactga
    16021 cgtgtagtgg ccatccgata cgtcgaaata aataatacag ccaccctaaa taaaattgga
    16081 tgtgtagacc cgatagactg aacagatgca gcaaaccact ctccctaaaa atttcttctg
    16141 gttctctgaa tcctgcatct ttatagcccc ataataaaga attaatatat acacttgttt
    16201 tttgcggaaa ttcagattgg acaaaggaga taaaagatcc tctttttctc tgaagccagt
    16261 ttcgccagtg attttgcatg gtgatctgtt caatcttttg aatttggaac cgtccagaaa
    16321 ttctatggtt cctgaagtaa gcttcttggt caaaattata aagattccgc ttttttgctg
    16381 gagagatgaa ctcgccttca gcagtcaatt ctattccctt tcctcggaag ttaagccatg
    16441 ttttcaattc tgcttctgtg tttacttgat aagaaagctg gagcttttgt ttattctctg
    16501 ataaccttcc ttccattgtc aaaaaattac catttgcacg aatcgtatct gtaaatacag
    16561 taatttgcgc agatgttgtt ttttcctcta aaaatgaata ctcttgtagc ctataagaaa
    16621 tggataccgt tatgctacac aaaatacaca agtacgcgat aaaacgtcct tcttttagcg
    16681 aaaaccataa aaaaattccg gcaagaagca aacgataacc ccaattgtca gtgtaataca
    16741 acaaacagat aatcatcgac gtaattgccg gaaaaatcaa ccgatttcga acatgccttt
    16801 ctaaaacgct agacatgttt tcctgttaat tcattttgaa gttttgaaaa atgctgtgag
    16861 tctaaagcaa cttggtgtac agatacacct aattgttcga tcaacgcttg ggcataagga
    16921 tcatttcgat agtcatggag ataattgatc cctttcacac cagcttgcaa caaagccttt
    16981 gtacaggcca aacatggaaa atgtgtgaca tagatttctg aaccttccgt cgggatacca
    17041 agttttgcac actgtaggag tgcattcatc tctgcatgga tcgttcgcaa gcagtgtcca
    17101 tccacgacgt aacacccttc atctatgcag tggacatctc cactgactgc tccattatag
    17161 ccgccagcaa tgatcctttt ttctcgaacg agtgtcgctc ctacttctaa acgtgtacac
    17221 gtactacgta aagataacag tactgcctga gccataaagt actggtccca aggaatgcgt
    17281 tcattatgtg acttatcagt aactttttct ttattcgtat cgcatgcttt tgaagatttt
    17341 tctaacatgt tttatattcc tccgtttttt caagttgatt aaaaagtact cattgcttaa
    17401 acaaaatatt ctttaaatat aacgaatcat cctgtctgac aaaagatcct acccttcact
    17461 gccgaaatga agtccgtcat ccattagccc attataaaaa ttcttcgaat ccatttgttc
    17521 aagaaattcc gtataaaaat cgatacttca caatcatggc gttttgctac ttctgtgaga
    17581 acagagatat attctttgat caacttgtta ttccgatatt tttgttttgt ctcatcgact
    17641 gctggtggcg tgataaagaa agcatgatca gaactatagt cttttttcag ctgaactatc
    17701 atccactgca gattttttgg tacgtttctt tagaaagttg tttatgctct gctagatcat
    17761 tcgtcccaat gagaataaat actttatctg ctgtatagct aagtacatct ttggcaaagc
    17821 gttctcgtag agcttgactg ttattgccgc tgacagccgt attcataata tgaatcccag
    17881 atattttatt ttggagatag acattcagca ttggtttttt taatgtttct ttgcgaacaa
    17941 tcaagctatt tcctatccaa aaatactacc tcctcgtcct gccaagctct tagaattgga
    18001 ttattcttag tatccattag cgtccattta ttgctcgtgc cagtcgattt tgaatcattc
    18061 tatttaattg taacgaagtt ttttagtttt tcaactgtct tttcaccgat accagaaatc
    18121 tcctgcaggt cttccacttt ctggaaggat ccgttttctt ctctaaatcg aatgatttct
    18181 tgcgctttga ctgatccgat cccaggaatc tgctgaagtt ctgatggagt ggctgtattg
    18241 atatttattt tgggaggctc ggaatctgct gtaggctgtt ttccttccga ggatgccaac
    18301 tgatcaagat gttctggcag ctcttcacct acagcaggga tatataggac ttgctgatct
    18361 tctatcttcg cagaaaagtt gattcgatcg ctatccgctt cttctaataa tccacctgca
    18421 cgctttagta catcatagac ccgttcttct gaagaaaagg aatagatacc aggctccttc
    18481 acacttcctt tgatatcggt gtacatgacc tttggacgag catcctcttc tacatcgttt
    18541 ccttcagctg atggatcttc tccactggaa ctaaacgaag aaagtatttc ttttgcagca
    18601 gtctcttctg tttctctata aaatagcaaa tttgataata aaaaaccact tgcgatacaa
    18661 aagataagaa ctatcataga catccaaatg atcaatttgg gtgaagctag tattttcttg
    18721 ctgatttttt ctatcattgt tcttttttca aagaatgacc agtaaaaatc attcattact
    18781 cctttcattt aaaattgaag aaacagtgag ctctggcaaa atcgtccctt caacatctaa
    18841 ttacgtcaat tttttctttt gtttttattt ttatcgtgaa g
//
LOCUS       DACKAC010000052        18844 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.052, whole genome shotgun sequence.
ACCESSION   DACKAC010000052 DACKAC010000000
VERSION     DACKAC010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18844
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.052"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(565..1131)
                     /locus_tag="I0B46_001943"
     CDS             complement(565..1131)
                     /locus_tag="I0B46_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAR2084224.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1131..2087)
                     /locus_tag="I0B46_001944"
     CDS             complement(1131..2087)
                     /locus_tag="I0B46_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAR2084225.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2087..2743)
                     /locus_tag="I0B46_001945"
     CDS             complement(2087..2743)
                     /locus_tag="I0B46_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAR2084226.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3113..5131
                     /locus_tag="I0B46_001946"
     CDS             3113..5131
                     /locus_tag="I0B46_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAR2084227.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            5558..7972
                     /locus_tag="I0B46_001947"
     CDS             5558..7972
                     /locus_tag="I0B46_001947"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAR2084228.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8320..9873
                     /locus_tag="I0B46_001948"
     CDS             8320..9873
                     /locus_tag="I0B46_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAR2084229.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10254..12038)
                     /locus_tag="I0B46_001949"
     CDS             complement(10254..12038)
                     /locus_tag="I0B46_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAR2084230.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12053..13774)
                     /locus_tag="I0B46_001950"
     CDS             complement(12053..13774)
                     /locus_tag="I0B46_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAR2084231.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13874..14635
                     /locus_tag="I0B46_001951"
     CDS             13874..14635
                     /locus_tag="I0B46_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAR2084232.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            15602..15870
                     /locus_tag="I0B46_001952"
                     /pseudo
     CDS             15602..15870
                     /locus_tag="I0B46_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            16542..>18844
                     /locus_tag="I0B46_001953"
     CDS             16542..>18844
                     /locus_tag="I0B46_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084233.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLTTGEDGKLAIDGLAPGAYQLVET"
BASE COUNT         6198 a         3344 c         3790 g         5512 t
ORIGIN      
        1 tttattttcg gaaaaagcta atatagctaa gattgaaaca tccatctgga atttatcttt
       61 tttactgctt tgatcataaa agtctgcata ttcatcactg atattttttc taataaattc
      121 ttccatcata aaaatcacct caaaacgatt atacgaactt acgttcttgt tgtaaagcga
      181 tatttgaggg gcaaaaaagg ggcataattt gactgatatt aagttagcta ttgatatatg
      241 aatgacatac aaaaatataa aatcttttaa aaatgcttat atatcaatgt ttttgccatt
      301 cttttatata tctgttatat gtcaaaaaaa tgtgttcaat ttcaatttgt atttgaataa
      361 agaggttgtg aaaaagctgt cctgcatcaa gtaataagaa cagccaatca aaaacagctt
      421 ctcatgtttt tgatttggtt ggacttatta ccgcagatat aagcttttga gcaccgtgaa
      481 taaagaggtt atgaaatcaa cgctctaacc tgagtattag agaaaaaaac gcaaaacata
      541 tcatttatgt tttgcgtttt ttatttatct ttttttctca atacaatata aaataggcgg
      601 ctggttcttt tggttgataa accgataatt cagcacatta tatgtttcct gtggcagttt
      661 ggaacaaaaa gaatcaacca tatccagttc tttttctcca ccttcatgac catagtagac
      721 gacaaggatc aatcgtccgc gaggcacaag tcgtttcaat atttcttcca tagctgtgcg
      781 tgtagtctct ggcatagtga tgattgcttt atcacttttt gggagatacc ctagattgaa
      841 aatccctgct ttgagatttt gttgttcagc tatcacttct cctaacgttt cgtggccttg
      901 tagaaacaat gagacacgtt cttctaactg acgctgttct agcctttcac gagtagcatc
      961 gattgcttgt tgctggatat caaaactata gacatgacca gtttttccga tgtgttctgc
     1021 tagaaaaacc gtatcgtggc cattgcccat cgttgcatct acgacgtgat cccctggttg
     1081 caagatttct tgcagtaatg tatggctgaa atgcaaagca gtttgcaaca tcagacgctc
     1141 acctttttcc gtacatcata ttttccttgg aagctatctc tgcgaagaag ttcttcatca
     1201 attgcattca agacttccca ttttttcaag ctccacattg gtccaatcaa ggaatcccac
     1261 ggcgcatccc ctgtcaaacg atggatgatg atctcttttg gtatcatttc cagctggtcg
     1321 caaatgacat tgacatagtc aggacggctc attaattgaa ggcgaccttc atgatagtcc
     1381 cgcaacattc tcgttttgcg catcagatgc atcaaatgga gcttgattcc ttggatatct
     1441 gaatctaaaa tggtgcgtct gacattttct ttcatcattt ctaaactttc gcctggtaat
     1501 ccgttgatca aatgcgtaca aacacggata ttatgtttgc gtaatttagc cacgccatct
     1561 aaataagttt gataatcatg tgctcggttg atcagttttg aagtttcttc aaaagttgtt
     1621 tgcaatccaa gttctaccca taaatacatc cgctcgttca gttcagcaag gtaatcaact
     1681 acttcatctg gcagacaatc cggtcgtgta ccgactgata atcctacgac acctggtaaa
     1741 ttgactactt gctcaaatcg ttctttgatt atttcaactg gcgcatgggt gtttgtaaaa
     1801 ttttggaaat aaacgatgta ttgttggaca tgaggccatt tttgatgcat catgtcgatt
     1861 tctttctgga attggattgg aagagggtct tcaggtgcaa cgatcatgtc tcccgaacca
     1921 gatacactgc aaaaagtaca accgccatgg gcaacagtcc catctcgatt cggacagtca
     1981 aatcctccat cgatcggcac cttgaaaatt ttcccgccaa actcatttcg taaagcgtaa
     2041 ttccaagaat gatatctttt atttccttct gaataaggaa aagtcattaa aagtccttct
     2101 ttctgtaaga gttatctatt tgttttcttt tttctctata cactggataa gtcaggcaca
     2161 gccaagaaag actgagtaag tagccgccga tgatatcagt aggaaaatga acgcccagat
     2221 aaatgcggct tatcccgaca cttactataa taaacatcat cagcaattga agcaatgtgc
     2281 gtaaggttct tttttgaaca aaagctggaa gcagaaatag taaagaaccg taaagaatca
     2341 tactgcctgc tgcatgtcca ctagggaaac tgtaacttgt ttcaactact aagtgcgcca
     2401 atgaaggacg ctctcgcatg aaaaatagtt tgatcaccgg gttgatgatt ccagcaatta
     2461 caataaatcc agaaacgaac caaaatgttt ctgccctttt tttcttatac cacatccata
     2521 aagccgcaca agcaaataaa atgatgacgg taagaggatt accgaattta gtcataaaga
     2581 gttggtagct gttcagatca ggataagaag ctctgacaat gcctgtaaac cactgatcga
     2641 aaccagaaag ccacgacaga tggaacttta cgatccagcc caaaatgaaa aacaggacaa
     2701 gaaagaagct accagccacc tgaaatgatg ttttatattt catacaattc ctcctttgaa
     2761 gcaatgactt attatagcat ttttggtcca gcgaaaaaaa cggtaaagag ataaaaccaa
     2821 tgtatgattc ctctttgaat aaccaatatt cttataaaaa aaataactgt aatcggaaag
     2881 acattttttg gtaatgcaaa tgaaatgtta gggaaataaa atcagtgttt ttcgaaatcc
     2941 ttttaaacaa gaacgtagac gtaggaagaa cttgaaaatg aacagaagtt gttcattacg
     3001 gtaaattaca atatgattac aaaaaacaac aaagctctta aataactttt aataaatatt
     3061 aagagaagtt ataattatta ataatagata aattgaagtt ggaggataga agatggaaaa
     3121 cattgcaaga aaagaacgga gacgtttaga acagaccaaa cgttttcgaa aagcaaaacg
     3181 aggggcagca atcgtgggaa ctgcgatggt aggctgttct gtcgctgccc cattagtaca
     3241 gccggttcct gttcaagcgg atgaaacacc tgttcagttc ggcgcaagaa tcaatacagc
     3301 ggcatttatc acagaaattg ctacttatgc tcaaccaatc gctcaagcca acgatctata
     3361 tgcatcagtg atgatcgcac aagcagttgt agaaagcggt tggggcagta gtgcgttatc
     3421 tcaagcacct tattataatc tttttgggat aaaaggcagc tatcaaggac aaaccgtcta
     3481 catggatacg cttgaatttt taaataatca atgggtaaat aaaaaggagc ctttccgaca
     3541 atatccttct tttgctgaat ctttcaatga caatgcctat gtacttagaa atacttcatt
     3601 tggcaacggt tattactatg ccggtacttg gaagagcaac acgaaatcct atacagatgc
     3661 gacagcttgt ttgacagggc gttatgcgac tgatcctggt tacgcaggca aattaaataa
     3721 tatcatcgca acttacggat tgacccaata tgatacacca gctagcggaa acgcaggcgg
     3781 tggagcgact acaggtggca ataatactgg aagtgcaggt agtactggcg gttcaagcaa
     3841 ttctggtaat acaaactcag gttcagcaac ttcgaccact tatactgtcc aatctggtga
     3901 ttctgtttgg ggaatctcaa ataaattcgg tatcacaatg gatcaattga tccaatggaa
     3961 caacatccaa aacaatttca tttatccagg acaaaaactg acaatcaaag gtagccagga
     4021 aaatggctct tcgacgaata attccggtaa caataccaac tcttcaggaa atgctggtac
     4081 atcaaacgga ggacaaacaa ctggtgcgaa atacactgtt caatctggtg attctgtttg
     4141 gaagatctca aatgatcacg gaattacgat ggctcaattg attgaatgga acaatatcaa
     4201 gaacaatttc gtttatccag gacaacagct aatcgtatca aatggcggtt cttctaatgc
     4261 tggagcaaat actggctcaa atactgggaa tacaagtggc tctagttcgt ccagctcaag
     4321 ttcttctaat acagcttcag gaacgaaata tacagttaaa gccggagact ctgtttggag
     4381 cgtctcaaat aaatacggaa ttacgatggc tcagctaatc caatggaaca atatccaaaa
     4441 taactttatc tacccaggac aacaactgac tgtttcaaac agtggatcaa caaacagcac
     4501 gtcaggtaat acaggttcgt caaccaatac aaataacagc agttcagtta atactggcag
     4561 cagttcgtct caaggcactt ataccgtgaa agcaggtgaa tctgtttgga gcgtggcaaa
     4621 taaaaacggc atcacgatgg atcagttaat cgaatggaat aatatcaaaa acaacttcat
     4681 ttatccagga cagcaattga tcgttaaagg tggaacttcg gcaaatacaa atacaggttc
     4741 aactacttct gcagcgaaac caaatacacc gaatacgtct gctacgacaa gtacatcatc
     4801 cactggtaat acgatgtata ccgtgaaagc gggcgagtca gtttggagcg tagcgaacaa
     4861 acatcatatc acgatggatc agttaatcga atggaataat atcaaaaata atttcatcta
     4921 tccaggacag caagtgattg tgaagaaagg ttcatctcaa tcaacaaacc aacaatcagt
     4981 tactggttcg aaaacctata ctgtgaaatc tggagaatct gtttggagtg ttgctgacag
     5041 tcatggaatt acaatggctc aactaatcga atggaacaat attaagaaca actttattta
     5101 tccaggtcaa acgttgattg tagctaaata aaatagctgt tcgaagaaat aacgattgaa
     5161 ttccacaaaa atatttgtta ctataaggct acttaaatat gacaacactt gaaaaggaag
     5221 tagtaaaaag actgaagttt cagagagcgc atggctggtg agaatgtgca cggatgcttt
     5281 tgaactcgcc tttgagttgc ccttgctgaa tacccgagta agcaagcggc ggtgacggcc
     5341 gttacaacgt tcgaggcctt ttcactaagg taaaattagg tggtaccgcg tagattacgt
     5401 cctataaaaa gagcaatctt tttataggat ttttttattt cttttgtgaa gtttgagaaa
     5461 acaaagcaaa aggaaataaa aataaacaga ttctgccggt tttttcgtta tatttataat
     5521 agaaacaagt gttttcaata gaacaggagg acagcaaatg agttacaatc ataaagaaat
     5581 tgagaaaaaa tggcaaaaat actgggcaaa aacgaatact ttcaacaccc atgatgatcc
     5641 tgaaaagcca aaattttacg cactggatat gtttccctat ccatccggtc aagggctaca
     5701 tgtagggcat cctgaaggtt atacggcaac ggatatcctt tcacgtgtga aaagaagtca
     5761 aggatacaac gtgctgcatc caatggggtg ggatgcattc ggtctgcctg ctgaacaata
     5821 tgcgctagat acaggaaacg atccagctga gtttacccaa aaaaatatcg aaacattccg
     5881 acgccaaatc aattctttag gatttagtta tgactggaat cgcgaagtga atacaactga
     5941 tccggagtat tataaatgga cacaatggat ctttacgaag ctttatgaaa aaggtctagc
     6001 ttatgaagca gaagtagcgg taaactgggt acctgaatta ggaacagtta tttcaaatga
     6061 agaagtgatc gatgggaaaa gtgaacgtgg tggctatgat gtagtcagaa aaccaatgcg
     6121 ccaatggatg ctgaaaatca ctgcttatgc tgatcgctta ttggatgatc tagaattagt
     6181 tgattggcca gaaagcatta aagaaatgca aagaaattgg atcggtcgtt cagtaggagc
     6241 aaatattgaa tttaaagtgg ctggtacgga taaatcctat accgtattta cgacacgacc
     6301 agatactcta ttcggtgcaa cttactcggt tcttgcacct gaactagatc ttgttagaga
     6361 aattacgacc cctgaacaaa aagctgctgt agaagcttat atcgaagaga cagctaaaaa
     6421 atcagatcta aagagaacgg atcttgcaaa agaaaaaaca ggagtattta ctggtgctta
     6481 tgcaatcaat ccagttaatg gcaaggaaat cccaatctgg atcgccgatt atgtattatc
     6541 ttcatacggc acaggagcca tcatggctgt tccagcacat gatgaacgtg actttgaatt
     6601 cgctcaaact ttcggactag aaattcttcc agttatcgaa ggtggagatg ttgaaaaagc
     6661 tgcttacaca gaagatggta cgcacatcaa ctctgaattt ctagacggca tgaacaaaca
     6721 agaagcaatc gataaaatga atgaatggct agaagaaaat ggcgtaggga aaaaagaagt
     6781 cagctatcgc ttacgtgact ggctattctc ccgtcaacgt tactggggtg aaccaatccc
     6841 aatcatccat tgggaggacg gaacagtcac agctgtccca gaagaagagc taccattacg
     6901 gttgcctaag acaaaaaaca tcaagccaag cggtactgga gaatctccat tagcaaatat
     6961 cgaggagtgg gtcaatgtcg ttgatcctgt aacaggtaaa aaaggccgtc gcgaaacgaa
     7021 tacaatgcca caatgggcag gaagctcatg gtattacctg cgttatatcg accctcataa
     7081 caaaaaagaa ctagcagatt atgaaaagtt gaagcgttgg ctaccagttg acatctacat
     7141 tggtggagca gaacatgcgg tacttcattt actatatgca cgattctggc ataaattctt
     7201 atacgatatc ggtgttgttc ctacaaaaga accattccaa aaactataca atcaaggaat
     7261 gatcttaggc gaaaacaacg aaaaaatgtc aaaatctcgt ggtaatgtag tcaatcctga
     7321 tgatgtggtc gaaacttacg gtgcagatac actgcgcatg tacgaaatgt tcatggggcc
     7381 attagatgct tcgattgctt ggagcgaaaa tggcttagaa ggaagccgta aattcttgga
     7441 tcgtgtatgg cgtttgatcg ttgatgagaa caataaaatg cgtgaccgta tcactacatt
     7501 gaatgatggc aagcttgata aagtgtatca tcaaacagtg aaaaaagtga cagaagacta
     7561 tgaaaatctt catttcaata cagcaatctc ccaattgatg gtctttatca atgaagccta
     7621 taaagtggac gctctgcctt atgaatatat cgaaggattt gttcaactac tcgcaccgat
     7681 tgctcctcac atgggtgaag aactatgggc aatccttgga aatgatggtg gtatttctta
     7741 cgcaccatgg ccaacatacg atgaagcagc acttgttgaa gacgaagtag aagttgtttt
     7801 ccaagtaaac ggtaaagttc gtgcgaaaag caatgttccc cgagacttag gaaaagatga
     7861 gctagaaaaa gcagcattag ccaatgaaat agtgcaagaa tatattgaag gcaaaacagt
     7921 cagaaaagtt attgcggtac caaacaagtt agtcaatatc gttgctaatt aattggctct
     7981 ttgtcaataa ggactgatga gttgtgcaaa atcaaatctg ggccagaatg aacttcattc
     8041 tgtctcagat tttttgttat ttcaaattga ttaattgatt gatcaaaaag gttttgattt
     8101 tcatgttctc aaacacgttc aatgtctttt tcttctgaat ggtcttgtaa gagatctctg
     8161 taatcatcaa atttttgtct attaatctta gtattttttg atgtataaac tacctgaaat
     8221 tgagagtaca taccaaattc ataaattttt tattagtggg actagtgatt gtatggattt
     8281 tgacgcataa tgaatgaagt atgagaaaag gagagacaaa tggatcaaga actatttatt
     8341 atcgaagtat tatcagctaa acaaaaaatt atcgagaaag cgaaaaaaag agcgaaaaga
     8401 ataatgttca agcttttaag cttagtattt ttctctggtt tgcttttttt cggagctgta
     8461 tcgttcctat atcgccatat gagtgcagta gtccgttcag ctgaagggat gcaaaaatta
     8521 aataatttgt atcacttttc gtggggagtt ggcggaataa caattgttgt gcttattgtt
     8581 tttcttctac ttatttggct ccgttaccat caattgtcgc gtcatttcga tcaatggctt
     8641 agtcaaagag cacaacagtt tttacataat aaaccaactt ctcaagatga tgactatctt
     8701 tattttgaga agacttcgac gagtcaagct tttcggatac aaaaaagtga ttgccagtta
     8761 ttgctgagat cacttgatga tttacctatc tacttaggcg aaagcaaatc aaaattcgga
     8821 atgaacacat tccatatttt tttactagaa aacgaaccca gatgggaaaa aagccagtct
     8881 tcgcctggtg gatacctgac aactaagatg agtacattag tcactggttt tttcctcatt
     8941 cttttagctg gaacaatcta ttatgggaga tacactcagc caaaaatgtc ttctcatata
     9001 aatgctgctt ctcacatgac aatgaactca actaacgata cgcatataca aaagacaaat
     9061 gaagggttgt tagctgatcc taagcaaaag gtaaatttga caacacaaaa gataaatgat
     9121 ctcaaacttg atacagagac gcactctctc tatatgacaa cagatagtgg tgctaattgg
     9181 gtatttgtgc cgattgattc gcaatggttg cgttttggcg aatacaccct gacaacgggg
     9241 ctgattcctg aaggttattg gatggacaac acatatgata tttccgccga tttctcatgg
     9301 tttatctatt cttctgggga tgatgtacgt ctgctgactt caagagataa tggtaaaaca
     9361 tggcaaacgt ctattatcac ccaatcagtt cagccgatac gttatcgaaa agcggtattt
     9421 tatggcaacg atggtctatt tatctgtaca tcagccagtg ggatgtctgc agaaactttg
     9481 tacatttaca ctactatgga tggcggaatc acttggataa aaagtggaag tacaatgatc
     9541 aatcagccag tacaaaatgt cagcttcgtt acaacttctt taggttttgt ttcgacacgt
     9601 gaaagtttgt tttatacaaa caacggagga cgctcattca aagaatcagt tctgtcgatt
     9661 ccggaaaact atcagacagg ggggatagat ctttttcaat caccaaatga agttactcaa
     9721 ataagcgcga atacgttaga gacaaaattt tatttgttga aaaaaggacc aatcgatgca
     9781 gggaaaatgt ttgcctgcct cttccgttca actgataacg gggaaacatg gcaattcgtt
     9841 cagcaactat cgcaagtaga acaaggagat taaagaagag agattgtgag cctgacgttc
     9901 agcaccgagt cacaagaaac aaattcaaaa aacagcttct catatttgtt gaatagtgta
     9961 actcgtgatt cggagttgac aagagcaaca agaagcgatt ttgaagtata gcttctcgta
    10021 ttttacaaga tcgtgtctta ttgccgatag gttgtctctt gaacaccgtt tattcgtaaa
    10081 aagggattgt gacaagtctt ttgtcactac ttaccgaacg aaactgaagg agctatccaa
    10141 aaagtatcat tagtattatt ctttgtgttt ctagtggcac cactagtaca tttaaaaaag
    10201 ctatcgatca tggactaatc atacatgatc gatagctttt tagataatgt ttgttacgct
    10261 atctgaaatt gactattata aagactggca taaagcgttg gtgattggag tagttcagaa
    10321 tgtgttcctt tttcgacgat atcgccattc ttcattacaa ggatcatgtc tgcattttca
    10381 atcgttgaca aacggtgtgc aatcacaaaa cttgtcctat cttttgtcac agcttccatt
    10441 gcttgctgaa tcaatgcttc tgttcgtgta tctacgctag aagttgcttc atctaaaata
    10501 acgacaggag gattcgctag aatgactcga gcaattgtca ataattgttg ctgtccttga
    10561 gataacgtgc cgttttcgct agaaatgatc gtatcatatc cgtctggcag tgttcgaata
    10621 aagtgatcac attgtgcgat ttcagccgct tcgataattt gttctcttgt ggcatccatt
    10681 ttcccgtagg cgatattatc agctaccgtt ccttcaaaca gccaggtatt ttgtaaaacc
    10741 atcccaaaag aacgacgtag ttctccacgt gaaagcttgg taatatcaag accgtcgaat
    10801 tcgatctttc ctttattcag ttcataaaaa cgcatcagca gattgactaa agtcgttttt
    10861 cctgcaccag ttggaccaac gatcgctacc gtttgttttg gctttactga aaaatcgact
    10921 tgattcatca atattttatc tgatgtgtaa ccaaactgta catttttgaa agctattttt
    10981 cctttcggtt gttctaaaag tcttgcatct ggaaggtctg gaatttcatc tgtttcatct
    11041 aatatctcaa atatcctatc gattgctgcc attgcggatt ggatcgagtt gatcacataa
    11101 gaagcggtag aaattggttc agaaatctga ttgatgtact gaagataagc ttgtaagaag
    11161 ccgatcgtca caccacctga tagaacaagc atagcaccga ataccgcact tatgatgaag
    11221 gcgagttgat taatgaaacg aatggccgga tagatagcac agttcataaa ttgagcacgt
    11281 ttaaaagcgt tgtattgctt tttatttact tcgtgtatag cagcttctgc atgagcttgc
    11341 tgattgaatg ttttgaccac tagattacca gctaagattt cttccattcg attatttaaa
    11401 acacccagtt catactgatt ctgttcagaa atctgtttgt ttttgttcgc aatcttagtt
    11461 gtaacaattg aactgccaac gatcagcaat aagacgagca aagtaagctt gctttcgata
    11521 taaaacagca tcagaccagc agcagctatt gttacgacag aagtaaaaaa ttgattgata
    11581 cctgtcaata acacttgaga aagttgattg agaccggtag ttgtccgact aatgatgtca
    11641 cctacttgat gattgtcaaa aaacgacatc ggtaatgttt tgaatttttt tgtgatttct
    11701 tttcgcaccc tcaatgtgat cttttcgctt aacgctgcca ttgtccgttc ctgaataaat
    11761 gaagtgatgc tgcttaagac tgaacagccg atcagcaata agacagggaa caacaatgct
    11821 tgtttgatat cactgaaaga aagctgtcca atacctgttt ttcttatttc tgataagaga
    11881 tcatcgatcc cgattcccat gatcaagggc atggctacga ctaataggtt cccgaataaa
    11941 ctgcatagaa acaatccgta aaaatattta tgttcaggtt tgatcatttt ccaaaaacgt
    12001 atcaaagcac gcgtgttcaa cgtttttttc ttttccatcg tatcccttct ttctatattc
    12061 cttgtgattt tgcaaactcc tgataagttt ggcaattaga cagtagttct tcatgagtac
    12121 cttgtccaac gatttttcct tcatctaaaa caatgatgtt atctgcttgt tggattgtac
    12181 tgaggcgttg agcaacaatc acgagagttt tctccgacat gtgtttatga agggctgctc
    12241 ttaatgctgc atctgtttta tagtctaaag cggagaaact atcatcgaat acatagatct
    12301 ctgctggttt gatcaacgct cgagcaatac atatccgctg tttctggccc cctgaataat
    12361 tagaaccgcc ttgagcaaca aagctgtcca atccgtcagg aagggtgtcc actagttctt
    12421 ctaattgagc gatcttgatc gctttagaca actcttctgt tgaggcatat tcatttccca
    12481 tctgcaagtt gcttaaaatt gtcccgctga ataagaaagc cttctgaggg acataactga
    12541 tgtgggagcg gatctgttct tgagtcattt gagtaagagg aataccacca agggttactt
    12601 ttcctatcgt agggtcacta agtctcaaga gaagtttcgc caaactactt tttccagatc
    12661 cggtcccgcc aacgatcgct gttgtttttc ctttaggtat tgtgaaatca atatcttcta
    12721 gaactggatc agagatttca gtatatttaa atgtgacatg ttcaaaagca gctgcatttg
    12781 tctgatcaaa ggttggttca gctactgttt tgatcgtctc ttcctcaatt tcgatctctg
    12841 cttgcagtat ttcttctatc cgtcgtaatg aggcaaacga tttagggata atgaccaata
    12901 ccatagctgc gatcatcaag taagcaagtg tcagcataga gtattcgact acagcagtca
    12961 cgacaccgat ttgtaatgct ccttctgcta caagatttcc gccaaaccac aaaatagaag
    13021 cataaacgat tcccatgagc aaaaaagcaa ttggagtgac ccaagcaaat attcggttca
    13081 ctttgatcat ttgattagca tagtcagcaa agctcttatt cgttcgcttt tcttcatatg
    13141 attgattatc aaaggcacgg atcatattaa tcccagtaaa gaattctctc aatgtgatgg
    13201 tgatccgatc catttttggc tggatactca aagataaagg tgcccctcgt ttcatcagtc
    13261 gataaacaat ccatacataa aaaacaacag agatcaatgt aacccatgtt agtaatagag
    13321 aataacgaaa catcatgaga atggtgaaaa cgcaaataac cggtgctggt aagatcattt
    13381 ggcaaaataa aacaatcatt tgctggacat tatcgacatc attcgtcatt cttgtcaaaa
    13441 gagatccaga actaaaacga ctgatatcct tcattgacag ctgttggaat ttacggaaat
    13501 atttctcccg cgtttggaat ccaaacctag cggaagccaa tgctgaaaag taactagagg
    13561 caactgcagc gattgttcct agaaacgcta caagcagcat ttcaactcca atttttttta
    13621 taactgaggc ttcgccactt gctatacctg tgtcgatcag ctttgctact aattttggta
    13681 cacctaatgt accggcgatt tctaaacaga tcatcacaaa ggtaaggaga gcgagtcctt
    13741 tgtttttctg taagaatgtt tttaagatag acatattatt cctccttttt tcctgatact
    13801 tttcgattct ataccttcaa gtaacttgaa ggtcaataga aaaaagaata aactgaaaaa
    13861 gaggtgagaa aaaatgaaaa aaatgtatac gattggccaa gtatcggaac tatttgattt
    13921 gccaaaatca acgatccgct attgggatga acaaggatta atccgttcaa gtagacaaga
    13981 agagaatgat tatcgattgt ttgatatcga tgatattttt atgctttatg atatcgtttt
    14041 ttatagaaaa ttagatatac cgatcaaaca gatgaagaat ttatatggaa aaacattgac
    14101 ggagttatat gagacacttg atgaaacaga aagaagaatc cataaagaac tggtggtgat
    14161 gaaacaaaaa caaaaagaga taagggagca aaaaaaacag ttgaaattga tgatcgatac
    14221 aaacgaggaa gaatttcctg tagaagagat cccttttgac tgtatgatct caacggagtt
    14281 tgaagatatt gtggaaatca aaaaatttct acctaactat tcaagctttg ggatgatgag
    14341 tatgccagct tccagttcac agacgatgta tggatttttt atcgacccta gtgaagtaca
    14401 tttatttact caagacgtga tttgggagaa aaaagatacg gctgtttatc gtcgattctt
    14461 attgaaatcc gagatgaacc atgcggaacg taataatatt ttagaaatca gagaaagaat
    14521 gtatgaaaaa ggatggaaaa caggagaagt gatcggccag tatctattga cgaatacgga
    14581 tgaaaacaat atcagaactg aatattatca tgcatggata gaaatgaaaa aataggaaat
    14641 atgtgtttaa gataactcga aaatgatggt tgttttagtt ataaagaaaa attttctggt
    14701 tcgattacgg gtttaatgtg ggggattatt agtcgtggat agatagaatt ttagtttttt
    14761 agaggaaata tgtctttaaa aatggaatgg ttgcaattct tagcgatata tacgatgtaa
    14821 ttgtcctctt aaaaaagtat ggaaaaataa ttgtattggg tgttatgtat aattcatggt
    14881 gaactgagac taatgagaag aaaaattcat attgttatga gtgagattgt atcagatgag
    14941 aatgataaat taaataggtg cataaaagca ttacacttaa aatacattgt tgcttacatt
    15001 attacaatat gttaataagg caaaaataaa tagatggatt gaataaacat attttttaat
    15061 caaatagtcc ctactgtgac tttcctcagc tatcggtgat actaccgata gcattttttt
    15121 gcttataaaa taattataag tatttagaga tttttcttct aacattcttc aaacaagaaa
    15181 aatcacaaag gttattgtta tttttatata ttctttttaa gaatgattgt ttttttatag
    15241 aaattttaaa aaataaaaca cgttattgat ggatagttag tttgttattt ggtaatatta
    15301 tagaagttga ctggtagtga tttatttttc gtcgttttat aaaaatgtta ttttttggaa
    15361 aaattcacaa atatgtaacg aatgtttttc tggaatttgc tgtgttaaaa agcagtatga
    15421 ctaatataaa tactattgtc atttatgcaa ttttttatgg atagtaattt tagaggggaa
    15481 tcaatgaagg aagaatagaa gggaaaaata gttgcaaata cgccagttag caaaaatagc
    15541 aatacatctt caagctttaa aattcgagag aatcatcttg gtagtggaat caactacaac
    15601 aaaaggatta aaccaattaa tagatagaat taccaatgga aaaatagaaa aggtagttat
    15661 tctatacaaa agttgtttaa ttcgcttcgg ttatgaactt agtgaaaccc tttgcaatat
    15721 atggtacaga atatagaaat tattgacatt acagaaaatg atgaagattt aatacagatt
    15781 gtcactattt gtagatacct tttacaagga aaccgagcca ataaagctaa gaagatgatt
    15841 aaggagttga tggaagacga tacaagttaa gaaagtgaaa ctacgcccaa ataaagagca
    15901 aaaagagtaa ttggacgtgg aaaatgcaag aaatgaacca tcgattcggt ggacagttta
    15961 ttagtaatga cggtttgaga acatccatga cgagaatgaa ggaacatcct aaatgcgttt
    16021 agttgaacca agtgccaaat aatatggcca aataagcgat cagttatcga tgaatcccaa
    16081 gtattctaaa ctatatattc attttgatgc taagcattga tatatttttt tgggattgat
    16141 gaaggttaac caatacaggg actcactgat gaaattctag agtctgacct tggtatcaac
    16201 gatttagtga ttgtttctaa tggaaaagta taaaagtata taagtacatt aacaaaataa
    16261 ctattagggt tacgctccag cttgtgcaag gcactggcag gaggaatcgt gaaatcataa
    16321 ttatggttaa gccacacgga caactaaaag tcatacaaag ctatcgaatc aatcaacaga
    16381 agacaatcgg caatcattag ctttgtttac agcttacatt gcaacgcttg agttgaaccg
    16441 aaaatacgag ggaggtaacg cagccatttt gagttgggga atctttttag aaaattatcg
    16501 ggtaccttgt tgtttagtca aaattataag ggggagtaat agtgaggaac aaatttcata
    16561 aactgttaag tatgtttcta gttatgctta ttgtgatcaa tcaatttggg aacataacgg
    16621 ttctagctga gtctttagca gaacagccag agcgttcgct gacgatcgaa gttgatcaag
    16681 aggaggctgc tttgggtgag cagttccagt tgcgagtcag aggaacacca gacctgctaa
    16741 atgcattggt cattgataag agtgaggatt tgattgaagg ggaagtgaag caactcaatg
    16801 attacgaacg tctgattact tattcttccc agcattcagg taaatacgaa ttgaccgcaa
    16861 gaagcggtga tattcaatca aatacggtct caatagaaat cagtgtacca gaagaaagca
    16921 caagtgagtc ttttaatgta ccagaagaaa gcacaagtga atcttttaat ataccagaag
    16981 aaagcttaag tgaatctttt aatgaagcca gatcatctga gccatctata acgcaggaat
    17041 ctcagattga aagacaagag gagctcttga attatgacat cggcatctct gctgagcgcc
    17101 ccactttgcc gagcaggcat acacaaacgt taaagggata cattcaagga ggcgctggcg
    17161 atattcttga agaatatggg gagatggtcg tacgagttcc tagagcatcg attggcttaa
    17221 ttgagttcat tagagtggag gtcgctgatg attcaccttt aaagccactg gcattttacc
    17281 aagaaggcac agacgttgtt tttccattta cctttgatta tgaaggatta aatgaagccg
    17341 ctcagtcgta tacattcagt atcacgtttg aagttgcttt ctttcaatca ggcggtactg
    17401 agccaaataa tgactttcaa gtgagtctta cttatgctga gcgagcagca aacacggaat
    17461 tttcaattac gcctattcct tcatcaagac ccattttttc gaaattatac tatggctcta
    17521 gagaaatcga cagccaagga aatcttggga atctgctgat tgacgggcaa gacgtgacca
    17581 acagtaataa atttattttg caggtaaatt atgaaggatc acgaaaagac ggtgtagtgg
    17641 ttaccgatgt actaccgcaa ggaacaactt tggcaccgaa tccgactaca tggagttcca
    17701 ctggaaatag agaacctatt tcaggtgggg ttcggatcgc agaagtaacg aatccaggtg
    17761 aagcaaacgt tagaactcat tatgtttccg aggaatttgt ggacaaagta tcttttaata
    17821 aggaaacgaa tacacttgtt gtggactttg gtaatctagc agagggtcaa tcgtatttta
    17881 ttgaatatgc tcttgaagtg accgatgtcg acttgttcaa tgaaggaaga caagaggatg
    17941 ggttttctcg gaatcgagcg agtatgacct acaataatca ggtaatgagc cgagactacc
    18001 gcttgagaat ccctgaaagt aatcttccgg caatctcttt aagaaaaagt gtggacaaaa
    18061 atgttatcaa tctcaatgaa aatgaactaa cgtatactct gcatgtctcg atgttttctg
    18121 gaacaagaaa tacgcttgtt attgaagatg ttttggaaga aggcatggaa tttgtgtcat
    18181 ttctatcaga tattccctct ttcattgatg ccgaagtcaa taatggaata gttcgtttca
    18241 gtgcgaataa tacgattggt ggtccgacaa gttttaccct ctcttttaag gtcaatgtag
    18301 aggcgtatgc ggtcggcgat caaattttaa actttgcaac gcttgtgaat agtgacaatc
    18361 gattgaatac gagtactgta agaacaagga aaatcgatgg gcgtattaaa gtaattaaaa
    18421 ctgatgagaa tggccaaagg ctggcaggag caaaatttga aattttatca agcgattcac
    18481 aagtgataca agaaggtata acggcagaaa acggagaatt tttaagtgaa ccgcttcctg
    18541 taggtagcta tcaagtccga gaaacagaag caccggaagg gtatctttta gatgcgacgg
    18601 tacatgatgt ttttataaat gaatactcca tagaagaagt gacgttaacc attgaaaaca
    18661 tccttgatac gggtggtgtt gaactaatta aacatgctga aggcagtggt gaagtccttc
    18721 aaggagctgt ttttgagtta cagaaccgag aaggagaaac gctacaaacc ggtttaacta
    18781 caggagaaga tggaaaactg gcaattgacg gtttagcacc aggagcatat cagctggtag
    18841 aaac
//
LOCUS       DACKAC010000053        18527 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.053, whole genome shotgun sequence.
ACCESSION   DACKAC010000053 DACKAC010000000
VERSION     DACKAC010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.053"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(99..1619)
                     /locus_tag="I0B46_001954"
     CDS             complement(99..1619)
                     /locus_tag="I0B46_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAR2084234.1"
                     /translation="MTKMESELLVQIKQLTQLLEIQQQENTLLREQIAEMNRRLFGRK
                     KETPPVDGQIDLLDDSTFNEPEHTGQESQEPITVSSFKRRKRKGLKALSLEGLPEVPI
                     HYELKGADCLCEDCGHTLHDIGATRLREEPLYVPAHIEKQVIYQHAYHCRECEALGET
                     KIKKAPVPKPLINNSLGSSSIVTQSIWEKFVKKVPAYRQEKEWHALGFPLDRKKITNW
                     HIKVSEMVLEPIYNCLHQELSNQEVLHADETSYRVLNTHKEKTFYWLYASGKSEEKQV
                     VLYEHANSRSVDEPKSFLESFSGFLHTDGYAAYGKLPVNNVYCWAHVRRKFFEALGKQ
                     PSPKSHAKIGLDFCDKMFRIERTFNKLSAEERFVARKETLQPVMTEFFHWCESFAYLP
                     KSQLGKAVQYAVNHKDGLQIVLLDGRLELSNNRAERAIKELVIGRKNWLFSKSLKGAR
                     SNGIILSIIQTAVANGLNIRKYLNHLFTEIPNLSSMTPEALRAYLPWNQQIQEICK"
     gene            complement(1862..2047)
                     /locus_tag="I0B46_001955"
     CDS             complement(1862..2047)
                     /locus_tag="I0B46_001955"
                     /inference="COORDINATES: protein motif:HMM:NF017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HAR2084235.1"
                     /translation="MPMLIDATALKHIFIVCGKTDLRKGIDGLAAFVIEEYDLDVYDQ
                     ALFLFCGTKKDRFKALY"
     gene            complement(2031..2213)
                     /locus_tag="I0B46_001956"
     CDS             complement(2031..2213)
                     /locus_tag="I0B46_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084236.1"
                     /translation="MNKKDIVPVQFSSAAQKSQIDTKPKLACRIKSEDKEIFIYNGMD
                     KYLLYTLLKELNANAH"
     gene            2454..2672
                     /locus_tag="I0B46_001957"
                     /pseudo
     CDS             2454..2672
                     /locus_tag="I0B46_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            3050..5092
                     /locus_tag="I0B46_001958"
     CDS             3050..5092
                     /locus_tag="I0B46_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAR2084237.1"
                     /translation="MNKRIREILKLIIKNPEMKLSTLTSELDLTRRQINYAINQFNEE
                     LKTKNIPLIQRSHSGNITVPIEVIQMISQPDYDIGDDQELVVLTDSERQALIVMTLIA
                     NIDYISLDHLVDAVDVSKTTVMEDIKRTEPFIRNYSLTIRYNRMNGYQLVGSEHRILQ
                     LLSDLVKRFPIFRREMIRRKLAQDVSEEEIVHLIHNMEQMLHLSYSDESVDYLQTSAR
                     FLVSRGIKMSDKHAFFPDDVTDTPEFRMLMILVHETGWSLSKSYMEWFTLLFLTSNIF
                     ESKTTQNYDSDQELRKLISQMVENFQNQTLILIDDREDFERRILSHLRPACFRIRYNL
                     SLGVYSIDSLIKDSNHAILIDLMKELIIPIENWLNKAFPNDELDLLSYYFGYQLSNHN
                     KLNKQRQRAVVVCTNGVMVSKLVRANMEKLFPEIHFLASLSVRDFYKFEADYDLVFTT
                     TPLKSSMLQFMIEPIMTYQQQISLRYRVLSDLGITKVDRAMDELLTIIRKYANITNPV
                     SLREELQYFLIKEDGSTPLDNFNVLPSLTYYLRPSYVQIVEEEMSWERAVEVACRPLL
                     EHQIIDSRFIADCLDQIRQPGYAGYLGMKTCIPHTTVDKGVISDGVSILVCKKPIRFP
                     NGEDVSLILPLSFFDLTKHLRAINQIADIAKDDSLIEQLLIADDEKTVYQLLRQRS"
     gene            5103..5723
                     /locus_tag="I0B46_001959"
     CDS             5103..5723
                     /locus_tag="I0B46_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAR2084238.1"
                     /translation="MRLADYPKLTVIMRGYTFDQADAILQAMQEFDHQFAVEMTMNTK
                     GALENIHELNQRYGDTTYIGAGTVRTLEQAQACYEAGAKFLLGPHMFTKEMLSYANKK
                     GLLAVPAAMTPSEVDRMFKEGADIVKVFPAAVVTPRFFKDIQAPLGKLPLMGVGGISK
                     ENAKEFFEQGASYLGLGSGMFNKQDIEELNVKNLARSMKELIEAIK"
     gene            5735..6193
                     /locus_tag="I0B46_001960"
     CDS             5735..6193
                     /locus_tag="I0B46_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAR2084239.1"
                     /translation="MELYLNEELVFRNVQAKTDSEVLAFLASEMYKKNYVKEEYIQAI
                     QEREKEYPTGLPSTPPGIAIPHANYEMVNKTTLAIATLKDPVLFHNMEDNNEQLPIQI
                     VIMMAIGEPHGQVEMLQKIVGIIQDEPLRQEMIRAGNDTELLELLKKAVF"
     gene            6212..6490
                     /locus_tag="I0B46_001961"
     CDS             6212..6490
                     /locus_tag="I0B46_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAR2084240.1"
                     /translation="MKRILVACGNGIATSTVVATKVREYLQQHGIDVSTTQTKLMEVP
                     GKVQDYDLLVTTGQFDGQTGGVPVIKGMPILTGIGADQTMEEILNLLK"
     gene            6515..7882
                     /locus_tag="I0B46_001962"
     CDS             6515..7882
                     /locus_tag="I0B46_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002386123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAR2084241.1"
                     /translation="MKIIMNVFNFFIDAGPTVMLPVIITIIGLIFGLKISRAFKSGLT
                     LGIGFAGIKLILDFMTTNVGPAAKAMVDRTGVKLDALDVGWGSIAAVTWASPIIPILI
                     FAILLVNIVLLILKRTHTLDVDIWNYHHMAIVGVIVYFVTKNVFLGVGASVVMAIATF
                     KISDWSQPMVESFFGIPGVSLPTVSALSSLVIAWPLNWLLDRIPLFRKSKFTIKDAQK
                     YLGFFGDSMIMGLVIGMVIGVLAGYDIKAVLQLGVSMSAVLVLIPKMTSLFMEGLMPI
                     SDAAQKWSQKKFKGTRLFIGLDAAVVVGNPDVITTALIIIPLTIALALVLPGNRVLPF
                     ADLAVVPFRVAMVVALTRGNLLKNIIIGLVVTASLLWCGSATAPMLTAIAKSVGINLG
                     TSSMLISSFAATSMIQSYLVFIAFAYKPIIGIPVLLLAFAGAWYYFDGIKKVNHEMDE
                     PLKAQ"
     gene            7897..9042
                     /gene="dgoD"
                     /locus_tag="I0B46_001963"
     CDS             7897..9042
                     /gene="dgoD"
                     /locus_tag="I0B46_001963"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HAR2084242.1"
                     /translation="MKISDVTVYKVKPRWIFVKISTDEGIEGWGEMISGTKTETVVAG
                     AYEIGNRLIGRNPFEIERLFQEMHRSFFRGGPINGTIVSGLEMALWDIKGKAFNVPVY
                     ELLGGAARDKIKVYSWIGGDRPSEVAEQAQDRFDRGFTAVKMNATEELHYIDSYQKVD
                     EVVERVASIRERFGDAMDIGVDFHGRVHKPMAKVLAKALEPFHPMFLEEVVLPENEEH
                     FKEVADSVAVPLATGERLYTRWQFKNLFKQGTVDIIQPDVALCGGILETRKIAAMAEA
                     YDMAVAPHAPYGPVALAATLQVDSCTPNVFIQEQSLGIHYNKGFDLLDFVKNKEVFQY
                     KDGYVDLPSKPGLGLEMDEDKIKEIAQEGLIWTNPQWKNYDGTIAEW"
     gene            9069..9560
                     /locus_tag="I0B46_001964"
     CDS             9069..9560
                     /locus_tag="I0B46_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084243.1"
                     /translation="MAEALMITVFLGLLIILQIIDQKKIIVFAQSSPVRKSLSILLTV
                     AILLIFWPEHLTDQIKLVAFASVILINGFLKEGLAKDSVVRFGVLNGNFNRYPQVQVE
                     KLGDDKSFISFYKSKNNSFSMIFDKDQTEMMDVFQELDIHSKITLDQSSEEKRAVVKK
                     DNE"
     gene            10003..11202
                     /locus_tag="I0B46_001965"
     CDS             10003..11202
                     /locus_tag="I0B46_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAR2084244.1"
                     /translation="MRDQTSGKMIRFYYTSLHFLYWMMFCSVYGFATLFLIYFKIDPA
                     KIGGILALVNIVSTVLQPFVSQVIIKKRKIPLITVLKTMIAGLGVILLAGVLIPNLPI
                     ITYILGGILVVSMQSFLNALAFEYSNTGYRINFGFSRAGGSFSYAVTSFLLGQLLAIY
                     SAAILPILVIVEAVIFFVILHLLPDVKNQEVFPMTKEQDRSVSLRKKYPFLILLFLGF
                     SFLFTFHTMINSFLAQIFENIHGGSKEVGIALMIAALCELPGMIFFEQLVKKKSSKFW
                     LSVASISFAVRGFMMLLASSILMMEMTHLLQAFSFALFIPASAYLFNQFLADDDRVFG
                     QTMIIIATTLGGVIGNVLGGSLLQHFGVWEMLIFGTGFAVLGALLTILGIRKIPKTGN
                     DSDSLVE"
     gene            complement(11697..12455)
                     /locus_tag="I0B46_001966"
     CDS             complement(11697..12455)
                     /locus_tag="I0B46_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAR2084245.1"
                     /translation="MDTKLSESEKYLWDFIQSHILEIPSYSIVKLSEYANVSTATVVR
                     TMKKKGYEGFTSFKHSLKEKENTNINFAALDKVDEGIKTAILKNEQEVIRTINMVESG
                     NIEDAIQRIKAARRIMIFARGFSELIAQEMMVKFQLTGKYCEMHTDPNIIKNISRKLS
                     KDDIVIFVSLNGETKELTTAAENCYKAEIGTILLTANRHSSLMEHIEIPFVGFKSEGS
                     FFPDYEVRSRLPLSILARILLDSYALRTSGFTQH"
     gene            12623..13558
                     /gene="pfkB"
                     /locus_tag="I0B46_001967"
     CDS             12623..13558
                     /gene="pfkB"
                     /locus_tag="I0B46_001967"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAR2084246.1"
                     /translation="MSVYTCTLNLAIDLFIETDEMKPSQVNRTNDDDIQANGKGVNVS
                     LIMKQLGIDSTALGFKAGFTGNYIEDYLHQKDISTDFISVPGMTRINVFTQVNKTNEE
                     FKLVNKGPEIPQEQIKTFLNQIQKLQADDYLCVSGSLPRGVSPEILIDISRICFENKV
                     HLIIDSSYPEIIDCLPYQPFLLKPNEEELQSWYNVELSDREGYIHYAERLIADGAKNV
                     LLSLGGEGGIFLNKDIVYSGNSPKGKVVNTACAGDTLLGTFLAGYLKGEQVDINLKKS
                     LAAGSSTAFRKGLTDFSDVAELEKQIEIKEGASKK"
     gene            13555..15000
                     /locus_tag="I0B46_001968"
     CDS             13555..15000
                     /locus_tag="I0B46_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAR2084247.1"
                     /translation="MTTYKIVAATGCPTGIAHTYMAQEALEQAAKRKGVSIKVETHGQ
                     VGIENELTPEEIKNADAVIIAADKDVQAERFAGKRVIDVPVSKGIKDADQLIEDALAG
                     KGSITAENKTVDAAGEKAEMGVAAGIGHSIYKNLMNGVSHMLPFVVAGGVIIAISFAI
                     WGVYSFDPNDPTYNPTAAMLKGVGDAAMGMMVPILAAYIAEGIAKRPGLVVGFVGGLL
                     ANSGGAGFLGGIFAGFLAGYFILLLQKVMKGLPKQLDGLKAIFLYPVIGVLFVGVVMS
                     LLANPMEAVNQGMMNFLAGFQNSNPLILGIIVGCMSAFDMGGPVNKAAYVTGTALLAQ
                     GNHTFMAGVSAACIAPPLITGFAALFFGKYFDTNDRNAGLVNFILGSTHITEGAIPFA
                     AKDPLRVLPTMMLGSSIAAVLTYMFGVQVPAPHGGFLVLPVVTGKVAWVGSILLGSIV
                     GGVILGLIQKRSVEKREAEAAAVEMKEATNN"
     gene            15028..15483
                     /locus_tag="I0B46_001969"
     CDS             15028..15483
                     /locus_tag="I0B46_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAR2084248.1"
                     /translation="MTLIKTNKVFMNKNFTTQDEVMNFLADQSIVLSISEDRDAVYEK
                     LMEREEESTTGMMDGFAIPHAKDASIKDADIVIVRLNGKVDWKSLDGEGTDFVIALFI
                     PDSEAGTTHLKFLSQVARLLMHKDVTEGLKQAQSAEEIAELLNQKLEEN"
     gene            15502..16476
                     /gene="lacD"
                     /locus_tag="I0B46_001970"
     CDS             15502..16476
                     /gene="lacD"
                     /locus_tag="I0B46_001970"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAR2084249.1"
                     /translation="MPLTEKKKTYIRQLSNESGIISALAIDQRGALKKMIGKFKNKSA
                     TDEQIINFKKIISSELTPYASAILLDPEYGLPAAEVRDKNAGLLLAYEKTGYDASTPG
                     RLPDILSIWSVKRLKEQGADACKFLLYYDVDESDDINDQKKAFMERIGSECVAEDLPF
                     FLELVSYDANDPDTSSKEYALKKPHKVNEMMKEFSKPRYGVDVLKVEVPVNMNYVDGY
                     TKNEVAYTKEEAAAYFKEQSETTNLPFIFLSAGVTAELFQETLKFAKEAGSTFNGVLC
                     GRATWANGVEPFVTKGEYAAKEWMQTQGRKNIEELNNVLKETATAIKL"
     gene            complement(16587..17148)
                     /locus_tag="I0B46_001971"
                     /pseudo
     CDS             complement(16587..17148)
                     /locus_tag="I0B46_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            17292..17906
                     /locus_tag="I0B46_001972"
     CDS             17292..17906
                     /locus_tag="I0B46_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAR2084250.1"
                     /translation="MELAFKQLEKKDYKKAIQFAIKGMHFDWYTKNKLLLNLYGRYFW
                     YLELNRATQIIALYADDELAGVLLADIKGKSKKHHSFSQKLYVKLFDFLQNAFVKDSK
                     GGYDDTNDELLSDYLKKHSPDGEIVFLAANPDLKIKGIGSKLLKEFERREQGKEVFLF
                     TDSGCTYQFYERRGFERKKEKDNVMKILNKEVDLRCMLYSKIIQ"
     gene            18147..>18527
                     /locus_tag="I0B46_001973"
     CDS             18147..>18527
                     /locus_tag="I0B46_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAR2084251.1"
                     /translation="MPKYKEIALALKERIAEGKYQEGSKLPEQESLAREFKTSRVTIR
                     KAIQLLIDEGLLYTRRGSGTFIRSNIRQDNENVTQVNNVFGTSSQENTNVTSKIIRFD
                     VRFPTDYERRLSATYFRSPSCKSWV"
BASE COUNT         5764 a         3124 c         3810 g         5829 t
ORIGIN      
        1 ataattcagt agttttgagc gtaagcgtgt catagaatgg tacctaaaca caaatagccc
       61 gatttgatac actcaaatca ggttatttgt ggcttatatt atttacagat ttcttgtatc
      121 tgctgattcc atggcaaata agcgcgtagc gcttcaggtg tcatcgaaga cagattggga
      181 atctctgtaa aaagatgatt taaatatttg cggatgttta acccattcgc aacagctgtc
      241 tggataatac tcaatataat tccatttgat ctagcgcctt ttaagctttt agaaaatagc
      301 cagttttttc gaccgatcac taactctttg atggcacgct ctgctcggtt atttgaaagt
      361 tccaagcgtc catcaagcaa cacaatctga agtccgtctt tatgattgac tgcgtattga
      421 accgcctttc caagctgcga ttttggcaaa taagcaaaac tctcacacca atgaaaaaat
      481 tcagtcatca caggctgcaa cgtttccttc ctcgctacaa acctttcttc tgcgctaagc
      541 ttattaaagg tccgttcgat tcgaaacatc ttgtcacaga agtctaatcc tattttggca
      601 tgactttttg gggacggttg tttacccaat gcttcaaaaa actttctgcg aacatgcgcc
      661 caacaataaa cattgtttac cggcagcttt ccataggccg cgtaaccatc agtatgcaag
      721 aaaccgctaa atgattctaa aaaacttttt ggctcatcaa cggaacgcga atttgcgtgc
      781 tcgtagagca caacttgttt ttcttcgctt ttacctgaag catataacca gtaaaaggtc
      841 ttttctttat gagtattcaa tacccgatag cttgtctcat ccgcgtgtaa gacctcctga
      901 ttgcttaatt cttgatgaag gcaattgtag attggctcta aaaccatttc acttaccttg
      961 atatgccaat tagtgatttt tttacgatca agagggaaac caagtgcatg ccattctttt
     1021 tcttgtcgat atgccggcac ctttttaaca aatttttccc aaatactctg tgtcacaatt
     1081 gaagaggaac ctagactatt attgatcaac ggtttaggaa caggcgcttt tttgattttt
     1141 gtttctccta atgcttcaca ttctctgcaa tgataggcat gttggtagat gacttgtttt
     1201 tctatatggg caggaacata caatggctct tctctaagcc ttgtcgcacc gatatcatgt
     1261 aaagtatgac cacagtcttc acacaaacaa tcggcacctt ttaattcata atgaatagga
     1321 acttcaggca gaccttctaa tgaaagtgct ttgagtccct tgcgttttct gcgtttaaaa
     1381 gaagataccg taatgggttc ctggctttct tgtccagtgt gctctggttc attaaaagtt
     1441 gagtcgtcta ataaatcgat ttgcccatct acgggtggtg tttctttttt tcgtccaaac
     1501 aagcgtcgat tcatttcagc aatttgttct ctaagaagag tattttcctg ttgctggatt
     1561 tcaagcaact gtgttaattg cttgatttgt acaagaagtt cagattccat ttttgtcatt
     1621 tttacaccac cattctagcg attttcttac atctattata ccattttatg acaacgaaaa
     1681 acagacaaaa tccttgattt aacaaggttt tcgtctaata tatcgcacct tttttcgttt
     1741 cttttagcgt acatttttct tcgattgaaa ggccagtcag taagacttct tttctggaac
     1801 gcggccattg aaggtaccca ctttcaaatc ttttgtacat taatacaaat ccattttgat
     1861 cctagtaaag agccttgaag cgatctttct tcgtaccgca aaataagaaa agcgcttgat
     1921 cgtaaacatc aaggtcatat tcttctataa cgaaagctgc tagtccatca attccttttc
     1981 gaagatctgt ttttccacaa acgataaaaa tatgtttcaa tgcagttgca tcaatgagca
     2041 ttggcattca actcttttaa caaggtatac aaaagatatt tatccattcc attataaata
     2101 aatatttcct tatcttccga tttgattctg caagctagtt ttggcttggt gtcaatttgc
     2161 gatttttgag ccgcacttga aaattgaact ggtacaatat cttttttatt cattcgtttt
     2221 tctccttcta tatttgatag tttaattata gaagaaagat aatcggcgta cgagatacta
     2281 tcctatgaca cgcttacatt taatttaata ttttgcctca aattagccac actttttata
     2341 gaactaagag gaaaaatgaa gttttcttcc tattaatata gttgtttgaa atcttaaata
     2401 tagcgtttta cgatgtaatg ggaagtatgg tgaataaatc aactatgttc gtacgaagca
     2461 tttactcaac taatttaatc gaatctttca ataaacaaat caagaaatac agccaccgca
     2521 aggaacagtt ccaaaatgaa gagtcgatgg aacgtttctt agtctcgtct tttgatactt
     2581 acaaccaaaa attcctaggt cgcagtcata aaggtttcca acaggccgaa ggcgagctcg
     2641 aacaaatgct gagtcaaccg atggagaatt agaaacaatc tacagtaggg aagaaccatt
     2701 tacacaaaat tattgacgct cccaaaagca ttgtttttag cagtttaata ttattctttt
     2761 tcaattagac tgacttgatt tagttacgga atagatactt taatgtgttg aagaagcaac
     2821 gtcccgtgtt tatttactaa agcatccaat attctaccga atatttaata ttcacattag
     2881 ccctaaaata attgtcatac catgttacat ttgatataac agcgttcatt ttaaaaatag
     2941 ctgttagtgt gacaaaacat taaaccaagt gtgacaaaaa acgtgatgaa agctttccgg
     3001 aaacgtatgt gaacaagtat ataatgattt tgtcaggagg atagtcaaaa tgaataaacg
     3061 aattcgtgaa atactcaaac tgattattaa aaaccctgaa atgaaacttt ccactttaac
     3121 aagtgaactt gatttgacac gtcgtcaaat caattacgcg atcaatcaat ttaatgaaga
     3181 attgaaaaca aagaacattc cgttaattca gagaagtcat tctggaaata ttacggtacc
     3241 aatcgaggtt atccaaatga tatcacagcc tgattacgac ataggtgatg atcaggaact
     3301 tgtcgttctc actgatagtg aaagacaagc attgatagtg atgactctga tcgctaatat
     3361 cgattacatc agtctggatc atttggtaga tgcggttgat gttagtaaga cgactgttat
     3421 ggaagatatc aaacgaacgg aaccgttcat cagaaattat agtctgacaa ttcgatataa
     3481 tcggatgaat ggttaccaat tggttggttc ggaacaccgt atcttacagc tgttatcgga
     3541 tctagtaaaa cggtttccga tttttcgccg agaaatgatt cgcagaaaat tggcacaaga
     3601 tgtttctgaa gaagaaatcg tccatttgat ccataacatg gaacaaatgt tgcatcttag
     3661 ttattctgat gagtcggtag actatttgca gacatcagcg agatttttag taagccgtgg
     3721 gatcaagatg tcagataagc acgcgttttt tcccgatgat gtgacggata ctcctgaatt
     3781 ccgaatgctg atgatcttgg tacatgaaac aggttggagt ctatccaaaa gttatatgga
     3841 gtggttcacg ttattatttt taacttccaa tatttttgag agcaaaacta ctcaaaatta
     3901 tgattcggat caagaattgc gaaaattgat cagccaaatg gttgaaaatt tccaaaatca
     3961 aacgttgatt ttgatcgatg accgagaaga ctttgaacgt aggatactga gtcatctgcg
     4021 tccagcctgt tttcgaatac gctataactt gagtttagga gtttattcaa tagatagtct
     4081 gatcaaggat agtaatcatg cgattttgat tgatttgatg aaagaattga ttattcctat
     4141 tgagaattgg ctaaataagg cctttccaaa tgatgaatta gatcttttat cctattactt
     4201 tggctatcaa ttaagtaatc ataataaatt gaataagcaa cgacaaaggg ctgtagttgt
     4261 ttgtactaat ggtgtcatgg tctcaaagtt agtacgagcc aatatggaaa aactttttcc
     4321 agagatccat tttttagctt ccctatctgt gcgtgatttc tacaaatttg aagctgatta
     4381 tgatctggtc tttactacga cgccactaaa aagttctatg ctgcagttta tgatcgagcc
     4441 aatcatgaca tatcagcaac aaatcagttt acgatatcga gttttgagtg atttaggaat
     4501 tactaaggtt gatcgagcaa tggatgaatt gttaacaatc attaggaaat atgcaaatat
     4561 caccaatcca gtttcgttgc gagaagaact gcagtacttt ttgatcaagg aagatggaag
     4621 cacgccgctg gacaacttca atgtattgcc ttcattgact tattacttgc ggccgagtta
     4681 tgtacaaatc gttgaagaag agatgtcttg ggaacgagca gtggaggtgg cttgccgacc
     4741 gttattggaa catcaaatca ttgactcgcg ttttatcgct gattgcttgg atcaaatcag
     4801 acaaccggga tatgcaggtt acttgggaat gaagacttgc attccccata caacggtcga
     4861 taaaggtgta atcagtgacg gtgtcagcat tttagtttgt aaaaagccga tacgatttcc
     4921 taatggtgaa gatgtcagct tgattttgcc gctttcattt tttgatttaa cgaagcatct
     4981 gcgagccatc aatcaaatcg ctgatatcgc caaagatgat tcattgattg agcagttatt
     5041 gattgctgat gacgaaaaga cagtttatca actattaaga caaagatctt aaggaggaaa
     5101 acatgagact agcagattat cctaaattga cagtgatcat gcgtggttat acttttgatc
     5161 aggcagatgc tattttacaa gcaatgcagg aatttgacca tcaatttgcc gtagagatga
     5221 caatgaacac aaaaggagct cttgaaaata tacatgaact aaatcaacgt tatggtgaca
     5281 cgacttatat cggcgctggg acggtaagga cattagaaca agcccaagcc tgctatgagg
     5341 ctggtgcaaa atttttatta ggaccacata tgttcacaaa agaaatgttg tcttacgcca
     5401 ataagaaagg attattggcg gtacctgccg caatgactcc ttcagaagtc gaccgtatgt
     5461 ttaaagaagg agctgatatt gtaaaagtat ttcctgcagc ggttgttact ccacgttttt
     5521 tcaaggatat tcaagcacct ttaggtaagt taccactaat gggggttggt ggaatcagta
     5581 aggagaatgc gaaagagttt tttgaacaag gtgctagcta tttaggtctt ggttccggta
     5641 tgttcaacaa gcaagacatc gaagagctaa atgttaaaaa cttagcacgt tcgatgaaag
     5701 aattaattga ggctattaaa taggagtgag gactttggaa ctttatttga atgaggaact
     5761 cgtttttcga aatgttcaag caaaaacaga tagtgaagtc ttagcgtttt tagcaagcga
     5821 gatgtacaaa aaaaactacg tgaaggagga atatattcaa gcaatccaag aacgggaaaa
     5881 agaataccct actggattac catcaacacc accaggaatc gcaattcccc atgcaaacta
     5941 tgaaatggtc aataaaacaa ctttagcaat tgcgactctt aaagatccgg tcctatttca
     6001 taatatggaa gataacaatg agcagttacc aatccaaatt gtgatcatga tggcgattgg
     6061 ggaaccacat gggcaagttg aaatgttaca aaaaattgtt ggaatcatac aagatgagcc
     6121 actgcggcaa gaaatgatca gagcaggcaa tgatactgaa ttattagaac tattaaagaa
     6181 agcggttttt taatataagg aggaaatcat aatgaaacga attttagtag catgcggaaa
     6241 tggtatcgcc acttcaacag tagtagcaac aaaagttaga gagtatttgc aacaacatgg
     6301 cattgatgtt tcaacgactc aaacaaaatt gatggaagtt ccaggaaaag ttcaagacta
     6361 tgatctattg gtgacgactg gtcaatttga tggtcaaaca ggtggtgtac cagtaatcaa
     6421 agggatgccg attttaacag gtatcggtgc cgatcagaca atggaagaga ttttgaactt
     6481 actgaaataa tagctgatca aaggagctga aaaaatgaaa atcattatga acgtctttaa
     6541 cttctttatt gatgctggac caacagttat gttgccagtc atcatcacga ttattggttt
     6601 gatttttggg ttaaagattt ctcgtgcttt taaatcaggt ttgacattag gtatcggttt
     6661 tgccgggatc aagttgatat tggactttat gacgaccaat gttggaccag ctgccaaggc
     6721 gatggttgat cgaactggtg ttaaactaga tgctttggat gttggctggg gttcgattgc
     6781 ggctgttact tgggcatcgc caatcattcc aatcttgatt tttgccattc tattggtcaa
     6841 tatcgttttg ctgattttga aacgtacaca tacgctggat gttgatatct ggaactatca
     6901 ccatatggca atcgttggtg ttatagtgta ttttgtgact aagaatgtct tcttaggtgt
     6961 aggtgcatca gtagtgatgg caattgcgac ttttaagatt tcagactggt cgcaaccgat
     7021 ggtagaaagt ttcttcggaa tccctggtgt ttctttacca actgtttctg cgctttcatc
     7081 actggttatt gcttggccgt taaattggct gctggatcgt atcccgttat tcagaaaatc
     7141 aaaattcacc atcaaagatg cacaaaaata tttgggattt tttggtgatt ctatgatcat
     7201 ggggttagtt attgggatgg tgattggtgt tttagccggc tatgatatca aagcggtttt
     7261 acaattaggt gtcagcatgt cagctgtttt ggttttgatt cctaagatga cttcattatt
     7321 tatggaagga ttgatgccaa tttcagatgc tgcacaaaaa tggtcacaaa aaaaattcaa
     7381 aggtacacgt ttatttatcg gattggatgc tgcagttgtt gtaggtaatc cagatgttat
     7441 cacaacagca ttgatcatta ttccactaac gattgcatta gctttagtat tgccaggaaa
     7501 ccgtgtgtta ccattcgcag atttagcggt tgttcctttc cgcgtggcaa tggtcgttgc
     7561 cttgactcgt ggtaatttat taaaaaatat cattattggc ttagttgtga cagcttcatt
     7621 gctatggtgt gggtcagcta ctgcgccaat gctgacagca attgccaaat cggttggtat
     7681 taacttagga acttcttcaa tgttaatctc atcatttgct gcgacttcca tgatccaaag
     7741 ttatttggta tttatcgcat ttgcttacaa accaatcatc ggtattccag tgttgttatt
     7801 agcctttgcg ggcgcatggt attacttcga tggtatcaaa aaagttaatc atgaaatgga
     7861 tgaaccattg aaggcacaat agtaggaggg tttaaattga aaatttcaga tgtcacagtg
     7921 tataaagtaa aaccaagatg gatttttgtt aagatctcga ctgacgaagg aatcgaaggc
     7981 tggggagaaa tgatctcagg aactaaaaca gaaacggttg ttgctggagc ttatgagata
     8041 ggaaaccggc tgattggacg taatccgttt gaaatcgagc gcctcttcca agaaatgcat
     8101 cgatctttct tccgaggcgg tcctattaat ggaaccatcg tttcgggttt agaaatggct
     8161 ctgtgggata tcaaaggaaa agcgtttaat gttccggttt atgaattatt gggcggtgca
     8221 gcacgtgata aaatcaaggt ttattcttgg attggtggag atcgaccaag tgaagttgca
     8281 gaacaagcac aagatcggtt tgatcggggc tttacggccg tcaagatgaa cgcgacagaa
     8341 gagctgcatt atatcgattc ctaccaaaaa gttgatgaag tggtggaacg agttgcgtct
     8401 attcgcgaac gttttggtga tgcaatggat attggtgttg attttcatgg acgggtccat
     8461 aaaccgatgg caaaagtgct agcaaaagct ttggagccat tccatccaat gtttttggaa
     8521 gaagtcgttt tacctgaaaa tgaagaacac tttaaagaag tagctgattc tgtggctgta
     8581 ccattagcta ctggtgaacg cttgtacact cgttggcaat tcaagaacct ctttaaacaa
     8641 ggtactgttg atattatcca accagatgtg gctctttgtg ggggaatctt ggaaacccgt
     8701 aaaatcgcag cgatggcgga agcttacgac atggctgttg cgcctcatgc gccatatggt
     8761 cctgttgcac tagcagcaac tctgcaagta gactcatgta cgccaaacgt ctttatccaa
     8821 gaacaaagct tagggatcca ctataacaaa ggttttgatc tgttagattt tgtgaagaac
     8881 aaagaagttt tccaatataa agacggctac gtagatctac cttcaaaacc aggtctgggc
     8941 ttggaaatgg atgaagacaa aatcaaagaa attgcacaag aaggtttgat ttggaccaat
     9001 ccgcaatgga aaaattatga cggtacgatc gctgagtggt gataaatgaa aataaaggat
     9061 gatgaaagat ggcagaagca cttatgatca cagttttttt aggactgctg attattttgc
     9121 aaataatcga tcaaaagaaa attatcgtgt ttgctcagag tagtcctgta aggaagagtt
     9181 tgagtatttt actgacagtc gccatcctgt tgattttctg gccggaacat ctgactgatc
     9241 agattaaact ggttgctttt gcttctgtca ttttgatcaa tggttttttg aaggagggtc
     9301 tagcaaaaga ttctgttgtt agatttggag tattgaacgg taactttaat cgatatcctc
     9361 aagtccaagt agaaaaacta ggtgacgata aatcatttat ttcattttac aaaagcaaga
     9421 ataattcttt ttcaatgatt tttgataaag atcaaactga aatgatggat gtctttcaag
     9481 aacttgatat ccattccaaa attactttag atcaaagttc ggaagaaaaa agagcagttg
     9541 taaaaaaaga taatgaatga ttctactacc gaaattatga tttctttatc acgaataatg
     9601 aaacttgttt taatgaatca tattgattga agtatacaat gtatattaag ttttggaaag
     9661 aaatatattg gtacaagata tagcaggaat cgattactcg atccctgctt tttttaactt
     9721 cctttgcagc aaaaaaagct cgtaatctaa gagatgatgg atagttttta gaatatatat
     9781 attttcttca attccagaaa aaaatgacca agctagtatg ctaaaaaagt acgatgcttc
     9841 tatgatcttt tttccgtctt tattaacaga tgttttaatt gatcaacgca tattgtaatc
     9901 taccctaatt tccatagttc ttcaccactt ttaaaaatag taaaacccct acaagtatgc
     9961 tatagtaaat tgctaaagaa aaaccaataa ggtgggagat gtgtgcgcga tcaaacaagc
    10021 ggaaaaatga ttcgttttta ttatacaagt ttgcattttc tttattggat gatgttttgt
    10081 tctgtctatg ggtttgcgac attgtttttg atttatttca agattgatcc agctaaaatc
    10141 ggagggatct tagcattggt caatattgtt tcaacggtgc ttcagccttt tgtcagtcaa
    10201 gtgatcatca agaaaagaaa gatcccactg atcaccgtgc tgaagacgat gatagcagga
    10261 ttaggagtga ttctcttagc tggggtattg attccgaatt tgccgatcat tacttatatc
    10321 ttgggtggga ttttagtagt ttctatgcaa tcatttctca atgcgctggc atttgagtat
    10381 tcaaataccg gctacaggat caactttggc ttttcaagag ctggtggttc attttcttat
    10441 gctgtaactt cttttttact gggacagctg ttagcgattt attctgccgc gattttgcca
    10501 atcttggtta tagttgaagc agtgatattt ttcgttattt tgcatctgct gccggatgta
    10561 aaaaatcaag aagtttttcc tatgacaaaa gaacaggatc gctcagtttc tttgagaaaa
    10621 aaatatcctt tcctgatttt gttattttta ggtttcagtt ttctttttac tttccacacc
    10681 atgatcaaca gctttttggc acagatcttt gaaaatatcc acggcggcag taaagaagtt
    10741 ggcatcgcat tgatgatcgc cgctttatgc gaattaccgg gaatgatctt ttttgaacag
    10801 cttgtcaaaa agaaatccag caaattttgg ctgtctgttg ccagtatttc atttgctgtc
    10861 agaggattca tgatgctgct ggccagttcg atcctgatga tggaaatgac gcatctccta
    10921 caagccttca gtttcgcttt gtttattcca gcttcggctt atttgttcaa tcaatttctg
    10981 gcggatgatg atcgtgtatt tggacaaacg atgatcatca tcgccacaac tcttggtggg
    11041 gtgataggaa atgtcttggg cggcagtctg ctgcagcatt tcggtgtatg ggagatgctg
    11101 attttcggta ctggttttgc ggtgctaggt gctttattaa ctattttggg cattcgaaaa
    11161 ataccgaaga caggaaacga tagtgattct cttgttgagt agcataaaaa tacttagata
    11221 ctagttttat ataaataaca tatagtgatt attggcgtaa ttacaaaaaa cgagaataac
    11281 ttaagtaagc atttctaaat aattgagaaa cctcatctaa aaagataaga aaatatccct
    11341 aataaaactt ctgtaacatt ttagataact gattgtaatc tgaataataa actataagga
    11401 gacggttaga cggtctcctt tttttgcgta ttataattct agaattgttg gaaattgata
    11461 ataattaaca agaaatatta gacaggaact tcgaactgct tatcacattc ttattattat
    11521 atatttgcgt aagtagtcct ggtagtctca atgcactatt ctatcttgaa actaagaagt
    11581 tatagaagga gcaatgatag aaagcacgag aatacaaata gcgattaaat atttttctag
    11641 acaaccttag ttaattcaca cagatatatt ttaggtacag tcttacagat atagagttag
    11701 tgctgtgtga atcctgatgt tcgcagtgca taggagtcaa gtaagattct agccagtata
    11761 gaaagtggaa gtctggagcg tacctcataa tctgggaaga aggagccttc tgatttaaag
    11821 ccaacaaaag gaatctcgat atgttccatc aaacttgaat gacgattagc agtaagcaaa
    11881 attgtcccga tctcagcttt ataacagttt tcagctgcgg tagtcaattc ctttgtttca
    11941 ccgtttagtg aaacaaagat cacgatgtca tctttggata attttctact aatgtttttt
    12001 atgatattgg gatcagtatg catctcacaa tatttaccgg taagttgaaa tttgaccatc
    12061 atttcctgag caatcaattc tgaaaaacct cgggcaaaaa tcatgattct tctagcagcc
    12121 ttgattcgtt gaatagcatc ttcaatattc ccgctttcta ccatattgat ggtgcggatt
    12181 acctcttgct catttttaag aatagcagtc tttattcctt catcaacttt atcaagagcg
    12241 gcaaaattga tatttgtatt ttctttttct tttaacgaat gtttaaaaga cgtaaacccc
    12301 tcatatcctt ttttcttcat agtacgaacg acggttgctg ttgaaacgtt ggcatattca
    12361 cttagtttta caatggaata ggaggggatt tcgagtatgt gactttgaat aaaatcccag
    12421 agatatttct ccgattcact taattttgta tccatatttt gcctccgatt agttaggata
    12481 attctattgt aaagcgggag tttcctttac ttcaagttta tttaatatat tttgtaaaag
    12541 ttttcaatga atttagtcat ttatgaaaac atttacattc gatcttgata cataatgaat
    12601 atgtaagaaa ggaggagcgt aaatgtcagt ttatacatgt actttaaact tggcaatcga
    12661 cctttttatc gaaacagatg aaatgaaacc atcccaagtc aatcgaacaa acgatgatga
    12721 tatacaagct aatggaaaag gtgtgaatgt ttcattgatc atgaagcaat taggtatcga
    12781 tagtacagct ttaggattta aagcaggttt taccgggaat tatattgagg attatttgca
    12841 tcaaaaagat attagcactg attttattag cgtaccggga atgactagaa tcaacgtttt
    12901 tacacaagtt aataaaacca atgaagaatt caaattggtt aataaaggac ctgagattcc
    12961 tcaggaacaa atcaaaactt ttctaaatca aatacaaaag ctgcaagctg acgattattt
    13021 gtgtgtttct ggcagtttgc cgcgaggggt ctcacccgaa attcttattg atatcagtcg
    13081 tatttgtttt gaaaataagg tccatctgat tattgattca agctatccgg aaataattga
    13141 ttgtctgccg tatcagccgt tcttattaaa accaaacgag gaggaattgc aatcttggta
    13201 taatgtcgag cttagtgatc gtgaaggata tattcattat gctgaacgct tgattgccga
    13261 tggagcgaaa aatgttctcc tttctctagg aggagaggga ggaatatttt taaataagga
    13321 tatcgtttat tctgggaatt ctcctaaagg taaagtagtc aatacagcgt gtgctggtga
    13381 cactttgttg ggaacatttc ttgcaggata tttaaaaggt gaacaggtag atattaattt
    13441 gaagaaaagt ttagcagctg gaagttcaac cgcttttcgt aaaggattaa cagatttttc
    13501 ggatgttgca gagttggaaa aacaaatcga aatcaaagag ggagcgagta aaaaatgaca
    13561 acttacaaaa ttgttgccgc aacaggatgt ccaacaggaa ttgcccacac ttatatggct
    13621 caagaagcat tggagcaggc agctaagcgc aaaggggtat ctatcaaagt tgaaacccat
    13681 ggtcaagtag ggatagagaa tgaattaaca ccagaagaaa taaaaaacgc tgacgctgta
    13741 atcattgcag ctgacaaaga tgttcaagca gaacgtttcg caggtaagcg tgtaatcgat
    13801 gtgcctgtca gcaaggggat taaagatgct gaccaattga tcgaagatgc gttggctggt
    13861 aaaggttcaa tcactgcaga aaacaaaaca gttgacgctg ctggtgagaa agcggagatg
    13921 ggggttgcag caggcattgg tcacagtatt tataaaaatc tcatgaacgg tgtttctcat
    13981 atgctgccgt ttgtagtggc aggtggggta attattgcaa tttcgtttgc catctggggt
    14041 gtgtactctt ttgatcccaa tgatcctact tacaatccga ctgccgctat gttaaagggt
    14101 gttggtgatg cggcaatggg aatgatggtt cctattttag cagcttatat cgctgaagga
    14161 atcgctaaac gtcctggttt ggttgttggg tttgttggcg gactgttagc caattctggc
    14221 ggagccggtt tcttaggcgg aatttttgca ggtttcttag caggctactt tatcttacta
    14281 ttgcaaaagg tcatgaaagg cttgcctaaa caattggatg gtctgaaggc gatcttctta
    14341 tatccggtga tcggtgtctt gtttgttggt gtagtcatgt ctttattagc aaatccaatg
    14401 gaagcagtca accaaggaat gatgaatttc ttggcaggat tccaaaattc taatccatta
    14461 attttaggga ttatcgttgg ctgtatgtca gcatttgata tgggaggtcc agtcaataaa
    14521 gcggcttatg ttacagggac tgcgttgttg gcacaaggta accatacatt tatggcaggg
    14581 gtttcggcag cttgtatcgc gcccccattg attactggat ttgccgcact gttttttgga
    14641 aaatatttcg acacgaatga tcgcaacgct ggtttagtca actttatctt gggctcaacg
    14701 cacattaccg aaggagcaat tccatttgcg gctaaagatc cgctgcgtgt tctgccaact
    14761 atgatgctag gttcatcaat tgctgctgta ctgacatata tgtttggtgt ccaagttccc
    14821 gcacctcatg gaggttttct agtattacct gttgttactg gtaaggttgc atgggtaggg
    14881 tcgattttac ttggttcaat cgttggcgga gtaattttag ggttgatcca aaaacgtagt
    14941 gtagaaaaac gtgaagcaga agcagctgct gttgaaatga aagaagcaac caacaactaa
    15001 aggttattct agaaagaagg atttcaaatg actttgatca aaacaaataa agtttttatg
    15061 aataaaaatt ttacgacgca agatgaggta atgaacttcc ttgcagatca gtcaattgtc
    15121 ttgagcatta gtgaagaccg agatgcagtt tacgaaaaat tgatggaaag agaagaagaa
    15181 agcacgactg gtatgatgga tggttttgcc atccctcatg ccaaagatgc tagtatcaag
    15241 gatgccgata ttgtgattgt tcggttaaac ggtaaggtcg attggaaaag tttagatgga
    15301 gaaggaacgg attttgtaat cgctctgttt attccagatt cagaggctgg aacaacccat
    15361 ctaaaatttt tatctcaggt agccagactt ttgatgcaca aagatgtgac agaaggtctg
    15421 aaacaagccc agtctgctga agaaatagct gaacttttaa accaaaaatt agaagaaaac
    15481 taaaaaggaa gtagtgaaat catgccttta acagaaaaaa agaaaactta tattcgtcaa
    15541 ctaagtaatg aaagtggcat catcagtgcg ttagcaatcg atcaacgtgg tgccttgaaa
    15601 aaaatgatcg gtaaatttaa aaataaatca gcaaccgatg aacaaatcat caatttcaaa
    15661 aaaatcattt cttctgaatt gactccatat gcttcagcaa tcttattaga tccagaatat
    15721 gggttgccgg cagccgaagt tcgcgataaa aatgctggtc tgttgttggc ctatgaaaaa
    15781 actggttacg atgcttcaac tccaggacgt ttaccggata ttttatctat ttggtctgta
    15841 aaacgtctaa aggagcaagg ggccgatgct tgtaagttct tgttatacta cgatgtagat
    15901 gaatctgatg acatcaatga ccaaaagaag gcgtttatgg agcggattgg ttctgaatgt
    15961 gttgctgaag atttgccatt tttcttagaa ttggtttctt atgatgccaa cgatcctgat
    16021 acttcttcga aggaatatgc cttgaaaaaa ccgcataaag taaatgaaat gatgaaggaa
    16081 ttttctaagc ctcgctatgg agttgacgtt ttgaaagtgg aagttcctgt taatatgaac
    16141 tatgtggatg gttacactaa aaatgaagtt gcttatacca aagaagaagc tgctgcttat
    16201 ttcaaagaac aatccgagac taccaacctg ccatttattt tcttgagcgc aggagtaact
    16261 gctgaattgt tccaagaaac cttgaaattt gcaaaagagg ctggctctac attcaatggg
    16321 gtactctgtg ggcgcgcaac ttgggcgaat ggtgtagaac cattcgttac taaaggtgaa
    16381 tatgctgcca aagaatggat gcagacacaa ggacgcaaga acatcgaaga gctgaataat
    16441 gttttgaaag aaacagcaac agctattaaa ttgtaagaaa gctttaacta taaccttaag
    16501 tttgtaacct aatgattttt aataatcaat ttgagctttt aaataaagaa ttggaagcgt
    16561 cccgacgctt cctttttcgt ttgtttttaa ggcatgatag gttctatgtt gtcgttcaat
    16621 aagcgcaaga aggtcagcat ttttttctta gagtatgcaa aattgctttt ctacccaatg
    16681 atttaaaacg gagaggcacc cttggatatg gtagatggtg aagtaacgca tttcttctgt
    16741 gatcacggtt tttttgtctt cttgtagcca gatttttaaa taattttctt cgatcagatt
    16801 atttaatctc ttttgaaatt ggatatctcc gtggttgatg acgaataact tgtatacgaa
    16861 cgtattcgta tagacatagt cgatcaattg agcgtataga tcctcgtagc tatgtgtcgt
    16921 atcgtaggaa atgatttcat ccaagtcttg gaaaaagcta ttttccagtt tttcataaag
    16981 atcatagata tccgtataat gtgtataaaa tgtcgaacgg tgaagatcag cagtttcggt
    17041 cagttcacgg atggtgatgt tttgaatttt tttatctact aataattttg ataatgcttc
    17101 tgctaacgct ttttctgttt ttttgacgcg gcgatcctct tttttcattt tgcagcctcc
    17161 taatcaacat aatcaaggaa ttgtgtcggt tatccgacaa tttcaccgct atctgaacat
    17221 agataaccga caccttgtct attataatat aagtatagca tatcaatatt taaagaatag
    17281 gagaataagt gatggaatta gcctttaagc aactggaaaa gaaagactac aaaaaagcca
    17341 ttcaattcgc gattaaaggt atgcactttg attggtatac caagaacaaa ctccttttga
    17401 atctttatgg aagatacttt tggtatctag aattgaatcg cgcgacacaa atcattgctt
    17461 tatatgcgga tgatgagttg gcaggtgttt tattagcaga catcaaagga aaatcaaaaa
    17521 agcatcattc atttagtcaa aagttatatg ttaaactatt tgatttctta caaaatgcat
    17581 ttgtgaaaga cagcaaaggc gggtatgacg acaccaatga cgaattgctt tcagactatt
    17641 taaaaaaaca ttccccagat ggagaaattg tctttttagc tgccaaccct gatttaaaga
    17701 tcaaggggat cggtagtaaa ttattaaaag aatttgaacg ccgagagcaa ggaaaagaag
    17761 tatttctgtt cacggatagc ggctgtacgt atcaatttta tgaacgacgc ggtttcgaac
    17821 ggaaaaaaga aaaagataac gtcatgaaga tccttaataa agaagtagat ctccgctgta
    17881 tgttatatag taagatcatt cagtaatgtt ttttactatc agaaagcaga agtaccacga
    17941 aggtcaatgg aaaaagcgag taattgatcc atttagatga agataggtgt agtctcaaag
    18001 atattagctg gcaaagatat ccccagttaa aaatagattt ctgtatccaa caataaaaat
    18061 atttttgtat ttattagtat atatacttta caaaaatttg tatatatact aatttttttg
    18121 taggatcaat gaaatgaggg gaaaatatgc caaaatataa agaaatagcc ttggctctaa
    18181 aagagaggat cgctgaggga aaatatcagg aaggttctaa attacctgag caagaaagtc
    18241 tggctagaga gtttaaaaca agccgtgtta ctatccgcaa agctatacaa ctccttattg
    18301 atgaaggttt gttatatacg agacgtggat ctggtacttt tatacgaagc aatatcagac
    18361 aggacaatga gaatgtcact caagtaaata atgtatttgg cacttcctca caagaaaata
    18421 caaatgtgac aagtaaaata atccgttttg atgtccggtt tccgacagat tatgaaaggc
    18481 ggctttctgc aacctacttc cgatcccctt cctgcaaatc atgggta
//
LOCUS       DACKAC010000054        17715 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.055, whole genome shotgun sequence.
ACCESSION   DACKAC010000054 DACKAC010000000
VERSION     DACKAC010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17715
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.055"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            4..816
                     /locus_tag="I0B46_001974"
     CDS             4..816
                     /locus_tag="I0B46_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAR2084252.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="I0B46_001975"
     CDS             813..1613
                     /locus_tag="I0B46_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAR2084253.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="I0B46_001976"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="I0B46_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAR2084254.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="I0B46_001977"
     CDS             3110..4819
                     /locus_tag="I0B46_001977"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAR2084255.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5031..9383
                     /locus_tag="I0B46_001978"
     CDS             5031..9383
                     /locus_tag="I0B46_001978"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAR2084256.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9527..10000
                     /gene="rimP"
                     /locus_tag="I0B46_001979"
     CDS             9527..10000
                     /gene="rimP"
                     /locus_tag="I0B46_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAR2084257.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10024..11199
                     /gene="nusA"
                     /locus_tag="I0B46_001980"
     CDS             10024..11199
                     /gene="nusA"
                     /locus_tag="I0B46_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAR2084258.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11221..11514
                     /locus_tag="I0B46_001981"
     CDS             11221..11514
                     /locus_tag="I0B46_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAR2084259.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11511..11822
                     /locus_tag="I0B46_001982"
     CDS             11511..11822
                     /locus_tag="I0B46_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAR2084260.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11835..14141
                     /gene="infB"
                     /locus_tag="I0B46_001983"
     CDS             11835..14141
                     /gene="infB"
                     /locus_tag="I0B46_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAR2084261.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14168..14515
                     /gene="rbfA"
                     /locus_tag="I0B46_001984"
     CDS             14168..14515
                     /gene="rbfA"
                     /locus_tag="I0B46_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAR2084262.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14623..14844)
                     /locus_tag="I0B46_001985"
     CDS             complement(14623..14844)
                     /locus_tag="I0B46_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAR2084263.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
     gene            15292..17655
                     /locus_tag="I0B46_001986"
     CDS             15292..17655
                     /locus_tag="I0B46_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HAR2084264.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
BASE COUNT         6037 a         3147 c         3902 g         4629 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag tagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtgatc acagcagctg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcggtagta ggagtttacg agctgttccg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtatcg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattca atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg tccgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcgtgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2521 ccaggtaaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcat attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct ataccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gatcatcgga gacggcggcg caatgggcgg
     3721 gaaagactca aaggaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgtggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccaaaacg cgatttcact gtgctttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaac ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tattcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaattg
     4501 gccaaaagga attgcgccat ttgatctgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaagc ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gcgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4861 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     4921 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     4981 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5041 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5101 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5161 gggaatttac gctacatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5221 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5281 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5341 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5401 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5461 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5521 aagtggatga gcagcaagcg gaacgtgtat tgaaactttt tgaagaacgc aaacaagaac
     5581 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5641 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccagctagga cgaaatattc
     5701 cagccgatga accaacgaca ccaatgatca gtatcgttga agaagaacga agagtcacac
     5761 ttgaggggta cgtattcgac aaagaagtca gagaactacg ttcaaaacgc aagatcttga
     5821 ctttgaaaat cacagattat acttcatcat ttatcgtcaa aaaattctcg aacggcgaaa
     5881 aagatgaaca agtatttgat gcgatcagtg tcggtagctg gttaaaggta cgaggaagta
     5941 ttcaagaaga tacgtttgtc cgtgatttgg tcatgaatgc gcaagatatc atagaagtca
     6001 aacatacacc tcgcaaagat tatgctcctg aaggggaaaa acgagtagag cttcatgttc
     6061 atagcaatat gagtacgatg gatgcaacaa acagcatctc tgatcttgtg gcacaagcag
     6121 gaaaatgggg acaccgagct atcgccatta cagaccatgg tggtgcgcaa gcctttccgg
     6181 aagcacatag tgctggaaaa aaagcaggtg tgaaaatctt atacggcgtg gaagcgaatg
     6241 tcgtagatga tggtgtccca attgcttata atgatgcaca tgaagcattg agtgaagcca
     6301 catacgtcgt atttgacgtg gaaacgacag gattgtctgc agtatatgac acgatcatcg
     6361 agctagccgc agtcaaaatg tataaaggaa atgtgatcga aagctttgat gaatttatcg
     6421 atccaggaca tccattgtca cgaacaacgg tagatctgac tggtatcacg gatgaaatgg
     6481 ttcgagggtc aaagtcggaa gaagaagtat tgcggatgtt ccttgaattt tcaaaagata
     6541 caattttggt agcccacaac gcggcttttg atatggggtt cttgaatact agctacgcaa
     6601 ggtatggtat tcctgaagcg gccaatccag tcattgatac gttggaatta gctagatatc
     6661 tgtatcctca attcaaacgt ttcggattag gtgtgttatc taaaaagttt ggtgttagct
     6721 tggaacagca tcaccgagca atctatgatg ctgaagcaac tggtcactta gcatggatct
     6781 ttgtgaaaga ggcaatggac aatcataaca tgctttatca tgatcagcta aatgagcata
     6841 tcggagaagg agattcttac aaaagggctc gacctttcca tgtaacgatt ttagcaaaaa
     6901 atcaggcagg actgaaagat ttgttcaaac tgatttcaat gtcgaatgtc gaatacttcg
     6961 aacgcgtccc acgaatacca cgctcccaat tgaaaaagat gcgtgaaaat ctattgatcg
     7021 gctcagcttg tgataaggga gaaatattcg aagccatgat gcaaaaaggg gtagaagagg
     7081 caagaaaccg agcaaaattc tatgattata ttgaagttat gccaaaagcg gtctatgcgc
     7141 ctttgatcga gcaagaattg gtaaaaaatg aacatgattt ggaagaaatt atccaaaatt
     7201 tggtagaaat cggaaaaagt ttggataaaa tcgttgttgc aacaggtaat gtacattatc
     7261 tgaacgaaga agatgccatt taccgaaaaa ttttgatcaa ttctatgggc ggagcgaatc
     7321 cgttgaatcg tcatagtttg ccggatgtcc atttccgaac aaccgatgag atgctaactg
     7381 catttcactt tttaggggaa gaaacagcga aagagatcgt agtagaaaat acgaataaaa
     7441 tcgcagatat ctgcgaagaa gtgattccag tcaaagatga actttataca ccgaagattc
     7501 caggttcaga agatgaaata tcagaattga gttacacaaa agcaaaacaa atgtacggag
     7561 atccgttacc tgagatcatt caaaaacgat tagaaaaaga gttgaattct attaatggta
     7621 atggcttctc ggttatttat ctgatcgccc aaaaactagt gcataaaagt aatgaagatg
     7681 gctatctagt aggttcacgt ggctctgttg gctctagctt tgttgcgacg atgacaggaa
     7741 tcactgaagt taatccatta gcccctcatt attattgtcc agagtgccaa tattccgagt
     7801 tttttgaaga tggtacgtat ggttctggat ttgacatgcc agaaaaacaa tgtccaaaat
     7861 gtggtgcacg cttaaacaaa gacggtcatg atattccatt tgagacattt ttaggattcc
     7921 atggagacaa agtgcccgat attgatttga acttctcagg tgattatcaa gcagaagccc
     7981 ataactatac gaaagtattg tttggcgaag actatgttta ccgtgccgga acgattggta
     8041 cagtcgccga caaaacagca tatggctatg taaaaggata cgaaagggac aataatctgc
     8101 agtttcgaag tgcagaagtc gacagattag caaaaggcgc tactggagtc aaacggacaa
     8161 ccggccagca tccagggggg atcatcgtta ttcccgatta tatggatgtc tatgatttta
     8221 cgccgatcca atatccagca gacgatcaaa attcagaatg gaaaacgact cattttgatt
     8281 tccactcgat tcacgataat gtattgaaat tagatattct agggcatgat gacccaactg
     8341 ttattcggat gcttcaagat ttatcaggga tcgacccaca aacgatccct acggatgacc
     8401 cggaagtgat gcggatattt gccggtcctg aagtacttgg agtaagtcag gaacaaattt
     8461 attccaaaac agggacatta ggtattccag aattcggcac tcgttttgtt cgagggatgt
     8521 tagaagaaac gcatcctaca acttttgcag aactactgca aatttccgga ctatctcatg
     8581 gtacagacgt atggttagga aatgcggaag aattgattcg acgaggagat gcgacactgg
     8641 cggaagtaat cggctgtcgt gatgatatta tggtttattt gatccatgct ggattagata
     8701 gcgggatggc atttaaaatc atggaaactg ttcgtaaagg acaatggaac aagatccctg
     8761 atgaattgcg agaaacctat ctatcggcga tgaaagaaaa taatgtgccc gactggtata
     8821 tcgattcttg ttcaaaaatc aaatatatgt ttccgaaagc ccatgctgca gcttacgtat
     8881 tgatggctct tcgtgtcgct tatttcaagg tttatttccc tattttgtat tactgtgcat
     8941 acttttctgt acgtgcagac gactttgatc tagtctccat gtgcaaagga aaagatgctg
     9001 tgaaacaagc aatgaaagaa atcacggaca agggattaga tgcttccgta aaagaaaaga
     9061 atcaactgac agtgctggag ttagccaatg aaatgctgga acgaggattc aaatttggaa
     9121 tgattgattt gtataaatcg gatgccgtta actttgttat tgaaggtgat acgttgatcg
     9181 caccatttag agctgtccca agtttgggaa ccaacgtggc gaaacaaatc gttgaagcaa
     9241 gaaaagacgg tccattttta tccaaagaag atcttgccac ccgcggtaaa gtttcaaaaa
     9301 cattgatcga atatatgaac gacaatggcg tattgaaaga tctaccagat gaaaatcaat
     9361 tatcgttatt cgatatgcta taagcatctg ttttcctgtc tgcttgtaat tttaagcaga
     9421 gtatggtata gttcttatag gtaatgatac taactgtgag tgagcggatt tttcgctcac
     9481 tctttttaat ggaattacgc acttaatttt taaggaggcg aaacttttga gtagcgtcgt
     9541 tgaaacagtc acagaaatgg tgacaccgat tttagatgaa caaaattttg aacttgtcga
     9601 agtcgaattt gtaaaagaag gaaaaagctg gttcttacga gtatttattg acaaagaagg
     9661 cggtatcgat atcgaagaat gcgcctttgt cagtgagaaa ctgagtgaaa aacttgatgc
     9721 agcagatcca gatccgattc ctcaagctta tttcctggag gtatcctccc cgggagcaga
     9781 gcggccattg aaaaaagaaa gcgactatga aaaggcactt ggtgattatg tccatgtctc
     9841 cttataccaa ccagtagatg gcgaaaaaca atatgaagga tttcttcaat catttgatgc
     9901 tgaacagctt acattgaaaa tacgcataaa aacacgagaa aaagaattga cttttgaccg
     9961 aaaaaatatt gcaaaagctc gcttagctat ccaattttaa tcacggagag gaatcagaaa
    10021 aaaatgagta aagaaatgtt aaatgcgtta gacgctttag aagctgaaaa agggatctca
    10081 aaagaaatcg tgatcgatgc tttagaagca gcattggttt ctgcatacaa acgtcactat
    10141 ggccaagcac aaaacgtcga agtggaattt gatcagaaaa aaggaaagat ccatgtctat
    10201 gcggtaaaag aagtaacgga agaagtgatg gattcacagt tggaagtctc attgaaagat
    10261 gcacttttaa tcaatccagc atatgagatc ggtgatacga tccgttttga agtaacacca
    10321 aaagattttg gacggatcgc tgcgcaaacg gctaaacaag ttattttaca gcgtgtacgt
    10381 gaagcagaaa gaacgattat ttataacgaa ttcagtgctt atgaaaaaga catcatgcaa
    10441 ggaatcgtcg aacgccagga taaacgttat atttatgtca atcttggtaa aattgaggca
    10501 gttctttcta aacaagatca aatgccaaat gaattttatc aaccacatga tcgaatcaaa
    10561 gtttatgttt cccgagtaga aaatacgtct aaaggtccgc aagtatttgt tagccgcagc
    10621 catccagacc tattgcgtcg cttatttgaa caagaagtac cagaagtcta tgatggaatc
    10681 gttgaaattg tcagcatcgc ccgtgaagct ggcgatcgtt caaaagtggc agttcgctca
    10741 aatgatccaa atatcgatgc agtcggtaca tgtgtcgggc caaagggaca acgagttcaa
    10801 gcaatcgtca atgaattaaa aggcgaaaac atggatatcg tcgaatggaa tgaagatcca
    10861 gctgtattca tcgcaaatgc attgaatcct gcacaagtca tggatgtgat cttcgatgag
    10921 acaaatccaa aggcctgcac agttgtcgtt ccagactacc aattatcgct agcaatcggt
    10981 aaacgcggac aaaatgcacg tttagctgct aaattgacta accataaaat cgatatcaaa
    11041 tcagaatctg acatgacgga attttatgaa aatcaagaac aacaaaaaga aacagaagaa
    11101 ctgcatgatg aagcaatcgt tcagtcagat ttaacagacg atgaatatga aacaattgca
    11161 ttcaacaatg aaacggtcga agaaaaacca gaagcttaac tttagtaagg aggcaaagag
    11221 atgaaacaaa ggaaaattcc tttacgcaaa tccgtggttt caggtgaaat gaaacctaaa
    11281 aaagagatgg tccgaatcac ccgttcaaaa gaaggggaag tatcgatcga tccaactgga
    11341 aaaatgcctg gacgaggagc gtatgtctca cttgagccgg aagaagtaca acaagcttgg
    11401 gataaaaaaa ttctcgatcg agtactcgaa acaacattaa cagatgagtt ttatcaagaa
    11461 cttcttgact atgtcactca ccaaaaagca cgtaaagagt tatttggaaa atgaatacag
    11521 ttaatcggca aaaagccatg aatctcatcg gtctagcgat gcgcgcggga aaactaatca
    11581 cgggtgaaga actgaccatt gctgaaatcc gcaaacaaaa agcaaaattg gtgtttgtgg
    11641 caaccgatgc aagtgagaat acaagaaaaa agataaaaga taagagttca tattatgaag
    11701 ttccttgctt tgagctattt tctgaagcag aaatcacgca gatgatcgga aaacctcgca
    11761 aggtattcgg aataatggat gccggatttg cgaagaagac caaggagcta attgaaggtt
    11821 aggaaggtga ttgcatgggc aacaaaagaa tctatgaatt agcaaaagaa ctcaaaaaat
    11881 ctagtaaaga cgtcgtggaa aaagcacaac aacttggcat cgatgtgaaa aaccatatgg
    11941 gtgcgattac tagcagtgat gagaagaaat tgcaacaagc gttcaaaaac ccaagcacga
    12001 aacaacaagc tgcacaacaa gcaagcagaa aaccagccaa tcaacagccg aacgatcaac
    12061 gtaaaaaaga aacaacgaac aatcaacaaa aaaataaaaa taaccgcagt tatcaagatc
    12121 gcggtcaagg gagcggtcaa gtgaatcaag gaaaaaaacc aacaacaaac caaaataata
    12181 cgggaaatac cagcagccaa aatcgtcaag gaacgagcca aggcggcaat caaaaccgta
    12241 ctaacaataa caacaaacca aacaataaca atcgcggcaa atttaataat aataaccgta
    12301 atcgttataa taaaaaaggt aaaaaaggca aacagcaaca atcaactaaa ccagctgtac
    12361 caccacgcaa attccgtgaa ttgccagatg tattagagta tactgaaggt atgaacgtag
    12421 cagatattgc gaaaaaaatt catcgtgagc cagcagaaat cattaaaaaa ttatttatgc
    12481 ttggtgtcat ggtcaatcaa aaccaagcac tggataaaga tacgattgaa ctgttagcga
    12541 cggactatgg catggaacca caagaaaaag tccaagttga cattgctgat atcgataaat
    12601 tcttcgaggc agaagaagtc gatccagata aattagtctc acgtccgcca gtagtaacga
    12661 ttatgggaca cgtagaccac gggaaaacaa ccttattgga tacattgcgc cattcacgtg
    12721 taacaagcgg cgaagccggc ggaatcacac agcatatcgg tgcctaccag atcgatattg
    12781 atggtaaacc aatcactttc ttggatacac caggacatgc ggcctttaca agtatgcgtg
    12841 cacgtggagc aagtatcaca gatatcacga tcttagttgt tgctgcagat gatggcgtca
    12901 tgccacaaac agttgaagca atcaaccatg ccaaagctgc aggcgttcca atcatcgtcg
    12961 ctgtaaataa aattgataaa ccaggtgcaa atcctcagca tgttatgcaa gaattaagtg
    13021 agtatgaatt aattcctgaa gcatggggtg gagaaacgat ttttgttgag atctcagcga
    13081 aattcggcca aaacatcgaa gaattgctgg aaatgatcct gttagtcgca gaagtagaag
    13141 atttaaaagc agatccaaca caacgcgcaa tcggaacagt gatcgaagca cgtcttgata
    13201 aaggtaaagg tcctgtgtca accttacttg tccaagaagg aacacttcac gttggtgacc
    13261 caatcgttgt cggtaatact tacggacgtg tccgtgtaat gacaaacgat cttggccgtc
    13321 gcgataaaga agcaggacct gcaacgccag tagaaatcac tggcttgaat gatgtacctc
    13381 aagcaggtga ccgtttcgta gtcttcgaag acgaaaagac agcacgtgca gcgggtgaag
    13441 aacgcgcaaa acgtgcatta ttggaaaacc gtgctgccac aagccgtgtg actttggaca
    13501 acttatttga aagcctgaaa gaaggcgaat tgaaagaagt taacgtaatc atcaaagcag
    13561 atgtacaagg ttcagcagaa gcactggctg catcattgaa gaagattgat gtggaaggtg
    13621 tccgtgtaaa aatcgtccat tcagctgtcg gagcaatcaa tgaaagtgat gtaaccttag
    13681 cagcagccag caacgctatt atcatcggtt tcaacgttcg tcctacaccg caggcaaaag
    13741 tgcaagctga tactgaagaa gtcgatattc gtcttcaccg tatcatctat aaagccatcg
    13801 aagaaatcga aactgcgatg aaagggatgc tagaccctga atttgaagaa aaaatcaccg
    13861 gtcaaatgat cgttcgtgaa accttcaaag tttctaaagt tggtacgatt gcaggtgcct
    13921 atgtaacaga aggatatatt cgtagagata gcggtgtccg tgtaatccgt gacggaatcg
    13981 taatctatga aggccaactt gcaagtctga aacgattcaa agatgacgtg aaagaagtca
    14041 aaatgggtta tgaatgtggt gcgatgattg aaaaattcaa tgacatcaaa gtagatgacg
    14101 taattgaagg atttatcatg gaagaagtca aaaatgacta aaaactgata aaaggaggcc
    14161 aatcattatg gctaactacc gtgatcgccg agtcggtcaa gaaatcttaa aagaagtcaa
    14221 tgatattttg cgcaaaaagg tccgtgaccc tcgtgtggag aacgtaacga tcacagatgt
    14281 gaaagttacg ggggacttac agcaagcaac gatctattac agcattttat ctgacttagc
    14341 atcagataag aaaaaagcac aagatggatt gaataaagca tcaggattga ttcgtcgaga
    14401 gttagggcac aatctaagta tttataagac acctgaattg ttctttgaac ttgatgaatc
    14461 agttgtctat gggaaccata tcgatgaatt gcttcgcgac ttgaacaaag actaatcaaa
    14521 aaatcccgca aatcctgtga aatcaggggt tgcgggattt ttttgttttc attaagacgt
    14581 attacctaag agttccatga ctttagtctg gtagcgtgtc agctaaccaa aaaagagaaa
    14641 aagaaaagta aaaaattcga ctttcttttt tctatagtat ataaattttt gcatatagat
    14701 aaagatcaag ataccgataa cggaagctag attgtaatct attttagtga cagtcaggta
    14761 tagccaagta actagaagat cagtggaacc tgcaccataa aagtttgagt ttgtcggtcc
    14821 gttgacagtt aaaagaaaaa tcacgttgaa gttattgata tttccgatta cgccgcttcg
    14881 atttggtcac gatccaaatt ttggataatc cctgtagaaa ttagcatagt agctgggata
    14941 ccaattcaca tattgacaaa gataacagag acttttacat aaaacggatc aatcaggaaa
    15001 ggaattgctc gatccagccc cagttttcca acaatgtgtt aactggccta gcgtcattca
    15061 gcaaattatg catgatgagg agcgaaatga actgcggaac agccattgtg ataacgacaa
    15121 gttttttgga tcaatgtaaa aaacgtcagc gaaaggaaaa atttcgtggt tttttatacc
    15181 tgttcatttg gctagatata aaatccaaac aaatgaacag aagtgcttat taggtttcaa
    15241 ttgggagtat ctgcgctaga ataagagtaa taatgaaaag gagtgttatg tatggattat
    15301 tctagtaaag aatactttga taaaatgacg gcttggtggc gtgctgctaa ttatctttct
    15361 gttgggcaac tttatcttaa agataacccc ttactgagac gtacactgaa accagaagat
    15421 gtcaaaaaac atccaatcgg tcattgggga acgattcctg gccaaaattt tatttatgtc
    15481 catttgaacc gagtcatcaa taaatatgat ttgaacatgt tttatatcga agggccagga
    15541 cacggcggac aagtcatggt atcgaatgct tatttagatg gaagttacac agagatctat
    15601 ccagaagtaa cggaagatga gacaggaatg caaaaattat tcaagcgatt ttcatttcct
    15661 ggaggcattg cttctcatgc agcaccagaa acaccaggtt ctatccatga aggcggagag
    15721 cttggatact ctctttcaca tgcagtcgga gctgtattag acaatcctga agtaatctct
    15781 gctgtcgtga ttggtgatgg tgaagcagaa accggtcctt tagctggaag ctggttctca
    15841 aatgtcttta tcaatccagt gatagatgga gctgttttac cgatccttca tttaaatggt
    15901 gcaaaaatag ccaacccaac gatcttggca cgtaaatcag atggagaatt agcaaattac
    15961 tttaacggac ttggctggga gcctttcttt attgaaggaa atgatccaga aaaattaaat
    16021 cctgttatgg ctgaaaaaat ggatcaagca atcgagaaaa tcaaatccat ccaaaaagaa
    16081 gctcgcctga agacagcaac agatgtcgtg atgccaaaat ggccagtatt gatcgtacgc
    16141 acaccaaaag gatggaccgg tccaaaagaa tgggacgggg aaccaattga aggaaccttc
    16201 cgagcacacc aagtaccgat tcctgtagat caagaacata tggatcatgc agatgcttta
    16261 ctccgttggc tgaaatccta tgaaccagaa aaattatttg atgcacaagg acgtatttta
    16321 gaagaaatca gagaaattgc gcctactggt gaccaaagaa tggcaaaaaa tcctatcacc
    16381 aatggtggaa tcgatccaag accgcttatt atgccagatt ggaaaaaata tactttgcaa
    16441 tttgaaaaac caggctccgt taaagcagaa gatatgaccg aacttggtaa attcgttcgc
    16501 gagatcattg agaaaaatcc agaaaatttc cgtatatttg gaccagatga aacaaaatca
    16561 aaccgattga atcaagtttt caaaacgaca aatcgccaat ggatggaaaa aatcgaacca
    16621 gaaaatgatg aatggctttc gccatctggt cgcgtcatcg acagtcaatt atcggaacac
    16681 caagatgaag ggttcttaga agggtacgtt ctgactggac ggcatggttt ttttgcaagt
    16741 tacgaatcat tcttgcgagt ggttgactct atgttgactc agcacttcaa atggatacgg
    16801 aaatcacatg acctttcttg gcgaaaagat tatccttcgc tgaatctcat tgcaagctca
    16861 acagtcttcc agcaagatca caatggatat agccaccaag atccaggaat attgacacat
    16921 ttggcagaaa agaaggcgga gttcatccgt gaatatctgc ctgcggatgc aaatacattg
    16981 ctcgcagtca tggataaagc attccgctcg tcagaaaaaa tcaatttgat catctcttcc
    17041 aaacatccgc gtgctcaatt ttactcagca gaagaagcag ccgtcttagt gaatgaaggc
    17101 ttgaaaatca ttgattgggc ttcgacagca aaagaggagg aacctgaatt ggtcattgct
    17161 gctgcaggaa cggaatctaa cttagaagca ttagcagctg taactttgct gctggaagaa
    17221 ttcccaaaac taaaaattcg gttcatcaat gtcgtagatt tattgaaatt acgtcatcca
    17281 agtcaagatc cgcgtgggtt gagcgatgaa gaatttgatc aatattttac aaaagacaaa
    17341 ccgatcttat ttgctttcca cggttatgaa acattggtcc gcacgatttt ctttgatcgt
    17401 cataaccatc atttaatgat tcatggctac aaagaaaatg gagatatcac gacaccattt
    17461 gacatgcgtg tagtaaatga acttgatcgt tatcatttgg caaaagacgc agctttaaaa
    17521 atcaaaggaa gtcaagcaga agattttgcg gaaaaaatgg atcagaaatt acaagaacat
    17581 caaaactata ttcgtgaaaa tggtattgat ctgccagaag tattggattg gaaatggaaa
    17641 aatcttgacc aataaaatga taaaatcgat cccgcaaaac agaatttttt acattgtatt
    17701 atttgtcaaa ttaag
//
LOCUS       DACKAC010000055        17689 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.056, whole genome shotgun sequence.
ACCESSION   DACKAC010000055 DACKAC010000000
VERSION     DACKAC010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.056"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(137..1717)
                     /locus_tag="I0B46_001987"
     CDS             complement(137..1717)
                     /locus_tag="I0B46_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAR2084265.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2118..3494)
                     /locus_tag="I0B46_001988"
     CDS             complement(2118..3494)
                     /locus_tag="I0B46_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAR2084266.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(3622..4740)
                     /locus_tag="I0B46_001989"
     CDS             complement(3622..4740)
                     /locus_tag="I0B46_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAR2084267.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            4882..5316
                     /locus_tag="I0B46_001990"
     CDS             4882..5316
                     /locus_tag="I0B46_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAR2084268.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5394..5927)
                     /locus_tag="I0B46_001991"
     CDS             complement(5394..5927)
                     /locus_tag="I0B46_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAR2084269.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6021..7199)
                     /gene="mutY"
                     /locus_tag="I0B46_001992"
     CDS             complement(6021..7199)
                     /gene="mutY"
                     /locus_tag="I0B46_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAR2084270.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7192..7989)
                     /gene="recX"
                     /locus_tag="I0B46_001993"
     CDS             complement(7192..7989)
                     /gene="recX"
                     /locus_tag="I0B46_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAR2084271.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8096..9478
                     /gene="rlmD"
                     /locus_tag="I0B46_001994"
     CDS             8096..9478
                     /gene="rlmD"
                     /locus_tag="I0B46_001994"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAR2084272.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(9652..10230)
                     /locus_tag="I0B46_001995"
     CDS             complement(9652..10230)
                     /locus_tag="I0B46_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAR2084273.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            10552..11022
                     /locus_tag="I0B46_001996"
     CDS             10552..11022
                     /locus_tag="I0B46_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAR2084274.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGEYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(11130..12356)
                     /locus_tag="I0B46_001997"
     CDS             complement(11130..12356)
                     /locus_tag="I0B46_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAR2084275.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(13110..13310)
                     /locus_tag="I0B46_001998"
     CDS             complement(13110..13310)
                     /locus_tag="I0B46_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAR2084276.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(13444..14052)
                     /locus_tag="I0B46_001999"
     CDS             complement(13444..14052)
                     /locus_tag="I0B46_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAR2084277.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNAAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(14137..14607)
                     /locus_tag="I0B46_002000"
     CDS             complement(14137..14607)
                     /locus_tag="I0B46_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAR2084278.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(14655..15569)
                     /locus_tag="I0B46_002001"
     CDS             complement(14655..15569)
                     /locus_tag="I0B46_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAR2084279.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKNVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            15715..16518
                     /locus_tag="I0B46_002002"
     CDS             15715..16518
                     /locus_tag="I0B46_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAR2084280.1"
                     /translation="MTTKQLIISSFIRFKDLKETRNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKELR
                     SFLSKASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            16696..17643
                     /locus_tag="I0B46_002003"
     CDS             16696..17643
                     /locus_tag="I0B46_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAR2084281.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
BASE COUNT         5365 a         3543 c         2911 g         5870 t
ORIGIN      
        1 agacatttct atgattttaa tatagtaaaa agagccgtca cggaaaatcc gtcacggctc
       61 taaacaaaat atgagaggct gtttttgttt ggctattctt attacttgat gcagggcagc
      121 ttgtgtcaag acttctttac atcaaacttt ttaattttac attgggatat ttatccgcaa
      181 accagcgttc tgcaaattgg ttctcgaata agaacaacgg ttggtcgaaa cggtcacgag
      241 ccaagatatt gcgacttgaa ctcatcttct catccaaatc ttctggatcg atccagcgag
      301 cgattttgtg tcccattgga gacatgatta cttcggcgtt atactcattt aacatacgat
      361 attggaatac ttcgaattgt agctgaccaa cagctccaat gatatattct tccgttagat
      421 atgttttata aagctgaatc gctccttctt gaactaattg atagattcct ttgtggaagg
      481 atttttgttt catgacattt tttgcagtca ctttcataaa cagctctgga gtaaatgaag
      541 ggagttcttc ataagcaact tttagttttc cttcgaacaa tgtatcccca atctgataat
      601 tgcctgtatc atatacaccg atgatatcgc ctgctactgc tttttcgacg ttttctctcg
      661 aatctgccat aaattgtgtg acattgctta atttcagttt tttatttgta cgttccaacc
      721 agacatccat tcctcgttcg aatgttccag aacagattcg gacaaaagcg attcggtcac
      781 gatgggctgg attcatattt gcttggatct tgaatacgaa acctgaaaat tcttcctcat
      841 aaggacttac catctgatca tcttgtgttt tgtgtccata aggagctggt gccaattcaa
      901 cgaaagtctc taaaaaagtc tgtacaccga agttagtcaa tgcagaacca aaaaatactg
      961 gtgtctgatc gccacgagca attttttctt gattaaatgc atctccagcc tctttgatta
     1021 gttctacttc ttctaagact tgttcatata aggaattttc agttagagga tgattttcgg
     1081 gaagcagtcc atcttcatcc aattctacta atcgttcatc ctgataattt tccggacgat
     1141 aaaactctac acgattatgg tagatatcat aaagaccttc tagcccttta cccataccga
     1201 ttggccaatt catcggataa gattcgatat ctaatagctc ttctaattct tctaacaatt
     1261 ccaatggttc acgaccatca cgatctagtt tgttgataaa agtaaatatc gggatgccac
     1321 gtttttttac aacttggaat aatttctttg tctgtgcttc gatccctttt gcactatcga
     1381 ttaccatgac cgcactatcc acggccatca aagtacgata agtatcttca gaaaaatctt
     1441 catgtcctgg tgtatccaag atatttactc gtttcccttg ataatcaaac tgcataacag
     1501 aactagtaac agaaatccca cgttgtttct cgatttccat ccagtctgat ttggcaaaat
     1561 tacctgtttt tttgcctttt acggtcccgg cttgacggat agccccacca aacaatagta
     1621 attgttctgt aatcgttgtt ttcccagcat ccggatggga aatgatcgca aaagttctgc
     1681 gattgtccac ttgttgcttt aattttggat tattcataac ctaactccat ttcttccatt
     1741 caattattta tattttctct aggcacagat tgtccgatct cttaaagaaa gacaatttca
     1801 tatagggctg tgacaaaagc cgtgtagcag tctctttttc cgagtaaacg gcgttctaga
     1861 gacagcctat cagcaataag cccacccaag ccaaaaatac gaaaagctgt ttttgcttgg
     1921 ctgctcttat tacttaatgc aggatagctt tttcacaacc tttgtcaact actacaactg
     1981 aatcagtata gcatggattc agcgaatgaa aaagccacca aaccataaga gtcaaatatt
     2041 tttttgtcag ctgactaaaa gaaaagacgt gttgcttgtt tattcaagca acacgtcttt
     2101 cataaccaag tttccaatta tcttcttggc tcgtcatctt cgaattcttt tctgaagtat
     2161 cttcgttctt cttcaggttc cgcttctgtt tcttccggat catgaaaaat gacacggatt
     2221 ttaaggattc gagaaccttc catttcttca gaaaccagcg taatattatc gacatcaaac
     2281 gataactttt cgccttcatc cggaatcatg cctaatgctg taatcaaata accagccatc
     2341 gtatctacat cactcatatg cagatcactg tcaaaagctt cattgaactc atcaatcagc
     2401 atacgtcctt ggatgatata ttcgtgttca tcgatttttt cataaagatt ctcaacttca
     2461 tctgattcat catcgatttc tccgacgatt tcttctaaaa ggtcttctag tgttgctaac
     2521 ccaacgacgc cgccatattc atctaataaa atagccatct gattctgcgt ctttttcatt
     2581 tcatataaca gatcatcaat aaagaccgtt tcaggaacga ataaaggctc ctgcatgatt
     2641 ttcttgatat ctaaattatc aaagccgaat ttatgggcag cttttagtaa attctttgta
     2701 tgaaggattc cgactacttt atctttgtct tcgttataaa cagggattct tgaatagttt
     2761 tcggataaaa cttcattcac attttcttcc acagtgtcgt tgatatcaat cataaatgca
     2821 tccgttcgtg ggaccatgac ttctcgggca actttcgtat caagagaaaa aaccccttgg
     2881 agcatttcta attcttcatt attcaatacg ccttcgtttt caagcatata acgcatttca
     2941 tctctggtca tttttgaatc ttcatcatca aaagtcatcg gcgtgatacg cgccaataaa
     3001 cttgtggaag cagaaagcag ccagacgaag ggcttagcta ctacaccaat caagcgtatc
     3061 agccctgaag tgaactttgc gacttcttct gatttattta aagcaatacg tttagggtac
     3121 aattctccaa aaacaatcga tacataggtc aaaatagcca gcacaatgat atttgcaata
     3181 ttttttgccg ctgtgccgcc acctaatacc ggagctaacc gttccgataa agtattagct
     3241 agtgaagcac cagacaaaat gttgaccaaa gtgattccta cttggattgt cgataggaaa
     3301 ttatttggat tctgcaatat ttttaaaagc ttttgagatt tcacatctcc ttcatcagct
     3361 ttttgctcga ttcgattctt attgatcgaa acaacagcaa tttcggaagc tgctaaaaaa
     3421 gcattgatca atgtcaaaac gatcaataat aaaatctgcg ctaacagcga ctgactctca
     3481 gggtcagcat tcataatcgc attctctcct tataaaatta atttttagtt tacgatagaa
     3541 aaaaacgtgg cactatagcc acgttaaaat ggtatcacaa agcaattttc tttgcaataa
     3601 aaacaagtgt actaaaacta cctacgaggt cgtagcaatt ttcttcttgt tttcattttg
     3661 cttgtcttct ttgacgattt tgattatata ggaaatgatc gtcttgcaga ttgcgtaaaa
     3721 aggaacacct aagaagatac caagcaatcc ggcaatattt ccagctacta gaagaacgat
     3781 aatgatcgtt aatggatgga ttttcagtga tttcccaatt acattaggat aaatgatatt
     3841 cccatccaac tgttggacga ttagcacaac cacacaacaa agtaatgctt ttaccggatc
     3901 attgaagact gttactagaa atgcaggtgc tagtcctaga taaggtccta aatatggaat
     3961 taggtttgtc aatcccgcaa tgaccccaaa taagaaagca taatccacac caattaccat
     4021 atatcctaaa aatgtgaagg taccaacgaa taaacattcg attgcttgtc cgctaatgta
     4081 attagccaat gttttattca gttgccccaa taactccaca atttggtttc ttcttttttc
     4141 tgggaaaaac tgttggatgt ttggcactag tttttctccg tctttcaaca tatagaacaa
     4201 gatgaatggt gctgtaatga ttactacggt tgccgaagcg atcgttccaa cgatcgaacc
     4261 taaactattg gatactccac taaggaattg ctgaatgatt gtcccgtaag aaatgtctaa
     4321 ttgctcaaaa tatttattga tatctacttc cttgaacaaa ggcaattcgg ctacctcttt
     4381 taaccagctt tctacctgtt tgatgaaatc aggcatatta gatgccaatg aggaaatttg
     4441 ctgtactaga cttgggatga cactaagtaa aatccaaata attgctgcaa ctaaaaggag
     4501 cagcacaatt gcgacagccc atattctttt gatttttgct ttcatcaaca gcttgacaat
     4561 aggattcaat aaatagtaaa gaaagcctgc aatcagtaca ggtgcaaata acgtggaaaa
     4621 aaatgtcccg atcggttcga aaataaagtt tatttctgat gaaatcatga tcaatgcagc
     4681 tatgatcaat aattcaagcg accaaaacat tagtttagac tgtctaagtt tttcaaacaa
     4741 tagatttcct cctttctttc ttactagtat atattatatt cgttaaaaat tcttttattc
     4801 tttttttatt tttgaaaact tgccatactt tttatttgtt ttattttcca gtatactata
     4861 cgaaaggaag gtggctagac catgaaagtt gtacaaacaa aagatacaat gagcaatatt
     4921 tacttagatg ctgtacggat caggcatcaa gtttttgtag tcgaacaggg tgtgcctttg
     4981 tctcgagaaa tagacaaaga tgaagcccat tgtatccact tcgtcctgta ctcagataaa
     5041 aaagaaccgc aaggaactgt acgtcttttg cctttagaaa acggaaaaat gaaattacag
     5101 cggatggcga ttctttctga atacagacac caagggttag gaaaaatatt gattgaagaa
     5161 gcagaaaact ttgctaagaa tcaaggctat aatacgattt tattaggggc acagtccaca
     5221 gctgaaactt tttacgaaaa actaggatat acagcttacg gcgatccttt tgaagatgct
     5281 ggcatgcctc atatttatat gaaaaagact ttataaaaat ttatttcgaa aaaagcaaaa
     5341 aacagcaagc ttttgagaaa aatctctcac tggcttactg ttcttttttg tgtttactta
     5401 cgcgacaatt gcttgtaacg gttataccaa atatcaatat agccttccga gaacgggcca
     5461 tctccattat tgatccagtc aaccagaatc ttcacatttt cttttaggat gaagtcaata
     5521 tcgttcgggt aattcatcat tttgctgtgc atctcatatt catcgacatc caatagtcgt
     5581 ttctcgccgt caggaaatac cttgatatcc aagtcatagt cgatatattt caaggcctca
     5641 tcgtccagca aaaaaggtga agctagatta caataatagg aaaccccctt ttctctgatc
     5701 attgctatta cattgaacca gtatttttta tgaaaataaa caatcgctgg ttcgcgcgtt
     5761 acccatcggc gtccatcaga ttcagtgact aatgtgtgat cattcacgcc gatcatagag
     5821 tgttcgcttg tttttaatac catggtatct cgccacgttc gatgcaattt gccgtcgtgc
     5881 ttgtaacttt ggatcgtcac aaactctcct tcttttggaa ctcccatagc aacccttcct
     5941 taagcagcat tttgtgcttt gtgtttgggt gttcattaca ccgacatcat aattattata
     6001 tcaataaaaa tgagctttgc ctagaaagga ttcctattgt ttcgattttt ctctaactga
     6061 tctaccagtt tcatttggac cttaggaaaa acgaccgaat caaatgcatc aggcttcaac
     6121 cacttcgaag ttttgttctc ggtaaattct tgttctgctc cctcggttgc ccgtccgtaa
     6181 aaaaggagaa catgccattt cagatgacta aaaatatgag taacttctcc taaatgtcgt
     6241 gtctgccaaa caaagagttc ctgcttttca aatgggagat tttgctcttc ttctgctact
     6301 aggtcatcaa acagatcgat ttctggcttt gtttcccatt cttttttcaa tcgctcatac
     6361 tcctcttgtg tcacttccat cattggaaat gtccacatat tcgccagcaa tttttggcta
     6421 tctctttctt caaaataata cgctccagaa tgattttgaa gagctgcact gatataataa
     6481 acgtcttttg gctttgcttt ttttgttttt acaggaaaac tcgtttggat tcctcgtttg
     6541 ttagctaaac aaaaggcttg gatcggacaa gtctcacact ttggagaagt tggtgtacag
     6601 atagctgaac ctagatccat cattgcttgg ttgaattcac ctggatgctt ttcatcaatg
     6661 attttccgca tcgcttcatc aaagattttc ctgctagaag cttttgcgat gtctgcttca
     6721 atgcaaaaca atctgctgac tactcgcatc acgttgccgt caactgcagg ttctggaagt
     6781 ccaaatgcga tacttgcaat cgcgcctgtc gtatatggtc ctattccctt caatgagcta
     6841 atttcttcag gcgtttgcgg catttcccca tcaaattctg acatgatctg tttagcagca
     6901 gcttggatgt tccgggctct tgagtaataa ccaaggccct cccatgcttt taaaagcttt
     6961 tcttctggag catttgctag ctcttcaatt gtaggaaacc attccatgaa acgataaaaa
     7021 taatcgatca ctgtgtctac tctggtttgc tgaagcatga tctcagagat ccagatacga
     7081 taaggatcac gattatagcg ccaaggcagg tttctttttt cttgttcata ccattgaatg
     7141 aactgatctt gaaattcttt tgtttcttta tctgtccatt tttcccattc tttattcact
     7201 ttcctcttcc ttttccgtaa tgaatcgctg gatcatatcc aaatcaaatc ctttttgata
     7261 taagctttgc tttactttta gatttctttt tttaggttca aaccttaaat ttcttcgcca
     7321 tagtttatct ccttgtttga ctaaataatc atattctatt tcttgatctg cttcttgcgg
     7381 aagatcagct atcgcttgtt ggatcacttc ttgagaaaag cctttcgtca tcaatgtttg
     7441 ttggattttt ctcaataatt ctttttggct ttttccgtga ttttttccaa acgctttttc
     7501 ggctgtctga tgtgcatttt gaacttggtc ttcaaatgta tattggctga gcgcttgttc
     7561 aatgatttca ggtttcaaac ctttttgctg catctgctgc gctaatttct ttggtccttt
     7621 gtcacttaag cgcatattcg tgcgcacata actttcccca tagaccaaat catcgacaag
     7681 gtccaattct tttaagcggg ctacgatttt atttcgatct tcttgaagga tttccttttc
     7741 ttttaagtat gttcggattt ctttttccga tcgcaactga taacttaagt aattcattgc
     7801 taggtgaaaa ccgaaatcag ctccgctact cttcttgatc tcttggatag tttcttcgtc
     7861 taattcccgt ccttttaata atcgatagcg tactaataca tcttcagata cttgtaaagc
     7921 ttctccttca gataaatcta cgcgatagaa agggcctttg cctttactga ttctcgtaac
     7981 ggttaacata aacatcctcc tcgaacatat attctctatc ataccacaga aaatccccgt
     8041 aatcaggtat tatatgttag aataaaccac agaaaaaaga aaaagggaga tacttatggc
     8101 agaaacaatt gtcaaaactg gacaaacggt tcctttgaaa atcaagcgtt taggcataaa
     8161 tggtgaagga atcggttatt tcaaacggtt gatcatattt gttccatatg cactaccaaa
     8221 agaagaagta ctagtgaaaa tcacgaaagc aactcctcgc tatgcagaag gagaattaat
     8281 aaaagtcaaa aaaacaagca aagatcgcgt agtggcacca tgtcctgttt actatgaatg
     8341 cggtggctgt caattacaac acttagctta tcaagcgcaa ttggatttta aaaaggatct
     8401 gcttttgcaa gcattggaaa aattcaaacc agcaggcttt cgatcttatc aacttttgcc
     8461 gacgatcggg atggatgagc cttggcatta tagaaacaaa gcacaatttc agttacgaaa
     8521 aaataagcag actcaaaaaa ttgaagctgg tttatacgaa gcaaactctc atcagttagt
     8581 ccctataaca gactgcttgg ttcaagaacc tcgaacacaa aaagtaatga atactgttgt
     8641 tcaactattg aataaatttg atgcacccat ctatgatgaa cggacaaaca gtggaatctt
     8701 ccgcacaatc atggttagaa tcggtgttaa aactggggag ttacaagttg tttttattac
     8761 tcgcagtaaa aaattcccgc aaaaaaacgc aatgatccgt gagatcaatg aacgactgcc
     8821 cgaagttgtt tcaatcatgc aaaatgtcca ggcacaaaaa acgtctctcg tgatggggga
     8881 tgaaacgatc cacttatggg gaaaagaagc cattgaagaa caaatcaatg aagtgacttt
     8941 cgatctttct cctcgtgcct tctttcaact caatcctcag cagaccgaat tgctttatgg
     9001 cgaaggaatc aaagcattag atgtccagcc taatgaaacg atcgtggatg cttattgcgg
     9061 agttgggacg atcggcttaa gtgtagcaaa acaagccaaa gaagtacggg gaatggatac
     9121 cgttcctcaa gcaatcgctg atgcaaaaaa aaatgcggaa agattaggat atcacaacac
     9181 tcgatatgaa gtcgggacag cagagcaagt tctgcctaaa tggttgaatg aaggttttca
     9241 gcctgatggt atcatcgtag atccaccacg tacgggtttg gataacgcat tgatccaatc
     9301 attattgaaa taccctccaa aaaaacttgt ttatatctcc tgtaatgttt ctacacttgc
     9361 cagagatctg gttaatttaa gtaaaaagtt caagattgaa tatttacagt ccgtcgacat
     9421 gtttcctcag actgcacgtt gtgaagttgt cgtgaagatg acgagactcg ctgactaaat
     9481 gaaagaggtt gtgaaaaagt ctgccctgta tcaagtaata agaacagcca aacaaaaata
     9541 gctcttcatg ttttatgaat actgtacttg tgaccgaaga gttaacagga ggacactatt
     9601 tatttaaaaa aagggttgtg acatagtttt tgtcacagcc ctttttgatt attattggat
     9661 attcacgtct atcacttgat aaaatgcatt tgtcgtatcg ctgatattcc atactgctaa
     9721 aatcacatta tatccttttc gatcctgcgg aagtgtgatc gtttggttga ctgtttttgg
     9781 tggaacagtt gctttgtcgt ctatttttgt gatcaattca aaatcactaa atttcaaagg
     9841 ttgattagga ttccaagttg gtttggtaat gtagtaatcc caagtacttg tccgatgttg
     9901 tgctgttaaa ttccaagtaa tatttaatgt tcctggatta accatgtttt tatgccatct
     9961 gttagatgtc tgttcatcta aaggctcaaa gccgctgact cctgcactag caatttttcc
    10021 atcaatgaat gtatttttgg tagcttcgat actttgcggt tcccattgtg ctcttccgac
    10081 attttgattt aagtttcctg ctgctgatgt tcctaggtat gcacggcttc ctggagaagt
    10141 aatataacca tgcgcaaaac ctggaatgct aaaaccgaac aaccctaaac taactaaccc
    10201 aataaacaat aacgtactaa tctttttcat gtagaaacct ccttcatttg ataaccacat
    10261 tgtaaacgct ttattgtatt aacacaaaca ttatgctttt ataattttat gttgaagtaa
    10321 aaaaggtgaa aaaagtaaga tgcagattat aacagaagta gacgagaaaa gtacaaatag
    10381 aaaacatgag aatcaaatca tatgatcttt ttcaaaaaca tcattcatat aaacaaatgt
    10441 tcaacttttt aataacaaca attatcaatt tttttcttac ttcacaaact aaatacaggt
    10501 cttgctgtga gccttcatgg taaactacta ttatagaaag gagttagtaa aatgaagatg
    10561 ataaaagata ttcgtttttg ggtttgtgtt ataggaattg tcatattagg ctttttatca
    10621 gggttgctta gcggaaatcc cggagaatat tactattctt tgcaacttcc accatttgca
    10681 ccacctagct ggatatttgg gccgatgtgg accttactgt atatattgat gggtatttct
    10741 ctttatttgt tacttaatca caacaacaaa aaacagcgaa acaatcttgt cggtcttttt
    10801 gtgattcagt tcatttttaa ttttatatgg tctgctctat tttttaattt aagaaatatt
    10861 ttcattgcag caatcgatat tacactttta gttattttct tgtcagtgct tatgtatcag
    10921 ttgtggctac atcatcgcct ggcaatgtgg ctcatgattc cttattattt atgggtacta
    10981 ttcgcaactc tattgaatta ttcaatttat tttttaaact aagcgctaaa aagagacgtc
    11041 ctgtaattta acacatagaa aaagtgccac ctaagttaga ttttttgtct aatttaggtg
    11101 gcactcttta ttaaccaaac tatttttttc tagtacccac ttgttgttcc atactcatta
    11161 ttctggtcat aaccataacc attttgttcc tgtccagcgt aaggatcata actttgagta
    11221 tcttgacttg gtgtatagta ctgacttgta tcttctgttt gctgataata ggtattcgga
    11281 tcttggtact gatcatatgt gttttctgaa taggaagaat aatcggtgta cccttcacta
    11341 ttcgatccat caccgtttgt cgaatcgcca tcttccgaat catcaaagaa catcattgaa
    11401 gcattctcat ttttcaaatt aggtaatgta tcgcttgttt ttatatttaa ttgtttcttc
    11461 agttcatttt ggatcgaaag aagatcattt tttcctaaaa tttgatagta gatatcattg
    11521 atcatttgtc cttcgccttg caactggtct tgtttgattg tttcaaacgc aggggtgtag
    11581 ttcgtcgcta catcaagcat gtcatcccaa tctaagtccg tcgtcacatt gttctcaact
    11641 gcttttaaaa tttttctata attgcttacg ccatctaaac ttaaaacttt attgacgatt
    11701 ttcgtcacta cttcccgctg acgacgttgt cgaccgatat cgccttctgg atcttcgtgt
    11761 cgcattcgag aataggctaa acctttttct ccattcaatg tttgttcacc tactggtaca
    11821 tggacaccat ccaaagtaaa gtcaatttta ttattcactt tgatgccacc gaccgcattg
    11881 atcaaatctt ccatgccatc caaattgatc gttacataat gatcaatagg aatatcgagc
    11941 agttgctcaa ttgaatccat ggccatttcg acaccaccaa atgcataggc atgatttaat
    12001 ttatctaccg ttccataacc tacgatattc gtataaatat ctcggtccaa actgatgatc
    12061 gttgattttt tttgtttcgg attaactgtt actaccatca tcgtatcaga acgtccttgt
    12121 tccgttcttc ctaatccacc ggtatctaaa cctaatagca aaaccgaaaa aggttctttg
    12181 tcatcaatgc ttactctctc agaacgtttg gaggatattc gattagagtt tttagaaatt
    12241 ttgtttactg tttggctcgc ttctcccata aatctgattc cgtatgctga taatccggca
    12301 atcagcaaaa caaatacccc taacaaggtt aatagaactt tttgccatcg cttcataata
    12361 cacctctgtt tcacattttc taaaatattg tctcatataa ctgttcaatc agcaaattca
    12421 ctttttaatc ataagaaaac tttaaaaaac atttttgtgt aacgattact attttttaaa
    12481 atttctcaaa attcttaact atcataccat accgtgaaaa agatcgaaag accgaataca
    12541 cgcagttcgc aatcgtttta tcttttttta cgtatttttt ccagaaaaag cgaacaacaa
    12601 ggtattttcc aaataatcgt gcagtaaaat gatcgcacat acctttccca tctttttaac
    12661 agtctttgcc taccgttcag attatcattc gatttcacga aaatttcttc ctatgactgg
    12721 tccttttgtt aaacccatct tctattcgtt ttatttccag ctcactgcat ggctcttaaa
    12781 atgactcgaa ctagtgattt cattaccatc atcttttaat ggatagatat gctcctcttc
    12841 aaagtaaaac gattttcttt taaagtaata gaattgacat ggtaatgagt atgtatacta
    12901 gtggtttcac aaatggtctt ttattcaaca tccaaaaact gaaatttctt taaaatccat
    12961 tactaacgaa gaaaatgttc cttgcttgat aattagttag gacatgtcaa gtgaacaaat
    13021 gcatttactt agccaaaaaa tctaaaaact tcataaatag acaaaaagac acgactctct
    13081 aagaagtcgt gtctttattc aacaaattat tataatttag tcacttcagc agcttgaggt
    13141 ccgcgtgcac cttcaacaat cgtgaattct acttcttgac cttcttctag gctcttgaag
    13201 ccgtctcctt gaattgctga gaaatgaaca aatacgtcat ctccgccgtc tactgtaata
    13261 aacccaaatc ctttttcatt gttgaaccat ttaaccgttc cgtgttccat aaatgtatag
    13321 cctccaaaaa agttcttgca agtaaccttg caagaacatt atacccaatt tagaaaacgc
    13381 tagcaagctt tcgctctttt attttccaaa ttatttttaa gactgatatg acggaggaaa
    13441 tggttatttt gctgcttcga aacgtttgtt tacttcatcc caattaataa cattccagaa
    13501 tgctgcaata taatctggtc ggacattttt atatttcaaa taataagcat gttcccaaac
    13561 gtccaaacct agaattggtg tctttccttc catcaatgga gaatcttgat ttgcagtaga
    13621 ggtaatagct aattttccat tttccattac aagccaagcc catccagaac caaatcgtcc
    13681 agcggctgct tttttgaatt cttctttaaa agaagaaaaa tcaccaaaag cttcattaat
    13741 cgcttctttt atttctcctg taggttcgcc accagcattt ggtgccatga tttcccagaa
    13801 aaatgaatgg ttagcatgtc cgccaccatt gttacgtaca gctgtcttga tatctgttgg
    13861 aatagcgtcc atatcagata ataattcttc tattgttttt tcgcctaatt ctgggtattt
    13921 ctcaattgct gcatttaaat tcgtcacata agtattgtga tgcttatcat gatgcagatg
    13981 catcgtttct tcgtcaatat aaggttccaa tgcgtcatat gcataaggta gatctggtaa
    14041 tgtgtaagtc ataaaaaaat tcctcctcaa tgttttcttt ataatgtcta cattacaaag
    14101 agtaacaaag agaaggaaat tcttcaattg ttatgcctag tcgtcttttt cactttgacc
    14161 tacttcttct tcaaaaacct cagtttcttt aaaaataaac aacttgctga aaatatagtt
    14221 cgctaggata acaataattt gagtgatgat tttcgccaca tactcattgc tgttcataac
    14281 attgatcaac aataccattg cccccatgtc caatccgtaa gaaaggatac ggtagaagaa
    14341 gaacttaccg cattcaataa gtgcatgtgt aaaattcggt gattttgaat ggaataccca
    14401 aagtttgttc gttacaaaag caaacaatac agaacaaaac cacgaaatag aattggataa
    14461 ggccatactt aaatcaaaat attggcgtgc gagagcaaaa ctaacaaaat ttacaatcgt
    14521 agccaaacca ccaaaaaaga gatagactag tatctcccaa tatccttttg cttctaaata
    14581 tccttttaac tgtcgatact gcttcatgag ccaacctacc ttattaaaaa ttcttattta
    14641 tcatactcta tatttcaata actttcatct ttatttaaaa aaattaattt tttttgatcc
    14701 tttgcctttg tctctgcttg gatcgtcaca tgtgtaatac catattttaa ttttaataaa
    14761 gaaattatac gaggatatat cttttcaatc tcgcttagta gctgatcttc aaaatcaata
    14821 tgtccgtcga aaataattat cttttcgcta tactgccaaa tatggacatg atgaacattc
    14881 tttaccccat cgattgcttc aatttcttgt tccatttttt catagtcaat agcaggtgca
    14941 ctttgtatca aaatagcggc agcttttttg atcaccatta tcgcttctat caatatatat
    15001 atcgaaatga tcagtgtaat aactggatcg atccagtaga tatcgaaaaa acggatcaaa
    15061 attgctccga taatcacact taccgaagac agtgtatcac ttaacaaatg caaataacta
    15121 gattttatat tcaaactttc atctgcttct tgtcgtaata ataatactga gaacaaattc
    15181 gcgagcaatc caataactgc tactgttagc atcaaatgac tatttatctt ttctggtgaa
    15241 gtgaaccgct tgaatgcttc aacggttaaa aacaaggata atatgatcaa tatgaatgag
    15301 ttgatgaaag cactcaatat ttctaaacgc tggtagccat acgtacgttt tttatttgct
    15361 tcttttcggg ccattttttg tgccccgtaa gaaaaaataa ttgccaatgt atcactaagg
    15421 ttatggaaag catctgaaac aagagacaaa ctccctgaca taatccctcc aataaattct
    15481 gataaagtga tcaccgcatt caaaaccact actccaaata gtttttttga tgatctctgt
    15541 ggctgtgtag gcactattcc ttcctccatt ttatttatat tggatagtct ttagacaaga
    15601 ataacatttt tttgatattt caccaatcat tcagatgttt ccctaaagca cttgtatttc
    15661 acttgatact tcgctagaat tgcaagtgat gacactacta gatgaggagt acttatgaca
    15721 acaaaacaat taattatcag ttcctttata cgattcaaag acctaaagga gacgagaaat
    15781 gtcccatttt ggaaagccat tgtctatctt ttagttttga gtataattat ggctttgcca
    15841 atctcttttc aagtttttca agtcttaaac aacattcaat tggatgggca aaagattgct
    15901 actcgcattc ctgattttac aatccaaaac gggcagcttc aaaccgaaga aaaagaagga
    15961 ttcatttacc aaacaaactc cattatcttc acatttgatc cagagggaaa acgaacagaa
    16021 caggacatct ctactgattt gatggggaat tttgttagtg tcgggatgtt aaaaaacaaa
    16081 cttatcatag ctcttcctaa tacaggcacg acttctgctt tgttgaacaa taaccaatta
    16141 gaacttccct acaccaatga atctttaaaa aacttaacag gtaaggagct ccgttctttt
    16201 ttaagtaaag catctatacc actttggata aaagtcctga catttatatt ttctatttat
    16261 cctagttttc tgaatctgct gatcactttg ctatttacca acattgctgc ttttctctat
    16321 gcacgctttc gactaacaaa agctactttt cttgattgtt taaaaacatt gatctattca
    16381 atttcattgc ctactataat tgcaacaatc ttgatggtct ttcttccttc ttttgacaca
    16441 agcgccttca ttgcttttgc aggaatcttt atttttgctc aagctgtcaa aggatgggca
    16501 aaaatatcga tcaattaaga aataaagaca actcgttatt tctacttcaa tagacccttt
    16561 tagaggttgt gacatttttt tgtcacagcc ccttttttat ttgaaaaata tttctttctc
    16621 aaaaaacaag caaggcgctt caaagttctt cttgtatgcg ctacacttag agtaaagaaa
    16681 ggcgggaaat taactatgtc agaaagaatc atttatgcag atcgtccatt tcgtgatgaa
    16741 tttatcgaag aaatccatac tatagcgcct ggttatatct ttaaaacaga attacaacca
    16801 gaggatctac aaaatgtaga gatcagtttg ggctggaact cagactacca agaaaaacta
    16861 ttagcctctt ctaccttgaa atgggtccaa tctatctcag ctggtgttga tcagctaccc
    16921 cttgagactt tttcaaagaa acatatttta ctttcaaatg gtagtggcat ccacagtgaa
    16981 tcgattacgg agcatgtctt aggaatcatc cttgggtatt ctcgtggtct ttttcaagca
    17041 caaaaagcac agagtgacat gaaatggtta ggttcatcgg tccattacca atctatccaa
    17101 ggaaaaaaaa tactaatcat tggaactggc catattggaa aaatgattgc aaaaaaatgc
    17161 aaagcattag acatgaactg ctatggaatc aatacatccg gtcatccagc agaaggaatg
    17221 gaaaagactt tttctttaaa tcaactaaag gaagttcttc ctgaagtaga tttcgtagtc
    17281 aatatcttac ctctaacgga ggaaacaact gggctctttg atcagcatat gtttgaacac
    17341 tttgatccaa aatctgtatt catcaatgtt ggccgtggtg cttctgttaa gacacaagat
    17401 ctggtgcagg ctttaaataa ccagcaactt tcctttgcag cattagacgt ttttgaggaa
    17461 gagcctcttc cacaagatca tcctctttgg aaaatggaca atgtactgat tacttcccac
    17521 attgcaggtt tgacacctga ttttcaaaag aaattaatgg ctattttttt aaccaatctg
    17581 aaaagctact ttgccaccca tgagcttcaa accaatcaag taaaattgtc tgcaggatat
    17641 taatagctga aaacaatatt ttcaaaagaa caaagagaag gtgacaaaa
//
LOCUS       DACKAC010000056        17428 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.057, whole genome shotgun sequence.
ACCESSION   DACKAC010000056 DACKAC010000000
VERSION     DACKAC010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.057"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            90..308
                     /locus_tag="I0B46_002004"
     CDS             90..308
                     /locus_tag="I0B46_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084282.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(597..1109)
                     /locus_tag="I0B46_002005"
     CDS             complement(597..1109)
                     /locus_tag="I0B46_002005"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAR2084283.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(1123..3420)
                     /gene="recJ"
                     /locus_tag="I0B46_002006"
     CDS             complement(1123..3420)
                     /gene="recJ"
                     /locus_tag="I0B46_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAR2084284.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3562..3996)
                     /locus_tag="I0B46_002007"
     CDS             complement(3562..3996)
                     /locus_tag="I0B46_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAR2084285.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(4006..4794)
                     /locus_tag="I0B46_002008"
     CDS             complement(4006..4794)
                     /locus_tag="I0B46_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAR2084286.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4791..5732)
                     /locus_tag="I0B46_002009"
     CDS             complement(4791..5732)
                     /locus_tag="I0B46_002009"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAR2084287.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5909..6496)
                     /locus_tag="I0B46_002010"
     CDS             complement(5909..6496)
                     /locus_tag="I0B46_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAR2084288.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6717..8030)
                     /gene="obgE"
                     /locus_tag="I0B46_002011"
     CDS             complement(6717..8030)
                     /gene="obgE"
                     /locus_tag="I0B46_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAR2084289.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            8318..9328
                     /gene="gap"
                     /locus_tag="I0B46_002012"
     CDS             8318..9328
                     /gene="gap"
                     /locus_tag="I0B46_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAR2084290.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9413..10348)
                     /locus_tag="I0B46_002013"
     CDS             complement(9413..10348)
                     /locus_tag="I0B46_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAR2084291.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            10534..11493
                     /locus_tag="I0B46_002014"
     CDS             10534..11493
                     /locus_tag="I0B46_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HAR2084292.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMDFRK
                     VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(11579..12673)
                     /locus_tag="I0B46_002015"
     CDS             complement(11579..12673)
                     /locus_tag="I0B46_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAR2084293.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            12862..13623
                     /locus_tag="I0B46_002016"
     CDS             12862..13623
                     /locus_tag="I0B46_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAR2084294.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(13738..14178)
                     /gene="fabZ"
                     /locus_tag="I0B46_002017"
     CDS             complement(13738..14178)
                     /gene="fabZ"
                     /locus_tag="I0B46_002017"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAR2084295.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            14398..15153
                     /gene="fabI"
                     /locus_tag="I0B46_002018"
     CDS             14398..15153
                     /gene="fabI"
                     /locus_tag="I0B46_002018"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAR2084296.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(15263..16276)
                     /locus_tag="I0B46_002019"
     CDS             complement(15263..16276)
                     /locus_tag="I0B46_002019"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAR2084297.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            16441..17361
                     /locus_tag="I0B46_002020"
     CDS             16441..17361
                     /locus_tag="I0B46_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAR2084298.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         5081 a         3728 c         3059 g         5560 t
ORIGIN      
        1 tttatcactt ccctctttac aaaccgaaca aatattcgta tactaaatat acgaacaatt
       61 gttcgcataa attaaagagg tgagaattca tgaaatcaat gagacgtctt ggcttagtat
      121 taactgtttt gtttttaggt attattattg gtaatttcgg catacgtcca gctttacttg
      181 tgtttgttcc gttatttact ttgtggttca tgttgtggga tgaaaagaag taccgtcaat
      241 ctgagcggcg cagaattcat gaagatcatt tctatcatga gccctactac agatccaggc
      301 aatcataata aggattgact gctaattatt caaaagacaa gaagttaaac ttttacgggg
      361 gtgtgacaaa aatcggtcac atcctttttt tcgaataaac ggtgttctaa agccagtttt
      421 tcggtattaa gtcaaattgc ggaaaaagtg attttttaaa cttctactgg tctttgcttg
      481 tttccattta taaaaaaata gcgaccctca aaagtcagat agagaatctg acttttgagg
      541 gtcgctttaa aatcgcttct ttaagaataa catcaagaat agcttctttt ttctgcttaa
      601 tattccatta atgtaatgat gtcatatcct ttgattttgt cgcgtccatg gagatccatc
      661 aattcgatca aaaatgcgca gcctacgact acaccgccta attgttcgat cagttcgatc
      721 gtcgctttga ttgttcctcc agtagctagc aaatcgtcac aaatcagcac acgttgacct
      781 ggtttaatgg catccttatg caatgtcaat gtatctgttc catattctaa tccataagtc
      841 acttcgatgg tttctcgagg taacttccct tttttgcgga ctggggcaaa accgactcct
      901 aactcataag caacaggaca tcctacgatg aatccacggg cttctggccc aactaccata
      961 tcaattcgtt tttcttttgc ataatttacg atttgtttag tagcttcccg ataagcgtca
     1021 ccatctgcca tcagtgggga aatatctcga aacacaattc cttttgacgg atagtcggga
     1081 atactggcaa tataatcttt taaatccact ctttgtttcc tcctaggttg ttaaccattt
     1141 ctttatggta ggaatatcgc tcaaaagcaa aaattcctca actttgattt tattgagccg
     1201 tcgctggtat aatctgctct cagtcaaagg atgactttct gggctagcga ctttttgtaa
     1261 aacaccgttc tctattgtaa caaatttcaa ttcaaaaaac acctgtatca taaaaattaa
     1321 taatttttca gggatacgca aatattgagc tattgctttt attttatgac gtatatcaat
     1381 tcgttcttga gaatggatca atttaaacaa ttttgcgtac tgttctcttg tgcctacccc
     1441 attcaaatag gcctcatcag gtgaaatccc catcagataa acacgattga atgaataaga
     1501 ccgaaaaact tctttcagat ccgctaaatc atctggacaa tccataacaa ctagagagtt
     1561 tggttttctt ttttcaatta ttgcctttac agaatctagt ccttcgaaaa caatcgcagc
     1621 ttcctggata atctcaggca cgaatttcaa cgtcgaagaa tcaaatgcta aataaaggct
     1681 accttccgac gttcggctct gttcacggaa acgttttgcc cgccaatcaa atacttgcag
     1741 accacccaca gaaaaatcag ataccatcag ctgcggtttc ttacgcccgt tccattcatt
     1801 gatcgaaagc tttcctacca catctacctg attattcagc agttcttctt cttgagtacc
     1861 aaaaccaaaa gcgaccgcat caagctgact cgatgcatcg gaaaggacaa atttcagatg
     1921 ctgttggttt gctccgattc ttttgatatt ttctgcttgg acttgtcgaa ataaaaactg
     1981 aggcaaagga ttatccgtac caaatggcgc caatagtttc agctcatcaa ttcgctctac
     2041 tgttacttca tttggcagca atacttcatc aattctcaac tcaggccctc tcatcaagtc
     2101 aatttgatga tcgactatat attggttcat cttctcctgt agtatagtga cattttcact
     2161 tggcatcgtt aaaccgactg ctgcatgatg tcctccgaag taagtaaata aatctcgcat
     2221 ctggtcaagc atttcaaaaa gattcaaggc ttcaatacta cgtccagatc ctttagctga
     2281 tccatcttct tttaaagtaa ggacaagcgt tggtttaccc gtttcattca ttattttccc
     2341 agcaacaatg cccaaaacgc cttcatgcca tcctcggttt accaatagat ggatatgatt
     2401 ctccttattg accatcgcta atgcttcttg tgttatctgc tctacgattg cttttctttc
     2461 ttcattgatc tcattcaatt tttttgcttg aacggatgct tcctcttcat caaacgtcgc
     2521 aagtaacgag acggcaggac gtggatcgcc catccgaccg attgcattta gtctgggagc
     2581 aatcgaaaaa ccgatcgttg tttcgtctgc gtccctcatt tttacgccgc tttcttcaaa
     2641 tagcgcctga agtcctatcc gttctgaatg atgaatggca tcgattccta atgaaacaag
     2701 gattcgattc tcatcagtca atgaaactaa atcggcgatc gttcctatcg ctactaaatc
     2761 caaaaattct gcaggcggtt cttccaataa tgcagaagct actttgaatg ctactccgac
     2821 tcctgccaaa tctggaaatg gatagtgccc cgctggatga cgagggtgaa caatcgcata
     2881 agcgtctggt aaattctcag gtagttcgtg atgatctgta atgatcacgt ctacaccagc
     2941 ttgctgagcg tatgctaccg catcatttcc tgctaccccg ttatcaacag tgatgatcaa
     3001 ctggattcct tcttcgatct tttcttggta taccgattgg tttggtccat acccatgctc
     3061 aaatcgattt ggcaggaata cttctacttc tgcaccaagt aattccagtg tttctttcat
     3121 taccgttgca ctggtgatcc catctgcgtc ataatcaccg taaacgagga ttttttctcc
     3181 actgattaca gcttcttgga ttcggctgat tgctttatcc atatcgtgta tcagatacgg
     3241 atcatgtaat tcttgttcct ttggatgtaa aaatcggttg atatcctttt cctcatggta
     3301 acctctttgc cacagtaact taccgacaaa tggagataat ttcagttcac ggatcatttg
     3361 gagaaaggat tcagaaggct ctgtctcgct taacttccaa tcaaaatttg cttgtctcac
     3421 tttctcactc ctaacttagt tattatagca aaggaaatca gcaaaaaaaa gcagaaagaa
     3481 agaataaaaa cagatcagat ccgctagaca gatgttcagc aggacctgat cttttgtacg
     3541 accatttatg aaatatccct attagtcaaa ataattgaag ctttgtttgg gcgattgagt
     3601 tttttgttct tccggcgctt tcaattgact gatttgtgcg gttttcgctt gtaactccgc
     3661 ctgataagca gcttccaact ccgcacgttc gtttctaaac tcgcgttgct gttcttcttt
     3721 tgcttcttca attttctttt gaagttctgt ttgatattct gacatttcct gagtcagctg
     3781 tttgatttgc tttttttgct gccatagtgt tgccgaagca gtcaaaaata caatcagtgc
     3841 accaatcagc gcagaaccga tgatcactaa gatcaatggc gcagaaaagg aagtgaagcc
     3901 aaaacttaca ggaacatttt tattgttcaa tacagcaaaa agaacaataa tcaaaactaa
     3961 aacaaatcca agaatcacat tgctttgctt tttcaattcc ttcacctact ttttgttaaa
     4021 aattccgctg gctaaataat caccaatcgt tggaaacagc gtataaaatt ttgatgcaac
     4081 ttctaaaata aacggttgat tgatttcccg ttttggatgt ctcatattgc ggacgatcat
     4141 ttttgccaac ttggcaggtt ctaaaacgat ttgtccgact ttttccaaat agcttccgct
     4201 tggatcagcc ttatcgaaga aatttgtctc gattggacct ggattcactg tcgtaacagc
     4261 tacgccaagt ggtttcaatt ctaatcgcaa agcgttggag aagccaagga ctgcaaattt
     4321 cgtggcagaa taaaccgttg attttgcagt tgccattttt ccagccatag aggcgatatt
     4381 aatgatatga ccttgtcttc tttctgccat atcgatcgca aatttttgtg tcaaaaccat
     4441 cattcctaag atgttgactt caaacatatc gtatgctttt ccaaggtcga atgtaaggaa
     4501 gtcttcgaaa atcccaaaac ccgcattgtt taccagaata tcgacgcgcc cgacttcttc
     4561 catcaccttt tcatataatt cgtccacgct ttcaggattg ctgacgtcta ccttgaaagc
     4621 ataagcctct ttgccgctca agcgtctgca ctcgtctctg acaccttcta caaatgccat
     4681 tcttcgtgcg caacttataa cgattgctcc ttgctttgct gcttcataac agatttgtgc
     4741 gcccagaccg cctgaactac ctgttactaa aacaacttta tcttctaaat tcatgcctct
     4801 tcctcctcgc gaaatggaat ctcaatgcta tcaaaatctt tcataatcgt cgtgttttca
     4861 aacacttctt gtgcttcttt ttcaagctct aaagctgctt ttcctaaata tctcgcactg
     4921 atatgagtaa gtagcagccg ttttgcctgc gcttcttttg caacttcagc agcttgatga
     4981 gtcgtggaat ggaaatagtt gtgcgccatt cttgcttcat ctttattaaa tgtgctttca
     5041 tgtaccagaa tatcgctatt ttccgcaagc acaacggaat tatgtgtttt tctcgtatcg
     5101 cctaaaatag tcacgattcg tcctttttta tctggaccta cgaaatcttt tccattgatc
     5161 gtccgcccat cttctagttg gatcgtttca ccttgcttca gtttgccgta cagtggtcca
     5221 gctggaatac ctaattcttt tagacgatcc acctgcaatt ctcctttatg atcatgttca
     5281 accacacgat aaccaaaact gtcgatccca tgatttagtt tttttgtata aacagaaaat
     5341 tgctgatcag taaaaatcgg ggctgtttca tttagctcaa tgaatttcag cggataactt
     5401 aaacgcgttt gcgaaatacc taaagagact tttatgtatt cctctatccc ttttggcccg
     5461 taaatctcta atggcgtatc cccaccttga aatgaccggc tgctgatgag cccgggaagt
     5521 ccaaaaatat gatcgccgtg taaatgtgta atgaaaattt taccgatttt tctcggacga
     5581 atagtcgttc gcaaaatctg catttgcgtg ccttcgccac agtcgaacaa ccaaacttca
     5641 ttgcgctcgt ctaaaagttt caatgctata cttgtcacat ttctatgttt tgcaggaaca
     5701 cctgcacctg ttcctaaaaa ttgtaattcc ataatctacc tgactttctt taagttcctc
     5761 tttaatttta gggtaaaaag aagggataat caaccgtaag tcgagaatgg gttcaaatta
     5821 aaaataacgt tctaaagcga tagcgttttc tttgaaaatt actataaaaa cagcagcgaa
     5881 cccgtttgag atcctctgct gttttttatt aatttgaaga tccagaactt tcattgtcgt
     5941 tggaagtttg cggatgtttg tccttccaag tcttttgtgc agcttctttc gcatgatttt
     6001 cagcttcttt cgcatcatgc acatcttttt cgacatcttc ataatccaag cgagtaattt
     6061 ctccgcctaa ttccatcatt accaattgat tcagtgggcg attgtcctct tcatcagcaa
     6121 tcaagatcag tcctgtagct ccttcaccga ttttatttcc tacaaagtcg aacacggtct
     6181 gggcatcttt tatttctttg gcatcttgtg tcgcaccgat cattcctccg gcaaaccatc
     6241 ctaataaaat ccctagcgga ccacctaata caccaaccaa cattccgatt aatccacccg
     6301 tagaagattt gttattaccg gtaaagtcga taaaatcttc tattttgaat tgatgtgtcc
     6361 cattaggatc atggctgaca actgccatct gttctccttt aatcgaacga acagcatgta
     6421 actttttgat ttctgaaaat gcctgatatg cctggctttc tacttgaaaa tttaaaatga
     6481 ttactcgttt gctcattaaa aacgcccctt tcataccttc ttgttattta ttttaccttt
     6541 aattgaaaaa taagaaaaga aaagcactct ttttttccaa taaaataggg ctgtgacaaa
     6601 agtcgtatca cagctccttt ttccgaataa acggcgttct tgaagctgat cacttctgtc
     6661 acaacctccc ttaaatctga atctatattt tttggtattt gtttttattt gcttgcctat
     6721 tcgacaaatt caaattcaaa ctcaccgata cgtacaaggt cgccatcttt cgcaccgcgt
     6781 gcccgaagag cttcatccac acccatacca cgtaattgac gagcaaaacg catcactgtt
     6841 tcatcatgat cgaaattcgt catttggaat aatttttcga tcttctcacc agataagatc
     6901 cacgttgcat ctgaatcacg atcgatttgg aactcaggac cttctggatt gaatccatag
     6961 tgtacagttt cttcttccag ttcttcttcg tacaatggga attctggcgt tacttctaac
     7021 aaatctgctg tagcattcaa taacgcgtcc aatccttgtc gagtcactcc agaaattgga
     7081 aatactggga tgtcatcagc aaattcatct tctttttcct tgttcagctg ttctttgaat
     7141 ttcactagat tttccggtgc atccggcata tccattttgt ttgccacgat gatttgcgga
     7201 cgctctagca agcgtaagtt ataagtagat aattctttgt tgatggcaag atagtcttca
     7261 taaggatctc gtccttccat cccactcata tcgatcacat gtaaaattac tcgtgttctt
     7321 tcgatatgac gaaggaattg tgtacccagc ccaacacctt gcgaagctcc ttcaatcaag
     7381 cctggcaagt ctgctgcggc aaaacttctg ccatcacttg tagtaaccat tcctagatta
     7441 ggtacgagcg tcgtgaaatg ataagcgccg atttttggac gagcagatga aataacggat
     7501 agtaaagtcg atttcccgac agatggaaaa cctacaagtc caacgtctgc caatactttc
     7561 aattctagtt cgatttttcg ctcttgtcct ggttcaccgt tttcagcaat ttccggtgca
     7621 ggattccgag gagaggcaaa acggatattc cctcgtccac cgcgtccacc ttttgccaca
     7681 actagtgttt gaccattttc aattaaatct cctaaaagag caccagtctc cgcatctcgg
     7741 acagtggttc cttgtggtac tttaacatag gtatcttctg aaccacgacc gtgcatccct
     7801 ttactcatcc cattttcacc tggctgcgct ttgaaatggc gattgaaccg aaaatccatc
     7861 aatgtacgta acccttcatc gacgatcaag atgacatcgc cgccacgacc accgtctccg
     7921 ccagccggtc cgccgtcagg aacgtatttt tcgcgacgaa atgcaaccat tccgtctccg
     7981 cccttgccag ctttcacatc gatcgttact tgatctaaaa acatggacat aattaagtcc
     8041 tcctattatt catttattta tttgactgcc tgaatttcca caataaacaa aagtcttact
     8101 tattttatcg gtgaaatctg attttgtcta tcatttcagc aaaaagattg tttaccttct
     8161 tcttttttta tcatttggta aggtttccaa ccttttttct gctaccaagt ataatttttt
     8221 gacaaaatat tctttgcgta gcagaataac agtgtattaa ttgtattaat tgaggatgcc
     8281 gtttcacagc acccctaata aaggaggaac ttttgatatg acagtaaaag taggtattaa
     8341 cggatttggg cgtattggcc gtctggcttt ccgccgcatc aaagaagtct cagatgatat
     8401 tgaagtagta gcaatcaacg acttaacaag tccaactatg ttagcacaat tattgcaatt
     8461 tgactctact catggtactt atcctggcac agttactgca acagaagact caatcgtagt
     8521 ggatggcgaa gcaactcgtg tttatgcaga acccgacgca agcaaaatcc cttgggcaaa
     8581 agaaaacggt gtagatatcg tactagaatg tactggtttt tatacatctg aagaaaaagc
     8641 aaaagcgcat ttagatgctg gtgtcaaacg agtagttatt tctgccccag ctggtgctat
     8701 gaaaaccatc gtctataacg tcaatgatga tacattagat gaaaacgaca aaatcatctc
     8761 agcaggttca tgtacaacaa actgtttagc acctatggct tatttcttaa ataatgaatt
     8821 tgggatcgaa gttggtacaa tgacaacagt tcatgcctac acttccacac aaatgctctt
     8881 agatggacct gtaaaaggcg gtaatttgcg tgctgctcgt tcagctgctg acaatactat
     8941 cccacattct actggtgctg cgaaagcaat cggcttagtt atccctgagc tacaaggaaa
     9001 attacaagga cacgcacaac gtgttccagt cgttgatggt tcattaactg aattggtttc
     9061 agtattgaaa acaaaagtaa ctgctgatca agtaaacgaa gcaatcaaaa aacacacagt
     9121 tgacaatcct tcattcgggt atgatgaccg tcaaatcgtt tctagcgatg tcattggaac
     9181 aactcaaggt tcgatctttg acccaacgca aacagaagta acaactgctg gtgatttcca
     9241 acttgttaaa actgttgcat ggtatgacaa tgagtatgga tttacttgcc aaatgattcg
     9301 cttactagaa aaattcgcta acttgtaatt tgatagatca atcgataaaa gaagcctttg
     9361 actggatcca gtcaaaggct tctttctttt catcccctac ttttaaactc gttcaatgaa
     9421 catattggct taaaaattgt tggactactt gctgatattt ttcaggatct gtcttgtatg
     9481 cttccgcatg ctcggctcct tttacaacat acttttcttt tggtcccttc gttgcacgat
     9541 agaccttatc taacatttca aatggtacaa atgtatcttc gtctccatgt ataaagagca
     9601 ttgggcgctg gtttttcttc aattgttcca ctgcatttgc ttcaccgaaa aagtagccag
     9661 cacggacttt tgtcactaga ctcgttactt gcataagcgg aaatgctgga agactaaaca
     9721 tatcgtctag ctgataagca agctcatcat gaacggaaga ataaccgcaa tcttcaatga
     9781 ttgctttcac attatctggt agtttttctc cgctagtcat cataactgtt gctgctccca
     9841 tgctgacacc gtatagaacg atttcttgcg attttccatt gttttctagc actttgttta
     9901 tccactgtac atagtcttta cgctctggcc atccaaatcc aatgtaatcc ccttcgcttt
     9961 ttccatgtcc tcttgcatca ggaacaagga cattgtaacc catatcatga aacatctttg
    10021 cgtaattggt catcgtttca gcgtttccca tatagccatg agccacaaga gctgtttttt
    10081 cacttttcgt ttcagcagga agataaatcg ctgataattt cagaccgtct tttgaagtga
    10141 tggtccaatc gcttcggttt tcttttgaac taaaccattt atgtgcatcc gtttctttgg
    10201 atgtctgtgt tgtaccaggt gtatcttcgt ctaaaaagtc tttctcagaa ggcacaacag
    10261 cataattata aaaataattc cctgcaaaca ctaacgcaac aatcagagct aaagcgacgc
    10321 ctaaaaggat tttcatccaa agtttcataa atcatccatc tcatttctct tttttcatat
    10381 aaccttgaat tagtataatc aaagaatctc tactatgcaa tgttcatttt tataacccgc
    10441 gaattgtaaa attaagttag aaaaataaaa aggcatttat ggtacactca aattgtattt
    10501 ccgacgaaag aaaacaaagg agtgtaacca taaatgacct acaaacatct taccatagac
    10561 gaactgacaa tgatagaatc atattatctt caacataata agccggttga aatcgctaac
    10621 cgaatgggac gtgctataca aactatttat aatgtagtca ataagttcaa gcaaggcaag
    10681 actgctcttg attattggca ccagtataaa gaaaataaga aaaaatgtgg tagaaaagtc
    10741 attcaattac ctgctcatga agtagattac attaaagaga aagtcactct tggttggacg
    10801 cctgacgtca ttatcgggcg aaaagaaagg tctgtttcat gcggtatgag aacactttat
    10861 cgtttatttt ctaaaggaat atttgatatt gacacactac cgatgaaagg taaaagaaaa
    10921 cccaatggcc atcaggaaaa acggggaaaa caacagtatc agcgctcaat ccatgatcga
    10981 cctgataatt atcctgattt caattctgag tttggtcacc ttgaaggtga tacgatcgtt
    11041 ggcattcatc ataaaagtgc cgtcattact ttagttgaaa gattatctaa agtcattatc
    11101 acgattaaac ccaacggccg taaggcatta gatattgaaa ctgcccttaa tcaatggttt
    11161 tctcgcttcc ctaaaaactt ctttaaatct attacgtttg actgtggaaa agaattttct
    11221 aactggaaag ccattagtaa ccaacatgat attgatatat attttgcgga ccctggaaca
    11281 ccttctcaac gcccattaaa cgagaattct aacgggattc tgcgtcgtaa tggactgccg
    11341 aaatcaatgg attttagaaa agtgaatcag acatttattt ccagtgtcag caatcaacgt
    11401 aatcatattc caagaaaatc attgaattac agaacaccaa tcgaaatatt tttgagctat
    11461 gtacaagaag cattttattc tagcttaatt tgacaaatca taacctataa aaaaacaaaa
    11521 taaaaaagca gaaataactg gctatttcta gcggattatt tctgcaataa ctaattatct
    11581 attttttatt ttcaattaat ttaaagcttg accatggttt caaaaagtca aggaggaata
    11641 attcgtcatc ggagattctt cctacaacat tgacacgtcc gtcgtttttc atatccttca
    11701 aggcgatttg tgtttctcct ttgtattgac cgtacccttc attgtcaatc agtacatccc
    11761 catgatgcat atctcgcgta ttatgaggcg ggaaatcttt atctttgtag tatacacgag
    11821 tcatcgttga acgaaggata tattctgagc gatcccctcg ataactatga aggttatcaa
    11881 acaagcagat tcgttcgttt tctgtaatac cttcttccgt atctactttc aatgttggat
    11941 aatccgcgtt aaatgcttct gccatttctt tcaattcttc ttcacttgcg tacgcatttc
    12001 caatagaaat atcatcaatc aatcctgtaa ggattaaatg cttcacttgt gtcgcaattt
    12061 caagatctcg gtggtcttcc agcgtgcaca agccatcttg cgtaggccaa gggccaaact
    12121 cagcagattg cgaattgacg aatgccatcg tattcaaatt atattttctg aatttttctg
    12181 aacaaaaaac aaagtgttcg tatcctaaac cagagtatcg atgaggataa aaattatgag
    12241 aacctaataa attatccgtg tttggcgaat aactcatgat gttgtcaacg taattcgtgc
    12301 ccgaactcat attgatctcg attttgatac caaaaggatt gcgcgtcatt tttgcttctt
    12361 ctgctcctgt gaatccgata tctaagcgga ccccgtaggc acccatctta tggaaaaaag
    12421 ataagtcatc gtaagaaatc tccaactgtt caaataacgc tggattgata tcaaccatta
    12481 cttccatgcc taggctattc gcgtaatcaa ctacttcttt aaattctgaa agaacttttt
    12541 ctttatcatc attgatctgc agcaaactgg taaaaacccg tttataccca tacttatgag
    12601 ctaaatccaa gtacgctttg tccttttcaa atgttgaccg ttctggataa atcgagattc
    12661 ctaattttcc catattacca tttcctcctg catcttttcg tttttgtatc cacttacatt
    12721 atagcaaaca caaagtaaat ggtctacacc ttttcttttt cttttaaaat aaatgataaa
    12781 ttccaataat ttaagtaggt attttccctc tattttgtta tcattaaaga taacaaaata
    12841 gtaaattgga aaggaacagt tatgaagaaa aataaagtta ttttaagtat tagtttagca
    12901 gcagcattgc tgtttgctgc tggttgttct gcaaacaaca cgacttcttc tagttcgtct
    12961 acagaaagtt caaaaagtac atctactgcg gaaagcacga ctgccagtag cgtcgatttg
    13021 aacgcattgg aattaccaca attgacgaaa gatgtcgccg aaaatgaaga tctggtcgaa
    13081 atggtgacga ataaaggcac aatcaaaatc aaattatttc ccgaacacgc accaaaagcg
    13141 gttgaaaact ttatgacaca tgcaaaagac ggttactaca acggactgac tttccatcgc
    13201 gtcatcaaag atttcatgat ccaaggtggc gatcctaaag gcgatggcac tggaggggaa
    13261 agcatttggg gagaaggatt tgaaacagag atcaacaacc acctttataa tataagaggt
    13321 gcgttatcta tggctcgctc acaagatcct aacagtaacg gcagccagtt ctttatcgtt
    13381 caaaatacag atgatatgca tgatggtctt ttaaaagacg actatcctca agcaatcatc
    13441 gacgcttata aaaacggcgg ttatccttca ctagatggcc attatactgt cttcggccaa
    13501 gtgattgaag gaatggatgt cgtggatgct attgcaaata tcgaaacaga tagttcagac
    13561 aaaccaaaag aagaagtaaa aatcgataaa atcaatatac tgcaagaagc aaaacaaaaa
    13621 taaaaaaata acgtctgaat cctgcataga tgacaacgta tgctaggatt caggcgtctt
    13681 ctttttacat aaaaagcaag taagcttcct tttgttttgc tatatcaggt tttatagtca
    13741 agcattttgg ttgacgatga acgtgaactc gcactcacag acttttttat catctacata
    13801 tgctgcagat aacgctgtac cgactgatcc cttcattttg gtgatttcaa aatgacattt
    13861 cagcacatct cctggaacaa ctttttgacg gaatttggcc ttattgatcc caccgatata
    13921 agctcttttt cctagaaact cttctgattt taggataagt atcgaacctg cttgtgccag
    13981 tgtttctaga atcaacgcac ccggcatcac tggattgtca ggaaagtgac cttggaaaaa
    14041 atcttcattg attgttacat tttttgtcgc aacgatcttt tttcctggtt ctaaagtatc
    14101 tacataatca atataacaaa taggataacg attaggaatc aattccatta cttcagtagc
    14161 agataatacg taagacataa ttaaactcct tctttcaggc aattgccatt ttcattactt
    14221 tgactatcaa agtatcatat tcctaaatat ttagcaatct ttttttaatt taagaaaaaa
    14281 tagtttgaca atcaaaacat tttgactaat aattaagaaa tcaagaagtt attttagcta
    14341 ttattctcta tttttttacg actatcaaat ttttatcaga aacaaaggag agtcattatg
    14401 tcatttctag aaggaaaaaa aatcgtcatt atgggcgtcg ctaacaaaaa aagcattgct
    14461 tggggttgtg ctgaagcaat gttgaaacaa ggggcagaaa ttatctatac ttaccaaaat
    14521 gaacgaatga aaaaatcatt agtcaaattg gtcggcgaag aagcattcac tgtagaatgt
    14581 gatgtttcta gcgacgaaag catcgaaaaa gcaatgaatg ccatcggtga atatgctggg
    14641 gagatccacg gccttgtcca tgcagttgct tacgccaaca aagaagaatt gtccggaaat
    14701 gtgtctgata tttccagaga cggctatcaa ttagctcaag atatcagcag ctactcttta
    14761 attgctgtat caaaatacgc acaaccgttt cttgcaccaa acagcgggat cgttagcatg
    14821 acttatctag gttcagaacg cgcaatcccg aattataaca tgatgggtat tgcgaaagct
    14881 tctcttgaag ctgctatccg ttatttggca gctgagttct cgcctaaagg aattcgggtc
    14941 aatggaatct cagctggggc aatcaaaaca ttagctgtca caggtgttaa agactatcaa
    15001 aaactgatcc agttatctga atctcgtacg cctgatcagc aaggtgtgac gatcgaagaa
    15061 gtcggtaatg cttgtgcttt cttaatcagc ccactttcca gcggaatcat cggtgatatt
    15121 atctacgttg acaaaggtgt ccatctttca taaacttttg ttgcattctt taaagtaaaa
    15181 catataaaaa tcaggaaaaa actttttaaa gtctcttcct gattttttct ttttacgttt
    15241 ttttcaactc attactttga aattattcca cgacttcaat ccagccttct ggtgcttcta
    15301 tgtctccaaa ttgaatacct gtcaattcat catacaaccg ttttgttact ggtcctactt
    15361 ccgtttcact ataaaataca tgaagattgt tattgtgata aatcccaccg attggagaaa
    15421 taacagccgc tgtaccacat gcaccagctt ctgcaaattg atctagatca tcaatgaaaa
    15481 tatctccttc aattgcttgc agtcctagtc gttcttcagc taactgcaac aaggaatatt
    15541 tcgtgatact tggtaagata gagggcgatt gcggagtgat gaattttccg tcttttgtaa
    15601 ttccaaagaa atttgctgca cctacttctt cgattttcgt atgtgtggct ggatcaagat
    15661 aaacacaatc tgagtaatcc agttcttttg cttctgttcc tggaagtaaa ctcgctgcgt
    15721 aatttccgcc gacttttgct gcaccagtcc ccttaggagc agcacgatcg taatcagata
    15781 caataaaatt cgttggtttt agtccacctt tgaaatacgc tcctactggc gtacagaaga
    15841 cgacaaataa atattctgga gctggatgga caccaatgtt ttcaccgacc ccaatcagta
    15901 aagggcgcaa gtataatgta gcgcctgttc catagggcgg cacaaaatct tcatttgctt
    15961 ttaccacttg cttgacagca tcaacgaaca tcttttcagg gaccattggc atcagcaatc
    16021 gtctagcact ttgattcatc cgtcgactat tttgttcagg tcgaaagaga ttgaccttgc
    16081 cgtcttttcg acgataagct tttagacctt caaaacattg ctgaccataa tgaagcgctg
    16141 gagagccttc atgaatcgtc aggtaattat cttctgtcaa ttctccttct tcccattgtc
    16201 cgtctttcca tttggcaata aaacgccaag gagttttgat ataagaaaaa cctagattgt
    16261 tccaatcgat ttgcatatac ttaccacctt cgttattttc tccacatttt atcattatcc
    16321 atattatttg tcattttatt ttcagctttt ttccttcatt atagttattt ttgctatttc
    16381 cttttttgaa aacttcttgg tacaatacag aaagaatcaa attttagaaa agaggatttt
    16441 atgttttcat tattagcaac tgccggtacg acagactctt ctggcgagac attgacagat
    16501 caagctgtca gcaaagtaaa tgcgtttcag cggttttggg acgggattga ttgggacgcc
    16561 attttggctg cctttatcca aaaaagttta tatctgattt ttttgatcgt ccttttttgg
    16621 attcttcacc gtgtgggaaa atatctgatt gataagtctt ttaatcagta ttctaaaaaa
    16681 gccattctaa acgagagccg cttaaagact ttacatagtc ttttaaatac tatttttcat
    16741 tatacccttg gtttcttttt tatctatgca attttatctg ctatcggtgt gccgatcgga
    16801 tcacttttgg ctggtgccgg aattgcaggt gtggcaatcg gtcttggtgc ccaagggttc
    16861 atgagtgatg ttattaccgg attttttatt atcatggaac aacagatgga tgttggggac
    16921 tacatcaagc tagcaaactt aacgattgaa ggaaccgtcg tatctgttgg tatccgtacc
    16981 cttcagctga aagcaacgga tggtactgtc catttcatcc cgaatcgaaa catcaccacg
    17041 atcagcaatc tctctcgtgc aaatatgcaa gtacttttag atattcggat tgttcctgaa
    17101 gaaggatatg acaaaatcta tgagatcatt gatcgagtaa atcgtacatt agctgaaaaa
    17161 taccaagaag agctccaaac tgaaccttct atctttggat tagtggatct tggaaacagc
    17221 aattttgcga ttcgtacggt ctgctacgca ttgaacggaa aacaatatgc cttaaaagaa
    17281 gagttcttga gctcctatgt caaagaattg acagcttctg gtttcacgat tccgaacagt
    17341 ccaattgctg tcggaaaata atttggacaa acgatgcaac gaatctcact aaaaagagcg
    17401 tgggacaaaa gtaaagaaca cttttgtc
//
LOCUS       DACKAC010000057        17349 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.058, whole genome shotgun sequence.
ACCESSION   DACKAC010000057 DACKAC010000000
VERSION     DACKAC010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.058"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..2305
                     /locus_tag="I0B46_002021"
     CDS             <1..2305
                     /locus_tag="I0B46_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084299.1"
                     /translation="LKDLLNAAGVNALFQSLISKVLALFCVVTLIYLGLKFFMSKHPP
                     KVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAANKADKTGSPAFQIIQNNV
                     IDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIITPDQAAEMADNDKSGLSDL
                     QKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYRRYSSKPGIILAGESSLAV
                     AYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSAIEDISQSFLLLAFTILEL
                     RIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQSVTKIFGVDTSLKNGSNS
                     LLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGFGHRKDVTPDNGEEQSDDK
                     ATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGTAEDLTEKAKDTLEEKLDG
                     TRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQVNAAVKANTTTEKENQEGI
                     VIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPKTDPEQYRYKPNSDLENEK
                     RISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKRISDLPKRNQEVQTDEDSI
                     VLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDNDQSFVHQSPAGTVQMKPSR
                     LPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQNVHTGYKPANVAFTAPTKI
                     NVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            2355..2687
                     /locus_tag="I0B46_002022"
     CDS             2355..2687
                     /locus_tag="I0B46_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084300.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
     gene            2688..3281
                     /locus_tag="I0B46_002023"
     CDS             2688..3281
                     /locus_tag="I0B46_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084301.1"
                     /translation="MRVKGQNLVDKKSIFQFGKPEERSILDLFPYKSCTEEGVLVTKE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            3444..5138
                     /locus_tag="I0B46_002024"
     CDS             3444..5138
                     /locus_tag="I0B46_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAR2084302.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTETQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            5826..7703
                     /gene="ltrA"
                     /locus_tag="I0B46_002025"
     CDS             5826..7703
                     /gene="ltrA"
                     /locus_tag="I0B46_002025"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAR2084303.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            7834..8211
                     /locus_tag="I0B46_002026"
                     /pseudo
     CDS             7834..8211
                     /locus_tag="I0B46_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            8211..9416
                     /locus_tag="I0B46_002027"
     CDS             8211..9416
                     /locus_tag="I0B46_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAR2084304.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFTDDGTWIDP
                     LPFLNSSKK"
     gene            9432..10076
                     /locus_tag="I0B46_002028"
     CDS             9432..10076
                     /locus_tag="I0B46_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084305.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTFKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNSQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            10087..10893
                     /locus_tag="I0B46_002029"
     CDS             10087..10893
                     /locus_tag="I0B46_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084306.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLSHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDHVKDEDYEQHAINYKVKGYKKEQKAAQKQIESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQMKDQEEIIQKLQEKIDRMTKENI"
     gene            10916..11146
                     /locus_tag="I0B46_002030"
     CDS             10916..11146
                     /locus_tag="I0B46_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084307.1"
                     /translation="MKRANLVGKDTFAEQKVLIKAYLKVVKAKEICQEVNLSNTTISE
                     LKNGRKSLDTLKFRSFEALYIAAKKYFTAERK"
     gene            11164..11403
                     /locus_tag="I0B46_002031"
                     /pseudo
     CDS             11164..11403
                     /locus_tag="I0B46_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332286.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11410..12282
                     /locus_tag="I0B46_002032"
     CDS             11410..12282
                     /locus_tag="I0B46_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084308.1"
                     /translation="MKSSDWMEHTWLPLKRNSKRKEFWILQLWPLGKLCSYLARFILD
                     LIIYSIVVFGTAYYLLNDPNRIAASDIFTVLMTLIFVMLLSIFTQIVMQFTRQKLTAG
                     KLFKSLFDLIIVNAILATLIKQIEQCGRTTKGVKEFLMTFVHFVNGHMYLFEVGIILF
                     LVGYVSTKLIKRSIEKEIEIDLIPTLFGYEYYVDFGRYDELINTGIVHTSIYEATVKG
                     IRTTLKDGQESKTIDRRKEVYNFYEVEASLRYGIGKLSLDGRNIPQNKPYIGWKSDAL
                     EVSKKLSMEEDELQ"
     gene            12279..13676
                     /locus_tag="I0B46_002033"
     CDS             12279..13676
                     /locus_tag="I0B46_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084309.1"
                     /translation="MSSINLNHLFEDISNHFQQFSIEDNEELMGLFQIEKEYDYLTFI
                     CREGRIIDPKESKIISHLRRFKNYISKNKETISEIKFDGTTNLEYLVRVTYQRNRRLI
                     EDDQGVLICAGEEYDSLKFTQLKFESIGRSIYFETRPFYIRYNDSQHLFLQDEPNGFT
                     ILERRGKKQITLSYERPLTFMDGSCESIFQKINPFIKEMMKENDSLPFTWVEIIEAKN
                     KYELLCKKYPSKKFTKKVNKYPLRYTYALIKLRPRVTSKQFRKIEAAIEQKDNDLFPS
                     EIFMCARKNSRKFVKDLLVSYVQNCILSNFATIILSDLMDMSFYLNQKLEFNFKTERG
                     LLRQHNQIVEEYNLKQAKKLKTFKLKQHGKYKLLIRHLQSHPHFTLIKTNKELFIEGS
                     TMHHCVYSYLNKIQKGGSIIWKYERQKHRYTVEIEKRKYGNYEVVQCYGKYDSLPDKQ
                     ELNEIKKIVRTIPYK"
     gene            14333..14641
                     /locus_tag="I0B46_002034"
     CDS             14333..14641
                     /locus_tag="I0B46_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084310.1"
                     /translation="MEVRIRNVDEKIVREIDRRCDELSNRTGNKWSRNDYLKLLVEND
                     FDRPLMEYKQDKFDQLLNQLIDKFSFVQSHNTKVLEEYICQNNQIIELLIEQSRGGEV
                     "
     gene            14643..15065
                     /locus_tag="I0B46_002035"
     CDS             14643..15065
                     /locus_tag="I0B46_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084311.1"
                     /translation="MAKVIDKHIYFYETHIDVLDEIIATHAEVKNYSEAVRFLCMNYD
                     SEVEKKVNSNQAKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKTISESVTTKSEVFDPTRFLKR"
     gene            15085..16674
                     /locus_tag="I0B46_002036"
     CDS             15085..16674
                     /locus_tag="I0B46_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084312.1"
                     /translation="MSKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEKERRTPEE
                     EQELRLIKNALEDFYIPEGETFSESKTGKEFSETSNEAQSLMKHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKVLTHSEQKEYQRLNQAIKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQTFQRGQQKGSIIYQDVVSHDHSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKKNTRKPYTEIENGVSYIVPKG
                     KRRQATLDEMKTNYVHELERFSYEKNRMGMEKRNLLTRKSDLRNTLTKVVKEPVRYDR
                     HAMSLLTEVYKNLPKKRSEWHYGDENRTKLSPYTREKLDELTQYVLTKEPGYQEYIQI
                     SKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQDSQIKPLIERMDQ
                     MQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEEK
                     YHASKAYEEQQRRIQMEQERQ"
     gene            16686..17021
                     /locus_tag="I0B46_002037"
                     /pseudo
     CDS             16686..17021
                     /locus_tag="I0B46_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            17162..>17349
                     /locus_tag="I0B46_002038"
     CDS             17162..>17349
                     /locus_tag="I0B46_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084313.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRSFVVTDKAAKAYRSGDI
                     LALNLITKTFVDAAEDYP"
BASE COUNT         6499 a         2660 c         3534 g         4656 t
ORIGIN      
        1 tttaaaagac ttattgaacg ccgctggggt aaatgcacta tttcaaagtt tgattagtaa
       61 ggtattggct cttttttgtg tcgtcacatt aatttattta ggcttgaaat tttttatgag
      121 taaacaccca ccaaaagtca aaaatatctt ggttaattta tttatggcca tgatcttgat
      181 ttttggtggt agttctttaa tcgatgaagg gttaaatatc agtaaaagct tttacggaga
      241 cgtcactgca gcaaataaag cagataaaac gggatcacct gcttttcaaa ttattcaaaa
      301 taatgtgatc gatgtcagta cgttactagc aactgacccg aataaagtag aatccctacc
      361 aacagataaa agaaatgctt taacagcgaa aaattttaaa acagcgaata tcaatgggat
      421 tattactcct gatcaagccg ctgagatggc ggataacgat aaaagtggat tgtcagattt
      481 acaaaaggat cggttaaagg cattacaata ccgattggaa ctagatgata atgggaaaga
      541 aattcctgtt caaattagtg acgaaggctt agcaaaatat gtctatactt cagggtatcg
      601 tcgatacagt agtaaaccag gtattatctt agctggtgaa tcttctttgg cggtggccta
      661 tttatttatc ctatttacga tcgttacttg cattattgag ctggtattta aaaaattcta
      721 tttagtgatt gccgcgtcaa cggactttga gacaggccaa cggatgaaaa gtgcgataga
      781 agatatttct caatccttct tgttattggc gtttacgatt ttagaattac gaatctatac
      841 attaatgctt tcaggtatcg gtgatttgca tgcggcagga aagatcaatg gattcttgta
      901 tgttgttgct ttaattgtgc taactattgc gctattcaaa ggctctcaat cggttacgaa
      961 gattttcggt gttgatacga gtctgaaaaa cggcagtaat tctcttctta gcatgtttgc
     1021 cttaggaaat attgcgaaaa atgtgggtag cggagctaaa aaccttgcaa gtgctggtaa
     1081 gagtggttta agccatttaa atgatattcg taaaaatgga tttggccatc gaaaagatgt
     1141 tacccctgat aacggggaag aacaatcaga cgataaagct acaactgtac ctaatagtaa
     1201 gaaaacagtt tccgaaatgt tcgggaaagc gaaaaatggt gtttctaaag ccgcagaagg
     1261 tcttggttac gtcaatgaac gtgggttaaa aggaacagct gaagatctta cagaaaaagc
     1321 caaagacacc ttagaagaga aattagacgg cacgagagct aaagaattgg ctgaagctgt
     1381 gaagaatcca tccgaagcac tagaaaaaca aaaagaaaaa gcttcagaag tcaaagaagg
     1441 actaaaagat gcgtatggtg aaggacaagt taatgcagca gtaaaagcta atacaacaac
     1501 cgaaaaagaa aatcaagaag gtatagtgat tccttcagtc aaagatcacc aagaagcgtt
     1561 gcactctaat gaaaattcga ttgaagagaa aaattccata gaagaaagaa attcagaaga
     1621 aggaaaaaat ggacagttac caaaaactga tccagaacaa tatcgctata aacctaacag
     1681 tgaccttgaa aatgagaagc gaatttcaga tttatcaaaa gtaaatcaag aagacaaagc
     1741 agaaaatgag ggacagttac caaaaactga tccagaacaa tatcggtata gatctgttaa
     1801 ctccgaaaat gaaaaacgca tttcagattt accaaaacga aatcaagaag tacaaacgga
     1861 tgaggattcc atcgttctgc caaatgataa tggaatcgaa gttggaactg aaaaatcaac
     1921 gaaaattggt ttacctaagg cacaaaattt accaatgaaa gatagtcgtt tgaatcaatt
     1981 caaggataat gatcaaagtt ttgttcatca atcacctgca ggtactgttc aaatgaaacc
     2041 aagtcgtttg cctgaaaatg gaaatctgac aactgtttct tacaatttat ctgaaagtgg
     2101 tgagacaggg aatgcgtccg ttcaaaatgt tgtgacaaga gaagctgcac caaaacctat
     2161 tcaaaacgtt catacaggtt acaagccagc aaatgtggca tttactgcac caacaaaaat
     2221 aaatgtgaca agagaaacta aaaacaggaa caatgcattg aaagaacaac tattgaggga
     2281 tatcaaacag gatatcaaac cataaaaggc ctacaagtta ggccttttct ttatatgaaa
     2341 aggagaaatc aattatggat aaatatataa acatgaaaaa ccctccaaga tttagtgggg
     2401 aatggaagtt tttcgccttt ttctttttgg atctattaac gcttggtggt tgcttttttt
     2461 gggcgaaaat agtcaataac cttgtccatt taccactctt ttttgcactc ttgaattatg
     2521 gcttatggat tgcgatagct ctagtgattg tttggcgacc ctataataac ccaggtcaac
     2581 gacaaatttt tgcgattatc gagtctattt tttttacgga caagaaccat tatcatgcgt
     2641 tagatcctaa ccgaaattat catggtacgt ataaggagga tgaataaatg cgagtcaaag
     2701 gacaaaattt agttgataag aaatccattt ttcagtttgg aaaaccagaa gaacgatcta
     2761 tcttggattt atttccttat aaatcctgta cagaagaggg agtcttagtt actaaagaag
     2821 atcattttca acgttattat cgtgtagttt ctacagacgt ggaaggatta aatgaacaag
     2881 agaaactaga acgaatgaat cagttgacca ccgtcatgcg tacgtatgtg ccaacaatta
     2941 agttagttag tttgaccaca gagacggatt taacggaaca aatcgtgcaa aagcggcaat
     3001 tattagataa aaatcgtatg gaacagtcct taggaagaaa tctacaacga ttaaaaaaat
     3061 atgaaaagaa attagtcgaa gaaatccagg aattgaaacg agcagaacaa gaacgtcctg
     3121 acttaagctt ctttttcgtc attgaaggaa agacaatcaa ggatttacaa accagaaacc
     3181 gtcagttaat gcgatctagt ggcattttag ggttaaaacc actctacaaa aaagaattga
     3241 tcaagattct gtatcgaatg aacaatatga atgatgagta ggaatggaga agagagaatg
     3301 aaatatccgt tgatatggtt agaaaaagat gttccgaagg aagagaccac tagaaaattt
     3361 cctcacactt taagcctggg taagttggga aaagggcaat cttttaacca agtaaagaat
     3421 caaacaagaa gaggtgactg tacatgttag aagcgattaa aacagctttt tcaccacaaa
     3481 atgatcaaaa agtacttgat ccattaaagc aactaggaaa tcgggaaata gaaattttaa
     3541 aatccaaagg gtatgattta gattttttaa aacaagtgat gccacaaggt gggttaaatt
     3601 ttgatgaaga atatgtgacg tatggtaatg gttattcacg atgtgtgaca gtttatcact
     3661 acacaaaaaa tcctacgctt ttttggttgg ccaatctcat gaacaatcct catacagtgg
     3721 ccacactcga tgttagcaca gatgaaaagc aaaaagtgat tgatcaaatc aaccgcgcat
     3781 tagatgaatt aaatgatcga tcagataatg aacgaaaagc gactgaccga aataaaagtg
     3841 cggaagaata cctggaatta gcggaatatg cccgaaaatt gacttctaat ggggaaattt
     3901 ctaaacaagt caaagtgaga gtacatgttt acggaaacac gattcaagaa gtagatgaac
     3961 gagcgcagga actgatcgaa aatctcaaag ggatggatta tcgtgcggtg acttatctgc
     4021 ataaaaccaa acgagattgg caatcgatgt ttaccatgtt cgatgaacaa gctgaatggt
     4081 tcggaaatat caaagggcaa tcactaccat cgttaaacat tggcggaggt ttcccgttcc
     4141 accaccaaac gctaaaagat ccaggaggga cacaatatgg ggaaaccatg acaggcggcc
     4201 catttgtctg gaatgcgacg tataaaaata gtgtacggtt gtctactaat accttgcttc
     4261 taggaatgat gggagctggt aaatcgaccg ttcttaaaat gatcgcggaa gcccatttag
     4321 ctgcaggaga ctttatttgg ggctttgaaa agggaaaaga ctttattcct ttcttaaagg
     4381 aatataacgg tattatggtg cgtttagacg gatctgacgg aatgatcaat cctttagaga
     4441 tatttgccac acggacgtat gatgaagcat ccagtttata tgacgatgga tcagtcaaag
     4501 atttgaagat caatgaagca gcgtcctatc aaacacactt agacaaagtg gtctatcagg
     4561 tacagttagt gagtcctcag ctaaaaggaa cgatgaaagc agaatttaag acctacttaa
     4621 atcgttttta tgaagagtat ggaacagtac cacgcggctt tacttcctca aattcgcgtt
     4681 caaatacgga gacgcaagtc acaggtaaag atccagaagc ttatccgacg ttcaaagaat
     4741 ttttagactt tttaggacaa ctggaattac caggtgcttc ccaagagaaa aagaatcgaa
     4801 aagaagaaat ggaatccatc gtagaatcct tatgtgagac gtatggcatg atttttgatg
     4861 gtcatagtac gattcgacac cttgaccaac aacagttggt cttttttgac attgactcta
     4921 ttacaggctt agatcgaggg gtacaacaat gtcaaatgta catggctatg acgttgattt
     4981 ggaatcaggc attgattcaa ggaagaagac aacgacgttt aattaaagaa aaacaaatgg
     5041 aagaacaaga cttcaaacgc ttcttggcga tgattgatga gtgccataat ggtgcgacct
     5101 gttctgcagt gaaaagctgt gggttgcaag tagtctaaaa actattgtaa taactcttat
     5161 aacgagaagt caaatgagtg ggtaacgcca caaagggctt ccactgatac tccgacatgc
     5221 aacaagtagt ttatgcagac caacgttgtg tgaagctcgg tgaagtcggc tgaaggatac
     5281 cgtaaggtaa aagaaatagt gaataccacg aaagttcttt agaggtaaag ggtgtatccg
     5341 ataagtcggg tgtccaaaaa aacttaatat ggtgagtatg tacaaacgta ctgacgaact
     5401 agcgaatgta ctgtccgaaa ggcatagctc ctagaaatgg gagagacagt gagcaaaaaa
     5461 tcactcattg tgctaacttg tggttgagta aaagtcgttg ttatgaaaga ccatgctgcg
     5521 ttacaggcgt aggagaagtt agggggcaaa agctagcatc taagttaagg catgtggata
     5581 gatataaggg aacaggggaa gccatcaaca tggagagtgc atactcgatg aagtggtagt
     5641 aagaaatatg tgaatgacaa ataacttact ttagtgatgg tgaaagtaga gtcacgactg
     5701 atgaaggttc tgtaatggaa ctggaggaac aggctcgatt cgtctgtaaa agtcatgaaa
     5761 cccgttcaac catgggattc tcgtatgact aaaaaggtca cgagccaatt ggagcgtgat
     5821 aaaccatgac aaaagaaaca agtccaaaag ataggaagtt gcggcatgca gaatattatg
     5881 gcatgacgga aatcttcgat aagatatact ccaaaagttt gaagggagat aatttcaaga
     5941 atctaatgca gattatcatt tctgataaca acattgtcct agcatatcgt aatatcaaga
     6001 gaaatcacgg tagtgtgaca agaggagaag atggagtaac gattaaagat attgaaaatc
     6061 tcaaccgtaa tgaattcgta gaaattgtta ggaagcgatt cgcacattat aaccctcgga
     6121 aagtaaggcg tgtagaaatt cctaaatata atggtaaatt aagaccgttg ggtattccat
     6181 caatatggga tagaatcgcg cagcaatgca tcttacaagt attagaacca atatgtgaag
     6241 caaaattcaa taatcacagc tatggtttcc gcccaaatag atcagcagaa cacgcaatag
     6301 cagattgtat ggcccgaatg aatcaaagtc acatggaata tgtggtagat gtagatatca
     6361 aaggtttctt cgacgaagtc aatcatacta aactcatgag acaaatctgg tctatgggta
     6421 tacgagacaa acagcttctc ttaatcatta ggaaaatgtt aagagcaccc gtagtacttc
     6481 caaatgggaa gttgatatac ccaacaaaag gaacaccaca aggcggaata cttagcccat
     6541 tacttgcgaa catcaatctc aacgaatttg actggtggat agcaaatcag tgggaggaaa
     6601 aagatgccaa agagctaaaa catcatttta ggaaaaatgg agcttgggat aaaggtaata
     6661 tctatcacaa gctcagaaag agtacaactc taaaagaaat atacctagtg agatacgcag
     6721 atgattttaa aattttctgt agaaattata atgaagcgaa aaaagttttc tatgcaacta
     6781 aaatgtggtt acaggagcgt ctaaagttac caatatctga agaaaagtca aaaatcacaa
     6841 atctttcaaa agaagaaagt gaatttttag gctttacgat gaaattagtg accaaaggta
     6901 acaaaaaggt tgttaatacg catataggta caaaagcaca aagcatgatt aaagtgaaac
     6961 taaaaaaaca aatcaaacga attcaaaaaa gtcctaatag caaagatacc tttatctata
     7021 ttggagtcta taacagtatg gttattggga ttcataatta cttcaaaatt gcgactcatt
     7081 gtagtgtagc aatgcgaaaa attgcaagag aactcagacc aagttttgaa atacgactac
     7141 gagaacaagg aatctcaaaa gaaggacaat acggaaagta cgaaggatac aagccttacc
     7201 tacggtcagg acaaatacga tatataagag ggtttccaat attaccagta gggtatattc
     7261 aacacagaca tccgatgtgt aaaaaaagaa atattaataa atacaccgaa gaaggtaggg
     7321 aagccattca taagaagcaa caaaccgtag aagaatggaa gatatcgtgg ttgtgtagca
     7381 atccagtgct tagtaagaga gcaacgattg aatttgcgga caatcgaatt tcaaaattca
     7441 tcgctcaaaa gggaagatgt gcagtaacag gtgaggagct tatcttatct gaaatgcatt
     7501 gtcatcatat tatcccttat catgaaagca aaagtgatag ctatgaaaac ctagtgattg
     7561 ttactgagga ggttcatcgg gtaatacacg ctacacaaag tgaaacgatt gaggaacttc
     7621 taaaatatct aaaattaaat ccaaaacaaa aagaaaaact caatgagttg agattaaaag
     7681 ttggtaacga agaaatatct taataaatat ttgtaggacg ggaaaacaga atgtctttta
     7741 tagacgaggg aacgccgtat gcggtgaaag tcgcacgtac ggtgtggact gggggaaaag
     7801 acggagataa tatcaaactc ttacctatca gtatcgatta atccagattt tattgaaacg
     7861 gtaaattatg tcacaaactt tcaacgagaa atgcgtaaag tagaagcaat gactgtttta
     7921 gctacacaat ctccacaaga aatggtgcca gaaaatatgt cttctgataa cttctcgaaa
     7981 ctgaagaaag tctttgaatt ttcttctacg aagatcttta tgcgtatgga tgaatctatt
     8041 ttacgtcata tagaaggatt agtgggtaaa tcaatgacga actcagaatt agaatccatt
     8101 cctcaacaaa atcaaggaga cgctgtgatc aattttggct caaaagaaac cattcgtgtt
     8161 cattttagtc cgaacaaacg tcaattaaaa ttgtttgatg gaggaaaata atggaaaacc
     8221 aaaatgaaag tttaatcaag cagtatgtta agcgtcgtgc aaagcggcgc ttatttttgt
     8281 ggttgtttgg tacttctgca ggtttgatta ctattttgat cactgtgttc gtgaccctat
     8341 ttctaatact tgcggcgggc tcgattgata attcggattc agattcaagc agtggtggcg
     8401 aagcttttac aggagaatat tcggaaggct taccgattta taaagaaatc aaaggaagag
     8461 gccctttttc tgatgagatt gcgcaatatg ccgtaggtgc agcagtgaaa tataaattat
     8521 taccaagtgt gatcctttcg caatacggtt acgaatcagc gtttggtaca tcggcgtccg
     8581 caagaaatga tctcaactat tttgggatta cttggtttga tggttgtctg tttcctaaag
     8641 gaactgcacg aggaattggt ggcattgaag gtggctggta catgaagttt cccaactcaa
     8701 aagctgcatt ttcctattac ggctttatgg tagccacaca atcaaatttt aatgcttgtg
     8761 tcggaaataa aagccctggc gcaagcttat tgatcttagg acgaggcggt tatgccgctg
     8821 caggaattac ggaagatagt ccgtattata caggttgtat gtcaattatc acttctaata
     8881 aattaaccga atatgatgaa ttcgccatca aacattgggg tgaaggtggc aataacaacg
     8941 gcacgattac tggtgaatgg acgaatccat tccctggcag cagtttagat aaaagttcct
     9001 tcagtggtgg tcaattattt ggaacaaatc caggcggtga attccgacca aatggtttcc
     9061 atgacggatt agattttggt tcagtcgatc atccaggaag tgaaattcat gcggttcatg
     9121 gtggaaaagt cgtgtacgtt ggcaatcctg gtatttcagg tttaggtgca tgtgtgatcg
     9181 tgattaatta cgacggtttg aatatggtgt atcaagaatt tgcgaatagt acaggaaatt
     9241 ctcgtgtgaa ggtaggcgac caagtaaaag ttggtcaagt aatcgggatc cgagatacag
     9301 cacacttgca tttaggtttc actcgaatgg attggcgtca agcacaagga cacgcattca
     9361 cagatgatgg tacatggatc gatccattac catttttgaa tagcagcaaa aaatagatca
     9421 ggagaaaaaa tatggaaaat aaaactttaa aaatcgtctt aacagctgca attagcgtta
     9481 gtttaatcgg caatatcgcg ttgttttggc agcttaaaga agaacaagag acaaatacct
     9541 ttaaaattga agaagtggct aaaaacgcgt ctgagaagct caaaaaaaat tcctctgagg
     9601 cacaggcgaa gatcgacaag ttaaatcaaa ccatcaatga gaaaaaccaa gaaatcgatc
     9661 agctgaagca acaagatggc acaaaagcaa aagaggaagt cactgaagca caaagaaaga
     9721 cggcagaaga gttcggtcaa ttggccattg ataaatcatt aagcagaaat gaattaacgg
     9781 aaaaactaaa agatgtcgca acccaagaag tgatcgacaa attaagtcct gcagacgatg
     9841 atcaccaaca tgatgattac ggaagttact cctttacatt aggtgacgtg caaacttacg
     9901 ctcaaacaag cagtgtaaaa gaggaacaaa actttgtggt atttgttgat tatacaattg
     9961 gcaattctca attcaaagat gtcaaaccac aaaaaataaa aggtggatta actgtcactg
    10021 aaaaacaggt agatggtcaa tggaaagtca cagactttag ttatttcact cgttaggagg
    10081 aagaaaatga gtaagaaaaa aggagcaaga caacgtttta aaaaacgaaa aagttatctt
    10141 agatggctgg gaatctttat gattctcggc tttttgattc ttgctagtcc gatttacacg
    10201 cataagaagg tagacatacc agatgaagta agcacgaacg cctttttaaa cacgaaggaa
    10261 ctgacgatgg tttcaaaaga aaaaggagcg aaccagcaat tagtttttga atttgcgatc
    10321 gacgatgaag atcagagctt attaaaagaa ctagctaatc ttaattatcg tgtagaagta
    10381 aagacagcta aaggggatta tcaagcaata aaagccaaag tgatcaaggt gtctgatgat
    10441 tattttgtag tgaagctatc gcatgtacca gaagaatata ttgctatgcg attaaccatt
    10501 atccctgaaa aaattgatcc aaaggtggat atgcaagaac cgcaagatct gatttattac
    10561 gttcacgaag ataaggtcaa agatcacgtg aaagacgaag attatgaaca acatgcgatc
    10621 aattacaaag tgaaaggata caagaaagaa caaaaagcag cacaaaaaca aattgaatct
    10681 ttccaagcaa ccatagattt gaatgaagaa ttagtgaaga aactaaaaga tcagttgcct
    10741 tatcaagttg cagacgatca ggaaaatact gaaaataaga ttaatagtta ccagcaagaa
    10801 attgaaacat cgaaaacgca aatgaaagat caagaagaga tcattcaaaa acttcaagaa
    10861 aaaattgatc gtatgacaaa agaaaatatt tagaagaata ggtggagtaa aagaaatgaa
    10921 gcgagcaaac ttggtcggca aagatacttt cgctgagcag aaagtattga ttaaagccta
    10981 tctcaaagtt gtaaaagcta aagaaatatg tcaagaagtg aatttatcta acacaacaat
    11041 atctgagttg aagaatggta gaaagagttt agatacctta aaattcagat catttgaagc
    11101 tctatatata gctgcaaaaa aatattttac ggctgaaaga aaataaaaac aggaggattt
    11161 tatgtgaaaa aagtatatca aaaatgttta tggatcagct atatcaccgt aggtatagct
    11221 gtttttttct ttagtttagc cgttatcgtc ggtacaattt ttctttggtt tatcaataaa
    11281 gatttatcat ttaattggtt catgagtcta ttgtcgttca ttatgttgat ctttttatgt
    11341 ttagaaatcc gcgaattcat gccttctcat tggcaagata agaggaaaga gtttagagaa
    11401 gaaatcgaaa tgaaatcgag cgactggatg gaacatactt ggttaccatt gaaaagaaat
    11461 agcaagcgaa aagaattttg gattctacaa ttatggccat taggtaaact ttgtagctat
    11521 ttggctcgtt ttatacttga tttaatcatt tactcgattg tagtatttgg aacggcttat
    11581 tacttattaa atgatcccaa taggattgca gcatcagaca tttttacagt gctaatgacg
    11641 ttgatttttg tcatgctatt gtctattttc actcaaatag tcatgcaatt taccaggcaa
    11701 aaattaacag caggaaaact tttcaaaagt ctattcgatt taattattgt aaatgcaatt
    11761 ctagcaactt taataaaaca aatcgaacaa tgtggaagaa caacaaaagg agtgaaggaa
    11821 tttttaatga cgtttgttca ctttgtaaat gggcacatgt atttgtttga agtgggtatt
    11881 attctctttt tagtcgggta tgtatccacg aaacttatta aacgttcaat tgaaaaagaa
    11941 attgagattg acctgattcc gacacttttt ggttatgagt actatgttga ttttggaaga
    12001 tatgacgaat tgatcaatac aggtattgtt cacacgagta tctatgaagc gaccgttaag
    12061 ggcatcagaa ctacgttaaa agatggccag gagtcgaaaa caattgaccg aagaaaagag
    12121 gtctacaatt tttatgaagt cgaagcaagt ctaagatatg gcattggaaa attgtcgtta
    12181 gatggaagaa atattccaca aaacaaacca tacattggct ggaaaagtga tgctctagaa
    12241 gtatctaaaa agctttctat ggaagaagat gagcttcaat gagttcgatc aatttgaacc
    12301 atttatttga agatatcagt aatcattttc aacaattttc aattgaagat aatgaggaat
    12361 tgatgggatt attccaaata gaaaaagaat acgactattt gacatttatt tgtcgtgaag
    12421 gaagaatcat tgatcccaaa gaatcaaaaa tcatttccca tttaagacga ttcaaaaatt
    12481 atatcagtaa aaataaagaa actatttcag aaataaaatt tgacggaaca acaaacttag
    12541 aatatttagt aagagtcacc tatcaaagaa accgccgact gattgaagat gatcagggag
    12601 ttttaatatg tgctggtgaa gaatacgata gtctcaaatt tactcaacta aaatttgaaa
    12661 gtattggtcg ttctatttat tttgagacac gaccatttta tattcgatat aatgattctc
    12721 aacatttgtt tttacaagat gaaccaaatg gattcaccat tctagaacga agagggaaga
    12781 aacaaattac attatcctac gagagacccc tcacattcat ggatggttca tgtgaatcta
    12841 tttttcaaaa aatcaatccg tttattaaag aaatgatgaa agaaaatgat tcattacctt
    12901 tcacttgggt tgagattata gaagcaaaaa ataaatacga attattgtgt aagaagtatc
    12961 cgtcaaaaaa atttaccaag aaggtaaata aatatccact acgttatact tatgcgttga
    13021 tcaagttaag accaagagta accagcaaac agtttagaaa aatagaagct gcgatagaac
    13081 aaaaagacaa tgatctgttt ccttcagaaa tatttatgtg tgcaaggaaa aacagtcgta
    13141 aattcgtaaa agacttatta gttagttatg ttcaaaactg tattctatca aattttgcaa
    13201 caattattct atctgattta atggacatga gtttttattt aaatcagaaa ctcgaattta
    13261 attttaaaac agaacgagga ttattaagac agcataatca aattgttgaa gaatataatt
    13321 tgaaacaagc taaaaaattg aaaacattca aattgaaaca acatggtaaa tataagttac
    13381 tcattagaca tttacaaagt catcctcact ttactttgat taaaacgaat aaggaacttt
    13441 ttatagaagg atcaactatg catcattgtg tgtatagtta cctaaataaa atacaaaaag
    13501 gtggttcaat catttggaag tacgaacgac agaagcatcg ttatacagtt gaaatagaga
    13561 aaagaaagta tggcaattat gaagtggtac agtgctatgg aaaatacgat tcattgccag
    13621 ataaacaaga actaaatgag atcaaaaaga ttgttagaac tattccgtac aaataagaat
    13681 ttatataaga catcagttta ttttgattga aaaaatgtag aaagttgtct gtgaaaaaag
    13741 tgtatgctat actgatgatg caaacacata aaacatttaa cactcaaaaa gagccctagc
    13801 agttgcagct gcgtaaggct cttttcttta ttcactatta accgaataat gaacaggcta
    13861 gctgtcttta tcatgtttag aatctaacca tttgttaatg agatgggaga aaacattaac
    13921 cacgattcta acacaaacat caagcacata aaacatttaa cactcctcct ttctggctga
    13981 agatttcaac caaacaggaa gacagcgtat caattatagc atacactttt tcttaaattc
    14041 atttttcata tgacaaaatg ttccttctaa tcgacaaagt tagaaggatt ttttattgtc
    14101 taaataaaca aataacctta atttttaaag agcataagga aacgtgggaa cactttttaa
    14161 aaaaagtgtt ccaataggct gtgcctatta cgtttctcaa aaataattgt gggaacaaaa
    14221 tatttaaaaa taggatagtg aaaggatagc aaaggatagt aaaaggatag aagcaggata
    14281 gcgaaaagga taggctcctt ttcgctatcc tttttaaatt ggaggaatta atatggaagt
    14341 aagaataaga aatgtagatg agaaaatcgt aagagaaatt gatcgaagat gtgacgaact
    14401 aagtaaccga acgggtaata aatggagtcg taacgattat ttaaagctac tcgttgaaaa
    14461 tgattttgat cgtcctttga tggaatataa acaagataaa tttgatcaat tattaaatca
    14521 gttaatagat aaattttctt ttgttcaaag tcataataca aaagtattag aagaatatat
    14581 ttgtcagaat aatcaaatta tcgaactttt aattgaacaa agtagaggag gagaagttta
    14641 acatggccaa agttatagac aagcatattt atttttatga aacacatata gatgttctag
    14701 atgaaattat tgcaactcat gcagaggtta aaaactatag tgaagccgtt cggtttctat
    14761 gtatgaatta tgattcagaa gtggagaaaa aagtaaattc caatcaagca aagttgaatg
    14821 caatgggaaa agaaatttct attttaaccg aaatggtttc agagtttgcg gatctccatt
    14881 tgaaagatac agggatttta attggtacaa attcaactgt ttaccaagaa gcaaaagagc
    14941 gtgtggaagg caagataaaa gcaaatcaaa cgaagaaata ttctcctaag ataaagaaaa
    15001 caataagcga atcagtgact acaaaaagtg aagtatttga tccaacaagg ttcttaaaac
    15061 gataaaaatt agaaggagaa tgtgatgagt aaatcacctg cagtcattct cacttgtcaa
    15121 tttaccttgc ccaataaaaa atctttttca acatacattg attatatgac aagagaaaaa
    15181 gcgctggaag aaaaggaacg acgtacgccc gaggaagaac aggaactgcg tctaatcaag
    15241 aatgctttag aggattttta tattcctgaa ggagaaactt tttcggagag taagacgggt
    15301 aaagaattca gcgaaacgtc gaatgaagcg caatcgttga tgaaacatgg aatcaatttt
    15361 gacaagttag aagaacaaga ttttaccaaa tatctttctt atatgacacg tcactatgcg
    15421 ttagaacaaa agaaagtact gacgcatagt gaacaaaaag aataccaacg cttgaaccaa
    15481 gcaataaaaa aatatcaaac agagccacac aaaaaagctt caggaaatct tcctggagtt
    15541 ttttctatgg cttcagacga agtgaaagga agtgatctga aggagatcaa gcaaaccttc
    15601 caaagaggac aacaaaaagg ctcaatcatt tatcaagatg ttgtctctca tgatcattcg
    15661 tatcttgaaa aaataggatt gtacgatccc aaaacagata ctttagatga agaaggttta
    15721 aaagcagctg gaagaaaaat gatggagaca ttgttcgaga aagagcaact gaatgaaaca
    15781 ggctactgga tggcaacgat tcaccgaaat accaaacata tccatattca ttttgcagtt
    15841 gttgaaaaaa aaaatacgcg taaaccatac actgagattg aaaatggtgt gagttatatc
    15901 gtaccgaaag ggaagagaag gcaagcaaca ttagacgaaa tgaagactaa ttatgtacat
    15961 gagttagaac gtttttctta tgaaaaaaat cgaatgggta tggagaaacg aaatttattg
    16021 acacggaaaa gtgatttgcg taacacctta accaaagtag tgaaagagcc agtacgttat
    16081 gatcgacacg caatgagctt attaactgaa gtatataaaa acttacctaa aaagcgttcg
    16141 gaatggcatt acggggatga aaatcgaacc aaactttcac catacacaag agagaaactc
    16201 gatgagttaa cccaatatgt gttaacaaaa gaacctggat atcaagaata tatacagatt
    16261 tcaaaagcat tgaaagaaga agcaaaaagt ctttatgggg aaagcaagag agaatcaaag
    16321 gatgcagaaa aaaatgctct ctttgattta aaaaagcgca caggaaatgc cattctaaca
    16381 gaactgaaga aacaggatag tcaaataaaa cctttaatag aaaggatgga tcagatgcaa
    16441 tctatttaca atgaacgatc ctttcgaaca cttaaacagt tgccaacgaa tgaatacaaa
    16501 tatagttacg aagaattcat gaagcgcagg aaacaattcc aacaaagagt tgtcacgcaa
    16561 cggagtttgc gtcaattaaa tcgaacgatt aatgcccatg aagaaaaata ccacgcaagt
    16621 aaagcatacg aagagcaaca acgaagaatt caaatggaac aagaaagaca gtaggaggaa
    16681 tagcaatgaa acaatattta gatcaatgga aagttataga aggatcatta agagaagaac
    16741 gaatcgaaca acttcccgat tgtttataaa aagagcattt gtttcagata agagaaatgt
    16801 tgagaaacga acagtttgat ccaaatcaat ttttagtcgt tgagtaccca gctactggtg
    16861 tgtactgttg taatcatgtg aagggtgaaa aatattttat cattcaaaaa tatgagggca
    16921 agttagcgcc ttattatact acttgggaaa tgaatgaaga ggggatcaat aacttcccgt
    16981 gtaaaagcat tgaagagtct atttcactca cagaatgtta gttgttaagt gaataaaaga
    17041 agtaaaagag tcctgaacat aatttgtcca ggactctttt tttcaatgaa ttgttttccg
    17101 ccaattcatt gagtggtgcg atttccgtct acattatatc aaaagaaagg aggaagaaga
    17161 aatggcttat tcaacgattg gagttgttgt tgaaaaaagc agagacaatc ttgtttttgt
    17221 gacagaaatt caaacaggta gatcttttgt cgtcacagac aaagcggcaa aagcttatcg
    17281 aagtggcgat attttagcgt taaatctgat tacaaaaacg ttcgtcgatg cggcagaaga
    17341 ttatccatt
//
LOCUS       DACKAC010000058        17232 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.059, whole genome shotgun sequence.
ACCESSION   DACKAC010000058 DACKAC010000000
VERSION     DACKAC010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.059"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            159..1418
                     /locus_tag="I0B46_002039"
     CDS             159..1418
                     /locus_tag="I0B46_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAR2084314.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            1424..2005
                     /locus_tag="I0B46_002040"
     CDS             1424..2005
                     /locus_tag="I0B46_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAR2084315.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(2087..2962)
                     /locus_tag="I0B46_002041"
     CDS             complement(2087..2962)
                     /locus_tag="I0B46_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAR2084316.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            3110..3442
                     /locus_tag="I0B46_002042"
     CDS             3110..3442
                     /locus_tag="I0B46_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAR2084317.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            3554..4762
                     /locus_tag="I0B46_002043"
     CDS             3554..4762
                     /locus_tag="I0B46_002043"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAR2084318.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            4916..5371
                     /locus_tag="I0B46_002044"
     CDS             4916..5371
                     /locus_tag="I0B46_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084319.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            5733..6008
                     /locus_tag="I0B46_002045"
     CDS             5733..6008
                     /locus_tag="I0B46_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084320.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            6021..8099
                     /locus_tag="I0B46_002046"
     CDS             6021..8099
                     /locus_tag="I0B46_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAR2084321.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            8262..10132
                     /locus_tag="I0B46_002047"
                     /pseudo
     CDS             8262..10132
                     /locus_tag="I0B46_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735758.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
     gene            10144..11091
                     /locus_tag="I0B46_002048"
     CDS             10144..11091
                     /locus_tag="I0B46_002048"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAR2084322.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            11111..11638
                     /locus_tag="I0B46_002049"
     CDS             11111..11638
                     /locus_tag="I0B46_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAR2084323.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(11701..12354)
                     /locus_tag="I0B46_002050"
     CDS             complement(11701..12354)
                     /locus_tag="I0B46_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAR2084324.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            12486..13328
                     /locus_tag="I0B46_002051"
     CDS             12486..13328
                     /locus_tag="I0B46_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAR2084325.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            13486..14382
                     /locus_tag="I0B46_002052"
     CDS             13486..14382
                     /locus_tag="I0B46_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAR2084326.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            14385..15098
                     /locus_tag="I0B46_002053"
     CDS             14385..15098
                     /locus_tag="I0B46_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAR2084327.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            15118..15636
                     /gene="msrA"
                     /locus_tag="I0B46_002054"
     CDS             15118..15636
                     /gene="msrA"
                     /locus_tag="I0B46_002054"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAR2084328.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            15633..15860
                     /locus_tag="I0B46_002055"
     CDS             15633..15860
                     /locus_tag="I0B46_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAR2084329.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            16012..16563
                     /gene="lepB"
                     /locus_tag="I0B46_002056"
     CDS             16012..16563
                     /gene="lepB"
                     /locus_tag="I0B46_002056"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAR2084330.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(16646..17188)
                     /locus_tag="I0B46_002057"
     CDS             complement(16646..17188)
                     /locus_tag="I0B46_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAR2084331.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
BASE COUNT         6018 a         2917 c         3543 g         4754 t
ORIGIN      
        1 tttggaaaaa gttctttttt tggcagaaaa aagcaaaaaa atacttgttt tagaaaaaat
       61 aggtataata gacggatacc aacatccaaa cgaaaaagaa ttattgagaa caatgacggt
      121 atttcaacaa atcgtcagtg agataagagg gaattgaaat gaatacaaat agcgaaaaaa
      181 tgttgcaagc tttatcagat gaagacttgt cacaagcaca aatcttttta gaaaaagcat
      241 taaaagaaga cccagcagat atattagctg aactaggcga agaattgctg gctattggct
      301 ttttgatcga agcaaaacag atttttgaac aactgatcaa ccaataccca gaaaatgacg
      361 gactgaacat cccgttagca gaaattgcaa tcgaagataa tgagatcgac ttagcttttg
      421 aatacctgga aaaagtggat aagcaaagtg attactatcc gcaaagctta ttggcgatag
      481 ctgatttgta ccaagttatc ggaatccctg aagtgagtga agcaaaattg aaagaagcag
      541 ctgggttgct tcctgatgaa ccattgatcc agtttgcgct agctgaattg tatttttcag
      601 ttgatcgttt caaagaggca gcagtggttt atgaaacatt gttggcaagc gggataaatg
      661 aaatttcagg aatctcgatg caggaacgtt taggacaatc cttaagtatg caaggagaat
      721 tcgaagcagc tatcgtacct ttagaagaag cactgaaaga agaaaggaca gatgatcgac
      781 tgttccagct agcttttacg aatcttcaat tgaaagaaaa tcagcaggca atcaactatc
      841 tggaagaact tcgagaagtt aatccgcatt atcaatcgtt atatctatat ctaggacaag
      901 ctcttcaaga agaagaaatg attgaagaag cacaaacagt gttagaagaa ggaatcaaag
      961 aaaatcctta ccaagtcgaa ctttatcacc tggcctcgga aaatgctttc cgtctgcatg
     1021 atagacagcg agcagaagaa cttttactga aagctttaga agtaggagac aaacaagatg
     1081 aaacattact tactttaagt aatctttact tggatgatga aagatatgaa gatgtgatac
     1141 aagtgatcaa tcagatggaa gaaactgcta atccttatgc agaatggaac ctagcccatg
     1201 cttataatga attagaagac tttaccgttg cagctgtaca ctatgaacag gcttatcatg
     1261 aactatcaca tgaaccagat ttcttgaaag agtatgcctt tttcttaaga gaagaagggc
     1321 agctgaaaag agcgcaagaa ttattgactt attatttaac acttgaacca ggagatatgg
     1381 aggcactgtc attattagat gacctaacag agaggtgaga aaaatgctaa ttgatgtacg
     1441 ggaaaagaag aattttttaa cgtggatggt caatcatgta tcttttagcc gaagagaagt
     1501 tttttggatc ttgaattatc ttgccaatca tgaagcgatt ttaagcaatg tccactttgt
     1561 tgaaggggcg aattttgcgg atcgaggact gcagatcagt gatctatctg ttgaagggga
     1621 acccatgaaa ttgtttttgc agggaaaaga atttacggac acagaccaga tttttcatga
     1681 aatccgtttg aattggaaaa agcctttata tgtggaatgt atattcgaaa atgcttggca
     1741 gacaagagaa tatctatcta tcttagaaga caatccattt tcttcttggg attcttcagt
     1801 cagtgaagaa gatactgaat taattgaacg atattttcaa gaaaaagaag aagaggcaaa
     1861 actaaaaatg ctttatgctc aaatcgatca agcactagaa gatggagata gggatgcttt
     1921 tttgaaatta tctgatgaag ttaaccgaaa gatccttttg cagacacaaa agaaaagtaa
     1981 ccgggtaagt gaacataaca attagttaaa aatagtatag tactaaaaaa gacaaaggtc
     2041 agctggatgt ggaatctttc ctcatagcct atctttgtct tttttcttat agttttttca
     2101 gtaatcttgt ctgtggcttc aaataagtaa agccttgccc ttccacttca tgcacattga
     2161 tgattgaaag gaaagctttt tcgtcaatct cgtggacgat cctcttagct tcagttaatt
     2221 cactagagct aacgaccata tagatgattt tttttgaatt accagagaat gcgccttctc
     2281 cagaaaggaa agtcacacct ctttgcagac cggtcatcag tagtggtgca atctcttcgc
     2341 ttttgttgga gacgacaagt agaccttttg cagaatatcc gccatctaag atcgaatcaa
     2401 tcacacgact gaaaacatac gaaacaatca acgtatacat catacgtttc aagtcgatat
     2461 aggttaatga agcaagtaag acgatgatat caaatgccaa tagggaacgc cccatagtga
     2521 tgccgtaatt ttgttctaaa atccgagcga taatatcact gccgcctgtt gttccaccca
     2581 tgcggtagac gatcccgctt cctacacctg caactaaacc cgcaagtaga gaaacgatca
     2641 gtagatcatg ctgtaaattg atttctagtg gaaccctttg ccagatccat aaaaagaatg
     2701 acaaagaaag tgtacctaga acagtatagg ccaaggaacg tttaccgagg atctttcctc
     2761 ctagtaaaat caatgggatg ttgattaata aagtcgtata agctggatca ataccaaata
     2821 aagctcttaa gatcaaggtg atccctgaga ctcccccttc tgctaaatga ttagcgatgt
     2881 tcaagtaaac aagaccaaat gcataaatac ttgttcctat aatgataaaa agaacttctt
     2941 ttactgtttt tcgtttgacc atatgaattc cagctttcaa ttatgtattt tcacagaatt
     3001 tccatttaga aattccttcc taaatgtagc atggaaattc gattttctca attgctctta
     3061 tctaggattt gcgttaaagt tttggtaaga tagttttgga gggaaaaaga tggataaaaa
     3121 acgttcttta tacagtatgc agcaggaagt ggatgaatat atccagcaat tcaaggtggg
     3181 gtatttctct ccattagctc agatggctcg attaactgaa gaagtaggtg aattggcaag
     3241 agaagtcaac cacacttatg gagaaaaaag caaaaaggct tctgaaccag ttaactcggt
     3301 agcagaagaa cttggtgatg ttttattcgt tacgatgatc atggctaatt cattgaatat
     3361 cgacttaact gaagtttttg aaaaaaacat ggaaaaattc aatcaaagag accataatcg
     3421 ctttgagcgt aaggacgagt aaacaagatg aagtggtcct ggccttgaag actctgaaac
     3481 ggacttgtca aacagtcacc atgcaactga gtctttatct caggacaaga aacacgcagg
     3541 aaaggttgga aaaatgaaat taaaacaatt acctctggaa taccaaaagg caattccagt
     3601 tctgaaaaaa ttagagaatg caggatatga agcttatttt gtaggtggaa gtgttcgtga
     3661 tatcttgctg aaccagccaa tccatgatgt ggatatcgca acaagcgcat ttccagaaga
     3721 aatcaaacag ctttttccaa aaacgataga tattgggatc gaacatggaa ctgtgttggt
     3781 attagaaaat gatgaacaat atgagatcac cacatttcgg acggaatcaa cttatcagga
     3841 ctaccgtaga ccagatcatg tcgaattcgt tcgttcgttg gcagaagatt tgaaaagaag
     3901 agattttacg atcaatgcgt ttgctttgaa agaagatgga gaaatcatcg atttgttcga
     3961 tggtctgcaa gatctagaag aacagatctt gcgagctgta ggcaatcctc atgagcggtt
     4021 ccatgaagat gccttacgaa tgatgcgcgg gttgcgtttt gtcagtcaac tagggttcaa
     4081 attagaggaa gaaaccttct acgccatcaa agaaaaccac gagctattag gtaaaatatc
     4141 tgtagaacgt ataaatgtgg aattcatcaa gatgctcttg ggaaatgaac gggccgcggg
     4201 aatccatgca tttattgaaa cagagtgcta catttgctgt cccggtttga aagaagaagg
     4261 cgaagcactt ttacgttttg ctgaattacc atccgtccca ttgaaagaag agagccaagc
     4321 atgggcatta ttgatcagcc aactggatct aaaagaaaat gagattcgtt cctttttgaa
     4381 atcgtggaag tgttctaacc aaatgattaa agacgttcaa gcacttgtcc aaggactacg
     4441 acaacgagaa actggagctt tggaaccatt cgaattgtac ttcttaggca aaaaacatgc
     4501 actgcaagta gaagaattga tgatctattt tgacgaaaca cctcagatgg atgccgtagg
     4561 agaatcctat gatcgtttac ctatcaagtc attaagcgaa ttagcagtag atggtaaaat
     4621 cttgctagaa gaaactggaa aaaaacctgg taagtgggtt agtgaagctt tgcaattagc
     4681 tgaaaaagcc gtcgtagaac aaagaataaa aaatgataaa aaacaggttt tggatttttt
     4741 atcgaaagaa aaattgattt aaaaaacact agactgtatc tgctcagctt tgttggctac
     4801 ttaaagtggg cagatacgtc attagaagaa aaaacataag atgtgaccac ttacataaat
     4861 ttgtgctaaa ctgaggtagt acaagaataa attacatcat aaaggagtct ttttaatgag
     4921 taaagaaatg acagcgctaa aattttattt tagaaatgga gaaacctgga cgatcaaccg
     4981 ccgtcatatc ggcgatcttt ggatcaaaca aatcacaaca agttttggac gaatcaatgg
     5041 cagtgaattt gttgaaatcc atccgtgtgc tggatttaaa atcgaaatct tccatgaagg
     5101 ggacgcagtt gcgacacatg atatcaacct aggcggactg gaaatgggca tgtttaatcg
     5161 cgcattgaaa tacgaagata tcgagcgaat ggaaatcctt taccgaaatg gcacacctga
     5221 tcttgtgtac ttcccatatc tagataaagg aacagaagga ttagacaatc aatatcaatc
     5281 aacaaaaatc agcgaaaaaa caggtaactt gtatatcgtg atcaatccag atcaacgtgt
     5341 agaagatgta tacggggaat ttttcgaata aatcgatttt tacgcaaaaa tgaggctgtg
     5401 aaagagatgt gtcttattac tactgatagg ctgtctcttc cacaccgttt attcagaata
     5461 agggactgtg acaagatttt tgtcgcagcc ccattttttt gttgtgataa aagaacaaga
     5521 aaggcaggaa tgggaaagta aacatccttt tgtcagaaac ttttatcttt cttaagtttt
     5581 acttgatatc tgatactatt tggaatagaa tgaggcgttg aaaaatttat ggaaatttct
     5641 aactagcaat aaacgatgaa acaatgaaat atttttcatc caaaaagaac tttgctataa
     5701 tgatggaagt ttgtgtatag gagatctgga aaatgagaga gagttttgaa caacaaaaaa
     5761 aactgttgca tgatcgctat ggcgcccttt cgatggatga ccgccgtcaa atactttgta
     5821 agttgcgtaa aagaaatata ttgatgtatc gtcagttgga acgtctgaaa catgatctgc
     5881 ttcgtttgga gtcaaaacgt gtacagtgtg aattagaggg gaaccaaaca caggttgaag
     5941 tagtcgaaac caaaatttta aagaaaaaag aacaattttt aaaaatgctt acacaaaata
     6001 aaaaataaag gggatggacg atggaatttg ctgagttagt tattgtattt gcagtaacga
     6061 tcaccttatc gaacatagct agccgcattt tgccaatgat tccagctcca ctgatccaaa
     6121 ttttccttgg agtggtatta ggtctgacag agtggggaca atcaatcgat tttgaacctg
     6181 aattgttctt agttatgatt attgcaccat tgttatttag agaaggagaa aaagcagata
     6241 tttcttcgat tctcaaaaat ttcgggacaa ttttgttctt ggcatttgga ggagtcattt
     6301 tgacccttgt cggggtaggt gcgacgttat cattcctgtt gccaagtgtc ccacttgctg
     6361 cctgctttgc ttttggagca gcgttaggtc cgacagatgc ggtagcagtc agttctcttt
     6421 ctggacgagt gaatatccct aaaaaggcga tgcatatttt ggaaggagaa ggtcttttga
     6481 atgatgcatc tggtgttact gccttccagt ttgctcttgg tgcgctgatc accggatctt
     6541 tttcagcagt aaacgcgggg atgagtctag tagtttctag tattggggga gctttgatcg
     6601 gctttttgct tgtctggttc aaacaaaaaa taattcattt gatcgaaaaa gcatcagcac
     6661 aagatgtcac agggtatctg ctgatcgaac ttttgcttcc ttttctagcc tatgtattgg
     6721 cagaggcatt tgatgtatct ggaatcattg ctgcagtggc agcaggtatt ctccaagctt
     6781 ctggttttag gaaaatctct gtgtttgatg cagaattgtc gagcttgtcc cacagtacat
     6841 ggacaactat tgcttttaca ctgaatgcat tagtatttat atttttagga atcgaattga
     6901 cgcaagtgtt ctcgccagta tggggagacg gtttatatcc aaacggtctt ttattagcga
     6961 ttatcgtatt aatctctgtc atgctttttg tgatcagatt catttctatc agtctttttt
     7021 atgtattcaa agatggcagt aaaaagttca aaaaacagtt gaacgagatc ttgatcttaa
     7081 catttggcgg tgtaaaggga acagtcagtt tggctacgat ttttattttg ccattttcaa
     7141 tcaataatat gatgttttac cagcggtctt tgcttttgtt tctgacggcg ggtgtcatct
     7201 tagtgacttt ggtcataggg atcattgtct tgcctatgct gacggaaaca gaggaagcga
     7261 aaagcacgga tttgaatgct ttgatgattt tagaagaggt cgttgaagtt ttgcgaaaag
     7321 aaataaagga aattgatcgt aatacaaaag aatttttagc cactgaagca gtgatagaaa
     7381 attatcaaga acggatacgt gatctgtact tggaagattt aaccgatgat gaaaagcaag
     7441 aagtacaaga aatacaagca ctgattttgt cgatcgaacg ggatggattg gatgaaagtt
     7501 atcgttcggg caagctttct tctaatggtt atcgttttta ctcccgtttt ctttctcgat
     7561 tcgaacattc tatcacaagc cagattttgt cttttatcgg cttttggttc attgttgtcc
     7621 gtaggttaat gcgaatcgtt ctccatccga aaatgttttg gcaaagaaga tatgctgatc
     7681 gtcagacatt tattagtgat gaagatatcc aagaaatcag agagacatat caaaaaaata
     7741 ctcagttgat tattgaaagt ttagataact tgactgatat ttatgatgaa acgatggttc
     7801 aattttttat ccagcaacgc aaacttgaag gaataaaaat gaactcgggg aatttgattt
     7861 cttcgtggat gatccagcaa gatagtctgt ttacgaaaaa aatgctaaga ggttattatc
     7921 ttgaaagaaa aatcattgat gaatacgaag ttgctgaaaa aatcacgacg ttttctgcga
     7981 acagctatcg caggaatatc aatttgttag aatcttatac gatgaacaaa ccatctgata
     8041 acttttcttt ccgatttgcg tttcgagcaa agtcaaaaaa aacgagtcca ataaaataaa
     8101 aaggtaaaaa ttttcaagtt ttataaaaaa caaaacatat cagaacagga tcgaatcggt
     8161 cctgttcttt ttttgagaac acaaaaagaa caaataccaa aaaaattttc ctgaagttca
     8221 ctatttttat gatacaatat ccaagataag gagggaacat gttgaaagaa ttaaaagtaa
     8281 ctgacctcaa aaaaacttat ggtgaaaaag atttgtttga ccagatttct tttttggtcc
     8341 acgaaaaaga ccgtatcggg ttgattggga cgaacgggac gggtaaaaca agtctcttaa
     8401 atattattgc cggtatcgat agcagcgacg gtgatcggca aacagtgttt tatccaactg
     8461 attaccgtat tggttatctt tctcaagcag ctgaattttc cgatgaatta actgttctcc
     8521 aagcagtctt tcaaggaaac agccccttga tccaaactgt tcgagcatat gaggaagcat
     8581 tgatcgaact aggggaaaac ggagaagacc ctgatgtgca aaaacgttat gctaaagcag
     8641 aagaacaaat gaataaagaa gatgcttgga ctacagatac caacgcaaag attattttgc
     8701 aaaaattagg aatcagtgaa ttagataaaa aaatcagtac tttatcaggt ggtcagaaaa
     8761 aacgagttag tctggcgcaa gtgttgattg atgaacccga tttactatta ctagacgaac
     8821 caacaaacca tttagattat gaagcgatcg agtggttgga aaactatttg aaacaatacc
     8881 gtggcgcact attgatggtt acccacgatc gttattttct agatagagta gcaaaccgaa
     8941 tcttcgaact ttcatttgga aaattatacg agtataaagg aaattatgaa gcttacgtct
     9001 tagaaaaagc agaacgtgat cgtgtagaag tagaacaaga agaaaagcgc aagcggcttt
     9061 ataaacagga attagcttgg atgagggctg gcgtacaggc acgtggcacc aagcagcaag
     9121 cacggatcaa ccgtttcgaa gatctaaagg agaacttgta ccaagtaaac caagaagatg
     9181 atttagagct gaacctagcg acacaacgtt tagggaaaaa agtactggaa atcaaagatg
     9241 gcagttaccg tataaatgac caaacactgc tagaacattt agatttattg attcaatctc
     9301 gggaaagact aggtattact ggtaaaaatg gtgcgggaaa atcgacattg cttaatattc
     9361 tagctggcag agcggtacaa tgtctatcgg tgaaacggtt catcttgcgt attatacaca
     9421 agaaaatgaa gaaatggcac cagataaacg gatgattgct tacttacaag aagctgctga
     9481 agaagcaaaa acagcagatg gcagtcaaat cggagtagca gaattacttg aacgattttt
     9541 atttccaaga tttatgcatg ggacactgat aggcaaactt tcaggcggag aaaaacgccg
     9601 gttatttttg ttgaaactgc tgattcagca acctaatgtt ttattattgg atgaaccgac
     9661 caatgatttg gacatagcaa cattgacgat tttggaagat tattttagaa gttttccagg
     9721 tgctgtgatc actgtttccc atgaccgtta tttcttggac aaggtagcag ataagttatt
     9781 agtattccaa ggaaacggaa aacaagaact ttattacgga aacatgtcca gttatttact
     9841 gaagcaaaaa gagacccagc agcctgctga aaaagcgaaa ccaaaaatac agagcaagga
     9901 acctgctgaa aagaagaaat tatcttacat ggaacaaaaa gaatgggaaa cgattgaaga
     9961 tgagattgca gaacttgaag aaaaaatctc attgctccaa gaagagatga atcatcaagg
    10021 agataatttc actcgattac aagaattgca aaacgacgta tctgaaacag aagcacaact
    10081 tgaagaaaaa atggcacggt gggaatactt gagtgaatgg gtagaagatt aggaggaaaa
    10141 aggatggaac aagcgtatct tgacttagga agaaaattgt tggaagaagg gcatcgtaaa
    10201 gaagacagaa caggcacagg cacattaagc ttattcggat accaaatgcg gtttgatctg
    10261 caaaaagggt ttcctttgtt aacaacgaaa cgagtacctt ttggattgat caaaagcgaa
    10321 ttgctttggt ttttaaaagg agatacgaat atccgctatc tattacaaaa taaaaaccat
    10381 atttgggatg aatgggcatt tgaacgctac atcaaaagtc cagactataa tggaccagat
    10441 atgactgatt ttggccatag atgtttgaca gatcctgaat tcaatgaaga atataaaaag
    10501 cagagcaagc ttttttgcga gaagatccta accgatgatt cctttgctga aacatacggc
    10561 gatcttggac acatctacgg ctatcaatgg cgtcactggg aaacaaaaga cggtggcttt
    10621 atcgatcaaa tcaaacaagt gatcgaagca atcaaaaaga ctcctgattc tagaagattg
    10681 atcgtttctg catggaatcc ggaagatgtt cctagcatgg ctttgcctcc ttgtcatacg
    10741 atgtttcaat tttatgttca agagggacgg ctaagctgcc aattgtatca acgcagtggg
    10801 gatgtgtttc taggcgttcc gttcaacatt gcaagttatg cgttgctgac tcacctaatt
    10861 gcacatgaaa caggtttagc ggttggcgaa tttgttcaca cattaggaga tgctcatctt
    10921 tatatcaatc atatcgaaca aatgaaagaa caattgagcc gagaaatacg cgcatttccg
    10981 actttagttt tgaatcagga aaagcagtca gtatttgatt ttgagatgga agatatccaa
    11041 ttagaaggat atgacccaca tccggcgatc aaagctccta tcgctgttta gaatcagaaa
    11101 ggatgagaaa atgttagctg caatttgggc acaggatgaa aacggattga ttggaaaaga
    11161 agaccgtctt ccttggcgtt tgccaaatga tctgaaattc ttcaaacaaa tgacagaagc
    11221 aaatacacta gtgatgggac gaaaaacatt cgaagggatg ggtagccgtc cgttgccgaa
    11281 tcgtcagacg attgttttga cgagagattc ttcctataaa gcagaaggcg tgcatgtcat
    11341 gcatagtgtg gaagacgtgc ttgctctgga aaaagaaaca gacggtatct ttttcatcgc
    11401 tggagggtca gctgtttatc aagaatttct gccattttgt acgatattat accgtacagt
    11461 catccatcat gtattcgatg gagatgctta ttttccacct gttgactggt ctgattggtc
    11521 actgatcaat actagtcaag gagaaatgga tgaaaaaaat cgttatccgc atcaatttga
    11581 aacgtatcag cgtaatgaga atcctgtaaa aagcgggaca ggcgaattga cgatataaaa
    11641 aagctatgga tagactagct gttatttaga gctgtcccat ccatagcttt atttttttta
    11701 ttaagtaaag aataaaacag agaagtacat aaaacctgca cctaacataa caaacaaatg
    11761 ccaaacaaca tgcatatagc ggatagaatt caagctataa aagaatgcgc ctatcgtgta
    11821 agaaagcccg cctgctacca tcaaaataac accagtcatt ccaagagagt gataaagagg
    11881 aacagcagcc acgacacaga gccaccccat aaaaatatag atgatcgtag atacttttga
    11941 tactctctcc cgtttatgca acgtcaacgc tttatatata atgccaagaa tcgcaaacaa
    12001 ccagattaat acaaataaag tccaacctag ccatcctcgg atactcagca aacaaaaagg
    12061 agtataactt cctgcaatca aaagaaaaat cgaactatga tcgaatactt ggaacacctt
    12121 ttttgctttt gtaaaaatca atgaatgaaa caaagtagac gttaaaaata aaagaatcat
    12181 catcgatcca tagatagcat aagagacaat atggagggag gaatgcaagt gggcaccttt
    12241 tactaaaaga accactagtc ccgcgatact caatccagtc ccaattccat gggtgactgc
    12301 attaaacact tcatttaata tttcatacgt tcgattaaat ttcgtttttt ccatgaaaac
    12361 ggctccttta ctaggtgtaa ttaatgaaaa atcattattt gtctgttata ctaaaaatag
    12421 aaattgtctt acatgtagtt taacatgaat aaggcatctt gttaaagaaa gagagagaga
    12481 aaataatgac aaatgtaaaa atcgtaacag attcttcatg tacgatggaa aaaaatgtac
    12541 gcgatgcttt aggcattcgt gtcatgccgc tatccatcat gatcgacgga acagtatatc
    12601 ccgatgatga ccaactagac ggcgaaagat ttatggaaat gatggcgaaa gcaaaaaatc
    12661 tgcccaaaac aagccaacca ccaattggag aattcatctc gatgtatgat gagctaggag
    12721 aagacggcag tgaagtgatt tcgatccata tgacaaaagg cttgagtgga acagtagaag
    12781 cagcaagaca agccagtaat ctaacaaaaa cgaaagtaac ggttatcgac agtgatacca
    12841 ctgaccaagg attatctttt caggtaattc gagcagctga attagcaaaa gaaggtaaaa
    12901 cagtagagaa aatccttgaa gaagtcaaaa aagtacaaga caatacaaaa ttatacatcg
    12961 gaatatccac actagataac ttggtaaaag gtggaagaat cagccgaaca actggtttgc
    13021 tgtctagtgt tttgaacata cgcgtggtga tgaatttcgc ccattctgaa ttaatccctg
    13081 taactaaggg gcgaggaaag aaaacgttcg ataaatggtt taataatttg aaaaatgaac
    13141 tgaaagaatt gccaaatgtg cgacaaatcg gtatttcaca tgcggatgca aaagaattgg
    13201 cgcaagaatt caaagagggt ttgcaagaat tattcccgga catgcatatc cctgtactgc
    13261 atacgaatcc ggttatcgct acgcatactg gtaaagacgc gtttgcaatc atgtattatg
    13321 tagattagtc gaaaagagga taagccctca gcctgaaagg cttgcagctt attctctttt
    13381 tctttataaa attaggaaaa taattgtttt cttttttcat aaagactacc tttttgataa
    13441 aatttttgat aacattagaa ccaatcgaat acaagaggtg aaaaaatgaa attaacgaaa
    13501 cgacatttga tcctcgtttt gttcccatta ttaattgcga tcatcatcta tgcaggttta
    13561 gctgcttcta taccgaaagc tggcccacta atgaaaccag taactactgt gagagaagat
    13621 ccaaataaaa aagaaaaact tcactacata gctatcggag attcgctgac tgaaggagta
    13681 ggagatcaaa ccaagcaagg aggattcgtt ccccttgtcg caaatgatct aaaagaccgt
    13741 tataatttga ccgcggttga aacagaaaac tatggtgtaa atggtgaacg aagtgaccag
    13801 atcttaaagc gagtaaaaac aaaagaagaa attcgaaaca atatcaaaac agctgatttt
    13861 atcacactga ctgttggcgg taatgatttg atgaaagtta tccaaaataa tttattcggg
    13921 ttaacggtca attcctttaa aaagccactg aaaaactatc aggaaaatgt cacagaacta
    13981 ttggaagaat ttcgatccct caataaaacc gcacctattt acattttagg catctacaat
    14041 ccattttatt tgaactttcc tgagatcact gacatgcaga caattgttaa caattggaac
    14101 aaaggaacag aaacggtgac aaaaaaaatg ccgaactgct actttatccc aatcaatgat
    14161 ttgctttatc aaggattgaa tcaggaagta ggtgtcacag aagaaggaac agatcaaaca
    14221 actagcacga cagaaaatct aaatggcatc aaaaataatg tgctctatga agaagatcat
    14281 ttccatccaa ataatttagg ttatcaattg atggcaaatg cagtgagaga tcagctgatc
    14341 gagacacaaa accaatggtt agagaaggag gatgatcaat aaacatgaaa acaagatctg
    14401 acgtatcttc aaacaaaaca aagaaaacaa attactggaa attcgccttc cttatccttc
    14461 ttggggtagt acttggttcg accgtatttt taggcagccg tatttttgct aatcgggaac
    14521 cagaactccc agaaatacca gctcttacgg aaagacaagg agatcctgtc ttaacgatca
    14581 attcaaataa agaaaaagtc aatcaaatca tcagcttttt ccttagtgag tatcaaaaag
    14641 atagtgatat tgaatataaa ttttatttgg aaaatgaagc actactcaat ggtactttcg
    14701 aagtactagg ttttcctatc gatttctatt tatattttga tccgtatgtg atggaaaacg
    14761 gaaatgtcca actaaaagct agaagtcttt ctatcggaac attgagtttg ccaatcaaag
    14821 atgtgatgaa tatgatcaag agaaactata aattaccaga ttggatcgaa gtcaatacag
    14881 atgatctgac tatcatgctt aggttggatc aattccgaat gcaaaacggg atgtatatta
    14941 aagcagataa aatcgactta gtaaatgatg acattcggtt tagtctatat ttgccaaaag
    15001 atacctctga tgcttcaaac aactcaaata ccaacaaaac tagcaccagc agtactgaaa
    15061 gcaagacaaa agaaaataaa aaatcatcgg atgagtagta ttagaggggg aatcataatg
    15121 gaacgagcaa tatttgcagg aggctgcttc tggtgtatga tccagccttt cgacacgctt
    15181 cctggtatcc atacagtgat gtcaggatat actggagggc atgtaccaga tccgacttat
    15241 gaacaagtaa aaagccaaac gactggacat acagaagcag tggagattat ctatgatcca
    15301 acattgattt catatgaaca attagtagaa ctttactggc agcaaactga tccaactgac
    15361 gctttcggac aattcgagga tcgcggtgac aattatcgcc cggtgatctt ttatttgaca
    15421 gaagaacagc ggatcattgc tgaaaaaagt aagaaacagt tagctgaaag tggacgtttc
    15481 gaacagccaa tcgttacaaa aatcgaacca gcccaaccat tttatctagc agaagaagac
    15541 catcaagagt attataaaaa gcatcctgaa aattttaaaa gaaaccatgc acgccgggct
    15601 gcctttatcg aagaaaattg gaggaacgaa gggtgaatcg aagctttttt cattatttaa
    15661 tgacactgcg aggcggtggc acacatgatg ctttatctgt tttcgctaca gaagccggta
    15721 aagacatcca atttcctaag cacagctcta gctacgaaga aatttctaat tatctggaac
    15781 tgaatgtcga ctatttgccg tccatggata tctttgacga agcatgggaa aaatatttag
    15841 aaaataatca tgacatataa gagatagttt tgtactttat gatcaaaaaa gttttttgtc
    15901 caatagccta attgaaagaa ttatttttat ttcagctatc tagtcgcatt tttttgatat
    15961 ttttcttata ataaagaaag aatgaaaaaa agagggatgg attgatagag attgacaaaa
    16021 aaacaacgct atattaatcg cttctggctt gtattcaaat acttacttgt ttctgtattc
    16081 cttgcgttca tgttgcgggg atttttattc atcccggtac cagtggaagg caattcaatg
    16141 gaaaacgtgt tgaagcaagg tgatatggtt gtgatggaaa aattttcgga gatcagacga
    16201 tttgatattg ttgtttttca attagcagat ggcacgattt atatcaaacg agtaatcggt
    16261 cttccaggag aaaatgtcag ctaccaaaat gatcaattga agatcaatgg gaaagtagtt
    16321 aaggaacctt atctaactaa aaacctgaaa tcagatcatg caaatgcatc ctatacgacg
    16381 gactttacac tacaagagct aacgggacaa tccaagcttc cggaagataa ttattttgta
    16441 cttggagata atcgaagagt ttcaaaagat agtcgttcgt ttggcacgat caataaaact
    16501 gatattctag gtaaagcaag atttgtctat tatccacttg atgaaataaa atggatccag
    16561 tgagaaacaa taaagcgtgc agtctgttga gactgcacgc tttattgttt aatccaaaat
    16621 cagtttacta ccttagtacg taaaatcaaa cagctcgttt aaacactttt ctagcctcta
    16681 aagttttgat aaatgaataa acaaccatta tttgaatagg gatttggatc atattcctga
    16741 tgaatctacc tagaaaaatt actttgaaat ctgcaccaaa caaaacagac atcagccaag
    16801 aattgataaa aaggctgact gggatcataa tcaataattg ggcagcgatg acggaaggca
    16861 ggtttatctt tttatgatat aaaaataagc cattaataaa tccaccaagg aaagccgtga
    16921 taagaaaagc agcttgaaac gttcctgaac caaatatcgt gaaatttaaa gtgttcgaca
    16981 acatttcaag caaaccagcc cagatcggtc catatagata acccataagc gccatactta
    17041 aaaatccgaa tgtgatttga aaataagcag gaccaatcgc tatcttgtat aagatgacat
    17101 caagggctaa taaaagagca gaggtcgaca acaatcttgc attcatttcg ggcaatctaa
    17161 aaacgtcttt tgcaatcaaa gtgttcaaaa tattctctcc tttgtcagtt aataataaaa
    17221 ggataccata aa
//
LOCUS       DACKAC010000059        17005 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.060, whole genome shotgun sequence.
ACCESSION   DACKAC010000059 DACKAC010000000
VERSION     DACKAC010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17005
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.060"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..124)
                     /locus_tag="I0B46_002058"
     CDS             complement(<1..124)
                     /locus_tag="I0B46_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HAR2084332.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVF"
     gene            complement(228..539)
                     /locus_tag="I0B46_002059"
     CDS             complement(228..539)
                     /locus_tag="I0B46_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAR2084333.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(541..738)
                     /locus_tag="I0B46_002060"
     CDS             complement(541..738)
                     /locus_tag="I0B46_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAR2084334.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(1132..2859)
                     /locus_tag="I0B46_002061"
     CDS             complement(1132..2859)
                     /locus_tag="I0B46_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAR2084335.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2852..4045)
                     /locus_tag="I0B46_002062"
     CDS             complement(2852..4045)
                     /locus_tag="I0B46_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAR2084336.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(4232..4876)
                     /locus_tag="I0B46_002063"
     CDS             complement(4232..4876)
                     /locus_tag="I0B46_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAR2084337.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4970..5104)
                     /locus_tag="I0B46_002064"
     CDS             complement(4970..5104)
                     /locus_tag="I0B46_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAR2084338.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(5106..7238)
                     /gene="feoB"
                     /locus_tag="I0B46_002065"
     CDS             complement(5106..7238)
                     /gene="feoB"
                     /locus_tag="I0B46_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAR2084339.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(7235..7708)
                     /locus_tag="I0B46_002066"
     CDS             complement(7235..7708)
                     /locus_tag="I0B46_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAR2084340.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            8109..8333
                     /gene="nrdH"
                     /locus_tag="I0B46_002067"
     CDS             8109..8333
                     /gene="nrdH"
                     /locus_tag="I0B46_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAR2084341.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8492..10651
                     /gene="nrdE"
                     /locus_tag="I0B46_002068"
     CDS             8492..10651
                     /gene="nrdE"
                     /locus_tag="I0B46_002068"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAR2084342.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10701..11666
                     /gene="nrdF"
                     /locus_tag="I0B46_002069"
     CDS             10701..11666
                     /gene="nrdF"
                     /locus_tag="I0B46_002069"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAR2084343.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11755..12894)
                     /locus_tag="I0B46_002070"
     CDS             complement(11755..12894)
                     /locus_tag="I0B46_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAR2084344.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(13203..13916)
                     /locus_tag="I0B46_002071"
     CDS             complement(13203..13916)
                     /locus_tag="I0B46_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAR2084345.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(14170..14871)
                     /gene="nagB"
                     /locus_tag="I0B46_002072"
     CDS             complement(14170..14871)
                     /gene="nagB"
                     /locus_tag="I0B46_002072"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAR2084346.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            15105..16637
                     /locus_tag="I0B46_002073"
     CDS             15105..16637
                     /locus_tag="I0B46_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAR2084347.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16828..>17005)
                     /locus_tag="I0B46_002074"
     CDS             complement(16828..>17005)
                     /locus_tag="I0B46_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAR2084348.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5278 a         3495 c         2909 g         5323 t
ORIGIN      
        1 aaaaaacaca ttccactaaa tttgatgccg gttttccttg aggttttgtt ccagcacctc
       61 ctttaatttt tttaagctct tgatctgaac attgtttagc atttagtcta tttttttctt
      121 ccattacgat tccccctaca aactataaat acttttatgt cactgattca atatacctat
      181 ttttctaatc aaaaaatttt gagacatatt taatattata agtatcatta aaattgagat
      241 ttatctccat aatctgctcg atataaacca tttattccag cagttcttcc aatttcactc
      301 aaatttctta ttaattcctc ttgatattca gtgaatctta ttttattagt ataagcagta
      361 aaatatagat aattcaccaa tctatttatt aaaggactgg gatctaattc atatttttgt
      421 ttttctaatt ttaaatatac cttttgtaaa acctcaagaa tatcagaata tttaggatct
      481 ttacttatag atatatcatt atataattca tgaacaattt gcttagcatt ttttttcatt
      541 ttagcacttc cctggaattg ctccacctaa aaaaccacct atagacattc ctgcaataca
      601 agtagttgcc ttggcccaat cgaccgtaca tttattttta gtgcaataca ctccatttcc
      661 ataatatttt ccactatgag tggtaccccc atagataagt tgtgtctctt taatagacaa
      721 aatttttaaa tgtttcataa tctaatcacc cctaaattaa tgtttttaga ctattattct
      781 aacattcatt aaatttcaaa aaacaattcc ttcttgaaac gtagcaaaat aattcctgaa
      841 taagaattta atcactttta agtatataaa ctatgtatat tctttaatat tttttgagaa
      901 tgcacttcgt acataataaa ctttaatcac attagtcttt catacctacg atcgatctct
      961 ttgactcgca cccataaatt cataatagtt tgtcggcgtc tttgaacgtt tcgaagatga
     1021 gctacgcatt ctcttttttt atagacaaaa aagatagaga cgaacatgga gcttccaaat
     1081 ttggatattt gatccaattt tggaagctcc atttatttcg tgtctgtctt tttatttttt
     1141 ccagctttcg actaattctt ggttctcttt gatccagtct tttgctgctt gctgtggatc
     1201 ttttccattg ttgatttcca acataacttt ttccatatct ttttctgacc aatggaaatt
     1261 gtctaatact ttgtaagctt ctggctggtc ttctttcaat ccttttcgaa ccatcgtatg
     1321 gatctgctct tcgcttccca ttccgttttc aggatcttct aagtatttca gatcatattt
     1381 ggcaaacatc caatgaggtg tccatcctgt aactacaatt ggttcgtgtt ttttgatcgc
     1441 ttgacctagt gcaacggtca ttgctcctga agaagatgtc tctactttcc agtctttcaa
     1501 gttgtcatat ttttggatcg tgttttcagc agccgtaact acaccagctc ctggctcgat
     1561 tcctatgatt ttcttacctg cctgatcggt taactcatcg atcgaattta catccatgta
     1621 ggctggcact gctaaaccaa cttttgctcc tgtcaaattg gcacctaggt cttcgacttg
     1681 atctttatat tgtgcatatt gtgaaccatg agttttaggt agccaagctg aaaccatggc
     1741 atctgactcg ccatttgaaa cagatttcca catgattgaa ttatctaatg gcgtcatcgt
     1801 tacatcatag cccatttgtt tcaatacttc accgactaca tttgttgaag caacttctgt
     1861 atcccattca acataagata agttgatacg ttttgtttct ttggcagatg aaaaactccc
     1921 tataccaatc gcacctaaga tcactactac tacagcacca atgatcagtc cttgacgttt
     1981 tttacttttc tgtgttgttg ctcctgcagc ttttttattg tttggctggt tcaaatgctg
     2041 tgtaaaacgg tcaatgatga ttgctaagat aactaatgca accccgttga caaatccatt
     2101 cccgacttgt gctctttgca atgctgataa tacgccgcgt cctaagccgg gtgcaccgat
     2161 catagaggca ataacgacca ttgaaagtga cagcattgtt gtttgattga tccctgccat
     2221 gatcgtactt ttagctaaag gcaattctag tttgaatagt ttttgacgac ttgtgctccc
     2281 gaatgaatca gaagcttcta ctaattcttt aggaacttgt cgaatcccta aattcgtaaa
     2341 acgaacggtt ggtggcagag caaagatgac tgaggcaaat acccctggaa ccatcccgat
     2401 accgaaaaat gcaactgctg gaatcaaata aacgaatcct ggcattgtct gcataaagtc
     2461 aaggatcggt ttgataatac tttgggcttt actgcttttt gccatcaaga tccctaatgg
     2521 taccccaatg atgatcgaga tcacactaga taaaagaaca agtgtgacag tattcatcaa
     2581 atcattccat agaccttggt tataaataaa ccatagaccg attaatgtga acaacgtaag
     2641 tcctggacgt ttcttagaaa taaagaaagc tgctaccgtc aataacaaga taaacaaagg
     2701 agctggaatc actaacagta aatttgtaat ccccgacatc actgcctgtc caatcgtttg
     2761 taaaaatgaa aataggccag aaaaagttgt cgtaaaccat tctgtgatat tttctaccca
     2821 gtttgccact ggtaattgat agttcaatag attattcatg ttcactcacc tcgcttgttt
     2881 ctgctaatgc ttcaatcaca ctacctcgga tgacgacacc ttttaatttt ccatcctcta
     2941 caactgcaag tggagtcggt gagtcataaa taatattgaa aatatcactt accaccatgt
     3001 ctttcccaat caccgtcaca ttttcatcta ggaagtcgat caatggacgt ttttgcttcc
     3061 tagcttctaa cgcttgatct gcagtgatga tccctttcag gtgtctcttt ctgtcaacag
     3121 ccatcagcat gctgacttct tcattacgca tccttgtcaa tgcaacgttt ggtccatcag
     3181 attcgatatt cgtcgtcaat gcaggtacca tgatgttttg agccgtaagc actttggaac
     3241 gatcgacttc ttccacaaat tcacgtacat aatcatttgc tggattggtc aatatttctt
     3301 cacctgttcc gatctgcatg atttcgccgt ctttcatcaa ggcgattctg tccccaatcc
     3361 gtaaagcttc attcaaatca tgggtaatga aaataatcgt tttttgaaca ttggcttgca
     3421 ggtccagtaa ttcgtcttgc atttctcgcc ggatcagcgg atcaagagcc gagaacgctt
     3481 cgtccatcag caagatctca ggatcatttg ctaaagctct ggctaggcca acccgttgtt
     3541 gcatcccacc agataactga ctaggatatt ggtctttaaa tgaaagcaaa ctagaatttt
     3601 ctaatgcttt ttctgctttt tcttgtcgtt cttctttagg tacaccacgg acttctaacc
     3661 cgtattctgt attttccaag atcgttcgat gtggaaacag accgaagttt tggaaaacca
     3721 tattgatttt atgacgacgg acttctcgca attcttcttt gcttaacttc gatacatctt
     3781 ctccatcaat atagatactg cccgaagttg gctcaatcag acgattcaat agacggatca
     3841 atgtggattt cccactcccg gaaagcccca tgatcacgaa gatttctcct tcttttacgt
     3901 caaaattcac gtcatacaca ccaaccgttg caccggtttt ctttaaaatc tccgttttcg
     3961 gctggtgatc attcatcata tctaaagcgg cttgtgtctt tttaccaaat atctttgtta
     4021 aatgttttac ttctactttt accaaacaaa ttctcctttt ttatgtaaat tcatttttct
     4081 ttctgaaaaa agtgaccaca ctatgttaac atcatgtagt cactttgtca aaaatgaaag
     4141 aaatttgctt gtccaaactg aaaaaaactt ggctaaatct ttgtaaataa aaggtttagc
     4201 caagttaata tttttttaat atttcacaca atcattctgc aagctgatgc tccgggtaaa
     4261 aaaagtttgt catacgctgt agtgtatatt catttccaat aaaatgaacg atgtcccctg
     4321 ccgtaaattt agcatagggg ccaggtgaaa caagcagttc ttcattatga gaaattgcga
     4381 taactgtggc gccagtcgcc tgccagatat tcaactcgct aattgaacgg tccagatgca
     4441 ttgctttttc tgataaagat aattcaaacg gaagtaaagg attcatctta tgtattcgct
     4501 tcgtttgatt gaccagctga tccaataatt gagaaaaatg attcagctca tcttgttgct
     4561 tctccacact tgccatgatt tctgcttttg tatcttggat ggattgtaca tcttgatatt
     4621 gcaataggaa atcttttgct ttttccttcg aagcaacaaa tgccccactt ccatgccgta
     4681 cttccatgat tccaagatct accagcacat tgatagcctt gcgggtagtc tcaggagaag
     4741 cattgtacgt actggcaagt gttgagcggg catggatctt ctcacccact ttatatcggt
     4801 tttcaacgat acgttcggct aaatcaacag caatctgctg gtagcgcggg cggacgattt
     4861 ttttctttcc agacattttc tttcctcttt ctttttttac tttccctcat cttactatgc
     4921 tctagcaaaa taagcaaaaa aggggagaat agcctcttgc aacgactgat catcgcttga
     4981 tctgttcctt ttttactggg caagctgttt gacagtcatc acaattctta ccggatttta
     5041 aacgagtata gacaattgcg cctgcacttc caaagattaa cacacttaat aagaatgtcg
     5101 ccatcctatt cctctccttc taatgacaat atagtataaa actggttctt ggattttggc
     5161 tgacggaaca aaacataaac gagtcctatc atcagtaaaa tagcaagata cgtccctaac
     5221 gctacctgtc caccctccaa taagtgtccc aactgataaa tcacaaaact aactaaataa
     5281 gctaaaccgc attgatagcc tatggcgatc caagtccatt tcttggcatt catttcccga
     5341 tggatagccc caattgccgc aaaacaaggc gcacaaagta aattgaaggc taacaatgaa
     5401 taagctgctg cgggagtata tgtgtgttgt agcacttgcc agatttctct gccattttcg
     5461 gatacttccg catgaccaaa caaaatacca aatgtgccaa taatattttc tttagcgacc
     5521 aatcctgtaa tcgctgcgac tgttccttgc cagtttcccc aaccaagcgg ctgaaaaaca
     5581 actgccagga ttcgcccgaa aaaggctaag atactttggt cttcagaaac aggttgagca
     5641 taaaaattat aagaagagct gaaccagatc acgatactag aaacaaagat aatcgttcct
     5701 gcttttttaa caaaagactt gctgcggtca taggtttgtc ttaggaccgc tgaccattga
     5761 ggaaaatgat atgcgggcaa ttccatgata aaaggagctg tatctccagc aaacaaacgt
     5821 gtttttttca agctgattcc agagaaaata attgcagtca taccaagaaa ataggcagaa
     5881 ggagctaccc aagattgatg aggaaaaaag gcccctgcta ctaaggcaat aatcggcaac
     5941 tttgcagaac aaggcatgaa ggttgtgacc atgatcgtca aacgacgatc ttgctcgttt
     6001 tcaatcgtgc gactggccat gacgcctggc actccacatc ctgtagcaat cagcatagga
     6061 atgaaggatt ttcctgaaag accgaatttc ctgaaaagtc gatccatgac aaaagcgatc
     6121 ctcgccatat atccgcagtc ttccagaaag ctcaagcata aaaacaatat aatcaattgc
     6181 ggtagaaatc ctaacactgc tcccacgcct gcgatgatgc cgttcaagat cagctcctgc
     6241 atccatcccg ctacttgcca ttcagctaac aatctgccaa catgttctgg taaccatgta
     6301 ccaaacaatg tgtcattgat ccaatctgtc cccattgttc ctactgtttg aatagccaga
     6361 taatagatca gccacatcac aaatgcgaag atcggcaagg ctaaccaacg attcgttgca
     6421 atctgatcga ttttgtcact catcgataat tggaaagaat catttgacac aacacagagt
     6481 gtacatagtt gggtaataaa ttcataacgt tcattgacca gaatcgtctc actatcgtcg
     6541 tccaatagtt tctctgttaa ctggatcagt tgctcgatct ctttttgctg agaaactgct
     6601 atctcgtaat cctctcttac cttctgatct cgttcaaaat atttcattgc ataccagcgt
     6661 ttcttgccgc tttgttctcc tagtacatct tcaatttcat gtaaagctgc ttctaaacgt
     6721 ggatcatatg tacagggaac aggaatagaa ggccgctttt ttgcagactc ctgaatcttt
     6781 tcgattagtt tttcgattcc ttgctttttc aatacgctga ttggtaaaac tggtacacca
     6841 agaccatagg aaagcttttc tagattgatt ttttttcctt cttttttgat gacatccatc
     6901 atattcagac caatgatcat aggaatgtct aaatccaata actgcgtagt taaataaaga
     6961 tttcgttcta aattggtagc atcaataatg ttgatgatca gttcaggctg ttcctgaacc
     7021 aaataatctc ttgcaatcac ttcttctggt gtataaggag acaaagaata gatgccgggt
     7081 aggtcctgaa tatgaatgtt cggatttttt ttcaacgatc cttctttttt ttctactgtt
     7141 acgcccggcc aattccctac atattgattc gaaccagtca agagattgaa caagctagtt
     7201 ttcccgctgt ttggatttcc gactaacgcg atattcattc tttttcctcc ctttgtatca
     7261 agattagttc ggcctctgac tttcgtaacg tcagttcata ccctcgcact gtcagttcga
     7321 ttggatctcc cagaggggct agttttcgga taactatttt tacatttcgc gtcaatccca
     7381 tatccattaa ccgtcgcttc actgcgcctt gtccgtggat tgctacgacg atcccagtct
     7441 ctcctgcttt taattgatca agagaagtcc agctttcaga ggaagcagtc tttttattga
     7501 caaagatttg acctagtatt tcccgactta ctgccactcg atgacttttt accagtaaaa
     7561 tggcattgtt gcctgataaa ttgacgacga cgattttact tccggcaacg atcccgatat
     7621 tttgcaaatg gcgttttgtt ttttcttctg tttcgattcg ttcgacttga taaacgaaat
     7681 tctgctttgc ctgcgctaag gtctgcatat tgattcctct cttgtgaaat aaatcatgta
     7741 ttaaaatcat tcattatatg ttctaatttt cttacgtgtt acgcaaataa atgagaatca
     7801 ttctcacata gaatactctc ggtaattgag aatgtcaatt atacttgtga tattatgagc
     7861 gaatctttag tattgataga taaaaatgag aaaaaaatat ttcttcatag ttttccacaa
     7921 ttttttctct attaaatttg ctattttccc acacgatact tgttttcagc tcttttttca
     7981 aaaacacaaa acttagtatt ccacagtttc cacaacacaa tatgtagtgt ttgttgtttg
     8041 acttttttac taagagtttt atactatcta ttatagataa taagaatgtg ttagaggagg
     8101 cactcaccat gaacgtaaaa atcttttcta aaaataattg tatgcaatgt aagatggtca
     8161 aacggttttt aacagaaaat caaattgctt ttgaagaagt caatatcgat gaacagcctg
     8221 aagcagtaga ctggttgaaa gcacaaggat tccaaagtgt tcctgtcatt acttctgatg
     8281 cagaaacagt tatcggattc cgtcctgatc agttgcgtaa acttgccagc taacatgact
     8341 aaaagaatca aaacgtgata catggatttg aagggcttga gacagcacca gtttttgtcg
     8401 caaacttcca aatccttttt taaagcttaa aaacaatgca atgatttgca atcaagcatc
     8461 atcaaatacg aaatgaaggt tgggcgtacg tatgagtctt aaaaatttac aagatgtcag
     8521 ttatttcaag ctgaataatg aaatcaatcg gccagtcaat ggtcaaatcc ctttgaataa
     8581 ggataaagaa gctttagttg ccttttttga ggaaaatgtc aaaccaaata cgatgacttt
     8641 tccatctatt atggaaaaaa tcaattactt gatcgatcaa gaatatatag aagcagaatt
     8701 cattcaattg tattcaccat catttattga aaagctatat caatttttgg acgagcaaca
     8761 tttttctttc aaatctttta tggctgctta caaattttat tctcaatatg cgttgaaaac
     8821 caacgacggt tccgcttatc tggaaactta tgaagatcga gtagccttca atgcgcttta
     8881 ctttgcgaac ggggatgaag aattagcact tgctcttgca gacgaaatga tccatcaacg
     8941 atatcagcca gctacacctt ctttcttgaa tgctggacgt aaacgtcggg gcgaattggt
     9001 gtcttgtttc ttgactcaag tcactgacga tatgaactct atcgggcgct caatcaactc
     9061 tgctttgcaa ttgtcccgta ttggtggcgg cgtagggatt actttatcaa atcttcgtga
     9121 agccggcgct cctatcaaag gatatgaggg tgctgcaagt ggtgtcgttc cagtcatgaa
     9181 attattcgaa gatagtttca gctattctaa tcaattaggt cagcgtcaag gagctggcgt
     9241 ggtttatttg aacgttttcc atccagacat tattgctttc ctttcagcaa aaaaagaaaa
     9301 tgcagatgaa aagattcgcg taaaaactct ttctcttggc gtgatcgtac ctgataaatt
     9361 ttatgaattg acacgtaaaa acgaagatat gtatttattt agtccatata gtgtcgaaaa
     9421 agaatatggt gtcccattct cttatgtaaa tattacagct gagtatgatc atctagttgc
     9481 aaatccaaac attcgcaaac aaaaaatcaa agcgcgtgat cttgaaaatg aaatctcaaa
     9541 attacaacaa gaatctggct atccttacgt aatcaatatt gatacagcga accgcgagaa
     9601 tccaatcgat ggcaagatca tcatgagtaa tttatgttca gagattttac aagttcaaac
     9661 gccttctgtg attaacggca aacaggaata tgaagtatta ggaacagata tctcatgtaa
     9721 cttaggctcg acaaatatcg tgaatttgat gcaaagccct aactttggtc aatctgtgcg
     9781 tacgatgact cgtgcattga catttattac agatgcatct gatatcgatg tcgtaccacc
     9841 gatccaaaac ggaaataagc tgaatcatac gattggatta ggtgccatgg gcttacacac
     9901 ttatcttgca aaagaacaaa tggaatatgg ctcagaggat tcattagact ttacaaatat
     9961 ttacttcatg ctgttgaatt attggacact tgttgaaagc aacaatatcg ctcgtgaaag
    10021 aaaacaagtt ttccataatt ttgaaaaatc agcttatgct gacggcactt atttcgaaaa
    10081 atacgtaact ggagaattcc agccaaaatc agaaaaagtc aaagcattgt ttgaaggcat
    10141 ttttatccca actgctgaag attggaatgc tttgaaacaa gcagtaatga aagatggact
    10201 gtatcatcaa aatcgcttgg cagtagcacc aaatggctca atttcatata tcaacgatac
    10261 gagtgcaagt attcatccta tcactcgcat gatcgaagaa cgccaagaga agaaaatcgg
    10321 taaaatttat tacccagcgc cttatctatc caatgagacg attccgtatt acacttctgc
    10381 ttatgatatg gacatgcgta aagtaatcga tgtttatgca acggcacaac aacacgtcga
    10441 tcaaggcatg agtttgactt tattcatgcg ttctgaaatc ccagaaggca tttatgaatg
    10501 gaagacaaca acgaagcaaa cgacacgtga tttgaatatt ttaagacatt acgctttcca
    10561 caaaggaatc aaatccattt attacgtacg tacctttact gatgatgcag aagaaatcgg
    10621 aagcaatcaa tgtgaaagct gcgtgatcta atatcataga tagattcgct tattcatatt
    10681 ttaattaaag gagcattctc atgtcagaaa catattatga agcaataaac tggaatgaaa
    10741 ttgaagatat cattgataaa tccacttggg agaagctgac agagcaattt tggctagata
    10801 cacgtatccc actttcaaat gacctagatg attggcgtac attgtctcaa ttggaaaaag
    10861 atacagtagg acatgtattt ggcggtttga cactgctaga tactgtccaa tctgaaagtg
    10921 gcatggatca gttgcgtaaa gatgtccgca ctccccatga agaagccgtt ttgaacaata
    10981 ttcaattcat ggaatccgtt catgccaaaa gttattcttc tatttttagt acactgaata
    11041 cgaaaaaaga aatcgaagaa atttttgact ggacaaatac gaatccttat ctgcaaaaaa
    11101 aagctgagcg tatcaatgaa atctacaaac acggtacacc gctagaaaag aaaattgcca
    11161 gcgtcttttt agaaacattc ttgttctatt caggttttta tactcctctt tattatttag
    11221 gaaacaacaa attggctaac gtagcagaga tcatcaaact aatcatccga gatgaatctg
    11281 tacatggaac ctacatcggt tacaaattcc aattaggatt caacgagtta cctgaagcag
    11341 aacaagaaac gttaaaagac tggatgtaca atcttcttta tgagctttac gaaaacgaag
    11401 aacgttatac agaagaatta tacgatccaa tcggctggac agaagaagtc aagacattcc
    11461 tacgctataa tgccaacaaa gcattgatga acctagggat ggatccactc tttccagata
    11521 ctgccaatga tgtcaaccca atcgtaatga acgggatttc aacaggcaca agcaaccatg
    11581 acttcttctc acaagtcgga aacggctatt tgttaggtca tgtagaagcg atgaaagacg
    11641 acgattattt gattggattg gaataaggat ttattttaat tcaaaaattt ttcgaaaaat
    11701 aaaaagagtt tagcgtacag atattttaaa tgtacactga actctttttt atgcttattc
    11761 acttgtttct tccagcactt ttggactgtc taacttttcc gaaacatgtt ccattcttct
    11821 caataaatag attttccgga tatttaatat tatcgtctta atcactgcat agacaggtat
    11881 gcaaatcagc atgcctaata ttcctgcgac acttcctgct ccaatcaata ccaagatgat
    11941 cgtcaaagga tggatattca ttgatttccc aaataacaac ggcttgatca aatttccatc
    12001 taattgttgg atcactaaaa tcgacaacgc catatacaaa gcttgaaatg gagagataaa
    12061 caaaccgaca atgattgctg gtaccgctcc aataaaaggg ccgacatacg ggataatatt
    12121 cgtgattccg caaatgaccg caagcaacaa gctataaggc tgatcgaata ttctcattgc
    12181 caaaaagctc attacaccaa taatcaaagc atctaagatc gtactgctga tataggcaga
    12241 taatgtttcg tttaattctt ttaccgtttg gcgtaactct ttacgaatcg ctttcggaaa
    12301 gaagtgagac aacgcatcaa taaatttgtg cccatccttg aacataaata gcaaaataaa
    12361 tggaacggta aataacaaaa taaaaaattg catcataaat gaaatgattt ttgagacact
    12421 tgcagataaa ctgactaata tgatattgaa caaattacta agagataaat ttgctgatgc
    12481 taattgttct tccaaattga aatttttaaa ctcttcccgc tgcgccaatt catttaacca
    12541 tttgcttgtt tcatctgcat acattggcaa gctctgggtc aattgaattg tctgttccat
    12601 taactgaggc actacattca tgacagttaa aacaactaaa atgattacga ccaaaaacgt
    12661 caataaaaaa ccgaacactc tcgggatccg atgcttttcg agaaaaacaa cgattggatc
    12721 gaacatataa tagataaaac cagcaacaag caatggtaaa aaaattgccg aaagcatttt
    12781 caatattgga ctaaaaatat tcggcatttg taaaataaag tacacgccga tgatcgtgag
    12841 tactagctcc actgtccaga aaaataattt tgattgttta aaacgtgatt gcatacatct
    12901 cctccaaggt cctttgatac ttctattcta tcatttaaca aagagttacg cgctgagatt
    12961 ttcatttgct taaaaaaaac aaaaaaaata aacatatttt aatgagtaga cgagtactta
    13021 aacaaaaaca cggtttggag tagtgacaaa aattatactg cagtcccttt ttccaaataa
    13081 acggtgttca aaagcttgca tcttcgtaat aagcccagcc aaacgaaaaa tataaggaac
    13141 tatttttatt tggctgggct tattacttga tgtaggacat ctattttaca acctcagatc
    13201 atttattgcg caatactgac taaatgcaac tttgtgatat ttttgtgtac ggacaactcc
    13261 tcgatcacat cggcataagt gagccctttt ggaagatcaa tcgtatagat atttttgtac
    13321 actcggtagt catcaacgaa ttttacgtca aaattcacat cttcgattac gatatttttc
    13381 tcttcaaaat aatcggtaag aaaatctttg gtttcttttc tatggataaa ttggatttct
    13441 aactttttag tcgtaggtac atggatcacg cgtttcacta ctgtgagtgc aatagcaatc
    13501 ccgataaaac tgatgatcgc aatggaatag taacccatgc cgatggcgat ccctaatcca
    13561 gcaattgccc ataaagaagc ggcagtcgta agtcccgtca ccgattgttt cgtgacgatg
    13621 atcgttcccg cgcctaagaa accgacacca ctcaccactt gagcaatcag ccttgcttca
    13681 tctgaacgaa tcgttccagc aagctgtgga tactctaaag catctcttaa tgcaccacta
    13741 gcgatctcaa cttggatcaa tgcaatgata catgctccca tacaaactaa aatatgcgta
    13801 cgcatacctg ctggacgatt tttatattgt cgttcaaaac cgattgctcc gccaaatagc
    13861 atagcgatcc ctaaacgtaa aataatctct ggaattgtca ataccatatc attcatctaa
    13921 acaccctcca gtccaatata tcatgtctga aaggagcaga caaaaaaagt gcaattttta
    13981 cctcttacga aaaaaggact gtgacaaaat ttttgtcata gtccttttta tgaataaacg
    14041 gtgttcaatc agctgaccct cggtattact caacaaacgg caaaatatgg ggagctattt
    14101 ttcctttgtc ttctctaata ctcaggtcaa aacgctgatt tcacaacctc tttagcctac
    14161 tattgaaaat tacaacttac ttgctgcttc ttcatcgatg atcacaacaa cattgtcgtg
    14221 attttgcaat acacttgctg gcaaatgatt cgttacaggt ccttcaatca tccctttgat
    14281 tgcatcagct ttgctttcgc caaatgccat caagacgatt tctttacttt tcataattga
    14341 accgattccc attgaaacag cttttgttgg tacatcttct gctttatcga agaatcgttt
    14401 gtttgcattg attgttgatt ctgttaagtc aactactgaa gtcgttccgt caaatgaagc
    14461 accaggttca ttgaaaccga tatgtccatt ttgcccgatt cctaagattt ggatatcgat
    14521 tggatgtgca tcgatgattt gatcatactt tttgcatgct gcttccagat cttctgcttt
    14581 tccatctggt acaaacgttt ctttgaatgg ttttttatca aataattgga catccatgaa
    14641 atgacggtag ctttgttcat ctgaaccgcc tagtccaacg tactcatcta agtttacaga
    14701 agtcatttca ctgaaatcca aatcgcttga agtcatttct ttatataaag taactggtgt
    14761 gcttcctgtt gccaatccta atacttttgc acctttgttc ataccttctt tgatgatttc
    14821 aaatgctttt tttccgcctt cttcagcgtt ttttacacga atgatttcca tagtgattgc
    14881 tcctttatat tttggtatag cccaattata gcattctttt tttcttttta caataattag
    14941 tatagaccaa attgcatttt tttcattttc ttgacaataa ataggaatcc gtgttaggtt
    15001 tcataagatg gttcaatcaa acaatccatg ttgcatgaaa accaagcaag tgattcactg
    15061 tggacgcttt atgtctgcag tttttttatg aaaaaggagt tattatgagt atactgacat
    15121 taaacaacat tacccagcaa tttggcgaca aaatcctcta tgaaggtgtc gatctgcaat
    15181 taaatgctgg cgaacatctt gggctgattg gacaaaacgg agccggtaaa agtacattga
    15241 ttaaaatcat cactggtgag atcttaccag atgacggaca gatccattgg caaaaaaata
    15301 ttcacaaagg ttacctcgat caatatgtgg aagtcgatga aacattaaca atcgaagaat
    15361 ttctaaaaac tgcgtacgca aaagaatacg aaaaggaaga aaaaattgct tttctttacc
    15421 aaacatatag cgagaccttc gatgattcct tgctggaaaa agctgggaaa ctccaaacgg
    15481 aattagacca aggagtgttt tatcaaatcg acaccttagt tgctgagatg agcagcgggt
    15541 tggggatcga tgtcttaggt ttagattcac tgttgaaaaa tctaagtggt ggacaacgtt
    15601 caaaggtgat tttagcaaaa ctacttttag aaaacccaga tgttctgatc ttagatgaac
    15661 caaccaatca cttagatgat cagcatatcc aatggctgac gcagtttctt caggattttg
    15721 acggtgcgta tatcgtgatt tcccatgacc aagaatttct tgatcagatc accacccata
    15781 tcgcagacat cgagtttgga aagattacta agtatactgg ccatctaaaa caagcattga
    15841 aacaaaagga acaaaatcgc gaaagctatt tgcgccaata tcatgctcaa caaaaacaaa
    15901 tcgaaaagac cgaagcttat attcgaaaat acaaagccgg ttcccgatca acgatggcaa
    15961 aaagccgaca aaaacaatta gataaaatcg aacggctgac gcctccagct tcagcaagta
    16021 aagctgtctt tgattttcca ttctcgccta ttgtcacgac attagcagtg gaaacaactg
    16081 atttagtcat cggatatgat cgaccgctac ttgcacccat caatttgacc atccgctttg
    16141 gtgaaaatat tgccattcga ggcttcaacg ggattggaaa atctacttta ttaaaaacac
    16201 tgatcggtga gattgaaaaa ataagtggcg atttccactt cccagataac acaaaaatca
    16261 attacttttc acaagatctg tactgggaaa atccattaga aacaccacta caatatttaa
    16321 gcagtcaatt tccaaaagcg actatcaaag aattacgtcg ccaactagct aaagcagggt
    16381 tagttaatca attagcaagt gaacctctca ctacattaag tggtggggaa caaactaagg
    16441 tgaaacttgc acagttgaca atgaaccaag gtaatttact gattctcgat gaaccgacga
    16501 accatatcga tcaagaaacg aaagaaagct tacaagacgg gatacagaaa tttccaggaa
    16561 cagtaatcat cgtctctcat gaacaagaat tttatcaaga tcttgtgcaa cgagtcattg
    16621 agattgaacc aaaataaacg tttgattgaa tagttgaaaa aaaggagctg tgacaaaagt
    16681 tttgtcacaa ctccttcttc tgaataaatg gtggccaatt agctgacctt cggtattagc
    16741 tcaaaaaacg gcaaaatatg aggagctatt tttatttggc tattcttatt acttgatgca
    16801 gaacagcttt tcacaacctc ttttcgtcta ttttttattt tcgagatttc tctttccttt
    16861 aaaaacaact gtttgaaaga agaacgcaca tccttgaaaa acaagcccgc taaccgttac
    16921 acctaaccag ccatactgct gccacattgt cgtccctagc aatgaaccag ccgcacctcc
    16981 aataaagtac ataaacataa ataca
//
LOCUS       DACKAC010000060        16995 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.061, whole genome shotgun sequence.
ACCESSION   DACKAC010000060 DACKAC010000000
VERSION     DACKAC010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.061"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..1152
                     /locus_tag="I0B46_002075"
     CDS             <1..1152
                     /locus_tag="I0B46_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084349.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="I0B46_002076"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="I0B46_002076"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAR2084350.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="I0B46_002077"
     CDS             2728..3003
                     /locus_tag="I0B46_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAR2084351.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="I0B46_002078"
     CDS             3009..3776
                     /locus_tag="I0B46_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAR2084352.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="I0B46_002079"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="I0B46_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAR2084353.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="I0B46_002080"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="I0B46_002080"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAR2084354.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7920..8222
                     /locus_tag="I0B46_002081"
     CDS             7920..8222
                     /locus_tag="I0B46_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAR2084355.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8225..9388
                     /locus_tag="I0B46_002082"
     CDS             8225..9388
                     /locus_tag="I0B46_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAR2084356.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9414..12842
                     /locus_tag="I0B46_002083"
     CDS             9414..12842
                     /locus_tag="I0B46_002083"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAR2084357.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12863..13987
                     /locus_tag="I0B46_002084"
     CDS             12863..13987
                     /locus_tag="I0B46_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084358.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13977..14414
                     /locus_tag="I0B46_002085"
     CDS             13977..14414
                     /locus_tag="I0B46_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAR2084359.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14398..14736
                     /locus_tag="I0B46_002086"
     CDS             14398..14736
                     /locus_tag="I0B46_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAR2084360.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14855..15415
                     /gene="rsmD"
                     /locus_tag="I0B46_002087"
     CDS             14855..15415
                     /gene="rsmD"
                     /locus_tag="I0B46_002087"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAR2084361.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15412..15903
                     /gene="coaD"
                     /locus_tag="I0B46_002088"
     CDS             15412..15903
                     /gene="coaD"
                     /locus_tag="I0B46_002088"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAR2084362.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15893..16936
                     /locus_tag="I0B46_002089"
     CDS             15893..16936
                     /locus_tag="I0B46_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAR2084363.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
BASE COUNT         5752 a         2945 c         3750 g         4548 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggctatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
     7861 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
     7921 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
     7981 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
     8041 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
     8101 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
     8161 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
     8221 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
     8281 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
     8341 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag
     8401 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
     8461 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
     8521 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
     8581 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
     8641 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
     8701 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
     8761 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
     8821 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
     8881 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
     8941 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
     9001 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
     9061 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
     9121 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
     9181 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
     9241 tggaggaatt actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg
     9301 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
     9361 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
     9421 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
     9481 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
     9541 aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata
     9601 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
     9661 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
     9721 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
     9781 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
     9841 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
     9901 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
     9961 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    10021 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    10081 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    10141 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    10201 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    10261 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    10321 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    10381 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    10441 tcactacaga agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc
    10501 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    10561 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    10621 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    10681 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    10741 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    10801 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    10861 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    10921 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    10981 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    11041 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    11101 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    11161 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    11221 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    11281 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    11341 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    11401 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    11461 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    11521 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    11581 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    11641 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    11701 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    11761 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    11821 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    11881 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    11941 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    12001 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    12061 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    12121 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    12181 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    12241 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    12301 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    12361 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    12421 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    12481 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    12541 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    12601 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    12661 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    12721 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    12781 atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat
    12841 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    12901 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    12961 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    13021 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    13081 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    13141 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    13201 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    13261 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    13321 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    13381 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    13441 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    13501 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    13561 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    13621 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    13681 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    13741 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    13801 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    13861 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    13921 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    13981 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    14041 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    14101 aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg
    14161 catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa
    14221 gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa
    14281 gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag
    14341 atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg
    14401 caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac
    14461 agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga
    14521 atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc
    14581 cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat
    14641 tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag
    14701 ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa
    14761 ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat
    14821 actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg
    14881 cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa
    14941 agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt
    15001 tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg
    15061 cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga
    15121 accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc
    15181 ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat
    15241 cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg
    15301 cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa
    15361 acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt
    15421 gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt
    15481 gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt
    15541 ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat
    15601 gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca
    15661 gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct
    15721 cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa
    15781 tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc
    15841 agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc
    15901 taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt
    15961 agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact
    16021 aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg
    16081 tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt
    16141 gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca
    16201 gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa
    16261 gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt
    16321 ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag
    16381 tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag
    16441 ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga
    16501 taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga
    16561 tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga
    16621 aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg
    16681 aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag
    16741 tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt
    16801 taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa
    16861 attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttgactatct
    16921 agagaaaatg aaatgaaact aaataattaa agaggggttg tgacacgagt tctgtcacaa
    16981 cccctctttt tcgtc
//
LOCUS       DACKAC010000061        16740 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.062, whole genome shotgun sequence.
ACCESSION   DACKAC010000061 DACKAC010000000
VERSION     DACKAC010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.062"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(99..1229)
                     /locus_tag="I0B46_002090"
     CDS             complement(99..1229)
                     /locus_tag="I0B46_002090"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAR2084364.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDRLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1878..3080)
                     /locus_tag="I0B46_002091"
     CDS             complement(1878..3080)
                     /locus_tag="I0B46_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAR2084365.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="I0B46_002092"
     CDS             complement(3140..3343)
                     /locus_tag="I0B46_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAR2084366.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="I0B46_002093"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="I0B46_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4880..5692
                     /locus_tag="I0B46_002094"
     CDS             4880..5692
                     /locus_tag="I0B46_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAR2084367.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6716..6868)
                     /locus_tag="I0B46_002095"
     CDS             complement(6716..6868)
                     /locus_tag="I0B46_002095"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084368.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
     gene            complement(7059..7937)
                     /locus_tag="I0B46_002096"
     CDS             complement(7059..7937)
                     /locus_tag="I0B46_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAR2084369.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(7976..9094)
                     /locus_tag="I0B46_002097"
     CDS             complement(7976..9094)
                     /locus_tag="I0B46_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAR2084370.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(9091..11190)
                     /locus_tag="I0B46_002098"
     CDS             complement(9091..11190)
                     /locus_tag="I0B46_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAR2084371.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(11241..12062)
                     /locus_tag="I0B46_002099"
     CDS             complement(11241..12062)
                     /locus_tag="I0B46_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAR2084372.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(12059..12868)
                     /locus_tag="I0B46_002100"
     CDS             complement(12059..12868)
                     /locus_tag="I0B46_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAR2084373.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(12889..13383)
                     /locus_tag="I0B46_002101"
     CDS             complement(12889..13383)
                     /locus_tag="I0B46_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAR2084374.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(13395..13883)
                     /locus_tag="I0B46_002102"
     CDS             complement(13395..13883)
                     /locus_tag="I0B46_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAR2084375.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(13960..16656)
                     /locus_tag="I0B46_002103"
     CDS             complement(13960..16656)
                     /locus_tag="I0B46_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAR2084376.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
BASE COUNT         5091 a         3609 c         2693 g         5347 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ctatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt
     6421 tcttattttt cactaaaata aaaaatttat tgcaaaaaaa atagaagtga caaaattatc
     6481 aatcatttca aggtaatata actacctgag tttattatcg attaaaatgc taactacatt
     6541 tgcaagagaa gcgagagtta aaggtaaata tagaatttct acttttttag caatagaaat
     6601 aggaataaaa tatttaaaat gtatagaaac tttctttttt tttcttgcta tctttttata
     6661 atcacaactc tctataatag aattacgatt tattttatac ggctgaaaaa atctatcacc
     6721 aaaaaaattc aaacattaag ccaaggatta tgaagtcaac atttcgaaaa atgaacatac
     6781 attggtaagc aactataact attaccacag acataaagta acacaatata ttactattta
     6841 agtgtacgcc accagtgaat atcctcaagg tatacagcgc acaacaaaaa gaaaaaagga
     6901 aacgcagaag catttccttt cagtgattta atgaaaacaa cacgatttga cttagagcct
     6961 ttttatttga attcattttc gctttatcca acttcatttg atcatttgat tactcggtta
     7021 aatacttttg acatgagtat caatgaaaca ttcattaatt atggttgctt atttcttacc
     7081 gcttttctgg ccaatgctaa tccgccgatt attccggcat tatcacctaa ttcgggcgta
     7141 ataatgtagc tatcaattgg tggtatttca acatatccat ttataagctc aacaaattta
     7201 tcctgaactt ttttcttcat gtgttcttgt ttcattacac caccgccaaa aataattcta
     7261 tctggcgaaa acatcagtgt tgtattgtat gcacattggg caaggtaaaa ggcttcaatc
     7321 tgccaaaacg aatcgtcagc caataaattc tggcctttta cacccaaccg tttttcaatc
     7381 gctggtcctg ctgccatccc ctctaaacaa tcttggtgga agggacagtt tcctgaaaaa
     7441 ttgtcctttg gatgtcgctt gatcaacata tgccccattt ccggatgact gaacccttcc
     7501 acgaaagtcc cattgaacaa ggcaccacct ccgatacctg ttcccacagt gaagtatgca
     7561 acacctgcta accctttgcc agaaccagca acatattcac catagcaagc cgcattgaca
     7621 tctgttgtcc attcaatcgg aatattgaaa tattgcttta gcgttcctac aaaattgaaa
     7681 ttttgccaag caagttttgg tgttgatgtg atatgaccga aactcgttga tttgcattga
     7741 atatcaattg gaccaaagca gccgaccccg atgcctgcca attgttcctt aaaaggttta
     7801 aaaaaatcaa tgacctgctt cattgtttca actggagttg ttgtgggaaa actgactctt
     7861 tccaaaatcg ttaattcctc agtaccgact gcacagacaa atttagtccc tccagcttca
     7921 attgccccat atatcatcat aggttcctcc cttcgaatat gtgaaaaaca cttcattaac
     7981 gcggcaagcg ttctcgatca gtgaaatgac gtttagtatt tcctgaagct tctgttgaat
     8041 accagtaagc gacactagca acatcgtctt gccgctcaaa taagcgaaca tcatcgttgc
     8101 cgatttgttg taaagtgacc gataaatctt cggtaaaaca gatggggtct tgaagatgcc
     8161 aacgataaag accatgtcgt ggcattgcgt cgtcaccaaa ggcatgaacg gcattcaatt
     8221 ttcccgtttc aaaacgatct cttgtggcat ctctatttga ttggtaagga taccctaaaa
     8281 aggctgttga atagtttttc gcatgtggac gtccgaactc atcgtactca tgaaaggccc
     8341 acgccccgcc aaaataatct tctgaacctg ttgaggttac ggtcggatac tcactatccc
     8401 catcaatgta gaatttaaac tcgccttcgc cccaccaata acgttctagg caggtaagcg
     8461 caaggtaagt acccacgtaa tagccttgac cctttaatcg atcaattaac ttataatcct
     8521 cctgtaattt ggtaatgcgt tcccgatgcc aatttgcgtg aaaatatagg ctattttctt
     8581 ccaattcgtc caccaaagcg taatttatcg tataaaaaaa tgaggacata tccttcggat
     8641 gtaaattctc gatcgtaatt ttagcggatg tttggaaagg catttcgaaa taggaattaa
     8701 agccccctgt aggattaacc actatcggca acgagttcac ctcgcaacgc tctccaaacc
     8761 cattacaaaa gaaatcccct aaaggacttt caacagcagg gctatccgac ccatcccaat
     8821 aaattcttaa gattaaatca cgtaaaacaa aactgccaaa ttctgttcga tcacgaatcg
     8881 tcatccacat atgacgaatt attcccgttc cagtaatatc cgcaatcaca gtttcttcac
     8941 cagaagtcag cggaattgcg ccacgacctt tgcggcttgg tcctagctcg cttggcaaca
     9001 tcgcagcttt acctttctcg cctgttggat tttcgggagt gattgctcga ctctggattt
     9061 ttttaaattg ggtaatattt tttagtatac tcatcttttt ctcctttgaa tgtgaatcgt
     9121 ttttcccttt tctacgtgaa gaattttttt cgtatcactt attttcaatg gcttttctga
     9181 caaatttgaa atactcaact cggattgcgt acattcaatc gaaatgaagg cttgaccgaa
     9241 cttcaaattc ttccaagaaa acgttaaatt atcaacagga tcaatttgca atactccttt
     9301 tttgaatttc aatccgaaat attgactgat cattaggctg acaaaaagac ccgatgccca
     9361 gccgcatttg ccgcagccaa agtctcgcac gacttctcct tgttgagcct ctaagcgata
     9421 cggccaccac caccacgaac cgtctaaatc tgccaaacaa attagttctt ctaatcgggt
     9481 tacgaaagct tgagggttgt gcagctcgct cattaaaaca gtgataaagc caggaaaagt
     9541 ggcgccggat tctaatcccc aagtgatgcc tttgatttcc tcgctatagg ttggattgaa
     9601 agaactagca gaaaaggcca tggtgttctg atataaatga tctttattat ctaaaaattg
     9661 ataaaaaggc atcaacgtag tgtctgattc ttctccatca tgcatcaata aattaatttt
     9721 tccatccaca atcacatcac ttaaaaagat taacccttgc tcaagaatcg gtttttgata
     9781 gtgcttcact gaataggttg ttttctcctc atcaccgatt ccctccaaaa attgctcacc
     9841 aaaaggacca tcgatagtca tttgtttaca aattgattcc tttagtttac aggcaatgtt
     9901 tttgtatcgc ttgctaagac tcagttgttc tagggcttca aaaatttctg caagtccaat
     9961 acaggccttc caaagacaaa gattgctgcc ggtatgatac tttccaagtg caaaggcgtc
    10021 tgataaccaa acagatggaa aaagcattgg ttcccctgac cgacgttgag ctaatatgtc
    10081 ctcaataatt cttgaagcat tcagtagaac ctgcggacac cgctcaaaga attttgtatc
    10141 cccagtatgt tcaaaataaa gactagccag taacgcactg gataacgaat tactcagaga
    10201 atgattgata cctcctgtaa attttgaacc aggaaactta acgccatatt gatcaaacca
    10261 tagaaccgtt ttttgacata actccggttc aaacatggtg aatggcaatg atgcgtaata
    10321 catatcctta ttccaaattc gattagtagc aggatagctg ccccaattgc tgccgacgac
    10381 ctgtccctgt cgattcatac cgaaactaga aaagctttga taaagtgcac gacgatagag
    10441 atggacaact ctatcgtttg gcattgacag ctgcccaaat agctgagcaa aatagttcaa
    10501 tgtttccttc agccattttt cgatatcaga ttcctgaaaa gtcattactt cttcaaaagc
    10561 atttgggtcc acaaaagcaa tggcgaagct tgccacttcc tgttgtgcga tcgtgtcctt
    10621 atttcctttt tgagaaatca acacattttg ctgatccgaa tacttctctt gatacgaagg
    10681 cacatctttc actgaaactt gaaggctttc gtgtgtttca ttcacaatgt aaacctgttg
    10741 aacaagcata cttaaacgct tattagccag tatgggagca aaggatacta caatgacctt
    10801 aaacgtcgaa taatgatgaa tatatctagg caaacagtct tgaatcacat cgacttcaat
    10861 acgatgatcc gattctgaca aatggtagac aatcccgttt acttcaacct ttaaggataa
    10921 cgaattggtt tgagagagct gtttattgac ccaaacacct ggtttttcct cagttagtaa
    10981 attttctcga taaaaggtca acgtttgaat cgtcccgcaa acatcaaggt ttgctaatgt
    11041 cttttgatta cttacatcga aggtcaatcc ctttacatca ggaatattcc gatagtagct
    11101 gtattcgttt gaagtagtca catttccgtc gtttaactca gcattaaaaa atgggtgttt
    11161 gggtgaataa attacttcat taatagtcat cttttccctc cttgctaaaa aaactgcagc
    11221 ctagtagctg cagcttccta ctatgcgatt ccaagtaatg acaataaaat tcccaagacg
    11281 accattccaa aaataatttt gatgacgccg acttttttat ctagtaattt gaaacagctc
    11341 aatgttacca ataacggtac aattccttta aaaatactat cgaggatttc ttggacattc
    11401 attagttctt cgcccttcat tgaaaagctc aaaacggtat tgaatttgac catactgatc
    11461 gtcattgcac ctaccatgat cagacccaag atactagcag cttttgtcag aatttcaatt
    11521 aacccgttcg catacagctt ttcaatatat ttagagccta atgaatagcc taaaacgccg
    11581 ccatagtatc gcatcaagta attaggtata ttgaacataa ctaaaaagat aattggtgcc
    11641 aaaatatttc cggcattggc taagttgatc gctagtcccg cggtaacgac acgccaaact
    11701 ccccagaaca atgaatcgcc aattccagcc aatggtccca tcaaactcgt tttgatcgcg
    11761 ttgatacttt ccacgtcaaa accagctttt tcagaatttt ccttctccat cgatgccgca
    11821 atccccatga caaaggacgc gcaaggaatg gtcgtcgaaa aatactgtgt atgacgttcc
    11881 agcgccgcag ctcgatcttc ttcacttttg tagaaatgat taatgaaggg catcatagaa
    11941 taggcgaaac caccgccgcc catcgtcacc ggattcaccg aagcatacat agtcatcgag
    12001 cgccaaaaca tcttccgaac caattttttc tcatctttag aaattccatc agctaatttc
    12061 attgaaagaa atcctcctct tcttggtcaa gcgttgcatt tacagcagca tcgctttgaa
    12121 ctgcatgaac agatatattt tttagttgta gatctctttg ggctataaca attgcaataa
    12181 tcgttccaac tgcggcaatt gcaatcaatg gtaactcgag atacgccata acaacggccc
    12241 caaagaaaaa ccacacagca atatttttac tccaaagcat ccgtaacagc atcgccatcc
    12301 caactgccgg cagcagattt ccagcgactg ttaatccgtt catgatgacc tctggaatat
    12361 tatccaagac gcttttgaca acatctgctc caaataaaac ggcaaagaat ggaataagag
    12421 cataaagcgc atagtggaca aaccacataa agaagtgcag attgatgatt cctttttgat
    12481 caccagattt tgcaaatttg aaaaatttag cagccatcgg tccacataca aaagaaaaaa
    12541 tcatattcat taactgtaat cctaatactg ctagtggaaa gacaatggga ataatcgcat
    12601 ccacgtttcc tcccattttg attgcgaacg ctgtagccag cccagcagcc aaagaaggct
    12661 ccgccgtact agagattccg atattgacga cacccataaa gatggcttct agtgacgccc
    12721 cgacaatcaa ccccgtctgc aaatccccca tcaacgctcc tgctaacgga gcaacaacaa
    12781 tcggacgact cagcatggac atcccgaaca tttcatgtcc agcaactcca ataaaaacgg
    12841 ccaaagcgac tacaaatgca tcatacataa tttttctcct tcctttttct aaaaatcact
    12901 caaaatagag atagctgaac gtttttccat cgccggtgta ggctgaaaaa cggtatctgg
    12961 ataaagttca actaattctt ttaagtcttg aatttctgac gtcgttaaca tcacttcctt
    13021 tgataaggtt ttcttatctt ccgatggcgg tgtcattttc ccagcattcc ccacgttcat
    13081 taattcgatt tccgttggga aagctttcgc aacttttacc gcatctttga cattagggac
    13141 taaaaccatc aagcgcatgt ccttcgcccg cggatctttt aacaaatgaa tcgcgtcatc
    13201 aactgtttta ataatgactt tgacattcga tggtgcagcc atcttcaatg tcatcttttg
    13261 aatatcgttg gtagccgcct catcgcttgc tactactaac ccggttaaat ttagactctt
    13321 tgtccaagtc atcgctactt gtccatgaat taatcgttca tcaattcgca ctgctttaat
    13381 catttttctt cctcctaaaa aaaatcttcc tcttcattta ctacatgtac ctcatcaaga
    13441 atggtcatct ttaattcctg ttggcattta tttaccagtt cctgaatatc ctcctttgtt
    13501 aattccttgg ttgccaatac tatttcgagc agaattggca gattaaagcc agacaaaata
    13561 gttacagcaa tctctttctc caaaaaggtt ttggtaatct tctgattgac acttccgcca
    13621 aataaatcgg taaaaacaaa aatctgatcc tgctcgttca cctggtttac aaatacattc
    13681 aactcctgtt caaaatcaca ctcatccaca taagcattaa tcaccgtaat ctcttgaacc
    13741 ttccccgtca gtaattgaat actgctaaga aagcctgaag ctaagtgacc gtgagtcgct
    13801 attagaattt tcttcatatg gttaattcct ttctttctgt tcacttttat ataagcaatt
    13861 cctgtgccaa cttttaaaaa cattggttta atcacgttaa ttcacattaa aaaaagtgtc
    13921 taacctaaaa aaaacagatt agacactttt ttagacactt tatttttgaa tatacggtcc
    13981 taataattca tagagcagcg aaagctcata agcattgact ttaaaattat atttcatctc
    14041 cattgattga aaaatacttc tagaaacata ataaaattca tcttcatctg gtttcaaatc
    14101 ataattcggt tcttcagatt tttctgtccg agccaagaat aaacgttcca tcatcaatgc
    14161 cgtatgcata tagagattta gttttacacg gccatttaaa gtgaaatgat agtaattttc
    14221 atatttagaa atgactgttt ccacttcact catcacgatc gttgggttca gaaagttcaa
    14281 acggtccgca attccttcca acgaaaagaa ttttactagc tcctgaatca agcggttaaa
    14341 ttctgccggc ttcatttcag gactcaaaat atgctccaaa ttggctgctc catcaccgtc
    14401 taagatgtca taaacattaa tattaggaat tgaaaaggaa cgtggaagat ccgaagtcgt
    14461 gatgaccaat tttgtcttgt caaaataagt ttcatcattt tggtcgatta attcacgtag
    14521 ttctctgtag tccttcgtga aaacttcgat ggattcatcc gataaatgat gacgaaaaac
    14581 atctttcaat ttgtccgaaa tccccaaacc cgacatacag gaaatgataa tgttgctact
    14641 ttgagaaaag ccttcaaagt attgaacatt gattgaatac ttgtctgctg catcctccgc
    14701 aatctgttta aatggcgcat tcatcgtcat cttcaagcca atatcaagtg caatggaagt
    14761 cgtcaaatta ttcagaagaa gtaactcccc ttttaaatcg ttcttgatct gtgtgtaaat
    14821 ttgatttaaa gagcccatat ctacgatcag cactagacta tctgtattca cttgccgttt
    14881 cacgaaattc cttgtcgatt cgacaatctc tgacacatta ctctctactg gcatatccaa
    14941 cgcttcaaaa acataattgc cgcataattg attcactact gcttgaatgc tgctagccgt
    15001 actatctccg tgagcaagca acaggccctt cagctcgatg gattcatcta cataatcaga
    15061 taaagtcacg acaataagca gtcttagaaa gttctcgtcc ttttctgaga gcggtggtaa
    15121 atgctccaag aactgattac ataagtaagt cgtccgaggt agatttgcag ctaatgtacg
    15181 atttatcccg gttaagtctg tcttatagct tggggaaatg acataggatc tgctaataat
    15241 ttctgcaaca ggtccactcg tcgtcaatcc gtacgttttt cctaaaacgg atacccagac
    15301 ctttctaaac atctgaatca tcaattttct actcattgat aaaaaagaat ccttatcaat
    15361 gtgatgcacc aaatgctgta cttcattctt tatcgaagaa aaattttcat tgaaattacc
    15421 ttcatttatc agctgagtaa ttttttccaa aatcgaatca taatcaaacg gtttgattcg
    15481 atccgttttt atcttttgat ttttctttat caataaacta gcaatcggca agctttctac
    15541 ttcggattcc agcatattta actgaatcaa gatctcattt ttcccaatcg ttaatggttc
    15601 ttcttgtgtt cgactataca catccgcaca actgatttta atgatattct tcagcttacc
    15661 aatattccca ctaaagggat gagataacat taaagacaag gcttctcttg aaacaataat
    15721 atctttttgt aaaacatccg cttcattttg aaaaaataat tgaatcagct ctaatcgttc
    15781 tcgaatgggg cgatcttcaa aagttggcaa cataacacta atttgaatac gccgatcaaa
    15841 ggtgtctaat aaataatttt cacctttttc cgtcgtggcg aaaataaaac gaaccttagc
    15901 agaatgccaa tttacatttt caccgactgg acgatagttt ccagtatcaa taaatagaaa
    15961 gagcttttct tgattttctt tcgataggcg atggacctca tcgagaaaaa gatagccgtt
    16021 gttggccgtt gccaataaac catcggtatc cttttcagcc cccgtaaagg cccccttctt
    16081 gtatccaaac agtgttgccg aaagcagttc gggattgtcc gcataatcgg cacagtttaa
    16141 gatagcaaat ggtgcatctt tttcaataat ttgctcatgg atagcgtatt caaaaatttt
    16201 tgtggctaaa aaactttttc caacacccgt ggctccattt attaatacac tgagtccgtt
    16261 aggcggatat ttgacagcgg ctatacattt ttgaatgacc tctctcagac ttccgtcatg
    16321 accaactaca gaatgaaagg cttcgtttat ttgctccgta ttatctacaa ccgcatattc
    16381 atctaaaggc ttccacaaaa caggctttcc cggtaatttc tcgacctttt tatcttccac
    16441 taatctcgtc aaataatggc tggcattctg acgagagata cctagattct tcgaaatttt
    16501 ctcggcagtg acagaagaca tattccctgt tagtcgtatc agctcatcaa aaactcgatc
    16561 tttagcgctc tttttcattt tttcctccag tttaatgtgt ctaaaagcat aataaactat
    16621 cggctgcaat atgtctattc tatatttgta ttccaatttt tactttgaaa cacaaatagt
    16681 cgaatatttg cctcagatat ctgacaaaat aatttatcta ccaatttagt ttttatctaa
//
LOCUS       DACKAC010000062        16594 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.063, whole genome shotgun sequence.
ACCESSION   DACKAC010000062 DACKAC010000000
VERSION     DACKAC010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.063"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(45..779)
                     /locus_tag="I0B46_002104"
     CDS             complement(45..779)
                     /locus_tag="I0B46_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAR2084377.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRFVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1105..1515
                     /locus_tag="I0B46_002105"
     CDS             1105..1515
                     /locus_tag="I0B46_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAR2084378.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     EGKTAEGVEALLGYVRKKKAGRVSVNEMEENQID"
     gene            1508..2209
                     /locus_tag="I0B46_002106"
     CDS             1508..2209
                     /locus_tag="I0B46_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAR2084379.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2209..3822
                     /locus_tag="I0B46_002107"
     CDS             2209..3822
                     /locus_tag="I0B46_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAR2084380.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKKTIKTITKKEKKDDI"
     gene            3812..4033
                     /locus_tag="I0B46_002108"
     CDS             3812..4033
                     /locus_tag="I0B46_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAR2084381.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4030..4791
                     /locus_tag="I0B46_002109"
     CDS             4030..4791
                     /locus_tag="I0B46_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAR2084382.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEELSLRFPETFAYALGDVRQMKELQQAVSKCQDRFGEVTILLNTSGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRRKSGTIINMASIAGLVAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5172..5681
                     /locus_tag="I0B46_002110"
     CDS             5172..5681
                     /locus_tag="I0B46_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAR2084383.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANMASPLGLIDVVVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5751..6290)
                     /locus_tag="I0B46_002111"
     CDS             complement(5751..6290)
                     /locus_tag="I0B46_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAR2084384.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVVVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6430..7041)
                     /gene="rpsD"
                     /locus_tag="I0B46_002112"
     CDS             complement(6430..7041)
                     /gene="rpsD"
                     /locus_tag="I0B46_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAR2084385.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7357..8154
                     /locus_tag="I0B46_002113"
     CDS             7357..8154
                     /locus_tag="I0B46_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAR2084386.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPAATILV
                     EGMFANIVVNTAVLISMRMKEDAGKVITVVFIIFIFAFLGYEHVIANFPAFTLAYFAS
                     QGQTDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            8182..8817
                     /locus_tag="I0B46_002114"
     CDS             8182..8817
                     /locus_tag="I0B46_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAR2084387.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVYYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8837..9610
                     /locus_tag="I0B46_002115"
     CDS             8837..9610
                     /locus_tag="I0B46_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAR2084388.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10018..10815
                     /locus_tag="I0B46_002116"
     CDS             10018..10815
                     /locus_tag="I0B46_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAR2084389.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKKIQLIQINDDRPFME"
     gene            10793..11206
                     /locus_tag="I0B46_002117"
     CDS             10793..11206
                     /locus_tag="I0B46_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084390.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEQEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11190..13835
                     /gene="mutS"
                     /locus_tag="I0B46_002118"
     CDS             11190..13835
                     /gene="mutS"
                     /locus_tag="I0B46_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAR2084391.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLKKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKNSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13853..15961
                     /gene="mutL"
                     /locus_tag="I0B46_002119"
     CDS             13853..15961
                     /gene="mutL"
                     /locus_tag="I0B46_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAR2084392.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEEPKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPVV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15983..16543
                     /locus_tag="I0B46_002120"
     CDS             15983..16543
                     /locus_tag="I0B46_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAR2084393.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARIVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
BASE COUNT         5387 a         2964 c         3599 g         4644 t
ORIGIN      
        1 ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc cataaataat
       61 aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac tggtacccat
      121 gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg tacaaaacgt
      181 ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag tgaagcaact
      241 aatgtccata caaggaaacc gagtgctaag tgagctgtac caagattatc ttggaagaat
      301 ggtgcttttg taatacctaa ggcaccaaag aataaaacga atgaaccaaa gaattcattg
      361 acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc taacacatgg
      421 ttcacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat caattgtcca
      481 atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca agggaataga
      541 ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct gattccacca
      601 aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag catccagtca
      661 ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt acctagaacg
      721 attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga gtaatccata
      781 aatacctcca aaagttataa aaatgcaaaa aaaaagaaac atcaacacga aaagcatcgg
      841 tgcagtgttt cgtgtcggaa ggatttctgc agtcttaaca aatgtgaata aaaaaatgaa
      901 ttccattact tataagaaac cagaccaaca gtaaaactcc aactcttttt aaaagttctc
      961 cacaaaccac caagcttaac tataaatata ccaaaaaaaa aacgtttttt ttctcaaaaa
     1021 ttgtatctta gaaacagcat gttttgaaac tttcagaaaa ggtttgttag taaaatgaaa
     1081 gctatacgta aggagtgttg tttgatgaaa ttttttcaag agtttttcat tattattttc
     1141 ttttctgtat taggagaagg catacggatt tttgctcatt tacctattcc tggaagtatc
     1201 ttaggtattc tttttttatt tttagcattt gaaatgcatc ttgttgatcc tgaaaaaatc
     1261 ggagcaactg gtgatttttt attgaataat ctagctattt tatttgttcc tgctggtgta
     1321 ggtttattgg aatattttga tgatattgct tcgatatggc ctgttttact gggagcagtc
     1381 gtcgtgtgtt ccatcgtcac gatggttgca gagggaaaaa cggcagaagg tgtagaagct
     1441 ttgctgggct atgtccgtaa gaaaaaagca ggaagggtat ctgtaaacga aatggaggaa
     1501 aatcaaattg actgagtttt ttgtgaatcc actatttggc ttaagtttga ctttaggagt
     1561 gtatgtatta tctgataaat ttttgcggaa atataaactg cctgttttga atcctctcgt
     1621 attatcgatt gttgttatta tcttattttt gcatttttct gggatcagct ataaagacta
     1681 taatatcggg ggcaattttt tgactatgct gattactcct gcgacagtgg cacttgccat
     1741 ccctctttat aaaaacttcc gtttactgaa agagaatttt tatcctgtaa tggctgcgat
     1801 tttagcagga atagtcgcaa attgcttggt tagtgtaggg ttcggttact tcttctctct
     1861 tcataaagag atggtgatct cattactacc taaatctgta acgacggcta tttctgtcga
     1921 tttatctcat acgatgggcg gggtgaatgc cgtgacactt gctattgttg tctctacagg
     1981 tatttttggt tcgctgatcg ccactcatat tttccgccta ttcaagatcg aaagtccagt
     2041 tgctcgcggt gtggcgttag gaagtacttc tcatgctatc ggtacagcaa aagcaatcga
     2101 gattggcgaa atagaaggaa tcatttccgg acttgcgata tgcgtaaatg ggatattgac
     2161 cgttttgttg ctgccgttat tctttcagcc ttttgctggc ttgttttgat gcgaaaacca
     2221 ctattttatg ctacaataac caaaacgttc gagcataaaa aaggagggaa agacgtgtat
     2281 ttgtcttccg agtatcttaa acaatttgat aaagatctat attataagat tctcctccta
     2341 gaaagttttg aggatcaagc ttggcatacc gctgcacaat tagcgcaagt tgttcagctg
     2401 gatgcccgca gcgtgtcgaa atacctgaac gagctgtcta aaaactatca gcagtttagc
     2461 ggtaagacac accctttgtt tacaaaaaat caccgttctg gttataactt ttatgatacg
     2521 ctcgattcaa ttgaacatga gcgttttttg atctatctcg tgcaaagtac gctgaaattc
     2581 cagttgctgc acgacatttt ttttgaagaa ttccatacaa tgtaccaatt tgcgcaaaag
     2641 cattacatta gtgaatctac tgctcatcga aaaatcaatg aatggaaaca gcaactgcaa
     2701 acttacggaa ttcggctgca acgaggtaca tatatcgctc aaggcgaaga agaaatcatt
     2761 cgactttact tgcatatgac attctggcag ttgtttcgag ggaaaatctg gccatttgaa
     2821 acgatttctc aaatggacgt aaagaatatg gcagaacata tcatggcttt ttttaatgtt
     2881 cgtctgaacg agatcaaaaa aagacgattg gaatacatgc taggtgcctt ttttttaaga
     2941 aaatcacaga agcattatgt tgtattgaat gagaaaaaaa gacggctgat ttctgacaat
     3001 ctgttgttcc agcgattttg ccaagtcatg gagccggtat ttcctaatta ttttcaagta
     3061 gaagacgagt taggcgcatt atttcttgta ctgatgactc gtgaagaata ttatagtgat
     3121 cctaagatac ggaagaaaat ttttgatttt catcaacaag caaaaacacc gccctttaca
     3181 gccctttcag aagcaaaagc cgctctttct ctttatcagg aagagcaggg gctgccagca
     3241 gaaaatctaa ccttcgaggc agaaaactat ttattcagca gtcatttttt cgcctatctt
     3301 tttcctaatg cgaaagaaac gattgatggc aatagtagtg attttattaa tcatttagtt
     3361 attgaaaaca aggaattgaa acaatggctc gtacattttt ttgaaagtag acacaaacat
     3421 cccaatcatc tggcatttaa aaatcatgcg tttttgatgg gtcggtatct gactgtgttt
     3481 aaaacattag gagcctttac tccacagctt ccaaagatca cgatcctttt aatgacagat
     3541 ttcccattat ttgaagagca actgttagag gagggattac ggacattttt ccgtaatgag
     3601 tatcagctga tttttctgcc aacagattat cgggggagag aagttgatct tctgatttcg
     3661 acaagtaaag tccaccgcaa accatgggcg gatcttgact attttattgt gaccgaagaa
     3721 ctaaaattaa ttgattacat ccaactatct caaaaatttg aaatgattca aaaaaaaact
     3781 attaaaacaa taacaaagaa ggagaaaaaa gatgacattt gaagaaattt taccagaatt
     3841 gaaaaaaggt gcaaagatta ttcgtaaagg atggagtgga tttgaacttt atgttgttct
     3901 cgtatccgat gatgaatttg atggctgtcc agtgacacct tacttattga tcaaaacaag
     3961 tgacgaaggt ttttccagct tcgctcctac agtttgtgat attttagcag atgattgggc
     4021 gatcgtagaa tgaaaaacca gtatccagat ctagctaaac aggttgtatt tatcacagga
     4081 gctgcttctg gaattggtgc tgcgcaagcc cgagcgtttc ttgaacaaga tgcttttgta
     4141 tttggattag atcaaaagtt cggtaaaatg gaagaactct ctttgcgatt tcctgaaact
     4201 tttgcctacg cactaggtga tgttcgacaa atgaaagaac tccaacaagc ggtatcgaaa
     4261 tgccaggatc gttttggtga agttacgatt ttactgaata catcggggat attggatgcc
     4321 tacaaaccat tgatggaaac tgacgaatcg ctgtgggatc ttatttatga gacgaatgtg
     4381 aaaagcatgt atcagctgac aaaaatcatc cttcctgata tgatccgccg aaaaagcgga
     4441 acaatcatca acatggcttc gattgcgggt ttggttgctg gcggaggtgg gatcgcttat
     4501 acaagtgcga aacatgcaat cgtcggattt accaaacaac tcgctctgga ttacgcagga
     4561 cagggtatcc gagtaaaagg aattgctcca ggagccatcc aaacaccaat gaatgcggct
     4621 gattttgaag ggaaaggtga gatggctgag tgggtcgcaa atgaaacacc agtcaagcga
     4681 tgggcacagc cggaagaagt agctgagctg acgttatttc ttgcctctcc tcaagcaagc
     4741 tacatacaag gaacgatcat cccaatcgat ggcggatggt tgttaaaata accttgtgtt
     4801 tttttcaagc ttctgtatga taaaactaaa gagagaaaca acggttgatg ataaaagaaa
     4861 gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt ctttttaata
     4921 atcgttaata ttcgaaacta agcggctttt cacgaccttt tgatataaac ggtgttcaaa
     4981 agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct atttttattt
     5041 gactattctt attacttgtt gcagggcagc tttttcacta ccttctgcgg cacttcagtt
     5101 ggaattttct tcccatcctt tggtcgcagt cattaccaag ataaaattat cttgcaaagg
     5161 agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca acgacagatg
     5221 tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt ttgtctgtct
     5281 ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg tccttgtata
     5341 acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatg gcttctcctc
     5401 ttggactgat agatgttgta gtaggaagcg tcagcacgct gcttgtattg cttatcaact
     5461 ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggctgtgaca gcatgcgtat
     5521 ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa ctaccttttt
     5581 tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc ggtgggataa
     5641 ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt taatttaaaa
     5701 tatggcagcg tggaagagac tttgagtctt cttccatgct gcttttttga ttaatttgta
     5761 tgaagaaatc gatttagccg ttttttcatc aagataccga tataagctgc tccagttgcc
     5821 ttgattgctt ccggcagtaa aaacggcagt agtccactgg caaatgcagt tgtaaaagta
     5881 agatcaccac ttattttcag ccagatcgtt ccgcagatca atgcagcaag agagcccatt
     5941 agattggcaa agattgcata taagtaatga gcatccgttc tttcgatcat ccaaccagtt
     6001 atccacgttt gtaagagaaa tccaatcaaa tatccgccag ttggcccaaa taaaacagct
     6061 atacctccag tcatccctga aaatacagga agaccaatca agccaagtaa aagataaact
     6121 acaacagata tcaatcctag ttttcttccg agaaaagttg ctgttaatcc aatagcaaat
     6181 gtttgccccg tcaaaggaac caagccaaaa ggaatggtca tctgagaaag gacagaaata
     6241 atcgcggcaa attccgcagc aagtattaat tctttcaatg ttcggttcat aatgccctct
     6301 tttcgttaac ttaatatttt tatttggtta acgaaattgt aaaacaaagt gattactttg
     6361 taaatagaaa atataaaaaa agaccagcta tccaatagat agcggtcttt ttcaagaaag
     6421 cttttgaaat tataagcttt ggttgtagta ttcaacgaca agagcttcgt cgatttctgg
     6481 atatagttcg tcacgttctg gtaaacgagt caatgaacct tccaattttt cagcgtcaaa
     6541 gcttacgaat gctgggcgtc caacagttgc ttctacagct tcttttactg ttacgatgtt
     6601 ttgtgatttt tcacgaactg agatcacttg acccacttct acgtggtatg aagggatatc
     6661 aacgcgtttg ccatcaacta aaatgtgacc atggttaact aattgacgtg cttgacgacg
     6721 agtcgttgca agtcctaaac ggtaaacaac attgtctaaa cgttgttcta gtaagatcat
     6781 gaagttaaca ccatgtttac cttctttgat tttgctagct tttacgaaca agttacggaa
     6841 ttgacgttcg ttcattccgt acatgtgacg aagtttttgt ttttcagtta attgcatgcc
     6901 gtattctgat tgtttgccac ggctgttagg tccatgttga cctggtgcgt aagggcgacg
     6961 tgctaattct tttcctgtgc ctgacaatga gatgcctaag cgacgagaaa ttttccatga
     7021 tggtcctgta taacgagaca ttaaaatttc ctccaatgaa tataagtttt tggagtaaaa
     7081 taatccgttg aaaaattcat aatcatatag tttgttcttc aatcttcacc tttgcagccg
     7141 cggttacaca atcgaacccg agacggggca acaaactagg gatgagttat tatttttctg
     7201 ctgcattatt ttacacaaac tacagtatat gatagatcat ccacgaatgt caagataatt
     7261 agtacataaa aacgctttac aaacctaggc tgtctttata aaatgagagg gcatgtgcaa
     7321 atggaaaaaa gcgaaggaag tttaaggaga aaatcaatga atccggtatc accattattt
     7381 gagaaaatcg atgcttcaat tgaaaagaag gcgaatcttt ttgaaaacag cttttcacgt
     7441 tacgctgttc gagcggttct cgcctgtttg tttttgactt taggaacggc tgtggcattt
     7501 gcaatcgcga tgaaggggga agatatcgcg catggtctag gaaagatttt atacgcattt
     7561 atgttcagct ggggtctggt catgattcta tacatgaacg ctgaattagg aacatcgaat
     7621 atgctttata tgaccgtggg tgtttttcgg cagagacttg ctttaagtaa ggcagccaag
     7681 attctatttg cctgtatttt tttcaatatg attggcggta tattatgtgg atttcttatt
     7741 tctatgacta cgccatttca agatttaacg ttggacaact ttatgttaga atcaatagca
     7801 gtaaaactag caaaacctgc agcaacgatt ttggtggaag ggatgtttgc gaatatcgtt
     7861 gtcaataccg ctgtactgat cagcatgcgg atgaaagagg atgccgggaa agtgatcaca
     7921 gtcgtattca tcattttcat ttttgctttt ttaggatatg aacacgtgat agcgaatttt
     7981 cctgccttta cgcttgctta ctttgcttct caaggacaga cggatggaat gacagtcagc
     8041 aatgtgttac ataatttgtt ttttgctttg gcaggaaatt atattggtgg cggtttagta
     8101 atgggactgg gatatgcttg gctgaatcag tcaaaatcat cctatgtgga ctaaaaagtc
     8161 gttcattaaa ggggaagtgc catggaagtt ttagtttttc caaaaaatga ggaaaaagcg
     8221 ttacaaatgg aaaagtacat gaaaaatctt ttttcctttg ctggagtacc taagccggaa
     8281 cgcgctcttt tacaacaacc gttactggat gagagcaaaa atttatctat ttctgaagtt
     8341 ttagcttttg tttattatta ttatgaacaa atcgaaagag agtatcagta tgtagcgatc
     8401 gatttagtcc agaaaaacat caaacggctt acatttgaag acttagtcgt attgttgcca
     8461 ctagttcaga aaaaagaatg gtgggacagc atcgactctt ggagaaaagt atacagcaca
     8521 tggtgtaaag cctacccaga gcagcgtgaa gaagtctttc aattgtttga aaaaaaggaa
     8581 gatttttggc tacgtcgaat agcaatcacg ttgcaattag gttataaaca gcaaacagac
     8641 acaggactat tgatccgagc aatagacgaa gatcgtcaaa caaaggaatt ctttatccaa
     8701 aaagcaattg gttgggcatt acgcgattac agtaagaccg atgccgattg ggtaaaaaga
     8761 cagctgacaa cagatttgag taagttagct gttcgagagg caagtaagta tttataagaa
     8821 agcgagacga caagagatga gacgcaaatt atttgctttt gatattgatg gaacgttgtt
     8881 agattcaaac aaacaagctt tagatagcac gagagaagca ttggaaaaat tacgcaaagc
     8941 cggtcatctg gtcacgattg ctacagggcg gagccgattt catgcgcaag agatcattcg
     9001 cgatcttgat tttacgaact acattttatg taatggagcg gccggttttt tagaccatga
     9061 acaagtatat aagaatttac tagatcgcga ccagttggat gcgttcgttc aagaagcgca
     9121 tcatgagcag atcgatacag cttttgtcag cttagacaat atcaagcgtt cttcttccaa
     9181 tgatgtaacg gtgatggaag aagcgatgca ttctttcggt gctgtcttgc ctgaattaga
     9241 tcagtttttt gcagaggaac aagaaatata ccaagcgtta gcatttttcg atcaaacata
     9301 tgatgggaag ttctcttcat atgataaaat tcgatttgtc cgctggcatg agaacagtgt
     9361 agatgtcgta cctaaaaatg gttctaaagc agcgacaatt ctgcacttag cagaacaagt
     9421 aggcatccct catgaagaca tcatcagctt tggcgatgga caaaacgacc gtgaaatgtt
     9481 gcgcatgtca ggaattggga tagctatggg gaatgctgtg ccagaagtac aagcagaagc
     9541 taagatggtc actgatacaa acgatcagga tggaatttgg aaggcattga aagagttaag
     9601 cgtgatttaa aagaggttgt gaaaaagctg ttctgcatca agtaataagc ccagccaaac
     9661 aaaaatagct cctcctattt ttcgtttttc tgggcttatt accgcagatg caagcttttg
     9721 aacaccgttt aaggaagagg tcgtgacaga agtggtcagt ttcaagaaat aagaagggat
     9781 ttacgaaaat tgcttctcaa atttttgtaa aattacggct tatttctgaa gaagtagatg
     9841 aaacaacgtt tattcgggaa atgggactgt gacaagatgt ttgtcatagt cccatttttt
     9901 tattttattt ttcaaaacta agaaatacca ggaaagagtt ccttttgttt gaccttttct
     9961 ttcttattga aaacaactat aatatagagt gatgttaaaa gagaagagga atttagtgtg
    10021 cgggtattgt ttattggtga tgtagtaggt tcaatgggac gagagatgat tactgaatat
    10081 cttcctagat tgaagaagaa gtaccgtccg caagtaacga ttgtaaatgg tgagaatgcc
    10141 gcttctggac gtggaatcac agaaaaaatc tataaaaaat ttttgcaaga cggcgtagat
    10201 gtcgtgacga tggggaatca tacgtgggac aaccgcggaa tctttgagtt tgtaaatgaa
    10261 gctaaaaaga tggttcgtcc ggcaaatttt ccggaaggga cgcctggaca agggatggtc
    10321 tttgtcaaag tgaaccaagt cgaactggca gtgatcaaca tgcaggctcg ttcttttatg
    10381 gtggacttgg atgatccttt tcgtaaaatg aatgagctgg tagaagaagc cagaaaacgt
    10441 acgccgatca tttttgtaga ttttcatgga gaaacaacaa gtgaaaaaca agcaatgggc
    10501 tggtttttag atgggaaagt ttcagcagtg gtcggtacac atacacacgt tcagacaaat
    10561 gatgcgcgaa ttttgccaag aggaacagcg tatctgacag acgttgggat gacaggtccc
    10621 tatgatggta ttttgggtat gcgcagagaa cctgtaatcg aaaagtttct gaccgcatta
    10681 ccaaaacgtt ttgaagttgt ggaacaaggc cgatcgattt taagcggctg tatcttggaa
    10741 ttggatgata cgacaggtca tgcaaaaaaa atccagctga ttcagatcaa cgatgatcga
    10801 ccgttcatgg agtagaaaga gaggcagttt tttgcagcag gagccggaaa ttcaaaaaga
    10861 agtaaggaaa ctgacaaaac ttttaagaga gaatgaaacg atcattcgct acaaagaact
    10921 tgaagaaaag atccagcaaa accagtattt agctgagttg agagaaaaaa tcaaacaagc
    10981 gcaaaaagat gctgttcatt ttgcccacta tgacaaacca gcagcagaac aagaagcaat
    11041 caaacaagcg gatcaattca tgcaagaatt tgatcagcat ccgcttgttg tagcttatag
    11101 aaaacagctg ttggaagcag atgatctgct tcatcattta acaactatga tacaagaaga
    11161 aatcaatgga caaatagagg aggaaaaaca tgcctcaaaa aactaaaaat actcctatga
    11221 tggaacaata tctaagcatc aaggcccaat accaagatgc ttttttgttc tatcgtttag
    11281 gagactttta tgaactattt tacgaagatg cgatcaaagc gtcacagctt ttggaattaa
    11341 cgttgacgag cagaaatcga aatgcagaag aacctattcc tatgtgcggt gtacctcacc
    11401 atgcagcaca aggatatatc gatgccttga ttgaaaaagg ctataaagta gcgatttgcg
    11461 agcaggtaga agatccaaag acgactaaag ggatggtcaa gcgcgaagtg atccagctag
    11521 tcactcctgg tacagtcatg gacagcaaag gattgtcggc aaaagacaac aattttttaa
    11581 cagctgtagt tagtcaagga aatgaatttg gctttgctta tgccgattta agtacgggag
    11641 aattgaagac ggctcggttg catgatgaag aagctgtttt aaatgaagcc tctgcattgc
    11701 agacaaaaga actcgtatta ggcagtgaga tcactgatac ccttcgtgaa cagctaagcg
    11761 ggcgtttagg gctagtcttt tctcaacaaa atgagatgga agaaaacgcg gaatttagct
    11821 ttttgacaag cgaactagtc gatccgctga aaaaagaagc aaccggcaaa ctattgactt
    11881 atttatcggt gacacaaaag cggagtttag ctcatatcca aaaagcagta gaataccaac
    11941 cagatcattt tttgaaaatg gattattatt caaaattcaa cctagaatta agccaatcta
    12001 ttcggactgg gaaaaagcac ggcactcttc tatggttgtt agatgaaaca aaaacagcaa
    12061 tgggtggccg tttactaaaa caatggctag accgtccgct tatccaggca aaacaaatta
    12121 aagcaagaca agaaatggtc gcttctttgt tagacagcta ttttgaacgt atcgatttgc
    12181 agtcagcatt aacgaaagtc tatgatctgg aacgcctagc aggtcgagta gctttcggta
    12241 atgtcaatgg gcgagacttg atccagctga aaaattcttt agaacaagtg ccaatgatca
    12301 gagggttgat tgaaggaatc gatcaaggtc aatggcaaag tctgctttca gaaatgcagc
    12361 cgatggacca tctcgtccaa ttgatcgacc aagctatcca agatgatccg ccgttacaaa
    12421 taacggaagg aaacatcatc aaagatggat tcaatgagca gctcgatatg taccgttctg
    12481 ctatgagaaa cggaaagaaa tggttggcag aacttgaagc aaaagagcgt caagaaacag
    12541 gaatcaaaaa cttaaaaatc ggatttaacc gcgttttcgg ttattatata gaaatcacaa
    12601 aagcaaatct tggaaatgca gaactggaga agtatgaacg aaaacaaact ttagcaaatg
    12661 cagagcgttt catcacacca gaactaaaag aattggagac acaaatcctc gaagcagaag
    12721 aaaaatctgt tgatttagaa tatcagttgt ttttagctgt gagagaagaa gtcaaaaagg
    12781 ctatccagcc gctacaagta cttgcaaaag caatcagtgc agtagatgtg ctacaaagtt
    12841 ttgcaacgat cagcgaacgc tatcagtatg ttcggccaga acttgtcagt gacaagcatc
    12901 agcttctgat caaagacgga cgacatcctg tagtagaaaa agtattagga catcaggaat
    12961 atatccctaa cagcgtagaa atggcagaag acgaaatgat cttgctgatt acaggaccga
    13021 atatgtctgg taaaagtacg tatatgcgac agttggcatt aactgttctg atggcgcaaa
    13081 tgggctgttt cgttccggca aaacaagcag ttttacctat ttttgatcgg atttttaccc
    13141 gaattggtgc aagtgacgac ttaattgctg gacaaagtac ttttatggta gagatgatgg
    13201 aagccaatca agccctgcgt tatgctacgc caaacagttt gattttgttt gacgaactag
    13261 ggcgaggaac agcaacctat gacggaatgg cgttggccca agcaatcatc gaatatatcc
    13321 atcgtcatgt acaagcaaaa acactctttt ctactcatta tcatgaactg acggtacttg
    13381 agaaagaact tccgcagctt aaaaatgtcc atgttggagc cgttgaaaaa gacggagaag
    13441 ttgtcttctt gcataaaatg atggatggac ctgcggataa aagctacggc atacatgtgg
    13501 caaagatcgc aggattgcct tccaatctac tagaacgagc agcagatatc ctgcatacgc
    13561 tagaaaatgg cgataacgga caccatactg ttccagctga agtagaagag aaaactaaaa
    13621 cagccaaaca ggagcaaata aaaccacctg cagaaaaagt aaatcaagac catatacaag
    13681 accgtgtaaa agaagaaaac caacagcttt ctttattcag tgaattatcc gaaaatgaaa
    13741 cagaagtcat tgaatcactg aagcaaataa atttactaga aatgacgcca atggatgcac
    13801 taaacacatt atatgaatta caaaaacaac tgtaggaaga aggcggatat cgatggccaa
    13861 aatccaagaa ttatctgaac gtttagctaa tcagatcgct gcgggagaag tagtggagcg
    13921 accagcttcc gttgtgaaag aattagtgga aaatgcaatt gacgccggca gtactcagat
    13981 tgatatctta gttgaagaag cgggactgcg gacgatccaa gtgattgata acggagaagg
    14041 cattttggca gacgacgtag agaatgcctt caagcggcat gcgacaagta agatccataa
    14101 tcgcgacgat ctttttcgca tacgaacgct gggctttcga ggggaagctt tgccaagtat
    14161 tgcctctgtt tctgaaatga ctctggaaac ggcgaatcag gaagaaaaac aaggcaccta
    14221 tctgtcgctg aaaggcggag aagttattga gcatcgacca gcaccactgc gtcaaggaac
    14281 gaagattacc gtcagcaatc tgtttttcaa tacgccagcc cgcttgaaat acgtgaaaac
    14341 actgcacaca gaattagcga acatcgggga catcgtcaat cgactggcat tgagtcatcc
    14401 atcgattgct tttcgtttag tacatgatgg aaacaaaatg ctggcaactg ctggaaatgg
    14461 cgatctgaaa caaacaattg cgggaatcta cggattatct accgcaaaaa aaatgctgaa
    14521 aatcgaagga aaagatctag attttgagtt atttggctat gtttcattgc ctgaggtgac
    14581 tcgtgccagt aggaattact tatcgacgat catcaatgga cgttttatca agaacttttc
    14641 cttgaataaa gcaatcgttg caggttatgg gtcaaaatta atggtcggtc gtttccctat
    14701 tgctgtattg gaaatcaaaa tggacccgct tttagtagat gtaaatgttc atccgacgaa
    14761 gcaggaagtc cggctgtcga aggaaaaaga actaacagag ctaattagta acgccattca
    14821 aaaagcattg tctcaagaaa atctgattcc aagtgcagcg gataatctac ggtttaagaa
    14881 aaaagtacct gatcagccta aaacagaaca aatcgaaatc gatttgtcct acgcttttga
    14941 agaagaaaaa cctaaaaagc caagtagttt gaattttgat cgagagacag gtaccttttt
    15001 cgtagaagaa ccaaagaaca catttgttga cagaattgtt gataagtctg tggaaaatgt
    15061 tgaaaactat tctggttttt ctgaagaaaa caccacttat ccagaaaaaa cagcgaaaac
    15121 ggtggataag tttggtgaaa atgttgataa ctttgtagaa aaaacagtgg aaaaatctga
    15181 aaagaaagaa tttcccgttg tggataacat ggagaaaaca aatgaagaac aagcagataa
    15241 atccgtagaa gaggatgaaa agatttacga ggaagaattg acactccatc ctgaatttga
    15301 tttcactcaa ggaaacgaca aaacattaga acaagccatc aagaaattag aaacggaaaa
    15361 accagtacaa cgttttcctc agttggaata ttttggacag atgcacggaa cgtatctttt
    15421 tgcccaaagt agcgatgggt tgtacatcat tgatcagcat gctgcccaag agcgcattaa
    15481 gtatgaatat ttccgggaaa aaattgggga agtatctgat gatctgcaag aattgcttgt
    15541 tccatttgtg ttagactatc caaacagtga tgctttgaaa ttaaaagagc aaaaacactt
    15601 gctagaagat gcagggattt atttagaaga atttggtcaa aacagcttta ttgtacgggc
    15661 ccatcctact tggtatccca atggagaaga agaatcgatt attcgggaga tgatcgatat
    15721 gctgctgaca actggaagtg tgagtgtaaa aaaattccgt gaagcaacag caatcatgat
    15781 gagttgcaaa cgatcgatta aagccaacca ttatttgaat gaagcacaag cacgtgtgct
    15841 gctgaaggat ctggcacgat gcgaaaatcc tttcaattgt ccccatggcc gtccagtatt
    15901 gattcacttt accaattcgg atatggaaag gatgttcaaa cgaatccaag acccgcatta
    15961 agtcaaagag aagagggaaa acatgaagat tgtattagca tcccaatcac caagaagaaa
    16021 agagttattg gctcatatcg tttcagaatt cgagattgaa cctgctgata tcgatgaaac
    16081 gccatttcca gacgaacagc ccgtcgatta tgttcgtaga atggctgaag aaaaagcacg
    16141 aattgtctgg gaacaagatg aaaaagaaga ccatttagtg attgccagtg acacgactgt
    16201 tgttctcaat caagagatca tgggtaaacc agaagatctg gctgaggctg aattcatgct
    16261 gaaaaaacta agcggggaaa cacatactgt atatacagct gttgttttaa aaacaaagga
    16321 acgagaagaa cggatactag cagaagcaca tgtcactttt tatcctttga cagacgaaga
    16381 gatcaaacgc tacttggaga cgggggatta tgcggataag gcaggggcat acggcatcca
    16441 aaatgctgcg agtgtattcg tcaaagaaat ttctggagat tattacagta tcgtaggatt
    16501 tccgattggt gctgtcaatc aggcactgaa aacattcatt taaacaaaaa agcggactat
    16561 ctgattttcg attgaaaatc catgatagcc cgtt
//
LOCUS       DACKAC010000063        15936 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.064, whole genome shotgun sequence.
ACCESSION   DACKAC010000063 DACKAC010000000
VERSION     DACKAC010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15936
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.064"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(15..1154)
                     /locus_tag="I0B46_002121"
     CDS             complement(15..1154)
                     /locus_tag="I0B46_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAR2084394.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1553..2851)
                     /gene="eno"
                     /locus_tag="I0B46_002122"
     CDS             complement(1553..2851)
                     /gene="eno"
                     /locus_tag="I0B46_002122"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAR2084395.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(2967..3722)
                     /locus_tag="I0B46_002123"
     CDS             complement(2967..3722)
                     /locus_tag="I0B46_002123"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAR2084396.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(3826..5016)
                     /locus_tag="I0B46_002124"
     CDS             complement(3826..5016)
                     /locus_tag="I0B46_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAR2084397.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            complement(5126..6127)
                     /gene="gap"
                     /locus_tag="I0B46_002125"
     CDS             complement(5126..6127)
                     /gene="gap"
                     /locus_tag="I0B46_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAR2084398.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            complement(6168..7205)
                     /locus_tag="I0B46_002126"
     CDS             complement(6168..7205)
                     /locus_tag="I0B46_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAR2084399.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            complement(7408..8742)
                     /gene="rpoN"
                     /locus_tag="I0B46_002127"
     CDS             complement(7408..8742)
                     /gene="rpoN"
                     /locus_tag="I0B46_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAR2084400.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            8802..8873
                     /locus_tag="I0B46_002128"
     tRNA            8802..8873
                     /locus_tag="I0B46_002128"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8835..8837,aa:Arg,seq:ccg)
     gene            complement(9065..9949)
                     /locus_tag="I0B46_002129"
     CDS             complement(9065..9949)
                     /locus_tag="I0B46_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAR2084401.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNYTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(10036..10935)
                     /locus_tag="I0B46_002130"
     CDS             complement(10036..10935)
                     /locus_tag="I0B46_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAR2084402.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            complement(10952..11458)
                     /locus_tag="I0B46_002131"
     CDS             complement(10952..11458)
                     /locus_tag="I0B46_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAR2084403.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            complement(11647..12267)
                     /locus_tag="I0B46_002132"
     CDS             complement(11647..12267)
                     /locus_tag="I0B46_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAR2084404.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            complement(12343..12780)
                     /locus_tag="I0B46_002133"
     CDS             complement(12343..12780)
                     /locus_tag="I0B46_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAR2084405.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            12943..13773
                     /locus_tag="I0B46_002134"
     CDS             12943..13773
                     /locus_tag="I0B46_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAR2084406.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            13757..15124
                     /locus_tag="I0B46_002135"
     CDS             13757..15124
                     /locus_tag="I0B46_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAR2084407.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            15143..>15936
                     /gene="yidA"
                     /locus_tag="I0B46_002136"
     CDS             15143..>15936
                     /gene="yidA"
                     /locus_tag="I0B46_002136"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAR2084408.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAI"
BASE COUNT         4310 a         3333 c         2938 g         5355 t
ORIGIN      
        1 ttattaaaaa aatcttacgc taagtaggat aaaataattc cttgatattt ttcgatcata
       61 tccaaataat tatcgatttc aacatactca tctacttgat gaggcaaagt aacgacacca
      121 ggcccaaaga cgacaaagtc gaaggaatgg ttagctttcg taaattcggc tgcatccgtt
      181 gttgctgccg ctccaactaa aggtaatggc tggctgaatt gttgctgaat gcaatgaatc
      241 aaaggagaat ctggatcagc tttgacaggt attttgttgt aatcgatcat caattccaaa
      301 tgatagtctg tctcttgatt caattcattg acgattgatt gcagcaatgc aatgatttta
      361 tcatttgggt attctggaat cgatcgaatg ttcccttgga gctgcgcatg gcttgggatg
      421 ctgttgactt gattcccgcc actgatcaat gtaacattat ggattgtttt tcctaggaca
      481 ggattctcga ttgtttcagc gaggtgattc atttcagcat ttgctttcgt aatgaattca
      541 tttaggtgat tgattgcatt ataaccttgg tctggcatag agctatgcgc ttcttttccg
      601 tgcgaggtga ctgtatagtt gattgatccc atgtgcgtat acatcaaact gtaattggtt
      661 ggctctccga tgatcaaggc atctaagtca tctacatagc ctgctttcgt gagctgttcc
      721 ccacctagtt cacccacttc ttctccgaca gtggctaata atttgactgt tccgttaaat
      781 ggttttcctg actctttcag ttcgatcatg gcaatcacca ttgctgccaa gccactcttc
      841 atatctgtag caccacgtcc gtacaaacgg ttcccatgga tttctgctgc aaagggtgca
      901 taggtccaag acgattcatc accagccgcc acgacatcca tatgtccaga taggcctaac
      961 acttttcctg attggccctt ttggtaacga gcaatcaaat tatctctacc atctctgtaa
     1021 gaaacaatct ctcctgttat gtcatgcttt gccaaaagct tattcaaata ggctgctact
     1081 tcaccttcgt ttccattcac tgatttaatt cggataatct cttgtaaaat cgcgattttt
     1141 tcttcttttt tcatctaatc ctctcccttg ccatttcttt ttattgtatt tgagtgtgct
     1201 cgaattttca aataggagca accaaagtaa aaatgaagaa agagagccac tttatgaaaa
     1261 atatgctgct acttccctcc tattttattt atacgagagg acttgcccat agattcctat
     1321 tctactgttc tatccttaaa agctcttata tgatgctagt tattgttagc tttccttttt
     1381 caaggatgat cttttcttgt accattcttt gtaaacatca tatacagaag ctagttgcaa
     1441 aaaaactctc tacctgctga tatttcagca agtagagagt tcgattactt aactaagtga
     1501 tcatactcga ctatctttca atagtcgatc atgtatcttc attagaaaag aattatttgt
     1561 tttttaagtt gtagaaagat ttcaaacctt tgtattctgc aacttcacca agttggtctt
     1621 cgatacgtag taattggttg tatttagcaa tacggtcagt acgtgataga gaaccagttt
     1681 tgatttggcc agcgtttgtt gcaacagcga tatcagagat tgttgaatct tctgtttcac
     1741 cagaacggtg agatacaact gctgtgtagc cagcttcttt agccatttcg atagcttcga
     1801 aagtttctgt taatgtaccg atttggttca ctttgataag gattgagtta gcaattcctt
     1861 tttcgatacc ttcagataat ttttgagtgt ttgttacgaa caagtcgtca ccaactaatt
     1921 gaactttgtc gcctaatacg tcagttaatt tcttgaatcc gtcccagtcg ttttcgtcta
     1981 atccatcttc gattgagatg attggatatt tagaaactaa ttcttcgtag aatttgatca
     2041 tttcgtcagt tgttttttcg ccttcgcctg agtcagctag tacgtaaaca cctttttctt
     2101 tgtcgtagaa ttctgaagaa gcagcatcca tagcaagaac aacgtcttta ccaggtacat
     2161 agccagcttt ttcgattgct tcgatgatta cttcaaaacc ttcttcgttt gaaccaaggt
     2221 ttggagcaaa tccaccttcg tcaccaacag aagttgctaa tccgcgagct ttcaagattg
     2281 aagctaatgc gtggaatact tcagcaccca tacgtagagc ttctttgaat gttggagcgc
     2341 caacaggcat gatcatgaat tcttggaagt cgattgagtt atcagcatgt gatccgccgt
     2401 tgatgatgtt catcattggt gttggcaata cttttgtgtt gaatccgcct aggtagtggt
     2461 ataaaggtac ttctaggtag tcagcagctg cacgtgcaac agcgatagaa acacctaaaa
     2521 tagcgttcgc acctaattta cctttgttag gagttccgtc taaagcgatc atagctttgt
     2581 cgatagccat ttggtcacga acatcgtagc caatgattgc ttcagcaatg atgttgttta
     2641 cgttgtcgac agctttagtt acacctttac cgccataacg agctttgtct ccgtcacgta
     2701 attcaactgc ttcgtattca ccagttgaag cacctgaagg aaccattccg cggccaaaag
     2761 ctccgctttc tgtgtatact tctacttcga ttgttgggtt accacgtgag tctaagatct
     2821 cgcgagcgta aacatcagta ataattgaca tgttttgtct ctcctttgag tttgtttttt
     2881 cctaagggat ttccctcata tacgatttta gttaattttg ctcattagtg caaacatttc
     2941 ttgcattttt cgcaaatttt caacaattat tttacagcgt ctaacaatga caagaatgaa
     3001 tctgcttgca agcttgcgcc accgactaaa gcaccgtcaa cattttcttt tgccatgtat
     3061 tcagcaatat tttctggttt tacagaaccg ccgtattgga tacgaactgc ttctgatact
     3121 tcttttccat aaagtttttc aactgtttga cgtacaactc cacaaatttc gtcagcgatt
     3181 tgtgcgtcag ctgatttacc agtaccgatt gcccagattg gttcataagc aataaccatg
     3241 tttgacacct gttcagctga aagatcagct aaaccagctg taatttgtcc ttcgatccat
     3301 tcagcagttt tgcctgcttc ataggtttct aaagattcgc cgcagcatag gattggtgtc
     3361 ataccatttg caaagatagc tttcgctttt ttattgatgt cttcgtctgt ttcatggaaa
     3421 tattcacgac gttctgaatg tccgatgatc acataatgaa cacctagatc agcaagagct
     3481 gctggtgaag tttcaccagt gaacgcacct gagttttccc agtagcagtt ttgtgctgag
     3541 atttttagtt ctgtaccttc agctgatgtc attaattctt gtaagaataa tgcaggagat
     3601 ccgatgactg aatcaacttt gtcatttgaa gggattttgt tttttactgc ttcagcaaat
     3661 tcttttgctt ctgatgcagt tttgttcatt ttccagttac ctgcgataat aggtttacgc
     3721 attgaggaac acactcctta atttattgaa aaatggctag ggagtcatga aaactccctt
     3781 caccttttgt tcgattactc gtcagaattg agcaaacttg gtcgtttatt tatcgttgat
     3841 tgctgctagt cctggtaatt ctttaccttc aagcaattcc aagcttgcgc cgccacctgt
     3901 tgagatgtgt gtgaatttgt cagcaaatcc taattgttct gctgctgcgg ctgagtctcc
     3961 gccaccgatg attgttgttg cgccttctaa gttagcgatt gcttcacaaa caccgattgt
     4021 acctttagca aagttactca tttcaaatac acccataggt ccgttccaaa caactgtttt
     4081 tgcaccttgt aatgtatcag caaataattt tactgatgct gggccgatgt ctaaagccat
     4141 caaaccatca ggaatgtctc cttcgattac ttgtgtttca acatcatttg aaaattctgg
     4201 tgcacagaca ttgtcgattg gcaagacaag tttgtcgcct gctttttcga ttaattcttt
     4261 tgctagttcc actttatcag cttctactag agagttaccg atcttgattc cttttgcttc
     4321 gtagaatgtg taagtcattc cgccaccgat caagatttta tctgctttag gaatcaagtt
     4381 ttcgattacg ccgattttat cagaaacttt tgcgccacca aggattgcaa tcattggtcg
     4441 ttttggttct tctactgctt caccgatgaa cttgatttct ttttccatca agaatccagc
     4501 aactgttggg ataccagttg aagcgatacc tacgttagaa gcatgtgcac ggtgagctgt
     4561 accgaaagca tcgttaacaa atacatcgcc taatgaagcc cagtatttac ctagttctgt
     4621 atcatttttg ctttcttttt taccgtcaat atcttcaaat cgtgtatttt cgaatacgac
     4681 aacgtcgccg tcattcatgt tgcggattgc ttcttctaat tgttctccgc gagtttcagg
     4741 tacgaatgtt acttctttac ctaataattc gcctagacgt tttgcaactg gagctaaaga
     4801 ttttccttct ttatcttctt ctgtttttac acgtccaagg tgagagaaaa ggattgcttt
     4861 tccgccttgt tctagaacgt agttgattgt tggcaatgct gctttgatac gagtgtcgtc
     4921 agtgatcacg ccgtctttca aaggaacgtt gaagtcaaca cggacaagaa cttttttgtc
     4981 ttttaaatcg atatctttca ctgttttctt agccatttta tttcctcctg aataaactta
     5041 ttatcaaaaa aagcggaaga agcgcgattg cttccccgct tttaaatgta catgtctata
     5101 atttacactt gtacgtttgt ctatcttata agttagcgaa gtattctaaa gtacgtacta
     5161 attgtgcagt gtatgacatt tcgttgtcgt accaagcaac agttttaact aattgtttgt
     5221 cgccaactgt cattacttta gtttgtgtag catcaaataa tgaaccgtaa gtcataccaa
     5281 cgatatctga agaaacgatt tcgtctgtgt tgtatccgta agattcgttt gaagcttctt
     5341 tcattgctgc attgatttcg tcaacagtta cttctttttc aagaactgtt actaattctg
     5401 ttaatgaacc tgttggtaca ggaacacgtt gagcagctcc gtctaattta ccgttcaatt
     5461 cgggaattac taaaccgata gctttagcag cacctgttga gttaggaacg atgtttgcag
     5521 cagcagcgcg tgcacggcgg aagtcacctt taggatgagg tccgtctaga gtcatttggt
     5581 cacctgtgta agcgtggata gttgtcatta atccttcaac aacaccaaat ttgtcgttca
     5641 atgttttagc cataggagct aaacagttag tagtacatga agcacctgag ataactgttt
     5701 ctttaccagt taatgtttcg tggtttgtgt tataaacgat tgttggaaca tcgtttccgc
     5761 caggagcaga gataacaacg cgtttagcac cagctgttaa atgtttttca gctgctgatt
     5821 tagaagtgaa gaaaccagta cattctagaa cgatatctac gcctagttcg ccccatggta
     5881 attcttctgg gttgcggtta gctaatactt taacttcttt tccgtttacg ttgaatgaac
     5941 cttcatgaac ttcaactgtt ccgttgaaac gtccttgagt tgtgtcgtat tttaacaagt
     6001 gtgccaacat tttagcatct gtcaagtcgt tgattgctac tacttcgatt ccatctacat
     6061 cttggatacg acggaatgct agacgtccaa tacgtccaaa accattaata cctactttaa
     6121 ctgtcattta agatttcctc cttatgaaaa tcaaaaaaat atttatttta aagggttacc
     6181 ccttttaaaa tctcatttgc agcagcttca tcagtgatga gccacgtttg ttttggtgca
     6241 tttttcatat aagctcgaat tgctttagct tttgattttc cgcccgcgat tgccatgatg
     6301 atcgggactt tttccaaatc ttgcaattct atgccgattc tagggacctt ataaacgact
     6361 tcaccttttt cattgaaaaa gtagccaaaa gattccgcaa cagcatttgc ttgtttcagc
     6421 atgactaatt ctttttcagt catcttacga cgtgctgcca tgtgtaatgc acgcccaata
     6481 ctatgaataa cacaattcgc ttcactaatc aagtttaaga cttcttggat agatggctca
     6541 ttgagtaaag aattgtaagt tgccagactt aattgttccg gaacataaag tgcgcggtgg
     6601 ttaccgtttg ctttagcagc cattcttgca ctgacagaat ttgcctgtac agttatcgct
     6661 tcaccaatcc cgcctcttgc agggacaaac aggttatgcc gtttcttatt ttccagatta
     6721 gacaattgtt cggctaccac agccatagta gttcctccca tcacagcgat gatattctcg
     6781 ccttctggaa gtagtcggtc taaggcttcg ctcagcgctt cgccgaatgc atcagccact
     6841 ttggtctgct cttcgctgtt tcctgaaaca acgatacagc gttggttacc aaaatattct
     6901 gccaactgtt gttcggtttg atgcgtacct aaaagctgat ccatgaagtt ttcaaggctt
     6961 tgataaactt cttcgccctt agttgtcagc gtcataccgc tttttgaggt gtcgatcaaa
     7021 ttcagatttt tcaaaaggtc tgtttctgtt cgtaagactc gttctgtgat ccccatactt
     7081 tccgataaac ttcgacgccc aatcggctgc atccaataga tatttcgtaa aattttatag
     7141 cgttcttgca acacttcgat aatatctggt gcaacagatt caatcatttt tatttcatcc
     7201 agcatagcga tctccttagt tggacacttt acgtccaatg tagacttaaa acgacccata
     7261 tacgataaaa aaagaaatta actattcgaa tttcacatca ataaagggaa atctcaacct
     7321 cacgactgta agtctaacag tttcgctctc gtttttcaac taaaaagact agaaaaagtg
     7381 aaatactttt tctagtcttt tgactgatta gttatcataa cgttttcttt ttgatgaact
     7441 cgggattcct aataaatcac gatattttgc tactgttcgt cttgagatcg ctatctcatc
     7501 ttttttcagc aattcaacca atttctggtc cgacaacggc ttgtttttgt cttcttgatc
     7561 gactaattcc tgcagctttt tcttcgctgt atctgctgaa aggtcttctg tctgttctgt
     7621 actattcgta ggaattcgtg tagtgaaaaa tttttttaat tcaaatacac cgaaatctgt
     7681 ttccaaatat ttcccgttga ctgcacgact gaccgtggat tcatgcacat ctatctcagt
     7741 tgcaacttcc tttaacgtaa gaggcttgat tggacgttcc ttgtcgataa agaaagcttt
     7801 ttgccgggaa acaatgactt gagcaacccg caaaattgta tccccacgtt gtaggatcgt
     7861 tttcttcaac cattcgaatt cctgtttccg ttcttttaaa taattttcag tttctttatc
     7921 tgcttgttgt gacatttgtt tgaagtaact ctcttgaaag cgtatatttg gctgattttt
     7981 tcggtttgag ataacttgta catttttatc gtcgtccact aatacccgga catcaggaat
     8041 aatgtacaat tcgctgctac ggtcgaaaat ccggccaggg cttggactca acgtttgtaa
     8101 atagtcgaat atctgctgaa cagcatgtag atcaacagaa aaacgttgtg cgatttcttt
     8161 ccattttcgc tccaccagtt cctcaaaaca ttcttccaac acgatataag ctaattccgg
     8221 cgcataatca tctcgttctg tttgaagcat gagacattct tgcaaactac gtgcacctac
     8281 tccagctggt tctaattgct gaatcagagt caacgcatcg agcatttgga taggcgtagc
     8341 tccaatctct tttccggctt cttctaaact aatcgtcaaa aatccgttca aatcgatgta
     8401 ttctacaaga taaagaacga ttttcctcaa aaaagtatct cggtaattca aatggacttg
     8461 ttcgatcaat gaatcaaata aagacccttt tgtatcagga atctgattta ggtaatttgt
     8521 ctcttctcct tcataagaag atgaagaagc ttttatgtaa tctggctgcc attccggctc
     8581 aacaacttcc accaatggat tttccattgc ttgattctca acgaatgcca acagctcctc
     8641 actgttatat tgtaaaactt ggatggactg ttgcaactgt tgcgtcatgg ctagtttctg
     8701 tgtctgctgc tgtttttgtg aaaaattttg ctcaaatttc atttttttgt ccaatcccct
     8761 tgtaattttt tatctgttta gttacaatta tcccagtgct tgcgtcctta gtgtagtgga
     8821 tatcacgtaa gattccggtt cttgagacgg gggttcgatt ccctcaggac gcgttctact
     8881 gctattttag cataatttac gaatagttgc ttctttattt ttaagtgaaa actgtccgcc
     8941 aaagaaaatt ggtggacagt tttttgttgt acctgtatag atattgttaa gcctttgcaa
     9001 gtaaggtagt tatgtgttca actacttatt aaaatgtgtt atttattaaa gatacttgtc
     9061 tcacttactg aactggccga tgatcgtttc tgccataata gttctcgaca gccgttggat
     9121 gccctaataa atccccaact gtcgtgatgc gatacccttg ctctttcaga taatcgatca
     9181 cttgaggaac ggcacgaatc gtttcaggat agatatcatg gaataaaata attgccccat
     9241 cgtatactgt tgcttggatc ttctgcaaaa tcaaatcagg atttcttgtc ttccaatctt
     9301 ctgaatcaac agaccattcg atgatcggtc gctggatgat cgttgctcct acattcgtga
     9361 cagaaccata gggcggacga acaaaagtgg gggttttgcc agtgactttt ttgatctcag
     9421 tgtctgcttt cgtcacttcc tccgtcatcg actgggcaga aagagtggcc agattctgat
     9481 gatcgtatgt gtaattgcca atctcgtgcc ctgcttcagc tgtctgcttc acgatttcag
     9541 gatgctgcgc cgtattttct cctaagacga aaaaagtggc atgcacgttt ttttctttta
     9601 agattcgcag aagctgcggt gttgtggtcg ctcctggtcc atcatcaaag ctgattgcga
     9661 cgcttttgac agccggatct cggctcgttt ttgtactgtc ttgtgttgat tgtgatgact
     9721 gactttgtac cttttccgct gattcttgaa tggcttcttt gacgttagct gtttcagttg
     9781 attggctttt aatggttgat tgttctgatt gtttgaaaga ttgtgacgaa tgctccgaag
     9841 agtgtgtttg tccttttact tcggtgatca aggaggccac cgaagtaatt tcgaaaaaca
     9901 aaagcacgga taaagcaata atactgataa ctaaataatt tcttttcact atatctcttc
     9961 tttctctttt tctcttctta ttatttactt gtacttactt gaaaattgaa actgctagtc
    10021 gcaactactt ctcgtctaat gatccaaata atcttccgat gccaaaatcg tttttgtcac
    10081 ggacttagga atgacaaatt ctggtgtacc catataacca ggagcaattt cgtattgaga
    10141 aaaagtcaaa actaattgat gctctttgtt caaataaaaa cttcgtgtag gcgtaacaag
    10201 tgttggtttt tctatcgtcc cttcttgaat atcttgctca gtccaatagt acttagatgg
    10261 atcttccttg acttgttcgg ccatctgccg tttgacttct tttgtcaaaa cagatagata
    10321 ggtgtcatct ttgaataaaa gtggaagtga gagctggaca ctgtttttct tgtcgattgt
    10381 gtcgtatctt ttttcaacat ggctatctgc tgctgtttgg gtgatctgcc tttcaatcac
    10441 gagaaaccgg ttatcgtcta cgattttctg ataatctccc aaaacttggt aatgatccgt
    10501 ttcaaaattt ccatattctt tttcagcttt ttcaaaatct gcttgtcctt cgcgaaagta
    10561 tttttgattt aatccatgaa ttgttttatt ttctttctca ttttgtgtat tgatcttcgg
    10621 tacctgaata tcgatttttt cagatcgatc agtaaagcgt tcgcctaaga tcaattcaat
    10681 gactgatcct aagatgggta aatcttctgc aaagcttcga acttgactat taaaaagaag
    10741 gctacttgtt gtaatcatta cgatagccaa agatactccg aacccttgga gacgtctctt
    10801 tctccgcttt gcttttataa atgattgctc ttcgataata aaacgctggc ggatctcctc
    10861 tttaagccct ttgggaattg cttgttcttt ataagttttc tttagttttt ttaaattttc
    10921 tttttgggaa tacatggcac tcttctctcc ttcattctag gatttgacgt aaagcattca
    10981 atgagcggta cattttcgtt ttaactgtgt tttcattcat atccaagata gtcgcaattt
    11041 ctttcaaacg aaatccttca aaaaagtaga ggatcagaat ctctttttcc ggactgtcta
    11101 ttttatctaa aatcatatgg agctctgcaa cggaagtttc taaaggtaac tgttggggga
    11161 tatcttgaat ttcaaataag tccacaggcg tccttttttg tctgcgccaa ctatctaatg
    11221 ccgtgcggat caatatccga taaaaccatg cactaaaatg ctcgatctct ttttcttttt
    11281 caaaatagtt cagggcttta tgaaaactat cttgcatcac atctaaggcc tcttctttat
    11341 catgtacgta attcatcgtg atacggaaca ttttttgata atcagtttcg atgagtccat
    11401 tcagcaggtg acggtatttt tcgatctgcc gttttttctt atatccagta aaaatcattc
    11461 tccactcctt tctgcctcac ttaatatgac gtacaaggtg cgctaaaagt ttcaattttt
    11521 ttatttttta tgtatttact tgggattttt atatacaaaa aagaaggtgt gacaaaaatc
    11581 gtgtcacacc tccttattct gataggctga tcgtctctgt cacaacctct cgtaagaaaa
    11641 taatgtttat tctttttcag agtcagaaaa ttcttcctct tcataatctt catcatcatc
    11701 gtcttcgtct atgatactta aatcttcttc gatatttcct ggcaagtctt cttcatcatc
    11761 tgtcgggtca tctgctccga tttcttgcaa gtcagaatta taagcagcga tttcttcatc
    11821 ttcatcatca tcgtcatcaa acagatcatc gtcatcttct tctgtcagat ctgtatcttc
    11881 tgggtcatcg tcattgtaat cgatcgcgtc ttcatcatca ttgataaagg cgttgacttt
    11941 cttgcgttta cggcgacgtg gagtatcttc atcctcttcg tccattccat ggtttacttc
    12001 ttcatcgatc gaatcgatag cataccatga acgcaagccc caacgattat cccctaatga
    12061 gataaagctg ccgtcaatat tcaagtcagt ataaaattgt gcgagtgaat cacgaatttc
    12121 actgtctgat ttcccaagat agttttggat ctggttagct aaatcagaaa aatccatgac
    12181 atcgccacgt tgttctaaga tcgcgtgtgc tacttcgatc attgataatt cgcttttgtt
    12241 tgcaccttca aatacactaa tttccaatca ggacacgtcc tttcacagtc tactccttat
    12301 catacaaaat cagacacaaa aaatcaaact tttcttttat atttacgctg taaaatccaa
    12361 ggtcaaataa tattcgccga ttttttccgg tcccatgaac agatcataat cgattcgaac
    12421 cttccctcct gtgggacggt ctttcaaact aacatgcagt tctttcgtaa aagtagcaat
    12481 ctggatcata ccgtatggtg ttcgatagga tgtttctaac cgttctttat agccaaattt
    12541 caaacgcatc cgcatttctc ctgcacggat cagctgtaca tgaccatctg gaactaattt
    12601 gatcgttaca ggaatctcat cgcccgtctc ctcctgaatc tctttataac gtatgtacag
    12661 cgtatcgcca attgtcacca tttgcccttt caaatcaaaa taaaaatctt gcacttcccc
    12721 ttgctgtttt acttttgtac gcaatttgat tgaaatcggt gttccttgtg ataattccat
    12781 tattcattct cctttacttc tcatagctac attgttccta gtttcttttg ttttgtcaac
    12841 caacttgtcc atttttacag aaaacctttt ttccaaaaca actctttgat tttagagtat
    12901 aatattgtag aaaatatgaa tttatacaaa ggagccggtt gaatgaaccc tgcgattcga
    12961 ctagatcagc acatttatca aaaaaacgac ttttcccctt ttgcattaac agatatatta
    13021 acggaatatt ctaaagaaca ccagcaaatc tttttacatt tctggcaata tgactgcaca
    13081 gtcatcttag gtatgaagga tgttcgaact ccttttctaa gtgacgggat ccaaacactg
    13141 aaagatgcag gttacgctcc agtgatcaga aattcaggtg gattagggat cgtatcagat
    13201 gaaggaattt tgaatatttc tttgattttc ccccaaagtg acacaaaaaa aataaccata
    13261 gatgacgctt acgagcaaat gctcctattg actaaatacg cttttccaaa ggcaccgata
    13321 caagcattcg aaatcaccca ttcttattgt cctggaactt atgatttaag catccatggc
    13381 aaaaaatttg ccggtatcgc tcagcgtcgg atcaaaaatg ggatttctgt tatgatgtac
    13441 ttaagtgtca acggtaatca ggaaaaacgc gggcaagtca tgaagcgatt ttatcaatct
    13501 gctttaaaag aagactttgg tacaaatggc tatcctgcag tggatcctgc tacaatggca
    13561 acacttgaag aactgcttga ccaacccctt tttgtaaaag aagtcaaaga agcatttgca
    13621 aaagcttttg aaaagctgta ttctgcaagt gatttacagg attctaccga ctggctaaaa
    13681 aaagaaatcc agctggagca atggaacaac caaatccaga gaatggaaca acgtaataac
    13741 ataaaggagc tacattatga caacccctta taaatatcaa atgctgccta tggaaaaagt
    13801 ttttcgtgat ccagtccata actatatcca tgtccagcat caagtaatct tagatttgat
    13861 caattcgaaa gaagtccaac gtctacgacg cattaaacaa ttaggcactt cttctttcac
    13921 ttttcacgga gcagaacata gccgttttac tcattcatta ggcgtttatg agatttcaag
    13981 acgaatctgc gatatctttt ctcgaaattt ttcaaaagaa aaaataggca acggaggctg
    14041 ggatgatcag gaacggctgg tgactttatg tgcggcactg ttgcatgatg tcggtcatgg
    14101 tccttactct catacatttg aacatatctt ccatacgaat catgaagcca tcactgtaga
    14161 gatcatcact tctccagaaa cagaagtgta tcagattctt aaccgagtag aagaaggttt
    14221 tcctgaaaag gtagctagtg tgatccaaaa aacttacccc aacccacaag ttgtccaaat
    14281 gatttctagt cagatcgatg cagatcgaat ggactatttg cttcgggatg cttatttcac
    14341 tggaacagaa tatggaacat ttgatcttac ccgaatatta cgtgtgattc gtccttatga
    14401 aggcgggatc gctttttcca tgagtggcat gcatgcagtg gaagattata tcgttagccg
    14461 atatcaaatg tatgtgcaag tgtacttcca tcctgtttcc cgtggtatgg aagtaatttt
    14521 ggagcatctg cttcatcgcg cacacgagtt gtatcaagaa gatgccagca gttttgaact
    14581 ccattctcaa ttacttctgc catttcttga cggaagcttt actttagaag aatacctcaa
    14641 acttgatgat ggggtcttgg gcacttactt tactcaatgg tctgaatctg ctgatcctat
    14701 tttaaatgat ttagctaaac gttttttgaa tcgtaaacca ctgaaatctg ctactttttc
    14761 tggcaaccgg gattcaaaat tagtccaaga actcactttg ttagtagaaa aggtcggata
    14821 taatcctgtt tattacactg ctgtgaattc tagctacgat ctaccttatg atttttatcg
    14881 cccagaacaa ggcagacatc gcacacaaat cgagattttg cgaaatgacg gaactttgat
    14941 agaattatca caggttagtc agcttgttgc tgcattagcg ggacaagaac aaggagatga
    15001 gcgatttttc tttccaaaag aaatggttga tccgtccttg cgtgatcatt atgatttatt
    15061 tgacgaaact tatcaagaat ttgccagcca tatccgtaat ggcgcattga ttgaaataaa
    15121 ttaaaatata ttggaggaaa ttatgtctat caaactagtc gctatcgaca tcgatggtac
    15181 tttattaaat gagaaaagag aagtaacaaa agaagtaaaa gaagcgattg ccgctgctgt
    15241 tgcaaaaggt gtttcgatcg ttttatgcac tggccgtccc ttaccaggtg tccaggaaca
    15301 gttaaacgag ttgaaccttt ttcaagataa tgattacgtt attacttata acggggcact
    15361 cgtacaacaa acgaaatcag gtaaaatcat cgcccgtcac ggactgacac atgaagattt
    15421 cctagagatc gaagtgatgg cacgccgtgt cggcagtcat ctccattcga tcgatgacaa
    15481 agcgatctac acgcctaatc gagatatcag cgcttatacg atccacgaag caagtttagt
    15541 caagatgccg ctaaaatatc gaacacctga agaaatgaca ccagacatga acatcgtcaa
    15601 aatgatgatg atcgacgaac cagaaatttt agatgcagca attgcgcgtt taccgcaaac
    15661 tttccgtgat aaatacacaa cggtcaaaag tgcgccctat tacttcgaag tactaaataa
    15721 agaagcaagt aaaggtgcgg cagttgcgaa tctcgcacag caccttggta ttgatcaaga
    15781 tgaaatcatg gctatcggag ataacgagaa cgacttatcc atgattgaat atgctggtct
    15841 aggagtagca atgggaaatg ccgtttctct tgtcaaagaa gcagcaaatg tcatcacttc
    15901 atcaaacgac gagcacggtg tagctgaagc aatcaa
//
LOCUS       DACKAC010000064        15889 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.065, whole genome shotgun sequence.
ACCESSION   DACKAC010000064 DACKAC010000000
VERSION     DACKAC010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.065"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(330..530)
                     /locus_tag="I0B46_002137"
     CDS             complement(330..530)
                     /locus_tag="I0B46_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAR2084409.1"
                     /translation="MKINYTVDLVSFSLVINFCLFSLRLFIEGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(835..2067)
                     /locus_tag="I0B46_002138"
     CDS             complement(835..2067)
                     /locus_tag="I0B46_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAR2084410.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSAAGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVIDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            2324..2893
                     /locus_tag="I0B46_002139"
     CDS             2324..2893
                     /locus_tag="I0B46_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAR2084411.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(3071..3511)
                     /locus_tag="I0B46_002140"
     CDS             complement(3071..3511)
                     /locus_tag="I0B46_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAR2084412.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLSGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            3669..4433
                     /gene="map"
                     /locus_tag="I0B46_002141"
     CDS             3669..4433
                     /gene="map"
                     /locus_tag="I0B46_002141"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAR2084413.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            4465..5388
                     /locus_tag="I0B46_002142"
     CDS             4465..5388
                     /locus_tag="I0B46_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAR2084414.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            5464..6603
                     /locus_tag="I0B46_002143"
     CDS             5464..6603
                     /locus_tag="I0B46_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAR2084415.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            6596..7396
                     /locus_tag="I0B46_002144"
     CDS             6596..7396
                     /locus_tag="I0B46_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAR2084416.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            7396..8223
                     /locus_tag="I0B46_002145"
     CDS             7396..8223
                     /locus_tag="I0B46_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAR2084417.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            8201..8935
                     /locus_tag="I0B46_002146"
     CDS             8201..8935
                     /locus_tag="I0B46_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAR2084418.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            9035..9901
                     /gene="rfbA"
                     /locus_tag="I0B46_002147"
     CDS             9035..9901
                     /gene="rfbA"
                     /locus_tag="I0B46_002147"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAR2084419.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9915..10487
                     /gene="rfbC"
                     /locus_tag="I0B46_002148"
     CDS             9915..10487
                     /gene="rfbC"
                     /locus_tag="I0B46_002148"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAR2084420.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            10509..11537
                     /gene="rfbB"
                     /locus_tag="I0B46_002149"
     CDS             10509..11537
                     /gene="rfbB"
                     /locus_tag="I0B46_002149"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAR2084421.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            11635..12486
                     /gene="rfbD"
                     /locus_tag="I0B46_002150"
     CDS             11635..12486
                     /gene="rfbD"
                     /locus_tag="I0B46_002150"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAR2084422.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            12520..14553
                     /locus_tag="I0B46_002151"
     CDS             12520..14553
                     /locus_tag="I0B46_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084423.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPAKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKIDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKEIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            14597..15877
                     /locus_tag="I0B46_002152"
     CDS             14597..15877
                     /locus_tag="I0B46_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAR2084424.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIATLLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYGEQPDSAHSSRIQRYH"
BASE COUNT         4883 a         2741 c         3290 g         4975 t
ORIGIN      
        1 ccatacttta taaaaatctt tgataaatct attcgaaggg tcaaatttaa accataattg
       61 tgtttttcct tccatcagca taaaagttca cctcaaaaag agtatacgaa cgaatgttct
      121 ttttgtgaag gcggaattta atatactgaa taatagaaaa caagtacttg tgccaaatta
      181 tgtgccaaaa aaattcgaat tcaacaaaat gtaaacaaat agaaaacctt ctatattaag
      241 atttcctgca ataacaaaca caacagaaga cgtgtaatag ttagtcagga acgtacaaat
      301 aacccctgta tcctttgcgg tacagggggt tattttgtat aagtagacat tgtagtttct
      361 aaactgctat gtcctaaacg tttcgatacg ctaagtatat ttactccttg ataaagtaaa
      421 atagatgcat gcgtatgtct tagtccctca ataaacagtc gaagagaaaa taggcaaaaa
      481 ttgattacta aagaaaaact tactaggtcg acggtgtagt tgattttcat acttcaacag
      541 attcgaatta aacgatttcg attggcttat accagtccta tccgacgaaa gatatatcgt
      601 tcttaactct agcggtataa cttgaagctt tacgtaaagc caatgggaat gaactggaga
      661 tgacttttta ttatttttag taatagctga tgggttaata cttacaactt tccttttcaa
      721 agtaccatat aaaaaaacgg tggggttgtt tagcgaaata aaggatctaa gccaatagct
      781 tagatccttt attctatgct ttatattatt ttttgcatgt gtgcgaaagt atactcaagc
      841 ccaatcgcca tttcggaaaa tcggtactgt agagccatct tcacggattc cgtcgatatc
      901 catcttgtca gatcctacca tgaaatctac gtgggtttga cttctattca atccagcttc
      961 tgctaattct tcatcggaca tttctgtacc gcctttcaca ctgaaggcat aagcagaacc
     1021 tagtgctaag tgattcgatg cgttttcatc aaataatgta ttgaaaaaga tgatgccaga
     1081 ttgagaaata ggtgaagggt caggtacaag tgcgacttct cctaaacgac gcgcaccttc
     1141 atctgtatca agcagttttg ctaagacatc ttctccttgt tcggcagaaa aatcaatgac
     1201 ttttccatct ttaaaagtaa atttcatacc cgagatgatg gtgccagcat aactcagtgg
     1261 ctttgtactt gaaatgtaac catctactcg atgactgtca ggtgctgtga acacttcttc
     1321 cgtcggcata tttgccatga atttttctcc acgagcatta tagcttccag cgccttccca
     1381 aagatggttt ttaggcaatc cgataatgat atctgtaccg ggagcagtat agtgcagggc
     1441 agaaaattgt tctcgattca attcttctgc tttttttgct aatttttcat catgcttctt
     1501 ccatgctaag acagggtctt cttcatagac acgagtcgtt ttgaaaatct gatcccataa
     1561 tgcctcaact tgtttttctt ctggcagttc tgggaagact tttgctgccc attgtttacc
     1621 agccgcagcc acgacagtcc aactgacttt atttgcttga gttgccttgc gtaaattcat
     1681 tagtgctttg ccagccgctg attgataaga cgctactcga tggctatcta caccagcaaa
     1741 tgcatcggga tcggcagaaa caacactgat cctgcttgct cctttttcaa gccattcatc
     1801 cgcttgatcg attttagatt gtggtacatt ctcaatacga tccgttgcag catgtaaaag
     1861 aaattctctt tggatctggt cgtcagtcca ttgaacgata acctcagcgg ctcctagttt
     1921 ataagcttct tgtgtgatca atcgagcaag gggtgcctgt tccacgctga tctgtaaaac
     1981 aactgtgtgt cctttttctg tggctacgcc agtttctgca attaagcgtg catatttttt
     2041 tagaagttca ttaaaatcag ataacataat aaaaattcct cctcaccatt ttttctggta
     2101 actattagca tgataccatt taattatgat tcaaacaatg aggcctttaa ataaaaaaaa
     2161 gaggttattt ttttaaggtt aagtttcatg atatatgtta ctacttgcta tttcagcttg
     2221 tgaagaaaat caaaaactaa ttttgcatat caaaaaaaac atgttacaat gaaattaatc
     2281 aatggtggaa gcgttataaa aaaattaagg agattaaaaa aatatggcaa gaaaaaagac
     2341 gattactaga gaacagattt tggcagctgc ttatgaagta gctgccaccg atggatttac
     2401 acgatttact gcgagaaaca tcgcgaacaa aatgaattgt tccacgcaac cgatttatct
     2461 tgagtttaaa aatatggatg acttgagaca agctcttttt gagcagatct atgatcattt
     2521 acaatatgaa gtgttcactg ttgagcatac agggaatacg attatcgatc ttgcttttaa
     2581 ttatatccat tttgcaagaa gagagaagaa gttgtatcgt tcactgtact tagaagaata
     2641 tggtggagga aaagagatgc agaatttctc ttatacttac tttgtgaata cagtgaaacg
     2701 cgatccagct tatgtgaatc tttcagaaga gcagatcgaa tctttgtata atgggacatg
     2761 gatcatagct actgggatcg cagctttgat gtcttcaagc atcatccatc caactgatga
     2821 acagattgaa aagatgatac aagacagtat agatgctatc ttgaaattca aagaaccaat
     2881 agaaatcgat tgaatatact caaaagttcc acaaatcagt agataacaag agatgtgtca
     2941 aaataaggaa aagttcgttt ttcttacagc gtatgcattt ttaactcccg aagaactctt
     3001 ccatgaatgc aatgaataaa agagccgtac agattgaata aatctggatg gctcttttat
     3061 tgcaagtata ttaaattttt gcgactagtt ctttagcgaa agcttctaaa ttttcgatat
     3121 cttcttcttc agcattcaga tccactttaa cattttcggc tccttttgat gcgcctgttt
     3181 ttgcaaaagc agcatcgaaa tcatccaccg atttgcagaa ttcatcgtaa aaagtatcac
     3241 cagatccgca gacaccgtaa atttttcccg atagatccaa ttcttgcaag tcttcataaa
     3301 agtctacgat ctcatcaggc agatcgccat catcgtatgt ataagtagct actacgcaaa
     3361 tatctgcctc ctcgaattca tctgcatcta cttgggtaca ttcgttgatt tcgacttcga
     3421 tatccagatt ttcaaatgct tctgcaacaa tatcggcgat ttcttcagtg tttcctgtca
     3481 tgctagcata tacgattttt gctaaagcca tgatgtgcct cctcgtttat aattatttaa
     3541 caattatgaa ttataccaaa acatcattga aaagaaaata aaacctagag acacctgggg
     3601 aatgtcattt ggctaaatgt atgccgctat gttaaaatga gagggatttg taaaaaggag
     3661 acgaaaatat gattacttta aaatcagcac gtgaaattga aatgatggca gaatctggag
     3721 ctttattagc agatgtacat aaaaatctgc gtgattttat aaaaccagga atcacaagtt
     3781 gggatattga ggtatttgtc agaaactata tcgagagtca tggcggaatt gctgcgcaaa
     3841 ttggctttga agggtatgaa tatgcgactt gtatcagtat aaatgatgaa atttgtcatg
     3901 gattccctcg taaaaagcct ttgaaaaacg gtgatttggt aaaagtcgat atgtgtatcg
     3961 atttgaaagg cggcatttct gattcttgtt gggcctatgt agttggagaa tctacacctg
     4021 agatcgaccg attgatggaa gtaacgaaga aagctttgta catcggtatt gagcaagcac
     4081 aagtagggaa tcggattggt gacatcggtc atgctatcca aacttatgta gaaggcgaaa
     4141 atctagcgat cgttcgtgat tttattggtc atggcgttgg tcctacgatt catgaagaac
     4201 cagcagttcc tcattacgga gaagctggaa aagggttgcg gttgaaagaa ggcatggtta
     4261 tcacgatcga acctatggtt aataccggaa cttggaaaat gaaaatggat cctaacggct
     4321 ggactgcgta tacaagagac ggcggacttt cttgccaata tgaacataca ttggctatta
     4381 ctaaagatgg tccaagaatt ttgacatcac aaggagaaga aggcacttat tgatctatcc
     4441 agtcaatagg tacgggagag gtacatgaaa gtttggaaca aaataaaaca aaatgaaaat
     4501 ctgatgcgct ttattgagac gactcaaaag cgcatcactg attcagaaat gagtacgacc
     4561 tctgtcgtcg ttgcttatta tttactcttg tctttatttc cattgattat tgcttttggt
     4621 aatattttac cgtttctcca catcgatcaa gagactgtac tgacctattt acgagaagtg
     4681 attcctgaga ccgtttacca gtttatcggt cctgcaatca aagacctttt gacacaaagt
     4741 tcaggaggct tgttatccgt ttctgcatta gcggctttat ggtctgcaag tcaaagtatc
     4801 aatgctttgc aaattgcgat gaataaagcg tatggcgtag aaaatagaaa aaattttttg
     4861 atcgtccgtt tcttctcact aattgtcatt gtcttgttca tgattgctat tagtggggta
     4921 actatcgttc ttgggttggg acagctgatt ttggatgcta ttcagccaat cgtacaaata
     4981 cccgtgaact ttatcgatca gttccaaact ttgaagtggc caatcacatt ggtagcttta
     5041 tttgtcatca tgttcttgat ttatttaatt gtccctaatg ctcagttgaa gttgaagacg
     5101 atcattccgg gcacgatctt cgctactgtc ggctggatgc tgttatcaca agtgtttagt
     5161 atctatgcta ggtattttgc aacgaggatc agcggttatc agatcatagg aagttttatt
     5221 gtactcatgc tttggcttaa ttttgctgct acgatcatca ttttgggcgg aatcatcaat
     5281 gcagttgttc aagaatacgt gacgggtcat caaatcaaaa aacgtcgaga tccaagccga
     5341 aaatggttca ataaattgaa aactaaattt tctcataaaa aaaagtagaa acaagagttc
     5401 ttattgtaaa tttatatcag taatttataa taattatgac tcttaattaa agtaggtgcc
     5461 catatgcttg tttcttttag tatggttata agattttttc tgacaatttt attatcactg
     5521 atattaacac ctgtattcaa aatcatttca gtacaaacag gtatggtcga caaaccaaat
     5581 gaaagaagaa taaataaagt tccaatgcct tcagcgggcg gactgcctat ctttatttca
     5641 tttgttatat cgactttatt cttattccgt aaaattatcc ctcaatcgta tattgtaccg
     5701 atcttgattg cttctttagt aatcatagtc acaggagtgc tcgatgataa atatgagctg
     5761 acacctaaac aaaaaagtat agggatcttg attgcttctc ttattattta ttttgtagca
     5821 gatatacgta ttgattcctt cacgcttcct tttattggat atatccaact aggctggctt
     5881 agttttcccg taacgatttt ttggatcttc ggtatcacga atgcggtcaa tctgattgac
     5941 ggtttggatg ggctggcggc agggatttca ttgatcggtt taattacgat aggaatcatt
     6001 ggatattttt tcctccatgc ttctacagtg tatattccag ttgttatttt ctgtcttgta
     6061 gcaagtatta tcggtttttt tccttacaat ttttatccgg caaagattta tttaggcgat
     6121 acaggagctc tattcctagg gtttatgatg gccgtccttt cactacaagg gctgaagaac
     6181 gtgacttttg tttcatcgat ttctttgctg atcgtcatgg gagtgccggt aacagacacg
     6241 ttttttgcta tcattaggag aaaagcaaac cgtgtttctt tttctacagc agataaaaaa
     6301 catcttcatc accgactaat cagtttaggc tttactcata aaggtgctgt cctgacgatt
     6361 tatgcgatgg ctttgatgtt ttctttcaca gctatggtca tgaattatac tggaagaatc
     6421 ggaacgatca tattgattat tgccatgttg tttgctgcaa ttttattatt tgaattgatt
     6481 ggtctgatca atgagaaaca tcagtttatt ttaaaaacat tgcgattttt agggaataaa
     6541 gagtaccgta cgcaagtaaa acagaaatat ttgaagaaat ggaaccgtca tatagatgaa
     6601 taacaaatta aaccagccga tccttattag tattgtgacg gcaaacagtg ataaaatttt
     6661 tcggacacta gataatttta tttcaactgt aaaagatgaa acagcagtaa aaatccgaat
     6721 ttttgacaac aattcacttc ccgagtacaa aaaaagacta acagagtata ggaaatatcc
     6781 ctacatcgat atctattttc atgatgaaaa ttccggattt ggttatggac ataatcacaa
     6841 tctgatagct agcgattctc gctatggcat tatttgtaac ccagatattt tagtggatga
     6901 agagacagtt acagcattgg tcttgttatt gaaagaacat ccggaatgtg cgatgttagc
     6961 acctaagatt cttaatgaag atggaacaac tcagcacctc atccgtgagc agctggctgt
     7021 ttttgattac gttttgcgct ttgtgccttt caaatgggtc aaaaaaaagt ttgacaaacg
     7081 actggctgtg tttgaatgcc gaggattgcc tactgatgct aatagttatg tacggatgat
     7141 ctcaggtagt tttatggtag ctgatattga taaatttaaa gagataaagg ggtttgatga
     7201 gcgtttcttc atgtactttg aagacaacga cttgtgtttg agttttgaaa aagcaggtta
     7261 caagttattg tacacaccct tttatgcagt tatccatttt tacgggaagg gtgcgcatcg
     7321 aaatgcaaaa ctgtttcgcg tgtttttgac ttctatgatg aaatttttca ataagtgggg
     7381 gtggcgtttt ttctgatgaa accagaaata ttagtagtcg ttgtgcttta tcagcaacta
     7441 ttcagtcaat ctccagctta tggaccatta agcaaagcac ttagcgaaaa gcaaattcag
     7501 cttattattt acgataatag tcctcaaagg cagcaaaatg agctgtttga aaaagaaaat
     7561 atcgtttatc atcatgatcc gcaaaatcct ggtttagcgg tggcttataa ttttgcgttg
     7621 tctcaagcag atgaaggaac acgttgtctc gtcactctgg atcaggatac gaggctagtc
     7681 gacgattatt ttgaactatt gcgtaaagta acctttacag atgaatgtgt tgctgcagtt
     7741 ccgatgattt tttctgggag ccgacaagtt tctcccctat attcagatcg ttatatcaat
     7801 cgtacagctc aagcagtaga agttgaaggc ccaacagctg aacgaatcat ggcaatcaat
     7861 tcaggtgttg cctggtctgt caaatttcta aaaaagattg gcgggtttaa tcctgctttt
     7921 tctttagatt ttttggatca ttggctgttt tggaaagtgg atcagttgga aaaaactgtg
     7981 gagatccttc ctgtacgaat ggaacatgat ctttcggttt tagactatga aaaagtttca
     8041 agtaatcgat accgttctat tttacaagca gaaaatcgat tttatcatga atatgatcaa
     8101 aggcatttat ccagtcaccg acgacaatta ttgttaagaa cagctaaaca gttcttgacc
     8161 gtcaaaaacc ggcatatctg gcggatgact ttgcgctcat atgttaagaa ttggaaggtg
     8221 tgaattcgtg cgttcggttt gtatagctac ctttaatgga ggcagatatt taagagagca
     8281 gttagatagt attttatctc agctttcagc agaagatgaa gtagtcattt ctgatgatgg
     8341 gtcagtagat gatacatggg atatattaac tgaatacgca gaaaaagata agcgaatcca
     8401 gcttttttcg gggcctcgtc aaggattgat cgctaatttt ggttatgcga tccagcagac
     8461 gaaaggagat ctaatctttt tggcagacca agatgatgtt tggttggaga caaaagtgaa
     8521 aaagatcact gactattttg agcagaatcc ggatcagcta gtagtagttt ctgatttaat
     8581 cattgtggac agcgagctgc aagagatcca tgattcgtat ttttcgtttc gaaattccaa
     8641 agaaggatgg cttaataatg tgataagaag tcactatatt ggtgctggta tggctttccg
     8701 gtcttcttta aaacccgata ttttgccgat cccgtcagaa gtgccgatgc atgatatgtg
     8761 gattggtttg ctgggaggaa agaagactgg gttcctaaga gaaccgttaa ctctgtaccg
     8821 aagacatgat ttaaacgcga gtgagattga aacacattct aaattacggc aaaaaatttc
     8881 ttggcgtgtt catctgatta cggcgttgat caaacgaaaa tggtttaaac gttgataaaa
     8941 gatgtttaaa tccttaatat ttccatgaca aatataacaa ttgatgagat aataacaaag
     9001 tgaaatttga aatcaaaaag gagagcagat atccatgaag ggaatcattc ttgctggggg
     9061 cagcggaaca cgtctgtacc ctttaacaaa ggcaacatcg aaacaattga tgccgattta
     9121 tgacaaacca atgatttatt atccaatgtc tactctgatg ttggcgggaa tcaaagaaat
     9181 tctgattatt tcaacgccac aagatacacc acgtttcaag gaattatttg gaaacggaaa
     9241 tgatttagga ctgcagatcg aatatgcagt acaagaaagt ccagatggat tggcacaagc
     9301 gtttatcatt ggagaagaat ttattggaga tgatagtgtc tgcttggtat taggggacaa
     9361 tatttactat ggtggcggtc tgtctaaaat gttgcaacga gcagcttcaa aagatacagg
     9421 cgcgacagta tttggctatc atgtcaatga tccagaacgc tttggtgtag tagaatttga
     9481 tgagaatatg caagctttgt cgattgaaga aaagcctgct cagccaaaat caaattacgc
     9541 tgtaactggc ctttattttt atgacaatga ggtaatttct atcgctaaag gtatcaaacc
     9601 ttcagaacgt ggagaactag agattacgga tgtcaataaa gcatatttgg ataaaggaaa
     9661 actatccgta gaacttatgg gacgcggatt tgcttggctt gatacaggaa cacatgaatc
     9721 attgcttgaa gcatctacct ttatcgaaac aattgaaaaa cgtcagaact taaaagtggc
     9781 ttgtttggaa gaaattgctt accgtatggg ctacatcgat aaggaacaat tgctggccct
     9841 tgcacagcca ttgaaaaaaa atcaatacgg tcaatattta ttgaatttgg ccgctgaata
     9901 aggagtcctt acagatgaaa gtaacagata caaaattaac agatgtcaaa atcgtagaaa
     9961 tggacgtctt tggcgatcat cgcggtttct tcacagaaag ttattcaaaa gcaaaatttg
    10021 cagaacatgg attgaaatat gactttgttc aagacaacca ttcactatca gctgaagctg
    10081 gtgtccttcg ggggcttcat ttccaaaaag gagaagctgc acagacaaaa ttgattcggg
    10141 tagtcactgg agcagtttta gatgtagtag tggatatccg caaaggtagc ccgacttatg
    10201 gtcaatggga cggatttatc ttatctgaac ataatcatcg acagttgctt gtacctaaag
    10261 gatatgccca tggattcgtt actttgacac caaatgtgaa cttcctttat aagtgtgata
    10321 attactataa tgcagaagca gatgggggca tcgcatttga tgatccagat ttagcgattg
    10381 attggccaat tgatccatca agagcgatta tgtcggaaaa agatcaccgt catccgacat
    10441 taaaagaatt tgaagcagaa aatccatttg tttacggaga aatctaagaa ttaaggagaa
    10501 attttcttat gaaaaacatc attgttaccg gaggtgccgg attcatcggc tctaatttcg
    10561 ttcattacgt ggtaaacaat catccagaag tgcatgtcac tgttttggat aaactgactt
    10621 atgcaggaaa caaagagaac ttggctggac ttcctagcga tcgcgtagaa ttggtcgtag
    10681 gagatgttgc agatgcagaa ttagtggacc gtctagtaaa agaagcggac gcagttgttc
    10741 attacgcggc agaatcccat aacgataatt cattaaaaga tcctttccca tttgtacaaa
    10801 cgaatatcat tggtacgtat acacttttag aagcttgccg caagtatgat gtacgctatc
    10861 atcacgtatc tactgacgag gtttacggcg atcttccatt acgtgaagac ctgccaggac
    10921 atggggaagg cgaaggagaa aaattcactg cggaaacacc atataatcct tcaagtccat
    10981 attcttctac aaaagctggt tcagatctgt tagtaaaagc atgggtgcgt tcttttggct
    11041 tacgtgcaac catttcaaac tgttcaaaca actatggtcc ttatcagcat attgaaaaat
    11101 ttattcctcg ccaagtaacg aatatcttaa gcggtattcg tccaaaatta tatggcgaag
    11161 gaaagaatgt tcgtgactgg atccatacga atgatcattc ttcagcagtg tggctgattt
    11221 tgacgaaagg cagaattggc gaaacctatc tgatcggtgc agacggtgaa gaagataata
    11281 agaccgtgat ggaattgatc ttagaaatga tgggtcaacc aaaagatgcg tacgaccatg
    11341 taaacgaccg tgcaggacac gacttgcgtt atgcaatcga ttcgacaaaa ttacgggaag
    11401 agttaggatg gaagcctgag tttacgaact tccgtgatgg gttggctgac acgatcaaat
    11461 ggtacgaaga acatgaagac tggtggaaaa aagaaaaaga ggccgttgaa gcagcatatg
    11521 ccaaaaatgg acaataatac agagaaaatg tcactgtatt aatggaaaaa cacatgtgag
    11581 gcaggcgctc gcctgtctct tttttaaaat aaaaatcaag gagcggataa tcatatgttt
    11641 ttattaactg gaggaaatgg gcaactaggt acagaattgc gccatttatt agatgaaaaa
    11701 aatctgcaat atgtttcaac agatgcaaaa gacttggata tcacagatgc cgagaaaaca
    11761 atggcttaca tcacagaatt aaaacctgat gttattttcc attgtgcagc ttacacagca
    11821 gtagataaag cagaagaaga agcaaaagaa ttagatgaga agatcaatgt tgatggtaca
    11881 agaaatgtag cacttgcagc aaaagctgtg aatgctcaat tcgtctatat cagtacagac
    11941 tatgttttcg atggaaaaaa caaaaatgta gaatacgaag tagacgacca aacgaatcca
    12001 ctaaatgaat atggtcgtac gaaattatta ggcgaaaaag cagtagaaga gattttagaa
    12061 gactattata tcatacgaac ttcttgggtt ttcgggatct atggacacaa tttcgtcttc
    12121 acgatgcaac gcctagcgga aacccgcgac aaactgactg tagtaaatga ccagtttggg
    12181 cgaccaactt ggacacgtac tttggcagaa tttatggtat atgtcatcga agaaaaagca
    12241 ccatttggta tttatcattt gtcgaacgaa aatagctgtt cttggtatga attcgcaaaa
    12301 gaaatattga aagatacaga tgtagaagtt gcacctgtta cgtcagaaga atttccgcaa
    12361 aaagcaacac gtccgcagta ttcagtcatg agcctgaaaa agacagaaga gttaggattt
    12421 gtcataccaa cttggcaaga agcacttgcg caaatgctgg aagctgccaa aaaaatgaat
    12481 aagtaatttc cttttatcaa atagaatttg gagcatgata tggaaaaaca aacaagaagt
    12541 gcacgacgaa aagaaaagcc aaaagcgaac caggcagatt cgaatttggt gcttgacctg
    12601 tttctaaaac tccgctatct tattggcgtt ttagtcatcg tggtcatcgt tacttttaat
    12661 ttaaacggaa gttcacttgg tgtttgggat aaatatgtgt cgcaaagaga cgatgggaaa
    12721 aaaactgatg tgatttttgg acagaatcgg gaggtgcggt cagacgaatg gctggtgcag
    12781 acaccttttt acctctcaca agctgaagag gactttccgt tggtgaacga ggattattca
    12841 ttaaacggac aaaacatgat cattgcgtat aattctcctg caaaggatat tacggttatc
    12901 ggaaaaccat tcaactgggg attctttttc ctcggaagag atcgaggact ttccttttat
    12961 tgggcgatga aattagtcgt catggtcttg cttggattcg aagtactgat gatattaacg
    13021 aagagaaaaa aagctctatc gctaatcggt gcatttgcgc tgacattttc tccagcagtc
    13081 cagtggtggt tcatgcaaca tgtgggcgat ttgattttct ttactttggg actgatgact
    13141 gctttttatc attatttcta caatcatgat aaaaaatggc tacgaacgtt gatgatgctt
    13201 ttggtggtta ttttcggtat tgggtttatt ttagtgattt acccagccca tcaagtgatg
    13261 ctggcgtact tgctgatttt ttattttgtt ggcctgctta tttactatag caagaaagtt
    13321 tcatgggatc tgttggatgt agtgttgatt ggaggggcag ttttatttat tggcggagtc
    13381 atggttcatt tctggctaac gtcaaaagac gcattgttag cttctttgaa taccttatat
    13441 ccaggaaaac gggtaagtac tggaggaaag tggacgattg gcgaattttt ttctttcctt
    13501 acaaattgga aaattccttt tgaagatatc aactattcga ataactctga agtggccctg
    13561 ttctatcact tttttccaac tgtattccta gcaagtccat ttgtgttatt tggcaaaaaa
    13621 gatagcgaac aaaagttact gggacgtatt ttgatgattt tttgtctgtt ttctatcttt
    13681 tggattaccg ttgggttgcc aaaatggcta gcagaaatca ctctgctgtc ttatgttcct
    13741 ccattaagag ccatccttac ttttagtttt gcggcttgtt tattatcgat ctggtttatc
    13801 gcttatattt ggagagaaga tgttattcct atttggtata aattcatttt gttggcagta
    13861 aatgcatggt cttatttcta tgcgattacg cattcgaaaa tagataatta tttttcggat
    13921 tttgaaacca tcgcagttgc tgtgttggga acagtgattt tagcatttgc attatttggc
    13981 tggagacgct tgttttacct cgcttttgcc gccttagtta tcgtatctgg ctttttcgtc
    14041 aatccagtcg tagaaggtac aggagcgatt tttgaaaaaa cactggcaaa agaaatccaa
    14101 aaaatcgatc aacaagatcc tggtaaagtt tggttgtctg aagatgaatt atataacttc
    14161 accccaacat tgggagtgaa atccttcaat acagtacgct tttatccaga tatagctgca
    14221 tggaaggaaa tcgatccgaa agaagaatac gagaagtatt ataatcggta tgctcataca
    14281 agagctttta tcgctaagga agatacaaag ttcattttag atcgtccaga tatgttttcg
    14341 gtcacgttga attttgaaga cgcagaaaaa ttagggatcg actatgtgat cagtaaacga
    14401 ccattaactg attacaacca attatatgaa gcgcagttca cgcagctata tggaccggat
    14461 aaagacggtt atatgatttt taaagtagaa tatccggatt atccagatgt accacaaaca
    14521 caaactcagg aaaatcagct agtcgtacag taataggcta gctgagaata atcctgaaat
    14581 attagtagaa aggagagtgg agataattca agcaatcgta caaaaatttg tcaacaaaat
    14641 gagtgtcggt ttcttgcttt tgttgctgac gctagtcttt tttgtttgga cagcagggtt
    14701 aggttttcca gcaactttgt ttatatataa caatagttca ttacttatcg cttttagtgt
    14761 ggctattttg ctcattttaa atgtttataa agcaaaaggc gtcgattttc tttatctagg
    14821 tgttgcgctt gcccttttca ttttcttttc ttactttcgg gaattaagaa atgagtcttt
    14881 atttggagat ttactgtttc ctttgattat tttattttgt ttagtaatta aatggatcga
    14941 gtggacaaaa tgggatcgcg gtatttatct gattgtcttt acggtcttcc tcggaatcac
    15001 gttatatcgt gtatttacag agatccaagt accagaagga caaagcatct gggctccagg
    15061 caattcgctt tctgatattt ggatcaatac gaatacaatc ggaagttctt tgatgactct
    15121 tgctttattg atcagtggat ttgccagttc ttttgaacga tggtatgtac gaatcatcgg
    15181 tgcagtagca gttgttgcag cttttctcgg gatttgggtc tgccaatcta gaggtgcatt
    15241 agtcgcgttg attgtttttg cgatattgga tcttttacct aaatcgttga tgcgtgtcat
    15301 tcgtgcgcca tttattgctt acacggttac gattctttta gctttgccga tttcttatct
    15361 ggcggctgtc tctgaaaaag tcaatttgtt taccggtcga gaagatattt ggcataaatt
    15421 ctatcagacg ctaggagaaa aatctgaaca agttttacta ggaatgaaaa catttatctt
    15481 ccaacgagga aaccaatttt taggaaacca caatagttat aattctattt tgaatatcta
    15541 tggattgata ggatttggga ttgctacttt gctgcttatc ctttttattg gtcgcttaac
    15601 gttgaaagca gatttatcca acgggcagat gacttttatc tgggcctttt ttgcggtgat
    15661 gatgcagtca tttatggagg atacattgac ttcataccca tgggttccga ttatttatat
    15721 cctgttggct atggccgttc atcgatatga tgaaccagaa gttgcggagg aattctatgt
    15781 tatggaagag gcatatgacg atatgactta tgatgataca tcttatgggg aacaaccaga
    15841 tagcgctcat tcttccagga tccaacgcta tcattgatag gcacaaaat
//
LOCUS       DACKAC010000065        15860 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.066, whole genome shotgun sequence.
ACCESSION   DACKAC010000065 DACKAC010000000
VERSION     DACKAC010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.066"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(12..482)
                     /locus_tag="I0B46_002153"
     CDS             complement(12..482)
                     /locus_tag="I0B46_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAR2084425.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(641..1099)
                     /locus_tag="I0B46_002154"
     CDS             complement(641..1099)
                     /locus_tag="I0B46_002154"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAR2084426.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1270..2415)
                     /locus_tag="I0B46_002155"
     CDS             complement(1270..2415)
                     /locus_tag="I0B46_002155"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAR2084427.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2428..3345)
                     /locus_tag="I0B46_002156"
     CDS             complement(2428..3345)
                     /locus_tag="I0B46_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAR2084428.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPNGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3773..4336
                     /gene="efp"
                     /locus_tag="I0B46_002157"
     CDS             3773..4336
                     /gene="efp"
                     /locus_tag="I0B46_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAR2084429.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            4822..6081
                     /gene="lysA"
                     /locus_tag="I0B46_002158"
     CDS             4822..6081
                     /gene="lysA"
                     /locus_tag="I0B46_002158"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAR2084430.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            6068..6868
                     /gene="dapF"
                     /locus_tag="I0B46_002159"
     CDS             6068..6868
                     /gene="dapF"
                     /locus_tag="I0B46_002159"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAR2084431.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            6871..7752
                     /locus_tag="I0B46_002160"
     CDS             6871..7752
                     /locus_tag="I0B46_002160"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAR2084432.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            7749..8465
                     /locus_tag="I0B46_002161"
     CDS             7749..8465
                     /locus_tag="I0B46_002161"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAR2084433.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            8471..9166
                     /gene="dapD"
                     /locus_tag="I0B46_002162"
     CDS             8471..9166
                     /gene="dapD"
                     /locus_tag="I0B46_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAR2084434.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            9182..10183
                     /locus_tag="I0B46_002163"
     CDS             9182..10183
                     /locus_tag="I0B46_002163"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAR2084435.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            10180..11541
                     /locus_tag="I0B46_002164"
     CDS             10180..11541
                     /locus_tag="I0B46_002164"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAR2084436.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            11501..12652
                     /locus_tag="I0B46_002165"
     CDS             11501..12652
                     /locus_tag="I0B46_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAR2084437.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            12627..13796
                     /locus_tag="I0B46_002166"
     CDS             12627..13796
                     /locus_tag="I0B46_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAR2084438.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            13971..15350
                     /locus_tag="I0B46_002167"
     CDS             13971..15350
                     /locus_tag="I0B46_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAR2084439.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            15631..15860
                     /locus_tag="I0B46_002168"
                     /pseudo
     CDS             15631..15860
                     /locus_tag="I0B46_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         5186 a         2798 c         3378 g         4498 t
ORIGIN      
        1 tttttattaa gttaaagaac ggattcaatt gcagcggtca ttttcaatgg agaagtttgt
       61 ggtgcaaatc gttcgattac tttgccattt ctatcaatca aaaatttagt aaaattccat
      121 tttattgctt tagtgacagt accaggagct tcatttttaa gataagtaaa aagaggatct
      181 gtattttctc catttacgtc aatcaattga tgcataggga aggtaacgcc ataagtggtt
      241 ttacaagctt ctgctgcttc agtcgctgaa tctagctctt gtttaaattg attggaagga
      301 aagcctaaga taaccagacc ttgtgtctga taagcttggt aaagttcttc taattgttta
      361 aattgaggtg ctagaccgca ttttgttgca gtgttaacaa tgattatcgg tttgcctgca
      421 tattttttca atgaatagac agtgccgtct tctaaagtag tttcaaaatt ataaatagac
      481 ataaagtttt ctcctttcta ttttatataa taaaaagagc gctaaactga aaactagcta
      541 tttgttttcc cgtttagaac tcttttcaga caataaatat ttaataaaat acgaagataa
      601 gatagctata ttgcttctca tctttacgtt cttttagaaa ctatgcttct acttcagacc
      661 attcatcgcg ttttgctaaa aattcacgag ccaaagcttg tgcaccttct agtgtatggc
      721 tggcagccca gccacattgt gtttcattgc ttgcaggtac ttcagtagct tcaagaacat
      781 ctttcattgt tgcttcaaga acatctaaaa tgtcttcgta gctttcatga ttcaatacag
      841 tcaagtaaaa gcctgtttgg catcccattg gagaccagtc tacgatgtaa tctgcgtgat
      901 tgcggattag ttcggcagtc aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt
      961 ctttatttgg ttgtttaaag cggacgtcgt atttaatgat gacatcgcca tgcgcacccg
     1021 tttttcgatc agctacacgt acataaggtg ctttgacttt tgtatggtca agattaaaac
     1081 tttcgacatt cattctcatt tgtaagactc ctatctgttt gttcattcgt tttgatgctt
     1141 ttcactaaag aattatttag tgaaatttac aaaaacgtct ttctacttga ttctaacact
     1201 tatcgtttcc aaggtgtagc aatctgaaat attttttaga tttttctgct aagtcgttta
     1261 ttgggacttc tagttcttga ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg
     1321 accatactca attcctacag aaagacggat caagccgtcc tttatgccag cctcttctcg
     1381 tttttctttt ggaatagacg catgagtcat gacagaagga atttccacta aactttctac
     1441 gccacctaaa ctttctgcta aagtaaatag ttggagtgat tcaacaaatg gaatagcaga
     1501 ttcttcattt tttaaagtaa aagaaatcat tccgctaaag ccgctcatct gctgcttagc
     1561 aactccatgg agttcatgat cgggtaatcc tggatagtag accttttcta ctgctggatg
     1621 agaaaataga aaatcagcta ccacaaaagc atttttttga tgttcctcca tacgaacact
     1681 tagtgtcttg attccgcgct gaagtaacca gctatcttgg ggaccaagta cggctccgat
     1741 agcattttgg taaaatccga tctgatcggc tagatcttta tggttagaag tcactaaacc
     1801 agctactaca tcgctgtgtc cgcctagata tttggtaccg ctatggacaa cgatatcaga
     1861 accgagatct aaaggtcttt gaaaataagg agtagcaaat gtattgtcag caataactat
     1921 caatccatgt tgatgagcca gagtggcact tttttctaaa tcagtaatct ttagaagcgg
     1981 attgctaggt gtttcaagat aaagtgcttt tgtattgggt ttgatggatt gttctatttt
     2041 atcaagatta ctcgtatcta tgatcgtgta ctcgagacca tttttagtaa gaactttgtc
     2101 aaatagacgg aatgttcctc catagacatc atctcctaat aagatatgat cgccagcttg
     2161 aaaaagagaa aaaacggcat gaatcccaga aagaccagaa gaaaaagcga atcctcgaac
     2221 accgccttcc aaatcagcaa tcagttcttc caatgcaaaa cgagtaggat taccagatct
     2281 tgaatattca tattgtttag gctggccgac gccatcttgc cgataagtgg atgtttgata
     2341 aataggtaca ctgacagctc ctgttgtggg atcttcactg atgcctccgt ggatcaattt
     2401 tgtttgaatg tgcatgtttc tttcctccta taagtaaatg ttttttgata aatagcggtc
     2461 tgctgcgtct ggaaaaatcg taacgacacg atgaccaggt ggtaaacgac gaatttctct
     2521 taatgctgct gcaaaagccg caccactcga acttccaact aatagtcctt gttctcgtgc
     2581 taactgccta gtatagttaa acccttctac atcagagatt gtctcgatct gattgattga
     2641 aaggggagaa agaaacggcg gaatgaattc tacaccgata ccctcaattt cgtgaggggc
     2701 tggatcacca ccatttaaca cagagccctc tggttctaca ccgattattc gaatgtcggg
     2761 atatttttct ttcaaaaagg tagaagttcc agcaaaggtt ccaccactcc caattcctgc
     2821 tacaaaacta tggatatttt ctttgatttc atgaaagatt tctggcccta acgtatgata
     2881 gtaagcagca ggattatctt tattttcaaa ttgaagaggc agatagctgt ttgatatctc
     2941 ctctgcaagt tttttacttt tgctgatggc accgatgatt ccttgctcac taggagaatg
     3001 gatgatcttt gctcccaatg ctttcatcaa ttgttgtttt tctaagctaa acttctctgg
     3061 aacaacaaaa accgttctta atttgtatgt gagtgcggcc agagcgatcc caattcctgt
     3121 atttccggca gttggttcga tgatcgtcgt tttttcattg atttttcctt gggcaaatcc
     3181 ttctttgatc aaataactgc ccaatctatc tttgatactt cctccaggat ttaaatattc
     3241 tagttttgca taaatagcag aaccattagg catagaatag tgctgattgt caaagcgata
     3301 aagcggtgtg gaaccaatag cttctgtaat tgtcgagata atcatgtggg cctcctaaaa
     3361 aaagataata aaaaaaggag ctgactatta aatcagctcc ttgaaagaca tagagaaaaa
     3421 ttgagtagat aaaaagataa gttacctggt actcattcta tgaagggcaa ttaatagaca
     3481 tgacaaatag tatgctgctt ttatgcacca tacaacagca acgattcaat tgtttttggt
     3541 tgctgactgt catgtgtatc cctccaagta aaatttttat taacgatagc atgaaaaaaa
     3601 ataaaagtca aaggcttgaa agaaaaaaat atttcgatag catcgtttac ccgtgtttat
     3661 ttttctgaca aataggcatg agaccttaaa agatagagaa tactctagtt ttctggccgg
     3721 ttgtctgata caataaagtt gattatgata aagaaaagaa ggagcaaaaa taatgatctc
     3781 agcaagtgat ttaagagccg gtatgacttt cgtacaagac ggcaaactga ttaaagttct
     3841 ggatgcaagc caccataaac ctggtaaagg aaatacagtt atgcgtatga aactgaaaga
     3901 tgtccgttca ggtgcaacat acgacacaac gttcagacca gaagaaaaat ttgaaaaagc
     3961 acatatcgac acaaaacctg tacaatattt gtacacgatg gatgatacag ctttcttcat
     4021 ggatttggaa acatatgagc aatatgaaat tcctgtagaa acagtagaag aagaaatgaa
     4081 atatatccta gaaaacatgg aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt
     4141 tcaattacca acaactgtcg tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc
     4201 aacagtcact ggttcaggta agccagcgac aatggaaact ggcttagtag tcaatgttcc
     4261 tgacttcatc gaaacagatg aattgttgga aatcaataca gcagaaggtt cttatgtaaa
     4321 acgtgcgtca aaataattcg ctaacggatt gataagaatt tgaaaacaga gcttgacctc
     4381 ttgaaaggca gatcatcgga tctgcctttc aagaggtttt ttgtggaatc agcaaattga
     4441 aaaataaaaa agtatttgat tttatttata ttttgaaaga tgaatggcaa aagctagaga
     4501 aagaaaaaac tgcacttatt tatgaagaaa ttgaaatgat tcataaaatt gaaaatatcc
     4561 gaactattcc gaactatatt gaagcgaaaa tattttttca tcactaaata gtgttgaatt
     4621 tatgttttta tgtataaata gcttctaata gttcgggtat tttgttttta taaccatttt
     4681 tattcttatt tttaaaaaaa tatttcgaaa tatactagac aattcagaaa attttgttta
     4741 atattattcc aataaaattc aaatctttat caaatgaaat tccaataaaa acggaaaaaa
     4801 agtgaaaaga ggaatgaaaa aatgacagaa ttgataattg gcggagtcac agcaagcaca
     4861 ttggcagaaa agtataagac acctctgtat gtgtatgatg aagaaaagat gagacaaacg
     4921 atgctgtcat tcaagcgagg ctttgtatcg aaacgttttg atacaaaagt gctttacgcc
     4981 tctaaagcat tccaaacgat tgagatgctg catttagttc gtgaatatgg attcgggcta
     5041 gatgttgtca gtggtgggga aatctatacc gctttgcaag ctgattttcc tagagatact
     5101 atctacttcc atggtaacaa caagacgcca gaggaactga tctatgcact tgaaaatgat
     5161 ttgttgcata ttgttgccga taatttgatg gaagttgaat tattagcaga attaagccaa
     5221 cgctatttaa aaaaaatttg tatcatgctt cggttgaatg tcggagtaga agcacatact
     5281 catgaatata tcgtcactgc gcatattgat tcaaaattcg gcatgagcta tgaaagtaaa
     5341 gagtgccaac aagcccttca gttgataaaa gaaagcagct atctagagtt agaaggattt
     5401 catgctcata tcgggtcaca aatttttgat gttactgctt ggttagctga aatcgacaaa
     5461 ttgacgaatt acttaaccga ttttgaccaa cctctttcct tgaatcttgg cggagggttt
     5521 gggattcgtt atacacaaaa tgatcagccg atcccagtcg aagaggcact aaaaaaactg
     5581 attgaatata cggaagaagc tttggaaaaa cgaaaactga ccattcgtca attactgatc
     5641 gaaccgggta gaagtattgt tggagaagct ggtacaacac tatacacgat tggtttcatc
     5701 aaaaagacac cacataaaaa atattacttt gtagatggcg ggatgacaga taatatccgt
     5761 ccagctctgt atcaggcaga atacgattgt gatttagcag caaaactgtc ggaagaaaaa
     5821 acagagaaag tgacgattgc aggaaaaatg tgtgaatcag gcgatgtggt aattaaagaa
     5881 gcttatttgc cgaaagcaga atcgggtgat cttttagctg tttattccac gggagcttat
     5941 ggatactcga tgagcagcaa ctacaatcga gcgacacgac cagcagttgt ttttgtcaat
     6001 aatggccaat caagactgat tgttcgcaga caagcatttt ctgatctact aaggggggaa
     6061 gtttcctatg atccttcata aatataatgg ctgcggaaat gattttatct tattagatta
     6121 cgcagaagac acagattata gtcaattagc cgcttcttta tgcacaaaag aaaaatatga
     6181 tatagacgga ttgatcgccg taaaaatcga tccgttggaa atgatctatt acaacaaaga
     6241 tggcagccgc gcgccgatgt gcggaaatgg cattcgctgt tttgctcgat acgtaaatga
     6301 tcagaacatg atgaatgaac aggaattcaa cgtagagaca ttagcaggca caagaaaaat
     6361 tacggtgttg gatccctcac ctttttattg cagcgtagat atgggagtgc cagatgattc
     6421 tccggaaaaa atcaaagcta atcaaactta tccaataaaa aaccaggaaa tagagatcaa
     6481 tggtgaaaaa gtaacgatca ctagtttatt tttaggaacg attcataccg ttgtttttgt
     6541 tgaaaatgcg cgtgaggaag tctggagaaa aagaggtgaa ctgatctgtc atcatccttt
     6601 gttcaaagaa aaaaccaatg tcaactttgt agaagtagtc aatgaacaag aattgatcgt
     6661 ccgtacctat gaaagaggag taggttggac acttgcttgc ggtacaggtt gctgtgcctc
     6721 ttatgtggtt gctaaggaaa ctgggaaaat ttcagcagat caggtagttg ttcacttgga
     6781 acaaggagac ttgatgatct ctggtgagga tgcgatacaa atggctggcc cagcagttca
     6841 tgaatggaca agaatattgg aggaatgaag atgataaaag gttcgatcgt ggcgctgatc
     6901 acgccgatga atgaagaggg atcagtagat tatgctggtt tggaaaaatt gatccaattc
     6961 catttagatg aacaaacaga tggattatta gttttaggta caacaggaga atcttctacg
     7021 ctcacacaat ctgaggagga gcagatcctt caactgacag taaagaaagt ggctggccgt
     7081 gtgccggtga ttgcaggagc tggaacgaat aatacgaaag aaacgattga aaaagcgaag
     7141 cattttgctt ctttgggggc agacgcactt ttggtcatca ccccttacta caacaaaaca
     7201 agtgatgcag gactagctgc tcattttaca gcaattgcag aagcctcacc gattccgctt
     7261 attttgtata atgttccttc tagaacaggc atgtcgattc caatccatgt attagtaaat
     7321 ttagcagaac atcctaatat cattggtttg aaagaagcct caggagatat ggcttatgtg
     7381 atggatgccg ctcggttgat tggagaagaa ttcttccttt acagtggaaa tgatgatttg
     7441 atcctaccag taatgagtgt aggaggaagc ggtgtaataa gtgtatgggc gaatatccaa
     7501 cctaaaatag tccatgaatt agtaaaagat acgcaggacg gaagatggca gcaagcaaaa
     7561 gaaaagcaat taaatgcact tgaactgatc catgccttgt tcagtgagac caatcctatt
     7621 cctgtcaaag cagccatgtc attattagac ttaccttccg gacctctacg cctgccttta
     7681 gtttctcttt caaaagagaa aaagaaacaa ttagctcagc ttttgttaaa agaaaggacg
     7741 gttgaaaaat gaaaatcgga ctgattggca gtggacagat gggaaataga gtcgaagaag
     7801 ttgcacaaga aagaggagat acggtccttc ttttacggac ggcacctaaa gaagcaacta
     7861 cgcttgcttt tacggaactg ccagaactgc ttattgattt ttctcatcca gataatttag
     7921 agaaaatcct ttcgtatagc ttatcatatc aacttccgtt agtcattggc acgacaggtt
     7981 atacacaatc acagttccaa gaaataaaag aacatgccaa acatgtacct gtgatgtata
     8041 gtgctaattt ttcgttcggc atcatggtga tgaatcaact aatcaagcaa gcagctgcaa
     8101 tgttgcaagg ctggcagatc gaactgattg aaaaacatca tagtcgtaaa aaagatgcac
     8161 catctggtac tgcaaatatg ctgcttcaaa caatccaaga agtgcaaaag ctgcagcccg
     8221 tatacaactg gcaagggaaa aaaagagaag aaaaccaaat tggtgtacac agtattcgtg
     8281 caggttcttt acctggtgaa catgatgtat tgtttgctgc tactgatgaa atatttacga
     8341 tcaaacatga atctttttct aatcgaatct ttgctgcagg cgcagtagaa gcagcggact
     8401 ggttgaaaga tcagtcacca ggttattaca aattagaaga tttattaatg gacaaagggg
     8461 tgtaaaaaac gtggaagctc aagaaatcat ccgctacatt caaacggcat ctaaaaagac
     8521 gcctgtgaaa gtctatctga atgtaaccga gccaatcgaa tttaaagaaa gtaaagtttt
     8581 tggtgaagga agcagcttcg ttgtttttgg tgactatgag acgattgctc cggttttaga
     8641 agctgaatca gaaaaaatca ttgaaatcga aatcgaaact gctgcaagat acagcgcagt
     8701 tcctttgcta gatattaaaa aaattaatgc tcgtatcgaa ccaggtgcta ttatcagaga
     8761 tcaagtgtca atcggcaaca atgccgtgat catgatggga gcgattatca atattggtgc
     8821 agtaatcggt gaaaacacga tgattgatat gggtgctgtc ttaggtggac gagcgacagt
     8881 gggcaaaaac tgccatattg gtgcaggagc tgtcttagca ggtgtgatcg aaccagcatc
     8941 ggccaaaccg gttatcgtag aagacggcgt actggtaggt gcgaatgcag tgattgttga
     9001 aggtgtccat atcggaaaag atgcagtcgt ggcagcagga gcagtggttt tagaagatgt
     9061 ggcagcagaa actgttgtcg gtggaatacc tgcacgtgta ttaaaaataa gtgatgatca
     9121 gacaaaagaa aatacagcat tgattgctgc gttacgcgaa ttatagagga gatggagaaa
     9181 aatgcgcgaa atagatacct gtattgtggg ggcaaccgga ttagtcggcc aaacgatgct
     9241 caaagtgtta gaagaatatg attttccagt aggacgattg aaattattag cttcaaaacg
     9301 ttcagccggt aaagtttgtg tattcaagaa aaagaaacac actgtacagg aattgacaga
     9361 aacttcattt gaaggatatg atttagcgct attttctgca ggtgcttctg tatccaaaga
     9421 gtttgcgcca atcgcacgaa gtcagggact atttgtcata gataattcct cagcatggcg
     9481 ggaagattcg gatattccat taatcgttcc agaggtcaat ctggaagact atacattgaa
     9541 tcggctgatt gcaaatccaa attgttcaac cattcaagcg gtacttccgc ttaaagcttt
     9601 acaagaagcc tttggtatcc agcgagtcaa ttattcgacc tatcaagcag tttctggttc
     9661 cgggcaaaaa ggcattgaag atcttcatcg tacgcaaaat gacgaggaac cagttttgta
     9721 tccttataat attagccaaa cggtgattcc ggaaatcgat cagcctttgg aaaatgggta
     9781 tacaaaagaa gaacagaaaa tgataaatga gacccgtaag attctgcatc agcctgcttt
     9841 gccagtatca gcaacctgcg tacgtgtgcc aatcgaaaat ggacatggag tctctgtggc
     9901 agttcaactg caaaaagagt ttacgacgga agaaattaga gagtgtctca gccagtttcc
     9961 agggattcaa ttagaagatg atctagcagc acataaatat ccgacgagca ttcttgctag
    10021 aggaacagat atggtttatg ttgggaggat cagaaaagac acttctttag agaacggatt
    10081 gttgttctat acgactgccg ataacatacg caaaggagcc gctgcgaatg ccgtacagat
    10141 tgctgcagct ttaatgcaga aaatagcgga ggtggcaaga tgagtaatac gctgaaagta
    10201 gcgaaattcg gcggaacatc gatgtctcat gaagagcaat tcaaaaaagt ccgtacaatt
    10261 gttctttcag atcccaatcg ccaagtgatc gttgtttctg ctttaggcaa acgtacgtca
    10321 gatgatgaca aagtaacaga cttactttat ttgatttatg cttaccttca gcatcatgtt
    10381 gattggacac ccctttggaa aaggatttgt gatcgtttca ttcaagtaag agatgttctt
    10441 gccctgcagt tacctattga agatgaacta gcacgcatac agaaacaact ggaagacgaa
    10501 aagataactg aagattattt agtaagtaga ggtgaatatc tgactgctca actgatggct
    10561 gagtatcttg gttaccagtt tattgacgca aaagacctga ttttctttga ctatgatggg
    10621 aaaatcgatc aggaaaaaac agcacaggaa atcacaaaag catttaaaag atatccgaaa
    10681 atggttgttc cagggtttta cggggcgaac cctgcaggaa aagaaaaatt attgggccgt
    10741 gggggaagcg acattaccgg cgcgatactg gcgaagatgt tgcaagcgga aagttatgag
    10801 aattggacgg atgtttcagg aatcatgatg gctgatccgc gaatcatcga tcatcctaaa
    10861 atcatcgagg aaatctcttt tagagaactg agagagatgg cttatatggg ggctaacgtt
    10921 cttcatgaag cagctgtttt cccagttcaa gaggctgata tccccatcca aataaaaaat
    10981 accaatgatc ctttcgctca agggacaaaa atatcgaatc acgaaataga aaaggaaaat
    11041 ggactgacag gaatagctgg acgcaaggat tttctttcca tcactttatt caaacgtcat
    11101 atgtccgatg aaatcgggtt tatctggaaa gcaatgagca tttttgctaa acatgagata
    11161 tcaatcgaac atattccttc agggatcgac aatatcggag tgatcgtctc agcagaagcg
    11221 attgccgata agttatttct catcacgaaa gaattaaaag aaacacttgg cgtggaagaa
    11281 atcgtcgtga tcgaagactt agctttgatc tctgtcgttg ggggtccgca taaagaattg
    11341 attggattat ctgggaaagt gttatcgata ttgaacaaat tagaaattcg cacatcgatc
    11401 ttatcacaag gagcacagga gttgaatctg atcatcggtg taccgaataa tcaatatgaa
    11461 acagtagtga agggtatcta tgaagggatg gtgaatacgg atgctgaaag aatatcgaat
    11521 ggcactccat caaattcctg aaatcgggtt tcatgaattc aagacaaaaa aatatttata
    11581 tgaacaagta aaaaacagcg aggccgttat ccatgaaatc ggcgaaacag gactactcct
    11641 ttattttgat aatcagcaag aagaaacgat tgcttttcga acagatatcg atgcactgcc
    11701 agtcaaagaa gaaacgaatc tctcttttgc ttctaagcat gaaggttata tgcatgcatg
    11761 tggtcatgac ggacatatgg cgatgctttt agggttgact gactttataa ctaaacacat
    11821 tgaggatctt atttataatg tagtactgat ctatcagcca tcagaagaaa tttccggtgg
    11881 cgcacaaagc gttattcagt caggattgtt ggataaatat gaagtacaag caatatttgg
    11941 ctttcatcta tggcctggac tagctaaggg agaagttttc tctcgaccag gacctttgat
    12001 ggcacaaagc agtgaaacag acattatcat taaaggccag tctgcacata ttgcttctag
    12061 tgaaaaagga atcgatagtc tggaggcagc tgtacgattt ttgaagaatg tttatgattt
    12121 tgaccggcaa ttaccggcag agtggaatca tctaatgaaa tttggccaaa tccaaggtgg
    12181 aacgatccga aatgttttag ctggagaagt tattgtatcc ggaagtatcc gttcttacag
    12241 ttcagatatg caaaattatt tgaagagggg attagaccat ttagcagaac agtttcactc
    12301 tacagctact atttcttttc gctataatga cggctatcca gctgtaatca accatagagg
    12361 cttatatgca gcactgttag attcaaacaa actacatgaa ttagatgctc cagttcttca
    12421 agccgaagat tttggcatat atacgcaaca atatccatgt gtatttttct ttttaggtgt
    12481 cggtgatacc cctgctttac atgatccgac gttcgatttt gatatggatg ttttagaaaa
    12541 tggtgtagaa tggtacaaaa cgattctttt tacagaaaat cttcttgtaa aaatcgatag
    12601 cagttcaaaa gaggagcgag taaataatga tgaatttatc caatcgattt agtcctaggc
    12661 tttctagaat agaagtatca aaaatacgac aattcgatca gcaaatatcg tcgataccag
    12721 atgtgatcaa actcaccctg ggagaaccgg attttccaac tcctgaacat gtcaagcaag
    12781 caggaatagc agcgattgaa gaagactttt ctcactatac cggaatgaga ggattagagg
    12841 aactgcggga agcggcttgt atatttcagc aacagcgtta tggattaact tatgatccgc
    12901 aaacggaagt tttgacgaca gttggtgcaa cggaagcgat tgcctctgcg ttgttatctg
    12961 ttttggaaga aggagacaag gttcttattc cagcgcctgc ttattcagga tatcagccat
    13021 tagtggaact agctggtgct gaattgatcc cgatagatac ttctgacaca gggtttgtct
    13081 gtcagccgga acaatttgaa cgagcatttg aacaatatgg atcagctgtc aaagcagtca
    13141 ttttaaacta tcctaacaat cctactggga cgacattgtc tgcaaaacaa ttgaaagcaa
    13201 tcgctgaggt tttaaaaaaa tatccggtat ttgttataag tgatgaagta tatgctgaat
    13261 tgacttatag tggaacgcat atgtccatag ctacttatct tccagaacaa acaatcgtga
    13321 taagcgggtt atccaagtca catgcgatga ctggttggcg agtgggcttt atttttgctc
    13381 aaaaagccct tattgacgaa ctaattaaag tccatcaata tctggtgact gccgctacaa
    13441 cgatgtcgca aaaagctgcg gctgaagcgc taataaatgg ggtgaaagat agcgagaata
    13501 tgaaagagca atatcaaaca cgaagagact atctggtcca gcaattggcg cctttaggat
    13561 ttgaagtgac ccagccaaac ggtgcatttt acttgttttg caaactccca gctgccattc
    13621 aaactgactc ttggcagttt tgtgtagaat tagcggaaca ggcaaaagtt gcgtgtatcc
    13681 caggaattgc atttggccca gaaggagaag gttatatccg gatttcttat gctagcagca
    13741 tggaaaaatt acatgaggcc tgcaaacgga tcaaagactt tttaacagaa cagtgattca
    13801 tagattttat caaaaaaaca atcgttaatt ttgcggtata ttctgcaagg agacgattgt
    13861 tttttaaatt tagttcaaat gaacgcgttt cttttgattc ataactaatg taatgttaag
    13921 tttaaattgt ttagaatgat tttaaaagaa tattcaagga ggtttcatca atggaaaaaa
    13981 tcaaagttgt cattgtaggt gcatctcatg gagggcatca atcgatcttg gaactattga
    14041 cacgatacgg tgaaaatgta gatatcacgc tatttgaagc aggagactat atttccttca
    14101 tgtcatgtgg tatggagctg tatttagaag acaaggtaac agatgtaaat gatgtacgta
    14161 atttcagacc agaaaacttt ccgcaatcca atgttcatat tttaaataat cacgaagtaa
    14221 aaacgatcga tgccgataaa aaaacagtaa cggttactag cgcatcagat aacgaaacaa
    14281 aagaatatcc ttatgacaaa ttgattttga gttctggtgt caaaccaaac aatctgccag
    14341 taccaggagc agatctagaa aatgtctact tgatgcgtgg atacaattgg gcaaccaaaa
    14401 tcaaagaaaa attaaatgat ccggctatta aaaaaatcgc tgtcgtaggt gcgggttaca
    14461 ttgggatcga agcagcagaa gcaagccgaa aagctgggaa agaagtcgta ttgcttgatg
    14521 tgatcgatag acctttaggc acttatttag atgctgagat gacagatatt ttagaacaac
    14581 acctaaatga aaacggtatt gaagttttga caaatgcaaa aatcgaagct tttactggta
    14641 acggaaaagt tgaggcaatc aaaacaagtg aaaaagaaat cccagcagat ctagtcattc
    14701 aagctgccgg tgtgaaggca aatacagaat ggttaaaagg gatcgtcgac ttagatgaac
    14761 gtggctggat ccaaacaaat gaatacttgc aaacgaatct tccagatgtg tatgcagtag
    14821 gagatgctac acttgcttat tctatcccag cacgcaaaaa aatgccgatc gctttagcga
    14881 ctgttgcacg tagagaagca cgttatgtag taaaacatct ttttgaagaa gtaccaaaca
    14941 aaccgttcgg cggagtcgta ggttcctctg cattaagtgt ctttgactat cattttgcag
    15001 caagcggatt gaatagcttt acagcagcaa aagcaggagt agatgtacga gtgtcttatt
    15061 atgaagatac gttacgacca aaatatgtac cagaagaaca aggaaatcca aaagtgtcca
    15121 tccaattatt ctcggatcca atgacccatc aattgctagg aggagcagta ttatctacct
    15181 atgatgttac aggtcaagga aatgtattgg cactagcgat ccaaaacaaa ctgactttag
    15241 aagaactagc agaagctgac ttcttcttcc agccaggctt tgaccgacaa tggagcatgt
    15301 tgaatctagc cgctcaacaa gcgttaggag aagaaccatt tgtagaataa gaaaacgaaa
    15361 tatttgaaaa gaggttgtga aaaagctgcc ttgcatcaag taataagaac agccaagtaa
    15421 aaacagcttc ttatgttttt aacttggctg gacttattac cgcagatgca agcttttgaa
    15481 caccgtttat tttcaaaaaa gggactgtga cacgatttgt gtaccgaccc ccaaaagtta
    15541 gaccaaaaaa tctaactttt ggaggtcggt attttttatg gttaaatata cttttgaact
    15601 taaattaaag atcgttcatg attatttgga tggcaaaggc gattggaccg taaaaatcca
    15661 gatcaagaga ttgaagcgga aatgcttaaa attcgggaga agcacaagga ttatggctgt
    15721 ttacggatga ccaatgaact aagaaaccgc ggtttctcta tcaataaaaa gaaagttcag
    15781 cgcttgatca agaagctagg aataaaagta acggcataca cgcgtaaatc acgtcgctac
    15841 aattcttatc gaggaaaagt
//
LOCUS       DACKAC010000066        14602 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.067, whole genome shotgun sequence.
ACCESSION   DACKAC010000066 DACKAC010000000
VERSION     DACKAC010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.067"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            131..505
                     /locus_tag="I0B46_002169"
     CDS             131..505
                     /locus_tag="I0B46_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAR2084440.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="I0B46_002170"
     CDS             complement(619..1248)
                     /locus_tag="I0B46_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAR2084441.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="I0B46_002171"
     tRNA            complement(1382..1454)
                     /locus_tag="I0B46_002171"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="I0B46_002172"
     tRNA            1668..1741
                     /locus_tag="I0B46_002172"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            1696..1845
                     /locus_tag="I0B46_002173"
     CDS             1696..1845
                     /locus_tag="I0B46_002173"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084442.1"
                     /translation="MTVNHDVVGSSPTAGVNKAYAKKKKSNHLGFFFFYYYDESSFLF
                     LSRML"
     gene            2338..3666
                     /locus_tag="I0B46_002174"
     CDS             2338..3666
                     /locus_tag="I0B46_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAR2084443.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..4701
                     /locus_tag="I0B46_002175"
     CDS             3868..4701
                     /locus_tag="I0B46_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAR2084444.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4717..5454
                     /locus_tag="I0B46_002176"
     CDS             4717..5454
                     /locus_tag="I0B46_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAR2084445.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5609..6577)
                     /locus_tag="I0B46_002177"
     CDS             complement(5609..6577)
                     /locus_tag="I0B46_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAR2084446.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6809..7642
                     /locus_tag="I0B46_002178"
     CDS             6809..7642
                     /locus_tag="I0B46_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAR2084447.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7676..8515
                     /locus_tag="I0B46_002179"
     CDS             7676..8515
                     /locus_tag="I0B46_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAR2084448.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8541..9101
                     /locus_tag="I0B46_002180"
     CDS             8541..9101
                     /locus_tag="I0B46_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAR2084449.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9300..11021
                     /locus_tag="I0B46_002181"
     CDS             9300..11021
                     /locus_tag="I0B46_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAR2084450.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11186..12328
                     /locus_tag="I0B46_002182"
     CDS             11186..12328
                     /locus_tag="I0B46_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAR2084451.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12344..13555
                     /gene="thiI"
                     /locus_tag="I0B46_002183"
     CDS             12344..13555
                     /gene="thiI"
                     /locus_tag="I0B46_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAR2084452.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            14128..>14602
                     /locus_tag="I0B46_002184"
     CDS             14128..>14602
                     /locus_tag="I0B46_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAR2084453.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSK"
BASE COUNT         4989 a         2431 c         2959 g         4223 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     3961 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     4021 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     4081 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     4141 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     4201 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     4261 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     4321 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     4381 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     4441 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     4501 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     4561 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     4621 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     4681 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     4741 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     4801 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     4861 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     4921 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     4981 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     5041 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     5101 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     5161 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     5221 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     5281 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     5341 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     5401 cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaataa ataaaaaaga
     5461 ggttgtgaca gaagggggca acttcaagaa ataagaagaa atttacgaaa attgtttctc
     5521 aaatttttgt aaattttcga cttagttttc gaagaagcta tttttgtcac agcctctctt
     5581 tttaagttta gctattagat aatgatgttt agttttggat aaacgaaatc aattcttcac
     5641 ccgacaaacc atagtttaca gcaatcaata gttttaaacg tgctttttga ctattaatac
     5701 tgttacagaa aataatcccc attttttcta gttcttttcc cccgccttca taatcataaa
     5761 caggctctgc gattccattg aaacaacgag aaacaagtac tacagggatt ttttggacaa
     5821 gaagcttttc caaagcagct aaggcttgtg gagggagatt ccccgcacct aaagcttcga
     5881 ttaccaaacc gtctactttc gtatgggcga tggcttccaa taagtcccct tgcatcccag
     5941 catatgcttt aacgatcggg atagtcccat ctacagcgga aatgtccagt cgctttgttt
     6001 ctaaaagttc ctgcaagaat aaaattcgat tcttagttac taagccgatg ggacccaatg
     6061 taggtgttcg gaaagtagca acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat
     6121 ggatctcatc gttcatgacc accaaaaccc ccttatctag cgcttcttca caacttgcga
     6181 cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc aagttcatta cttgaacgca
     6241 ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag
     6301 tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc ggatatacct gatgcataag
     6361 cggacaagat acgttctttt aataccaaca tgtgagaagg catgatgtgt ggtgagggaa
     6421 gattgaagat atcttcaact aataattcta catgcttggg gattgtgatt tctgcatcta
     6481 ataatgggtt tgccacatca ggactaacac cgcccgtgag catgttttct ttcatggcga
     6541 tcgttccgcc ggtgtgtagt acaagtattt ttttcatttt acagacttcc ttacgaataa
     6601 ttttgcatag ccgttaaatc ttattgtata ctatataaaa gagttttgct atcagtgaag
     6661 aataacgaat agcctaactt atcataaaaa agctgacctt attaaaatgg aaaagactca
     6721 accttgacct cctaatacaa tggagtatct ggttgagttt cttttacaag aaagcagcca
     6781 aggaaatcaa tcacgaaggt ggccggaaat gatcagaatc ggattaacat cgtttaaaga
     6841 acatgacaaa ttaactggta aaaagaataa cactctttat gaatatgcag ggtttctacc
     6901 cttagtggaa ttagatactg cctattatgg aattccccga aagtcctcag ttgagaattg
     6961 gataagtgaa gtacctgaat catttcgctt tgtagtcaaa gtctatcaag gaatcagcgg
     7021 tcaaggagac tggaagcctt attatcagac cgaagaagag atgattgcgc attttttaga
     7081 tgttatgtcg ccaatggtag atgctggaaa gatgttttgc tttttagtcc aattcccctc
     7141 atcattcaaa tgtacgaaag aaaatgtcca gtacttgcgc aaacttagaa agtggtttga
     7201 gggttatcct gtttctatag aattgcggga ttattcttgg tacagcgatg aatttcttga
     7261 acgtacaaga aactttatgt ctagagccgc tttttcttta gccatcatcg atgagccaca
     7321 gttgctgaat acaacgattc cttttgatcc atttgtcacc aatccgaact ttgctctttt
     7381 tcgattccat ggtaggaatg cgcaaggatg gcaagcaaat gataaagagt ggcgtaaaaa
     7441 aagaacgtta tatcgttatt cagaaaaaga actgcaagaa ctagcacatg aagtcaaagt
     7501 agttgattct aagacaaaag aggtaggtgt gatttttaat aataattctg gtggtgatgc
     7561 ggctgaaaat gcattagcac tgcaaaaaat attaaatatc aagccagatg gactcaatcc
     7621 aacccaaatg gatctgtttt aagggcttat tttattcaag aaaggggcga agtctatgat
     7681 caaagctatt ttttttgata ttgacggaac attggtcacc aatcgttcaa aagcgttgga
     7741 atcaacaaaa caagcaattg aatatgctcg gaaaaacggc attttatgcg gtgtagcaac
     7801 ggggcgcagc ccagtaaaga tcaaagaaat cattgatgaa ttggaattag atatgtatgt
     7861 cgtgtataat ggtcaactgg tttttactgc agatcgcacg attatcgacc acccttttga
     7921 acaaaaagta ttggaacata ttgttgaatt tgcagatgag aaccatcgac aaatcgtttt
     7981 tggtgcgaga aatcggttag atggatcaac gacgatgcta ttaggacaat cgatttttat
     8041 caagcgacta gtcagctttt taccgcgtaa attcccggta cgtctgatga aaaaaatcct
     8101 ccaggttttt agccctcacc gacaaaagga tcgttatgaa acgttagaga tattaaaaga
     8161 accaatttat caatgtatat tgctcagtcc ggagtcagaa cagaaaaagt tagaacatcg
     8221 ctttcccgag tgtacgtttc agcgttcaaa tacgtatact gtagatatca tcccaaaagg
     8281 tggttcaaaa ttactaggca tccaagcttt cgcaaatgct gtcgaaattg aaatggaaga
     8341 aattatggca ttcggcgatc attacaatga tatcgagatg ctgaaaggtg taggaattgg
     8401 agtggccatg gggaatgctc agatcgaagt aaaacatgct gcagactttg tgactcaatc
     8461 aaatgaagaa gatgggatat acgaaggact gaaacacttc ggtctgattt attagtcaat
     8521 gaaagaagga aaaaatcagt atggaagcag agacaccttt tcaaaaagta gaaacattcc
     8581 acaaaacttt tgatccaaga agaccagaga tacccacggc ttttacagaa aatgaagctc
     8641 atacacgtgc tgcgttcaaa atagaagaac ttgttgaatt tgttgcagct acttcaccta
     8701 ataaagaaca actgcatgaa gctgttatgc tgctgcacga agcattagat aaagcagaag
     8761 aaaaaatact tggtaaaaca cagtggggaa gtgatccgct ggttgaacaa gtagatgcac
     8821 tgacagatat cctttacttt acttatggtt ctttttcatt actaggaata gtgcctgatc
     8881 gaatattcga aatcgtccat caggcaaata tggggaaatt atttccagat ggaaaacctc
     8941 attatgaccc tgtaacaaat aaagtgttga aacctgaaaa ctggcagatg gaatatgctc
     9001 cagaacctaa aatcgcggca gaaatccaac gtcagattca agaagcgatt gctaagaaga
     9061 aatccgctga tataggcatg tgcacaaatg aaaaagagtg atcagagttt cactattttt
     9121 gttatcttta tacgatggaa aaacaaaaga ttttcgttta agttagtgaa tgcatgaaaa
     9181 tagtatgcta taatctagaa tagatgtctt ttgcacgaga gacgtattat ttggatttat
     9241 gccaagcaga acgccatctg ttgcataaaa gatgcatact gaaaacggag gaaccataaa
     9301 tgggaaataa tgttatcatc ggaatcgtca ttgcaatcat tatagtcgcc attgtcgtct
     9361 ttttggttag ttacttgatg aagaaaaaaa accaagatcg attggatgcc ttagaaaaac
     9421 gcaaggaaga attatttgac ttgccagtga ttgaagaagt cgatgatgtt aaaaaaatgc
     9481 atctggttgg gcaaagccaa aatacctttc gtgaatggag tcaaaaatgg aatgacatat
     9541 ctacttcctc gttcgctgaa ctagaaagtc aaattttcga agtggaaaac ctcaatgaaa
     9601 gttttcggtt cttaaaagga aaaaaagcag ttgttgaagc agaagctaca atggaccaaa
     9661 tggagaaaca agtgacggaa atccgtgaag gtctaaagga attgcgggaa acggaagaac
     9721 gcaactcact agctgttcag caggcacttg acgtctatga agaactgaaa gaaaaagtca
     9781 gagaagacga gcatgaatac ggaccagctc tttttgaatt gcaaaaacag atcaaagtga
     9841 tcgaaaaaga atttacgcag tttgtgactt taaacacttc tggagaccct gttgaagccc
     9901 gcgaggtttt ggataaagca gaaaaacata cgtatgaagt ggaagctctg atgaaaaaaa
     9961 ttccaccgct gtatgaggag ctccatacca ctttccctga acaacttgaa gaaatcagtg
    10021 atacttacga gaaattgatg gaagaacaat atgtattccc agaagaagat cttgctgaag
    10081 atatcgcaaa agtaagtcga agaattgaaa attcattagc caatcttgaa aaaacagaag
    10141 ttgaaacggt cgaatttgaa aaccgtgaaa cagctgattt gattgattcg ttatatgata
    10201 tcttagaacg ggaaatggaa gctcagagat atgtaaaaac taaccaatca acgattgcag
    10261 agtatatcaa acatacaaca aagaataacc gtcaattatt gattgagctg gatcacacat
    10321 ctcaaagcta tacattgaac cacaatgaaa ttggccgtgt acgaggtttc caaaccgaag
    10381 tggaagaaat ggaacgtcaa aatgaacaga tgattccaca gatccgtcaa cacgaaattc
    10441 cttattcaga agtgcgcacg ttctataaaa cagtcttcaa agtcttagaa gatatcgaaa
    10501 agcaacaagt agagatcgac gactcattac atgaattgag aaaaggcgaa aaagaagcgc
    10561 aagaaaagat cgatacattc gaattcaaat tgcgcagttt gaaacgtttt gtagaaaaac
    10621 aacgtttgcc tggcttgccg aatgattacc tagaattctt ttttgttgct actgaccgta
    10681 ttgaagaact aagtgttgtc ttgaataaaa ttcgagtaaa catggaagag gtcaatcgtt
    10741 tagttgctct atgtgaagaa gatctagaat tattagataa gaagacacat gatttagtag
    10801 atgcagcagc attaacggag caaatgctcc aatatgcaaa tcgttatcgt catacacacg
    10861 aagaagtgag agaagcaatt gataaaagct actatttatt caataaagaa tatcactatc
    10921 aagaagcatt agacgaaatc ggtacagcat tagagcgagt agaacctggt gctttcaaac
    10981 gcatcgaaga tttttatttt aatcatccag acttagtata aagcaaaaaa gttagtaaac
    11041 cagcaagagc aagacggatt attgtcttgt tcttgtcttt ttttgtatat ttgaaaattg
    11101 aaacaagtga aaatttcacc tgctcttgaa agcaccttgg acaaccgtta taatagaaaa
    11161 gaatttcaaa aaagaggggt ttcttatgat atattttgat aacagtgcaa caaccccaat
    11221 ctttccgcaa agcttagatg catatgtaaa aaccagtcag cggattattg ggaatccatc
    11281 aagcttgcat gatttaggca atcaagctaa ccggttgttg cagcaagcaa gaaagcagat
    11341 cgctgatttg atccatgtag aaccagaaga aatctatttt acttcaggcg gaacagaagg
    11401 agataactgg gtattaaaag gaacgatgat cgaaaaaaga gaatatggga atcatatgat
    11461 catttcagga gtcgaacatc cggccgtcag cgagacagca gaacaattaa aagaattagg
    11521 aatcgaagta agtatcgccc cagtggataa acgcggattt gtacgtgtcg acgaattgaa
    11581 agaattaata agaaaagaga cagtattagt ctctgttatg gctgtgaata atgaagtagg
    11641 atcgattcag cccatccagg aaatcagtga tcttttggat gattttccta aaattcattt
    11701 ccatgtagat gctgttcagg cgattggaaa agtaccattc tcgcaatggt tgacagaaag
    11761 agttgatttt gcaacatttt cagctcataa attccatggc ccacggggaa ctggtttcat
    11821 ttattggaaa aagggtcgtc gtttagcacc attactgaca ggtggagggc aagaaagaaa
    11881 tcagcgaagt gggacagaaa atgttccagg tattgtggct atggttaaag ctttacgttt
    11941 atcattagag ataaaggaac aaaaacctga gcagacacaa cgcttaaaag tagctctaat
    12001 caatgcgttg aagacgtatg agaaagtgac gattttttca gaaggaactg actatgcacc
    12061 gcatatttta tgttttgctc taaaaggaat tcgcggcgaa gttctcgtcc atgccttaga
    12121 agaaaaacaa atatttgttt ctactactag tgcctgctcc tcacgtaaac atatggcaag
    12181 cagtacattg cacgcaatgc atgtaccaaa tgatctggct acatctgctg ttcgaatcag
    12241 cttagatgaa aacaattcgc ttgcagaagt cgagcaattt atgattattt tcaagcagct
    12301 ttataagaaa ttttctgcaa tcaattaaaa gaggagtgtt cttgtgaaat acagtgaaat
    12361 tatggtccgt tatggggaac tttcgacaaa aggaaagaat cgaaaaagct ttatcatgca
    12421 actagcacaa aatgtcagac aagctttgaa agactttcca gaaatcaaaa tccatgctga
    12481 tcgggaccgg atgcatcttt tattgaatgg cgcagacggg cagctagtta ttccaaaact
    12541 agcaaaaata tttgggatcc aaaatttctc tccaagtatt cgtgtagaaa aagatgtcga
    12601 agttttgaaa caagctgttc aagacatcat gaaagaaatt tatactggga aagaaacatt
    12661 taaaattatc gctaaaagaa gcgatcacca atttgaattg acaagtaatg aattgaatca
    12721 aacattaggt aatgctgttt ttgatatttt cccgcatatc caagttcaaa tgcgtcaacc
    12781 agatattcca ttacgtgtag agattcggcg tgatggcgct tatctatcgt atgaaacgat
    12841 caagggagct ggaggattgc ccgtaggaac aagcggaaaa ggaatgctta tgctttctgg
    12901 ggggattgat tctcctgtag cagggtactt tgccatgaag cggggagtgg aaattgaagc
    12961 tgttcatttt gctagtccgc cgtatacaag tgaacaagca ctgcaaaaag caaaagatct
    13021 aacagccaaa ctagcacctt atgtgggaac gatccatttt atcgaagtgc catttaccga
    13081 gattcaagaa gaaatcaaaa agtcagtgcc acaaggatat tggatgacaa ttactcgtcg
    13141 aatgatgttg cgtttgaccg atgaaattcg ccgtattcgt catggtttag tcattttgaa
    13201 tggtgaatca ttaggtcaag tggcttcaca aactttgcaa agtatggtgg ctatcaatga
    13261 agtaacgaat acgccaatca ttcggcctgt agcgacaatg gataaacttg aaatcattga
    13321 agtagcagaa cagatcgata catttaatct ttctatccag ccttttgaag attgctgtac
    13381 gatttttgct cctccacagc caaaaacacg ccctcaactg gaaaaggtcc atcaatatga
    13441 agagcgttta gcaatagatg aaatgatcca acgagcacta gctggcttga agatggaaga
    13501 gatacaagca gaatcgtcta atgaaaaaac tgaagaattt tcagaattgc tataagaggt
    13561 tatgaaaata gtgttttgac cagagtatta gagaaacaaa acgaaaaaat agctccatgt
    13621 attttaccgt ttttgaacta atgtcgaaga ttagctgatt gaataccatt tatcaagaag
    13681 ttatggaaaa gccggttctt ttttactaac tgaaaaataa aattttattt agagatcaag
    13741 ctacagttga taaatgatat aaaaaagaga atttcccatg aaatagcgag gttgtgacag
    13801 aagtgggcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttttca aatttttgtg
    13861 aaatttcggc ttatttttcg aagaagttac ttctggaaca ccgtgtatca gaggttgcga
    13921 aaaagctgcc ctgcatcaag taataagaac agccaaacaa aaatagttcc tcatatttta
    13981 cgtttggttg agcttattac cgcagatgca agcttttgaa caccgtttta tttgtctcac
    14041 acccttttcc ttcctacttg taaccttgct cccgtgttag aaacatgtta cactaaatat
    14101 gtgaaattat taacaaggag cgtcgttatg cgagaaaaaa cagatacact gccaaaagca
    14161 actgcgaaaa gattaccttt atatttacgc tacttgaaga tgctggatga ctcaggtata
    14221 agtagaatca aatcaaatga gttcagcgaa atcacccaaa ttccttctgc aacgatccgc
    14281 agagacttct cgcaattagg cgagctaggc agaagcggat atggctatga tgtccctttt
    14341 cttatcgatg tattcaataa tatccttaat actaaagaag aaaaacgaat cgcattagta
    14401 ggatatggaa atcttggcaa agcattgaaa cacaacaatt ttagaagaaa tgaaaatcta
    14461 aatatcgtgt gcgtctttga taatgatcca gcactgatca atcgagtaat tgatggagaa
    14521 atgatctatc cgattgatcg tttcgcggag atagccaaag caaaaaatgt gacagtagca
    14581 atctctacgg taccaagtaa at
//
LOCUS       DACKAC010000067        14552 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.068, whole genome shotgun sequence.
ACCESSION   DACKAC010000067 DACKAC010000000
VERSION     DACKAC010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14552
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.068"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..153
                     /locus_tag="I0B46_002185"
     CDS             <1..153
                     /locus_tag="I0B46_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAR2084454.1"
                     /translation="NKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVSTEIKV
                     LMGITA"
     gene            318..699
                     /locus_tag="I0B46_002186"
                     /pseudo
     CDS             318..699
                     /locus_tag="I0B46_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303242.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            830..1780
                     /locus_tag="I0B46_002187"
     CDS             830..1780
                     /locus_tag="I0B46_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002821555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAR2084455.1"
                     /translation="MLKTYSLTKDTLLVSSESNIKNSSVVYVYGDEREYIESSEHKYD
                     FKIGNILTFDDRVAYDTMIDKNDEKSLLVSFLFPEVHEGGHLDTLTTSVVFLVFKNKL
                     IIFTKQKLKFIPELLSNMVLRDVNHFVEEVLLKIMQKDFYMMLNDLRGVKEKIDDLDS
                     EISTSGSLKPTFGDLLTLQKYMIALSTTYKANHKALDFIKKNFTTIDDIDFSTKKIID
                     DLYDTSDTMDRIILSYSQYLDKLENMINNMISYQLNMIMKTLTEISIVLTIPAIIFGF
                     WGINVHVPFEKSSYGVLAVLIISAILSLICWLLMRRKTYL"
     gene            2298..2753
                     /locus_tag="I0B46_002188"
     CDS             2298..2753
                     /locus_tag="I0B46_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAR2084456.1"
                     /translation="MVQQIVLPIKDSSVLKMVQDTLLDSFRAGRRNYTVFQVGKATLL
                     RVSDVMTLKKSDVYNPDGSVKNATFIHDKKTGKANTLYLKPVQQDLLQYHDWLAQENI
                     NSEWLFPSTAHYDLHITEKQFHKVMAHVGDLLGINLEDKEKKLRVRKPF"
     gene            3064..3420
                     /locus_tag="I0B46_002189"
                     /pseudo
     CDS             3064..3420
                     /locus_tag="I0B46_002189"
                     /inference="COORDINATES: protein motif:HMM:NF013878.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3433..3843
                     /locus_tag="I0B46_002190"
                     /pseudo
     CDS             3433..3843
                     /locus_tag="I0B46_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812298.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
     gene            3958..4122
                     /locus_tag="I0B46_002191"
     CDS             3958..4122
                     /locus_tag="I0B46_002191"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084457.1"
                     /translation="MSYPRTDTPFITENEFAYLKANFGKYSGFLGLDLEMLLSLIMIR
                     SNNSTQILTN"
     gene            complement(4273..5252)
                     /locus_tag="I0B46_002192"
                     /pseudo
     CDS             complement(4273..5252)
                     /locus_tag="I0B46_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332307.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(5257..6024)
                     /locus_tag="I0B46_002193"
     CDS             complement(5257..6024)
                     /locus_tag="I0B46_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAR2084458.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
     gene            6514..6939
                     /gene="lacA"
                     /locus_tag="I0B46_002194"
     CDS             6514..6939
                     /gene="lacA"
                     /locus_tag="I0B46_002194"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HAR2084459.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            6956..7471
                     /gene="lacB"
                     /locus_tag="I0B46_002195"
     CDS             6956..7471
                     /gene="lacB"
                     /locus_tag="I0B46_002195"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HAR2084460.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            7482..8414
                     /gene="lacC"
                     /locus_tag="I0B46_002196"
     CDS             7482..8414
                     /gene="lacC"
                     /locus_tag="I0B46_002196"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HAR2084461.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTISHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            8417..9397
                     /gene="lacD"
                     /locus_tag="I0B46_002197"
     CDS             8417..9397
                     /gene="lacD"
                     /locus_tag="I0B46_002197"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAR2084462.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            9425..9742
                     /locus_tag="I0B46_002198"
     CDS             9425..9742
                     /locus_tag="I0B46_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAR2084463.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            9742..11448
                     /locus_tag="I0B46_002199"
     CDS             9742..11448
                     /locus_tag="I0B46_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAR2084464.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTLLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            11590..12996
                     /gene="lacG"
                     /locus_tag="I0B46_002200"
     CDS             11590..12996
                     /gene="lacG"
                     /locus_tag="I0B46_002200"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAR2084465.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRVKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            13019..13291
                     /locus_tag="I0B46_002201"
     CDS             13019..13291
                     /locus_tag="I0B46_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HAR2084466.1"
                     /translation="MLSDTLEKVQHLFRPGFEKVYIVSFEDCPMIPELEATPLLKCGK
                     WYVSTGKEWICHSDLELSAFEWEFLQSLDVEIRETIHFEVNYLPFQ"
     gene            13312..14211
                     /locus_tag="I0B46_002202"
     CDS             13312..14211
                     /locus_tag="I0B46_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAR2084467.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDKDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSPLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
BASE COUNT         4755 a         2401 c         2811 g         4585 t
ORIGIN      
        1 aataaatttt atcaaagtct ccgtacagcc tcttccacga ttaagggcat ggagaccatt
       61 cgaggaatat ataaaaagaa ccgaagaaat ggaacgctct tcggcttttc ggtgtctact
      121 gaaatcaagg tattaatggg aataacagcc taagatattt ggagttcaga gagggtgcgt
      181 ttgattttca aacttcgcaa cagaaccgat gacactatcg ttcttcatca acgtaagtta
      241 gatttgttga aagagcagaa aaaaggattt ctacaaaaaa tgtttgtttg atattttaaa
      301 tagaaaagag agataaagtg aagtttttaa attttttgaa ctataaatat ttaaactggg
      361 tattatatgg tattacatta ttttttatta tttattactt ttttaccgat gatgatattt
      421 ttttaatttt agcaatactt tttcttgttt catctaattt tagtgataac ttaattgaag
      481 ttcttacaga accagaaatt cggtctatga cggatgaaga acaacagatt attaaagcta
      541 tgatttcgga aaataagaaa gacgtagtag ttatcaaaaa gattagagag tatacaaatt
      601 taggtgtttt acaagccaaa aaagtatatg aaaaaaaaaa aaagggtcta taattacaaa
      661 ttagataata cattagaagc tggagacaag attgtctaga taatgtgtga gataaaattt
      721 ttggcaggtt atcatacata agacaaaatg tttttaaaac tgataacgta tttattccac
      781 aacaattacg catttatttc tgagaaaaat ataaagcagg aggattacaa tgctgaaaac
      841 ttattcctta acaaaagata cattattagt ttcgtcagaa tcaaatatca aaaattcttc
      901 tgttgtttat gtttatggtg acgaacgaga atatatcgaa tcttctgaac ataaatatga
      961 ctttaaaata ggcaatatac taacgtttga tgatcgagtt gcatatgaca cgatgataga
     1021 taagaacgat gaaaaaagtt tgctcgtaag ttttttgttt cccgaggtac atgaaggtgg
     1081 gcatctagat accctgacga catctgttgt tttcttggta tttaaaaata agttgataat
     1141 ttttacaaaa caaaaattaa aattcattcc cgaactctta tccaatatgg ttttacgtga
     1201 tgtcaatcat ttcgtggagg aagtactttt aaagatcatg caaaaagatt tctatatgat
     1261 gcttaacgac cttcgtggag tcaaggaaaa aattgatgat ttagactcag aaatatcaac
     1321 gtccggttca ctcaaaccaa cgtttggtga tttattgact cttcaaaaat acatgatagc
     1381 tttgtcaaca acttataaag ctaaccataa agcgcttgat ttcattaaaa aaaatttcac
     1441 aactattgac gatattgatt ttagtactaa aaaaataatt gatgatttat acgatacaag
     1501 tgatacaatg gatagaatca ttttaagtta cagccaatat ttggacaaat tggaaaatat
     1561 gataaacaat atgatttcgt atcagctaaa catgattatg aaaaccctaa ccgagatatc
     1621 aattgttttg actattccag caattatttt tggcttttgg ggaatcaatg tccatgtccc
     1681 gtttgaaaag tcttcttatg gtgttttagc cgtattgatt atctctgcta tactaagtct
     1741 aatttgctgg cttttgatgc gacgcaaaac atatttgtaa agtatttgtg ataccctaat
     1801 atgatttaaa gcagaatttc tttgttgact ttttaaaagg ctgattcctt tcaataattt
     1861 aatagcttca gcggctgctc agaagtcaat aaattggact atttttattc agttacttag
     1921 gcaacatttg agatacgatt gcgattataa aaattgatat accaaccaat ggctgcttgg
     1981 aagactgaca tattagcctc gatatcatcg aataactaaa tacagaaaaa tacaataaat
     2041 attatcaaac tagggactat actagaaaac atcagtacat tccttaactc agggtctata
     2101 attagaaaat cgcccctttg aaaactgatt aaaacagccc ccattatcta gtgactagat
     2161 aatgggggct gtttttttgt taagtgatat aataaaccat aaaagaagtt tagaaatacg
     2221 gtcctggtca tcaataaata aacaatttaa ttaattattt atttaattat ttaatttcag
     2281 aaaggtatac atgaatcatg gtgcaacaaa ttgtcctacc catcaaagac tccagcgtct
     2341 taaagatggt acaagataca cttctcgata gtttccgtgc tggtcgccga aattacaccg
     2401 tttttcaagt tggtaaggcc acgctactac gtgtgagtga tgtcatgaca ttaaaaaaat
     2461 cagatgtcta taatccagac ggctctgtca aaaatgcaac ctttattcat gataaaaaga
     2521 ccggtaaagc aaacacgtta tatttaaagc ctgttcagca agacttgttg caatatcatg
     2581 attggctggc ccaagaaaat atcaattctg agtggttatt tccctcgaca gcccattatg
     2641 atcttcatat cactgagaag cagtttcata aggtgatggc gcatgttggt gatctcttag
     2701 gaatcaactt ggaagacaaa gagaaaaaat tgcgagtcag aaaaccgttt tgagatatga
     2761 acagcatttg ccttcacgcc ttagcgattt taaataatag ccttgaaagt gattatggaa
     2821 aagccctcta cgctcaatgc aaaattgacg tagaaactat ttttgatcga ttgaaaggtg
     2881 tatttggtat gtgaagaacc catgtcagag gcaagaacgt tgcacctact gacattggaa
     2941 tgatgcttat gagcatgaat ctaaccaaat tatcccttga ggctagaaga aaagcggaag
     3001 cttttcaaca caaagcagcc aaaaataaaa aacgcgacga agcaatcaca tttatgatta
     3061 ttcatcgcgt tttttatatt tagagctagt tatttgctag acccactatt tacagatgaa
     3121 acgtttatca cctttggttt tgggcattta gtggaattag cagaaccagg tcattatgac
     3181 gaaaagtggc aaaattggaa acttgaatct ttgccgattt ttcctgatcg atacgatttt
     3241 gaagttgcaa aagataaggg aaagcagttt aaaattgttg cagaacttct caaaaaggca
     3301 aatacaatta ttgttgcaac agatagcgac agagaaggtg aaaatatcgc ctggtcgatt
     3361 atccataatg caaatgcctt ttcaaaagat aaaacattta aaagactatg gatcaatagc
     3421 ttagaaaaag atgtgatccg aagcggtttt caaaatttgc aaccaggaat gaattactat
     3481 cccttttatc aagaagcgca aacacgccaa attgccgatt ggttgatcgg catgaacgca
     3541 agccctttgt atacgttaaa tttacaacag aagggcgtac aaggtacatt ttcactagga
     3601 cgtgttcaaa cgcccacctt ataccttatt tttcagcgcc aggaagccat agagaatttt
     3661 aaaaaagaac cttttttcga ggtggaagct aagtaaaaca agggtcattt aagggcgttc
     3721 taagccccac acagcgtttt aaaacccaag aggagctttt tagcttttgt ttcttctaaa
     3781 caagctaaaa taggcaatca agaggggata attgctgatg ttcaaaccaa agagaagaaa
     3841 acgaatagtc cgagttcgtt ttctttaagt agtttgcaat caaaagtcaa tcagctttat
     3901 aaagcgacag cgagccaaac tttaaaagct atgcaaggac tgtatgaagc aaaattattg
     3961 agttatccaa gaacagatac accatttatt acagagaacg aatttgctta tttaaaagcg
     4021 aattttggca aatatagcgg ttttttagga cttgatcttg aaatgttgtt atcattgata
     4081 atgatacgtt caaacaacag cacccaaatt ttgacgaatt agtgaaactc tatgaaaaag
     4141 acgtagtaaa acatgcaaca tcctattcta atcagctggt aaagcttaat taggttatat
     4201 aagcttgaaa ataaagggtt tgtccaatat ggacaagcct ttttcttatg ttttgccaca
     4261 gatttgccac acttattgtg taagagtatc aaggataggt ataatttgat actttgtttt
     4321 tttagtgatg tgcaaataaa tttctgttac acgactacct ctagaatgcc ctacacggtc
     4381 ttgaattgct tctaggggta ttccttactc tgctaagaaa ctaatatgcg tatgacggaa
     4441 tatatgggtc gtaatatgtt tattaaaact aagtttatta tcaatatcaa ttcctgctcg
     4501 ttttggatta ccttttcgat ttaattttcc attttcaggt gagttgagga aattgttaag
     4561 gacactataa tgaagaggtg ttccattata gtcgggttta tgacgacttt tggaagtaaa
     4621 aatccatcca ttatctttat aatcaggatt gaatctcata tgttggtgat ttaactcaat
     4681 ttcctcttta agaatttcta tagccctttt agataattgg acatttcggt aactttgtaa
     4741 gttttttaca ttaccttcat agctattttc tgcagctcct ccgtgagtgg actgactaat
     4801 gatgatattt tgcttttcaa aatattgata ggcaccaata ttttggagtt tcgactctgt
     4861 aagtcaacag ggccattagt tagaatacct attgggatgc ggtttttatg tagagccagc
     4921 agtagttgat gtatctcagg aatcatcgta atctgatcga gttcatattc atagtcagct
     4981 tggaagtcta gagcttctgt cctagaaata ctatcgatgc caaattcttc catagtcttc
     5041 tgacagcgaa aaattctaag ctgctcaata ctgatgagtt ttttcgtgta tttaggaaaa
     5101 gcaacatcag accaatatct gaaacgtttg taaatgttat ctatttgttg gatatcaatc
     5161 gttggaaaac atttctcaac gctacgttta aaggggagtt gatggtcata aagagtgtca
     5221 tcaaggtcaa aaacaatatt tttataagtc ataattctag tccgctttta aaatagtagt
     5281 gtactggcta aattcctcta agtgttgagg gctaatctgg ttatctgtaa tgatggtatc
     5341 gagttgatct agattataga agttaaagaa atcgtatcga tcgaatttag tactgtctac
     5401 taataagaat ttttctacag cattgtttag ggcaagttgt tgaatcacac cttccttgtc
     5461 actatatgta gcaatagaat tatgggtaac agcatttgca ctaacaaaag ctttggcaaa
     5521 tctaattgct tttaaatttg tcgaagccat tgaacctaca aaagctccag ttatttctct
     5581 atattcaccg ccaagaagca ataaatcaat ggtttcgcta tcatttagaa tcaagaacac
     5641 agggagacta tttgtaatga cacgaatttt ataaccttta cgacctttca attctaatgc
     5701 cagttgttca agtgtagttc caggtccaat aaaaatagta tcgccatctt tgattaaaga
     5761 gcaggctttt ttagcaatgt caattttttc ttttgtattt agacttttct tctcgatatg
     5821 tgtcttttca agtggctttt tagaggaaag caattgtgca ccaccatgag tacgtgttaa
     5881 aagtccatca gcttctagag catcaaggtc tctacgggct gtcatatcgg aaatatctag
     5941 ttcatctatt atttctttta tggttatggt cccatcaatc tttaacatat ctaaaatttt
     6001 ttctaatcgt cgttttttgt tcatatgaag acccttcttt cataaagtaa tttttttcca
     6061 aagataattc tcttttaatt gtatcataaa agacaatatt ttcaaggtaa aacaaacaat
     6121 ttcaaacaaa aacaaatgtt agatgatgaa ataagaacag aggattgacg tatattagct
     6181 taggtcagat tttgtataag acgaaaataa agtaggacct cttaatcagt aagttataga
     6241 aagtaaaaga cttttgtaat acttgaatag atatttcacg tccattttgt gatggattaa
     6301 atgaacaaaa atgaacaata atttaacggt gttatctatt ttttaaaaaa acaaataaaa
     6361 aaaacaaaaa ataaacaaaa aaagtgttgc tttttgtttg aatgcttgat attatataaa
     6421 caaagaaatg atgaaaacgt tttttcgaac attttgcaaa atgttttcta cttctacgta
     6481 gcatttcttt ctaaaattta ggaggtggtc aaaatggcta ttgttgttgg tgcagatctc
     6541 aaaggtacga gattaaaaga tgttgtgaaa aattttcttg tagaagaagg ttttgaggta
     6601 attgatgtga ctaaggacgg acaagatttt gttgatgtta ccttggcagt tgcctctgaa
     6661 gtaaataaag atgagcaaaa cttaggtatt gtaattgatg cttatggagc tggtccattc
     6721 atggtagcaa ctaaaattaa aggtatggtt gcagcagaag tttcagatga acgctcagct
     6781 tatatgacac gtggacataa caattcacga atgattacgg ttggcgctga aatcgtcggt
     6841 gatgagcttg ctaaaaacat cgctaaaggt ttcgtcaatg gtaaatatga tggcggacgt
     6901 catcaaatcc gagtagatat gcttaacaaa atgtgttaag aaaaggagtt aaggaatgag
     6961 aatcgcaatt ggatgtgacc acattgtcac agatgaaaaa atggcagtat cagaattctt
     7021 gaaatcaaaa ggacatgagg tccttgattt cgggacatat gaccacgcac gtactcacta
     7081 ccctatctat ggtaaaaaag tcggtgaagc agtagtaagt ggtcaagcag acttgggagt
     7141 atgtatctgt ggtacaggtg tcggcattaa caatgcagtc aataaagttc caggtgttcg
     7201 ctcagcgttg gttcgtgata tgacatcagc tatatatgca aaagaagagt taaatgcgaa
     7261 tgttatcggt ttcggtggaa agattactgg tggtcttctt atgaatgaca tcattgaagc
     7321 tttcattgaa gctgagtaca aaccaacaga agaaaataaa aaattgattg caaaaatcga
     7381 acacgttgaa acacataatg cacatcaagc agatgaagat ttctttacag aattccttga
     7441 aagatgggac cgtggtgact accacgatta aggtataaca aatgattctg acagtcacac
     7501 taaatccttc agtagacatc tcttatcctc tagaaacatt gaaaattgat acagtgaata
     7561 gggtgaaaga tgttagtaag acagctggtg gaaaaggctt aaatgttact agagtcttgt
     7621 atgaatctgg tgataaagtt actgctacag gtttcttagg tggtaaaatt ggtgaattta
     7681 ttgaaagcga attagaacaa tctcctgtta gtccagcttt ttataaaatc tcaggaaaca
     7741 ctagaaactg tattgctatc ttacacgagg gtaaccaaac agaaattttg gaacaaggac
     7801 caacaatttc tcatgaagaa gcagaaggct ttcttgacca ctatagcaat cttattaaac
     7861 aatcagaagt tgtgacaatt tcaggaagtt taccttcagg acttcccaac gattattatg
     7921 aaaaacttat ccaacttgct tcggatgaag gagtagcagt cgttttggat tgttcaggtg
     7981 cacctcttga aacagttcta aaatcgtcgg ctaaacctac tgccatcaaa cctaataatg
     8041 aggaactttc acagttgctt ggaaaagaag taacaaaaga tattgaagaa ctcaaagacg
     8101 ttcttaaaga gtcacttttt agtggtatcg aatggatagt ggtctcatta ggtcgaaacg
     8161 gtgcctttgc aaaacatggt gatgttttct ataaggtaga tattcctgat atcccagttg
     8221 ttaatccagt cggatcaggg gactcaacgg tagctggtat tgcatcagct ttaaatagta
     8281 aaaaaagtga cgctgactta ttaaaacatg cgatgacatt gggtatgtta aatgctcaag
     8341 aaacaatgac agggcatgtg aatatgacta attacgaaac actaaactca caaattggag
     8401 taaaagaggt ataaaaatgg tacttacaga acagaaacgc aaatctttgg aaaaaatttc
     8461 agataaaaac ggtgttatct cagctttggc atttgaccaa cgtggtgctt tgaaacgttt
     8521 gatggcacag tatcaagata cagaaccaac tgtagctcaa atggaagaac taaaggtttt
     8581 ggttgctgat gaacttacaa aatacgcttc ttcaatgttg cttgaccctg aatatggact
     8641 tccagcaaca aaagcattgg ataaagaagc aggtcttctt cttgcctatg aaaaaacagg
     8701 ttatgataca tcaagtacaa aacgtttgcc tgactgcttg gatgtttggt ctgcaaaacg
     8761 tatcaaagag caaggcgctg atgccgttaa gttcttgctt tactatgatg tagacagctc
     8821 agatgaactt aaccaacaaa aacaagctta tattgaacgt gttggttctg aatgtgtcgc
     8881 tgaagatatt cctttcttct tggaaatcct tgcttatgat gaagaaatct cagatgcagg
     8941 ttctgttgaa tatgcaaaag tgaaacctcg caaagtcatt gaagcgatga aagttttctc
     9001 tgatccacgc tttaacattg atgttcttaa agtagaagta ccagttaacg ttaaatatgt
     9061 cgaaggtttt gctgatggtg aagtagttta cagtaaagct gaagctgctg acttctttaa
     9121 agcacaagaa gaagcgacta accttccata catctaccta agtgcaggtg tatctgctaa
     9181 attgttccaa gaaacattgc aatttgctca cgactcaggt gccaagttta atggtgtgct
     9241 ttgtggacgt gctacttggg caggatctgt agaaccttac atcaaagaag gtgaaaaagc
     9301 agcacgtgaa tggttgcgta ctactggatt cgaaaatatt gatgaactta acaaagttct
     9361 tgttaaaaca gctagtccat ggactgataa agtatagtaa aaacataaaa cggaggatat
     9421 tgttatgaac agagaagaga tgactctctt agggtttgaa attgttgctt atgctggaga
     9481 tgctcgctct aaacttttag aagcgcttaa agcggctgaa aatggtgatt tcgctaaggc
     9541 agatagtctt gtagtagaag caggaagctg tattgcagag gctcacaatt ctcagacagc
     9601 tatgttggct cgagaagctt ctggggagga acttccatac agcgttacca tgatgcatgg
     9661 tcaggaccac ttaatgacta ctgtcttatt aaaagatgtg attcatcacc tcatcgaact
     9721 ttataaaaga ggagcaaaat aatgcataaa ctcattgaac ttattgagaa agggaaaccg
     9781 ttctttgaga aaatctctcg aaacatctat cttcgtgcta ttcgtgatgg atttatcgct
     9841 ggtatgccag tcatcctttt ctcatctatc tttatcctta ttgcctatgt cccaaatgct
     9901 tggggattcc actggtcaaa agacattgag accttcttga tgactcctta tagctattcg
     9961 atgggtatcc tagcattctt tgttggtggt actaccgcta aagctttgac ggactcaatg
    10021 aaccgtgacc tacctgcgac caatcagatt aacttcttat ctacgatgct agcgtctatg
    10081 gtagggttcc ttttgatggc agctgagcct gcaaaagaag gaggcttctt gacagccttc
    10141 acgggaacaa aaggtctttt gacagcattt attgcagcct ttgtgacagt taatgtttac
    10201 aaggtctgtg tgaaaaataa tgttactatt cgtatgcctg aagaggttcc accaaatatc
    10261 tctcaagtat ttaaagactt gattccattc actgtagcgg ttgttctcct ttatggattt
    10321 gaactcattg ttaagggaac tcttggtgta accgttgcag aatcaatcgg tacccttctt
    10381 gctcctctct tctcagctgc agatggttac cttggaatta cacttatctt tggtgcttat
    10441 gccttcttct ggtttgttgg tatccacggt ccttcaattg tcgaaccagc aatcgctgct
    10501 atcacttatg ccaatatcga tgccaacttg catcttatcc aagctggaca acatgcagac
    10561 aaagttatca cttctggtac tcaaatgttt attgttacca tgggtggaac aggagctaca
    10621 ttgattgttc cattcttgtt catgtggatt tgtaaatcag aacgtaaccg tgccatcgga
    10681 cgtgcctcag ttgttccaac attctttggg gttaatgagc caatcttatt tggtgcacca
    10741 atcgtattga atccgatttt ctttgtaccg ttcattttcg ctccgattgt caacgtttgg
    10801 atctttaaat tctttgttga cacattgaac atgaactcat tctctgccaa ccttccatgg
    10861 gttactcctg gtccgttggg aattgtactc ggtactaact tccaagttct atcatttatc
    10921 ttagccggac tcttggtagt tgttgatact atcatttatt acccatttgt taaggtatac
    10981 gatgaacaaa ttcttgaaga agaacgttct ggtaaaacaa acgatgctct taaagaaaaa
    11041 gtagctgcaa acttcaatac tgctaaagca gatgccgttc ttggaaaagc aggcgttgct
    11101 aaagaagatg tagcagcaaa caataatatc acaaaagaaa ctaatgtcct tgttctttgt
    11161 gcaggtggag gtacaagtgg cttgcttgcc aatgccttga acaaagcagc agcagaatac
    11221 aatgttccag tcaaagcggc agcgggtagt tacggtgctc accgtgaaat gttgccagag
    11281 tttgacttgg ttatcttggc tccacaggtc gcttcaaact tcgatgatat gaaggctgaa
    11341 acggataaat tgggcattaa acttgcaaaa actgaaggag ctcaatatat caaattgaca
    11401 cgtgatggac aaggtgctct tgcatttgtt caacaacagt ttgattaata aatttgtaaa
    11461 agttatttga gacgagatta ttctttcaat tccttataat attgttaacc atatcattgc
    11521 atttcatttc cttggtttcc ggaactcaga atatcgtctc aaatgctttt tttgaaagga
    11581 tttatagtta tgactaaaac acttcctaaa gattttattt ttggtggtgc tacagctgct
    11641 tatcaagcag aaggtgcgac tcacacggat ggtaaaggcc cagttgcatg ggataagtat
    11701 cttgaagaca attattggta cacagcagag cctgcaagtg atttttacca taaatatcct
    11761 gttgatttag aattagctga aaaatatggc gttaatggta ttcgtatttc aattgcctgg
    11821 tctcgtattt tcccaactgg ctatggcgaa gtaaatgaaa aaggtgttga attctaccat
    11881 aatctttttg ccgagtgcca caaacgtcat gttgagccat tcgtgacttt gcaccacttt
    11941 gacacaccag aagttcttca ctcagatgga gatttcttaa accgcgaaaa tatagaacac
    12001 tttgtagatt atgccgcttt ctgttttgaa gagttcccag aagtaaacta ctggacaact
    12061 ttcaatgaaa ttggcccaat tggagatggt caatacttgg ttggtaaatt ccctccaggc
    12121 attcaatatg atcttgccaa agtcttccaa tcacaccaca atatgatggt ttctcatgca
    12181 cgtgctgtga aattgtataa agataaaggt tataaaggcg aaattggtgt agtacatgcg
    12241 ttgccaacca aataccctta tgatccagaa aatccagcag atgttcgtgc cgctgagtta
    12301 gaagatatca tccataacaa atttatcttg gatgcaacct accttggaca ctattcagat
    12361 aaaacgatgg aaggggtcaa ccacatccta gctgaaaatg gtggagaact tgatcttcgt
    12421 gatgaagact tccaagcact tgaagcagct aaagatttga acgatttcct tggtatcaac
    12481 tactacatga gtgattggat gcaagccttt gatggtgaga ctgaaatcat tcacaatggt
    12541 aagggtgaaa aaggaagttc taagtaccag attaaaggtg ttggtcgccg aatcgcacca
    12601 gattatgtgc ctagaacgga ttgggactgg attatttatc ctgaaggctt gtatgaccaa
    12661 atcatgcgag tgaaaaatga ttatccaaac tacaagaaga tttacatcac tgaaaacggt
    12721 cttggataca aagatgagtt tgtagataat accgtttatg atgatggtcg tattgattac
    12781 gtgaagaaac acttggaagt tttatcagac gcaattgcgg atggtgcaaa tgttaaaggt
    12841 tacttcatct ggtcacttat ggatgtcttc tcatggtcaa acggatacga aaaacgttat
    12901 ggactattct atgtagactt tgacacgcaa gaacgctatc ctaagaaatc agctcattgg
    12961 tataagaaag tagcagaaac tcaagtgata gagtaatatt acttgaaaat gtaaaaaatt
    13021 gttatccgat actttagaaa aagtacaaca cttatttaga ccaggctttg aaaaggtata
    13081 tattgttagt ttcgaagatt gtcctatgat tcctgagctt gaagctaccc cgttgctaaa
    13141 atgtggtaaa tggtatgttt caactggaaa agagtggatt tgccattctg atttagagtt
    13201 atcagcattt gaatgggaat ttttacaatc actcgatgta gaaatacgtg agactattca
    13261 cttcgaggta aattatttgc ctttccaata ataaaaagaa aggaattagt tgtgactatc
    13321 gaattaaaaa acgagtatct tactgttcag tttaaaacac ttggtggtca actgacttcg
    13381 attaaagaca aggatgggat agagtatctc tggcaagctg atccaaatta ttggaatgga
    13441 caggcgccta ttctatttcc tatctgtggt agtttacgaa atgattgggc tatttataga
    13501 cctcaagaaa gacccttttt tacaggtctt attcgtagac atggttttgt tcgaaaagaa
    13561 gaatttattc tggaagaagt caatgataat agcgtgacct ttagtattaa accaaatgct
    13621 gagatgcttg ataattacct atatcagttt gaactgagag ttgtttatac cttgaatggt
    13681 aaatcaatta gaaccgaatt tcaggtaacg aacttggaaa tggaaaaaac aatgccttac
    13741 tttattggag cccatccagc attcaatagt cctttagtag aaggtgaaaa ctatgaggat
    13801 tattatattg aatttagtga ggttgagtct tgctcgatac caaagagttt tcctgagaca
    13861 ggattattag atttgcaaga tcggacacct tttttggaaa atcaaaaaat tctcgatttg
    13921 gactactcac ttttttccca tgatgccatt acacttgacc agctcaagtc tcgtagcgta
    13981 acgcttcggt caaggaagtc tggtaaaggt cttcgagtag attttgatga ttttccgaac
    14041 ttgattctgt ggtctacaac taataaaagt ccattcattg ctttggaacc ttggagtggt
    14101 ctctccactt cacttgaaga aggtaatttc ttagaagata aaagacaggt aacgaaaatt
    14161 tcacctcaag aaacttctag aaaaatctat gatattacta tccttaatta aaatttgtta
    14221 ttatttgagt cttcaataag atttgaatac tcttaccttg tgatgataga ttggtcttct
    14281 ccgtgccagt cttacatcgc aaggttttta agttttttgt gggtctatgt caactgtagt
    14341 gggtgacgaa aagctaatat ctaaagacag tctggtagta aaaaataaga ttatttacta
    14401 cttcttgatt taaaataata gtggagccat taatttgtgg caaatttaat ttaatatttt
    14461 gcctcaaatt agccacactt tttatagaac taagaggaaa aatgaaattt tcttcctatt
    14521 aatatagttg tttgaaatct taaatatagc gt
//
LOCUS       DACKAC010000068        14036 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.069, whole genome shotgun sequence.
ACCESSION   DACKAC010000068 DACKAC010000000
VERSION     DACKAC010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14036
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.069"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..1402
                     /locus_tag="I0B46_002203"
     CDS             <1..1402
                     /locus_tag="I0B46_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAR2084468.1"
                     /translation="EGEKVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTNTPG
                     NYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNL
                     PALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKY
                     NPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDG
                     SSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKE
                     KVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTE
                     PNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVS
                     SNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILL
                     GTLGLLGYKNRKKENE"
     gene            1536..2879
                     /locus_tag="I0B46_002204"
     CDS             1536..2879
                     /locus_tag="I0B46_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAR2084469.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            2991..4394
                     /locus_tag="I0B46_002205"
     CDS             2991..4394
                     /locus_tag="I0B46_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAR2084470.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTISS
                     TESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQSQPI
                     LSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQA
                     PNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGI
                     SGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGP
                     QMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSW
                     GNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDS
                     SLSQSMLVDPKTLLFGD"
     gene            4743..5375
                     /locus_tag="I0B46_002206"
     CDS             4743..5375
                     /locus_tag="I0B46_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAR2084471.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            5963..7402
                     /locus_tag="I0B46_002207"
     CDS             5963..7402
                     /locus_tag="I0B46_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAR2084472.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            7697..7915
                     /locus_tag="I0B46_002208"
                     /pseudo
     CDS             7697..7915
                     /locus_tag="I0B46_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            8164..8379
                     /locus_tag="I0B46_002209"
     CDS             8164..8379
                     /locus_tag="I0B46_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084473.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(8596..8852)
                     /locus_tag="I0B46_002210"
                     /pseudo
     CDS             complement(8596..8852)
                     /locus_tag="I0B46_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(9039..9950)
                     /locus_tag="I0B46_002211"
     CDS             complement(9039..9950)
                     /locus_tag="I0B46_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAR2084474.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(9969..10973)
                     /locus_tag="I0B46_002212"
     CDS             complement(9969..10973)
                     /locus_tag="I0B46_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAR2084475.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(10970..13093)
                     /locus_tag="I0B46_002213"
     CDS             complement(10970..13093)
                     /locus_tag="I0B46_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAR2084476.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(13098..14018)
                     /locus_tag="I0B46_002214"
     CDS             complement(13098..14018)
                     /locus_tag="I0B46_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAR2084477.1"
                     /translation="MPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILT
                     FLTGISSRDGEKFPVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTD
                     YDFAHLLFSDGSITQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVIS
                     TFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDD
                     LLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLY
                     GRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         4583 a         2492 c         2642 g         4319 t
ORIGIN      
        1 agaaggcgaa aaagtagaag ttgggggcac agtagatcta acagataatg ttactaactt
       61 gccagcgtta ccagaaggaa caacagtaac agatgttact cccggcggta caattgatac
      121 caacacacca ggtaattacg aaggtgtcat tgaagtaacg tatccagacg gtacgaaaga
      181 tacggtgaaa gttccagtag aagtgacaga caatcgttct gacgccgata aatatgaacc
      241 aacagtagaa ggcgaaaaag tagaaattgg aggcacagta gatttaacag ataatgttac
      301 taacttgcca gcgttaccag aaggaacaac agtaacagat gttactcccg gcggtacaat
      361 tgataccaac acaccaggca attatgaagg tgtcattgaa gtaacgtatc cagacggtac
      421 gaaagatacg gtgaaagttc cagtagaagt gacagacaat cgttctaatg cagataaata
      481 caatcctgaa ggacaaaaag tgactacaga cttaaataaa gagccagatg catctgaggg
      541 aattaagaac aaagaagatt taccaaaagg aactacttat gcttggaaag agaaagtaga
      601 tgttagcaca gctggaaata aaaaaggtac tattgtagtg acatatccag atggatcatc
      661 tgatgaagtt gaagtagacg ttacagtgac agatactcgt tccgatgcag ataaatataa
      721 tcctgaagga caaaaagtga ctacagactt aaataaagag ccagatgcat ctgagggaat
      781 taagaacaaa gaagatctac caaaaggaac tacttatact tggaaagaaa aagtagatgt
      841 tagtacagct ggaaacaaaa aaggtactgt agtagtaaca tatccagatg gatctaaaga
      901 agaagtagaa gttactattt ctgtagtaga taaaaaagca ccgaataaac ctcaagttga
      961 tcctattaca gagggtgacc aaattgttac tggtaaaact gaaccaaatg cagaggtgac
     1021 agttacatta ccagacggaa gtcaacacca tgggacagct gataaaaatg gtaattttac
     1081 agttaaagtt cctaaattag aggcagggac aaaagtgata gtaactgcaa ctgatgaatc
     1141 tgggaatact agtgaaccta ctaatgtagt tgtttcttcc aacaaaaaag acagtggaaa
     1201 aaacggtagt aaaggtagta agactgacaa tcaaggtagc aacagcaatc aagacaaaaa
     1261 tagaggcaaa agccagtcct caaaagtttt tcctaagacg ggagagagtg acagtaatat
     1321 ctttactatt agcggtggtt taatactgtt aggaacgtta ggattattag ggtataaaaa
     1381 tcgaaaaaaa gagaatgaat agaaaatcaa tttcttaagt cttctctgag aagaggaaga
     1441 atctttttct tcctcttttt gcttttttat gatgggaaat aagtgttagt taaagatgaa
     1501 tatacttttt atagaaagga aaagaatgta attaagtgga aaaaatagta attattggtt
     1561 caaaccattc agggctagcc gcagctcgat ttctaaactt aagcgattca aattatgaag
     1621 ttattctaat cgataaaaaa tcagaaatta gttacttgga atgtgggaca tcgttattta
     1681 taaaaggaga agtaagtaat ttagaagatc ttttttatca aaatagaatg acactaaatt
     1741 ctgagataaa aaagttattt ctagagacag aagttgacaa aattgatatt caaaataaaa
     1801 agattagttg tgtaaaaaaa gatggtacaa gattctctca agaatatgac aaagttatat
     1861 tagctacagg ttcaaagcaa attatgctag atataccggg gaataattta gctaatattt
     1921 tttcgataaa gaattttcca aatgcaaaag agatttttaa agctttggat gatagtacta
     1981 ttaaaaaagt aggaattatt ggagcaggat atattggagt tgaaattgct gaagccataa
     2041 gaaaaagagg gaaagaagta tatcttttcg atgttgcaga tagagtgttg tcgacttatt
     2101 acgaccgtag tttttcagat aaagttgaag aaatactatc aaaaaatgga atacatcttt
     2161 gtctatcaga aagtcctaaa aaatatttag gaagaaaaaa gattgaaaaa atagtcacta
     2221 caagtggtac ccaatactct atggatatgg tcgtacaagc tataggattt ctatctaata
     2281 gtccaattgg ggaaaaggaa ctcttgagag atgttagtgg agcatatcta actaatgaat
     2341 accagcaaac aaatataaaa gatatatatg ctattgggga ttgtgctaca acttttttta
     2401 cgtccattaa tagaagtagt gttgattttt ctatttctaa tgctctaaga acagcttata
     2461 ttgcaactca acatattaac aatcaagatt ttacaccttc tggttcccaa tttacaaatg
     2521 gcttttctat atttaattac aatttttacg cagcaggttt gaatttaaaa acagcacgat
     2581 tattaagtga gtctatagat tatatagaaa tagaagattt cattagacct gcttttttga
     2641 gaaaaaatag tcgtgtaaaa ttaagaatta gctttgaaaa aaatacaaag agaatagtag
     2701 gtgtacaact ttgtagtcaa gaagatttat ctggactatt atcaatgttt tctcttgcta
     2761 tagaagaaca aattacatta gacaaattga aaatgttaga ttatttattt catcctagtt
     2821 ttagccaacc ctataatttt attttaaagg cagtaacagg attaaacatt gaaacataat
     2881 cgttactaaa tgaaatcaag aaatatactg tattactcaa ttcattgaaa atatttatca
     2941 taatttttaa tagaaaacac tattaaagag aaaaaagaga ggtaaatcaa atgaaaaaat
     3001 ctttaaaact tgcatcagca gttatattaa tgtcccaact ccttatagca acacctttcc
     3061 atgtgttggc aagcgaaaat tcaacataca aaattgcaaa agaaagaacc gagacgactg
     3121 aaatgagtaa ttccgatgta acagcagaga gtagtgaact tttgacaact tcatcaagtg
     3181 aaagtagtac gacttcaaat gccgagtctt caacaacttc atcaagtgaa agtagtacga
     3241 cttcaaatgc cgagtcttca acaacttcgt caagtgaaag tagtacgatt tcaagtaccg
     3301 aatcttcaac aacttcaagt agtacaagtg aaataacttc ttcgaaagtg acaaaaccaa
     3361 accatataga atcttcaaaa aaggaagcgc caaaaacgga aggacaaaca aaaacttttt
     3421 cgacaccaca gcaaatagaa tcaacagaat ctttccaatc acaaccaata ctatctggta
     3481 atgaagaaat tcattttgac aaaaatgaat ctgtagaaaa ttttattatg aaaattggtg
     3541 agtctgcgag aaaaattggt cagaaatatg atttatatgc ttctgttatg attgcacaag
     3601 cgatcttaga atctgccagt ggtcaaagcc agttagctca ggcaccgaat tacaatttat
     3661 ttgggataaa aggaacttat aatggaaact ttgtcattat ggtaacaaac gaagatttgg
     3721 gtaatggaac gctttatacg acccaatcaa aatttagagt gtatgaaaac tatgaggaaa
     3781 gctttgaaga ttatgccaaa ttattaacta agggaattag tggaaataaa gatttttatg
     3841 caggagcatt aaaagctaat agtaaaacat atcgagaagc aactaaattc cttacaggac
     3901 ggtatgcaac agatacacaa tactatttga agctaaatga attaattaaa acctacgatt
     3961 taaccaacta tgataaagaa gttaaaggtc cacaaatgag taagactgga taccaagtgc
     4021 ctattcataa ttattcaata accagtttgt ttggaaatcg tggaaatgaa tttcaccgag
     4081 gacttgatat ggcagctaca cagggtgagc cgattcatgc agttaaagct ggtaaggttg
     4141 ttaaagcaga gtttcactac tcttggggaa attacgtggt gatacaacac gaagatggat
     4201 caaccgcgtt atatgctcac cagcaacaat acctagtaaa aactggagat aatgttttgc
     4261 aaggacaaat aattggctat gtaggaagta ctgggaatag tactggccca caccttcatt
     4321 tggaaatttg caaagatagt agtctttctc agagcatgtt agttgatccg aaaaccttat
     4381 tattcggaga ctaaaaaagg taatcaatct gacaaagtaa caagaaaatg gttggattct
     4441 tagacctttg atcaggatat tgagactgga attgatatct cgattacgat gggtttgaca
     4501 aataaaacaa gtccattatc gaatatcgag tgtattattg ccgtctttat gctcatattt
     4561 tgagcaggtt tcactagatg aaaacaattt atctatcttg aggatttcgt gtacgtatcg
     4621 ttcagtcttg tattgtaaat tagtcataaa atcagactaa gaaaaattgg aaatattttt
     4681 ttctgattta ttaaaagtta attgaaatta tatggtactt ctttcttggg agggtaggtg
     4741 cagtgaagga aagatatggt caccttactg ttgttgagga gattactcat aatggatata
     4801 aaaaatatcg ttgcctatgt gactgtggga atgaaacgat tgtatatcga tcaaacttac
     4861 tatcaggtag aaccaaaagt tgtggatgtg gaaattataa aaataggcat aaatataaaa
     4921 atttgacaaa taaaactttt ggtaagttag ttgcaatcaa gccaactaaa ctgcgaagtg
     4981 aaggaactgt tgtttgggaa tgtcaatgtc tgtgtgggag aaaaaagtta gtatcggcaa
     5041 agcgacttct aaaaggtgat gtaaaaagct gtggatgttt gaaatcaaaa aagcatcaca
     5101 ttgaaaataa aaaattcgga ttacttactg caaagtatcc tttggacaat aactgttcat
     5161 ggaaaacaaa atgggtttgt cattgtgagt gtggcagtat gtgtgagata agttatagta
     5221 acttattttt tggacatact caaagttgtg gatgtttaaa aaggcaagaa acaaggacat
     5281 gtgttgaagg gacagtaata gagtgtctaa cgtcaaaatt accgaaaaat aatacaagtg
     5341 gtgttaaggg agtttatcgt tctaaagaga aatagattgc gtatataact ttaaaaaaaa
     5401 agaggtacta tttaggatcg tatgattcta ttaagagtgc agctcaagct agaaaagatg
     5461 ctgaaaataa attgtttcaa cctttgattg acaaatatcg aggtaatcat taattattgt
     5521 ggataataat taacagtgat atataaacat ctataatata ttattcatac tattgatatt
     5581 tagtataatc ctatggaatt gaatatcata gcaatgtatt tgaaaagcaa attggaaaac
     5641 aaataggtgc aacaccataa atatgaaaaa attgggaaaa agtagaatgg aaatttgctt
     5701 acgtctcacg agatagctca cactattaat ctgaagaaca atgaagtcga cttttagaaa
     5761 ttgctagaag aaagtaaagc gaattatcac tagttactat agagtaagtt caaataaagc
     5821 atctccaact tacatggtta catgaggata tcaaatcgaa attactcaag cttcctatgg
     5881 ctagagtcgc gagaagttgt tgggcagtag acataggtta tattgttctt agctacacga
     5941 tcattagaaa ggaaatccta ttatgtatag ttatcaagaa gatgcaaaag tccaaaaaaa
     6001 tcgctggtgg attttgattg ctatatctat gtttaccttc atgtccacat tggatggcag
     6061 tattgtcaat attgcactac ctaccatctc agatgatatg ggcataccaa tgaatcaagc
     6121 ggaatgggta gtgtccgttt atctgatgat tgtctgttct tgtttgttgc tgtttggtaa
     6181 aattggcgat agccgtggga aaatcaaagt atttcggatt ggtacgctga tctttgtctt
     6241 gggatcgctg ttgtgtgggt tcaatcattc gctaggtatt ttattgttcg cacgggtggt
     6301 acaagccatt ggtgctagta tgactatggc tacaaatgcc ggaatcatca cggaagtctt
     6361 tcccatacat gaacgtggtc gggcattggg agcaataggt gcgtttgttt cattaggctc
     6421 gattgctgga ccaggtcttg gcggcttgat tttgtcccaa ttttcttggg cgtacatttt
     6481 ttggatcaat gtaccggtgg gaatcgtaac gatgttgatt gctgaaaaat tcttaccaaa
     6541 agatatcacc atgagcggcc atacaatcga tggagggggc tttgctgcca ttgcggctac
     6601 gattatcagc ttttttggag caatcttttt ggggcaagaa agtggctttt tccatccagt
     6661 cccccttgta ctgttcgtaa tttcagtgat tgcctttgtc agctttttaa aagttgaaaa
     6721 gaaagcagcc tctccattga tttctttcgg tatttttaag aacaagattt ttaccatgag
     6781 cttgattaca gccttgttga ttttttcttc caacttcttt gtcaatgtag tgattccttt
     6841 ttatctgcaa aatgctcgcg ggttgagtcc tagctatgct gggctgttga tgatggtctt
     6901 tcctttgatg atggtcgtcg gggcgccttt aagcggctac ctgaccgacc gggttggccc
     6961 ggagattttg gtattagtag gcttgggctt attgtcggta acccaggtca tgtatatgtt
     7021 tttgtcagtg gactctcctt tgtggctata cattgtcgct actgccatca tgggtgccgg
     7081 caatgccttg ttccagtccc ccaataatac gatggttatg agctcggtca ctatcgaaaa
     7141 cttgggactg gcaggctcga tgaattcctt tgcccgaaat ttggggatgg tcagcgggat
     7201 ttcgctttct acgacggtct tatatcaagc gatgtctgct aagatgggcc agcaagtaac
     7261 taactatatc gcaaatcggc cggatgtctt tttatatgga atgaagatta cctttttagg
     7321 cagctttgtt ttgtgttttg ttgccttttt cctgactatc tggcgtatca aaaaacaagg
     7381 aggtattcag aaaaatgtct gaaaaaacca ctgttgaaga atttttggag gcctgcgtat
     7441 gggtgagtgg gatttccatg gatgatggaa gatgaagtga acttctaaaa gccgaaaaac
     7501 tcaagtatta tcttcaaaat actagtgctc aatgagatgc ttgatttgat cacaagaaga
     7561 ctgcgaaagc cgctggcaac cgcaagctag gtatcccacc gatggactac gttttagagg
     7621 ctactggtta tgaatacgga gccaacgctc tcatctgcat aagaatacat cacccagatt
     7681 atgtaaagat tttcgatact tctgtcaaaa aatcattaag atagataaat gatttccgtc
     7741 tagtcaaatt ggttcagaat gtagacacac agatggtaag aagtcactca caattcgaga
     7801 atggacttgt cctatttgcc gtatacatca taatcgaaac gtcaacgata gtatcaatat
     7861 tttgaccaag ggctatgaat acaagcattg ccttagaaaa catcaagaac cttaggaaca
     7921 acggggatag cttggttaat aagagatacc gctgtttagg cttgcaagta tactctttta
     7981 tcaagaattc cgtggcttta gtcatgagag gcttaatact atgaaaattt tctttagcat
     8041 ttttgattta ggttcctttt ctaattataa taagatgacc actacagtaa cctactgaaa
     8101 tatataaaca gccactgtag taccattttc aaagtagtct atgagaggag aatcttcatt
     8161 cttatgaacg ctggaattgc ggatatgaat ttaataaaaa aaacccttaa tgattttact
     8221 tccaattcaa tatcaaaagg tactggtatc aatttaagca caataaaaaa gctgaaaagt
     8281 ggtgaaagat cagtggaaaa gctgaatctt ttagatgcta taaaaataac ggaatttgct
     8341 atgaaaaatg gaaaagctga gattgaaata tggagataat tatcgtctag tgaattactt
     8401 gccacttagc tgaatttaac gatccttggt gcttgaaggg ggagattctt gggaatagag
     8461 catacttgca agcctatgtc tttactaaca acgatatctc ttattaacca agctatcccc
     8521 gtagttctaa ggcttttact tttatctaag caataatcgt attcaaagga aatttttgac
     8581 ccttagaaag tctcatttta tcagacgtga gcttaggcat tgctgaattt tgttaattcg
     8641 gttttaggtt gtgctttgag cataatatga aagtattctt tatcacgatt tcattcatct
     8701 aaggtaatgt gatgtgattc tgaaattctt tcaaaggcag tttttgcata agctgacatt
     8761 acatcatcaa tgacatgttt acaatgtttc actactaaaa taagggggta accaagaaga
     8821 aaaactgaat gattatttgt atctaattcc atggtatatc aaccatttcc tatatagact
     8881 gattatgaca tggattaaaa taaaaaggca ctatgtctta attgtcggtt ttcgagttac
     8941 cgaatggcat tcatcaccca cctatagagg atgggcgact tctgcctgaa ataagttaca
     9001 ctacttataa cttctaataa aattagattg agtcattatt atttaacatc aacgattttc
     9061 cagtttccat ttttttgtaa tactaatttg tactgaaagt agctagtggt ttttgataca
     9121 ttatcaaggt attttatctt tgtactcact agaaaatttt ttccgttttg tagataaact
     9181 ggattctcta actccgaaaa ctttaagtca atattgattg gtcgcatcgc accattttca
     9241 acataatact ccaattcttt ttcagaagct gatggataaa gtttaaaaaa tgtttctaaa
     9301 aaatcggtga tttcttccgt tgttttggca tcaatttctg aatcgttttc tacttgtttt
     9361 gcttcatatt ttgccttgtc tggctttcca atcattgttg gcaaactagt tactaaatga
     9421 ttcctgtttt catcttcaaa gattgtcact cgatatgcag aagaaattga ttgcgtcttt
     9481 tttccctctg taatatgttg ctccactaaa aatgaaacga caaattcttc cgagtgtttt
     9541 tcaacatcta gaatttttac agattgaact tcggaactcg tgggaatatc tgctcgaacc
     9601 atatcttgga ttaaagcgag accgttttct gtaaggtact gttcgagatt actcattctc
     9661 ttttcaattg cttctttgtt atttttccat gaaaaatatt ctttcgcaaa atcctctgta
     9721 aaactttcaa cgccagaagt attcactacc ttctctttta taaccacttt ttcatgagtt
     9781 gtatgttgat caatggcggt gaaattttta taaacgccaa aagcaagact acaagacaat
     9841 aaaagccaaa gggcaagcac catttttcga tgcttaccca cggatacaac tcgttctttt
     9901 ttcttttttg ggctcttttc cttttgattc cgttcaattt ttatcttcat tctttttcct
     9961 cttttctctt atttgtatcg gccagctccg ataagatgct gccgccaata gttttccgtt
    10021 agattcgtcc accccactgg atctcctgca tgaaacatgc gattctcccc tgcgtaaatg
    10081 cccacatggg tcacataagt accggcatca taggttgaat gaaagaaaac taaatcacct
    10141 ggtttggcat cttttagatc aatatgagtc atggcatcat attgatcctg agccacccga
    10201 ggaagtttca ggtcaacttt ggcataacac cattgggtca agccactaca atcaaacccc
    10261 gtttctggac ttgagccacc aaacacatag gccgttcctt catacttaaa tgcctcatca
    10321 aaaatttctt ggactatttt gtcatcaaac tgagttgtag ttaggtattg cttcactaat
    10381 aaacagtaaa acatattgcc ataattatac cgccacccac cattttcctt aatcgcgatt
    10441 gggttggggt aatcgacttt ttgaccacct gatttttcct ttgaaaaaga ttcagctaaa
    10501 gcaaaactat gcttcttccc gtttttcccg acatgatcta gatacccgcc accatagtta
    10561 taggcctgaa taatactatc ttgatcacac cctagttctt tggaacgctt aaccaaagac
    10621 gcaaaataga gacagccttg tttgatggat gcctcggtac ttaatgaatt aggtggtaat
    10681 ccggctgact cagaactttg cataacgtct tccattgtgc caccagattc cacttgaata
    10741 atcgccaaaa gaattggaat atactctgtt atatccgatt ctttggcata cttttccacc
    10801 attgcacggt gtttcaatac ttcttctgag acactagttc cgccaggaga agtgccagaa
    10861 ctaccatttc cctcactgtc ttcaccaaac aacaaaccca cacacatcaa aatacccata
    10921 aaagagatgg tcagaaaagt taatgaaata acacccactt tcttcaagtt cattttttct
    10981 cacctgacct tgattttgga cgatggttca ttcttttttg tactttttga ttggtctgct
    11041 ttggcagagt ggacctctgt ttcacttgtt ggaaagaatt tttcttactt gctgattggc
    11101 ttttcccttt tgaaatgatt tcttgactag ttagttttcg tttgatttcg ggattcagat
    11161 ttgctctcac aatccgcttt ggtgcttctt ttttcagcat catatctttc tgaggttgag
    11221 ctgctttatc tattggaata ttcgcccttc tttttccctg acgatttgtt gcttcactca
    11281 gtatcttgcg tttttcgtcc attttcgcat ttcttcgttg tcgttgtttt tctcgcaaat
    11341 ccactcgatc cgctttttct tggaacagtc cgcgcttgaa ttcttctggt gcttttttcg
    11401 acttttcgat tcctttatgg agattgtatt tcaaattcgt tggcgtattc cttacctgat
    11461 cctttacttg tttagtctta tctttcacca aagcttgggt atccaaaact ttgccgattt
    11521 ttctcccagc caaattcatt cgggattgtt tcttattatt tgaaactgga ttctccttag
    11581 ttaagtcaga tagtatttcg tgatcgttcg gtaatcgtga attctttcgt agagaagaac
    11641 cggatggctt aaatttggat tttgattgtt cctttgccaa cttatttccg actgctgcac
    11701 caccaccaaa agccaaagct cgtcccacat ttcgattgag ttgccgcaat tttcgattca
    11761 ttaacatttg cggttttcgc atgacgcgtc ttcccattga ctggctatca ttactctgga
    11821 ggttgaacat actcataatg tcgcccaatt tgaaatagat accggcaaag gtcacaattt
    11881 gcagaaaagc aaccatgaaa aatggatagg ttgccgaaat attgaaaaac atcgtggaga
    11941 tgctaaatgc agtcgtaatc accaaagtaa cacccgctcg catcataatc gtattgaata
    12001 aacgaataat cgccttcttt cctaaacttt cataagtcgg caacatggac aataagaaac
    12061 taattggcag aaacatcgca aagataatga agagaatttg cgagaacaac ataatccctg
    12121 ttagaagaaa aacaaagaag gaaatgccaa tattgaacaa taccaaaaag accacggtcc
    12181 ctaaacggtt catggtcttg gtaatactga gattcgcatt gtcattgtct tcaatctctg
    12241 ccttgaccgc ttcttcccga tctttgcctt tgttttcatc tggactcaca gatagaatct
    12301 tgttgacccg gtcttcacca atttcttcta tgttggaatc atcaaattgc agtagtaacc
    12361 aaggttgttg gacttgaatc gaaaacaaac tatcccgaat caaatccaca ctatcttttc
    12421 cttggctttc tgaattgggt acaacgatct cggttccaag ggtaagggcc gcttcactaa
    12481 catctgagct aaagtcattg atttttgtga tataggtagg cgcataagca atgaacgaac
    12541 ccgatagtag aaaaatcatc acaaaattaa tcacggctcg aactgctttc gtggtttctc
    12601 gttttaataa tccggtatac gccacataca cccccagagc taaaatcatc aataatagaa
    12661 acccaaaata aaatccggag gtctggagtc cattttcagt aataccagcc aatgtttgga
    12721 tattctttcc aatactttca gccgtatccg aaatgaaatc taatttgtaa gcctcttgaa
    12781 ccacataacc cgtggcgttc gacaaataca aactcaccgt ccaaatgaaa ttagtaatgg
    12841 cataaagacc atacatcacg cttttcccta agccatcacc ccaattccaa ggcaaccaat
    12901 cccaagagct atccacaaag aaatctagtt gatagttgtt cagtgaatat ttggaatata
    12961 gatttcctgc ttcaactgta tcatcaacta gccctgtcgc ctcggctacc gtaccaacta
    13021 aactcaatag aactatcaga gtgagtatgc taataaaaac gattcccaca atccgaaaaa
    13081 gtttcttttt catcccctca ctccattctc tcttgaacag gtggacgggt atcaaaggca
    13141 tggaagagtt cttcaaagac tggatgtacc tgaaccacgc ctacccgacc atacaaatcc
    13201 tgcatcaagc attggccatt ttccaaatct cgtaggcgtt tttgattgct ttcatcttct
    13261 cgatccaagc caaagaattc caaggttttc ttgatttcat gaatatcggt actgcgaaac
    13321 gcaaacttca acccgatatt attcttcaag cgttcatcca gtaaatcatc actattttgc
    13381 gtgacaaagt aaaccccggc attcatagcc cgcccagcac gaacgagctt attcgataat
    13441 gttttccctt gcgctacttg aagaaaactc cacgcctcat ctaagtctac aattttgaag
    13501 atttctcgat tactatggat aaagtccaag gcaaaagtgg aaatgacgat tagcatggaa
    13561 acactaagca attccatggt ggtatattct tcaaaagtgg tttctgcatc tggtaaaacc
    13621 aaatccgcta cttgaatgat atttaactgc ctatctaaac taatcgactg ggtaatcgaa
    13681 ccatctgaga aaagcaaatg ggcaaaatca taatcggtaa aggactcgat gtgatccgcg
    13741 atattttccg caactggtga gccttctttt cgaagctcat taattacttg taataatccc
    13801 cgttgattgc tttgggtaac cgtccgaatg gccttacgca acactggaaa cttctcccca
    13861 tctcgactag aaattcctgt taaaaaggtc agaatatcaa ttgctagact ttcagaatcc
    13921 ttctttttct ccataatcac ataaggatca agtaatcctt gattttttgg cttactagtt
    13981 aagttgagaa tattgatttc atccgcaatt tctggcaagg tttctttcca gccacc
//
LOCUS       DACKAC010000069        13946 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.070, whole genome shotgun sequence.
ACCESSION   DACKAC010000069 DACKAC010000000
VERSION     DACKAC010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13946
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.070"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            948..1421
                     /locus_tag="I0B46_002215"
     CDS             948..1421
                     /locus_tag="I0B46_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAR2084478.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1430..1657
                     /locus_tag="I0B46_002216"
     CDS             1430..1657
                     /locus_tag="I0B46_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAR2084479.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            2293..2664
                     /locus_tag="I0B46_002217"
     CDS             2293..2664
                     /locus_tag="I0B46_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084480.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2920..3162)
                     /locus_tag="I0B46_002218"
     CDS             complement(2920..3162)
                     /locus_tag="I0B46_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAR2084481.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(3194..4096)
                     /locus_tag="I0B46_002219"
     CDS             complement(3194..4096)
                     /locus_tag="I0B46_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAR2084482.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(4109..4297)
                     /locus_tag="I0B46_002220"
     CDS             complement(4109..4297)
                     /locus_tag="I0B46_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAR2084483.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(4311..4874)
                     /gene="amaP"
                     /locus_tag="I0B46_002221"
     CDS             complement(4311..4874)
                     /gene="amaP"
                     /locus_tag="I0B46_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAR2084484.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4912..5805)
                     /locus_tag="I0B46_002222"
     CDS             complement(4912..5805)
                     /locus_tag="I0B46_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAR2084485.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5883..6821)
                     /locus_tag="I0B46_002223"
     CDS             complement(5883..6821)
                     /locus_tag="I0B46_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAR2084486.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(6855..7205)
                     /locus_tag="I0B46_002224"
     CDS             complement(6855..7205)
                     /locus_tag="I0B46_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAR2084487.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(7238..8140)
                     /locus_tag="I0B46_002225"
     CDS             complement(7238..8140)
                     /locus_tag="I0B46_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAR2084488.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(8133..8990)
                     /locus_tag="I0B46_002226"
     CDS             complement(8133..8990)
                     /locus_tag="I0B46_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAR2084489.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            9324..10133
                     /locus_tag="I0B46_002227"
     CDS             9324..10133
                     /locus_tag="I0B46_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAR2084490.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            10173..10670
                     /locus_tag="I0B46_002228"
     CDS             10173..10670
                     /locus_tag="I0B46_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAR2084491.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(11055..11128)
                     /locus_tag="I0B46_002229"
     tRNA            complement(11055..11128)
                     /locus_tag="I0B46_002229"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11092..11094),aa:Ile,seq:cat)
     gene            11315..11638
                     /locus_tag="I0B46_002230"
     CDS             11315..11638
                     /locus_tag="I0B46_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084492.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            11802..12056
                     /locus_tag="I0B46_002231"
     CDS             11802..12056
                     /locus_tag="I0B46_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084493.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            12126..12371
                     /locus_tag="I0B46_002232"
     CDS             12126..12371
                     /locus_tag="I0B46_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAR2084494.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            12447..12812
                     /locus_tag="I0B46_002233"
     CDS             12447..12812
                     /locus_tag="I0B46_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084495.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(12873..13436)
                     /locus_tag="I0B46_002234"
     CDS             complement(12873..13436)
                     /locus_tag="I0B46_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084496.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
BASE COUNT         4089 a         2577 c         2304 g         4976 t
ORIGIN      
        1 tttcggagaa ttaaaaaata caataaataa ctttagttac tttaatgcgt tcttacttta
       61 tttttatatt atcatttttc ttattcactt ctttggttta tactattctt ataaataaaa
      121 acgaggtatg tcctatgatg aaaagattgt actattcatt gatcataacg attggctatt
      181 tgattgtatc aaatctagga aatatggtat ttggtatcag taaagaattc agttggacta
      241 ctacattatg ggaatctcta ttctttttca tcttcgtatt tttgttacaa aattatcgaa
      301 aaaaataaac atgtgccatc tatctgttgg cacatgtttt aggatttatg gtacttttta
      361 ttcggcaatc cattttattt ttctacttct tcttaatacc tctaacactt ttgatttata
      421 aactaatcca atgcatctac ttgcgcttat taagctattt cagctagata gtaccacccc
      481 atgcgtcaat gcttgatttt ccttttgtat aacattgtat aagcaataaa ggacaaataa
      541 gtggtgatta caccaaacca ttgaataaga aaaattctga tacgtaaata aacatgaact
      601 tgaaaaaatt aatgaatcgg ttagctacta cgtagttgtc ttttttctca tacagttctg
      661 ttaacttaca cttctcttcg ctagataata aaaaatcctc caactgtatt gtccaatata
      721 atcccaggca tctaaactag cattatagaa gagacacaca tagaatctaa aataactggt
      781 tttaaatgtt tcttacaatc aaaaaaagct gttaaatgca ctccacacct tcatactcta
      841 tcatttttta taaacgatat atttttattg tttttcgata aaaaatagtt gttttacaat
      901 aattattctg ctatatttta gctatcaaag ataaaagagg gataaaaatg aaatttaaaa
      961 cattgctatt aaaatttgtc gttttaggta taagtgccgt atttattttg ttcggcgtac
     1021 ttttttatac tcagatcgct acaagcgaaa aaggattcaa ttttgattgg cagaccacat
     1081 tatttgtttc tgtcatttac tttgcccttc tcctagggat cgttattgct tatttcttat
     1141 tgaaactatt gaaaaaaatc gaagataacc aagctttttc cactcaaagc ttaaacatac
     1201 taaaaaaaat cgaacgaacc attttgttta ttggtttcgc cttttttggc ttacttccta
     1261 aatttatgaa agtagcggat ttagacgatg ctccaggttt gatgttggta ggaatcgctg
     1321 tcgtattcat tccttttgca gtatacacat taacaagcgt gctggaaaaa ctgcttgaac
     1381 atgcgatttt acttaagaca gatcatgatt tgacagtgtg aggtgcgaca tggcaattat
     1441 cgttaattta gatgtagtat tagctcaaaa aaaaatgagt gtgactgaat tgagtgagaa
     1501 agtgggcatt acgatggcaa acatctctat tctaaaaaat ggaaaagcaa aagccatccg
     1561 tttttccact ctcgaaaaaa tctgccaagt tttggagtgt caaccaggtg atttgttgac
     1621 ctataaaaat gactcgaata ctataccaga agaataaaat aaagtagaac aaacagtaaa
     1681 taaaaaataa tctgaatgat agatgtgtga gacttttaga agttatttac gtctattatt
     1741 cagatttttt atttcgattg aataccgttt tatctataaa taagatatgt actgactcat
     1801 acaaaggggg ccttttcttc tccatgtact atcacttttc ctgatacaac tggtttcctg
     1861 ttacgttgta gttggatctg cttttagtat aatactttat tcattccacc agactgatag
     1921 cagtttgagc agtctaaata atcatgtagt attacaggcc attctgtttc ataccttgaa
     1981 gtgcttaaaa cttcagctaa tggcccattg tgttaactct tactccattc ttttttagta
     2041 agctcccata tatactgtaa agccttctat atattatgtc atgttgcttt ttaacattta
     2101 ctatgtagtt ttgataaaat gatttgaata acagtgatgg acaattcttt tttattttta
     2161 acattatttt gataataatg attatttatt taaaaataca ttagataaca tttgttacga
     2221 cacgaaaaag cttcacaaac aaatatcgtt attttaaaat gagggcgaac tcacacaatt
     2281 aggaggaata gtatgccatt tattgtttac ttttttcttt cattactcgt gatctatatt
     2341 ccattaccaa catttttcct gctcgtcaat cgtctagcag agcaacatga tacgattgct
     2401 gctcttacga aactttgttt acttctattt gtttgtctag attactttac gagctattat
     2461 tggctaaaac agacaagagt aaaaaagaga tattatttct tccttgtctt tttcaatctc
     2521 tttgagtttc tgatccattg ctttttactt catcagtatc atacgatcca tttgtggatc
     2581 atctgggttt tcaaagtggt cgtattattt tgtatgttca cctttcctat ctcaagaaaa
     2641 tttagggagg cattgtttta ttaaagaagc ttctctgtat caataaagac acatttaagg
     2701 aaaaactttt aaaaatctaa ttgggaattt gcccgcataa ataagaggct gtgacaaaag
     2761 tcttgtcaca gcctcttatt ccgtataaac agtgttcaaa agcttgcatc ttcgataata
     2821 tgcccaacca aatgaaaaat atgagaagct atttttgttt ggctgttctt attacttgat
     2881 gaagaacagc tttttcacga cctcttttta aaagtctttt tacagttttc gagcaaagaa
     2941 tgatacgatt agtactaaaa tgatggcacc aatcaatgat ggcacgattg ccattcctgc
     3001 aatacttggt ccccaagaac ctaataatgc ttgaccaagt gctgatccaa ctaaacctgc
     3061 tacgacattt gcaataatcc ccatcgaaga acctttattc gtgattgctc ctgcaattgc
     3121 tccgataatt cctccgacga taagtgacca aataaatccc atgtttcgtt cctcctaatg
     3181 ttttacttcc ttattactga acccgtggtt ctgtattttc ttttactttt tgtgcgccgc
     3241 ttccagctgc tgattttact ttatctgttt gattcgaagc aaattcaccg gtttttttcg
     3301 ctgcgtcagt tactttgtct tgcaccgttt cttgatcttt ttcaaattct tcttttgttt
     3361 tgatgtccac tacgttagcg ttgacttcga tcacttctaa atgtgtcata ttttttactt
     3421 ctttgccgat tacttcttgc atttcttcaa agattttttc gatatctttg ccatattcca
     3481 caatgacatc catatcaact gctacttgtt ttttaccgac ttctgtttcg atcccagccg
     3541 ttacattatc tgtattcaca agttttcctg ccacattaga gaagaaacca ccgtttgcag
     3601 ataataaacc gtctacttgt tcaattgcga ttccgatgat tttttgcaca actttatctt
     3661 cataattcag ctcaccgcgc attgaatctt cctgttcaga atcatgcttt ccttggttat
     3721 ttgtatgatc agaaccaagt tttccttgtc cgtttgtgtg gtcagaacta agtttttctt
     3781 gtgctttcgc atggtcagaa ggatgttttt gctcttccac aggtttagaa ggatcgattg
     3841 catcatgttg ttctggaact ttagattctg gagttcctgg atgatgaggt gcatgctttt
     3901 ctgcatcatg tttcttctct tctactcctt gttgaacgtt agggactact ggatctggat
     3961 gtgcagatac tggtgctgtt ttgttctttt catcgttcac accatttgat tggtttggtg
     4021 tcgttgggtc tggatgtgca gacactggtt tttggttgtt cattttattg ttattatttt
     4081 tgttattgtt attcatggat gtttcctcct aagatttaaa atagtgatct aagattttcg
     4141 tttgtttcag ataaaaaccg atagcgacgc cgatcgctgc catgatcaag atcaataagg
     4201 tcttgaaaaa gcccacggtg atccataaaa cggctaatat caatccaagt accccaaaga
     4261 tgatagggag cttgtatgtc gaaaatagtt ctttcatttt tggtcccccc ctattctact
     4321 cgtttgcggt cattgcttcg tttagctggt tcttcaaagc ctgtgtattt caccgatact
     4381 ttcactggct cttttgttcc caatatgtgt tgtacttcat tttcgatttc tttcatcaat
     4441 gttcctgttt taccaatgag tgctgaagtt cgtttcaact ccccttttac attgacggcg
     4501 atcttgtttt tcgttgcttt gacatttact tttggtgaat ggatgaattc atcttcatgc
     4561 aagtgagaac gaaccattcc ttcaattgct tttttatcca acgtcaactc cccatcacca
     4621 tgtttcagta gaaagtttcc gattgttttt ggataaaaca ggaaaacaag taacgtgata
     4681 attgtcaaaa tagcaaatgc aacggcaagc caaaatacat attgttgcac gtatgaatta
     4741 gtgatggaaa agaatcgaaa cttctctagc tgaaaaggca atggataggt tgcacttaat
     4801 agtatcgcaa acaaaagcac agatagcaaa agtaatgttc cgatcactcc gatcgttttt
     4861 acacctttat tcattttcgc acctcctcat aggggtgaaa cgatcaactt cttataagtt
     4921 gatcgcttct ccaccagtta ctccgtaaat ttgggctgta acataacttg ctttattcga
     4981 tgctaagaaa acataaactg gtgctaattc tcctggctga cctgcacgtt ctagtaaact
     5041 ttgctgacca aattcaggga tcgaatcaga aggttgtcct tcgtctagtt gtaatggtgt
     5101 ccagattgga ccaggtgcta caccatttac acggattcct ttaggtgcaa gctgttttgc
     5161 taaaccgatc gtaaagttag cgattgaagc ttttgttgct gcgtaatcta ataaatgttc
     5221 gcttggattg aatgcttgaa cagaagttgt tgttacgatt gctcctcctg ctgggatatg
     5281 cggaacagct tctttcacta aagcaaacat agcgatgatg tttatcataa atgtgtcttt
     5341 gacttgctca agtggtaact cagcaataga ctgacaagaa atttgctgtc ctgcgtttag
     5401 taccatcgta tctagcccgc caagttcact cacggcttta tctatgattt ctttaggtgc
     5461 ttgttcgtca cgaagatcaa aaggtagtaa gacaatctta cgtcctgcat ctttcacgta
     5521 ttttgctact tcctctgcat ctttttcttc tcctgggaag aattgaagag cgatatctgc
     5581 cccttctcta gcaaatgcaa tcgctgctgc acgaccgatc cctgaatctc caccagtgat
     5641 tagtacacgg cggttttcta gttgattatt gccttcatag gattcttctc cacaatcagg
     5701 aacaggaatc atattctgct gtaaagctgg tgtatcctga tcttgtttag gaaatttctc
     5761 cgtatggtac aagtttcgcg ggtcgattaa ttttggttct gtcattgctg tattcctcct
     5821 agtttttaaa ttgtattgcc aaaacaaatt tctttgtttg atacctgttt taaatagatt
     5881 ctttaatttg tataatcttt ccggtataaa tggtaaccta aaactactgc tgctatcaaa
     5941 gagccaaaga ctacgagtat aaagcctaga gcagaacttt tacccggtaa tcctcctgta
     6001 ttcattcccc aaatccctgc gaacaacgcc ggaacggaaa tgattaatgc agctgaatct
     6061 agatatttca tgatatgatt tagattattg tccatcatcc cgttgaatag atcaccaata
     6121 ctctctaata aatctcgata gatctcgatc attttttctg tttgtcgctg tctcaaatgg
     6181 acatcataga gtagctgttc atcgtctaaa cgtttgatga aagtttcaga attccataat
     6241 tgatccattg tttcccgttg atctcttaag gtatgatcga tatatacgat gtctctttca
     6301 agatctgcct gtttatacag ttcttcattt tccgttgttt tacgagctgc tttatccaaa
     6361 gcgtcaattc gctttttcat ctctcttagt tctttcacgt aatgggtgta aatatctaat
     6421 aatgtatacg tgatgatctt ttcaaacgag tttaaatgat ttccatgctt tgtcagcagc
     6481 cgatccacaa attcggtttt tctatcgata caagtaatca agagattatc ggaaagtaca
     6541 aaagatattg gcgttaattt tttttcgata cgattatctg actgatctag attaatcact
     6601 accaaagaaa atggatgctc tagtttcgtc tcataaaggc gctccatccg agaaacttct
     6661 tccggatatt cggttcctac gaaaatatca tcaggaagct cgaattcttc gattgcttgc
     6721 tcaatctctt cgggctttgc actatcaagt acgagccaat tatatttttt ttcatctgtt
     6781 tctattacat tcttttcttc atccacaaag tagtatctca atgtatacct cctttttgga
     6841 ctttctcgaa actattagct gaagaagcag ttctttaatt tgatattata atcgagtact
     6901 gcgatttcat tttctaatga aaagctgtca agctgtttgt tgaagtccat gttcaatcgt
     6961 ttaccgaaat aattttgctc taatttagat gtatagatgt ccaataatgg atgctcgatt
     7021 ttagtcgcat attctccaaa ttccgaaaca aaaggaatga tttgaaaaaa gctgcctttt
     7081 gcaaaagcac ctttgtccct tagaaatgga tcggaatcat tgataagcgc gagtgcgatt
     7141 tttgtatttt ccccaacctt ttcaattaac gtctctactg catttttaga cattgtaatt
     7201 tccattccta ctccccctct tcagttgttg tcttctatta tttaacaact acatctgttt
     7261 ttggttgaac ttttgcatat tttggatatt tagggaattt agctacacaa cggaaagccg
     7321 tcatccctgc atatttttcg taatatttct tcgttggccc ttcaaaatcc ttgctccacg
     7381 gtttcacatg tcctgaaaaa tggataatgt aaggattgtt gcgagtttct tcgtattcac
     7441 gttcacctgc tgctgttgga tgtttttttg cttttgccat gatatagcct tgtgcattcc
     7501 aacgcggatg aagtggtaac caacggtcat gtaagatagc atttaatgca tcttggtcat
     7561 ggaatcgtaa tttttctgga ttatgttcga tgaaatctaa tactttttgc gtaatatttt
     7621 catctaacca ttttttcaca ttgatcagca tcaaacctga gttgaaataa cgcattgatt
     7681 tagctgaaat tttcattttt tccagtcgtt gatggaaccc agcgtcttca acagctgcta
     7741 cgattgaatc accgaagtca agacgccata atttagagat atcttgtaaa gcgatcatgt
     7801 cgcaatccat atataagata cgttcaactt ctgtgccgcg gaacaattcc ggaatcgcaa
     7861 tacgataata agctgtttct ggaatacgat cgctgattac cacatttgta aagaattctt
     7921 tattgatttt caagaattcc acatctgagt tcattcgtgc gtttttcact gagaagcgta
     7981 aaagctgttt actttcaaaa tcgatatcat cgtcaatcac atagaaccga acaaatttag
     8041 acgggttgca attatctaat actgagacaa ataatgcagc taaatgtggt acgaattttg
     8101 tgttacaact tgcgactact gctatttctt ttttattcat tgatctcgcc tactttcgtt
     8161 agagtactgt gtgctaattt ttttaagtat aaattggtat acggatgatc tttcaacgta
     8221 ttccaaggtt tatcatgtcc tgtaaagtga acgatcgaag gtttttcatt tccttcttta
     8281 tactgacgtt catatttttc atttggtgct ggatgacgtt caaaaatcag cgaagtctgc
     8341 atgttccatt ttggatgaag ttgttcccaa tcttcataaa gtacagcatt caaagcatct
     8401 tggtcgtgat agatgattcg atcgccattt tcactgaggt aatggatagt tttttctgta
     8461 atttcttttt tattccattg atcaatgtca atcaccatca ctcctgaatt gaagtacaag
     8521 tcatcggaat cgattcctaa tctttccaaa gctttcactt ggcctggatc gataatagcg
     8581 ccgattgttt ttccatttaa cggctcatca tatagtttta cgatatcgtc tagtactaac
     8641 acatctgaat ctaagtaaag gactttctta taatcctctt tagctaataa gtttggtaaa
     8701 gaaattctta agtaagcggt agttgtgata tggtcactga ctaggaagtc ttcgtagata
     8761 tctttttcca ctgtcaggaa ttggatactt gcattacttg aatatttatt taccgtttct
     8821 tccaatcctt ctttactata ttctgataga ccatcgtcaa tcacataaaa ttttatgcgt
     8881 cttgttttat tgcaattttc taatgcagtt gcgatcatca cgctcaagta aggagcataa
     8941 ttttcgtctg aagcagttac cacaggaact acgccgtatc tcttctccat caaaatcact
     9001 cctttttcta atctttttct aacctgttct acatctagaa tattcctcat ttggttttaa
     9061 agcaaaatat atgctttgtt ttcattgaat attttaaaat ttttttatca caaaacgcat
     9121 cggtaagcgc taactattcg tttttcatgg ctttttattc aaaggtattg accaaaaact
     9181 actatatata gccacctttt tcttaagaat gttttttgaa aaaattcata atgacaaagc
     9241 aagttagttt cattttttta aattctcatt taatcttaag gtaacaggaa aatagatatg
     9301 taaaaagaaa gtaggaagat aaaatgaaac tagcagcgat tgatttagac ggtaccttgc
     9361 ttgatcgaaa tggaaagata ccagaagaaa atatccaagc acttcggaaa tttgatgaaa
     9421 atggcggaat cgtaaccgtc gctaccggaa ggaacagcat ttctgctaaa gatatttttt
     9481 ccgaattagg aatcggtggc tatttgattt cttccaatgg ttcactgatt gctgaaatga
     9541 aggatggaaa aatggcgaaa gttctcagac gttcaaaaat agatagcgat acactgagaa
     9601 aagccttctt ctatgctcaa gaagcgaaaa tatccatcat tgctagccga gaaacccaag
     9661 atgatcagct gacattcgat gaatcaaatc tagtcaaaga tgacccttat tatgaacatt
     9721 tcaatttaca gaaccattct tttgatcaaa ttttaaatat gttggaagat ccttctctta
     9781 gctatcttaa attagcatta acagataaga gtgaagaaaa actacagaag ctacaaagag
     9841 atctgcaagc agatggtatc caatcagtat tctctgatcc gcatttctta gaaatcacgc
     9901 ctacaggtat cactaaagct cattcacttg tttttctggc ggattacctt ggaatcgaat
     9961 cgagtgacat catggctttt ggcgatcaag aaaacgacat tgctatgttg aattacagcg
    10021 ggttgagtat tgcgatgggg aatgcacagc gacaagtaaa agaaattgca aaccaagtaa
    10081 cactgacgaa tgaagaagct ggtgttgctt atattttaaa aaacttctat taaaaagaat
    10141 aaaatgacga tatgaatgta ggaggaaaga aaatgttagt tattattgat atgcaaaatc
    10201 atattcttga tccaaacagc gaattttata ttccagagtc agatcaattg gcagaacgta
    10261 ttgctcaaag attagctaaa gcacgccaaa ataatgaata tattctcttc acacgggata
    10321 taccagttga actgaaggac aaagaagaag aacgagaaga tttgcaattg atccctcttc
    10381 tttctccaca agaaaatgaa cgagtgatta aaaagtatta ctttacgatt ccacctgaaa
    10441 ctttagctga aatccaggaa actttatttg aaagcaaaga agaacaaaaa gagatcgaag
    10501 tagtcgggat tgaaacaaac ttgtgtgtac tctctaatct gataggcctg cagagtgctt
    10561 ttcctgaagc agactttttt gttgatccaa tgttagtctc cagtagaaaa cacggagaat
    10621 ccgctttaga gctgttgaaa gatttcaacg tctctatcgt cgaatcataa aaatatctat
    10681 cttcatttac gatatttaac acacaataaa aaaagcacgc agaactgttt tagtcctacg
    10741 tgctttttct atcaaagtag attggctttt ttatgacaca gagtgaaacg gcaggctcac
    10801 gacctctttc ttaaatggtg ttcaatcagc tgaccctcgg tattagctta acaaacggca
    10861 aaatatgagg aactattttt ccgtttgtta agctaatact caggtcaaaa cgctgatttc
    10921 acaacctctt ccttaaacgg tattcaaaag cttgcatctg cagtaataag aacagccaag
    10981 taaaaacata agaagctgtt tttacttggc tgttcttatt acttgatgca gggcagcttt
    11041 ttcacaacct cttatgtacc ttgtaggact cgaacctacg accggacggt tatgagccgt
    11101 ctgctctaac caactgagct aaaggtacat cataaataaa atttattttt gttacttttc
    11161 aactatagca cgaatgacaa actctcacaa gtttcaaaat ataagtaaaa taatattgtt
    11221 attcttattt cttttacttg tgtcataact attttgttac tttaattttt atttgcttat
    11281 tttagaaaaa atgttattct tttatttatt ctcaatgtta ggagtgatta ttttgccatt
    11341 tattgcgatt ttattcgatt tagttgcggc agcagcttat tttctccaat taaattatca
    11401 aacctcagga attctgttta ttggaatgat ttttcaaggt gtgattaccc ttttgcttct
    11461 tgtcatgatg ctcagctata aaggaaaaag atatgcccgt attcaaacaa aagtatttgt
    11521 taaatatgtt tccattcgct atgggatcat cattctttcc tttttagtga atgcgatcgt
    11581 cctcttttta tatgtattga attacttaga tatcaatcca ttgatttttt ctcgatagat
    11641 aagacacatt cagcggaaca gttcctgctt atcagcagaa aactgttctg ctttttcatt
    11701 tttcattgat ctattcttct tttattttcc tagcatttca tttgtgatgt atgcaataac
    11761 atgttatctt ttattatatg aatcgttgag gagtccgtta catgattacg ttggtgagag
    11821 ttttgttttg ggtgcctgcg gtcgcactgg tcgcaagtat tgtttatctt atgaattgga
    11881 acaaagaacg gttttattta gctattttga ctcttccggc tatctacttt atgtggaaag
    11941 tttttaatta caactacttt gaacccgata gtgtttttat agaagaatta tcaggactgg
    12001 ttttgtcttt attgatcgtc attctttatt tgatacgttt aaataaaaaa cattgagtac
    12061 tattgattgc atgtgtagct ttctatagtc attataacta ttctactata atgaaaggtg
    12121 attaaatgag tatcgaagaa gctattttac tggatacttt acgttgtcgt ctgaatttct
    12181 taagaactgg ccgatcttta tctcaagtag ctgtttctag cgcgttaagt attcctttat
    12241 tcaaatatcg taagtacgaa gaaggagaag aaattccaag catcattgaa ttaatctata
    12301 tcgctgactt ttacgatatt tccgttgatt acctaattgg tcgctcagaa acagcaagca
    12361 aactgaagtg attttttcca gccttggtgc tggttttttc atttaacgtc taaggttaac
    12421 aatctatata taaaggagac atttctatgc gattatggca tgaaaaattg atttcaaagc
    12481 ttcctcgtca acagctttta ggacaacatc gggaatgctg tgcattaaga ggaaatggct
    12541 ggggaaagaa acatgctact gtggattatg tttttcacta ttctccttat agattattcc
    12601 aatatcatca gcttgtcatg gatgaaatgg aaaaacgtgg ttacagccca gcaccagagt
    12661 ggaaaaatcc tatgtatcgt ggcaaatcat gcgaagcatt ttttgattta cctactgtag
    12721 agcgcaacgt gccgatttat ccggaacacg atgaagttta tctagcagaa tgtttagcaa
    12781 atttggagca aaaaggaatt tatcttacct aatataacta gaaaagcctt ctatctcctc
    12841 ttacttcatt aaaaagcttt aaccatctta gatcaagcta aaatgtcgta agttttaggg
    12901 ttatcttcat cctcacgtgc aaaactaaaa gcgtactcta gttttggttc atcgccaact
    12961 tccctagaca taaatgaaca tctcctgacg tggtttaaaa gtaccgcatc ttcgacgtct
    13021 gtatacgatg cacagcacag attgcttctt ttgatctttc ttttgatttt tctgttgctt
    13081 cttctacacc ttcttcgatt actacatcga tgcttacaat tgcttctgcc taatattccc
    13141 atgcatcgtc gatttaagcc tctgtagtca ctgtgatcgt ttccacattt taactcctct
    13201 gtcgtggctt cagttgattt tgtgatcagt tcagttgcta agccagagtc agaacaacct
    13261 gccattgcta gcgttaatat tgatagtaaa aatagttttt ttatcataag gttccccccc
    13321 tgaaaataga ctaaaataat aacgctttct ttttttgttt tcagacgaaa attaataatt
    13381 tctaaatatt ctttaaaaaa gcttataact attatgcctt ttttaatata taacacagtt
    13441 atttttaaaa ataagaatgc taaatcactc tacaataacg gcgaaagcac aaaaaataaa
    13501 aaaattatta cgttattaaa aaaacagatg aaagatcagg ttttattaga tctcattctt
    13561 tcatcacttg aagggattta atgaactact ttttcctctt tgtcgttttt atatattcgt
    13621 attccttcaa acagtaggat agcctctttc aatgcttgat atttgaactg tccgttactt
    13681 aaaaatttag atgtgccgcc ctcttcctag aacatattct tttttacttt ttcattaatt
    13741 gacagaccaa tcgaacgaaa ctgtaaattt cgataggata atgattggtc aaactcattc
    13801 attttcaaga catctcgatt catattcaag ataggaatct ttttatcaac atcactttat
    13861 tcgtattttc ctctaccata aaagcttcac gctccacaaa attgttttat acactactat
    13921 tcagcccatt caacgaccaa tgaatt
//
LOCUS       DACKAC010000070        13707 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.071, whole genome shotgun sequence.
ACCESSION   DACKAC010000070 DACKAC010000000
VERSION     DACKAC010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.071"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..903)
                     /locus_tag="I0B46_002235"
     CDS             complement(<1..903)
                     /locus_tag="I0B46_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAR2084497.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFSES"
     regulatory      complement(927..971)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1186..2154
                     /locus_tag="I0B46_002236"
     CDS             1186..2154
                     /locus_tag="I0B46_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAR2084498.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            2267..2545
                     /locus_tag="I0B46_002237"
     CDS             2267..2545
                     /locus_tag="I0B46_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAR2084499.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            2663..2899
                     /gene="secG"
                     /locus_tag="I0B46_002238"
     CDS             2663..2899
                     /gene="secG"
                     /locus_tag="I0B46_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAR2084500.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3011..3757
                     /locus_tag="I0B46_002239"
     CDS             3011..3757
                     /locus_tag="I0B46_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAR2084501.1"
                     /translation="MRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSLAEQ
                     QLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            3760..6117
                     /gene="rnr"
                     /locus_tag="I0B46_002240"
     CDS             3760..6117
                     /gene="rnr"
                     /locus_tag="I0B46_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAR2084502.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQIEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKFGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            6139..6603
                     /gene="smpB"
                     /locus_tag="I0B46_002241"
     CDS             6139..6603
                     /gene="smpB"
                     /locus_tag="I0B46_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAR2084503.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            6905..7624
                     /gene="atpB"
                     /locus_tag="I0B46_002242"
     CDS             6905..7624
                     /gene="atpB"
                     /locus_tag="I0B46_002242"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAR2084504.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            7677..7892
                     /gene="atpE"
                     /locus_tag="I0B46_002243"
     CDS             7677..7892
                     /gene="atpE"
                     /locus_tag="I0B46_002243"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAR2084505.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            7983..8507
                     /gene="atpF"
                     /locus_tag="I0B46_002244"
     CDS             7983..8507
                     /gene="atpF"
                     /locus_tag="I0B46_002244"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAR2084506.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            8494..9036
                     /locus_tag="I0B46_002245"
     CDS             8494..9036
                     /locus_tag="I0B46_002245"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAR2084507.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9059..10615
                     /locus_tag="I0B46_002246"
     CDS             9059..10615
                     /locus_tag="I0B46_002246"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAR2084508.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            10629..11531
                     /locus_tag="I0B46_002247"
     CDS             10629..11531
                     /locus_tag="I0B46_002247"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAR2084509.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            11554..12960
                     /gene="atpD"
                     /locus_tag="I0B46_002248"
     CDS             11554..12960
                     /gene="atpD"
                     /locus_tag="I0B46_002248"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAR2084510.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            12974..13396
                     /locus_tag="I0B46_002249"
     CDS             12974..13396
                     /locus_tag="I0B46_002249"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAR2084511.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4532 a         2452 c         2987 g         3736 t
ORIGIN      
        1 actttcggaa aaaagacgag tatctacttt tgtcataatt ttactgttag caggtttctg
       61 ccaaaattca taacgatcaa ccaatgtttg tccacgactt attccctgag tttccacagc
      121 ggtgaagctt ttgaaacctt cacagatggc cccctcgatg aaataagcaa cagctaacgg
      181 atcattgatg acacatccta aaattctttc ttgtttccag tggaagtcaa aataaaaacg
      241 agtgatcgct tttaaatact ctccctcttc aggattcatt tgacagcagt attcgaggat
      301 agtcggtgtc aagacgattt ctcttgtcac atctaaccca accatttcta tcgtttgttt
      361 gagattctcg aatacataag aagctgcttc aggatcacac cagtaattgt attcagcaac
      421 tggcgaacag ttaccatggc ttttgtaagt tccacccatc gagacaaaac gatccatatg
      481 tttcccaata tttgggttga ctttcaaagc aaaagcgata ttggtcaaag gacccaaagc
      541 aatgactgat gtatttgtct tttctttgaa ataatcagcc aaaaaatcaa cggcatggag
      601 aggttctgcc tgttttctca agatcatcgg gaaatttgtt tcccctaaac catccatacc
      661 atgagtgtct tgtgcactga tgaaaggttg cttcaaaggt ttttccattc cttgataaac
      721 aggaatgtct aaacgatcac agcgttccag gcatttcagc acattctgcg tacctatatc
      781 tgcagggaca ttcccacaaa ctacggtaat agcaactact tcgatatcag gggatttgac
      841 agctaatgat aaagctaaag tgtcgtcgat ccctggatcg caatcaataa ttacttttcg
      901 cattatttcc aatctccttt ctttagagat acttagtttt ttattctggg aagtttccga
      961 accagtccaa gacgagcgtc ttgattataa tgcacgaaat tagtatactg gcttttattg
     1021 cgaatgtaaa tgctctctta caagtaaatc tattgaggag aactttaagt aaatgataca
     1081 atgaaaaaga aatggaattt acgtgtgccg aaaataaaga atcctatgga tcggtacatt
     1141 ggataataga tagattatag tttggcagaa agcggcgggt gtacaatggt tattttttat
     1201 ttcattctta ttctgataat tatagggatc agttaccatt ttctttctta tttttatgca
     1261 aagaaattag taagagcggg agtacagaga ggatatgact ggtatgaaga cactggtcat
     1321 cgtttgatgg accctgctgc agtcacgtca gtagaaaaaa aacgacaaaa attagaagaa
     1381 gagtgtgaaa gcttttggga gcagggaaca cccaaaacaa tcaaaagtta tgacgggttg
     1441 aaactagctg gtcagatgtt catccatcca aataagcaga ataaatgggt catttgcgta
     1501 catgattacc gaagcacagg gaaaagagac atgtctcata taggtaaaag atatgcagag
     1561 aaaggattca acgttcttgt acctgattta cgtgcgcacg gagaaagtga aggagaaatc
     1621 atcggaatgg gatggctaga tcggctagat ttaattgcct ggatccaact catcttagat
     1681 gaacagccga atgcgtccat tattttacac ggcggatcaa tgggagcttc tacgatcatg
     1741 atggcaagtg gtgaaaaact tccatcagcg gtcaaaggat ttattttaga cagcggatat
     1801 gtttctgttt atgcagaatt ccgatatatg ttaagtaaaa tcactgtctt tcctaaaaaa
     1861 atggtcatga gatatgcgaa tcattatgcc cagaaatacg ccggttactc cttaaagcaa
     1921 gcatcagcta cgagacagct tggcagcaac catctgcctt tattagtcat ccatggtgaa
     1981 agagatcatt ttgtccctac agaagcagcg tacacgattc aaaatgctac ggcaggggat
     2041 aaagcactgc tgcttgttcc agaagcagaa cacttggaag catcgatgaa agatccgaaa
     2101 acctattgga cagtgatttt ttcatttatt gaacaaagaa taacgatgga ttaattttct
     2161 cttctttttt gtaagtaatt cgaggataaa taattgttta tagatctgga gactgctaaa
     2221 ataaggtagg aataaaaaaa tatcagttat ttgaggagtg cggcatatgg aaatcaaaga
     2281 agaaaaaaat cgtcttgctt tattcaatga tgagcaacaa gagattggag aaatgacatg
     2341 gtcagatgcg ggcccagaca tcatgatcat tgaccataca tttgtagatc cagcatatcg
     2401 tggtcaaaag ttagctgaaa agctagtgta tactggagta gaattagcaa gacgagacgg
     2461 gaaaaagatc attcctttat gcccttatgc aaaaaaagag tttgaaaaga aaccagaata
     2521 tcaagacgtc ttacgtcagt cataagaata acagctgaaa gttttgaact ttcagctgtt
     2581 attccattta tatagagttg tcacggactt ttatctatga taaagttaaa gagacgatat
     2641 cttttgggaa ggaggctgcg atatgtataa tttaattttg ggaatcgtga tcgttttatc
     2701 gattgttatg gtcattgcga ttatgatgca gccaagcaag caaaatagtg cagcgagtgc
     2761 atttaccggc ggtgcggatc aactattcgg caaacaaaaa gctcgcggct ttgaagctgt
     2821 catgcagcga tcaactgcag ttatgggagc tatctggatg atcttattat ttgtcctagc
     2881 atatttatct tcgaagtagt tgaataagcc tctcgcaaaa cggcgggcgg ctttttttat
     2941 tgcaaagaga acgcttaaga agaagttatg aaagaaacta tggtaaaatg aggcgaaaga
     3001 ggtgagacga gtgagacaat taccaaaacc cctttatgca aaacatggaa agcgagcggt
     3061 tttgctgctt cacgcttact caggaagtcc aaacgatgtt cgaatgctcg cacgattttt
     3121 ggaaaaagca gattatacag tttatgcgcc gctttttaaa ggacacggaa caatggagcc
     3181 atatgatatc ttgcaagaaa aagcagaaag ctggtgggca gacactaaaa aagccatcca
     3241 ttttttacag tctgagcagt tttctgatat tgccgtatta ggattatcaa tgggcggcat
     3301 ctttgctgta cgagcactag aagaagaatc agtgattggc ggcggttttt tctgttcgcc
     3361 tttgtctcct gtaaaaacaa acgtgcctga aaactttgaa aaatacgtac gtcaagtgct
     3421 gaagacagca ggtaaatcag agaaagaaat caatgaaaaa gcagttgctt accgctcgtt
     3481 agctgaacaa caattaatgg atatacaaga tcaggcagca atcgtagaaa gtaggttgtc
     3541 agatatccaa cagccgatat ttttagctca agctgggaaa gatgaaatga tcgaccctaa
     3601 tggcgtattt gaaactgcac gcaagttgag ccgtcagcga gtgacacttc aatggtatcc
     3661 tgaaagtggt catgtgatta cagttggtac agcaagacgc gaattagaaa aagacgtctt
     3721 ggaattttta gaaaaattac cttggaatga ggaataaata tgacaaaaga aacgataaaa
     3781 gagaaaattc tctttttcat ggaaagcagt cgcaaacgca gtttttctat ggaagaaatc
     3841 gctgaaggtt tagggttcca aaaaagcgat gattttaaat tactagtaca aacgattgct
     3901 caaatagaaa gagaacagag tatagttttc aataaaaaag gaaaagtgaa acttccttta
     3961 aaaccaatct tgatcgaagg aacatttcga gcaaatgaac gaggatttgg atttgtaacg
     4021 attgatcccg aagaagatga catctatatc ccaaaagaag cgactggttt tgcaatggat
     4081 ggagacaccg tagcaatcga tatcatgaag cgagcaaata cagatatgga tcgcggagca
     4141 gaggggaaag tagtcgagat tcgagaccgg gcgactactc agttagcagg ggaattcgtt
     4201 gcttattcag aagaagaaat acaagaaaca gatctttacg gcgttgttat cccgaaagat
     4261 agaaagctca accaatttaa agtatatgca gcagcagagg gagttcgtcc agttgacgga
     4321 agtattgtgc tgatcgaact gacccattac cctgaaaaag ggtatgcgac tagtttagaa
     4381 gggataatca agcaagtgat tggtcataag aatgaccctg gcatggatat cctctcaatt
     4441 gtcgtggcta atggtatccc tacgaaattt cctgatgatg tattgtctga agcagatgaa
     4501 gttccagatc aaatcagcga aaaggatata actggacgta gagatttaag aaatcagctc
     4561 attgttacga ttgatggaga agatgcaaaa gatctagatg atgcagttac ggttaaaaaa
     4621 ttagataacg gtaattattt tcttggagta cacattgcag atgtttctta ctatgtgaca
     4681 caaggcagtc aattggacag agaagcatat gaaagaggga caagtgtata tttgactgat
     4741 cgagtgattc cgatgatccc gcaacgttta tcgaatggta tctgttcact gaatccccat
     4801 gttccaaggc tgaccatgag ttgcgaaatg gagattgatc caaatggaca agtagtctct
     4861 catgatattt tcccaagtgt gatccaaaca acagaacgga tgacctacac agctgtcaat
     4921 caaatcttag aagatcaaga cgaagaaacg atgcaacgct atgctcagct cgtacctatg
     4981 ttccaagaaa tgggtgaatt gcatcgaatc cttgaaacga tgcgaatcaa acgtggcgcg
     5041 atttcttttg aggatcgcga agcgaaaatc cttgttgatg ctgaaggaca cccaaatgac
     5101 atccagctaa gaagcagagg aattggtgaa cgcttgatcg aatctttcat gcttgcggcc
     5161 aatgaaacgg tagctgaaca ttataataaa ttaaatctgc cgtttattta ccgaatccat
     5221 gaacagccaa aagaagaaaa aatgcagcgc ttctttgatt ttgcttcagc tttgggcatt
     5281 ctggtccgag gaacgaaagg aacgatcaca ccaaaagatt tacagcatgt gatcgaagga
     5341 gtagaagaca aaccagaagc tgcagttatc aatactatgt tgctaagaag catgcaacaa
     5401 gcacgctatt cagaagacaa ttttgggcat tatggcttag cggcagatta ctacacacac
     5461 tttacatctc ctattcgacg ctatcccgat ctaatcgtcc atcgtctgat ccgttcttac
     5521 agtgaagata aaagtgaaag caatcaaagt tcgtgggctg cggaacttcc tgaaattgca
     5581 gatcatagtt ctaagatgga acgtcgtgca gtagaaacag aacgtgaagt agattccatg
     5641 aagaaagcag aattcatgat ggacaaagtg ggagaagaat tcgatgggat catcagctct
     5701 gttgtgaaat tcggcctgtt tatcgaactg ccgaatacag tagaaggatt gatccatatc
     5761 aatgaattga aacaggacta cttccatttt gttgaaaatc atttagctct tgtaggtgaa
     5821 agaactggtc tgacttttaa aatcggacaa aaagtgcgcg taaaagtagt caaagctgat
     5881 ccagatgaac gagcagtgga ttttgaactc gtttccgcag aagaggtcac tgcgttggaa
     5941 cgtccaaaag cacgcaatgg aaaatttgga aaacgagaag gaaaacaagc aggcaaacgc
     6001 tcagaaaaaa atggcaagaa gaacaaacga tatagtgata gtaagcaaac gttcggtaag
     6061 aaaaaaggga aaaagccatt ttacaaagat gtcaaaaaga agaaaaagaa aaaataaaat
     6121 gagttggagg aaacagctat gccaaaaggc gagggaaaac taattgcaca aaacaagaaa
     6181 gctcgccatg attattcgat catcgacacg atggaagcag ggatggtctt gcaaggaacg
     6241 gagatcaagt cgatacgaaa cagccggatc aatctgaaag atggatttat tcgcgtccgc
     6301 aacggggaag ctttcttgca taatgttcat atcagtcctt atgaacaagg aaatattttt
     6361 aatcatgatc cgttgcgcac gagaaagtta ttattacaca agaaacaaat cgttcgtctt
     6421 gaaaacgaat tgaaaaacac tggaatcact gttgttcctt taaaagtcta tattcgtaac
     6481 ggctatgcca aggtattgat tggtctggca aaagggaaaa aatcgtatga taaacgggaa
     6541 gatttgaaac gaaaagatat cgatcgacaa attgatcgaa cattaaaaaa tttctctaga
     6601 taatttcatt atcctttttc gcatattcgg tgcttttgct tttacttaat gataaaattt
     6661 gttagcattt aatctgataa aacatgatta tcacaggttt tttcttagat caggagcacc
     6721 gtgcatgggg aaaaattcgc ttcgtttttg actgaaataa aaaagtgtct aattttcatg
     6781 aaaatgtttt gaattttctg tttcatggtg gtatgatacc gtgtggttag aaaccgattg
     6841 gaaaaaacca gatacgcaca tgaattgatt ttatgtgaat ttcggggaaa agaggtgaaa
     6901 tggattggat gaacgctcgc taacgtttca cattggacca atttggtttg atggtacagt
     6961 ttgtataatg gtgttactga cctgtttgat tgttttcttc ttagcttact tttttacaag
     7021 aaatcttaag ttgaaaccaa caggcaaaca aaatgcgata gaatgggtca ttgattttag
     7081 tcgaggaatc gtaacagata atctgcctag aaaagaattg agcaatttcc atttgttggc
     7141 atttacattg tttttatttg tttttgttgc aaacaacata ggtttagtaa caaaaatcgt
     7201 cttacctaac gaaacaacac tttggaaaag cccgacagca gacccgtttg ttactttaac
     7261 tttggcattt ataatgatct tacttactca cttattcggg gtgaaaaagt taggcttcaa
     7321 aggatatttc gtaaattcgt tcttgaaacc ctatagtttt atgtttccga tgaaactgat
     7381 tgaagaattt acaaatttat taacacttgc tttacgtctt tatgggaata tttatgcggg
     7441 ggaagttttg ctaaccttga ttgcaaaaat gatgacaagc ttaggctggt tctcattacc
     7501 attagcaatt ccattagaga tggtctggat cgcgttctcc ttgtttattg ggagcatcca
     7561 agcatttgtc tttgtcactt tgtcaatggt ttatatgagc cataagattg aagtagaaga
     7621 ataatactcg gttaaaaaac aaactactat ttatctattt taggaggaaa acaattatga
     7681 attatatcgc agcagcaatc gcaatcatgg gagcagcaat cggagccggt tatggtaacg
     7741 gtcaagttat ctctaaaaca atcgaatcaa tggctcgcca acctgaaatg tctggtcaat
     7801 tccgtacaac aatgtttatc ggggtagctc tagttgaagc tgttcctatc ttaggggtag
     7861 ttatcgcctt gattctagtt tttgccgttt aattgaatca gtattaaggc agaaacaaca
     7921 tcgtttgtta ctgctttttt acatggaaac tagaaatact ccaaagaaag gaaggctcgc
     7981 ctatgctgaa tcaattggca attgcagaag ttggcaatgc gatgttaggt aatatcatcg
     8041 ttgtcagcgg ctcatttttg atactactgg ctctcttgaa gcactttgct tggggaccaa
     8101 tcagcgatat tttgaaaaaa cgtgaagaca agatcgccaa tgatttagat tctgcagaac
     8161 aatctcgcat caactcagcg aaaatggaac aagaacgcga acaacaattg ttagcctctc
     8221 gttctgatgc agctgatatc atcaaaaatg cgaaagaaag tggagaatta agccgccaaa
     8281 atattttgaa ggatgctcaa gaagaagcag ctcgtctaaa aagcaaagcc caagctgata
     8341 tcactgtaga acgtgattca gcgctgaact ctgtaaaaga cgacgttgca gaactctctc
     8401 ttcaaatcgc ggaaaaaatc ttgaacaaag aattatctcc agaaatgcat gaatcattaa
     8461 tcaatcaata cattgaaggt ctaggttctt caaatgaaac tagataaata tacagtagga
     8521 agacgttacg ggaaagcttt atttgaactg gcgatcgatt caaatcaagc agaagaaatc
     8581 tatcaagatc tcgtaagttt acgtcaaatc tatgaacaag tacctggatt agggaatatg
     8641 ttaagtgatg ttcgtctaga acctcatgaa aaacgagaaa tcatggacaa gcttgtcagc
     8701 ggttatgatg ggatcgttaa aaacttttta gaagttgttt atgagtataa ccgaatggat
     8761 gaccttttgt tcatgatcga agaatatgaa cgtcgatatg atgaacatca agggttacta
     8821 ttgggaagtg tcacaacagc agtcccactt tcagacgaac aacgaataca attagaaaag
     8881 aatgttgcaa aaacaatgga ctatcaatca gttgaattaa aacaaatcgt tgattcttct
     8941 attattggtg gcgcgattgt tgaagccaac catcgagtaa tcgacggaag cattcgcacg
     9001 caattagaaa aaatgcgtaa tcaattaaac agatagtaca aagaggtagg tgaatcgaat
     9061 ggccatcaaa gcagaagaaa ttagtgcctt gattaaagaa caaattgaaa attatcaaaa
     9121 cgtgctttca gtcgaagaaa tcggtaccgt aacttatgta ggtgacggga tcgctcgtgc
     9181 acatggttta gaaaacgcga tgagtggtga gttgcttgaa ttttcaaacg gctcatacgg
     9241 aatggcgcaa aacttagagt caaatgatgt aggtatcatt atccttggcg atttcgagtc
     9301 cattcgtgaa ggagataaag taaaacgaac aggtaagatc atggaagttc cagttggaga
     9361 ggccttgatc ggtcgggtag tcaatccgct aggtcaacca atcgacggac taggtgaaat
     9421 cgttactgac aaagctcgtc cagtagaagc gatggcacca ggtgttatgc aacgtaaatc
     9481 tgttaacgaa ccaatgcaaa cagggctaaa agcgatcgac gcccttgtgc caatcggacg
     9541 cggacaacgt gaattagtca tcggtgaccg taaaacaggg aaaacttcta ttgcgatcga
     9601 tacgatcatc aaccaaaaag gtcaagatat gatctgtatc tatgtagcaa tcggacaaaa
     9661 agattctaca gttcgtacac aagttgaaac attgaaaaaa tatggcgcaa tggattacac
     9721 aatcgttgtg aatgccggtg cgtctcaacc agcaccattg ctttatatcg caccatatgc
     9781 tggtactgca atgggtgaag aattcatgta caacggtaaa catgtattga tcatttttga
     9841 tgatctttca aaacaagctg ttgcttatcg tgaactttcc ttgctgcttc gccgtccacc
     9901 aggtcgtgaa gcttatccag gggatgtttt ctacttacac tcacgcttat tggaacgtgc
     9961 agcaaaatta agtgatgatt taggtggcgg ttcaatgact gccttaccat ttgttgaaac
    10021 acaagctgga gatatctctg cttatatccc aacaaacgtt atttcgatca ctgatggaca
    10081 aatcttctta gaaagcgact tgttctacgc aggtactcgt ccagcggttg atgccggact
    10141 atctgtatct cgtgtagggg gttctgcgca aatcaaagca atgaaaaaag ttgctgggac
    10201 tttacgtcta gacttagcaa gttaccgtga attagaagca tttacacaat tcggttctga
    10261 cttggatgca gcaacacaag caaaactaaa tcgtggacgc cgtacagtag aaatcttgaa
    10321 acaaaaattg catgcaccgc tagctgttga aaaacaagta gtgattttgt atgctttgac
    10381 acatggattc ttagatagta tccctgtaga cagtatttta gacttcgaac atgaattatt
    10441 cgaatatctg gataccaatc attcagatat ctttgaaaca atccgtacaa caaaagatct
    10501 tccagaagaa gaagcgttga acagtgcgat tcaggaatat aaagacatgt tcttagctac
    10561 aaaaggaaat aattcctcta cagaagataa attgaaatcc attcaaaatg catagtgagg
    10621 tgagatgaat gggtgcttca ttaaatgaaa tcaagacacg tatcgcttca acgaaaaaaa
    10681 cgagccaaat cacgcgggca atgcagatgg tgtctgcttc taagttgaca aaatctgaag
    10741 cttcttcaca gaagtttcag gtttatgcaa caaaagtaag agaaatcgtt acgcatttga
    10801 ctgctactca gttaaatgat attgcctcag aaaatcctag aggggatatc aattacaaca
    10861 gcatgttgct cagccgtcct gttaagaaaa cgggttatat cgtcatcact gcagatggcg
    10921 gtttagtcgg tggatataac agctcgatat tgaaacaaac gatgtctatt ttagaagaag
    10981 accaccaatc acccgatgat tacgtgatga tcgcaattgg cggaactggt gcagacttct
    11041 ttaaagcacg cggtatcaat ctagcatatg agctgcgcaa tctatctgat cagccaagct
    11101 ttgatgaagt ccgcaaaatc gtaaatatgg caacaactat gtaccaaaac gaagtattcg
    11161 atgaattata cgtatgttat aaccatcata tcaattcgct aactagtcag tttcgtgtag
    11221 aaaaaatgct gccaatctca gacttagatc cagaagaagc aactactttc gaacaagaat
    11281 atatttttga accttcaaaa gaagaaatct tgtctcagtt attgcctcag tatgctgaaa
    11341 gtttgatcta cggtgcgatc gttgacgcca aaacagcaga acatgctgct ggtatgacag
    11401 caatgaaaac agcaactgat aatgctgcta atatcattga tgagctgacg gtttcttaca
    11461 accgtgcaag acaaggggct attacccaag aaattaccga aattgttgcc ggagcttcgg
    11521 cactagaata gtattgagat tggaggaaaa aagatgagtt caggcaagat tgttcaagta
    11581 atcggtcccg ttgtcgacgt ggaattttct ttagaccaat ccttaccaga tatcaacaac
    11641 gcattagttg tttataaaaa tgacgaaaat aaatcaaaag ttgttcttga agcagcgtta
    11701 gaattagggg acggagtgat ccggaccatt gcgatggaat caacagatgg tttacaaaga
    11761 ggaatggaag tcattgatac aggcaaagca atctctgttc ctgtaggtaa agaaacatta
    11821 ggtcgcgtat tcaacgtact aggagatacg atcgatttag aaacaccttt cccagaagat
    11881 gcggaaagaa gcgaaattca taaaaaagca ccagcctttg acgaattaag tacaagtaca
    11941 gaaattttgg aaacagggat caaagttatc gatttgcttg ccccatattt aaaaggtggg
    12001 aaagtcggac tattcggtgg tgccggtgtt ggtaaaaccg tactgatcca agaactgatc
    12061 cataatatcg cccaagaaca tggtggtatt tctgtattta ccggtgtagg tgaacgtact
    12121 cgtgaaggta atgacttgta ttatgaaatg aaagattcag gagttatcga aaaaacagcc
    12181 atggtgttcg gacaaatgaa cgaaccacca ggtgcacgta tgcgtgttgc tttgactggt
    12241 ttgacgattg cggaatattt ccgtgatgta gaaggtcaag atgtactgtt gtttatcgac
    12301 aacattttcc gtttcactca agctggatct gaagtatcag ccttgttagg acggatgcct
    12361 tctgcggttg gttatcaacc aacattggca acagaaatgg gtcaattgca agaacgtatc
    12421 acatctacga aaaaaggttc gatcacatcg atccaagcaa tctatgtgcc agccgatgac
    12481 tacactgacc cggcgccggc aacagcattc gcccatttgg atgcgacaac caacttggaa
    12541 cgtaaattaa cagaacaagg gatttatcca gcggtagatc cattggcttc ttcttcaagt
    12601 gctttagcac ctgaaatcgt gggagaagaa cattataaag tagcaacaga agtccaacac
    12661 gtattgcaac gatatcgtga attacaagat atcattgcta tcttaggtat ggatgaatta
    12721 tccgatcaag aaaaagtgtt agtttctcgt gcccgcagag tccaattctt cttgtcacaa
    12781 aactttaacg tggctgaaca atttactggt cttccaggtt catatgtccc tgtagaagaa
    12841 acagtgaaag gattccgcga aatcttggaa ggcaaatacg atgatcttcc tgaagaagct
    12901 ttccgaagtg ttggccgaat cgaagacgtc gtagaaaaag cgaaaacact aggctactag
    12961 aaaggggtgc cccatggatc aatatttaac agtaaatgtg gtaactcctg ccggtttggt
    13021 ctatgatcac catgcagcta tcgtcgttgc acgtacgaca gctggtgagc ttgggatttt
    13081 gcctaaacat gcaccaatca tcgttccatt gacgattgat gaagttcgtg taaaacgaac
    13141 agattcagat acccacgtcg attggatcgc tgttaatggc ggaatcatgg aagttcgtga
    13201 taatgttgtt tcaatcgttg ctgatagtgc ggaacgtgaa cgtgatatcg atgtatctcg
    13261 tgcggaacgt gcaaaacaac gcgcagaacg ccaaatcgaa gaagcaaaag caaaacaaga
    13321 tacgaacgaa ttgaaacggg caactgttgc gttgcaccga gcaatcaatc gtatcaaagt
    13381 atctaagcac tcataaaatg agatgcggat gtttatccgt gtttaagaac agtaaaaaga
    13441 ggttatgaca gaagtggtca gcttcaagac ataagaagga atttacgaaa attgcttctc
    13501 aaatttttgt gaaatctcag cttatttttc gaagaagcta cttctggaac accgtatatc
    13561 ggaggttgtg aaatcagcgt tttgtcctga gtattagaga aacaaatgga aaatagttcc
    13621 tcatattttg ccgttttttg agctaatacc gagggtcaga tgattgaaca ccgtttattt
    13681 ggaataaggg actgcgacaa gactttt
//
LOCUS       DACKAC010000071        13536 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.072, whole genome shotgun sequence.
ACCESSION   DACKAC010000071 DACKAC010000000
VERSION     DACKAC010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.072"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(60..1007)
                     /locus_tag="I0B46_002250"
     CDS             complement(60..1007)
                     /locus_tag="I0B46_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAR2084512.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            1197..1466
                     /gene="rpsN"
                     /locus_tag="I0B46_002251"
     CDS             1197..1466
                     /gene="rpsN"
                     /locus_tag="I0B46_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAR2084513.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(1642..2004)
                     /locus_tag="I0B46_002252"
     CDS             complement(1642..2004)
                     /locus_tag="I0B46_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAR2084514.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(2015..3136)
                     /locus_tag="I0B46_002253"
     CDS             complement(2015..3136)
                     /locus_tag="I0B46_002253"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAR2084515.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYIHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(3152..3502)
                     /locus_tag="I0B46_002254"
     CDS             complement(3152..3502)
                     /locus_tag="I0B46_002254"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAR2084516.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGKVWVSIT
                     HTNETAFAQIILED"
     gene            complement(3632..5143)
                     /locus_tag="I0B46_002255"
     CDS             complement(3632..5143)
                     /locus_tag="I0B46_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAR2084517.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQSETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(5465..6817)
                     /locus_tag="I0B46_002256"
     CDS             complement(5465..6817)
                     /locus_tag="I0B46_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAR2084518.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKDFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVPDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAVKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(6970..8046)
                     /locus_tag="I0B46_002257"
     CDS             complement(6970..8046)
                     /locus_tag="I0B46_002257"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAR2084519.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            8242..9987
                     /locus_tag="I0B46_002258"
     CDS             8242..9987
                     /locus_tag="I0B46_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAR2084520.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDSVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLKKEEQTQASRKTEIVHEKEFGSFAFRASTSTEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(10236..10703)
                     /locus_tag="I0B46_002259"
     CDS             complement(10236..10703)
                     /locus_tag="I0B46_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAR2084521.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFCQALKKAIDQGLPSTEDSR"
     gene            complement(10687..12060)
                     /locus_tag="I0B46_002260"
     CDS             complement(10687..12060)
                     /locus_tag="I0B46_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084522.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGIMYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDAAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(12381..12869)
                     /locus_tag="I0B46_002261"
     CDS             complement(12381..12869)
                     /locus_tag="I0B46_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAR2084523.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(12891..13493)
                     /locus_tag="I0B46_002262"
     CDS             complement(12891..13493)
                     /locus_tag="I0B46_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAR2084524.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         3863 a         2938 c         2458 g         4277 t
ORIGIN      
        1 ttttgtcttt gcccctacaa aaatgtttca gcttttttcg ttttttattc ctgtatttct
       61 tatttttcga atgacaggat gattttccca gcaggaggat gtccttcaac tagctgatgt
      121 gcttggatga catgcttaag ggaagcagac agattcattg aaataaaaac atgaaaggct
      181 cctttttggt aggctttgat tatgccagca aaggcttctg catctagata ttcctttctt
      241 ggaacaaacg attcataaaa gccttccttt ttttgccgca aatctagatc tgggaggtca
      301 tttaatgcaa catacctgcc gcctggtttc atgatctgga tgccggtttc tcctttgata
      361 cttcctttcg tcgcatcgat tacaaggtct gcctgatcag caaattgcag acctggattt
      421 gttttatcat aagcagcaaa tgctgaagct cctaattttt ttaccttttc ctcattttta
      481 ctggatgcac tggttaagat tcggattccc ttttcatgga gaagctggat cagacttgac
      541 ccgaccgcac ccgatgctcc tagtatcatt acagtatctg tcggctggat ttccgtcaaa
      601 tggttgatta gattataagc cgtaattcct ggcgtgacca ttccggctgc ttcttcccaa
      661 ctcattttat ctggaatttt tgctactttt gctgatggca acacgacttc ttctccgtaa
      721 gtcccgccga ctgcatggac agctactcga tcacctacat gaacagttgt gacatctgaa
      781 ccaatctctg tcacaatacc agcgccatca ttacctggta cataagggaa tttcaatgtt
      841 cgtatctctt tcatttctcc taaacgcaag gcaacatcat aaggattcac actgaatgct
      901 tttatttcta cacgaacatg tccagaaccg acttctctgg gatgagcatc gatttcttca
      961 aaaacatctt gtgcttctcc gtaaccgtta attgcaaatc ttcgcatggt caccatctcc
     1021 ctttttcttt aagtttacgt ttatgccttt attttgacaa aaaaaatgtc ttttcgtttt
     1081 ggcgcatgat tcaaaacaaa atagttgtct tttttcgcgt aacgtgttaa attatttatc
     1141 taaaagcgta aagattacga tttacgctaa taaaaaataa ttggaggaat ttgttgatgg
     1201 ctaaaaaatc aaagattgcc aaagcaaaaa aacagcaaga aacgattgaa cgctatgctg
     1261 ctttacgcca ggaattaaaa gcgcaaaaag attacgaagg cttgcgcaaa cttcctttag
     1321 atgctcgtcc agaacgattg aaaaatcgtg atttgatcga tggacgccct cgcggataca
     1381 tgcgtaaatt cgggatgtcc cgtataaaat tccgtgaatt agcacataaa ggtttgattc
     1441 caggagtaaa aaaagccagc tggtaatcca agtctggctg taatgtaaag gaggagggcg
     1501 catggaccac tccttggatc atcaaaaaag atacgttcgc ttcttctagc agaaatgctg
     1561 gtactagaag cagacgtatc tttttttacg cacagctggt ccttttttat atgtaaaggc
     1621 tgcatggtta cttcgctata tttatttagt aacaggagtc agtgcttcga tacccacact
     1681 aattttcaat gctcggtcaa cttctgccat aatgggtcga tccaaatggc agaccttttc
     1741 tttcaatctt gatttatcga tcgttctgat ctgctccaac aggataacgg agtctctttc
     1801 tattcctgtt ccagtagctt tgatcccaat atgagtcgga agcttaggtt tagccatttt
     1861 tgccgttatg gccgcaacaa tgatcgtcgg gctaaaatgg ttacctagat tattttgaat
     1921 aaccaaaact gggcgtgttc cgccttgctc cgatcccacg actggagaaa gatcagcaaa
     1981 ataaatatct cctcttttta ccataggatc ctccttattc ttgatattct cttggtatcc
     2041 gctgtcctaa tccgcaagcc acttcatagt ggatcgtacc taattgatct gctatatcct
     2101 gcatagtgat ttctttgtct gcatttttgc cgataagcgt cacttttgtt ccgacaggaa
     2161 attcttgagg caatcggatc atcagttgat ccatacatac acgcccaatc gtttcacagt
     2221 agttcccttc taccaataga gaaaaaccct gcatctttcg tggccagcca tctgcataac
     2281 cgatcggtat cgttccgatc cactctgctt caggagtgat atatgtttct ccatagccga
     2341 ttccttcacc tgctgggagt tttttgactt gaattaattc agaaaccagc tcaagtgctg
     2401 gctgcagtgg ataaacttcc ggtagtgcgt gcccagaggg attcagtccg tacattgcta
     2461 ctccataacg aatcatattg ccgattgttt catcatgcca taaagccgtt gcactattgc
     2521 tgacatgtat atatcgagga agttcgggaa gtttttttag cacttctata aagcgttctt
     2581 tttgcagatt ccaatatgta tcatctgcct gatctgctgt agaaaaatgg gtaaataaac
     2641 cttcccattc taatacacga ctttctttga tgaatgcagc tgcctctttt acttcttcag
     2701 gtgtacaaaa tccgatacga cccatcccag tgtctgcttt tagatgaatc gaaagaggcg
     2761 tctctagctt acttttctct aaaacagcag ctgctgcctg caaccattcc cgtgtacctg
     2821 cagttactga taaatcatga gcaacagcta cagtcagtgc gtcgtaagga accatattca
     2881 agataaggat gggctgagta aatccagctt ctctcaactc gactccttca tctaaatttg
     2941 aaacacaaaa gcccgaagct ccacctgcaa ctgctgcctc agctgtttca atagctccat
     3001 gaccgtatcc gtttgctttt actactgcaa acaattcttt tccttgcgga agtcttgcaa
     3061 cttcatttgc tacattttca gttatagctt ttttatgtat cactgctttc gttgggcggt
     3121 gccaagcgac aaccatcgtt tttccttctt tctagtcttc caaaataatt tgtgcaaaag
     3181 ctgtttcatt tgtatgagta atactgaccc atactttgcc ttcatgaggt gaacgcgtca
     3241 caacaggctg accgttttca ttttttaaaa tctcgacatc ttgaaaggta acttttccga
     3301 tcccagttcc ccaagcttta gagaaagcct ctttgcaagc gtaacgcccg cctaaaaatt
     3361 ctacttggcg atgaaaaggc aaggactgga atagtttcat ttcatcagag gtcaagattc
     3421 gtgcaatgaa ttttggtttt tcttcgatca gccgagtgat cctaggaagt tcgactgcat
     3481 cgatcccaat tccttttatc atatgctttt ctcttttcaa taaatttaga acactctcat
     3541 tctaccaaat tataaccatt ttgccacaaa taagtccaca aataacaaaa atgaaagttt
     3601 ctcttaaaaa agagaaactt tcattttatg tttaatcgtt atttgaacgg attacaaatc
     3661 cgcgtttttt atcgttgtga cgtttaccgc catctttttg gctgttatta tttttgctat
     3721 aaccgccttt tgatttgttt ccacgataat taccgccgtc acgacgatta cggttgtttc
     3781 cgccaccacg tttgccattt ttattgtatc cttttttctg cattggcaat gggcgttctg
     3841 gtgtgatctt cactggcact gcatcagctg gatcttttgc tgtcgttttt agcaaaagtg
     3901 caactaaatc ttgtgcagaa tattcttcta ataatttatc tgctgtttgc aggtacttat
     3961 ctagaccatt ttctgcaagt tttgtttcga tttgttcaac tgcagcacct aattgacctt
     4021 tgaatgcttc tttttcggtt ggtggacgta aagtcgtcat ccgttttttc gtcaagtttt
     4081 caatcacatg caagtagtcc atttcatttg gtgtaacgaa agtcacagac ataccgcctt
     4141 ttccggcacg gcctgtacgt ccgatacggt gaacgtagct ttctggatct tgagggatat
     4201 catagttgta cacatgagtg acacctgaaa tatctaatcc acgtgcagca acgtctgtcg
     4261 ctacaaggat atctaaatga ccgctcttaa atgaacgcaa tacgctcatt cgtttttgtt
     4321 gagacaaatc gccatggatt ccttctgcac ggtaaccgcg tgcttctaat ccgcgtgcca
     4381 attcgtctac acggcgtttt gtacgtccga agacgattgt tagttcaggt gtttgaacat
     4441 caaacaagcg agtcatgata tcgaattttt cgtattcttt tgcgcggaca tagtattgat
     4501 cgatcaagtc agctgtcatt tctttcgctt tgatcttcac atgatgtgga tttttcatga
     4561 atttaacgcc aatattcttg atagctggcg gcatcgttgc tgagaaaagt aatgtttgtc
     4621 tttgatcagg aacttgtgaa atgatttttt cgatatcttc caagaagccc atattcaaca
     4681 tttcatctgc ttcatccaaa actaatgttt gaacggttcc aagtttcaat gtgtggcggt
     4741 tgatgtggtc aagcatacgt cctggtgtac ccacaacgat atgtggacga tctttcaagc
     4801 cacggatctg acggccaata tctgctccgc cgtaaacggc ttgtacacgt acttttttgt
     4861 cgcgccctaa acgatataat tcttcttgtg tttggatagc taactcacgt gttggtgcga
     4921 taactaaacc ttgtaattcg tgacgatctg gatcgatttt ttctaacatt ggtaatccaa
     4981 atgcagctgt tttccctgta cctgtctgtg cttgtccaat aacgtctttt ccagctaatg
     5041 ctagtggaat cgtttctgat tggataggtg ttgcttcttc aaaaccagcg cgttcgactg
     5101 atttcaacaa ttctggtgat aattctagtt ctttaaattt caaatgaatc ctcctatatg
     5161 gttgttaact gaggatgtag cttttttgct accccgtcga tttgttcggt caaagtgtat
     5221 tgtttctttt tgacagtggc cacgcacgtt tttatccatt cttagcccct gtggcaccac
     5281 cgaaaaatca ctttgcgaag tttaggcatt agaaaagcca aacgggtcgc gttcagcttt
     5341 atcattatta tgacatctgg cttcaacaag ttgtttctat ggcaaaacca gtccaatttc
     5401 tcttcatctt tcggcaatag gttattctaa catagttttt cgttttctgc aagtttacag
     5461 atttttattt catttgctga agctgttcta ccacttcaaa taagcccatg ctgttgctgc
     5521 cttttagcat aatactatct gttgcttcaa gattttgttt tacagcatca atcaatttat
     5581 tcttttcttt ttcaaaatag aacacaggta gttgggggta tttttggttc aattcttctt
     5641 ttagtgcatg catttgttcc ccataaagga aaatcatggc gaatttctca ggatcgatat
     5701 gttcactcat ttgcgcatgc atatttaaag actctttccc taattccagc atatctgcta
     5761 acacagcgat tctgcgtcct tttgttggta gatttttaaa agtatctaat accagtccca
     5821 tggctgttgg atttgcatta tagacatcgc tcaaaatagc tgcaccattt cctgccgtta
     5881 gccattcggt tctgttttga gtgatagaaa catgggcgag cccttggcgt atctcaggta
     5941 tggacagtcc aaagaagcgc ccgatcgtat acgcaatcaa agcattcgtt acattataac
     6001 ttccaggcag cgggatcgta aatgtttctc cttctactac aaaatcggtt tgtgcttttc
     6061 cttttgctgt cacttccgca tgaacatcgc catttccaat accgaatgtt gtgactgttt
     6121 gtactaatgt tgccgttaaa ggtgtcagca acggttcatc tgcgggcaca acgagtaatc
     6181 cgtctggtac aagtccgtca gtgatttcca ttttggcttt agcgattcct tctcgcgacc
     6241 caagattttc tatgtgtgct tcaccgatca ttgtgattgc tgcagctttt ggttgagcca
     6301 taagtgaaag ttccgtgatt tcattggcgt gatccatgcc catttccaag acgatctttt
     6361 ctgtatcttg gggcatgtgc aaaatcgtgt aaggaagacc aatattattg ttgtaatttc
     6421 cttgcgtctt atatgttttg tatttttcag acaagacaga cgcggttaga tctttcgtgg
     6481 tggttttacc attgcttcct gtaattgcaa cgacttctgc acctactttt tccaagtaat
     6541 aggttgccaa ttgctgcatg gctttcaaag gatctgctac aggaataact ggaaaatctt
     6601 ttggagcttg ttcaacaggc aagctccaaa atgaagcaga tgctccgttt gtgatcgctt
     6661 gctggataaa ttcatgtcca tctcgcgtac cagcaagagg aacgaataaa cttccttcgg
     6721 ttatcttccg gctgtcaaat tcgacattgg taatctttgt cagtgcagag cattcgctgc
     6781 ccacggcttg tgcaatttct tgtattgtta gattcattga gatctctcct aaaaaaggtt
     6841 gtgaaaaaag cgtttggcct aaacggcggg ttcgcatgac tctcacttcc aagtaaattt
     6901 ctctattcgt atttagaagc tagaatgaac gcggttacgc aatcactcca gcttcttctt
     6961 tatttctctt tattcatttt tttcaaaaaa agattgacgc tgatggtatc gattcattcc
     7021 taattgaatc agttcttcaa tcaaatcacc gtatttcaag cccatatttt cccataagag
     7081 tgggtacata ctgaactccg taaatcctgg catagagttt aattcattca ggaataattc
     7141 gtttttattt gtcaaaaaga aatcgcaccg gctcaatccg cttccaccta acatcgtgta
     7201 agctaacttc gcgtactctt gcgctttttg ataaacttct tccggcactt cggctggaat
     7261 ctgcatttcg attttattat tgatatattt ggcttcataa tcatagaatg ctacgtcttt
     7321 tacgacttcg ccaggcaaag tcgtccgaac atcttcattt cctaatacag caacttcgat
     7381 ttcgcgcgct tcaattcctt gttcaacgat tgctcgagaa tcatactgat aggctgttgc
     7441 taaagcattt tgcagctctt ctcggttttc tgcctttgta atgccgacac tagaacccat
     7501 attcgcaggt ttgacaaaca tcggataaag caaagaacct tcacattgat caaatacttt
     7561 tttaggattt tctttccatt gattcttaag tactggtaca taaggaactt gcggcacacc
     7621 agcagcttgt aaaatatact tggtcatgat tttatccatg gcacatgcac tggtcaatac
     7681 gcctgcgccg acataaggca tattcaatgt ctctaagaag ccttggatcg ttccatcttc
     7741 cccgtttggc ccgtgtaaaa ctggaaaaac gatggctcct tcttctttga tttctcccgg
     7801 attgatcact tttcctgtaa agccttcctc tgtctgtcca cttgggtccc atgaaagatg
     7861 caagacatct ttgctagcag gtttttctgt tagtaatgga cctttgaccc attgtccttc
     7921 ttttgtaata aatacgagtt gaacttggta ataattataa taaatggcat ttaaaactga
     7981 aaatgcggaa agaatggaaa cttcatgctc tgcgctgcgt ccgccatata gtaaagtaat
     8041 cttcaaaaga ttgtcctcct gtattctaaa tcgttcagtg atttactctc atattttagc
     8101 acaaaacaaa aataaacgaa taacttattt ataaaaaaac gagttggttt ctaaaagaac
     8161 aaacgtcatt tattttttgt cagctagatt atttgtcccg ttttttataa aaagttatga
     8221 taaagaaaag gagggatgat gatggcgcaa acaaatccac gtagccgctt ggcagaaatc
     8281 attcgagtgt tcatcaatta caatgtagtg ccaaactttg tacagcaaaa aaatcccgaa
     8341 caggtaaaaa aagcctttga agaactaggc cccaccttca ttaagatagg acagatgctt
     8401 tctgttcgag aagatttgct ttcttctgcc ttcactcaaa cattcaaaac attacaagat
     8461 agtgtaccta gcgacacatt ttcaacggtc aaaaagacga tcgaaacaga actttctctt
     8521 tcattatcag atatattcga tgatttttcc aaatcgcctt ttgcctctgc ttctatgggg
     8581 caggctcatc gcgccaagct gaagaacggt gactcggtcg tcgttaagat ccaacaccct
     8641 aacatcgccg aagagatccg acttgatctt caactatttg aacgagcgat ccctttgatc
     8701 aaatacattc ctgaaactag cgtagttgat ctaaaagggg tactccaaga agtaaaacgt
     8761 tccttgatca atgaaatgga cttcttaaaa gaaagtcaaa atggcgaaca attctatcaa
     8821 aagaacaatg gctggaaaga gatccgcagc cccaagattt atgacgcttt ttgttcgaaa
     8881 aaagtgatcg tgatggaaga aatgtctggt aaaaacttga accatttaat gaacgccgaa
     8941 aacaaaactg aaacttttat cactggaatt caaaacaagc agctgaaaca agaagttgca
     9001 aagcttttag ttgagaactt tatgaaacag gtatttgatg atggtttttt ccatgctgat
     9061 cctcatccag ggaatctgct attccatgta ctaaaaaaag aagaacagac acaagctagc
     9121 cgaaaaactg aaatagtcca tgaaaaagag ttcggttcct ttgcttttcg agcctccact
     9181 tctacagaag accctgttgc gccatatacc atcaactaca tcgattttgg tatgatgggg
     9241 catctttctg caggacttag gcaaaaactc acacaagccg ttttagcgct gtatacgaaa
     9301 gatgcttatc gaatcgaaaa agcggtcctt cgactttgtc agcaagaagg ctcgtttgat
     9361 gaaagtcggt tccaccaaga actgacaagt tttcttgaac agtattatga ttctcctatt
     9421 gatgaaatca acttgcaaga agtattcaca cacgtcgtga cgatttgtca tcaaaataat
     9481 ctgcagttcg atcgtgatat cacgcttttg ctgaaagcat ttgggacatt agaaggggtg
     9541 atccgcgtcc ttgaccctga agtttccctc atggaagtcg cttcgccttt tgcccaacat
     9601 tattttctca ctcatctgga tgtagaagat acattgaaac aaagcggatt agacttattg
     9661 gaagggatga aggctgcgcc taagatccct caacagctcc accatctatt agaaatgtgg
     9721 acttcgggac aaggaaaagt caatttggaa ttgaaaaagc aagacaaatt attgagcagg
     9781 atcgaaagta tgatcaatcg gcttgttttc ggtatgattt tagctgcatt gatcgtaggg
     9841 tcctccctgc ttgtccaagc agcacctgta gaaaacgcag aggtcgtttc cttattaggg
     9901 atatttacgt atgctattgc cgcattcgtc attatttttt tggcaatcga tgcattgatc
     9961 caaatgtata agaagaggaa aaagtagaaa aaggttgtga cagaaagaat cagcttcaag
    10021 aaataagaag tgattttgca taaattgttt ctcaaatttt tgtgaaaatt cggcttattt
    10081 ttcgaagagg ctacttctga agcaccgaga ataaaaaatg tgaaaaagct gttctgcacc
    10141 cagtaataag aacaatcaaa aaggaacatg gagcacattt atcctgtttc cctgttccct
    10201 ttttgatgta ttcgagcata aataaaatac cttcgttacc tactatcttc tgtacttggt
    10261 aaaccttggt caattgcctt ttttaacgct tgacaaaaag acatatgatc tttctccgta
    10321 aagggttttg gaccctttgt ccttatgcca gcttttctta actttccact atgataacgt
    10381 tgttctagaa gcgtgccgat attcgcttcg tcaaattctt ttccagagtg atggaacact
    10441 cggacgccct tcggcaatac aagtgaagct aaaccataat cttgcgtgat caaaaagtca
    10501 ttcttcttaa ccataccgac gattttataa tccgcactat cagctccgcg gtctacgtag
    10561 acgatttgga catgattagc gtattctttg gtcgtataat ggtccacact ggtcacgata
    10621 accacgtcaa gaccatagga ttctcctaat tttatgacat cttcttttac tggagaacca
    10681 tctccatcaa tgaatagcct cattcttttt cgccttcttc cataaatgat aaacagttac
    10741 taatccgctc agtaaaagac caacattgat ccattttgcc aagataagca gttgtacact
    10801 ttctatcggc actgattcat ggttcagcgt gatcaataat agatggattg gctctcccat
    10861 ttcttttgct aaaacaaaca aaagcgtcat caataaaaac tggtggatca cactcccttt
    10921 attttgagta gtatacttga caacgatccg gcgatccatt ggaatagctg catcagtttg
    10981 ataatattcc ttatttagcc agctgcctgt tactacgtgc cagaaactaa gcagaaagac
    11041 acctatcgca aaaccatttg gaatcaagag gaccaataaa gaagaactca gtccaagcaa
    11101 ttgcatagcc aatggagacc atgttaaacg tttcaaaatc catggtccga ctatttttgc
    11161 tgcaatcaag cctgctgcat aaggcaaata cataaaaatc gaaagttttt ctaatccgta
    11221 cattatgcca acataaaaac tgctaaataa aaaaaggaaa ttccctagca ttccatttag
    11281 aaaagtaaac agatttagcc agcttggcaa cttccattcc ttcttctggt gtcctaaaac
    11341 aaacacgaac aagatgaaca gcagcaagaa accataaata gcaagatcaa aaagcctttc
    11401 atcgaataac aatcgggcat ttctcaaaag aaacaataag ccaaagaata tcaaaaataa
    11461 aacaagctct cgcgtagcta ccagatttct tttgatgtct ttgaaatcca gttcgtaacg
    11521 cggataatga ctaaccgtat ggtaggccat gacgtaaaaa agtgtataaa tagtaaagct
    11581 tgctgcaatc tgagtcggtg caggcaactc aatcgcttga tacaggagaa atagccagat
    11641 agctgcaaat aagtactttt tcccatccat attgataaaa ccttgcgtct tttcgtgaaa
    11701 attgaccgtg gtattagcag gtacaagcca tgctgcactc agccctagca aaacacttga
    11761 aaaataaaat aaaggaaaat aaaatacgcc taatattcca agcaatgaac cagcaccacc
    11821 aatcagcaga gcgatcatca gcaacgtgta actatctaga ccaaatcgta gactgttgac
    11881 taaaaatagt ccagtcatcc gaaaggcata aaacagcgta aatggcaaaa tcgtcagcca
    11941 attatgcgag tctgttagtc ccaaaaataa aagataagga ataaaaataa ccgtgttact
    12001 taagataaag ggcgttgttt gtgtaaaatt acgcccaaat cgtttgagtt tttcgaacat
    12061 gacaatggct ccttaaacag tcttataact aatctgctct tcgattttag cattaaaggg
    12121 gaaataaacc aattttacag aatatattct tgtaaaaaaa atacagaaag aagcataagc
    12181 ttctttctgc atttttacgg tacttgatta ctcaagcaga caagcccttc tccacaaaag
    12241 tcttgcccga cacccctgca tccggcaggt gataaagata gaaatcaatt atcctgtgcc
    12301 tccaaaatca attaggagct ctgtaaacca gagtccttct cgacccgtcg cataaattta
    12361 ctttaacaca gttcctatgt ttatgcgaga gcttttttca ttttataatc gaccatttga
    12421 aatccttgac cgatatctga aataagttct tctgtaatct caaatccttc atgcttataa
    12481 acctcgattg cttgatgatt cccctgattc actcgcaaaa agatctctct ttcctttgtt
    12541 tgcgcagcaa gttcttcata ccagcgaaaa actgaagaag tcaatccttg accacgtact
    12601 ttatttagca aatataattt acttaaataa atttttccat ttatctcttc ataagcagtg
    12661 taaccgacag tcttttcttc gctttttaat aaaaagtagg acacaccttc tgtcagttct
    12721 ttttgaatct gtttttttga ctgataagtt ttcagcatgt aatccacttg tttttgacca
    12781 atgatcggca caaaagcttc cggccatact tcttgtatga ctgccatcag ctcatccagt
    12841 tcattttctg ttacttttat tttttccata cacccacgtc cttttttaca ttataaatga
    12901 ccttgcgctt gatggaataa aaagagcatg atcatgctat acacacccac cgaccacggt
    12961 ttcaagaaaa tcacaatctg cataaattcc ctaaaagata atttcgtcaa accagctact
    13021 agacagatca agtcgtctgg cgcaaaggga agcagcaagg taacaactag tactttttta
    13081 atatctttcg tttttcttgt cagcaattca tcaaatttct gatacttttt tggcgataaa
    13141 ataagctgga caaaggctac gccatagtaa cggacaagca aaaatccgat tgcttcccca
    13201 atcactaggc caatacaact atataaaagc ccaaatccat tgccaaacat cagcatgccc
    13261 gctactgagg agatccctcc cggcaaaata ggaatgatca cttggatcac ttgcaacaga
    13321 acaaatatca tcactgcata cttgccaaat tgctggataa acgcttgcaa agacgtgctc
    13381 gatcgaaaaa tcccatgatg ataagcataa agaatcaaca caaagaaaaa aaacagacca
    13441 ataatgggca tgatctgaag acatttttgt aaaactgatc cttttttaga catctatatt
    13501 ccccacatct tttttcattt attaaaactg cctcct
//
LOCUS       DACKAC010000072        13504 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.073, whole genome shotgun sequence.
ACCESSION   DACKAC010000072 DACKAC010000000
VERSION     DACKAC010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.073"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(45..1364)
                     /locus_tag="I0B46_002263"
     CDS             complement(45..1364)
                     /locus_tag="I0B46_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAR2084525.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
     gene            complement(1361..2014)
                     /locus_tag="I0B46_002264"
     CDS             complement(1361..2014)
                     /locus_tag="I0B46_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAR2084526.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            2302..2822
                     /locus_tag="I0B46_002265"
                     /pseudo
     CDS             2302..2822
                     /locus_tag="I0B46_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2724..3440)
                     /locus_tag="I0B46_002266"
     CDS             complement(2724..3440)
                     /locus_tag="I0B46_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAR2084527.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(3564..3749)
                     /locus_tag="I0B46_002267"
     CDS             complement(3564..3749)
                     /locus_tag="I0B46_002267"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084528.1"
                     /translation="MTKALIFDFDGTMIDSEWMFYESIHHYLLKPIYWSSSAIKFLLQ
                     TINKVLGRQQNCPFMKG"
     gene            3976..5249
                     /locus_tag="I0B46_002268"
                     /pseudo
     CDS             3976..5249
                     /locus_tag="I0B46_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(5356..6387)
                     /locus_tag="I0B46_002269"
     CDS             complement(5356..6387)
                     /locus_tag="I0B46_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAR2084529.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(6394..7224)
                     /locus_tag="I0B46_002270"
     CDS             complement(6394..7224)
                     /locus_tag="I0B46_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAR2084530.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(7221..8027)
                     /locus_tag="I0B46_002271"
     CDS             complement(7221..8027)
                     /locus_tag="I0B46_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAR2084531.1"
                     /translation="MKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLANYS
                     DIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLAANF
                     AGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYPSMK
                     EVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALTSSN
                     VNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(8033..8857)
                     /locus_tag="I0B46_002272"
     CDS             complement(8033..8857)
                     /locus_tag="I0B46_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase"
                     /protein_id="HAR2084532.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(8847..9947)
                     /locus_tag="I0B46_002273"
     CDS             complement(8847..9947)
                     /locus_tag="I0B46_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAR2084533.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCVSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            10125..10910
                     /locus_tag="I0B46_002274"
     CDS             10125..10910
                     /locus_tag="I0B46_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HAR2084534.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            10943..11326
                     /locus_tag="I0B46_002275"
     CDS             10943..11326
                     /locus_tag="I0B46_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAR2084535.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            11402..11533
                     /locus_tag="I0B46_002276"
     CDS             11402..11533
                     /locus_tag="I0B46_002276"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAR2084536.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            11502..11738
                     /locus_tag="I0B46_002277"
     CDS             11502..11738
                     /locus_tag="I0B46_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAR2084537.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(11816..12787)
                     /locus_tag="I0B46_002278"
     CDS             complement(11816..12787)
                     /locus_tag="I0B46_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAR2084538.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
BASE COUNT         4110 a         2932 c         2337 g         4125 t
ORIGIN      
        1 ataagtctct atttttcaat aactattgca aaattatatc ttaattaaga aaatattttt
       61 attttaaaga taaaaaattc ttcatcctgc aatactttat attctatatt gtaattattc
      121 agaatattac ttacaatata taaacctaat ccattactat tttccttatt taaatcaaat
      181 ttaacatcaa atattttatc catattcgaa attttattgt taccgtatga attctctata
      241 tataaccaat cattaactat cccaatatta attaccccat ttacatcagt atattttacc
      301 gcattactaa tcaaattaga tagaataatc tttaaagctg tttttcctat ataaacattc
      361 tcgtctaata tataattatt gacagttatt ttcttttgat ttgctaatat ttcatacttt
      421 tttaatatgt cttctaatgt atcatttatt ttcaaatatt cttcatcatt attcaaattt
      481 tctatagaat gaactgataa tatttgagaa atattttttg ttaaactatc aacgatttca
      541 atacattcat taatataaat atctctttct ttatattttc caatattgta tttcatgttt
      601 tccagtatta ttttaagact agcaagaggg gttttaagtt catgggatgc acctttaaaa
      661 aagtcatatt ttaacttttc taattttaaa atttctttat ttttaaattc caaatcgtca
      721 attgttctta ataaagtaga atacaaatca ttaatttgtt gttttaattc tccaacttca
      781 tcattagagc taacttttaa actcattttt ttatcaagtt tcatcatttt atcagtaaca
      841 atttttattt cttgtatatt attttttatt gattttgcat aaattaaaga aataatagca
      901 gaaaataaaa ttgatattaa taaagaatat ggtaaaaatt taaggcttaa atctttcgca
      961 tctttttgca tatcagctgt agaaacaaat tgtagatttg tttttttacc atcattaagc
     1021 tttatttctc tttcttcaat tatcaaagaa ttactatcac tttctaaatt aacatttata
     1081 ttatctttga tttgtatttc attcttatta tttttttctt ttatgaatac ttttatttca
     1141 ctactcttgg aataaaactc caaagtttgt tctatatatt ttatttcttt tccattcata
     1201 ttactagaaa tttcatttgc catattatga atcttttctt ttctcgtctc caaatatgtt
     1261 ttaggaaaga taaaaaaaat taatgaatga actaatataa ttattattcc aagaacagaa
     1321 aatatttgta tgaacatttt tggaaatatc tttaattttt tcattttctc tccaatttat
     1381 atcctacatt tcttatagta gttatacagt ctaattgtaa tttttttcta agttctttta
     1441 tatatacatc tattactcga tcataaggag tttcttcgct atctttccat acataatcaa
     1501 taatttgcat tcttgttaac acttgtccat tattatccaa taaacatttt aatacttcta
     1561 gttcttttgc atttacatct atttcttcac catttatttt agctgtatag ctattaaaat
     1621 taaccgatag atccttatat tcaaacttct ctaaatgtcc ataattcttc ttaattaaag
     1681 aatctattct agcttttaag accggcaatg aaaatggctt ttctacatat ccgtctacta
     1741 aattagtaaa tgcatcaatt ttatattctt catcactaaa tgcagtcaag attagaattg
     1801 gtaaattgct tttctttcta atttctttca acacttctaa accatttata aaaggtatct
     1861 gaatgtctaa gataaccaaa tttatatcgc tattaaattt tgacaaggct tctcttccat
     1921 ctttagcttg aataacagta tatccaaatt ctgaaagata ttcacttatt ccctctctta
     1981 tcatattatc atcttcaaca gttaatattt tcatatatac ctccacttaa gcctatacac
     2041 tagatattat acagcatatt atggctctat aatatctagt gttggtgacg ccttctgttg
     2101 ccaatacttt ttcttttttt taataaatgc aaacagacac cttgaaatcc tttagaaaaa
     2161 aggcttctat aatattttgt gttgttggaa aatcgaaggc gattttctac taacactttt
     2221 tcttcgtttc aactcaaaaa gagactcact tttggtaaaa tttagtcgac caaaacatca
     2281 ccctaccaaa ggagtcgctc tatggataat catactagaa aattactcgg attaacagat
     2341 aaacatttat tttttgatga agaatggctg attgaaaaag aatataaagg cgttcaagcg
     2401 cagtttatta aagggagatt ggatgactca ccttcccatt gtgaacactg tggatcgatt
     2461 cgtattatcc gcaacggttc ctatacgaca cgtactcaga tgttgaaagt caaagaaaag
     2521 ttgactattt tagaattaaa acgtacccgt tttctatgct atgattgtgg gcagaccttt
     2581 tctgcgaaaa cggacttagt tgatgaacat catcaactaa caaaggaact gaagcaggcg
     2641 attctcatag aactgtacga aaatcaatca cgtaatctga aaatcagaag aagtaccgtc
     2701 ggctcaagcg atattggaaa ctcttactca aagattccac gacattggag ccgctcaaac
     2761 ggcattatca tcgactgttt aaacgaccaa ttagtcaaac agaaatcgtc gatgaaccgt
     2821 tgtgcacaat ggtgtcctga acgtacagcg acaccttcca tatccagagc agtcgccaca
     2881 tcacggggat ggatgccatc caaattaaaa gcaaccacgc cagtatgatg ttcaggatct
     2941 tgcggtccgt aaatggtgat accgccgatc gcttgtaatt taggtaaaac gtaggcaacc
     3001 agttcttgct cataacgatg aatattctcc ataccaatct cattcaagta atcgattgct
     3061 cctgcaatgt tcggagtacc agcttcaaat ttccatggca gctctttcca tgaactatcg
     3121 taaagattga caaaatctat catttcaccg ccgaattcga ctggctccat ttgttctaac
     3181 aattgacgtt tgccatataa tacaccgatt cctgtcggcc cgcacatttt atgcccgcta
     3241 aaggcgaaaa aatctgcatc caacgcttgt acatcaactg tcatatgcgg tgctgcttgg
     3301 gcaccgactg gactatcctc aactacaaca gcctgttcaa ccctcagccg tttcaagcac
     3361 aacaaataac cttctggatc tggtttgccg acctgaagat catcgatcgt cacaaaggaa
     3421 tcaatttttt ccttaatcat cggatgtgct tcaaaaaaat agtctagctc ccgcagttca
     3481 gaattgagac tactccgatt ttccagtgct gctgatccgc tgctcaaaat gttcagcatc
     3541 cactggtctc ccaaccgctt gacttatccc ttcataaaag gacagttctg ctgccgtccc
     3601 aacaccttgt tgatagtctg caaaaggaat tttatagccg aactgctcca gtaaataggc
     3661 ttcagtaaat agtgatgaat actctcgtaa aacatccatt cactgtcaat catcgtccca
     3721 tcaaaatcaa aaataagtgc tttggtcatc ttctttcccc cttacccaaa acaagcaaaa
     3781 gctgtgtcct gctctctcta cagtatcagc ataaaaactg ccgtctgaaa agtcaaaggt
     3841 gaaagaaatg tggcgctata atttctagga ctgatagtaa atcattctat cagtcctttt
     3901 gagcataaaa aaacaaaaca tacagaagct ccgttagaat tgaatcacca caacacaatc
     3961 aaaggagcgt tctgtatgcc tgatcttcat cttatcaaac tattactagg aatcaaagat
     4021 ggaaacattc aaatcactca tgtcgagaag aaaaaaatac attcaatcaa cagtatcgtt
     4081 gtttctgcca cgctcattaa aacgattcag ccctgtcctc agtgtaaaca ggtgaatcaa
     4141 catgggctga tcgttaagaa cggaaagaaa aaatcgctgg tccttctcaa taagtgtgcg
     4201 aatcaacgga cttatcttgc tttggcaaag caacggtacc tctgtcgtgg ctgtcacacc
     4261 tattttacgg cgaataccta cattgttgaa aaaaactgtt ttatcgccaa acaagtacat
     4321 tataaaatat tggaggaatt gactgaaaaa caagcaatga cgacgatttc taaacattgc
     4381 ggcgtttctt ggtcaaccgt ttcgcgaaca ctcgcttctc tgctccccat gacaaacgtg
     4441 aaaataaatt ggttgcctcg ctgttcattg gtagatgaat ttcgttcatt gaaaaatcaa
     4501 gtcggtccct attctttttc ttgtatggat ggcgatactg ggaaattact ggatattctt
     4561 ccttcacgta agaagaaaga tctcatttct tattttatgc aatttgaacg acgtgcacgt
     4621 ttaaacgtaa aaatactcgt aacagatatg aacgcctctt atgctttgct gataaaagag
     4681 tgctttcctc atgccaaact cgttgtggat cgctttcaca tcgtcaagca cctcattcgc
     4741 agttttaagg atatttgact atgagtcatg aaaagcttcg accgaaacga tccaatacaa
     4801 gccaaacact atcgacaagt gaaggcttta tctcgacttc tgatcaaacg acaggacacg
     4861 cttgtttatg ataaatggac aaaatggcgt aattttggtt gggcctattt aaccgaaagt
     4921 gaagtcgtag aacgtctact ttctacttct gatgaactac gaatagctta tgcttactat
     4981 caagaaattc ttcaagcttt ttatgacaaa gaagcggaca tcttctttca gcttgtaaaa
     5041 acaatgccca agtcggttcc aagagaactg catcatataa aaaaagcgtt tataaactat
     5101 gaatccggca ttcgtttagc cttagagttg ccttactcaa atggcaaaat agaaaatctg
     5161 catacccaca tcaaagcatt gaaacgaata gcttacgggt taggatgaga accagaattt
     5221 tcttaatcaa tcatctgatc acttattaaa cataaaaaaa catctaaagc agaaaatcaa
     5281 ttctacctta gatgttctta tatcactcta tcagtcctat ttgacaaaga gccagaaatg
     5341 tttgccgact atacactagc tttgttgtgc aaaatgctga gtatcaattt ccacagggat
     5401 tgctagaata tctttcagct gaatgtataa ggcatcgcta attttctctt cccgatcgtt
     5461 caaccgatcc actaaaattt ctttgccctc cagcgtttga aaaacatacc gttgaatgtt
     5521 gcctaaaata aatgattcct tccatagaac cggaatctta accccctgag gaaccggttc
     5581 aaattgaatc agctccggtc ttaaataata aatgcagcga ttgcccttag cggaaaactc
     5641 gtgggtatac tgattcagct cataccattc gaaacgatta tagttgccga taaattccgc
     5701 aacaaatcgg ctctgaggat tctgatacaa ttccttcgag gtggaaacct gagccacccg
     5761 cccagcttcc atcacaaaaa tccgatcact gattcgcatc gcctcttctt ggtcatgagt
     5821 cacaaaaatc atcgtcatcc ctagcgattg ctggatagcg cgcaaatccc gctgcagctg
     5881 ttttctaatt cgggcatcca aagcacttaa tggctcatcc agcagcagga ctttcggttc
     5941 agtgaccaat gagcgagcca gtgccacccg ttgtcgttga ccgccagaaa gaaactggac
     6001 atttttatct gcctgctccg tcatatccac caatgatagc atctcaaaaa ccttttcctg
     6061 aatcacctca ggactcagct tctttacctt caaaccaaag gctacatttt caaaaacagt
     6121 catagttgga aacaatgcat actgttgaaa aacaaagccg atattgcgat ccttcgtagg
     6181 cttatcggca atattctcat tgtccagcac aatctcaccg ctctttggtt gttccaatcc
     6241 cgtgatacag cgcagcaacg tagactttcc gcacccgctg ggacctagta aagtaaccag
     6301 ctcacccttt tcaatctgaa tttcatcaat agctaaaatc tgtttttcat caaaaaacag
     6361 attgagatca tgtatttcta aaaacatttt ccgtcacaac tcctttaagg gcttcttttt
     6421 cgactgcttc tcttccctcg tctgcttaag ggacagctgt tgcttttgtc gataagtcag
     6481 cataaaggta gttaaagcaa ttcccaccat aaacagcaaa tacaaaacca tcacagcgct
     6541 agccaaatgg ccgttttcgt tcatccgccg cagcatatag atgcgtaccg tctgaaatcg
     6601 accgccaatc aacaggttcg tcagcatgta ctccccaaaa accgaagaaa aaatcatcag
     6661 agaaactagg gtcacaccga tacggatgtt tggaaacagc acctggataa taatcgtaga
     6721 catcggggcg cccaaggttt ctgctgcatc cactaattcc ttcagattaa ttaagcgcat
     6781 ctggttgtta agactaagat agacaatcgg caacgccgta ataaacaatg cacctaccaa
     6841 cactacaaac attgggatac cagtttcagc atacgttcga agcaaagccg ttaccaaaat
     6901 cactcctggt aaagcatacg gcagcaggag cagcttttcc agccaacgat tcaaacgagg
     6961 gaaatacaaa tgaatccaaa taatggtggg aatcatcaac aacagaatcg tcaccaagac
     7021 cacacctgct aataaaagtg agcgacccac cgcagcaata aattccctgt ccgtcagcag
     7081 ctgttggaac cattgcaagg taaaatcact aggtaaaagc gattttgacc atttagtgga
     7141 gctggcatac aaaatcgttc caataatcgg cagcaagtaa tacaggacaa agcagaacaa
     7201 aatcggtttt attaactttt tcacgtctgc accaccttgc ctttcttttg aataatcaac
     7261 attgccgtta ccagcaggac acctaaagcg actgccaacg cactgcctaa attaggcttg
     7321 gcgaacacat ctcccgacac taaagccgca attcgaatcg ttaaaagatt tacattactg
     7381 ctggtcaggg catacgctgt ttcataggtc cccatgccat tagcaaataa gatgacaaag
     7441 gtgctggcaa tcgatggtcg caaaaaaggc aaagccacct tgaaccaagc atacgctgat
     7501 gaagcgccca gcaattgtgc ggactccagc cattcctttt tcacttcctt catgctggga
     7561 tacaaaaaca agacccctaa cggaatttgg aaataggtat acgtgatggt caatccccac
     7621 caagaataca aattgaagtc cgctagaaat ggcaaaatca ttttcagcac acctacattc
     7681 cccaacaaaa taataaaaga gaatgccagc ggaatcccag caaaattagc tgccaaattg
     7741 gaaacaaagg tgattttctc ctgcaattta gtcgatagat tcattaagca taaacataaa
     7801 ataaacgtcc cgattaaccc aatcacactg gagctgacag caatcagtac gctgttaaca
     7861 aaggcctgtg tgtaatattt gttgctgaaa atatcactgt aattggccaa tgtccactgg
     7921 ttagttaaat cactgtgcaa gctgcacaga accataaaaa gcagcggcag cagtaaaaat
     7981 gcacagaaga taaaggaggc aggaaccaga accaagcgtt ttttcatctc tttcatcccc
     8041 tcatcaattc ttgaatgtcc tgcattttct ctgacggtgc aattcctaat aaccagcaaa
     8101 acaatggagc cgccagcagc tgtggtacag tttgttcctt cagcttttgg cgtttatttg
     8161 aaatcaaata aaaaggaact tcacgatgtg cagccaagct gccgccatgc agtccgtgcg
     8221 catccatccc gtgatccgca gtcaccacga cttgataacc tttttgcagc cattcaggaa
     8281 ggtaaatagc caataatctg tccgcctgat taaccgctgc tgcatattca tgagaatcgg
     8341 cggtaaattt gtgaccgaca tcatcaatat tcatcgaatg tacgaccaaa aagtctggct
     8401 gatactgatt cactaaataa tgggcatccg caaataaatg cgaatcaggg taagtgtcct
     8461 cactataaaa gatgccatgc tgaatcagtc gctcctcctg atgctggatg cgatcaacca
     8521 aacgattata gggggcttga ttgtacaact cgctgtacca gtggtaagcg gcggctcctg
     8581 tttttccgcc agcttcctta actaattcaa acagcgacca ctctttggat cgctgagcat
     8641 ggtaattact atagatgcca ttttcatagg ctggaacgcc ggtcatcagt acttcgtata
     8701 acggacgtga attgctgggc agctcacttt ttaccttcaa taagcttgct tgctggtgct
     8761 caactaaatg attcatgaac ccaagctggg cgacggctgt gtcgtagcga cagccgtcta
     8821 gtataactac aatcagtttg tcaagtctac tttgcatagg tcagtacctc cgcctgccaa
     8881 agctctggaa gctctacaga ggtcttgttc caagcttcat tgtcttcaac tggcaccgcc
     8941 gcttcataag catcctccgg cagtaatttt tcagctactt ctttaggcag ctctaccttg
     9001 cgaataggtc gcgcatagcc ctttgccaaa ttgatttgcc cttcatcaga taaaatatat
     9061 tcccgtgcta gctttgccgc attgggatga ggggcatttt tattgattaa ggttgtatac
     9121 ccgctaatga tggagccgtc tgttggcaca gttacctcaa aacgctcgga gtcaatttgg
     9181 tcacgataat ttagtccatt gaagtcccaa acaactgcca catcgatttc acctttttca
     9241 agatttgcta cgctggcatc aacggtggac aaacggcctt tttctgccat ttttttgtag
     9301 taattcagac ctggctgaat atccttttcg tcacctccgt tggcaatcgc cgcagccaat
     9361 acagcaaatt gtgcttggtt ggcaatcgtc acatccccca cgctaacttg atagtcaccc
     9421 tcttgcagct ccttccagct agtcggtgct tgcttcacat tctctttatc ggtgataaag
     9481 gaaatggttc ccgtatagct taacaaccaa tccccatcat catcctttgc ccaatccgga
     9541 atttcctccc aatagttggt cttataaggt aaggttaact ctttgctttc agccaacgga
     9601 ccgaagctga taccgacatc accaatatcg gcggttccat tttttccttc tgattcaaac
     9661 ttagccaatt cttctgcact ggacatatcc gtatccacgt gacttaagcc gtattctttt
     9721 tcaaggtcgt tccaagtgcc tacccaattt gcccaagtat ccggcattcc caagctggca
     9781 atctctcctt cttccttggc tttttctata atcgtatcta aagagtcgtc tgccgcagcc
     9841 gattgcttcg ttgcttcacc tgagacgcag cctgtcagca ggatacttac tgttgctgca
     9901 caaaataaac cgatagtcat gttacgaatt ttttcattca ttttcattca ttcttctccc
     9961 ctttcgataa ttccattcta acaaagcttt gtaatgagag aatcagtttc ttgaaaaggt
    10021 ttcatgaaca atgtaaaggt tgtatatggt ttgtaaattt cgacgaattc ccgttaacct
    10081 tctaaagctt ttgcggtatg atagtagaaa aaaggaggct ccctatgcag tattatgatc
    10141 agcatctcca cacgtatttt tcctttgatt cagaggaaaa atttgaaaac tatttagcct
    10201 atcagccgga gtttttcgtt tctacggatc actttgattt aaacaatcca tctaccggaa
    10261 aagatgacat tccggattat ccagcctata taaacaaact ggactcttta aaagaaacct
    10321 attccaccaa atttctgcga ggtatcgaga ttggcgttgt tccaggacaa gaagagcgga
    10381 tcgtggatta cctcgctcag catccttatg atttaaagct ggtaagtatt catcaaaatg
    10441 gccgctttga ttatatggac gatgtggttt taaccaaaga tcccattcaa actaccaagg
    10501 aatattttga tcagatgttg caggtcttga cggattttcc agaaggagat attttgactc
    10561 actttgatta tggattacgc cgcttcacct tcacagcgga agaattcgcg gagcattttg
    10621 aggctcagct gctaacgatt ttcaaaaagg ttgttgaacg agaattggcg atggagttaa
    10681 atgccaagag cttcttgaaa tacggcaacg aagaccttta ccgttatgcc gttcctttgt
    10741 atcaaagtgt cggcggaaag ctctttacct taggctcgga tgctcatgtt gccgaagatt
    10801 atcagctggg ctttaaaaaa atgagtcagc tgttggcaga ctgtcaggta gaaaagttgc
    10861 tagtcattca aggaaaagaa cgccgcctga cgccgctgcc gattttgtga ttcctatata
    10921 atagaagaaa gtggtgaaaa aaatgagcaa cctgtgggac tacaatcaag aggcacccat
    10981 ccattattta attgcgcgac attgggatgc gttgaaaatt gaggcggttt gccgatcctt
    11041 gttagcagcc gttccaaaac aacaactgga aaattttttg gtggctgaca gcttgcagcg
    11101 ggaaaaagtt caggcttatt ttgcagcctt taaagatcag cctttggaat atttgcatgc
    11161 gcagtttcat cttttttatc aagtagccgc cccagatgac tacaacgatt tacgaggaca
    11221 gctgcaattg acctttcaag cggatgaaac cgcctatacc gttttattgg gaatggctcg
    11281 ccttggtgat caggcgaagg tcgagtggcg gattttcgat atttgatggc aggatcaagg
    11341 cgtttcagtt gcaaaccttc ctctctttca attagaaaaa ccgcagcagg aagaagtttg
    11401 tgtgctggtc accgatgctg gtgaaattcg tctccgctta tttcctgacc aagctcccct
    11461 ggctgtagcc aactggcgct ccctagctga gcaaggcgtt tatgacaaca cacctttcgg
    11521 ctcgtgtcat taaggaattt gttattcaag gtggcgcttt ggatggttct ggtgatgaag
    11581 ctgtttccag ctatgagggc tttttcgctg atgaagtgca tcgcggactt tatcatttta
    11641 atggcgcctt ggccttaggg gaccacggcc cccacaccaa cggcaaccag ttttttattg
    11701 tgcaaaacac aaaggcgcag gctgacctgc taatgtaaag caagcatact tggagcttgg
    11761 aggactaccg gagctggatg gtcgttatac tgtttttgga caggtatacc aagggttaga
    11821 aatcgtagca gcgatcgcag ctcagccgac aaacagtgaa gatgtaccga ttgagccagt
    11881 gttcattcga caaattaact tcgaaaaaac atcgtaaaaa gacactcact gacttcagca
    11941 aaagctctat tcttgagctg gctgcagcta gtgagtgtct ttcatttatc cataagataa
    12001 agcttggatt caataaataa ataaatgttt tggttaaagc tgtgaccagc aaatgaggaa
    12061 ccgtgcgagc cagttgatct gccaatgcaa acatttcttt aggtgaaggc gctggcgtgt
    12121 ctgccaacgg atactccagt cccaatgctt ggtaaccgtg cataccatag cagtggtacg
    12181 gtagtaattc taacggtacg aaccgatcaa gatctgccac aaaacaagca atcgtcagca
    12241 gctcggtcag gttcatattg atacctggaa ccacaggaac tcgaattcgc acttcgcagt
    12301 taggatactc aattaaaaag cgacgaatat tcttcaaaat ttgttggtta tctaagccgg
    12361 tccattgttt gtggagcttg ctggtaaaca tttttacatc cacaaacaat gtatccacat
    12421 agggagctac cttttggatt tcattataag caccacaaaa gctggtttcc agtacggtat
    12481 tgattccaat gtatttcgat ttttgcaaaa tttctttagc aaaatccgcc tgaaccagtg
    12541 cctcgcctcc gctaatggtc actccgccat ccgagtgaaa gtaaaaaaca gcatccttct
    12601 caatctcatc cattacctct tcagcggtca tggtctggcc aaatcctttt tcaatctgct
    12661 tgctttgtga ttcgggaatc gaacaccagg cacagcgtaa tgggcagccc tttaaataaa
    12721 caacggtccg taaaccagca ccatcatgta gagaaccctg ttctattctt aatactgttc
    12781 cttccatata gatttttgaa ttacacacta gtttaaacta aaacacacaa ggaaaaactt
    12841 accatttaat cataaaatta gctcatataa atatactagg atgtaaaata gactggtcaa
    12901 aatctggtca aaaaagagga ctaatacagg tagtcctctt ttttgttatt ttaatattct
    12961 aaatcatttt ttattggttt attcccttat tcgatgtaga ctcaaatcac tatatcacta
    13021 gatatcaaac ggcgtcatac ggaatcaaaa acatcaaatt tgacctgtta aatggtcatt
    13081 gaataacatt caaaatcaat aaaacggagt cagacacgga gtcaaaaacg ctgagagtaa
    13141 aaaatatata cagccttgta ggtaaagtac cacagggctg tatatttttt atttcagatt
    13201 accttaaatg attccattta ctttttatct cattttttcc tactagttag tatcaaatac
    13261 tctcctattt tctcaataaa ctatttttgg atacatggta tctgaaacag ctaaaacctt
    13321 gtcattacag caaaaaatca cgttatccag actgtagaca aagtccattt tatggatgat
    13381 gtctacagtc ttttctttta taatagattt atgaaaacga atgcaaggag tgatagctat
    13441 gatgtctaaa aacaatacga atggtagaaa tcaatttgcg atgcttacga ttgatgatct
    13501 agtt
//
LOCUS       DACKAC010000073        13147 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.074, whole genome shotgun sequence.
ACCESSION   DACKAC010000073 DACKAC010000000
VERSION     DACKAC010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13147
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.074"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(77..149)
                     /locus_tag="I0B46_002279"
     tRNA            complement(77..149)
                     /locus_tag="I0B46_002279"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Lys,seq:ctt)
     gene            530..1099
                     /locus_tag="I0B46_002280"
     CDS             530..1099
                     /locus_tag="I0B46_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAR2084539.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1086..1946
                     /locus_tag="I0B46_002281"
     CDS             1086..1946
                     /locus_tag="I0B46_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAR2084540.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2136..2840
                     /locus_tag="I0B46_002282"
     CDS             2136..2840
                     /locus_tag="I0B46_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAR2084541.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2845..4680
                     /gene="walK"
                     /locus_tag="I0B46_002283"
     CDS             2845..4680
                     /gene="walK"
                     /locus_tag="I0B46_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAR2084542.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4677..5993
                     /locus_tag="I0B46_002284"
     CDS             4677..5993
                     /locus_tag="I0B46_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084543.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5994..6863
                     /locus_tag="I0B46_002285"
     CDS             5994..6863
                     /locus_tag="I0B46_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084544.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6918..7727
                     /locus_tag="I0B46_002286"
     CDS             6918..7727
                     /locus_tag="I0B46_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAR2084545.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7830..8459)
                     /locus_tag="I0B46_002287"
     CDS             complement(7830..8459)
                     /locus_tag="I0B46_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HAR2084546.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8629..9921
                     /locus_tag="I0B46_002288"
     CDS             8629..9921
                     /locus_tag="I0B46_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAR2084547.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9997..10659)
                     /locus_tag="I0B46_002289"
     CDS             complement(9997..10659)
                     /locus_tag="I0B46_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAR2084548.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10884..11168
                     /locus_tag="I0B46_002290"
     CDS             10884..11168
                     /locus_tag="I0B46_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAR2084549.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11219..12844
                     /gene="groL"
                     /locus_tag="I0B46_002291"
     CDS             11219..12844
                     /gene="groL"
                     /locus_tag="I0B46_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAR2084550.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4370 a         2265 c         2833 g         3679 t
ORIGIN      
        1 taaaaaaaga acagcgtaaa tgttttcgaa atgctttcgc accaacattt acctgttccg
       61 tgggtttggc acccaatggg ccgtgagggg ctcgaacccg cgacccgctg attaagagtc
      121 agctgctcta ccaactgagc taacggcccg tacaaaaagt acttaataat agtagcacta
      181 agaattttaa aatgcaagac tacatttaaa gaaaatcaga aaaatcttta cttccttata
      241 ggaaatcatt catagattat tcacatttgc tcgctattat ataagcatac caacaaacaa
      301 agttcttgca agagaacaaa ccctaacact tttttcattt tttactcctt tcccgccgtc
      361 tccccggcgg gtctcctgtt cttaacgata aaagacaaga aggcttagcc ttcttgtttt
      421 ttgttttcat ggaatcccgc taatcaaatc acctagttaa agcaactaga ttgaatgttt
      481 atcataagtc tgatatcatg tttatgaaat gaaaaaagga gcggttatga tggaaaaaat
      541 gcgtcagcaa ttaatggagt gggggaagga actagaaaca ttccgtctgc ctcactggga
      601 agaactccct gacttagaac tttatatgga ccaagtcaga acgttagttg atcgttatct
      661 ttcacctgtg atacaaggag aaaagcatcc attgcttacc tcttctatgg taaacaatta
      721 cgttaaacta ggtttgatac cagcaccagt taaaaaaaga tataacaaag aacacgtagc
      781 atttttactt gcaatcacta cgttgaaaca agtattgact attcctgaaa tcaaagaagg
      841 gatattgttc caaggaaaaa cagtaggaat tcgtgaagcc tataatctat tttgcgatga
      901 acaagaagca gctgtgtgga tggtcagcca attagcacaa ggtaaatctc atccgcaaaa
      961 attcgaccag gcaacccctg tagaatatat tgctgtaaga gcggcaactt tatcgtttgc
     1021 aatgaaactt cttgcggaaa aaacgattgt tctagaaacc gaatatttaa aagaggagaa
     1081 aacaaatgaa aaacaataaa atcgcaattt tagtagattc aggtacggat gtgccagaag
     1141 aattgataaa aaaatacgag atgtatatga tcccattgaa aattatttat aaagatcagg
     1201 tatatacaga taaaattgac attacctctg aagagatcta tcaacgtttg ccagaggaaa
     1261 tccctggtac atctttgccg gatggtgagt cgatcacaga aatctttgag caaatcaaaa
     1321 aagacggtta tgaaaaggta cttgcggtaa caatttccag tggattgagc gggacatata
     1381 atgtggttcg tttaattgca caacagcaag aagaattgga agcatatgta ctagatacga
     1441 aaaatattgg tattggtgca ggtttttctg cgatccaagc agcaaaatgg ctggaagaag
     1501 gagtagaatg ggatcaactg atcagtaatc tgaatgagtt agtcgaaaga acaaaagttt
     1561 tctttaatgt tgcgacattg gaatatcttc aaaaaggtgg acggatcggt ttagttgctt
     1621 ctattgtagg aaccgcttta aagcttaacc caatcatctc atgtaatgaa gaagggattt
     1681 attacacagt aggaaaagct aggggacgta aaaaaagttt ggataaaacg gttgcttacg
     1741 tcaaagaaaa aatcggtaat gcaaaggtct tcaacttagc tgttgcccat ggggatgcga
     1801 aagaagaagc aattgaaatg gaagcgagac ttaaaagaga gttcccacaa gcaaaccaaa
     1861 tctactttgg ccagatttct ccggcattag tagtccacac tggtccggga ttattgggtg
     1921 tcggtgtaca agttttagaa ggataaatca aaaaaaggaa acactgaggt gaaaatcagt
     1981 gttttccttt tttattgaag ttagaacaaa aaataataaa gtattttgca ctttcatttt
     2041 aattttctct tttgttttat aggcaaagat gaaacaaaag cagaaatacg gtataatgag
     2101 aaggtatgac aataatatat aagaggtggc tctttatgaa aaaagtttta gttgtcgatg
     2161 atgagaagcc gatttcggat atcgtcaaat tcaatttagc caaagaaggc tatgatgttt
     2221 atactgcata tgatggggaa gaagccttgg aaaaagttgc tgaggtagaa cctgatttga
     2281 ttttacttga tttgatgcta cctaaaatgg acggcttaga agttgctcgt gaggtacgca
     2341 aaacttatga tatgccgatc attatggtaa cagccaaaga ttctgaaatt gataaagtcc
     2401 taggcttaga gttaggcgcc gatgattacg tgaccaagcc attttccaac cgagaattag
     2461 ttgcacgtgt caaagcaaat cttcgtcgcg gtgccacggc tgcgaaggaa ccagaagagg
     2521 cagcacctgc ggaattgacg attggtgatt tgacgatcca tccagaagct tacatggtga
     2581 cgaaacgagg agaaacaatc gagttaacac atcgtgaatt cgaactactc ttttacttgg
     2641 cgaagcacct tggacaagtg atgacaagag aacatttatt acaaacggtt tggggttatg
     2701 attattttgg agatgtacga acagtggatg taactgtacg tcgtttgcgt gaaaagatcg
     2761 aagataatcc aagccatcca aattatttag tgacacgtcg tggtgtcggt tactatttgc
     2821 gtaacccaga acaggagtaa ttggatgaaa agaaaagtcc gcttttttcg atcagtcaat
     2881 tttaagatcg ctattacctt tattctgatt ttattgatct cgatcgaaat tattggtgcc
     2941 tactttatac gtggtttaga acgttccacg atcaatacat ttattaaaga tatgaatcaa
     3001 acggtggaat cattagcaac aacaatcagc ccggaattga atcgaaaaga taatgcagat
     3061 gatgaagagg taaatgcgaa tatcaaaaga tttattgaaa acagtgcgac tagcgatatc
     3121 atcgaaatac gggtcgttga cgaaaaaggg atcatccgag gaacaaccga tgtcaatgaa
     3181 caaagcgctg tgggaaagaa aaatgattat gttgatatca atgatttcac gactaaacgt
     3241 tatgttgctt tagataacga cgataaacgg gtgaatatca atgttcaacc gatcctgtcc
     3301 ccgaccggcg atgccgttat cggggctctt tacgtcaaaa gtaatatcga acaaaaatat
     3361 tcggaaatca ataacacagc agtcattttc tttactgcct cactaattgc ggcatttatt
     3421 tcaatgatcg tatctgtttt agttgctcgt tcgattacac agccgatcgg tgaaatgaga
     3481 gaacaagcca ttcgaatcgc tagaggagac tacagtcgga aagtcaaagt gcatggacaa
     3541 gatgaacttg gacagctggc tgataccttc aatcagttag ctgaacgtat tgaagaagct
     3601 caagatacaa tggaagctga acgcaatcgt ttggatagcg tgctatccca tatgacagac
     3661 ggcgtcattg cgacagatcg caggggtaaa gtcattacga tcaatgatat ggctgtttcc
     3721 ttgctagata tcaaaaacga ggaagcaatc ggacaatcga ttttgacact tttagatatc
     3781 gaagaagaat atacgttgcg gaaattatta gaagatccaa acgaaatggt acttgaccgt
     3841 tcaaccagta cattggaaag tgatcagatg atcttgcgag tagacttttc tatgatccgt
     3901 cgcgaatcag gttttatcag cggattagta gctgtcttgc atgatgtgac agaacaagaa
     3961 aaaacagaac gggaacgtcg tgaattcgtc tctaatgttt cccatgaact tcgtacaccg
     4021 ttgacaagta tgcgtagtta tatcgaagca ttgagcgaag gtgcgtggaa agacgaggaa
     4081 gtagcaccaa attttttgaa ggtaacgcta gatgaaacag accgaatgat tcgaatgatc
     4141 aatgacctct taaatctatc tcgcatggat acaggtaata cccagcttca attagaatat
     4201 gtcaatttca acgagttagt gaattttgtt cttgaccggt ttgatatgat ggtgggaaat
     4261 caggaaaaaa attataagat ccgtcgtgaa tttacgcaaa gagatctttg ggtcgaagta
     4321 gatacagata aaatcatcca agtagtcgat aatatcatga ataacgcgat caagtattca
     4381 ccagatgggg gaacgattac ttgtcgctta ttagaaacgc ataataacgt ggttttaagt
     4441 atcaccgatc aaggattggg gattcctaaa aaagatctga accgagtgtt cgagcggttc
     4501 tatcgagtag ataaagcacg tgcaagagca caaggtggga caggacttgg attagctatt
     4561 tctagagaag tgattaaggc gcatcgaggc gctatttggg cagagagcaa ggaaggaaaa
     4621 gggtcgactt tttatatctc gctgccatat gaaccttatg aggaggaatg gtgggaatga
     4681 gaatctctga aaaagtcgtt cgtataggac tcattttgat ggttgcgtta agcatatact
     4741 tctcttatgc aatctggctg agcccagcag gaaaaagttc aatcaacgta gatgagacaa
     4801 acactcaaat gatcgagtct caaagcaatc gaaaaacttc cgaagtcttt cttccgcttc
     4861 atgttacatg gcaccattcg ggaatgatcc aagaaaccaa cagtgaaaat ctcgtgtacc
     4921 gattgcaatt gatcatcgat aaagcaagat ttggaaagct ttctgaagta gtttcaggag
     4981 acaaatcaca gtttgaacag gtgaaaaatc tgaactctgg ttttgagttg gcctataatg
     5041 cgcctttttt gctaagagaa tacaaggaag catttgattt agatttagat gtgtcgaact
     5101 tggcaaaaga aagcgaaaat atgtatttta ctagaataca attcgatcaa ggaaataaaa
     5161 aaatccgatt tttgaattat tcgaaccatc tcgtatatga agcaaatatt tcgttgaatt
     5221 ggacagatat caataaagaa ttaacagcaa aagacacaaa atggacagaa atgacaacag
     5281 aaccatcgac gatcgacacg caatatgcta cgaaacattc gatcaaactg aaaaagtata
     5341 gttatatcct gtcacagcaa ccctatacac tttttagaaa tgcttttttc cagaaaccag
     5401 atagcgtgaa aaataatgat gaaagcaatg aattgatttt ttacggtggg aatgaaacaa
     5461 tgtctatcca ttcggaaacg caagtagttg attttcgtgg agagctgcca gagaaaaaag
     5521 aaaatgctag tgtattttct gaaagtttcc cctatattag caaattaggc agttctttag
     5581 ggaacctcag atattttgac cgcagtgaca atacgatcga ttatcggata ttcgtggaag
     5641 gcttcccggt ttttgggaca gacagcagag gacagattgg tgtggaagtt ggcggtgaaa
     5701 cgaatggttc tgtgcaagtg aatatccaaa ccagtttgaa tacgattcaa gtgccgatac
     5761 cttcagacga agaagtagaa cttgcaagta cgaatgaaat catccagcag ttaaaagcta
     5821 agggagcaga tagcaacaag attggttcag tgatcgttgg ctatacatgg caaaatgtca
     5881 aagaaacgaa tcgtgtcgtt gatctcttac ctgaatggta tgtgaaatat aaagataact
     5941 ggtattcagc taacgacctt ctggaaaaat tacctggatt ggaggcgaat taattggatt
     6001 tcaaaaaaat cgaatggata tttttcgtcg cttttttagg gttgaatgtg tttttattca
     6061 gcagttatca tgacgcggta taccaagaaa acaatgtcat tcgctctaat cagactgaat
     6121 caatccaaca acgtctggct agcgacgata tcacttacac ggggaagatt tcgtctgaga
     6181 acaagcaagg ctattatctt agcgcggagc agaccaatct tagcacttca ttagcgaatt
     6241 atcgcaaggc tggtaaaagc ggcttgctgg caagtggtgt gtttttagac ggtgatgttt
     6301 tgacgaaatc tttatccagt tcagatgctg aaaagaacgt gatcgatcca gataaaatta
     6361 gtagttcctt ggaagatttt ttatctaaca aagacaatat tttattcggt aatgattatg
     6421 tgtacacgaa aaacttttct catttagaag aagaaccact agaaatccaa gcaagccaag
     6481 agtatgaagg catcccattc aatgatgata cagcaaaaat cgttctttct ttagaaaaaa
     6541 acgataatga ttatagcatc acgaaataca tgcagacgca tttatctgat atcgagcagc
     6601 tgcgcgaaaa gacggaactg tatacggaag aggaagcaat caatacattg tacatcaata
     6661 acaaaatacc aagaggatca aaaattttat ggcgtcagct agcttattcc tgtattctga
     6721 aagtccggga aaaaaatgtg tatgttcctg tttggcatgt agcgatcgaa acaagcgata
     6781 aagtggtcca aattgagagt gtcaacgctt tcagcaatac gatcgtgaca aacaatactg
     6841 taccaaaagt ggaagatcaa taaaaaagcg agtataatga aaaaagttta caaagtaaga
     6901 agggaagtag gagtcaaatg gctgaagaaa aaccagcttt taaaatcagt atcctagcaa
     6961 gtggaagttc gggtaattca ctttatatcg aaagtgaaaa aaaacggctt ttggtcgatg
     7021 caggacttag cggtaaaaaa atcacttcct tgatggctca aattgatcgg acaccggacc
     7081 aattagatgg aatacttgtg acgcatgaac atcgagatca tatacatggc gtgggtgtct
     7141 tggctcgtaa gtatcatttg gatgtttttg ccaatcagca gacttgggaa gcgatggaac
     7201 cactgattgg aaatgtacca gtggaacaaa aacatctatt tgagatgggg aaagtcagaa
     7261 cattcggcga tatcgatatt gagagtttcg gcgtctccca tgacgcagcg gctccgcaat
     7321 tttatcgttt ccataaagaa aatcggtcat tcgtcatgtt gacggacacc ggatattgca
     7381 gtgattatat gcgaggtgtc attgaaaatg ccgatggtta tttgatggaa agtaatcatg
     7441 atcttgagat gttacgcatg ggaccttatc cgtggaattt aaaacagcgt attttaggag
     7501 atagaggaca tctttccaac gaagacggtg cacttgtcat gactgatgtg atcggtaatc
     7561 ggaccaaacg aatctatctt ggccatctta gcaaggaaaa caatatgaaa gaacttgctc
     7621 atttaacaat ggaaaatgtt ttgaaagaaa aagattttgg cgttggacat gattttgaaa
     7681 tctatgatac agatccagat gaagcaactg agctattttc gatttaaaca gttcaatcaa
     7741 taaaaatata aaaagcagaa gataaaatca atcagggttg gttttatctt ctatttttat
     7801 ttttttagca tattaaaaaa ataaccgtat tatcgactga ctacactgat gtgctgcttt
     7861 tcatgtttct catccagtat ctcatctact aatgcaatcg cgtaatctgc catgctgatc
     7921 gtactgtcgc cagcagcgtt tctttgtaaa taatcatcac ttaacaaata agtacctgta
     7981 cgtttgccat gtggatcaaa taaagaagct ggactgatat aagtccaatg tagattttta
     8041 gattgtttca attttaacaa tccttcgtac atattgaaag ctgttggata gaagggtgca
     8101 tttttaggta cttggtcaat catacgagtc gtcttttctg tatctaagaa taacgaagag
     8161 gcacccccaa cgacaattaa gcgtgtatcg gttccttcaa gcatctcaat caaatgggac
     8221 aaagaagttt gatgcatttc ttcttttcct ttgggagcgt tgaaagcatc aacgacaaca
     8281 tcaaaagatg caagatcagt agttgtcaac tcaaataaat ccttttgtat aaacggaaca
     8341 tctgatggca atttttctgg atgacgcacg attgccgtca cgtctaaatc tctggacaat
     8401 gcttcgtcta aaataaatga acctgcatgg ccagtagctc caatgagtgc aattttcata
     8461 aaaaacctcc ttttttattc ctgtccattg taagcagttg ctgaatattc gtcaaagaat
     8521 ccgatttttg aaaagctcag tgcgcattcc tttcatggtt cgtgatcatt gggtatagtt
     8581 aaattaagaa aactgattga taaaaataga agttgaagga gataagaaat gaaagagtgg
     8641 ttagatcgtg cctttttacc aatgataaca tctgttgtga atcttagacc cttaactgct
     8701 ttgcgtaatg gaatgactta cacgatccca ataattattg ttggctcgct gtttttctta
     8761 tttgctcatt ttcctgtgga ttacatcgct cagtggatag acaaaacagg attttctatg
     8821 tattttacac aagtatacca ggctacattt gatgtgatag ccttttggat cgtttttgcc
     8881 attgcatacg cttatgcaaa agaagaagac atcgatcgct taccagtcgg cataacagcc
     8941 ttgtctgctt atctactgct tattcagcca ctaaatactc agatagatac tgtctggaca
     9001 ggtgctgaag ggatactagg agcagttaca actgggctca tcatcggatg gggctatgca
     9061 cgtttagtaa gacttgcgaa agggtggaag caaagagaga agatacctga aaatgtttgg
     9121 cagtctttta cttcattgat tccaatcatt gttatagtca cttttacgct tatcggttca
     9181 gcggtttttc aacagttgtt cagaatgact gttgttcaag gagtatggac atatgtccag
     9241 cttcccattc aatcgttcat agacacgttg tttggtgtgc ttttattagg tttttttgtt
     9301 ccgtttttat ggttatttgg tcttcatgga tctgcgatgg tcaatgggtt ggttagtccc
     9361 gtcttgcaag cgaattcatt agcgaatgca gaaatcttag cctcaggaca agagttgacg
     9421 gtggcaaatg gcggacatat cgttactcag caatttctcg atcagttcat gacggttacg
     9481 ggtgctggat tgacgttagg ggctgtattt tttatgacgg tttttgcgaa atcgaagaaa
     9541 tacagagaat taggaaatct tgctctttta ccagcctttt tcaacatcaa tgaatcaatt
     9601 attttttcca caccgatcgt gatgaacccg atgatggctg ttccttttat tttcgcaccg
     9661 gtcgtttcag gattgattac ctattccgct ttatattttg gattcgttcc tttatttaca
     9721 gcagtccaag tcccttggac aacaccgccg atcctctcgg gtttcttagt aggcggagtg
     9781 cctgcagcga ttttacaagg tatcgtgttg tcaattagtt ttttcattta tttcccattt
     9841 ttgaaaaaaa tagattctgc aaactgcaaa aaggaaagac agacaaaaaa tgatggagca
     9901 gcagaaaaga tgaacgacta aaaaacaaaa atactcctgt cgctttagat tgggaaaatc
     9961 accaatctag gatgacagga gtatttatag tcatgtttat actagccctt tttggacagc
    10021 taattgaaca agaacaacta atgtgttcat tcctacgtgt gtgatcatcg atgtccagat
    10081 ccgaccagtt gctttgtact ctaggctgaa gaagaaacct aggaaaaagt agattagcag
    10141 atgaccatca ttatgagcaa acgcaaacag caaagagctg atcactgctc cgacccaaaa
    10201 atttgaatat cgactgatga tccccagtat actgcggcgg aatacgaact cttccatgat
    10261 gggtccagca acagttgtcg ccaaaataaa agcgggtgct tccatgatca tctggatgat
    10321 attttgtgta ttctcggaag gggttgcttt gccaaacaat acagattcta acatgacagc
    10381 gatgctttgc agaatcaatg cgacaaagat tccaaccaat ccataaataa caatctttgg
    10441 agcagaagct ttttttaccg ctgattcgat cgctaagggt tcacgttgct taaaataaaa
    10501 aatagctagg acaacggcgc ctagtatata catgactgca gttgctgtga tcaattcaga
    10561 cgtagatttt acaagtccta tcgaagctag gatcaagggt gagataaaaa ttagaccata
    10621 gcaaaaaatg ctgaggaagc tatatttttt taaagacatg taattcctcc ttttaaggga
    10681 taacaagcat gcaaggcaag cttatatcga ttgatttcaa ttatatcata atttcaataa
    10741 gcagctgtca gaataaaatt tcttttcttt gatttaaaag cttgcaaaaa agagggagaa
    10801 tgagtattat taaaaatgta ggttagcact cgaaggttaa gagtgctaac agattaaaaa
    10861 aagagttatc ggagggattt atcgtgttaa aaccattagg tgatcgcgtc attatcgaag
    10921 tcgcaaaaga agaagaaaaa actgtcggag gaatcgtttt agcttctgct gctaaagaga
    10981 aaccccaaac tgggacagtt gttgcagtcg gtgaaggccg tttgttagaa aacggtgaaa
    11041 aagttcctgc cgctgtaaaa gcaggcgacc aagttatgtt cgaaaaatac gccggtacag
    11101 aagtaaaata cgaaggaaaa gaatatctga tcgtcgcagg aaaagatatc atggcgattg
    11161 ttgagtaaat ttggaagata aaaaaataat caaaaataat caatgaggtg aatcaatcat
    11221 ggcaaaagaa ttgaaatttg cagaagacgc acgcgcagct atgttacgcg gggtagataa
    11281 attagcagat acagtgaaag taacattagg acctaaaggc cgaaatgtcg tactagaaaa
    11341 atcttacggc tctccattga tcacaaatga tggtgtgacg atcgcaaaag aaatcgaatt
    11401 agaagatcat tttgaaaaca tgggtgcaaa acttgtatca gaagtagcat caaaaacaaa
    11461 tgatattgca ggagacggta caacgacagc aactgttctg acacaagcaa tcgttcgcga
    11521 aggtttaaaa aacgtaactg caggtgctaa tcctctaggc attcgtcgcg ggatcgaatt
    11581 ggcaacaaaa gcagcagtcg aagaattgca taatatctca actgttgttg attcaaaaga
    11641 agccattgca caagtagcag ctgtttcttc gggttctgat aaagttggac atttgattgc
    11701 tgatgcaatg gaaaaagttg gaaacgacgg tgtcatcaca atcgaagaat caaaagggat
    11761 cgaaacagaa ctagatgttg tagaaggtat gcagtttgat cgtggttact tatcacaata
    11821 tatggtaaca gataacgaca aaatggaagc tgttctagaa aacccatata tcttgatcac
    11881 agacaaaaaa atctctaaca tccaagatat tctgccattg ttagaacaaa tcttgcaaca
    11941 atcacgtcca ttgttgatta ttgctgacga tgtagatggt gaagcattgc caactcttgt
    12001 attgaacaaa atccgtggaa cattcaacgt agtagctgta aaagctcctg gtttcggcga
    12061 tcgtcgtaaa gcaatgcttg aagatatcgc tattttgaca ggtggtacag taattactga
    12121 cgatctaggt ttagaattga aagatgtaac aatcgaaaac ttaggaaatg cttctaaagt
    12181 tgttgtagat aaagacaata caacgatcgt agaaggttct ggagaaaaag aagcaatcga
    12241 agctcgtgtg caattgatca aaaaccaaat tgctgaaaca acttctgatt ttgaccgcga
    12301 aaaattacaa gaacgcttag ctaaattagc aggtggtgta gctgttgtta aagttggtgc
    12361 agctactgaa acagaattga aagaattaaa attacgcatt gaagatgcat tgaatgctac
    12421 acgtgccgca gtagaagaag gaatggtctc aggtggtggt acagctctag tgaacgttat
    12481 cagcaaagtt tctgctgtag aagcagaagg agacgtggca acaggtatca agatcgttgt
    12541 tcgtgcttta gaagaaccaa ttcgtcaaat cgctgaaaac gctggttatg aaggatcagt
    12601 gattgttgat aaattgaaga acgtggaact aggcactggt ttcaatgctg ctactggaga
    12661 atgggtaaac atggttgaag caggtatcgt agacccaact aaagtaacac gttctgcctt
    12721 gcaaaatgcc gcttccgttt ctgccttgtt attaacgaca gaagcagtcg ttgcagataa
    12781 accagaacca gcagcgccag cagctcctgc aatggatcca tcaatgatgg gcggtatgat
    12841 gtaagaaaaa gcaacttgct tatctacaac tgatcattag tgaaagacag ataaaaagag
    12901 gctggaacag aagcgtttaa ctccaagaaa taagaagaaa ttcacggaaa ttgcttttca
    12961 aatttttgtg aatttcagct tatttccgaa ggagttgctt ctgcttccgc tgtttatacg
    13021 tgtttagagc gtgagacaaa agtgttcttt acttttgtcc cacgctcttt tttgtgtgta
    13081 attataatga aaatagttat ttttaatagt tataatattt tatctattaa aagtaaatat
    13141 ccattca
//
LOCUS       DACKAC010000074        13074 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.075, whole genome shotgun sequence.
ACCESSION   DACKAC010000074 DACKAC010000000
VERSION     DACKAC010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.075"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(78..2198)
                     /locus_tag="I0B46_002292"
     CDS             complement(78..2198)
                     /locus_tag="I0B46_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAR2084551.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQLEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHKG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2428..3606
                     /locus_tag="I0B46_002293"
     CDS             2428..3606
                     /locus_tag="I0B46_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAR2084552.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3622..4380
                     /gene="aroD"
                     /locus_tag="I0B46_002294"
     CDS             3622..4380
                     /gene="aroD"
                     /locus_tag="I0B46_002294"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAR2084553.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4403..4888
                     /gene="ybaK"
                     /locus_tag="I0B46_002295"
     CDS             4403..4888
                     /gene="ybaK"
                     /locus_tag="I0B46_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAR2084554.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSHIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4959..6212)
                     /locus_tag="I0B46_002296"
     CDS             complement(4959..6212)
                     /locus_tag="I0B46_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAR2084555.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6328..7677
                     /gene="gdhA"
                     /locus_tag="I0B46_002297"
     CDS             6328..7677
                     /gene="gdhA"
                     /locus_tag="I0B46_002297"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAR2084556.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNAQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7789..9135
                     /locus_tag="I0B46_002298"
     CDS             7789..9135
                     /locus_tag="I0B46_002298"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAR2084557.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9443..9829)
                     /locus_tag="I0B46_002299"
     CDS             complement(9443..9829)
                     /locus_tag="I0B46_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAR2084558.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYTEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            10708..10920
                     /locus_tag="I0B46_002300"
     CDS             10708..10920
                     /locus_tag="I0B46_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084559.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPTELVS"
     gene            complement(11198..12967)
                     /locus_tag="I0B46_002301"
                     /pseudo
     CDS             complement(11198..12967)
                     /locus_tag="I0B46_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879453.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
BASE COUNT         4288 a         2350 c         2576 g         3860 t
ORIGIN      
        1 tattctaaga tttctcttta ccaacatcat ttgacaaaga gcctaatttt gtcgctgctc
       61 cttttaaatt tattttatca ttcggaagaa cttgtggtcg ttccgtgaag ttcttggtac
      121 gtttgcgttt tataaagatc aacagttgat tgtcccttgt gttcactaaa gacactagtt
      181 gacttgtcac caagagattt ttcagcttct tttaatttct ctaagccatt cttgtagtca
      241 taatcactgg tatcaattgg gtctaatcca ctattcgtat agaaccgaag aagatcacca
      301 ttattgattt ggtcagaaac agctaactgt ttatcaactt tttctttcag tgaatcaatt
      361 tcattctgta cttcctcagt aggttcagta atagcttcac cagtttcgtt actatagatc
      421 gtaccaccgt agtaagtata ttttggtgta acaaaatcac catcacggaa agaaacaagc
      481 tgttcatgat ctttcgagaa tagatcttgt cctaactgaa tataattttt tgtatctaca
      541 cctagtaagt gaagtaatgt aggcaaggca tcaacttgtc cgccgtaagt gtgattgatt
      601 ccgccctgtg tttgtccagg gatatggatc atataaggga cacgctgcat ttgtgcattg
      661 tcgtagtttg tccaagtaga acttgttttt ccaactaatt cagctagatt tttatttctt
      721 gaattcgaga caccgtaatg gtcaccgtaa aggacgatga ctgaattatc atacaagccg
      781 ctttctttca aataattgaa gaattcttgt acggcggtat ctaaataatt ggctgtcgca
      841 aagtaaccgt tgatcgtttc atcatttgta ttggctaacg ggaatcctgc ttcgtcattg
      901 gtgaattttg aataaggata atggttagat acagcaatga attttgaata aaatggttgt
      961 tgcagacgtt ccaagtattg tacggattgt tggaaaaatg gtttatcatg caacccgtat
     1021 tggaacgaat tgtcatcatt aacatcataa taagaagaat caaagaaata atcatatcct
     1081 aaattcttgt atgtctcgtt tcgattccag aatgtaccag cgttcccatg gaataccgca
     1141 ctagtgtaac cttgtgtttg acttaaaata tcaggtgcag cttggaaagt attttttccg
     1201 cctaattgtg tgaacaagga gccttgattc aagccaaaca atgaattttc catcagcgtt
     1261 tccgcatcgc ttgtttttcc tgcttttact tgatggaaaa agttgtcaaa actgaacgtg
     1321 ctgttgctgt ggtaaagact attgatgaaa ggtgtaactt caagctggag accattctca
     1381 tctgtcaatt tataatcgat caggaattgc tggaagcttt ctaaatggat ataaatcaca
     1441 ttttttcctt tagcgagccc gaacaaatca tcatttggtg ctgcataatg ttcttttaca
     1501 taattttcta cttctttcat atcattggga cttgcttctg cccgcacttg gtttgtctgg
     1561 taagtttgaa caccgtcata aaccatgaaa gcattcaagc caagatattt gaccaaataa
     1621 tcacgagaga atgttcgagt cagcaattct ggacgatcag cttccgccat aaaaagattt
     1681 cctgagaaaa tcataactgc taatgtagag atcgcaaaag ccattcttgc tcggatcggg
     1741 cgagggtcca acttgatttt ttttgcaatc agcagaacag tcaagataat aaaatcgatc
     1801 caatagatca catcatatgg gcgaaacagt cgaatggcac tttctcccag accactcgct
     1861 actttcccag cacctaaaat cgtgttgatc gtaatgaagt ccgtgaattc tcgataataa
     1921 gtgacattcg aaaaaacaag caatgtcaaa agaaaataaa tgaccatcat cgtgatataa
     1981 gcaactctag ggcgtctgat ataaagagca attccaagta ataacaatgt agttgcaata
     2041 gggttgataa acaagataaa gtattgaatg ccactttcga ttccgagatg gaaatcaact
     2101 aaataggcaa aaatcgtttt caaccaaagt aaaacaacta gtaaagaaaa aaatccgagc
     2161 ctcttattca aaaaggcagg tgtttttata catttcaaga taatcttcct ttctgcactt
     2221 cttaatcaca gaagtgtcat gaaaccatgt tacacagctc acttattctc atttatttta
     2281 ctagtatgac aatatagtgt caattgaaac cagaagttta tgctgtttca ttaaggtttt
     2341 ctttaagctt catgaaaaaa tgagcagtaa gacaagtcct atgttttttg ttatacttgg
     2401 gaagagaaag ctaaagcagg tgaaggaatg aaagtaaaag taaaaaaaag agcaagcaat
     2461 cgattgaaaa aaggatatcc actgatccaa aaagaagacc ttgctcatcc ccaaaaagaa
     2521 accacgcaat gggtggaatt agttgattcg caaaaccaat ttatcgcaaa aggatatcta
     2581 ggaaagcaga ataagggaat cggttgggta ttgagccagc agaacatccc atttgatcaa
     2641 tctttttttg aagaattttt tgtgaagtca aaagaaaaaa gaactttctt tttttcagat
     2701 gattctacta ccgcttttcg tatattcaat ggagaaggag acgggatcgg cggtctgatt
     2761 gttgattatt atgcaggtta tgctgttttt tcttggtata acgaaacgtt atatgcacat
     2821 cgtgaattat tgattcaagc ttttcgtgaa atctacccgg aaatcgtagg aggctatgag
     2881 aaaattcgat ttgaatcagt aaatctgcct gagagccagt tcatttacgg aaaagaggca
     2941 ccggaaccct tgatcgtaaa agaaaacgga gtaaaatttg ccgtttattt aaatgaagga
     3001 ttaatgactg ggatcttttt agaccaaaaa gaagtacggg gatcattagc agaaggtttc
     3061 gcttctggga aaacagtttt gaacatgttt agttataccg gcgccttttc tgttgctgcc
     3121 gcaatgggag gggcaatcga aacaacaagt gtcgatctag caaaacgcag tttaaaaaaa
     3181 acacaggaac agtttgaagt gaatggtttg gatgtcgatc agcaaaaaat cattgttatg
     3241 gacgtattcg agtatttcaa atacgccaaa agaaaacaat ttttatatga catgattgta
     3301 ttggatccac caagttttgc cagaaataag aaaaaagtct ttcgcgtagc aaaagattat
     3361 ggggaactgg tcaaggatag tctggatatt ttagcagaag acgggatact tattgcgtct
     3421 acaaatgctg cgaatgtctc gatagaacaa ttccaaacaa tgattgaaga ggaattccat
     3481 gccaaaaatg tttcttttga acaacttgct ttatatcgtc tgccaaaaga tttccagacg
     3541 acggatacct ttttagaagg caattatctg aaagtgtttg tttatcaaat taaaaataac
     3601 cattagaagg agcgatgcag aatggtttgg gtaaagggat tgaatatagg aagcggtaga
     3661 ccaaagattt gtgcgcctct tatcggggcc gtccgagagg aagtattacg tgaagcagtt
     3721 cttgcacgtg aagcaggggc cgatttagtc gaatggcgag tcgatcatta tcgtgaagtt
     3781 ttttcttttg atgaagtagc agaaacattg gttcttatcc atgagatgct tgatggactg
     3841 ccgttattat ttacgtttcg taccttagct gaaggcggag aacaagacat atcgatccgt
     3901 cagtatcgcg acctttatga acatgtgata cacacaggat tagtagatat ggtcgatatc
     3961 gagttgttca aagtagaaag tctaggaaaa agactgatag aggaaatcaa gtctttaaac
     4021 attcctttaa ttatcagtag tcatgatttt aaagaaacac cagcagaccc tgtcttgctt
     4081 taccgattga atgttatgga acattttgga gccgatatcg gaaaactggc agtaactccc
     4141 aataatgaac gagatgtttt gcggttgatg gagttgacta gacgggcaaa tgcttttgtc
     4201 tccatgccta ttattacgat gtccatggga aatcttggta aaatttctcg tctggctggc
     4261 ggaacgacgg gttccgttat gacatttggt tccttaagca gcgattctac ttctgctcca
     4321 ggacaattac cggtcaaagc attgaagcaa attattcaat tattaaatga accgttgtag
     4381 aacaagtttg aggtgatatt ttttggcaaa gaaaaaaata gtaaaaacga atgcaatccg
     4441 tatggtcgaa caaaaaaaga ttccctatac agagcacgaa tatgagtggg acgaaagcca
     4501 cctaagtgcg tcatcggtcg ctgaacagct tccagaaagc caatctcata tatttaaaac
     4561 actggttgct gtgggcaatg tgacggggcc tcttgttgct gtgataccgg gagaagccga
     4621 attgaatcta aaaaaattag caaaagtttc agggaataaa aaagtagaaa tgctccattt
     4681 aaaagatttg gaagcaacga ccggatacat acgtggtggc tgctcgccta taggtatgaa
     4741 aaaactattt cctacttatt tagatcaaat tgcagaaagc tatgaacaaa tcattgtctc
     4801 ggctggcaga agagggctac agatggagtt agcacctcaa gatatctgtg cattaacgag
     4861 cggacaattt gcagatataa aacaataaat catacgtata aaagaaagaa ggaggccggg
     4921 atgttgtttg tccagtctcc ttctttcttt tttatacatt atgctctttg cccggcattc
     4981 attcctgcta catgccctgt agcgaaagcc gctgtgatat taaagccgcc agtataacca
     5041 ttgacatcca gtaattctcc cgcaaaatac agaccatcaa tgattttact ctccaatgtt
     5101 ttaggataaa tttcctttag agaaacccct ccgccagtta caaatgattt ctcaatagga
     5161 aaagttttgt tgattgatag cttaaactct ttgcatagtt gaacaaaatc ttgaatttgt
     5221 ttttgagagg cttgttgtcc tgtcagatga tccatttcaa gccgctcaag aaagaaaact
     5281 aacaatcgct caggcaatat accatgccaa gcattgacca agttcttttt ggttgcttta
     5341 cttttttctg tcaatatatg aataagctct tgtttggttt gtgtagggaa acaatccaga
     5401 gaaagtgtca ctggttctcc cgttttctct aattctttat tgacaaaact tgaacaacgc
     5461 agtgcagccg gaccagaaat accgaaatgg gtgaaaagta aatccatcgt atgttcagtc
     5521 aatacacgac cttttttatt taaaacgcgt aaagtgatgt cttgtaaaga aagcccttga
     5581 agtgttttct cctgaatata cggttcatcc gatatcaagg gggactctgt cgcataaaga
     5641 ggcgtcaccg tgtgacccac tcgcttcacg atcttgtacc catctccagt agaaccagta
     5701 gatggataag tccgtccacc agttgttaag ataatggcag gagcatggaa ctcttcgaat
     5761 tccgtgcgta taccgtaaat tttatgttct ttgtggatca atttctctac tcgagtagag
     5821 aatagaagcg tcacatctag ttctttcaga cgattgagca atgcttcgat aatcgttttt
     5881 gatttatttg ttacaggaaa catccgtcca tgatcctctt cttttaaatg tacgccttgt
     5941 gattcaaaga agttcatgat atcgaaatta ttccattgcg caaacgtact atataaaaac
     6001 tttccatttc caggaatatg tgcaatcaaa tcatcaacag gacgattgtt cgtaacattg
     6061 caccgaccac cacctgtcat cagtagtttt tttcctgctt ttttgttttt ttcgatcaag
     6121 agtacgcgtg cgccttgttc agctgcactg atcgctgcca tcatcccgct tgttccagcg
     6181 ccgactacga tcacatcata cagtttttcc attccctcac ctcaaggaaa gtgtagcata
     6241 tctgaagaaa gaaaacagaa aaaatgtttt gagccaaaag agaaaatgag ctaaaatgag
     6301 ggaagataag caaaggagcg tggaacgatg acaaacgcaa cagaatatgt tcaagcaatt
     6361 caagaaaagc tacatcaaaa ggatcaaggg caagaagaat ttttacaagc aatcgatgaa
     6421 ttcatgccca cggttatgtc ttttttggac actcatcccg aatatatcga aaagaatatc
     6481 ttagaacttt taacggaacc agaaagagtt attcaatttc gagtgccttg gcaagatgat
     6541 gaaggaaact ggcgagtaaa tagagggtac cgtatccaat acaactcagc tatcggccct
     6601 tataaaggtg gattgcgctt ccatcctagt gtcaatctaa gtatcttgaa atttttggct
     6661 tttgaacaga ttttcaagaa tagtttaacc ggtctgccaa tcggtggagg aaaaggcgga
     6721 agtgacttcg atcctaaagg aaaatctgat aatgagatca tgcgtttttg tcaaagtttc
     6781 atgctggaat tgtcaaaaca tatcggccca tcgattgatg ttcctgcggg agatatcggt
     6841 gtaggagcta gagaaatcgg ctatttgttc ggcgaatata aacgattgaa aaaatatgat
     6901 actggtgtgt tgacaggtaa acctttggat ttttggggaa gtaagatacg tacggaagct
     6961 actggttatg gattagtgta ttatgtcaaa catctattga acgaagaaaa agattcattt
     7021 aatcaaaaaa cagtttttgt ttccggaagc gggaatgtag ccatttatgc aatcgaaaaa
     7081 gtgcaagagc taggcgggaa agttgttaca tgttcagatt ctagcgggta catctatgat
     7141 ccagaaggaa tagatgtgaa actattgaaa gaagtcaaag aaatcaatag acaacgtttg
     7201 acggaatacg caaaccaacg tccttctgct atttatcatg ctggagaatc tgtttggaca
     7261 ttgaaagaaa aagcggatat cgctttgcca tgtgctactc aaaatgaaat agacgaaagc
     7321 ttggccgaaa tcttagtaga aaatgggatc aaaatcgtag cagaaggcgc taatatgcca
     7381 tgtacagtaa aagctgtttc tgttcttcat gaagcaggcg tgtggtattg tccaggaaaa
     7441 gctgctaatg ctggaggagt agcagtatct gctttagaaa tgagtcagaa cgcccagcga
     7501 ttgacttggg aaagagagca agtggatgag cagctggatc aaatcatgga aacgatttac
     7561 catacgtgtc gccaaacagc agccgagtac ggcgacgaaa aaaatctgct tcttggagca
     7621 aatgttgctg gatttgaaaa agttgccaaa gcaatggcag cagaaggttt agtttgaaaa
     7681 atgaaacttt cttggaaata ttgtcagcat tttgtttatt tgcttgaaaa ataataaaaa
     7741 aaaacgtaaa gtattacctg tagagtaaac ctataaggag gagaacaaat ggcacacatt
     7801 cattttgact attcaaaagt agcaccgttt gtcaacgaac aagagttagg gtacatgcaa
     7861 agccaagtaa cagcagccca caacgaatta cgggaaggta ctggcgcagg aagcgacttc
     7921 cgcggctgga tcgatttacc aacgaactat gacaaagaag aatttgcacg tatcaaagaa
     7981 gcagctaaaa aaatccaatc tgattctgaa gtattagttg ttatcggtat cggtggttct
     8041 tatttaggcg ctcgtgcagc gatcgacttc ttgaatcaca cattcttcaa tttattagat
     8101 agcaacacaa gaaaagcacc acaaatcttc tttgccggaa attcaatcag ctcaacatat
     8161 attgctgact tgatcgaagt aatcggtgat cgcgactttt cagtaaacgt tatttcaaaa
     8221 tctggaacaa ctactgaacc agcgattgcg ttccgtgtat tcaaagagct tcttgttaag
     8281 aaatatggac aagaagaagc aaacaaacgt atctacgcta caacagataa agcaaaaggt
     8341 gcagttaaag tagaagcaga tgcagaaggt tgggaaactt tcgtgattcc tgatgacatc
     8401 ggcggacgtt tctctgtatt aactgctgtt ggtttgttac caatcgctgt tagtggtgca
     8461 gatatcgatg cattgatgca aggtgccgct gatgcaagca aagcatactc aagcgacaaa
     8521 ctagaagaaa acgaagctta ccaatacgca gcaatgcgca acattcttta ccgtaaaggc
     8581 aaagtaactg agttgttgat caactatgaa ccaggaatgc aatacttctc agaatggtgg
     8641 aaacaattgt atggtgaatc tgaaggaaaa gaccaaaaag gcatctatcc ttcaagcgca
     8701 aacttctcaa ctgatttgca ctctttagga caattcatcc aagaaggacg tcgcaatatt
     8761 tttgaaacag ttgtgaaggt tgaaaaacct cgtaaatcaa tcacaattcc tgaacaagca
     8821 gaagatttag acggtctagg ctacttgcaa ggaaaagaag tggacttcgt aaatacaaaa
     8881 gcatttgaag gaacattgct tgctcataca gacggtgacg tgcctaactt gctagtcaaa
     8941 attccagaaa tggatgctta tacattgggt tatacaatgt acttctttga aattgctgta
     9001 ggtatttcag gttacttgaa tggaatcaac ccattcgacc aaccaggtgt agaagcttac
     9061 aagaaaaaca tgttcgcact tctaggcaaa ccgggctttg aagatcttgc taaagaattg
     9121 aacgaacgcc tgtaagataa gggtttttcg ctagatttaa ataatcagtg atagaaacat
     9181 gttcgcttta aaatgaaaac tggtacccat ttaggtactc aaacatactg catcgtttat
     9241 tcagaataag gggctgtgac aagacgtttg tcacagtccc ttgtttgtat agaaatcgat
     9301 tgattgcctt gctctttagt ttgttttagg actttaagaa cagtgaggct gacttcgaag
     9361 gttaattctg tttttttgtt ttctagaatc agtaagcaaa aaagcactca cttcgagtgc
     9421 ttttttgcga tatattctta gcttaagata ggcctaattt ttcttttaca gatgagggta
     9481 ccttatcgct ggtaattact tttttctcta tgatccgctt ttcgcttgct ttcaactgca
     9541 agatcgttcc ttcggtgtaa gcagaaccat tggcagagtc accagaagta atgacatcga
     9601 aatctaactg tcttttttca ccgttttcag cataagcatc tacgttgtat ttaaaacctc
     9661 ttccaattac ttccccatta tctgctttga tatcagtttc tgcaggcatt ggagctttga
     9721 ctaccgcata ataatcttta cctacataag tagactgata gtagtcccaa cctttccatc
     9781 ctataaaagc aatcaatatg ataacgatca accctaaaat ttttttcatg aaagatctcc
     9841 ttttttattt ttattatatt aaatattata cagataataa cggaatatta tcatcgaaac
     9901 agctaacaaa attgtcataa aaaacgagat ggttgatatt agaacttgtg tttttgttta
     9961 ggtctagaga taaagagaga caatttccaa atatatgtaa tgaatacaaa aagacgtttg
    10021 tcgcggatta ccgttacaaa cgtcttttat tgattttccg agatctcatc agtcgccatt
    10081 tggaatggtt cactccttat ctagattcgt ttaatgctcg gactgcttgt tcgattgtaa
    10141 taccaaacgc aacgttattt tcgttttgtg catcaatcgc cataaaacgg ttagaagttg
    10201 tatcaaaagc tacgcggtat tgcatatttg tttttgtaaa cgtcataaaa ttcatctcct
    10261 ttgtaatgta atttagttct tgaaagtttc ctttaaactt tcgaacttat accaagttta
    10321 gcatcgtacg ttttttgatg caattaaaaa gcatttatca ttttccttat ttattttttc
    10381 ttaaatggtt tagctatttt cttttaatcc gataagtagc ccaatcatca aggttacaaa
    10441 tactattgct tgaatataaa tcatatgctc acctctttat aaataactta gcaatataaa
    10501 gaaaaaaacg cacgaacaac ataggaatat aaacaaacgt ttttgtcaca acgtctaaga
    10561 tcgtgttaaa aaatagttat ctaaaaagca gaaagatact caaaataaaa aataaaaaaa
    10621 tgaataaaaa cgttttcaaa tacaaggtaa tttgttatta taaaataaag aaaaaacaaa
    10681 ataaaaataa agaaaagagg gaatgaaatg gtaaaagcag aagtaggaag atcttatata
    10741 tgcaaaccga tcggagtatc tcatgaacta atcggctgta ttgaacgtac atatatcaac
    10801 acggctgtcg tgtatgttga aagttatgac caacgagact atcaacttat caatgaaagc
    10861 aagtatcgca tgctcgtaaa attctgtgat attagcgcac ctactgaatt ggtttcttaa
    10921 acagtatttt tgagaattcg caagaactcc cttataatat aaataaagac agaagggagc
    10981 gagaagatat gccaaaaaga aaaagttcag ggaaaatggg aattattctt gcagtgatct
    11041 ctgcaattcc tgctatttta gctattgttc gtgcgatgaa ggaatcaaga gaaagttaag
    11101 cacacattat gagcgtcaaa caaaaaacgc atatacctgc ggtggcaggt tatgcgtttt
    11161 ttgttgtata aagacttttt tcattaacga tttttgatta gccgactccc aaaacaaaat
    11221 ttagcagttt atctgaataa gtgatttctt catctgtttg tttcacactt tcatttagta
    11281 gtcccatctc atcggttatg ataccatcca caccataaaa catcatgcga ctcatggcat
    11341 ctgaatcgtt gattgtccat acatatactt cttttccgtc ttggtgggca gaattaatga
    11401 agttacggtt gatcgtacta tattccattg tcagaaaatc agcaggcgtc acagggggcc
    11461 caacaatatt gaaaggtaag atgtagccca cataaaagtt tggttctgct tcttttaaat
    11521 ctgaaacggt ttggtacgtc aaactttgca aaatatgatg gttctctaag ataactttgc
    11581 ggtatttatg gacaaatcgt tcgacaaggt catccgaatc ttttttagag gtcttgatct
    11641 caataagtaa tttttggttt agcttatgtg ctttttccaa ataagaatca aaagaacaaa
    11701 caggggcttg cactccgttt tcagtaactg taagatttgt caattctttc aatgtaagat
    11761 cctgtggtgt tccatttacc cctgttaagt gacgaagatt tgcatcatgc atcactacga
    11821 aatgaccatc ttttgtttcc tgaacatcca tctctacata atcaggatga tagtctctgc
    11881 ttgttttttc aagtgcttct aaagtatttt gcgccccatt ttgggtactc acacctctgt
    11941 gagagacagt cactggtttt gtctgaacag ctgaagtcag ataatccata ttgtaaagac
    12001 agacgccaat accgaaaaaa actgcaaaaa gagtcaaacc agtgtttttc agcgcagaga
    12061 aacgcaagtt gggggcttgt gggataaacc acttagggat ctcaggcaag aagccttcgt
    12121 catccatgaa atcaacaatg atataaaaaa tccctacggt agacatcacg atattgaata
    12181 ataaaatccc ttgtaaaagc gtcatagcaa acacagcgct gatcaatgca tagtctggga
    12241 aaaattgttc aaccatactt tgtgccaaaa taacaacgat atatcctaaa gaagaaagga
    12301 gtaaaatcgt accgccaatc acaataaatt ggcccgtgat ggccaaaaga cgcgatttgg
    12361 tcaatgacca gctttcccga atggctgctc gaaaagaacg atctcgtaga atcatttccg
    12421 gtaaagcaaa aatcagtcga atcccaatat agccaaggaa aatataaacg agtaaaaagg
    12481 aactgacgat gatccaacga ttagcaaaaa taaaatccat aatgaaagcg gggattttta
    12541 tttttgacag tagatcagaa ttaaaactta atccgctgat cggtagaatc aataaaaaat
    12601 aagcaaggaa aaacaaaaaa gtaatgggtc tcactttctt cagctgaagt atcgtcaagt
    12661 gtaagagttg cttcaaagag attggttctt tctttttaat aaaaaaaaca ctcatcaaaa
    12721 gaaaagtaaa ttcgaaataa actaacagta aaatcgtcat caaaactacg attaacgaga
    12781 gtagaacccc aggatgttga gaaaaaattg aaggaatatt gtcataagac aaatagtcaa
    12841 ttgcccctcg gtgtaaaata aaacgggtcg tactgctcaa taagggtaaa caaataaata
    12901 aaataaagcc atgcatcagc aagacatcac ggaaataagc ttgtgttcct tttaaaaagt
    12961 cccaagcata cgtttcaaat aattcatcct atcacctgct cttcacattg tagcgcaaat
    13021 ctaatgaaaa aggaaagggt gcagtgtgtt ttgcagaccc tttcccagat ttaa
//
LOCUS       DACKAC010000075        13056 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.076, whole genome shotgun sequence.
ACCESSION   DACKAC010000075 DACKAC010000000
VERSION     DACKAC010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.076"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            157..1080
                     /locus_tag="I0B46_002302"
     CDS             157..1080
                     /locus_tag="I0B46_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAR2084560.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            1098..2339
                     /locus_tag="I0B46_002303"
     CDS             1098..2339
                     /locus_tag="I0B46_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAR2084561.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            2473..3819
                     /gene="gor"
                     /locus_tag="I0B46_002304"
     CDS             2473..3819
                     /gene="gor"
                     /locus_tag="I0B46_002304"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAR2084562.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(4633..5586)
                     /gene="menA"
                     /locus_tag="I0B46_002305"
     CDS             complement(4633..5586)
                     /gene="menA"
                     /locus_tag="I0B46_002305"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAR2084563.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(5599..6702)
                     /locus_tag="I0B46_002306"
     CDS             complement(5599..6702)
                     /locus_tag="I0B46_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAR2084564.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(6792..7727)
                     /locus_tag="I0B46_002307"
     CDS             complement(6792..7727)
                     /locus_tag="I0B46_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAR2084565.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            8029..9963
                     /locus_tag="I0B46_002308"
     CDS             8029..9963
                     /locus_tag="I0B46_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAR2084566.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            10420..10836
                     /locus_tag="I0B46_002309"
     CDS             10420..10836
                     /locus_tag="I0B46_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAR2084567.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            10850..11416
                     /locus_tag="I0B46_002310"
     CDS             10850..11416
                     /locus_tag="I0B46_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAR2084568.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            11439..12425
                     /locus_tag="I0B46_002311"
     CDS             11439..12425
                     /locus_tag="I0B46_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAR2084569.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(12580..>13056)
                     /gene="scm"
                     /locus_tag="I0B46_002312"
     CDS             complement(12580..>13056)
                     /gene="scm"
                     /locus_tag="I0B46_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAR2084570.1"
                     /translation="STDTSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSST
                     TTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNR
                     TNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT         4065 a         2369 c         2695 g         3927 t
ORIGIN      
        1 ttttgtcaca atctcttttt atatttggac agaatatatc gaactttatt ttgatttttt
       61 gtgaaataat tttcaaaaat ccgactttaa atagaagaag tattggaaag tttttagagc
      121 tatgctacta ttatgaagtt gtaggaggag aaaattatga tcgaactcat aaccacagta
      181 gaaactattg aacaagcaga acagttgctt gctgcaggtg cagatacact atatttcggt
      241 gaagagcaat ttggattgcg tctgcctgct tcatttacac gagaagaaca gcgacaattg
      301 gtagaattag cacacaagta tggtaaaaaa gcgaatattg ctgtaaatgg gatcattcat
      361 cctgaaaaaa tgaaaaatgt acctgaatat ttggcttttt taaaagaaat aaacgtagat
      421 gtgatcacag ttggtgatac aggtattatt tatacgatga aaaaaaatcc tgaattgcgt
      481 ataccgtata tttatgatgc tgaaacatta gtaacgagcg cacgtcaaat caatttctgg
      541 gcgaaaaaag gcgcagttgg agcagtactt gctcgtgaag ttccttttga agagatgaag
      601 gcgatggaag aaaaactcga tattcctgta gaaacccttg tgtatggtgc tacatgcatc
      661 catcaatcca aacgtccact gctacagaat tactataact atacgaaaca agacgaacaa
      721 aaagatcgtg aaagaggtct ttttatctcg gaacctaaaa aagaagagac acattattct
      781 atttatgaag atagtcatgg cacacatatt tttgccagca atgatctgaa tctctcgaat
      841 gaattgaatg aactttatca gcatcattac tgtacatgga aattggacgg attatttact
      901 ccaggcgaaa aattcgtaaa catcactcga ctattcgccg aagcaaaaaa acaaatttta
      961 gaaggtagct gggatcaaga aaaagcggaa aaaatgacta gcgaaatcag taaattgcat
     1021 cctgaaaacc gtgaattaga tacaggattc ttttatattg atccttctgc tatcaaataa
     1081 ggagtatgat gaaagaaatg acagtaaaaa gaaaattgaa acgaccagaa atcctagcgc
     1141 cagcaggaac actggaaaag ttaaaaaccg cgatccatta tggagcagat gcagtatata
     1201 ttggcgggaa tgcatatggt ttaagaagcc gtgcaggtaa ttttacttat gaagaaatgg
     1261 ctgaaggagt tgcttttgca aaagaacatg atgcgaaggt atacgttgct gctaatatgg
     1321 tcacccatga aggaaatgaa gatggagcag gggaattttt ccgcagtttg cgtgatgtcg
     1381 gtatttctgc agtgattgtt tctgatccag ctttgatcga aatttgtgcg acagaagccc
     1441 caggtctgcc gatccattta tccactcaag cttcagcaac taattttgag acattggaat
     1501 tctggaaaga agaagggttg gaacgtgtcg ttttagctcg tgaagtttca atggaggaag
     1561 tggcagagat tcgtaaaaat acggataccg aaatcgaagc atttatccat ggtgccatgt
     1621 gtatctctta ttctggtcga tgcacattgt ccaaccatat gtcgatgcgg gatgcaaacc
     1681 gtggcggatg ttctcaatct tgtcgctgga agtatggatt gttcgatatg ccttttggtc
     1741 aagaacgaaa cagtttaact gatacaggcg aagtggaaga agaattctcg atgagtgcag
     1801 tggatatgtc aatgatcgaa catattccag aattgattga aaacggcgta gacagcttca
     1861 agatcgaagg aagaatgaaa tcgatccatt atgtatctac ggtgtcaaat gtctataaag
     1921 cggctgtaga cagttatatg gaagatcctg aaaactatgt ctgcaagcaa gaatggatcg
     1981 acgagttatg gaaagtagca caacgagaat tagctacagg tttttactac aatacaccaa
     2041 ctgaaaacga acaattattc ggtgaacgtc gtaaaatccc agtatataaa tttgtcggtg
     2101 aagtattatc ttatgatgag gcaacacaga ttgctacgat acgtcagaga aacttgtttt
     2161 ctgtaggaga tgaaatcgaa ttttatggtc caggattcag tcatttcagt caaaccgttg
     2221 aagttatgca taatgaagaa ggcgaatcca ttgaccgagc accaaatcca atgatgattt
     2281 taactatgcc agtcgcacag ccggtgaaac ctggagatat gatccgaaaa cgtaaataag
     2341 gaaaaagctg atatatccct gttttaagca tcttcgcata caaaattcaa gaaaagtgtt
     2401 acacttgttt tctgtagaaa aaggatcgtt aaataaaggt tttgattgat ctcttcaata
     2461 aggaggaaca aaatgcaaat atatgattac atcgtaattg gtggcggctc tggcggtatt
     2521 gcttcagcaa acagagcagg gatgcatggc gcaaaagttt tgctgattga agcgaatgaa
     2581 ttgggcggaa catgtgtcaa cgtcggttgt gtacctaaaa aagttatgtg gcaagcaagt
     2641 acgattttgg aaacaattga acgagatgcc tcaagttacg gtatccaagc agatttaaaa
     2701 aatgttgatt tcgccggtct agtagaaaga cgagaaaaat atattgattt tctacacggt
     2761 gcttatcaaa gaggattaga tagcaatcat gtcgaggcta taaaaggtta cgctactttt
     2821 atcgatagtc aaacgatcga agtaaacggt gaaacctatc gtgctcctaa aatcttgatc
     2881 gctactggag gccgtgcaag taaaatgacc attcctggtg gagaatacgc aattgattca
     2941 aatggatttt ttgaattaac agagttacct aagagagcaa tcgttttagg tgctggatac
     3001 attgcggctg aactttcagg tgtatttcgt ggtttaggta gtgaagtcat gtgggcatat
     3061 cgaaaagaac gaccacttag aacgtttgat aaaatgcttt cggacaattt gatccaaatg
     3121 taccaagaag ctggaataaa aacttacgca catcatattg ctaaagaaat tactaaaaac
     3181 aatgacgaat atacggtgct atttgaaaat ggtgaaacct taactggtga ttgtgtactt
     3241 tttgccggtg gacgtgtacc gaataccgaa aaattaggtc tggaaaatac aaatgtggaa
     3301 ttggacaaaa aagggtttat caaagtagat aaataccaaa atacaacgga tgaacatatt
     3361 tttgctgttg gtgatgtgat cggtaaatta gatctgactc ctgttgcaat cgcagctggt
     3421 agacgtttat cagaacgttt atttaatgga aagaaagatt cttatcttga ctataaactt
     3481 gttccgacag tcgtctttac gcatccacca attgcaacaa ttggtttgac agaagaagaa
     3541 gcattggaaa aatatggcga aaatgaattg aaagtttatc gttcacgctt taccccaatg
     3601 tactttgcat taaatgatta tcgccaaaaa tgcgaaatga aattgatctg tgtcggaaaa
     3661 gaagaaagaa tcgtcggctt acatgctatt ggtgttggtg tcgatgagat gctgcaaggt
     3721 tttgccgtgg ctatcaagat gggtgcaact aaagaagact ttgacaatac agtagccatc
     3781 catccaacag gagcggaaga attcgtaacg atgcgataat ttaatattaa cagttccaac
     3841 agctttttat tgaacaaacc atgtaaaacc agaaaatcag tatactgatt ttctggtttt
     3901 ttgtttttta gttatttatg taagattgct tcgataattc tcaaaacgaa ataagtaggg
     3961 aagttggaga ttttgattat cttcgtaact gacgaagagt aaatgaatcc atttggtatg
     4021 cactcttaag ttcaaaatat tggtatcgat gtttaaaaga aatagaaatg tatcgaagaa
     4081 cagatgttta aaaaagtgtg actttgtata aataaattag aagaagcaaa cgaggatgtt
     4141 aaaagaatac catatagtta ccaaaccaag aaagttctcg tcaagttcgc tggtacgaat
     4201 caaatcaagt gaaaaaaggg actgtgaaaa aaattgtgtc atagttagtc actttttccg
     4261 aacaaatagt gttcaatcag ctgtccttcg gtattagctc aaaaaacggc aaaatatgag
     4321 gaactatttt tccatttgtt tctctaatac tcaggacaaa acactgattt tacaacctct
     4381 gataaacggt gttcaaaagc cagtttctcg gtattaagcc aaatttatgc aaaaatgtgg
     4441 aaagccattt tcacaaaatt tttcttaata ctcgaaacta agcgactttt tcacaacctc
     4501 ctgataaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaaccaa acgaaaaata
     4561 taaggagcta tttttatttg gctgttctta ttacttgatg cagggcagtt ttttcacaac
     4621 ctcttcatgc tgttaaatgg cttgcataat gattgttagt aatagcccca atacataact
     4681 ggcattgaat aagaccatat ttttgatgga atagctgaaa ctgataggct ggggtaacgt
     4741 ttgcttgaat tgatccaaat gcttcttcac aattggcaag gtcaagaagg tgaccaagat
     4801 cggccattca tagataccgc tcaacaaccc cattaaaatc acgaaatagc aagcatacat
     4861 caatagctgg aataagactg ttcccgcagg acgtccgata taaaaaacta gtgtatagcg
     4921 atgattttca atatcacggt caagatcccg caaattatta gccaacatga tattggcaat
     4981 cgtaaacacc aagggtaagg aagctaatac gattgctgct atagcccaaa gatttcctac
     5041 caaatcaaat tgtccagatg aaaagttgac tgtcagatca tagacatctg aaccgaatgt
     5101 attgacataa acgaccatgg caaaaattcc aaggcccata gtcaccccac tgaaaatttc
     5161 ccctaaaggc attcgagata aaggaatcgg tccgaatgta tagaatactc caataaaaca
     5221 acagataaat cccatcaaaa gtaaaagcca acccgtattg atcgttagaa taacgccgat
     5281 aacaaaaaca aatgcaatca tagctaaaat catgtttcga atcaatacag gagatagatt
     5341 tctgcggcca atgacatttt cttcatactt atatacttca gaatgagcct tttcataatc
     5401 catgtaatta ttgatagcgg tagtcgctaa atcaaaaact agcattccaa taaaaaataa
     5461 aaaagtgttt agccaatgta tctcatgaaa aaaagcgata gagaataata cccctaccgc
     5521 aaaaggaaat acactagcca cctttgttcg tatttcaact acttctaaaa agatttttag
     5581 tgtcatggtt cgtactcctt attttagttc agaacggtca cccatcgtgt aatgggagtc
     5641 ctccgataat ttgaaggtgt ctttgattgg atcggtcaca tatactttat cgtcttttgt
     5701 cacgaagatc gcttccgtcc cgttattcaa ttcgctttcg acgtattcca gtcctttttt
     5761 cacacccatt gaaaagactg ctgtagacaa cccgtcacca tcaattgatt tatctgttat
     5821 gatcgtgaca cttgcgatat cattgtcgaa cggataaccc gtttttggat caaacaaatg
     5881 atggtatttt ttgccatcta cttctaaata acgctcgtaa attccagagg taactaatgt
     5941 tttgttccgt tctttaatag aacctaaaac agatccgcga gccatgttcg gatcttggat
     6001 gccgactgtc cagtcttggt tttcgcctct tgggctgtgg cctaagacat agacattacc
     6061 gcccaagtca acgatggcag tggtcactcc ttgttttttc aagactttca ctacttcgtc
     6121 cgtaatgtaa cctttggcga ttgcacctaa atcgatgatc attccttttt cttttagata
     6181 aacagttttt tctttgtcat ttagttcgat cttatgataa tccactaatt tcaatgcctg
     6241 atcgatttct tcttgggaag gttttctggc gtcatcaaaa ccgattcgcc ataattgtgt
     6301 gattgcacca atcgccatat cgaatccgcc ttgagaatct tggctatatt cgtaggcccg
     6361 cttgactaac gtgtagacgt catcagaaac ttttactggt ttgatcccag cttgctcatt
     6421 cacttcatcg atctcagaac ctttttgatt gatcgtgatt ttatcaccaa gttcttttac
     6481 tcgatcgaag gcaggtttca atgctgattc tttgccttca tcatacacac ggatacgaac
     6541 gtaagtccct aacaagaact gttcatcaga atatggatct ttcaatatct tggtagctgc
     6601 tgaactttca gtggttgttt tatttgctga gttgctgcag ccagttagca ccataaaaga
     6661 gatgcccagt gtgattaagg aaaatagtag ttttttccgc atctttttcc tctcttcatt
     6721 ttgaaaggtg agacgactgc ctaatctggc tatcgtccca ccctccaact tttattagta
     6781 tgattatgtg attatttttc tgttacgata ttatctactt cgattgtttg agtgtcgcct
     6841 ttttcagctg cattcactaa ttgttgtgcg tacatgatga atgaatgtga gctgtgtgta
     6901 gcacctgtaa caacttctac attagcaggt gagccatctt cttcgcccat tgctttgacg
     6961 aattcgtcgt tcagagtagg gatgtaagtt tttggttctg taccgctttt tgctttcata
     7021 ttttcattat attctgtatc atcttgtttt gatttaccgt ctttgtcgat ataatcaaag
     7081 tttgattcag ttactttgcc gccagcaact gtcatttcga attgcacacg gtagccgtta
     7141 gagtagttct tttcttcaag cttgtaagtg ccgtctttca aatcagcacc attgtcgatt
     7201 tcgattgtat ctgtgttacc agcttgagca gcttggatca attgttgtgc gtagttttgg
     7261 aagctttcag atgagtgagt agctccagtt acgacttcca caccgcttgc gctttgtgct
     7321 tttaagaatt ggtcattcaa ctcaggaatg tatttttctg ggtttgtgcc agattttgct
     7381 tccatgtttt tgttgtattc tgcatctttt gtttttgatt cgccgttttc attgacgtta
     7441 tcatattttg attcagtgat tttgccgtct tttacaacca tttcaaagac tgcacggtaa
     7501 ccatttgaat aatttttttc ttctaattta tatgtgccgt cttgcaaatc tccgccagct
     7561 acaacttttg cagatgattc tgttgtgctt tttgcagtcg aagattgtgc tgtagatgat
     7621 tcagaagaag agtttgagct tccgttgtca tctgcgccgc aagcgcctaa cactagagct
     7681 gaaaatgcta atacggcaaa tccagttaca aaacgttttt tcttcatttt aataccccac
     7741 cattccattt ctagtccaca atttttcttt gtgattcatt atacattcta atctttttag
     7801 cctataagtc aactattttt gataaataac ccatgtaata tttcaaacgt tttctttcag
     7861 caaatttcgt gaaaaaacgc tttattttaa aatttgaaaa aaatccaaaa ggagttagac
     7921 aaaagaacag aacatctata taatatatgg agattgtgta ataagtatca aactaaaaga
     7981 attatcatat attcattata tagatagtct ataaggagcg gatgatcaat ggcaaagcaa
     8041 aatattgtcg ttgtaggagc aggatatgcc ggtgtttcag ctacaaaata cttggcaaag
     8101 aaactgaaaa aagaagatgt agcaattact ctgatcgatc gtcattcgta ccatactatg
     8161 atgaccgagt tgcatgaagt agctggcgga cgtgtagaac cagaagcaat ccaatacgat
     8221 ctgcagcatt tgtttgctcg tcaaaagaat gtacaacttg tcacagacac tgtaatcggt
     8281 atcgataaag aacgaaaaat cgtcaagacc aaattaggtt cctatccttt tgatcagctg
     8341 atccttggaa tgggcggaga gccgaatgac tttggtacac ctggtgtcaa agcacacgga
     8401 ttcacccttt ggtcatttga agattctgta aggattcggg aacatattga gaagacagta
     8461 gcaaaagctg cattggaacc agatgatgaa ctgcgtaaag ccatgctgac tttcgttgtc
     8521 tgtggttctg gatttaccgg tattgaaatg attggggaac tgatcgactg gaaaaatcgc
     8581 ctagccaaag attataaatt agatccaaac gaaatcacac tgatggtcgt agaggcaatg
     8641 ccgacgatct tgaatatgtt ggatcgaaat gatgctgcaa aagctgaacg ctatttgcgt
     8701 aagcacaacg tggaattatt attgaatgca ccaatcgtag aagtggctgc tgatcatatc
     8761 aaattgaaag acggacgcaa cattccgact cacacactga tttggacagc tggtgtcaaa
     8821 ggaacttctg acgctgctga ctttggatta gaatcagcac gcggacagcg gttgatcgcc
     8881 aatgaatata tgcaggcaaa aggctacgaa gataaaaaca tctatatcat tggtgattta
     8941 gtcttttatg aagaattccc aggaacaccg acaccgcaga tcgttcaagc tgctgaacaa
     9001 acaggacaca cagccgcagc gaatatcgtg gcttcgatca aaggcggaga aaagcataaa
     9061 tttaaaggaa actatcaagg atttatggtt tctattggtg ccaaatgggg ggtagctaac
     9121 ctatttgata agatccattt aagcggattt ttagctatcg taatgaaaca tatcgtgaat
     9181 ttgaaatact tctttgatat tcgttctggt tattatatgt tccaatattt aatgcatgag
     9241 tttttccata tcaaagatga tcgcaacgtg actcgaggac attcttctcg ttacggcaat
     9301 gtcttatgga gtgtgccact tcgtattttc tatggtttag tttggttcgt tgaagcttcc
     9361 aaaaaaatca ttgggaatgg tgaagtgttc aaaccaagta cttggtttgg tgaaggatca
     9421 tggttcactg gaaacagtgt gttcccttgg acatgggaat acaatgtaac agatgttgcc
     9481 agtggtgctt ctgctgctgg tgatgcaacc acagctgcca gcggagcagg ggcaagtgcc
     9541 ggagaagcag cgactcaagc tgcacatttt ggtttgagtt atgcatacgg tgaagaacca
     9601 atggctgtga ttgagaagtc accagactgg ttcacaaaga tcatgcaagt gatcatgcca
     9661 aacaatgatg tagcaatgtt cttccaaaaa ttcatggtat tctttgaaat tgccttggca
     9721 ttagcattga ttgctggatt attcacatgg ctatgcagtg cgacaacgat tgtattggtg
     9781 atcacgttct gcttatcagg catgttttac tgggtaaaca tttggttcat ttttgttgct
     9841 ttcgcattga tgaacggttc cggacgtgcg attgggttgg atcgctgggt aattccatgg
     9901 ctacaacgta cactaggtaa ttggtggtac ggtagaccaa gttcccagta cggcaagaaa
     9961 taaatagata aagaacgtta tttgaatgca agatttcaat agaaaggggc tgagacaaac
    10021 cgtcacagcc tcctttttat gtaaaattaa aggaataaga tgcccgagat aagcctccat
    10081 gagcaagaca caggtgtcac aacctctgtt acaacctctt gttataaacg gtgttccaga
    10141 agtagcttct tcgaaaaata agccgaaatt tcacaaaaac ttgaaaagta attttcgtaa
    10201 attccttctt atttcttgaa gttgcccact tctgtcacaa cctcttgtta taaacggtgt
    10261 tccacctgct gcttctcggt aataagccca aagaattaga aaacatggaa agctgttttc
    10321 tattcttcgt tcttattact caaagcaaga cacaggtgtc acaacctctt gtttttatgc
    10381 taaaattggg tgagttaatc tggaaaaagg agaacaaaaa tgagccttaa agaatttgct
    10441 gaaaaaagtc tgctcaaacc ttgggatgtt tttattattt tgcttttgac tgttctttct
    10501 tttttaccag tcatgatttt tggtcttcag caaaacaccg caacagcaga aaaagaagcg
    10561 gtactgcgtg tggatggcac agaaatcaaa acattccctt tgatggctgg agaagagagc
    10621 aacacatata cttacaaaga tgaacacgga gattataact tgatcgagat agatggcgat
    10681 cgagtacgga taaaagaagc agattgcgat gaccagatct gcgtacgtcg aggctgggca
    10741 acgaaaaatg gcgaaaccat cgtctgtttg ccacataaat tagtgatcga agtccgttca
    10801 acagatggag gtgacgaaga cagtctgatt tattagacct tcacacacca tgagtaaatt
    10861 acaaaaaatg atttatattt cgctacttgt tgcacaagga gtgatcattg gtttattgga
    10921 aaatatgatc ccttatcctt tttcttttgc gcctggtgca aaactaggct tagccaatct
    10981 aatcacgatc gttgccattt ttaccatgaa aaaacaagac agcttcttac tactttggct
    11041 acgactattc ctgactactt tgctaggcgg aacgatttcc acattccttt atagtatgag
    11101 tggtgctttg cttagttatt ttggaatgtt attggtcaag caattaggac caaaacgtgt
    11161 cagtatcatc ggaattagtg cggccggcgg tttcatgcat aatgtcggac agcttttgat
    11221 ggcttctttc attgcacaat catggacagt catgttgtac ttgccagtcc tttcagtttt
    11281 agggatttta tcagggattg caatcgggat cgcagcgaat tatcttttac gacatgtgca
    11341 gacacttcaa cgattccaaa aagattatga gcagggaacc aaacaaaaat ggagcccaat
    11401 gtttaccaaa aaataatcgt cacacaagga gtttttttat gaagctacat ccaatgtggg
    11461 aaaactatcc tgctttaagt aaagaattga cagaaacact aaaacggatg gaagaaactg
    11521 tccgcctgaa aaacaaacct gtcgaacaag caatcaaaga aacgatccat gccggcggaa
    11581 agctgttgcg tcctgcctat cagctattgt tctcacaatt tggaccagag caagatcgag
    11641 acaaagccat cgctttggca gcttctatcg aaatgctcca tacggccaca ctgatccatg
    11701 atgatatcgt tgatgaagcg gatctgcgac gtggtcagcc taatatccgt agtcagtttg
    11761 gtaatgtcgt ttctgtctat gcaggagact atttatttgt ctgctgtttt aaactattgt
    11821 ctgattactc cacttccttg aaaagtctcc aattgaattc tcgcagtatg gaaaaagtat
    11881 tgaatggcga attagggcag atggatgatc gctataatta tgaactttct gtaaaagaat
    11941 atttggaaaa tatttcaggt aagacagctg aactgttcca attaagttgt tctgttggcg
    12001 cttttgaaag tggaacaagc gagcgcttcg caaaaaaagc aggagatatc ggactttcta
    12061 tcgggatggc ctttcagatt atcgatgata ttttagatta tacaaaagag agccaggaaa
    12121 tcggcaaacc tgtactagaa gacatgcgtc aaggggtcta ttctttaccg ttgatctact
    12181 ctttgcaaaa aaacaaacca aagctactac cttatttaga gaagaaagcg gcaatgacag
    12241 aagaagatgt cgacgccgta cgcaaaatcg ttgaacatac aggaggcgtg gaagaagcta
    12301 ggaagttagc ggcctcttac acagaaaagg ctttgaagga aataaagaaa ttacctgcca
    12361 cctctcttcg aacaaaagag aatctctttt cccttaccca actgattttg gatcgcaaag
    12421 actgaatagg gaaaggttcg atacgtaaat aaatttctca gaaggaaatc tacgtgtttt
    12481 ttgacaaatt gaattgtagt tcttgtattt atgataaaaa gcaaaaataa agagcaacgt
    12541 ccgttagcga cgttgctctt ttttatcttg aatatttttt taccttttcg atttcttata
    12601 actcataaaa gtgattctta aaccgattac aagtaatata acgcctgcga gtgttatcca
    12661 attattcgat tgacttccag ttttaggaag tgctcttttt gatttgtttc cattgtcatt
    12721 gcttgtttta ttagttaagt taaagttcgt cctgttatcc gaacttgttt ttgagaagga
    12781 gacacttgca tgacgtgtat cgcttgtggt agacgacgtg tctgtactat cgcttgaagt
    12841 tgatgaatct gagctatcac ttgtggtaga ggatgtatct gtactgtcac ttgaagctga
    12901 cgtgtccgtg ctatcacttg tagtggtaga tgaatctgag ctatcacttg tggtagaggt
    12961 agtatctgta ctgtcacttg aagctgacgt gtccgtgcta tcacttgttg tggtagatgt
    13021 atccgtactg tcgcttgaag ctgacgtgtc cgtgct
//
LOCUS       DACKAC010000076        12747 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.077, whole genome shotgun sequence.
ACCESSION   DACKAC010000076 DACKAC010000000
VERSION     DACKAC010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.077"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(84..263)
                     /gene="rpmF"
                     /locus_tag="I0B46_002313"
     CDS             complement(84..263)
                     /gene="rpmF"
                     /locus_tag="I0B46_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAR2084571.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(321..890)
                     /locus_tag="I0B46_002314"
     CDS             complement(321..890)
                     /locus_tag="I0B46_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAR2084572.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1076..1978
                     /locus_tag="I0B46_002315"
     CDS             1076..1978
                     /locus_tag="I0B46_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAR2084573.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2013..3797)
                     /gene="pyk"
                     /locus_tag="I0B46_002316"
     CDS             complement(2013..3797)
                     /gene="pyk"
                     /locus_tag="I0B46_002316"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAR2084574.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3871..4833)
                     /gene="pfkA"
                     /locus_tag="I0B46_002317"
     CDS             complement(3871..4833)
                     /gene="pfkA"
                     /locus_tag="I0B46_002317"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAR2084575.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5001..8315)
                     /gene="dnaE"
                     /locus_tag="I0B46_002318"
     CDS             complement(5001..8315)
                     /gene="dnaE"
                     /locus_tag="I0B46_002318"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAR2084576.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8546..8731
                     /locus_tag="I0B46_002319"
     CDS             8546..8731
                     /locus_tag="I0B46_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAR2084577.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8793..9989)
                     /locus_tag="I0B46_002320"
     CDS             complement(8793..9989)
                     /locus_tag="I0B46_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAR2084578.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10159..11595)
                     /locus_tag="I0B46_002321"
     CDS             complement(10159..11595)
                     /locus_tag="I0B46_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAR2084579.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVVTTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11888..12697
                     /locus_tag="I0B46_002322"
     CDS             11888..12697
                     /locus_tag="I0B46_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAR2084580.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3806 a         2676 c         2250 g         4015 t
ORIGIN      
        1 ttttttattt accgactaca aaaaaataaa cctcaaggct ccatggttag ccttgaggtt
       61 ttacattttc taattaaaat gtattaagct tcttttgaca ttacgtcctt accatcatag
      121 taaccacaat ttgcacatac gtgatggctt tttttcattt caccacagtt tggacattcg
      181 tttaaccctt tgattgtcaa tttgtagtgt gtacgacgtt ttgctttttt agcttttgat
      241 gttcttctag ctggtactgc cattttgcta cacctccttg taaagtcttg tatctaaatt
      301 agacacaata tattccaatc ttacttgtta tcgtcgtctt ttgatttaga atcaaaaagt
      361 tcagatagtt ttgcaagacg aggatcaatt ttattctcgg cttcttccat ttttctgcgc
      421 tggtattctt cttcggaaac aacttcccaa tcattgcctt ttggaagttc tgttgctgct
      481 tcctcttctt ttgtcaatac ccgcattgga atcgccaata agatgttgtc ttcaatcgac
      541 tctgctaaat tcaataatgg accttcaatt agcaaaatct catctgcaga catttgttca
      601 tctctacttt ggaattgttc ttctgtcatg aatacttcgt ctacagaaaa atccatgggc
      661 agttcaacag gttctagtga acgtgttgaa ggaactgtca aagtcgtctg cacagtgtaa
      721 tgaacgatat agcctttctt atcaactgag accaaacctt cgacttccac tggcgcaaca
      781 tcaagtatta aattgtctcg cttcatgaga gcggctttca agtcgagcgt ctcatgaaaa
      841 gttaacggtg tctcctggta ttttctcaat tccaataaag accatttcat ttttcatcac
      901 tcctaagcaa caaaagcaat tatacagggc aaaaagcatg ttgtcaatga aattttcttg
      961 acaccttgca gttcataaaa aaacagaaaa atctttatta gaaagcctct gtttgctatc
     1021 atcctcagaa tcatttacaa taatcattat tccattaaag gagcggatga tcttcatgaa
     1081 cacgaaagat atcgaatact atgtccaaat cgtcaaacaa aaaaatttta cgaaagtggc
     1141 taattatttc cgtgtgagcc aacctactat tacatatgca ttacaacgtc ttgaaaaaga
     1201 gtttgacacc gtacttatta caagagatcg atcgcatcac gaattaatcg tcacgccttc
     1261 tggaaagcag ctgtttctcc atgcacaaac cattctccaa gagatttcta ctgcaagagc
     1321 tgaaatcact cagttgcaaa agaaaaagct tcgttttggc atgcctccta ttatcggcaa
     1381 tgaatacttt cctaaactgt caagttactt tatcaagcat gaattgatga atcaaattga
     1441 gattaccgat ggaggctctc gggacttata cgggatgatc cgacaaggga ggatcgattt
     1501 agctgtatta ggctcaacac agccaatcgt ggatcaatat ttagaaacag agttactttt
     1561 ggacaaacgt ttcatgatcg tcgtgccacc tacacatcct ctttccaagc aggatactgt
     1621 ctctttcaaa gatatagcag atgaacagtt tgtactattg aacgaacatt atgtccaccc
     1681 cgcagcattc actaagcttt ctaaacaaac acatactcat ccaaaagttg tttatcaaag
     1741 caatgacttg acgattttaa agagtatggt ccgagaaaat gtgggtatcg gttttttggc
     1801 agagattgct gtccatcctg aagatcattt agtttgcctc cctctttcag atgaagttca
     1861 gcctcgcttc ttgatttcat tggcaaaacg aacacagcag atcgtcacgc ctcttcaaga
     1921 acaagtagtc caagttatcc atgaattcgt tacagcacaa aaaaacagct cttcgtaaaa
     1981 gaagagctgt ttttattttt tattaagcta aattagatag ctgttgtttc gccacggtaa
     2041 acgatgccgc gacgtgaatc aacggtaatc aattcacctt ctgtaattgt tgttgttgcg
     2101 cctttagcac caacgattac aggaatatct tgtgcaattc ctacaactgc agcgtgagaa
     2161 gtcaatccgc cttcttcaac gataacagca gctgcttttt cgattgcagg catatattct
     2221 ttgtcagttg atggtacaac tagaaccatt ccgtcttttg ctttttttac agcttcttca
     2281 gcagatgaag caataactgc atttgcaacg acagattttt gtccaacgcc ttgagcttct
     2341 aataatttag aaccaatcat ttgaattttc atcacgttag ttgttccgcg ttcaccaact
     2401 ggtacaccag cagtgatcaa gattaaatcg ccttctgatg cgaaacctaa ttcaacagct
     2461 tttttagctg ctagttcaaa catttcatca gttgttgatg gtttttcagc aactgttggg
     2521 taaacgcccc agtttaacat caaaccacgt tttgtgcgtt cgtcaaaagt aacagccaag
     2581 atatctgcat ctggacgata ttttgaaatc atttttgcag tgtatcctga ttcagttgca
     2641 gcaacgattg ttttcacgcc tagatttttt gcagcacgag ctacagataa accgattgtt
     2701 tcagttacat ctgttttatc gaattcgttc aattggaatg tacctaattc tgataatgca
     2761 ttttcagctt cttggtcgat acgtgccatt gttgaaacag cttcaactgg gtagtccccg
     2821 tttgcagatt caccagatag cattgttgca tctgtgccat caaatacagc gttagcaacg
     2881 tcagaagctt ccgcacgtgt tggacgtggg ttttcttgca ttgattctaa catttgtgta
     2941 gcagtgataa ctgggatacc agcagcatta cattttttga taatgcgttt ttgaaccatt
     3001 ggtactagtt cagctgggat ttcaacaccc atatctccgc gggcaatcat aacaccgtca
     3061 gaaactttga tgatttcgtc aatgttgtcg ataccttctt gagattcgat tttagggaag
     3121 atttgaacat gagtcatgtc tttttcttct aagatttcgc ggatatctag aacgtcttgt
     3181 gctttacgta caaaacttgc agcgataaag tcgatatcgt tgtctaaacc aaaacgaata
     3241 tcgtcagcat cttttggagt aatacctggc aaactaatag aaacgcctgg tgcatttacc
     3301 cctttacgtg atcctagcat acctgcattt ttaacttcta cgactaattc acggtttgct
     3361 tcgtcttttt caatgatttg catatcgatc aaaccatcat caaataaaat gtgtccgcca
     3421 acgtgaacat cgtcaaataa tcctgggtaa gtaaccgcga ttttttcttt tgaacctaca
     3481 tgttcaggat ccatagagat gcgtgtttga tctcctactt caaatttgat gtatcctgca
     3541 cgaccaaaat cagcttctgt tgtgccttga acagttgtac ggatttcagc tccttttgta
     3601 tctaaaagaa taccgacatc ttttcctgtc tttttaactg cttcacggac catagtcata
     3661 cgtgataatt gttcttcatg gtcaccatga gaaaaattaa agcggaagac atttgcccca
     3721 gattcaatca attgagaaat aatttctact gagttacttg ctggtcctaa tgtactaacg
     3781 attttggttt ttttcattta aaaaactctc ccattcaatt ttgtttattt aaagctgtac
     3841 acagcttaaa tagcttggtt tctttcactc ttaaaatgaa agtgaattat ttagatcata
     3901 tagtgataag tctggtttat gtttgttgtt ttcaagtgta tcaatgatgt cagacgcaac
     3961 gatttcgttg tcatggatac cgacacataa tccacctttt ccttcttgaa gcaattcgac
     4021 agcgtaagca ccgaatttac ttgccaacac gcggtcgcgt gcgcttggtg ctccaccacg
     4081 gacaacgtgt cccaagatag atacacgtgt atgatagtca ccaaattctg aaagtttctc
     4141 agcgaattca tttccgccca ttacaccttc agccaagatg atcaagcaat gtttcttccc
     4201 gcgatcgcgg ccttcttgga tttttttagc aactgaagcc atatcaaaat catgttctgg
     4261 gatgatgatt tcatccgctc cgccagccac acctgaccat aaagcgatgt ctcctgcatt
     4321 gcgtcccatt acttcgataa cgaatgtacg aacatgagaa gtcgctgtgt cacgaatacg
     4381 gtcaattgat tctagtactg tattgattgc tgtatcaaaa ccgattgtga aatcagtccc
     4441 aggaatatca ttatcgattg ttccaggaat acctactgct gggaaaccgc gttttgtcaa
     4501 agccattgcg ccatgataag agccgtctcc tccgataacc actagacctt cgattccgaa
     4561 ttttttcaat tgttcgattc cttttaattg accttcttca gttgcaaatt ctggataacg
     4621 tgcagaatac aagaatgtac caccacgttg gatcttgtca cctacatctg ccacatcaag
     4681 acgacgaata tctccagcaa ctaaaccggc aaaaccataa ttgatcccgt atacttccag
     4741 tccttcgtaa atacctttac gaacaacggc acgaacagct gcattcattc caggtgcgtc
     4801 tccgccactg gtcaaaattc cgatgcgttt cataataaac attcacctca ttaaaatact
     4861 ttatcctaag tatgacttag gtttacaacc atttttgcgt taaccacgac taacattcta
     4921 acattatttt tctcacttgt cttgatttac ggataaaaaa atgaaaaaaa atgaaagcaa
     4981 gtagtatttt ttaccgtaag ttatttgaaa accacattat cttcgccaag cagataaata
     5041 agttgcccct ttatactctt gctgtcatcc acccaataag catctgacag aactactttt
     5101 cgttgcgttt tttcgtgata cataactact ggtatatatc ctgggtgttt ctgaaaaatc
     5161 acttgtaatt gtttcattat ctcgggatct tcccgttctt ctgtaatacg caaatagcaa
     5221 gtttgtgaag gatactgggc ttctaactgg ttaggatcag caaagctttc tccaattagt
     5281 tgcagttcct gattatattt gctgatttcg ctccgccctt gaacaaagat cactcgatca
     5341 acggcaaaca aattacgata tttacgataa agagttggaa aaactgtgac agaaatttct
     5401 ccactactgt cggttccttc aacaaaggcc atcagttctc ctttttttgt ccgaatttct
     5461 ctaacttcct tgatcatcac taataaattg actggttgat tagggacgat atctgtaatc
     5521 agctgaactt gtttagctaa acgaagacga tcaaaccctt ctgcaggatg acctgacaaa
     5581 taaataccta agtactcctc ttccagcttc aagcgttctt ctagtgtata gtcagcgact
     5641 tcttcttctt ttaatgccat gattcccagc aagtcaagac ttccaccact gtaaacgata
     5701 ttttggattt ttccttcgag atcaagcatc aattgtttcc tgttaggaga taattcatca
     5761 aaaactccta ctgctgtcag tgattgcagt aactctaatt tcagccaacg cttatcgatc
     5821 cgtatgagga attgatccac tgactggaac gggccatttt gttttctttc gtttaatata
     5881 tcttctacaa aatcacgccg aatacctttt atcgctgtca gtccgaatct gatttcttcc
     5941 ttattgacca actcaaaact ggaattactt tgattaatgg acggcgagga aagtttgagt
     6001 ttatttttac gtgcttctgc aatatattct ttcaattttg atgtgttatg ccgaacagaa
     6061 ttcatcaggc tggtaaagaa agcacctgga tgatgggctt ttagatacgc catttggaat
     6121 cccacgaatg aataagcaaa tgcatgtgac cgattgaatc catagtttgc aaatttttcg
     6181 atatattcat atacggtcgc tgctgttttt tccgaatatc cctgttgtaa tgctcctgat
     6241 acaaaatgcg acttttcttc atctaatacg tcttttttct ttttactcac tgcccgtcgc
     6301 agaatatctg actgccctaa tgtaaatcca gccatagtcg aggcgatctg catgatttgt
     6361 tcctgataga cgatcactcc atatgtgttt ttcaaaatcg ggatcagtga atcatctggg
     6421 tagcgaatgg cttcttttcc tttcttcctg gcaatgaagg tatctatatt ttgcataggg
     6481 cctggtcgat acaatgcatt gactgcggca atatcttcga tgttttctgg atctaatctt
     6541 cgtaaaacgt tccggatacc agcagattca aattgaaata ctcctgaggt ttcgcctcgt
     6601 tgaaacaaga gcagcgtttt tgcatcattt agcgggatct cttttaatcg gatctcatac
     6661 ccttctactc gcttaacagc tttcaaagta aaatcaataa tcgataagtt gcgtagtcct
     6721 aaaaagtcca ttttcaaaag accgattgct tctacctcat tcatcgtata ctgggtcaga
     6781 aaagcttcat cagaaccgcg ttggagcgga actagttcaa gtagattctt atcgctgata
     6841 actacacctg ctgcgtgagt cgaaacatgt cttggcaatc cttctaaaat cactgcagtt
     6901 ttgaataaaa gttggttttt ttcgcttgcc tgaactaatt ctactaattg atgcgaagtt
     6961 ttataagctt gggtcaatgt gattttcaat tgattgggta ttgcttttga ccagcggttt
     7021 gcttcacttt gcgataaacc aaacactcta gctgcatctc taagaaccat ttttgctgcc
     7081 attgtgccaa atgttgcgat ttgtgccata tgatattgtc catacttttc tcgaacataa
     7141 ttcaatactt cttcacgtcg attgtcagga atatccaaat cgatatctgg catcgtaaag
     7201 cgttcggggt ttaaaaagcg ttcgaacagc aaatcatatt ctaatggatc cacatctgtg
     7261 atttctaaca cataggctac taatgaaccc gcagcagaac cacgacctgc accggtaacg
     7321 attttgtttc tatgagcaaa agccattaca tcccatacga tcaagaaata atcatcgaat
     7381 cccatttgat ggatgacaga taactcgtag tccaaacgtt cttggtattc ctttgtatag
     7441 tcagtgattc ggcgttccac ttgcgaatag cataactctc ttaaatattc tccagcttgt
     7501 tttccattag gaactggata atgcggaagt aatttctgat ggaactggaa ttcaaatgaa
     7561 atcgattcag ctaaacgctg ggcgcctatt acagcttggg aaagacccgc tttttcgaat
     7621 cgtttgatca tttcttgttc tgaaagaaga tagacatttg ctgttagtgg taaagatgca
     7681 gataaatttt ctaaaggtat ttggcttccc tctttgatat gtttcatcac ttcaagtgca
     7741 aaaagttctt cttgttgtaa actagcgatg ggatgtaatg cacaaagcga ataattttct
     7801 tttatcacat actgatacca ttcaggcaat tgatcaggcg tgttttctgt caagccgata
     7861 taaaaatttc tagtaaaaac attttcaagt aaaaactgcc aatgaccgaa aaagttatcc
     7921 tctttattga aagcctcaga aacagaatga tttgccggaa caacaaacgc tagatggtcg
     7981 gtttctgtca tttctgcaag ttccaatttt tcttggatca tttttcggct ggaaagtttc
     8041 atcagctgct gataacctat ttcatctttt gcgtacgcaa gcaagccata atttttttct
     8101 tcataagtat aactaagatg taatccaatg attggctgta ctccttcttc tacacaaata
     8161 cgatagaatt caggtgcacc gtataagaca tctttatccg ttatggctaa tgaagtgtag
     8221 ccttgttttt ttgcttcttg tacatattct cgaatacgta tcgtactttg taaaagagaa
     8281 taagaagtaa ttgtataaag ttgtgggaaa aacatcttgc attccgcctt ttttttcgtt
     8341 aaaattcact agcgttaaaa ggtttgatcc ttttactttc attaagatag caggcgctga
     8401 agttgattat tccacttcat ttgcttgctt gtaatctgta ggaatgagtt ctttcgcaag
     8461 aactcaaatt ttattgagat ttggtctcgt ttctttacat tcaaagaaaa tctggtaaaa
     8521 ttaagataga aaagaggtgt tctttatgcg atatatcgtt acattattct gggctgtcgt
     8581 tctaggtcaa gtagtcggtt atattggtgc tgcattaaca agcggaacat atgattttac
     8641 tttgacaaca atcatttcat tcattgcggg tgtcattatc cttttgattg gtgcagttgc
     8701 gccaagaaaa gaaacctctg cccattcgta aaaaaacagc caagagcttg agatttctca
     8761 agctcttttt tattttctac tcctcaccac cattataatc tgctgcgaag aactttacga
     8821 aagttgaata aagaaagaag aatatttgac agaacaaaca aacttgtcac aataaaaaca
     8881 gcactgtaat tataagcatg cgcgactgtc gatcctagca tgggacctaa aacttgtcca
     8941 aagttgctaa acatctgatt gtagctatag attcggctga ctccttcttg aggcgtaatc
     9001 ttactgatca gtgtattgac agaaggcatc aaagcgccag ttgaaaagcc taaaataaag
     9061 cgaagtatac ctaattggag cggtgtagtg acgaatgcca taggtaaata acagcaaaat
     9121 gataaaatga gtcctccaat tagaactttt tgattcccaa tcttatcccc tagttttcct
     9181 agtttcggtg aagaaaagac agcggaaaca ccagctatcg aaacaatcaa gccgctgaca
     9241 aaaaggatat tttctgtact tccgctcaat tcacgaatat ataaggtcaa aatagggctg
     9301 atcgtagtga ttcctatctg taaaatcaag gtcgtaatga agagaccgat caaaatagag
     9361 agatgatcca tttttgaaaa aatttctttg gtgcttatca gatctttttt ctcgattgga
     9421 tggaaatctt ctttgactaa gaaaatggtc aataccgttg tgatcatcaa caaagcccct
     9481 gtgatcaaga atacattttc cataccaaac cactgagcca gtgcaccgcc cattgaaggt
     9541 ccgatcagat ttccagcaat cgctccggta gacaatgttc ctaacgccca tccgctcttt
     9601 tctctaggtg cctgtgaagc aatcatcgct gtggcatttg gaatatatcc agacaaaatc
     9661 ccattccaaa accgcatgat caaaagccaa tacgcgtttg gtacaaaagc caaagatccc
     9721 attgttaatg tcatccctgc tgctgcacgg atcatcatga gttttcggcc ttttcgatca
     9781 gctagatttc cccagattgg tgcaaccacg gcagatgcaa aagcagtcac agagattgct
     9841 agtcccgaaa acaattcgat ttgatctttt ggtgttccta gttgctcaac ataaactggt
     9901 atgaatggca taactaaact aatacttgca ccagtgaaaa aacagccaat ccacgcaatc
     9961 actaaatttt ttctccaatt aatctccatc tatcatccct ctttcgtctc ttttatcata
    10021 cctattttcg aataaacaag caacgatctc gccagtaaaa agagaaaaat cccgcaaagg
    10081 tcattttata tgtaacgttc tttttatgta aaacaaaaag agatcagaac aaatactgtt
    10141 ccaatccctt ctctttagtt aaattgttgc tagaatgatg aagttgataa tgaataaaat
    10201 atcaacgatc cacaaagcaa cagaaacttg attgtatttt cctttgatta ctttcacaat
    10261 tgtatagaaa ataaatcctg ctgcaatccc ataggaaatg ctatagcaca atcccataaa
    10321 gatcgatgca aagaaggccg gtaaagcttc ttccatatct agccatttga tgtctgcaaa
    10381 agaagccatc atcattactc caactaaaat caatgctggc gcagttgctt gatttggtac
    10441 aatggcgatc agtggagaga ataaactact taatgcgaat agcactgcca ctactactga
    10501 agtcaaacca gtacgtccac cggccccgat cccagctgca gattcgacat aagttgttgt
    10561 attcgatgta ccaaagatag cccccacaga agtagcgatc gcatcggcaa atagtgcttt
    10621 atccattttt gttttgaacc cacgaccatc ttccaacgcg atctcgtctt cttttgagaa
    10681 aatacctgtg cggcgtcctg taccgataaa tgttccgatc gtatcgaaag tatcagataa
    10741 actgaatgcg atgatcgtca tcaatacttg aggaatcttg gaagaatcac tgaataaaga
    10801 ttgcatgcct tcagaaccaa atgctgcacc gaaagttgtt cctaattctt taaatgaatt
    10861 acctaacgaa ttcgtttgcc aatcaatgga acttaaatca acaacaccca taaaaatacc
    10921 aaggactgtg gtcactacga taccgataag aatagcccca cggacatttt ttacaactaa
    10981 aatggctgtc agtaccaaac caatcacagc caataagata ggagcgttgt caaagttggc
    11041 taacgcaggg acgattccac catttatcgt aacatttgtc gcttttccgc cttcaacgac
    11101 agagctcgaa atagctgact ggtctgctga aaaagataag aatcctgcat ttttgattcc
    11161 gacatacgca acgaaaatac cgatcccgcc accaatcgca tgttgaagac tttcgggaat
    11221 cgcccgaata atcatttttc gaatatttgt gacagtaata aaaatattga ttaatccgca
    11281 aataaacacc attgccaagg cttgttgcca agaataacca agcccaaaaa cgaccgtaaa
    11341 agtgaaaaat gcgttcaatc ccataccagg tgcctgagca tatggtacat ttgcgaacaa
    11401 tcccattacc agcgtaccaa taatcgatgc aatgattgtc gctagaaata ctgcttggaa
    11461 gggcatacct gatgctgaca aaatcgtcgg atttacgaat aagatataac tcatggcaaa
    11521 gaatgtcgtt actcccgcca ttatttcagt tgatacattt gtattgtttt tctttaaacc
    11581 gaaaaacttt tccataaaaa gttccactcc ctgaattttt ttctgatgga aaactataat
    11641 aataaaaaat aagtaaaata gggcaaatcc atcgaacaat ttcaaattat atagacgttt
    11701 cattcgcttt tcaacgtttt tttaacaaaa aaagcgaaaa taatacttaa cgttcgtgtt
    11761 tagcttatgt ttagctattt atatagccat aactgtagtt tttgttagga atagctgatt
    11821 gctcttgtta attacctaaa atatgctaga ctaaaacaaa tgctagttga ggaaggaagg
    11881 acccttattg aataaaaaat taattgccat cgatctggac ggaactaccc tcaatcaaga
    11941 ttcgatgatt acatctaaaa caaaagagac tctaaaaaaa gcaatcaatg ccggtcatca
    12001 tgtcagtatt gctacaggac gcccattccg aatgagccat cagttttacc aacaattaga
    12061 attaacaaca ccgatggtta attttaacgg cgcattagta cacatcccta atcagcactg
    12121 ggatggtgaa agagaaacgt tgatcaatcg tgaaatcgct tttgaaatct tatcgcaaaa
    12181 aaagcagcta aatttagatt ttatcgctgc tgaaaatcgt gatacttttt ttattgacag
    12241 ttttgatttc tttgatgaaa aaatttttgc ttcttctcgt ccaggagaga agaatttgct
    12301 ttctcctaaa aatttgacga caaatcctac atctttgctt gtccgaacag acaaacgttt
    12361 tgcaggagct gtatctgctg aattaacacg tcaatttggc agttatgtcg atgttcggac
    12421 atggggtgga cctactgcca tcttagagat tgtttcaaaa ggaatccaaa aagcaaaagg
    12481 cgtgcaagag attgctaact acctgtctat cgatcaaaaa gacgtcattg cctttggtga
    12541 cgagcataat gatttggaat tgttggatta tgcaggttgg ggagtagcta tggctaacgg
    12601 tactgaccaa ctcaaaggca ttgcaaatga tgtcactcct ttgagcaatc aggaagacgg
    12661 tttagctgtt tatctggaaa aactacttaa actataatag agaattagat tccacaaaaa
    12721 agaggttgtg acaatttttg tcacaac
//
LOCUS       DACKAC010000077        12674 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.078, whole genome shotgun sequence.
ACCESSION   DACKAC010000077 DACKAC010000000
VERSION     DACKAC010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12674
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.078"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(147..1178)
                     /gene="queA"
                     /locus_tag="I0B46_002323"
     CDS             complement(147..1178)
                     /gene="queA"
                     /locus_tag="I0B46_002323"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAR2084581.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKIGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            1371..2546
                     /locus_tag="I0B46_002324"
     CDS             1371..2546
                     /locus_tag="I0B46_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAR2084582.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESDTPEESKEV"
     gene            2550..3191
                     /locus_tag="I0B46_002325"
     CDS             2550..3191
                     /locus_tag="I0B46_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAR2084583.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            3188..4105
                     /locus_tag="I0B46_002326"
     CDS             3188..4105
                     /locus_tag="I0B46_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAR2084584.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYDQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVNNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            4102..4773
                     /locus_tag="I0B46_002327"
     CDS             4102..4773
                     /locus_tag="I0B46_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAR2084585.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATDGTSIILAGALPTALMVIVTDWLLGILERRLDPA
                     SRTSR"
     gene            complement(4969..5803)
                     /locus_tag="I0B46_002328"
                     /pseudo
     CDS             complement(4969..5803)
                     /locus_tag="I0B46_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016619675.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6004..6270)
                     /locus_tag="I0B46_002329"
     CDS             complement(6004..6270)
                     /locus_tag="I0B46_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAR2084586.1"
                     /translation="MSNYTVAIKNSATVDLRKIKQSNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(6270..6533)
                     /locus_tag="I0B46_002330"
     CDS             complement(6270..6533)
                     /locus_tag="I0B46_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAR2084587.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSGFTNIDDIDWEAL"
     gene            7008..7193
                     /locus_tag="I0B46_002331"
     CDS             7008..7193
                     /locus_tag="I0B46_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAR2084588.1"
                     /translation="MALNYGNKKWSLDEAEISTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(7335..9158)
                     /locus_tag="I0B46_002332"
     CDS             complement(7335..9158)
                     /locus_tag="I0B46_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAR2084589.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(9755..12460)
                     /locus_tag="I0B46_002333"
     CDS             complement(9755..12460)
                     /locus_tag="I0B46_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAR2084590.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPILQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
BASE COUNT         3651 a         2637 c         2389 g         3997 t
ORIGIN      
        1 ttcaaggtgt cttttctata tgttttggaa aataaaaaag atgctggtgt catccaccaa
       61 catcaaaaag tatagagctc tttttctttg tcataaaatc tttgtaaagc ttttttgtta
      121 ttcaaaacta gcggcttttc ataaccttat ttcacgaaca tcgcatctcc aaaactaaag
      181 aaacgatagc gctcttcaat cgcatgctgg taagcagata aagtcaattc tcttcctgca
      241 aaggcactaa ctaacatgac taaagtcgat tttggcaaat ggaaattagt tgagaaagct
      301 tctaccactt tgaattcata gccaggcgta atgaaaatat cagtccatcc gctgtctgct
      361 ttgatttctc cattaaattt cgttccaatc gtttctaatg ttcgaattga agttgttccg
      421 acagccacga ttttcccgcc atttttacgt acttggttca atcgctcagc tgcttcttcc
      481 gtcaaacggt agaattcact atgcatatgg tgttcttcaa ttttatctac gctgacaggt
      541 cggaatgtac ctaaccctac atgtagtgtc agatagacta attcaacacc ctttgcttcg
      601 atctcatcaa gtaattcttt cgtaaaatgc aatccagctg ttggcgctgc agcagaacca
      661 ttttctttcg catagacagt ttgatagcga tctgggtctt ccagacgttc tttgatatat
      721 ggaggcaatg gcatttctcc caatgattca agaatctcta gaaaaattcc ttcatactca
      781 aagtcaatga tccgtccgcc gtgttccaat tcttctttca ctaccgcttg caatctgcca
      841 tcaccaaaag agattcttgt cccaactttg gcacgtttcg caggttttat caatgtctcc
      901 cacgtatctc cttgtgtatt cgttagaaga aggacttcta aatgcgcacc agtatctggt
      961 ttttctccat ataaacgagc aggcagaact cgtgtattat tcataactag cgcatcacca
     1021 ggctgtaact cttcaatgat ttcatggaaa tgtttatctt ccatctctcc aattttccga
     1081 tccaaaacaa gtaaacgaga atgatcgcgg tcttttaatg gtgtttgtgc gatcaactct
     1141 tccggcaagt caaaatcaaa atcttctgtt gttaacatat cgtcactcac tttctatcat
     1201 gtgcttcatc attctatcat gtttttcatt ctgttggcaa aatcagcact ttttcacttc
     1261 tattttttgt tttcctaaat aaaaaaacta ccctatttgt attttttcat tgttttaggc
     1321 atactagtaa tagatagttc ttcattgaaa atagaaaaag aggtggcacc atgattgaat
     1381 ttcaaaatgt gtcaaaaatt tataaaggtg gaaaagtcgc tgtagaggac gtcaatcttt
     1441 catttgaaaa gggtgagttc atttgtttta tcggtacgag cggaagcgga aaaacgacga
     1501 cgatgcggat gatcaaccga atgactgatc caacacaagg gaaaattttg attgatggaa
     1561 aatccatcca agaaattgat ccagtggaat tgcgacgtca gatcggctat gttatccaaa
     1621 gtattggatt gatgcctcac atgacgatcc gtgaaaatat cacccttgtc caaaaattgt
     1681 tgaaagtcag tcaagaagaa cggaataaaa cagctgaaaa aatgattgat cttgtggaat
     1741 tgccccgtga gatgttagat cgctacccac atgaattatc tggtgggcag cagcagcgta
     1801 ttggagtcgt tcgtgccctt gcagcgaatc aagatatcat cttgatggac gagccattcg
     1861 gtgcgcttga tcccatcact cgtgattctt tgcaagacct tgtcaaagat ttacaagagc
     1921 gcttgggaaa aacgatcgta tttgtaaccc atgacatgga tgaagcgatt aagctagcaa
     1981 gccgtatcgc catcatgagt gaaggccgtg tgatccaatt tgatacacca gaaaatatct
     2041 tacgtcatcc tgcaaacgat ttcgtagaag agttgattgg agaagatcgc ctactacaag
     2101 ccaagccaaa tgcgacaaga gtcggagaag ttatgctaaa tacagcaatc acgattacac
     2161 cggaaaaatc attacaagaa gcaatcaaac tgatgaggga aaaacgtgta gatacgttgt
     2221 tagttaccga taattcgaat gtattaaaag gatttatcga tgtagaaacg ttgaataaaa
     2281 agcgaggaaa agtatccagt gtcggcgaca tattaaataa acatgttttt tacgtaaaag
     2341 aatccgccta tcttcgtgat accttgcaac gcattttaaa acgcggactg aaatacgtgc
     2401 cggtcgttga tgaacagaac aaagtcgtag gtatcctgac tcgtgcatca ttagtggata
     2461 tcgtctacga tgtgatttgg ggagaagatc ccgcaactac tgatgttgca gagtcagata
     2521 ctcctgaaga atctaaggag gtctgaccta tgcagcagtt ttttcatcag cacggttcag
     2581 aaattctctc aaaaggtttt gaacatcttt atatttcggg acttgctttg atcattggta
     2641 tcatcattgc tgtaccgctt ggtgtcttac tgacacgttt tccaaaagct gccacgattg
     2701 tgattggctt aaccagtgcc ttgcagacaa tcccttctct tgctttattg gcattgatga
     2761 tcccgctatt tggcgtaggt aaattacctg cgattatcgc actctttatt tattcattat
     2821 tgccgatttt acgtaatacg tacattggga tgaaaaatgt cgattggaat tatcgggatg
     2881 tggcaaaagg catggggatg actgagttcc aatccatttt ttccgttgaa ttgcccattg
     2941 ctatgccaac gatcatggct ggtattcgtt tagctgccgt ttacgtgatt gcatgggcta
     3001 cacttgcctc ttatatcggc gctggtggtt taggcgattt gatttttagt ggactgaata
     3061 attaccaacc agatctcatt ttggcaggta cgatccctgt aacgatttta gcactattgg
     3121 ctgattggct tctaggtctc ttggaacacc ggctgacacc gatcgcactt agagaggaga
     3181 atgaagaatg aaacgtatac taaaaatgac catggttctt ttctctcttc ttatacttgc
     3241 gagttgttct ttccctggtc ttgccagcaa tacagatgaa gacaccattt cgatcacagg
     3301 tggaatcact tcagaagcac agatcttagc aagcctagtt gccggaatgg ttgaacatta
     3361 tacagataaa aataccgcaa tcatcaacaa tctggctacc acaactatca accatcaagc
     3421 gatgatgaac ggagatgctt caatttcagc ggctcgctac acaggaacag atttaacgac
     3481 tacattgaac cttcctcctg aaaaagatcc gaaaaaagcg tttgctaccg tgaaagatga
     3541 atttgagaaa agatacgatc aaacgtggtt cccgtcctat ggttttgaaa atacgtatgt
     3601 ctttcttgtt cgaaaagaca ccgcacaaaa ataccattta tctaaagtca gcgatctgaa
     3661 aaatgtagcg gatgaattag tagctggtgt agatacttct tggatcaatc gtaaaggaga
     3721 cggttatgac ggcttccaag aaacttatgg cttctccttc aattcgatat tacctatgca
     3781 gattggactt gtttatgacg cagtcgaagc aggaaaaatg gatattgttc ttggctactc
     3841 tacggatgga cggatcgcca gttatgatct agtcatgttg gaagatgatc gtcaattctt
     3901 cccaccatat gatgctgctc cagttgtaaa caacaaaata ttagctgata cgcctcattt
     3961 aaaagaagcc ttaaataagt taggtcatac catcagtacg gaaaaaatgc aggaattgaa
     4021 ctacgaagca gataataatc tagtggaacc atcagtagta gcacaacgtt tcttacaaga
     4081 aaaccactat tttgaaggga agtgaagcaa ttggataaaa tgaatgtctt tcaacaattt
     4141 ttctattatt tccaggaaaa tggctcctat atctttgcac aatttatccg tcacttcctg
     4201 atttctatct atggtgtcct ctttgcagca gttgtcggga ttccggttgg gatcatgatt
     4261 tctaggagac gtaaattagc aaactgggtg attcgtttgg cgaatatcat ccagacgatc
     4321 ccttctctag ccatgatctc cattttaatc attggattag gcctgggagt caatgtcgtg
     4381 attgttactg tatttttgta ttccctctta ccgatcatca agaatacgta taccgggatg
     4441 atccaagtag acaaaaacat ccttgatgtc ggcaaaggca tggggatgac tgctagacaa
     4501 cgactattca tggtagagtt accactctct gtttctgtta tcatggcagg cataagaaac
     4561 gcgttagttg ttgccatcgg gataaccgcg atcggtgcat tcgtcggtgc tggtggtctt
     4621 ggagatatca ttatccgagg aaccaatgca acagatggca catcgattat tttagcaggt
     4681 gcattgccaa ctgcattaat ggtgattgtc acggactggc ttttaggtat tttggaaaga
     4741 cggctagatc cagccagccg tacatcacgc tgatgttatt gtaaaaaaca aaagcttctg
     4801 cgaccatttg ttggtcgcaa aggctttttt agttctgtca ttttttaagg caataatcca
     4861 ctctgattct tccctcctta tgggcaaaga gttgcttttc ttagctattt atccattccg
     4921 tatactccaa cttcatactg ccaacgggtt catctctgca tccaccgact aagttttttt
     4981 cttatccgct aaggcatttt gccaatccat tttcccatca ctcgtcatgg aattcatacc
     5041 acatgatctc taaatactcg tctgcttgtt ctttgaattg ggcatcaatt cttacatcct
     5101 ttggcgtggc tttcatttaa gccttttcca tcagtcctcg attgttcgat gccttcagct
     5161 acatcatcaa aactggtgac tgccgtatcg gtaacttcga tcatctttta tacaatcggg
     5221 aacttgccat cctcatcctt ggctaaaaat atatctagcc attccagctt ctcgccatgg
     5281 gaggtttgcg gttcctaaaa ccggtacatg attcttatgg actgcatccg tcgcaactag
     5341 acttggcggt acgatgatcc ttcaccagaa gagcaccccc gtaaaccaat tgatcgatat
     5401 attgccaata agaaaagaca ttggcgtaaa agatatcgat ccctcgtgat gcattgccgt
     5461 tgatactgct attcatggcc gaaagcgcca cgactttgac ttcttccgtt tgagtgacat
     5521 tcatgttcgg caatgtcgct ttggataccc gctctgctaa agaaacgaca ctggtattgt
     5581 atttcgtatc tgtatctttt ccaaaagacc atgccaacga atcctctggg aaccaataag
     5641 acgactccgg ttgattgtcc attgcttttt tcaacatact tttggctgtt tctcgataaa
     5701 cgacatttcc aattgtcttc gcctcacttt ggcgataatc gatggtcgtt gccactgcca
     5761 acactaacaa cgatgctcca attttactga ttttccttgt cacacttctt cctccttgtt
     5821 ttacataaac ccgtcaacat tccattccta aactcaaaca atgacccttc aaaggtggag
     5881 atataggaac aaattaaaga aatcatgcaa taaatccaag aaggttggtt ttagaacaaa
     5941 aaaagagtct cctttttggg gagactcttt gttaactatc taaaaaggtt ggctcttctc
     6001 acttcactca taatgtgtcc aggcagaata aatttccaca actttatttt cctcatcaac
     6061 tttgtaaacc actcgatgct gtctattcag cctgcgtgag tatctgcctt catgtgtagg
     6121 tctcagcttc tcaaaagact gcgtgggcat atatggatct tccttcaatg tctgaatcac
     6181 ttcttcaaat tgtttcttta aattcgattg cttgattttt cttaaatcca ctgtcgcaga
     6241 gtttttgatt gcgactgtat agttactcat caaagagctt cccaatcaat atcatcgata
     6301 ttcgtaaagc cactgtcgtc tttttcgcgg tctcgtactt tatctaaagt acctgtttgc
     6361 tcaagaaaaa gggtttcttg aatcgctctc cagtcttcta gcccaatcaa tacagcattg
     6421 tttccactac gatcactaat aatctcgatt tcagtatgat tttcatttac ctctttcaat
     6481 aactgataaa aatttttacg agctgtgcta ggattcatcg ttgtattagc cattgtctct
     6541 cctccttttt ctgatataat tataacgtac ttgattaagt acgtcaaaaa atacaataaa
     6601 gcacttaaat tcagcaaact tttcctcttt tttacataga gaaaaagacc cctttttagg
     6661 gaggatcttt gtccacgctc acttcttact cctttacgac tagcggtagc caaacgtaaa
     6721 gtaattggta acaacgtatc atctttagca aaggtcgaat ccaaagcctg tgagtctatg
     6781 caacaacacc aatacacgaa ggttcctcgt tgcagtgtga cttgattgat atcttgaata
     6841 acctgaggat acccacgaaa gatttgtaac ggttattcaa aactgatttt ggccgtcaag
     6901 tcttctactg cctcaaaaat gattgcctct ttcgtctcct gccattcatg ttcactatcg
     6961 atttgatact tgcttagacc gtcgggtttg tgaatcacca attaaaaatg gcactgaatt
     7021 atggtaacaa aaaatggtca cttgacgagg ctgagatttc tacttatgaa gacacatggc
     7081 acttaagagt cgaggtaaag gaagaagctg aaaatatagg aggctgtaca ttatttggca
     7141 aaggattcgg aaattatgat caagacatca aaatcaaaat gttttacagc taaataggaa
     7201 gaataataac atttcatacg ctgttcccat ctttaatggc ttatagaggg cacctttagc
     7261 tgggaataag acaaaacaga ccctctagct tcaattaaga gctagagggt ctgtttttga
     7321 ttttaaaact tggtttattt cttcaaatgg tagctatagg tgctgacaac gatattttca
     7381 cgccaagaca atcgcaatct taaccgcaaa cttgtttggt tatggagaat attctgccag
     7441 cgtctgtttg gtacaaattg aggaatcaac acagtagttg tatagttttg tttcgctgca
     7501 tttttactta cgatatctac ataacggata attggatttt caatcgagcg ataagaagag
     7561 tggacgattg aaaaacggac atctgggaaa tgctttttaa attctgcttc gatttctttt
     7621 tctttttcga catcttcgtc tagcgaaaca tgcatcgcaa cgacataatc accgatcgaa
     7681 cgtgcgtaat tcaacgcacc gatatttacc cgtgtcacat tcccgactaa tacaagaacg
     7741 gtattcccgt cataatcatg tagttttgca tcattttcta aacgaagctg ttctgctact
     7801 tttttataat gatcatggat cttataaaaa atgaacataa cgattggcat aatgatgaag
     7861 aaaggccaaa tgtcccctaa ccgatagacg aacaaaatcg caatgattgc atatgagatg
     7921 aacgcacctg taatattggc aatcgacttg cttaaccatc tttttccttc ttttttccat
     7981 ttgacgacca tccctgtttg agataaagca aatggaatga acacgccgat agagtataac
     8041 ggaatcaagc gttctgttga gccatggaaa ataaacagca atacgatcga acctagcgct
     8101 agtgtgataa tcccattgga atagcctagt cgatccccac gatcttgata catgtgaggc
     8161 atgaatttat cttttgccag attgtatgca agaactggaa aagcagaaaa accggtgtta
     8221 gcagcaactg ctaaaatcag cgctgtagca aattgaagga cgtaatacaa aataccatgt
     8281 ccaaagacag ctttaccaat ttgagacaaa acagtgactt ctttttcagg aacgatccca
     8341 taccaatagt tgataaatgt gatccccacg aagaagaaac ctaagatcaa tgccatcatc
     8401 gtcaatgtag cagcggcatt ttttgctctt ggcttcttaa agaatggtac agcattacta
     8461 atcgcctcca ccccagttaa agaggaagaa ccagaagaaa atgctcgtaa aatcaaagcg
     8521 atagaaacac ccggcacagc cgctcctgga agtgcagtag cttgcaatgg cacagcacct
     8581 gtcacgatat tatataaacc aacaacgatc agtaacgaga tcacaaggat aaatgtgtac
     8641 accggaaata ataggaacga tgcagattcc ctcagcccac gaagattcag catcatcagt
     8701 agcaaaacga ttgtcacaga aatagcgact tgatgaccgt agagtgctgg caccgctgat
     8761 gtgatcgctt cagcacctgc tgagacagat actgctactg tcaacatgta gtcgatcaat
     8821 aaagagccac cggaaatcaa gccagcattc tttccaagat tctcactgct gacgacatat
     8881 gctcccccgc catgaggata agcatggatg atttgacgat atgatagcgt taatgaaatc
     8941 aataaaataa taacaaaagc ggcaatgggt agcgaatacc agatggctgc agcagacaaa
     9001 gcgactaaaa cgacaacgat ttgttccgta ccatatgcaa tagatgataa cgcatcagac
     9061 gacaataatg ccaaagcagc aaatcgggtt aatttgtgct catcattttc agcagatttt
     9121 aaaggcttac ctactaacaa cctttttaag taatccaatt tctaaactcc tttacacaca
     9181 attttacttc aagttttata gtcgaaaatt tatagtaaac aagatgttat agtacgctca
     9241 ctgaccttaa aagtcaataa actttaagaa aataattgga aaaaaacaga aaaatatcaa
     9301 gcgctttttc aaacgcttta atacagcaaa aatctaacat ttttttatgg gctttttctt
     9361 tgaattctat aataaaagga tagaacttga cttagaaaat caagttctat cctttttgat
     9421 atcttaactt ttgaaagaga gatgtaaggt tgctttctac ccatcattct tactactcga
     9481 agcatagcgc agttgtcaca acctcttgat aaacggtgtt taaaagcttg catctgcggt
     9541 aataagccca accaaattaa aaacataaga agctgttttt atttggtcgt tcttattact
     9601 tgatgcagaa cagccttttc acaacctctt ggtacacggt gttcaatcag ctgaccctcg
     9661 gcattagctc aaaaaacggc aaaatatgag gaactatttt tccatttttt tctctaatac
     9721 tcaggtcaaa acgctgattt cacaacctct ttttttaatg aaaaatatgg ctctcatgcg
     9781 ccgcttcttt gaatttcgtc attttttctt cgatgaacgg atagattgcc gttcctacga
     9841 ttccaaagac aacaaacaga ccgatcatga tccagattgc tttcgtagca tttggccagt
     9901 aaatgcctcc tgcagattcc cgtaacaagt ttaccgcatg agtaaatggc aagaacggat
     9961 tgatcatttg gaagaatttc cctgagactt ggatcggata gttcccgcct ccgccagaga
    10021 tcgacagtac taagatgata atagcgatcc ctttaccgac attatcgaat aatgcagcca
    10081 agacatagac gatcatcatg aacgctaagg cgatcaagac agcaaatgac acgctgtaga
    10141 ccggattttg gacatctact cctagtaaga aaatattccc taatgtaacg atcaagcttt
    10201 gcgcaatcgc cattgtcaaa aatgtcagca tacgagcaac gaattgttct cgtttcgtaa
    10261 aattgtttcg gtctttcttt cctaaatagt attctgtcgt agcaacactt gaaagaagaa
    10321 cagcgccgac ccacagacat agtgcagtat aaaacggggt gctagctgaa ccgttatttg
    10381 cgattggata aagcgcattg gtttgtagct caacaggttg tgtaaagaag tcactttctt
    10441 ttgttgcatc tagtttcaat aatttgatga cttcgcctag atcgacttct tgttcgcctt
    10501 ttcggatcgc gtctgctgct ttttggatac cagctttgat atttggccag tcattgttga
    10561 tcaagtccgc agcagcgttc acagctgttt cgacttctgg cattttttca tttactgttg
    10621 tcaatgtacc ggtgatttca ttttttagtg ctggccaatc attttggata aaatcagcgg
    10681 ccaatcctaa tttttgctga aggatcggta attcattgtt gtataggtct gccccttcat
    10741 tgatgccatt gacgatggta tccatatttc cattcagcat tgtattggca tcatggattt
    10801 cttgtccaat tgcaggcatt tctttttgat atttttctaa aattgccact gcattcgtta
    10861 cagttgcttt cgttgaactg agaagcgaat caaagtcgat ctggtccgct ttgcttagcg
    10921 ccccttgcgc agtcccaatc gtatcgatga cttggtctaa tagagaagtg acagtggaac
    10981 tgatttgacc tgtattgata ccagcaatga tgccattgat attattaaca acggcatcaa
    11041 tttgatctag caattcatca atagccttcg tgtcttcatt ttgaacagct gtgttcaaag
    11101 cagataggcg gttttgcaga ttgcttagca agggttttaa ttgatttagc tgatcaataa
    11161 ttggctgcaa tttcgtattt ccagcacttg tttgcaattt agtcaggaac tcgaccagtt
    11221 tatcgattgc attttgttgt tgagatattc cgtcaataaa gccgttcaat acattgctga
    11281 tgtttttctt ttcatcatca gtcagcttac catcttcaat agctgttctt acagtatcta
    11341 ttaacgaacc tgtcgttgtt gccacttgtc caattgattt caatgtaaca tctacactgt
    11401 ttgtgatgct tggtatcgca tctttcagct gagaggcacc tgattttgtt tggtttgcta
    11461 gatcacttgc ctgattttct aaatttttca catctggcaa gatatcctgt acttgttgga
    11521 tgatcgtcaa gccttgtttt gcttcatcga ttccttggtt cattgtctct tggatcgaag
    11581 caaaatcgcc gtcgatctct gctagttgtt taccggcatt ttggatctct gggatttttt
    11641 cttgcaaatc aaggatgatt tttgcttgat ctttcaactc aggcattttg tcattcaatg
    11701 caacgacttt ttcacccatc tcatctactt tagggatata atccacgaat tcatttgctt
    11761 tattcagttt ttccttgatt tcaggtagtt gactttgtaa ttcgaggact tgttttgtgt
    11821 agccgtcgat cgtgtccaaa ttttcatctg ttgataaaat catatctttg actttattga
    11881 tacttactag gttcgtatcg atgtcgtaac ctatctcatt gaatactttc aataaagtcg
    11941 aactggctgt tttgataaat tcatttgtga tttgcgattg gatcgaactt gctcctttat
    12001 cggtgatttt cggtgcaatc gcattgattt tttcatttac cgtatattcg atttttggtt
    12061 tcttgatatc accactagta aaactcagca gatcttctga gaaatccttt ggcaagtaaa
    12121 tacctgcgta atactttccg gaacggactc cgtcttctaa ttgttctttt gaatcaacga
    12181 attgccatcc taattgttta tttttatgaa gcgattcgat gacttgctcc ccaatggcca
    12241 cttctttacc ttgaaactct gctggcttgt ctgcactgta aacagcaatt ggcagctctc
    12301 cagtatttcc atatggatcc cataatgctt tgatgttaaa ccacgcatat agcgaaggga
    12361 tgatcatgat cgcgatgatc agaagtgttg caatcgggtt tttgaaaatc cgtttccagt
    12421 caagcaaata taatcgaagc gtatttttga ttgactgcat agttctcccc cttcttcttt
    12481 tttcagctga attactttac catgtatttc atactttttt caatcggctt ttttcaaaaa
    12541 agtcgaattt ttggacatat cacggcaatt gtctcattgt aatctcttac atattgagaa
    12601 ataattaagg attacagact ttactttatc atgaaataaa agggaaagat aattttatgc
    12661 caaagttttt atct
//
LOCUS       DACKAC010000078        12458 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.079, whole genome shotgun sequence.
ACCESSION   DACKAC010000078 DACKAC010000000
VERSION     DACKAC010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.079"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(180..737)
                     /gene="frr"
                     /locus_tag="I0B46_002334"
     CDS             complement(180..737)
                     /gene="frr"
                     /locus_tag="I0B46_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAR2084591.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(740..1462)
                     /locus_tag="I0B46_002335"
     CDS             complement(740..1462)
                     /locus_tag="I0B46_002335"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAR2084592.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1598..2479)
                     /locus_tag="I0B46_002336"
     CDS             complement(1598..2479)
                     /locus_tag="I0B46_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAR2084593.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2578..3360)
                     /gene="rpsB"
                     /locus_tag="I0B46_002337"
     CDS             complement(2578..3360)
                     /gene="rpsB"
                     /locus_tag="I0B46_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAR2084594.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3719..4198
                     /locus_tag="I0B46_002338"
     CDS             3719..4198
                     /locus_tag="I0B46_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAR2084595.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4415..5506
                     /gene="phnW"
                     /locus_tag="I0B46_002339"
     CDS             4415..5506
                     /gene="phnW"
                     /locus_tag="I0B46_002339"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAR2084596.1"
                     /translation="MYKLLTPGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSGSFGVESVLSSVIRPEDTVLICSNGAYGQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQEKDLL
                     FIVDAMSSFGGIPIRVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5499..6284
                     /locus_tag="I0B46_002340"
     CDS             5499..6284
                     /locus_tag="I0B46_002340"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAR2084597.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(6630..8321)
                     /locus_tag="I0B46_002341"
     CDS             complement(6630..8321)
                     /locus_tag="I0B46_002341"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAR2084598.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8743..9690)
                     /gene="arcC"
                     /locus_tag="I0B46_002342"
     CDS             complement(8743..9690)
                     /gene="arcC"
                     /locus_tag="I0B46_002342"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAR2084599.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9805..10824)
                     /gene="argF"
                     /locus_tag="I0B46_002343"
     CDS             complement(9805..10824)
                     /gene="argF"
                     /locus_tag="I0B46_002343"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAR2084600.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10915..12144)
                     /gene="arcA"
                     /locus_tag="I0B46_002344"
     CDS             complement(10915..12144)
                     /gene="arcA"
                     /locus_tag="I0B46_002344"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAR2084601.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3443 a         2565 c         2211 g         4239 t
ORIGIN      
        1 acttaaaaga ttcccactat cttaagtatt atagcaatat ctaactgttt ttcctatgaa
       61 atcattgttt ttttatttaa aaactgaata attttttctt tttttaattt ttaaataaaa
      121 gaaatataaa taaaaacaga ggaagcggaa tgatccgccc ctctgttttt atgattttat
      181 taaacttcca ataattcttt ttctttatca gcagcgatct tatctacttc tttaatgctg
      241 ttatctgtca atgtttgtac gtctttttct aatccgcgca aatcatcttc ggtaatatca
      301 ccattttttt gttgtttttt atattcatcg atcgcatcac gacggatgtt acgaatcgcg
      361 atttttgcat tttctgcttc ttttttcaca tctttagcta attcttttct gcgttcttct
      421 gtcagttgcg ggataactaa gcgaatgaca tttccatcat tggttggact gattccgata
      481 tcacttgcca taatagcttt ttcaacatct tgaagaatgc tcttgtcaaa aggtgtgatc
      541 attaaaacac gtgcttctgg aatttggatt gatgccattt ggttcaatgg tgttggcgca
      601 ccgtagtagt tcacagtaat acggtccaaa aggctagcgt tcgcacggcc tgcgcggatt
      661 tgcccaagtt cacgttgaag attttgtgct gcttttttca ttttttcttt tgcttctgtc
      721 ataattgcat ctgccatttt tatttccccc ttacagttgt tccgatattt tcgcctaaga
      781 ttgcacggcg aatatttccg tgttcattta aattgaagac aagcagtgga atatcattat
      841 ccatgcttag tgaacttgca gttgaatcca tgacttgtaa tcctttggca ataacttcca
      901 gatgagtcag ttcttcaaat tttactgcat ttgcatctac tttcggatca gcagaataaa
      961 caccgtctac attgttttta gccatcagga taacatctgc gccgatttct gccgctcgta
     1021 atgctgctgt tgtgtctgtc gagaagtacg ggttgccggt accgcctgca aagataacga
     1081 tccgtccttt ttctaaatga cgttctgctt tacggcgaat atatggctct gcgatttgac
     1141 gcatttcaat cgaagtttgc acgcgtgtcg gtacacccac gttttccaac gtatcctgta
     1201 aagctaaggc attcataact gtagctaaca tacccatgta atcagcttga gcacgttcca
     1261 tgcccatttg tgctccgatt tgtccgcgcc agatatttcc gccacccaca acgattgcca
     1321 tttcaacgcc cagctcgtga acttctttga tttcttggat gatttctttg attactggtg
     1381 gtttgattcc aaatcctgca tccccagcta aagcttctcc actcaatttt aagactacac
     1441 gctgatattt cggtgtgacc atgttcattc ctccaatatt ttctattgcc attttaccat
     1501 acttagatga tttcgacact ctaagactgg tttttcttgt tttgcttaaa aaaagggagc
     1561 gcacaaaccg tgcgttcccg tctgttattc aacagaatta ttttttcact tggctcataa
     1621 cttcttctac aaagttatct tcgcgttttt cgataccttc gccaacttcg aaacgtacga
     1681 atgttttaac agttgcgcct tttgatgcaa cgtatttttc aacagtcata tcaggatctt
     1741 taacgaatgg ttggtccacc aaagcgattt ctgctttgaa tttcttcaag cgtccttcta
     1801 ccattttttc aacgatattc gctggtttgc cttcgttcaa tgcttgttct gtaagaacag
     1861 ttttttcatg ttctaattca gcttctggaa tttgtgattc gtttacataa cgaggattga
     1921 tagctgctac gtgcatagct acgtctcttg caactgtttc gtcagttgtt ccatctagaa
     1981 cagtcaatac ggcaatacgt ccgcccatat gcaagtatcc accaaatgca gcattgtcgt
     2041 ctttttctac aacttcaaag cgacggaagc tgattttttc tccgataact tgagttgctt
     2101 cgatcaagtc agattcaatt gtacctttat ctgttttgat tttcattgca gcttccatgt
     2161 ctgctggttt gttttcagca acaagttctg caatttcttt tactaaatct tggaacattt
     2221 cgtttttaga aacaaagtct gtttctgagt ttacttcaac gattgctgca gtgttacctt
     2281 tcacagcaac tgaagccaat ccttcagctg caatgcggtc atttttcttc gcagcctttg
     2341 ccattccttt ttcacgtaaa agatcaactg ctttttccat gtcgccttct acttcaacta
     2401 atgctttttt cgcatccatc attcctacgc cagtcatttc gcgtagttct tttaccattt
     2461 tagctgtaac gtctgccatg tttaactcct cctgtgtgtt tttcttttta aaaaaagctg
     2521 cttcaaagga gagtgaggct tgtcgcccag cctttgaaac agctccatca ttcaaaatta
     2581 ttctgctgaa gaattgtcgc cttcaacaac atctacgatt tcttcgattg atgttgctgt
     2641 gttttcttct gtgaaatctt cttcaactac ttgatcttca ccttggtttc cttcgatgaa
     2701 agcatcagcc atttttgaag taatcaattt aacggcacga attgcatcgt cgtttgaagg
     2761 gattactacg tcgatttcat ctggatcgca gtttgtatca accatagcta cgattgggat
     2821 atttaatttg tgtgcttctt gaacagcaat acgttcttta cgtgggtcaa cgatgtacat
     2881 tacatctggg attcttggca tatcagcgat accgcctaag aatttttcaa gacgttcacg
     2941 ttctttgttc aaaccagcaa cttctttttt aggtagaact tcaaaagttc catcttcttc
     3001 cattgcattg atttgtttca aacggctgat acgtttttgg atagtatccc agtttgtcaa
     3061 tgttccgcct aaccaacgat ggttaacgaa gtattgtcct gcacgagttg cttcttcttt
     3121 gattgcttct tgtgcttgtt tttttgtacc tacgaataat gctacgccgc cttcttcagc
     3181 aacgctcttc atgtaatcat aagctgcatc tactaattta actgtttttt gcaagtcaat
     3241 gatgtagatt ccgtttcttt ctgtgaagat atatttcttc atttttggat tccagcgacg
     3301 agtttggtga ccaaagtgta cgccggcttc tagtaattgt ttcatagaaa ttactgccat
     3361 gtttgtttcc tccgatttgg ttttattttt cctcttctta tcttctacct tgccggcaaa
     3421 ctttttcaag cacctaccga caatccaata agaatgtgga tttgctgatg cataaagcac
     3481 cctcagctat tatacagaca aaagatccgt atcgcaagag caaatatact tttctgctca
     3541 acattcaata gtttacctga aagataaaaa gaattcaaga aaaacagtag atttctcttt
     3601 gtttctttat tatccttttt ccataaaata attttaggta taaaagacga atattattgg
     3661 gcaatccttc ttttttttgt tatgatacat gtgaaatctt tcgtaaagga gaaagaaaat
     3721 gcgcaaagct caaagacatt tgctgattaa acaagtcatt gaagaattcc cgatccgtac
     3781 gcaagaagaa ctattaaaca aacttgggca ttatggcgtt accgccacgc aggcaacgat
     3841 ttctcgagat atccgtgatc taaaaatcgt gaaggcacct gatgaaaatg gcgtttcccg
     3901 cttcgtattg tttcgcgaaa aaggaactcc tgagactagg actgaggaag aggatcggct
     3961 ggttcaaatg attgaagaca ttgtattgaa agtcgaacga gtccactttt tgacgatcgt
     4021 caataccttg ccagacaatg ctcatttatt tgcatctgtt ttagatgaga tcaaacctcc
     4081 ctctattgtc agtacaattg ctggattcga tacgatcatg gtcatctcgg aaaatgagga
     4141 ggacgcaaaa cgagtagaag aatttcttca ccatccaact gaagtatcac ttttttaatc
     4201 agagcacgta ttcctaatat ggaatgcgtg tttttttatt ttcaatattt tccttattaa
     4261 ataaaccttt acactcttta caaaaataca tcatttttta ttcaattttt tcaacacctt
     4321 gattgaatca caataatcat catttatatt ggatgttaac aagagcagct tcttgctctt
     4381 tgtgtatttc aaaaaagaaa tgaaaggaaa tcatatgtat aaattattaa ctcctggacc
     4441 attgacgact tctgaaacag tgaaaaatgc tatgctgatc gatcattgta cttgggatca
     4501 agaatacaaa gaaattacac aatggataag aaaaaaatta ttggatcttg ctcaagtatc
     4561 ggaaggaata tacacaacgg tcttaatgca aggtagcggc tcctttgggg tagaatcggt
     4621 attaagttct gtgatcaggc cagaagatac tgtactgatt tgttcaaacg gcgcatatgg
     4681 acaacgaatg atcaacatgt gcgaatgtct gaaactgaac tataccgttt atgaggtatc
     4741 ggaacatcaa atcctccaag ctcatgtgat tacacagctg attgaagaga atccctctat
     4801 ctccgcacta gcaatggtcc atagcgagac tacttccggt attttgaata atttggaaga
     4861 gattgctgct gtcacacaag aaaaagatct tttgtttata gtggacgcga tgtcaagctt
     4921 tggtgggata ccgattcgtg tagccgaact agggattgat tttttgattt ccagctcaaa
     4981 caaatgtatt caaggtgttc caggcttttc ttttgtactt gctaataaag cggaactaga
     5041 aaagcgaaaa aatgtgagtc gaagtttgtc acttgatcta tatgaccagt accgcgtgat
     5101 ggaaaacgag aatggcaaat ggcgtttcac ttcccctacc catacggttt tggcttttca
     5161 acaagcgctg aaggaactag aggcagaagg aggaatcgaa actcgatatt cacgttatct
     5221 cgccaataat catgtactac gacaaaaaat gacagagatg gggttccagc ctttcattca
     5281 agaatatcag gggccattca tcacctcttt tctttaccct aaggactggc gatttgattt
     5341 taatcatttt tatcagtatt taaagcaaaa cggatttgtg atctatcccg ggaaaatcag
     5401 cgatcatgaa tgttttcgta tcggaaatat tggcgagatc tatccggaag atattttgga
     5461 actgacagca attatcaaag actataagga gaacgttaat gattaaaact gtaatttttg
     5521 attgggcagg aacaactgtg gactttggct gtatggctcc tgtccacgct tttagaaatg
     5581 cttttctaga aaaaggaatc caactgactg acaaagaaat ccgcgaaccg atgggaaaat
     5641 taaaatggga tcatatccaa cagcttcttt ccctcccttc tgtcaaaaaa caatggatag
     5701 ctatttatgg acaactacct caggaaaaag aggtggagga attgtatcaa caatttgaac
     5761 attatctgtt taaagatttg gtcaagcatt ctaccttgaa acccgacaca ttggaaacga
     5821 tcgaacggtt gagaaacgct gggatcaata ttggttccac gactggattt actgctccta
     5881 tgatgacaat cgtagcagaa accgcaaaaa aagaagggta ttcaccagac ttcatggcaa
     5941 cttctgaaga cgtcgaaggg tatggtcgac cgtttcctta tatgatccat aaaaacatgc
     6001 agtacttcaa aaacaagtcg gttgatgaag taatcaaagt cggagatacg gtctcagata
     6061 tccaggaagg gaaaaatgcc ggagtgttga cagtaggtgt aattgaaggt agttcgctaa
     6121 tggggatttc ttatgatgag taccaaaaat tagagatggg gaaaaaagat cgtttaaaac
     6181 agtctgttgc tcttagattc caacaagctg gcgctgatat ttgtgtaaac aatttaaaag
     6241 agttaacaga atacattttg tacgctgaca agaatccaga atgactttta tacaaaatca
     6301 gaggtcgtga aaaagctgta ctgcatcaag taataagaac agccaaacaa aaatagctcc
     6361 tcatattttt cgtttggtta ggcttattac cgcagatgca agcttttgaa caccgtttat
     6421 tcgaaaaaaa aagggagctg tgacacgact tttgtcacag tccccttatt tacaaacaaa
     6481 cagtgtttaa gagacaacct ctccactaaa cggtgttcat tcagctgact ctcggtattt
     6541 gctcaaaaaa cgataaaatt tgaaaagcta ttttctcttt tgtttctcta atactcggat
     6601 cagaacgctg atttcacatt ctctttttct tacatcttat ctggtgcatg aagtcctagc
     6661 aaacgaaggt cttctttcaa taatacagtc actgcataaa ctagagctaa gcgtgcttct
     6721 ttttgttcat catcagctaa aatcttcgta tgagcatagt atttgttgaa tgcttgtgct
     6781 aatttgattg catgtttcgc aatcactgac ggttcgtatt tttccccagc agacaagacc
     6841 gtttcagggt atttttgaac taatttaacc acttcccagc tggtatcgtc attcaatgca
     6901 tagtctgctg tttctgaagg gacaaatcct gctttttcca acaggctgac tgcacgagca
     6961 tgtgtatatt gcacataagg tccagtttct ccttcaaatc gaacaacttc ttccaggtta
     7021 aagtcaaatg tattcagtcg atcgtttttc aaatcgtgga aaatgactgc cccaacccca
     7081 acttgtttag ctacagcatc tttgttttcc aaatcagggt ttttctcgct gatttgttct
     7141 ttagcagatt ggattgcttc atttaatact tcttctaata agacgatctt tcctttacga
     7201 gttgataatt ttttaccacc ttgcgtgatc aaaccaaatg gaatatggcg catatcgtca
     7261 gaccagtcaa agcccatttc cttcaagaca gctttcaact gtttaaaatg atagctttgt
     7321 tcattcccta ctacatatag agattgtttg aagtcgtaag tacgtttacg gtacaatgca
     7381 gcagctaagt cacgtgtaat gtatagtgtc gcaccgtctg acttcttaat caacgctgga
     7441 ttcaggtcat aagcagatag gtcaacgatc tctgcacctt tatcctcgtt cagcaaatgt
     7501 ttttcttcta aaagtttgac gatttcatcc atcttgtcat tgtagaaagc ttcaccgttc
     7561 aacgaatcga aacgtacttc cagcagatca tagattttat tgaattcttt catcgattca
     7621 tcacggaacc attgccataa ttgaatagcc tcttcatcgc cttcttccag acgtttgaac
     7681 caagcacgag cttcttcatt taattctggt tctgtttcag ctacttcatg gaattggaca
     7741 tataaacgaa gcaattcatt gattggttct gctttaacag cttcttctgt accccatttt
     7801 ttgtaagcga cgatcagttt tccaaactgt gttccccagt cacctaaatg gttgatgcgg
     7861 attggctgat aaccgatttt ttccataatg aaaccgatcg aattaccgat aactgtcgaa
     7921 cgcaaatgtc ccattgagat tggtttggca atattaggag aagacatgtc gattggtact
     7981 gttccttgat tcccgatatt gctatcccca taatgttctt tttctttcac tactgtttgc
     8041 aaaacttttt tgctgataag ttctttattc atgaaaaagt tcaagtaagg tccgacaact
     8101 tcgatttttt caaagttggc agaatcgatt ttttctgcta aatctgcagc gatttgctgt
     8161 ggcgcttttc ggtaaacttt tgctaatgaa aaggctggaa atgcaacatc tccgtgttca
     8221 gctgattttg gattttctag taattgttcg acttgttcca gtgttaaatc atctttgaca
     8281 gcgtcataga cagcttttgc gacaagatct ttattattca tctttattcc tcctaaagtg
     8341 ctcccttgat tgttaaacaa aaaaaatctc tagcgacggc atacttagta tgccatcact
     8401 agagacgaga tttcccgcgg taccactcca attgtcctaa aaaaggacca gctccatctg
     8461 atatcggtag acgaccgtct gtttaaaaca gaattctcca agtgcgcttc gttgtatctt
     8521 cttcattcgg cttccaccac ttccgaattc gctagccaag aatgatacaa gtactctctt
     8581 gttcaacaaa tcttagtctt attaaacttt cttgaattat atcagaaaaa aaaacgaaag
     8641 gctagtcgtt tctgataaaa aaacaatcca gatggcctaa aggagaagaa ataccacctg
     8701 gatagctata aatcatttcg taaatacgaa agacttaaca aattaatcag ctacgataat
     8761 cgttccttct tcagaagcta aaagattctc gatattttct aaagaagtga tgattgcctt
     8821 agcgtttggt tttgcttcaa caaatgcaat agctgcctct actttcggaa gcatgcttcc
     8881 tggagcaaat tgcttttcat caatgtattg tttcatttct gaaacagtaa cagtttctaa
     8941 tttcttttga tctggttttt ggtaattgac atacacattg tctactcctg tcaatacgat
     9001 cagcaaatcc gcatcgatga tttcggctaa tttttctgaa gcaaaatctt tatcgattac
     9061 ggcttctcgt ccttctaatc cagtagctgt ttctacaaca ggaatcccac cgccaccaac
     9121 agagactgtg ataactcctt gatcgaccag cgtttcgatg acacgtgctt ctttaatcga
     9181 gatgggttta ggggaagcga ctacttttct ccatccacgt ccagcatctt ctttgaatgt
     9241 cacagtggag tcagcattca tttgttcttt ggcttcttct tccgtgtaga atggaccgac
     9301 tggtttactt ggatttttga atgaaggatc attttcatcc acaataactt gagtgactaa
     9361 agaaaccaca tctttatcga tccctttttc cttcagtact tcgcccatgg cattttgtaa
     9421 ccagtaaccg atagatcctt ctgtcattgc aacacatgta tctaaaggca ttgccggtgt
     9481 tttctcagaa tcagcagctt gctgctgaat caataggtta ccgacttgcg gaccatttcc
     9541 gtgggagatg atcaattcat ctccttgttc gataaatttc actaaatact ttgctgtttc
     9601 cattaacgct tcttgttggg cttttgcact tgcatccgta gacaagattg cattaccccc
     9661 taaagcgaca actacttttc gtttggccat ttcattcgtc ctttcttcag ttctaccgaa
     9721 ttttctagat aacaaaacga gccagcgttt tgtgattttc cctcaatgct gactcgtttt
     9781 ttcgtgagat gaatgtggtt atgcttaaac gcgagggata aacaagttac ctaatgttgc
     9841 tgccatgatc gctttgattg agtgcatacg attttctgct tggtcaaatt gacgagcata
     9901 tttgctgcgg aagacttcat ctgttacttc catttcagtg atgccaaagc gttctttcat
     9961 ttgttcaccg taaacagtgt ttgtatcatg gaaagctggc aagcagtgca agaagatcaa
    10021 acgatctgtg ttgtgtgttt tttctaccat ttccatattg atttggtaag gtttcaacaa
    10081 tttgatacgt tcttcgaatt tatcttcttc acccattgat acccatacat cagaataaag
    10141 gacgtctgct ccgtctacac ctttatcaac atcgtcggta atcatcaatt gtgcaccgga
    10201 tttttcagca aagccttctg ccattttgac gatttcttct tcaggttgta attcttttgg
    10261 tgcaacgata cgcatattcg ctcctaaaat tgcacctgtg actaataaag agttagccat
    10321 attgtttcga ccatctccgc agtaagcaac agtgatacct tctactgttc caaagttttc
    10381 ttggatagtt aaaaagtcag cgatcatttg tgttggatgc cattcatcag tcaagccatt
    10441 ccaaactgga acaccagaga attctgctag ttcctctacc attttttgac tgaatccacg
    10501 gaattcgatt ccatcaaaca tacgacctag tactttagct gtatcttctg tggattcttt
    10561 tttacctaat tggatatcat ttgcacctag atattcagga tgtgcaccta gatcgatcgc
    10621 tgcagtagta aatgcagaac gagtacgtgt agatgttttt tcaaataaaa gcgcaatatt
    10681 ttttccttct agatagtgat gtgggatacc acgttttttc aagtctttca agtgttctga
    10741 aaaatcgatc aaatattgta attcttcttt tgtaaagtct ttttctgcta ataagcttct
    10801 tccttggaat actgattctt tcatattaaa cattctcctt tagattgttt ttatttaaga
    10861 ttaaagagga aatcccgacg gcagctgaca gtaagaccgc cgtcaggaaa caaattactt
    10921 caagtcttca cggacaagtg gttggctcat gcagcgtgga ccaccacggc ctcgtgacaa
    10981 ttcacttgaa ttgatttcta acactttgat gccatagcta cgaagcaatt cgttcgatac
    11041 ataatttcgg ttataagtga ctacaacacc tggagcgatt gccaatgtgt ttgatccatc
    11101 attccattgt tctcgaggtg caacgatctc atctccatta ccagttggga tcagtacaag
    11161 atcatctaat ccaagagcag ctttcatcgt ttcgtgtaag ttatctgaat gagtaatctt
    11221 gatatcatca ccatctggtt cgattgtaaa gacatccatc ttaccgtctt tactttgaat
    11281 tgctggatgg atagtgaatt tatcgtgatc taccattgta aatacggtat ccaaatgcat
    11341 cattgcacgg ttgtttggaa ttttgattgc taacactttt tcaaatcctg aattttttgc
    11401 gaataggtgg cgtgctaatt tttctaaagc tttcgcatta gttcgttgag aaatcccaac
    11461 agctactact ttatcgctta agatcaattc gtctccgcct tcgatatgat ctggattttc
    11521 acgatccaac catacttcga ttcctttatt tgcaaatctt ggatgatgtt tcaaaatata
    11581 ttctgtaaac atcgattcac gacgacgtgc ttcaaaagtc atcttattga ctgtcatccc
    11641 atttcccatt gatgcggaag ggtcgcgtgt aaagtacaag tttggcattg ggtccatgta
    11701 aaatggatag tcatcgtctg ctgacagatc gtatagactg ctagaacgta cttcaatatc
    11761 ttttgtacga accccagcca tgattttatc taccatttct tgcgtttcca ttgatagaag
    11821 gaactcatga agaccctctc ttacagcatt cgatgcaata tgagattcat ccaacatttt
    11881 attcaagaat tgttctttga cattgcctgc gtcaatcgct tctgccgcta atttttccaa
    11941 gtaaagagtc tcgacgcctt cattttgcaa tgtctttgca aagttgtcat gttcttcttg
    12001 agcaatcgga agatatggaa tatcatcgaa caacagacga tccataatat ctggtgtcaa
    12061 attttccact tcttgtccag gccttttcag taaaactgtc tttaatttcc ctatttcaga
    12121 gaaaacgtga ataggtttat ccatgttgtg ttgcctcctt ctttttgatt acatgtatag
    12181 gatatcctcc ttttgtaatc actttcataa ttttgtctca ttcaaaatga gacattcctc
    12241 acattaatat tcgataatta aataattaaa cgtttttcta tttttaagcg taatttattc
    12301 attttttatt ctgtatacac tttcaaaaaa atatttattc attgaaaaaa attttttgaa
    12361 taaattaaaa aaataaatta tattagcgtt gttagcgttt actatatgta ttattgttgt
    12421 tttttataca caaatcattc gcttcaaaac aaattatt
//
LOCUS       DACKAC010000079        12213 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.080, whole genome shotgun sequence.
ACCESSION   DACKAC010000079 DACKAC010000000
VERSION     DACKAC010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.080"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..292)
                     /locus_tag="I0B46_002345"
     CDS             complement(<1..292)
                     /locus_tag="I0B46_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00374 family protein"
                     /protein_id="HAR2084602.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEF"
     gene            433..1239
                     /gene="proC"
                     /locus_tag="I0B46_002346"
     CDS             433..1239
                     /gene="proC"
                     /locus_tag="I0B46_002346"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAR2084603.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(1318..2874)
                     /gene="rny"
                     /locus_tag="I0B46_002347"
     CDS             complement(1318..2874)
                     /gene="rny"
                     /locus_tag="I0B46_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAR2084604.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(3285..4334)
                     /gene="recA"
                     /locus_tag="I0B46_002348"
     CDS             complement(3285..4334)
                     /gene="recA"
                     /locus_tag="I0B46_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAR2084605.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(4443..5684)
                     /locus_tag="I0B46_002349"
     CDS             complement(4443..5684)
                     /locus_tag="I0B46_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAR2084606.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(5924..6502)
                     /gene="pgsA"
                     /locus_tag="I0B46_002350"
     CDS             complement(5924..6502)
                     /gene="pgsA"
                     /locus_tag="I0B46_002350"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAR2084607.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(6711..7595)
                     /locus_tag="I0B46_002351"
     CDS             complement(6711..7595)
                     /locus_tag="I0B46_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAR2084608.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            complement(8373..9659)
                     /locus_tag="I0B46_002352"
     CDS             complement(8373..9659)
                     /locus_tag="I0B46_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAR2084609.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(9652..10920)
                     /locus_tag="I0B46_002353"
     CDS             complement(9652..10920)
                     /locus_tag="I0B46_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAR2084610.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            11172..11633
                     /gene="mscL"
                     /locus_tag="I0B46_002354"
     CDS             11172..11633
                     /gene="mscL"
                     /locus_tag="I0B46_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAR2084611.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(11692..12138)
                     /locus_tag="I0B46_002355"
     CDS             complement(11692..12138)
                     /locus_tag="I0B46_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAR2084612.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
BASE COUNT         3365 a         2639 c         2182 g         4027 t
ORIGIN      
        1 gaaactcttt tctttccatt tttggttttc cttgtttttc taagactctg atcgtcaccc
       61 aatcatacaa caacatcgga gtgacaccca gcagtccagc cagaatcatg agcaggatcg
      121 tcatgcggct ttgttgtccc atcctttgcc aaatatcttg ccaactcatg ccattagcaa
      181 tatttgcgac ttgatttgcc acaaagacta acacggagcc gaaaaaaatc agttttaaaa
      241 tcagactatg ttcctttatc cattgcagca tcaagtatcg tttatttttc acattttccc
      301 attcctaact tgtatacttt tgtgtcatac cccaatgtta tcttatttat tttaattttt
      361 ccactttaga cctgttcttt gcttgcgatg atagaaaaag aaagatatcc taatgctaaa
      421 ggagaggata acatgaaagt aggatttatc ggcacaggga atatggcaca agctattatt
      481 gatggtttat taaaaaaagg aacagtctcg aaagaagagg tgttcgtttc cagtggacat
      541 tttgaaaatg cacaaaaatt tgctgctgaa actggcgtcc atgcatgcaa aagtaatcag
      601 gaagttgcat cacaatcaga tatcgttctt ttagccgtga aaccaaaaat cattgaaaaa
      661 gtattggaag aactaagaga atatagccag aaagtcttat tcatttctat tgcttcagga
      721 aaatcattaa cagatttaga aacagcgctt gaagatcctc aggcctccat cgtacgtgtg
      781 atgccgaatg tcaatgtcag cgtaggagca ggcgtttctg cgatttgcaa aaatgaagct
      841 gccacggagg aacagcttca gcaagctacg atgatttttg aagcaatcgg cagcgtctat
      901 cctttagcag aatcagattt cagtacattc attggccttg ctggcagctc acccgcattc
      961 gtttacatgc tgatcgatgc gatgggacgg actggtgtac tgaatggttt gtccaaacag
     1021 acagccactg agattgctgc aaaagcaatt atgggttctt gtcaaaaatt cctttcaagt
     1081 gataaaaatc catgggaact tgtagaccaa gtttcttctc ctggcggtac aactgttgca
     1141 ggtgtagttg ccttagaaga agcaggattc atcccagcaa tcatcaaagg aatcaatacg
     1201 acgatcgaga aagatcttga gatgttgaat aaaaaataat gatagcaatg ttttttcgta
     1261 ccttacaaaa aaagaagagc tgtggctttt gccgcggctc ttcttttgta ttttttatta
     1321 tttagcataa tcagttgcac gagtttcccg aataacgatt actttgatgt gtccaggata
     1381 atccagttct tcttcgattc gtttacgaat atcacgtact aaacgaacag cgtctaaatc
     1441 cgagatttct tctggcttga ccattacccg aacttcgcgt ccagcttgaa ctgcgtaact
     1501 tgattctaca ccggcgaagc tgttcgaaat attttccaga cttcttaaac gacggatgta
     1561 gttttctaat gattcacttc gcgcaccagg tcttgctgct gagagcgcat ctgctgcagc
     1621 tacaaggaca gagataacag aagtagcttc cacatctcca tgatgagaag cgatcgcgtt
     1681 gattactact gggttttctt tgtacttggc agctaattcc gcaccgatct caacgtgaga
     1741 tccttcaatc tcatggtcaa gtgctttccc aatatcatgg agcaacccgg cacgttttgc
     1801 taattggata tcttcgccaa gctctgctgc taagatacca gctaattttg caacctcgac
     1861 cgaatgcttc aagacgtttt gtccataact agttctgaat cgcagacgtc cgatgatctt
     1921 gattagatct ggatgaagtg tatgagcacc gacttcaaac gctgcctctt ccccgtattc
     1981 gcgaatacgt tcatccattt ctttacgaga tttctcgacc atttcttcga tacgcgctgg
     2041 atggattcgt ccatcttgta tcagtttttc caaggtcatc cgtgcgattt ctcttcggat
     2101 tggatcgaat ccggaaagaa caactgcttc cggtgtatca tcgatgatta aatcgatacc
     2161 agtcagcgtc tcaagcgttc gaatattacg accttctcgt ccaatgatac gacctttcat
     2221 ttcatcattc ggaagggtaa cgactgaaac agtcgtttct gaaacttgat ctgctgcgca
     2281 gcgttgaatc gctaatgata ataagttttt cgctttgcga tctgcttctt ctttggcacg
     2341 ctgttcagat tcttttacca ttagagttaa ctcatgattc agctcttctt cagtcgattt
     2401 catgatgaca tctcgtgcgt cctcttttga taaaccagca attttttcta gttctttttg
     2461 ttgatcttca atcatttttt caacgtcttt ttctcgctct tcaattaatt gctgttttga
     2521 actaagtgct tcttctttac tttcaagtga gttctcgcgt ttttctaacg agtcatcttt
     2581 acgattcagc gtttgttcgc gttgaatcaa acgattctct tgagatttta attctaactt
     2641 actttctctc agctcacttt caacttcaga acggtatttc tgattttctt ccttagcttc
     2701 taacaatgct tcttttttta gagtttctgc ttcttttttt gcactatcga tgattccagc
     2761 agctgatgat tgtgcaccag ctatttcttt ctcgtgacgt gactttgcga ccataaaccc
     2821 taaaccaaga ccgacaatta aaccgatgat agcgaggaga atgttgaata ccataaaatc
     2881 cacctccata ctatcttttt catttttatc atctatacag cttctatgat aaattattct
     2941 aaactcgtca atatgcatct tcgcatatat gtgtacaaaa aacatgcaca actttattct
     3001 aatgtttgta ccccgtagtg tcaactttaa ataaacaaag aaacggcttt ctacttattc
     3061 attaaacgat ggtttttaaa ataaatgaca ctagtttcat ataggtaaac ctttaaattg
     3121 agaaactttt ctattaaatt attctttgta gatgaacaga tatttttatt ttatgtaccc
     3181 actcgtataa ttaaataact cacgatttct ttattcaata aaaaaacggc aggtcaagga
     3241 gagtacctac tcttcagacc agccgttttt gtttatcata atttttattc ttctactaaa
     3301 ggcaattctt cttgtccttt ttcatcttct gggacagcga cttcttcacc aataccataa
     3361 gctgctctaa ctagagcaga aacttctgcc atcatttcag gatgattagc catatagatt
     3421 ttagcgtttt cccgtccttg accaatccga tcttctttat aagaatacca agcaccgctt
     3481 ttatcaacaa tgtctttttc tactgccatg tctagaagtt ctccttcttg tgagatccct
     3541 aaaccataca tgacatctac ttcagcgatt ttgaaaggag gtgctacttt gttttttacg
     3601 actttgatct ttgtacggtt accaacaata tctgtacctt gtttcaattg ttctgcacga
     3661 cgtacttcca aacggatcgt tgcgtagaat ttcaatgcgc gtcctccagg agtgatttct
     3721 ggattcccaa acatcacgcc tactttttca cggatttggt tgatgaaaat agcgattgtt
     3781 tttgttttgt tgatcgaacc agagagttta cgcaatgctt gagacatcaa acgtgcttgt
     3841 aacccgacat gtgagtcacc catttcgccg tcgatttctg ctcgtggaac taaagcagca
     3901 actgaatcga ctactacgat atctacagcc ccacttgata ctaaagcatc agcgatctct
     3961 agaccttgtt ctcctgtgtc aggctgtgaa agaagtagtt catcgatatt cacacctaat
     4021 ttttgtgcat attgcggatc taacgcatgc tcagcatcaa tgaaagcggc cgttccgcca
     4081 tttttttgta cttctgcaat agcgtgtagt gcaactgttg ttttacctga actttcaggt
     4141 ccatatactt cgatgatacg tccacggggg taaccgccga cacctaaagc gacatctaac
     4201 gcaagagagc cacttggaat tgtagagatt tgttggtcga ttttttcgcc caatttcata
     4261 atagagcctt taccatagct cttttcaatt tttttcagtg cagcatctaa ggctgcttta
     4321 cgatcatctg ccaatacgat aatcctcctt atatttacaa atcagagcaa catttcatta
     4381 tccttatcat atctttttta gtgaaaaaaa gcaagaaaaa accgaacaaa tattcgcatt
     4441 tatcattttc ttaaaagctg ttttctcaaa agatcaaatc cttgcataac cgcattatat
     4501 ctgatagcag cacgatctct ggcaaagtga tatttttccg aaaccacgcc gtctttagat
     4561 gaaatagcga tccatactgt tcctgcagat tgaccttcta acgtgtccgg tccagccact
     4621 ccggtgaaag ctacactgta atctgttctt gctatctcac gtgcttgaat ggccatctgc
     4681 tccacacatt ctttgctgac tgttccatat tctgctaata gctccggatc aatctccaga
     4741 aaaccagctt ttgtttggag cgagtaggta acgaatccgc ctggaaatac ttcagataca
     4801 ccagcaatgt cgcctaatgc ggcttggaag gcgccggctg tcaaactttc cgctgctgtt
     4861 acagtctgct tgttttcttt cagtagttcc actacgactt ttgctagcga attatcatcg
     4921 ccgtatccat aaaaatattc ccctactcgt tcttggattt tttcttctaa tgctaaaaga
     4981 gcttgattcc ctgcctgaac atcatttgtt ttcactgtca gtcgtaatgt cacttcattt
     5041 ggtttggcat atggtgcgat cgttggattg atctgtgtct caatcagatc ttttagctca
     5101 gtgaccagtc gcgattcacc aatcccataa aagcgcaaaa ctttagaaat tagtttttct
     5161 tcagaaggaa aacgttcctc tagtaaagga cgtacttgtt ccacaaacat tggtttcaac
     5221 tcattaggcg gaccgggaag aaggatatac gcatgtgtat ctgtttgata aaaagtccct
     5281 aaagcaagcc ctgtacgatt tggtagagga accccgcctt cgataatctg ggactgttgc
     5341 agattgtttt tggtcatttt tcgatgggtt gtttcaaaat atgccaataa ttttttgtat
     5401 ccttccgtat tttgtatgag agacttaccg aggtgagccg cagttgcttc ttttgtcagg
     5461 tcatcttctg tcggtcctaa tccgccacat aagagtatga gttcacttct tgtttcagct
     5521 agagcaatca actcttctaa acgctgctga ttatctccta caactgtttg ataataaact
     5581 tcgataccta gatcagctag ctgttctgac aagaaagtgg cattcgtatt cacgacttgt
     5641 cctagtaaaa gttctgttcc gacagctatt atttctgctt tcattacttc ccgctccctt
     5701 atatagtata cgctgtattt ttatattttt ttatttaaaa agagggcaag gagctgtgac
     5761 aaaaatcttg tcacagctcc ttgttccaaa taaacagtgt tctcctgcca aaacctcggt
     5821 cataagtcac aatatacata aaacatgaaa agctgttttt tgaaattatc ccttgtgact
     5881 cagagttaga cggcaggctc acaacctctc cactaaacga tacttacatt gatcctttga
     5941 acacaccgct gtttttgatg aagtaatcca cacctgaata gatagtaaag atcagacaag
     6001 cataaagcat gatttgatct actggaatac cgatcaggct gaatgggata ttattgataa
     6061 acagcaagat aatagcaacc atttgtgtag ccgtcttgac ttttcccggc caagctgcag
     6121 ccatgacttc tccatgttcg acaagtagta aacgcaagcc tgtgacggct aattcacgac
     6181 agacaatgat cgctactacc caaccagggg cctttccttg gtcaaccaac atgataaaag
     6241 cagtcataac tagcatttta tctgccaacg ggtccgcaaa cttgccaaaa ttagtgacaa
     6301 ggcctcgcgc acgggcaatt tttccatcta accagtctgt gatactagct aaagcaaaaa
     6361 taactgcacc tactagctgt gtgacagcca gtgtggtgtc tcctacgtta aattgtcccc
     6421 aatctaaagg taaacctaca accaaaatga aaatcgggat catacaaatt ctaagtacag
     6481 ttaatttatt tggtaaattc acactatcga ctcctttctt ccctattcta cgtgactatc
     6541 agcgaagttt ccaccgatat tatttacttt tccaaatgaa tttattcaaa ggagtatttt
     6601 tattgaaatt gatctttttg aattaacgaa gaaggaccgc gacacagatt tgtcacgatc
     6661 cttctttttt atacctacaa gtaaacagat gcgtaaacta atttatacga tcattctgcg
     6721 tattgaagag taaggttcac attttttgga ctctgattgt tttcccctgg attaactggt
     6781 acggattctc cattcgcttt gatcgttgca ttgcttgctg caccaatcgt gatggtcgca
     6841 tttgttgctg tttccggtaa cacggtactc tgtgtttcat ttgctgccag tgtcccttga
     6901 taaacatagg cattgtctac tagcacacct aaccatacac gattgacagc agtaaattca
     6961 agggttacag gcttggtagc gtctgtgata tttactgcga tggcactgcc agaatcttct
     7021 cctgtagcta ttgccatttt cttttcttca cttgattttg ttgttgattc tactgtagaa
     7081 gactctttgg tctgcgtgct ttgctctgag gaagaggact cctcgactga tccttctact
     7141 ttaagcgaag aagctgtgtc tccgattatt ggatcagctt gacgatcttg ccaagtcata
     7201 tagcccacga caacgacaat cgccaaagca accaatccta ataaaatgac aggaagactt
     7261 gtccaaaagc gacttggatg cgattcttcc tgatgcattg cttttctaga agtacttact
     7321 gtttcaggct gcggacgttc agggaccgat cctgtcaatt tgcttttccc atcaaaaaca
     7381 tcgactaaat ggtctccatc ttctcctaca gcttcagcgt attgccgaat aaatgcccgc
     7441 acgtaaaatt taccaggcaa ttggtcaaaa tcatcattgt cgattgcttc caaataacgt
     7501 ttttgaattt ttgtaatctg ctgtaattca tctaaagata tttttttatt caggcgtgct
     7561 tgccgtaatt tttctccgat ggtttcaaaa gccactcgtt catcttctcc agtctgtttg
     7621 attctccgaa gcatctgctg ttgcaacgaa agacgttatg tttgggtgct gtgacaaaag
     7681 tcatgtcaca gctccttttt tccgaataaa cattgttctc ctgccaactc ctcggtcaca
     7741 agtcacaata tccaataaat ataagaagct gttttttgaa cttgttcctt gtgactcgga
     7801 gttaaacggc aggctcacga cctctttctt aaacggtgtt caatcagctg ttcctcggta
     7861 ttagctcaaa aaccggcaaa atataaggag atatttttcc gtatgtttct ctaatactca
     7921 ggtcaaaacg ctgatttcac aacctcttcc ttaaacggtg ttcaaaagct tgcatctgcg
     7981 gtaataagtc caaccaagtt aaaaacatta agaagctgtt tttacttggc tgttcttatt
     8041 acttgatgca gggcagcttt ttcacaacct cttggtacac ggtgttccag aagtagcttc
     8101 ttcggcaata agccgaaata tcacaaaaat ttgagaagca attttcgtaa attccttctt
     8161 atttcttgaa gctgaccact tctgtcacaa cctctttgat tacttcgttt gattttattt
     8221 taatataagt tgattaaaag agagcatgat ttgttttatt ttctcactaa tttttccaac
     8281 acaactaaga ataccataaa agctgatttt ggaggaggtt cctactacaa gtctaaaacg
     8341 aaatttaaga cttttcctca taaaatactg gtttattttg gtctcatgta aaactcgctg
     8401 aaggcctctt cacttataaa tgcttcccca gctgccagta cgtctttcat ttggatcgaa
     8461 tcgatgattt caggaagatc aaataaagtt cgatcgccaa ataaactttg cgtaaattga
     8521 ttggcaatat attcgatcga atttaatgat tggaaatatt gtccaagcat tttcttcttc
     8581 agcaaatcga gatttgtttc tgacacttcc ggatcatcag cgaatccaag aatgatttct
     8641 ttgacttttt ctgctgcttt ctctggttcg tccgtatctc cgctgaagtc cgcaaagtga
     8701 aattcacgat ccaagctgaa ttcaaaacca aagctgtcat cgataattcc ttgattgtac
     8761 atcgctagat agtttcgaga agtattgcct aaaatcattt ggaacaacag attgattgct
     8821 gttttatagc gtaacagttc tgttccttct tgcggcaatg tgtctaatcc tttaatacca
     8881 agtacaaatt tatctcgtgt aatggctgct tccaatgcac tttgtttgat gatttctttt
     8941 gtgttttcag ggaaataacg tacaatttct tgctttggag ggaaattttt tgcttcttgg
     9001 ttttcccgaa taagtttcat caatttctca ggctccatct taccaactac aaataaaacc
     9061 atattgcttg gctgataaaa cgtccgatag cacgtataca agtcttgagc tgtgattttg
     9121 tcgatacttt caaccgtgcc cgcaatatcg atatgcaatg ggtgtgtagg atacaagtta
     9181 tttagtatgc caaaaaacat ccgccagtta gggtcgtctt catacatttg gatttcctga
     9241 ccgatgattc ctttttcctt gtttacagtt tcttctgtga agtaaggtgc ttgtacaaaa
     9301 tcgatcagag tcgtcagatt cttttctacc tgatccgtcg tagaaaacag atagctggtt
     9361 ttcgtaaaac ttgtgaaagc attggcggaa gctccttgtt tcccaaataa ttgaaagaca
     9421 tcaccatctt ctttttcaaa caacttatgt tctaaaaaat gggcaattcc atctggaact
     9481 ttcacctttt ctttttcacc atagggaata aattcgttat cgatcgaacc ataattcgta
     9541 ctgaacaaac catatgtctt gtgatagtca tttttaggca acaaatacac ggtcaaaccg
     9601 tttggcagta cttcatgata aagtgtttca ttgatttgct catattctgt tttattcatt
     9661 tgcatttcct cctgctaaaa agaagattgc ctgcaattcg atttgctcag ctacttgttg
     9721 tatctctttg atcgttacgc tctccatacg tttcaaccat tcttcatcag ataattttgt
     9781 ttccggcagc cagctgtcca aaaaagaagc ttcgattgcc gcttgcggag agtctagaga
     9841 aagcagatat tggtttctca gcattgcttt tgtctgagac agttccaact ctgttatttc
     9901 accatttcgc aaactttcta attgctcgtg aatcagatga agtacttgat ttcggttctt
     9961 ttcatcgata ccggtttgga cactcatgaa tcctcggaac gtatccacgc tactagaggc
    10021 ataataagct agactttctt tctctctgac attcatgaaa agtttagagt gaggaaaacc
    10081 gccaaataaa ccgttaaaga ccattagcgc aaaacgttct ggttcatcgt aatacacatt
    10141 tgtctgataa gctaggttca gctttgcttg aacgattggc tcacgtactt gtcgttcctt
    10201 cacgatattc acttgaggtt gggtataaaa cagatctgga taaactgttt cccgcgactc
    10261 aaatggtaat tgcccaactg cttcagctgc tttgtccgga tcaacatcgc cgacaacaaa
    10321 aatgtcgatt tgatcatttg ccatcatttc ttgatagtag gacgctagcg aactagcagt
    10381 gatcttgtcc aaatcttcga ctacgcccaa ccctgggatc ttttgttccg ggctgttttc
    10441 aaaataaagt tcttgcaagg cataggaagc aaaagtttgt ttatcttcct tcaaactttc
    10501 caaatataga cgcagattgt tcttttccaa gtcaaacgtc aactgatcaa actgatgatt
    10561 tttaatattt ggataaaata aaacctcttt taaaaaatct accgcttgag aaaaaaggtt
    10621 tggatcattg acatattcac cattcacaaa actgatccct gcgtttagcc aatgtaaatt
    10681 tccttttttt ctaactgata atccaaagct tgcgccataa agctctgcaa gttgcgcact
    10741 taactttgtc tgatccggat aattcaaact atttgtttcc aacatgctgc tgagaagtga
    10801 acgtttcgtg atgacttctt gttgcagacg cgcagtaaaa cggataaaaa tccggatcgt
    10861 tttgtattgt tttgttggta agacatgaag attcacgcct tctgccaatt gatacaccat
    10921 atttatatgc tccttttcac agttgattgc ccgcttactc ttttaatggg tcaacctgcc
    10981 ttttttactt attcctctgc ttgaaattta taagttcagg caattcgccg ttttttctaa
    11041 atgatgttac actttatagt atagcataga tgttttttat caaaagaaac agacggctaa
    11101 aagcgtatat tgttttttta ttttttattt ttcgatatac taaagacaaa taaaagaagg
    11161 gagggtcatt catgatcaaa gaattcaaag agttcatcat gcgtggagat gtcttggatc
    11221 ttgctgtcgg cgttgttatt ggtagtgcgt ttacaagcat cgtaaaccaa gtggtgcaag
    11281 gcttgatcac accactaata ggttggtttg tcgcaatgat cacaggtact tctgatttag
    11341 aaggagcact ttcaattctt gactggtcgc cgacaaaagg cgtcacattt gcgtttggta
    11401 acgttattag tgctatcatt acatttctga tcactggttt tgtcttattc ttagttgtca
    11461 aagcggcaaa ccgtgcgaag aatttacgct ccaaagaaga gcaagtggaa gtggaagaaa
    11521 ctcctactgc agaagattat cttagtgaca tacgcgatct tcttgccaaa caagtggcag
    11581 aagacaatac acatccagta gagcaaacaa atctttccga tacttctaaa taataaagaa
    11641 tctaaaaaaa agaaagccga ttttcagtgt cggctttctt tttttagatt cttaacttgc
    11701 tacttgttcc tttttcatgt cgatatgtgg aatatcatct tccaaataca catctgagat
    11761 tgccttgaat ccaaacgact cataaaaagc ttgtaggtat tcttgtgcgc cgatcacgat
    11821 tggtttttct ggaaaatttt tctggatcat gttaatcgtt tcttgaacaa tctttcttcc
    11881 taaaccatct ttacggttgt cctgatgaac aaggacacgt ccaaaatgga tactctccgc
    11941 ttcttcatat actcgcgtgt aggcaaggat cttcccatca gtatcttgaa aaaacgtatg
    12001 ccaagcttgt ccatcaatgt catcaacttc ttgataagga cactgttgtt ccacaacaaa
    12061 aactgcgatt cgttctttga cgatcgcgta aaactctgct gttgacaatt cttcaaattt
    12121 ctttacctga tattccacta agcaaacgcc ccctgctttt ttattcttag cataccgatt
    12181 cgcacacaaa aaagcaaacg aaccttacga atc
//
LOCUS       DACKAC010000080        11902 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.081, whole genome shotgun sequence.
ACCESSION   DACKAC010000080 DACKAC010000000
VERSION     DACKAC010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.081"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(177..971)
                     /locus_tag="I0B46_002356"
     CDS             complement(177..971)
                     /locus_tag="I0B46_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4428 domain-containing protein"
                     /protein_id="HAR2084613.1"
                     /translation="MSKQCAKCGGKIGLTSYKIKDKQIVCGNCMKKAGYGMTTPYKII
                     RSLNLEDLDKDPSEKRKLNISDHTAQNNHDIGNDRMRTTEEMVEFCLKYGYGKGMTKK
                     WTTHHFNLISDQLNSDEYVIFCFVGLHNYISSTKHDNNYAYALTNKRLIVAQQKMIGN
                     NVQSIILDNLNNISKKRGVLLGTLTIDTLGKVLNVAVDKDTVDRISDSLNEIIYNLKR
                     RNISATNSNTSTISSASEIRKYKELLDDGIITEEEFNKKKQELLDL"
     gene            1590..4424
                     /locus_tag="I0B46_002357"
     CDS             1590..4424
                     /locus_tag="I0B46_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAR2084614.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            4615..5106
                     /locus_tag="I0B46_002358"
     CDS             4615..5106
                     /locus_tag="I0B46_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAR2084615.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            5209..6198
                     /gene="manX"
                     /locus_tag="I0B46_002359"
     CDS             5209..6198
                     /gene="manX"
                     /locus_tag="I0B46_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAR2084616.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            6231..7034
                     /locus_tag="I0B46_002360"
     CDS             6231..7034
                     /locus_tag="I0B46_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAR2084617.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            7055..7969
                     /locus_tag="I0B46_002361"
     CDS             7055..7969
                     /locus_tag="I0B46_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAR2084618.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            8052..8426
                     /locus_tag="I0B46_002362"
     CDS             8052..8426
                     /locus_tag="I0B46_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAR2084619.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            8565..8810
                     /locus_tag="I0B46_002363"
     CDS             8565..8810
                     /locus_tag="I0B46_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAR2084620.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            8982..10046
                     /locus_tag="I0B46_002364"
     CDS             8982..10046
                     /locus_tag="I0B46_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAR2084621.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            10292..10717
                     /locus_tag="I0B46_002365"
     CDS             10292..10717
                     /locus_tag="I0B46_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAR2084622.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            10710..11162
                     /gene="nusB"
                     /locus_tag="I0B46_002366"
     CDS             10710..11162
                     /gene="nusB"
                     /locus_tag="I0B46_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAR2084623.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELGLFVK"
     gene            complement(11226..>11902)
                     /locus_tag="I0B46_002367"
     CDS             complement(11226..>11902)
                     /locus_tag="I0B46_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAR2084624.1"
                     /translation="YFSHFSREIREAVEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHI
                     VQHIGRTFRNHRIKETNQLLKSKEQKHYQLGKQLKRYWKLLQKDERKLDYTRRLWRPG
                     FKAHLTETDIVDRLLKGSPALRVGYQLYQDFLYAVKERDYVSFEELLTNNIMLPEGYQ
                     TTLRTFQKFLPQIKNALQQSYSNGPLECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHG
                     NALIFN"
BASE COUNT         3845 a         1953 c         2504 g         3600 t
ORIGIN      
        1 ccacacctta taaaaatctt tgacaaatct attcgaagga tcgaatttaa accataattg
       61 tgtttttcct tccattaaca tataaagttc acctcaaaaa gagtatacga acaaatgttt
      121 gtattgtaaa tagaaaaaga tgagctaaat taaatgccca tcttctttga tagatattat
      181 aaatctaata attcttgttt ctttttatta aattcttctt ctgtaatgat tccatcatcc
      241 aatagttctt tatattttct tatttctgaa gctgaggaaa ttgttgatgt attggaattt
      301 gtagcagaaa tatttcttct ttttaaatta taaataattt cgtttaaaga atcacttatc
      361 ctatcaacag tatctttatc aactgcaaca ttcaatactt taccaagagt atcaatagtt
      421 aatgtaccca acagcactcc tcttttttta ctaatattat ttaaattgtc taatataata
      481 ctttgtacat tattgcctat cattttttgt tgagctacaa ttaaacgttt attagttaat
      541 gcgtatgcat aattattgtc atgcttagtt gatgagatat aattatgcaa tcctacaaag
      601 cagaagatta cgtattcgtc actgttaagt tgatctgata ttaagttaaa atgatgagtt
      661 gtccattttt tagtcattcc tttaccgtag ccgtatttaa gacaaaattc taccatctct
      721 tcagtggttc tcattctatc attacctata tcatgattgt tttgtgcagt atgatcagat
      781 atgtttaatt ttcttttttc tgatggatcc ttatctaaat cttctaaatt caaagatctt
      841 attattttat aaggagtagt cataccgtaa ccagcttttt tcatacaatt gccgcaaaca
      901 atttgtttat ctttgatttt atatgaagtt aagccaattt tacctccgca ctttgcacat
      961 tgtttactca cattagttcc tcctagatat cagctttcta ctactattaa cattctatca
     1021 atagtgacca tactttgacc atacttttta gaaagaatag aaaattccaa gatatttaat
     1081 aatagaagaa aagcactaac atcatgggtt cagaagaatt gagagcttat caggaaacag
     1141 tataattgaa gtgtctgttt atccagtagc tcaagaagct taattgaatc ttatgattaa
     1201 agaggttgtg acaagacttt tgtcacagcc ttttttgttt ataaaaataa aagttgtagt
     1261 ggaggctacg aagtttactt tgtcttttta cacttaatat ttttttgcca atttggaatc
     1321 aggatgtttt gataactatc gttttcaatt gatggaagct atattgtttg ctatcagtga
     1381 aataattata tggtaaacag tgttcttttt cttgtttcca ttataaaaat cgtatcggga
     1441 tttttcgttc ttttaaattg aacatttcta tctatccgcg tgttatcaac aaaacaaaat
     1501 caggtaaata gttgacgaaa cagaaataaa tacatagtat tatgttttga agcctaagta
     1561 caatagtcta tagaaaagga gcgataccat tggcaaaacg cattgaccga atttatatgt
     1621 atgtcaaaga aaaaaccgct catttaacaa aggcagaata tgatcaagga gtaacaacac
     1681 aggaaattgc tgaagtgtta gggattcagc gaacaaatag cagtaaagat ctgaatcagc
     1741 tagtgcgcga aggaaaactg ataaagacag acggacgacc agtacgttac atatatcaaa
     1801 cactcgttac gcacgtaaag ccagttacaa aacatgtagt cagttacaaa gaaacgccgg
     1861 tctctgaaga aaaaaataca ctccaaatag atactaagga tatctttgca aaaatcattg
     1921 gagctaacgg cagcatgaaa aattcagtgg aacaggcaaa agctgcgatt ttgtatccgc
     1981 caagaggatt aaattgtttg attacaggac ctaccggatc aggaaagact tattttgcac
     2041 acgcgatgta tcattttgca aaagcgcatc atgtaataga tgaggaaacg gaactgattg
     2101 ttttcaattg tgctgattat gccaataacc ctgagctttt gatgagtcat ttatttgggt
     2161 acgcaaaagg tgcttttaca ggcgcagatg aagaaaaaat ggggattatc gatcaagcgg
     2221 atggcggtat gctgtttttg gatgagattc accggttgcc gcctgaaggt caagaaatga
     2281 tcttttactt tatggatcat ggaacttaca gtcgtcttgg ggaaacaacg aaatcccatg
     2341 aagcaaatgt acggatcgta ggtgcgacca cagaagaccc aggttcttct ctattagaaa
     2401 cttttgttcg gcgtatccca atcaatatca aacttccggc ttttgaaaaa cgtcctgcaa
     2461 atgagaaagt cgatttagta aaaatcatga ttgcccacga agcaaaccgc acgcagcgaa
     2521 agatttcttt gacagaagat gtagcaaaag cattgatcgg cagtgtgacc tatggaaata
     2581 taggacaatt gaagtcaaat atccagttag tatgtgcacg aggatttcta aatcatatgc
     2641 aatcacccga aatctcgatc acaatcgatg atttgacaga agggattcgt agcggattga
     2701 ttcaattagc aagcaaccgc agtgcaatgg cagagctttc taaaatattg gagccaaaga
     2761 taactgtagc accaaatgat acgatcatga aaatccaatc ggactcctat gagttacctt
     2821 ataatctata tgatatcatc ggagataaag cagctttact gaagtcggat ggtttggatc
     2881 aagaagcaat caatcatttc atttctacag atatcaacat ccatctgaag tctttctata
     2941 aagaccatgg tttttctttt aatgcagaca gcaaattagc agaatttgta gatccgaaaa
     3001 tcatagaagt gaccaatcag atttatgtaa tggtcaaaag atctttacca tatgagtttc
     3061 agcaaaactt tatttatgct atgagcttgc atatcagttc ttttttgaaa aaaatcaact
     3121 taggagaaga acggcatacg aatgataaca tccgtgagat ggcttttgat tttccagatg
     3181 aatatgctgt tgcgaaagaa gtgcgtagtt atatcgaaac gtattttcaa gtggagattc
     3241 cagaaagtga agactattac ttgaccgttc tactcgtctc gttacgggca aatcaagcaa
     3301 gcggcagaat cggtgtagtc gttgcagctc acggaaacag cacagcaagc agtatggtcc
     3361 aagttgtcca acagttgtta gatgcagaga acgtacgagc agtagacatg cctttggata
     3421 tggatccgaa aacagcattg gaccgaattg aacgcaatgt acaagaagta gatgaaggaa
     3481 gcggggtcat actcttagta gatatggggt cccttgcgtc gtttaattcg caaattcaaa
     3541 gagacactgg aattccagta cgtactgttg atatggtaac aacttctcta gttttggaaa
     3601 ctgtacgaaa ggtctcagta ttagggacag atctagatat gttgcatgaa tcattgaaaa
     3661 attttcgagg ttacgcagaa atatcttctg tagaatctgt tcaggaagca aaacctattc
     3721 ataagaaaaa agctgttttg gctatctgcg catctggcga aggtacagca caaagaatca
     3781 aagaattgat cgaacgagcg gtaaacaagc gtcaagaaac agaattagaa gttctagctt
     3841 tgtcgattgt ggaagtgaaa gaagaactgc cagttatcca aaaagaatat cagatcatag
     3901 ctactactgg gattactgat ttgaatatcc aagccccatt tattccttta gaacgtttta
     3961 ttgatcaaaa tatcgaagtc attttggatc agttgctgat ggaatcagaa ttggaggaaa
     4021 ctgaattcat ctccctagat gaagagtcag ctaaaaatac gtgtgttgag tttatctctg
     4081 ataacttcat ctttattaat ggtagtaagt taatcgatcc aatgtggcaa ttttctacac
     4141 aaatctctca gacgacggga atcggcgatg aagaatatgg attcaaaatc aacctagtga
     4201 tgcacacagc aggaatgatc gaacgtatta ttcggaatga accattgact gtggaagaaa
     4261 acgaattgac aaatacaacg aatgatcctc tttatagcca attggcagcc tcagttgttc
     4321 tgttggaaga tcagataaaa gtaaaagtgc cgatagaaga aatgtattat ctgctacgtc
     4381 tagtccataa tcagttagat aaaaaagaat acactgtgcc atgaatacac aaaaacgata
     4441 cacttttata gtgtatcgtt tttgtgtatt ggttaagaat cctttaaatt caattagtgt
     4501 atcgtaaaaa agttggcacg ctttttgctt atagtagtgt gtgctaggag gtggttgttc
     4561 acacagaaat gataggggtt tctgtgaagt agtgaggaag aaggagtttt gcagatggat
     4621 attcgattgg cacgtataga tgatcgttta atccacgggc aagtcgcaac agtttggtcg
     4681 aagcaaacag gtgttgaacg tatcattgtg atcagcgatg tagtcgctca agataaattg
     4741 agaaagtttc tactaaaaga agctgcaccg ccggggatca cagcaaatgt aataaccgtg
     4801 cagaaaatga ttcaaatcta tcatagtgga ttattatcac gagcaaaagt gatgctttta
     4861 ttcaccaatc cccaagatgt tgaagcggtt gtccgctgcg gtgtacagtt gaagtcgttg
     4921 aatatcggcg gtatgagttt tactgaaggc aaatcaatga ttaccaattt tgtttcagta
     4981 aatcaacaag atattgattc ctttcgttac ttgaacagtc aagaagtgga gttagagatt
     5041 cgaaaagtac ctgctgacag gaaaatcctt ttgatggaac tactagcgaa aatcaaaaag
     5101 gtgtagcaag aatggttgtt ggaaaaggtc aaataattct gttgtaatgg ttagcaatta
     5161 ttaatatttt tgaatttgtt acaacaaaag taaataggag gtataaatat ggtagggatt
     5221 atcctagcaa gtcatggaca atttgctgaa ggaatcttac aatccggttc aatgatcttt
     5281 ggcgaacaag aaaacgtcaa agccgtgatt ttgaaaccaa gtgaaggtcc agatgacttg
     5341 agagccaaat tggaagaagc ggttgcttct tttgataatc aagacgaagt gttattctta
     5401 gttgatttat ggggcggaac accgttcaat caatcaaaca cattattcga agagcacaaa
     5461 gataaatggg caatcgtcag cggtttgaat ttaccaatgt tgattgaagc ctacgcatca
     5521 cgcttttcaa tggaatctgc tcatgaaatt gcagcacaca tcattgaaac agcaaaagac
     5581 ggtgtaaaag taaaaccgga agaattagaa ccggcagaag caccgaaagc agcagttgaa
     5641 gacgcacaac ctaaaggagc acttccagaa ggaactgtgg tcggagacgg aaaaatcaaa
     5701 tatgtattag cacgtgttga ctcacgtctg ttacatggac aagttgcgac agcttggaca
     5761 aaagcagtgc aaccaaaccg tattatcgtt gtttcagatg cagtatcaaa ggatgatctg
     5821 cgtaagagac taatcgaaca agctgctcct ccaggtgtaa aagcaaatgt tattcctatt
     5881 agcaaaatga tcgaagtagc aaaagaccct cgttttggaa ataccaaagc attgctttta
     5941 tttgaaaatc ctgaagacgt attgacagct gttgaaggtg gcgtagacat caaagaatta
     6001 aatgttgggt caatggcgca ttcggtcggt aaagtcgttg taagtaaagt attgtctatg
     6061 ggacaagaag acgttgaagc gtttgagaag cttgaacaaa aaggtgtgaa attcgatgta
     6121 cgtaaagtac ctaatgattc acgcgataac atggatgata ttttgaaaaa agcaaaagct
     6181 gaattagcaa aagcgtaata aataatatta aaatttatag gaggcttatc atgtctatta
     6241 tttcaataat tttagtcgta ttagttgcct ttctagctgg tatggaaggt attcttgatg
     6301 aatttcaatt tcatcaacca ctagtggcat gtacattgat cggtttagtt accggtaacc
     6361 tcgaagctgg tatcgtactt ggcggtacgc ttcagatgat cgctttaggt tgggcaaata
     6421 tcggtgccgc tgtagcacca gatgctgcat tagcatcagt agcatcagca attattttag
     6481 ttttaggtgg acaaggtgtt aaaggtgttc cttctgcgat cgcgattgcc gttcctttgg
     6541 ctgttgcagg gttgttctta acaatgatcg tacgtacaat cgctgttcca atcgttcatt
     6601 tgatggatgc ggctgctgaa gaaggaaaca tccgtaaggt tgaaatgtgg cacatcattg
     6661 ctgtatgttt acaaggggtt cgtatcgcgg tcccagcagc agcgttgcta tttatcccag
     6721 cacaaactgt gcaatcattc cttgaatcaa tgccagcatg gttaacagat ggtatggcta
     6781 tcggtggggg aatggtcgta gctgtaggtt acgctttggt aatcaacatg atggctacaa
     6841 aagaagtatg gccattcttc gttattggtt tcgttgtagc tgcaatttct caattaacat
     6901 tgattgctct aggtgcttta ggtgttgcat tagctcttat ttacttgaac ctttctaaaa
     6961 tgggtggttc atcaaataat ggaggcggaa gcaactctgg tgacccttta ggcgacattt
     7021 tgaatgacta ttaatcttga aggaggaaaa taaaatggca gaagaaaaaa tcaaattatc
     7081 aaaaaaagat cgtttagctg ttgcgtggcg ctctaccttc attcaaggtt catggaacta
     7141 cgaacgtatg caaaacggtg gctgggcatt ttctatgatt ccagcaatca aaaaattata
     7201 caaaacaaaa gaagaacgtt ctgcagcatt aaaacgtcac ttggaattct ttaatacaca
     7261 cccatacatt gcttctccaa ttttaggggt tacattggct ctggaagaag aacgtgcgaa
     7321 tggcgcgcct gttgatgatg tagcgattca aggggttaaa gtaggtatga tgggtccttt
     7381 agctggtgtt ggtgacccag tgttctggtt tacagtacgt ccaatgctag gtgcactagg
     7441 tgcttcatta gctatgggag gaaatatcct tggaccaatc atcttcttct taggttggaa
     7501 cattattcgt tgggcattca tgtggtatac acaagaattt ggctacaaag ctggttctaa
     7561 aattactgat gacctttctg gcggattgtt acaagacgta acaaaaggtg cttcaatttt
     7621 agggatgttc gtattagctg cccttgtaca aagatgggta tcaatcaaat tcttaccagt
     7681 tgtatcaact gtaaaattag ataaaggtgc ttacatcgaa tgggataaat tgccttcagg
     7741 tggtgaagga atcaagagtg catttgaaca agttaataat ggcttagccc tttctcctga
     7801 aaaggtaaca acattacaag acaacttaga ccaattgatt cctggtttag ctgcattagg
     7861 attaacattg ttatgtatgt ggttgcttaa gaaaaaggtt tctccaatcg ttatcatcct
     7921 tggtttattc gtagtcggtg ttgttggaca cgtcatcgga cttttataat ccataaaaat
     7981 atagctcggg cgttgtcccg agcttttttt atatagtata ctgttcataa acaaatatct
     8041 ggagggagaa aatggttcaa tcaatcaata caaaagtaga cttggtaatt gatgctactt
     8101 cgtttacggg tctgacagat tacgggcaaa tcatgatagg tgataatggt tttgagtttt
     8161 accatgcgcg agatagacgc aaatatatcc aaattccatg ggaagaagtg gattatgtac
     8221 tagcatcagt catgctaaag ggaaaatgga ttcctcgata tgccattcaa acgaaaaaaa
     8281 atggtacata cacattttct tccaaagaac ctaaaaaagt gcttcgtgcg atcagagaat
     8341 atgtagatga agatagaatg gtacaatcat tgagtttctt tgatgtagtg aaacgtggta
     8401 ttcgtagtat gtttaaacgc aagtaaaaaa taaagaaaac ttctaagagg aggttttctt
     8461 tattttttta ctttgcagaa gaaaatatga aatagcaaaa ccaattattt gtccccttta
     8521 aatcccatat gctaaaattg attagcaaag aaggaggtta tattatgagt tggttatgga
     8581 cattgattgt tggagccgtg atcggagcaa ttgcaggtgc gatcaccagt aaaggaaatt
     8641 cgatgggctg tgtaacgaat atcattgctg gactagtcgg ttcaagtatt ggacaagcga
     8701 ttttcggcga atggggacca aaagctgcag gtatggcact tattccatct attttaggcg
     8761 cagtgatttt ggtagcagtt gtctcatttt tctttggaaa acgatcataa gtttttacaa
     8821 aatagtaaac taaaaggagt tgcagcgaga ctgtgactcc tttttggctt tatttattta
     8881 aaataaaaag aagttttccg gaaattttta tttaactgtc gtgtacgaag aaaagtttgc
     8941 tataatggaa aaaagcgtgc aaagaaagag ggagtttcat tatgatgcta cgtgtagaaa
     9001 aattgcgcaa aaaaatgcaa gaagaaaatt tggattcatt tttggttacc agtccgtaca
     9061 atcttcgtta cttaacgaac tttacaggta cgacaggatt agctgttatt actttggaaa
     9121 aagcattttt tattactgat tttcgttata ccgagcaggc ggcagcgcag gctcaagggt
     9181 ttgaaattat taaaaatgtc ggacctattt ttgatgaggt agcaaactta gtacgcaaag
     9241 aagaactgca ctcattagct tttgaggaaa caacagtctc atttttagaa tattcggttt
     9301 tagaagaaat catcgatgcg gaattagtac ctgtttcaca gatgatcgaa gaactcagag
     9361 aagtgaaaga tgaagaagaa atcgctatta tcgaaaaagc atgtcatatt gcggatatgg
     9421 catatgatca catcctgaaa atgatccaac cgggcatgac tgagattgaa gtcgcaaatc
     9481 aactagactt ttatatgcgt tctcttggcg cttcaggtgt ttcttttgat acgatcgttg
     9541 ctagcggatt gcgttcggct atgccgcatg gtgtagcaag caaaaaaatc atcgaacaag
     9601 gtgacttgat cacattggac tttggttgct attacgaagg gtacgtttcc gatatgacac
     9661 gtacattcgc tattggtgat cctggggaaa aattaaaaga aatttatcaa atagtcttag
     9721 atgctcaatt agcagttatt gatgctgcaa aaccaggaat gactggtgtc caattagatg
     9781 cagttgctag agattatatt tcaaaacatg gttacgggga agcatttgga cattctacag
     9841 gacatgggat cggtttagag atccacgaag gaccaaacgt ttcttctcgt gcagaaaagc
     9901 aatttgtacc aggaaatgtg atcactgatg aaccagggat ctacttacca ggaatcggtg
     9961 gagtacgtat tgaggatgac ttgttgatca cagaagatgg aaatcgtgtg ttgactcgtt
    10021 caccaaaaga attgatcatc ttatagtttc atcaatagaa gaagtaagcg taaaaatcag
    10081 tgttcacaga ctttgttata gaaaatcact cgatttaatg atacactata gaagaacttt
    10141 taagctgtaa gagataataa agttgggtgt tttctccaat tcatagcagt ttgcagctga
    10201 tcggcggcag ttttcttacc acgtgatcac tatgccgcat catagagaat tcggatgtat
    10261 ctcgtgctta ggcaaaggga ggaataaata aatggcagat gaaaaaaatt tgattttaaa
    10321 cgcaaaccaa gatttaggag agatcgtgat cgctcctgaa gtcatcgaag taattatcgg
    10381 gattgcagct tctaaagtag aaggcgtata cggaatgcgc ggaacttttg ccagcaatgt
    10441 cactgaatta ttaggacgtg cagctcacgg aaaaggtgtt tacttagcaa acgaggaaga
    10501 tggcttgaaa gtagatcttt actgttatct agaatatggt gtttcagttc ctaaagtagc
    10561 aatggaaatg caggaacggg tcagccagca agtccttttc atgacggata tcgatttatc
    10621 tgaagtcaat atccatgtag tagctgttgt tcctgaaaaa ttagaacaac caacaatcga
    10681 agaacttttt gaagacgaag aggaagagaa tgaataaaga attatcaaga cacgagattc
    10741 gggaaatggc attgcaggct ttatttccat tagacttcaa tgcagaccta acaaaagaag
    10801 atgcgatttt caacgcaatc gaattagacc accgcgacat gatcaacgaa gatgaatcag
    10861 agtttgttcc tgtttatctt gatacgctag ttggcggtgt ctgtgcaaaa aaagatgaat
    10921 tggataaagt gattgaaaaa catttaaaaa acaattggca catcaatcgg atctcaaaaa
    10981 tggatttggt gatcttgcgt atcgcgattt ttgaaatgac ttatgtgaca gatgtaccag
    11041 cggctgttgc tttaaatgaa gcaatcgagt tggcaaaaac atttagtgat gaccgttcac
    11101 gtaaattcgt caacggtgtt ttatccaatg tattgaaaga attagggctc tttgtcaaat
    11161 gatgttggta aagagaaatc ttagaataat tggagatcaa gtggatcagt tcctcttgat
    11221 cttttttagt tgaaaatcaa tgcgttgcca tgacgaatca tgatgcgcaa tttgaaatta
    11281 taaaagctac gaaagccata tgcatttcgt tttaacactt tgatatggtt gttcagacat
    11341 tcaagtgggc catttgaata actttgttgt aaagcatttt tgatttgtgg cagaaatttc
    11401 tggaacgttc tcaaggtcgt ctgatagcct tcgggaagca tgatattatt ggtaagtagt
    11461 tcttcaaacg atacgtagtc tcgttccttt acagcgtaaa gaaagtcttg atataactga
    11521 tagcctacac gcaaagcagg gctccctttc agtaagcgat ccacaatatc ggtctctgtt
    11581 aaatgagctt tgaagccagg tcgccaaagg cgtcgagtat aatcaagctt gcgttcatct
    11641 ttttgaagaa gtttccagta tctttttaat tgcttaccta attgatagtg tttttgttct
    11701 ttgcttttta atagttggtt cgtttctttg attcgatgat tgcgaaaggt acgaccaata
    11761 tgttggacga tgtgaaaccg gtcaataatc agctgtgctt ttggaaacag ctttttaacg
    11821 agcgatacat aaggagcgta catatcaatc acgatccatt cgaccgcttc acgaatttct
    11881 cgtgaaaaat gactaaaata ac
//
LOCUS       DACKAC010000081        11590 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.082, whole genome shotgun sequence.
ACCESSION   DACKAC010000081 DACKAC010000000
VERSION     DACKAC010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.082"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(117..1247)
                     /locus_tag="I0B46_002368"
     CDS             complement(117..1247)
                     /locus_tag="I0B46_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAR2084625.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            complement(1788..1994)
                     /locus_tag="I0B46_002369"
     CDS             complement(1788..1994)
                     /locus_tag="I0B46_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084626.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(2010..2624)
                     /locus_tag="I0B46_002370"
     CDS             complement(2010..2624)
                     /locus_tag="I0B46_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAR2084627.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(2771..3454)
                     /locus_tag="I0B46_002371"
     CDS             complement(2771..3454)
                     /locus_tag="I0B46_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAR2084628.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(3563..4021)
                     /locus_tag="I0B46_002372"
     CDS             complement(3563..4021)
                     /locus_tag="I0B46_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAR2084629.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(4105..5868)
                     /locus_tag="I0B46_002373"
     CDS             complement(4105..5868)
                     /locus_tag="I0B46_002373"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAR2084630.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(6089..7549)
                     /locus_tag="I0B46_002374"
     CDS             complement(6089..7549)
                     /locus_tag="I0B46_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084631.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(7757..8683)
                     /gene="glsA"
                     /locus_tag="I0B46_002375"
     CDS             complement(7757..8683)
                     /gene="glsA"
                     /locus_tag="I0B46_002375"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAR2084632.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(8706..10133)
                     /locus_tag="I0B46_002376"
     CDS             complement(8706..10133)
                     /locus_tag="I0B46_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAR2084633.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(10164..11378)
                     /locus_tag="I0B46_002377"
     CDS             complement(10164..11378)
                     /locus_tag="I0B46_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAR2084634.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         3507 a         2403 c         1911 g         3769 t
ORIGIN      
        1 atatgatttt ttgagatact ttcattgcga attcctcttt ctaaaagaac ctctcgcttg
       61 aaaaataaaa gattgtactt tatgataatt caaactgaga gtttctcagt ttctctttat
      121 tgtttttcag gtcgatagag cgccaactct tcggcctgta tctttgaaga aatattcaac
      181 gtgattccga ttcctaaaga aaggatcaga taacttgttc ctccgtagct aacaaatggc
      241 aatggcacac cagtcattgg catcagcccc gcgatacttc ccacgttcat tatcgtctga
      301 acgaatagca aagttccgaa tcctaaaaga aacaatccag cttgctgatt tttacagcga
      361 ctgcttaaac aaaagattct catgcataat gagaacaata agaaaagcac acacaatgca
      421 ccgattagcc caagctcttc tgtaataatc gaaaaaataa aatctgtttc tgtctctgga
      481 agataccctt tttttgtgat actatttcct aatccccgcc caaatatgcc accattatat
      541 agtgcataaa acgaatgggt catttgatag cccgcaccat gactatcgat aaaagaatct
      601 cttaaagtcg cgatcctttc ataagcatga ttaaacatct gtggaagcca acctttattt
      661 cctaaatata ataccccgcc tgctgctcca atcagcaatg cagaaaaagt aacgattaga
      721 tagatccctt ttttaaatgg aaccgccgct gcccaaaata tgacccacgc aatcgaaagg
      781 atcatcaatg ctccagcaat ctttggctga aataaaatca gaaaagttat acctactgta
      841 atcaggaatg gtttttttag atcttttggg ctactatttc cgtcacgaaa aaaccagctt
      901 aaataaaaaa tcaaaaaaag atttgccagt tcagaaggct ggaattgaat gccaaacaga
      961 gagatccaac gttgtgcgcc gttgacagtg acaccgaata tccctattct tactaatact
     1021 aaaaagaaaa tcgataaggc taaaccgtat cctgctattt tagggtgaag taaatagtgt
     1081 agtttgactg aacgagctaa gaagatcacg ctccaactca aaaatatgaa aattagttga
     1141 cgcaataata gactttttgt attttcatcg gcttgcaata aacgataaga actggcactg
     1201 tatacttcta aaagaccgat catggacaaa gtcaaataag gacccaaaat ccaccaatcg
     1261 atcttttttc accgcatctc ctcctcacac agcaatccac ttgtcaggtg aggagcgagt
     1321 ttgtctcatc aaataagtca gtcacaaaaa tataaagaaa aggtcagaaa ccttttcgaa
     1381 caagaattct gacctagcat catctcatcg tccgagtttt agcactatac aacgtaaaga
     1441 aaacattctt agctattgga aaaagcctta atccaacagt ttctgtttta cccattggcg
     1501 actttcgacg tttctgggca acagcctatg tttgtatagg agcctcacct aacaaggtta
     1561 ttactatatt ctatttacga gttcatgata gacttttcct aatgattagg caatggaatt
     1621 tgaaatgaaa tagtgaaatt taacaatctt taatattttt tagaaaaaaa taacaaataa
     1681 gttgaaagaa tcccgttgca aattctttca gttgcaccag gattctttga taatttttta
     1741 gttttatgtg tttttttata tgttttttca cttttcggaa taaatattta tttgttaaag
     1801 taattaaaca atccgctaat cgtttgatct ggcatttcta tttccttacc atctaaagta
     1861 acagtagtaa cgacaaaatg gtcttgcaat ggcggaactt ctaagtactg cccttgcaga
     1921 aaatcattca ctgtttcata ttcttttttc atttcttttg aatctgtcga tggtttatag
     1981 cttattttta acataactag tgctcctttt tattcgggac gtgcaatcgt gaaaatttct
     2041 cctattgctg cataatcatt tcctgccaaa cggcttacag cattcaattt tcttggatcg
     2101 atccttcctg tcttatcatc atagactgtt tcatttaaat gatatttctg tacttctagc
     2161 aaaaataaat cagctgttac tttattttta ttccgtatct cgacatgttc atataaggat
     2221 gtttcaaaac gaacttttgc ttctgtcaat gctggtactg aaatgattac tgaatcagtt
     2281 aaagtcaagt cagtcaggta cagttcgctt tcctcattag gcagattggc tgctgtttta
     2341 tttacttctt ctacgttgtc ctcatcaagg atatggataa cagcttctct tgtctcaaga
     2401 atattctttg ctgtgtcttt tactgcatct tgttttctct ggatcgccaa ggataagatg
     2461 ggtcgattcg aactgacgat attgaagtaa ctgaagggtg cgatattcac tatatctttt
     2521 gctgatttag tcgttactac tgctactggt ctagggatga ctgaaccaat cagcaacttg
     2581 taattttcac gttctgtcaa ttctgaagga ctccgttcaa acatggtttc gccttctttc
     2641 tataaaattc gttaaaaagc agatcttgaa gacaaaatga aaagtaatgc ttttttcatc
     2701 ttcttagaat ctgctttaaa ctatctatca atttttcttt tttgtcaaga atctgtgccg
     2761 tgcgtgtttt ttagttattt gctttacttg cggcttcgac atatctttta gtgatcaatt
     2821 gcggacgggt gataatagat ccaactacga ctgaaaaagc acctaattca tatgctttct
     2881 ctacttgttc aggagagtca gtatgtcctt ccataattac tgggatatcg acttgttcta
     2941 tgatttgatt caataaatca aaatcaggtg tctgcgtctt tcgtgaatat tctgtatagc
     3001 ctgctaaagt cgtagacaaa agatcaaaac catcttcttg cgcttgaagc gcttcctcaa
     3061 aagtcgaaat atctgccata gctaaccgac cgtgttcgtg aatcatttgg acaagttcag
     3121 cagttgtttt ttctgtcgga cgtttacgag aagttgcatc cagtgcaatg atttctgctt
     3181 gcgtctccaa aagttcttcc acttcttttt ctgtcgcagt aataaacact tctgacccat
     3241 cgtaatctct tttgataatc cctattaccg gtaaatcaac gattcgcttg atttcattga
     3301 tatcttctgt cgtatttgct cgaataccaa ctgctccgcc ttcttttgca gccaaagcca
     3361 ttctccccat aataaaagaa ctgtgtagcg gctcgttttc caaagcttga caagaaacga
     3421 cgactccgcc tttgatttgt tccagtgtat ccacaatatc tcctccagta atgttatata
     3481 aaaatacagc ttcatataaa aatgtaaccc ttttctattc aggagacaat aatgagctat
     3541 ttctttcagc agacctttct tactaaaaga aaattacttc aaattcttcg tatacgacca
     3601 gcttctcttc tgtaaattct atgttcaacg cttctttata agcatgttta aaaaactcag
     3661 catggacttt ccgccaataa gctaatgtcc gatccccttc tccttctttc caagcatgct
     3721 ctttcgtgac ttcactaaaa ggcaagatgg tcacatttat atttttgatg atacaaactg
     3781 gttcattttt tccattcagg accacgtcta attgaccaac ttttggtaaa ggctcattct
     3841 cttgtttata cagatcgtaa ccacttgatg taccagtctt tatctttttt acaactagtt
     3901 cgctcaactc gtctgccatt ttttctgtat ccccaaatga aaagaaacct aatttttctg
     3961 attgataact aggatatttt tcttggaact ttttgacata ttcttctact tgctgataca
     4021 taacatccac ccctttgctt tttataatga aaaaaccatc cagatactag tataaacaat
     4081 tatctggatg gtgagcataa tgaattattg aatattcaag acacgcagac tatcttcaac
     4141 gatttgtttt ggtgttaaat gataacgttc ataaagctgc cctacaggaa cgctatccgt
     4201 gaattctttc tctgcaccaa aatttaatac gttcattgaa gaatcaccat aataacgact
     4261 gatattttcg ccaaatcctc ctgacaaact accatcttct aatgtgatga ctaattcgtg
     4321 tgtttctttt aatttatcta atgtttctac gtcattttct gtgatcgttc ctggattgat
     4381 caacgttgct tcgattcctt tttttctaaa agcttttact gtttcttttc ctaattcaag
     4441 aaaaccgcct aaagctaaaa ttgctacttt tcctccatta tttactgttt gataggctgg
     4501 aatactataa tcatttgcga tagctgtacc atgttctaca ccatgtgtag gaattcggat
     4561 tgctacaggc tgcgctgttt gttcgaacgc ccaatctagc atagagatca tatcctcttt
     4621 tgttgctgga gcaagatatt tgatgttcgg gatacttgat aagtaaggaa tgtcaaatgt
     4681 tccttggtgt gttcggtcac tagctgagat cgttccgcca ccgactaaaa taatcactgg
     4741 attctcgttg atcgctaaat catgtgataa ttgatcatat gcccgttgca agaaagtact
     4801 tgattggaag acgactggac gtactccctg agaagccaaa gcagaagcga aagtgataga
     4861 gtgctgttca gcgatcccca cgtcaaaata atgatctgga tgtttttctt ggaatgtttt
     4921 cagtccaaag gcgcctggta tcgctgcatt gactgcggca ataggagctg tacttgtttg
     4981 cattttctta tccaaataat ccatgatgat catattaaat gtttctccag aagttacttg
     5041 ctttgcctcc ccggtggaca gatcaaatgg tacatgccaa tgatatttca ttttattatc
     5101 gattgccggc tgataaccgc gacctttttc cgtatggacg tgaagtacaa tcggatgatc
     5161 aatatctttt acttcttcaa ataaacggat caccgtttcc agatcgtttc cattatccac
     5221 gtaacgataa tcaaatccca ttgctttaaa taggttattg ctgcttttac cgttggtttg
     5281 acgaagctca gctaagtttt tatataaact gccatgattc tcatcaatag acatctcatt
     5341 atcgtttact aatacgatta gattggattg taaaacacca ccattgttca atccttcaaa
     5401 agctaagcca ccacttaggg agccgtcacc gataaatgct accacatttc ctgattcttt
     5461 cttcagatct ctcgctttag ctaaacctgt tgctaagctg atagatgtag atgtgtgccc
     5521 cactgtaaaa tagtcatgtt tactttcatg ttggttggaa tatccgctca cttcacggta
     5581 atgtcctttt tcaaaaccat tttttcgacc agttaaaagc ttatgaccat aagactgatg
     5641 ggaaacatcc caaacgattt tatcctttgg tgaatcaaat acataatgaa acgcaatcgt
     5701 taattctact acacctagat tcggaccaac gtgcccacct acatcgctgt ccttttgcaa
     5761 gattttttgg cgcatctctt ctgccaactg tttcatttcc tcaacagata attttttgac
     5821 atctgcgggg ctattgattg catccaatac atgtaatact gtactcataa tacattctct
     5881 cctccgtact atcttctttt aatgaaaata gatttgttac actttatcta ttctactact
     5941 tagagttgac tctaagtcaa tagcatttta aaaaaatttc agagaaagct tgttatataa
     6001 gtagatcttc gcacgaatca ggactcaaat aaaaaccttc tacagacaat taaaaagctt
     6061 tgtagaaggt ctcctaatta aggtctcatc aagaaatatg tttttttaat aggtgaagtt
     6121 cctgagcaat atgatacaaa tggatgtttg caccttttaa tgagattgga tagatctttg
     6181 tcttttcagt ttctaatcca ttttgatagc tagcagcatc gcacaaaacc actctttttt
     6241 tcgcacatag acattcatca ggcaattctt ctaatgaacg aaataaaata ggatttattt
     6301 cagcgatgcc ttcttccgca tacaaatgaa attgggttcg ataaaaaaat tgtttgactg
     6361 aactttctga aacaacaaaa atttctattt cctgtctccc cgcttgttca ctgttgatca
     6421 aagaaagact tttgacgaat tttcgaatga aattttcatt ccatcttagt tggatttgat
     6481 ttttcttttt ccactcttcc aatatttcta caatttttcc gtacagaagt tgttcttttt
     6541 ctgatagaag atagatcctt tccggtaaaa atacttgtcc atctgcccac aacgtacgga
     6601 caaattgtaa aaaggattgc ttgaaaataa aattttctgc atcgtcgatt tcagcgatac
     6661 taataatctg taaaatcaat tcattcagtg ataggtcatt gagaacaaaa tttttataaa
     6721 caacttcgaa atcttttctt aacagctcca tgttctcatt ggtataattt ctactgttga
     6781 acaaagaaaa aatataatag acatcttctt cttgtaagtc tagaattcct ttttgctctt
     6841 ccaatccttg acagactaat tgaaataacg gcttcgtttc tgcgcttttc ttatcttctt
     6901 ctttcagtcc aaggggaaat gcctttcttc cgattgcgat ctctattcct cgtatgataa
     6961 aggacttttc atcttccgaa tatcttcttt taaaaaaatg agtctctaca taatcaatca
     7021 actcgttaca agcttcttct acatacggat ttttattttc atagccttcc caattgacat
     7081 aacgtactaa agctagcaag atgttccgcg agtcaaattc tgggataatg atttctttat
     7141 ttttcaaata atcattttct ttaaaaaaat ccaatacgat ttttttgatt gtatatactt
     7201 tgctcaaaga aataaattca agatcagcca gttctgtact cgtgaatata ccattaaaat
     7261 aataacttaa aaactttaaa aaataagatt gaccgtacag aaactgtgtg agctcagaaa
     7321 gagcaatacc atgtttgaat acgagtttat acttatgctt ctctcttact tgttctttta
     7381 tacaatattc tagacactcg gctagacttt ctagatcgtg aaggatcgtt ggtgtagtca
     7441 cccttagaga aatagctgtt tgttcgatgt ctatttcttg gaattcaaat aattgttcac
     7501 acagatacac ttttctaaat atatttcgct cgatgtaatt ttccagcatt cctgatacac
     7561 gcctccagtt tttcttatga ttttatttaa tcatatctgt aaatcgtatt tttgtttcct
     7621 ttttatttag aaaagccctt tcaaaaacca ataaaaaaag aaactgggac aaaagtcaaa
     7681 caagctcaca atttcagcct cattgtaggt agctttgagc cgtttggact tttaccccag
     7741 cctctttcaa gaatggttat tcaaagatgt cagcgtctag ttctttgact acatctttca
     7801 acaattggat accagctgcg ctgttaccaa atggatctag tgccgggctg aagacaccta
     7861 tgccgtaacg atttggcaca gctgccatca acccgccgcc tacaccgctt tttgcaggta
     7921 caccaacatg gacggaaaac tctcctgatt catcatacaa accagcagtt gtcataattg
     7981 atttgacgat tgtagcactt tcttcagtaa tgatttgttt gccattccaa ggagcaattc
     8041 ctttatttgc cagcacagca gctaattttg ctatgcctgt agcggtgaca ttgagggagc
     8101 attgtctgaa ataagcatcg agtatctccg gtacgtctcc ttcgatcatt tgattacctt
     8161 tcatatagta agcaagagag cgattgatat cacctgttct tgattcagaa tgatagattt
     8221 cttcattcaa tgtgatatta ggatcatcac agatctcttt cataaatgac aagattcgct
     8281 caaatttttc atctgcatct ttaccggcaa tcaacgaggt tgttgcaatc gctcctgcat
     8341 taacaaaagg attcattgga tgtttgcgat gattgatttc catattcatg atagaattga
     8401 aggcaaaacc ggttggttcc gtgttgattg tttgaaagac tttttctatt ccgttgtctt
     8461 tgattgctaa taacaatacg ggaacttttg acatgctttc gatggcaaag cgcacatcgg
     8521 cgtctcctcc ctcaatcagt tgttttgtat ccaaatcata aatcgccata cctaattgtt
     8581 tgggatccac ttcagctaat gctggaatgt aatccgccac ttgtcctgtt tgataatagt
     8641 ggaaattttt tccaatcaat tgttctaatt cttttacttc catttgaata accagctttc
     8701 attttttatt tttctgcatg ctggaattta tgacggcatg cgtagatcgt aaatggaata
     8761 actaagacga taagataaga aacaatcaga atcgtttcat aaatcccatg ctgtccagct
     8821 gcgatactag caggtggaac aaatgagata caaaaagcaa aaagtgaagt caaaagacct
     8881 aaacaagcaa cgattacttt tacaactttt ccgcctggaa cctgataggc tctttcaaga
     8941 ctgtcatctt taaaaaccag cttcaaatag cccgcgaaaa acataaagta caacatcagg
     9001 tagattacaa cagtcaatga catcgctgca agaaatgaaa gattattccc cccgccgctt
     9061 aatgtaaaga cggctgccca gattgttgca acgatcccat ttgcgatcat caaatgagca
     9121 ggaacaccgt gtgtgttttc ttttgcaaaa tattcaggaa gtaatccttc ttctgctgct
     9181 acgtgaagtg cttttgaagg agaggttacc caagaagcaa tttctccgat gacacctaaa
     9241 caaacaagaa tcgcaagaat tttcgcaatc catgcaagat ttggatcaat gtgtcttaat
     9301 aggaattcta acgattgaat cacccctgta ttaagagaca aaccatgttg aggtactgta
     9361 gtagcgacgg tcaatccgcc aaaagtatct aaaatgattg ccagaataac caatacaaac
     9421 attgctaatg gataatcctt tttagggttt tccatttcat ttgcatgact ggcagatgtt
     9481 tctacgccca tatatgctaa aataaaggaa acaaaaacaa ccagcgtagg caatttcgaa
     9541 aaatcaggaa tcaatgtttt catagacagt atcgtttgta gtggagcgcc tgaagcgaca
     9601 taatggatcc ctaagccaaa caagactaat gctggcaaca agataccagc tacaaaaccg
     9661 atccgagcaa tcaatgaagt gtatttggtt ccttttaact gtgagagtaa aatagcccag
     9721 aaaataatca ataggattgc caatttcaga tatttattgt tttgtatagc accaataccc
     9781 gttgcatagg ataatgcacc agttaagaaa tacagcatgg tcaagtagcc aactgttatc
     9841 tcaaaccatt gaaaaaagat tgcggcaaaa ccgaatcgtt ttcccaatgt gcgactgacc
     9901 caagtataga caccgccttt ttcccatcct ttcaccgttg ccatctctgc tgcgcacaat
     9961 gccacaggga taaaccatag caaaccgcct aataacaaga aaaatactaa gctgaagcct
    10021 gaggtcgcga atgtgggata ttcatataat gaaacgacca ttgagatggt catagccaaa
    10081 aagccaaaga acgttaattt ttttgcagga attttcgttt tgctttgttg catgaatctt
    10141 cctcttttct atttgaattt gtttcaattg agtttgttgc caaagaagaa aaagtccaat
    10201 ccttcttctt gttcttcttt tgtagtagtg atcgttgtaa agtgcatcgc cgattttaaa
    10261 atcactgttg tcagcacata ggcatacacg gcagtaatga tgacaccaat cacttgaatc
    10321 cctaagaagt accagctgct tggattcgtt agtaaatggc tgttagatgc aaagatccct
    10381 atcagaatcg tacctgtgaa tccgcccatg ccatgaacac cccacacatc aagtgcatca
    10441 tcccattgtt tctttttacg aaaatcaaca gcagcatgac agacgatacc cgcaatgatc
    10501 ccaatcagca tggcactttt tgcttctaca taaccggcac aaggcgtaat ggctgccaat
    10561 ccagcaacgg atcctgttag tacgtcgaca aaatcaaagc catttccttt tacttttgca
    10621 atgatcagcc aagtaatcat cgcaaatgca agagcgatga atgttgaacc aaatgcattg
    10681 actgcttgtt catctgctcg aagtgcacca ccggaattaa aaccgaacca gccaaaccaa
    10741 agcagtcctg taccgatagc agctgccata agactgctat gctgacttct ttctcctaaa
    10801 ggaatctttc tttgaccaag tgtgaggata catgctaaag agccgaatcc agctgtcgtg
    10861 tggatgactg ctccaccagc gaaatctcta aatcccatct gatccaaaaa tccgccaccc
    10921 catatccagt ggcaaacagg gaaataaatc agcagattcc aaaaaataac aagtataata
    10981 tagcctttta aattcaatct ccctgcaaaa gctcctgtca tcaaaggaac ggtaatcacg
    11041 ataaacatca gttggtaaag aaagaacatt aaaaatggaa tcgttgcacc atgaaacatg
    11101 ttggggaagc ttgtaacatg tctcatcaaa aagaaatctg ccgggtttcc aataacaccg
    11161 ccgatatccc gtccaaaggc caatccgaat ccaccaaata cccacataat cgttgttatc
    11221 ccaacggaga gaaaagattg agacatgatc gttagtatat tattcttccg tgctaatccg
    11281 ccgtaaaaaa aagctagacc aggagtcatc aggcaaacca ttgctgtaca taaaatcatg
    11341 aatgctgtat ctcctgtatt gataggtaat gtattcattt ttttcgcctc ctttctacga
    11401 ttacgagtat aaaagaaggt gtagttgttt ttttcttctg tttttttgaa ttaatacttg
    11461 catttataaa agacagacaa ttaataaaag cttgatgtgt tgtgctcttt ttcgtttttc
    11521 acacaaataa agacaaaaaa taaacggtct tctttttata gaaaaccgtt tattttcgtc
    11581 tttttttatt
//
LOCUS       DACKAC010000082        11342 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.083, whole genome shotgun sequence.
ACCESSION   DACKAC010000082 DACKAC010000000
VERSION     DACKAC010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11342
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.083"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..936
                     /locus_tag="I0B46_002378"
     CDS             <1..936
                     /locus_tag="I0B46_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAR2084635.1"
                     /translation="GRWKETLPEISHEINIVNLTSDEKNKGLLDPYVIMKNTKDSESL
                     AIDILTFLTGISSRDGERFPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADH
                     IESFTDYDFAHLLFSDGYVEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVA
                     MLIVISTFALDFIHTDRSIFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFV
                     TQNTDDLLDEKLKNNLGLKFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQC
                     LISDLYGRVGVIQFHPVFEELLHAFDTRPPVRKEV"
     gene            939..3122
                     /locus_tag="I0B46_002379"
     CDS             939..3122
                     /locus_tag="I0B46_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAR2084636.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            3119..4120
                     /locus_tag="I0B46_002380"
     CDS             3119..4120
                     /locus_tag="I0B46_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAR2084637.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            4135..5049
                     /locus_tag="I0B46_002381"
     CDS             4135..5049
                     /locus_tag="I0B46_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAR2084638.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            5294..5410
                     /locus_tag="I0B46_002382"
     CDS             5294..5410
                     /locus_tag="I0B46_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAR2084639.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            5426..7342
                     /gene="tet(M)"
                     /locus_tag="I0B46_002383"
     CDS             5426..7342
                     /gene="tet(M)"
                     /locus_tag="I0B46_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAR2084640.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYSP
                     VSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIVD
                     TQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRPN
                     SRIDKVRYMFNKIT"
     gene            7440..8813
                     /gene="tet(L)"
                     /locus_tag="I0B46_002384"
     CDS             7440..8813
                     /gene="tet(L)"
                     /locus_tag="I0B46_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAR2084641.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            9377..10639
                     /locus_tag="I0B46_002385"
     CDS             9377..10639
                     /locus_tag="I0B46_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plasmid recombination enzyme type 2"
                     /protein_id="HAR2084642.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            10884..>11342
                     /locus_tag="I0B46_002386"
     CDS             10884..>11342
                     /locus_tag="I0B46_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAR2084643.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINK"
BASE COUNT         3738 a         1896 c         2376 g         3332 t
ORIGIN      
        1 ggcagatgga aagaaacctt gccagagatt tcccatgaaa tcaatatcgt caatctgact
       61 tctgatgaga aaaacaaagg cttacttgac ccttatgtaa ttatgaaaaa taccaaagat
      121 tctgaatcac tggctattga tattttgaca ttccttacgg ggatttcctc tcgtgatggg
      181 gaacgcttcc caatccttag aaaagccatt cgtgcagtaa ccaatagtga agtgcgaggg
      241 ttgatgaaag tgattgaaga attacgggtt gagaatacgc cactaagtac cagcatagcc
      301 gaccatatcg aaagttttac agactatgac tttgcccatc tgctttttag tgatggttat
      361 gtagagcagt ctatcagcct tgaaaaacaa ctgaacatta tacaggttgc cgacttggta
      421 cttcctgaca aggaaacttc ctttgaggaa tataccacaa tggagttact atcggtagca
      481 atgctgattg tcattagtac ctttgcgttg gactttatcc atacagaccg aagtattttc
      541 aagattgtag acttagacga agcatggagc ttcttacagg tggcacaagg gaaaacacta
      601 tctatgaagc tggttcgtgc tggtcgtgct atgaacgctg gggtatattt cgtgacccaa
      661 aatacagatg acctcttaga tgaaaaactg aaaaataacc tcggcttaaa atttgccttt
      721 cgttccactg accttaacga gattaaaaaa acccttgcct tttttggtgt agacccagag
      781 gacgaaaaca atcagaagcg attgcgtgat ttggaaaacg ggcaatgcct tatcagtgat
      841 ttatatggtc gtgtcggtgt gatacagttc caccctgtat ttgaagaact gctccatgcc
      901 tttgatacca gaccacctgt gcgaaaagag gtgtaaatgt gaaaccatca atattaaaca
      961 gaataaaatc aaactggacg ctgaaacgtc taggtaaagt ggcaatgaca gtggctttca
     1021 cacttgtgat tgccattttt cttttagcca tgctgggaac ggtggttcaa gctgcgggct
     1081 tggtagatga tacggtcaat gtggcaaatg aatacagccg atacccactt gaaaactatc
     1141 aactggattt ttatgtggat aatagctggg gctggctacc gtggaactgg tcggacggaa
     1201 ttggaaaaca agtcatgtat ggactatatg ccattaccaa ttttatttgg acaatcagtc
     1261 tttatgtttc caatgcgacg ggttacttag tacaggaagc ctattcctta gacttcattt
     1321 ccgctacagc agattccatt ggcaagaata tgcagaccct tgcaggggtt acgcctagtg
     1381 gactatcaac agagggtttc tatgttggat tccttttact cttgattttg gttcttgggg
     1441 tttatgttgc ctatacagga ctgataaaga gagaaaccac aaaggcaatt catgccctca
     1501 tgaattttgt gctggttttt atcctatcgg cttcctttat tgcctacgct cccgactaca
     1561 ttaaaaaaat caatgacttt tcatcagaca tcagcaacgc tagtttgtca ctgggtacaa
     1621 aaattgtcat gccccattca gatagtcaag gcaaggacag tgtggacttg atacgtgaca
     1681 gcctattttc catacaggtt cagcaaccgt ggctactgct tcaatataac agttcagaca
     1741 ttgaaagtat tggtattgac cgtgtggaaa gcctgctctc aaccagtcca gattccaaca
     1801 atggggaaga cagagaaaaa attgttgcgg aagaaattga agatagaagc aataccaata
     1861 tgacgattac aaagacgatt aaccgtttag gtacagtctt cttcctattt gtcttcaata
     1921 ttgggatttc catatttgta ttcctattaa caggaatcat gattttctcg caggtacttt
     1981 ttatcatcta tgctatgttt ctgcctgtga gctttatttt aagcatgatt ccatcatttg
     2041 atggtatgtc aaaacgagcc ataacaaagc tctttaatac cattttgaca cgagctggaa
     2101 tcacattgat tattacgaca gcatttagta tttcaaccat gctctatacc ttatcggctg
     2161 gttatccgtt ctttttgatt gcttttctac agattgtgac ctttgcagga atctacttta
     2221 agctgggcga tttaatgagt atgttttctc tacagagtaa cgattctcaa agtgtgggaa
     2281 gtcgtatgat gagaaaacct cgtatgctta tgcacgctca catgcaccgt ctacagcgga
     2341 aacttggacg ctccgtgact gctttagggg ctgggtctgc cattgccagt gctacgggta
     2401 aaaaaggaca gtcgggttcg ggaagttctg cgagtacaca agcagatcac tctcgaccaa
     2461 acgggcagga aaaatcaaca ctaggaaagc gtatcggtca aaccatcggt acagtagctg
     2521 ataccaaaga cagaatggta gatactgctg gtaatttgaa agaacaggtt aaggatttgc
     2581 cgaccaatgc aagatatgca gtatatcaag gaaaatctaa agtgacggat aatgtccatg
     2641 atttaaccag aagtatttct caaaccaaag cggataaagc cagtggacgc aagaaacagc
     2701 aggaacaaag acggaaaacc attgcggagc gtcgctctga aatggaacag gtcaaacaga
     2761 aaaaacagcc agcttcttcc gttcatgaaa gaccagccac taagcaagaa caatctcatg
     2821 atggacagac ttcaaggcaa acaactgtac aggcttccta tagggaatct caacaagcca
     2881 aacaagagcg tccaacagtt aagtccgatt cttcaagttt aaaaacggaa cgtcaaagta
     2941 atacagttca agaaagaacg gttcaaaagc cagtaacttc aactgcacca acagatagag
     3001 cttcacaacg ttcaatcaca aaagaacgtc agtctactgt tcaaagagta ccactacaaa
     3061 atacaaaaag tagaccacca atcaaaaccg ccaccattaa gaaaggcagt aagaaaccat
     3121 gaagctgaaa actttagtga ttggtggttc gggattattc ttgatggtct tctcactgct
     3181 tctgtttgtc gccattttat tttcagatga acaagatggc ggtttttcca acattcacta
     3241 tggcggtgtg gatgtttccg cagaagtgct ggctcataag cctatggtag aaaaagttgc
     3301 caaagaatat ggaattgaag aatacgtcaa tatacttctt gcgattatac aggtggaatc
     3361 gggtggtaca gcagaagatg ttatgcagtc ctcggaatcc ctcggtcttc cacctaattc
     3421 attgagtaca gaagaatcca ttaagcaagg tgtgaagtat ttcagtgagt tattagccag
     3481 tagcgaaagg cttggtgtag atttagaatc ggttatccag tcctacaatt atggtggtgg
     3541 tttcttagga tatgtggcta atcgtgggaa taaatatacc tttgaactgg ctcaaagttt
     3601 ctcaaaagag tattcgggtg acgaaaaagt gtcttacccc aatcccatag ccatacctat
     3661 caatgggggc tggcgataca attacgggaa tatgttttat gtgcaactgg taacgcagta
     3721 tcttgtcaca acagagtttg atgatgatac agtacaagcc atcatggacg aagctctgaa
     3781 atttgaaggc tggaagtatg tctatggcgg agcttccccg actacctctt ttgattgtag
     3841 cggactgaca caatggacgt atggaaaagc tggaatcaac ttaccacgaa cagcacaaca
     3901 gcaatatgat gtaacccggc atatcccatt attggaagca aaagccggcg atttggtttt
     3961 ctttcattct acctataacg ctggctctta tattactcat atcgggattt atttaggcga
     4021 taaccgtatg tttcatgcag gcgaccctat cggttatgcc gacttaacaa gctcttactg
     4081 gcaacagcat ttagtgggag caggacgaat caaacaataa gaaaggacga ttcaatgatg
     4141 aaattcggaa aaaatcagaa tacagaaaaa cagacaccaa aggaaaagaa acctcgtgtc
     4201 tataaggtca atcctcgtaa aaaggttgtg attgtcctat gggtactttt aggacttagt
     4261 ttcagctttg cgatattcaa gcactttaca gccattgata cccataccat tcatgaaacc
     4321 accatcatag aaaaggaata tgtcgatact catcatgtgg aaaattttgt agagaacttt
     4381 gcgaaagtct actattcatg ggaactaaac gacaagtcca ttgataatcg catagaaaac
     4441 ctgaaagcat atctgacaga ggaacttcaa gctctgaatg tcgatacagt tcgtaaagat
     4501 attcctgtat cgtcttctgt aaaaggattt cagatatgga cggtagaagc agatggcgac
     4561 aatcaatttg atgtaaccta cagtgtagac caactcatta cagaggggga aaatacaaag
     4621 accgtccact ctgcttatat agtgagtgtc tatgtagaca gtactggaaa tatggtcctc
     4681 attaagaatc cgaccattac tagcatacca aagaaatcag actataaacc aaaagctatt
     4741 gaaagtgagg gtacggttga ttccattaca accaatgaaa tcaatgagtt tttaacgacg
     4801 ttcttcaagc tctatcctac agcgacagcc agtgaacttt cctactatgt gaatgacggg
     4861 atattaaaac caatcggaaa agagtacatc tttcaagaac tggtaaatcc tatttacaat
     4921 cgtaaggata atcaagtcac ggtatcgctg acagtggagt atatcgacca gcagaccaaa
     4981 gcaacgcagg tatctcaatt tgatttggta cttgaaaaga acgggagtaa ttggaagatt
     5041 atagaataac aaatattggt acattattac agctattttg taatcacgta ctctctttga
     5101 taaaaaattg gagattcctt tacaaatatg ctcttacgtg ctattattta agtatctatt
     5161 taaaaggagt taataaatat gcggcaaggt attcttaaat aaactgtcaa tttgatagtg
     5221 ggaacaaata attggatgtc cttttttagg agggcttagt tttttgcacc cagtttaaga
     5281 atacctttat catgtgattc taaagtatcc ggagaatatc tgtatgcttt gtatgcctat
     5341 ggttatgcat aaaaatccca gtgataagag tatttatcac tgggattttt atgccctttt
     5401 gggcttttga atggaggaaa atcacatgaa aattattaat attggagttt tagctcatgt
     5461 tgatgcggga aaaactacct taacagaaag cttattatat aacagtggag cgattacaga
     5521 attaggaagc gtggacaaag gtacaacgag gacggataat acgcttttag aacgtcagag
     5581 aggaattaca attcagacag gaataacctc ttttcagtgg gaaaatacga aggtgaacat
     5641 catagacacg ccaggacata tggatttctt agcagaagta tatcgttcat tatcagtttt
     5701 agatggggca attctactga tttctgcaaa agatggcgta caagcacaaa ctcgtatatt
     5761 atttcatgca cttaggaaaa tggggattcc cacaatcttt tttatcaata agattgacca
     5821 aaatggaatt gatttatcaa cggtttatca ggatattaaa gagaaacttt ctgccgaaat
     5881 tgtaatcaaa cagaaggtag aactgtatcc taatatgtgt gtgacgaact ttaccgaatc
     5941 tgaacaatgg gatacggtaa tagagggaaa cgatgacctt ttagagaaat atatgtccgg
     6001 taaatcatta gaagcattgg aactcgaaca agaggaaagc ataagatttc agaattgctc
     6061 cttgtaccct gtttatcatg gaagcgcaaa aagcaacata gggattgagc agcttataga
     6121 agtgataacg aataaatttt attcatcaac atacagaaag aagtctgaac tttgcggaaa
     6181 tgtcttcaaa attgaatatt cggaagaaag acaacgtctt gcatatgtac gcctttatgg
     6241 cggaatcctg catttgcggg attcggttag aatatcggaa aaggaaaaaa taaaaattac
     6301 agaaatgtat acttcaataa atggtgaatt atgtaaaatt gataaggctt attccgggga
     6361 aattgttatt ttgcaaaatg agtttttgaa gctaaatagt gttcttggag atacaaagct
     6421 attgccacag agagagagaa ttgaaaatcc gctccctctg ctgcaaacaa ctgttgaacc
     6481 gagcaaacct caacaaaggg aaatgttact tgatgcactt ttagaaatct ccgacagtga
     6541 cccgcttcta caatattatg tggattctac gacacatgaa attatacttt ctttcttagg
     6601 gaaagtacaa atggaagtga ttagtgcact gttgcaagaa aagtatcatg tggagataga
     6661 actaaaagag cctacagtca tttatatgga gagaccgtta aaaaatgcag aatataccat
     6721 tcacatcgaa gtgccgccaa atcctttctg ggcttccatt ggtttatctg tatcaccgct
     6781 tccgttggga agtggaatgc agtatgagag ctcggtttct cttggatact taaatcaatc
     6841 atttcaaaat gcagttatgg aagggatacg ctatggttgc gaacaaggat tatatggttg
     6901 gaatgtgacg gattgtaaaa tctgttttaa gtatggctta tatagccctg ttagtacccc
     6961 agcagatttt cggatgcttg ctcctattgt attggaacaa gtcttaaaaa aagctggaac
     7021 agaattgtta gagccatatc ttagttttaa aatttatgcg ccacaggaat atctttcacg
     7081 agcatacaac gatgctccta aatattgtgc gaacatcgta gacactcaat tgaaaaataa
     7141 tgaggtcatt cttagtggag aaatccctgc tcggtgtatt caagaatatc gtagtgattt
     7201 aactttcttt acaaatggac gtagtgtttg tttaacagag ttaaaagggt accatgttac
     7261 taccggtgaa cctgtttgcc agccccgtcg tccaaatagt cggatagata aagtacgata
     7321 tatgttcaat aaaataactt agtgtatttt atgttgttat ataaatatgg tttcttgtta
     7381 aataagatga aatatttttt aataaagatt tgaattaaag tgtaaaggag gaattaatta
     7441 tgaagtgtaa tgaatgtaac agggttaaat acaaccaaat tttaatttgg ctttgcgttt
     7501 tatctttttt tagcgtatta aatgaaatgg ttttgaacgt ctcattacct gatattgcaa
     7561 atgattttaa taaaccacct gcgagtacaa actgggtgaa cacagccttt atgttaacct
     7621 tttccattgg aacagctgta tatggaaagc tatctgatca attaggcatc aaaaggttac
     7681 tcctatttgg aattataata aattgtttcg ggtcggtaat tgggtttgtt ggccattctt
     7741 tcttttcctt acttattatg gctcgtttta ttcaaggggc tggtgcagct gcatttccag
     7801 cactcgtaat ggttgtagtt gcgcgctata ttccaaagga aaataggggt aaagcatttg
     7861 gtcttattgg atcgatagta gccatgggag aaggagtcgg tccagcgatt ggtggaatga
     7921 tagcccatta tattcattgg tcctatcttc tactcattcc tatgataaca attatcactg
     7981 ttccgtttct tatgaaatta ttaaagaaag aagtaaggat aaaaggtcat tttgatatca
     8041 aaggaattat actaatgtct gtaggcattg tattttttat gttgtttaca acatcatata
     8101 gcatttcttt tcttatcgtt agcgtgctgt cattcctgat atttgtaaaa catatcagga
     8161 aagtaacaga tccttttgtt gatcccggat tagggaaaaa tatacctttt atgattggag
     8221 ttctttgtgg gggaattata tttggaacag tagcagggtt tgtctctatg gttccttata
     8281 tgatgaaaga tgttcaccag ctaagtactg ccgaaatcgg aagtgtaatt attttccctg
     8341 gaacaatgag tgtcattatt ttcggctaca ttggtgggat acttgttgat agaagaggtc
     8401 ctttatacgt gttaaacatc ggagttacat ttctttctgt tagcttttta actgcttcct
     8461 ttcttttaga aacaacatca tggttcatga caattataat cgtatttgtt ttaggtgggc
     8521 tttcgttcac caaaacagtt atatcaacaa ttgtttcaag tagcttgaaa cagcaggaag
     8581 ctggtgctgg aatgagtttg cttaacttta ccagcttttt atcagaggga acaggtattg
     8641 caattgtagg tggtttatta tccataccct tacttgatca aaggttgtta cctatggaag
     8701 ttgatcagtc aacttatctg tatagtaatt tgttattact tttttcagga atcattgtca
     8761 ttagttggct ggttaccttg aatgtatata aacattctca aagggatttc taaatcgtta
     8821 agggatcaac tttgggagag agttcaaaat tgatcctttt tttataacag gaattcaaat
     8881 ctttttgttc cattaaaggg cgcgattgct gaataaaaga tacgagagac ctctcttgta
     8941 tcttttttat tttgagtggt tttgtccgtt acactagaaa accgaaagac aataaaaatt
     9001 ttattcttgc tgagtctggc tttcggtaag ctagacaaaa cggacaaaat aaaaattggc
     9061 aagggtttaa aggtggagat tttttgagtg atcttctcaa aaaatactac ctgtcccttg
     9121 ctgattttta aacgagcacg agagcaaaac ccccctttgc tgaggtggca gagggcaggt
     9181 ttttttgttt cttttttctc gtaaaaaaaa gaaaggtctt aaaggtttta tggttttggt
     9241 cggcactgcc gacagcctcg cagagcacac actttatgaa tataaagtat agtgtgttat
     9301 actttacttg gaagtggttg ccggaaagag cgaaaatgcc tcacatttgt gccacctaaa
     9361 aaggagcgat ttacatatga gttatgcagt ttgtagaatg caaaaagtga aatcagctgg
     9421 actaaaaggc atgcaatttc ataatcaaag agagcgaaaa agtagaacga atgatgatat
     9481 tgaccatgag cgaacacgtg aaaattatga tttgaaaaat gataaaaata ttgattacaa
     9541 cgaacgtgtc aaagaaatta ttgaatcaca aaaaacaggt acaagaaaaa cgaggaaaga
     9601 tgctgttctt gtaaatgagt tgctagtaac atctgaccga gatttttttg agcaactgga
     9661 tccaggagaa caaaaacgat tttttgagga aagttataaa ttattttccg aacgatatgg
     9721 caagcaaaat attgcttatg caacagttca taatgatgag caaacccctc acatgcattt
     9781 aggtgttgtg cctatgcgtg atggaaaact gcaaggaaaa aatgtgttta atcgtcaaga
     9841 actgttatgg ctacaagata aattccccga gcatatgaaa aaacagggtt ttgagttgaa
     9901 gcgtggtgaa cgtggctctg accgtaaaca tattgagaca gctaaattta aaaaacaaac
     9961 tttggaaaaa gagattgatt ttctagaaaa aaatttagca gttaaaaaag atgaatggac
    10021 tgcttatagc gataaagtta aatcagattt agaagtacca gcgaaacgac acatgaaaag
    10081 tgttgaagtg ccaacgggtg aaaagtccat gtttggtttg ggaaaagaaa taatgaaaac
    10141 agaaaagaaa ccaaccaaaa atgttgttat atcggagcgt gattataaaa acttagtgac
    10201 tgctgcgaga gataacgata ggttaaaaca gcatgttaga aatctcatga gtactgatat
    10261 ggcgagagaa tataaaaaat taagtaaaga acatgggcaa gttaaagaaa aatatagtgg
    10321 tcttgtagag cgatttaatg aaaatgtaaa tgattataat gagttgcttg aagaaaacaa
    10381 gtctttaaag tctaaaataa gcgatttaaa gcgtgatgtg agtttaatct atgaaagcac
    10441 taaggaattc cttaaggaac gtacagacgg cttaaaagcc tttaaaaacg tttttaaggg
    10501 gtttgtagac aaggtaaagg ataaaacagc acaattccaa gaaaaacacg atttagaacc
    10561 taaaaagaac gaatttgaac taactcataa ccgagaggta aaaaaagaac gaagtcgaga
    10621 tcagggaatg agtttataaa ataaaaaaag cacctgaaaa ggtgtctttt tttgatggtt
    10681 ttgaacttgt tctttcttat cttgatatat atagaaataa cgtcattttt attttagttg
    10741 ctgaaaggtg cgttgaagtg ttggtatgta tgtgttttaa agtattgaaa acccttaaaa
    10801 ttggttgcac agaaaaaccc catctgttaa agttataagt gaccaaacaa ataactaaat
    10861 agatgggggt ttcttttaat attatgtgtc ctaatagtag catttattca gatgaaaaat
    10921 caagggtttt agtggacaag acaaaaagtg gaaaagtgag accatggaga gaaaagaaaa
    10981 tcgctaatgt tgattacttt gaacttctgc atattcttga atttaaaaag gctgaaagag
    11041 taaaagattg tgctgaaata ttagagtata aacaaaatcg tgaaacaggc gaaagaaagt
    11101 tgtatcgagt gtggttttgt aaatccaggc tttgtccaat gtgcaactgg aggagagcaa
    11161 tgaaacatgg cattcagtca caaaaggttg ttgctgaagt tattaaacaa aagccaacag
    11221 ttcgttggtt gtttctcaca ttaacagtta aaaatgttta tgatggcgaa gaattaaata
    11281 agagtttgtc agatatggct caaggatttc gccgaatgat gcaatataaa aaaattaata
    11341 aa
//
LOCUS       DACKAC010000083        11321 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.084, whole genome shotgun sequence.
ACCESSION   DACKAC010000083 DACKAC010000000
VERSION     DACKAC010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.084"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(40..1419)
                     /locus_tag="I0B46_002387"
     CDS             complement(40..1419)
                     /locus_tag="I0B46_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HAR2084644.1"
                     /translation="MTKVTLKKVNPPKKDNEAKIYQLTDETMKFRKEKVLAAMEKSEI
                     DTLVIYCDLEHGGNFSYLTGFVTRFEESLLVLHQTGEAFLVLGNENTKMVDYSRIEAE
                     LIHTPLFSLPDQPMDNEKPLTEVLTNAKISKGKKVGLVGWKMFTSEEDNYQIQDLPAF
                     IVSSIVELVGEDAVSNRTDLFIHSDYGVRSLNTANEVAYYEFGSSLASDCVMNAIDEV
                     EVGKTEMEIGNHLVKYGQPNNVIPIAATGERFENAYIYPTDKQVKLGDKMSITTGFKG
                     GLASRSGYAVATTDDLPEGQKDYIEKVAAPYYDAVVAWLENIKIGMQGKELYSVIGKV
                     LPKSEYKWHLNPGHLTADEEWMSSPMKKDSEITLKSGMLLQIDIIPSVAGYAGASCES
                     GIALADESLRDQLAKEYPNVWSRIISRQEYIRNELGIELPDEVLPLSSGVGYYTPFFL
                     EKNLALVKG"
     gene            complement(1440..2987)
                     /locus_tag="I0B46_002388"
     CDS             complement(1440..2987)
                     /locus_tag="I0B46_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAR2084645.1"
                     /translation="MGPLSIIVASGLLLGIVSILQNPTLVGESFANATVIQGFVGGVQ
                     AIVGTMFGLLPILFAISVAIGMSREDKEIAAFSVVIAFILFHVVINYLLQLRGITAES
                     TSVETLMSDGMSQIKAVETSNMYETLLGTFTYRMGVFGGIIAGLWTAMIHNRYHTKQL
                     PAAFSFFSGNRFVPIMIILTIPIISIASFFIWPFVNTAINALGGLIGKSGVIGTFLYG
                     FSERLLIPTGLHHVLNQLIRFTPIGGTATINGETVSGALTIFNTELALPVKDMDILRS
                     ATRFLTQGTHPFMVFGLPAACYAMYKTSYLAQRPKIKGMLIAAAATCFVTGITEPIEF
                     AFIFISPILWVFHAFMAGLSFMLMNLFGVAVGNAGGGFIDLAIFGILQGTYTRWYLVV
                     ALGLVYAVAYYFVFKYVIERFDVKTPGRSNDDVLEDQDEEMTENELGKLILQGLGGAG
                     NIQEIDNCISRLRLVLKDTATIDEHILKKTGSMGIVKINENNLQVVYGGQVENATRSL
                     KNEMKRK"
     gene            complement(3236..4015)
                     /locus_tag="I0B46_002389"
     CDS             complement(3236..4015)
                     /locus_tag="I0B46_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAR2084646.1"
                     /translation="MTLEQLVNEHHSKLNENDDYVLSFITQNMEISISQTVSELAKQA
                     NVSDSTIIRLTKKLGFKGYGEFRYYLKEEFARMQKTRVDKSSLFLPEVLLGDVQATTK
                     LFEDDKSVEDIYETIRKADKIFAYATGYGQRMMLNEFARCMWNLGIYIIVVPEKNELD
                     LISKALTSNDLLFVISLSGRVDRIDDTLRRIHMKGTQLVSVTRFGQNNLASLSDYSLY
                     YQVTNINDVNELNNSSFCTLNLALSLLYEGYINYYKQSSVD"
     gene            complement(4090..4548)
                     /locus_tag="I0B46_002390"
     CDS             complement(4090..4548)
                     /locus_tag="I0B46_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAR2084647.1"
                     /translation="MGFFSRKEKLVAPVSGNLIPLSQVSDPVFSQGMMGEGFAVEPTN
                     AKVVAPIDGVIKSIFPTKHALTLTSNQGRDLLIHIGIDTVQLKGVGFQVLVEEGQRVS
                     SGESLVIFDCDQIKAEKLSDVVMVLFPEDKGIKLEVSEKKVSEGELLFKI"
     gene            complement(4560..5819)
                     /locus_tag="I0B46_002391"
     CDS             complement(4560..5819)
                     /locus_tag="I0B46_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HAR2084648.1"
                     /translation="MKTNFERIKKDIENLAEYNATPGEGVTRSAYSKEDREARNYLIN
                     QMEQLGLEIWEDGFSTLFGRRAGKKADAPSVMIGSHYDSVVNGGRFDGVAGVVTALET
                     MRILEENNFENDYPIDLILMNAEEGETFGPSTGVSNSRAMMGTLTMSELETVKNRHGQ
                     TKLEALRAYGVEPDLEAAKRDPKTIKNFIELHIEQGPVLEDEGKDIGLIEFIPGIGRF
                     KARFYGETEDSTAPMNKRKDALVAASKFVLFVEQWMKNLGEGIAGTVGKLTIKPNSNQ
                     FVPDFVEALIEIRTFDTKTTNPQEIKEKLLNELSVIQGETGVRTELEEMARINYSNPT
                     NPSVMDQGNVEKMKHICDQLGYSYQVINNGTGHDAMMMTDFVPTNMIYIPSKGGITHH
                     PDEWTDFEDVKKGADVLLNLVMDISKD"
     gene            complement(5935..6249)
                     /locus_tag="I0B46_002392"
     CDS             complement(5935..6249)
                     /locus_tag="I0B46_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAR2084649.1"
                     /translation="MDYEIQKFISEKEDQYFDRKSARIKPRDIIKHIVAFANADGGKL
                     VIGIEDDGTISGFKFQGAIDPWICVNKVDAFIESFLNQSTLELCYETRKSSYGGIYDN
                     DF"
     gene            complement(6395..7891)
                     /locus_tag="I0B46_002393"
     CDS             complement(6395..7891)
                     /locus_tag="I0B46_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HAR2084650.1"
                     /translation="MNHQEAKQFFLKTSSFFSTSLPNYIDLSVGIEKAVDLLKNKPFT
                     ELVRHKRSLSEAVDVNHRILANKDGNYAWRPLQILHPLVYVDLVNGITQKNNWNHIKN
                     KFEEFQADKRIECISLPVESISSKSDTAETILNWWENLEQAQISYALEYDYCIHSDIT
                     DCYSSMYTHTIPWAVHSKEWAKTHRKDTQGVGNLIDSKIRYLQNGQTNGIPQGNVLMD
                     FVAEIVLGYADKMLLNKIEKTEIKEFKILRYRDDYRIFSVRKDQAEVIIRLLSEVLSD
                     LNLKLNSNKTFLSDNIILDAIKPDKIYWDLKHASFTEKSNNKWKFKLSLQKHLLQVKL
                     LGDKYPNCGSLSKALSEVYTHRVSTLNKRPDDIYQLISILVNIMQKNPRTLEACIVIL
                     GKLFEFIAVEEINLYLDKILRKFVNTPNTDIVEIWLQRLSLIHSREKSYSAKLCKKVS
                     NPKGFTLWNSDWLKKGFDESGIVNETFISNLSLTTPAKDLELFISQYE"
     gene            complement(8069..8548)
                     /locus_tag="I0B46_002394"
     CDS             complement(8069..8548)
                     /locus_tag="I0B46_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HAR2084651.1"
                     /translation="MKVYTVQPVDFLQSVDSEGFIYPKKRYTELSVFFDNPNAVGALH
                     WLEEEYTKRIQLPLDRDLIWLWRDTCHLKYARKDNFTDFSKYNFYCFEIPIEFYRKNI
                     LWSRFIEWHEPLNHEWETDENQEFEHIFDIPKHPGYGDIQGVTTRLHKDWIKKITHN"
     gene            complement(8924..9169)
                     /locus_tag="I0B46_002395"
     CDS             complement(8924..9169)
                     /locus_tag="I0B46_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAR2084652.1"
                     /translation="MLFLKEEKLIDKENVIGSNIRRIRLEKGMGQTELVRELQLSKIA
                     MTRETLVKIEGGRQHIKLDQLRAIRDILQVSYEDLLQ"
     gene            9274..10047
                     /locus_tag="I0B46_002396"
     CDS             9274..10047
                     /locus_tag="I0B46_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084653.1"
                     /translation="MKKFAIILTCALLTGAISGCATSDTNSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLDVPLSESNSIEKVLPIEIEKNQENIKLKAELPILNSTEIDW
                     HENIKVTIEKGALAQLYTEKVEQFFESGANKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMTLKKIKAYDHPLNLTLNNGLLSFVLAGYVDYEYSPDYLETPLQHENNDINFR
                     FVYESKSKTWLVDQLSTINTSFHELPKPENEADMVIKIF"
     gene            complement(10276..10524)
                     /locus_tag="I0B46_002397"
     CDS             complement(10276..10524)
                     /locus_tag="I0B46_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAR2084654.1"
                     /translation="MDTITPTNFKKNIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSDLMKNEKEDDFKVV"
     gene            complement(10623..10937)
                     /locus_tag="I0B46_002398"
     CDS             complement(10623..10937)
                     /locus_tag="I0B46_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084655.1"
                     /translation="MADENKVVPLETLVINKEEFEQLFLDFFVQYDRIYDFSSSLDLF
                     ELEAEIIENSREENGKIIISVEKFNELFSETFEIDTDYYQPQEVFRLFRDQIIGISQT
                     TK"
BASE COUNT         3515 a         2326 c         1697 g         3783 t
ORIGIN      
        1 tcatttcttc cgttttaaag aagaaatgat tttcatagat caacctttaa ctaaagctag
       61 attcttttct aaaaagaatg gtgtgtagta gcccacaccg ctggacaacg gtaaaacttc
      121 atccggcaac tcgatgccca gttcgttacg gatgtactct tggcgagaaa tgattcgcga
      181 ccaaacattt ggatattctt tggctagttg atcacgaagc gactcatcag ctagcgcaat
      241 cccactctca cagcttgcgc cggcatagcc agcaactgat ggaataatat caatttgtag
      301 taacattccg gacttaagag taatttctga gtcttttttc attggcgaag acatccactc
      361 ttcatcagcc gtaagatgcc ccggatttaa gtgccatttg tattccgatt taggcaagac
      421 tttgcctatt actgaatata gctctttacc ttgcattcct attttgatat tttccaacca
      481 tgcgaccacg gcgtcgtagt atggagctgc aactttttca atgtaatcct tttgcccttc
      541 tggtaggtca tctgtcgttg caactgcata gccacttcga cttgctaatc cgcctttaaa
      601 gcccgtagta attgacattt tatcgcctag tttaacttgt ttatcagttg gatatatata
      661 ggcgttctca aaccgttccc cagttgcagc aattggaatg acattatttg gttggccgta
      721 cttaactaag tggttaccaa tttccatttc tgtttttcct acctctactt catcaatagc
      781 gttcatcaca caatctgaag ccaaggatga gccaaattca taatatgcca cttcattagc
      841 agtatttaat gagcgtactc cgtaatcact gtgaatgaac aggtcggtgc gattgctaac
      901 ggcatcttcc cctactaatt ctacaatcga acttacaatg aatgccggca aatcttggat
      961 ttggtagtta tcctcttctg aagtaaacat cttccaacca acaagcccaa ctttcttacc
     1021 tttactgatt ttagcattgg tcaatacttc agttagtggt ttctcgttat ccatcggttg
     1081 atccggtaat gagaagagtg gcgtgtggat taattctgcc tcaattcttg aatagtccac
     1141 cattttagta ttttcatttc ctagaactaa gaaagcttca cccgtctgat gtaataccaa
     1201 caagctttct tcaaagcgag taacaaaacc tgttaaataa gagaagttcc cgccatgttc
     1261 caaatcacaa tagattacga gggtatctat ctctgacttt tccatcgctg caagtacctt
     1321 ttctttacgg aacttcattg tttcatcagt caattgatat attttagctt cattatcttt
     1381 tttaggtggg ttaacttttt ttagagttac cttagtcata ttgtcactac tttctgtttt
     1441 tatttacgct tcatttcatt tttcagagat cgagttgcgt tttccacttg tcctccatat
     1501 accacttgta aattattttc gttaatttta acgattccca ttgagccggt ctttttcaag
     1561 atatgttcat caattgttgc tgtatcttta agaactagtc tcagccgaga gatacagtta
     1621 tcgatttctt gaatattacc agcaccacca agtccttgca agattagttt gccaagttcg
     1681 ttttctgtca tttcttcgtc ctgatcttcc aacacatcat cgttggatcg accaggagtt
     1741 ttcacatcaa atcgctcaat gacatactta aatacaaagt aatatgcaac tgcataaaca
     1801 aggcccaatg ctacaactaa gtaccaacga gtataagtac cttgtaaaat tccgaagata
     1861 gccaaatcaa taaatccacc accggcattc ccaactgcga ctccgaatag attcattaac
     1921 ataaaggata aaccagccat gaatgcatgg aagacccata gaatcggtga gataaagata
     1981 aaggcgaatt caattggctc agtaatcccg gttacaaaac aagtagccgc tgctgcaatc
     2041 aacatccctt taatctttgg acgttgcgcc aaatatgaag ttttgtacat tgcatagcat
     2101 gctgccggca atccgaagac cataaatgga tgagtacctt gtgtcaagaa gcgagtagca
     2161 cttctcaaaa tatccatgtc ttttactggc aatgctaatt cagtattgaa gatggttaat
     2221 gctccagaaa ctgtttcacc attgatagtt gccgtaccgc cgattggtgt aaatcgaatc
     2281 aactggttta gtacatggtg aagacctgtt ggaatcagca ggcgctctga gaagccatat
     2341 aaaaaagttc caattacgcc tgacttacca atcaaaccgc ctaaagcatt gatagctgta
     2401 ttgacaaaag gccagatgaa aaatgaagcg attgagatga ttggaatagt taggatgatc
     2461 ataattggaa caaaacgatt gccactaaag aaactgaaag ctgctggtaa ttgttttgtg
     2521 tgatagcgat tgtggatcat ggctgtccat aatcctgcaa tgataccgcc gaaaacaccc
     2581 atacgatatg tgaatgtgcc aagtaaagtt tcatacatat tggaagtttc tactgctttg
     2641 atttgactca ttccgtcaga catcaatgtt tcaacagaag tgctttccgc tgtgatgcct
     2701 ctaagctgaa gcagataatt gataactaca tggaacagaa taaatgcaat aaccactgaa
     2761 aatgctgcaa tttccttatc ttctctagac atcccgattg ctactgaaat agcaaataga
     2821 ataggaagta gtccaaacat cgtaccaaca atagcctgta ctccaccaac aaatccttgg
     2881 atcacggttg cattagcgaa gctttctccc accaaagtag gattttgtaa aattgaaacg
     2941 attcctagta gtagtcccga tgcgacaata atagacaacg gacccattaa tgatttccct
     3001 aacttctgga tataagttct caatataaac accttcttct ttttagttga tataaacgtt
     3061 tacatccctt gtacaacgaa agtatagcac cctgaaaaag cgctaacaat acgataggtc
     3121 tgataaaaat caacattcag cacttggttg ttcattcaaa acgtgaatct tcaatcacag
     3181 aaaaatacgc gctatcaagc acgtatttta ggtgtaaata tatgcatcaa ctttattaat
     3241 caacgctact ttgtttgtaa tagttaatat acccttcata cagtaagcta agagcaagat
     3301 tcaaggtgca gaatgatgag ttgttcaact cattcacatc attaatgttg gtcacttgat
     3361 agtataggct atagtctgac agactggcta agttattttg accaaatcgt gtaactgaga
     3421 ccaactgtgt ccctttcata tggattcttc ttaaagtatc atcaatccga tcaacacgtc
     3481 cggacaatga tatgacaaat agtaaatcat tcgaggtcaa agcttttgaa atcaggtcta
     3541 attcattctt ttccggtaca acgataatgt agattcccaa gttccacatg cagcgtgcaa
     3601 actcattgag catcattctt tgtccatatc ctgttgcgta ggcaaatatc ttatctgcct
     3661 ttctaatcgt ttcatatata tcctcaacac ttttatcatc ttcaaacaat ttggttgtcg
     3721 cttgaacatc tccgagtaat acttccggta aaaacaaact acttttatcg actctggttt
     3781 tttgcatgcg tgcaaattcc tctttaaggt aatatcgaaa ttcgccatat cctttgaatc
     3841 cgagtttctt agttagtcta ataatcgttg agtcagacac atttgcttgt tttgctaatt
     3901 ccgatacagt ttgactgatg ctgatttcca tattttgagt gataaaactt aatacataat
     3961 catcattctc attcaattta ctgtgatgtt catttactaa ttgctccaat gtcataatca
     4021 gacctccaaa aataataaaa aagattgctc tctcatttta atacgaaagc acaatctttt
     4081 taagtactat taaattttga aaagaagttc tccttcagaa accttttttt cagagacttc
     4141 tagtttaata cccttatctt cgggaaatag aaccataaca acatctgaca gtttttccgc
     4201 cttgatttgg tcacaatcaa aaatcaccaa tgactcgccg gatgatactc gttgaccttc
     4261 ttcaacgagc acttgaaacc cgactccctt taactgaaca gtatcaattc caatatgaat
     4321 caataaatct ctaccttgat tactagtcaa tgtgagtgca tgctttgttg gaaatatcga
     4381 tttaatgaca ccgtcaatcg gtgctaccac cttagcgttt gttggttcaa cagcgaatcc
     4441 ttcgcccatc atcccttgcg aaaatacagg atcgcttact tggctaaggg gaatcagatt
     4501 tcctgatact ggagcaacta atttttcttt tcgactaaaa aatcccattg acttactcct
     4561 taatcttttg aaatgtccat cacaaggttt aaaagaacat ccgccccttt tttgacatcc
     4621 tcaaaatcag tccactcatc aggatgatgt gtaatgccac ctttggatgg aatataaatc
     4681 atatttgttg ggacaaagtc ggtcatcatc atcgcatcat gtccagtacc attgttaatg
     4741 acttgatagc tatagcccaa ctgatcgcaa atatgtttca tcttctcaac attaccttga
     4801 tccataacac ttggatttgt aggattactg tagttaattc gagccatttc ctccagctca
     4861 gttctaactc cagtttcccc ttgaatcact gataactcat tcaacaactt ttctttaatc
     4921 tcttgaggat tagttgtctt tgtatcaaag gttctgattt cgattaaagc ttcgacaaag
     4981 tcagggacaa attgatttga gttcggtttg attgtaagtt taccgacggt tccggcaatc
     5041 ccttcaccaa gattcttcat ccactgttca acaaaaagaa caaatttaga agcagcaact
     5101 aaagcatctt tccgtttatt cattggagca gtcgagtcct cagtttctcc ataaaaacgt
     5161 gctttaaaac ggccgatgcc aggaataaat tcaatcaatc cgatatcttt gccttcatct
     5221 tccaggactg gtccttgttc gatatgaagt tcaataaaat ttttgattgt ttttggatct
     5281 cgcttagcgg cctctaaatc tggttccact ccataagcac gaagagcttc caattttgtt
     5341 tgtccatggc gattttttac tgtttccaac tcactcatgg ttaatgttcc catcatagct
     5401 cgtgaatttg agacacccgt acttggccca aatgtctcgc cctcctctgc attcatcaga
     5461 attaggtcaa ttgggtaatc attttcaaaa ttattttctt ccagaatccg cattgtttcc
     5521 agtgcagtaa caacgccagc cactccgtca aatctgcctc cattaacaac tgaatcataa
     5581 tgagagccaa tcatcactga tggtgcatca gcctttttac cggctctacg accaaataat
     5641 gtagagaagc cgtcttccca aatctctaaa cccagttgtt ccatttgatt gatcaaataa
     5701 tttcttgctt cacgatcttc tttagagtaa gcagagcggg tcactccttc acctggagtt
     5761 gcattgtact cggctaaatt ttcgatatcc ttttttatac gctcaaaatt tgtcttcata
     5821 attaaatcct ccatttccta caagtgaatg ttagcaccca tagaaagcgg ctacaatata
     5881 atcaatgaag attgaatcga tattcgaaaa ctgaatattg cttcggttaa tgacttagaa
     5941 atcattatcg tagattccac cataagaact ttttctcgtc tcataacaca actcgagagt
     6001 ggattggttt aaaaaactct cgataaatgc gtccacttta ttgacacaga tccatggatc
     6061 gatcgcacct tgaaatttaa acccagaaat agtcccatca tcttctatac cgattaccag
     6121 cttacctcca tcggcatttg caaacgctac tatgtgtttt ataatatctc taggcttaat
     6181 ccgtgcactt tttctatcaa aatactgatc ttctttttca cttataaact tttgaatctc
     6241 ataatccatt taaaatttcc cttcttaaat ttttttatta tagagccgta tattcaattt
     6301 ccacaaaaaa ataatctaaa acaaaacaga caccttcatt ttgatatgtt ctcccacata
     6361 tgaatcggtc gctatacagc attacatata gtaattattc atactgcgaa ataaatagct
     6421 ccaaatcttt agctggcgta gttaagctta aattcgaaat gaaagtttca ttgactattc
     6481 cactttcatc aaatcccttc ttcaaccaat cagagttcca caacgtaaat cctttaggat
     6541 tagaaacttt cttacataat tttgcactat aggatttctc tcttgaatga attaaagaaa
     6601 gtctttgtaa ccaaatttca actatatccg tattaggagt attaacaaat tttcttaaga
     6661 ttttatccaa gtataagttg atttcttcta cagcaataaa ctcaaaaagc ttacctaaaa
     6721 tcacaataca agcctccaaa gttctaggat tcttttgcat tatattaaca agaatactaa
     6781 tcaattggta gatatcatct ggtcttttgt ttaaagtaga aactctatgt gtatagactt
     6841 cacttagcgc tttacttaaa ctcccgcaat ttggatattt gtctcctaaa agctttacct
     6901 gtagcaaatg cttttgaagg ctaagcttaa acttccactt attattactt ttctctgtaa
     6961 aacttgcatg tttaagatcc cagtaaattt tatcaggctt aattgcatct aaaattatat
     7021 tatccgataa gaatgtttta tttgaattta gcttcaaatt tagatccgat aatacttcag
     7081 ataacaatct gataatcact tcagcttgat cttttcgaac cgaaaaaatt ctgtaatcat
     7141 ctctgtaacg aagtattttg aactctttaa tctctgtttt ttctatcttg ttcagtaaca
     7201 ttttatcagc atacccaagt actatttcag ctacaaaatc catcaataca tttccttgag
     7261 gtataccgtt cgtttgtcca ttttgtaaat atcgtatttt agaatctatt aagtttccaa
     7321 cgccttgagt atcttttcta tgtgtcttag cccattcttt tgaatgtact gcccacggga
     7381 tagtatgtgt gtacattgag ctataacaat cggtaatatc tgaatgaatg cagtagtcat
     7441 actccaaagc ataactaatc tgtgcttgtt caaggttctc ccaccaattt aaaatagttt
     7501 ccgcagtatc gctctttgaa gaaatagatt ccacaggaag tgatatacat tcaattcgct
     7561 tgtcggcttg gaattcttca aacttatttt tgatgtgatt ccaattattt ttctgtgtaa
     7621 tcccgtttac taagtctaca tatactaatg gatgtaatat ttgaaggggt ctccaagcat
     7681 aattaccatc tttattagct aatattcgat gattaacatc aactgcctca gagagcgatc
     7741 tcttatgtct aactagttca gtaaatggtt tgttttttaa taaatccact gctttctcaa
     7801 tgcctactga taaatcaata tagttgggca acgatgtact aaaaaaactt gatgttttta
     7861 aaaagaattg ctttgcttct tgatgattca tctctaaaat actttttcta gtcctagtca
     7921 aattcaaaat ctcctagatc aattaagttg taatgtaaga ggatacccta aggttacaca
     7981 ccctatttac tattatacca gtattcattc gtgatacata agggtgtaac attaagaact
     8041 ctatctaaaa gactacaagt agtcaccttt aattgtgagt aattttcttt atccaatcct
     8101 tgtgcaacct tgtcgtcacc ccttgtatat ccccgtaccc cggatgtttc ggaatatcaa
     8161 aaatgtgctc aaattcctga ttttcatcag tctcccactc atgattcaac ggttcatgcc
     8221 actctataaa ccttgaccat aaaatgttct ttctataaaa ctcaatagga atttcaaaac
     8281 agtaaaaatt gtatttgcta aaatctgtaa aattatcttt acgagcatac tttaaatggc
     8341 aggtatctcg ccacaaccaa attaaatctc gatccaatgg taactgaatt cgtttcgtat
     8401 actcttcttc aagccaatga agtgcaccaa ctgcattcgg attatcgaaa aagacagata
     8461 attcagtata gcgtttcttt ggataaataa atccttctga atcaacagat tgcaaaaaat
     8521 ctactggttg aaccgtgtac actttcattt attcgccacc tttttttgac tacttatagt
     8581 catttaccaa tttgaaatct gcatactttt tcattcaatt gagcccagca ttaattgtac
     8641 acgagttttc ttcactaacg gatagtatca ggatatacta atactatcct gaatagcgaa
     8701 atgaggtagc caaaatgaga aaagtcgatg aatatatcaa aaagaaatcc caaaaaagta
     8761 aagtatggca aagaacattt gagttagagt ataagaaact ctcagatagc ctcaaaaagg
     8821 acacttataa tccaaatgaa caaagtaaag cagataacca ataaagatta ccctcccata
     8881 tttaacattg cttcacgaat cagtaatcaa tgactacaag tagtcattga agcaaatctt
     8941 cataagatac ctgtagaata tccctaatag ctctcaattg atccagctta atatgttgcc
     9001 ggccaccttc aatcttaacc aatgtttctc ttgtcatagc gattttactg agctggagtt
     9061 ctcgcaccag ttctgtctgt cccattccct tttctaatcg gattcgtctg atattgctac
     9121 cgattacatt ttctttatct attaactttt cttccttcaa aaatagcacg ccttttggac
     9181 taaaattagt cctttttctt attaatctta aagatttggt atgctacaat gggactagta
     9241 ttagtccata ttcaatttta aggaggaatt tttttgaaga aatttgcaat cattctaacc
     9301 tgtgctttac tcacgggagc tataagtggg tgcgccactt ctgacacaaa ttctacaagt
     9361 actgaaacaa gtacaacaac ctcagaaaag aattatccta tacatttttt tccagttcca
     9421 gatggatttg aagcttatta tcatgataaa aaactagatg ttccattgtc agaaagtaac
     9481 tctattgaaa aagttttgcc aattgaaatt gaaaaaaatc aagagaatat aaaactcaag
     9541 gccgagcttc ccatactgaa cagcacagaa attgattggc acgagaatat taaagtaact
     9601 attgaaaaag gagcgttagc acaactttat actgaaaaag ttgaacaatt ttttgagtct
     9661 ggtgccaata aagacttttc aaaaataagc gattattcgg ataacttttt taatgagacc
     9721 caagatgaaa tcacgaactc aacttttatg gctgaagata tgtacaatat gacgttaaaa
     9781 aaaataaaag cttacgacca tccattaaat ctgacactaa ataatggatt actctctttt
     9841 gtattagcag gttacgttga ctatgaatat tcaccagatt atttggagac acctcttcaa
     9901 cacgagaaca atgatattaa ttttagattt gtctacgagt caaaaagtaa aacatggtta
     9961 gttgatcaat taagcactat aaacacaagc ttccatgaat tacctaaacc tgaaaatgaa
    10021 gctgatatgg tcattaaaat cttctaaaaa agaagcgttg aaggagaaat attcctttca
    10081 acgctctttt ttatatgctt agaaacataa ataaattgta tactttcttt ttagccccta
    10141 ttacttttca cgaaaaatat cttttttcga cactagcgtt tctctactta taatattgta
    10201 cacagttttt gtattattga aaaattactc attatttagc attaatccac atcgttttat
    10261 caatcgacct tcatactaaa ctactttaaa gtcatcttcc ttctcatttt tcattaaatc
    10321 agatacataa gcccatgtac ccgttcgttg taaataaagt tcttcttgta gcagctccca
    10381 ctttctctta gaaatcataa tgacgccatc gttcaatcca tttttagaat ccctagtaat
    10441 ttctatttca gaatttgaat gaactacttc ttgcaacact ttacctatat tttttttgaa
    10501 gtttgttgga gtaatagtat ccaaaatgaa cacctctctt ctctatacct ctattatgaa
    10561 gcgcccatca aataaagcaa gcgttgagga ataattcttt caacgcttgc tttattaagc
    10621 tttcattttg tagtttgact aataccaata atctgatctc taaacaatcg aaaaacttct
    10681 tgtggttgat aataatctgt atctatttca aatgtttctg agaacaattc attgaatttt
    10741 tctactgaaa tgatgatttt accattctcc tctcgactgt tttctattat ttctgcttct
    10801 aattcaaata aatctagtga cgaactaaaa tcataaatcc tgtcatattg aacaaaaaaa
    10861 tcaagaaaaa gttgctcaaa ctcttcctta tttatcacta gtgtttcaag cggaacaact
    10921 ttattttcat cagccattct tccaatttcc tcactttcat tttcaaaaaa tcgtaaaatt
    10981 gatatagatt ctttgtctct aatcactcgt gatattatct tgacagtcct acatctaatt
    11041 caattaaagt ataagccact ttcaaataaa agattacaaa gtaaatggca agccaacaaa
    11101 ataaccatct gcttcacgta ttacgatttg ataattctgg atttcgctcg gaagttcata
    11161 ataataatta acagtttctg ctttgccact ttcaatatat tctggattat tcaatatcat
    11221 agcatactca taaagttctt caccattttt atttggttct ataatatttt gtgttggtgg
    11281 aaaatcgaag gcgattttct actaacactt tttcttcgtt t
//
LOCUS       DACKAC010000084        11254 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.085, whole genome shotgun sequence.
ACCESSION   DACKAC010000084 DACKAC010000000
VERSION     DACKAC010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.085"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="I0B46_002399"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="I0B46_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAR2084656.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="I0B46_002400"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="I0B46_002400"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAR2084657.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="I0B46_002401"
     CDS             complement(2169..2906)
                     /locus_tag="I0B46_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAR2084658.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="I0B46_002402"
     CDS             complement(2997..3524)
                     /locus_tag="I0B46_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAR2084659.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="I0B46_002403"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="I0B46_002403"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAR2084660.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="I0B46_002404"
     CDS             complement(4401..4886)
                     /locus_tag="I0B46_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAR2084661.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="I0B46_002405"
     CDS             complement(5039..5236)
                     /locus_tag="I0B46_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084662.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="I0B46_002406"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="I0B46_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAR2084663.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="I0B46_002407"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="I0B46_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAR2084664.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9211..9423
                     /locus_tag="I0B46_002408"
     CDS             9211..9423
                     /locus_tag="I0B46_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAR2084665.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9425..11104
                     /locus_tag="I0B46_002409"
     CDS             9425..11104
                     /locus_tag="I0B46_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAR2084666.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
BASE COUNT         3265 a         2429 c         1971 g         3589 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata
     9001 acaattaatt gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga
     9061 taagcaaaaa cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga
     9121 aaaaccaggt ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca
     9181 ctgctttacg cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca
     9241 aaagtacgca accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat
     9301 gtagacgctc gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt
     9361 ctagaaggaa gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt
     9421 taatatgaaa ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg
     9481 tgaaatcggg aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc
     9541 cgggatcaaa ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag
     9601 ctacatcata caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga
     9661 ccacatcggc ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc
     9721 tctcgcatta gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga
     9781 gctccatgag atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt
     9841 cagaacgacc catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa
     9901 tatcgtagcg actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt
     9961 gcataaaatg gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa
    10021 tgctgagatt ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat
    10081 cttttcaaaa atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt
    10141 gcagcaagca gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag
    10201 catggaaaat gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac
    10261 atttgtagat gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac
    10321 aggttctcaa ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca
    10381 aatccaaatc cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac
    10441 aacaagtgtc aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg
    10501 aaaaatcaat aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct
    10561 tcgtttgatg aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt
    10621 acatgcttct ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa
    10681 tggcgatgtt ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg
    10741 agatgtgtat atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg
    10801 tcacctgctt tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa
    10861 agaaatcttg gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg
    10921 agaaatgatc aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg
    10981 acctgactgt tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt
    11041 attcgaacgt acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga
    11101 ataaataaga ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa
    11161 acatgagaag ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc
    11221 tcttaaattt atttactttt tcatacaaaa aaat
//
LOCUS       DACKAC010000085        11240 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.086, whole genome shotgun sequence.
ACCESSION   DACKAC010000085 DACKAC010000000
VERSION     DACKAC010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11240
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.086"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(368..829)
                     /locus_tag="I0B46_002410"
     CDS             complement(368..829)
                     /locus_tag="I0B46_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAR2084667.1"
                     /translation="MEDLGSKLRELRVKKGYTQEYVARKFNVTRQTISKWENNKTTPD
                     IETLKKICEFYSTNINSILNEDNLKNKQNNLLVLTAIIFNIFVTILGLFELIQMNFFS
                     FTHGIFTWFALSGLIVIDILNYFYIYYSYLKNEKFFYTIFFPLVILIILLS"
     gene            962..1336
                     /locus_tag="I0B46_002411"
     CDS             962..1336
                     /locus_tag="I0B46_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HAR2084668.1"
                     /translation="MKKFLCLSALMGVLLTSGGIVSATEALNLDVPEDHTAIYGGGME
                     ATEEGFSSSKLRYAAGGGDFNCGVNGFKVYANYYHARAKHSATAKNGRGGQVRSVQKA
                     GVRAYATCNATLTGNTGWWNVY"
     gene            1390..3516
                     /locus_tag="I0B46_002412"
     CDS             1390..3516
                     /locus_tag="I0B46_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084669.1"
                     /translation="MLKKINAVLSLFVCITILFFSTNVINGIKTSEALYKNTAEIHMQ
                     YKDDMDFNSYINALYEISTQENINISQYSFTAVNQLTILATNPNANKHWKFSKKNILA
                     RNDSKVHYTNNKNSKYQLKLPNNFLNINIYPFSHVENIGLSEILYVQGNSNKLIPVLK
                     KYGTPTISNINQSDTFQINTNIVLVICYLSIFIVITTIIFAFSKMKEITLKKMLGYNS
                     FDTIKDSIKELNLLFCSLIITFILSIFLSIYYSNENFFLSFLLCSIFHFIISFLFIIV
                     TLSIYHLSSIKNYLKNSRPLKLNLCILNICLFLSMILLLIASTKVINIHNEAENNSLK
                     YWERTTNLYKTNITNQLNRNNTVEENNYLKKASKFVYKIQKNYKTFIIAPYNYATIQE
                     NNKEFFIGQKVYPNFEDYVSQPAGAGICVDLNYLKYYNPISFEESTDLNLINSDPLTT
                     YILVPKKYKEYAKMIEKNYLEDIQFRLEDSPPQAPYKTPNLKNLKIKLIFVNNNQKYF
                     SFNTLYGKAKDNYTITDPIVRVINPEITESLFWGNILTSDGGLFFDQKHTSNQNFFNQ
                     INPYIKEFNLENIINFSYSVFQETGELLSQKKVNKLNAYLQYLLILGLFIIINFQMIF
                     VSFTINSKKIALKKIFGYSPISQVLDLCLLPFTIELFATMCFQLIFSKNTYLYFIIFM
                     IFLINLITSIIIYKKNSFKYFKENIL"
     gene            3513..4133
                     /locus_tag="I0B46_002413"
     CDS             3513..4133
                     /locus_tag="I0B46_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAR2084670.1"
                     /translation="MILSVENFTLSINKKKIKQNWNFSISEKDFIIVTGKSGSGKTTL
                     LNNLSLIDKQYTGNLFYFEKKVSKHDIQRLRRNHISYLFQNYALLENQTVQYNFELAK
                     KFNSNISNDHIYNLLTDFSLPKDILKEKIFLLSGGEQQRIALIRNMLKPFDILFADEP
                     TGNLDKENSTFIMNFLKNLTEKEKKAVVLVTHDLNVLNYGTNIIEL"
     gene            4826..5206
                     /locus_tag="I0B46_002414"
     CDS             4826..5206
                     /locus_tag="I0B46_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAR2084671.1"
                     /translation="MSEQNQNLASNSSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVASG
                     DAQRLSENLKIMQKELQKIWQQLR"
     gene            5188..6102
                     /locus_tag="I0B46_002415"
     CDS             5188..6102
                     /locus_tag="I0B46_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAR2084672.1"
                     /translation="MATIKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKK
                     KVRSNRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTP
                     EQPEIRPARRNHEQHVERGTDHQQELSL"
     gene            6342..7031
                     /locus_tag="I0B46_002416"
     CDS             6342..7031
                     /locus_tag="I0B46_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084673.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFL
                     GCIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYVKDTKV
                     EEACSDLFGDTIPFSTSEQERKERQQSVTYERYEILLQQAINIQNLTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(7151..7237)
                     /locus_tag="I0B46_002417"
     CDS             complement(7151..7237)
                     /locus_tag="I0B46_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAR2084674.1"
                     /translation="MFELFICPLLVGVCISVFSYWLNNRNKK"
     gene            7883..8815
                     /locus_tag="I0B46_002418"
     CDS             7883..8815
                     /locus_tag="I0B46_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAR2084675.1"
                     /translation="MANEIVKYEPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIR
                     FYFSQLKDLSAYKQTAGEVFIKDLARTYDHLMDLRFGRKSNGGLTIERFTMFTDFEIH
                     GEVDNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWA
                     EFSKEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGY
                     RFTWKAEINHADDFSKGKQEDLRIKLFNIEHNGELTQKEKWRAKDRILNLPLGTHEAD
                     FKKQQQTEKEEAEKQSISNELKQDLLENLQNLFD"
     gene            8832..9302
                     /locus_tag="I0B46_002419"
     CDS             8832..9302
                     /locus_tag="I0B46_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAR2084676.1"
                     /translation="MIDKTIKELAEEFHVSKQAIRKRLSADFRENYVQTVTRNGVKTL
                     VVSYKGYLLLKQHFLGSNTTGNQVETVASNDENSIIVFLHEQLQEKDTQIKQMQKLLD
                     QQQQLTLQANQQIEKLQEQLQLAYTEEPEEEELTSVNKENNNAITTEKKWWKFW"
     gene            9386..9703
                     /locus_tag="I0B46_002420"
     CDS             9386..9703
                     /locus_tag="I0B46_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAR2084677.1"
                     /translation="MTISKVIKQKRIEKQLTQEDLAEMLLVSKKTISNWENGRTIPDT
                     ENLIQFAKLFDLSLDNILLGEEKIMVKNYGKKSYKNTNTIISFCNFFKFSFHLNSVRI
                     LLF"
     gene            9993..11174
                     /locus_tag="I0B46_002421"
     CDS             9993..11174
                     /locus_tag="I0B46_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAR2084678.1"
                     /translation="MKNNRIYKNQLNYGLLLPVFLLCIIGLLSLYVALYHDSYTNSKI
                     TKELIKQVLWYSIGFTSAITIYFFNPKILWKLTPIFYGLCIIMLLLLLKFYNPQLAVL
                     TGAKNWFYIAGFSFQPTELVKIGYTLMLAKITVNYQQKSITSNTKDDWIYLLKTFIAT
                     LPIGILLFFQNDLGTMLVFIVIYIFILFTSNINIKIIAPIIAIGVVLSLIFLYLVISD
                     TGRDFLLKIGFHDYQFKRIDSWLNPFLDPNGSSYQLANSLIAIGSGGLLGTGFNVSNI
                     HVPVRESDMIFSVIGENFGFIGSCLVIFLYFYLIYQMYKTCFKSNNLFFTYIGIGIVA
                     MIFFHVFENIGASIGLLPLTGIPLPYISQGGSAILSNFLGLGFMLCCSRYTNEKKGTQ
                     K"
BASE COUNT         4267 a         1694 c         1689 g         3590 t
ORIGIN      
        1 aaattattac aacagtccta acgaagaatt gtttgtaaaa caaacggata aataataaaa
       61 aagactgctc tttcgagcag ccagttaaaa aataatgagt gtttattctt gaaaaagcaa
      121 cgcgcttatg aagcgctacc aaaaatttat catattttat gaaaaataac accccagtaa
      181 aaaatgactg gtaaaaattc acaaagaaac ggaaagaagc aactgcagaa aatcaagcct
      241 taaaaagagc ttttgaccta gaccaatgaa aaatcacaaa gtgagcccct gtatcccttg
      301 gtagagaggc tacttggcat agtcgacatt atgtcaacta tgcaattaaa taactaacta
      361 cttcatatta gcttaataaa ataatcaaaa tcactagggg aaaaaatatt gtataaaaaa
      421 atttttcatt ttttaaataa gaatagtaaa tataaaaata atttaagata tctattacta
      481 ttaatccaga tagagcaaac caagtaaata ttccgtgtgt aaagctaaaa aaattcattt
      541 gaatcaattc aaataaacct aatatagtaa caaatatatt aaaaataata gctgtcaata
      601 ccagtaaatt attttgttta ttttttaaat tatcttcatt taaaatagaa tttatgttag
      661 tactataaaa ttcacaaatt ttttttagtg tctctatatc tggtgttgtt ttgttatttt
      721 cccactttga aatagtttga cgtgtaacat taaattttct tgccacatat tcttgagtat
      781 accctttttt cactcttaat tctcttagct tagatcctaa atcctccatt ttttctccta
      841 tgtaaacgat tatttacata aaattttatc ataaaaatac taacgcgcca aaaaagttct
      901 ttattttata aaacacacta aaataaaata attctagtaa aaaagatagg agaaataagt
      961 tatgaaaaaa tttttatgtc tttctgcttt aatgggtgtc ttattaacta gtggaggaat
     1021 agttagtgct acagaagctt taaatctaga tgtacccgaa gaccataccg ccatttacgg
     1081 aggaggaatg gaagctacag aagaaggatt ttcttctagt aaattgagat atgctgctgg
     1141 cggcggagat ttcaactgtg gggttaatgg atttaaagtt tatgcaaatt actaccatgc
     1201 tagagctaaa catagtgcaa cagctaaaaa tggacgaggc ggtcaagtac gatcagttca
     1261 gaaagctgga gtaagagctt atgcaacatg caatgccaca cttactggaa atactggttg
     1321 gtggaatgtt tattaaaaaa gagagtttaa actctctttt ttatttacat ttgaaaggaa
     1381 attcaaaata tgttaaaaaa aataaacgct gttttatcac tttttgtctg cattacaata
     1441 ctattctttt ctactaatgt tataaatggt ataaagactt ctgaagcgct atataaaaat
     1501 accgctgaaa ttcatatgca atataaagat gatatggact ttaacagtta tataaacgct
     1561 ttgtatgaaa tatctacaca agaaaatatt aatatttctc aatatagttt tactgctgtt
     1621 aaccaactaa ctattcttgc tacaaaccct aacgctaaca aacactggaa attttctaaa
     1681 aaaaatatat tagcaagaaa cgattctaaa gtccattata ctaataacaa aaattctaag
     1741 taccaattaa aacttccaaa taatttttta aatataaata tttatccttt ttctcatgtg
     1801 gaaaatattg gattaagtga aatattatat gtacaaggaa actcaaacaa attaattcct
     1861 gtactgaaaa aatatggaac acctactatt tccaatatca atcaatcaga tacttttcaa
     1921 attaatacta atattgttct tgttatctgc tatcttagta tatttattgt aattactaca
     1981 attatatttg ctttttcaaa aatgaaagaa atcactctaa aaaaaatgtt aggctataac
     2041 tcttttgata ctataaaaga ttctattaaa gagttaaacc tcttattttg ttctttgatt
     2101 ataactttca ttttatctat ttttttgagc atatattaca gtaacgaaaa ttttttccta
     2161 tcatttcttc tatgtagtat ttttcatttt ataattagtt ttttatttat catagttact
     2221 ctaagtatct atcatttatc tagtataaaa aattatctaa aaaattcacg accacttaag
     2281 ttaaatcttt gtatattaaa tatctgtcta ttcttaagca tgatccttct gttaatagct
     2341 tctacaaaag taataaatat ccataatgaa gccgaaaata attctcttaa atattgggaa
     2401 agaactacta atttatataa aacaaatatt actaatcaat taaatagaaa caacaccgta
     2461 gaagaaaata actacttgaa aaaagctagt aaatttgttt ataaaataca gaagaattat
     2521 aaaactttta ttatcgcgcc atacaattac gcaactatcc aagaaaataa taaagaattt
     2581 tttattggac aaaaagttta tccaaatttt gaagactatg ttagtcaacc tgctggtgca
     2641 ggaatatgtg ttgatttaaa ctatctaaaa tattacaatc ctatttcttt tgaagaatct
     2701 actgacttaa atttaataaa ctctgatcca ctaactactt acattctagt acctaaaaaa
     2761 tataaagaat atgcaaaaat gattgaaaaa aattatttag aagacataca gtttagactt
     2821 gaagactctc ctcctcaagc tccttataaa actcctaatt tgaaaaattt aaaaataaaa
     2881 ctaatatttg ttaataataa ccaaaagtat tttagtttta ataccttata tggaaaggca
     2941 aaagataact atacaataac tgatcccata gttagagtta ttaatccaga aataacagaa
     3001 tcattatttt ggggaaatat attgactagt gatggtggat tattttttga tcagaaacat
     3061 acttcaaacc aaaacttttt taatcaaatt aatccgtata taaaagaatt taacctagaa
     3121 aatatcataa atttttctta ttctgttttt caagaaactg gtgaattatt atctcaaaaa
     3181 aaagtaaata aattaaatgc atatctacaa tatcttttaa tattaggatt atttataata
     3241 attaattttc aaatgatttt tgttagtttt actataaatt caaaaaaaat agctttaaaa
     3301 aaaatatttg gatattcacc aataagtcaa gttttagatt tatgtttact cccttttaca
     3361 atagagttat ttgctactat gtgttttcaa ttgatatttt caaaaaacac ttacttgtat
     3421 tttataattt ttatgatctt tttaataaat ctaattactt ctattattat ttataaaaaa
     3481 aatagtttta aatattttaa ggagaatatt ttatgatatt atcagttgag aatttcaccc
     3541 tgtcaattaa taaaaaaaag atcaaacaaa attggaactt ttcaatatca gaaaaagatt
     3601 ttattatagt aacaggaaaa agtggtagtg gaaaaactac tctgttaaat aatttaagcc
     3661 taatagataa acaatatacc ggtaacttat tctattttga aaagaaagtt tctaaacatg
     3721 atatacaacg tctacgaaga aatcatatta gctatttatt tcaaaactat gctcttcttg
     3781 aaaatcaaac agtacaatac aatttcgaat tagcaaaaaa gtttaacagt aatatttcca
     3841 atgatcatat ttataattta ttaacagatt tttctttgcc aaaagatatc ttaaaagaaa
     3901 aaatctttct cttaagcggt ggagaacaac aaagaatagc attaattaga aatatgttaa
     3961 aaccatttga tatattattt gcagatgaac ctacaggaaa tttagataaa gaaaactcaa
     4021 cttttataat gaactttctg aaaaatttaa cagaaaaaga aaaaaaagcc gttgtactag
     4081 taacacatga cttaaatgtt ctaaactatg gaactaatat tatagaatta taatttttac
     4141 ccgtgatgct agctgattta aaactagcac cacgggtatt tattttatat tccatctctc
     4201 ctatcccaaa atttaaataa tcacttaata aaaaggaggc acccaagtga ttttattgaa
     4261 aaaagagtat gaaaaatttt atgatagact tatttattct ttgcaattgc caaaaaaatt
     4321 aaaccgtcaa ttacaaaaga cttatgaaac taaatcaact gaagaatggg agagttattg
     4381 ctagattttt ggtagaagta acaaaaagtg gacatattat cataatgact cgccgaaagt
     4441 ctgataaaaa attggctaaa aatattgctg aatgtttaat aaaactatca gttttagcta
     4501 aatagttagt agtttacaca aaaaagcatt tatgtttttt gttgcaaagc aacaatttca
     4561 tcttcggtga aataagcgaa actttacaaa aagtggctag catttgctag ctttttttgt
     4621 attgtggagc gtggcttttg agggggattc aagggggagc ttgcgcccct ttgacaagcg
     4681 gttatttgca ccaaatacgg aggtatttgg ctagcaaata tagagcttgc caaacccttt
     4741 tagtgagctt tactcattgt acctttttag gtacaatggt attgcgaacg atacgtccgt
     4801 aggacgtact gaaagggtgg gacttatgag cgaacaaaat caaaatttgg ctagcaattc
     4861 ttctaaaaaa tatacctatc gatccgagcc aaaacaaatc agttttcgag tgagcgaatc
     4921 cgaatttgca aagctgaagc aatcggctga agctttgcaa atgagtgtgc cggctttcgt
     4981 gaaagccaag gcacaaaacg cgcgtctggt gacaccgaaa gtggcgccag acattgccca
     5041 ggcaatcgcg cgcgatttag ccaaagctgg agggaatatc aatcaaattg ctaagtggtg
     5101 caatacacac caacatgacg ttgcttctgg tgacgctcaa cgtttatccg aaaacctaaa
     5161 aatcatgcaa aaggagctac aaaagatatg gcaacaatta aggtaagccg aaccacttct
     5221 tgtagtcgtg caattaatta cgcagaacca cgagcaacca ttaaaacagg aataaactgt
     5281 gacgtcgatt atgccaaaag tgaaatgaaa caaattcgca tgctgtatgg aaaagacgac
     5341 cgtgtacagg cacacttatt gattcaatct tttcgaccag gagaaattac cgctgaaaaa
     5401 gctaatcaac taggggcaga gtatgcaaaa aaaatagccc ccgatcacca aatagctatt
     5461 tacacacata cagacaaaga ccatattcat aatcacattg tcattaactc cgttaatctg
     5521 gagactggaa aaaaatttca agctcatggt caagcatttc taaacaaatg ttatgaccta
     5581 aatgacgaga tttgtcttgc acataactta ccaatcactg agcgaggaaa gaaaccagaa
     5641 aaacgaacca tgtctgaaat aaaattaaaa gaaaaaaatg aacctgtttg gaaagacgaa
     5701 attcggattg cgatcgatca aacaatgaaa aacaaaaaaa ccagaaccta tgaccaattt
     5761 tgtaattccc tcaaaacatt ggggattcat tgcttcaatc gcgggaaaaa tttcacttac
     5821 gaattgatcc acaaaaagaa aaaagtacga tccaacagac taggaaagga ctacgaaaag
     5881 gagacgattt tacatgagct ggacagacga gaacgaacct ataaccaaca gcgaaccaac
     5941 gagctacgaa gagctcgagc tagcattcgc gaaactgcag aagaagtacg aagagaacaa
     6001 cgaactctta cgcctgaaca accggaaatt agaccggctc gaagaaacca tgaacaacac
     6061 gttgaaagag gaacagacca ccaacaagaa ttatcgctct aaaacgttaa aagacataga
     6121 aaacgaaata aagaaacaac gtgcttttct cgttccttat acgcaagaaa ggatacaaac
     6181 tacgattgat ttacaagtta aaaaactcca tcaagagcta ttagaccagc gaaataagga
     6241 acagaaaaag gtgcttattc aacgatcatt ggattttctt gcccccttat gttgcttagt
     6301 cctgacactg ttcgcatttt ggttagtatg gggaggctaa aatggcattt tatttaccct
     6361 atctactcat ttttgttagt atttcaggaa gtatttggct catctataaa atttttcaaa
     6421 caagatattc attaaaaggc tctaaaattc gatttaagcg atttttcttt cttggctgta
     6481 tattttcctt gataatcgtt tcaagcggct tattcggcgt tttagaggga aataagcgcg
     6541 tttctagaag tattttatta ggaaatgtca ctcaaaagta tgaatcagcg cgtgataaaa
     6601 agaaaaaaga gcaagcatta gcacaaaaaa tggaagagtt taccacttgt tatgaggaaa
     6661 tgaacgatat ttttgtgaat caagaaaaac gtttgacgga taaaaacatg gaaaaactca
     6721 ctcgtcttta tcaaaacttg ccagaaaaac agcaaaaaga ggttcaagac aactatgagc
     6781 gaaccaaaaa agacgtgcaa tatgtgaaag atacgaaagt ggaagaagct tgtagtgatt
     6841 tatttggaga tacaattcct ttttcaacta gcgaacaaga acgaaaagaa cgacaacaat
     6901 cagtcacata tgaacgctat gaaatcttgc ttcaacaagc aattaacata caaaatctga
     6961 ctaaaaaaga aacagcttta aattatttaa aaagcgtcaa ggaatggtta gatcagcaac
     7021 agcaaaattg aatttgattg aaattcatgt taaactaaac atgcaaatgt tcaaacatta
     7081 gatacacgaa agccccagag aggtcggatt ctctggggct ttttttaggt tgatgtcgcc
     7141 tttactgatt ttacttcttg tttcggttat tcaaccaata actgaataca gagatacaaa
     7201 ctcctacaag aagggggcaa ataaacagtt caaacatcag atacacctcc ttccagtcgc
     7261 aagactgtcg gataggcgac aagttattat accctacaaa attaaatctg tgtggcaatt
     7321 ttaagtatct cttatttccg ggttgttagg gcttgccctg accgcctgta gggcgcttta
     7381 aattccagat aaagtttttt atactaagac tgtcttaaaa cggcttagac aagctcttag
     7441 agcctcaacc ctcctttttg aacaatggtt ttagcaaagg agctggaggc gactgcgcag
     7501 gtataatgtg agagcgtagc gatcacacct ttttggcgac cgtagggagc gaggttttga
     7561 attagcagtt tagaaaagtt cgcgaacctg tttaaagaaa aaataattgt ttttttaggt
     7621 gggaaatttt gtctccctct tttataatct ttttttaaac ctctttttaa acctctttaa
     7681 cgtcggctgt gtccttactc ccccaaggta tacaaaattt tataggcatg aaattatctg
     7741 tttataggca tgaaattatc tgtttatagg catgaaatta tctgtttata ggcatgaaat
     7801 tatctgtttt aggtattgtt tttagacata atatgtccta aaatgatgtc aataaaatca
     7861 tttagactgg agtaacaact aaatggcaaa tgaaatcgta aagtatgaac cagaattaaa
     7921 taccattcct ttaagaaaat ttaccccaat agaaatgaac ttgttttttt ctattgtctc
     7981 tagaatgcgc gataaaggta atcaaaaaat acgcttttat ttcagccaac ttaaagattt
     8041 aagtgcgtat aagcagactg caggagaagt ctttataaaa gatttggcaa gaacatacga
     8101 tcatttgatg gatttacgtt tcggaagaaa aagcaatggt ggattaacaa tagaacgatt
     8161 tacaatgttt actgattttg aaattcatgg agaagtcgat aacccttatg tagatataaa
     8221 agtccacgaa aaagctttgc ctttacttaa taatctagaa agctgggtac gtttctcttt
     8281 aacagaattt cgagatttaa atagcagtta tgctaaaaca atgtttagat tgctaaaaca
     8341 ataccgaact caaggatggg ctgaattttc taaggaagat tttttagagc tattagatat
     8401 tcctaaaagc tatagacaaa caaatataaa tcagtttgta ctcaaaccaa taaaagaaga
     8461 actaactcca ctatttaaag gattaactat tcgtaaaaaa tatggtaaag gtcgaggtaa
     8521 acctgttatt ggttatcgtt ttacttggaa agctgaaatc aaccatgctg acgatttttc
     8581 aaaagggaag caagaagatt tacgtataaa attatttaat attgaacaca atggagaatt
     8641 aacacaaaaa gaaaaatggc gtgctaaaga tcgaatacta aatcttccgt taggtactca
     8701 tgaagctgat tttaaaaaac aacaacaaac cgaaaaagaa gaagcggaaa aacaatcaat
     8761 ctcaaatgag cttaaacaag atttattaga aaacttacaa aacttattcg attgaagcat
     8821 aaggagtatt tatgatagat aaaacgatca aagaactcgc ggaagaattt catgtttcaa
     8881 aacaagcaat taggaaaagg ctatcagctg attttagaga aaactatgtg caaactgtta
     8941 ctaggaacgg agtgaaaacc ttagttgtca gctacaaagg atatttgctt ttaaaacaac
     9001 attttttagg tagtaacacc actggtaacc aagtagaaac cgttgctagt aacgatgaaa
     9061 actcgattat tgttttttta catgaacagc tccaagaaaa agatactcaa ataaaacaaa
     9121 tgcaaaagtt actcgaccag caacaacaac tcaccttgca ggcaaatcaa cagatcgaaa
     9181 aactgcaaga acagttacaa ttagcttata cggaagaacc tgaagaagag gaattaactt
     9241 ctgtcaataa agaaaacaac aatgcaataa caacagaaaa aaagtggtgg aaattttggt
     9301 aacaattacc aactttccat aaagaagaaa tcaatttcta tagactctat ctttctaaga
     9361 ataaaaaata tgtttaggtg atcaaatgac tataagtaaa gtaatcaaac aaaaaagaat
     9421 agaaaaacaa ctaactcaag aagatttagc tgaaatgctt ttagtatcta aaaaaaccat
     9481 ttctaattgg gaaaatggtc gaacaatacc agatacagaa aatttaattc agtttgctaa
     9541 gttatttgat ttatcactag acaatatttt gttaggagaa gaaaaaatta tggtaaaaaa
     9601 ttatggtaaa aaatcataca aaaacactaa tactattatt tcattttgta atttttttaa
     9661 gtttagtttt caccttaatt cagtccggat tcttttattt tagccattca attgccactt
     9721 ggttattaat attttttcta agtttactat taataattaa tacattaata attaatactt
     9781 taattattat ctttaataaa catagaaact tatttcttca cagcatattt ccaattgttt
     9841 tattaatttt tctatacttt atataaaaag atagaactat ttagtaagat aaaaaagtag
     9901 ctaattgtaa ttttcgtcta aaactcaaaa ctagtaaaaa taactttatt gtgttatttt
     9961 tatttataaa ttatgaaaaa ggacctttcg aaatgaaaaa taatagaatc tacaaaaatc
    10021 agttgaatta tgggctttta ttgccagttt ttctgctatg tatcattgga ttactttctt
    10081 tatatgttgc tttatatcac gactcttaca ctaattcaaa aatcacaaaa gaattaatta
    10141 aacaagtatt gtggtatagt attgggttca cttcagcaat aactatttat ttttttaatc
    10201 ctaaaatttt gtggaaatta actcctatat tttacggatt atgcatcata atgttattac
    10261 ttctcttgaa attctataat ccacaattag cagtcttaac tggagcaaaa aattggtttt
    10321 atattgctgg tttttcgttt caacctactg agttagtcaa aataggatac acccttatgc
    10381 tagctaaaat aaccgtcaat tatcagcaaa aatctattac tagtaatacg aaagatgatt
    10441 ggatttattt acttaaaact tttattgcta ctcttccaat aggaatatta ttattttttc
    10501 agaacgatct aggaacaatg ttagtcttca ttgttattta tatttttatt ttgtttacgt
    10561 ctaacataaa tataaaaata attgccccaa taatcgccat tggtgttgtt ttatccttaa
    10621 tatttttata tttggttata tcagatacag gaagagattt cttattaaaa ataggatttc
    10681 acgactatca gtttaaaaga atagactcat ggttgaatcc atttttagat ccaaatggat
    10741 ctagctatca gttagcaaat agtttaattg ctattggctc tggcggactt ttaggtactg
    10801 gttttaatgt atcaaacatt cacgttcctg ttagagaatc cgatatgata ttttctgtaa
    10861 ttggagaaaa ttttggcttt ataggaagtt gtcttgtaat attcctatac ttttatttaa
    10921 tttatcaaat gtataaaaca tgttttaaaa gcaataactt atttttcacc tatatcggta
    10981 taggcattgt tgcaatgatt ttttttcatg tttttgaaaa tataggtgca agtatcggat
    11041 tactacctct aactggtatt ccactcccct atattagtca aggtggttct gccattctta
    11101 gtaatttttt ggggttggga tttatgttgt gttgtagtag atatactaac gaaaaaaaag
    11161 gaacacaaaa atgaattcaa aataacactt tatgaataga atgtctattt caataaaaat
    11221 tatgatacct taaagttaat
//
LOCUS       DACKAC010000086        10787 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.087, whole genome shotgun sequence.
ACCESSION   DACKAC010000086 DACKAC010000000
VERSION     DACKAC010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.087"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(390..1337)
                     /locus_tag="I0B46_002422"
     CDS             complement(390..1337)
                     /locus_tag="I0B46_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HAR2084679.1"
                     /translation="MHLEFSDYESLLVKICEDYYLEDINISEISKKYGITRYKALKYI
                     EDAREKQLVSISINSPYARNYELEEIFKNYFKTNIYILKSSEDLSQHDLLFSKFAADY
                     IQELIKKANVVALSWGDTVYKVIDQFKMGAREELIFTQFIGEIGKYNSLAGSTRLVQK
                     AAERYESSYLTLSIPLYVINETARELMALEPSLANTLATASHADMLISGIGTLSSIDS
                     VEPWNKHRKILFPSMTDTVGFLYGRPFNINGEFIKASEDKTFGLSLKEIFDIPKRIGI
                     CKSKFKAESCLGALNGNFFTDLFLDEKTAWKILSLIKKE"
     gene            1525..2712
                     /locus_tag="I0B46_002423"
     CDS             1525..2712
                     /locus_tag="I0B46_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HAR2084680.1"
                     /translation="MLDEVLEIHKKNVGVLRMETTEAVQDDQDLAKMYTPGVAELSLM
                     IEKNHALARELTISGKLVAVITDGSAVLGLGNVGNQAGLPIVEGKALLYKNLAGVNAI
                     PLAIEQKSVDEIVQTIVNLQNSFAGIHLEDIAAPKCFEIEEKLQEKLSIPVYHDDQEG
                     TAIVVLAGLINAAKIQRKTLTELRVLINGMGASGVATARLLIAAGIKNLTLVDKQGRL
                     KKQDSTLNKYQRELLDHIHDTNNNASVLEDAIVGQDVFIGLSIGNVLTPEMIGKMNDN
                     PIIFALANPVPEITPDLAKESGAKLIATGSSKYPNQVNNILVFPGLFKGLLLSKANKV
                     DLSLQLEVAKQLAAKVAEPTVEKFIPNVFDEGVANVVSQAVLDYLQKLKENEDNIHAE
                     NIK"
     gene            2693..3997
                     /locus_tag="I0B46_002424"
     CDS             2693..3997
                     /locus_tag="I0B46_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAR2084681.1"
                     /translation="MLKTLNRIKIGFIPLSLYIILVLAYLAMTISNIVPENDMLVSLG
                     IMTVFAYLLEEIGKTIPILKDLGGKVLVVTFLPAFLVYKHWLPQSTVTVVSDFMNNNN
                     FLMFFITLLVVGSIASMNRQTLVKATSRIIIILILCDVIGSLIGTGVGILLGLSAWNA
                     YFFIVAPIMAGGVGEGAMPLSIGYAAILSGMGQNDIFGKILPCVMLGSLVAVLLSGML
                     KKIGEKYPQLNGQGNLIDGDDEHLENLKGSAKPVNIEKTLLAGVVAITIYFVAVYVEH
                     LLKSLLGFSIPSPVILLVSLMGAKMLGFIPKNIEEGGNSLYGFTVRGITPPLLFGVGV
                     AKTDWLELIEVFKNPATIVTIIVTVVAIVATAFIAAKFLKMYPVDTAIAVSCCSGQGG
                     TGALAILAAGDRMELMPFAQVAVRLGGAMTVTFAIFLMGLLS"
     gene            4023..5036
                     /gene="citC"
                     /locus_tag="I0B46_002425"
     CDS             4023..5036
                     /gene="citC"
                     /locus_tag="I0B46_002425"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HAR2084682.1"
                     /translation="MFDQTDIQRLFLKNNYIKDQWENLLLDSGIQKKQIEDFQHLDQT
                     LGIYHKEKLVGTVSYQNNVIKYIAVSEKYKDGGHTFNVLVSAIIQAMSQQEIFHYIVF
                     TKPRYQKSFNFLGFETIVSTPKIAVLEMGDSSIKQYLERIPKVNGEKKAAIVMNANPF
                     TEGHYYLVKQAANNNDWVYVFVLEKEQNLFSTKDRLKLVQQGLQKLKNVIVLSGGEYI
                     ISPATFPTYFLREDDEIAKEQMVVDATLFKERIATYLDISARYVGEEPLSPMTRSYND
                     ILETILPPEIKVHVLARKKTEQHQVISASQVRKAYLAGQLEKIKYMVPETTYQYLKNK
                     RER"
     gene            5040..5333
                     /gene="citD"
                     /locus_tag="I0B46_002426"
     CDS             5040..5333
                     /gene="citD"
                     /locus_tag="I0B46_002426"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HAR2084683.1"
                     /translation="MEINKSAIAGTLESSDVQIMISPNTEGIEIDLDSQVIEQFGKQI
                     KKVIYDTLAVYQIKNVKLKVVDKGALDCVIVARLKTAIHRSLGIQHQETPWEV"
     gene            5338..6243
                     /gene="citE"
                     /locus_tag="I0B46_002427"
     CDS             5338..6243
                     /gene="citE"
                     /locus_tag="I0B46_002427"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HAR2084684.1"
                     /translation="MMKERLRRTMMFVPGNNPAMIKDAGIYGADSIMFDLEDAVSMAE
                     KDSARMLVAEALKTVDYGDTELIVRINDLHSEFGRADIFAMVKAGAHVLRLPKTETSE
                     DITEVEKVIIEAENYHNVPIGRTLMMAAIEGASGVLNAPAIAKASDRLIGIAIGAEDY
                     VTNMKTKRYPDGNELFFARSMIVHAARAAGISAFDTVYSDVDDMEGFLNEVRLIHQLG
                     FDGKSCINPRQIPVVNTVYQPSIKEIKRSLDIISAIEEAKEKGSGVISLNGKMIDKPI
                     VERAQRTLMLAKASRLIDEEGNIHA"
     gene            6236..7795
                     /gene="citF"
                     /locus_tag="I0B46_002428"
     CDS             6236..7795
                     /gene="citF"
                     /locus_tag="I0B46_002428"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HAR2084685.1"
                     /translation="MHNKQRRSIPSSVLTKYKKEFQSVSLSPQTIQRCAPKVTTVTNQ
                     TNKLMHSIKEAIEKSGLKDGMTISFHHHLREGDFVFNQVMAVIREMGIKELTLAPSSL
                     TNTMNEMVIECIKDGTVTHITSSGMRGSLGEFISHGGMDNPVILRSHGNRPRAIESGE
                     LKIDVAFLGVPTSDDYGNANATVGKSVFGSLGYAKVDAQYADKVILLTDHLVPYPNTP
                     ISIPQTQVDYVVEVEAIGDPNKIGAGATRFTKNPKELKIAEMVKDVIVHSPYFKDGFS
                     FQTGTGGAALAVTRYLASEMEEKQIKASFALGGITKPTVDLLEKGLVEKVLDVQDFDK
                     GAAEGMARLENQQEIDASFYADPSNKGAVVNQLDVAILSALEIDTNFNVNVMTGSDGV
                     LRGAIGGHQDAATAKLTIISAPLVRGRIPTVVNDVTTLITPGKSIDVLVTEVGVAINP
                     QRKDLIAIFERIPQIPVFTIEELQQKAEKIVGIPEPLQFTDRIVAYVEYRDGSILDVV
                     RQVKEFEEERS"
     gene            7792..8349
                     /gene="citX"
                     /locus_tag="I0B46_002429"
     CDS             7792..8349
                     /gene="citX"
                     /locus_tag="I0B46_002429"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HAR2084686.1"
                     /translation="MMFDTGEIQEIEDILIHKEQRANLQSNLLSKYSNSSLMVFTLNI
                     PGPIKNNPMIEQVFRQGQQSIQQMFLKKKVEMTYTKEINLPSGLDWYVIVTEAPLNLK
                     KWLVTLEEQDALGRLFDLDVLFKKGEQITIVSRQALNHGERPCFICGKSAKVCARNRT
                     HTVKEMQKKIEQIVADSLKGALKHA"
     gene            8342..9205
                     /gene="citG"
                     /locus_tag="I0B46_002430"
     CDS             8342..9205
                     /gene="citG"
                     /locus_tag="I0B46_002430"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HAR2084687.1"
                     /translation="MHDNIQKIAQEAQLAMLYEVTCINKPGLVDPVDPGSHQDMDIFT
                     FLQSSVVLTPYFEEFIQTGLDRQHQPIEETFMAIRQIGLEAETAMFSVTNGINTHKGA
                     IFSLGIFLAICGRLTIWKVPCKKSFFQKEIQKMTKNLLNDFGKIDQTKPKAWTWGEYL
                     FVNHGLTGIRGEAKRGYPCVFDRGLPYYQAYQGSQQDKMIDTLLFLSMEVEDTNLIKR
                     SNNRHVFEEYQLLIRPYFELGGVKTIQGKKYLDYLNQQFKKKNWSIGGSADLLILTIF
                     LDKIMDKGWLC"
     gene            9439..9848
                     /gene="tnpA"
                     /locus_tag="I0B46_002431"
                     /pseudo
     CDS             9439..9848
                     /gene="tnpA"
                     /locus_tag="I0B46_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334252.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(10485..>10787)
                     /locus_tag="I0B46_002432"
     CDS             complement(10485..>10787)
                     /locus_tag="I0B46_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAR2084688.1"
                     /translation="REAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRL
                     KSTNLIERLNQEVRRREKIIRIFPNCVSANRLIGAVLIDQHDEWLSSSRKYIKFAK"
BASE COUNT         3773 a         1675 c         2117 g         3222 t
ORIGIN      
        1 cgtggagaaa taataagaac taccagtcag ataaaacaag ataccgaaga ctcactgcgc
       61 aaagggtggg gaatgaataa taaagttaga tagaaccccc tttttatagc ataaaaaaga
      121 tttagaagga tatatgatcc tattgcatga gaagtacaat aaaattaata agaagtaaca
      181 ttaacgaaaa aacgtggaaa ttattcagtg aatacaaaga aattaaatct tgaaaagtaa
      241 tggagtatgg cttggaaaag ttcaaaaaat atcaagacaa gcttctcaaa aaaagggatt
      301 ttgatatcca ataaaaagag tgaatcattg tactgccctc caaaaaagta gtaccaaaaa
      361 tctaaatttt ttggaggatt ttacatgact cactcttttt ttatcaaaga aagtattttc
      421 caagcagttt tttcatccaa aaataaatct gtaaaaaaat taccattcaa ggcgcctaaa
      481 caactctccg ctttgaactt acttttacaa ataccaattc gttttggaat atcgaaaatt
      541 tcttttaaag aaagaccaaa agtcttatcc tcagatgctt tgatgaattc accattgata
      601 ttgaagggtc ttccgtaaag aaatccaaca gtatccgtca tgcttggaaa taggatttta
      661 cgatgcttgt tccagggttc gacagaatca atagaggata aggtgccaat tccagaaatt
      721 aacatatctg catgcgaagc ggttgctaag gtattagcta aagaaggttc tagagccatt
      781 agttcacgtg cagtttcatt gatgacatat aaaggaatac tgagagtcaa ataagaagac
      841 tcataacgtt cagcagcttt ttgaactaat cgtgtggagc cagctaagga attatatttt
      901 ccgatttcgc caatgaattg agtaaaaatt aattcttcac gagcacccat tttaaactga
      961 tcaatcactt tgtaaacggt atctccccaa gatagtgcaa ccacattggc tttcttaatt
     1021 aattcttgaa tgtaatcggc agcaaatttt gaaaataata aatcgtgttg tgataagtct
     1081 tcactacttt ttaatatata gatattcgtt ttaaagtaat ttttgaagat ttcttctagc
     1141 tcataattcc tagcatacgg agaattaatt gaaatactaa ctaattgttt ttcacgtgca
     1201 tcttcaatat attttaaagc tttatatctg gtgatcccat attttttaga aatttcacta
     1261 atattgatat cctcgagata atagtcttca caaattttta caaggagtga ttcatagtca
     1321 gagaattcca gatgcatagt atagctccta actttttcaa atagatattt tttctcatta
     1381 taccaaaaaa attgaattta gtcttttttt aaatttttaa aaataagcat cattttttta
     1441 aaatttaaaa tgaaagggtt tttttatttt aaaaaaagtg ttaaagtggt tttgtaatta
     1501 aaaaatgtaa aaaggagtga aggtatgctt gatgaagttt tagaaatcca taaaaaaaat
     1561 gtaggtgttt tacggatgga aacaacagaa gccgtgcaag atgatcaaga tttagcaaaa
     1621 atgtacaccc caggtgtagc agaactaagt ttaatgattg agaaaaatca tgctttggca
     1681 agagaattaa caattagtgg taaattagtg gccgttatta cagacgggtc ggcggtatta
     1741 ggtcttggaa atgtaggaaa ccaagctgga ttaccaatcg tcgaagggaa agcgctgctt
     1801 tataaaaatt tagcaggtgt caatgcgatt ccattagcca tcgaacaaaa atcagtagac
     1861 gagattgtac aaacaattgt caatttgcaa aatagttttg cagggatcca tttagaagat
     1921 attgcagcac cgaagtgttt tgagattgaa gaaaaattac aagaaaaact ttctattcct
     1981 gtgtatcatg acgatcaaga aggaacagcg atagttgttt tagctggatt gatcaatgct
     2041 gctaagatcc aaaggaaaac attaactgaa cttcgagttt tgatcaacgg catgggagca
     2101 tctggggttg caacagctag actattaatc gctgctggta tcaaaaatct aacattggtg
     2161 gataaacaag ggagattaaa gaagcaagat agtacattaa ataaatatca aagagagttg
     2221 ttggatcata tacatgatac aaataataat gcaagcgttt tggaagatgc aatcgttgga
     2281 caagatgttt ttatcggtct ttcaatagga aatgttttaa cgcctgaaat gattggaaaa
     2341 atgaatgata atccgattat ttttgcttta gccaatccag taccagagat cacacccgat
     2401 ttagcaaaag aatctggtgc aaagctaatc gctacaggtt cttcgaaata tcctaaccaa
     2461 gtaaacaata ttttagtatt tcccggattg ttcaagggac tattactatc gaaagcgaat
     2521 aaagtcgatc tttcattaca acttgaagta gccaaacaat tagctgctaa ggtcgcagag
     2581 cctacggtag aaaaattcat ccctaatgtt tttgatgaag gagtcgcaaa cgtcgtttca
     2641 caggcagttt tggattattt acaaaaacta aaagaaaacg aggataatat acatgctgaa
     2701 aacattaaat agaattaaaa ttggttttat tcctttatct ctttatatca ttttggtact
     2761 cgcgtatctt gcaatgacga taagcaatat cgttccagaa aatgatatgt tagtttcttt
     2821 gggtattatg actgtttttg cttatttatt agaagaaatt ggtaaaacta ttcctatttt
     2881 aaaagattta ggtggtaaag tattagttgt tacatttttg cctgcatttc tagtctataa
     2941 acattggcta cctcaaagta ctgtaactgt ggtttctgat tttatgaata ataacaattt
     3001 cttgatgttc tttatcacac tactagttgt cggcagtatt gcttcaatga atcggcaaac
     3061 attggttaaa gcaacttcac gtattatcat tattttaatt ttatgcgatg tgattggttc
     3121 tttgatagga acaggtgtgg gaatactcct tgggctatct gcttggaatg cgtatttctt
     3181 tatcgttgcg ccgattatgg ctggtggtgt tggggaagga gcgatgccat tatcgattgg
     3241 ttatgcagca atattatctg gtatgggaca aaatgatatc tttgggaaga ttctaccgtg
     3301 tgtgatgttg ggaagtttag tcgcagttct actgtctggt atgctcaaaa aaattggaga
     3361 gaaataccca caattaaatg gtcagggtaa tttgatcgat ggggatgatg aacatttaga
     3421 gaacttgaag ggttcagcta aaccagtcaa tattgaaaaa acattgttag caggcgtggt
     3481 agcaataacg atttattttg ttgctgtcta tgttgagcac ttattaaaat cactccttgg
     3541 cttttctatt ccttcacctg tgatcttatt agtctctctt atgggggcta aaatgcttgg
     3601 ttttatccct aaaaatatcg aagaaggtgg aaattcgttg tatggattca ctgttcgtgg
     3661 gatcacacct cccttacttt ttggcgtagg ggttgcaaaa acagattggt tagagctgat
     3721 cgaagtattt aaaaatcctg caacgatagt aacaatcatt gtgacggtag tagctattgt
     3781 cgctacagct tttatcgccg caaaattttt aaaaatgtac ccagttgata ctgccatcgc
     3841 agtttcatgt tgtagcggac aaggcggtac aggtgcttta gctatcttag ctgcgggtga
     3901 tcgcatggaa ttaatgccat ttgcacaagt tgctgttcga ctagggggtg ctatgaccgt
     3961 aacgttcgct atctttttaa tgggtctgct atcataagaa cataaagaaa ggacgtgacc
     4021 atgtgtttga tcaaacagat atccaacgat tatttttaaa aaataactat atcaaagacc
     4081 aatgggaaaa tcttttgctt gattcaggaa tacaaaagaa gcaaatcgaa gattttcaac
     4141 atttagatca aacattgggt atataccata aagaaaagtt agtaggaaca gtatcttatc
     4201 aaaacaacgt gattaaatat atcgccgtgt ctgaaaaata taaagatggt ggacatacat
     4261 ttaatgtact agtctctgca ataattcagg caatgagtca acaagagatt tttcattata
     4321 ttgttttcac gaaaccgcgc tatcaaaaaa gttttaactt tttaggtttt gaaacaatcg
     4381 tatctacccc aaaaatagct gtattagaaa tgggagatag ttctataaaa cagtacctag
     4441 agaggattcc taaagtgaat ggtgaaaaaa aagcagcaat tgtcatgaat gcgaacccat
     4501 ttacagaagg gcattattat ttagtaaaac aagctgccaa taacaacgat tgggtatatg
     4561 tatttgtact cgaaaaagaa caaaatcttt tttcgacgaa ggaccgactc aaattagttc
     4621 aacaaggatt acaaaaactg aaaaatgtca ttgttctatc aggtggcgag tatattatta
     4681 gccctgcaac gtttcccacc tattttttac gagaagatga tgaaatagca aaggagcaaa
     4741 tggtcgtaga tgctacttta ttcaaagaaa gaattgccac ttatttagat atctctgcta
     4801 gatatgtggg agaagaacca ctttccccaa tgactcgttc atataacgac atactggaaa
     4861 cgattttacc ccctgaaata aaagttcatg tccttgcaag gaagaaaacg gaacaacacc
     4921 aagttatttc tgcttctcaa gttcgtaaag cgtatcttgc tgggcaattg gaaaaaataa
     4981 aatatatggt tccagaaaca acctatcaat acttaaaaaa taaaagggag cgttaagtaa
     5041 tggaaataaa taaatcagcg attgctggaa cattagagtc aagtgatgtc caaatcatga
     5101 tttcaccgaa tacagaagga atagaaatcg atttagacag tcaagtgatt gagcaatttg
     5161 gaaaacaaat caaaaaagtt atctatgaca cactagcagt ttatcagatt aaaaacgtaa
     5221 aactcaaggt agtagataaa ggggcattag attgcgttat tgttgcacga ttaaaaacag
     5281 cgatccatcg ttccttaggt atccaacacc aagaaacacc gtgggaggtt taattagatg
     5341 atgaaagaac gtttgagaag aacaatgatg tttgttccag gaaataatcc agcgatgatc
     5401 aaagatgcag gtatttatgg tgctgactcg attatgttcg acctggaaga tgcagtgtct
     5461 atggcagaaa aagacagcgc aagaatgctt gtggctgagg cattgaaaac agtggattat
     5521 ggtgacacag aattaattgt tcgaataaat gacctgcact cagaatttgg acgtgcagac
     5581 atttttgcaa tggttaaagc cggcgcgcat gtattacgtt tacctaaaac agaaacctct
     5641 gaagatataa cagaagtaga aaaagtgatt atagaagcag aaaactatca taatgttccg
     5701 atagggcgaa cattgatgat ggcagccatt gaaggtgctt ctggtgtatt gaacgcacct
     5761 gcgatcgcca aagcatctga tcgattgatt ggaatcgcca ttggggcaga agactatgtg
     5821 acaaatatga aaaccaaacg atatccagat ggaaacgaat tgttttttgc tcgcagtatg
     5881 atcgttcatg ctgcacgtgc agcaggcatt tccgcatttg acactgttta ctctgatgtg
     5941 gatgatatgg aaggattttt aaacgaagta cgtttaatcc atcaattagg ttttgatgga
     6001 aaatcttgta tcaatccacg gcaaattcca gtagtcaata ctgtttatca gccgtcaatc
     6061 aaagaaatca aacgatcact agacatcatc tcggcaatcg aagaagcaaa agaaaaaggt
     6121 tctggcgtaa tttctttaaa tggaaaaatg atcgataaac caattgtaga acgtgcacaa
     6181 cggacattga tgttagcaaa agcatcacga ttgattgatg aggagggaaa tatccatgca
     6241 taataaacaa agacgtagca ttccatcaag tgttttaaca aagtataaaa aagaattcca
     6301 aagtgtttca ttatcgccac aaacgattca acgttgtgca ccaaaagtaa cgactgtaac
     6361 taatcagaca aataaattga tgcactcgat caaagaagct atagaaaaat cgggcttaaa
     6421 agatggaatg acgatttcat ttcatcatca tttgagagaa ggtgattttg tttttaacca
     6481 agtgatggcc gtgatccgtg aaatgggcat caaagaattg accttagcac caagttcttt
     6541 aacgaacact atgaatgaga tggttattga atgcatcaag gacggtacag taactcatat
     6601 cacaagcagt gggatgcgcg gcagcttagg tgaatttatt tctcatggag gaatggacaa
     6661 tcctgtgatt ttacgttctc atggtaatcg tccacgtgca attgaaagtg gtgaattgaa
     6721 aatcgatgtt gcatttctag gagtaccaac atctgatgat tatggaaatg ctaatgcgac
     6781 tgttggaaaa tccgtttttg gttcattagg gtatgcaaaa gtagatgcac aatatgcaga
     6841 taaagtcatt cttctaactg atcatttagt tccttaccct aatacgccca ttagtattcc
     6901 gcaaacacaa gtcgactatg tcgtggaagt agaagcaatt ggtgatccga ataaaatcgg
     6961 tgctggtgct acacggttta ctaaaaaccc aaaagaatta aaaattgctg aaatggtcaa
     7021 agatgtcatt gttcactcac cttatttcaa agatggcttt tcttttcaaa caggaacggg
     7081 tggtgcagct ttagcagtaa cgcgttactt agctagtgaa atggaagaaa aacagatcaa
     7141 agcttctttt gcattaggtg gcatcactaa accaacggtt gatttattgg aaaaaggtct
     7201 tgttgaaaaa gtgttagatg tccaagactt tgacaaagga gcagctgaag gaatggcgcg
     7261 gcttgagaac caacaggaga tcgatgcttc cttttatgct gatccaagta ataaaggagc
     7321 ggttgttaac caattagatg tcgctatttt atctgcatta gaaattgaca caaacttcaa
     7381 tgtaaatgtt atgactggtt cagatggtgt gctaagaggt gcgattggtg gacatcaaga
     7441 tgcagcaacg gctaaactta ctatcatctc agcgccgtta gtacgtggcc gaatacctac
     7501 agtagtaaat gatgttacta cattgatcac accagggaaa agtatcgatg tattagtcac
     7561 agaagtaggg gttgctatca atccccaacg taaagatcta atagctattt ttgaaagaat
     7621 tccacaaatt cctgttttta caatagaaga attacagcaa aaagcagaaa aaatagtagg
     7681 catcccggaa cctttgcaat tcacagatcg gatagttgcc tatgtagaat accgagatgg
     7741 ttcaatttta gatgtcgttc gtcaggtgaa agaatttgaa gaagagagat catgatgttt
     7801 gatactggag aaattcaaga aatcgaggat attttgatcc ataaagaaca acgtgctaac
     7861 ttgcaaagca acctgctttc caagtattca aatagttcat tgatggtctt tacattgaat
     7921 attcctgggc cgatcaaaaa taatccaatg attgagcagg tatttcgtca gggacagcag
     7981 tcaattcagc aaatgttttt aaaaaaaaag gtggaaatga cgtacacaaa agaaatcaat
     8041 ttgccaagtg gattggactg gtacgtaata gtaacagaag cacctttgaa tctcaaaaaa
     8101 tggttggtca cgttagaaga gcaagacgca ttaggtagac tttttgattt agatgtcttg
     8161 ttcaaaaaag gagagcagat aacaattgtc agtcgccaag ctcttaatca tggggaacgt
     8221 ccttgtttta tttgtgggaa atcagcaaag gtatgtgcac gtaatcgtac acatactgtg
     8281 aaagaaatgc aaaagaaaat cgaacaaata gtagccgatt ccttaaaagg agcattgaaa
     8341 catgcatgat aatattcaaa aaatagcaca agaagcacaa ttagcgatgc tttatgaagt
     8401 tacatgtatc aataaaccag gattggttga tccagtagac cccgggtctc atcaagatat
     8461 ggacattttt acttttctac aaagtagtgt agttttgact ccctattttg aagaatttat
     8521 ccaaacagga ttggaccgac aacatcaacc aattgaagaa acctttatgg cgattcgaca
     8581 gattggttta gaggctgaaa ctgcgatgtt ctcagtgaca aatggtataa atacacataa
     8641 aggtgctatc ttttcattgg ggatttttct tgctatttgt ggccggttaa ctatttggaa
     8701 agttccttgt aaaaaaagtt tctttcaaaa agaaattcaa aaaatgacga aaaatctact
     8761 taacgatttt gggaagattg atcaaacaaa accaaaagca tggacttggg gagaatatct
     8821 atttgttaac catggattga cagggatacg aggggaagcc aaaagaggtt atccttgtgt
     8881 ttttgaccga ggattgcctt attatcaagc atatcaaggg tcgcaacaag ataagatgat
     8941 agacacttta ctttttctat caatggaagt agaagatacc aatttgatca aacgaagtaa
     9001 taatagacat gtttttgaag agtatcaatt acttattcgt ccttattttg aattaggggg
     9061 tgtaaaaacc atacaaggta aaaaatattt ggattaccta aatcagcaat ttaaaaagaa
     9121 aaattggagt attggtggaa gtgcagactt attgatcctt acaatctttt tagataaaat
     9181 catggacaaa ggatggttat gttgatacta taatacatta ttttgtatta gtaagctaat
     9241 aactaaaaaa tggttcaata caaatatttt ataaaaaatg gtagtgccta tcctttcaga
     9301 ttgtagacaa agaaaaattt ctttgtctac aatttttttg ttaatgactt cagtcagttt
     9361 cgtgcgcaaa gcgtgcgaaa caagaaaacc atgcgtttct acacgtggaa gaactagcat
     9421 atcgaaagga gttttttcat gtcaaatgac gataaaagtt tagcccatac tcgttggaat
     9481 tttaagtatc atattgtatt tacaccaaaa tatcgacgaa aagtaatcta taggcaatta
     9541 cgaaaagata ttggaaaaat acttaggaag ttgtgcaaaa taaaaaacgt ggaaattata
     9601 gaagcacatg caacgccaga tcatattcac atgcttgttc aagtaccatc aaaaataagt
     9661 atatccagtt ttatagttat ctgaaagatc gtagtgctgt gctaatccac gagcagcacg
     9721 taaatcacaa atatagatac ggaaataaat cattttggtc ggaggaatat tatgtgagta
     9781 cagtcgaatt gaatcaaaag acaattgaaa aatacatccg tgaacaagaa gctgatgatc
     9841 gcatttgaga tagtataagt aaaagagagt agatgttgca ataacaaaag ggtaaaaatg
     9901 gactacctcc agtaaaatac cgaaaaatag tttaactttt ggagataagt ccatttaagg
     9961 gtctatagaa tgacaatttt tttaggggat tttactatta ttacttcaag acccgcttgg
    10021 attcgcaaag tgagtgaact cttaaaacta cctgatagta ggttgtttta agagttatct
    10081 gaaagtaaaa attgcattta tatttttgat aaatacgtaa atttatatat acgtatttat
    10141 caaagtttta ggccaaagga atattgtgta aatacactag gattgaatga atagaccaga
    10201 agaaattgcg tccaagaaca aaagaagcat gatagtgctc tatataaatt gggcattcaa
    10261 gaatctgaaa tttaaaggta gaattttagt tagactttta gattttaaac acaaaaataa
    10321 ccatacaagc aacaagaagc aaagtgctat attataaatt gtccgtccgt ttacttacat
    10381 aagatcattg gggctatttt taaaagagaa taatagataa taaatgaaaa aaaagtcaag
    10441 tccacaatcc tgtgtaaaat actatacaat gctctcacta ttttttactt ggcaaattta
    10501 atatattttc ttgatgaaga aagccactca tcgtgttgat caataaggac tgctccaatt
    10561 aaacgattgg ctgaaacgca gtttggaaag atacgaataa ttttttctct tctgcgcact
    10621 tcttgattca gtcgttcaat tagattggta ctctttagtc gattgtggga atttccttgt
    10681 acggtatatt gaaaggcgtc ttcgaatcca tcatccaatg atgcgcaagc ttttgaatat
    10741 tttggttgat cgatataatc atgaatcaat cgatttttag cctcacg
//
LOCUS       DACKAC010000087        10341 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.088, whole genome shotgun sequence.
ACCESSION   DACKAC010000087 DACKAC010000000
VERSION     DACKAC010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.088"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            196..1665
                     /locus_tag="I0B46_002433"
     CDS             196..1665
                     /locus_tag="I0B46_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3991 domain-containing protein"
                     /protein_id="HAR2084689.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVGDDRLDHAVAFLWKDPQTGETVGASYQGTIVDFNRFGKRGTYKHIDKNPTPNHGFN
                     LKIGDPKHLKFFESSIDLLSYAALNREKLQDAWLVSMDGLKHHVISHYVEESISELRR
                     KQTFPQSIEICVDNDRAGHIFYEKEQMKGIVDPFTNKKIRCERGIPNDWQVPKEYKAT
                     YEAVAKEMSVEPEAIMAIHKTETNLQLTNQLVSAHDVQSTFGKMLAKGEPVETIDLKE
                     ACTTVAKELKVCERADGTYNFDRFYSRKANIKDVNAGILLSYKAEQYYKGYKKHEHEF
                     VPEVKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            1676..3685
                     /gene="topB"
                     /locus_tag="I0B46_002434"
     CDS             1676..3685
                     /gene="topB"
                     /locus_tag="I0B46_002434"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAR2084690.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKRSEGFI"
     gene            4031..4627
                     /locus_tag="I0B46_002435"
     CDS             4031..4627
                     /locus_tag="I0B46_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAR2084691.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPEKTLCDIWNPWYNVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            4640..5539
                     /locus_tag="I0B46_002436"
     CDS             4640..5539
                     /locus_tag="I0B46_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAR2084692.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNDIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKERKLAYQRHQQMNREMER"
     gene            5542..5673
                     /gene="ssb"
                     /locus_tag="I0B46_002437"
     CDS             5542..5673
                     /gene="ssb"
                     /locus_tag="I0B46_002437"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAR2084693.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGN"
     gene            5695..6027
                     /gene="ssb"
                     /locus_tag="I0B46_002438"
     CDS             5695..6027
                     /gene="ssb"
                     /locus_tag="I0B46_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAR2084694.1"
                     /translation="MIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEV
                     VADNFQLLESKAATESRAHADQSSTSPSTATFEQRNTATPNNNGLNASQNPFGGQSID
                     ISDDDLPF"
     gene            6072..6305
                     /locus_tag="I0B46_002439"
     CDS             6072..6305
                     /locus_tag="I0B46_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084695.1"
                     /translation="MDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVFVYLVCSYL
                     LKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(6464..6586)
                     /locus_tag="I0B46_002440"
     CDS             complement(6464..6586)
                     /locus_tag="I0B46_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAR2084696.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            6776..7000
                     /locus_tag="I0B46_002441"
     CDS             6776..7000
                     /locus_tag="I0B46_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084697.1"
                     /translation="MIKTQPLLVRFTLRTSHEPINCVVANIEIADKLLILEEGKYTIS
                     VTGHFNKRKQLVIESMQIRNPDHFTRSMSI"
     gene            7443..7649
                     /locus_tag="I0B46_002442"
     CDS             7443..7649
                     /locus_tag="I0B46_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAR2084698.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            7649..7900
                     /locus_tag="I0B46_002443"
     CDS             7649..7900
                     /locus_tag="I0B46_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084699.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEVLLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENLETYENEYWLTKLEN"
     gene            8081..8353
                     /locus_tag="I0B46_002444"
     CDS             8081..8353
                     /locus_tag="I0B46_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084700.1"
                     /translation="MNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQ
                     ALELTYAKFIETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            8798..8977
                     /locus_tag="I0B46_002445"
     CDS             8798..8977
                     /locus_tag="I0B46_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084701.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDVPALKK"
     gene            9036..9392
                     /locus_tag="I0B46_002446"
     CDS             9036..9392
                     /locus_tag="I0B46_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084702.1"
                     /translation="MLKTKKNSYKLKLSILSIICLLGMIISGHLYRVAYTQFIEYKVH
                     SLGLAGDRYVEYVHYSLKCSSYILLWLIFSLFELLNLIAISSYHLDKLNTKSSRYNEV
                     FKENVKLESDLNKLKK"
BASE COUNT         3739 a         1711 c         2119 g         2772 t
ORIGIN      
        1 caaacggcgt taccttcaga agaatttgcg ctaggaaatc cattccgatt ttcacttgaa
       61 acagtgtcag aagtggccaa acgaaaaaat gaaatctatt atgaacaaca attgtcacaa
      121 aaagaacgcg tataaaacaa gtaaaaaaga ctatctgaaa aacggatagt cttttttctt
      181 ttgggaggtt tgaaaatgac cgaaaaaaaa atgagtctta ttgatcgatg taaacagatc
      241 gatatagtgg attttgctcg aaacaatgga atggctgtag tcaataaagg acgagactat
      301 cgcttagaag accatgattc atttgttttt gatcgaagaa aacaacgttt ttattggaac
      361 agtcaaaata ttagtggcga cattattgaa ttagctaaac tttttttcat agacaaagag
      421 atccaagact ctaagcaaca atttaaagca gccacagatt ttattctaaa gaatgaagac
      481 aaaacagaac gagtagaaaa tcttcatttt gagacagaaa agtacaaaga tcaccccgtt
      541 gattatcaac cactcacaga aaaaggaagg aattatctga aggaagaacg aaaactacca
      601 gagtggctga ttgattatgc agaaaaagaa gggttgattg ctgaactaaa gccgaaacat
      661 gaacggcaaa attttttagt tggagacgac cgtttagatc atgcggttgc ttttttatgg
      721 aaagatccac agacaggaga gacagtaggg gcaagttatc aaggaacgat cgtcgatttt
      781 aatcgtttcg gtaaacgggg aacgtataaa cacattgaca agaatcctac gccaaatcac
      841 ggatttaatt taaaaatagg cgatccgaag catttaaaat tctttgaaag tagtattgat
      901 ctgttaagtt acgctgcatt gaatcgggag aaactacaag atgcctggtt agtttcgatg
      961 gacgggttaa aacatcatgt aatcagtcat tacgttgaag agtcgatttc tgaattaaga
     1021 cgaaaacaaa catttcctca atcgattgaa atatgtgtgg acaatgatcg tgcaggacat
     1081 attttttatg aaaaagaaca gatgaaggga atcgttgatc cttttaccaa taagaaaatc
     1141 cgttgtgagc gaggaattcc aaatgattgg caagtaccta aagaatacaa agctacttat
     1201 gaagcagtcg caaaagaaat gagtgttgaa cctgaagcaa ttatggccat acacaaaact
     1261 gaaactaatt tacagctgac aaatcaattg gtgtctgctc atgatgttca atctacgttt
     1321 ggaaagatgt tggcgaaggg cgaaccagta gaaacaattg atttgaaaga agcatgtacc
     1381 acggtcgcga aggaactgaa agtatgtgaa cgcgcagatg gaacatacaa ctttgatcgt
     1441 ttttatagta gaaaagccaa cataaaagac gttaatgcag gaatccttct ttcctataaa
     1501 gcagaacaat actacaaagg ctataaaaag catgaacatg aatttgtacc tgaagtgaaa
     1561 aaggattgga atgatcaatt gaagcatgag atccagcaac aagaaatcag aaagcaaaaa
     1621 cgtgcgatgt tgtttcaaca aggcagacaa caagaaaggg agtgaagtcg tcactatgaa
     1681 aaccgtcatt ttagcagaaa aaccgtcaca agcgaaggct tatgccgata gcttttctaa
     1741 agctacccga aaagatgggt attttgagat ccaagatcga ttgtttacag gagaaactgt
     1801 gatcacgtat ggttttggtc atttagtcga gttagattcg cccgatatgt acgatgaaaa
     1861 atggaaacaa tgggcattag aacatttgcc tattttcccc aatcaatacc actatcatgt
     1921 tcctaaagat aagaaaaagc agtttagtgt cgtcaaacga cagctgcagt cagctgatac
     1981 gattgtgatc gcgactgata gcgatcgtga aggggagctg attgcctggt caatcattca
     2041 acaagcaggc gccaatcaag gaaaaacctt taaaagactt tggatcaact ccttagaaaa
     2101 agaagccatt tatcagggat tccaacagtt acgagacgca gaagagacct atccaaaatt
     2161 tgaagaagta caagcacgtc agatcgccga ttggttaatt gggatgaacg gtagcccact
     2221 gtatagttta ctcttgcagc aaaaaggcat accaggaagt ttttctttag gtagagtcca
     2281 aaccccaaca ctctatatga tttatcaatt gcaggaaaag attagaaact tccaaaaaga
     2341 accatatttt gaaggaaaag cccaagtaat cgctcaaaat ggcgcatttg atgcgaagct
     2401 tgatccaaat gaaacccaag cgacacaaga agcatttgaa gcctatttaa aagaaaaagg
     2461 tgttcaatta ggaaaacagc caggtacgat ccttcaagtc gaaacagaga aaaaaagtgc
     2521 ggctagccca cgtctttttt ctttatcgag ccttcagtct aaaatgaatc aactcatgaa
     2581 agctagtgcc aaagatactt tggaagccat gcaagggttg tatgaaggaa aatatctaag
     2641 ctatccgcga acggataccc cttatattac ggaaggggaa tatgcgtatc tgttggatca
     2701 cttagacgaa tacaagcatt ttttaaaagc cgaagcgatt ccgactccta ttcatacacc
     2761 gaatagtcgg tatgtgaata ataagaaagt ccaggaacat tacgcgatca ttcctacaaa
     2821 aacggttatg acagcagcgg cttttgaaca actaagtcca ctgcagcagg ctatttatga
     2881 acaagtgtta aaaacaacgg tggccatgtt tgcggaaaag tatacctatg aagaaacaac
     2941 gatcttgacg caagttcagc agcttcagtt aaaggcaatc gaaaaggtgc cgctagatct
     3001 aggttggaag aagttatttg gaaaagagag cgacggaaaa gagaaggaag aagagccgct
     3061 acttcctaaa gtgacaaaag gagaaatggt gacggtggat ctacaagttc ttgaaaaaga
     3121 aaccaagcca ccacaacctt ataccgaagg tacgttgatc actgcaatga aaacagctgg
     3181 gaaaaccgtg gatagtgaag aagcgcaatc catattgaaa gaagtggaag gaatcggtac
     3241 agaagcgaca cgggcgaata ttattgaaac cttgaaacaa aaggaatata tcaaggtaga
     3301 gaaaaataag ctcgtggtga cgaataaagg gattttgctt tgccaagccg tggaaaaaga
     3361 gccgctgctt acgagcgccg aaatgaccgc caaatgggaa agctacttgt taaagatcgg
     3421 tgaacgaaaa ggaacacaaa ccacttttct agccaatatc caaaaatttg tctctcactt
     3481 attggaagtc gtaccagggc aaatccaatc cactgatttt ggatcgacgt tacaagaagt
     3541 gaaagcagcc agtgaaaagc aagaagccgc gcgtcattta ggaatttgtc caaaatgcca
     3601 ggaacaagaa gtcttactct atcaaaaggt ggcagcttgt acgtctgaag cctgtgattt
     3661 taaaaggtca gaagggttca tttgaagcaa cgattgtttt aaaagaagac ttcactacag
     3721 gctttgaatt ttctgaaaag aaaaaaacga acttcaagaa aagaacacgc cgcactgcaa
     3781 aataatggag ggttaatctt atgcgtaata ccacacacaa acaacaagaa atcgaaaatc
     3841 aaaaagccaa aattcgttca gaacaggaaa aaatgcgtgc cagcaaacga agactgcagc
     3901 aattgaagaa ccagcagagg attcaaaaag aaatcgaatt agatcgataa aatcttccta
     3961 actattgtat acgtataaac ttatgttata ctgtttttat aagtttatac gtatacaaag
     4021 gagggagaaa atgacaaaag caatggtata caagctgcta gaagaattta atggaacttt
     4081 ggctttgaag gatgccaaaa aagcgggcat ttctcctgta acgatcaaac gtatggtaga
     4141 tcgaggggag ctggatcgag agtatccagg ttttttcacc ttacctggtc aatttcctga
     4201 tgaattgttt atggcgcaaa aaaaatatga acgcggcatt atttcacata ttaccgcgtt
     4261 agatttgtat gatttaacag atatgatccc tagacagatc gatctgactg taccttacgg
     4321 ttatcacgtt tctgaaaaag gattgaagga attcgcagtt gagctacact atacaaaagc
     4381 ggaatggtat gaattaggta agattgaaat aaaaagtcgc tatgggaatc ctattatagc
     4441 ttatgatcca gaaaaaacct tgtgtgacat ttggaatccc tggtataacg ttgaggatga
     4501 aatcaaagta aaagcaatca aaaactacat ggagtcggac agaaaaaatc ttagaaaatt
     4561 gaatgagtat cgacgaattt tgcctacaga taaaacgatg cgctcttata ttatggcttt
     4621 aaattaaagg agaatacaca tgacacctac gcaattaaaa gcaaaagtga agaatatttc
     4681 tagagaaaaa gaagttgatc cgcagcttgt tttaagacat tttatgatgg aaaaattctt
     4741 ggaaaaaatc tcagagtctc cttatcgtga aaattttgtg ttaaaaggcg gcttcttaat
     4801 tggctcaaag tatggcattg aaaatcgaac aactaaagac attgatacga ctttacggga
     4861 aatgaaagta accaaagaaa cattgacaac ggttctaaac gatatttttt caacgccgac
     4921 gaaagaaggc attcaatttg agattcaagg aatgaaagaa acgagggaag ctgattatta
     4981 tccagggttt agcttacgtg tactggcaca tttggaaaac atgcggccag attttaaggt
     5041 agatgttact acaggtgact cgatctatcc tgcgacgatc actcacagtc ataaattgat
     5101 gtttgaggat cgaacgattc ctttggaatc ctatcctaca gaacaaatta ttgctgaaaa
     5161 attaagtgca acctttgatt ttttaacaga taattcgaga ggaaaagatt tttatgatct
     5221 ctatactatt cctaaaatgg agaagatcga tgagaagacc ttgaaagata gtgtgagaaa
     5281 tacatttaca cgtcgaggaa aaactgatcc gttaaagata tactatgaaa aggatatgga
     5341 tgtactgaaa aatagtcacg agctaaaaga tacctggaag aaatatcaaa aaagcaatcc
     5401 ttatgctgcg tcgatcacgt acgatgagac aatggattcg attagtaatt tgatggaaaa
     5461 agtgattcac gtcgaagaaa aagagcgaaa actagcttat caaagacacc aacagatgaa
     5521 tagagaaatg gagagataaa gatgattaat aatgtgacat tagtaggacg cttaaccaaa
     5581 gatcctgatt tacgttatac cgcaagtggt actgcagtag cgacttttac cctagccgtc
     5641 aaccgaaatt tcacgaatca aaacggcaat tgagaagccg attttattaa ctgcgtgatt
     5701 tggcgcaaac cagcagaaac aatggcgaca ttagctaaaa aaggtagctt gattggtgta
     5761 gttgggcgga ttcaaacgcg gacttacgac aaccagcaag gacaacgtgt atatgtgact
     5821 gaagttgtag cagataactt tcaactatta gaaagtaaag cagcgacaga gagccgtgcg
     5881 cacgctgatc aatcgtccac cagtccaagc accgccacct ttgaacaacg gaatacagcg
     5941 acgcctaaca acaatggatt gaacgcttca cagaatccat ttggtggaca atcaatcgat
     6001 attagcgatg atgatctacc gttttagttt gagaaagaag gagtaagcat ggcaaaaaaa
     6061 ttagtctgac gatggatcta ttgatacccg taattgcatt tatagacggg atttctttgg
     6121 gcgtcactga aaatcctttg gtaaatagaa ttgcgaaagg atcactattt cttgtattcg
     6181 tgtatcttgt ctgttcttat cttttaaaaa gagcaggaaa ggataattcc ttttggcgat
     6241 tgactaaagt cacagtagca gcgacagctg ttatacttac ggctattcta ccgctttata
     6301 tgtagagtta acgggaaatc gttgaacttc ttgcgaaata ttgctataat gaacatgaaa
     6361 aagaggagtt gcgctaacaa cccctcatta acagcaccga ttaaagaacg gtggcagaat
     6421 attagttatt tggaaataac cgctgacctg ctaaagtaga cggttatttc tttttgtcat
     6481 ttttaagcat atttacaatc aaactaatca aagcaatggt aaacatacca aaaccaagaa
     6541 tggtttgaat tgtctcatat gcggacaaaa ggcttactcc tttcatagat tttagcactt
     6601 acattcataa gcaccaccac ctttccagaa gtcgccaccg tcttcaaact ttactgtctt
     6661 gactattata gcatatctaa aatcgaataa aaattctcaa ttttagctgt ttttgctata
     6721 attttagcga ggtgatcgtg atggaacaat atattggttt actcgataaa ttagggtgat
     6781 caagacacag ccattgcttg tgcgatttac attacgcacg agtcatgaac caatcaattg
     6841 tgtagtggcc aatatagaaa tagcggataa attgctgatt ttggaagaag gcaagtatac
     6901 tatttcagtg acaggtcact tcaataaacg aaagcaacta gtcattgaat caatgcaaat
     6961 tagaaaccct gatcatttta cgcgatcgat gagcatataa aagaagagct gcagtcctaa
     7021 atgaggattg tagctctttt tgttttacat agaaacagta aaaatgaaaa ggcctgttta
     7081 ggaaggcaca agcgccttct caattgaaag acctttctct cactccaacc gccaccgccg
     7141 ttcgagaaga aaggaagtga acgctacgcg ttcactaaga taaagggtct tgcatagcaa
     7201 gacaaaatag aaaaaatttc ctctcaccaa aaggttcgct cctgttcgtt atctctttat
     7261 tgatttgtca gaccggcaac aagttgcctt ttatcaccaa aaagcgacaa ggacaagggt
     7321 aaatggcacg ctgcgcgccc ttgtcttgtc gcttttcgtt gattcattct ggccaacaaa
     7381 tcaataaaga aagaactaac gaaaggaagt aaaacctttc ctaagaggag gcagaatcaa
     7441 tcatgcgaaa gaaatttgat tattggggtg ttcccttcag cgaattattc ccaaactatc
     7501 atgcaccaca tactgtcgaa tgcgattgtg gagaaagagc gaaatgtatc aaaagctatt
     7561 gtttgtatca atgtccaaca tgcgggaaaa aatatacctt gtcttatggc gattatattt
     7621 taattgatga gaaaggaaag aaacgctgat gaaaacacaa cacaaattat tgactgtaaa
     7681 agaagtcgtt caaattttgg tggacaaaga agtcatttct gaaaaagtag cagaagtttt
     7741 acttattccg cggtactgga cgatagaaac ccctttagta gaggatgcac cgactatgct
     7801 atttgaagac ctagtgaaga ttgcaggata tgaactgcat tatcaagaaa acttggaaac
     7861 ttatgaaaac gaatattggt taaccaaatt agagaactaa aaaggagaaa aaaatatggc
     7921 tcaaaattac tatgacgaat ttgtcaaatt acctttggat aaaatggcac aaaaaatgga
     7981 ggatatgacg ttcctataca acgaaacacg tgttccaaaa aaacattata aagaaaaatt
     8041 gtcggtagct gtggaagaaa tgattgaata aggtgtcgaa atgaatctta ttgcgacgta
     8101 ctatcgaaca ctggaagaac tgaaaaagca aaatgcaaaa tggtttttcc aagccttact
     8161 atgcttggaa gtaggtgtca aaccaagcac tattaaacct tctgaatacc aagctttaga
     8221 actaacgtat gcgaaattta tcgaaacgaa aaaagccaag actgtttcaa gtgaatggtt
     8281 agattatttt gaaaatatca ataagtatgg cgcatattac accatgaaaa aggaagacaa
     8341 cgaaaatgaa taaggtgatt tatcaactaa caacggattc catatcaacg atttgaaaga
     8401 aaaaaaggtc aataatcatt tataccgaaa agtgagaatg tgtgaaacaa ggacaaaaaa
     8461 cgcctagtaa ctaaggaaat ggttcttatt tcgcaccaat cgctccaacc gcccccgccg
     8521 cttaggtggt gcgaaataaa cgggcaaagc ccgataactt aagagaaaga aggtgatcca
     8581 tttgaacaag attattccct tattgttagt actgtttgtt ggcgaaatac ttatgcgaga
     8641 tagtgcagct gtacagttaa acctcgctgt atttttggta atactggtgt tttgtttgat
     8701 cttttggcgg tttgtattag tcctcgtgag tctactattc aagatgattg cagccatatt
     8761 tttcataggg ttatttcgat gaaaggagtg agtaccaatg ccaattgcaa taggaaataa
     8821 acggttaccc gtgacattag atgaaaagag acaaaaagaa ttgcagcaac taaagcagaa
     8881 gtatggcaaa agtgaatcta agattatgtg tattgcgtta gatctattga tcgcgcaaga
     8941 aaaagcagga tttgatgtac cagcgctaaa aaagtgacgt cactttttaa tttgatctat
     9001 ataagtgata tcacttttac caaggaggga attaaatgtt aaaaactaaa aagaattcgt
     9061 acaaattaaa gcttagtata ctatctatta tttgtctttt aggcatgata attagtggtc
     9121 atttgtatag agtggcatat acgcagttta ttgagtacaa ggttcactct ttaggattag
     9181 ctggggatcg atacgttgaa tatgtccatt attcattaaa atgttcttca tatattcttc
     9241 tttggcttat tttttcattg tttgaattgc taaatttaat agcaatatct tcatatcact
     9301 tagataagct aaataccaaa tctagccgtt ataacgaagt gtttaaggaa aatgtgaagt
     9361 tagaaagtga cttaaacaag ctaaaaaaat aaaaagaaga aaacataagt taactaaaat
     9421 tattttatta agaatgatgg aatataagga taaggttacc aacataacga tcattacttc
     9481 tctgatatgc tatggacttc tatcaatcag tattttgtct atagattatg gagtaactat
     9541 actgcaagcc ttagttttgg ctatgcctat gtatctatct ttgtttatca tgcatgaaat
     9601 atataagcta tttgttttat caaaaaatgt agaaatacgg gtaaatattt tggtgaatag
     9661 ttatcaaaaa gaactcaaag aaagaagacg gtggcggtat ttattgaaag gatgaaaaag
     9721 taggatttga catactggta ttcagaaagt gccgtcactt ttaaaacaat ggaaaaggtg
     9781 gagataagat gaagaaaaaa caaactaaac tatcattagc agatatttaa cacagttaac
     9841 cgcaactgaa gatggtgttg ttgaatttga acaatctgaa attgcggtag tagatgatcg
     9901 ttaccttaaa atgccttact tctttgacca agcaaaggtc atttgccttt gtcactacga
     9961 tggggtacgt gattttaggc gttaaaaagt agtgattata tcaactttac gagtaaatat
    10021 aaatttagac atgaaactga attatccaat ggaaaatcat ggtttataat ctagaaaaat
    10081 tatagtaatg attggattat atatattctt tcgtattttc gtacaactgt agcttgtcaa
    10141 aattgagaaa tttaaccgat taggtacttt acatcgttat ttgggtaatt tctaaaacat
    10201 gaagcttacc aacgaataaa ctgagactat cttattaatt gatcaatata agaaaatatc
    10261 tttgaggaga tgatgtacta tgttttcgtc tatccacgat tggttaggtg gtcttttcgg
    10321 cggtggaggt aaaggttttt a
//
LOCUS       DACKAC010000088        10244 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.089, whole genome shotgun sequence.
ACCESSION   DACKAC010000088 DACKAC010000000
VERSION     DACKAC010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10244
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.089"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            162..392
                     /locus_tag="I0B46_002447"
     CDS             162..392
                     /locus_tag="I0B46_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAR2084703.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            489..1790
                     /gene="murA"
                     /locus_tag="I0B46_002448"
     CDS             489..1790
                     /gene="murA"
                     /locus_tag="I0B46_002448"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAR2084704.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1803..1979
                     /locus_tag="I0B46_002449"
     CDS             1803..1979
                     /locus_tag="I0B46_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAR2084705.1"
                     /translation="MSNQRYIILTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2114..2410
                     /gene="yidD"
                     /locus_tag="I0B46_002450"
     CDS             2114..2410
                     /gene="yidD"
                     /locus_tag="I0B46_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAR2084706.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2594..3061
                     /locus_tag="I0B46_002451"
     CDS             2594..3061
                     /locus_tag="I0B46_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAR2084707.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3146..4030
                     /locus_tag="I0B46_002452"
     CDS             3146..4030
                     /locus_tag="I0B46_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAR2084708.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4147..4380
                     /locus_tag="I0B46_002453"
     CDS             4147..4380
                     /locus_tag="I0B46_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAR2084709.1"
                     /translation="MSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4468..4548
                     /locus_tag="I0B46_002454"
     tRNA            4468..4548
                     /locus_tag="I0B46_002454"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4502..4504,aa:Tyr,seq:gta)
     gene            4555..4626
                     /locus_tag="I0B46_002455"
     tRNA            4555..4626
                     /locus_tag="I0B46_002455"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4587..4589,aa:Gln,seq:ttg)
     gene            5141..5848
                     /locus_tag="I0B46_002456"
     CDS             5141..5848
                     /locus_tag="I0B46_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAR2084710.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5908..6552)
                     /locus_tag="I0B46_002457"
     CDS             complement(5908..6552)
                     /locus_tag="I0B46_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAR2084711.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6679..6966
                     /locus_tag="I0B46_002458"
     CDS             6679..6966
                     /locus_tag="I0B46_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084712.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7074..7394
                     /locus_tag="I0B46_002459"
     CDS             7074..7394
                     /locus_tag="I0B46_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAR2084713.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7475..8788
                     /locus_tag="I0B46_002460"
     CDS             7475..8788
                     /locus_tag="I0B46_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAR2084714.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8785..9504
                     /locus_tag="I0B46_002461"
     CDS             8785..9504
                     /locus_tag="I0B46_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAR2084715.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9632..10183
                     /gene="raiA"
                     /locus_tag="I0B46_002462"
     CDS             9632..10183
                     /gene="raiA"
                     /locus_tag="I0B46_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAR2084716.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
BASE COUNT         3416 a         1692 c         2187 g         2949 t
ORIGIN      
        1 ttttgtctca gtcccttttt ttgcatattt ttagcattta tttcctaaaa aatgtaaggg
       61 gattcctata tttttaagta tacttgtttt tccttttata cattttatgg tattatcatt
      121 ttattagtta ttataaaaca atcgttagaa aggacgtcat tatgcaagta tacggaattg
      181 atgcggtgct tcgaatcgtg agtcatttgg cgtttatcta cttagccttc tggtctttac
      241 gttcgttacg cattgaaact tttttcagac caatgcataa tacgcaaatt agaatggcaa
      301 tcgtactttt ttcaattttt ttagggtaca ctgcaagtaa ttttttctta gaagttatcg
      361 ctttatgccg aaatcttttc gttagcctgt aaattttaaa aaaaggtact attttaaaag
      421 caaggatgtt ataatagaaa agattttgag taagtgtatg ctcattggaa ttttttggag
      481 ggaacaacat ggaagagatc atcgtaagag gtggcaatca attaaatgga acagtaagaa
      541 ttgaaggagc taaaaatgct gttttgccta ttttagctgc tagtttgtta gcagaagaag
      601 gtattacgac attagacaac gttccgattc tttctgatgt cttcactatg aatcaagtga
      661 ttcgtcactt aaacgtagat gtagattttg atgaacaaaa gaatcaagta acaatcgatg
      721 cttcaagaca attagaaatc gaagcgcctt atgagtatgt tagtcaaatg cgtgcttcaa
      781 tcgttgtgat gggtccgcta cttgccagaa atggacatgc aaaagtagct atgcctggtg
      841 gatgtgcgat tggtaaacga ccaatcgatt tgcatttaaa aggatttcaa gctttaggag
      901 caaaaattat tcaaaaaaac ggctatatcg aagcaattgc ggatgaattg atcggtaaca
      961 cgatttattt agatttccct agtgtaggtg ctactcaaaa tatcatgatg gctgccgtaa
     1021 aagcaaaagg aacaaccatc attgaaaacg tagctcgcga accagaaatc gttgatttag
     1081 caaacatttt gaataaaatg ggcgctcaag tctatggagc tggtactgaa acaatgcgta
     1141 ttgaaggcgt ggatcattta catgcagtca accactcaat cgtacaagat cgtatcgaag
     1201 caggtacctt tatggttgct gctgcaatga cacaaggcaa tgtgctgatt gcagatgcta
     1261 tctctgaaca taaccgccca ttgatttcta aattgatcga aatgggtgca gaaatcattg
     1321 aagaagaagg cggagtccgt gtcatcggac caaaacatat cttacctaca gatgtaaaaa
     1381 cgatgcctca tcctggtttc cctacagata tgcaagctca aatgacagct atccaattag
     1441 ttgcagaagg tacaagtgta gtgactgaaa cagtgtttga aaatcgtttc caacatttag
     1501 aagagatgcg ccgtatgaac gctcatgtca aaatcgacgg aaatgtagca atcatggatg
     1561 gaaaccatga attgcaaggt gcagaagtat atgcaacaga cttgcgggca gcagcagcat
     1621 tagttttagc tggcttgaaa gcaaatggaa tcacacgtgt gcgtaacttg aattatctag
     1681 accgtggata ttataatttc catatcaaac tacagcaatt aggtgcagac gttgaacgtg
     1741 tagacatgga tcaaacttct gctgaaaaaa cagcacaaac gattgcttaa ggaagtggct
     1801 gaatgagtaa ccaacgatat atcatattaa ctttgataaa gattttagtg gtgatcttat
     1861 tgcttatcct tctttttgtg gccggaacga tgatcggata tggtgtcatc ggtggcggca
     1921 atccatttaa agtattccag ccaagtttgt ggattcatat tcgagacttt tttcattgat
     1981 aaataagaat cacagtggtt ctagtcgata tgaagtgagc catgaagcag aacatgacaa
     2041 aagaaaacag aagggaacgt tgacgttagt cgcgatccct ttttttagta aaagaaaaga
     2101 aggaatctaa ttgatgaaga agatattgat ttatttagtt agaggatatc agcgctttat
     2161 ttcgcctgca tttccaccta gctgccgtta ttacccaacc tgttccaatt atatgataaa
     2221 agcaattcaa acccatggtg cactaaaagg gacggtaatg gggactgcac ggatcttacg
     2281 ctgtcatcct tttgtcaaag gcggtatcga ttatgtacca ttgacattcc aattaacgcg
     2341 aaataagaaa gataaaaaag aaccagatta taaaaaggtt gtgaaaaata cgagtagcac
     2401 tgagcgataa agaaacaatt tggaaaatag cttcttatat tttgcaaaat tgtgagttat
     2461 tgccgaagtg ttggattttg aacaccgtca aaaaaaggtt gtgaaaaata cgggtagcac
     2521 tgagcgataa agaaaaagag gaaaattatt gtcaaagtaa caactccgca actacttata
     2581 tgggggacaa aaaatgatcg aagaactaaa caagaaagat ttaccatggt ccttgttatt
     2641 agatgcagat cctgataaag aaaaagttca agtatatgtt tccagaggga gtggctggat
     2701 atggaaagaa aaagaaaaaa caataggtgt actgatctat gtggcaaggg aaaaagaata
     2761 cgaaatcgtc aatgtagcag tagccccaac gtatcaagga aaggggattg gcggaaaatt
     2821 acttgagaca gcttttcaga agctttcaca actcgcttcc agtcaaactc agatcatcat
     2881 acgaaccggc agtactagca gtgcggcgtt acatctctat caaaaaatgg gctatgtgga
     2941 gattggtcga gtcaaagact attttatcca tcattactct gagcccattt ttgaaaatgg
     3001 agatcaatta agggatcaag taattttagc aattcactta cctaaaaata gctttaacta
     3061 ataaactttc aattcgagaa aatgctttca aatgctaaat agatacatta caatagcttt
     3121 agcaaacaaa aaggagaata tttttatggg aatattagtt tttgattttg gtggatcggc
     3181 tgtcaaatat ggttgttggg atggcaaaga gattaaaggt aagggacaat ttgcaacgcc
     3241 tgaatcttgg gaagaaatga aagcgcaatt atttcaagtt tataaaaaaa tactcgtttc
     3301 ttttgaagtc gaaggaattg gaatcagctc tcctggagta gtgaataatg agaaacaggt
     3361 gattgaggga atcagtgcga ttccttatat tcatggattt aatattttcc gagatttgga
     3421 aactctgttt caactcccag taacgattga aaatgacgcc aactgtgccg gaatggctga
     3481 attttatgaa ggtgctgccc gtgattatca agatgttgct tttgttgtgg caggaactgg
     3541 gattggcggg gcgttgttca cgaaaggaca aatcaataaa ggggcacatc tttatggtgg
     3601 agaatttggg ttgatgttct taaatggcga taagacattc agtaaacttg gtacagcggt
     3661 acagatggct tggcgttatt gtgaacgcaa aggtctcgat aaaaattctt ataccggaaa
     3721 agatgtcttt gaattagcag aaactggaga tgcaatagca aaagaagaag ttgagagttt
     3781 ttatacgtat cttacaaagg gattattcag tattcaattt tcgtttgatc cagaagtgat
     3841 cgtattaggc ggcggtgttt ctgcaaaaga agggctcatt gatgagatca aaagccgaat
     3901 gaagaagctg acagaacaat tcgatctgca tgatttcgaa ccgcagattt tattatgtga
     3961 atacagaaac gatgctaatc ttgtaggtgc agcagcaaac tatctcgctg tacgtcaagg
     4021 ggagaattaa gagctttaga gaaggtgcag cttgcataaa agaagtgaat gaaatgaata
     4081 ggagattttt acctaaggaa tgttttattt gctgcagtat gataaaatat ctgagaggtg
     4141 aaaatgatgt caaaaaaaaa taaagatgtt gaagtgcgta tagaagaagt gaaaaaagat
     4201 atcaaaggta agaattatga agtcactcag ttatttatag ggaaaaaaat gattggtgag
     4261 atcttagcct atgggccaaa agaatatgaa attttctttg gtgaagaaga ttttggaaaa
     4321 gaaaaaagtc ttgaaaatgc gatagaaaca gtgattcgac actggaatct gcacgaataa
     4381 aaaaatatca aaaaaacttt caataaaggg ttgcaaaaat ctggaaaatt aggtattata
     4441 actaagtcag gttgatacaa acctttcgga ggagtagcga agtggctaaa cgcgacggac
     4501 tgtaaatccg ttccttaggg ttcagtggtt cgaatccact ctcctccatt tcattggggt
     4561 atagccaagc ggtaaggcaa gggactttga ctccctcatg cgttggttcg aatccagcta
     4621 ccccagtata ccacaatcgt aagctctcgt tagttcgaga gctctttttt ttgctttgct
     4681 accttgttcc ttgaagattc tgctgattta agatagagtt aagttaacta caagaattgg
     4741 agggatccac atgaaatgga aagaaaaagt agacgaagca gctgaaaagt tatatgattt
     4801 ggtcaaatca gaaaaataca atattgaagt gaatattccg aaaaaaggcg gaaaagcggt
     4861 ccaggtaaaa tcaaaacgcc caacgaatca tacaaaaaaa tggatggcta aaaaccgcta
     4921 ataataacaa aagatccata agtcggcatt ttcatcgatt ttagggtctt ttgttatttt
     4981 ctaggttgaa catatctaaa aaattgtttc tgacaatagg agtaggaaga acgatgtgtg
     5041 tctaaatcct ctgttgatgg atagaaacct ttatcttttt cagttgtgaa tcaggtatat
     5101 accagttata atagaagaaa cgaaaggacg ggtgtacgtt atgtcgaatc aattacctgt
     5161 atatatacag attcatgacc aattaaaaaa tgaaatcgaa caaggtatat ggaaagtcgg
     5221 agaccgtctt ccttcagaaa gagaattagc agtgaaattc aatgtcagcc gtatgacgtt
     5281 gagacaagca attcaaaact tggcagatga aggaatctta gaaagaaaga tcggttcggg
     5341 tacatatgtt gcgcgagaaa aagtacaaga aaaaatgtct ggtgcaacaa gttttacaga
     5401 aattatggaa tcgcaaaatc gagtaccgtc tagtcggaca atctcttatt tcttgacttc
     5461 gccaagctca agtgaaatgg aaaaactaaa cttgagtaaa gatgacacga tcttgcgaat
     5521 ggaacgtatt cgtttcgcag atgatgtccc tatttgtttt gaagttgcca gtgtgccaca
     5581 aaagctgatc gatggctaca gcaaagcaga gattactgaa tcgttgtatc gcacgctaga
     5641 agaaaaaggc gggaaaaaga tcggtccagc gaatcaaacg attactgctg tgttggcttc
     5701 tgagaaaatt gctgactatt tagaggtaaa acgaggagat gcagttcttc gtttgcgcca
     5761 gatttcttat tttgaagacg gtacgccgtt tgagtatgtg cgtacgcaat acgtaggaaa
     5821 tcgttttgag ttttatttgg aaaaataaaa aacaggtgat ccctgattcc ttacttagaa
     5881 ataaaagaga atcagaatca cctgttttta tatatgatca gttaccagtt gctcgtgata
     5941 agaaacagga ccctcttcaa attggacttg tccgttcagc agctcaatga tggattcttt
     6001 aaagtggtca gcactttttt catctacagc acagatgacg ctaacttgat cagtatattg
     6061 gatttctttt ataaaaaaat gttggagttc aagataattc tgtaattttc ctagttgcgg
     6121 atacgtgatc atcacagaga tctcctgttg caaggtacct tctacgattc caatgtcaga
     6181 caatgcttga gagacagcat gagtgtatgc tcgtattaat ccgcctgtgc cgagtttcgt
     6241 tcctccaaaa tatcgagtga ctactgcagc tacattgatc agttgatttt ttttgaggac
     6301 ttccaacatc ggtacgccag ccgttccact tggttctcca tcatcgctgc ttcgctggat
     6361 ttcatttttt tctccaatga tgaaggcgct gcagttatgg ttcgctttcc aatgttcttt
     6421 ttttatttct tgaatgaaac tttttgcttg gtcctctgat tctattcgtc taagagaaca
     6481 gataaagcgt gattttttga tctcaatctc actttgtcca ttttctttta ttgtacgata
     6541 gcgctcgatc atcttgtcac ctcttttttt cattttcttt attcagtata aataaagttc
     6601 taggttgttt caacactgta acggttgcgc aatcgctgta gtatgcgata ataaagacaa
     6661 aggtaggtgg atctggggat gcaggaacga atgaaaaagt acgatgcaat tacgcattat
     6721 ttgaagaaca atggcggctc tcaagtaacg ttgaccttta cccaatttga cgaactgctg
     6781 tttcccagta acggacttcc caaaacagca agggaaagta cagattggtg ggcaaatgat
     6841 tataaacacc ctgaaaaagg tgcctatggc tggataaatg caggctatga agtagttgtc
     6901 atcaatttgg acaaagaata tgtcgtgttc aataaattag tgaaatctag ttggctattt
     6961 gattaaataa accgcgttta gaaaacgttg acaaatttct ttctttttat ataatagagt
     7021 aacagacgcg aagaaaacgt ttgcgtagtt tgttttaaaa aggagagaaa agtatggcta
     7081 aaaaaacaat catgcttgta tgttcggctg gtatgagcac aagtttacta gtaacaaaaa
     7141 tgcaaaaagc tgctgaagca aaaggaatcg actcggatat tttcgctgtg tccgcttctg
     7201 acgcagataa caatcttgca aataaagatg tagatgtact gttattagga cctcaagttc
     7261 gctttatgaa agctgatttt gaaaaacgtc tggaaccaaa aggcattcct ttagacgtca
     7321 tcaatatggc tgattatggt atgatgaatg gcgaaaaagt gttagatcaa gcaatcacgc
     7381 tcataggtga ataaatagaa cagtataact atcgaacaag cgatccaaaa aatagatcgc
     7441 ttgttttttc gtatattttt ttagggcgaa taatatgaat catttttggg gtagaaggct
     7501 tctgacgaga gatattaaag caatagactg tgaggaggta aatttaccaa attatgagaa
     7561 gatcgcagca gtggaacgtt tgggcaactg gattctttgt catcgctgtg gaatgaggac
     7621 gtccgtcatt gatggacagc ttgcagatta cggttatttt tgtacacatt gtctatattt
     7681 agggcgatgc gacactcaac aagatttata tttgtttgat cagcctgaag caaaacctcg
     7741 agaagtcgtc tttttttgga caggacagct tactgaaaaa caaacggaaa tcgcaaagag
     7801 aatactttgc cattcagaaa aaagacatca tcttatttgg gctgtaacag gtgcagggaa
     7861 aacagagatg ctttatccta ttttagtgga aactttaaaa gctggaggaa gagtagcgat
     7921 ttgtacaccg cgagtcgatg tttgtaatga attgttttta cgctatcgtt atgtttttcc
     7981 agaagaaaaa atcacgttgc tccatgggaa tacagcaacg gcttatacct ttacttcttt
     8041 tgttatctgt acgattcatc agctataccg tttttatcgg tcctttgatt taatagtgat
     8101 cgatgagatc gatgcgtttc cttataacgg ggatgcagga ttagctcatg ctgtgcaaac
     8161 agcgattaaa ccagacggac gattcatcta cttaagtgcc acacctgata aaaagttact
     8221 aaaggaaatc aagcagacgt ttgacatcca caaacttgct ttgcgctttc atcggcgttt
     8281 acttcctgaa cccgtcttgg ttttcatgaa taattggcga caaaaatgca agaacaagaa
     8341 gaagataaaa tctttggtgc ggatcattca tcaattaatt aaagaaaacc atatcctttt
     8401 attttgtcca agtatcacga tgatgcatcg tttgaaagcc acacttcaag aggctcttcc
     8461 gagatatgag atcacagatg tttcttcaag agacaatcag cgggcggaaa aagtccagaa
     8521 aatgcgagaa gcaaaatatg acatactgtt aaccacgatg atccttgaac gtggagtcac
     8581 atttgaaaga atatccgtta tcgttttagg agcggatcat ccggttttta ctaaatcttc
     8641 tttggtacaa atcgcaggaa gagccgatcg taaagggcct tatacgaaca gtcaagtata
     8701 ctttttctat gaagaaaaaa ctgcagctat tcgaaaagct tgtcaggaaa tcaaagaaat
     8761 gaatgaagaa gggaagaaga tgctgtgagg tgcagttgct gcagtaaaga tatcgtccaa
     8821 aatttaacat tcagcgagat tattggtgtt tcgaaataca gattacctca gacttgttca
     8881 gtttgtcgcg cacaatttca tttattggaa acaagaggct gttctggctg tcaaaaacca
     8941 atccatttca atctagagag tacgaaaaag aaagtaaagt ctattcccgt ttactgcagt
     9001 gactgtcaag aatggaagaa gaaatatccg acctatgatt ttcagcaccg tgcctttttt
     9061 gtgtacgatg aaaatatgaa ggcctggctc aatcaataca agtttatggg agatatccga
     9121 ctagcaggca cctttgcata taaatggacg cagataaaaa aagaataccc gacatacata
     9181 tattgtccaa tacctttatc tgaaaaaagg cgaacagaaa gaggattcaa tcaagtaagc
     9241 gagatgttga aagcagcaag aattcctttt cagatgctac tcaaaagaga gaagcatctg
     9301 ctggctcaag caaaaaacac gcgaaaggaa agattggaga tgcctcaacc ttttgaactt
     9361 gcagtcacct tttctaagat acaaaataaa aaaatattga ttattgatga tgtctataca
     9421 acaggacaga ctcttttcca cgcagcagat tgtttgcagg tcgcacagcc gaaaatcatc
     9481 cgaaccattt ctttagcaag atgaattaga tgtggaaatt tgatagcgat ttaatttgca
     9541 aataacctag agttcgatat aatagaatta agaagaggga aagagtggtc cttctccttc
     9601 ttgattgtgg tagacgaaag gggtaatttt tatgtttaga tataatgtac gcggcgaaaa
     9661 tatcgaagta actgaagcga ttcgcgacta cgttgagaaa aaagtaggca aactagaacg
     9721 ctatttcagt gacgcacccg atgcaacggc gcatgtgaac ttaaaagttt atacagaaaa
     9781 aacagcaaaa gtggaagtaa cgatcccatt accttatctt gtattacgtg ccgaagaaac
     9841 ttctccagat ttatacgcaa gtatcgattt ggttgttgac aaattggaac gccaaatccg
     9901 caaattcaaa acaaaaatta atcgtaaatc ccgtgaaacg ggcatgaata ctgcagatgc
     9961 tactgcttta ttcaatgaag aggacgaaaa cgaaaatgat tctgaattag atatcgtacg
    10021 tacgaaacgc ctttcattga aaccaatgga tagtgaagaa gcagtcttac aaatgaacat
    10081 gctgggacat aacttcttta tttttgaaga tgcagaaaca aatggtacaa gtatcgttta
    10141 ccgccgtaaa gatggaaaat acggactgat tgaaacagac tgatcagcct tacttaaata
    10201 acaacgaaaa cttgcagcag ctactgacag ctgccgcaag tttt
//
LOCUS       DACKAC010000089         9873 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.090, whole genome shotgun sequence.
ACCESSION   DACKAC010000089 DACKAC010000000
VERSION     DACKAC010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.090"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(22..267)
                     /locus_tag="I0B46_002463"
     CDS             complement(22..267)
                     /locus_tag="I0B46_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084717.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(381..959)
                     /locus_tag="I0B46_002464"
     CDS             complement(381..959)
                     /locus_tag="I0B46_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAR2084718.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(1036..1566)
                     /locus_tag="I0B46_002465"
     CDS             complement(1036..1566)
                     /locus_tag="I0B46_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAR2084719.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSPKLDDLMRKNK"
     gene            complement(1570..2430)
                     /gene="rlmB"
                     /locus_tag="I0B46_002466"
     CDS             complement(1570..2430)
                     /gene="rlmB"
                     /locus_tag="I0B46_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAR2084720.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(2423..2818)
                     /locus_tag="I0B46_002467"
     CDS             complement(2423..2818)
                     /locus_tag="I0B46_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAR2084721.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2815..4227)
                     /locus_tag="I0B46_002468"
     CDS             complement(2815..4227)
                     /locus_tag="I0B46_002468"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAR2084722.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(4224..4760)
                     /gene="cysE"
                     /locus_tag="I0B46_002469"
     CDS             complement(4224..4760)
                     /gene="cysE"
                     /locus_tag="I0B46_002469"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAR2084723.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(5082..6542)
                     /locus_tag="I0B46_002470"
     CDS             complement(5082..6542)
                     /locus_tag="I0B46_002470"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAR2084724.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6712..7869)
                     /locus_tag="I0B46_002471"
     CDS             complement(6712..7869)
                     /locus_tag="I0B46_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAR2084725.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7903..9273)
                     /gene="radA"
                     /locus_tag="I0B46_002472"
     CDS             complement(7903..9273)
                     /gene="radA"
                     /locus_tag="I0B46_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAR2084726.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(9336..9863)
                     /locus_tag="I0B46_002473"
     CDS             complement(9336..9863)
                     /locus_tag="I0B46_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAR2084727.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
BASE COUNT         2508 a         2214 c         1785 g         3366 t
ORIGIN      
        1 aatctcaggc tcgtttgctt tttaaactgc ttcagttaaa aattcgatct caactgtccg
       61 agtaagtaca tctgaatagc tgtaagatac tcgttcaaaa gaattttctt caggatccaa
      121 atcaacaacg aatactgaag gataggtttc tgttaaaacg ccgcggcgtt ctgtttgacg
      181 ctttcttcct gtctgagcaa ccaacatgat tgggctgccg atacgacctt ctaaatcttt
      241 cttgattgaa gctaaagttg ttggcataca gttcacctct actatcaact ataccacata
      301 tttttaaaat tttcaagttt accataaaaa aaaagaaatg caataaaaat aaataagaag
      361 ctcattaaga tttttctaaa ttattcctca ttataaaatt tcaagtgttt ttttattttg
      421 agttttactc tcccgtaagc gctcattact tgcttgtgat ccattgctaa acattcagcc
      481 atgtctacaa aatcaacgcc ttgcatataa taatgaaaaa ttttagcttc tatttttgaa
      541 aactggattt cgcaattttc gattgcttct tttaataaaa gatgctgaat cgctaaaggt
      601 tcttgcaatg tgatctctgc taaaaagtct ggtccttctg tctctatttt ttggtcgatt
      661 gagacttcat ctttttgatc acgacgcttc agcgcgttct gcttcctcaa cagagaatag
      721 atttgattgc ttataatgct tttataataa cttccaaagc taacaccttt gcttttttcg
      781 taagttctca atgcctgata taaagcgatc cttgcttctt gttcccaatc ctcagaaaca
      841 taatttctta aataataggt gtttttcaaa cgtagtaaaa gtggaaaata tttttgatac
      901 agggcagcaa atgcttcttc gtcaccatta cgtgcagcgc gcaataactc ctcagacata
      961 aaataattcc tccataaatt cgtttatgga ggaatcttat catcaatgat tcatcgaact
     1021 gattagaacg atatgttatt tattttttct cattaaatcg tccaattttg gggacaattt
     1081 ttttaactgc tcttcattcc atggagaatt acgtcggaaa tcaataaaat gcatatcttt
     1141 attttcttgg gctattgttt tttccgtttt ttttatcgtc ttatataatt cataagcaga
     1201 agttcgtaat gctccttggg aaaagatcat ccactgttct gctaaatcgc ttgtcgccac
     1261 tgtcacttgt gtcaaacggt cattcagctg accagcgatt ctctcgatat aactgtcagc
     1321 cgtttcgtct tctttcgtaa aaacaacatc caactggtat ttcttataat tttgttggat
     1381 gcccggaact agttgggcat cgaagacaac gatgacttcc attccttcat atttcgcata
     1441 atttgataaa cgatgtaaaa gagcttctct tgcatccgcc attttgtcct gctttttaag
     1501 ctggacaagt tccggccacg aaccaatcat gttgtagccg tctacaatca acagctgttt
     1561 tttcatggtc tactccccct gacgttggga aaaagcctga tacataagca aaccagcagc
     1621 aacactggca tttaagctct ggacgtgccc ggtcatcggg atcgtcagca attcatccat
     1681 ttcttttttc aatcctggac tcattccttt gccttcatta ccaatgacta aagcaattga
     1741 tcctttggca ttccattttt ggaagtttgt gccggtcata tctgtgccaa atacccaaaa
     1801 tcctgtttct ttgagctgtt gcacagcttg taccaaattc gtcacgcgtg caacagggac
     1861 atgttcgact gccccagtgg aagtctttgt tacgataggt gtgattccaa ctgctcggtg
     1921 ttttggtatg atcacaccat cgacgccact tgcgtctgct gtccgtaaaa tcgacccaaa
     1981 attgtgtgga tcttcgatgc tatccaaaat caaaaagaag gggtcttctt ttttctcttt
     2041 tgttcgttca atcagttggt caagggataa atattcataa ggtgtaatcg caagaacgac
     2101 tccttgatgc acgccgtgat cgctcaaagt ttctaatttt tgtttaggaa cccatttgac
     2161 tggcacagct tgttcacgtg cgatttcttt cagttcttct actttttctc ctcgtgaatc
     2221 ttcttgaaga aagagtttat tccctcgccc ttgttgtagc gcttctactg cggcatggtg
     2281 cccaaagacg aaattatctt ctaatgcttc ttcattttga tcagaaactg actgatttcg
     2341 tccagtttgc tttttatttg aatatctatt ttgttttttg tcttcaaatc cacgtttcga
     2401 ttgatgtttg tttgttcttt ctttacccat ttttttcacc aactttctga atacaccagc
     2461 ggatcaattc ttccatccgc tcggtttgtt ttgtcaaatg cagatagccc atgagcgctt
     2521 cgaaccctgt tgcgacccga tacgtcgtaa catcagcatt ttttgcgctc gtatggcttt
     2581 tagcatttct tcctctgcga taataaagtt cttcttcttc tgtcaaaaga tgttcttcca
     2641 gcatttgctg aatcaaaaag gcttgtgcat tcgcagaaac ataattcgtt gccgcacgat
     2701 gcagcttatt gggttttgtt tctccttgat caatcaggtg gtcacgaaca tagatttcgt
     2761 aaatcgcatc tccaacataa gccaaggcta aaccgttcaa ttgtgtgtaa tctctcatgc
     2821 ttctcttctc catcgggttc cttgcggtgt atcttctaat aagatccctt tttctttcaa
     2881 caaatcacga atttcgtcgc ttcggacaaa atcacgattg cttcttgctt gatttcgttc
     2941 ttcgatcaat tgttcaatct cttcatctaa taactcttct gctgtaaata aaataccgaa
     3001 gatcgaaagc cattgcgaga ataaagcttc tccttgtgcc aagacagcgg cagaaacttt
     3061 cggctggtct agataagtgt tcagccactt agcaagctca tagaccactg tgatcccatt
     3121 tgctgcatta aaatcatcgt ccatttcttc gatgaatcgc tgttctaaat tttctaacaa
     3181 agcgatttta tccgcatcat ctgccaaatt ctctgaggca gtttcttggc ggaatttcaa
     3241 attgtcaaac gtattcttca gtcgttgtaa attggtggct gcttctttca tcgtcgtttc
     3301 actgtagcgg atcggacgac ggtattgtgt ggtcgccatg aagaaacgca agacttgtgg
     3361 atcaacgttt ttgatcattt catgtacagt gatgaaattc cctagagatt tactcatttt
     3421 ttcatcgtct tcgccaatcg ttacatagcc attgtgcatc cagtaattag cgaatttttt
     3481 ccctgttttc gcttcacttt gtgcgatttc attttcatga tgaggaaatt ctaaatcttg
     3541 cccgccacca tggatatcga tcgtttctcc taaatgtttt gttgccataa cggaacattc
     3601 aatatgccaa ccgggacgtc ctttgcccca tggtgaatcc caagaaatct cgccaggttt
     3661 tgcttgtttc cataaagcga aatctaatgg gtcttctttt tgctgctgtt cttcccctgt
     3721 acgctgactt gctccgactt ccagttcatc gatggattgg tcacttaatt tcccataatt
     3781 ttcaaattta cgggtacggt aatacacatc acctgaagat tcataagcat agcctttttc
     3841 aaccaacact tgaataaatg ctaaaatatc tggcatatga tccatgactc ttggatgcag
     3901 tgtagcaggc tcgacattca acgctttcgt atcttcttca aatgctcgga tgaaacgatc
     3961 agcaacttct ggtgctgtaa tacctaattc gtttgctgta cggatgattt tatcgtctac
     4021 atccgtgaaa ttcgatacga aatcgacttt atagcctcga tattcaaaat agcgtcgaat
     4081 tgtatcgaaa gcaatggtgc tacgagcatt tccgatatgg atataattat acacagtcgg
     4141 tccgcagaca tacataccga catgtccctt ttgtataggg atgaattctt ctttttttct
     4201 ggtcaatgta ttgtagattt ttatcattct gatctcctca ctttttctcc tttgattcga
     4261 acgacttttg ctggtactcc taccgctgta gcgtcgtctg gaacatcttt caacacaacg
     4321 gcacctgcac cgattttggc gttttttccg atcgttaccg gtcctaatat ctgcgcattc
     4381 gcagagagca tggctccttg tttaacagtt gggtgtcttt tacccgtttc ttttcctgtg
     4441 ccgcctaatg ttacgccatg gaataaaatc acatcgtcct cgatttctgc ggtttcgcca
     4501 atgacgattc ccattccatg gtcgatgaac acgccagttc caatcgttgc tcctggatgg
     4561 atctcaacac cagttaggaa acgccagaat tgagcattga tcttcgccaa taaaaagagc
     4621 cggtgctgat ataaaaaatg agagaaacga tgccaaaaca aggcatgaaa accagggtat
     4681 gtcaaaacga tctcagctgt cgaacgtgca gccggatcgt ttctctttgc tgcatcaatc
     4741 gctcgggtaa accaactcat gactttccac tcctttcgta agaattttat gtaggaaatc
     4801 atcttcttat aaagaaaaga agcgcctttt aaataagttt ccttatcaaa agacgctttc
     4861 cgcgtggttc cacttttctt cataatcaat gattatctct gacagctaac gcctgttata
     4921 cgttcttggc tactttgttt cctataaaac agtttttcct gttttctgtg tttcgccaag
     4981 accactccca gaggcatttc ggcaaccatt cattgatacc ttgcaccacc cggtatctct
     5041 cttgtaatga tcagctgctt acttttttct gttcatcgtg tttagttcag tacttgattt
     5101 aaatgtgcta atgatttttc acgaccaagc aattcgatcg tttcaggcaa ttctggacca
     5161 tgcatttgtc ctgatacagc tacacggatt ggcatgaaaa gattttttcc ttttacgcca
     5221 gtttcttttt gaacagcttt gatggcagct ttgacagttg gagcatctac tacttccatt
     5281 tgttctagct gtgctttgaa tgcgtggaga acagtaggaa ctgtttcacc agcaagtact
     5341 tcttttgctg cttcattcaa tacaggatgt tcattgaaaa atagcgtaga cagatcaacg
     5401 atttctgcag catagctcat ttgaggttga tacaggctaa cgatcttctt cacccattcg
     5461 attgtctttt catctgggtt ttctgctaca cgtccatcag cgattagata aggcagacac
     5521 atgtcagtaa atgcatctag atccattgct ttgatgtaat ggttgtttac ccattgcagt
     5581 tttttcgcat cgaaagctgc gggtgattta cttaggcgtt ttggatcgaa cattttgatc
     5641 aattcttggt gttcgaagat ttcgtcttcg cccacaggtg accagcccaa taatgcaata
     5701 aagttgaaca ttgcatctgg tagatagcct aattcgcgat attgttcgat aaattgcaag
     5761 attgattcgt cacgtttgct tagtttttta cctgtttcac tgttgatgat caatgtcata
     5821 tgtccgaagg ttggtggtgt ccagccaaat gcttcataga tcatcagttg ttttggtgta
     5881 ttggcaatat ggtcatctcc acgtaaaacg tgagtgattt tcatcaaatg atcatcgacc
     5941 gcaacagcaa aattataagt tggcatacca tcgcgttttt gtatgacaaa gtcgccaccg
     6001 atattatcgg attcgaacga aatagcgcct ttcaccatat ctgtaaatgc gtattctgta
     6061 ttgcgaggca cacggaaacg tacaacagat tctagacctt gtgcttcttt ttctgcttgt
     6121 tcttcaggtg tcaagttcgc acatgttcct gcgtaatgag gcatttctcc tcttgcgcgt
     6181 tgtgcttccc gttctgcttc taattcttct tctgtgcaat agcatttgta tgctcgattg
     6241 ctagcaagca gctgatcaat cagcggttga tagatttctt tacgttcaga ttgacggtaa
     6301 gggccgtatt ctccaggatt ttcaggagat tcatcccaat tcattcccaa ccaagcaagg
     6361 ttttccaact ggcttttttc gccatcttcg atattacgtt tttggtcagt atcctcaata
     6421 cgaataataa agtctccatc attgtggcgg gcaaacaaat aattgaataa tgcagttctt
     6481 gcatttccaa tatgcaggtg cccagttgga cttggtgcat agcgtacgcg tactttcgtc
     6541 attttcttcc tcttctttca aactagactt tgactaacag caaaaattcc gtcagttacg
     6601 gcttctcaaa tatcatttat ttaaagccac tcagcacaaa ttttaccttt tctaaggtaa
     6661 atagtaaagc caactggttg aatttcctta ccagttggct gaatttcttt tttattttga
     6721 ttgtttagtc tttttctctg tctctttttt attgtcgatt cctcgtcctg catgtgcagg
     6781 tttagcaaag atcatgcgtc cagcagctgt ttgaagggca cttgttacaa cgacttcgat
     6841 acgttcattc atgtaatgct gtccatcttc tacgacaacc atcgtgccgt catcaaggta
     6901 ggctactcct tgttggcgtt ctgtcccggc ttttactact agaacattca tcgtttcacc
     6961 agggatgacg actggtttta cggcatttgc caacgcattg atattaagaa caggtacatt
     7021 ttggaattca gagactttat tcaagttata gtcattggtt acaatgacac catctaaaag
     7081 cttagccaat ttgatcaatt tgctgtctac ttcactgata tcttcaaagt ctccatcgta
     7141 catttcgaca gatataccct cttctttttg caaggcattt aggatatcta atccgcgacg
     7201 accacgcaca cgtttcaaac tatcccctga gtccgcgatg tattgtagtt cgtaaagaac
     7261 aaaattaggg atcatcaagg ttccttctaa gaatccagtc tttgtaatat cataaatccg
     7321 accgtcaatg atcacgcttg tatccaagat tttatattta tggaaatttt ctcctgattt
     7381 tcgttctaac agttgactct caacttgact agctgtttct tgctcgattt ttttgcttcg
     7441 tgaaccaaaa actttcttcc attcttcgat tcgtgtcgtt cccattctca atcccagata
     7501 gcctaagatg atcatcagta aaatcggcaa tacactattg acgaatggaa tttccatatt
     7561 gtaaagaggg gtcgagatca caacacctaa agtcaatccg atgatcgcac cgatcgcacc
     7621 gaagagaaga taagtcaagc tgacctcact tagtttttgt tctacttgtt tgataatagc
     7681 tgagatatgt ttggccaaca gcaaagataa taaaaagaaa ataagtgcgc ctaacaggct
     7741 gttggtaaat gtattattca gccatccgtt tgactgttga ccgattgcct gccaagctga
     7801 gggtaaaaat gaaataccca agcttgcgcc gacaatgatc atcagcaaag taatgacacg
     7861 tttttgcatc gtttttcctc cgtttcgaca atcatttatt agttagcgga acacacgctt
     7921 caacgtttca gccaaagttg ctactccaac tatctcgatt ccttttggcg cttcccagtt
     7981 acctaagttg tttttaggta ggtatacttt tgtgaagccg agtttttgga cttctttgac
     8041 tcgttgttca atatgactga cacgtctgat ttctccagtc agtccaattt caccgatgaa
     8101 acattccgtt ggctgtgttc ctttttcttt atagcttgaa gcaatactga cagcgatagc
     8161 taaatcaatc gctggttcat ttaattttac cccacctgca gcttttaagt aagcatcttg
     8221 attctgaaga agcaacccag ctcttttttc cagcactgcc atgatcagag atacccgatt
     8281 gaaatcaaga cctgttgttg ttcgtttggc atttccgaac atagtaggtg tgactaatgc
     8341 ttggatctct accaaaattg gacgtgtccc ttccattgcc acaacgatag cagagccagt
     8401 tgcaccgtct aggcgttctt ctaagaaaat ctgtgaagga ttagcgactt cttccaatcc
     8461 atgctcatgc atttcaaaaa tcccaatctc attcgttgag ccaaatcggt tttttactgc
     8521 tcgcaaaatc cgaaaactat gatgttgttc accttcaaaa tataacaccg tatccaccat
     8581 atgttctaac attcttgggc cggcgatcga gccttctttg gtaacatgac cgacgataaa
     8641 aatggctatc ccatttgttt tagcgatttt gagtagttct gcagtggttt ctcttacttg
     8701 gctgacactt cctgccacac ttgtgatgtc tggctgcgtc atcgtttgga tggaatcaat
     8761 gataacataa tcaggggaga tatgttcgat ggcacgactg atttcgttca tatctgtttc
     8821 agcgtataga taaaattctg tgttgatact tccgaggcgt tccgcgcgta attttatctg
     8881 ctctgcgctt tcttcgccgg atacatataa gacttttccg cctatggctg ctaattgctg
     8941 agaaacttgt aacagcaaag tggatttccc gatacctgga tcgccaccaa tcaagaccat
     9001 agaccctggc accacacctc cgcctaacac acggttcagt tcttctaatt ttgttttgat
     9061 ccgaggttct tttttaggga cgacgtctgc gatttttgtt ggttttgctt tttctcctgt
     9121 caaacttgtc cgagttctgc ggtccgttgt atcttgttcg atttcttcga ccatcgtatt
     9181 ccattttccg caatttgggc agcgtcccaa gaacttaggc gacacatacc cgcaattttg
     9241 gcaaacaaat tgtacttttg atttttttgc cattgtaccg cctcctttta tcctaatcat
     9301 gagacattaa atcaagctta agtatcttta acgttttatt tactggaaga accaaatccg
     9361 ccttgacgtt gagcagtcgt ttgatcttga tctgctttta aaaacggcaa aaagatccct
     9421 tggccgatac gctcgccttt tttgatcact acatccttga aaccaaagtt ggtgaattgg
     9481 aacatgatat ggccttcatt tgcctcatta ttgtaataat cactgtcaat cacacctact
     9541 ccgtttgtca acactaaaaa ccgcttcaac ggattgcttg aacggttagc aagctgcaaa
     9601 aattcatctt ctcccatgta agctttgatt ccagtaggca cgagggttgg tttcaattgt
     9661 ttttcatttt ctgatacatc ttttttcaat ccatctgcga ctgtttccaa cccctttttc
     9721 cagcctgctt tccagatact tggaatcacg acctcttctg ctgcttcaaa atcatagcca
     9781 gcagcatgat gtgtagcgcg ctgcggtaga ttgatatttt gatctttata tttagaaata
     9841 acttcaaatc ctctttttct caccggtttt cct
//
LOCUS       DACKAC010000090         9641 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.091, whole genome shotgun sequence.
ACCESSION   DACKAC010000090 DACKAC010000000
VERSION     DACKAC010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9641
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.091"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..416)
                     /locus_tag="I0B46_002474"
     CDS             complement(<1..416)
                     /locus_tag="I0B46_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAR2084728.1"
                     /translation="MTYTHLTPTELVMIEAYFNQSHFVSKVAHLVQRSRQTIYNVYRF
                     LKSGGSAISYYEQYKKNKRNCGRRPIVLPDGQQEYIQKKVAQGWTPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDPHDLPMKGKRKPNGHKEKRG"
     gene            complement(498..848)
                     /locus_tag="I0B46_002475"
     CDS             complement(498..848)
                     /locus_tag="I0B46_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084729.1"
                     /translation="MINNYLHYKAINWNVIEDELDNVVWERATSLFWLDTRVPIENDR
                     SKWANLQLQEQEQLNRLLILLTNIATYQSNELGEIIRDSARSQQEIAIINNFQFTEMV
                     HTKAYNRILSIRQF"
     gene            complement(897..1340)
                     /gene="nrdI"
                     /locus_tag="I0B46_002476"
     CDS             complement(897..1340)
                     /gene="nrdI"
                     /locus_tag="I0B46_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016896697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAR2084730.1"
                     /translation="MQIIKVYYISLSGNTTSFLADLDTYCQKQYQKKVDYVNVKDLVK
                     LGKPLTFDINESYFVFLPTYLEGGNGIETGDVEILTTPLRQVITYNKNRDYCLGIIGS
                     GNRNFNKQFCLTAYQYADDFGFTVLDEFELKGTTEDIERIAETMQ"
     gene            1487..2153
                     /locus_tag="I0B46_002477"
                     /pseudo
     CDS             1487..2153
                     /locus_tag="I0B46_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016896696.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
     gene            2143..2598
                     /locus_tag="I0B46_002478"
     CDS             2143..2598
                     /locus_tag="I0B46_002478"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HAR2084731.1"
                     /translation="MIYKEEDIKIGIVIARFNDFLTKSLLSGCEDGLTRSGVLTENID
                     VAWVPGAYEIPLTAATMAETGKYDAIITLGAVIRGATSHFDVVVNQASSGVSQVGLTH
                     KLPVIFGILTVENIEQAIERSGTKAGNLGFTYATNTIEMINLFKEIKQQ"
     gene            2748..4127
                     /locus_tag="I0B46_002479"
     CDS             2748..4127
                     /locus_tag="I0B46_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HAR2084732.1"
                     /translation="MTIQFKQTLAPTKDNSGKPIILSDATFAQRNEKVLQLMREYDFS
                     SLMIYADKEHGSNFEYLTGFIPRFEEAIQVINQDGTSTLILGNENYNKTSMARTQSEG
                     ILCPFFSLPNQPMDFTKTFADYLKDVKVDASKRIGLVDWKLLSNDMDNFHNHHSIPAF
                     IVDGIHEVFGSDKVVNATQLYMHPKEGARVTNNAEEIARYEYGASLASDAVLEAMNQL
                     TVGQSELAAGNHLNRDGQYPSVVTIAAFGDRFEGANIYPTDNTLNDGDKVALTVAYKG
                     GLSSRSGYAVKEESQLAEHDPGYLEEVVYPYFQAYKWWLDNLTIGRKGGDFYQAFSDF
                     YPQATYGWNLCPGHLVADEEWLSSPFYQDSDATIQSGYIFQLDFIPSQAGHNGVSAES
                     TIAVADAALREQIKAEQPELWARICNRKDYLKEALGLELSEHLLPLASTFAYYRPFLL
                     DKKTAIYFG"
     gene            4264..5106
                     /locus_tag="I0B46_002480"
     CDS             4264..5106
                     /locus_tag="I0B46_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAR2084733.1"
                     /translation="MPLDEQIELLFTIDEGYLNPLKVALTSIRQNNPGQAFRIWLIHE
                     SIAMHTIRELQKLTDYLQFGFEAIKIDGSRWNSAKTEDRYPKEMYFRLLAGEILPKEM
                     KRVIYLDPDILVVNPLLDLWQTDLEGYMLAAATHVGLTDVSTRVNQMRLDVDHAYYNS
                     GVMVIDLDKAREIIKWSDIAQMIEKYNLLLILPDQDILNRLYGKYTKEIPEEIWNYDT
                     RKYMRYFTKSLAQHDIHWVMAHTAILHFCGGPKPWDDKHDNRFTSLYLTYQNQLKRIE
                     DLMK"
     gene            5180..6628
                     /locus_tag="I0B46_002481"
                     /pseudo
     CDS             5180..6628
                     /locus_tag="I0B46_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            6743..8515
                     /locus_tag="I0B46_002482"
     CDS             6743..8515
                     /locus_tag="I0B46_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAR2084734.1"
                     /translation="MNNNETKKTYELRITADEIKSTDPTFTMKNLSLGDNYQPKDAYF
                     RTAEGEITRRNYGKNERKISNQTLPRLAIQMFEQSIVSLPESERATFPVCRYHSGDEI
                     IRGIFPDVDSFKKKRKSIEFLIYKYNEDKQIVIYCWSIFSTLLFVQECLKRFGTSGDE
                     FILVYKGKEKKEIRVGETQTMTNHLLKSYVLHLLKSKNLILRGAPGTGKTFLANEMAA
                     DVVSNGRTTQINELSEDEKSRIGFVQFHPSYDYTDFVEGLRPTTSDDGVVSFTLKDGS
                     FKSFVEKAKKTKKVNGQDNFEEAWAKFFDAVTEASIEGEGYNKLKTLTGKPVRNLLAY
                     ERNEIQGVYPAKTNTYLNHDQIYNVYRGLPGTPKGGFDAYRKAIVKHLKEEFGLKNYT
                     ASTVDSTNNQNYVFIIDEINRGEISKIFGELFFSIDPGYRGHKEGVYTQYANLHENPE
                     EKFYIPNNVYIIGTMNDIDRSVDTFDFAMRRRFTFLEIKAEDSAQNMLTQEKTKELMT
                     RLNEAIVAKEKGGLSTDYQIGASYFLSIDNGEETMEQLWDTKLYPLLKDYFHGEHKAK
                     EKLEILEKVYSNDGDLDEAETSTR"
     gene            8490..>9641
                     /locus_tag="I0B46_002483"
     CDS             8490..>9641
                     /locus_tag="I0B46_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine 5'-monophosphate oxidoreductase"
                     /protein_id="HAR2084735.1"
                     /translation="MKLKPLHDNQEINDSVVQHPIITNLVNRDLSSLEKENFIIFPQQ
                     LNQSKDLSEDNLIFQSRNGKIWTCNVVGILSDSTDELRIHSRFANQMKHEDFFLRYML
                     QRVLNYNVINDHFSASKKMTYYDLLVFLFPYYLNEAMRKGTYKEYVKREYNNANIKGA
                     VDVAKHIRSNVPFVGKVAYRTREFSYNNHLTQLIRHTIEKIQNEYDFLLSGDEDTKEN
                     VVLIKQTTPGYARLNQFNILQENILHPVKHSYYEEYSALQQICIQILSEEKSGFGSDK
                     NQIHGIIIDVSWLWEEYIGKVTGWKHYGRDKGLATMHLFQEPNRSPRYPDFTFNNIPI
                     DTKYKKHLDTRNDYNQLVTYIHIMNLDQADTMKGGFLQPTSDPLPANHGY"
BASE COUNT         3282 a         1601 c         1838 g         2920 t
ORIGIN      
        1 tttcctcttt tttctttatg tccgtttggt tttcttttcc ctttcattgg taaatcgtga
       61 gggtcaaagg ttttatcttt aaacatccga tatagtgtac gtaccgaaca agcaatagga
      121 aactctgctc gaccaataag gacatctgga gtccaccctt gagccacctt tttttgaatg
      181 tattcttgtt gcccatctgg taacacaatc ggacgtcttc cgcagttacg tttgtttttc
      241 ttatattgtt catagtagct tattgcgctt ccaccagatt taagaaaacg atagacatta
      301 taaatggttt gtcgagaacg ttgtactaga tgggctactt ttgaaacaaa gtgtgattga
      361 ttgaaataag cttctatcat cacgagttca gttggtgtaa gatgggtata ggtcatttgt
      421 actcactcct tataattctt tcgacggaac tattttgagt gtagcacaaa tgactttttt
      481 agttgtctag cttaatttta gaattggcgt atgcttaaaa tgcggttata ggcttttgta
      541 tgaaccattt cagtaaattg aaaattattt atgattgcaa tctcttgttg ggaacgtgca
      601 ctatctcgaa taatttctcc taattcattg gattgatagg tagcgatatt agtcaaaaga
      661 atcaacaagc gattgagctg ctcttgttct tgtaattgta aatttgccca ttttgatcga
      721 tcattttcga tggggacacg tgtatccaac caaaaaagtg aggttgctct ttcccataca
      781 acgttgtcta attcatcctc aatgacattc cagttaattg ctttatagtg taaataatta
      841 tttatcatag tacctcctaa aatattgttt gaaaggggat gatatggtgt cattttttat
      901 tgcattgttt ctgcaattct ctctatatct tctgtcgtac cttttagttc aaattcatcc
      961 aacacggtaa aaccaaaatc atcggcgtat tgataggctg ttaggcaaaa ttgtttattg
     1021 aagtttctgt tgccactacc aataattcct aaacaatagt ctcggttttt attgtaagta
     1081 atcacttggc gcaatggtgt tgtcaaaatt tcgacatctc ctgtttcaat accatttcca
     1141 ccttcaagat aagtaggcag aaatacaaaa taggattcat tgatgtcaaa agtgagtggt
     1201 tttcctaatt tgactaaatc ttttacattc acataatcta ccttcttttg atattgtttt
     1261 tgacaatacg tatctaaatc tgcgagaaaa ctcgtggtat ttccacttaa actaatataa
     1321 tagactttaa ttatttgcat gtatcacacc tctttttaat aaataaccac atatatagtg
     1381 cttgttttaa ttagaaacgc tatatatagt gtttattgta catgacatat cgttatagaa
     1441 aattgacgat ggtcaattat tttgatttta gaggagagaa aattacatga aaaattcagg
     1501 ttgtttatgc accgttccac tcttcgcctc tctttcatta gatgaaaaaa atgagataaa
     1561 gtgtttgctt caccatcaga cgtttaaaaa aggagaaatt attttttcac caagtagtga
     1621 agaacaactg ttgattatat ctttaggggc tatgaaaata tatcgaatta gtagaactgg
     1681 gaaggagcaa ttaattcgta tcgtaaaaga aggggattac gacggtgaga actatctctt
     1741 tggtctaccc aatgactgcc tatatggtga agctgttgaa gaaaccaaaa cttgcactct
     1801 ctataaatcc gactttaata aagtgatggc acgctatcca cagctcagtt atcgattgtt
     1861 acaattaaat gcttgcaaaa cagtagctgt ggaaaaaaca gttgcagctt ctagcgtttg
     1921 atcgtataga ggaccggtta gccttatatc ttaaccaact atctatagca gcaaaaatgg
     1981 gggagcctat tactttacct atctctatga gagagttggc aaactatcta gctaccagtc
     2041 ctgaaactat ttctcgtaaa ttaagagatt ttcaagaaag acaattgatt actagaaaag
     2101 gaaagcagat tacactatta gaagaatttt ggtttatatt taattatata taaagaagaa
     2161 gatattaaaa ttgggattgt cattgcacga ttcaatgatt ttttaactaa aagcttacta
     2221 agtggttgtg aagatggcct tacgcgttca ggcgttttga cagaaaatat cgatgttgct
     2281 tgggtgccgg gggcctatga aatcccactt acagccgcaa cgatggctga aacaggtaaa
     2341 tatgatgcca ttattaccct aggtgcggta atcagaggag ctacaagtca ctttgatgtg
     2401 gtggttaacc aagcgtcaag tggggtatct caagtaggct taacccataa attaccggtc
     2461 atctttggta tcttaaccgt tgaaaacatc gaacaagcca ttgaacgctc tggtactaaa
     2521 gctggtaacc ttgggtttac ttatgcaacg aatactattg aaatgattaa tttattcaaa
     2581 gaaattaaac aacaataagt catcattcta tatgaaaata tctgtaacag catagggcta
     2641 gctttaattt tcaatcatgg taagatggaa gataggaaaa taaagcgcat tcaattaatt
     2701 atcaagcctt tctgatgatt tgcgcaaaaa gcttaaggag acttcatatg acgattcaat
     2761 ttaaacaaac attagcgcca accaaggata attctggaaa accaatcatt ttaagtgacg
     2821 ctaccttcgc tcaacgtaat gagaaagtcc ttcagttaat gcgtgaatat gacttctcat
     2881 cattgatgat ttatgcagat aaagaacacg gtagtaactt tgaatattta actggtttta
     2941 ttccacggtt tgaagaagct atccaagtaa tcaaccaaga cggaacgtcg acactgattt
     3001 taggtaatga gaattacaat aaaaccagta tggcacgtac acaaagtgaa ggtattcttt
     3061 gcccattctt ctctttacca aaccaaccta tggatttcac taaaactttt gctgactatc
     3121 ttaaagacgt taaagtagat gcttcaaaac gaattggttt agtagactgg aagcttttat
     3181 ctaatgatat ggacaacttc cacaaccatc attcaatacc agcatttatt gtagatggta
     3241 tacatgaagt tttcggttct gacaaggtag tgaatgcaac ccaactatac atgcatccaa
     3301 aagaaggtgc tcgtgtgaca aacaacgcag aggaaatcgc tcgttatgaa tatggtgctt
     3361 cccttgcttc tgatgctgtt cttgaagcaa tgaaccagct aaccgttggc caaagtgagt
     3421 tagcagcagg taatcactta aatcgcgatg gtcaatatcc atctgttgtg accattgcag
     3481 cctttggtga tcgctttgag ggtgctaata tttatccaac tgataatacg ctaaatgatg
     3541 gcgataaagt tgcattaact gtagcctata aaggaggctt gtctagccgc tctggctatg
     3601 cagtaaaaga ggaaagtcag ctcgctgaac atgatccagg atacttagaa gaagtggttt
     3661 acccatattt ccaagcctat aaatggtggc tagataattt aaccattggg agaaagggtg
     3721 gcgatttcta ccaagcattc agtgacttct atccgcaagc tacttatggc tggaatttgt
     3781 gcccagggca tttagtagct gacgaagaat ggttaagctc accattttat caagactctg
     3841 atgcaacaat tcaaagtggc tacatcttcc aactagactt tattccttca caagcaggcc
     3901 acaatggtgt ttcagctgaa tcaacgattg cggttgcgga cgcggcacta cgtgaacaga
     3961 ttaaggctga acaacctgaa ttatgggcac gtatctgtaa ccgaaaggat tatctaaaag
     4021 aagcattagg tttagaatta tcagaacatt tactaccctt agcgagtacc tttgcctact
     4081 atagaccttt cttattagac aaaaaaactg ctatctactt tggataaaaa aatgcccctc
     4141 aagattgttt gccaaacttg agggtttttt gtctattttt aagtgaataa tgacggacta
     4201 tccaaacgga atcgccataa agatgttaat atatgtataa tattataaag aaattggagg
     4261 acaatgccct tggacgagca gattgaatta ttatttacca tagatgaagg ctatttgaat
     4321 cccttaaaag tggctttaac atcgattcgt cagaataatc caggccaagc tttccgcatt
     4381 tggttgattc acgaatccat tgcaatgcat acgattcgcg aattgcagaa gctaacggat
     4441 tatctacagt ttggttttga agcgattaaa attgatggta gtcgatggaa tagcgctaaa
     4501 actgaagata gatacccaaa agagatgtat ttccgcctgc ttgcgggtga aatattacct
     4561 aaagaaatga aacgcgtgat ctatttagac ccagatattt tagtggttaa ccctttatta
     4621 gatttgtggc aaacagattt agaaggttat atgctagcag ctgctacaca tgttggattg
     4681 acggacgttt ctacacgagt aaaccaaatg cgacttgacg ttgaccatgc ttattacaat
     4741 tctggtgtga tggtgattga tttagataaa gctagagaaa ttattaaatg gtcagatatt
     4801 gcacaaatga ttgaaaaata taacttgcta ttaatcctac ccgaccagga tatcctcaac
     4861 cgattatatg gtaaatacac taaggaaatt ccagaagaaa tttggaatta tgatacgcgt
     4921 aaatatatgc ggtatttcac caaaagtctc gctcaacatg atattcactg ggtgatggcg
     4981 catactgcta ttctccattt ctgtggtggc ccaaaaccat gggacgataa acatgataat
     5041 cgttttacca gtctttatct tacctatcaa aatcaattaa agcgtattga agatttgatg
     5101 aagtaaagtg aaacaagtgt ttttcattac ctacggtggt ttaatgctta tttattatcg
     5161 ttaaatcatg cttatacact ttacaaaatt tgattttcga aatggccgtc caacgaagtc
     5221 tgcgatctta accacgagtt ccattgacat ggccaaaaag tattatcgag cgatcaaaga
     5281 aatgacgaaa gaacctgact ggttaacaaa agagtttcct aatcagccca ttcgtgaagg
     5341 aagaacgatg gaggatcctg attttccacg aatcgctatt acctattcat tagaagaaaa
     5401 taaccaagat tcaagtgccc aacaagaaga aatgaaaaaa attattgagg aatataatca
     5461 gtattttgat actgcctggt cattagcaga cattgagcgt tataacggag acatcaataa
     5521 ttgtttggca cgaaaaagag cggaattcaa acaatttggg aaacaaatcg atttagtaat
     5581 cgtagtggat cgtctattga caggttttga cgcacctacg attcaaaccc tatttgtgga
     5641 tcgtaatcta gagtatgcag gattgattca agctttttca cgtacgaacc gtacgtatcc
     5701 tgaaaagaca aaaggattga ttgtgacgtt tcgtaagcca gcgacaatgg agaagaatgt
     5761 cgaggatgcg acaaaactgt attcagaagc aaaagaagaa tcgggtttga tttatccaag
     5821 ttatcaggaa tccaaaaaaa gattcaagca agcgtatggt aagttaaagg aggtttcatc
     5881 catgaaatcc attgatgaac atacgccact tgaaatccgt atcgagtacg ttaaagcatt
     5941 tcaagaattg aatcgtgcgt atgaatcgtt agtgacttat gacgattaca atgatgacat
     6001 ggtgcagtct tcaactttga acaatcaaat tcaacttttg gaagaacaaa ttggcgtgta
     6061 cgagaccgtg aaggggtcat taatagagga agaaccagaa aaagagggag aagatttttc
     6121 aacgatcgaa ttttatagtg aaaataccac taagttgtat gatattgatt ctacttatat
     6181 tgatcaatta ctaggaactt atgctgcgaa tagttcagat gcacgagatg aaattgaaaa
     6241 agctttggct aaattgaata aaacagaagg agtcaaagaa gtttaccgac aaattttaaa
     6301 tgcatttgat gtggggactt tagatagcaa tgaagatatt tttgtcatta aacgtcgcta
     6361 ttttacacaa gcaagtgatg aattgattca tgccttttcc aatgagtggt ttgtttctga
     6421 gaaagaatta catgcttcta atctgcaata tatgccaggt gaagatccaa ttcctaatat
     6481 gaaagcaatt atcaacagta aggattacga aggatataaa gccaaacatc caaaagcgaa
     6541 gccatttaaa tacccacaag aaatgaagcg agcctggcgt aaaatgttag atgatgagtt
     6601 gattccactt gagaatgaat tgaggtaaac gagaaaattg aagaaattgt atgataaaca
     6661 tgttaataaa tgatgctcga aataaaattt ggttttattt ttacattgat acggtaaata
     6721 aaatttgaaa gtagaagaaa atatgaacaa caatgaaaca aaaaaaacat atgaactaag
     6781 aataactgct gatgagatta aatcaacaga cccaacattt actatgaaga atctaagctt
     6841 aggggataat tatcaaccaa aagatgctta cttccgtacg gcagaaggcg aaataactag
     6901 aagaaactac ggtaaaaatg aacggaaaat ttcaaatcaa acattaccgc gattagctat
     6961 tcaaatgttt gaacaatcga ttgtttctct accagaaagc gaaagagcaa catttcctgt
     7021 gtgtcgatat cactcaggag atgagataat acgagggatt tttccagatg ttgacagttt
     7081 caaaaaaaaa aggaagtcca ttgaattttt gatttataaa tataatgaag acaagcagat
     7141 agtcatctat tgttggagta ttttttcaac tttattattt gtacaagaat gtttgaaacg
     7201 ttttggaaca tctggggatg aatttattct tgtttataaa gggaaagaga aaaaggaaat
     7261 acgagtagga gagactcaga ctatgaccaa tcatttgctg aaatcatatg ttttacattt
     7321 actcaaatct aaaaacctta ttctacgtgg cgctcctgga acagggaaaa cattccttgc
     7381 aaatgaaatg gcagctgatg tcgtttcaaa tggcagaact acacaaatca atgaattgag
     7441 cgaagatgaa aagagccgaa ttggatttgt ccagtttcat cctagctatg attatactga
     7501 ttttgtcgaa ggtttacgtc caactacatc cgatgatggc gttgttagtt tcaccttgaa
     7561 agatggaagc tttaagagct ttgttgaaaa agcaaagaaa acaaagaaag taaatggaca
     7621 agataatttt gaagaagctt gggctaaatt tttcgatgcg gtaacggaag ctagtataga
     7681 gggagaaggc tacaataaac ttaaaacatt gacaggtaaa ccagtacgca atcttctagc
     7741 ctatgaaaga aatgaaattc aaggtgttta tcctgctaaa accaatactt atctaaacca
     7801 tgaccaaatt tataatgtct atcgtggatt gccaggtacg ccaaaaggtg gatttgatgc
     7861 ttatcgaaaa gcgattgtaa aacatttaaa agaagaattt ggcttaaaaa actacacggc
     7921 gtcaacggta gattctacga ataaccaaaa ctatgtgttt atcattgatg aaattaatcg
     7981 gggcgaaatt tccaaaattt ttggcgaatt atttttctca attgatccag gatatcgtgg
     8041 gcataaagaa ggtgtttaca ctcagtatgc aaacctccat gaaaatccag aagaaaaatt
     8101 ctatattcct aataatgttt atattattgg gacgatgaac gacattgacc gttctgttga
     8161 cacttttgat tttgctatgc gccggcgatt tactttctta gaaataaaag cagaagattc
     8221 agcacaaaat atgctgactc aagagaaaac aaaagaactt atgacgcgtt taaatgaggc
     8281 gatcgttgcc aaagaaaaag gtggattgtc aactgattac caaattggtg ctagttactt
     8341 tttaagtatc gataatggtg aggagacaat ggaacaatta tgggatacta agttatatcc
     8401 attattgaaa gactattttc atggtgaaca taaggcgaaa gaaaaattag aaatactaga
     8461 aaaggtatac tctaatgacg gtgatttaga tgaagctgaa acctctacac gataatcaag
     8521 aaatcaatga ctcagttgtg caacatccaa tcattacgaa cttagttaat cgtgacttaa
     8581 gtagtttaga gaaagaaaat tttattatct ttccgcaaca gttaaatcaa tccaaggatc
     8641 taagtgaaga taacttgata tttcaatcaa gaaatggaaa aatttggact tgtaatgtcg
     8701 taggtatcct tagcgattca acagatgaat taagaattca ttctagattc gctaatcaaa
     8761 tgaagcatga agatttcttt ttacgttata tgcttcagcg tgttttgaat tacaacgtga
     8821 ttaatgatca ttttagtgca tctaaaaaga tgacatacta cgatttgttg gtatttctct
     8881 ttccttatta tctgaatgaa gcaatgcgta aagggacata caaagaatat gtcaaacgag
     8941 aatacaataa tgccaatatc aagggagctg ttgatgttgc aaaacacatc cgttcgaatg
     9001 ttcctttcgt tggaaaagtt gcttatcgaa cacgagagtt tagctataat aatcacctaa
     9061 cccagcttat tcgtcatact attgaaaaaa tccaaaatga gtacgatttc ttacttagtg
     9121 gagatgagga tacgaaagaa aacgtggttc taatcaaaca aacaacacct ggttatgcta
     9181 gattaaatca atttaatatc cttcaagaga atatccttca tccagtcaaa catagttatt
     9241 acgaggagta ttcggctctt caacaaatct gtatccaaat tttatcggaa gaaaaatcag
     9301 gatttggaag tgataaaaat caaatacatg gaatcattat cgatgtgtct tggctatggg
     9361 aagaatacat tggaaaagtc actggttgga aacattatgg cagagataaa gggttagcaa
     9421 caatgcattt atttcaagag cccaaccggt ccccacgata tccagatttt acatttaata
     9481 atataccaat tgatacaaaa tacaaaaaac atcttgatac tagaaatgac tataatcagt
     9541 tagtcacata tattcatata atgaatttag accaagcaga tacaatgaaa ggcggctttc
     9601 tgcaacctac ttccgatccc cttcctgcaa atcatgggta t
//
LOCUS       DACKAC010000091         9154 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.092, whole genome shotgun sequence.
ACCESSION   DACKAC010000091 DACKAC010000000
VERSION     DACKAC010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9154
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.092"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            806..1006
                     /locus_tag="I0B46_002484"
                     /pseudo
     CDS             806..1006
                     /locus_tag="I0B46_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1493..1672
                     /locus_tag="I0B46_002485"
     CDS             1493..1672
                     /locus_tag="I0B46_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084736.1"
                     /translation="MTELTADMSDTWLQEYLFPEKQTTARGYLPVDWEYVHKELMKKN
                     MTLKLLHREYGVHGT"
     gene            complement(1718..2323)
                     /locus_tag="I0B46_002486"
     CDS             complement(1718..2323)
                     /locus_tag="I0B46_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAR2084737.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            2505..2840
                     /locus_tag="I0B46_002487"
                     /pseudo
     CDS             2505..2840
                     /locus_tag="I0B46_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3005..3232
                     /locus_tag="I0B46_002488"
                     /pseudo
     CDS             3005..3232
                     /locus_tag="I0B46_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            3358..4329
                     /locus_tag="I0B46_002489"
     CDS             3358..4329
                     /locus_tag="I0B46_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAR2084738.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICKENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            4469..5782
                     /locus_tag="I0B46_002490"
     CDS             4469..5782
                     /locus_tag="I0B46_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAR2084739.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQISGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            5825..6691
                     /locus_tag="I0B46_002491"
     CDS             5825..6691
                     /locus_tag="I0B46_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAR2084740.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLMPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            6688..7536
                     /locus_tag="I0B46_002492"
     CDS             6688..7536
                     /locus_tag="I0B46_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006639650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAR2084741.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            7550..9022
                     /locus_tag="I0B46_002493"
     CDS             7550..9022
                     /locus_tag="I0B46_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAR2084742.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            9039..>9154
                     /locus_tag="I0B46_002494"
     CDS             9039..>9154
                     /locus_tag="I0B46_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAR2084743.1"
                     /translation="MIYGSIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEET"
BASE COUNT         2920 a         1525 c         1871 g         2838 t
ORIGIN      
        1 tctatatttt gatatacaaa ttacagcttc agatactaag ttgcataaac cttttccgga
       61 ccccatacaa aaggcaattg atgaattggg cataaaaaat aagaaacgat ttatattggt
      121 gattcattat atgacatgca aagtgcgaaa gcttgtggga tagcatttgg tttagctaaa
      181 tggggagcca aaaatataga aaattttcaa agtgtagata taattattga ttctcctcaa
      241 gaaatacttg atttgttaga aaaatagaca aagagcgtga gataaggata gggtctcacg
      301 ctctttgttt ttcgccattt aaagattgaa atttttgcca tttaaaaaga aaagtgacag
      361 ataggaataa aaacttatta aatgtagtta tgatctgtta tatttcattt ctaatactag
      421 gtcttccaca agcagtgttt cctgaaatgt tttctggaga ctggggtatt gtcagctatt
      481 ctatattaac attgattgct atggttttag gtacttcatt ttataatgct tttgtaagta
      541 agcatgttga ggaatatgaa gtattaatgt acttaaaaga gaaaaaaaca agtaattaat
      601 atgagaacac cacgcatagt ttttattttg agatattcta gaatgaaaca cattaagaac
      661 gaaagcaaag caaaagacgc cagaacttct actaccttct aagttttttc atatttgttt
      721 gttatgctat acatagatct gcataattag acttgcgctg cccgttattg aagtttcagg
      781 cagcgctctt tttgggggat cgataatgga agaagaaaaa atatttgaga aacgctggca
      841 gctagcatca agtgaacaaa gagcaagata caataatttg atcacctcct accccactgt
      901 tgactagaca tataaggaaa agaaatactt actttggctg tgccagttag atatagatac
      961 ctttgaaaca tttgaagtga tcctagataa aattaaacag aattgatcag aaacaagcga
     1021 agccttctct cggttcgctt gttttgctat ttattagttg gctcagggca actctaagct
     1081 cgtttgaaag aaaaccgatg gataatcccc ttgtttgtac cactttagaa tacatggatg
     1141 tacgtattgc cattgactcc tattagttac tgaaaaaaaa tttaaacaag ttgagtattg
     1201 cagataaata gctccagtga ggtgaataac tgctccactc aaggcaatag atagctccat
     1261 ccagggcaac aaactgctcc actttcatat ttaatagatg aataaccttc tgtataatag
     1321 aaaggcatac ctttcggtat gacaacttat acagaatgtt attttattgg ttcactatcg
     1381 aaaaattttg gagctttatt tttccggtat ttctcaacga acgattagtt taactgtggg
     1441 aagctccaga catacgattc gagaagttac ttctcgagca gacaggaagg gggtgactga
     1501 actaaccgct gatatgagcg atacttggct gcaggaatat ctttttccgg aaaaacaaac
     1561 aacagcacga ggttaccttc ctgtagactg ggaatatgtt cataaagaac taatgaaaaa
     1621 gaatatgaca ttgaaactcc ttcatagaga atatggggta catggaacat agaaacgaaa
     1681 atgcacggta gaaaagcgaa tgaatatagt ataaattcta caaatcattc gctttcttta
     1741 ttttatatat ggttggtcga gagagaccat tttctttggc aatttctgca actggaactc
     1801 cttgatttaa catgtccacc acagttttaa agactaatcg tttttgcgga tctgttgagt
     1861 tctcagaata ttcaattggt cgccccttat atcgaccagc ttttttggcc aataatactc
     1921 cttgccgctg acgttcacgg attttctcac gttcgttttg tgcaatataa cttagcaatg
     1981 ataaaaacat atcgaacata gcagtatcta ggagttcatt tccagtatta aaatctaaaa
     2041 actgtgcatc tagtattttt aatgctactt ttttctgttt cataaaggta actgtatttt
     2101 ttatgtcttc ataatctctg cctaggcgat ccagtgatgc aacaatgaca caatcacctt
     2161 ctcggatata atttagtaat ttttgaaact caggtcgctg gatatcttta ccactggctt
     2221 tttcttggaa taatttatca atgttaaggt cttccatcat tgccaattgt cgatctaaat
     2281 tttgatcttg tgaagacact ctaatatatc caatttttgc cataaaaact ccttttaaaa
     2341 aatgatataa tttacatgta aaatttactt gtagttttac tgttatcata gcatttttaa
     2401 aaataaattt taagtctact tataatttac tttggaggtt ttgaatgaat gaaattaagc
     2461 gcaataaaaa ttcttttggt gtttatttag gagagcctag ctcggaacag ttaaataaag
     2521 gtgaagctcg tcatgctcta tgtcgaaata tattttatgg tagaaaaggc aaactttatc
     2581 aaacttatta cgacggtatg gaggaacagc tcaacgcatt aggattagta actaatgcta
     2641 tcatatattg gaattcacta tatttagaaa aagtctttga acaaattaaa acggaagggt
     2701 tcgactgttc cgaagaagtg ttacgcaagc aatttccatt aattagtgat cacattaatt
     2761 ttgttggcaa atatagtttc cgctatgaaa aagaattatc gaatggaata ctacgtccgt
     2821 taaatataga gaattattga gaatgttcgt cttttggttc ctaccgtgca ttttcgcacg
     2881 gatgttccag atactatttt tgaatgtata aaattgaaaa cacaagaata ctaataaatc
     2941 aacgattctt gtgtttttaa tttttcacta ccgtgcattt tcgtttctat gttccatgta
     3001 ccccaatctt gaaaaattaa ttaaagactt agataaagcc attgacgact tagtcgtggt
     3061 gctaccggag tatcaatgtt taaccagtat cccaggtatt ggtcctgttt aggctgctgg
     3121 aatactcgct gaaattggtc caattgaacg tttttctgat gaaataaaat tgacaaagta
     3181 tgccggccta tattggcgac agagtcaatc cggtaaatct gaatacgaaa atttttttac
     3241 gaaaagcttc acgtctaata tctattttaa aataaaaata ttcctgctaa tttggaatcg
     3301 tttccttctt ttggtataat aatacctgaa attcttttga gaggagttgt tcgtgaagtg
     3361 aaaactacta tgaaggatgt tgctgcgtta gcaggagttg gtgtgggtac ggtatctaga
     3421 gtaataaatg gagtaaaggt gaaagaggtt accaaagaaa aagtaatggc tgcaattagt
     3481 gagcttaatt atgagcctga tgaatatgcc agaggtatga aaacgaatag tagcaataca
     3541 atagctctta ttttacctac gatttggcat cccttctttt ccgagtttgc ctactatgta
     3601 gaggaagctc tcagccaaaa aaattataag ctttttttgt gtaatgcgaa cggcagtgcg
     3661 gaaaaagaaa aagaatatat ccaaatggtc aagcagagta aagtagacgg aattataggt
     3721 attacctatt cagacattga taaatatgta tcatcaaatt taccatttgt cagtatagac
     3781 cgccattttt cggaacaggt ttcttatgta acggcagata attatttagg tgggcaaatt
     3841 gccgctcaag aactacttac tcgtaactgt aaaaacttgt gctatgttgg gggtgtttca
     3901 ccttacccta acgaaacaaa caatcgtaaa gctggttttt acaatatttg taaggaaaat
     3961 aaaatagctg tgaaggtatt ggatatgcct gaaccaatta gcaatttaga ggaacaattg
     4021 ctcgcttttt tttcggataa tcctgaaatt gatagcattt ttaccatcaa tgatttcatg
     4081 gctttggatg tgataaaagc ccaaaaaaaa ctagggaagg aagcacctaa ggattttcaa
     4141 attattggtt ttgatggagt caagctatcg ttgcaaaaag actatttact atcaacgatt
     4201 gtacagccga tagaggaaat ggccaaggtt tcagtagaaa cactcatggc gatgatttta
     4261 aatgaagaac cgcctaagcg tgtggtagta ccggttttct ttcacgctgg agagacgact
     4321 aaagagtaaa aaaccggttt ataacgattt ttttgagata ggttgacaga aagtgatttc
     4381 aatgatatct taaaaccaat taattggaaa cgttaccaat taattgaaaa ataaaacgga
     4441 aacgtttcaa aaaataggag gaacatcgat gaaaaagaga atgttagcaa ttgcagcaat
     4501 tttagcatta acagctggag gacttgcagg atgtggcagt aaggagaaaa gttcaggtag
     4561 cagtgatcca ggagaaatta agatttgggt tcagttttct gatgaaacgc ctgaaggaaa
     4621 agcttgggca gagattgtga acaattttaa tgatgaatac gagggtgaat ataaagctgt
     4681 tactgagtat attcctcgta gcggaagcgg cggtggatat gaggataaag taaatgctgc
     4741 aattacaacc aatacccttc ctgatgttat tacgttagat ggaccgaata ctgcagctta
     4801 tgctcattca ggagtcattg ctccattaga tgattatttg aaagatgcgg acatggatga
     4861 tgtattagac agcattattc agcaaggaac ctacgatggc aaaatgtatg cgtttggttt
     4921 ctcagagtcc agtgtgggtg tctattacaa taagtcgctg ttcaaagatg ctggaattac
     4981 agaagatcaa ttgccgacgt tagaagatcc ttggacttgg acggaatttc aagcaatttg
     5041 ccaaaagctg actgataaat acaaaacgcc tgcaattgat atgcaaatgg cgtcgggaga
     5101 cgaaatgctg acctacgcat atacaccatt tatatggtcg aatggcggcg atgtcgttaa
     5161 tgctgatgga accaaagccg aagactattt taacggtaaa aaatccgttg aagcgttgga
     5221 atttattcaa gaattggttg aaaaaggtta caccacaatc agtcctattg aaaaaggatt
     5281 tgaaactggc aaatatgcca tgatgctgag cggttcttgg acaatggcag atttagagga
     5341 aaactatcaa gagattgatt atggtatttt gccttacccc gtttcagatg caactgggga
     5401 gttagtttca ccaaccggaa gctggcagtt agcaatgact gagaaaacag agaaaaagga
     5461 tgcttctgcg gcctttatta agtttgcaac cagtacagaa tctagtcgta ttatgagttt
     5521 agggaacagt gttctaccta tcagaaattc aacagttgat ttaattaaag atgaagtgtc
     5581 ggaaggaatg caggtattaa ttaagcaaaa ccaaatttct ggacatgccc gcccagtggt
     5641 tgtagcttat ccgcaagtat ctagagcatt ccaacaagca gttcaagata ttagctactt
     5701 cgaggaaaac ccagatgtac aaaaagtagc agatagtcgc gctgctgaaa tgcaacaggc
     5761 aattgatact tcactaaaat agaagtttct aaagggggcc aatcaagtgt taaaaaaacc
     5821 gacaatgaaa gacaatttga taggttacgg ctttctagcg ccagctttgc tgctgctgac
     5881 gatttttctg atcatacccg taatcatggt aatttattat gcctttactg attattattt
     5941 gttaatgcca gatgctagac aatttatcgg cttagaaaat tttaagcgac tgttcgctga
     6001 tccaattttt gtaaaaagta tttggaacac ggctaaattt gttgtttgga ttattccggt
     6061 ccaaataggg gcagctttag gaatggcact tattatcaac aaaaatcgta aaggaaatat
     6121 cttttttaaa gtagcctttt ttgcgccagt cgtgatgtcc ttagtcgtta tttcgatttt
     6181 atggttgtac ttgttaaatc ctaatgaagg tctggttaat acgctatttg caaagattgg
     6241 aattgcttcg cagccttttt tgactagtcc caaacaagca atgtatacgc ttgtgttcgt
     6301 atcggcatgg caaggtgcag gatatcagat gctgctgttt ttagggggaa tgcaaaatat
     6361 tccgcaagac gtttatgagg cagccgaaat tgatggtttt tctaagtttc agcaatttcg
     6421 ctatatcacc atgccattat taaagcccac ggcgattttt attttactta ccacattgat
     6481 ttcggccttt aaactaatca ttcagccaat ggtaatgact caaggtggac caatgaattc
     6541 tactatgaca atggtttatt acatttacca aactggattt acagaccgta tggtaggcta
     6601 ctccagctcc attgcgttaa tctttaccac tatgattggt ttaattacaa ttgctcaaag
     6661 aaaattggta aaggaggatg atccagcatg accaagaaat ttggaccgct aatgatcgta
     6721 gaatattgtt tgctgttcct tttagcggca ttattcattt tcccgatgct gtggatggta
     6781 gtttcctcta tgaagcctga agcagatgtc tatcaaaatt tagcttcctt caaagctttt
     6841 ttacctagtt tgaatccagc taattggttt aaaacttatc aagaggttat ttcccggttc
     6901 agtgtgggaa cttatttatt aaacagtatt ttttatggtt taacctttgc ctgtgggtca
     6961 atcattgtaa actctttggc gggttttgcc tttgcgaagg ttaatttttc aggcaagaaa
     7021 attatctttg gagctttgtt agctctgctg attgtcccga tggagacggt tttaattccg
     7081 caatttacca tcgttaattc actaggctta gtgaatactc gcttggcagt gattttacca
     7141 gcgatggcca gcgcgtttaa tatctatctg tttagaaatt tctttatcgc gattccagaa
     7201 gaaatcattg aatcagcgaa attagatggt gcaaatattg ttaaaatttt ctttcacatc
     7261 atgctgccaa tgtctaaacc agccatagcc acagtaggtg tcttatcctt tatctccagc
     7321 tggaatgatt atatttggcc gttaatggtc ttaactgaca gcagcaaatt ttcaatgcaa
     7381 gtggcaatta ctacgatcaa tactacacag ccagtttata tcaatcaagt gatggctgtt
     7441 ttaaccattg caacgattcc actgattatt gtttatatcg ttgcacaaaa atatattctg
     7501 cagggacttg gcggttctgg aacgggtata aaataggagt gaatagtgaa tgttgcaaaa
     7561 acttttaaag gccaatgagt ttattgagaa aaatgctcaa acagtcaatc aagaataccg
     7621 gaataaattt catttgatgg ctccgattgg ctggatgaat gatccaaatg gttttgttta
     7681 ttatcaagag gagtatcact tgttttatca atattatcct tacgacagca tctggggacc
     7741 aatgcattgg ggccatgcta aaagcaagga tctaattcac tgggagcatt tgccagtagc
     7801 actggcacca ggtgaaagct atgacaagga aggctgtttt tccggcagtg ccatagaaaa
     7861 ggatggcaag ctgtatttga tttatacagg acacaatgtt gtggaagggc aagttcgaca
     7921 agttcagtgt ttggcagttt cagaagatgg cattcacttt gaaaaatatg ccggaaatcc
     7981 aatcatcgca gaagatcact tagcttctgt tgcaacgacc gaagatttcc gcgatcccaa
     8041 agtgtttcag cgaaaagaag actattttac tgtagtcgct actaaaactg cagatgctcg
     8101 tggacaaatt gtgatgttcc attccaaaga tttactaact tgggagtttt attccgtatt
     8161 gctggaggga actccagaac aaggcattgt atgggaatgc ccagacttgt ttgaactaga
     8221 tggaaaagaa gtcttgattc tttctcctat tcaaatgact ccacaaggca atgcttacca
     8281 aaatacgaac tcaactgttg ctttcatcgg tactgtcgat tggcaaacag gcaaatttct
     8341 agtagagaat tttcatgaaa tcgatggcgg tttagatttt tatgcacccc agacctgtga
     8401 aaacgaagca ggccaaagaa taatgattgc ttggatgcaa atgtggcagc gtaaaatacc
     8461 tacgaatgat ctgggccatg gttggtcagg ttccatgacc ttgcctagag agctgagggt
     8521 ggctgataat caattgttgc aacggccagt ttcatcaatt tatcaagtaa ttgatccgca
     8581 ggaaaggatt gaatgggcga agactgaaga gaatcgcttc ttactgaagg acttagtata
     8641 tgaccagcac tatttacgct tggaaatcaa tttaaaacag gcaacgaaac tagaacttca
     8701 ctatgcgaag ggacccgacc atacattact gcttaactat gatgttgaac gggagtgctt
     8761 tactgcttcc agggaacaca tgccgtatga tttatcagga gctgaaaagg aagtgttgaa
     8821 aaagcgcagt gtgacgattc ctttggaaaa taattgtcta accatcgaaa ttttccgtga
     8881 tacttcagct attgagcttt ttttaaatga tcgtctgact atgagtacaa ctttttatga
     8941 gacagaaaaa ggacaggatt ttcttttgct ggcagacagt gaagtaacct tttcattgga
     9001 aagcggctta attaatctat agaagggaga gctcagtgat gatttatggt tcaatcgaag
     9061 caggcggcac aaaatttgtg tgtgccattg gcgatgaaga aatgacgatc aaggaaagag
     9121 tgagctttcc gacaacgaca cctgaggaaa caat
//
LOCUS       DACKAC010000092         8424 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.094, whole genome shotgun sequence.
ACCESSION   DACKAC010000092 DACKAC010000000
VERSION     DACKAC010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.094"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            471..2108
                     /locus_tag="I0B46_002495"
     CDS             471..2108
                     /locus_tag="I0B46_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAR2084744.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSMGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(2253..2993)
                     /gene="truA"
                     /locus_tag="I0B46_002496"
     CDS             complement(2253..2993)
                     /gene="truA"
                     /locus_tag="I0B46_002496"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAR2084745.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(3184..3819)
                     /locus_tag="I0B46_002497"
     CDS             complement(3184..3819)
                     /locus_tag="I0B46_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAR2084746.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(3966..5207)
                     /gene="sstT"
                     /locus_tag="I0B46_002498"
     CDS             complement(3966..5207)
                     /gene="sstT"
                     /locus_tag="I0B46_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAR2084747.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            5405..6401
                     /locus_tag="I0B46_002499"
                     /pseudo
     CDS             5405..6401
                     /locus_tag="I0B46_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6417..>8424
                     /locus_tag="I0B46_002500"
     CDS             6417..>8424
                     /locus_tag="I0B46_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAR2084748.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKILTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQK"
BASE COUNT         2785 a         1547 c         1293 g         2799 t
ORIGIN      
        1 aactaatcag aatcgtacaa atatttaata accaaatata tagaacataa cttaatatta
       61 agttatttga aattccataa aaaacgactg caggaattat tgtaagaaaa ggataaaaat
      121 agttcagtcc atctccataa ttgttaaatg ttgtatagtt gattggccct tcaaaaatag
      181 aagataaccc tttgattcgt gaaagatgaa aagataaatc acctgttgta taaatttctt
      241 gagcatttct cataaaattt actgaatata aagtactaag taagataaga actactaaaa
      301 gaaatgcttt tttacttatt ttcatagatt tgttgttctc cttttttaat tctattttca
      361 gtatactaga attgtctttt tttaataagt tttgcgttaa atttttcata gcactagtat
      421 gatatactag aaaagaatca atttttatta cttttaaata ggagaaaaaa atgaaaaaaa
      481 acaaaaacca aataatagat atcattttta tgttatttct aagttttgct tacgtcatcc
      541 ctctattcat gtctatggga atctatcata gtgtcaatca ggatacttac tttcatttat
      601 ctcgaatcat aggactagat aatgtatgga gttctccagt aaacttcaat aacttcgatc
      661 atcatggaac aatgatgaat attttttatc catggcttac tctttatcca gcttttttat
      721 tttataaaat gatgggaaat cttgttttaa gttataatat ctactatttt tttatcactt
      781 ttttaacaat ggtcgtttct tatttttcta tgaaacaaat aaaaaacaat cgttatattt
      841 cgctgttatt ttcgattatt tatacatttt ccgcctatcg tgccattgac atattccatc
      901 gtgcttcatt gggagaagct gtcgctttaa catttttgcc tttgatacta atggggtgtt
      961 acgaaattta tattagagat taccaaaaat ggtactggtt gtctattggc atgacgctag
     1021 tagtgtatac tcatttactt tctgtcgcta tggtttctgt atttattgga ggaacgttgt
     1081 ttctttcttt ttatttttgg gaccaaaaaa tcgctagact cttaagtctt ttaaaagcta
     1141 ccgtcctcac ctttttttta agtgcgggat tcttaattcc ttttattcaa caatctagag
     1201 cacaagaatt gaaagtccca ctgggaaaag aattaagtgg tatggctcct agtgatatgc
     1261 tgacacacat tttaaataat aactataaca actatactat tggattattc ttatttctcg
     1321 gattgattgg tgcttttatt tttataaaaa aattaactgc tgatgattta tttattttct
     1381 ttcttggtgt atttgttctt ttttgttcaa ccaatttatt tccttggcaa ctttttaacc
     1441 atacaccagt taagagtcta caatttgtct ggcggttaaa tggtttttct tctttattta
     1501 tagcctatac gatgagtata gtgatctatt atattttttc tactaaaaat aattcatgga
     1561 aaataatggt ctttacgttt cttgcactaa tattgcatct ctcaggagta agcaattcta
     1621 tccatgctaa caaagattca ttaacactta tagcacctga agacgctgtt gctatagctg
     1681 aaaattacaa tcatactgat tatgcaaaca aagagtcaat ctatcatcct gatatgatta
     1741 ataatgatca atatttacta ggtaaccagg aaataaatcc aacaactcat tttatgagta
     1801 acaaacttac aattgaatta gataattcga ataataaaaa tactgtcttg acgacaccta
     1861 tttatcgtta taaagggcaa gtggcttcta ttaatggcaa actagtacaa acaaaattat
     1921 ctaagtttgg cacaactgaa ttaactattc ctccaggtat aaataaagtc gttattactt
     1981 atcagtacac aaaactagca attgcttcaa gatatttatc tatcgttact ttgattttat
     2041 ttttacttta tcgatttact ttttctaaac aaaaacaacc gagaagagaa gtacaacatt
     2101 ctcactagat ttttatttaa gaaagacttt ttagctgtac acataaacag gctaaaaagt
     2161 ctttttttat atttctttaa tttgcttcat acattattta acatcgatat ttatactgtg
     2221 aaacatgcgt ttcatccatt atcaaagtat ttttatcgat aataaacttc atccaaaaac
     2281 agaccttgtg cagggacggt ttctcctgca tgttgccgca ctttgctttc aaatatttca
     2341 tcgattgttt ctatcggcat ggcacctgtg ccgatttcta gtaatgtccc catgataatc
     2401 cgcaccattt tatataagaa tccgtctccg acaaaagtaa aatgaagcat ttctcccttt
     2461 cgttcgatag ataattcatc aatcgtacgc actgtggatt tttttgattt tttcaatgca
     2521 gaaaaaccaa tgaaatcatg tgtacccact aatttttcgc aagctgcatt cagcttgtcc
     2581 atatccaact cttgagggaa ataaaagctg tggtttcgct caaatgctga aggaatcacg
     2641 ttgttccaaa cataatagct gtattttttg cccaccgcat tatatcttga atggaagcgc
     2701 tcaggcattt cttcgattct tttgatgaca atatcccgcg gaaggtaacg attcataaag
     2761 tcgatcattt ctgccagtcc aatttctgtg tttgttttaa aatttgctac ttgtcctcgc
     2821 gcatgggtgc cagcatctgt tcttcctgat ccaataattt ctatctcttc atttgtcatc
     2881 tgatgcaata ctttttcgat ttttccttga atggttttat cgtcatctcc aagtctttgc
     2941 catcctgaat atctttttcc gtcatattcg attgtcaact tgatgtttct catattttag
     3001 tggctcctct gtcttccatt tggtcttctc tctacctttt taagattgta gcacaaaata
     3061 tagctccttt cttctctaaa acagaagcta tacaagaaaa ctgtggaaat atatatttca
     3121 gaaaaacaaa aaacagccac cggatgaagt atccgctgac tgttcaattt gacggctcaa
     3181 gttttactgt ttgagatgca agatactcaa ccaggcaatg ggcacataat taaagaaaaa
     3241 actgattctt tggcaaaaac ctattttatt cagtaatggc caagaattta tagaaggaat
     3301 tcttgccagt ccataaatcg ccgcaaaaag caaactgatc acagacagtc caaggtataa
     3361 gtggcttacg tccatttgcc cattttttcg atagatcaag aaaagaagaa ctggaaacaa
     3421 taaaaaacca ctgtaaccta aacctgatcc gatattatgg acccatgtcg agacattcca
     3481 cgaagattca tgggtattga tactgaacaa acctgtaaag atacaatcac ctataccata
     3541 gaacgcgatg gctgctgtaa ctagggtagc taacacacga gatgtactta caaatgcagc
     3601 atacaatgca ggcaatgaca ggatgaaaaa gatccctgaa accacagacc aaataagaaa
     3661 cgcttttctt acaggactgg aaacctctcc aaatacactg atcacgctct tcatttgatc
     3721 taattcagga taatacaatc ctaatacgta cggagtcaaa aaatcactca gcacgcctaa
     3781 taataaaaag aaaaaaccaa atttacgtaa aaaatccatg atacgcctcc aataacaatt
     3841 acctatgctt ttaattgtta ttataagcga ctttatcttg atttaacaga aacctctatt
     3901 tacacgaaca tctatataac tcattaaaaa ctcgaatcat tttaaaaata ctaaatatct
     3961 aacgttcaac gttcacttaa tccatctaaa caatctttca atttgatttt ttccccactt
     4021 cttttacgcg cgcctagctc tcctattgca gtaaacaaca gatcacttga cgaatttaag
     4081 gcagtttcca ttgaatcctg gataacaccg ataataaatc caataccgac gatttgcatc
     4141 gcgacatcat tagagatacc aaacaaacta catgctaatg gaatcaataa cagtgaaccg
     4201 cccgcaacac ctgaagctcc acaagcagct aaagcagaca aaatacataa aaggactttc
     4261 aaaacaaaag gtgcttcaat ccctaaagaa tgtacagcag tcaacgtcat caaggaaatg
     4321 gtaatcgcgg caccgcccat attgatcgta gcacctaacg ggatcgatat ggaataagac
     4381 tcttttgtca atttcaatct ttcagccaac atcatgttta tcgggatatt tgcggcagaa
     4441 cttcgggtaa agaaagcggg aagtgcgctt tctttcaaac aaaagaaaat caatggatag
     4501 ggattttctt taataaacaa aaacgtgagg aatgggtaca caactaatgc gacaaacagc
     4561 atcgtaccga tcaaaacaag taaaagctgc agatactctg ccaatccagc tattccagtc
     4621 gtagcaattg attgataaac caatcctaaa atccctatcg gagcaaatgc aataatcact
     4681 tgtacgactt gtgacaacgc tatagatatc tgatccacca actcttttgt cgattctgaa
     4741 cgtgctcgaa aagctagtcc caacaataca gcccagaata agacgccaag gtaattcgca
     4801 tcaataagag attgaacagg attttgtgtg acattcgtca acatagtaga taaaacactt
     4861 tccaaacttt gcggtgcttt ttcagatact gtttcttgta aagtaatagg aacagtaaaa
     4921 attgaactta caataactgc tacaagtgca gaaagcaatg ttcccactaa atataagatc
     4981 aaaataggct tcacgaatgt cttattccca attttatgct tggcaattga cgccatcgtc
     5041 aagaaaaaaa ccagaatagg tgcgattgcc ttcaagcaac tgacaaacaa cgttcccaat
     5101 attgaaataa atgtccatgt ggggaaaaag ataccaaaca atattccgac taaaatacca
     5161 acaacaatac gttgaactaa cgacatatcc aaaaacttct ctaacaaatg accacccttt
     5221 cacaatttta acaatcattg tatcatttta tgcaaagaaa ctaaaggtat aattttctaa
     5281 aaattctaac aatacaaaca accatttctt gctaattttg tactttttct gtctacacgt
     5341 tgttttttga gatattctaa gttaggatgt tattaatggg caacttatca ggaaacgttt
     5401 tcttatgttt ttgtccaatt tttttgtgag gtgtttattc ttgaaaaaaa taaagaagga
     5461 gagttatggc ttcctgacaa tctttgtcgc ttgtataatg atggttttac agcgtatatt
     5521 gatttcagca gtcactattc ctacatatgc aacaaaccct ggtcgattct acatctatac
     5581 atttttacaa acttccgttg ttattggtgc aaatttgatc ccactttaca tgggttacca
     5641 atatcaaaaa atcgcccgtt taaaagtttt acattattta agtcgttttg cctttatatt
     5701 cctaatagcc acactagttt gcaatatctt ttttttacgt acaagctggc agcttgaata
     5761 ttcgtgatta ttggcttatc ctaacaccta tcagtcagaa ttactttact tatgctgttt
     5821 cttgtacttt gcttttttgt agtattcctt atacagttgg atttttaaat aaattatcga
     5881 aaacaacaat taaatatcta ctattatttt taacaattgg attagttggc tgctctactc
     5941 tatttaataa agacccgttt gcattacaga atggacagag tattttatgg attttttatt
     6001 tatttttaat gggttatggt ttgaaagaat ggaactggaa agaaaaggtc cgctataagc
     6061 ttcctcaagt atttatcagc ttgattgttt tattcggttt gattattttg atgacacaaa
     6121 tttcactcat catcagagga aatacagata cagcgctgag gttttctaaa ccatattctc
     6181 tcttcgcaat gtattatact ttctcttcgt ttttattttt agaaatgcta tctgataaat
     6241 taggtataaa aggaagagga aattttcttg ctgtattttt gattgtcact caagttatat
     6301 gtaatttccc tttagtttct tatcgcgtat ctacatttat gaaacgtgaa tttccaaact
     6361 cagggaaggc atggctgata aatatcgggc agttttttat ttgctattta ttagcagtgt
     6421 ccctcctagt tatcttttta ttttatatac aaaaaatccg tttcattcat cgtttcatcg
     6481 aatacttcgc ttttgattcg attgaacaat taactcagcg ttggaaaaaa agaattcatt
     6541 ggctttttgt tcataaaagt tattttttag ttgcagcatt tttttactgc tttaccttcg
     6601 tgcagatttt cgttttagaa ccaaaagaag gttggaaaga aactatccag gttgccttaa
     6661 aaatatttac tcagcgacaa gcacccatga tcctgacaat catcattatt atgggatttt
     6721 tcttcttact attgttgatt tccaatcgtt tttggtacgc cttgactgct accttgatca
     6781 ttaacttact gctcactatt tcgacagtaa tcaaaatgga aatgcgggaa gagcctgtct
     6841 ttccttcaga tttaaaaata ctgaccggat tgtcagaact attatcgatg gttagcccag
     6901 tcttattaat cgtgggcgga ttgatccttc tcctattatt gattactagt attattgtac
     6961 aacgtagact acaaaaacag tattccttga agattcattg gaaacgtcga tttatcagta
     7021 tcgctgtatt attaggatgt ttttcgggtg tctttttcat taatcacaaa aattcgcctt
     7081 ctttcttgct gtttaattta tttaaagtaa acaagacttt ctttaaccag caaggcgccg
     7141 taaaagataa cggaccagtc attcaattcc taaacaatat cgacattaaa atcatggaag
     7201 aacctgctgg ctattcgaaa gaaaaaatta atggaataat gaaaaagtac aacacaaagg
     7261 cagatgaaat caaccaaaca agaagtgact ggttaaacaa tcagacgatc gttctcaatt
     7321 taagtgaaag cttcagtgat cctgcacgtg tccctaatct gacagtacct acagacccta
     7381 ttccttacat caaaagcatt ataacagaag gaacaggagg attgatgtta tccgtcggtt
     7441 atggtggcgg aacagctaac atggaatggg aaggactgac aggcttgagt atcagtaacc
     7501 tgtctgcttc gttagttaca ccttataccc agctagtgga gcgtcagaaa ataagtccaa
     7561 atattaccga tctattcgat gaaaaaatag ccatccatcc ttttacagct gctctttatc
     7621 gcagaaaaca agtgtttgat aaatttggtt ttgacaggtt ctattattta gacagtccgg
     7681 ataaattaac ttatacggat aaaatagaac aaagcccacg catatcggat gattcagcat
     7741 acaaagaaac cttgaaagct ttacaaagca atacagaaac gtcacaattc atccaattat
     7801 ccactatgca aaaccatatg ccttatgatg attactatag tgaattgaac tacactgctg
     7861 aaggaactgc tgtcattcct aatagaagaa aagaattaca aacttatatg caaggccttc
     7921 actataccga tacagcagta aaaaaattca tccaagaaat cgacaaaatc gataaaccga
     7981 ttacctttgt tttttatggt gaccatttac ccgcaatcta tagtgggaat gattcgaaaa
     8041 aatatggatt agaacagcac cagacagatt atttcattta taacaacaag tatagtcgtg
     8101 aacaagcagc tacgttaaat aaagaagttg ttgctcctaa taactttggt gctatggctc
     8161 ttgagcatgc gaatatcaaa gtaactcctt actatgcgtt gttgacagaa gtatctaatc
     8221 atctacctgc attaacaatt gatcctacag aaagcttgtc taatcgtttt aatggaaaac
     8281 aggtctttgt tacagaggat agtcaaatta tccaagaaaa aaatttaact gagaaacaaa
     8341 aagaaattct agaagattat cgtctgatac aatatgatct tgttgcaggt aaccagtaca
     8401 gtgcaacatg ggcgacacaa aaaa
//
LOCUS       DACKAC010000093         8296 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.095, whole genome shotgun sequence.
ACCESSION   DACKAC010000093 DACKAC010000000
VERSION     DACKAC010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.095"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(51..1517)
                     /locus_tag="I0B46_002501"
     CDS             complement(51..1517)
                     /locus_tag="I0B46_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAR2084749.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1904..2500
                     /locus_tag="I0B46_002502"
     CDS             1904..2500
                     /locus_tag="I0B46_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAR2084750.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(2558..3040)
                     /locus_tag="I0B46_002503"
     CDS             complement(2558..3040)
                     /locus_tag="I0B46_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084751.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3146..3343)
                     /locus_tag="I0B46_002504"
     CDS             complement(3146..3343)
                     /locus_tag="I0B46_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084752.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3638..4396
                     /locus_tag="I0B46_002505"
     CDS             3638..4396
                     /locus_tag="I0B46_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAR2084753.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4456..4884
                     /locus_tag="I0B46_002506"
     CDS             4456..4884
                     /locus_tag="I0B46_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084754.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4930..5002
                     /locus_tag="I0B46_002507"
     tRNA            4930..5002
                     /locus_tag="I0B46_002507"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4963..4965,aa:Gln,seq:ctg)
     gene            complement(5152..5523)
                     /locus_tag="I0B46_002508"
     CDS             complement(5152..5523)
                     /locus_tag="I0B46_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAR2084755.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5738..5923
                     /locus_tag="I0B46_002509"
     CDS             5738..5923
                     /locus_tag="I0B46_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084756.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(6006..6194)
                     /locus_tag="I0B46_002510"
     CDS             complement(6006..6194)
                     /locus_tag="I0B46_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084757.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6218..6481)
                     /locus_tag="I0B46_002511"
     CDS             complement(6218..6481)
                     /locus_tag="I0B46_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084758.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            6639..6896
                     /locus_tag="I0B46_002512"
     CDS             6639..6896
                     /locus_tag="I0B46_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAR2084759.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(6967..7158)
                     /locus_tag="I0B46_002513"
     CDS             complement(6967..7158)
                     /locus_tag="I0B46_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084760.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(7299..7619)
                     /locus_tag="I0B46_002514"
     CDS             complement(7299..7619)
                     /locus_tag="I0B46_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084761.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(7642..8076)
                     /locus_tag="I0B46_002515"
     CDS             complement(7642..8076)
                     /locus_tag="I0B46_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAR2084762.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
BASE COUNT         2633 a         1635 c         1314 g         2714 t
ORIGIN      
        1 aaaaacagga actgctgcaa ataagcaatt cctgttttta tgttcattga ttaatgaata
       61 gagcccatta gttttttgat tggttctttc acagtaaata gcaagatacc accgacgatt
      121 gctactgcac cgataatacc gaagtaagca gattctgttc ccggtgtgta gaagcgtgca
      181 atttgtgcat tgattgcttg tgaagaagca tcagctaaga accaaatagc gattgtttgt
      241 gattcaaatg ctcgtggtgc taattttgtt gtcacagaca aaccaactgg tgaaagacac
      301 aattcagcaa taaccacgat gaagaaactt cccaacaacc acaatggact tacttttcca
      361 cttgtaccat aaagcatacc cggcaacatc aatagcaagt acgataatcc tgcaaataaa
      421 agtccaagag agaatttcac tactgtagat ggttgacgtt caccaagttt cgtccacatc
      481 gttacgaata ctggtgtcag aataaagatg aaaattggat tcaatgattg ataccagctt
      541 gcttggatcg ttacaccaaa tagcgtagat tgtgtacgtt cactagcaaa taaagctaag
      601 atagatgaac cttgttcttg taatgaccag aaaatgattg ctgcaaggaa taatgggata
      661 taagcccata ctcttggttt ttcttcagca gttacatctt ttgaagtcag cattttgata
      721 aagtaataca acggtaagat aatccccaat acactgattg tattgacaaa aaagtcaatt
      781 gtcagatgac cagttagttg tgccccgccg aatactgcag cgataacaac aagtgcgatg
      841 atcaagttac ggatgaatcc tttacgttct tcagttgtca aaggattcgg cggtgttgta
      901 cccactccgt ctaatgtctt gcgtccttgg atgatgtatt gtaataatcc aaagaacatc
      961 ccaatagcag ctaaagagaa ccctaaatga tagttatatt cttgaccgat tgtaccaacg
     1021 attagtggtg ctaataatgc ccctaagttg acacccatgt aaaaaatcga aaatcctgca
     1081 tctcttcgca agtcagtctt tgaataaaga tgtccgacca tgccagaaac atttggtttc
     1141 aaaaaggcgg ttccgataat aatcaatgcc atcgaaacaa acaatgtact tacaccaaat
     1201 ggtaaagcca agacgatgtg gccaagcata atgaaaactc caccgaagaa tacagtgcgt
     1261 tttgctccta acactcggtc agatacccat ccgccgacga tactagacat gtagatcaaa
     1321 gatccgtaga tcgacatgat agctaatgct gttgctcgtg gtaatccaag tcccccgttg
     1381 gcaactgtat cgtacatata atagataagt aatgcacgca taccgtaata actaaatcgt
     1441 tcccacatct cagtgaagaa caaagttgac aatcccttcg gctgcccgaa gaagcccttg
     1501 tcttgctgtt tttccataat ctatacccca attctttaaa ttttcagaaa atattataca
     1561 tctttaccga aaaatttaac taaaaaactt atttaagaaa tataaactaa ttttctataa
     1621 aagcaagtaa tttttaatgg caatatcata aaaaaaagaa gatagagatt taataattat
     1681 atatcagctg tttttttcaa ataagcaaaa attcctaaaa cacaacaatg aaagcgataa
     1741 ctaagaaaat ccttacataa cgatttttct tattaaaatt gttttttgaa cttcgcttat
     1801 tttttgtgag catatctgtt tacttacttt ttgtaaggtg ctatacttca tttcgtaatc
     1861 aattaaaaaa atgcaaaacg caaatcaaag gagaaatttt attatgacaa cttttactga
     1921 cactttgaaa aaccgccgtt caatctatca tctaggacgt aatgtttcac tttctaatga
     1981 agaattaaca actttgatca aagaagcaat taaagaaagc ccaactgctt tcaatgctca
     2041 atcaacacgc gcagttatcc ttttcggaga tgcacatgaa aaactttggg aaatcactga
     2101 agaagcactt cgcccattga ctccagcaga agctttccca aatacacaaa acaaattagc
     2161 tggtttcaaa aatggttatg gtacagtatt attcttcaaa gatactgacg tggtaaaagg
     2221 tctacaagaa caatttgaat tatatgcaga caacttccca gattggtcag aacaatcaaa
     2281 cggaatcgca acagctaata cttgggtagc attagttgat aaaggattgg gcgcaaactt
     2341 gcaacattac aacccagtta ttgacgaagc agttgcaaaa gaatggaata tcccttcaaa
     2401 ctggaaacta cgttcgcaat tagtattcgg ttcaccagaa acaccggctg gggaaaaaga
     2461 atacatgaac gatgccgatc gcttccgcgt atttggataa ttttatttga aataaaaaac
     2521 agaggttgtg acaaacatct tgtcgcaacc tctgttttta ttctggatca atcataatat
     2581 caatctctgc ctcatcgttt aagcgttggg acaaaaatgg aactaattct tctaatgatg
     2641 cagaaaattt cagcatcggt cctcgccatc cgtttcggaa aaaattcatt tttacgattt
     2701 tagaaccttt tggctgatat aatacaattt tattgatctc agaaaaagga acaccttttt
     2761 tgtcaaagct attcaaaaca agtccttctt ctgtgattcc tctactgtcc aacaaaaaac
     2821 taataaaaat cattgctgac aagatccctt ttgccaattc atctgctgta ggatgactga
     2881 tgagtgtata ccaaatgact agtgacacta agaagggtat caccaagtag acccatcgat
     2941 ttcgcacagt ccgataaata atttcttttc gaaccagcca aagaaataaa aacatcacaa
     3001 caacgaataa aaacaataga accgtaaaga atgttaccat gacaactcgc ctcgcttttt
     3061 cttctccaga aaataggaaa agaatactga tttaccttct caaagaaatt ggacttgcag
     3121 cacgccgcac gtccaattgt tttctttatt caaatgtatc atattttgca acaaccatgc
     3181 ttgctttttc atctaataat tcttggtcct ctgcagcaca ttgggcaact ttgaccacgg
     3241 cgcagtttgt catttttgat acaacttcac caatgaccgt ttttgtaaaa ccaattggtt
     3301 tgcatgcata ttgttctcct aaattaatca tttcttccgt catcaaaatc ctcctaaact
     3361 gctggaactt ttctccacat taccactgct atttctttgg agatttaact cattgatttt
     3421 tatttacagt taaaaatcaa ccttacaggc attatagcgt actgttttcg ttttcacaat
     3481 taaaacagct gttgtattta gaagaatttt tttcaaaaaa ttaaaacata ttccttaaaa
     3541 actaattatt tcctacatat taaaatataa tacttccatt tccaataaaa atacatttat
     3601 aataggaaag ataaacagct gaaaagggag atttcaaatg aagaaaaaaa cgttcaaaaa
     3661 actattgttt ctagggggca cagctgctct tttattaagc ggtacaagtt attacctaca
     3721 tatccattct ccagaacata caatgaacac tgcacaaaaa agttcaacac aaaaaacgaa
     3781 aaagacaaaa gagaaagcag aagttttact agatgttcca ttagagagcc agtttgattc
     3841 tccttcttta gaaaatggct gtgaagtcac ttcgctttct atgcttttat ctttttatgg
     3901 ttatgaaaca actaaaaacc agttagctga gcaattggat tacgtccctg ttttcaatgc
     3961 agatgggact catggcgatc ctaatgaagg atttgttggt gatatcagtg gcggagactg
     4021 ggctatggtc gtctatgttc ccccagttgc ctcccttgct cagcaaatcg tccaaacgga
     4081 ttatcacact tttccaaaaa ccgatgctca actcgatgat atcaaaaaag cactatctaa
     4141 aggaaatcct gtctggactt ctgtgactat caattttgaa gtcccagatg aaactgattt
     4201 tatgacttgg accacaaata atggagaagt aagagtcact cctcttgttc atgcttgtgt
     4261 agtcactgga tatgacaaag aaaatattta tgtcaacgat ccctatggtg taaaaaatcg
     4321 agcagtaccg atcaacgatt tttcggcgat tttcactgca atgggtggac agatgctaac
     4381 tttggagaaa agctagtaat tcaaacaaaa atcccttata ctcaagatac caaacctttg
     4441 aagggagcgt agataatgga aaaattcaca cataaaaaaa tggatccaaa tgaaattccg
     4501 attatttttg tccgtgactg caagggaaat gttcaaggta aagtctctat taatgagtgg
     4561 aacgagcgtc gcagacctgc taccctcaat gaactggaaa tcaaattgta tcgtcaatct
     4621 cttgtttatt acgcagatca agagtacgag aaagccaccg atttattgaa atttctaatt
     4681 gctcgaacag agtatactcg ctttgaatat attgaacgtt tagcaaacat ctatcatatt
     4741 atgaatgaac ctgtcaaaga atatcagtta ttggacaccg tcttgtcagt agcagaactg
     4801 attgcccttc ctgctggatt agaaaagaaa ctagtacgcc gccttttacg agtaaagcaa
     4861 caactatccg atcaagaaaa ataacttgcg tttttttagg aaagtgctat gattattttg
     4921 ttgaataaat gggatgtagc caaactggta aggcacttga ctctgaatca agcaattgta
     4981 ggttcgaacc ctgccatccc aatttgatgt gcttgctgaa aaaatgaacc ccctgtaaaa
     5041 gaacaatctt tttgcagggg gattattgta ttatttttac tacttgtatt tagtttacat
     5101 aattaaatta tgaataataa aatcgggcgt attcttaata caaaaactga tttacccaat
     5161 aattgctaat gctttctccg gaactttttc tctaggaact tcctcatacg tttctatgtg
     5221 tgctccttta ttgctaattt tcaaataatg attttctttt aacacagaaa gaccattaaa
     5281 ttcaaccgtt cgttctttcc cattggcatc agcagctttc tgcacatacc ttgctgtacc
     5341 atattcattt acttcctctg gtttttgtgt ctttacatag acctctcctt tttcaactaa
     5401 tggatttagc tgatcaagta taccagggat ttctccataa cttccttcag tataatattt
     5461 cagtccaaat agcaaaatac cactaatcgc cgccagtcca atcaaatatt taatcacttt
     5521 catctttcta cctccttgtt atatattcag tatatgggtc agttaaagaa attccatccg
     5581 tcccaagttg gagatttttc tcctacttta gaagcgagaa tcgagtgttt ttttagtaaa
     5641 aatgatcgtt atttgatatt attgacgtgt ggataaatca gtttttctcg ttttgtccat
     5701 gtaaaaaatc atgttgatag caaaggatgt gttcaaaatg aatcaagaag aattatttca
     5761 aaaagtcaag gaaatgatta agaacggcaa tttcgatggc gcaaaacgat ttatcgaaga
     5821 acataaagaa caattaggac cttataaaga gaaagcccaa aatcttttaa aagatgtaaa
     5881 tatcgacagt gtcaaaaaca aatttaaaaa tctattcaaa taagtaaaac tttcactttt
     5941 gtaaaatcaa aggaggacaa tgctgctgca tcgtcctcct ttggttcttt tctgaatact
     6001 tcatactaaa ataaacgtgt cggatcgtaa tgataagtca cattgccgtt cagtgaggct
     6061 tttcccacca catatttacg attctccaac ttcagccacg cttctcgaaa agccattaat
     6121 tcttctcttt ttacatcaat cttttcgatt tttccatcga tcaaatcttg aataagttgt
     6181 tcatatggat tcattttttt cctccatctg ctgtcaatca ataagttgta aaaataggtt
     6241 catatgcttt tttggtatct agatcttctc tgactttgac tgtcgtataa ataatttttt
     6301 ctactgggat actataataa cttccttcac cattattgaa tcggtgaacg tctttctttg
     6361 ctaaaatacc atttatttta ttcgtcagta cacgttgttc gacactgtta cttacaacat
     6421 agtaaatcac ccgaatatta ttgatcaaat gcaaatcgat ttctaacact acatgatcca
     6481 tgaaaatccc actccttctt tcactttgat tatagcaagt ttctcctcta atttcaaaat
     6541 cgatacagat tttatcggat aaatccacaa aactgcttgc gccatttcgc acaaagtgtt
     6601 attttgtatc atggacttta tttatcggag gcaatagtat gatcgcgatc ggtcaatttg
     6661 ttttttatat tccttttttt attatgataa gcattctttt ctattacata aaatggacaa
     6721 aaaagaagtt ttcagtttta ctggcctctt tacctgctgt atattttact tatcaaattt
     6781 tttcttttag gcattgggaa acaacgtctg tattaataac acacatcatc gaattgacgc
     6841 tttcagtaat ttttcttatt atttggatct attttttata taagaatcag aattgaatcc
     6901 ctcgcatgtt taaaaagaaa acgattaaag atgcagcaaa tttctgccga tctttaatcg
     6961 ttttttctat tctgttatac caaaaaaatg tttgaatcgg tgaaggatcg ttccttgttt
     7021 taattctata aattcttgtg cgatcgggta cttttcttcc aaattttctt tgttcttttc
     7081 gatcaggctt ttcgcttctt catatttttt atctgcacat agtgctttta cttcctgtaa
     7141 tacttttcct gcttccattt gcctcaccct cttgccattt tattgcttta agttttattt
     7201 aagttatacg cattattcta gtttcatccc aaataacctt ttttcatttt tactcctcga
     7261 actaatcagt tttctaaagg ctgattggtt cgtctttttt atttttcctc catttcattc
     7321 tgctcttctg ttaatttatc taatgctttg attgatgctg cggctcttgc ataatcatag
     7381 ctgtataatt ccataaattt atcaggcaga acaaaccacg ctggatgata accgttagct
     7441 gtgacaggga atcctttatc ttgttggtta tctccatccc atctcagagc aacacctttt
     7501 tttcctttcc actttactaa agcgatcgag tatcccttct ttgtttgtgt gccgtcttca
     7561 ataattgcta aaatttctat tttttctttt ggcgcacgga cttcttgagg acgaaccatt
     7621 ttctattcct cccttatctc attacttcac gaccaacaca ctgcaagggg catgtgatac
     7681 gacataagaa gcggtcgatc caactaatgc ttgttgtata gcaccttttc ctgtcgctcc
     7741 cataacgatc aaatcaatat tttcttgtcc agggatgaca ttggcaatca aatttcttgg
     7801 atctccagtt tcaactattg cgtggatctc agcaatgccg tactctttgg catcattgat
     7861 ttttttcagc atggctactt ctgttctttg tttttcatct gcatagattt ttgaaaaggc
     7921 ataggcacta tgggtcagct caactttgtt gatgattgac acgataaata agtgggcttc
     7981 attgtccttt gctaagtcaa gggcttcagc gaacgctttg atcgattgtc ttgatccgtc
     8041 aactgctacc atgatgttct ggtatctttt actcattgga accgcctcct ttttctttta
     8101 ttgtaacatc tcaagcaaaa atacaaaaaa tatacgcaat atgtaaataa aatgatgaac
     8161 aaaagcaatt tctttcaagt tatttataca tttcttttct catcagaaca cctgttctaa
     8221 tcccattatt tctgcattat gtgaaattcc tcatcatttt tcacatcgag cgaacatcat
     8281 tctgttacaa aaaacg
//
LOCUS       DACKAC010000094         8284 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.096, whole genome shotgun sequence.
ACCESSION   DACKAC010000094 DACKAC010000000
VERSION     DACKAC010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8284
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.096"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            213..1406
                     /locus_tag="I0B46_002516"
     CDS             213..1406
                     /locus_tag="I0B46_002516"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAR2084763.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1536..3008
                     /locus_tag="I0B46_002517"
     CDS             1536..3008
                     /locus_tag="I0B46_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAR2084764.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3091..3879
                     /locus_tag="I0B46_002518"
     CDS             3091..3879
                     /locus_tag="I0B46_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAR2084765.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(3946..4614)
                     /locus_tag="I0B46_002519"
     CDS             complement(3946..4614)
                     /locus_tag="I0B46_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAR2084766.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            4729..6441
                     /locus_tag="I0B46_002520"
     CDS             4729..6441
                     /locus_tag="I0B46_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAR2084767.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6444..8216
                     /locus_tag="I0B46_002521"
     CDS             6444..8216
                     /locus_tag="I0B46_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAR2084768.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2537 a         1387 c         1821 g         2539 t
ORIGIN      
        1 aaaagcttag cagagttatc tttatggatg attgtgctaa gcttttttgg acagttctaa
       61 tgtaaaatat ttttatattt ttaagcaaaa aaatgaaaaa atacgagcag aaaactgttt
      121 tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg gagatgcctt
      181 gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta tttacatcag
      241 aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat gcaatcttgg
      301 atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct gtaacaactg
      361 gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt caaaaagtcg
      421 tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt gatggagata
      481 cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa ggagttgatg
      541 aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt ggtgctggtg
      601 accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg cctttaccaa
      661 tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca aatgaattaa
      721 cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat caaggacaac
      781 cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta gataatgaaa
      841 caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct gaattattag
      901 atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt ggtcctcaag
      961 gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt tatgctcgtc
     1021 atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct gccagctatg
     1081 ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa gcagaagtac
     1141 agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat actttcggta
     1201 ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc gatcttcggc
     1261 cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa acagctgctt
     1321 acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac aaagtagatg
     1381 cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc caagtggtgg
     1441 ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt atttatctat
     1501 ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca aatgttactt
     1561 tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt acaatcgtaa
     1621 caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg aactgggtat
     1681 tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa cttgcggata
     1741 aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc ggttcgttat
     1801 tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt caagggatgg
     1861 gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat tctattgaaa
     1921 agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt gtttttggac
     1981 cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt tttatcaatg
     2041 tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa ccaaaaatca
     2101 tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc atgcttgtct
     2161 cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt gtttctgctt
     2221 tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa agagctaaag
     2281 atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc aacttggctg
     2341 cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg tggatgcaag
     2401 gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta tcactagtct
     2461 ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact cattgggtct
     2521 tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt atgcctttca
     2581 gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc ttaggtgtga
     2641 ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag cttggtgtag
     2701 caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt tctatttttg
     2761 gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta acaaatgatg
     2821 cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat gcggatattt
     2881 tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta gttggcttgt
     2941 tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt ggtaaaataa
     3001 aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa agttattcaa
     3061 aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa tatttattcg
     3121 aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac aaaaaaggtg
     3181 attggcacca gttcagagca agagacaacg gatatacctg ttttatattt acttcatgga
     3241 atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta tgtagcaaac
     3301 tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga tacagtttat
     3361 gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca tgaattattc
     3421 ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat gggcggatat
     3481 ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat ctctctttca
     3541 ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa agcatctttt
     3601 tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga tccgcaatac
     3661 ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat ggcttgtggg
     3721 gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca ggaacaaggc
     3781 ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg ggaccaatgg
     3841 attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct taaaaagata
     3901 gctcctatag agatcacaaa catagtgatc tctataggag cttttttact gctcgttttc
     3961 tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg ttagaaagag
     4021 tgctttttca ttcaattctg catgggactg catactttct ttaaatccct ctaattgatt
     4081 ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat ttccgtccat
     4141 aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa tcaataaatt
     4201 gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac gaacaagtaa
     4261 ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag tattgatttc
     4321 taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca tatactgcag
     4381 caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt ccattgtttc
     4441 agccattttt tgcaagagct gttgtttgtt tttgaaatac caatataaag caggtgcttg
     4501 aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt gtgtttcagc
     4561 caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt ccattttatc
     4621 aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt ctaattttac
     4681 agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat gaaattaatc
     4741 ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc cgtagcagtc
     4801 atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga agctattatt
     4861 acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct ggctttgcta
     4921 ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca aggcgtaagt
     4981 gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg aaatatcgaa
     5041 cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca aatccaaaat
     5101 ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat tggtgccttc
     5161 attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct tatcgttacg
     5221 gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat gatcattcaa
     5281 aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat tcgtgtagtg
     5341 aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag tgaggactta
     5401 acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc cttcatgctg
     5461 gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt taaagatgat
     5521 cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat tatgatggca
     5581 atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc gctgaagcgg
     5641 attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc agaacaagag
     5701 ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga ggatacagat
     5761 acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat cgtcggggcg
     5821 actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga tccgacagaa
     5881 ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag tttaagacaa
     5941 acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc tcaaaacttg
     6001 cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc tattgctcaa
     6061 gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga agaacgtagc
     6121 agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt tatcggtaat
     6181 ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc tgaaaagctg
     6241 gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat cgcacaaaaa
     6301 attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag attagtcggc
     6361 gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat ctttgaaact
     6421 caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa tttttctatc
     6481 actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg atcattgcta
     6541 cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa ttagctaatt
     6601 acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct gtaatatgga
     6661 aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt attttgttca
     6721 cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac aaattggaaa
     6781 aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt cgttttacca
     6841 gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc atcacgaatg
     6901 ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag ttagcttggg
     6961 ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc cgcaaagcac
     7021 ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat atggatgaaa
     7081 aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg attgaaggat
     7141 tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct tattctggtc
     7201 tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt atctttttcg
     7261 gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc ttagttgtga
     7321 cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca tctggataca
     7381 gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt gatgaaaaag
     7441 atgaagtcaa accagaaaac ggtaaagcat ttgatggtgt tgaaaaaggt cttgcgttgc
     7501 agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc tcgatcaatg
     7561 tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa acgacgatca
     7621 tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc gatggaatag
     7681 atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt ttgcaagaat
     7741 ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg gacgcttcag
     7801 atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc atgacgttag
     7861 aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca ggtcaaaagc
     7921 agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt ttggatgaag
     7981 caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg gatgaagcaa
     8041 tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg aatgcagata
     8101 gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcat gagttattag
     8161 cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa agttaaatta
     8221 aaagaacctc gttataacgg ggttcttttt atatatttta tgttgtttgt ttttataagg
     8281 ggca
//
LOCUS       DACKAC010000095         8229 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.097, whole genome shotgun sequence.
ACCESSION   DACKAC010000095 DACKAC010000000
VERSION     DACKAC010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8229
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.097"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            328..1251
                     /gene="truB"
                     /locus_tag="I0B46_002522"
     CDS             328..1251
                     /gene="truB"
                     /locus_tag="I0B46_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAR2084769.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1255..2196
                     /gene="ribF"
                     /locus_tag="I0B46_002523"
     CDS             1255..2196
                     /gene="ribF"
                     /locus_tag="I0B46_002523"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAR2084770.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2591..3376
                     /locus_tag="I0B46_002524"
     CDS             2591..3376
                     /locus_tag="I0B46_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAR2084771.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3389..4240
                     /locus_tag="I0B46_002525"
     CDS             3389..4240
                     /locus_tag="I0B46_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAR2084772.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4364..4816)
                     /locus_tag="I0B46_002526"
     CDS             complement(4364..4816)
                     /locus_tag="I0B46_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAR2084773.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
     gene            complement(5281..5649)
                     /locus_tag="I0B46_002527"
     CDS             complement(5281..5649)
                     /locus_tag="I0B46_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAR2084774.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            5799..6281
                     /locus_tag="I0B46_002528"
     CDS             5799..6281
                     /locus_tag="I0B46_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAR2084775.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            6318..7328
                     /locus_tag="I0B46_002529"
     CDS             6318..7328
                     /locus_tag="I0B46_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAR2084776.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(7443..8156)
                     /locus_tag="I0B46_002530"
     CDS             complement(7443..8156)
                     /locus_tag="I0B46_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084777.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT         2784 a         1337 c         1667 g         2441 t
ORIGIN      
        1 ttaagaaact gaaaaaataa ataaaaaaaa acggaaaaaa gactggaaga atggacagtc
       61 ttttttttcg tttttgggaa gtgaaaacca aactatttac ctgtaagcca cccataaatc
      121 caatcaaatg gtgcaaagaa aaaatcttcc attctattcc ctcctttaaa aaaaacataa
      181 catgatttct atttacgtga gcatgaattt tgttacgttt ttattgccga aaaataaaaa
      241 ccgttcatta tgatgtcaaa ttcgtcagta attgatgcaa tgtgttataa taattcagtt
      301 aaatcaaaaa tgaaatggag aaaagacatg gacgggttat taactttatg gaaagaacgc
      361 ggaatgacaa gtcatgattg cgtattcaaa ttaagaaaaa tcttacatac gaaaaaaatc
      421 ggacatgggg gtacgcttga cccagacgta gacggtgtgt tgcccatttg tattggaaaa
      481 gccacaaaag ttatcgaata tttgacagat tctgggaaaa tctatgtagg cgagataact
      541 ctaggtttca gtacaacgac ggaagatcga tccggagaac gggtagaaac aaaaccagta
      601 gatatgtctt tatctgaaga aaaaatagat gaagtgatgg cttcatttat tggtgagatc
      661 acccagatac caccaatgta ctcagcggta aaagtcaacg gtaagcggtt atatgaatat
      721 gcccgcaata atgaaacagt ggagcgtcct gttagaaagg ctcagattta ccgttttatc
      781 cggacaagtg aattgacaag agacgaagtg acaggaacca tttcttggcg cttcgaagta
      841 gaatgcggga aaggaaccta tgtccgcaca ttagctgtag atacaggcaa gaaacttggt
      901 taccctgcgc acatgtctga tctgacccga acagcaagtg ccggtatgtc ccagaaagat
      961 gctctgacat tagaagaagt agctcaagct gtgaaaaatg ggacaatcga tgattgtttg
     1021 ctgccaatag aaaaaggagt gaaacgattc aaacgagtag atattactga tgaagtatgg
     1081 cagaaagtaa aaaacggtat gagacttaat tatactgtat ttgcgctccc agaaatgccg
     1141 tcagaagaaa ttgctttgtt ttataaaggc aaagttgtca gtatctatca gccaaacccg
     1201 aaagaaaaaa acaaattaaa acctagtaaa gtattgagaa atgaggaata acgaatgaat
     1261 atcatcaaga tcagacatcc ttaccaatca gaacaaattc cagaagacga tgtcgtttta
     1321 gtactaggat tttttgacgg cgtgcatatt ggacaccaga aagtcataaa gacaggtatg
     1381 gaaattgcac aaaaagaagg attgaaatta gcattgatga ctttcaatca gcatccatcg
     1441 attgtcttta aaaagatcga tcctagcagc gtgaaatatt tgactagcct taagcaaaaa
     1501 gaagaaaaaa tggccgcttt gggtatagat tatttatatg aagttgattt tacttcttct
     1561 tttgctcatt tagcaccgca ggaatttgtt gatcaataca tcgtagggtt acatgcaaaa
     1621 tatgctgttt ccggttttga ttatacgtat ggaccaaaag acattgcaga tgtcgctcat
     1681 tttcctgaat atgccaaagg aagattcaaa atcgtcacag tttctaaaga agaagaacaa
     1741 ggtgaaaaaa tcagctctac ccgtattcga ggattactag acagcggaca agtaaaagaa
     1801 gcagcaaaac tattaggttg cacatatgag gtagaagggg tagttgtcca tggcgatgca
     1861 cgaggcagac tactaggatt ccctacagca aatgtcaaag taaagagcac agttcatttg
     1921 cctaaagaag gtgtttatgt atcggaaata aaaattgggg aaatttggta tccagccatg
     1981 ggttcaattg gacataatga tacattcggt gaaggacggg agctgactgt agaattgtat
     2041 attttggact ttaatcaaga tatctatggt gaacatgtcg cagtacgctg gaatcaattt
     2101 ttaagagatc aagtgaaatt ttctagtgta gaggaattga ttgaacaact caaagaggat
     2161 gaacaagcaa cagctgaata tttttctcag caataaaaca ttatgcataa aatctcttgt
     2221 ttatgtataa ctagcaattt caataaaatg ggtgataaat aagcccagga aaggggctgt
     2281 gacaaaagtc ttgtcacagc ctttttttcc aaataaatgg tttttcagaa gtagcttctt
     2341 cggaaataag ccgaaatttc acaaaaattt gagaagtaat ttttgtaaat tacttcttat
     2401 ttcttgaagc tggccacttc tgtcacaacc tctttcttta tagaaataaa aggacactag
     2461 caattcatga aaacgattgc taaagcgtat catttgcttt agactatata tcttggtgta
     2521 cactcacttt aagacggaaa taaaagttat ccgtaataac aggtttaggt atgaaaaagg
     2581 agtgggtact atgtataagg aattagaaaa caaagtcgca gtagtaactg gtggctcaaa
     2641 ggggattgga acagcgattg ctcaacgatt cgggaaagaa aaaatgaatg tagttgtcaa
     2701 ctataactcg gataaagaag gagcagaaaa agcagtcaaa gcaatcgaag aggcaggcgg
     2761 aaaagcagtc tctgtacaag cagatgtcag ttctgaagaa ggaatacagc gtttacttga
     2821 tacggcttta gaaaattttg ggacactgga cgtttggatt aataatgctg ggatggaaaa
     2881 tcagcatcca acccatgaac ttagccttga agattgggaa agagtattaa aagtcaattt
     2941 gactggcgtt tttatcggaa cgaaagcggc tttaaattac tttacagaga atgataaaaa
     3001 aggaaatatc atcaatatgt cttctgttca tgaacaaatc ccatggccaa cgtttgctca
     3061 ttacgctgct tcaaaaggcg gagtaaaact attcaccgaa acggtagcaa tggagtacgc
     3121 accaaaggga atcagaatca atagtatagg acctggtgct atccgtacac ctattaatgc
     3181 ggcaaaattt gatgatcctg aacaaaaaga actcttggaa agtatgatcc cactaggacg
     3241 cgtaggtgaa ccaaaagaaa tagcggcgac agctgcatgg ttggcttcag acgaatccag
     3301 ttatgttaca ggtattacac tatttgcaga tggtgggatg actttatatc cttctttcca
     3361 aggtggaaaa ggataagggt gaataaaaat gggtttattg attgcattga tcccggcagt
     3421 tgggtggggg atccagccac tgattttgaa aaagattggt ggcagaccaa caaatgagat
     3481 attaggaact ggtattggag ccgtgctcgt tggcttactt gttcaattat tcgcttctcc
     3541 agctggtatt tcagtgacta catttttgat tagtttactt tctggtgcat tctgggtcat
     3601 cggacaaaca ggacagtata catctttcaa cttgattggt gtttctaaaa cgatgccaat
     3661 ttcgactgca ttacagttag tcggaacctc attgattggt gtcttcgctt ttggagaatg
     3721 gtctggaagt atgggaaaaa tcattggtgc agcagcaatc attcttcttg tatttggttc
     3781 tgctttaacg gcaatcagtg atgatcctgg aaaaaaaggc ggtatcacaa aaggaatcag
     3841 tctattagca tcaacaagta ttggttattg ggtttacagt gcattgccca aactagttga
     3901 tgcaagcgga ctttccattt tctttccgca aatgatcggt gtctttttag gggcagtgat
     3961 ttatgtgatt ttccgaaaac caaccgcatt ttctgaagac aagagttgga aagcaacaat
     4021 agttggagcc atcttcagtg tgtcagcatt ggcttatatt ttttcagcaa atgaaaatgg
     4081 ggttgctact gcatatatca ttacacagct aaacgtagtt atctctacat taggcggtat
     4141 gtacatcctg catgaaaata agacaccaaa agaaaaaaaa ttaacgatta tcggattatt
     4201 gctgatcgta gtaggaagca tggtcacggt ctttatttga ggataaagat ggtaaaatct
     4261 catttattaa aaaaatattt gtttgaacca aacagtaagt acttataaaa aaatagcgct
     4321 agaatttcca acatctctgg aatttctagc gctttttaat tgtttaagat acaagtgaaa
     4381 tatctgtctg catcaataat ttttcattga tccattgact aaaatttacg acatgattga
     4441 agttaccacc gattagtatc tccgcatgat tgtctttata aagatcgtat tgtaaaagac
     4501 ggtctttttt atttgcgtaa acatcgttat ggtactggac taattgttct cggatttgat
     4561 tgattgctgt aaaatcacga tactgaaggt tgaaacgcat cgtgattggt gcatttagca
     4621 tgaatcgctg catttctttt gctgcttcct ctgcttgatc tccgtatagg aactcaaaaa
     4681 gattacgttc agaaaatttc cattgcccgc caacctttgt tccttttagt tttccttcat
     4741 gaagataatt tctgatagaa cgtgtagtaa cacctaattg ttcggctaat tcatttactg
     4801 agtaaaaatc tttcaatgtt caccctccta tgtgaatggt tctactgact tagttgatat
     4861 ttgcttaatc acgaaatgta gaaagaacga ttttaattaa ggtcattgta aaaatcaagt
     4921 cagaaaattc taacttttct ttctctaaag aaattcgttc tctcctaaag tgatattata
     4981 tttaaatata cctgaaaata atgggcagta ttatgaaaga tcactgatat tttctcgtat
     5041 tttttcctta ttcaaaagtt ttgcaggaaa attgctgaaa aaataatttt actaagtgga
     5101 aaattcaaag ccattcaatc agtgattttt ttcagcacaa gaaagactcc ttataaaaat
     5161 agcagatact ttgtcaatta agtgtttaac cagggatgat aaaaaggata ggattgaaca
     5221 aaacctgtat aagctgatga atagtttcct cgccagtaaa aggtttattc gtataaagct
     5281 ttatcgccaa aatttatcat gacctttttt atcttcaata ttaaatgaaa tcaattgttc
     5341 aatcaattcc cagttgatta cacttttcca aggaactttg atgacgttat ccgtatatac
     5401 gtagcctgct tcttggatat ttttttcaag ggcacgaatc gctgcttttt ccggtgcgat
     5461 agaaaaatga tttttagcaa aactaaatgc aataataaag gtaccatggt cagtaaacat
     5521 aggttggttc cattttacag ttgtgtctag ctggggatac gtttctgtta tcttagcaaa
     5581 aaggtctttg atcttttgtt gatgttcttt ttctttgatc tgattgatga tcggctgaaa
     5641 tttttccatt ctaaaacgcc tccattaagt ggtttgattt attttaccag aaaaacaata
     5701 gttatgtata aaaaacgaac aaaacacaag taatgttgta accgctatat ttatatgata
     5761 agatgtctca aataataaaa tagaaaagag acataaaaat gattgacttg gcaaagaaat
     5821 tcttacacga aaatatatat tttgagttgt atcatcatcg agccatttat acaaatgaag
     5881 atgctttggt aataaagaaa gaacaaggat ttacaggtac agagaccaaa agcttattct
     5941 taaaagataa aaaagataat aactacgtat atctgacttt tactacgaaa caaacggatt
     6001 tcaaagcatt agggaagatc gtcggtaaaa ggctttctat tgtttcatca gaacgcatgg
     6061 aagaacaaac gggacaaaag cagggcgctg tttctccgtt tggttatgaa aaacagattc
     6121 cgattattat tgatgaagag ctcttatcaa atgaaaaact tgtttttgca ccaggtagac
     6181 cagatcaaac gatggtcatc caaacggttg acttaaacaa aatcatccag ctacttgaga
     6241 tgccgatatt tttgatgccg gcaaacgaaa aaaacaatta accaagtaaa atagtgcata
     6301 cttggaggtg aagcaacatg ataagttatc cattattaga aagaaatgca acgattgggg
     6361 ttactgcacc atcctcaggc gtatcagagg aacaccattc gttactcgaa tcagctttaa
     6421 agagaatgga agaaaaatct ttccaagtag ttactggaaa gacgctttgg acgcaagata
     6481 aggaaaaatc aggtccagca agaaaacgag cagatgaatt caatcaaatg atgcaagatg
     6541 aaaatatcca aatgatcttt ccgccttggg gaggagaatt attgattgag attctcgatt
     6601 ttgtcgattt tgatgctcta caaccaaaat ggatagtagg ttattcagat attagtacgt
     6661 tgctacttgc tgtcacatta aaaacaggga tagccactgc acatggaacc aaccttattg
     6721 atttaagagg agagtacttg gatgaaacga cagcaatgtg ggaaagtgta ctgaaaacaa
     6781 gagaaggaga atcgattcta cagcaacctt ctgaaaagta tcaaaaagag tggccccatg
     6841 agcattcatc atcttctgtt ttccatctca ctgaaccaac aaattggcaa tcagtcagcg
     6901 gagatgcagt aaggatgaag ggacgattat taggtggctg tattgatacg atcagtcatt
     6961 taatcggtac cccttatgga gatgtccaat cttttaaaag aaagtataat gatggaaagc
     7021 cgatcttgtg gtttctagaa aattgtgagc tatcgtcagt tgatttaaga agatcacttg
     7081 ttcagatgaa actggcagga tggtttgatg gcagctcggg gatcatgttc ggtagaagtg
     7141 cagcagagga tttgttagat tataaaatga aggatgtata tgaagatctg tcaaaggaat
     7201 taaaactacc gatcttgtat gatattgatt gtggtcatgt accgccacaa ctgacattga
     7261 tcaatggtgc ctatgcagaa gtagcggtag aagatagaaa agcgactgtt ttgcaaacgt
     7321 ttatttaaaa aattttttcc tgccttggag tttatcaaag accaacaaat ctaaatagaa
     7381 aagaaaatga aagaagctga cagtattttg ccagcttctt tttgtatgat ctattagaaa
     7441 ttttaattat ccataactat cacattgatc agctgtttca catcatttgg attacctgta
     7501 ggaatagcta ctacagatgc attatcaata atttcttgag ttgtttctct aatgaacgct
     7561 ggataagatg tttgatctgc aggcatttgc caatgtgaag gaactggata actagtgatt
     7621 gtcccgtcat ggttacttga atacactaaa actccctggt aactgtattt cccagtgagc
     7681 ataaccacat ctttcggata gactgcactc cctgcatcat aaggggcgat aggtgttcct
     7741 gcaggataat gaataacatg tagttcgaaa tcacccgatg tatctctaaa agtattacct
     7801 ctaattgcta gccaaacacg tgcgtactca atttgtagat cagaatatcc tgctaacgga
     7861 tctattgact gagaagactg tgaggaagtt gtgtctattg ttgaagaaga aatatttgtc
     7921 tcaactgtac tactagatgt agaagaagta ttgggctctg agactttact gcttgttgaa
     7981 gatgtattcg tttcagtaga agctgatttc tgagtacttg atttttcagc tgaactggtt
     8041 tgtttgattg aacttttgtc tgtggtcgca gatggatcac tattttcttt actaggagac
     8101 gtacatccta caaggaataa agagaccaac aataaaataa aacctaactt tttcatataa
     8161 aatacctcat tattttttta ataccagtat agcataatcc tgaatttttg aatccgtaaa
     8221 accttgtct
//
LOCUS       DACKAC010000096         7599 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.098, whole genome shotgun sequence.
ACCESSION   DACKAC010000096 DACKAC010000000
VERSION     DACKAC010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.098"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(137..427)
                     /locus_tag="I0B46_002531"
     CDS             complement(137..427)
                     /locus_tag="I0B46_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084778.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(545..805)
                     /locus_tag="I0B46_002532"
     CDS             complement(545..805)
                     /locus_tag="I0B46_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084779.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(942..1355)
                     /locus_tag="I0B46_002533"
     CDS             complement(942..1355)
                     /locus_tag="I0B46_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAR2084780.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1811..2296)
                     /locus_tag="I0B46_002534"
     CDS             complement(1811..2296)
                     /locus_tag="I0B46_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084781.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2435..2650)
                     /locus_tag="I0B46_002535"
     CDS             complement(2435..2650)
                     /locus_tag="I0B46_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAR2084782.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2874..3674
                     /locus_tag="I0B46_002536"
     CDS             2874..3674
                     /locus_tag="I0B46_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAR2084783.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3693..5561
                     /locus_tag="I0B46_002537"
     CDS             3693..5561
                     /locus_tag="I0B46_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAR2084784.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5554..6045
                     /locus_tag="I0B46_002538"
     CDS             5554..6045
                     /locus_tag="I0B46_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAR2084785.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6033..6587
                     /gene="srlA"
                     /locus_tag="I0B46_002539"
     CDS             6033..6587
                     /gene="srlA"
                     /locus_tag="I0B46_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAR2084786.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6605..>7599
                     /locus_tag="I0B46_002540"
     CDS             6605..>7599
                     /locus_tag="I0B46_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAR2084787.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2707 a         1213 c         1443 g         2236 t
ORIGIN      
        1 ttgttactat gaataacata ataagaaagt aaagtggatt gatttgagat ctttttatac
       61 ttcaaaaaag ataacaccca actttcaaaa atatgttttg gaagttggga caaacgagtt
      121 aatacatttt aatacattat tttttattca aaaaaaatag cacaatggca aagatcgtaa
      181 gagagatcat aagccccaat gaatgactaa aaagaaaagc agaatcacat gccatatcat
      241 cccatatttc ttcaagaaaa taaataagcg ggaaaaaggg aaggaatagt agaacatcga
      301 tccagcacca tttttttctt cttttgaaat aacataaaac aataaacatg aacgtgcagg
      361 gaagtaaaaa caaagcttta ctcataacta acaaaatacc agctcctttt atttttttct
      421 cccccacatt caaagagtaa caatggtatg tacttaataa tagaaaaatg gatcaacttg
      481 tatgaataac agctgaaaag atctgcacca aataaaaaac ttccagccaa aactgactgg
      541 aagtttattt ttttctgaaa tattctaaca caaatatgaa aaagatggcc gaagctaata
      601 agccgatttc gtagcaaaac aatgagatac cagagtcgta ataataaaat cctctaatct
      661 tatggataaa atagatcatc ggacaagcag ataagagtaa gtatttgtta ggccttttga
      721 ataagtaaaa gaaagcactg gtcattatga taaatggtat ccaaaacaaa agtttactta
      781 agacaatcaa atgatcaact cccatatttt ttaccaagag agatcataac atctttatcg
      841 taaaaacaaa atattttttt gctcattttg aaaatttaaa catactcgtt cttatccaaa
      901 tatataaaaa tcgcccctca ttcatcgaga ggcgataagt tctaatttaa ctcaattttt
      961 tttgtttctt ttggctgata agctgtttta ggtaatacca atttcagcac accgttttta
     1021 aaggttccag tgattttttc ttcatcaaca ttaggaagat agaatgaacg tttgtaagag
     1081 accgaatgtc tttctttccg tacatagttg ccatcttctt tatcttctgt atgggactca
     1141 tgattggcgg agatcgtcaa agtattatct gaatattcaa ctgtcgtgtc ttctttgtca
     1201 cagccaggca tatcagcagt tagtttgtat tctttttcat tttcgactaa atctactttt
     1261 ggataacttg aaacacctaa aaaatcattg aatgctggac taaacacgtc attgaaatca
     1321 gggaacatgt cgcgaatgct aggtacattt gccatttttg atcatccttt caataagtat
     1381 tttttattta tccctattta cgtaataact ctattttgaa aaaaatgcaa gtataatggt
     1441 tagctttgtg aatatttcat ctaatacgat acaaaggcta tctttctaat ttgaaaaagg
     1501 gatcattaaa ataataagaa gaaaatatgt caaaaggtaa aaaagtaaat tcttctatcc
     1561 aaatatagaa aaattgaatc tagaaaaccg cccgacaata aatttcatta actcagttga
     1621 ccgaaaatga agtagatacc aaaaatttat caagagagca agtagagaag tgagtatatt
     1681 cataagagaa atagctgtaa cgaattatta ggttaatgca aatgcttatt tatactccgt
     1741 ataactttct cagaaaaaat gataccaata atgacttgct agtactgtaa atccgtataa
     1801 tatcatctaa tcaaaatggt gttccatttg catctgtcat cgtaatcgat ccttcgggtg
     1861 acactcgata aaaaccaacg cttccagatc ctccttggga acggatttgt ttgctggaag
     1921 ctgtgaacaa ataatctcga tcaatttttt gataaaagct aataacaata tcgtcacttt
     1981 gtaattcgga acggtgggca ataagaaata gaagtgcttt gtcagagttt tctaatggaa
     2041 gagaaggtac agtgctttct tgcatttcag atgttgaaga aatgctaaac gactgaatac
     2101 ttgattggct atgttgcgaa tctacattac cagtagaagt attagtaatg gaagtagtag
     2161 aagtactttt agttgaacca acctcagttg ttgaaatggc tgctgaagat tctttaacag
     2221 aattctttgt tttatatcca caggcagaaa aaagtattac agttcccaac aagcctgcgt
     2281 aaatcccttt atgcatgata gctcctccta aaatagatta tctttattca gtatatcact
     2341 taaacaaaga aaagcagatg gtttaaaatg aaaaaacctt cccaataaag ggaaggttca
     2401 tgaagataat aactttttta ataattatag ctttctaata tctttcatca acttttctag
     2461 taatcattgg gaaaaatttc aagcagtaga tcgcacaaat acctacgacg atataaatga
     2521 tcttcgcaaa aatagttgtt gaaccaccag caatcatagc tactaaatca agttcgaaca
     2581 agcctaccaa cagccagttc aaccctccca caatcaataa agttaatgca atagcatcta
     2641 aagttttcat tagaattctc ctttcttaaa tgataaaaca attatatatc aaaaagagaa
     2701 acattgctta tataacgctc aggaatttta gcaatctatg caaaaagatg aaaatttttt
     2761 ttgctcttcc attagtacac attttttagc tgaagctgta aatttcatgt cttaattttt
     2821 tttagtgagc ttttatactg gatttgtaag aaaaaagaag gagaggatca catatgtcgg
     2881 attggttgga tattgcagga aaagtagtaa ttgtaactgg aggttcctca ggaattggaa
     2941 gaagtatcgt cgaaaatttg ttgaagcaga atgcacaagt agcgaatttt gatgtaactg
     3001 aatgccgtat acagcatgaa aacctactat ctttaaaagt cgatgtgtct tctaaaacgg
     3061 acatagaaga agggatatat aaagtaatga aacatttcaa gacgatcgat ggtctagtca
     3121 ataatgcagg aataaatatt ccttctcttc taatagatag aaatcatcca aaaagcaagt
     3181 atgaattatc agaacaagtt tttgataaga tgatcgctgt caatcaaaaa agtgtatatt
     3241 tgatgagcca agcagttgga agaatcttgg tgcaaaaggg atcaggagtg atcgtgaatc
     3301 tttcttctga atcaggattg gaaggatcag aaggacagag ctgttatgct gctacaaaag
     3361 cagcgatgaa cagttttact cgttcttggg caaaagagtt aggaaagagg aatgttcgtg
     3421 tggtaggtgt tgcaccaggg atactggaag aaaccggttt gagaacacaa gaatatgaag
     3481 aagctctttc ttatacgaga ggtataagcg tcgaacagct gagaaacggt tacagtcgaa
     3541 caagtacgat ccctctagga aggagtggta agctgcaaga agtggcggat ctagtctgct
     3601 attatctttc agaaagatca agttatatca ctggagtcac gactaatatt tcaggaggta
     3661 aaacaagagg ataaaggata ggggggacgc acgtgacgct agtaaacaga tggtaccaat
     3721 tattgaaatt actagtcact cacaagaaag tttcaatcca ggaagtgcaa aaagaaatca
     3781 aaaccagtaa tcaaaccata aaaaaagata ttggggagtt gaatgaagaa attgccggta
     3841 ttgcaaaaat catccaaaaa aacaaacatt ttgaacttga aatttatgat tttgattttt
     3901 ttgatgagat catgcaagga aaattgaaaa cagccagtga tttcaattcg gcatccaaaa
     3961 gaatcgctta tttactaaat aggctgatcg aaacagagga tttcttacgt atggatgatt
     4021 tgtctgaaga agtcggtgtc agcagaggaa cagtggtcaa ggatctaaag acattaaaag
     4081 agatgatcaa tgattttgat gtaaagatta caggcacacc aaataaagga ctaagaataa
     4141 taggagatga gctagaatta cgactacttt tattttattt tgtttctccc tacttttcag
     4201 agacgaaaac agaacagttt ctagaagaaa aacaagcaat caaacatttt cagaagaaga
     4261 cgaatgctag taaacaagaa atcacccttt taacgaaagt tatcggtatt tctttgaata
     4321 gaatctctag taactatctg ttgaagaagc ccatcaatcg ttacagtggg tgttttgaat
     4381 atatccaaga attcaatgaa ctgatcaatc agttggaaga aatctatgaa ctaactttga
     4441 gccaatatga aaaagatttt atcagttttc ctttgaacat catcaacaca agtataacga
     4501 tgataaggaa agctaactct gatttgcgct tatatttcga ccccatgatg atgcgtattc
     4561 gacgattaga tctcattgat ctggatgagt ccttcttata tcaagagatg aaacagcacc
     4621 tctctttatt gataaatcgg gtaatttttc gttatcgatt gactgattta ttttatggtg
     4681 agatagaaaa aaagtatcct ttttcttacc aaatcgctag cgaatgcata caagcattag
     4741 aagctatctt gcaacataag atctctaaag tagagatcag ctacctaacg ttgtatttgg
     4801 agttagcatt tcgatatcaa aaagagcatt tttcctctaa aaaagtagcc attgttcata
     4861 cgacaggaaa aggaacagca ttaatgatcc aaagacaaat caaaaggata ttggggaggg
     4921 atatacaaat aacacatgtt tcagaagaac aatatgaaaa agtagatcta aacaaatatt
     4981 tcgctgtttt tacaacgatt ccgttaaaaa atgtaaatga agagacaccg gtgatccatt
     5041 tgactaatct ttttgatgac tcttggttaa gggcacaatg gcaaaaagca aaaaaaaagc
     5101 gattagccgg gtttcattct actgtttttt tctatcaaat atggaaagaa aaaaagacgt
     5161 atcaagaata tttaaacaaa ttagtcgctc ctttgatcag aggctattat gtggatgaag
     5221 aatttgaaaa aaggatcttg aaaagagaat atcagcaaag cacatttgtt aacaaagaat
     5281 ttgcttttcc tcacacaacc aaccagctat ctcaacacat tgttttatca gtagggctaa
     5341 taactgatca aggtttagca gaaacaggag gaaatggagt tattttttta tttgccatac
     5401 ctgaagaaat gacggctgaa gttgagaatg aattatttga gctatacgat atcctatttg
     5461 aactcataaa aatgggaata gatgagttta ttgaattagc accgacacaa gaatcatttt
     5521 taaaactaat caaaagtaaa ggagagagac ttcatgaatg atatgtatct attcatagtt
     5581 ttcgcagctt cagcttatgt gctgcaagca gttatcagtt ggaagcaact aaagcatttc
     5641 aatcaaacat atagcgaact gcgaagaaaa ggaaaagtag cgattggtaa acgacctggg
     5701 aaaatccgtt caggcacgat tgttatgcta gctatagacg atcatgatca agttttagaa
     5761 gtcagaaaaa tgcaaggggt tacatctttg gcaaaatttc attcattacc tgtctatgcg
     5821 ggtgaagata tccattacat cgatcgttac catccctcag ttcgagcgga agatcgtttg
     5881 acacaagcag ctttagaaaa tgccagagaa gtatacttac gaatagaggc aggaaatgaa
     5941 aaaagtgaac aaccttcatt gccttttatt gctatgctca ataattttcg gatatggaaa
     6001 agtacgatca aagaaaaaag gagtgacaaa cgatggatat cataacaaaa tttgctgagg
     6061 gatttatgag gctgttccaa acaggtgcag aaacattcat aagctggatg acaggaatcg
     6121 taccagttgt cttgatgctg atggttgcaa tgaatacgct gattgctttt ttaggtgaag
     6181 accgagtaac gaaagtagcc aaattatcag cgaaaaatcc gcttactcgt taccttgtgt
     6241 taccgttctt atctgctttt atgttgggaa atcctatgtc atttacaatg gctcgtttcc
     6301 ttccagaata ctacaaacca agctattatg ctgctcaagc tcaattttgt catacgagca
     6361 atggtgtatt cccccatatt aatcctggag aattgtttgt atggatgggg attgctcaag
     6421 ggatcgaaac cttagggcta aattcgatgg agctggctat ccgatacttg cttgtgggac
     6481 tgctgatgaa ctttattggt ggttggataa ctgattttac tactggattt gtatgcagac
     6541 agcaaggaat cgttcttagc aaaaaagtag agctttcagt tgattagaga aaggagagtt
     6601 ttaaatgtat ggtgatatta agatcgtaag aggaaacggc gggtatggag gtcctttgat
     6661 tattaggccg acaaaagata aacacaagtt catctatgtc acaggtggag gtgagaggcc
     6721 aatgatcgta gataaaatag aagaactgac aggtatggag tctatcaatg ggttcaaaac
     6781 atcaatacct gatagtgaaa tcgcgcttgc aatcgttgac tgtggaggga ctcttagatg
     6841 tggaatttat cctaaaaaag ggataccaac catcaatatc gtttccacag gaaaaagtgg
     6901 cccattagca caatatatca ctgaagatat ttatgtttca gcagtagggt tagaacaaat
     6961 acagccacta atggaaactg atgataacaa agaaacagta aatactgcat ctccagataa
     7021 aaagtacaaa tatgatactt ctaaaaagat aaccgagcaa aaagcagagc agcaaaactg
     7081 gatagcaaaa attggaatgg gcgcgggaaa agtagttgct acattcaatc aatcagcaag
     7141 agaagcagta gatacgatga tccggacagt gatccctttt atggctttcg tttccctttt
     7201 gattggtgtg atacaaggtt ctgggatcgg taatgtattt gctaacatta tggcaccttt
     7261 agcaggaaat gcagttggat taacaatcat tggctttatt tgttcactgc catttttatc
     7321 tcctttgctt ggtccagggg cagttattag ccaagtgatt ggaacattga tcggtgtaga
     7381 gattgggaaa ggaaatattc ctccccaatt agcattaccg gcactatttg ctatcaatac
     7441 tcaaaatgca tgtgatttta ttccagtcgg tctaggtttg gaagaagcaa aagctgagac
     7501 agtagaagta ggtgtaccat cagttcttta ttcaagattt cttaacggca tcccacgagt
     7561 tatcattgct tgggtagcaa gttttggatt atatagctg
//
LOCUS       DACKAC010000097         7188 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.099, whole genome shotgun sequence.
ACCESSION   DACKAC010000097 DACKAC010000000
VERSION     DACKAC010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7188
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.099"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="I0B46_002541"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="I0B46_002541"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAR2084788.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="I0B46_002542"
     CDS             complement(1172..1867)
                     /locus_tag="I0B46_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAR2084789.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="I0B46_002543"
     CDS             complement(1880..2596)
                     /locus_tag="I0B46_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAR2084790.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSILYK"
     gene            2926..3534
                     /locus_tag="I0B46_002544"
     CDS             2926..3534
                     /locus_tag="I0B46_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAR2084791.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="I0B46_002545"
     CDS             3859..4182
                     /locus_tag="I0B46_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAR2084792.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="I0B46_002546"
     CDS             4364..5734
                     /locus_tag="I0B46_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAR2084793.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            6081..>7188
                     /locus_tag="I0B46_002547"
     CDS             6081..>7188
                     /locus_tag="I0B46_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAR2084794.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEESTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEGSPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKK"
BASE COUNT         2110 a         1445 c         1344 g         2289 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcgttatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcgaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttatata aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaacat actaagatta gaggggaaca tcattgacga atgatgttct cctctatttt
     5881 ctatttttag attatagtga agagtgaaag aaagttcgaa caaagccttg aggactacga
     5941 gtccctatca aaaaccgaac ttctttactg tcattttaga atcaggttta cgtatgttgg
     6001 ggccatgagc ccgtgtatag gaaattggaa tcgaaatgaa gcagtaaagt tctttcaaaa
     6061 cttaaaaagg aggaaaacaa atgccttaca ttcttgcatt agatgtttca atgggacaca
     6121 gctacagtgt cctatacaaa gacgacacct gcttatttga agacgagatt gaacacactc
     6181 aaagaggatt tcatgcatta ctcgaagaga tccagcactt gcctgagcgt ccattgattg
     6241 tttttgaagc aacggggatt tattccagac cagtcgaaaa gttctttcaa gatcatcact
     6301 tttcttactg tttgttaaac ccgttagagg cgaaaaaaca gatggaagaa agcaccttac
     6361 gcagttggaa aacagataaa tcggatgccc accggctcgc acagactcat cttaaaaatc
     6421 aacgtcagcc aaaagaagta caaagcaatt tttacttaga gatgcgcgat ttagcccgtt
     6481 tttatcaaga aattgagaag aaaatcactc gtctgcgcat ggatttacac aactgccttc
     6541 aattgacttt tccagagctt gaacagttct tttcaaacag gctgacccct tacgcattga
     6601 cattgatccg tctttttccc catccagatt ttgtattggc atctactcgg acaaaaataa
     6661 aaaataaact gatcaacgaa acacggaaaa aaatctccgc gaatcgtgcc gagcaaaagg
     6721 cagatcaaat catccactat gcacagtgtg cctatccagc tgttgaaaaa gacagtatcc
     6781 actgtcaaaa aacgatctat tacgccgaac ttctccaaga tttactcgaa caaaaaaaag
     6841 cactggcgac acaaatgatc aaaaaagccg aaggctctcc ttgctttttg ttgtaccaaa
     6901 cgttccctgg gattggcgca ctcacagccg ctttattgct cggtgaatta ggagatatca
     6961 cacgatttaa aacccataaa caattgaatg cctttgtcgg aatcgacatc cgacggtatc
     7021 attcaggtaa gtatactggc caagaccgca tcaataagcg tggtaatccc aaagcacgga
     7081 aaattatttt cttcaccatt cgaaatatga ttcgccagca acgcgcagct cctaatcata
     7141 ttgtcgatta ttactacaaa ttaaaaaagc aacctatccc taagaaag
//
LOCUS       DACKAC010000098         6681 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.100, whole genome shotgun sequence.
ACCESSION   DACKAC010000098 DACKAC010000000
VERSION     DACKAC010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6681
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.100"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..176
                     /locus_tag="I0B46_002548"
     CDS             <1..176
                     /locus_tag="I0B46_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="modulates transcription in response to the
                     NADH/NAD(+) redox state; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAR2084795.1"
                     /translation="YSQSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQT
                     LIYFDENYSEVFS"
     gene            569..3214
                     /locus_tag="I0B46_002549"
     CDS             569..3214
                     /locus_tag="I0B46_002549"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAR2084796.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            3524..4837
                     /locus_tag="I0B46_002550"
     CDS             3524..4837
                     /locus_tag="I0B46_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAR2084797.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            4827..5480
                     /locus_tag="I0B46_002551"
     CDS             4827..5480
                     /locus_tag="I0B46_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAR2084798.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            5535..6227
                     /locus_tag="I0B46_002552"
     CDS             5535..6227
                     /locus_tag="I0B46_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAR2084799.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6377..6577
                     /locus_tag="I0B46_002553"
     CDS             6377..6577
                     /locus_tag="I0B46_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAR2084800.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
BASE COUNT         2197 a         1120 c         1486 g         1878 t
ORIGIN      
        1 aatattcaca atctgctatt gatgaaattg tcaaaggaaa tgtaaccgct atacttaatt
       61 ttgcgcctga cagagtaact gtcccagctt atgtcaatgt tcaatacatt gatttgacaa
      121 ccgagttgca gacgcttatt tactttgatg agaattactc agaagttttt tcttaggaaa
      181 atttgagtag gcttattgat cttgcgagaa ctcgtcgtaa ttctttcaaa aattttttag
      241 tactaaatga agtggttttt tcacgaccac ttgttgacta aaaaaaaaaa ccattgtaga
      301 atgaagcata acgctgaaca agaggagtaa ctggcacatt cattcttaga gagagaacta
      361 tctgctgaaa ggttctcaat gacaaccagt gaaggtagct tgggagtttc tgtcttgaat
      421 ggagtagaga tagacgagtc gtgatcgtta actcacttga gaggcaataa tgattgatta
      481 ttgcaattta ggtggtaccg cgtaaatcat acgtcctaga atacgaaagt attttaggac
      541 gttttttagt ttaaaggagg aaaacagcat gacggaagaa aataacctat caacaaaata
      601 taatccgcaa gaagttgaag ctggacgtta tcagcattgg ttagaacaag atttgtttaa
      661 accgagcgga gataaaaaag caaaacctta ttcaatcgta atcccgcccc caaatgttac
      721 tggaaaactt catttagggc atgcttggga tacgaccttg caagatatgc tgattcgtca
      781 aaagagaatg caaggatatg atactttatg gctgcctggt atggaccatg caggaatcgc
      841 gacacaagca aaagtagaag cgaagcttag agagcaaggg atttctcgtt atgatctagg
      901 tcgtgaaaag ttcatcgatc aagtgtggga atggaaagaa gagtatgcga gccacatccg
      961 cgaacagtgg gctaaaatgg gcttatcttt agattacagt agggaacgtt ttacattaga
     1021 tgatggatta tctgaagcag tacgtaaagt ttttgttacc ttatacgaaa aaggtttgat
     1081 ttatcgtggt gaatatatca tcaactggga tcctcaagca cgaacagctc tatctgatat
     1141 cgaagttatt cataaagata tcgaaggcgc cttttatcat atgagctatg aactagcaga
     1201 tggatcaggc gtagtagaaa ttgcgacaac ccgtccagaa acgatgttgg gagatacggc
     1261 gattgctgtt catccagaag acgagcgtta tcaagcgctc atcggcaaaa aagtgattct
     1321 acctttagtc aataaacaaa tcccaatcat tgcagatgaa tatgtagata tggaattcgg
     1381 gacaggtgta gtaaaaatca ctccagcaca tgatccaaac gactttgagg taggtaatcg
     1441 tcatgatctt ccacgcgtaa atgttatgga tgatgatggt acgatgaatg aactagcagg
     1501 aaaatatgct ggaatggaac gttttgctgc tcgtaaagca atcgtgcaag atctgaaaga
     1561 aatcggtcga ttgattaaaa tcgatacgat gatccacagt gtgggtcatt ccgagcggac
     1621 aggtgtcgtt gttgaacctc ggctgtctac tcaatggttt gtaaaaatgg cgccgttagc
     1681 gaaaaaagcc attgataacc aagaaacaga tcaagcagtg gaattcttcc ctccaagatt
     1741 caatcaaacg tttttacgtt ggatggaaaa tgtccatgat tgggtaatct cacgacaatt
     1801 atggtgggga catcaaattc ctgcatggta tcacaaagaa acaggtgaaa tgtatgtagg
     1861 gatggaagca ccagcggata gtgaaaactg ggtacaagat cctgatgttt tagacacttg
     1921 gtttagttca gctttgtggc ctttctcaac aatgggctgg ccagatacgg aaagcgaaga
     1981 ttataagcga tacttcccta ctagcacttt agtaaccggt tatgacatca ttttcttctg
     2041 ggtaagcaga atgattttcc aaagcttgga atttactggc gaacgtccat ttgagaatgt
     2101 cttgattcat ggattgattc gtgacgaaca agggcggaag atgagtaaat cattaggtaa
     2161 tggaatcgat ccgatggaag taatcgaaaa gtatggagca gatgctctcc gctggttctt
     2221 atcgaatggt tctgcaccag gacaagatgt ccgcttcagc tatgaaaaaa tggatgcttc
     2281 ttggaacttt atcaacaaaa tctggaatgc ttcacgtttc gttatcatga atatcgaagg
     2341 aatgaccgta gaagagatcg atttatctgg agaaaaatca gtggctgacc ggtggatcct
     2401 tactcgtttg aatgaaacga tcgaacgagt aactgagcta tttgatcgtt ttgaatttgg
     2461 tgaagcaggt cgacaactgt ataactttat ctgggatgat ttttgtgact ggtacatcga
     2521 aatgagtaaa gaagtacttt atggagatga tgctgtttca aaacaaacca cccgtagcat
     2581 cttagtccat acacttgatc agatcctacg tttattgcat ccaatcatgc cattcgttac
     2641 agaagaaata tgggaacata ttcctcatca gggaaattct ctagtagtgg cagaatatcc
     2701 tgttgtacgt ccagagtttg atgatgaaac agcttctaaa gggatggaag tattgaaaga
     2761 attgattcga gccgttcgaa atattcgttc agaagtgaat acaccattgt ctaaaccaat
     2821 tacgttgcta attaaaacaa acgatgctaa aatcgatcaa tttttagttg aaaatacaag
     2881 ctatatcgaa cgcttctgta atcccgaaga gttgacgata tcaagtgaga tcactgcgcc
     2941 agatctagcg atgtcagctg ttctgacagg tgcagaaatc ttcttgccgt tagcaggatt
     3001 gatcaatatt gaagaagaaa tcaaacgatt agaaaaagaa ctggaaaagt ggacaagcga
     3061 agtaaaacgt gtccaaggaa aactttctaa tgaacgtttt gtttctaacg cgccggaaga
     3121 agtagtagag gcagagcgtg caaaagaaaa agattattta gagaagcaaa cggctgtcaa
     3181 agaacgaatc gaaagtttgc gtactattca ataagaatag aaaaagagag tgtgaaatca
     3241 gctagctatt actaaaagct agctgattga acatcgttac aggaaagagt ttgagcctca
     3301 ctcttagctt cgattcataa ggagatgtga cataaatttt gtcgcatctc ttttttagtc
     3361 tactcttatt atgtaggaga aggagggagg tggatcatgt atcaagagtc aataaagtag
     3421 ttgataaaat cgttcctctc tctgtttctt ttctctttgt catactttta tttttcttat
     3481 gtgttctatt tgttataata aaagagaaat gggggaattg ttgatgacga ttgaagaagc
     3541 aatagaatgg atccatagtc gattaccatt tggttcacgt cctggccttg accgagtcga
     3601 agctttatta gaagtggtag ggcatccaga gagaaaagta aaaacgatcc atatcgctgg
     3661 aacaaacgga aaaggttcta ctgtagctta tttgagaagc ctattagaag aggctgggct
     3721 gacagtaggt acatttactt caccttatat cgaaacgttc aatgaacgga tcgcaatcaa
     3781 tggcaaaagc attccagaca atgatttgat ccagcttgta aaaaaatatc agccactgat
     3841 caaacaactt gatcaatttg aagcagtagc tggtattact gaattcgaga cattaacagc
     3901 aatggcattt gattattttt tgatgaagaa agtggacgta gcgattatcg aagttggctt
     3961 aggcggctta ttggattcta ccaatgtagc tgtaccaatg ctgacgggaa taacaacgat
     4021 tggacttgat cataccgaaa tacttggaga tacaattgaa gaaatcgctg cacaaaaagc
     4081 tggaatcatc aaacaaggcg ttccagtagt aacaggaaat attgccccag aagcttttac
     4141 tgtcatccaa caaacagcta aagcaaaaaa tgcaccgctt tatgtatacg gggaaaatta
     4201 taaaataaac tatctgcatc ctgatgagat gtggggagaa gtatttgaat tttttaatga
     4261 aataggaaag atccctcaat taaccactcc gcttttaggg caacatcaag ctgaaaatgc
     4321 aggagtagcc atcgaattgt attttgttta ctgtcaattg gaaaagctgc ctttccgaga
     4381 aaaagatgtt ttgaagggat tgaagaaatc tcgatggcca ggaagaatgg agcgtttgag
     4441 taacgagcca ttagttgttt tagatggtgc ccataatacg catgcaatga accggcttgt
     4501 tgagaatttg aaaaaagaat tcaaaggata tcaaatcaac attttgtttt ccgccttacg
     4561 aactaaaaat gttagagaaa tgctagaacg attgcagcaa gtaccaaatg tccatattta
     4621 tttgacgacc tttgaacatc caaaagccat cgaattaagt gaagtatcag atttagcaag
     4681 tgagagagta tcaatcgttt cattgtggca attcggatta gcagatatat tggaaaagat
     4741 gacaaatgaa gacgtattag ttgtgaccgg ttcactttat ttcatttcag aagttcgcca
     4801 tttattaata caattaggag gagatcatga agtataatgc agtgattttt gacatggatg
     4861 gtttgctgtt cgacacagag ttagtgtatt acgaagccac acaagtagta gcagacaaga
     4921 tgggttttcc ttatgataaa gacctgtatt taaaattctt aggtgtttca gatgaagaag
     4981 tatgggcgaa ttatcatatg attttctcgg aatttggcaa aacaagtgta caaagattca
     5041 tagatgattc ataccaggaa acgcttgaac gtttttcgca aggagcggta caaatgaagc
     5101 ccggtgtgtt ggaactcctc tcttttttag aagaacatca gattcccaaa gttgtcgctt
     5161 ccagtaacca acgagcagtc attgaacttt tattgaagaa gaacaatctg tttgaacgat
     5221 ttgagacgat tgtttctgca gaaaatgttc aacgagcaaa accagaccca gaaattttct
     5281 tgttagctaa tcagaagcta ggtacagcga agaaagaaac attgatttta gaagattcac
     5341 aaaatggtat tttagcagct tctgcggctg agatcccggt agtcatggta ccagatctgc
     5401 ttccaccatc ccaagaattg atcaaaaaaa cagctggggt tgtttcttct ttacatgaaa
     5461 tccctgcatt gttaaaataa aaatccatct cttttcgtat cttattttag ataagataga
     5521 aaggagatgt ttttttggga aaacgattga tcaaagaagt acctactagc tctttaccaa
     5581 gggaacgtat ggaaatttat ggcgcggaag ctttatcgga tcaggagctt ttggcaatct
     5641 tactgcgtac cggtcaacac ccttatagtg tgatgtcgat tgctggaaat ctgttaaaaa
     5701 cgtttggagg actggcctct ttacgacagg ctactttgca tgagttagaa gagattcgag
     5761 ggataggacg cgtgaaagca atcgaaatca aagccttgat cgaattaggt cgacgaattc
     5821 agacggatca tgaaagagcg gctccaccgg ttcgtaccag ctacgaattg gcccagcaat
     5881 tgattctgga aatgaaagat cacaaacaag aacatttggt ttgtatttat ttggacacga
     5941 agaatcaagt gatcttaaaa aaaacagtgt tcatcggttc gctgaatcag tcagtcgctc
     6001 atcctagaga aattttccat tatgctgtac gttattgtgc agcaaggatc gttttagcgc
     6061 ataatcatcc ttctggtaat gttattccat cgcagcagga tatgaatttt accaaaagga
     6121 tacaaaagtg tggagaaatg atggggatca ctgtactgga ccacttgatc atcggacgca
     6181 aacgatattt tagtttgaga gaagaaggaa tgatggaaga gaagtaagtt gctttcttgc
     6241 aaaaaaaatg aagcaagtgt acaattatat tgtaattttg tttgggaata gaagttgaaa
     6301 catctgttgc acctctatcg tacacgagca atgttacaat ttaaattagt gcttttggca
     6361 cgaggaggat attattatgg aaaacggaac agtaaaatgg ttcaacgctg aaaaaggatt
     6421 tggatttatc tcacgtgaag atggaagcga cgtattcgtt cacttctcag ctatccaagg
     6481 tgacggattc aaaacattag aagaaggtca agcagtgact ttcgacgttg aagaatcaga
     6541 ccgcggccca caagctgcaa acgttgtaaa agcttaattt tatctgtaaa tcaaattgat
     6601 ggtagacatc aatttttacg agagaacaaa gcgagtaaca aaaatacctg ccacaagaga
     6661 caatttctct tgtggcaggt a
//
LOCUS       DACKAC010000099         6644 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.101, whole genome shotgun sequence.
ACCESSION   DACKAC010000099 DACKAC010000000
VERSION     DACKAC010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.101"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(1..859)
                     /locus_tag="I0B46_002554"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="I0B46_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="I0B46_002555"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="I0B46_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAR2084801.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="I0B46_002556"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="I0B46_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="I0B46_002557"
     CDS             complement(2143..2373)
                     /locus_tag="I0B46_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAR2084802.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="I0B46_002558"
     CDS             complement(2603..3421)
                     /locus_tag="I0B46_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAR2084803.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="I0B46_002559"
     CDS             3582..4271
                     /locus_tag="I0B46_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAR2084804.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="I0B46_002560"
     CDS             4285..5787
                     /locus_tag="I0B46_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAR2084805.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="I0B46_002561"
     CDS             5800..6276
                     /locus_tag="I0B46_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAR2084806.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="I0B46_002562"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="I0B46_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2172 a         1032 c         1204 g         2236 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttgttatcca aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atgt
//
LOCUS       DACKAC010000100         6289 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.102, whole genome shotgun sequence.
ACCESSION   DACKAC010000100 DACKAC010000000
VERSION     DACKAC010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.102"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            132..1106
                     /locus_tag="I0B46_002563"
     CDS             132..1106
                     /locus_tag="I0B46_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAR2084807.1"
                     /translation="MRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAGQYLFD
                     KELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGAMTICL
                     SNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWETCLNEF
                     DKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFMEMQWIN
                     SNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVLDQADF
                     DLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            1088..1549
                     /locus_tag="I0B46_002564"
     CDS             1088..1549
                     /locus_tag="I0B46_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAR2084808.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1599..2063
                     /locus_tag="I0B46_002565"
     CDS             1599..2063
                     /locus_tag="I0B46_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAR2084809.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            2077..2826
                     /locus_tag="I0B46_002566"
     CDS             2077..2826
                     /locus_tag="I0B46_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAR2084810.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2826..3656
                     /locus_tag="I0B46_002567"
     CDS             2826..3656
                     /locus_tag="I0B46_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAR2084811.1"
                     /translation="MENSTEKKRLITKKDLHKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTPFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3656..4303
                     /locus_tag="I0B46_002568"
     CDS             3656..4303
                     /locus_tag="I0B46_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAR2084812.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKINYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(4401..5177)
                     /locus_tag="I0B46_002569"
     CDS             complement(4401..5177)
                     /locus_tag="I0B46_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAR2084813.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLTESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            5259..5402
                     /locus_tag="I0B46_002570"
                     /pseudo
     CDS             5259..5402
                     /locus_tag="I0B46_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608918.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
     gene            complement(5519..6289)
                     /locus_tag="I0B46_002571"
                     /pseudo
     CDS             complement(5519..6289)
                     /locus_tag="I0B46_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT         2112 a          887 c         1120 g         2170 t
ORIGIN      
        1 attttattat ataaaattgt tataaaaaat ttttttgaaa aatagtattg caaaataaag
       61 caatttcata gataataggt atcgtaaagt tatataactt tataatacct attagagtga
      121 gggataaaga aatgagacaa ctacacaaag aacagattga aacagcaatg gcagcgttaa
      181 aggaaaagac aattgatcat gtatatttta cagcatgtgg gggttcacaa gccgttttaa
      241 tggctggtca atatctattt gataaagaat taactgtacc ttgtcatgtt tatactgcta
      301 acgaatttaa ctatgctata ccaaaaggat tatctgaaaa ttctttagtt atcacttgtt
      361 cccattctgg cacaacacca gaaactgtag aagcaacaaa aaaagcaaga gaatttggag
      421 caatgactat ttgtttatct aatgaagaag gatctcctct atgggaagca gctgaatatc
      481 ctgttcacta tgattggggt aaagatgttg atgctagtga tttaaataaa ggaattcttt
      541 atggtttatt atttaatatt ttacttgttt tagatggctc taaaaaatgg gaaacttgct
      601 tgaatgaatt tgataagtta aatgatcttg ttattgctga aaagagcaaa tttgaaataa
      661 ctgctaaaga atggggtaaa aaccaaaaac gagataaaat tatttacaca attggtagcg
      721 gtattaatta cggagaaatt tactccacag cagcttgctg gttcatggaa atgcaatgga
      781 ttaattctaa tgctattcat tctggggaat ttttccatgg tccttttgaa atcactgatt
      841 atgatgtacc attcatctta gtgaaaagta ttggagatac aagattttta gatcaacggg
      901 tagaagactt cactaaaaaa ttcagtaata aattaatggt tttagaccaa gctgattttg
      961 acttagaatc tatagatagt gtagcgaaag aatatattgc agctgtactt agtggcgttg
     1021 ttattcgcca aatggtagaa gcgattgctt ttgaaagagg ccattcctta gatgttagac
     1081 gctatatgtg gcaaatgaaa tattaattaa taggaggttg caaaagatgg caaatcaatt
     1141 atttcacatt gtgattacaa cacattctac cctttgtgag ggttatttaa aggcaacaaa
     1201 attaatttta atgaatgacg ccacaggtat tagtgctgtt ccatttaaag aaaatactag
     1261 tccagatgat tttgaggaga gtatgaaaga aataattagt cagcatcatt tgcaacctct
     1321 aattatttta acggatttaa ttggaggaac acctaataat gtttctatta aacagttgaa
     1381 caacaataga attcaaatta ttgctgggat taatttacct cttttactgg agttattaat
     1441 ggcccagtca gctggtaaat ctatagaaga aatagatttt aaggaattac tgaataactc
     1501 tcaaaaaagt atggttcata ttaattcgct tttaaaagat gtcagttaag gtggcaattt
     1561 ctattctata aattttaaaa attgaaggag gaaatagaat gattaaatta gtgcgtgtag
     1621 accacagatt acttcatgga caagtaatat tttcttggac taaacagtat gatattaacc
     1681 atattattgt ggctgataac aaagttccag gagatccaat gtctgtgatg gctttgacaa
     1741 ttgctaaacc tgctgattgc aaattagaca ttgttaaaat tagtgatgtt aaagggatta
     1801 tggaaacatc cccaaatgat aattacatga ttttaattaa aggacctaag gaagcttttg
     1861 aattagttca aaatttacca gaaattaaag aaattaattt tggcggtgtg tctaagaaaa
     1921 gcgagtccaa acaatatgga aaggcagtct ttttaactcc tgaagaatta gaagccacaa
     1981 aaaaaattct tgatcaagaa gtgaaaatat ttgtgcaaca agtcccatct tcaagtattg
     2041 aaaatgtaga ttttagtaag taagggggga aaataaatgt tattaaaagc cattttatta
     2101 ggtttagttg gtgtggtagc tgttattgac tccaggttaa ttggtcgaca aaatattggt
     2161 agaccactaa ttttgagtac acttgccgga cttgtgctag gtgacttgca tacagggatt
     2221 gttcttgggg catctttaga attaatttct atgggattcg tctcaattgg ggcggctgga
     2281 cctcctaata tgcaacttgg tagtattatt gccactgctt ttgcaatttt aactaatagt
     2341 agtacagaaa cagccttaac aatagctatc ccagtggctg tagctggtga atttatgagt
     2401 attatcatgc gcatgataat tgctcaattt ggacatgctg cagataaagc cattgaaaat
     2461 aataaattta gaaaagctca aattattcat atttattggt catttatttt taatgctttt
     2521 gtttacttta ttccaatctt tttaacagta tattttggtg ctgacgttgt ggcagattta
     2581 gtagataaaa ttccagaaat gattacagac gccttgactg tctctggaaa tatgctttcc
     2641 gcattagggt ttgcaatgct attaagcaca atgttgagta aaaaattata tccttacttt
     2701 atttttggat tctttattgt tgcctattca ggtctttcac taattggcgt gacaattttt
     2761 gcagctctga ttgcctttgt aatggatcaa gttaaatatg gaaaaagaga agaggcgcat
     2821 ggttaatgga aaatagtaca gaaaaaaaac gtttaatcac aaaaaaagat cttcataaga
     2881 tgtgtcttcg ttcatggaca atcatgtgtt cgtggaatta tgaaagacaa atgcatatgg
     2941 gatttatgta tgggatggcg ccaattttaa ataaagttta taaaaatgat gaagagaaaa
     3001 aaagagaagc ttatactcgt catatggcat tttttaactg tacgcctcaa atgactccat
     3061 ttattatggg actttgtgct tctatggaag aacaaaatgc tcaagcagaa gaaggaaact
     3121 ttaatactga atctatctcc atgattaaaa ccagtttaat gggtcctttt gctggaattg
     3181 gtgattcatt tttccaaggt actttaagaa ttattacatt tgggattgga ttgtcttttg
     3241 ctaagcaagg tagtatttta ggaccgattt tagctgttgg cctttttgct ataccttcta
     3301 ttttagctgt tttttacggc ggtaaattag gatatgagtc tggcaataaa taccttacta
     3361 aattgtatca agaaggtctt atggatcgag taatgcaatt tgcttctatc attggtttgg
     3421 ctgtagtagg tggaatggtg gctagcatgg tgccaattgt caccccttta aaattcacta
     3481 cagggggaac agaacttgtt ttacaagact tacttgattc cattattcct ggattattac
     3541 cacttgtatg tactttggga atatataaat tgattaagaa aaatgttagt acaaatatgc
     3601 tcttattagg ttgtattttg gcagggtttg ttttaagtat aataggtatc ttataatgat
     3661 taaggctcta atttttgata tggacggagt tttaattgat agcgagatta tgtatcaaaa
     3721 gtgggtattt aattttttaa aacaagaaaa tttaagctat cctttgtatt cttatcaaaa
     3781 aaagattgga actacagcaa gtatttttga tgatatagaa aagtacaatg agggatgtaa
     3841 cagtttagaa ttgaagggaa aatttactaa atattggaaa tcgcaaaaga ttaattactt
     3901 ggatattttt cctaatatac taattgaaca gcttgaatgg ttaaaaaaga aaaaaataaa
     3961 aatggcaatt gcttcttcca gtccaatgga tactataaat gaagttgtta ccacttgtaa
     4021 aatagagtca tattttgatt gtttaattag cggaagagat ttgccagaaa gtaaacctaa
     4081 tcctactata ttccttaaag cttcagaaca gctcagaatt ccagttgaag aatgtctggt
     4141 gatagaagat tcttataatg gtataaaagc tggtaaaaga gcaaatatga aggttcttgc
     4201 aattaaagat aaacgttttt ctcaagatgt aagtctagca gatgatgtta tttacgatat
     4261 taaatatctg cgacaagaag tacaggtgaa atttggaata taaataagtt ttttgatgta
     4321 tttagagaat attagtaaaa ataaaaatca tgcccaaatc taatcaaaat tgattgcttt
     4381 gggtatgatt tcttttaata tcaatctatt actttaggat ctgaatcatt tttatgcacc
     4441 gtatctaaag tgatattttc tgtgacttca ataacataac taattctatc acaacgaact
     4501 gtggattgag aataatgaaa tggttgatta tttttatctt tgccaacctt ttctaaaata
     4561 aatagcggat ctcctactat acattgcaat aagtcggaaa gctcaggtgt tgcagataaa
     4621 ccgtttattt ccataataga gctcgtagct ttgacatcat agtcatcttc tagaagttta
     4681 tataatgaag tatattggct acctttttca aataaagttg gaaatttttc ttctgatgcg
     4741 aaaaaattat caattcccat aggggcaccg tcttcccaca ttaaacgttt aatattgtaa
     4801 actctatcag ttacattgat ttgtaattta ttagctaaaa actcatcagc ctttataatt
     4861 ttagaatgta aaacagtata tctaatttta tgtcctcggg tagaaagaga atcgctaaat
     4921 ccatgaactc cagctaattt taaactttct tttttttgag ttacaaaggt ccctttcccc
     4981 cttaatcgtt caactgttcc actttctgtt aaaagtttaa aagcttttcg aatggtaatc
     5041 ttacttactc cataaatttt acataattct tcttctgtag gtaattgctc tcctggaaga
     5101 tatttttttt cacgaatatc gttacgtata gaagcttcta cttgtagata taatggtcta
     5161 ttatcttgaa aattcatagt acactcccta aaaagttata cttcattata tcacaattta
     5221 ttaaaataaa agattaagat ataaatataa atgaactagt ctttgactaa ccaatgcccg
     5281 cttttgaaag aaaacaaatt cattctgttt taagtaaaaa tccttatgtt accactcatt
     5341 ctgaagggga tgagccttat cgctacttgg ttgtggaacc aagtgataag acaattttat
     5401 aaattcaata ttaattaaaa taaaaacaaa caaagtagat tattttactt tgtttgtttt
     5461 tttattggga gcgtcaataa ttttgtgtaa ataactcgtc cttctgcaaa ataatgggtt
     5521 actcagtaaa cattgaagct aatgtatccg tcacttgttg gaagccttta tggcttctat
     5581 ttaagaattt ttgattgtat gtatcaaaga tggatactag gaaacgctct agtgattctt
     5641 cattttgaaa ctgctctttt ctgcggctgt atttcttgat ttgtttcttg aaagattcga
     5701 ttaaattagt tgagtaaatg cttcggcgga taccaggcgg aaactcataa aaagtcaata
     5761 aatcttgatt ttttatcagt gactgcgtca ctttaggata agttttttgc tacttctcaa
     5821 tcatactccc taaaaaggta tttgcctctt cctttgagct agcttgataa acagccttaa
     5881 aatcatcata gatttctttt cgatctttga cacgtacttt atgagcaata ttacgggaaa
     5941 cgtgaacaca gcaatgttgg tatttagctt taggataaat ctggtgaatc gtatctttca
     6001 tgccttttaa gccatcggta ataaacagta agacatcatg aacccctctg gagtaaatgt
     6061 cctgtaacaa ctcattccaa acgtaggtcg attcagtcgg agcaatcgca taacttagta
     6121 cttctttagt gccgtcttct cgtataccaa tggcaatata gattgcttct ttggagacgg
     6181 tttgccgttt taaaggaata taagtagcat ccatgaaaat agcgacatac ttatcattta
     6241 gagttctaac tttaaatgca tttacttctt cagtcagcac tttagtcat
//
LOCUS       DACKAC010000101         6255 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.103, whole genome shotgun sequence.
ACCESSION   DACKAC010000101 DACKAC010000000
VERSION     DACKAC010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.103"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            54..506
                     /locus_tag="I0B46_002572"
     CDS             54..506
                     /locus_tag="I0B46_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084814.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            520..1209
                     /locus_tag="I0B46_002573"
     CDS             520..1209
                     /locus_tag="I0B46_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084815.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1334..3685
                     /locus_tag="I0B46_002574"
     CDS             1334..3685
                     /locus_tag="I0B46_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAR2084816.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3765..4160
                     /locus_tag="I0B46_002575"
     CDS             3765..4160
                     /locus_tag="I0B46_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084817.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4485..5102
                     /locus_tag="I0B46_002576"
     CDS             4485..5102
                     /locus_tag="I0B46_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAR2084818.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5143..5442
                     /locus_tag="I0B46_002577"
     CDS             5143..5442
                     /locus_tag="I0B46_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084819.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5476..5643
                     /locus_tag="I0B46_002578"
     CDS             5476..5643
                     /locus_tag="I0B46_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAR2084820.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5688..6251)
                     /locus_tag="I0B46_002579"
     CDS             complement(5688..6251)
                     /locus_tag="I0B46_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAR2084821.1"
                     /translation="MIFKLRNRTEGLNIDKLFQEKASGKDTERPEFQKLLNYIREGDC
                     VIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLDTAMFDMFLSL
                     LSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLVFKTVVDMLKQ
                     GIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2150 a          864 c         1252 g         1989 t
ORIGIN      
        1 tatcttatcg tagatatata ttataaattt aataagaaag cgaggtgaga atcgtgaaaa
       61 aaacgatact aagtattttt tcagtattta caatattaat tgggggctat tttcttttga
      121 atttagattc tgttaattca aacgctcaaa taaaagatca aagagtagct gaacataata
      181 ataaaaaaat gagtttgacc aaaaatatct attctatgtc agatgaagaa ttgtataaag
      241 aatttggtga tttatccggg gaacagttag aagaaagtat tccaggaatt agtgactcag
      301 ataaaattgt tgtcttgtct gctggtgggc aattaatggg agatggcaaa tccgaattag
      361 ttagttataa acaaaatcac agacagttta ttgatgttaa tactgattcc aacgctattt
      421 cgattaagga tctaaaagag cttttaaata gtaaaagaaa tgaattaaag cagataaaag
      481 aaactaattc agtggaatta gagtaaagga agattcatta tgaaaaagat ttttggtgtt
      541 tctgttgggc tacttatgat gttaacattt ggaaatgcag tatatgctgc tgagaaagaa
      601 gttatttcag atccagagca gtacgcctca cgatactttg ctgattttga agaagaagat
      661 aaagtacttg cattgccaga aggtggtttt ttaaaagggg aagctaaaat tgtatctatg
      721 gataatccag atataattca aggttcatat aattcagaaa ctgatcctgc ttcagtttca
      781 gtgacagaag caaaaaaaat tgttgctgaa aaattcaaaa atgatattga aactactcca
      841 attatgcctc gaggagcatt tccacccgat gttggtacta gcggacacct tcttgtacta
      901 gcaggaggag gaagctataa atcacagcca tttagtggtg cgggatggcg ttttggaggt
      961 atgggtgtaa aaccagctga tggtacaggc aactttttac gttggaaatc ctacattgac
     1021 gatggaagag taggaaatgt aaatcaagcc aacgcaacta aatctggttc tattcaagga
     1081 atggcattgt atgctcgtga aggattcaaa tactttgcac caaacgggcc tggttggaca
     1141 atgaatatag gtatttatta cacttataat cctgttgcag gtacgtatta tgttgttcaa
     1201 aatatttaat aaaacagcac aaagagattt ctttgtgctg ttttattatt tttagttgtt
     1261 gacaaacact aaaatataaa atataatcaa cacataaacg aacgtatgtt cggcaagaaa
     1321 gcaggggatt tttatggatg agatggtttt aagtactcag aaatggctaa acaagaaata
     1381 cagtaatgtt acagggtttg ataaggttcc agaaaatggg cgaacagggt ggcctacaat
     1441 ctatggactt attgaaggac tacaagtaga acttggtatt actaatctag tagctaattt
     1501 tggacctacc actgaaaaaa tgtacgataa tcaggttact cctaaatggg gcaaaaatct
     1561 acctaaaaat attgtatttc ttattcaagg tgccttttgg tgtaagggga ttaatccagg
     1621 tggcttcgat ggggtatata ctccatattt agatactgct gtaaaagagt tgcagactga
     1681 tgcagggttt aatggaagcg ctgtaactgg agttttggat gcgaaatggg ccaaagcgtt
     1741 atttgatatg agtgcttttg ttcttgttcc cggcggagat agtaaaatta gaaaaatgca
     1801 gcaatggtta aatgtaaatt atttagaata cacgggaata atgccttgtg atggtattta
     1861 tcaaagaaat tcaaatcaag cattaatttt tgcattacaa gctgaactag gatattctcc
     1921 cagtgaagca acagggtctt atggtaatgg gacaactagt aaaacttatc ctgtgtctga
     1981 aggtaactcg ggaaactatg taagaataat tcaatatgga ttatatgtta atggttattt
     2041 tgaagacggt acattcgatg gtattttcac taaatatatg ggtttagaag tactggcatt
     2101 ccgaaaattt atggtattgc ctgaatatac tgaaatagca gatgttgttg taataaaagg
     2161 actattgtct agcgctggag atattagaag atctgcagat ggcgcagata cttctacgca
     2221 attaactaga tctcaaatcc aaacattggt tgataatgat ataaaaatag ttggtagata
     2281 tcttactggt actgttggaa ttggaaaaga tgaacgaaat aagtatctaa ctacagagga
     2341 attgaataat attttttcta aaaatttatc tgtgtttcct atctatcaag atggaggtgc
     2401 agcattagct tattttactt atgatcgtgg cttatcagat ggtaagaagg ctatagatgc
     2461 agcaaaaaat ctaaatatac cattaacaac agttatttat tttgctgttg atctagacat
     2521 gttaggggaa gaaattcttg catatgctgg agaatatttt aagggggtat ctgctgtaat
     2581 gtcatatagc gggtatcaaa cgggtgttta cggaacaaga aatgtttgtt ctcaaataat
     2641 caataacggg tatgcttcgt ttgcatttgt ttcagatatg tcgactggtt atagtgggaa
     2701 tttaggtttt cctatgccta gacaatggtc ttttgatcaa tttgttgaat ttaccatagg
     2761 aagtggcaac ggagctgttg gtatcgattt aatcgctaca tcgggtagag atagcggatt
     2821 taatgagtta tcaaatgata ctaataatga taattacatt gccaaataca atcagaaggt
     2881 tataaatcag atggtacgtg cgtatcaata tctatcacaa gctggtcttg ataatccttg
     2941 gaaccctcat ttgacatttt atagatatgt aaattattct ggtttgtcat gggacatcat
     3001 ttcttctcct gtaacagagc atgatagaaa aatttatgat gaatatagga ataaattaac
     3061 taatagcgag ggcctgtata attattttat agatcctaat actggttcaa taataggttt
     3121 gcctcactta attgtgacgt tacaatcaca gatgtttcta gccgacacaa tcaatgatag
     3181 tgtttctgac tttgcaggtt ggttaggaga tttaatgact tgctggggtg aagttaagaa
     3241 attaggaata caaatggagc aaggtgtatt tgcattagta ggtacggctg caggtggaac
     3301 atttagtcta gaagatttat accaagatgt tgacgcggta aatgtttata ccttagcaaa
     3361 aagaaattct gattcaacag ctttatcggc tttaaaaacg tactattgtg ataagggtgc
     3421 tattgataga tttaatcaat ttgttaagaa cagatttggt tcttcagata aaatcgcttc
     3481 agtaacaaat agtttattag aaaatgacga aacagttgat atcttaggtg tagggataat
     3541 ttcatttttc attgataaaa tagttaataa ttcacttggg acagatttta cttatcttga
     3601 ttttattgga gatgagggga aagttgcaaa tggattttct gaaaaaatta ttcaatttca
     3661 agaaaataga agtaaactta attaagaatc gtttactatt taaacagtgt ccttttcctt
     3721 tttaaatata tacgataaaa taagtatatg aattgtaaaa ggacatgatg attatggaag
     3781 aaatagaatg gaataatctg actcaaaata ataaaaataa ttctggatgt ggaattatct
     3841 ttatgtttgt tatagttttg ggaatagtag tacttttaat aactgtcaag atagtagatt
     3901 actttgcgaa tactcctgaa gaacaaatac agagaaaact tcctgagttt gaaagagtac
     3961 caccttatga tccaagagat aattcatttc aagtaaaaaa attaaaagag tctccaaatg
     4021 aaatgtggtt tgaagttaca gttgaatcaa gcgacttcaa agaaaaaaag aaagaataca
     4081 attatattgt aaaggcaaaa gtatcaaaaa gatttatttt taaccctaaa ttagaaatat
     4141 atagtgttgt aatagaataa tagtgttaaa aaaattgtaa aagttaaaga agtcaaattc
     4201 tgatgtgaaa aataaaatat accatggaag acataaaaga aaaactggat aactagttta
     4261 tttaatggca gaccgttaag gcctgccatt tttatgtatt ttagcggaat aaatataatc
     4321 atgcgggagt gtaaacttac tgtttcaaaa agtatacttt ttgacatgcc ttgattttct
     4381 ttgtttatgt atggcataat aaccacataa tacaatttga aacgtttctg atttaatctg
     4441 attgttttga aacaatttta gtgaaaaaag ggtagataaa aacgatgaga aaattttttt
     4501 atatccgggt cagtagttat gatcaatcga tcgaacgaca actgatttcc gcgaaggaat
     4561 taggaatccc tgaagaatat atatttgtag aaaaagccag cggcaaagat tttaaacgcc
     4621 ctgaatatca attaatgaaa cggatgtttc gaaaaggaga tttactctat ctacaatcat
     4681 tagatagact aggcagaaat aaacaaatga ttttagaaga atggcgagaa ttaactaagg
     4741 aaaagcagat tgatatttct gtgttagaca tgcctctgtt agatactgct agttacaagc
     4801 aactagatgg gttagagacg cttatctctg acttaattct gcaattactc agctatatgg
     4861 ccgaagacga gcggaaacga attaatagcc ggcaaagaga aggcgtagaa gctgcgaaac
     4921 aaaagggtgt aaaattcggc agaaagaaaa ttgctttacc tcctgaattt cctgcgatct
     4981 attctgactg gaaagcagga aagataactg cagtctatgc tatggatcaa ttgggcatga
     5041 aacgaaatac cttttatcga cgggtgaaag aatatgaaca taccttgcac cacttttctt
     5101 gatagatata acagctatta gacgggaagg gagagtttga aaatggactt aacgaatcaa
     5161 agggtcgttt ttactggaac gctgcagcaa atgacacgaa cccaagccat cggtttggtt
     5221 cactctttaa acggccattg tcaatcaagt gttacttcaa aaacaaattt cctggtttgt
     5281 ggtcagtatt ctaagtcgtt gtttgatgat tctctctata caaaaaagga acaaaccgcg
     5341 cgggacttga ttgcgttagg tcatgagatt accattttat cagaagtcga attttatgcg
     5401 ttaattagtc aacagttgaa agaatggcaa cggtatttat aatcgttagt aaaatttaca
     5461 aggaggaaaa tttttatgaa gcgaaaatct tatgatcgtt ggggcattga aataactagt
     5521 tttaatcgaa atattgcttt tcacgtggag tgtgactgtg gggaattagc aaaaagtgtt
     5581 tatcaaaaaa attactttta ctgtcctaaa tgccatcgcc gatacggggt acatggaaca
     5641 tagaaacgaa atagtgcggt agaaaagcga atgaatgtag tatatttcta caaatcattc
     5701 gctttcttta ttttataaat agttggtcga gagatcccgt tttcttttgc aatctctgca
     5761 actggaatgc cttgctttaa catgtccacc acggttttaa aaactaagcg tttttgcgga
     5821 tctgttgagt taatagaata ctcgattggt cgtcccttat atcggccggc tttttgagcc
     5881 aataatactc cttgccgctg acgttcacga attttctcac gctcgttttg tgcaatataa
     5941 cttagcagtg ataaaaacat atcgaacata gcggtatcca ggagttcatt tccagtatta
     6001 aaatctaaaa actgtgcatc taatattttt aacgctactt ttttctgttt cataaaagca
     6061 actgtatttt ttatatcttc ataatctctt cctaaacgat ctagtgatgt aacgatgacg
     6121 caatcacctt cacggatata atttagtaat ttttgaaact caggacgctc agtatcttta
     6181 ccactggctt tttcttggaa taatttatca atgttaagtc cttcggttct gttgcgaagt
     6241 ttgaaaatca aacgc
//
LOCUS       DACKAC010000102         6095 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.104, whole genome shotgun sequence.
ACCESSION   DACKAC010000102 DACKAC010000000
VERSION     DACKAC010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6095
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.104"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(366..1157)
                     /gene="msrA"
                     /locus_tag="I0B46_002580"
     CDS             complement(366..1157)
                     /gene="msrA"
                     /locus_tag="I0B46_002580"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAR2084822.1"
                     /translation="MLDVEQMEIDLYNIILNRNTREWERTQFEEAKEDLAKMSIKQVI
                     EKLEVNLRPLALRDNLTPDAMDFYLRITGESASAKAYDYAKHKITSPLYQERAIFAGG
                     CFWCMVEPFETKPGVISVLSGYTGGETKKPNYDQVSGGYTGHVEAVEIIFDTRQITYE
                     ELLEIYWQISDPTDAFGQFQDRGKQYRSILFVVNEKQRQLAEKSREQLIQSNRFSRPI
                     VTEIRQAKTFWPAENHHQQFYQKQPKRYKKIKHSHQLHLFFQRHR"
     gene            complement(1348..1983)
                     /locus_tag="I0B46_002581"
     CDS             complement(1348..1983)
                     /locus_tag="I0B46_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAR2084823.1"
                     /translation="MIFLQKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFIGTSKTLAILAVATVGLYGIGDCIFT
                     GLFSINTNESSWNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(2249..3301)
                     /locus_tag="I0B46_002582"
     CDS             complement(2249..3301)
                     /locus_tag="I0B46_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAR2084824.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(3314..4252)
                     /locus_tag="I0B46_002583"
     CDS             complement(3314..4252)
                     /locus_tag="I0B46_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAR2084825.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            complement(4703..5353)
                     /locus_tag="I0B46_002584"
     CDS             complement(4703..5353)
                     /locus_tag="I0B46_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084826.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            complement(5453..5962)
                     /locus_tag="I0B46_002585"
     CDS             complement(5453..5962)
                     /locus_tag="I0B46_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAR2084827.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILEVFAEAELYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
BASE COUNT         1937 a         1274 c          895 g         1989 t
ORIGIN      
        1 atagttattg tttgcgcttt tatacggaat ttagttcatc attaatgtgg aattcgatta
       61 ggtgtctatt aattatcaag atgattctct acaaaattcc atagaaaaat caagattcta
      121 caattcgaat tctctttcac atggaaataa aggctagtaa aaaaacagga gttgtgacaa
      181 aagacttgtc atacctcctg tttccttgcg acttgaagct gaatgctttg cgccacaacc
      241 ttttcctcgg tgttctcaaa gcaactcctc gaaataattc ataactgtcc aaaaatatga
      301 aaagctattt ttgtcagttg ctcattattt tgtcggagct gaacgctttg cgccacaacc
      361 ttcctctatc tatgtcgctg aaaaaataaa tgaagctgat gcgaatgctt gatcttttta
      421 tatcttttag gttgcttctg ataaaattgt tgatgatgat tttcagctgg ccaaaaagtc
      481 tttgcttggc gaatctctgt aacgatagga cggctaaatc ggttagattg aatcagttgt
      541 tctcttgatt tttcagccaa ttgcctttgc ttttcattaa caacaaatag aattgaacga
      601 tattgtttcc cccgatcttg aaattgacca aaggcatctg tagggtctga aatttgccaa
      661 taaatctcca acagctcttc ataggttatt tgacgtgtat caaatatgat ctcaacagct
      721 tcaacatgcc cagtatatcc accactgact tgatcgtaat ttggtttttt ggtctctcca
      781 cctgtataac cagataacac tgagattaca cctggttttg tttcaaaagg ctcaaccata
      841 caccagaaac agccaccggc aaaaatcgcc cgttcttggt ataagggtga agttatctta
      901 tgtttggcat aatcataagc ctttgcactc gctgactccc ctgtgattct taaataaaaa
      961 tccatcgcat ctggtgtcaa attatctcgt agtgctagcg gacgcaagtt gacctctagt
     1021 ttttctatca cctgctttat cgacattttt gctaggtctt cttttgcctc ttcaaattgt
     1081 gttctttccc attctcttgt atttcgattt aaaataatat tataaaggtc tatttccatc
     1141 tgctccacat ctaacatgaa gtcaccctct ttcaagtttt cttcttattc attcaaaaaa
     1201 tgactacctg aaatttagca caacgcataa caagttaaga ctaacttgct tttcgctctc
     1261 ctcatctcta ttcttagtcg aacacatcca cacgaatggt tacccataga gaaattcttt
     1321 tacttaatca gcttcacact gctttttcta ctttcgtagt tgaaaaaaac ttaaacaaat
     1381 catcggcaaa tagttaaaga aaaaacttaa gcgttgaaag aagcccaatt gcttaaagaa
     1441 ggcaaactga ctaatcgacg gaatccgtgc taatccgtaa agacctgcaa ttagtaaact
     1501 aataactgtt aaaacaagat aaaaatggct aagcgtacct tgaccacttt gacgataaag
     1561 cagaacaagt aacaatggga aaagtagaaa gcctgcatag cctagcccag aacctatatt
     1621 atggatccaa gtcgatagat tccaagagct ttcattcgta ttgatactga ataacccagt
     1681 aaaaatacaa tcacctatcc catataaacc aactgttgca acagcaagta ttgcaagtgt
     1741 ttttgatgtt ccaataaata ggtgatacaa cgcaggcaac gaaagaacaa aaaaaagtcc
     1801 agatacaact gaccagacca agaaagcacg tctgactgga ctatctacgt caccaaacac
     1861 actaattacg gtagtcattt gattcagttt gggataaaaa aggccaagga tatacggtgt
     1921 cgataaatca ctaatcacac ctaataataa aaaataaaaa ccatattttt gcaaaaaaat
     1981 cattttcttc tcctactttt tagtgattat tcgatacatc caacaatcaa attttttctc
     2041 attttaacac atatttttat tctagttgcc ttttgaacaa tcaataacat aaaggtgagt
     2101 ttcttctaca ttttaaaaaa tcgaatggca ctgaagagtt cagttgtcag catcaatctc
     2161 ctactcaaaa aggcaaaaaa acgtaagtca gatgttgttg acatctcact tacgcatcca
     2221 ttgattgaaa atttcttagt aaaatctact agcctaaaaa tttgaagact tcttctcgat
     2281 catacagttt aaatactttc tcgcataaaa attgagtaat tagtgccgca aaaacaggga
     2341 tgactaacca acaaagaaca acaagaaagc catttagccc tgcatctaaa gaagccaacg
     2401 gaccaacaag tccgaccacg ccgaaaccag cagaagcagg cgtacctgaa atagaaaata
     2461 gagcgacagg aatagctgta atcactgcac taactaaaca aggaataaga atcacaggtt
     2521 ttctaaatag attaggcatc atcatcttca tagcgcctaa agcaatcgca attgtcacac
     2581 ctgacttatt aaccttccaa gagttcacta ctaaaacaaa agtggttgca gctacgccca
     2641 tcgcagccgc accagcagaa acaccatcta gttggattgc cattccgatt gctactgttg
     2701 aaataggaga gataatcaaa aaggcaaagg aacaaccaat caagatagac attaacagcg
     2761 gttgtaaggt agtgaagtta ttaatcaatg atccaattgc agaagtaatt gctgttacaa
     2821 aagggaataa ataaaaacca agtaaacctg cacctacacc tacaacaatc ggtgttaaca
     2881 caatcgacac tgacccaaaa ctatttccaa tcagtaaaat cagcccgaca gcaatcgatg
     2941 ctgtcagcat cgtattaata atatcgcctg taccagcacc aacgtaaaca cccgtttcac
     3001 ccaaatcagg tgtgaatttg actacaccag aaccaacaaa agaagctccg gctacaacca
     3061 tcattctcat cggatcaaat ccaaattgta aggcaatcaa accaccaatg atcaaaggtg
     3121 ttgctagttg aaaaataact gctaactgtg cgatcgttac cgcaaaggga tacgcactaa
     3181 aatattttaa aatggcacct aatactgcat taggaatcaa tccaataatg attcctgttg
     3241 ctgtaccagc gaggacttta ttcagaaaaa ttttcggtga tattttttgt gaagtttcca
     3301 ttcaatctct cctttatttt acgttcaaac tatcttcttt tgcatggaca agctcagtca
     3361 agaaatcaca ataaggcgtc gcaacaccat atttcttccc ttttctagaa attgcgccat
     3421 tgatataatc gatttctgtt aatcgatggt tcttaatcaa gtcttgatac attgaaggat
     3481 aatgtaaccc aattgtttcc gggtcaaaac aagattcaca atgtgcaatg acttctggga
     3541 catctagttc aatcttctct actgctgcta ctttggcaaa ctcattgaca atcgtcgcca
     3601 ccattttatg agcagtcgat gtttttccta gctcagccat attaacgtct aagattgtgc
     3661 atagtccgtt cattgttccg ttcacacatg ctttgcgata aatagaatag tgaatattgt
     3721 cagaaaaatg agcatttaat cctgattcag acaatttgtc tgctaatttt tttgcagctg
     3781 cttcttttcc atcacctaaa ttttgtaatt ctactgaacc acttccaaat aatttgacct
     3841 gaccaggacc ttcaagacct gccgtccaca tggtatttcc aatatagata ttttccattg
     3901 gtacaaattt ctcaataata tcttcatgcc cgataccatt taatagacat aagacctctg
     3961 tatctttttt gattaatgat tggatatcct gcagcatttt ttccagctgc atcgctttgg
     4021 taaatagaat aatcagatca acttgatctt ctttttctac ttcggattga agaacgattg
     4081 gtagttttgc ttctacctct ttaccattaa aatttgcttg taatccatgc tctttgattt
     4141 gttggacatg ttctgcccag ccatcgatta ataatacttc gttcccactt tggtgaagca
     4201 ttaacccaaa gcgacttccc attgcgccag cgcctgcaat tgctattttc attgattctt
     4261 cctccttttt ctttctatta tattcatttg agaatcattt cacaaccttg ttttgttatt
     4321 cttctcttat ttatcatgtt tatatccacc attcaagtta ttttatcagc aaatcaataa
     4381 aaactggaat agaatcccaa tttcatcgta agagaaacta gactagcaat aagaaaatag
     4441 aaacattcat agaaaaaacc tcactcctct gattataaaa gagggggcat taacctcgtg
     4501 tatactaaaa ccttccattc attcgatgga tgattttctt tttgtgaaaa aatattcatt
     4561 gtagtacgat gttgcgcaac acagacggat ggtattgctt attatttatt aaaatcgaac
     4621 aatacactta tttaaaaact tctttcccag attttcttcc atttttacag ttataactag
     4681 tattatcatg aaaaatagcc ttttattgtc tatgggaata ataatttaaa attggtactt
     4741 ttttattaat tagttcatcg cccaataatc tctccagttt cttagcatct cctgactcaa
     4801 ccggaattat ccaagcccag tccaaataat tttttaaaat ctcatcatat tttgcaaata
     4861 tttcattgcc atattccgtc cttaaaatta tattttcagt gccagacaag tgatttgtat
     4921 tgagttgata aaaaagatct ccatctaaaa aaatatcatt agaagaagat ttccccacag
     4981 taatgaccat tccatcagct ctatgtgtta catacaaata attgtatgac ctcatttcac
     5041 ctaatgaatc aattatttct tgccctttag ctctaatata ttgatctcta ttacctatct
     5101 cactcttatt taacaaaagt tttttcatgt caaatttaat cggctgtttt atttttgtaa
     5161 attcgcttct actactaatc caatccgaga ttaatttttc aacatgtccc agagtaaact
     5221 ctgatttatt cgctaaatac tttttttgtt gtattaaaga catctttccc cgaagctcaa
     5281 aattgagcct acatcgattc tctatctctt caaaaagcat cttatcttca tcattcttat
     5341 attcatcatt cataaaacca atccagctcc tcgattaaga taatatttta atctcataca
     5401 aaaactagct ctcatcaaaa caaccattgt tttgatttct catgaaattt ctttatactt
     5461 ttctccgatt ataaattcct aaccccttga gaaaattacg catatattta tctccgcaag
     5521 gcttaaaatt tcgttggcct tcttttctca aaaatgcccc gatttcgcct tttgatggat
     5581 agagttctgc ctcagcaaaa acttctaaaa tatcatcagt ggtcatagac aaggcgattt
     5641 ttaatttttt tagcaacaca ttgttgatat atttgatttc cttgcttttt tgcggcgtgt
     5701 ctactatcgg cttatctttt tgcggaccac gcgccaagag aatcaggcca tttaggaatg
     5761 cgtcaaaaac ttggttgttg atggtttttt catacacatt attttctgca ttatctttgt
     5821 tcttatcctg tactttggtc agtaccgcct gagcttcttc tttagtcagt ggtacaccgc
     5881 ctagctcaaa agctttaatc aaatccgtat ctttcaaatc aatcgcataa cgcaatcgca
     5941 ataacctatc attgttattc atttgttcct ccgaagtatt tagtctttac tttcttacgt
     6001 actttttgtc ttgctaagat ttttaggatc aatgataaaa accgcatcac ttatgatacg
     6061 gagctgggta tcatgagtga atgatattta taaat
//
LOCUS       DACKAC010000103         6068 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.105, whole genome shotgun sequence.
ACCESSION   DACKAC010000103 DACKAC010000000
VERSION     DACKAC010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.105"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            832..1566
                     /locus_tag="I0B46_002586"
     CDS             832..1566
                     /locus_tag="I0B46_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAR2084828.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1582..2751
                     /locus_tag="I0B46_002587"
     CDS             1582..2751
                     /locus_tag="I0B46_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAR2084829.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2767..3762
                     /gene="lacD"
                     /locus_tag="I0B46_002588"
     CDS             2767..3762
                     /gene="lacD"
                     /locus_tag="I0B46_002588"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAR2084830.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3841..4767
                     /locus_tag="I0B46_002589"
     CDS             3841..4767
                     /locus_tag="I0B46_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAR2084831.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4786..6036
                     /locus_tag="I0B46_002590"
     CDS             4786..6036
                     /locus_tag="I0B46_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAR2084832.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         2027 a          997 c         1247 g         1797 t
ORIGIN      
        1 attggcgcag cgaatccttg ataatgagct cggatgtgct aacgctagcg aggcagtcta
       61 actttaactg ttagctcctg ccgaagatcg tcacatccga ggaggctcat tgtcaaggac
      121 aatcggaata gcatacggaa agaagttctg caccttataa aatttgtcaa aaaaactgtt
      181 gccattccaa agtttgaaaa atgctatttc gaggtagaag gatattatat aagcacggtt
      241 gactaaacga agtgataata aaaaaagtta ttcaagaata agaaaaatta tgatacgaat
      301 tcagataaac tggagaatac agagaccttt aaaagggata agaaacaaca taaattcctc
      361 ttaagagaga acgaaaggac aaagccagca acttgataca cgagctagta ttagaggaat
      421 ttattctcat agactccctt ttgaatacgt ttcaacttta attttcatgt tcataaatga
      481 tttgaaacca aagcaaatgc gttgcacttt tcctccgtct tccgaaaatc gttcaattca
      541 ctggtaagta gtcatttgat agtttgaggc aaattgttga ttgatgaagg gtaggagcta
      601 aaaaaagata ccagttgaag agagaaaaag aaaaaaatag agggtgttct tgaatgaaaa
      661 aagttggaaa attatttttc atttcctttt attcctatta aagtcagtga tgtcaatcat
      721 attaacaaga ctaaaaatga aaataaaaaa aagattgttc ttagtattga ctcataaaaa
      781 ataccactac tatacttaca ttgtggttat agccagttag gagagacatc gatgcaaaat
      841 acctttcaat caaaatcact ttattatcag ctggtagaaa tattgaaaca acgaatcgaa
      901 aacagtatgg tcgctcatga caagctgcca tcagaaagag agttgaccag tttttatggc
      961 gttagtcgca cgacggtgcg actagcattg caagaactgg aaaaaatggg ctacatttat
     1021 cgacaacatg gaaaaggaac ctttgtttcg gaattaagga caaatgcgac tgatctagct
     1081 ggcgcatata gttttaccga acaaatgcgg gcaatgggca aacatcctga aaccaagata
     1141 ttggccttca agaagattga agcaactaaa tatttagcga gtcatctgaa tgttcaacta
     1201 ggtgagcctt tatttcaatt agaccgattg cgcttagctg atgatttgcc aatgatgatc
     1261 gagaaatcct atttgccagt gaagctattt atgtcccttt ctcaagaaat tctatttctg
     1321 aaacctttat ataatttgtt ttcagagaat ttcaatcaaa tcgtacggtt ggctgacgag
     1381 gaattttatg ccagcattgt ccagaaagaa gatagtgagt tgctaaatat tccagaagga
     1441 tcaccagtgc tccatttagt tcgctcaacc tataatctcg agaacgaagt gattgaattt
     1501 acattaagtg tagcgcgggc tgataaattt cattataaga tcaggcatct gcgttccact
     1561 aactgaattg gaggaaagaa aatgtttatg ttctcaaaag aagaactacg aaagctagga
     1621 gcagaaaata ctacccgaga aattcgacag caaccggaac tgtggcatga gacgtttgac
     1681 cgttatcaag aaacagttgc agatttagat cgtttgttta cggatgcgaa acaacatgct
     1741 ggagagcgac caatccgagt catttttaca ggggccggaa cctcgcaata tgtgggagat
     1801 acactaattc cgtttcttac tagtcaagga aatacgaacc agtttatttt tcaaagtatt
     1861 ggttcgaccg atatcgttgc tacgccaaaa gaatacctga ttcctgatga gacaacgatc
     1921 ttggtctcat ttgcccgtag tggaaatagt ccagaaagtc tagccgcagt taagatggcc
     1981 aatcagctgg tcaacaagat ctatcatatc gtgatcacct gtgcaccaga cggattattg
     2041 gctacacaaa tgcctacaaa aaaagaaaat ctagttttac taatgccaaa caaagccaat
     2101 gacgctggtt ttgctatgac aagcagtttc agctgtatga cgctgactgc gctacttatt
     2161 tttgatcaag caagcaatca gcaaaaagaa agatatgttc atcaattaag ccgactagga
     2221 gaagaagtca tccaacgaga atcagagatc caagggtatc tggcatcaga ctttcatcgc
     2281 atcatttact taggttcggg aagcttaggc ggattgacta gagaagcgca attaaaagtt
     2341 ctcgagttaa ctgctggaaa aatcgcaacc gtatttgata catctttagg attcagacat
     2401 ggtcccaaat cttttgtcaa tgagcaaaca ttggttttcc tgtttgtcag caatgactca
     2461 tatacgcaga aatacgatgt agacatgctg gaagaaatca aaaatgacgg tattgcccaa
     2521 cgcgttgttg gaattggtca aaagggttca ttgtttagtg gtgattcctt tgaattagtt
     2581 gcagattcgt tactgccgga tggctatctt gctttagcag atatcatggt agcccaaacg
     2641 atcgcattac tttgttcggt gaaggttggt aatacaccag atacaccatc gccaagtgga
     2701 acagtcaatc gtgtggtcaa aggcgtaacg atttatccat atgaaaaata ggaaagagtg
     2761 tgaagcatgt tagaatggtc ggaaaataaa agacaagcaa tggaacgtct atcgacgcca
     2821 gaaggcatta ttaccgcttt ggcgatcgat cagcgaggcg cgctgaagaa aatgatgtcg
     2881 gctttaggga tggaagcgac aaaggaaaaa attgagcagt ttaaaacact aatttcccaa
     2941 gaattgactc cttatgcctc agcaatttta ttggatcctg aatacggact gcctgctgca
     3001 cagaagcgag cagcagcgag cggtctgctg ttggcatatg aaaaaactgg ttatgatcaa
     3061 accgtacccg gccgcttgcc ggatctgctg tcggactgtt cagttctgcg attgaaagag
     3121 ttaggtgcgg atgcggtgaa atttttactg tattacgatt atgaagaagc agaggaaatc
     3181 aatcggcaaa agcaagtttt tgttgaacga attggaagcg aatgcatggc tgaaggactg
     3241 ccattttatt tagagttatt gtcatatgat gctaaaaatg tagatacgaa atcgctttcc
     3301 tatgccaaag caaaaccgcg caaagtgatc gaaatgatga aagaattctc gaaaacccag
     3361 tataaagtag atgtcttaaa ggttgaagta ccagtaaaca tgtcatttgt tgaaggaaat
     3421 gcaacagggg agatcgccta tacacgtcaa gaagctgccg cttattttaa agaacaaagc
     3481 gatgcaacag atttgccgta tatcttctta agtgctgggg tatcagcggc attattcatg
     3541 gagacattgc gttttgccaa acatgctggg gcacgattca acggtgtact ctgtggcaga
     3601 gcaacttgga aagatgcagt cgctcctttc gttgaaaaag gagaagcggc aactttagaa
     3661 tggctgacag aacaagggac acaaaacatt cgtcaattga atgaagtgat aagagaaact
     3721 gccatcccat ggtatgagaa agtaagtgaa agcacttgtt aatgtttcta atatattagt
     3781 agtgataaga gatatgtgtg attataattt atcattattg aaactgggag gatattttat
     3841 atgaataaaa gattgttaga ttattcagct tcagattttt tgaaagctaa gccgatggaa
     3901 ttaaagcagg caattttggc ttctgaaggt agaacaatta tggcagaaaa tgtgccaagt
     3961 agctgtcctt acatgggaaa atcagtgaca aatgcagaat tggagagagc tgctggagct
     4021 gatttaattt tgtttaatgc actagatctt tttaatcctc aaatttcagg tgtaccttta
     4081 actctaaaag agaatccaat aatctggatt aaaaaagctt taggaagagc tattggtgta
     4141 aatttagaac ctgtagacaa agaagttttg atgagtgaaa atcgaactga aatttcaagt
     4201 ggtagaatcg tttctcctga gactttaaaa aaggcaaatg aattaggctt taattttatt
     4261 tgtttaactg gaaatccagg aacaggagta tctaatagaa ctattgagaa agcaataaca
     4321 ttatccaaat caatttttaa tggattaatt atcgctggaa aaatgcatgg tgctggtgtt
     4381 gatgaaccaa ttatgaactt agatatagca aaaagattta ttaaaacagg tgtagatatt
     4441 ttattagttc ctgctccata cactgttccg tatttcatgg aaagtgattt gcgaggaatc
     4501 gttgattata taaaagattt taatagaaat aaatctattg aggaaaaagt tctagtacta
     4561 acggctaatg gaacaagcca agactcttct gaccaagaaa ctattaaaaa aattgcatta
     4621 gcatcaaaag catgtggagc tgatattcaa cacatagggg attctttaaa tggtatttgt
     4681 cttccagaaa atatttttgc tttgggtcaa gcaattcggg gatatcgtca tcaaattatt
     4741 atgttaggaa aatctaatta tcgttaatta aggaggaaat gaaagatgat cgacaaagtt
     4801 caaaaaatag ctgcaaaaat tagtaatcaa agacatctac tagctttaag agacagcatg
     4861 gctgcagtta tgccattaat cattattggt tcctttttta tgttattagc aaatttcccg
     4921 atagaaggat ttacaaaatg gttatctgaa attggagtac aatccttttt gaacaaagca
     4981 tcagattcaa catttggtat tattggatta gctgttacct actctttggg acacaatcta
     5041 gcaaagactt atggtgtaga ctcattatca gcaggattat tatctttgag tggatatgtt
     5101 ttacttacac ctttattgga tgcagatggt tctataggat ttccaacaaa atactttggt
     5161 acctcgggtc tttttgttgg tattattgta gcacttgtat ctacagaaat tttccggtgg
     5221 tttgtactta aaaatattac aattaaaatg ccggcaagcg ttccaccaaa tgtaagccga
     5281 gctttcgttg ctattattcc tggctttttt gtcgttctac tatggtttat tgttgttgta
     5341 atttgttata aacttggaat tgaaaatgtt catgctctta ttgcagatac actcgcaaaa
     5401 ccactatctt tactgacgaa aactttacca ggaattatat tggttatact tattcaatgt
     5461 ttcttttgga tgtttggtat tcatggtgca caggtaacgg ggccaataat tgaaccgtta
     5521 ttattgcaaa actctgatgt aaatagaatt gcttatcaag ctggaaaaga acttcctaac
     5581 attataactt atgaattctt atataacttt gttttttctg gtggtgcagg ttgtttattt
     5641 gcacttgcca ttttaacatt tttcttttca aagagtcagg aaaataaaac tttaggtaag
     5701 ttatcaatcg ctccagtaag ttttcaagta gcagagcccc ttctatttgg gtttccaact
     5761 atattaaata taaaaatgtt aattccattt gtaactgcac cagtagtaac aacattaatt
     5821 acttattttt caatgagtat gggattagtt gcaaaacctg taggagcaac aatcccatgg
     5881 acaactccac cgataattgc cggattttta gcttctggtg gacgaataag tggtgcagtg
     5941 attcaagtaa taacaattgc aataaatgtt ttgatatact atccattctt taaattagat
     6001 gataatgcca agctaaaaag cgaaaaaaat gattagaact acctatatgt ttctaactgt
     6061 agttaagt
//
LOCUS       DACKAC010000104         5967 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.106, whole genome shotgun sequence.
ACCESSION   DACKAC010000104 DACKAC010000000
VERSION     DACKAC010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.106"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            71..481
                     /locus_tag="I0B46_002591"
     CDS             71..481
                     /locus_tag="I0B46_002591"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084833.1"
                     /translation="MVTLFYRRDFLFSIYTKYFTLSSNIKTSTKLICKLFKLDKISNI
                     DDVKSKILTRKVSEPVKLTAQDKKNGIVLYGDDAKYNYNWDTSSEAEVLSIPTTDTSG
                     EKVTFKINFITAISKLEYGKQKKRDNNDQYLPKK"
     gene            562..1182
                     /locus_tag="I0B46_002592"
     CDS             562..1182
                     /locus_tag="I0B46_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084834.1"
                     /translation="MNINRVIKLIGAKNINKVRSEFIFPEDFFNWFFYQFSECKRLLG
                     ENYYLKGIGGFMGNVADEQNIIKGISEQTSELTVTKAFISNGESLKNVTARIRKENEV
                     EIVFGIDHECNSVIYVTQSANLILFENGCKDDFMVVYLYDILIPKLRSLYMSSESIFV
                     SEQKGSFSKKIGIEVIESIMCHNHITLEELEILIGQKNNNTKIQIS"
     gene            complement(1512..2738)
                     /locus_tag="I0B46_002593"
     CDS             complement(1512..2738)
                     /locus_tag="I0B46_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAR2084835.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGITSEKYQKVFPTRQLAIKA
                     ENAMKRKIETVLREENANSLELKGKIKFKEFYESKWLPRYELGQTTRSKRAPSYVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQCITAPKKIALEERRIREGNQALSSEELIAWIEAVNTDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFEKQTVRLKHALDKYQKKKFTKGRKDTV
                     IQVPEIVMALLSKWKSVQAEQLLRLKINQTPDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPELAHLSPHKLRHTYATIARQGGANMNQISSALTHSDISTTRIYVNTPDVV
                     DKTVFEAFQRGLNKCD"
     gene            complement(2810..2968)
                     /locus_tag="I0B46_002594"
     CDS             complement(2810..2968)
                     /locus_tag="I0B46_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAR2084836.1"
                     /translation="MNKKQTCEFLGIDNNTLDKWIRIYNFPVIRIDGIVRFDIEDIKD
                     FMNTFKTI"
     gene            3656..4396
                     /locus_tag="I0B46_002595"
     CDS             3656..4396
                     /locus_tag="I0B46_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAR2084837.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGETNYIESSKN"
     gene            complement(4488..5675)
                     /locus_tag="I0B46_002596"
     CDS             complement(4488..5675)
                     /locus_tag="I0B46_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAR2084838.1"
                     /translation="MYFLGIDIGKRTHVASIMNEEGKVLLKGFSFPNTTEGAESLIER
                     MVDYSGAPSDFVIGMEATGHYWLSIFSYLHESDYLIHVVNPLQTDGWRKGTEIRKRKN
                     DIIDSVLIADLMRYGSFVETVLSDENVFSLKQLSRYRTYLVGTASDFKRKIIAVLDQV
                     FPEYATIFTKQGVFGKASKELLLAFSAPIDLENISSETLAQYLAELSRNRIKAEKADQ
                     LKQAASNSFGVKFAQEAFTFQLRSMIEQLKFIESQIKETEREIKHIMDTLNSVILTIP
                     GIGPINGATILGEIGDIQKFSNPKKLVAYAGIDASVTQSGQYEATHNVMSKRGSPYLR
                     KALFSAALVASQCDPVFSAFYQKKVSEGKHHLTALGAVSRKLCYVIHAILTKNEPYII
                     VSD"
BASE COUNT         1997 a          934 c         1013 g         2023 t
ORIGIN      
        1 ttcgctagag tctttagaat ttctgtagta aaatcgttca tatggaagtc ctcttttctg
       61 tgaatgtgtt gtggtaactc tattctacag aagggacttc cttttttcta tttacacaaa
      121 atattttaca ctctcgtcaa atataaaaac tagtacgaaa ttaatttgta aattatttaa
      181 gcttgataaa ataagcaata tcgatgatgt aaagtccaag atattaacaa gaaaagtaag
      241 cgaaccagta aagctaacgg cacaagataa aaaaaatgga attgttcttt atggtgatga
      301 tgcaaagtat aactataatt gggatacctc ctcagaagct gaggtattaa gtattcctac
      361 taccgatact tcaggggaaa aagtcacctt taaaataaat tttataacgg caatttctaa
      421 gctggagtat gggaagcaaa aaaagagaga taacaatgat caatatctac ctaaaaaata
      481 acgaattaat gatgcatatg ttaaagttgt ttttttgaat taaacaactc aatttattca
      541 gttatatgtt catcgcagca agtgaacatt aatcgagtta taaaattaat aggagctaaa
      601 aatataaata aagttcgatc agaatttatt tttccagaag atttttttaa ctggtttttt
      661 tatcaattca gtgaatgcaa gcgactctta ggtgagaatt attacttaaa aggaatcggc
      721 ggatttatgg ggaatgtggc tgatgaacaa aatattataa aaggaatatc tgaacaaaca
      781 tcagagctta cagtaactaa agcatttata agtaatggtg aaagtttgaa aaatgtaact
      841 gcgcgaatta gaaaagaaaa tgaagtagaa attgtttttg gaattgacca tgaatgtaat
      901 tcagtgatat atgtaacaca atcagcaaat ttaattcttt ttgagaatgg ttgcaaggac
      961 gattttatgg tggtctattt gtacgatata ctaattccaa aacttcgttc cttatatatg
     1021 tcttctgaaa gtatttttgt gagtgaacaa aagggatctt tctcgaaaaa aataggtatt
     1081 gaagtaattg aatcgattat gtgtcataat catattacgc tagaagaatt agaaattttg
     1141 atagggcaaa aaaataataa cacgaaaata caaatttctt agaaatcatt ttttattgag
     1201 atatctagat aaatgattta ttcttcaatc aaccaactcg ccaagctgag gtaatgtttt
     1261 gaggtaatga taacatacaa ctttttatac cctagtaaaa ctctataaac aaagacttat
     1321 taatttcgca tgaaatacat tcctgtaaaa tgcaataaaa tagagttttg tttaaccagg
     1381 tctcaaaaag gctcgtaaag catctcaatt ctctaaacgt taatattatt tatatatcaa
     1441 tgtttcaagg cacttttcaa ttgtttgaaa agtgtctttt tcttgtttaa gaacacattt
     1501 aagaacacat actaatcaca tttattcaat cctctctgaa aggcttcaaa aacagtttta
     1561 tctactacat ctggtgtatt tacgtatatt ctcgttgtag aaatatctga gtgtgttaaa
     1621 gcactagata tttgattcat atttgctccg ccttgtctag ctatagttgc ataggtatga
     1681 cgaagcttat gtggagataa atgagccaat tcaggatgtc gtcttttaat tgaattgatt
     1741 cgatagttta ggtaatcagc gtgaacaggg caatttactt ctccggatgg tttagtatag
     1801 gtaaagagat actgatctgg tgtttgatta atttttaatc tcaacaattg ctcagcttgc
     1861 actgatttcc atttgcttag taatgccatt acaatttcgg gtacttgaat tacggtatcc
     1921 tttcgacctt ttgtaaattt ctttttttga tatttatcca aagcatgttt aaggcgaacc
     1981 gtttgtttct caaagtcgat atatttccat tgcaaagcat acgtctcact ttttctatca
     2041 ccaagatata gggtaagcat gaaaagagta tagtcatgaa gagtaagcaa gtgattattc
     2101 aagtcagtgt ttactgcttc tatccaagca ataagctctt ctgacgataa tgcttgatta
     2161 ccttcccgta tccttctttc ttctaaagca atttttttag gtgcagtgat acattgaata
     2221 attttggtag ttcggttaaa ttcaatataa tttagtattt ctgcaatatc aaacatagaa
     2281 cggacatatc ctttgataat ttttatattt gcgtattctt tcgatttttt tgttaattca
     2341 tctgatatta tctcagtatt tgaatttaga tagttcattg catactcacc aaacattgga
     2401 aggatgtgca atcggaatat atctttagta ttactaattg ttacgtaact tggagctctt
     2461 tttgatctag tagtctgacc tagttcatat ctaggaagcc attttgattc atagaattcc
     2521 ttaaatttta tttttccttt taattctagt gaatttgcat tctcctctct taaaactgtt
     2581 tcaattttcc ttttcattgc attttcggct ttaatagcca attgcctagt tggaaatact
     2641 ttttgatact tttctgatgt aattccagtg atttctctga atttttttgg atacctcaga
     2701 ttagttttat atttcccatt tgaaagtttt gtaggcaaaa ttcctcactc ccttaaaaag
     2761 taaggcagta tatttcgcta ttcttagtat atgcggcgag aagaaatttt caaattgttt
     2821 taaatgtatt cataaaatct ttgatatcct ctatgtcaaa gcgaactata ccgtcaattc
     2881 tgattactgg aaaattatat atacgaatcc acttatctaa agtattatta tcaattccta
     2941 gaaactcaca ggtttgtttt ttattcaagt agactctcaa ttttataaaa tcatctccta
     3001 aaaaagatta gaatttttga attgctttgg gtaagcacat aggaatttca cctctcccca
     3061 gtttacggcg agccttacgg aagtatcatt atttgctgct attgtcatgg cattttgttt
     3121 tcccagaaca aaaagaaatc tctaagtatt tgtcgctaat caggtcatgg cgtacttgag
     3181 tttctagctc gaatagcagg gatcgttatt caaaaattgt atttaattgt caatgatcaa
     3241 aaataggttt tttcctattc actattaata acgataaaca tgtataaaaa gacacactac
     3301 gaaaaaagtt aaatggagtt tactgatttt catatttgta tatggaacag acatagtctt
     3361 gaataatttt tataaatgtt ttgatgctta tcaacttatt caacccgcta tccactttaa
     3421 gtataatttt tccatacgcc aataaagtag ttttaggagc atgttctcat ttgagaacgc
     3481 tcctttttta tatattttga aagctactta ctatacgcgt agtttcaaat agttttgtca
     3541 ttctaatctt cagcaacaga aaaaaatcta aattataaaa tttagggttg actaaaatat
     3601 aatttttgta taccatatga tcgtgataat cattatcatt tagaaggaga aagttatgtt
     3661 agaagtgaaa aagcttaatg tttgttataa cgatttttta gcattgaacg agatttcttt
     3721 aaaaatacaa gaaggtgctt taacaggtat tattggtcca aatggtgctg gaaaatctac
     3781 attgcttaaa gctatgttaa atattatccc tcatcaggga gaggtctcca ttaataatga
     3841 agatatcaat aaaaaactgg tgaagattgc atatgtagag caaaaagctg atattgattt
     3901 tacttttcca ataaaagtaa aagaatgtgt atctatggga acatatgccg gaatgaaagt
     3961 ttttcaaaga attaaaaatg cagaatggca aagagtttca aaggctttgg aaaaggtcga
     4021 tatgggaaaa tattctaatc atcaaatagg agaattatct ggaggacaat ttcaacgtgt
     4081 acttttagca agatgtcttg ctcaaaatgc ggatttcata tttttagatg aaccatttgt
     4141 agggatagac ttagttagtg aacgaataat tatggatacg ttaaaagaat tgaaacatca
     4201 gggaaaaaca gtgctaattg tgcatcatga tttaagcaag gtaaagaagt attttgataa
     4261 tattattctt ttgaatcgaa atctaattgc tcacggaagt gtagaatctg tatttaatga
     4321 agagaattta aaaaaagcct atggagatac tatatttatt ggagagacta actatataga
     4381 gtcaagtaaa aattagccaa atcaaaaaat attttataac agataattcc gaaaaaaggg
     4441 tacaacaaat aagcttctta ccattagttt ttttgaatgt tcaatagcta atcagaaaca
     4501 attatataag gctcgttttt agtcaaaata gcatgaatca cgtaacaaag cttacgagaa
     4561 accgcaccta aggcagttag atgatgtttt ccttcggaaa cttttttctg gtaaaacgca
     4621 ctaaaaacag gatcacattg agaagctact aaagccgccg aaaacaaggc ttttcgcaaa
     4681 tatggagacc ctcgtttact cataacatta tgcgttgcct catattgccc tgattgtgtc
     4741 acggaagcgt ctatcccagc gtaagcaacg agttttttcg ggttagaaaa tttttgaata
     4801 tctccgatct caccaagaat agtcgcaccg tttataggac caattccagg aattgtgaga
     4861 ataacagaat ttaaagtgtc cattatgtgc ttgatttctc tttccgtttc ctttatttgg
     4921 ctttcaatga attttaattg ttcaatcatt gaacgtagtt ggaaggtgaa cgcttcttga
     4981 gcaaatttta ctccaaagga gttagaagct gcttgcttca attggtccgc tttttctgcc
     5041 ttgattcggt tcctgcttaa ctcagctaag tattgggcca aagtttctga ggaaatgttt
     5101 tctaagtcga tcggggcact aaacgccagc aataactctt tagaagcttt tccgaaaacg
     5161 ccctgcttgg taaaaatcgt cgcgtattct ggaaagactt gatccaatac ggctataatt
     5221 tttcttttga agtcgctcgc tgtacctact aaataagttc ggtaacgaga aagttgtttc
     5281 aaagaaaaaa cattttcatc ggataagacc gtttcgacaa atgagccata gcgcattaag
     5341 tcagcaatca aaacagaatc aataatatcg ttttttcgtt tccgtatttc tgtaccttta
     5401 cgccatccgt ctgtttgaag gggattcaca acgtgaatca aataatctga ttcgtgaagg
     5461 tatgaaaaga tggacagcca atagtgcccc gtggcttcca tccctataac aaagtcactg
     5521 ggtgctccgg aatagtcgac cattcgctca attaatgact ctgctccttc agtggtgtta
     5581 ggaaatgaaa aaccttttag taataccttt ccttcctcgt tcatgataga agcaacgtga
     5641 gtgcgtttac cgatatcaat gcctaaaaag tacatgtata tctgaccttt caaaaaaaga
     5701 attttggatt gaaatccact caactgaaaa gcgtcactac cttgtacgag atacggagta
     5761 aagtaaggac tttcaacatc taactcatac gcaatccact tatcagagag aggagccagt
     5821 ctctcgagtg cgggttcaat ggcccaaggg gggaacggct tagcctctca atccaataaa
     5881 tatattatct caaaagaaat gagttaatac caaattaaaa taacgatggt tcatagggtt
     5941 atccaatcaa aaccggaagg ttaaaac
//
LOCUS       DACKAC010000105         5905 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.107, whole genome shotgun sequence.
ACCESSION   DACKAC010000105 DACKAC010000000
VERSION     DACKAC010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.107"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(95..667)
                     /locus_tag="I0B46_002597"
     CDS             complement(95..667)
                     /locus_tag="I0B46_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAR2084839.1"
                     /translation="MTNKDQNKNYLEAIILELQNNSKRINSVELEQFSEKIVQAKHIF
                     LAGAGRSGIAMQAFTNRLMHLGFSVSLVGEISSPHSQPGDLLIICSGSGETGSLKNLA
                     AKANQSGVSIALVTMKKDSSIGQLADSVLVLPGITKDENQREEDTFSQPMGSSFEQLA
                     FLTFDSLVLSLMDKTGESSETMFARHADFE"
     gene            complement(674..1336)
                     /gene="rpe"
                     /locus_tag="I0B46_002598"
     CDS             complement(674..1336)
                     /gene="rpe"
                     /locus_tag="I0B46_002598"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAR2084840.1"
                     /translation="MTIAPSLLNAPIAEVKELFEILKEKNVDYLHIDVMDGCFVPDMA
                     FGPSVIKDIRPLTDVVFDVHLMIDTPEKIVTRFVEAGADIVTFHIEATKHAMKVIQLV
                     KEAGAKVGITFNPATPVSFIEPVLSLVDQVLVMTINPGTENKHFIQETVVKIEQLDVI
                     RKQNDYTYDIEVDGKIDNQTIKVCSKAGADIFVSGGFIFNGKNTPQEQIDKLNQSLEE
                     VK"
     gene            complement(1352..1867)
                     /locus_tag="I0B46_002599"
     CDS             complement(1352..1867)
                     /locus_tag="I0B46_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HAR2084841.1"
                     /translation="MRFHHDTLTIETVSGRPSYHDIRKELLDTVDTAGIENGMLVISS
                     PHTTCSLFFEETMHDTNFFGDDYLHVDINNVMETIIPKMTSENQYNSPGPKHIDFGTR
                     LSDPNYPAEKWVMLNTDAHLRSSIYGTNSMTLIVQEGKLLLGEMGRVYFVDWDQLRER
                     TRTINLMIMGE"
     gene            complement(1869..2153)
                     /locus_tag="I0B46_002600"
     CDS             complement(1869..2153)
                     /locus_tag="I0B46_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAR2084842.1"
                     /translation="MKKLLVICGAGHATSTIAVAKLSKWLEEEGLTDQVKIYQSKIAD
                     ELNKIDEYDAVVSTTIVPDVIKAKVTNGLGLLTGIGTDAIYADVKERLGV"
     gene            complement(2187..3515)
                     /locus_tag="I0B46_002601"
     CDS             complement(2187..3515)
                     /locus_tag="I0B46_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAR2084843.1"
                     /translation="MDIVLNAFEWFIGLGSNVFLPLIIFVIGILFGLKPGKAFVSGVT
                     VGIGSIGLGLVLSLLSDGLGTAIQQMGEQYGGGLNILDIGVGVGGPLAYSTSLGILMI
                     PISLILNFIFVVLGWTKTLNVDIWNFWFAIFLGMITQSVTGNFWFGLIGAIVAVILQW
                     FLADRTQKTVSEFFGYPGISITHMMGLSGVLFAKPVNWVFDRIPGFNKIDADAETLTK
                     RFGVLGDTVVIGLIIGIVVGIMAGYDVAGIGNLGIMTAAVMKIMPKMVAMFMEGLMPI
                     AEAAKEFTNKKLNGRQVNIGMDAALTVGHPTIMSTSLLLVPISLLLALILPGNKVLPF
                     GDLAFFAFAICLMIPYFKGNIVRSLVGCSLYVVTTLYMATWLAPIITDVFTLASFDVG
                     TAGLVTMLLCGLWPVGLFVLLASYFGVIGIGGLGVVLVGASIYMNKVKKA"
     gene            complement(3529..3984)
                     /locus_tag="I0B46_002602"
     CDS             complement(3529..3984)
                     /locus_tag="I0B46_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAR2084844.1"
                     /translation="MYLDKELCLFQLEVKDQKELFQVMSEQLKNAGCVKDSFLEGITN
                     REQEFPTGLEVNQIGFAIPHTDSAHVNSSQICFASLKEPLVFSDMTDKSHEIPVRLVF
                     MLAMSQPHEQIDTLQNLVSLFQNEEKVNELLACTTKEAFIELVHSAGVY"
     gene            complement(3987..5852)
                     /locus_tag="I0B46_002603"
     CDS             complement(3987..5852)
                     /locus_tag="I0B46_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAR2084845.1"
                     /translation="MAVSTKTIYQDIELLEDYVVNYQVTIERLPSLGIKLIGDRKNVE
                     HLISSVENYLEKDIYSPEKRRVEIIKEVLFGDKPVSLTHLSQSFMVSKTSLYNDLRII
                     NKIIGSGEVKLQSSAEGISFTGNEQAIQRAIKQLVFYYANDMESKTFFQVLNMLFDSK
                     VISVIHDLLFNDYSELTEKVSDYYVRSLISTLVIQCNRLLKGHHLTVEEEFLFNSIRY
                     METYIVANGLIEELKAKLNLPFYDADKEYLCRQLFAHRVTNNLKTSNEEYAMSVHKLI
                     ERMSEIEKIDLTQNDRLYQSLLYHVPAMVLRLKKGIKIQNPLLENIKSQYSELFSIVW
                     YAMSILEEEFDVTLTDDEVSLILIHFQIAIEDQSKANNIIIICQYGISSAQLIYNKVR
                     RFLPAKDNVEISTLDKFHLAEIGEVDLIISSLSFDAGGIPLVRVSPLVSKTDYMNIME
                     AYTKYLIEEPSRFIQNELIPKAVKVPTISKFVQSSLVKTGVQVESKEECLDLMISELE
                     ANSLVTEEFRASIFSREKMGNTNLESGVALPHANPSMVIESNIFIVALKNAINWGDRK
                     VKLVIMVSLAEEDVSEIRGVYEELYQFIGQKEMVDRLLQVHDSEKLLRIFQQKGE"
BASE COUNT         1919 a         1147 c          836 g         2003 t
ORIGIN      
        1 aattattaag ttatgtctaa tcaaataaaa aaaccattct catatttgct gaataattag
       61 catttagaga atggtttttt atctttaaaa tctactactc aaaatctgca tgtcgagcaa
      121 acatcgtttc tgagctttca cctgttttat ccattaaaga taacactaaa ctatcaaagg
      181 tgagaaatgc taattgttca aaacttgaac ccataggttg tgaaaaagta tcttcttctc
      241 gttgattttc atctttggta atacctggta atacaagaac agaatctgct aattgaccaa
      301 tcgacgagtc ttttttcatg gtaactaaag caatgcttac accactttga tttgctttgg
      361 ccgctaaatt ttttaaacta cctgtttcac ctgagccaga acagataatt aataaatctc
      421 caggttgcga atgtggtgaa ctaatttcac cgacaaggct gactgaaaaa cctaaatgca
      481 ttaagcgatt cgtaaatgct tgcatggcaa ttccagagcg tcctgcacca gctagaaaga
      541 tgtgtttagc ttgaacaatt ttctcactaa attgttctaa ctctacagaa ttaatacgtt
      601 ttgagttatt ttgtaattct aagataatcg cttctaaata gtttttattt tggtctttat
      661 tggtcatgat cttttacttc acttcctcta atgattgatt tagtttatca atttgttctt
      721 gcggggtatt tttcccattg aagatgaaac cgcctgatac aaaaatatcc gcccctgctt
      781 tagaacaaac tttaatcgtt tgattatcaa tcttgccatc tacttcaata tcatacgtat
      841 aatcattttg tttgcgtatt acatctaact gttcgatttt aactactgtc tcttgaataa
      901 aatgcttatt ctctgttcct ggattgattg tcattactaa tacttgatcg actaaagata
      961 atactggttc aataaaactg actggtgttg caggattaaa agtaatccct acttttgctc
     1021 ctgcttcctt gactaactga atgaccttca ttgcgtgttt ggtagcttca atgtggaagg
     1081 taacaatatc tgctcctgct tctacaaatc ttgtgacaat tttttctggt gtatcaatca
     1141 tcaaatgaac atcaaacaca acgtctgtta gtggacgaat atctttaata acacttggtc
     1201 caaaagccat atctggtaca aaacagccat ccattacatc aatatgcaaa taatcaacat
     1261 tcttttcttt taagatttca aataactctt taacttctgc gataggtgca tttaacaatg
     1321 atggagcaat tgtcattctc tattcccccg tttattctcc cataatcatg agattgattg
     1381 ttctagttcg ctctcttagt tgatcccaat cgacaaaata gacacggccc atttctccta
     1441 ataaaagttt accttcttga acaattaaag tcattgagtt ggttccataa atagatgaac
     1501 gtaaatgtgc atcggtattt agcatgaccc atttttctgc agggtaattg ggatcagata
     1561 atcttgttcc aaaatcaatg tgttttggcc ccggactgtt gtattgattt tcactcgtca
     1621 tttttggaat aatcgtttcc attacattgt tgatgtctac atgtaaataa tcatcaccaa
     1681 aaaagtttgt gtcatgcatc gtttcttcaa aaaagagtga gcaagttgta tgcgggctcg
     1741 aaattactaa cattccattc tcaatacctg ctgtatcaac tgtatctaaa agttcttttc
     1801 gaatatcatg atacgaagga cgccctgaaa ctgtttcaat agtcaaagtg tcatgatgaa
     1861 aacgcatatt aaacacctaa tctttctttt acatctgcat aaatggcatc tgtaccaata
     1921 cctgttaata agcccaagcc attcgttact ttagctttaa ttacatcagg gacaattgtt
     1981 gtacttacta cagcatcata ttcatcaatt ttatttagct catcagcaat tttagattga
     2041 taaattttca cttgatctgt taatccttct tcttccaacc attttgataa ttttgctact
     2101 gcaattgtag aagtagcatg acctgctcca caaataacta ataatttttt catattgatc
     2161 tcttctttct tattttaatt ttatgattaa gctttcttca ctttgttcat gtagatagaa
     2221 gcacctacta aaacaactcc taatccacct attccaatta caccaaagta agatgctaat
     2281 aaaacaaaca accctactgg ccataatcca cataacaaca tagttactaa tcctgctgtc
     2341 cccacatcga aacttgctaa tgtaaacaca tcagtgataa ttggtgccaa ccatgttgcc
     2401 atatataatg ttgtaacaac atacaacgaa catcctacta atgatctcac aatatttcct
     2461 ttaaaataag gaatcattaa acagatggcg aaagcaaaga atgctaaatc tccaaaaggt
     2521 aatactttat ttcctggaag aattagtgct agcaataatg agattggtac aagcaataga
     2581 gaagttgaca taatagttgg atgacccaca gttaacgctg catccattcc aatgtttact
     2641 tggcgaccat ttaacttttt attcgtaaat tctttcgccg cttctgcaat tggcattaaa
     2701 ccttccataa acattgctac cattttaggc ataatcttca tcactgccgc agtcataatt
     2761 cctaagtttc caattcctgc tacgtcatag cctgccataa tacccactac aataccgata
     2821 attaggccaa taacaactgt gtcacctaaa acaccgaaac gttttgttaa tgtttctgca
     2881 tctgcatcta ttttattaaa tccaggaata cgatcaaaaa cccaatttac tggtttggca
     2941 aatagtactc ctgataatcc catcatatgt gtgatagaga ttcctggata gccgaaaaat
     3001 tcgctaacag ttttttgtgt acgatctgct aagaaccatt gtaaaattac tgcgacaatc
     3061 gcgccaatta agccaaacca aaagtttccc gtaacagatt gagtaatcat acctaggaaa
     3121 attgcgaacc aaaagttcca aatatcaaca ttcaatgttt ttgtccaacc caaaaccaca
     3181 aatatgaagt taagaattaa tgaaattgga atcattaaaa ttcccaatga tgtagagtaa
     3241 gccaaaggtc caccaacacc tacgccaata tctaggatat tcaggccacc accgtattgt
     3301 tcccccattt gttgaattgc tgttcctaaa ccatctgata ataaacttaa aactaatcct
     3361 aaaccaatag aaccgatacc aacagtaacg ccggaaacaa atgctttacc aggttttaat
     3421 ccaaataata tcccgattac aaaaataatc agtggtaaaa agacgtttga ccctaatcca
     3481 ataaaccatt caaaggcatt taatacaata tccatctctc tattcctcct aataaactcc
     3541 tgcagaatgt actaactcaa taaatgcttc tttcgtggta catgcaagta attcatttac
     3601 tttttcttca ttttgaaata ggctaactaa gttttgtagt gtatcaattt gttcatgagg
     3661 ttgtgacatt gccaacataa agactaaacg tacaggtatt tcatgagatt tatctgtcat
     3721 atctgaaaat accagtggtt cctttaatga ggcaaaacag atttgagagc tatttacatg
     3781 tgcggaatct gtatgtggga tagcaaaccc aatttgattt acttctaatc cagttggaaa
     3841 ttcttgttca cggttggtga taccttctaa gaaactatct ttcacgcaac cagcgttttt
     3901 taattgttca gacattactt gaaacaattc tttttgatct ttcacttcta attgaaataa
     3961 acataattct ttatctagat acatttttat tctccttttt gttgaaaaat tcttagtaat
     4021 ttttcgctat catgaacttg taacaagcga tcgaccattt ctttttgccc aataaattgg
     4081 taaagttctt catatactcc tctaatctca gatacatctt cttcagctaa actaaccata
     4141 atgactaatt tgacttttcg atcgccccaa ttaatcgcat tttttaatgc tacaataaaa
     4201 atgttggatt caatgaccat tgatggattc gcatgcggta aagcaacacc actttctaaa
     4261 ttagtattcc ccattttctc tctagagaag atagaagcac gaaactcttc agtaactaat
     4321 gaattggctt ctagttcgga aatcatcaaa tccaaacatt cttctttgct ttccacctgt
     4381 actcctgttt ttactaaaga tgattgaaca aatttagata tggtgggaac tttaaccgct
     4441 ttaggaatca gttcattttg aataaatcgc gaaggttctt ctattaaata tttagtataa
     4501 gcttccataa tattcatata atctgtttta cttaccaatg gagataccct tactaagggt
     4561 ataccacctg catcaaagga aagagaggaa ataatcaaat caacttcccc aatttcagct
     4621 aaatgaaact tatctaaagt tgatatttct acattatctt ttgctggtaa aaaccgtcga
     4681 actttattgt aaattaattg cgcagaagaa ataccgtatt ggcaaataat gatgatatta
     4741 tttgctttgg actgatcttc aatagcaatc tgaaaatgaa tcaaaattaa ggaaacttca
     4801 tcgtccgtta aagtcacatc aaattcttct tcaaggatgg acatggcgta ccatactatg
     4861 ctaaagagtt ctgaatattg agatttgata ttttctaata acggattttg aattttaata
     4921 ccttttttta aacgaaggac cattgctggc acatgataca ataaggattg atataatcga
     4981 tcattttgag tcaaatcaat tttttcaatc tcactcattc tttcaatcaa tttatgaacg
     5041 ctcatagcgt actcttcatt gctggttttt aaattatttg tcacacgatg tgcaaatagt
     5101 tgacgacata gatattcttt atctgcgtca taaaatggaa gattcaattt tgccttcaat
     5161 tcttcaatta aaccatttgc cacaatgtac gtttccatat agcgaatgct attgaagagg
     5221 aattcttctt caacagttaa atgatgacct ttcagtaaac ggttacattg gataactaaa
     5281 gtagaaatca atgaccgaac atagtaatca gaaacctttt cagtcagttc actgtaatca
     5341 ttgaatagca aatcatgaat gacactaata acttttgaat caaacaacat atttaaaact
     5401 tgaaaaaatg tttttgattc catgtcattc gcataataga atacaagttg cttgattgct
     5461 cgttgaattg cttgttcatt accagtaaaa ctaattcctt ctgcagatga ctgcaactta
     5521 acttctccac tgccaataat tttattgata attctcaggt cattgtataa cgatgtttta
     5581 ctaaccataa aactttgaga caaatgagtt aaagaaacag gcttatcacc aaacaaaact
     5641 tctttaatga tctcgacacg tcttttttct ggagaataaa tatctttttc aagataattt
     5701 tctaccgatg aaatcaaatg ttcaacgttt ttcctatcac ctatgagttt gatacccaaa
     5761 gaaggtaatc gctcaattgt tacttggtaa ttaactacat aatcttccaa caattcaata
     5821 tcttgataaa tggtcttggt cgacactgcc aatttatcac taaaaaaaga agcgggttta
     5881 aagtcttctt gtcgacttaa taatt
//
LOCUS       DACKAC010000106         5769 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.109, whole genome shotgun sequence.
ACCESSION   DACKAC010000106 DACKAC010000000
VERSION     DACKAC010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.109"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(1014..1304)
                     /locus_tag="I0B46_002604"
     CDS             complement(1014..1304)
                     /locus_tag="I0B46_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084846.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1646..1942
                     /locus_tag="I0B46_002605"
     CDS             1646..1942
                     /locus_tag="I0B46_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084847.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1949..3901
                     /locus_tag="I0B46_002606"
     CDS             1949..3901
                     /locus_tag="I0B46_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAR2084848.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="I0B46_002607"
     CDS             complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="I0B46_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAR2084849.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(4797..5489)
                     /locus_tag="I0B46_002608"
     CDS             complement(4797..5489)
                     /locus_tag="I0B46_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAR2084850.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         1894 a          996 c          917 g         1962 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattta agtagttcag ttttggagta caaaaagtga
     1501 aaatgtaaac ctaaatgagt atcaaattgc aactgaattt gatactcgtt ttttattgtt
     1561 aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag ttaaaatgta tataaaaatg
     1621 cgaacgtgaa ataattattg taggaatgca aattggtgtg ttaaaatggt tacaatttac
     1681 taagcaaaaa ggggtgcatt ttcctatgca atttcttgag ccaaaaaata agaacgctaa
     1741 gtctgtggat tgggaaatat cagagcaagt aagggtgata gttaagcaat acgctgaata
     1801 tgcagagcgt actgaaagcg aagcagtgga tgaattctta ttgaacattt tagacgataa
     1861 gaagtttatt gaatggattg ccaacaagcg aagtaataag cggattgttg aaaaaatggg
     1921 tatcaaagat agagtgggtt gatagacaat gaccaagctt aaaagattag ccacgaagga
     1981 agatgaaatt gtagatgtta aaattccgat ttcaaacgaa gaattaaaag atagggctaa
     2041 acaatatgat ttattaactc caaaacgatt tgcaacaagg tataacaaaa tgttgttctt
     2101 gccaacgtct tttaaatgga acggttctga atatcccatt caatataatt actgcatcaa
     2161 tccgttttgt tgtaattttg ggaaagaaca gcacaaattc aaagatgtaa aagggaaacc
     2221 aagtcgatat aaaatgactg gtagttccaa agataaaggt cataaaggta tgtactgcaa
     2281 tgacaatcct attggcagag gtgtatcgca aaactgtaca gtaacccctt tatctaattg
     2341 gtctgttgtt gaagaaatta aacgccttat agaaatcaat agcatccaag atgttgagcc
     2401 agattatcaa tttcataaag aaggctgctc ggaagaagaa tcaaccccat tcaatgagcc
     2461 gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct caacgctatc aatgtaaggc
     2521 gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa accactactt atcaccaaca
     2581 aaagaacacc attcttccaa tgttcgctaa gatggttgtt ggaagggttt cagtcagtcg
     2641 tacttgtgat attttaggaa ttggagttgg aacctactat cataagctgg agtggctgta
     2701 taggcgttgt ttggagtttt tagaacgata tgagacacaa ccccttcaaa caaagaaatt
     2761 taacgagatg tggcttaata cggacaagat gcactactac ctaaataacg tccgtaagaa
     2821 aggacaaggt tcaaagaaat atacggggtt tgaagacctt aatatgcaaa cgtatatagt
     2881 tgtatctgct gaggtcttat caagatatgt gttccgttct gatgttgcct atgattggaa
     2941 tatttcaatg gatgaattaa atgaagatac aagaaaattc aaagaagacc atctcaatac
     3001 ttttagtcgt aaaaatgata ggttagattg gtcttattat ccacaagaac catccgctaa
     3061 cgatagcgaa aataggaatg cttatcttca cgaattaggg aaaattacta ataggagtag
     3121 atttgtcgat ggtttaaatg ttgatgctcc ttatactaca actgctcact attggttaat
     3181 taaacaaatg gttaatgccg acgaatggcg aatgattagt gatgatgact tctctattag
     3241 aaacgccttt tatagagtat tcacgaaaga attaaggttg agcgatgcac atcattttat
     3301 ttgccaagtt aacaagacga aaagcagaaa acaatgctta aaagaattcg gacaagcaaa
     3361 agcagaactg ctcgattggg gagatatcag aggattcaaa acaaagtttc tacggacttt
     3421 ggcatctcat tatctaactg aattactaac aagtcatcaa ttccacgaag aggctattag
     3481 taaagatgga gaaagatatc gcaaatatgc cgataaccct atcaaacatc cattagccac
     3541 taaggataaa ggattttatt cagttgattg tagaacggat ttatctgctc tggaaccaaa
     3601 cgaaatcgca aagatgctat taaatgttaa tgaccattcg accaatagct ttattcaaca
     3661 gataagacgt tatatatcat ctcttgaaag accattaaca acagctcgtg gtgataagaa
     3721 gagctatatt tacgctaact tcaatccgaa gtatgctcaa tttgcgatta caattctaag
     3781 aacttactac aacttttgtc gcccttttaa atcagcggat aaaaaggttt tgacacctgc
     3841 tcaaagatta gggattacag ataaacaatt tgattggaaa gatataattt atttcaaatg
     3901 acaaaaagag cttggagtaa tcccaagctc ttgtgcttta tctttactgt tcttcttcaa
     3961 ttaacgcacc cgttaactca ataagttttt tctttcatat acatgaaaat agtagttata
     4021 cggattttta tcattcttta tccctttttg ggcaaatacc tcattccatt cctcataatt
     4081 cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt atttttgtga tgtacatttt
     4141 ctcaacataa gggaaaaaca aattataaat ctgttctcct ccgaaaataa aaatttcttc
     4201 ttcgttttta cataactcaa aaacatcttc tattgaatga acaatttcac aaccattaaa
     4261 ggtaaacccc ttatctctcg tcagaataat atttcttctg tcaggtaagg ctcttccgat
     4321 tgattcaagg ttcttcctac ctaatattat cggatgtccc tttgtagtat ttttaacata
     4381 ttcccagtcc ttgggaatcc tccaaggaat gtcattctct ttgccaatca ctctattctt
     4441 atccatcgca gcaatcaaag aaactttcat ttataacaca gcctttttaa ctaatttacc
     4501 atctaatttc ttatttctat caaaaaacct tttctcatta tcctgcccgt tagttgaata
     4561 agcaattttt tctataagtt atattatcat aataccacaa aatcacaaat tttcattcaa
     4621 ttatcatttt caattgcatt ttataaatca ttttgtatgt aatttggtat gtaagttaaa
     4681 atgaaaaaag tagtccattt tgaagtacat tttgtcattt tgtactccaa aactgaacta
     4741 cttagtatag attactgttg cttctttact aatttaggtt tacttgttaa gaaagattat
     4801 ttacgcaaat gttcttcaac aatttgctga gatttatcag ctgtatcttc cacttcatct
     4861 tttgcttttt ctaacttttg aatatattgt ttcaagcttg agttttcagc agttgcttcg
     4921 tctaatttct tttgtgtatc tgttaactga gtttctacat ttgttttttc ttgtgtcagt
     4981 gttgcgattt gttgattcaa accatttaca gtattttgta attctgaaag ttttgcttgt
     5041 gcttcttgta gtcttgctgt ccaatctgca ttcagatgat tgatttcatc atttttttgg
     5101 ttgatctcat ctagtttgac attgatttca ttttgtttgc tttgtagttg tgcatccttg
     5161 ctgactaatt gactttctaa gttttgtttt tctgctgccg attgattttt ttggttttct
     5221 aattcattta cttttgtttg gtattcttgg acatctgcct ttgaatcttg caatgtctgt
     5281 tctttttgtg ctagtcgagc ggtaagatct gtaatcgctt gctctttatc tgtcaatgta
     5341 tgatcaagct gtgtcagatt ctcattgatt gtatcaatat ctgtctctcc accccacaaa
     5401 ataatttcat ttgcgtaccg cgtgcctgtg tagccaatta gacatagaaa tgcaacgaca
     5461 atcccaccag taataaattt ctttttcatt tgcatagctc cttaatccaa tacttttaca
     5521 tgctaaaagt aagtgatctc aaacacaatg tcaagcgttt ttaaattaca tatgtaattt
     5581 taatataaat actatcaaat actcatttaa tcctcctttt ttatctattt aaaaaatctt
     5641 ttttatagag aaaaatgcag gttaatactt tcttaaataa atatttatgg tgaacaaaaa
     5701 caaaaaagca agttttatta aacgtaaccc ttttcatttc atagattact gtatttatta
     5761 caaaaaacg
//
LOCUS       DACKAC010000107         5727 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.110, whole genome shotgun sequence.
ACCESSION   DACKAC010000107 DACKAC010000000
VERSION     DACKAC010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.110"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            196..921
                     /locus_tag="I0B46_002609"
     CDS             196..921
                     /locus_tag="I0B46_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084851.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHSQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            911..1420
                     /locus_tag="I0B46_002610"
     CDS             911..1420
                     /locus_tag="I0B46_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084852.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(1490..2938)
                     /locus_tag="I0B46_002611"
     CDS             complement(1490..2938)
                     /locus_tag="I0B46_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAR2084853.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(2938..3654)
                     /locus_tag="I0B46_002612"
     CDS             complement(2938..3654)
                     /locus_tag="I0B46_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAR2084854.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            complement(3635..3985)
                     /locus_tag="I0B46_002613"
     CDS             complement(3635..3985)
                     /locus_tag="I0B46_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAR2084855.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            4129..4902
                     /locus_tag="I0B46_002614"
     CDS             4129..4902
                     /locus_tag="I0B46_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAR2084856.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMDNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            5073..5439
                     /gene="ssrA"
                     /locus_tag="I0B46_002615"
     tmRNA           5073..5439
                     /gene="ssrA"
                     /locus_tag="I0B46_002615"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         1662 a         1124 c          994 g         1947 t
ORIGIN      
        1 aaaattattg tcgctgctcc tcttttcgaa taaacagtgt tcaaaagctt gcatctacgg
       61 taatagaata gggcagcttc ttcacaacct cttgcttttt acaaaatatt ttctggtttt
      121 aattcgtgat ttcactaaag gaatttcata ctttattcat attcattttc tatacttttt
      181 atagaggtga aaagtatgaa aataatccgc tatgctactg ctcgattaat ttattacaaa
      241 aagcagacaa tactctattg tttattttca agcttttctg cttttttatt gatagcatgc
      301 ctgactcttt accattcgca atcggcttta gccaatcaag tccaagatcg gctttcattt
      361 attggcgctt ctgcatctac agagcttctt ccggctttag aattagctag ccgattttat
      421 ataaaaggca tattcagttt attttttcta tttgccttcc tttttatctt tttcttttac
      481 tattcagtca ggcaaaacaa gcaggaattg atcaattggc gcctgactgg tctttctaaa
      541 accaaattat tcctttttat tttctggcag ctgttctttc ctctctttct ttgttgtatc
      601 ttggttttat tgtggatcgt cagcttccag tcagtttatg aagcgcttct tcaaaggatc
      661 aattttcttg tcttaaagac tactgaactg ccagatatcc gtgatatctt gacttcggta
      721 agcggactta caattccgat ggatcaacag acttttttta ccatcgattt ccaaaatgat
      781 ctttttttca cagatatact taagggattt gcccaaactc ttattgggct cagtggatgc
      841 tgtctcattc ttagtctatt ccctttcact ttttttatcc atcatttgca aagtaggagg
      901 cctggacgta atgaaacctg aatatttgat tcatgctgaa cacttgaccg ttattttgtg
      961 tcctctcgaa aaacagcata aaatattgga gcacttgaaa aaagaggttc ttgttgcaaa
     1021 actggataat tttgcgatca tccaaaaaaa ttggccgatg tttccttatc tgaatcttaa
     1081 agaccatgtc ctgttggatg taccggaaaa agaaatcaaa caagatcgtt tggcctatca
     1141 agaaaaactt gatatctctc cttccttgct taattgtgcg gcagctgagt tgacgtcttt
     1201 tgaaaaagta aagctgcaat tacttcatgc cttgctttcc aagcgaaaca atattgttat
     1261 tgaagataca tttgatgaac ttagtgtatt ggagatccaa gagctactcc acttgctttc
     1321 ttatttagct catgaagaaa atcaaggtat cttactcttt actcatgatg ctactattgc
     1381 tcaaagtcct tatattgatc gtttagagcc cgcgggctaa atcaatcatt ttatagtaaa
     1441 aacaagcggt atagaagatc gattcatgat cttttatacc gcttgttttt tactcttttt
     1501 tgatttctac gtcaggcaaa cgaacagcga attttgttcc ttttcctagt tcgctttcta
     1561 cttctaattt tcctttatgc aagcgtacaa gttgctgcac gattggtaaa cctaatcctg
     1621 attcaccata ttttgtattt tttcttgaag gatctacttt ataataacga tcccagatac
     1681 ttttcatttg ttgttcagac attccaattc ccgtatcttc aatcgtgatg atcgtttcaa
     1741 gatagccttt ttctaaagta atcgtgattt ggccgttttc agtaaactgt atcgcgtttt
     1801 gaataatatt gaccatgatt tggacaaatc ggtcataatc agcgtaaaca tcaatatcat
     1861 caacggcttt caatgtcaat gtatctcctg ctgcttctgc tttagcttct aactgtgcaa
     1921 caatattttt caatgtttct gttccattga attttttaat gaccatactg atttgattcg
     1981 tacggatttt ttcataatcc aagttttcat tcaccaaacg gatcagacgc tctgtttcat
     2041 ttttcatcaa tttaatcgcg ttttctcgct gattttcagg gattgcattg tattctaacc
     2101 cttccagaag tccgttgatt gtcgtaagag gtgttctcat ctcgtgagaa gcatctgcca
     2161 taaattgtct tcgacgattt tcttgttctt caatttctgc ttgagattct ttcaatgaat
     2221 tcgtcatttt attgaaatca tctgctaact catcaaattc atctttgtca tgtacgggga
     2281 gctgtacatc aaaattacca ttcgtcactt ctttcgtcgc attccttaac cgattgatac
     2341 gcttaacttg gaatgctgca aaagcatagc tggcaataac tgcaacgata ctggaaaaaa
     2401 taaatccttt gaacaggttc agtgtaattg gacgaacgct gttatcgata tttcgcgcag
     2461 gttgactaac tacaagtgct ccatagaatt catgattcaa attaaatggc acaagtgcgt
     2521 aagaggtcgc ctgtgcttgt cctaaaatat ttttattcga tgtgaatttt tcttgacgtc
     2581 cattcctcag atttttccat tgatcagctg ttaaggtaaa attcagtaaa gctgtattag
     2641 tcggataaat gacacgttcg tttttatcga taaatataaa gttcacattt tgttctgtca
     2701 gtacttgctc tgtcagcagc aatgcatttt gaaacgcctg atcttgacta aactgcggga
     2761 gcgtatcggc ataggtttgc gttgtctttt caactgattc agcatatcca aacagttgct
     2821 ggtaattgtt ttcttcaatc gtttgttttg tcagttgtgt aaaagaaact ccaacgatca
     2881 ataagatcaa cgcgaccatt ccgcaaaaag ctaagagttg ctgatacaaa taacgcatta
     2941 ggcaacacca gaatcatcga atttgtagcc gactccccaa acagtttgga tgacttgtgg
     3001 tccgaccttt tcgatttttt gtcgtaattt cttgatatgg gcgtccactg tccgttcatc
     3061 accaaagtac tgataatccc agactaattc aagcaactgc tcccgagaga atacttgacg
     3121 tggttttttt gctaaagtaa acaatagatc gaactcttta ggcgtcagtc cttcaattgg
     3181 ccggttgtcc agatacgctt ctcgtgtttt cgtattcatc ttgaaatgac tagtctcgat
     3241 atcaaaatga tcatctgaaa ctgctgcttc ttctcttgct tctgccaact cactacggcg
     3301 atgcaaagct ttcattcgag caataagcgt cagaggactg aaaggttttg tgacataatc
     3361 atctgccccc atctctaagc cgatcacctg atcgctttca gaatctctag ctgtcagcat
     3421 gataatcggc acagtacttg aaactttgcg gatctctctt cctactagca taccgtccag
     3481 tgtgggcaaa ttcaggtcta gtgtaatcat atcccaatgt tgtgggtttt ctaaaaaggc
     3541 atcgagccct tcttttccat catacttgaa agttgcttcc caaccttcat ttaagaaaaa
     3601 catctgcatc atttcagaaa cagattgatt atcttcaatc attaaaatat tcattatcgt
     3661 tccctccagt ttccaggtca tatttctgta atttcttttc tgtcataata aaaataggca
     3721 taattggaaa tactagcaga agcatttcaa tcagaaaata attcgtatgc ccagatgtct
     3781 ttaaataata gccgatcaat gccatcagta agcccataag aaaaaaccag tttccgcttg
     3841 tcttttgagc cagccgccaa tgagcgtctg tttttgcggc aagaggcgaa tgatacccat
     3901 aaaaaggcag atttcctttt gagggcagaa tccgaaaaag actgccaatt attaagaata
     3961 agataccaat actgaaaaaa atcattcttt ccctcctaaa ttctcttctt atagtatact
     4021 ctaaattgtt gactgaacca atatgttcag gaattcttca cgaaaggttt gtcttttgtt
     4081 cattctttgt taagataaac ttattccaat aaaaaaggag ttttatttgt gtcaaattat
     4141 cgtgcaatca ctttctttga tcttgatgga acattactag atgcgaaatc aaaaatcaca
     4201 cccgaagtgg caaaagctat gaacgcatta aaagaaaaca atgttctccc cgtcatcgct
     4261 acaggacgta cagaagcaga aatccactct atcatggaag atgcgggcat tacttctgcg
     4321 gtcaccatga atggttcttt cattcgagta gacggcgaag aaatctattc tcaaacattt
     4381 tcttcagaag aatgtcaaaa aatgtatgat catgtacagt tgaacggtca tgaactttct
     4441 tactataacg atcagaaaat ctggtgtacc ggtcatagcc aaacaatgat caatgcttac
     4501 gacttcattc attcagatgt tccggaaatt gatcctttag gctttaaaga aaacacagta
     4561 aatatgttgc tgatcttatc tcaatcaggt gacgaatact atcacgaaca ttttcctgag
     4621 ctaacttttt atcggaatgg tccttactcg atcgatacag tgaagaaagg aatctcaaaa
     4681 ggggcaggtg tcaaacgtct gcaaaaagaa cttcagctgg aaaaagttcc tacttttgga
     4741 tttggcgatg gtccgaatga ttttgccttg ttggaagctt gcgacaacaa aatcgctatg
     4801 gacaacgcct atgacggatt aaaagaactt tctaccttta tcacaaagaa aaatacagaa
     4861 ggcggaattg tccacgcatt gaagtatttt gatctgattt aagatctttt tttgaagcta
     4921 cctcttttct taagaaaatc gcttattttt tcttaagaaa ggggcagtta ttatataaat
     4981 aatgggatat gattcgcttt tttattgaat ttatcccaat atatgctata ctaattttgt
     5041 atgaaaaata cacgtaaccc ttgttagcta acggggacgt tacggattcg acaggcacag
     5101 tcgagcttga attgcgtttc gtaggttacg tctacgtaaa aacgttacag ttaaatataa
     5161 ctgctaaaaa cgaaaacaac tcttacgctt tagctgccta aaaacagtta gcgtagatcc
     5221 tctcggcatc gcccatgtgc tcgagtaagg gtcctaactt tagtgggata cgtttcaact
     5281 ttccgtctgt aagttgaaaa agagaacatc agactagcga tacagaatgc ctgtcactcg
     5341 gcaagctgta aagtgaatcc ttaaatgagt tgactatgaa cgtagatttt taagtggcga
     5401 tgtgtttgga cgcgggttcg actcccgccg tctccatata tgtatggttg agacagataa
     5461 acatgtatga aaaacgttgt taaatcaacg tttttttatt ttggtcaagg agagaaaagt
     5521 agagaaatac aaaatttttt gcattgattt tgcattcaaa aaaacattca aacatttaga
     5581 aatccccaat acgtattcag gaaaatctat tttaaggttt acaaaaagaa cttttgttcg
     5641 tacactcttt ttgaggtgaa cttttatgct gatggaagga aaaacacaat tatggtttaa
     5701 gcatgttcct tcaaataagt tttttta
//
LOCUS       DACKAC010000108         5339 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.112, whole genome shotgun sequence.
ACCESSION   DACKAC010000108 DACKAC010000000
VERSION     DACKAC010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.112"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(62..280)
                     /locus_tag="I0B46_002616"
                     /pseudo
     CDS             complement(62..280)
                     /locus_tag="I0B46_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(546..740)
                     /locus_tag="I0B46_002617"
     CDS             complement(546..740)
                     /locus_tag="I0B46_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084857.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            2092..3132
                     /locus_tag="I0B46_002618"
     CDS             2092..3132
                     /locus_tag="I0B46_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAR2084858.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
     gene            3956..4288
                     /locus_tag="I0B46_002619"
     CDS             3956..4288
                     /locus_tag="I0B46_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAR2084859.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            4907..5248
                     /locus_tag="I0B46_002620"
     CDS             4907..5248
                     /locus_tag="I0B46_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAR2084860.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1942 a          728 c          900 g         1769 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattgta ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aagtactacc
     1021 taatttaagt attacttaaa caatctaaaa aatagttatc ccaattgtta tgcttttata
     1081 atacgattat ttgtaacaaa atgaatatta tataattaaa cactaccata tttaggtagt
     1141 gtttaattat tagcaaaaca aaaaatattt attctattct cttaacttca actaagttat
     1201 gctgcacacc tttatataaa tcatatgcaa ctcattagct agacaaacta caagcatgta
     1261 gttttatctg ttaattatgt actacctaaa taaagtagtt tataattagc aagtatatat
     1321 atttaaaaat ttaagattaa tttttttatg acttgtacaa atttatctga tattctctta
     1381 ttaagaacta cctaatttgg gtagttctta ttttttacat tttaagtagt atttaattgc
     1441 aatttggtag tattttttta ttatcttatt cttagaacgg tagtacttaa tataaatata
     1501 ggtagtatct aatttaagta ttaatggtag tatctaaatt aacaggtagt atgtaactac
     1561 tttaaagtaa tttttttcag attaacacag tagtatctta ttaaataagt taattttaat
     1621 ggtagtacat aattattata tatatctgac attggtagta cttattgtat tttttagtag
     1681 tatataatct attgttttct agcctaaagc cttgtcttat aagaataata taatatatac
     1741 tactgaaata aggtagtata tattactagt aatggtagta tgttttatat gcataaggta
     1801 gttcttaatt tattttttgt tttgacactg gtagtactta attatcttta attttaaaac
     1861 taaattttat tcatacgttt ttctggtagt atttaattat aaataatatt tatttaaagt
     1921 agttcttaat gactttaaag gtagtacata attcatttta tttctgattt taggtagtat
     1981 ttaattaaaa aacagtttta atgcgtttgc attttttatt tatctttgct attttcgaaa
     2041 agagtaggta attttacata aataaaacaa cgtgaaagta gaggatctac aatgactgat
     2101 tttaaatttt ttaaggcgga tagagtttac aacgaattat tttatcaatt tccaaaagtc
     2161 tttattgttt ctgacgaata caaaaaaatg aaagattcaa ctaagattgc ctatatgctt
     2221 ttaaaagcaa gattagagat cgcaatcagc aaacggcaaa tcgatgaaga aggtaatgtt
     2281 tattttactt atacgacaaa tgaactatgt agagtattaa actgccaaaa acaaaaagcg
     2341 atagcaatca aaaaagagtt ggaatccttt ggtttattat tacaaaagca gatgggattt
     2401 aacaaacagt tagggaaaaa taatcctaat agactatatc tagcagaatt aaaagtctca
     2461 gaaaatgata tctacttact cgaaaaattt gatagagaga atagggaaaa cgttgataaa
     2521 tcagagggta tgaaaatcat acccaccctc gacgaaaaat cagacgctga atcccttggg
     2581 gctcaagagg gtatgaaaat cataccgtgc caaaacgttg ataaatcaga gggtatgaaa
     2641 atcataccag aacttaataa taatatatta gacactaata gacacaatat agacactgaa
     2701 aaagaccgcc tacaagatca attgttgtta gacaattttg agacaattat gacaaacgac
     2761 agcattgcta cgtttgtccc tgaacgagta ttaaatttga taaaaacatt ttcttcaagt
     2821 tacagtgaag ctcaaaaaac cgtccagact attcataatg caaagaaaaa agctgaaata
     2881 gaaagtggta tttcgatagt ttttgaagaa ctcgatagtt attatgtcaa tgcagaacaa
     2941 gaattataca cgacactgtt aaaagcctat caaaaattaa aaaccgaaaa agtcgaaaat
     3001 atccagaacc tgatttttgt ctatgtaaaa aattggttta tcgaaaaacc aatagctgct
     3061 aaagtatcaa gtgaaaaacg tttgaattat gaaagctccc caagcactat tacgaaagac
     3121 tggttagagt gaaactttac tgacaaagtg attgttgctt catggagtaa cattaaaatt
     3181 aaaaattttt tattattcta attatattac gaatattaca aaataatagc tattttaata
     3241 gaatttataa gttaaaatat tatgataaaa atcacaaacg tagcctttta cgtcataagt
     3301 gttaaaagaa ataattaatt tcaaatatag aaaagggaac gtatgtttgc atactccttt
     3361 tttttgagtt ataataatac gcgaagagag ataatttcat tatttaaaca agctaaaaat
     3421 cttgatatta cttcttttca ggcaatacga atattattcg cttacatttt ttgtataaat
     3481 ttaattgtct gctatgcttg tgatgaattg attttcgttc tgcacttttg gatgataaat
     3541 ttggacaatt gaaaatacaa aaaaaagaaa ggcggtatga actcggaagg ctggtaacct
     3601 tcgtcagcgt tcatacacgg cattaagatt tggcccttaa tgcgttaacc tttcagtgtt
     3661 acggtattac ccgcaatgga tttagtataa cagatattca aaagtgatac cacttttttt
     3721 ggctgtgtta aggttaaaaa tacgtgatat tacgatgaat ctatgcctaa gcacaatgtt
     3781 ataaaaatgt ccgtgtggct ctttgccgta tgacgaacgc tgaaattaga gggaacgcat
     3841 gtttggatgc gttccctctt ttttgtttgt catgaacgaa aatcaattct ctatttttga
     3901 aaaataacaa gaaaaacttg ttaaaaatat ttaaagaaat tgggtggttt attttatgtc
     3961 atatacagga attttgagtc tcaaagatat ttgtcactac ggtaaacggt gtaccgctac
     4021 agagaaaatc acgaagaaac tatcaactgg ccaaagtaaa accgttgtgc agtgtaagaa
     4081 atatataatc cagaaagaca aagtctcaga ggaaatgatt tattacattg ggaaacaaaa
     4141 gcaaataatt ttaaaagatc ctatcccctt aaaagagcta tatccaacaa tcaaacatgt
     4201 ttatgaccaa aacggtgtac tgattggtcg aagaaaaaat ggagtcttgc gttgtaccgc
     4261 aaaagggatg gggcgtttga tcggctgaat tgatcattct cataacttgt attttatcgt
     4321 ttttcgcttg cttgaaaata ttgtcgagac ttctaaagga acttagagtc actagaaaaa
     4381 taaggagtag acttgatcta tcaacaattc ccttcgggga gtgacggggt aggttttaac
     4441 gattggtaac gtaaaaaatt ggtcaatcga gtaaatgcgc ttgtttggat agtggtgaaa
     4501 tgaatttgtg taaataataa gcgcagagta aacaagaggg aatttagaag ttagacaagt
     4561 tagaatagcg tctagattaa tcatgagcag ctactaaggg atctagactt tgacgcaaac
     4621 tcatggaata aggagtaggt cttctcgtaa tggggaggac gcacaagaaa ttgtgtcgtg
     4681 tgctgcagta catgtagata cttgcgagga cgttgattat tggatcaatc gagcatttac
     4741 cgctgtctac tagggtttct cgtttcggac gccataaacg agtcgaaatt ttttatgtat
     4801 ttttaagtaa acgaaaaacg ataaaatatg aacaagtcct gaataaagtg aaaaaattga
     4861 tataaaacat agagaaaatg aaatttacgt aatggaggaa tgacaaatga aaaattatga
     4921 tccgaatata cgattgggca cacatactat taaggtttct ttccaacgtt gggattacaa
     4981 aggatttgtg acgtttagaa gaggcggaaa ttgtaaagga ttagatgttt tagcattaga
     5041 cgaggatgat ttatacgatc aaacattgac tgataatcca attggattcg gcttgctacc
     5101 tgaagatgat gaaggtaatg aatggtttaa aatgacgctt atgaatgata atggagacga
     5161 attatctgtt gaagatactt ggtcatattt gagtgattat attgtcagct ttgaaatcat
     5221 tgaatttgta gcggataaag aagaataaaa actggaggaa taaattttac agataaaggc
     5281 ttagaaggag tcacgatgat taataaaaag gtagtattac cttaggcatt gagtaagga
//
LOCUS       DACKAC010000109         5120 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.113, whole genome shotgun sequence.
ACCESSION   DACKAC010000109 DACKAC010000000
VERSION     DACKAC010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5120
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.113"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(207..1169)
                     /locus_tag="I0B46_002621"
     CDS             complement(207..1169)
                     /locus_tag="I0B46_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAR2084861.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="I0B46_002622"
     CDS             complement(1171..2610)
                     /locus_tag="I0B46_002622"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAR2084862.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..4741
                     /locus_tag="I0B46_002623"
     CDS             2795..4741
                     /locus_tag="I0B46_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAR2084863.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            5005..>5120
                     /locus_tag="I0B46_002624"
     CDS             5005..>5120
                     /locus_tag="I0B46_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010051189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAR2084864.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEET"
BASE COUNT         1665 a          855 c          841 g         1759 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaagc acatttaaaa ttaatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata
     4741 aatatatttc cctgagtaaa gatggaattt tctttccaaa atcaaataat atagattaat
     4801 caaatatttg agaataacat tttatttaat cttttcatat atcaattgta tgaaaaatta
     4861 ttagaatttt aaaaaatgat aaaaattcaa aaagaaaaaa tagtagttat ttacttgtca
     4921 tgttactaaa taaaaaaata atttaaatat gatatatagt catttattta gtgatttcat
     4981 tctaaaataa agggagaaat gtctatgcta tacggtgcaa ttgaagcagg cggcacaaaa
     5041 tttgtgtgtg ccattggcga tgaagaaatg acgatcaagg aaagagtgag ctttccgaca
     5101 acgacacctg aggaaacaat
//
LOCUS       DACKAC010000110         4809 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.114, whole genome shotgun sequence.
ACCESSION   DACKAC010000110 DACKAC010000000
VERSION     DACKAC010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.114"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(91..1176)
                     /gene="xerS"
                     /locus_tag="I0B46_002625"
     CDS             complement(91..1176)
                     /gene="xerS"
                     /locus_tag="I0B46_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAR2084865.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
     gene            complement(3252..3770)
                     /locus_tag="I0B46_002626"
     CDS             complement(3252..3770)
                     /locus_tag="I0B46_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAR2084866.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         1580 a          786 c          730 g         1713 t
ORIGIN      
        1 tataaaaatg ataatgaagc ttgcctttaa agttgggctt ttatctaatt ttagagacag
       61 gcttcattat tatactattt tttactacat ttacaaagcg ttcaaagcat caattgtagc
      121 tgcattgtct atatgagtat ataaagttgt tgtatttgta cttgtatgtc ccaattgttg
      181 ggcagttaat accaatgaat ttgtctgttg atagagtcta gtagccaaag tatgacgtaa
      241 tttatgtgga ctgacttgga ctctgaatcc ttttgaatat tttgctacca tccgttcgat
      301 tgcaataccg gatatacgct tggcttctcc ttgataaatt gctagaaata aagctttttc
      361 gttttctgtt actttgtatc gttctttccg aatagctaga taattagcta aatattccat
      421 tgcaaatgct gctatattta ctgcgtctct cttatttcct tttctagtta ctaccacatt
      481 attcttttct aaagataggt cctctaaatt aatatttaca acctcggaga gtcttaatcc
      541 agatcctaaa atcaatgcat tgatcgccag gtctctttct acatctcgtt gataatagat
      601 caaagctcgt ttgctcaaag tttttttata ttcattggca atataatcta gataattttg
      661 tgtatcttcg tctagaaata attttttctc gattgcgttt gcacgggcag ctaacgtagt
      721 tttatctgtg atagttgcta cttgatccat aggattattt tctaagtatg gttttcctaa
      781 aggattattg gaacttttcg aaagatattt aaatagaacc tttaatgctg tgatatttct
      841 attgatagta gaagtagata gacctaagtt attattatct ttgagcttag tttttgttgt
      901 ctcttttttt acattttcct ttgtgcgacg tttcaaatga gatttgtaca agtttagatc
      961 ataggcagtt aactgtctta tcgaggtgat tgaagtatcg ggaacgatag actcactcag
     1021 aagccattca taaaagagct tataattttt tagatattct aaagtagttc gaggtgattg
     1081 atctttatcc tcacgatctt gaacaaaatc ttttatataa tcgggcatat gaagtatgat
     1141 tgtatctatt ttttgatata tttctgtatt tttcatgtta ttcatccttt cgataagtag
     1201 gggagaggag tatctctata attggactat accaattata gagtgttttg tcaattggta
     1261 tactctgaaa tttattaatt tatttcaaaa aagcagataa ataggtatag atttaataat
     1321 ttaaaataaa tggtatacat gaataggaaa ggaaacagaa ggaggcaatc ttaatttttt
     1381 aaattgtcta aattatatag aaagaacaat ctttctatat aattagacat aagagagact
     1441 acggttgatt tgatattaac aagcgtatat gaatgtatat acaaataaaa aaggggaggg
     1501 agaagttaaa gaaaattcag agaagtgggg cactcagtag tattaacaat ttaaaaggaa
     1561 tttcttaaag aaaatgggtt gcatcctgga gatacaattt ttttagataa agaaaaaatt
     1621 caagcaacaa ttcaaaaaaa tcttaactgc tgagattgat gtagcaatat ctcaatcatc
     1681 aacaaagtac gatcctgcct ttcaaaagct tattgataaa tgtttattca ttataaaaat
     1741 aaaagaaaaa tttatgtcag cttttttcat caaagtggta atgataattc gtctattaat
     1801 tgtatttttt aggaaatatg gtataattgt tttaacgaaa aggtaattca tgatacacca
     1861 aaaaccacta gctggcgggc tagtggtttt cttttacgtt ctattcagat taacgggtag
     1921 gcttactgtc ttttttggca ataattttgc aatcaagcca tttacagatg taatgcgcgg
     1981 atacttctgc taagattgca aaaaacatat cgaaaaggta attcataata caacctcctc
     2041 cccttgccat aataggccgg ggtagcagac agcataaata ttataccata actgaaaatt
     2101 tcattttagt acatttcaat atattgatga accagcacaa taaaacatgc catgactaac
     2161 agaaaaaaac ttattaaacc agatataact tgtaaacatc caactatata gaaaacgtaa
     2221 attttctata tagttggatt aagataacga ctatttattt tatcatattt ttactatacc
     2281 ctatactttt acttggtata tttatgaata atcgcaaatt tacccctata ctactagttt
     2341 gtaagttaga ttaataaaaa tactaaagtt atttgttctt ttattaaata cacctacaac
     2401 ttataaattg atggtattta gataacacga aagaataaaa ataacagctg atagtaaagt
     2461 ttgttgacga ttgctactca atcgaaagtg agttagtgcg tatgaacttg cttctgaaaa
     2521 cttatgaaat gaaagacctt ttacatcttg cagaaatcgg tactgggatt aaactaagca
     2581 ataaaaacta atgttcccct cattctttga acttctacta tttgatttat ttattctgat
     2641 aaatgacgat ttcaccttca ggatcttcaa atcccgtata gaatttattt aagatctctt
     2701 cagtatcagt aataggatag gcgataggcc acataccctt tgatggttag aataaactgc
     2761 tcaaaatctg tatcgtatgt tggctttagc atattaacaa aggctatctc caaattataa
     2821 accataagcg ttgaatcgtt cttaacaatt tcaattcttc ctaacccaaa gtcagaagat
     2881 ataattaccg gacttgcctt acccttcatg tgggttgttg agattccata tttaaaggga
     2941 attgtcggta ctatcggcgg tctgaaaaac atcccaaaaa taaaaaaatc tttcccctgc
     3001 tagtctccta agtccctttg tagcggatat ggcaccataa tctcatttta tcgaagtgag
     3061 tcatggttta tctgttggcc aaccaaaatt gatacaatct aacgatgcac cctctacggc
     3121 attctagggg agtgcacttg cattcaccca ataaaaaccg gcagttagca aagaacttat
     3181 gtagtcgtat gttcttactg ttaacataca agaggtctcc tttccacaat tgcttagcag
     3241 taaacgggaa tttacaaagc taatctcttc cattttctta tctttgttct aaaagtccca
     3301 aatggagcaa ctgtatttac atgaataaat ttatatactt cccatacagc cgttttggtt
     3361 gcctcgtctg cccattttct tttgtgtgca gtgaatagat cttcatcact cattgaatca
     3421 atcatcatgt taatatcatc aatattacga ttgagaattt cctctaattc tttcaatggt
     3481 agatgtgcat aggtatttgt aaaccactta tataactctc ctaactgatt ccatttaaat
     3541 tgatcagaag gcgttttaac agataatcca tttctttcat cattttccca ttttagaatt
     3601 aaattcgtcc aaccaacttg atatgctaaa ttttcagcag gtgttctatc cacttcctca
     3661 actcttttat ccttcaattc ttcaggaata gtttcaaatt ctaaaatata tttagtataa
     3721 gtcctattta tttctttttt taattcttct ttatttttat atgttctcat tatattacct
     3781 ccataaattc ctaattgtct cctttaattc atctataatt tcatccacga tattttaatc
     3841 cgttctacaa tgcatatctg atatgcattg actgtctaac tcatatgtga atgatttata
     3901 aaatatccca acgtctcatc taaagaaatg ttgatcgtca gcaaggagct aaaaaatacg
     3961 aatatatttc ttttgtagta aaatcggact ggtttgcata tcattttctt cgtaaatatc
     4021 aaaatcatgt taactactat caattactaa taaaaactac cactttaagc gttaagaact
     4081 gttaagttta gttaagtgag gatagttcac aaaatccatt gttaaacttc acgaaagtgg
     4141 taaaactcaa aatgctttat ataggattac agcatcgctc tttcttcgat tgcgcgttgg
     4201 gtaaaaaata ttatgaagta aaaattgacg atgacacgtt ccttacggct catcagatga
     4261 aacagctaca aaaacgaaat gatcaactcg aagaaaattt aattctaaaa aagcgattgc
     4321 catattcacg cctcgctcga cgaactatta aaggtcaaga tttacctgaa gatcatcctt
     4381 tttaggaaat tgaagctttt tatgttgcac aatgcattta taatactaca atgatgtttt
     4441 caccagatat tcttctaggg tgcggtgtaa tgaaacagat acacttgcaa aagaaaattc
     4501 accgcgaatt tactaaaatt atgaataatt atgttaatct tccgaaattt ttttccaaaa
     4561 ttaagggatc aagctggtgt tatagattgt ttaaggttgg caaaaaaata acttcttttt
     4621 aaacaattat agaaaaccga tactctgtta agttggttga cacggtgtcg atttttatga
     4681 ctttcttatt ctgaaacttc actaacggac agtacctaaa aagtctatag tatggacgtt
     4741 tacgagtact attttatcaa aaatgctgct catttcaact tatatgtttt agtgaactac
     4801 ccccactta
//
LOCUS       DACKAC010000111         4791 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.115, whole genome shotgun sequence.
ACCESSION   DACKAC010000111 DACKAC010000000
VERSION     DACKAC010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.115"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(75..1073)
                     /locus_tag="I0B46_002627"
     CDS             complement(75..1073)
                     /locus_tag="I0B46_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAR2084867.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKATIETDTTIETKKSKTQS
                     TEQTTTEMKQSVETSASSITESSSEEVKNTLWDTNKASKLETFVTQWGKTLGQEYKSY
                     NLQNNVSLYGTPLPQAVINGDWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVETGE
                     YLGQHVYFFGFQNGQPKVYLTQQNQGNENNYLYFNETQNQQLKQGFSDIVNGQTPQTP
                     VVEESTQQATTTTTQSTSSSGISSWEAAKESVLNNKALWLYDSPDKNVSNTNLISTNE
                     YMKDLMQDENGSYYSINIVTDDDGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK"
     gene            complement(1148..1444)
                     /locus_tag="I0B46_002628"
     CDS             complement(1148..1444)
                     /locus_tag="I0B46_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAR2084868.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHTGYRHYWERID"
     gene            complement(1437..1730)
                     /locus_tag="I0B46_002629"
     CDS             complement(1437..1730)
                     /locus_tag="I0B46_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084869.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            complement(1876..2427)
                     /locus_tag="I0B46_002630"
     CDS             complement(1876..2427)
                     /locus_tag="I0B46_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HAR2084870.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFLRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            complement(2601..2900)
                     /locus_tag="I0B46_002631"
     CDS             complement(2601..2900)
                     /locus_tag="I0B46_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HAR2084871.1"
                     /translation="MLETAIEVLEKCAQLVTASEEWGYESVTMEKEEIEMGMLPEDVH
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTDE"
     gene            complement(2927..3193)
                     /locus_tag="I0B46_002632"
     CDS             complement(2927..3193)
                     /locus_tag="I0B46_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAR2084872.1"
                     /translation="MDSLPVELQALCWNLIDQNVQKQFPLDYLQIFEFSTEKGNQKLV
                     HRQEEPEERKEYLISPKLRLKSVSQKIWAIDSGEYQTMLLPEEY"
     gene            3480..4223
                     /locus_tag="I0B46_002633"
     CDS             3480..4223
                     /locus_tag="I0B46_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084873.1"
                     /translation="MVVSKLPRNEDYLTNYDGYVVTTEHFDLANPVTKVDDIPDYEMY
                     SQTIDSLNKRFGNRVLKGIKIGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDD
                     EVKDMDPAIVIPQYFAQLSEALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPIL
                     DQVIKNKLAFELNAKSAYLYDNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFD
                     DLFALLKANGVTELAIYQKGKRIMVPLPV"
     gene            complement(4260..4757)
                     /locus_tag="I0B46_002634"
     CDS             complement(4260..4757)
                     /locus_tag="I0B46_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAR2084874.1"
                     /translation="MNNQITLAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
BASE COUNT         1428 a          912 c          696 g         1755 t
ORIGIN      
        1 taaaaaaact gattctaaga aaattaaata atttttctca aaatcagttt ttctttgata
       61 aataatattt tcaattattt aatcatttga aaattgttag tcattccaat acgttgagaa
      121 attcttccat ctgtataaac acgaaattgt tttccaccag ccatggtcgt gtttccatca
      181 tcatccgtta caatattaat tgaatagtag cttccatttt catcttgcat tagatctttc
      241 atatattcat ttgtgctaat taaattagta tttgatacat ttttatcagg agaatcatac
      301 aaccataaag ctttattatt tagcacagat tctttagcag cttcccaact tgaaattcct
      361 gaactacttg tagattgtgt cgttgtagtt gttgcttgtt gagtagattc ttctactact
      421 ggtgtttgag gagtttgtcc attcacaata tcactgaaac cttgttttaa ctgttgattt
      481 tgagtttcgt taaagtacag ataattattt tcattacctt gattttgttg cgttaaataa
      541 actttaggtt gtccattttg aaaaccgaaa aagtaaacat gctgccctaa atattctcca
      601 gtttctacat cagaataaac tgctactaaa ttgaaatctg catgaccagt tccatcttct
      661 gaccattgta cggtaactgg tgcttcattg attgccattt tccaatcgcc attaatcacg
      721 gcttgtggta aaggagtgcc atacagactt acattattct gaagattgta gcttttatat
      781 tcttgaccta atgtctttcc ccattgtgtg acaaaagttt ctaatttgga agctttattc
      841 gtatcccaca aagtattctt aacctcttca gaagaagatt cagtgattga acttgcactt
      901 gtttccacag attgcttcat ttctgttgta gtttgttcag ttgattgcgt tttacttttt
      961 ttagtctcaa ttgttgtatc agtttctata gtcgctttag tcctcgtatt ctcagatgta
     1021 caaccagtga ataaaattcc cattcctatt agtaataaca gccatttttt cattatattt
     1081 ccccctttca atttctccct cacgtactta ttctatcaga caattaattg atctaaatgg
     1141 taatctttta atcgattctt tcccaataat gtcgatagcc cgtatggcag cggtctcttc
     1201 cttgaggact ctcagatgga ttaaaactag tagaaatttc ccttccacat cctctacagc
     1261 gataagttct cattattttt gggctattag tattattttt cttagttaga gctatgagtc
     1321 caactatgat taccccaact gtcttaatta cttttccttt atctatattc ggatgcgcaa
     1381 gcatatattt atataaccaa tatccaccgt aaattaaacc acctaataca atcagcttag
     1441 ccatgtactc gtctcctttc tttttgttta attacttttt gaatccatct tgcagagaaa
     1501 ccataaactt ctgtcaattg acggatatct acagtttttt cctgttctat catgttttcg
     1561 attctttttc taactaactc acgatcgtac attttcattg gaaaagaaat ctgttgccct
     1621 cgaaaagcaa gataaaattg atagagcaat tctgaatcca gttccatttc ctcaaaaaag
     1681 tcattcatac ttttataaga gttactcaaa caagtttcat caatctccat ctacacaccc
     1741 cctgttattt tttacataat gaaaatagca tattactggg tacattgata attcatataa
     1801 catgtatcat cacatgtatt tttaaagtca aaaagcatgt aaaactactt ataagtaaca
     1861 tgagaaaata aaatgttatt tttcttgaaa taataattga ctatctgcca attgccattc
     1921 attacgaaca tgaatactta gtgggtggga taacttacct tctgcattta tcaatacttt
     1981 ggtcatcttc ttttgtttcg cactcaattg atcgtaaata gtctttgctt gttcataagc
     2041 cacttgactc gtcgctgtaa tcgaaccaca agcaaaaata atggtattga ttttcttttc
     2101 tgtaaaaaca gtcgttaata tttccaaaga ttgttcatca tttcctcgaa gaaatacccg
     2161 aggacttttc gcttttgtgt acgaaaaaag attcacagca ataaaaccag taaatcctaa
     2221 ttgtcggata ttcttttcga ttaataacat actcaaatca ttcgtaaatg gttctgtatc
     2281 tgttggacga atagtaatga ctactgctaa tttatcttcc gcttcttttc ctttgatcgc
     2341 ccattgtcgt ttgaaaacat agcgatgggt atgatgagga tctcgccaaa cttctgtttg
     2401 tgtggtcact aattcttttt caatcatctc tttttctcct atgctatttt ttgttcaagc
     2461 acttcaatta ccattgccgt tataagtgtc aatactagtt gaaaatattt catattcttt
     2521 ctcctttttt cttcagaatt tgatgaatcg cggtaaggat atcttctaaa catttcgcaa
     2581 tcattctcaa ataatcgatc ttattcatcc gttcctccaa tctgactcca aaccaatcgt
     2641 ccttctacca gtaacccacg aacaaattct cgctggctgg taatatctgt aatgaaataa
     2701 actacatagt cttttccatc ttctggtgcg caataaatat aaagatgtgt catcactaaa
     2761 cgtggtagat gaacatcttc aggaagcata cccatttcaa tttcctcttt ttccatcgta
     2821 acggattcat agccccactc ttcagaagca gtgaccagct gtgcacactt ttccagtact
     2881 tcgatagccg tttctaacaa gtcctttcgt tcctttcttt tagatactag tattcttctg
     2941 gtaataacat cgtttgatac tcacccgaat caatggccca tattttttga gacactgatt
     3001 tcaaacgaag cttaggtgaa atcagatact cttttctttc ttccggttct tcttgacgat
     3061 gaaccaactt ttgatttcct ttttccgtag aaaattcaaa aatttgaaga tagtctaaag
     3121 gaaattgctt ttgaacgttt tgatcgatta gattccaaca taaagcttgt aattccaccg
     3181 gcaaactgtc cattactccc tttgtgacat aacgttatcc tttattttga aacatttctt
     3241 ttccctcctt tgccataaaa atagcccaat cttctattaa agaagattgg gcttcatgga
     3301 tataatatat atttgtaaat aaagttaatt cagttataat tatcaaaact acacaattat
     3361 taaagttttt catatctctt tgtttagtaa gtttttctaa atcttaaccc gtggtgctag
     3421 ttaatttttt cctagaggaa aatgtctaaa catgttggta aatcaacaaa aaaacgcata
     3481 tagtagtctc aaaactccca agaaatgagg actatttaac aaactacgat ggttacgtcg
     3541 tgacaactga acattttgac ttagccaatc ctgttactaa agtcgatgat atccccgatt
     3601 atgaaatgta cagtcaaaca atcgatagct taaataagcg ttttggcaat cgtgttttaa
     3661 aaggcatcaa aatcggttac atcgcttctg aaaaggatcg gatcatcgac tatttagccg
     3721 ataaagatta cgatttaaaa ttactcagtg tgcatcataa tgggcaattt gattatttag
     3781 atgacgaagt caaagatatg gatcctgcta ttgtgattcc tcagtatttt gcacaacttt
     3841 cagaagcact agttgttata gaagcagatg tctttgctca ttttgactat ggttttcggg
     3901 tatttggtct atccgttgct gaatttaaac aatatgaagc gcagttttta ccgattctag
     3961 atcaagtaat caaaaataaa ttggcattcg aattaaatgc taagagcgct tacttgtatg
     4021 acaatttagc gttatatgaa tatgtcatcg atttatatct ttcacgtggt ggtaccttgt
     4081 ttagtgttgg atcagatggg cattaccttg aacatttccg tttccatttc gatgatcttt
     4141 ttgctctgtt aaaagcaaat ggtgtgacgg aattagcgat ttatcaaaaa ggaaagcgaa
     4201 ttatggttcc attaccagtc taaaaaaacg tcctcagcat caaaagtgag gacgtttttt
     4261 tagatttctt tttctaaaat cggacaaata cgatcttgtt ttcctaaagg aaaatgtttg
     4321 atcccgattt ttttaaatcc atgacgttca taaaaacgaa tacctgcatg atttttttca
     4381 taaacagtca aacgtaaata acgtttttgt ttcgttttag cataacgatt cgcttcatcg
     4441 agtaaccgtt gccctaaccc tttgttttga taatctttga gcagatagag ccgttgaatc
     4501 tctatagcat ctttttcatc attgactttc atataaccaa tgagtatttg atgatcctta
     4561 ataccaataa atttactggt tgactgtgcc aattctactg ccaatatttc agttgtcaat
     4621 tgtgtctgta aatatccttg catatcaagt ggatctgcgc catcgataaa cgattcataa
     4681 aaactgacaa cacttacaac ttgtaaagca tctagatcat cataatctaa atacgctagt
     4741 gtgatttgat tgttcatcta actcctcctt ttgacatagt atactataaa t
//
LOCUS       DACKAC010000112         4726 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.116, whole genome shotgun sequence.
ACCESSION   DACKAC010000112 DACKAC010000000
VERSION     DACKAC010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.116"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            130..1275
                     /gene="tgt"
                     /locus_tag="I0B46_002635"
     CDS             130..1275
                     /gene="tgt"
                     /locus_tag="I0B46_002635"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAR2084875.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1372..1752
                     /gene="yajC"
                     /locus_tag="I0B46_002636"
     CDS             1372..1752
                     /gene="yajC"
                     /locus_tag="I0B46_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAR2084876.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKKEK"
     gene            2057..4654
                     /gene="adhE"
                     /locus_tag="I0B46_002637"
                     /gene_synonym="adhC"
     CDS             2057..4654
                     /gene="adhE"
                     /locus_tag="I0B46_002637"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAR2084877.1"
                     /translation="MAKVMEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     AQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
BASE COUNT         1655 a          834 c         1010 g         1227 t
ORIGIN      
        1 ataaaaaaag gataaaaaaa agaatattaa ataaaataga aggaagccga gggaacttgc
       61 aaaaagcact tgaaacgatt atgatggtgg gagttgtcaa gatattttat atagaaagta
      121 gggaaatgaa tgacgcaacc tgccattcgc tatcgtttaa tcaagaaaga aaaacatact
      181 ggcgcgcgtt taggggaatt gatcacgcca cacggaactt tccccacacc aatgttcatg
      241 ccagtaggaa cattagctac agtaaaaaca atgtccccag aagagttgaa agaaatgggt
      301 gctggcgtta ttttaagtaa tacatatcat ttatggctac gccctggtga agacttagta
      361 gaagaagctg gcggactgca taaatttatg aactgggatc aaccgatttt aacagattca
      421 ggcggctttc aagttttttc attaagtgat atgcggaata tcgaagaaga aggcgtgcat
      481 ttcaaaaatc acttgaacgg ttcgcgtatg tttttatcac cagaaaaagc aatcaacatc
      541 caaaataaat tgggctctga tatcatgatg agttttgacg aatgtccacc attcgatgaa
      601 agttacgact acgtgaaaaa atcagtagaa cgtacgtctc gatgggcaga acgaggattg
      661 aaagctcatg ctaacccaga ccgtcaagga ttatttggaa tcatccaagg agcaggattt
      721 gaagatcttc gtcgtcaaag cgcgaaagat ctagtcggga tggatttccc tggttactca
      781 atcggcgggt tatctgtcgg ggaacctaaa tcagaaatga atcgtgtatt agaattcact
      841 acaccgttga tccccgaaaa caaaccacgt tacttgatgg gtgtcggtgc tgctgattca
      901 ttgatcgatg gtgttatccg tggagtagac atgtttgact gtgtcttgcc tactcgaatc
      961 gctcgaaatg gcacatgtat gacttctcaa ggtcgtctag tagtgaaaaa tgcgaagtat
     1021 gctcatgatt tccgtccaat tgatgagaaa tgtgattgtt atacatgtaa aaactataca
     1081 cgtgcctata tccgccattt gatcaaatgt gatgaaacat ttgggattcg tttgacttct
     1141 taccacaatt tatacttctt gttgaatcta atgaaacaag tacgtcaagc aatcatggat
     1201 gataatttgc ttgaatttag agaagccttt tttgaagaat acgggttcaa taaagaaaac
     1261 gcaaaaagct tctaaaggaa aatcttttaa aaattaagta aaaaactagt gttagcagca
     1321 tttttctgct aaagtgtttt ttgtagatac agaattagtg aggtgaaaaa aatgggaaca
     1381 ttaccaatgc ttgtgatgtt tatcgcgcta ttagcaatgt ggttttttat gtcacgttcg
     1441 caaaagaaac aacagcaaga acgtcaaaac ttgttagata gcatgaaacc aggtgattca
     1501 gtcgtaacga tcggcggatt acatggagtt gtttcagaaa tcgacaatga caaacggaca
     1561 gttgttttag actgcgaagg gatcttcctt gagtacgatc gtgcagcaat caaaacggtc
     1621 aaaccaggga ctgtcgtaac aaatgatgca acaactactg ttgaaacaac agaagaacca
     1681 gctgagacag ttgaagtaaa aactgaaaca acagaaacgc tggaagagac agaagagaag
     1741 aaagaaaaat aagtatcaaa gcttatttaa agacgaggta gggcagaaag atgtcctacc
     1801 tcatttttta ttcaaaaaag aaagagataa gagtatagga tatattgctc aatattgaat
     1861 aatcgggtgt gtcatataaa taataataaa taactttata tatttttcta tacaaacgcc
     1921 tataaataaa gcaactttat atttaaaaat aactgtgaaa aaaatcacaa aaaagtgttt
     1981 acatagaaaa ataaatattg tatgatatac atgtgaatga tgtaacaaat aaccatgacc
     2041 cattaggagg ataatgatgg ctaaagtaat ggaaaaagaa aaaacgaaga caattgacgt
     2101 acaagcaatg attgatgatt tggcagaaaa agcaaatgca gcattgaaag aaatggaaag
     2161 ctttgatcaa gaaaaagtgg accatatcgt gcatgaaatg gcaatggcag ctttagatca
     2221 acatatgcca ttagcgaaga tggctgtaga ggaaacaggc cgcggtgttt acgaagataa
     2281 agcaatcaaa aatatgtatg cgtccgaata tatttggcat aatatcaaac atgataaaac
     2341 agttggtgta atcaatgaag atgcccaaaa aggattgatt gaaattgctg aaccagtcgg
     2401 tgtggtttgc ggagtaactc caacaacgaa tccgacttcc acaacgattt tcaaatcaat
     2461 gatcgcgttg aaaacaagaa atccaatcgt ttttgctttt catccaagtg cccaaaaatc
     2521 ttctgcagaa gcagcacgag tagtacgtga tgctgctatc gctgccggag ctcctgaaaa
     2581 ttgtatccaa tggatcgaac atccttcgat cgaagcgact tctatgctaa tgaaccatcc
     2641 aggtatcgcg atcgtcttag cgacaggtgg agctggaatg gtcaagtctg cttactctac
     2701 aggaaaacca gctttaggtg taggtccagg taacgtacca gcttatattg aaaaaacagc
     2761 aaaaattaaa cgtgcagtga acgatttgat cgtttcgaaa acatttgata atggaatgat
     2821 ttgtgcttct gaacaagctg tgatcgtgga taaagaaatc tatgcagcag taaaagcaga
     2881 attccaagca caccaagttt atatcgtaaa accagatgaa ttgcaaaaat tagaagatgc
     2941 agtgatgaac gaaggaaaat atgcggtcaa tccatcaatc gttggacatt ctgcgatgga
     3001 aatcgcaaaa ttggcaggaa tcagtgtacc aaaaggaacg aaaatgctga ttgctgaatt
     3061 ggaaggcgta ggcccagatt atccgctatc aagagaaaaa ctttctccag ttcttgccat
     3121 gatcaaagct aacaacacgg accatgcgtt tgatttgtgt gaaggtatgt tgaatcttgg
     3181 aggattagga catacagcag ttatccattc agaaaacgaa gagttgcatg tcaaattcgg
     3241 tcttcgaatg aaagcttgtc gtattttagt gaatacacca tctgcagaag gcggcatcgg
     3301 tgatatctat aacgaaatga ttccgtcatt gactttggga tgcggttctt atggtaagaa
     3361 ctctgtttct cgtaacgtct ctgctgtaaa cttaatcaat gtcaaaactg tagcgaaacg
     3421 gagaaataac atgcaatggt tcaaattacc gccaaaaatc ttctttgaaa agaactctct
     3481 attgtactta gagaaaatgg aaaacgttga acgtgtcatg atcgtttgtg accctggtat
     3541 ggtccaattc ggttactgcg atacagttcg tgaagtattg gcacgacgta aaaatgatgt
     3601 gaagatcgaa gtcttctcag atgttgaacc gaacccatcg acaaataccg tatacgcagg
     3661 aacgaaactg atggcagact tcaagccaga tacagtaatc gctcttggag gtggttctgc
     3721 aatggacgca gctaagggaa tgtggatgtt ctatgaacac ccagacacat ctttctttgg
     3781 tgcaaaacaa aaattcttgg atatccgtaa acggacatat aagatcgaca aaccagaaaa
     3841 aacacaattc gtatgtatcc cgacaacatc agggactggt tctgaagtaa cgccatttgc
     3901 ggtcatcact gacagtgaaa cacatgtgaa atatccgtta gcagattatg cattgacgcc
     3961 agatgtcgca atcgtcgatc ctcaattcgt catgtcagtt ccagcatctg tcacagcaga
     4021 taccgggatg gatgtgttaa cacatgcaat cgaatcctat gtttcagtca tggcttcaga
     4081 ttatacacgt ggattgagcc tacaagcaat caagttagtc tttgactatt tagaaaaatc
     4141 agtaaaaaca cctgatatgg aatcacgtga aaaaatgcac aacgcttcta caatggctgg
     4201 tatggcgttt gctaacgcat tcctaggtat ttgtcactca gtcgcacata aaatcggcgg
     4261 ggaatacggg atccctcatg gacggacaaa tgcgattctt ttaccgcata ttatccgcta
     4321 taatgcaaaa gatccacaaa aacatgcgat gttccctaaa tatgactatt tccgtgcaga
     4381 tacagattac gcagatattg caaaattctt aggtctgaaa ggcgaaacaa cagaggaatt
     4441 agtcgaagct ttagcaacgg ctgtttatga attaggaaaa tctgtcggca tcaacatgag
     4501 tctaaaagct caaggagtca cacaagaaac actagataca accgtagacc gtatggctga
     4561 attagcttac gaagaccaat gtacgacagc taatccgaaa gaaccattga tctctgaatt
     4621 gaaacagatc atcattgatg cttacaacgg ataagcaaaa gaaagcgtag aaaaaagata
     4681 agaaaataga tgaaaaagaa cttggaaaac gttgtttctc caagtt
//
LOCUS       DACKAC010000113         4612 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.117, whole genome shotgun sequence.
ACCESSION   DACKAC010000113 DACKAC010000000
VERSION     DACKAC010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.117"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(379..1332)
                     /locus_tag="I0B46_002638"
     CDS             complement(379..1332)
                     /locus_tag="I0B46_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAR2084878.1"
                     /translation="MKKLVVGILILASFGLAACGNSDNTSQADTKKSSTQAETALTIK
                     DSNGDQIEVPKNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(1355..2110)
                     /locus_tag="I0B46_002639"
     CDS             complement(1355..2110)
                     /locus_tag="I0B46_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAR2084879.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKKLVQEIGKTIVIVLHDINFAASYADEIIAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(2107..3066)
                     /locus_tag="I0B46_002640"
     CDS             complement(2107..3066)
                     /locus_tag="I0B46_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAR2084880.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFAWALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(3059..4009)
                     /locus_tag="I0B46_002641"
     CDS             complement(3059..4009)
                     /locus_tag="I0B46_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAR2084881.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFPQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            4159..4611
                     /locus_tag="I0B46_002642"
     CDS             4159..4611
                     /locus_tag="I0B46_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAR2084882.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPVEKEKPKKKKSFWDRLF"
BASE COUNT         1503 a          969 c          786 g         1354 t
ORIGIN      
        1 tggacttatt gccgagaagc agcagatgga acatcgttta aagaagagat cggtacaaaa
       61 taaaaatatc taaatttaag ttttatctaa gtttcgcttt gtacctttga ttcaaatcaa
      121 aggaggcgat cttgatgctg aaaaaatgga tcacactatt ttttgccact ccccattcta
      181 gaatcaaccc gtatgatgta gagaatgaat tcacgttgaa aaaagatttc ccaaataaat
      241 aaaaaccatc cgtaaaatca tcaatacgat tttacggatg gtttttgata acttctttga
      301 ggataggctc ttgctattta agaatgtaag aaacattatt gttacatagt atgagagttt
      361 aatcctctgt tatttatgct atttaaatgc ttgattcaca tcttcgatca tcagtttcat
      421 tgattctaaa ccgcccccgc ttaaatacca gacatctggt tcaagggaga tgatctgctg
      481 atttttacca gcattcgttt gtgctaccag ttcattggca gaaatatcat ctttgctgtc
      541 gtctccacca atagcttttg ttctgtctac tacaaataag atatccggat ttttttccag
      601 cacatattca taagaaacac tttgaccgtg agtggatgct tcgatttgat catctgcttg
      661 ttcaaagcca aataaatcat ggatgaaacc gaatctagaa ccagatccgt aggctgataa
      721 ttgcccttca ttcacaagtg tgactagtgc ttttttgtca gtagcttctg ctttcttttt
      781 cgtctgatcg attgcactag ataattcctc taattgagtt ttagcttctt cttgcttacc
      841 aaagatctcc cctaaagcag tgacattttg ctggaaagat tcccatggat ttttcgcatc
      901 taaagaaagg aaaatggtcg gagcaatttg acttagatct ttttgaaaat ctctttgccg
      961 gccagaaatg atgatcaggt ctggctgaag ctgattgatt ttttctaagt ctggttcttt
     1021 gatcccacct gctgattcca cttttttata ggatgaaaga tactcaggaa ggctgcttgt
     1081 agctgctcca actaccttat ctccaacacc aagagcatcc atcgtatcta aagaaccatt
     1141 gtcgaatacg acgacttttt caggattctt tggcacctcg atttgatcgc cattactgtc
     1201 ttttatcgtt aatgccgttt cagcttgcgt actgcttttc tttgtatctg cctgactggt
     1261 gttgtcacta tttccacatg ctgctaaacc aaatgatgct agaatcaaga tacctactac
     1321 taattttttc atctcttctc tcctaatgtt agttttagaa ataaagacag aatctgcgac
     1381 cttcaatctc acaaattcgt atgtccattt cgtacaattc gtccagtaca tcttttttta
     1441 tcatttcttg ggtcgttcct tgcgtgaata actgcccatc tttcattgca ataatttcgt
     1501 ctgcataact tgcagcaaag ttgatatcat gaagaacaat aacaattgtt ttcccgattt
     1561 cttgtactaa tttttttaag atctgcatga tttgaaccgc aaaattcata tctagattgt
     1621 tcaaaggttc atccagtaaa atataatccg tgtcttgcgc tagcaccatt gcaatatacg
     1681 cacgttggag ttgaccgcct gaaagagtgt caatcgattg atccgctatt tcttctaacc
     1741 caaggtgttc cagtgattcc tgaacgattt cctcgtcttt tgtcgtcaat tttcctttcg
     1801 tataaggaaa tcgtccaaat tctactaatt ctttcacggt gatttggaca tttatttcat
     1861 tcgattgtcg taggatcgat aattttttgg ctaattcatt ggatttccaa gcctttactt
     1921 cgttatggtc taaatagatt tcacctgtat cttttggtat caaacgactg atcatcgaca
     1981 acaccgttga ctttccagca ccatttggac cgataaaagc agtcaacttc ttttcaggaa
     2041 tcgttaactg tacatcttta accactgatt ttccatggta cagtttattg accttttgta
     2101 aatcaatcat aggatactct tcctttcgcg aatcaatttt gtgacaaaat aaattcctcc
     2161 gacaaattca acgacgacac tcactgttgt attccagccg aaaatctgct ctactgcgag
     2221 ctgtcctcct gtcagaaaca gcatagctac cagaaagctt ccgataaata gctgaccatg
     2281 acgataagta gaaaagacac gataactgat atttgcgaca ataaacccta aaaaagtaac
     2341 tggacccaca agagccgttg atgtccctgt caaaagactg acacaacata aaatcaaaaa
     2401 ttcaaaacga tctgtgtcta tcccgaggtt tactgcttgt tcatttccga gatggaaaac
     2461 gtctagcaca tgtcgatatc gaaataacag gtaccagcag ccgataatga tcaaagaagc
     2521 aggcaaaaga tactgactct ggatattgcc aaagcttgca aataatctgc cttgaagcaa
     2581 atcatattca ttcggatcca tgatgacttg taaaaaggta ctgacactac caaaaagcgt
     2641 acctaaaatc atccctgaca tcagtaataa aaaaagattc gctctcattt ttttcaaaag
     2701 aaagaaaaat aacaatagac tcagcccgac catcagccca acgttcaata agaaaaaaac
     2761 tgtacctgtc tgctgaagca tcgtcactcc tccgataaag aagaaaagga ccgtttggac
     2821 aaggacatat aacgactcaa gtcccaatac tgaaggagtc aaaaactggt tatgcgtaat
     2881 tgtctgaaag ctgattgtgg cactgctgac agctagcgag acaaggataa atgcaaaaat
     2941 tttcttactc ctcaatgccc atgcaaaatg ccagtttcca tatgtatgga tcgtcaagta
     3001 cattcctgta acaaataaaa caagaacaag cataccaata aaaatagatg gctttttttt
     3061 aagcatatcg tcgccccctt atcagaagat aaaggaagat agccccaccg atgattccaa
     3121 cgatcacact gactggtatt tcataaggaa aaatcaacac gcgagcgaca agatcgcaaa
     3181 atactaagaa caaactacct gctaaagcag ttacgccaat tgttttgtgc acttgatctc
     3241 catatattct agagacaaga ttcggaatga tcactccgat aaaaggaatg cttcctacca
     3301 tgatcaacat gactgcacta gccaatgata ctaagccaat gcctaaaaac tgcatcttct
     3361 ggtaatctat tcccaaattt actgcaaacg attcgcccag accaatgatc gtaaatcggt
     3421 aagcaaatag ccaaaccaag agcaacaatg gaacaacagc atacaataac tcataatttc
     3481 ctcgtatcac agtcgcaaaa tttccttgaa gccatgaagt catattttgt accaactgga
     3541 attgataagc aaaaaaagta gcaactgaac caatgatatt ccctaacatg actccaatca
     3601 acggaagcat caattgattt ttctggggga acaatcgcgt aagggataaa aaaagcaatg
     3661 ttcctgcaaa agcaaagcaa aatgctgtca aggatcggac aatcaaggat gcaccaggta
     3721 ggaaaatcat agccactaaa atacctaaac gcgcactatc catcgtgccc gcagtcgttg
     3781 gtgaaacaaa tttattttgg gtcaaatgtt gcatgatcaa cccacaaata ctactggtcg
     3841 ctcccacaag gatcaaacaa atcgttcgtg gcaaacgagt cactagcaag atttgtaagc
     3901 tttgcccatc taactggagc agttctgaca attgtacatt cgatactccg atgaacaatg
     3961 aaatactact taaaataact aataatgcta ctagtcgtcc gtagcgcatc caatccacct
     4021 ttctaattga gaatgatttt cattcttatt ttaagtgaat cgttgaacaa aagcaaccgc
     4081 tttttcatat gttttaggta ttattgggaa tatgctataa tcgtaagaaa gacaaagaga
     4141 agaaggaggg tattttttat ggcacaagat gatgtaaaaa gccatttgga taagggactt
     4201 tatggtacac ctttagtgaa ccctgaagaa cagcataaat atttagggac attcagggaa
     4261 cgctgctatc ttagtatgac tgttgctgaa atgatccaac caaaaaacaa agaaaacttt
     4321 ttaaaagaac tcaaaaaaca tccagatgct tccatactct tgaatggggc tctacccctt
     4381 tctgtccaaa accaatatat ccagctagca acgaaaaaca atagccgatt tactgtagtc
     4441 aacgattacg tcactgcaaa tcctgaggaa ttcggattgc tcttaacagc aaaagaagca
     4501 gtaaatgaac ctgtgattga tatcgaaaaa aaatacccag aagaaaactc ttcgccagtt
     4561 gaaaaagaaa aaccaaaaaa gaaaaaaagc ttctgggatc gtttatttta aa
//
LOCUS       DACKAC010000114         4539 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.118, whole genome shotgun sequence.
ACCESSION   DACKAC010000114 DACKAC010000000
VERSION     DACKAC010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.118"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(508..849)
                     /locus_tag="I0B46_002643"
     CDS             complement(508..849)
                     /locus_tag="I0B46_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAR2084883.1"
                     /translation="MKPNEIVTVYVAFTDKNGGKRRPILVVSDKEDRVEFFGITSQYE
                     KKSDQIKRVYFPIAEWEKAGLKKQSWIDVGSLKAIPKSRENISFKKVGMLSASDTKKL
                     NRFIEKLQRKV"
     gene            complement(839..1120)
                     /locus_tag="I0B46_002644"
     CDS             complement(839..1120)
                     /locus_tag="I0B46_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HAR2084884.1"
                     /translation="MKVKNPTEKSRIQVGIDKNLKENAEMILEELGLNPTTAITILYK
                     QVVARGEFPVEIKLSEEEKQGIRLQQLTKDMPVDVLDTDEKLEEWFDEA"
     gene            complement(1491..1730)
                     /locus_tag="I0B46_002645"
     CDS             complement(1491..1730)
                     /locus_tag="I0B46_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084885.1"
                     /translation="MTKFKSKGMQWCSYYYVGNDEDLLRKTQRKGYPDKILKQIGYYM
                     PEERIEKEFCEVLFDSEQLLVLLTQNKLICQFENN"
     gene            complement(1750..1998)
                     /locus_tag="I0B46_002646"
     CDS             complement(1750..1998)
                     /locus_tag="I0B46_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084886.1"
                     /translation="MEGMDFLDHEDLVDFGYTWKGMVGISRSLANAFYERNYAVYVLY
                     DDDTESLVDEEYKLDLENVLYGIEKEDLAKYIFSWLGQ"
     gene            complement(2013..2579)
                     /locus_tag="I0B46_002647"
     CDS             complement(2013..2579)
                     /locus_tag="I0B46_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084887.1"
                     /translation="MFEKRKDQFPNLEAKSQKVIDGEMIELFQKSYGTIETTASCKYY
                     FQGKRNTFKGFADSDEELAKKIIDWLVSSEHVAREDTVRTTINGEVLTGTRCVCCNHE
                     FVEDEGCYRINEYGDFICQSCHEIEFPTERSWFEYMLQDYEMEKENGEVVFCTKEELS
                     KRSDDEIEDWCVWLVGADDCVAYYTTAE"
     gene            complement(2580..3125)
                     /locus_tag="I0B46_002648"
     CDS             complement(2580..3125)
                     /locus_tag="I0B46_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084888.1"
                     /translation="MGERTQALITVKYGNSKTKASVSIHYQWGYGRYMLMDVLNQGIY
                     LINKAYDLRDKSREELAKGIFDRSTGVINFVTYESGVSAYDETGFGGRYEPYQETIED
                     VMKFSDNNDGYAHLEMVFDENGFSLDDKTRLRLFDFEGQQIGLNKFCESGKEFATKDF
                     IKGYRLLLENYGINLEEESVK"
     gene            complement(3151..3918)
                     /locus_tag="I0B46_002649"
     CDS             complement(3151..3918)
                     /locus_tag="I0B46_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAR2084889.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRI
                     GRSKPRAFILIKEKYLNEWNSTYEMVMTDNFEKVLDFRNALLGSDMGNSSLESIEVEN
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYEFPSENDKSF
                     TTNLKNAGKTIGIELLDHIIVTSKNTSYYSFQENGSL"
BASE COUNT         1288 a          953 c          593 g         1705 t
ORIGIN      
        1 tcgctacgcg accgttcgtt cgtcttttcc tccgtgggac tcacccaccg caagcggttg
       61 gttgagttgt ggctcctatt caaaattcac taatctgtta ttttttattt ctattcaaat
      121 aggtaacgtt cgtttgcaag caaactctct ctatgtaact tgccagttac atcagcggta
      181 acgctacgtc gcaagcgact tgctctaatg aaactgccgt ttcattctgc ctgttcgttc
      241 gtagcaagct acttcactca ccgtatccgt ctgaattatt gttatttatt gctattcaat
      301 atccgtcaga cattttattt atttacatac attttatata aaatatatat tataattcgt
      361 acattactaa atatcattat ttaacaattc ttaacgtttt attaatgttt ataaccacgt
      421 atatatttac gtatattttc atatacataa atacgtttat ataaacgtat atcttaatat
      481 acatataaac aaaaaagaaa agccttgtta gacttttctt tgtaatttct cgataaacct
      541 atttagcttt ttcgtatcac tagctgataa cataccaacc tttttgaatg atatgttctc
      601 tcggcttttg ggaatagcct ttaaagaccc tacgtcaatc catgattgct ttttaagtcc
      661 tgctttttcc cattctgcaa taggaaaata caccctcttt atttggtctg attttttctc
      721 atattgactt gtgattccaa agaattctac tctatcttct ttatcactaa ccaccaaaat
      781 aggtcttctt ttaccaccat ttttatctgt aaatgcaaca tataccgtta ctatctcatt
      841 aggcttcatc gaaccactct tctaactttt catctgtatc tagaacatct actggcatgt
      901 ccttagttag ctgttgcaat ctgattcctt gcttctcctc ttcacttagc tttatctcta
      961 ctggaaactc acctctagcg actacttgtt tgtaaagaat tgttattgct gttgtaggat
     1021 ttaaacctag ctcttccaat atcatttctg cgttctcttt taaattcttg tctattccga
     1081 cttggattct gcttttttca gttggatttt tgaccttcat ttcatccact ccttttgagt
     1141 ataaaacact cattatttat caaattgtcc acatttatga ttatattata ctcttaataa
     1201 actacattgc aagtaatcta atcaaattaa gatgctatat catacggtaa ttgaacaaat
     1261 tgtcgctaac ccacacatac ggaatcgtaa tgaatgaaaa aaagacaagc ctattcgcag
     1321 taggcttgtc tttgtgcttc tcttgtacaa aatcttcaca tttgtaaatt aattttagca
     1381 cctttgttag gaaagaacaa cttaaatgag atgctaaaga attaatagtt ctcaatctag
     1441 catattcact taataccatc ttatcttcat tcatggttca ccacctcatc ttaattattt
     1501 tcaaattggc aaatcaattt attttgggtt aacaaaacta atagctgttc actatcaaaa
     1561 agaacctcac aaaattcttt ttctatcctc tcttctggca tgtaataacc tatttgtttc
     1621 aatatcttat ctggataacc ttttcgttgg gtttttctca ataaatcttc atcatttcca
     1681 acataataat aagaacacca ttgcataccc ttacttttaa attttgtcat gtttctatct
     1741 tcctttcact tattgaccca accatgaaaa aatatatttt gctaaatctt ccttttctat
     1801 tccatataaa acattctcta aatccaattt atattcctcg tcaacaaggc tttctgtatc
     1861 gtcatcataa agaacatata ctgcataatt tctttcgtaa aaggcattag caagacttct
     1921 gctaatacct accattcctt tccatgtata accaaaatca accaaatctt catgatctag
     1981 aaaatccatt ccttccatgc tgattcacac tcctattctg ctgttgtgta atatgcaacg
     2041 caatcatctg cccctactaa ccacacacac caatcttcaa tttcatcatc gcttctctta
     2101 ctcaattcct ctttggtaca aaaaactact tcaccatttt ctttttccat ctcataatct
     2161 tgtagcatgt attcaaacca acttctttcg gttggaaatt ctatctcgtg gcaactctgg
     2221 caaataaaat caccatattc attgattcgg tagcaacctt catcttctac aaattcatga
     2281 ttacaacaaa cacatctagt acctgttagg acttccccat taatcgttgt tctaacggta
     2341 tcttcccttg ctacgtgttc actgctgaca agccaatcaa tgattttctt tgccaattct
     2401 tcatcagaat cggcaaagcc tttaaacgtg tttctctttc cttgaaagta gtatttgcaa
     2461 cttgcggtag tttctatggt tccataactt ttttggaata attcaatcat ttcaccatca
     2521 atcacttttt gacttttcgc ttctaaatta gggaactggt cttttctttt ttcaaacact
     2581 tatttcacgc tctcttcttc taggtttatt ccataatttt ctagcaataa tctgtatccc
     2641 ttaataaaat ctttcgtagc aaactctttt ccgctttcac aaaacttgtt tagtcctatc
     2701 tgttgaccct caaaatcaaa caatcgcaat ctggttttat catctagact aaatccattt
     2761 tcatcgaaca ccatctctaa atgagcatag ccatcattgt tatctgaaaa cttcatcaca
     2821 tcttcaatcg tttcttgata aggttcatag cgcccaccaa accctgtttc atcataagca
     2881 ctcactccag attcataggt aacaaaattg ataactcctg tacttctatc aaagattcct
     2941 tttgctaatt cttctcttga tttatctcgt aagtcgtatg ctttattgat taaataaatt
     3001 ccttgattca atacgtccat cagcatatat cgaccatacc cccactgata atgaatcgac
     3061 acgcttgctt tcgttttgct gttaccatat ttgactgtaa ttaacgcttg tgttctttcg
     3121 cccatcttgt tttcctcttt tctctaattt ttataaagaa ccattttctt gaaatgagta
     3181 atagcttgtg tttttacttg ttacgatgat atggtctaac aattcaatac caattgtttt
     3241 tcctgcgttt ttcaaattcg tagtaaaact tttgtcattt tcacttggaa attcatatcc
     3301 gctcggatga ttatgtgata ctgcgatttt tgctgaattg tttagcaaag ctagttgaaa
     3361 aatctctctt ggtatcgcta ctgattgatt aattccacct ttgaaaactt cgctataagc
     3421 aatgacctca ttctttgtat tcagacacat cactagcaaa gattcactag cattgtttcc
     3481 aattaagtta gtagcaaagt ttgctagacc aaccgagctt gtgactctat aaaaacttct
     3541 atcttcactg atatattttt gttcgatttt caagacattc ttaatcaatc cttcaccttc
     3601 attctcggtc ttgttttcaa cttcaataga ttcaagactg ctatttccca tatcagaacc
     3661 caacaaagca tttctgaaat ccaatacctt ttcaaagtta tcagtcataa ccatttcata
     3721 ggtgctattc cattcattca aatatttctc tttgatgaga atgaacgctc tcggtttact
     3781 tctgccaatt ctgatatttt gtgtatcgtg aaaaatatag ttatcaagta aagtaccatc
     3841 gtacttttca tagttcccac tgatatttgt taacttgttt accatttctt gattaacttc
     3901 taaaattcta tctctcatgt tgaacgcttc tttctgtttt tgatttttgg tttttcaaaa
     3961 ttcttttttg tttggattgt tttagaggga ggtggttttt tcttcatcgc tatttggttt
     4021 tgcattttat gtttcttcct ttcgttcatt tatttatctt tttcgcctgt cttgccttgt
     4081 cgggagtgat tagaatgcgt tcttatctgc cggattaata caagtgttta agtaaattat
     4141 tttcgtaacg cttgcgttta gaaaaaaatt tacgccactt gtattaatcc aaagatgaga
     4201 taagctctaa acacttctga caaggtatga caggcgaaaa agataatggg cgtaaggaat
     4261 tataaatggc aaaaccaaat aaagagcgat gaatttcaat gctttcaggc gtagcttgaa
     4321 agcctaaaag tcttgaagaa tgaaagactt tttaccttca aagcagacct agtaatctta
     4381 aataggcgat ggctatttaa gattacggtg ttgacccact aaataaacag aaatggaacc
     4441 gatcaagcaa aatcggacta ggcgatggct aggacggttt gcggtggctt taggaaacgt
     4501 tcatggtgag atgagcatat ctagcaatgt cgggctaag
//
LOCUS       DACKAC010000115         4430 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.119, whole genome shotgun sequence.
ACCESSION   DACKAC010000115 DACKAC010000000
VERSION     DACKAC010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.119"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(543..2048)
                     /locus_tag="I0B46_002650"
     CDS             complement(543..2048)
                     /locus_tag="I0B46_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAR2084890.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="I0B46_002651"
     CDS             complement(2045..2731)
                     /locus_tag="I0B46_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAR2084891.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="I0B46_002652"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="I0B46_002652"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAR2084892.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1238 a          919 c          729 g         1544 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat
//
LOCUS       DACKAC010000116         4218 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.120, whole genome shotgun sequence.
ACCESSION   DACKAC010000116 DACKAC010000000
VERSION     DACKAC010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.120"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            10..210
                     /locus_tag="I0B46_002653"
     CDS             10..210
                     /locus_tag="I0B46_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAR2084893.1"
                     /translation="MALGRMSELGYSMMFGEVEKNFFMKHIKGRGYVDTGGSVISEFY
                     TPLVPKGYDFLDSISKLEKMND"
     gene            288..962
                     /locus_tag="I0B46_002654"
     CDS             288..962
                     /locus_tag="I0B46_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAR2084894.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            1195..1629
                     /gene="dcm"
                     /locus_tag="I0B46_002655"
     CDS             1195..1629
                     /gene="dcm"
                     /locus_tag="I0B46_002655"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAR2084895.1"
                     /translation="MRFLDLFAGIGGFRLGMEQASHHCIGFCEIDKFARRSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQRFSDTRGTLFFEIARF
                     TTLLRPKLLFLENVRGLLNHEGGATFETILRTLDGLGYDVEW"
     gene            1630..2337
                     /locus_tag="I0B46_002656"
     CDS             1630..2337
                     /locus_tag="I0B46_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAR2084896.1"
                     /translation="MLNSKAYIPQSRERIFLIGHSRDACTEQVFPIIGPSSTSDQNIR
                     NLVNINPSNRGMGGQVYGSDGVAPTLTGDEGIKIALPARDGISVAGLLPGKFEQGNRV
                     YEVTGTAPTLSTKQGGTKIMIRKNETEYQEVKPEDSVNLAFPNSTSRRGRLGKQSVHT
                     LLTGDQQAIVTDQYQIRKLTPRECWRLQGFPDWAFDRDSQVNSDSQLYKQAGNSVTVP
                     VIFDIARRLKELDVHGL"
     gene            2327..2617
                     /locus_tag="I0B46_002657"
     CDS             2327..2617
                     /locus_tag="I0B46_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084897.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            2876..4060
                     /locus_tag="I0B46_002658"
     CDS             2876..4060
                     /locus_tag="I0B46_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAR2084898.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            4075..4194
                     /locus_tag="I0B46_002659"
     CDS             4075..4194
                     /locus_tag="I0B46_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAR2084899.1"
                     /translation="MGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKGK"
BASE COUNT         1364 a          692 c          979 g         1183 t
ORIGIN      
        1 aactttcgcg tggctttggg tcgtatgagt gaactcggat attccatgat gtttggggag
       61 gtcgaaaaaa acttctttat gaagcacatc aaaggtcgag gttatgtgga tacaggtggc
      121 agtgtgatta gtgagtttta tacgccactt gtaccaaaag ggtatgattt cttagattca
      181 attagtaaat tagaaaagat gaatgattga gtgatcttca atactgactt tatacttcaa
      241 gtaacgtata atacttttat atagtaggaa gaaaggggat aattaatatg gaaagtaatg
      301 attcttattt agagtatgca aaagcaaata aggataagtt tatttcttat attatcaaag
      361 ggaaatcccc cgaagttgga cgagacgcag tttttatggc agggagtcct ggggctggaa
      421 aatcagaggt tgccattgga ttagctgaga attatgataa ccatgtgatt attgatgcgg
      481 attactttcg cacccagttt cctgattata atggaaaaaa ctctagtctc tttcaaaaag
      541 catcttcttg gttggtagag caggcattta gatatgtctt agatcatggc tattctttta
      601 ttctggatgc aacttttgca atattaagtg ctgaaaaaaa tatctcgcgg gcgttgaaaa
      661 aacattttcg agtgacgatt ttctatgttt atcaagaccc taaaatcgct tggaaattta
      721 cacaagaacg tgaaattgca gagggacgtc atgtgccgaa aaaggcattt attaacgctt
      781 ttttcaaatc aagagagaac attgcaaaag taaagagtaa acatccagat gtattgcttc
      841 atatcataat aaaagattat acgaataata tttccgaagt tcattatgct acggataatc
      901 tagaattatt tttaccacta gggtatagta ggaaagattt ggaggttcaa ttacatgact
      961 gagaaagaac aagtaacgaa aattgtaaag aaatacaata agagcattgc ggatttatcg
     1021 gaaaatgcta ctgcaaaaga gttcaaaacg gtgattaaat atgtagccga tcaggccaat
     1081 gaaaaacaac ggaaattagt tggattaaat aaaaaatagt aggacaaatt aataatgtat
     1141 gagccaagag caaaatgttc ttggcttttt tctttgcaat gaatgaggta atgtatgaga
     1201 tttttagatt tattcgccgg tattggtggc tttcgtttag gtatggaaca ggcgagccat
     1261 cattgtatcg gtttttgtga aattgataaa tttgctagac gtagttataa agcaattcat
     1321 gatacaagta aggaggtgga aatgcatgac atcacaagtg tatcagatga gtttattcaa
     1381 tccctcggac cagtggacat cctttgcggc ggatttccgt gccaagcttt ttcaattgcg
     1441 ggaaagcggc aaagattttc cgatactcga ggtactttat tctttgaaat cgctcggttc
     1501 accactcttc tccgacctaa gcttttattc cttgagaacg tccgaggatt gctcaatcac
     1561 gaaggagggg ctacgttcga gacgatcctc cgaacgctgg atggattggg gtatgacgtg
     1621 gagtggtagg tgcttaactc aaaggcctac attccccaaa gccgggagcg gatattcctt
     1681 atcggacatt ctcgagacgc atgtaccgaa caagtatttc ctatcattgg accgtcttca
     1741 acatctgatc aaaacatcag aaatttggta aatatcaatc cttccaatcg aggaatgggt
     1801 ggacaagtct atggttcaga tggcgtagcg ccaaccttaa caggtgatga aggaatcaaa
     1861 atcgcactgc cagcaaggga tggaatctct gtagcaggac tattaccggg aaagtttgaa
     1921 caaggtaatc gagtttatga agttactgga accgccccca ccttgtcaac gaaacaaggt
     1981 ggtacaaaaa tcatgattcg aaaaaatgaa acagaatatc aagaggtgaa gccagaagac
     2041 agtgtgaatc tggcttttcc taattccaca agtcgccgag gtcgtctggg aaaacagagt
     2101 gttcatacct tactaacagg agaccaacaa gcgatcgtca cggatcagta tcaaattcgg
     2161 aagctaacac ctagagagtg ttggcgactt cagggctttc ccgattgggc ttttgatcga
     2221 gattctcaag tcaattcaga tagtcagttg tataagcaag ccggaaactc tgtaacagtt
     2281 ccggtcattt ttgatattgc cagaagatta aaagagttgg atgtacatgg tttatgatga
     2341 gttgattgga gaaatttatt gggtgattgg aaagattcag tcagacccag aattagaaga
     2401 acaattgcga cggttaaaat ttgatattcg caagaatggt gtcaaagtcg ctggcgatcc
     2461 atatttaatg aatgaggaga cggatgctcg aattgaaata aatcaagtaa ttgcagaatt
     2521 tgagcggatt gcggaccaag caaaagaacc agatattcgt caatacttgt tagaaatgaa
     2581 agcagagttg gaagtaaatg gtattacaga tgagtaacct aaaccaacac aactgactgt
     2641 taggcgctga ccgcggagga ttcgcgaagc agaaatcctc cgtggtgctg tgcctgttca
     2701 tggacggcgt tagccgacca tgatgtctca cccccgtatc taacaggggg gtacaaaaac
     2761 acaaaaaata agtaagcaac cattaagaat gcttgttgta tcaaagatag taagtaattt
     2821 gtataggtgt taactcattt gaaaaagtta acaccttgaa tttggaggtg agaacttggc
     2881 acaacggaat ttagattatc ggctattaaa agaccgacgc aacgaatacg gcgtttcaca
     2941 aaataaattg gctacggctt gtggacttag tcggccgtac ttaaatcaga tagaaaatgg
     3001 tggcgtaacc gcatctataa aaaccatgag gaaaattttt gaccaactgg aaagctttaa
     3061 tcccgatttg cctttgacgt tactgtttga ctatgtaagg attcgctttc ccacaacgga
     3121 tgcacgaaaa attattcaag aaattctcca tttgaaattt gagtatatgc tccatgaaga
     3181 ttatgccttt tactcctatc aagaacagta tgtcatgggt gatattgtgg tgatgttgtc
     3241 tcatgaagaa gataaaggtg tgcttttaga attaaagggg cgaggttgcc gacaatttga
     3301 aactttttta ctcgctcaaa agcgtagctg gtacgacttt ttcgaggatt gtctaaaggc
     3361 cggtggcgtg atgaaacgct tggacttggc aatcaatgat cgagtggggc tattggatat
     3421 acctgagcta acgaaaaaat gtcagaagga agaatgtatc tccttgtttc gtacctttaa
     3481 aagttatcgt tccggggaac ttttaaaggc tgatgaaaag gacggcatgg gcaatacctt
     3541 atatatcggc agtctcaaga gtgaggttta tttctgtttg tatgaaaaag actatgaaca
     3601 atatatcaag ttagggattc cattagacca aactgagacg aaaaatcgtt ttgagattcg
     3661 actgaaaaat gaccgtgctt ttcatgcgat tcaagatttg ttaaaaggtc gtagcattga
     3721 gagcaccacg ttttccatta tcaatcgtta cttgcgattt gcggataaag tagaaggtaa
     3781 aaggcgaacc aactggccct tgaatgaaca atgggggcgt tttattggac ggaatcggaa
     3841 agagattcaa ttaacttccg agccaaaacc gtataccatt gaacgaacct tgaattggct
     3901 aggccgacaa gtcgccccta cgtggaaaat ggctaaggag ttggatcgat tgaatcggac
     3961 aacctatatc caggatatgg tacagaatgc acgcttatca gaccgacata aaaagatttt
     4021 ggagcaacaa agtatggcaa ttgaaaattt gattatatga aaagaggaat cgtcatggga
     4081 attgtaaaag atattttttt ggtatttggt ggcagtatgc ttggtgttac tgtgatgtgc
     4141 ttgatgaatg caagtgcagc agcggatcga gcaatggaaa agaaaggaaa gtgacaagat
     4201 gaatttcgga caaaattt
//
LOCUS       DACKAC010000117         4215 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.121, whole genome shotgun sequence.
ACCESSION   DACKAC010000117 DACKAC010000000
VERSION     DACKAC010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.121"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            624..3263
                     /locus_tag="I0B46_002660"
     CDS             624..3263
                     /locus_tag="I0B46_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAR2084900.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(3431..4129)
                     /locus_tag="I0B46_002661"
     CDS             complement(3431..4129)
                     /locus_tag="I0B46_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAR2084901.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIAAILLIMIPI
                     ILFATTIYDGSASVSDATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVIGLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
BASE COUNT         1278 a          805 c          911 g         1221 t
ORIGIN      
        1 ttctttctgt tctgtccaaa ttattctaag atttctcttt accaacatca tttgacaaag
       61 agccagaaaa agccgagctc tcgataagct cggctttctt gtacctcgga aggattcaaa
      121 taaacagaaa tgattgtaac tttccatcat acatgcaaag cacggtgttg aatttcttca
      181 tgttaaacaa aaattttgtt ttcgttacat cttcaacatt tgaaacatct cttgttccta
      241 ttcaattttc agacgtttac gttattcaat tgttacgtat cgcattactt cttgtttcgg
      301 atacttcgtg atccaaacat ccatacgcat tcactattta taggtttgta ctctacacgc
      361 atgtctgggt gaaacctgta cttctaacaa ctttatttgc ttacactgtt ctatcgagaa
      421 attcttctgt ctatttgatg gtatcctcct tccttacatc tatataatag catcctttgg
      481 tagcgtttgc aaatataagc actttaaatt ttacgctttc ttaacaaata atcgataagc
      541 cctttatttg aagtaaataa aaaaaggatg ttatagtaaa ataagtacct ttttttgtac
      601 gaagatttta aaggaggaat aggatggaag aatacaagca gacaatagaa gaagtaaaga
      661 aggtaacaga agcagatcca gatactgggt tgtcttcaca agaagtggat gaacggcgaa
      721 aaaagcaggg attgaataaa ttcgatgagg cgccgaaaga atcgatgatc aaaaaattct
      781 ttcgaagctt atccgatttt acaacgatca tccttcttgt agcagcggtg atttcctttt
      841 ataccgcatt tgctacagaa catggggatt tatttgaagg actgctgatc atcgctatcg
      901 ttgtgatcaa ctcggtattg gcgattgtcc aagaaggaaa cgcagaaaaa gcactggaat
      961 ccttgcaaga tatgaataaa caaacggcaa ctgtcatacg agacggcaaa gtgaccactg
     1021 ttgagtcaga gcaactggta gtgggtgata ttcttgtttt agagtccggt gatgctattt
     1081 ctgccgatgc ccgtttgatc gaagcatcac aattacgtgt agaagaatcg gcgttgactg
     1141 gagaaagtga agcagttgaa aaagatgcag catttattgc tgaagaagat gagtcattag
     1201 gtgaccaact taatatggta tttaaaggat gtacggttgc tgcaggtcgt gggaaagcga
     1261 ttgtcactgc cattggtatg gctacagaaa tgggtaagat cgcggatctt ttgaacgaaa
     1321 atactatgca aaaaacacct ttacaggtcc gattgaatca gctaggcaaa cgaatcagta
     1381 tgatcgcttt agcggctgcg gctttagtat ttgtgattgg tgaactgcaa ggagaaccac
     1441 tccttgagat gttcatgact tcgatttcct tggctgttgc agctgtaccg gaaaccttga
     1501 ctgttattgt tacgttgaca cttgcatacg gtgtgcagaa gatggcgagg aaacatgcga
     1561 tcatccgaca gttgccagca gttgaaacgc taggaaccgc taatgttatc tgctcagaca
     1621 aaacaggaac gctgacacaa aatgaaatgc gcgtccgccg ggtatggagc aaagaagatg
     1681 aagtaacgaa tatcgaagat tcaatgacga acagcgcgat ggaaatattg aaaatggctg
     1741 cactatgtac agatgtgacg gtcgagcaag aagatgacga ccttgttatc aaaggtaatc
     1801 caacagaagt agccatcgta agggctgttg aagagaatta tcatacaaaa gcagaattag
     1861 aagaaaaata tccacgagta aacgagcttc cttttgattc tgaacgaaaa atgatgacaa
     1921 cagttcacca aatgggcgaa aaatacatct caatcaccaa aggagcattt gatgttcttg
     1981 ctccgcgctt tagttcaggc gatgtagaac aagcggcgat cgttaatgat cgctttggta
     2041 aacgggcact gagagtgatt gctgttggtt atgctactta tgacgaagaa ccgcaagata
     2101 tttcttctga agcactggaa aaagacttac gtttgattgg tctgatcgga atgatcgatc
     2161 caccacgtcc agaaagtaaa ggagcaatca agcgagcgaa aaaggctggc atcaaaacag
     2221 tcatgatcac tggagatcat gtggtaactg ccagtgccat agcgaaagaa ttaggtatct
     2281 tgaatgatcc gtcagaagca ctctcaggat cagaactgca tcaattatca gacgaagaat
     2341 tagatgcgcg tgtcaaagct ctctctgtct atgctcgggt aacgccagaa gacaagatcc
     2401 ggatcgtcaa atcttggcaa cgtacgggtg cagtcgtagc gatgactggg gatggtgtga
     2461 atgatgcacc agcgttgaaa gcaagtaacg ttgggtgtgc catggggatc acagggacag
     2521 atgtggcgca aagtgccgca gatatgattt tgacggatga caactttgcg acgatcgtag
     2581 atgctgttag tcaagggcgt tcagtttatc agaatattcg taaagcaatc aactttttac
     2641 taagctgtaa tatttcggag atctttattg ttttgattgc gatgctgtta ggatggggag
     2701 caccatttac agctgtgcaa ctactgtttg tcaatgtagt tgctgatgga cttccaggat
     2761 ttgctttagg aagagagcca gcggaacacg gcatcatgga tcagccaccc attccgaaaa
     2821 atgaagggat ctttgctcga ggtttattgc aaaaaattgc cataaatgca ggggtcttca
     2881 ccatcgtaac cttgtttggt tattatttag gtagttatgt cgatactatc agcccatggg
     2941 tagacgcaag tcagcatgtc ggtcaaactg ttgcattctt gatcttagct tattcatcga
     3001 ttctgcatgt gttcaatgta cgcagcagcc aatcgatctt caaagtcaat ttagcaacca
     3061 ataaagcttt ggtagaaatg gcgttactgg cattagctat tacaacggct gttgctttgc
     3121 tgccatttac gcaagaacta ttcggcctag tacacatcag tttgaatcac tggttcttgg
     3181 tagggatttt atctatcgta ccaatcgcgg tgaatgaact gatcaagttc catcgattac
     3241 cggaagcaga agaagaggaa tgattaactg tttctaagtt gattttcttt ttactggaac
     3301 tatacaaaaa ggaggtgtga catgaatgtt gtcacacctc ctttttgcta atgggctgaa
     3361 tgtttctttt actgcctctc gataaacggt gttctcctac caacccctcg gtcacaagtc
     3421 acactctctt ttaaatatct tttggcaatt gttcataaaa tgctgctttt gttgcggaaa
     3481 tgtatgggct gagccataga taaccaatac cagcagttgc taatgctaag atatgccagc
     3541 cgataaatga aagatctaac cagaaaagtt gtcctttata accatccatc aattttcgac
     3601 ttgcagtgat cgtatccagt actttaggtg tttgtcctgt ttcttggacg atatcgtagt
     3661 agataaaata gctttgagaa taagaatagc ttttaatgat tccaggaata actaatagca
     3721 aaccccataa tgttgtaaag atcgagatca gcacagctaa gccgatcact ccgccgataa
     3781 acaaaccttt gaatccacga aatgcatctt tgaatggttc gatctgatct ttttgtccac
     3841 gtaaaatatc aagataagtc caagaaatac cgctcatgaa caaagcgcta atgatgctgg
     3901 aaataattcc gccaccgcct cctgaactac cgcctcctga agttgtcgca tctgagacgc
     3961 tcgctgatcc atcatatatg gtggtggcaa ataagataat aggaatcata ataagaagaa
     4021 tagcagcaat accaatcaat gtagggatta gatttagtaa aacagcttgt ccccaacgtc
     4081 cttttagact ctcttttgct tggcgtttta attctgcaga agattgcatg atttttccct
     4141 ccttttcttt aataacacca atataccatg tatcaaaaaa tatttctgtc ataatgtttt
     4201 tctctttttt gttat
//
LOCUS       DACKAC010000118         4201 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.122, whole genome shotgun sequence.
ACCESSION   DACKAC010000118 DACKAC010000000
VERSION     DACKAC010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.122"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(3..1223)
                     /locus_tag="I0B46_002662"
     CDS             complement(3..1223)
                     /locus_tag="I0B46_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAR2084902.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1243..1848)
                     /locus_tag="I0B46_002663"
     CDS             complement(1243..1848)
                     /locus_tag="I0B46_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005234653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAR2084903.1"
                     /translation="MKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELPLF
                     NKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVDKP
                     VMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLIDEE
                     QVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1897..2829)
                     /locus_tag="I0B46_002664"
     CDS             complement(1897..2829)
                     /locus_tag="I0B46_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAR2084904.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLSNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3254..4111)
                     /locus_tag="I0B46_002665"
     CDS             complement(3254..4111)
                     /locus_tag="I0B46_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAR2084905.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKNDTID"
BASE COUNT         1192 a          849 c          692 g         1468 t
ORIGIN      
        1 ttttatttta cagaagcact cgaaactgca tctactgcag tttcatcgtg tgtgctggcg
       61 ctggttactg catctgtttt tccaccatga acagatgcac ttgaaaccgc atcttcattt
      121 ccaacaagtt gttgacccgt ttgttgtaaa taccactcaa tgattggttt gaaatcaaga
      181 atgtattcat tgattccagc atactctcca ttttcatcat gcattgcttg gtaattatgc
      241 acaatatatt tttctggcac ttgttttggt acatgaacac gtaccgcatc tgttgctccg
      301 cttcgcagtt gttgaattac ccactctaca tttttatacg atttttctgg gtgacagttg
      361 gctaatggat tccctacctg accgggcaca cgagcagcta acatctcttt cgcttcttgg
      421 cgcaaattat aatataaaaa ttggttatta ttatctgcaa aagttaattc catcggcatc
      481 gaatctaaaa agtaattgat ttgatccact gtcaataacc cacgatttaa tttgacgtaa
      541 gtatttccag aaacggcacc gactgcttca gaagcttttt ccaaccaatg atcatctgac
      601 atatcaacat cgggtattgt cgtagcaatt gttcctgtcg catgcaaatc ttctggtgga
      661 agctttttag gccctgctaa ggtagacacg actttaatga attttacaaa acttgctaat
      721 gtactgccta aaaagtcaac agttcgtgga tcaatcaagc gatcttcttg gtcaaatgct
      781 tcttttgctt ttcccaataa aaattcattt ccgggcatca ctaatgcatt cacgcctggt
      841 gcgtccaaaa tttggcgcaa gtgtaattgc gcgcgtgaag ttccttgatc gtagtaagaa
      901 gcacccacaa ccattacagg tttattttca agtggatgga ttttaaatga tagccattca
      961 agtgcacttt tcaatcctgc tggaaccgta tgattgtgtt ctggcgtagc aataattacc
     1021 ccatcagcca ttaagatttt gcgatttagc tgttgaatag caggactatt tgtttgatca
     1081 tcatcttgat tgaacagagg taaatctttg atttcaatga tttctaaatt aaatagttca
     1141 tgatattgtt tttggataaa tgttaataat ttacggttat atgaaagttc agctgctgat
     1201 ccgacgatac ctattaattt catctttgtt tgctcccctc gattaaatcg tttcccatga
     1261 aaagctttcg gcttcacgtt tatttgcttg gtgcgcatgt tgcaattgac tagtgattgt
     1321 gacaaagagt aagaaatctt tgaatagttc acgtaatttt gcgacttgtt cttcatcaat
     1381 gagtcgtcct tgctcatcaa aggcttggag tgaatgaccc aataaaaatt ctgagcttgg
     1441 cataattcgt gcctttaact ctggtgaatc caatatttgg cgcaaatgtg cttgtgcgcg
     1501 tgaagaacct aacgtgccat aagacgctcc ggtaatcatc actggtttat ccacaaacgg
     1561 aaaaatacca tatgaaagcc aatttaatgc gttaatcaat gaagcaggaa ccgcatggtc
     1621 atactctggt gtgctgatga ttacgccatc tgcttgttca atttttccag caatttcttt
     1681 aacaacattt ggtagttctg tgttttcagg tttgttaaat agtggcagct ccttgatttc
     1741 gacaatttcg atttctgcct catttacaaa ataatttttc ataaactgta ataattcacg
     1801 gtttgttgac cgcgttgaat ttgttcctac taatccaata aatttcatct tcattctctt
     1861 tcctttctaa catcgatttc tgactgttcg cacgtactaa attttttcaa atccttttgt
     1921 atacaagaat tgattctctt ttgttacaac tgctccctcg atacccggct gttcttcaag
     1981 cgctttcaac acttctaaag aagatgctcc aaataaacgt gtagtccaga tttctccatc
     2041 tacagaagaa tcagacaaaa tcgtcaggct tgctacgtca tttttaactg gaaagccagt
     2101 tttcggatca atgatgtgat gatacactga cgaaccattt tgtagataac gttcgtagat
     2161 tccagaggta actaaagatt ggttgcttag tttaaccacc gcaagcgact cttcacgtga
     2221 acctttagga tttcgcaaac caatacgcca agcaccatcc gaacgtcttg gtgattcacc
     2281 aaaaacaacg atatttcctc ctaagttaac taaagcaaac gctgcatttc gctctcggca
     2341 atgtgtaatt aatttatctg caatgtaacc ttttgctaac gcccccaagt caatcatcat
     2401 tcctggtttg gttaaaaaaa cggttttcat tttttcatct aattgaattt ggtgtggatc
     2461 cgttagcaga agtaactcag ttatctctgc ttttgttgga accaatgcat cttcaaatcc
     2521 aatgtgccat ttttgtacaa ccggaccaat agcaatattc agataactat ttcttgcaca
     2581 actatgtgct tttcctaatt tgatgagctc aaaaagttca ggatgtacaa tcacagcttg
     2641 tcgtccagca gcttgattga tggccattaa ttcagaagtt gcatcatgtg cgctgaagcg
     2701 atgttcataa acgtgcaacc attcgcctaa ctctgctagt aatcgactag gctcttcatg
     2761 cccaacttgt aatgtaattg ttgtccccat catttgccag attttcgttt cttcgatcac
     2821 ctgattcatt ttgtaccttc tttacttgaa ctacggatta catgagtaat tagtttttat
     2881 ttttgttact gttttcacaa ccagatgata cgcctgctag atttatttgt caaccgattt
     2941 tatatatttt tttatataat aaggtaattt taatataaaa ttttatattg taaaaaaaca
     3001 aataagggaa acaaacaaca catttttgtg tgttgcttat ttccctctaa ctatcgtttt
     3061 ctatttgatg gttaacctac ttaaaataaa tactgcaacg acaaacggag atcaagcatg
     3121 ctttgtaacg tattttacga gaattacgac tttattttat tactctatct tgctgccata
     3181 cccgtctatc cctaatcatc tttggtgaat tttttcaaag ttagcataac aaaaataagt
     3241 attttttcct tcgttaatca atagtatcat tttttttcat atgatttaat tcatcaatta
     3301 attttttgtt tttattaatt ttagcaaaag ctagtgcatc ttgaatcaat tcgagcactt
     3361 ctgccttcga cttattttta gcctgtgcgt tcttcgctaa ttgggcagca gctcgtgcta
     3421 agtaataagt caaatggtta tccgaacaaa tatcgattgc gtaatttagt agtgcatcac
     3481 ttgtttccaa atcaccgtct tgcgcaaaaa aaacaccaca atgaaagacg atatttaaca
     3541 cccgccagat atcttctgtt gtacgaatcg gatacgtata aattttttca aacactcgat
     3601 tgaaataaac ttctgctttt tccaactctt tttctctagc atagaccatt ccaatacctg
     3661 tatatgccaa taaacgaaac aacgtgttgg ctgtttctgc catcaacaac tgatcaaaat
     3721 ggaacaacac atccgtaatt ggtgcattcg taaaaatcat ttcaaatcct tttagatagt
     3781 agtaacgtgc aatcagctca gactcgtcaa ttaccttggg atcaatggct gctaaaatct
     3841 gtgtcaattg ttcatattca ctcgtaatca gtgaaaactc tgctttttct agctgttcat
     3901 caagttgtgt attgatcacg ttcatcgaag aaaaaagttc aactaacgga agattgattc
     3961 gattacataa tttgattaaa atttttaacg aagggacttg accattgttc tcaaatttac
     4021 ttaatgttgc ttgtgtacaa attccttcag acacttcttt ttgagataag ttggcagcct
     4081 ttcttgccgt aataaatttt tcaatgttca ttattttctc tccaatcaga cctcacacga
     4141 aaatcatttc tgtctcgatt ttagcatgtt ttctttggga atgctttaat tggttaagat
     4201 a
//
LOCUS       DACKAC010000119         4174 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.123, whole genome shotgun sequence.
ACCESSION   DACKAC010000119 DACKAC010000000
VERSION     DACKAC010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.123"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            612..1424
                     /locus_tag="I0B46_002666"
     CDS             612..1424
                     /locus_tag="I0B46_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084906.1"
                     /translation="MILLAVSMSLLIYTHILSTFMSVIVIGFLFIGSLFSKRKERKEM
                     SNLISNMIKAVGWTILLTAYVWYPMLQQVLHQKINRPFRTKLQERALNISDSLIGAVN
                     NDLVTFTMGTIGVVSFILPLFFKKKFANKEKIINFCAVTTWFLSTNLFPWEFLQKTPI
                     QIIQFPWRILGFQVLFGSLILIIVFLKWKTSNKKSMCSLVGIVLLIFTVTVATEANYS
                     QKIQSYKGRLIMTKKDVTLYTTSRTGGLYDYAPLDALKYKDHLKKHEVKVYD"
     gene            3059..3613
                     /locus_tag="I0B46_002667"
     CDS             3059..3613
                     /locus_tag="I0B46_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAR2084907.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
     gene            3867..>4174
                     /locus_tag="I0B46_002668"
     CDS             3867..>4174
                     /locus_tag="I0B46_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084908.1"
                     /translation="MINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAINNGNLPSN
                     KKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIG
                     "
BASE COUNT         1542 a          516 c          660 g         1456 t
ORIGIN      
        1 atggcctttt tacttttaca caattataag gactttatcg aaaaaaagca gttatttgtt
       61 aaaatcattg atattattaa taataagtat tttttgaaag gggaaatata aaaggttttt
      121 gaaaataaat aaaaaatagt attacttgtt ctattgctag ttattgctag tctttattct
      181 tttttgtttt ttagggatgt acaaaagatc tcaaccacag gagacattgc ttttcacttt
      241 tctagaataa aatgcttgtc ctcagtattt tctggtccaa taaattttac cacatttaat
      301 agctatggcg gaggcgtgaa tttttttatc catatctaac atctttccag ccgtaatatt
      361 ttactggaca acaaacaatt tgattctaag ttatatactt actatacgtt gggatactga
      421 atatctgtac aattctagtg agctatcaat atggacaaaa gaatagatgc agccttctta
      481 ttcagctgtt tgtatagctt ttatggattg atatatatta gcgtactgca atttcagaat
      541 caattgcgtt gacgattgta ccagttgtct tgtattacac atatactctt ctttatgaaa
      601 gaaaaaactg cgtgattctt ttagctgtca gtatgagttt gctcatctat acccatattc
      661 tctccacctt tatgagtgtg attgttattg gcttcttgtt tatcggctct cttttctcta
      721 agcggaaaga gagaaaagaa atgtcgaatc tgatatcaaa tatgataaaa gctgttggtt
      781 ggaccattct tttaacagct tacgtttggt atccgatgtt acaacaagtc ctacatcaaa
      841 aaattaatcg tccttttcgc acaaaacttc aagaaagggc attaaacatt tctgatagtt
      901 taatcggtgc agtaaataat gatttagtta catttacaat gggaacaatt ggtgtggttt
      961 catttattct tccacttttc tttaaaaaaa aatttgccaa taaagaaaag attattaatt
     1021 tttgtgcagt gaccacttgg ttcttatcaa cgaatctttt tccatgggaa tttttacaaa
     1081 aaacacctat tcaaatcatt cagtttcctt ggagaatctt gggttttcaa gtgctttttg
     1141 gttcattgat cttaattata gtgtttttaa agtggaaaac aagcaacaaa aaaagtatgt
     1201 gtagccttgt aggaatagtc cttcttattt ttacagtgac tgtagcaaca gaagcgaatt
     1261 attcgcagaa aattcaatcg tataaaggtc gtttaattat gaccaaaaaa gatgttacac
     1321 tttatacaac ctcgagaact ggaggtcttt atgactatgc accattagat gcattgaaat
     1381 ataaagatca tttaaagaaa catgaagtaa aagtctatga ttgatgggag aatgcatctc
     1441 ataaagcaca taattctaaa ataatctata cagtaggaga tgcgaatgag caaaatatca
     1501 cgttacctgt atttaattac tgggaacagt tatgaaagta aatgggaaaa agagcactat
     1561 agaaaatgac aatggtttag tgtctattaa aggtacggga aaaagaacta tagttgaggt
     1621 ttcatctacg tatccaacta cgatgattct atctttatta attagttcaa taacttttat
     1681 attcttattt acacgttatt taataagatg ctgcggttaa atgaaaatag attagataga
     1741 tattataaat atgttgattc tattttcata gcaatggaga aaatacatta gcaatatata
     1801 aaagaaggta tcaagagtca cggtgaattt attccgaaaa gtgaaaagaa caaatataaa
     1861 atttaataaa aatatagaac taaataggaa aacgtagacg taattttcta cgttttcctt
     1921 ttcatatata ttaaatatta cggaaattta tataaactaa tttagtgttt tgtttgtaat
     1981 tcctaatttt gctccacttt tagataattt ttaggtctat ttaactcatc agacattcta
     2041 tgctcatttt ctccaaattt gtttcaactc tttcgtactt aattttgcgt atttgaaaat
     2101 tcttctttta gagtactttt attacgatac tagtatgata tatatctata gcaaacagtt
     2161 aaaatcttcc taaaataagc gaatagcatt cctaaaacct ataaaatttt tagatgaagc
     2221 gacaagtgga caattaataa actacttgaa catgaaatta tggatcacct ttcaaattta
     2281 gaaaatggaa ttcttatact tattgcgcaa catttattga ttgcaaaatt atgtgacaaa
     2341 aagaactagt agaagaagga ataatggctt gataataaag tactacctat taaaggtagt
     2401 actttattta atttagtata aattaacata ctactatatt ttaagtttca tattatataa
     2461 attaataata tactaccatt ttttggtagt ataatttaaa ggtaataagt tattctttta
     2521 ttactatgtt tgggttttat agcaaggaaa gtttaataag agtttggacc tagcaaacaa
     2581 tattaatgag gtcattaaat cttattatct ttagtgatat aacgaaatat ctacacagac
     2641 aaaattatga tcttgcgtaa agttataaaa tattaataaa aatattttat taatgaaaag
     2701 cttggtagca cggttattca tatgctaatt tatttcactt tatagtaagg ggagttctta
     2761 gtatattgtc caaatttaaa tttcatataa tttaattatg taaattagct attctaagaa
     2821 cttctacgga taactgcccg tctaaaattt aattaagagc ttagaatctt catataagta
     2881 aggttaattt ttttgtacaa aatagtcgga atattctgag ttactgaaat gatataaaat
     2941 cataaactaa accccttgat tttcttcact tttaacagag tatatatata agttcatgat
     3001 tatggtaaaa tatatatata atcatgaact cagaatattt ttaagaaggg aaacaatagt
     3061 gagctataat gtacaaccgt taaggacaaa tgaagagata caagattttc agttttggct
     3121 aagaagaaca agtaatccag aaagagatag ttttttattt ttgtttggaa tcaataatgg
     3181 gttacgtatg tcagatatta taggattaaa agttggagac atacgaggta aaagtaagcc
     3241 tattattgtt gaacataaaa caggtaagcg aaaaccgatt tttatagata atttgagaga
     3301 agagatttta ttatatactg aaggtaaaga agaaaatgat tggttatttc caagtcggca
     3361 acaaggtcga catataacaa gggatcgtgt ctatcagata tatgcagata tagcagaaaa
     3421 attaggaaga gatgatatag gaacacatac attaagaaaa acttttgggt atcactacta
     3481 caagaaaact agagacattg ctaccttaat gtttattttt aatcatagct cgcaagcaat
     3541 tacaaaaagg tacattggta taactgaaga tgaaataggg gcatcgttac gtggttttaa
     3601 attgggaggc taattgagat attaggatat attttttgtg tataatgaaa acttagaagg
     3661 tgagattaat gaagataact gaattttcgg aagtatttaa aacgtttagt aacatttaca
     3721 atagaatacc agagatagaa caaaatgttg tagttgataa tgattattat atagagaatg
     3781 attttaataa accaagaaaa tatgacaacc tatacaattt tatacacaat agaacaacaa
     3841 taaataacaa cagtaacact cgagttttga ttaattcagc atattttcat attaaatatt
     3901 taaagaaata tgagtatgaa gaagttgtat tagattatgg gctaaataga cagtcatttt
     3961 taatttatgc aataaataac ggaaatttgc caagcaataa aaagaacata attattgaaa
     4021 catatgccta tctggagggg ggatctaaaa aactaattaa gtcatgtaat atagagtatc
     4081 aattatcagt aggagaaatt aaatgtgttt tcgaagaaga tttagaactt gacttaaaaa
     4141 aaatttttgc cagcattcat gattatatag gttt
//
LOCUS       DACKAC010000120         4063 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.124, whole genome shotgun sequence.
ACCESSION   DACKAC010000120 DACKAC010000000
VERSION     DACKAC010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.124"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            74..337
                     /locus_tag="I0B46_002669"
     CDS             74..337
                     /locus_tag="I0B46_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HAR2084909.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            331..681
                     /locus_tag="I0B46_002670"
     CDS             331..681
                     /locus_tag="I0B46_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAR2084910.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(705..1319)
                     /locus_tag="I0B46_002671"
     CDS             complement(705..1319)
                     /locus_tag="I0B46_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAR2084911.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            1768..3093
                     /locus_tag="I0B46_002672"
     CDS             1768..3093
                     /locus_tag="I0B46_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAR2084912.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTERTRSQRRKKLAQMIQEKIKEELGLIA
                     TVGIGDNPLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYSIKSRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRNVGITYSKLVYTDSLQLDLFSDPQKQIDEAN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            3086..3436
                     /locus_tag="I0B46_002673"
     CDS             3086..3436
                     /locus_tag="I0B46_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084913.1"
                     /translation="MINYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EISEVLNAAVIKNRLVCIQKEERNAEGYYPDDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            3433..3612
                     /locus_tag="I0B46_002674"
     CDS             3433..3612
                     /locus_tag="I0B46_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084914.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            3748..4041
                     /locus_tag="I0B46_002675"
     CDS             3748..4041
                     /locus_tag="I0B46_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAR2084915.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFIYDLSLASSCNMSDVYEKLLILENEYDQFNSNQKKKKKKHID"
BASE COUNT         1415 a          623 c          792 g         1233 t
ORIGIN      
        1 aaaaggataa ttaagtatat acgttttttc ttatgtatgt tatactatgt atatacagaa
       61 agaggtgtct actatggaaa tgttacctat aaaaaaatgg ggaaatagta atggccttag
      121 attgccaaag tatattatgg aatatcttgg aattcataca gaagacaaag tgaagataat
      181 acaagaagaa gtaaacggtc agaagcggct aattatcgaa gcggctaata tagaaaatga
      241 attaacaatt gaacagttat ttgaaaatta cactgatgaa agaaatcatg tgacgattca
      301 aaatttaggt gaagcagtag gtaatgaaaa atggtgagaa aacctagaca aggcgatatt
      361 ctattattga atacagcacc tcgatctgga cacgaacaga caggtaaaag accttatatt
      421 gttttaagtc atgatattat tgcggattat agtaacgttg ttacagttgc tccaatttct
      481 agtacaacta gaaactatcc gctatatgta aatataaact cagaatatga aatgaagaca
      541 acaggaaaag tattattaga tcaactaacg acaattgatt atgaagccag agagtgtatt
      601 tttttggaaa aagcgcatga tagtttagtt gaggagttat tgatgaaagt tagaacagtt
      661 tttcaaaaag taagcaaata aaatagaaac agcagcatga tcaactattc gtgattgacc
      721 atgctgtctt tttttattct atatacggtt tgtctcgtaa cattgtagtc ttttgcaatc
      781 ttagcgatcg caacaccttt ttttagatcc tcaacgatgt ttttataaac aagacgacgt
      841 tggggatctt ttgcattggc actgtatagc ttaggacgtc ctttataaac accgttcgct
      901 tttgcaatct taatgccttg tgcctgacgt cgtttagatt cggtacgttc ttgttcagcg
      961 atcatggcca aaatttgaat gatcaaatct ttaatgaatt tatccaggag aggattgcca
     1021 attgcttcgg ccatgatcgg tagacttgtg atgatcagct gcacattttt cttcttcaag
     1081 taattgactg aagcgatgat ctcgtcgtaa tttcttccaa gtcgatcaat agcttccaca
     1141 ataaaaatat cttgatctcg gacgaaatcc agtgcttctt gaaaaacagg tcgatgagta
     1201 atcgttgcgc cagattgttt ctcaatgaaa attctctctg caccgaattt tttcatttct
     1261 tcaatctgcc gttgttcatt ttgatcgatg gatgagacac gtatgtatgc aactctcaaa
     1321 atttttacct actttctgca actatttaaa aaacacccta atcatacacg agaaaaccag
     1381 atttttcaat gtcgcggtcg tgtctagtta gggtatacct tatatagaca aaaactaatg
     1441 atggaggaaa tagtatgaga attttttgta agtgtctact gataacttat tctcaaatgt
     1501 tgtaaggatt ttattcattt gttaatatta aagaacggaa ctccaaaaaa gtccgtactc
     1561 taatttgcgg tctctcactc ttttagagtg agagttttta ttagattttc aaattaatta
     1621 gtaaagtgat agaataaatt gatcatacaa atcaaaagaa gaagcagggt gatagcatac
     1681 ctatgcttct tttttatgta tttagctatt tcttctttac aatatcgaac atatgtttgt
     1741 ataatgtgaa agaatggagt gttatttatg atggattatt caaaagagcc agtaaatgat
     1801 tactttttaa tcgatatgaa aagcttttac gcaagtgtcg aatgtataga aagaaattta
     1861 gatccattaa caacagaact tgttgttatg agtcgagctg ataatactgg ttcaggattg
     1921 atattagctt cttctcctga agcaaaaaag cggtatggta ttacaaatgt gagtagacca
     1981 cgtgacttac cacaaccatt tcctaaaaca ctacacgttg ttccaccacg tatgaaccta
     2041 tatatcaagc gaaatatgca ggtaaataat attttcagaa ggtatgtagc agacgaagat
     2101 ctattgatct attcaatcga tgaatcaatc ttaaaagtga ccagatcact gaaccttttt
     2161 acgaccgaaa gaacacgaag tcaacggaga aaaaaacttg ctcaaatgat ccaggagaag
     2221 attaaagaag agctaggttt gattgctacg gtaggcatcg gcgataatcc ccttttggct
     2281 aaattagcct tagacaatga agcaaagcat aatgaaggat ttattgcgga atggacgtac
     2341 gaaaacgttc ctgagaaagt atggaatatt cccgaaatga cggacttttg gggcattggt
     2401 tcgcgaatga aaaaaaggct caaccaaatg gggattatga gtattagaga tcttgctaac
     2461 tggaatcctt actcgatcaa aagtcgttta ggtgtaattg gcttgcagct ttattttcat
     2521 gcaaatggta ttgatcgaac tgacatagca atcccaccag aaccgacaaa ggaaaaatca
     2581 tatggaaatt cacaagtgtt accacgagat tatactagaa gaaatgaaat cgagttggtg
     2641 gtaaaagaaa tggcggaaca agtagcgata cgcattaggc agcataactg taaaacaggt
     2701 tgtgtccatt tacacattgg cacgtcgatc ttagaaacga gaccagggtt ttcacaccag
     2761 atgaagatcc ctataacaga taatacaaag gaattgcaga attattgtct tttcttgttt
     2821 gataagtatt atgaaggaca agaagtaagg aacgttggaa taacctatag caagcttgtg
     2881 tacaccgatt cgcttcagct agatttattt agtgatccac aaaagcagat tgatgaagcg
     2941 aacttggata aaattattga taaaatcaga caaaagtatg gttttacgtc gattgtacat
     3001 gcttcttcaa tgttagaagg cgctagaagc attactagaa gcacacttgt tggtggtcat
     3061 gcgggcggaa atgggggaat taaaaatgat taattacaat gatcgtggac gtttaaagtg
     3121 ggcagggttt tatttatctg atcatactga agaaattgat gccgacaatg aacaacgaac
     3181 cagtcaaaat ttagcaaaag aacaaatgac gactgaagag atttcggaag tattgaacgc
     3241 tgcagtcata aaaaatagat tagtatgtat tcaaaaagaa gaacgaaatg ctgaaggata
     3301 ctatccagac gatataattg gcaaaatatt aggtagtgat gagctgggga tctacgttgg
     3361 caaagacaaa attcactacg acgaaatacg tcatatcgaa ttttaccagg ataataaatg
     3421 gagtcgatta gaatgaacat tatccagcaa tatgagctaa agtatataac ctttgatcag
     3481 ctatcagaag agatttgggg ttatggccaa cgtttgataa atgaggttgg tgttgagcgc
     3541 ttctctttct atgtagaagc tgcagcaggt tatcataatt ttaggtttta tatcttcccg
     3601 ttatatatat aaggtatttt agggattcta tagagttatc tctatgaatc cttttttgtt
     3661 tttacaaata ggagtagata ctagaataat gcgtgtgtta aactattatt gcaaacgcaa
     3721 taataaaaga aagaaggctt acgcaccatg tctatcaata catttgttta ctcgcacccg
     3781 atcaatgttt acattatcaa gaatttagga atcactgtag aacaattttg tgagttatat
     3841 gcatacccac aaggaacggt tgcaagctgg attactcgac aacgtagaat caaatcacta
     3901 cctgcttcat ttatatatga tctgagtctt gcttctagtt gcaacatgtc tgatgtctat
     3961 gaaaaattat tgatactaga gaatgagtac gatcaattta attccaatca aaaaaagaaa
     4021 aagaaaaaac acatagatta atattgaaac gaaaaatagt att
//
LOCUS       DACKAC010000121         3956 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.126, whole genome shotgun sequence.
ACCESSION   DACKAC010000121 DACKAC010000000
VERSION     DACKAC010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.126"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..156
                     /locus_tag="I0B46_002676"
     CDS             <1..156
                     /locus_tag="I0B46_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HAR2084916.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
     gene            175..1452
                     /locus_tag="I0B46_002677"
     CDS             175..1452
                     /locus_tag="I0B46_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAR2084917.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1462..2118
                     /locus_tag="I0B46_002678"
     CDS             1462..2118
                     /locus_tag="I0B46_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAR2084918.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2118..3494
                     /locus_tag="I0B46_002679"
     CDS             2118..3494
                     /locus_tag="I0B46_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAR2084919.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            3624..>3956
                     /locus_tag="I0B46_002680"
     CDS             3624..>3956
                     /locus_tag="I0B46_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAR2084920.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDI"
BASE COUNT         1594 a          423 c          693 g         1246 t
ORIGIN      
        1 gataaggata agtgtattaa ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat
       61 tcagttgaga attgcaatca tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt
      121 ccagtggatg caattagtta tggagtaaga aaatagagag gagtagttag gaaagtgata
      181 aaaaatgcta tagcatatat tacaagaaag aaaaatagaa ctttaataat ttttatcata
      241 ttaactatag ttctttcttg cttgtattct tgtttaacaa taatgaaatc aagcaatgaa
      301 atagaaaagg ctttatatga aagttctaat tcttcaatat caataacaaa aaaagatggt
      361 aaatatttta atgttaatca atttaaagat attgaaaaat taaaagaaat tgaagaaaaa
      421 ataattcaat atgatggatt agcaaaatta aaagatgcta aagtagttag cggagaacaa
      481 agaataaata gagaagattt atctgacgaa tttaagaatg ttgtttcact cgaagctaca
      541 aataatacta aaagaaatat tttatttagt agtggagtat ttacaattaa agaaggtaaa
      601 aatatagaag aaaatgataa gaattcaatt attgttcatg aagaatttgc taaacaaaac
      661 aatctaaaat taggtgatga agttgatctt gaattactag atattgaaaa aagtggaaaa
      721 ataaaaagtc ataaatttaa aattataggg atcttttctg gtaaaaaaca ggaaacatat
      781 acaggattat cttctgattt tagtgaaaat atggtgtttg tagattattc aacaagccaa
      841 gagatattaa ataattcaga aaataatgaa attgcaaata aaattttaat gtattctggt
      901 agtgcagaat ctacagactt agccttaaac aaattgaaag agcttaaaat tgatgagtca
      961 aagtattttg ttgaaaaaga ttctaatgcg tttgaagagt ctttagagtc agtgagtgga
     1021 ataaaatata taataaaagt aatgacttat tctattatgt taggtgggat ggttgttctt
     1081 tcattaatct tgattctatg gttaagggaa agaatttatg aaataggtat attcttatct
     1141 attggaacat ctaagataca aattataatg caatttatat tcgagttaat attcatatcg
     1201 ataccaagta taatatcttc cttattttta ggaaatgtat tactaaaagt aattgtagat
     1261 ggattcatta actcagagga ttcaatgatt tccggtggaa gtttaataaa taatagtagt
     1321 tttatgtcaa atataacaac acttggacaa agttatttaa tattaataag tattattgtt
     1381 ttatcagttg tatttgcttc ttcattaatg ttaatcaaga agcctaaaga aatcttatca
     1441 aaaataagtt aggagaaaat aatggatata ttagaaataa agaatgtagc atatagttat
     1501 gcaaattcta aagaaaaagt tttgtcagga gtaaatcaaa aatttgaact tgggaagttt
     1561 tatgcgatag taggaaagtc aggaacagga aaatctacac ttctttcctt acttgctgga
     1621 cttgataaac ctcaaacagg aaaaatattg tttaagaatg aagatataca aaataaagga
     1681 tatagtaatc atagaaaaaa taatatatct ttagtatttc aaaactataa tttaatagat
     1741 tatttatcac caattgaaaa tattagatta gtaaataaat cagcagatga aagtatcttg
     1801 tttgaactag gtttagataa aaaacaaata aaaagaaatg ttatgaaatt atctggtgga
     1861 cagcaacaaa gagtagctat tgctagagca ttggtatcag atgctccaat aatactagct
     1921 gatgagccta ctggtaatct agacagtgtt actgctggag aaataattaa tatattaaag
     1981 acattagcta aagatagaaa taaatgtgta atagttgtaa cacatagtaa ggaagtagca
     2041 gattctgcgg atatcatttt agaactaagt ggtaaaaagt tgaaaaaagt aaataaaatg
     2101 aatttggagg ttgaataatg ataaaaaatg catttgctta tgtaactaga aaaagtttaa
     2161 aatcattaat tatcatatta gttattttat ctatgtcaac tttaagtctc attagtttat
     2221 ctattaaaga tgctacggac agagcttcaa aagaaacatt tgctaatata acaaatagtt
     2281 tttctatgga gataaataga caagtaaatc caggaacacc tagaggtggg ggaaatgtaa
     2341 aaggtgaaga tattaaaaag atatctcaaa cagatagtat agactcctat gtaaaaagaa
     2401 taaacagtgt tgcagattta gttgattatg atattattga aactcaagag actcttgcga
     2461 atcaatcacc tgaaagagca aagaatttta aaagaacagt tatgttaact ggggttaacg
     2521 actcaacaaa agaaacaaaa tttgtatcag aagcatataa attagtagaa ggaaagcatt
     2581 tagaaaatga agataaaaat aaaatcttaa tgcataaaga tttagctgaa aaaaataacc
     2641 ttaaagttgg agataagata aaaataaaat ctaatttatt tgatgctgat aatgagaaag
     2701 gtgcagatga aacagtagaa gtagaaatta aaggtctatt cgatgggcat aatagcggtg
     2761 tagtaagtgc agcacaagaa ctatacgaaa atacattaat cactgatatt catacagctg
     2821 ctaaagttta tggtaatacc gaagatacag ctgtatacca agatgcaaca ttctttgtaa
     2881 aaggtgataa gaatcttgat agtgtaataa aagatcttgg gaaattagat ataaattgga
     2941 gagaatataa tttgattaaa agttcatcaa attaccctgc attacaacaa tctatatcag
     3001 gtatttactc aatttcaaat aaattatttt ttggctcatt aatatttgca ggagttgtag
     3061 tttcattatt attattctta tggatgaatg ctagaaagaa agaaatagca gtattactat
     3121 cattaggtat atcaaaatta gaaatttttg gtcaattcct aattgagatg gtatttatat
     3181 caatccccgc attcgttggt tcctatttct tagctcaata tacagctgac aagcttggga
     3241 ataatatatt gaataaggtt actggtgata tagctaaaca aatagctaaa caatctgcat
     3301 ctagccaatt aggtggtggg gcagaagctg aaggatttaa caagacatta tcaagtttag
     3361 atatcaatgt attacctaaa ttcatgattt atgtagttat atttatgagt ttagtacttc
     3421 ttgtatcatt gattatttct tcattcaata tattaagaag gaatccaaaa gaattattaa
     3481 ttgacaataa ttaaaatttt cggtgctttt tagcaccaaa aatgagagtg taatataaac
     3541 cgtgtaagta accatcaatc ctaggcgggt ggttgcttca cggtttttat ttttctaaaa
     3601 taagatcaag aggtgattac acgatggcta gaaagaaaag aaatcctgat gctgagaagt
     3661 tagctgaatc cattttgaat acttatcaac ccgaatctgt agaggatatg caagatgttt
     3721 taaaagatgt gtttggtccc ttatttgaaa aaatgcttca aggagaattg aataaccatt
     3781 tagggtatga cgcccattct aaagagccaa agaaacatga caatcgacga aacggttatg
     3841 gcaataagac gcttaaaacg agctttggta aagtaaacat tgatgttccc agagaccggg
     3901 aggcttcttt tgaacctgag ttgattccta atagaaaacg agatgtctcc gacatc
//
LOCUS       DACKAC010000122         3883 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.127, whole genome shotgun sequence.
ACCESSION   DACKAC010000122 DACKAC010000000
VERSION     DACKAC010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3883
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.127"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            82..3123
                     /locus_tag="I0B46_002681"
     CDS             82..3123
                     /locus_tag="I0B46_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002386122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAR2084921.1"
                     /translation="MIHLVENKEIRFEQDIETSLTTYGKWKKETFNESNFDPIIGLDC
                     QKLLGFVQKTQPKQWARHQRNYNNDGEVKFLQRFNQEVETHGMLHVLRNGIKDRGVRF
                     KVAYFKPASELNDETMEKYEANSFTCTRQFAYSPNNHKTIDMVLSLNGIPLVALELKN
                     QYTGQTVENAKLQFQKDRDPKELVFRFNRRFLVYFAVDHTDVYMTTKLDGKKTYFLPF
                     NQGSNGPGRVGGAGNPSNKDGYPTAYLWEKVLTRDNLMDIIQRFMNLEIKKEKQGDRE
                     VTKKTLIFPRYHQLDVVRKLVADVKSHGSGENYLIQHSAGSGKSNSIAWLAYHLASIH
                     DQLNYPIFSSIIIVTDRTVLDRQLQNTIMNFDHQTGLVETIDDRKSSQDLKEAINDGR
                     KIIITTLQKFPVIYQEVDQVEGRNFAVIVDEAHSSQTGNSAQKLKAALSDKSKSLEEY
                     YELEGEVDDVLPDAEDELVQQLVAQGKHQNLSFFAFTATPKEKTLEMFGTKQPDGHFE
                     PFHVYSMRQAIEEGFILDVLKNYMTYSTSYKIAREIEDNPELPKSEATRAIVRFQSLH
                     PWAVTQKTAVMVEQFRAVTRTAIGGQAKAMVVTASRLHAVRYMKEFKKYIANKGYNDL
                     DVLVAFSGSVVDGETEYTETKMNVTKSGENVRENQLKEAFHTDNFNILIVAEKYQTGF
                     DEPLLHTMFVDKKLKGVKAVQTLSRLNRTMPGKKDTFILDFVNDVEDIEAAFQPFYEQ
                     TNLREEINVNLIYDTQSKLRKFNVYNQGDIERVMKLVKQAQKQQNERLLGRISSMFKP
                     IMDRYEEMNKDNQYQFRVTLRNFGKWYDYISQLDRLFDMELLEERIFTGYLLKFLPKE
                     TRENIDISDKVKLEYYKLRQDFSGEIHLVNKEGELVNPKSIDAGIKPPDERDSLEEII
                     HKINERFPNDFDEGDRVLVEILYRSFADNPDLKVVSMAKNNDAQMFEKSLFPDVFKDK
                     VMEQYEKNNAAFEKMFGHDDKYFDLVYTLVARDLYKLLRTK"
     gene            3313..>3883
                     /locus_tag="I0B46_002682"
     CDS             3313..>3883
                     /locus_tag="I0B46_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HAR2084922.1"
                     /translation="MSTTIEVNKQSVKQFLETGKIKKFVIPEYQRPYAWTDEQNQVLF
                     DDLAEYTANNNESTYFLGSIVAYENDHNEQEIIDGQQRITTLFLFLRAIYAKLENSCE
                     KEALFLKSQIEPALWEQDDLTGEVKPDKILIMSRVMGDEGNEEFASILVSGEADVKSK
                     SNYSKNYILIQHLLNEYATNEPLSFYRFIS"
BASE COUNT         1329 a          621 c          824 g         1109 t
ORIGIN      
        1 atagctgaat tgatggaact tgagagttat aaaaaagctc tcatctacga atacgtaacc
       61 ggtaaaaaag aagcctagga ggtgattcat ttggtagaga acaaagaaat tcgttttgag
      121 caggatattg agacttcact gactacgtat ggcaaatgga agaaagaaac attcaatgaa
      181 tcaaactttg atcccataat tggtttggat tgtcaaaaat tgctaggctt tgtccagaag
      241 actcaaccaa aacagtgggc acgtcaccaa cgaaactata acaatgatgg agaagtcaag
      301 ttccttcaac gctttaatca agaggtagaa acccacggca tgttacatgt attgcgcaat
      361 gggatcaaag accgcggtgt gcgctttaaa gtagcttact tcaaacctgc ttcagagctg
      421 aatgatgaaa cgatggaaaa gtatgaagca aatagtttta cctgtactcg ccagtttgct
      481 tacagtccta ataaccataa aaccattgat atggttttgt cgttaaatgg tattccgctc
      541 gttgctttag aattgaaaaa ccagtacact ggtcaaacag tagaaaatgc caaactccag
      601 tttcaaaaag atcgtgatcc aaaagaattg gtatttcgtt ttaatcgccg cttcttagtc
      661 tattttgcgg tggaccatac agatgtttat atgacgacaa agttggacgg taaaaaaacg
      721 tacttcttgc cattcaatca aggttccaat gggccgggaa gagttggtgg agcaggcaat
      781 ccgtccaata aagatggcta cccaactgcc tacttgtggg aaaaggtgct tactagagac
      841 aatttgatgg atattatcca acgttttatg aatctagaga ttaaaaaaga aaagcaaggc
      901 gacagagaag tcacaaagaa aacgttgatt tttcctcgtt atcatcagtt agatgtggtt
      961 cgaaaattgg ttgcagatgt aaaaagccat ggtagtggag aaaattattt gatccagcat
     1021 agtgctggtt ctggaaaatc taatagtatt gcatggctgg cataccattt agcctctatt
     1081 catgatcaac tgaactaccc aattttttca tcaattatta ttgtgactga tcgaacagtg
     1141 ttggatcgcc agttgcaaaa tactatcatg aactttgatc atcagacagg tttggtagaa
     1201 acgattgacg atcgcaaatc ctctcaagat ttaaaagaag ccattaatga tgggcgtaag
     1261 atcattatta ccactttgca aaagttccca gtgatctatc aggaagtaga tcaagtggaa
     1321 ggtcgtaatt ttgctgtgat tgtggatgag gcacattctt cacaaactgg gaacagtgcc
     1381 cagaagctaa aagccgctct ttcggacaaa tctaaatcct tggaagagta ttatgaactg
     1441 gaaggcgaag ttgacgatgt attaccagat gctgaagatg aactagttca acaattggta
     1501 gcacaaggta aacatcaaaa tttaagtttc tttgctttta ccgctacacc gaaagaaaag
     1561 acattggaaa tgtttggtac gaaacaacca gatgggcatt tcgaaccctt tcatgtttac
     1621 tcgatgcgcc aggcgattga ggaaggcttt atcttagatg tgctgaagaa ctatatgacc
     1681 tatagtactt cctacaaaat tgcccgtgaa attgaagata acccagaatt accaaagagt
     1741 gaagcaaccc gagcgattgt tcgtttccaa tcattgcatc cttgggcagt tactcaaaag
     1801 acggctgtga tggttgagca attccgagcg gttactcgta cagctattgg tgggcaggca
     1861 aaagcaatgg tcgttaccgc atcacgctta cacgctgttc gctatatgaa agaatttaaa
     1921 aaatatatag cgaacaaagg ttataatgac ttggatgtgt tggttgcttt ttctggttcg
     1981 gtagtagatg gagagactga atatacagaa acgaagatga atgtcacaaa atctggtgag
     2041 aatgttcggg agaatcaatt aaaggaagct ttccatacag ataattttaa tatcttgatt
     2101 gttgctgaaa aatatcaaac gggttttgat gaaccgttgc tgcataccat gtttgtcgat
     2161 aagaagttaa agggtgttaa agctgttcag actttatcac gactcaatcg aacaatgcct
     2221 gggaagaagg atacctttat tcttgatttt gtaaatgatg ttgaagatat tgaagctgct
     2281 ttccaaccct tttatgaaca aacaaatttg agagaagaaa tcaatgttaa cttgatttat
     2341 gatactcaaa gcaagctacg aaaatttaat gtttacaatc aaggtgatat tgaacgtgtc
     2401 atgaaattgg tgaaacaagc ccaaaagcaa cagaatgagc gcttgcttgg acgaatttct
     2461 agcatgttta agccgattat ggatcgctat gaggaaatga acaaggataa tcagtaccag
     2521 tttcgggtga ctctacgtaa ctttggcaag tggtatgatt atatttcaca acttgatcgg
     2581 ttattcgata tggaactatt ggaggaacga atttttactg gatacttgct gaagtttcta
     2641 cctaaagaaa cacgagaaaa tattgatatt tctgacaaag taaagttgga atattataag
     2701 ttacgtcagg acttttccgg tgaaatccat ctagtgaata aagaaggcga gctagtaaat
     2761 ccaaagtcaa ttgatgcagg aatcaaaccg ccggatgaac gagattcact agaagaaatc
     2821 attcataaaa tcaatgaacg atttccaaat gattttgacg aaggagaccg ggtgttagta
     2881 gagattttgt atcggtcatt tgccgataac ccagatttga aggttgtcag catggctaag
     2941 aataacgatg ctcagatgtt tgaaaaatcg ctgttccctg atgtatttaa agataaggtg
     3001 atggagcagt acgagaagaa caatgcagct tttgagaaaa tgtttgggca tgatgataag
     3061 tactttgatc tagtatatac attagttgct agagatttat acaagttatt acggacgaaa
     3121 taaaatttat aaataaggat tagacaatac aatttctctc aaaaataaag gagaagttgt
     3181 attgtttgct tttaagaaag ccctgaaaaa tttgttacgt actaaattgt ctgaactctg
     3241 tcatatcagc tatgtcagaa taaaggaaat agatttattt ttataaacag tgtcgtctcg
     3301 gagatttaaa ctatgtcaac gacaattgaa gtgaacaaac aaagcgtgaa gcagtttctt
     3361 gaaacaggga aaatcaaaaa attcgttatt ccagaatatc aacgtcctta tgcctggact
     3421 gacgagcaaa accaagtact atttgatgat ttagcggagt acacagcaaa caataacgag
     3481 agtacttact ttttaggaag cattgtggct tatgaaaatg atcataacga gcaagaaatc
     3541 attgatggac agcaaaggat aactacgcta tttttatttt tgagagctat ctatgctaaa
     3601 ttagaaaata gttgtgaaaa ggaagcttta ttcttgaaaa gccaaattga acccgcgtta
     3661 tgggaacaag atgatttaac aggtgaggtt aaaccagata aaattctgat tatgtctcga
     3721 gtaatggggg atgaggggaa tgaagaattt gctagtattc tagtaagtgg tgaagcagat
     3781 gttaaatcaa aaagtaatta ttcgaagaac tatattttaa ttcaacatct attaaatgag
     3841 tatgcaacaa acgaaccact atcattttat cgttttatca gta
//
LOCUS       DACKAC010000123         3682 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.128, whole genome shotgun sequence.
ACCESSION   DACKAC010000123 DACKAC010000000
VERSION     DACKAC010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3682
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.128"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..278
                     /locus_tag="I0B46_002683"
     CDS             <1..278
                     /locus_tag="I0B46_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HAR2084923.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            421..642
                     /locus_tag="I0B46_002684"
     CDS             421..642
                     /locus_tag="I0B46_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAR2084924.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            658..1500
                     /locus_tag="I0B46_002685"
     CDS             658..1500
                     /locus_tag="I0B46_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084925.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1563..3365
                     /locus_tag="I0B46_002686"
     CDS             1563..3365
                     /locus_tag="I0B46_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HAR2084926.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYCWKYKD"
     gene            complement(3424..>3682)
                     /locus_tag="I0B46_002687"
     CDS             complement(3424..>3682)
                     /locus_tag="I0B46_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAR2084927.1"
                     /translation="GITISIPPRKNMDKSEKLDHSLLGKQRKTVGTVFSLLEKLGCQN
                     FNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         1313 a          523 c          749 g         1097 t
ORIGIN      
        1 aagaactcat caagctggct aataagggtc aagattatga acatctagca gatgagattg
       61 atgagctgag agacaagcga cagctccttt tagtagaaga tgcctccctc agtggcgaga
      121 acgagcgaat caatgagctg attgaattta tccgcaagaa caaattccgt accttagagt
      181 acgatgataa gcttgtaagg aagataatcc agagcgttac agtctatgaa gaacacttcg
      241 tcatatcctt caaatctggc atcgaaatgg aaatatgaaa accagaagta aatagcccat
      301 gattctacag tagagtcgtg ggttttcttt tgcttgctga ttataataat attttaataa
      361 ctattgttat tataaaccaa atgatttata atattcttag cgtaaacttg gaggtgctgt
      421 atgactacgg cagaaatgat taaagaactg tgtgagcaaa tgaatataag tgtttccgaa
      481 cttgctagac gtattggaca gactccacag aatttcaata agaaattaca acgagaaacg
      541 gtaaccttag atgagttgaa agcgattgct gatgtgctag gtgtcaagtt tgtgcaggct
      601 tttatattgc ctgatggcaa tgaaataaag ataggtaatt aggaggtgta atttgtattg
      661 aaaatcataa caataaatga tgttgagtat gctgtatttg ctgctaatga aggtacttcc
      721 aaaccacaac ctcatatcat agaaacgaaa tctggtacta ttccagaagg aaaacaactg
      781 agtttattaa aagaatatct taagcagaat gatatttccc caataaaagg agcaacaaca
      841 tattggtgca ttgacaaggt tcttaagctt gattcaagta aagaaaaaac tataagtgaa
      901 acaatccata aacaaaaata cttatcttta actgaagaaa atattgagaa acagcataag
      961 tttgtaggtg caagctctaa ttatgggaaa gaaggtctaa taatacatga tgttttgaat
     1021 gcatttcctc ttcataatga tttaaatact attgcgatga agattgcggt aattgatgta
     1081 acaaattcaa cacatctttc tcaatataag agcaggttgt ctctatatga tttggctaaa
     1141 gtaattctgg agattccaaa ttttgatgat cggttggcaa aaggagatcc tcagttgata
     1201 aatattatcg caaggaacat tggtgcagtt aatatgttct catttgcatc caagtactgt
     1261 acatatcata atgtggaagt gtgtgggaga gatgattact ccatctttga tggtatcgtt
     1321 aaaaatactc ttccacacta tatccaagga ctaactacga ataagattga tacgtggcga
     1381 agaagttttg attacgaggc atttaacgaa tgtgttggaa aactacttga tgaaaataat
     1441 atacatattc cttttagaag aagaaagtta gatcacttct tgtggtatgc gaataggtag
     1501 aatataagaa aaaggcatcg taaaagtgat tctggataaa attaatgggg gaggaaataa
     1561 tagtgggatt atttgatttt ctaaagaaaa aacaaggaaa ggttagtaaa cacgttgatt
     1621 atcaactggc ttctagtcta tttcgtactg gtaaagtttt tgctgaaaaa ttggaagtgg
     1681 acatatcaaa tgtagatgag tttcgtaaaa cttttatcgc ttttgatgtg gaaacaactg
     1741 gcctaaattc ttcttcagat agaattgtag agattggtgc ggtgctattt aaagaaggag
     1801 ttccaattca atcattcgga actcttgtca atccaaaagt tagaattgca gcctcagctt
     1861 cggcaataaa taatataact aatgaaatgc taaactctgc tccaaatgag caggaagttt
     1921 acccccagtt ggtagaattt ttaggagatg cactagatgg aaaaatcact atgtgtgcac
     1981 acaatgcaag tttcgatttt ggatttttaa gtaacacatt ttctagactt gggtatgatg
     2041 ctaaaattaa gtatgttgat actctgagtc tctctagaaa acatataaaa ggcattgtaa
     2101 atcataagca agagactgta gcggaatatt tcggattaat aaatgacact gcccatagag
     2161 cggaatcaga tgcagatata tgcggaaaga ttttttgggg tgttttagat attattgagg
     2221 agtcgttgga agaacaaaaa aagcaaatag aaaagatgac tccagacata aaagaacttg
     2281 aagcttgtgc ttttattcaa gatattattg agaaaaaagg atgcgacacc agttggatca
     2341 ggtatcgaaa aaacagtaat aattatgtcg atgtaacttg tctatataca ttcttaaagt
     2401 ttaagtttgc taagaaggga aattacatca ttattagaaa agatgctaca aatggaattg
     2461 atttacctat agaaccttgt actgtaagtg aaggcggtac aacaaatgtg cgagtctatt
     2521 ttcgtagccc ctttgactta acgcctcttt caaattatat tttcagtgct tatagcgatt
     2581 gttataattc tatgaatgaa tatatgagtg ttagtaatta cactaaaaaa gaagcagagc
     2641 aaagccttag tatgctgaaa tctatttcaa aatcagcgat gaaagagtta ttattcgatg
     2701 caaaaaatcg tggatatgat gagactgaag ttaacctaaa tattgaaaaa gtgatatcta
     2761 gagaagatgt aattattaac gggttgcata atcgagtttc attaaacaag atacgaaata
     2821 tagggaattg ggagaaagga tttgatgcag gttatccata ttgggaacgt ggagaaactg
     2881 ctaggaaaaa tggcaaagta gatgaagcta ttgcactttt tgataaagct cgatataacg
     2941 gctatgatgc tcctgcgcta tatgattctt atgctaaagc ttatcgaaaa attaaggatt
     3001 acgataatga aattctcatt cttgatgaag gaattatgcg taaaacacgg cacgatgttg
     3061 gtactttaga ggctcgtagg gataaggcaa ttaagctatt atttgctaaa caagaggcag
     3121 agcggatagc taaggaaaaa tctgattttc taaaagcgaa caaaaaagag gatgcgatta
     3181 gtgagcctag caagaagcgt ggaagacaga ttattcaaat gactgatgat ggaacaataa
     3241 taaaagaatt cgatactata gcttctgctg taaaagaagt tggcgttaac tcaaaaagta
     3301 ttagggatgc tgcaaatgga gttcaaaaac atgctggagg atactgctgg aaatacaaag
     3361 attagcagat aatctaagat aacctaggat cttcttaata gatacccgtg gtgctagtta
     3421 attttaatat cctaaagaat atctcaaagt tccttcaaaa cgtcgttgtg ctcgacttaa
     3481 tagaacactg taagccagta atatgctttc aaatctactc tctagccctt taacagaacg
     3541 tgagttgaaa ttttgacaac ctaatttttc taacaaagaa aaaacagtcc caactgtttt
     3601 tctttgtttt ccaagtaagc tgtggtctag tttttctgat ttatccatgt ttttacgtgg
     3661 cggaatggat atagtaatgc cc
//
LOCUS       DACKAC010000124         3546 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.129, whole genome shotgun sequence.
ACCESSION   DACKAC010000124 DACKAC010000000
VERSION     DACKAC010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.129"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..391
                     /locus_tag="I0B46_002688"
     CDS             <1..391
                     /locus_tag="I0B46_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAR2084928.1"
                     /translation="RKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSYHVRNIDLMQS
                     TGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCATVDEDLEYQIF
                     NTELFEEYTYEVTGNIYENSELLEEQR"
     gene            388..945
                     /locus_tag="I0B46_002689"
     CDS             388..945
                     /locus_tag="I0B46_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAR2084929.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            942..1238
                     /locus_tag="I0B46_002690"
     CDS             942..1238
                     /locus_tag="I0B46_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084930.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            1315..1728
                     /locus_tag="I0B46_002691"
     CDS             1315..1728
                     /locus_tag="I0B46_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAR2084931.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            complement(2186..2461)
                     /locus_tag="I0B46_002692"
     CDS             complement(2186..2461)
                     /locus_tag="I0B46_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084932.1"
                     /translation="MYKPYMISYDLNNPGQKYDKVFEIIKEFGAYIKLQKSFWLVKTN
                     LNPNQMCEKLNTVLDNNDSLFICELQKNYQGRATEENWKFINEHIFS"
     gene            2915..3121
                     /locus_tag="I0B46_002693"
     CDS             2915..3121
                     /locus_tag="I0B46_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084933.1"
                     /translation="MLGQCFGNHARFDSLPATLNAYDGYDYRKKIVKKLYGATYGNVV
                     SVLSVHHQASVTVAEVEDAAVNGE"
     gene            3317..3484
                     /locus_tag="I0B46_002694"
     CDS             3317..3484
                     /locus_tag="I0B46_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAR2084934.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
BASE COUNT         1221 a          558 c          753 g         1014 t
ORIGIN      
        1 acgaaagaac gtaatgagag atgtagccgt cttgcatttt actaaaaacg gcaaaacaaa
       61 ctttattgaa tattggataa atcctaccga attgaaatca tatcacgtgc gaaacatcga
      121 cctcatgcaa tccacaggaa tgaaagataa gaatggtgtg gatatatttg aaggggatat
      181 agtattagtc agcgtgcgaa atggcttcga ttacttagat aataaagtct gtattgtcaa
      241 aaattcaata gattattccg gattagtttg tgccactgtt gatgaagact tagagtatca
      301 aatttttaac acagagctgt ttgaagaata cacgtacgaa gtcaccggaa atatatacga
      361 gaatagcgag ttattggagg aacagcgatg aataaacagg aagcaatcaa aaagttagaa
      421 agtattaaag cgataggaaa tgatgcaata gctgcttgct ataacgagag tataaattca
      481 ggtattacgt taatgaaaaa aatagacgaa ccgcagaaac cagttattcc acaactcgtg
      541 gccggttggc ttgagaaatc tacggaccct tttacaaaag ctgaaaaaat agcgtattta
      601 atcaaatcta aagatggtga ttcatattat ttctgtgatt ggtttgtacg agatggcata
      661 gtgacgcaag agcaaggaga agaattactc gcttgggcaa caagacaatc atatgaaaca
      721 ctattgagcc tatacaacgg ctacgaggtt gagaaagagc cgttatgggc aataaagaat
      781 gccgatggaa actatcttac taaatgtgct ttatggggaa aagatggagt aaattatagt
      841 tttgaatgca atccatctca tcgattgctt ttcactgata aagcaacagc ggatgctgca
      901 gcattgttgg tgaatggaac agtggaagag gtggtagaaa gatgaaacta aaagacggat
      961 tttacgctag cagtcacggt atcggcggtt taatgctaga tatgccgaca aagaacccta
     1021 aaacacgcaa gaaatcaaaa ttcaaagtcg gtgacatggt ccgctgtgaa gcagaagagt
     1081 tcgtttatcc gttcagagga tacgttaaaa agatactgtc aaactcagca atcattcgta
     1141 ttgaaaatac aatgaagtgt gatcgttcga ctgcaaaaca caagcattat ttagctgtag
     1201 cgagattagt tgacatggaa gtaatcaaga gcaaataaaa aaagccggat cgctccgact
     1261 gattcaataa atccaacaca tttattatat cacataaagg agcggtttga cttgatgcaa
     1321 ttgttacgag aggtagattt caaacagaca agatgtaatg cgagagatgt gctgaagaac
     1381 tttcggcgtt tggagcggat ggcaggtcgc tctttgatag atattaagtc gccgattata
     1441 acggatatgc cgaaggcacc gaagcatggc aataaggcag aagacgcgat tattcagatg
     1501 atggatatag aaacggagag agatgcgatt ctagcggctt tgatggctct tagtctgatt
     1561 agtcgtcaga tactctacta cagcttctgt gtgccagata gcttctcaaa ctacagaatt
     1621 agccgtgaag tgggttattc agaaagaagt atacaacgga tgaagtcgga agctctaata
     1681 gagtttgcag aagcatataa acacggaaaa ataattgctt ataaataatt tggcggtttt
     1741 ttggcggaat gatggcggtt tttagacatt taccagtgtt attatggtag tgtcgaaaga
     1801 ttaggaaaca ggatcgacaa aataaacgaa gggaggcaat ctccctcatc gttttaaact
     1861 tcttcttaga tagacagcaa ctaattatga agaaaaggat gtgaattcaa ctcctcctaa
     1921 attcttcatc ggttgctgtt tattttgatt agacagcagc actcacaaaa ctaaacatgt
     1981 tcttgtcgtc tttcaactgc tgctgtctat taatttatgt attggaggga agaaaaatga
     2041 aattatcagt agaaggcact ccagaagaaa tacaagaatt gctccaagct attggtagta
     2101 gcaaggagca aaaggtaagt gtttctaatt cagatattga cggtatttgc aattgtttaa
     2161 acagagaaaa aatatctaat ccttactaag agaaaatatg ttcgttaatg aatttccaat
     2221 tttcctctgt agctctacct tgataatttt tttgcagttc acagatgaat aaggaatcgt
     2281 tattatcaag tactgtattt aatttttcac acatttgatt tggattcaaa ttagttttaa
     2341 ctaaccaaaa agatttttgt agctttatat atgccccaaa ttcttttatt atttcaaata
     2401 ctttatcata cttttgccct gggttattaa gatcataact aatcatgtag ggtttataca
     2461 tgttgtcttg cacatccttg ttttatattt cagcggacca ctcgctgata aataaaatta
     2521 tacgcttagt atttattttc acaatattaa tttatcgtaa taacttttga gtaaatagaa
     2581 caaaaaacct gcactagttt ccgctagtac aggcagtgac tatatcggtt gataatctag
     2641 catatattaa aaataattgc aagaaaagaa atttatagtg gtttatacca aattatcaaa
     2701 gcaatgttat tttgttgctg tctattgttt tttaattatt cacacgataa ctaaaggtgg
     2761 gtgaagagaa atgattccat taataatttc aatttttgcg ctctgtctta atgtctatat
     2821 gattggattt aaaaatgggc aaaataaaaa atagtagcag ccaagaataa ttttatagtg
     2881 tcactgtggc ggaaagggta gacgctaagc atgtgtgcta ggtcaatgct tcggcaacca
     2941 tgcaaggttc gattccttgc cagcgacatt aaatgcctat gacggttacg actaccgaaa
     3001 aaagatcgtt aagaagctat acggtgctac gtacggcaat gtagtaagtg tgctatctgt
     3061 acaccaccaa gcttcggtca ctgtggcgga agtagaagac gcagcggtaa atggcgagta
     3121 gcctcgtgag agcctggtaa gttctcgtga gtggtgcaat cccactccag cgactttaag
     3181 caaccgaggc atcggcggtt taaaaatata ggggtgcgca atttcgtacg cgttttgtgc
     3241 atcgtgcaag ttactattac atattagatc actctttgag tggtcttttt atttttgcac
     3301 aaaggaggaa acaacaatgt atagaccaca atacttagaa cagaagtatg aagtaatcac
     3361 tgttcataaa ggtaatggcg aaaaagtata tgagtataga agaccaataa agagcgatac
     3421 atataaacga aaggaaaaca atgaagttat tccattgtat ggcaaaagaa tagctaagca
     3481 ttaaataaga ttgcgaaagg agacggaaca tgaccgagga attctataga tggctattac
     3541 agttga
//
LOCUS       DACKAC010000125         3392 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.130, whole genome shotgun sequence.
ACCESSION   DACKAC010000125 DACKAC010000000
VERSION     DACKAC010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.130"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(67..2559)
                     /locus_tag="I0B46_002695"
     CDS             complement(67..2559)
                     /locus_tag="I0B46_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAR2084935.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(2566..3033)
                     /locus_tag="I0B46_002696"
     CDS             complement(2566..3033)
                     /locus_tag="I0B46_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAR2084936.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(3169..3242)
                     /locus_tag="I0B46_002697"
     tRNA            complement(3169..3242)
                     /locus_tag="I0B46_002697"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3206..3208),aa:Arg,seq:acg)
     gene            complement(3300..3373)
                     /locus_tag="I0B46_002698"
     tRNA            complement(3300..3373)
                     /locus_tag="I0B46_002698"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3337..3339),aa:Pro,seq:tgg)
BASE COUNT          927 a          750 c          557 g         1158 t
ORIGIN      
        1 aaatcttgtc acaacctctt aatcaagtct tactattata aaaattcagc tacacaggcc
       61 aagtaactat agggaaccta ctgtttcctt tttaggtccg cgaacattca atgtgatctt
      121 accttttgaa gcacctactg ttacgtggtc acctaaacga atttctccgc caagcaaagc
      181 ctcgctcaat cgatcttcaa tttctttttg aagtgcgcga cggattggac gtgcaccata
      241 ttcaggatca aatccagctt tcccgatcac atcgattgca gatggtgtga ttttcacttg
      301 aatatcttgt tctttcaatc gtttgataat cgctttgctc atgatttgga cgattgtatg
      361 gatttcatct ttactcaatg aatggaagac gacagtctca tcgattcgat tcaagaattc
      421 aggtcggaaa gctttcttta actcttctaa gatacgtttt tgcatggctt tgtgatcttt
      481 agtaacatct tggacattga atccgacatt tttttcttct cgaatttgtg tcgctccgat
      541 atttgaagtc ataatcatga ttgtatttct aaagtccact tttctacctt tagaatctgt
      601 taaatgacca tcatccaata cttgcagaag taaattgaaa acgtctggat gtgctttctc
      661 cacttcatct aagagaatca ctgaatatgg tttttgacga attttttctg tcaactgtcc
      721 accttcatca taccctacgt atcctggagg agacccgatt aatcggcttg tactgtattt
      781 ttccataaat tctgacatat caacacggat cagggcatct tcacttccaa acatgacttc
      841 tgaaagtgct ttggccaatt ctgtttttcc gacaccagtt ggtccaagga acatgaatga
      901 accaattgga cgatcaggat ctttcaatcc gcttcgtgca cggcggatcg cacgtgaaac
      961 tgctttgaca gcctcatctt gaccaacgac acgttcatgc agaagtcctt ccagttctaa
     1021 caagcgttcg ctttcttttt tctccaactg ctgtagaggg acaccagtcc actccgatac
     1081 aactgtagca acatcttctt ctgttacacg atcagaataa ccttgctcct gttttgcttc
     1141 aattgcagaa actttttgca gctttttacg tagtgctttt tcttgtctgc gtaattgagc
     1201 agcattttca aagtcttgtt tttggatcgc tgcttccttt tcttgtacta gttcatcgat
     1261 ttctagtttg atcacagttg attttgttaa gtgatcggtt tgatccaaac ggactttcgc
     1321 tgctgattca tcgatcaaat caattgcttt atcgggtagt tgacgagaag tgatataacg
     1381 aacagaaaga ttgactgctg ctcgtacagc ttcatctgtg atctccactc catggtgctc
     1441 ttcatatctt gtacgtaatc cttgaagaat cacttctgct tcttcaggag ttggctcatc
     1501 tacttgaatg cgagcaaaac gtcgctctaa tgcagaatct ttttcgatat atttttgata
     1561 ttcatctagg gtcgttgctc cgatcgtttg taattctcct cgagctaacg ccggtttcaa
     1621 gatattcgaa gcatcaattg ctccttctgc tccacctgca ccaattaagg tatgcaattc
     1681 atcaatgaat aagattactt gaccatcatt atagatttcg tcgatgacct ttttcattcg
     1741 atcttcaaat tcaccgcgat atttcgtacc agcaaccaaa gatcccatat caagcatcat
     1801 caggcgcttt tcttgcatgt cttctggcac ttcacctaaa atgatttttt gagctaaccc
     1861 ttcagcaatg gctgttttgc ctacaccagg ttcacccacc agtacagggt tattctttgt
     1921 ccgtcggcta agaatctgga taagccgttt cacttctctc gaacgaccaa caactgggtc
     1981 cagcttgttt tcttttgcta atttcgtcaa atcacgtgct aatgaatcta gcgtaggtgt
     2041 tccttgcggt gtttgccgtt tatcttgtac atttacattt ctacgacgat tcattccttt
     2101 tccagcagaa tcaccagcta gccccatttt ttgtttcaga acttgacggg cttttgaaag
     2161 gctgatacct aagttgatca tgattcgagt agccatgata ctttcatctt ttaataagct
     2221 catcaaaata tgttctgttc caactttttg agaaccagtt ttcttcactt cgctgctagc
     2281 taaagcaaaa acttgtttca ttcttggtga atatggaaga aagactcctt tagggtaaga
     2341 acggatacca ccataaccaa tcagatgttc aatttcttca taaatttccg tttcattcaa
     2401 atccatttca cgtaatactt ttccagcaat tccattttgc tcgaccacta aggctagtaa
     2461 aacatgttct gaactaactg attgatgttt caaccgtttt gcttcttctt gtgcaatttc
     2521 taatactgct tgggctcttt cagtaaaaag ttcttccatc taatctcact tcccatcttc
     2581 ataacttaaa cgttccagaa atgctcggag aatccgagca cgtgtatgat cttcatattc
     2641 attgaaattc aatgtgtttt ttgcaattgc actcaacatc aagtttcctt ctttttttgt
     2701 aattatttgg tcttcataaa gtttttgaat aatagcaaaa gcatttttct cactgattgt
     2761 atcatcaaat aactgattta tctgctcaag taattgtttt ttatctgaaa tccttacttt
     2821 tgcaattcgt atataaccac caccaccacg tttgctttct actagataac cacgttggat
     2881 ggtaaaacgg gtattaatca catagttgat ttgtgaagga acacagttaa agaggtctgc
     2941 catttcaatc cttcgaatct cgatcatttc actattttcc agaattttct ttatatatga
     3001 ttcaattaaa tccgaggtat tctgatgtgc catcttattc attccctttg actaatattg
     3061 accttaatta taacgaattt gataatagaa agaaagaaga acgctttttg ttaaataaat
     3121 cctttagaat caacaaaaaa aaatacggaa tatactattc cgtatcgacg cgcccaagag
     3181 gagtcgaacc cctaaccttt tgatccgtag tcaaacactc tatccagttg agctatgggc
     3241 gcatattcaa tttaaaaaaa ttataaatca tttgaaaaca aaaagcaaga taaaaaaaat
     3301 cgggaagaca ggattcgaac ctgcgacccc ttggtcccaa accaagtgct ctaccaagct
     3361 gagctacttc ccgtttaaaa aataaaaaag ac
//
LOCUS       DACKAC010000126         3382 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.131, whole genome shotgun sequence.
ACCESSION   DACKAC010000126 DACKAC010000000
VERSION     DACKAC010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.131"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..246
                     /locus_tag="I0B46_002699"
     CDS             <1..246
                     /locus_tag="I0B46_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084937.1"
                     /translation="EKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDNCCSKYKVNQVRE
                     ALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            349..663
                     /locus_tag="I0B46_002700"
     CDS             349..663
                     /locus_tag="I0B46_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAR2084938.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            641..2335
                     /locus_tag="I0B46_002701"
     CDS             641..2335
                     /locus_tag="I0B46_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAR2084939.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            2355..>3382
                     /locus_tag="I0B46_002702"
     CDS             2355..>3382
                     /locus_tag="I0B46_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAR2084940.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGV"
BASE COUNT         1212 a          467 c          686 g         1017 t
ORIGIN      
        1 gaaaagattg aagagtattc atcttatact cagggattaa ttaaacgttt gatatacgtt
       61 cgctatgtag gtattaggga tctgttatca gataactgtt gtagtaaata caaagtgaat
      121 caagtaagag aagcgttgaa taaagataat aacgtcgaaa gaataaaaaa tgtttttgga
      181 tatagtattg aagagattaa ttattacatt gacttcgctg aagctttcat tccgatggtg
      241 agataacccc cccttaaaat aaatcgcaaa ttttttgggg gtgatgaaac ggagggggct
      301 gtcaggaaaa gagatttttt cgaactttat catgaaagga gggctaaaat gtttaaaaac
      361 gaattgtctc aaaatcgcta cagagaaaaa ttacgccgct ctttaataag ccaattggaa
      421 agtcagaaaa caaatattga gccattctta gataatgttg atcgttatat cagtttatgg
      481 gaaacggcga tatcactgga agaagatata tccgagaacg gcattagact ggagaatggt
      541 aaaaagaatg aatcagtagc gttgcttgtt tctgtcaaca aacaaatggg attgatgttg
      601 gataaacttg ccattactcc tgaattggta ggtgaagcaa atgaatcaat tcctgagtta
      661 taagcatatt gaaaattggt tcaaagctat agaagaaggc actatcaagg tatgcaaaga
      721 gcaattattg ctaaaaaatt atctagaaga aagagtcttt actagagaag atatttactt
      781 cgataagcag atggtagagg attcaatcaa tataccagca caatactttc cattcgaatt
      841 aattccgtgg gaaaaatttc tacaatgttt tatttatggt gttcgatgga aaaaagataa
      901 aacactagtg ttcaatagat atctttcatt aatgggacgt ggtaatggta aaactggttt
      961 tgcttcttgg aacaacttct ttctactaac cgctaaacac ggtattaaaa attatgatat
     1021 tgatatctat gccaataatg aaagccaagc aaagactagt tttgatgatg tatttaaagt
     1081 aattaaagat catcctgatt tagataaaaa agtatttaaa gctacgaagg aagttattca
     1141 aaatatcgct acaaacagca aacttcgtta taacacggca aatgctagaa caaaagatgg
     1201 gaagcgacca ggtgcaaacc gctttgatga aattcacgaa aatgaagatt attcaatgat
     1261 aaatgtggct acttctggtg gtggtaaaat tcgagattat agagaatttt atgatacaac
     1321 taatggtcat gttcgtggtg gtccgcttga tgacattata gaagaatcaa aaatgattct
     1381 ttctggagaa cttggaattg acaaggatgg agcagaattt tctagtttgt ttccatttat
     1441 ttgtcgcttg gataacgata atgaagttga tgatcccgac atgtgggaaa aagcttgtcc
     1501 aactattaat tacaatgcag atttaaaacg gaaaatgttt caagaatact ctcaaatgca
     1561 acgtaatgct ggtttaagac ttacgttcat gaccaaacga atgaacagac ctatggaaga
     1621 tacacgattt gctgttgctt catatgatga tgttctgcat acgaaagaaa aagaatttcc
     1681 tgaaaaaatg gatgaagtga taggaacagt cgattttgct gatagacgag attttgccag
     1741 cgttgggttg ctaggaaaat acgataaaga tgtttatttt acacaacata cttttatcca
     1801 cgaatcagcc cttcgattac aaaacatcaa acgagaggtt atagatattt ctatagatca
     1861 aggaaaatca cagatcgttc atggaaaaaa tatagaagct gattatattg taggttggtt
     1921 tcttgaaatg agtaataaat attatattaa aaaaatcgct atggatatgt accgtgcaaa
     1981 aatattgaag cccgctttag aagaagcagg ttttactgtg gaaattgttc gaagcggatc
     2041 tgttacacat ggtatgttaa aagatctggt tgatgacctt tttattaatc aacgtttatt
     2101 ttttggtgac gatgcgatta tgcgttggta ttgcatgaat gtatatgaag agcatatttc
     2161 taatggaaat atacgctatg aaaaaataga acctgaaact agaaaaacgg atggcttttt
     2221 ttcattcctt catggtttga attttttaga tgatatttat gattctgctc ctgtaacagt
     2281 cacaaatagc tcagtagaaa atacaggaac tggatttact cctctagtat tctaacttga
     2341 aaggaggtga gaaagtgggg atttttcaaa aggcggtagg atacttcaca aaaaaagcaa
     2401 cggttccttt agaagaatac ttttgtaaat tgcaagttga ttttgtgtat cgaaaatttg
     2461 cgattgaaac ttgtattgat ttgattgcaa atgcgatgag taaagcggaa ttcaagtcat
     2521 atgaagatgg aaaaaataaa aagaatgatc tttactatag gctgaatgta gctcctaata
     2581 agaaaaataa tgcaacagaa tttagaaaaa aactgatcag gagattaata ttctacaatg
     2641 aagtattgat cgtttctccg tctaataatt ctagcgaaat atttattgcg gatagttggg
     2701 atgtcacaga atatgcattg aaagatgatg tgttttctca agtgcaaatt aacaacatag
     2761 tccttgatag agaatttcta gaaagtgatg ttatctatat aaaatacgca gatcaacaaa
     2821 ttaggcaact agtcgatgcg tattatcaag cgtatgggaa actcatttct agtgccatga
     2881 atgtttacaa gcgctctaac gctcgtagat acgtactgaa agggaattta ttccgaccgc
     2941 aagacaatac aacacaagat caaatcaata aaatgatgac atcacaattt aaggctttta
     3001 tggaagctga taatgcaggt gcggtatttc aattacaaaa tgagtacaca ttagaagatt
     3061 tcagcggaaa cttccaaagc aattcaagag atataaaaaa cttaatagac gacatctttg
     3121 agatgacagc agcagcgttt cacgttccga aaaacctact aaagggagac atgagtgggt
     3181 tatcggatca agtggacgct tttttaatgt tcgaaatcat accgattgct gaacttattc
     3241 aggatgcgtt taacgctagt ctctatgaag tagaagaata cttgtcaggg aattttgtac
     3301 gtgtggatac aactatgatc aagattacta gcttcaaaga tttggttgac gctattgatg
     3361 taggcattag aaatggagta tt
//
LOCUS       DACKAC010000127         3374 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.132, whole genome shotgun sequence.
ACCESSION   DACKAC010000127 DACKAC010000000
VERSION     DACKAC010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.132"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(162..2159)
                     /gene="tkt"
                     /locus_tag="I0B46_002703"
     CDS             complement(162..2159)
                     /gene="tkt"
                     /locus_tag="I0B46_002703"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAR2084941.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2261..2512)
                     /locus_tag="I0B46_002704"
     CDS             complement(2261..2512)
                     /locus_tag="I0B46_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAR2084942.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2659..3282
                     /gene="lexA"
                     /locus_tag="I0B46_002705"
     CDS             2659..3282
                     /gene="lexA"
                     /locus_tag="I0B46_002705"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAR2084943.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
BASE COUNT          920 a          740 c          626 g         1088 t
ORIGIN      
        1 ctcaagaaac acaaactaat ccgaagaatt ctttgttttg tcatttaaat gtaacatagc
       61 cgaaaacgaa aagtaatatt gatttattct tctaacacat aaagaacagc tgtctccaac
      121 ttgcagacag ctgttcttat cagtcaaaca attgcttgga tctatgatag ttgattgtac
      181 gtattcacga cattttctac tgtaaatcca aattcttcta aaattttagt gccaggggca
      241 gatgcaccga aatgatcgat tgtaatcgtt gcgccctctg ttcctacgta gcgttcccat
      301 ccaaatgatg cagcagcttc gatagccaca cgtttcttca ctgatttagg aagtacactt
      361 tctttatatt cagaagattg tttttcaaag aggtcaaagc ttggcataga aacaacagaa
      421 acatctttgc ctttttcagc caattctttt tgagctttca cagccaaatc aacttcagaa
      481 cctgttgcaa tcaaaatacc ttctggtgtt tctccttgtg atggagaaag aacatacgcg
      541 ccttttttca ccatttcatc tgctacttct tttgtagaag gcaatactgg aaggttttga
      601 cgactcaata caaggatagt aggcgcatct gttgtctcca tcgctacttt ccaagcagca
      661 cgtgtttcat ttccgtctgc cggacgaatc acttgaacac caggcatgca gcgaacagat
      721 gctagttgtt caatcggttc gtgcgttggt ccgtcttctc cgacagcaac cgagtcatgt
      781 gttaagacgt aaacaacagg agtatgttgg attgctgcta aacgaactgc tggtcttaga
      841 taatcgacaa agacaaagaa tgttccaccg tagatacgag ttccgccatg aagttggata
      901 ccattcatag ccgaagccat tgcaaattcg cggacaccga accagatgtt gcgtccttca
      961 taatgttcag gagtgaaatc tttatctgca gccaccattg tattattgga acctgacaag
     1021 tccgctgaac caccccagaa gcttggtata gctttcgata attcttggat gacttcttta
     1081 cttgaaacac ggcttgcttg actgcttcca acttcataag ttggcaattc tgcatcccag
     1141 ttttctggta gtttgccatc aaaagcatct tcaaattgtt gtgctaattc aggataagct
     1201 tttttataat tggcaaacat ttcattccat gcatcttcag ctttttgtcc ttcttctatc
     1261 atcgtttgat ggaagcgggc agctacttct tctggaacag tgaaatctgg atactcccaa
     1321 ccataaactt ctttagccat cttgatgcca tcttcaccaa gaggagcacc atgaacagca
     1381 gatgttcctt ctttaggagc accatagccg atgactgttt ttacttcgat caatgttggt
     1441 ttgtctgttt cagcttttgc ttcttcaatc gctttagaga ttgcttctag gtcatttccg
     1501 tctttcacta agatatgttg ccagccatat gcttcatagc gagcaccaac gttttctgtg
     1561 aaagcttttg aagttggacc gtctagtgaa atatcgtttg aatcgtataa aacgatcaat
     1621 ttccctagtt tcatatgacc tgccatcgaa cttgcttctt gagatacccc ttccatcaag
     1681 tctccgtcac cgcatattgc atacgtatag tgatccatca cattaaagtt ttctttgtta
     1741 tacgttgctg caaggtgcgc ttctgccatt gccataccga cagccattgc aatcccttgt
     1801 cctaatggcc ctgtagtggc ttctacacca tctgtatgat gtacttcagg atgtcctggt
     1861 gttttagaat cccattgacg gaattgtttc aaatcatcga ttgtcacttg atagccagct
     1921 aaatgaagca agctatacaa cattgctgac ccatgtcctg ccgaaagaac gaaacggtcg
     1981 cggtcagccc agttttttga tgtcgttgga tttactttca ggtgttttgt ccataacgca
     2041 tatgccatag gtgctgcgcc catcggcaat ccaggatgtc ctgagtttgc tttttggact
     2101 gcttcaatac ttaatgtacg aattgtgtta acgccaagtt ggtcaatttt gtcaaacaaa
     2161 ataacccctc ctattaattt aggttttttt tacctttatt tcttacactt tcattctata
     2221 agatgtcaca cgaaaaagca atctttttca gtatttttta ctaaaagttg tcatttcggt
     2281 tgtgtaagcc ttttttcttt tggatttctt ttaatttttc cggggtcacg tcattgccct
     2341 caggatcgac gactttcatt ccttcgatat gatgacgcat accggaacga aaagcttcca
     2401 aatactcttt gcgcaagttg atttgttctt gttcttcggc ttttgagaga ccattttcct
     2461 tctttttacg tgcaagttca ttgatccgct ctattttttc aggtgacagc atgctcagcc
     2521 ctccttttac tctccttcat attatataaa aactgaaaaa aaaacaacta gataaactaa
     2581 ataaaatacg aacattcgtt tgatttagcg ctttttatat ggtactattt atttatcaag
     2641 tgaaagaagg tgtaccttgt ggcccgacaa actgaatcaa gacaaatcga agtcttacga
     2701 tatatccatg aacaagtaac ccaaaaagga tacccgccga ctgtccgtga aatcggggaa
     2761 gctgttcagc tttcttctac ttctactgtt cacggtcatc ttgcacggct tgaaaaaaaa
     2821 gggtttatcc aaagagatcc gacgaaacct cgtgcaatcg agttgacaaa acaaggttta
     2881 gaaaagattg ggattcgtcc aacaactatt ccagtacttg gcgtcgtaac agctggagaa
     2941 cccattttag cagtagagga tgcttcagac ttctttccaa tccctcctaa tctttcttcc
     3001 gaagaaggca gtctctttat gctgacaatt cgtggagata gtatgatcaa tgctgggata
     3061 ttggatggtg acaacgtgat cgttcggaag caagaaactg ctcaaaatgg cgatattgtc
     3121 attgcaatga ctgccgagga cgaagcgact tgcaaacgat tctataaaga aaaaaacttt
     3181 ttccgtctgc agcctgaaaa cgacttgctc gaaccaatca tcttagatca agtatctata
     3241 ttaggacgag tagtaggact ttatcgtagt catatctatt aattgctact aattaaaaga
     3301 aaacttttta aaaagagttt gagacgttgg tctcaaactc ttttttcgcc cgggttcttc
     3361 ttctttcaaa tttt
//
LOCUS       DACKAC010000128         3373 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.133, whole genome shotgun sequence.
ACCESSION   DACKAC010000128 DACKAC010000000
VERSION     DACKAC010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.133"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            137..1105
                     /locus_tag="I0B46_002706"
     CDS             137..1105
                     /locus_tag="I0B46_002706"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAR2084944.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(1422..1946)
                     /locus_tag="I0B46_002707"
     CDS             complement(1422..1946)
                     /locus_tag="I0B46_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAR2084945.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            2233..>3373
                     /gene="scm"
                     /locus_tag="I0B46_002708"
     CDS             2233..>3373
                     /gene="scm"
                     /locus_tag="I0B46_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAR2084946.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDST"
BASE COUNT         1078 a          688 c          625 g          982 t
ORIGIN      
        1 aaaatgaacg cccaccctct tcttttttct ctttccttct ttcgtttttg ctttatgtat
       61 caaccgttgt ttcttcgaaa actattttgt tctctccttc ttcctgatag aatggatggg
      121 aaaggagctt gatgccatga caacgaaaga caaggtactt caattattga aaaagacacc
      181 agattttctt tccggagaaa agttggcaca gcaactggaa gtttcccgga caagtatttg
      241 gaaagccatc aaagaattgg aaaaggcagg ctatcgtttt gagcacgggc ctacagggta
      301 tcgttacctg ccctctgacg tactagatgc aaaagaaatc cagcaagagc tacggtcgtt
      361 tattcctgat ttgaaagtac aaacgatgtt gacttctgaa tctacgatga aagatgccaa
      421 actggcagca gctcaaggag attctgtttc gaagttattt gttgctgata cacaagaaca
      481 agctcgtgga cgttttggac ggcctttttt cgctgaacct ggacgaggaa tctacatgag
      541 tctcttgctc catccagaca aacggtttga tgaattgcct caatatacga tcatcgcagc
      601 cagtgcctgt gtgaaagcca ttgaatctgt aaccggtcgt tctgcttcga tcaaatgggt
      661 caatgatatc tacatggatg gaaaaaaagt ctgtgggatt ttatctgaag cagtcagtga
      721 tatggaaagc ggcaggatca gtcatatcat cattgggatc gggcttaatt tttctgttcc
      781 tcaaaatcat tttccagctg atctccaaga aaaagccatc tctgtctttc ccgacggaca
      841 agctagtgtc acaaggaatc agctaatcag caaaatctgg caagaattgt tcacttatct
      901 ttctcaccca gaaaaagcgt tggacttcta taaagaaaaa tcattcgtat tgggaaaaac
      961 cgtgtcgttc aatcaaaaag gaacgactta tgtcggtctt gcaaaagaaa tcggtgatca
     1021 tggcgaactg atcgtccaaa caaatgaaca gaagatgagt ttgtcttctg gcgaaatcag
     1081 tctcacatct atccgtacgc aataattatc agatttcaca aaaggacacc tgatctcata
     1141 cagtgtcctt ttttctgaat tctcttcaaa agactctcct tattctatca gctgacagtt
     1201 tctctatcct ctagaaaaag gtattctaaa aatatctata aagcatggta cactttcttt
     1261 aaatgaatca atgcaaaaaa caagagagtc atcagtttac acgatcaaaa gagatagcag
     1321 gccgcttcct ttttcaatct tatagataga ccatgggtaa cgataaaaac aaaaaagaac
     1381 ttgcgactta ttttgccgta agttcttttt tagctagtgc cttaatctct taatcccaac
     1441 aaacgtctcg catagatcac ctgaccagag tgcatagcag catcatcaat aatagaaacg
     1501 agacgcattc ctcgtgtgac tggtggtgtc cagttctcgt caattacttc atctagctcg
     1561 gaaggttcca gccttcttaa gtaagaaata gcaaaatcag cagctcctcg caagtatcct
     1621 agcagatctt ctatttgatt agcttggatc tgctttgctt gttccagact atgcttccaa
     1681 tcttgttcgt tatctgctat cgtcatcgga aaccggcttt cccagtcttg actatgccaa
     1741 attggagaag ttcctgctaa atcagcaatc tggaaatcta attctcgagc ggtatgccaa
     1801 gccagccaag tcattgattt gatctgcggg gctctatctt ccactggaaa acgattggcc
     1861 tcctcaatag agacgccttc aaacaaacgt tcaaaccgat cgtatgcccg atccacactc
     1921 tcgcatagca tatctaaata atccatttat tttcctcctt tttatttatc ctacttactt
     1981 tttcctaatt ctaggatacc tacttatatg ttactacaca cccttatttt ttacacttta
     2041 acagtcacca tcatcaatag tttggagatt gaaagaaaaa gatagtgtcg aactatcaat
     2101 tgaacaagaa gcaacaatat gaacgtttca ttaatattct ttaaataaac aatgattttt
     2161 caaacgaaca gtttaagact aggagaaaaa gagtataata ggaaaatgtt tgaaaggaaa
     2221 ggaaggggaa aaatgaaaaa aagggcaagt ttactagtcc tagttgcttt gttgttcaat
     2281 atcttttcgt atggtgtggc cgtttcagcg gaaacataca ttcctaaaga ccctgggaat
     2341 atttataatg atctaactgg taactatggc ttgttaggaa ttgctagtca attccacgta
     2401 tttgctaaaa ataagacaac tgtgaatgca catacagacg gaaatatagc aacacatgaa
     2461 cttgatgcaa aaaataattt tggtacagag cttcatgaag gtttattgaa ccaagaaata
     2521 aattatatac aaaaaactga ttctttcgct aattcttcag gcatacctac aggtagcaca
     2581 agaatgaata aatttgtcgt tggcagtaac gtggatgtag cacttcaaga tggtcatcct
     2641 acagtcaatg gatccaggct tgatcactta actccagatg atttttataa agatcgtgct
     2701 gggcatgaat atattgattt cgagcaggaa tttgctaaat tgaatgtagc agcaaatgat
     2761 cttgcaacga tcactccagc taaaacatat actgctgcag attttccaga catgaacaat
     2821 cggacaatcg atttaactga tttatcagat accgggtttt tattggtaaa tatcgatgca
     2881 gaagtgttaa cgatgaatac gccacttcaa atcattaatc caaatgatca agtagttgta
     2941 tttaatgtca tcaacagttc ttctgcattg aacgtacagt caccaatcaa atacaatgat
     3001 cgttcaaatc atgaaacaga agatttttcg gatgcgaata tctcttggaa ttttggaaat
     3061 gagatgactg atttgacaat cagtgctcct tttcaaggaa caatccttgc gcctaatgct
     3121 actatccgca ttactcaaaa ccaagacgga acgattatag gaacaaatgt gattttagat
     3181 gcagctacca atcgttggga tcctaacgaa atatttatca gcgacaatgg tacagatacg
     3241 acagacacaa gcgacagtac agatacatct accacaagtg atagctcaga ttcatcaact
     3301 tcaagcgaca gtacagatac tacctctacc acaagtgata gcacggatac atcagcttca
     3361 agcgacagta cag
//
LOCUS       DACKAC010000129         3292 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.134, whole genome shotgun sequence.
ACCESSION   DACKAC010000129 DACKAC010000000
VERSION     DACKAC010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.134"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            37..588
                     /locus_tag="I0B46_002709"
     CDS             37..588
                     /locus_tag="I0B46_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF334 domain-containing protein"
                     /protein_id="HAR2084947.1"
                     /translation="MKRLEASTSNFNNKLDVFEEKTKHARLNFETTAKHYIKRLDEDN
                     LKLDFQQAIQDELSDTKDEIREVTKQAREETKEYKEILESKIKDHNKVVDKSNTALKV
                     MTKGVTNIFFVLIIFVLVMLVTGPIGHFFGIEHLYSFINGFIDDHESAWRYLMLILYA
                     VPYVFFAIILWLIALFFRVFNDL"
     gene            1264..1530
                     /locus_tag="I0B46_002710"
     CDS             1264..1530
                     /locus_tag="I0B46_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084948.1"
                     /translation="MAKITKNEQENLVENYTKKPAYSNSRLDAHFVCTSNPKLSFDAM
                     TIVGNLNKNSAKKLSDFMSLDPQIRLWDILQTKFKAKALQDPLC"
     gene            1588..2100
                     /locus_tag="I0B46_002711"
     CDS             1588..2100
                     /locus_tag="I0B46_002711"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003321317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAR2084949.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLC
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            2097..3134
                     /locus_tag="I0B46_002712"
     CDS             2097..3134
                     /locus_tag="I0B46_002712"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAR2084950.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT         1213 a          555 c          653 g          871 t
ORIGIN      
        1 agcgagcaag acaggaacga gagaacaaac atactcatga aaagactaga ggcttcgacc
       61 tcgaacttta ataataagtt agatgttttt gaagaaaaga ctaagcatgc aagattaaac
      121 tttgaaacaa ctgcaaagca ctatattaaa cgacttgatg aagataattt gaaattagat
      181 tttcaacaag ctattcaaga tgaattaagc gatactaaag acgaaatacg tgaagtgacg
      241 aaacaagcga gagaagaaac aaaagagtat aaagaaatct tagaaagtaa aataaaagac
      301 cataacaaag tggttgataa atcgaataca gcgctaaaag tgatgacaaa aggcgttaca
      361 aatatatttt ttgtgctaat tatttttgta cttgtaatgc ttgtgacagg tccgataggt
      421 catttctttg gtattgaaca tttatatagc tttattaatg gttttattga tgaccacgaa
      481 agtgcatggc gatatttgat gttgatactt tatgcagtac cctatgtatt cttcgctatc
      541 attctgtggc tcatagcatt gttttttaga gtatttaatg acctttaggt agatttatat
      601 ataaaaaggg ctaaatacgc aaaatacccc cttaaaatgc aaaataaatg caaataaggg
      661 ggtaactcct tatgaaaaaa caactaatag tattataaca cgtttttatg ccgagaaaac
      721 ttattggttg gaatgggcta tgtgttagct aacttgttag cgagttggtt ggacttgaat
      781 tgggattaat cccaagaaag taccaactca acaacacata aagccctgta ggttccgacc
      841 aataaggaaa ttggaataaa gcaacgaaag gagttgagta aaatgagtga gaaaacgaag
      901 aaaatcattc gagtatcatt agctttttca caagtagtag tgcaatcatt gatagtttat
      961 tatacttacc agcaatataa attatctaaa gagaattaag ttttgattat gtaataagtt
     1021 ttggtactaa aaaagacttg atctaattag accaagtctt ttgttagtgt tatattaata
     1081 acaaaataaa aataagaagt cgctcactcc ccgaccaaag tttgtgagca aaataaacag
     1141 tcgatgtttg tttcttaatt gtatatcttg atattaaacg atttaagatt attcttcaag
     1201 atatatattc gcaaatgtaa ttggaaaagc gacttcttaa acaaaattaa ggagtcgctt
     1261 tttatggcaa aaataacgaa aaatgagcaa gaaaatttgg tggaaaatta cacaaaaaaa
     1321 ccggcgtact ctaatagccg gttagacgca catttcgtgt gcacttctaa tccgaaattg
     1381 agctttgacg ctatgacgat agtcggaaac cttaataaaa acagcgctaa aaagctatcc
     1441 gattttatga gtttagatcc acaaattaga ctttgggata tacttcaaac aaaatttaaa
     1501 gctaaagcac tacaagaccc cctatgttag gctagttgtc gtataggata ataagttata
     1561 ataaagacta tacggaggat gattaccatg acaaataaaa acaatgaacg ttactctacc
     1621 gaactgaaag cgacagccat caagaaaatg atgccacccg aaaatagggg gattcgccaa
     1681 ttaagtcaaa ctttgggcat tccagaagcg actctttgtt cttggcgtaa aaaagctaga
     1741 attcaaggga atactacacc cggaacgaaa aaaaatgtcg aaaaatggtc aagtgaagat
     1801 aaatttttaa ttgtgatgga atcattctca atgaaccaag tagaacttgc ggaatattgc
     1861 cgaaagaagg gcttatttaa agaacaaatt gaggcttgga agaaaacgtg cttatctgcg
     1921 aatgaacagg aagaaaatcg aacgagagaa cttgcaactg aactaaaaga agagaaaaaa
     1981 caagcgcgtc aattagagaa agaattacgt cataaggaaa aagcattagc ggaagccgct
     2041 gctctcttat tactaagaaa aaaagcacac gcgatttggg gggaccaaga ggaagaatga
     2101 tttcatcttc agatcgcaaa caagctgttg aacttattca agaagccaat cgcaatgggg
     2161 cccgtctcac ctatgcctgt aatgagctga atattagcgt tcgaacctac gaacgatgga
     2221 cgcgtgaagg tacgatagcg catgatcaac gccctttggc gaaacgtcct gtacccaaaa
     2281 ataaactgac ggatcaagaa cgcgaaaaaa taatagaaac cgtttcaaaa aaagaattta
     2341 tgaatttgcc acctagccaa attgtaccaa aacttgcgga ttgctcgatt tatattgcat
     2401 cagagtctag cttctatcgt gtattgcgag aaaaaaatat gcaacatcac cgtggccgaa
     2461 gtcaagtttc acaaaaacgc ataccgccaa gtcaccttgc gacgaagcca aatgaagttt
     2521 ggacttggga tattacttgg ctaaaaggtc caataaaagg actcttttac cggttatatc
     2581 tcatcattga tttatttagc cgtaaaatgg taggctggga gatttgggaa tcagaagaag
     2641 ctcgtcatgc cgaaaaactt attaaacaga cggtgcttaa ggaacgtatc caaggacagc
     2701 cgttggtttt acactccgat aatggcagcc ctatgaaagc cgcgactttt caagtcttac
     2761 tcgaaaaatt aggaattcaa agttcttatt caagaccacg agtgagtaat gacaatcctt
     2821 attcggaagc ggctttccga acgttaaaat atcgaccaga atatcccaat aacggcttta
     2881 aaacaataga tgatgctcga aaatggacga aagagtttgt cgattggtat aacgattctc
     2941 atttgcatag tgggattcaa tttgtgtctc ctgcagcgtg tcatgaagga cgacatctcg
     3001 ctattttaga gaaacgaaag caggtttacg aggacgctaa aaagcgccat cctgaacgct
     3061 ggtcaaaagg tgttcgaaat tggactccac atcaatcggt ggcgttgaat cctttaaaag
     3121 aaacagaaag ttaacaggag cataatcttt caagattatc aaataaccct cgtcacccaa
     3181 taggggaaaa agttattctt cccctattgg ataacgaggc caaccgacca tagggaggtt
     3241 ctgctttttg ataggtggaa aataaaaata cgacaactat attgacaaac ac
//
LOCUS       DACKAC010000130         3232 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.135, whole genome shotgun sequence.
ACCESSION   DACKAC010000130 DACKAC010000000
VERSION     DACKAC010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.135"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            184..1138
                     /locus_tag="I0B46_002713"
                     /pseudo
     CDS             184..1138
                     /locus_tag="I0B46_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 105 protein"
     gene            1155..2179
                     /locus_tag="I0B46_002714"
                     /pseudo
     CDS             1155..2179
                     /locus_tag="I0B46_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            2185..2664
                     /locus_tag="I0B46_002715"
     CDS             2185..2664
                     /locus_tag="I0B46_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAR2084951.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKARLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            2680..>3232
                     /locus_tag="I0B46_002716"
     CDS             2680..>3232
                     /locus_tag="I0B46_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAR2084952.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVG"
BASE COUNT          980 a          539 c          751 g          962 t
ORIGIN      
        1 acttggaaaa cgttgtttct ccaagttctt tttttaattt ccaagtaact gaaagttact
       61 tttgttattt aacatttagg tcaattttag acagtttttg ttcaaaaagc gatatttata
      121 gtggacggta ttactgatat attaaggata taaaatgaaa gcgtttaaaa aggtggcgta
      181 aagatgatta ttaaagaaga gatttttgta cctaaagaat tagtgaaaga aaaaattgac
      241 ctgctcgttg aaaacctgac aacgatcaaa gacgataatg gtgaattttt gttgggcttt
      301 gatggactga aagtagatga caagagctgg acagtgtgga attggccaca aggtgtcggt
      361 ctatatggta tttataaaaa ttaccagatg acaaaaaatc ctcgcgctta tcaagtggtc
      421 aatgaatggt ttgaggatcg tatggaagaa ggcgctccgc ccaaaaacgt caatacaatg
      481 gcaccgctat tgactatggc ttacttgtat gaagacacga aagattcacg ctatttgcct
      541 tatttagaac aatgggcaga atgggtcatg aatgacatgc ctcggacaaa tgaagacggg
      601 ttacagcatg ccacatatgg accggaaaat aagaatcagt tgtgggacga tacattgatg
      661 atgacagtgc ttcctttggc aaaaatcggg aaactgctgg ttgacgattg ctattccaga
      721 gatcattgaa atcttagaat tatcgaaaga agatgcattg cgcaaattat tgatcgaaac
      781 acttgaagca caagtgcgtg cattaaaaac ttatcagtct gaaagcggtt tatggcatac
      841 gttgttggat gatccttctt cttacgtaga atcttctgca actgcaggat ttgcttacgg
      901 tattttgaaa gcggtccata aacgttattt accacaagaa tataaagaag tcgcttatcg
      961 tgctatccaa ggattgcttg aacaaatcga tgaaaaagga gaagttcaaa acgtttcaat
     1021 cgggacagga atgggggaca gtttagattt ttatcggaat atcggaatca cagcaatgcc
     1081 ttatggacag tcgttaacgg tgctttgttt gacagaattg ctcgtctctt attgttaaaa
     1141 gaaaggaaac ccatatgaaa aaaacatcac tgctgtttgc agcaagttcg cttagcctag
     1201 gtctgctagg tctaagcggc tgcggaaata attctagtgt ggcaactgtc gcagatgcag
     1261 acgtaaatga gatagttacc ttgcgctttg cttatgcaag caacagccag taattgatgc
     1321 aatgaatgaa tttgggcgtt tagtaaaaga aaaaacagat ggttccgtac agatccagta
     1381 tttcccagat ggacaactgg gtggagaacg tgaactgatc gaattgacac agtcaggagc
     1441 agtagatttc acaaaagtaa gtgcctcagc gttggaaagt ttctctaaag attattcgat
     1501 tttttccgtt ccttatctat tcaatgatga agaacatttc tttaaggtga tggataatga
     1561 agaaataatg gagccaatct accaatcgac caaaaattta ggatttaccg gattgaccta
     1621 ttatgattct ggtcagctca gcttttatat ggtagacggt ccgatccata cgccggaaga
     1681 tttgaaggga aagaagatcc gtgtgatgca aagcgaaacc gctatcaaaa tggtagagct
     1741 tttaggcggt tcgcccgtac ctatgggaag tgatgaagtg tacacgtctc ttcaatccaa
     1801 tttgatcaac ggatcagaaa acaatgaatt tgtcttgtat acagctggac atggcggggt
     1861 agctaaatac tattcctatg atgagcatac acgtgtgcca gatatcatca tcatgaatga
     1921 tgccatcaaa gaacagctaa ctgatgaaca ggaaaaagcc attgaagaag cagcgaaaga
     1981 atcaacggtt tttgaaattg aagcttttgc aaaagcaatt gaagaagaaa aagctttagc
     2041 acaagaagaa catggagtcc aattcaatga tgtagataat acaccgtttc gagaggcagt
     2101 tgccccactt catgatatat tcaaaaacga tcctacctac aaagaccttt atcaaaagat
     2161 ccaagagcaa ggagcgtagc tgtaatgaaa atgattttaa acaaggtatt agagatcctg
     2221 ggagctgttt tgcttgttgg gatggtcgct gttgttttat ggcaagtatt ttccagaacg
     2281 gttttaggaa atccaaatac ggtcacggaa gagcttgttc gattcggtct tgtctggttt
     2341 gccatgttgt cttccgcgta tgttgtggga caaaaagcac gtttagccgt aacggtttta
     2401 agtgaaaaat taactggcaa aaaaagtaaa attttagaag ttgttgttca aagtttattt
     2461 ctggtatttg ctgcgatgat catggtgtat ggcggatgga atgctgtcac attgacaatg
     2521 gggcagatct cgccttcttt aagtattcct atgggatatg tttatctgtc agtacctgta
     2581 tcgggggtac tcatcattat ctatagcctg atcaatctaa tcgatacatt aagcaacaag
     2641 ccagcaattc aagaaaaaat ataaaaatgg aggaataaaa tggcaataga agccggatta
     2701 gtgttatttt ttgtatttgt tgtgttgctt gtattaggga tgccgattgc tataagtatt
     2761 gcattctcat cgatgatgac tttattgtta gttataccgt ttgatgtttc tgcttttagt
     2821 tcagctcaaa aaatggtggg aagtatcaat agtttttcgc tgatcgctat tccgtttttc
     2881 gttctctcgg gcattatcat gaataatgga gggatagcta agaaactagt ggattttgcg
     2941 atgttgttcg tcgggcgcgt gccgggggct ttggctcata cgaatgtgtt agggaatgct
     3001 ttatttggtt ccatctctag ctcggccatt gccgcttcta ccgcgattgg tggagtgttg
     3061 atccctcagc aagtggaaga aggatatgac cggaagtttg cgacagcggt caatatcgcg
     3121 tcagctccga caggtatggt cattcctcca agtactgctt ttatcatgta ttcacttgtt
     3181 gctggtggtg cttcgatctc tgctttgttt atgggaggtt atctcgtagg cg
//
LOCUS       DACKAC010000131         3209 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.136, whole genome shotgun sequence.
ACCESSION   DACKAC010000131 DACKAC010000000
VERSION     DACKAC010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.136"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            116..976
                     /locus_tag="I0B46_002717"
                     /pseudo
     CDS             116..976
                     /locus_tag="I0B46_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002384409.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
     gene            988..1815
                     /gene="panB"
                     /locus_tag="I0B46_002718"
     CDS             988..1815
                     /gene="panB"
                     /locus_tag="I0B46_002718"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HAR2084953.1"
                     /translation="MKNTAVTFKESKLRNEKLTMLTAYDYSTAKIIDEAGINGILVGD
                     SLGMVCLGHEDTLSVTMEDMIHHTRAVTRGAKNTLVVADMPFMSYQTSVYDSVVNAGR
                     LIKEGRAQVVKLEGGIEVCDKIEAIVKASIPVMAHIGLTPQSVNAFGGFKVQGKDKEA
                     AKELIRAAKAVEKAGAFAVVLECVPTKLAELISKEISIPTIGIGAGAGCDGQILVYQD
                     MLGMYSDFTPKFVKKYANLSEEMNKAFTKYIEEVKDGVFPGPEHGFKISDDVLEKLY"
     gene            1830..2678
                     /locus_tag="I0B46_002719"
     CDS             1830..2678
                     /locus_tag="I0B46_002719"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HAR2084954.1"
                     /translation="MVIFKTVNDVRSQVKEWKKQGLKVGLVPTMGYLHEGHESLIRKA
                     SKENDKVVVSIFVNPTQFGKNEDLGSYPRDLERDIEVCTRGRATAIFNPEVEEMYCDN
                     ASTFVNITGLTEGLCGASRPIHFRGVCTVVSKLFNIIPADRAYFGEKDAQQLAVIKRM
                     VRDLNIDIDVVGCPIIREEDGLAKSSRNTYLSLEERSSATILNKSLTLAKEALNNGER
                     DSLKIIEIISKNINTYNLAKIDYVEVVDSLSLQRVNYIEKSVLVAIAVFIGKTRLIDN
                     FTFELQ"
     gene            2688..3083
                     /locus_tag="I0B46_002720"
     CDS             2688..3083
                     /locus_tag="I0B46_002720"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HAR2084955.1"
                     /translation="MKVTMLKGKIHRATVEQAELDYVGSITVDMNLLEAAGIYEYEKV
                     QIVDVNNGNRFETYTIAGERGTGMICLNGAAARCVSTGDKIILMAYCELNENEVKDHR
                     PKVVFVDDNNKVERVTSYEKHGRLSDIIL"
BASE COUNT         1246 a          326 c          642 g          995 t
ORIGIN      
        1 gatattatga taaattaact atggatgata tttgaaaaaa acatactgta tatttgattt
       61 aatttttatt tttaagctca tatcgtaaga atgggacttg tagtctcact tctttatgga
      121 ggtgataatt atgaacgttg gttttattgg agccggcaag gtaggttgtt cattcgggaa
      181 gtatttccag gaacataaaa tacaagtaac aggattttat agtaaaagcg aagactcgtc
      241 attagcagct tcgaatttta catctagtaa acaatattta aacttaagag aattagtaga
      301 tgaaaatgat actattttca ttacgactcc tgatggtcaa attcaggagg tatggcaaga
      361 aattaaaaat tattaaatca aaaacaaatt aatctgccat tgcagtggtg caatatcttc
      421 agatatcttt tcaaatatac aggactatgg tgcttatggc tattctgtcc atcctatgtt
      481 ttcaatatca gataaatata attcatacaa aaaattaaaa aaatctttta taacaattga
      541 aggcgatgaa aaatatgcaa agtttttatg tgatttcttc aaaaagttag gtaatagtac
      601 tgtaattgta tctaaggaga ataaatctct ttatcatgcc gcatcagtag tatctagtaa
      661 ccttgtgcta ggacttatta atacaagcgt tacatatcta attcaatgtg gatttactga
      721 aaaaatggca attgaagctt tatatccatt aattgaattt aatattagaa atataaaaga
      781 aaaaggaatt atagaaagtt tgacagggcc agttgaacgt tgtgatattt caacaattaa
      841 aggtcactgt gaagttttat cagatgaaga cagagcttta tatatgctat tatcaaaaaa
      901 tgtattagag atagctatgt taaaaaatat taatagagat tatagtgaat tagaaaagta
      961 tttaggagag agttaggaga gaaatagatg aaaaatacag cagttacatt taaagaatct
     1021 aaattaagaa atgaaaaatt aacaatgctt acagcttatg actattcaac agcaaaaata
     1081 attgatgaag ctgggataaa tggaatatta gtaggagatt ctcttggtat ggtatgtcta
     1141 ggacatgaag atacactttc agtaacaatg gaagacatga ttcatcatac tagagcagtt
     1201 acaagaggag ctaagaacac tttagttgtt gcagatatgc catttatgtc ttatcaaact
     1261 tcagtatatg attcagtagt taatgcagga agattaataa aagaaggacg agctcaggtt
     1321 gttaaattag aaggagggat agaagtatgc gataaaatag aggctatagt aaaggcttct
     1381 atacctgtta tggcgcatat aggtttaaca cctcaatctg ttaatgcatt tggaggattt
     1441 aaggtacaag gaaaggacaa ggaagctgct aaagaattaa taagagctgc taaagctgta
     1501 gaaaaagcag gagcattcgc tgtagtgtta gaatgtgttc caactaagct tgcagagtta
     1561 atttctaaag agatatcaat acctacaatt ggtattggag caggtgcagg gtgtgatgga
     1621 caaattcttg tatatcaaga tatgttgggg atgtatagtg attttacccc taaatttgtg
     1681 aagaaatatg ctaatttatc agaagagatg aataaagcat ttacaaaata tatagaggaa
     1741 gtaaaagatg gagtttttcc aggtccagaa catgggttta aaataagtga tgatgtttta
     1801 gaaaaattat attaaaaggg tcaagaaata tggttatatt taaaacagtg aatgatgtta
     1861 gaagtcaagt aaaagagtgg aaaaaacaag gcttaaaggt aggattagtt cctactatgg
     1921 gatatttaca tgaaggtcat gaaagtttaa ttagaaaagc atcaaaagaa aatgataaag
     1981 ttgttgtaag tatctttgta aatccaactc agtttgggaa aaatgaagat ttaggtagtt
     2041 atccaagaga cttagaaaga gatatagaag tatgcacaag aggtagagca acagcaatat
     2101 ttaatcctga agttgaagag atgtattgtg acaatgcaag tacttttgtt aatataactg
     2161 gattaacaga aggtttatgt ggagctagta ggccaataca ttttagaggg gtttgcactg
     2221 ttgtaagtaa attatttaac ataataccag cagatagagc ttattttgga gaaaaggatg
     2281 cacaacagtt agctgtaatt aaaagaatgg ttagggacct taatattgat atagacgttg
     2341 tagggtgtcc aattataagg gaagaggatg gactagctaa aagttctaga aacacatact
     2401 taagtttaga ggaaagatct tcagctacta ttttaaataa atctttaact ttagctaagg
     2461 aagctttaaa taatggagag agagacagct taaaaataat tgagataata agtaaaaata
     2521 taaatactta taatttggca aagattgatt atgtagaagt tgttgattct ttatcattac
     2581 aaagagtaaa ttatattgaa aaatcagttt tagttgcaat agcagtattt ataggaaaaa
     2641 caagattaat agataatttt acatttgaat tacaataggg ggctataatg aaagttacaa
     2701 tgttaaaagg aaaaatacat agagctacag ttgaacaagc tgagttagat tatgtaggaa
     2761 gtattactgt tgatatgaat ttattagaag cagcaggaat atatgaatat gaaaaagtac
     2821 aaatagttga tgttaataat ggaaatagat ttgaaactta tacaatagct ggagagagag
     2881 gaacagggat gatttgcttg aatggagctg cagctagatg tgtatcaact ggagataaaa
     2941 taattttaat ggcatattgt gaattaaatg aaaatgaggt taaggatcat agacctaagg
     3001 ttgtttttgt cgatgataat aataaagttg aaagagtaac atcatatgaa aaacatggaa
     3061 ggttatcaga tataattctt taggttttgt aatgttattt atatatagct agactattat
     3121 tgaaatattt attgattact aattggatag atgtcaataa agtagacaca aaaagagaag
     3181 atacagactg tagacaaagt ccattttat
//
LOCUS       DACKAC010000132         3113 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.137, whole genome shotgun sequence.
ACCESSION   DACKAC010000132 DACKAC010000000
VERSION     DACKAC010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.137"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            186..1646
                     /locus_tag="I0B46_002721"
     CDS             186..1646
                     /locus_tag="I0B46_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAR2084956.1"
                     /translation="MTEKKMSLIDRCKQMDIVDFARNNGLAVANKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPDWLIDYTEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPREYKVI
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEVISLKD
                     ICREVAKELKDCERSNGTYDFDRFYQGKGDINSQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIHQQEIRKQKRAMLFQQGRQQERE"
     gene            1657..>3113
                     /locus_tag="I0B46_002722"
     CDS             1657..>3113
                     /locus_tag="I0B46_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAR2084957.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFNVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVTAQNGIFDAKLDPNETQATQEAFEAYLKEKGVQVGKQPGTILEVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAESIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ENDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPP"
BASE COUNT         1123 a          556 c          666 g          768 t
ORIGIN      
        1 taccttcaga agaatttgcg ctaggaaatc cattccgatt ttcacttgag acagtgtcag
       61 aagtggcaaa acgaaaaaat gaaatctatt atgaacaaca attgtcacaa aaagaacgcg
      121 tataaaacaa gcaagaaaga ctatctgaaa aatggatagt cttttttctt ttgggaggtt
      181 tgaaaatgac agagaaaaaa atgagtctca ttgatcgatg caagcaaatg gatattgtgg
      241 attttgcacg aaacaatggt ttagcggtgg ccaataaagg acgagactat cgcttagaag
      301 accatgattc atttgttttt gatcgaagaa aacaacgttt ttattggaac agtcaaaata
      361 ttagtggcga cattattgaa ttagctaaac tttttttcat agacaaagag atccaagacc
      421 ctaagcaaca atttaaagca gccactgatt ttattctaaa gaatgaagac aaaacagaac
      481 gagtggaaaa tcttcatttt gagacagaaa agtacaaaga tcaccccgtt gattatcaac
      541 cactcacaga aaaaggaagg aattatctga aggaagaacg aaaactgccc gattggttga
      601 ttgattatac agaaaaagaa ggattgattg ccgaactaaa gccaaaacat gaacggcaaa
      661 atttcttagt tcgagacgat cgtttggatc atgcggtcgc ctttttatgg aaagatccac
      721 agacaaaaga gacagtaggg gcgagttatc aaggaacgtt cattgattat gaacgttttg
      781 gtgagagagg cacatataag catatagata aaaattcgac agcgaatcat ggctttaacc
      841 taaagattgg tgatcctaaa cagttaaaat tttttgagag tagtattgat ttacttagct
      901 acgctgcact gaatcgtgat cagttaaacg atacgtggtt agtctctatg gaagggttaa
      961 aacaccatgt gatcagtcat tattttggtg aggcagtatc tgaattgaga aagaaacaag
     1021 cctttcctca atccatcgag atttgtgtgg ataatgaccg tgcaggacat attttctacg
     1081 aaaaagaaca attaatgggc gcagttgatc catttacgaa tcagaaagta cgttgtgaac
     1141 gaggaatcgc taatgattgg caagtgccaa gagaatacaa agtcatttat gaagaagtcg
     1201 caaaagaaga aaaggtcaca ccagaagcta ttatggccat acacaagaca gaaaacaatc
     1261 tgcaattaac gaaccaactg gtttccgctc acaaagtcaa tgcgtctttt ggtcagcagc
     1321 tttctgttaa cgattcaata gaagtcatta gtttgaaaga tatttgtaga gaagtcgcaa
     1381 aagaattaaa agattgtgaa cgctcgaatg gtacgtatga ttttgatcgt ttttatcaag
     1441 gaaaagggga tattaattct cagattcttt tctcttataa agcagaacaa tactacaaag
     1501 gctataaaaa tcatgaacat gaatttgtac ctgaagtgaa aaaggattgg aacgatcaat
     1561 tgaagcatga gatccatcaa caggaaatta gaaagcaaaa acgtgcgatg ttgtttcaac
     1621 aaggcagaca acaagaaagg gagtgagatc gtcacgatga aaactgtcat tttagcagaa
     1681 aaaccgtcac aagcgaaggc ctatgccgat agcttttcta aagccacccg aaaagacggg
     1741 tattttgaaa tccaagatcg attgttttcg ggtgaaactg tgatcacgta tggttttggt
     1801 catttagtcg agttagattc gcccgatatg tacgatgaaa attggaaaca atggtcatta
     1861 gagcatttgc ctattttccc tacacattat cactatcatg ttcctaaaga taagaaaaag
     1921 caattcaacg tggtgaaaca gcaacttcaa tcggccgaca ccattattat tgcgacagat
     1981 agcgatcgtg aaggggaact gatcgcttgg acgatcattc aacaagcagg cgctgatcaa
     2041 ggaaaaacct ttaaaagact ttggatcaac tccttagaaa aagaagccat ttatcaggga
     2101 ttccagcaat tacgagacgc agaagagacc tatcccaaat ttgaagaagc acaagcacgc
     2161 cagatcgctg attggttgat cggcatgaac ggcagcccac tgtatagttt actcttgcag
     2221 caaaagggca taccaggaag tttttcctta ggcagagtcc aaaccccaac gctttacatg
     2281 atttatcagc tgcaggaaaa gattagaaac ttccaaaaag agccttattt tgaagggaaa
     2341 gcccaagtaa ccgctcaaaa tggcatattt gatgcgaagc ttgatcccaa tgaaacccaa
     2401 gcgacgcaag aagcatttga agcctattta aaagaaaaag gtgttcaagt aggaaagcag
     2461 ccaggcacga ttcttgaagt cgaaaccgag aaaaaaagcg cggcaagtcc acgtcttttt
     2521 tctctatcca gccttcagtc taaaatgaat cagctcatga aagctagtgc caaagatact
     2581 ttggaagcca tgcaagggtt gtatgaaggg aaatatctaa gctatccacg aacagacacc
     2641 ccttatatta cggaagggga gtatgcgtat ttgttggatc atttagatga atacaagcat
     2701 tttttaaagg ccgaatcaat cccaacccct attcatacgc cgaatagtcg gtatgtgaat
     2761 aacaagaaag tccaggaaca ttacgcgatc attcctacaa aaacggttat gacagcagcg
     2821 gcttttgaac aactaagtcc actgcagcag gccatttatg aacaagtgtt aaaaacaacg
     2881 gtggccatgt ttgcggaaaa atatacctat gaagaaacaa cgatcttgac gcaagtccag
     2941 cagcttcagt taaaggcaat cggaaaagta ccgatagatc taggttggaa gaagttattt
     3001 ggcaaagaga acgacggaaa agagaaggaa gaagagccgc tacttcctaa agtgacaaaa
     3061 ggagaaatgg tgacggtaga tctacaagtc cttgaaaaag aaaccaagcc acc
//
LOCUS       DACKAC010000133         3012 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.138, whole genome shotgun sequence.
ACCESSION   DACKAC010000133 DACKAC010000000
VERSION     DACKAC010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.138"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(255..515)
                     /locus_tag="I0B46_002723"
     CDS             complement(255..515)
                     /locus_tag="I0B46_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084958.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            complement(881..1366)
                     /gene="ssb"
                     /locus_tag="I0B46_002724"
     CDS             complement(881..1366)
                     /gene="ssb"
                     /locus_tag="I0B46_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAR2084959.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFSLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(1369..2268)
                     /locus_tag="I0B46_002725"
     CDS             complement(1369..2268)
                     /locus_tag="I0B46_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAR2084960.1"
                     /translation="MTPTQFKEKTRNFAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTANPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAYLENARVPFKLDITSGDSIIPAALMYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERIDEKNLYKSVQGTFKE
                     RNNQSVFKHLYDQEMPQLVKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEKAERKQNFKRSQQMNQEMER"
     gene            complement(2284..2877)
                     /locus_tag="I0B46_002726"
     CDS             complement(2284..2877)
                     /locus_tag="I0B46_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAR2084961.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKDAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDQYAIQPHYTNKENFDLGVTETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMDILKSPKEMTLYMLPLY"
BASE COUNT          844 a          582 c          502 g         1084 t
ORIGIN      
        1 aagagatagc gaacaggagc gaaccttttg gtgagaggaa gttttttcta ttttgtcttg
       61 ctttgcaaga ccccttatct tagtgaacgt gaaacgttca cttcctttct tctcgaacgg
      121 cggtggcggt tggagtgaga gaaatgtttt tcaattgaga aggcgcttgt gccttcctaa
      181 aaaggtcttt tcctgtttct atgtaaaaca aaaagagctg caatcctcat ttaggactac
      241 aactcttctt ttatttatat gcccatcgat cgcgtaaaat gatcgggatt tctaacatac
      301 atgctagcaa taactagctg atttcgttta ttgaagtgac ctgtgacggc aatattgtat
      361 tttccatcat ccataattag caatttatcg ataatttcaa tatctgcaac tacacaatta
      421 atcgattcat gaatcgtttg taaagtaaag cgaaccaata acggttgcgt ttttattact
      481 cggattttat caagcaaacc aacatatttg ttcatcacaa tcacctcgct aaaattatag
      541 caaaaatcca atcatttgga tatttcaatt atgctataat agtcttgctg tctgctaccc
      601 cggcctaatc tggcaagggg aggaggttat aggtttgaaa attctttttg attttgtttt
      661 tgcaatcatg gcagaagtag ccgcgcacta tatctgcaaa tggcttgatt gtaaatttgc
      721 caagaaagac agtaagccta cccgttaatt cttataatag aaacgtaaaa gaaagccact
      781 agcccgccag ctagtggttt ttggttatag attgaaaatt ctttttgttg tctaaattat
      841 aacataaacc gtagcaaatg caaccaatag aaaatgctac ttagaacggt agatcatcat
      901 cgctaatatc gattgattgt ccaccaaatg gattctgtga agcgttcaat ccattgttgt
      961 taggcgtcgc tgtatcccgt tgttcaaagg tggtagggct tggactgctg gacgattgat
     1021 cagcgtgcat acggctttct gtcgctgctt tactttctaa tagttggaag ttatcggcca
     1081 caacttctgt cacatacaca cgttgtcctt gctggttgtc ataagtccgt gtttgaattc
     1141 gtccgacaac gccgatcaag ctgccttttt tagctaatgt cgccatcgtt tctgctggtt
     1201 tgcgccaaat cacgcaattg ataaaatcag cttctcgatt gccgttttga ttcgtgaaat
     1261 tacgattaac ggctagggaa aaagtcgcca ctgctgtgcc acttgcggta taacgtaaat
     1321 ctggatcttt agttaatcgc cctactaaag ttacattatt gatcatcttt atctctccat
     1381 ttcttgattc atttgttgtg atctcttaaa attttgtttt cgttctgcct tctcttgctc
     1441 tacaattgat tgcatcaggg aatcaacgga agttagtgta gattcgaaag aaatgtcttt
     1501 ggcaaaatag ttttcttgtt ggtatctttt ccacttttct ctcaagccct catctttcac
     1561 caattgaggc atttcctgat catacaaatg cttaaacacc gattgattat ttctttcctt
     1621 gaatgttcct tgaacactct tatacaaatt tttctcatcg atacgttcta atttcggaat
     1681 catatacagg tcataaaaat ctttcatgcg cgaattatct tttccaaaac taaacgtggc
     1741 atgtaatttt tcagctagaa tttgttccgt aggataagct ggaatatgaa tcgttttatc
     1801 ttcaaacatt aatttgtgtt catacatcaa tgctgctgga ataatagaat cacctgaagt
     1861 aatatctaat ttaaaaggta cacgtgcatt ttcaagataa gcaataaact ttgcttgaaa
     1921 acctggataa taatcggctt ccctcgtctc tgttaatccc tttaattcaa aacgaattcc
     1981 atcttttgta ggattagctg ttaattcaga aaaaacagtt tctaactttt cttttgttag
     2041 tttcgaattt cgataagtcg tatcaatatc aacggttgaa cggttttcta acccgtattt
     2101 tgatccaatc agaaatcccc cttttaatac aaaatcatca tgataaggcg attcaacaat
     2161 acattctaat aatttttcca tcatataata tctttggaca atttgtgggt ttaaatgttt
     2221 ctctgctgcg aaatttcttg ttttctcttt aaattgtgtc ggtgtcattc atctttactc
     2281 ctctcaataa agtggcaaca tatacaaagt catttctttt ggtgatttta aaatatccat
     2341 gtatcttctt agcttatttg tatttctact ggtacttgtc atatattctt ttaatgcttc
     2401 ttgttttacc tctacgcttg ctttgtatct tggattccac atgtcacata acgttcgctc
     2461 taaatcatag actaaaattt tattgccgta aaagctttct gtttctgtga ctcccaggtc
     2521 aaaattttct ttattagtgt aatgcggttg aatggcatac tgatctaatt tctcctttga
     2581 tacattgtat cctttaggaa acatcatatt gatcttttta gggatatatg ttcctacttg
     2641 aaaaagatcg agcgcagtct catgagaaaa aatccctctg gaatagatac tctgagcgag
     2701 atacaattca tccacgaacg catcctttaa ggtatagaaa cctggtgcta ccttttctat
     2761 tttatccttt tcggctaatc gatctaacgt cataggcgat aatccttcac ttctagcatc
     2821 cttcaaggat aggttgccat tatgtttatc aaaaatacat tggattttgt ctttcattac
     2881 tcttccccta aagttaatat aaaactaaca caactataac atgttagttt tatattaaca
     2941 agaaaggaat ttatcgatct aattcgattt ctttttgaat ccgctgttgg ttctttaatt
     3001 gctgcagtct tc
//
LOCUS       DACKAC010000134         2840 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.139, whole genome shotgun sequence.
ACCESSION   DACKAC010000134 DACKAC010000000
VERSION     DACKAC010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.139"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            160..627
                     /locus_tag="I0B46_002727"
     CDS             160..627
                     /locus_tag="I0B46_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HAR2084962.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQPR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            902..1138
                     /locus_tag="I0B46_002728"
     CDS             902..1138
                     /locus_tag="I0B46_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084963.1"
                     /translation="MYWRVYKMKIASEKIDQLTILVNQRIEDIIKEFEGEKIKNIKLT
                     LMDHKFELNSTKRKIVELIEEEIALQINEQQLAN"
     gene            1162..1503
                     /locus_tag="I0B46_002729"
     CDS             1162..1503
                     /locus_tag="I0B46_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084964.1"
                     /translation="MEVEQIKYQGMDRERLIDIIEQQRLELIQKEAVCKEYKKHLEQV
                     IEYHSVEKYRSVVQKNRATSPDSRQDVSISIKTPVFNSVKSEKLESISTALVEKAAEM
                     NSLANEVSRLI"
     gene            complement(1496..1675)
                     /locus_tag="I0B46_002730"
     CDS             complement(1496..1675)
                     /locus_tag="I0B46_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HAR2084965.1"
                     /translation="MYFVIREATNGQYYFVIKSNNNEVVATSETYLTKYSAEQTINSI
                     KNGITKDSQVIDMTK"
     gene            1897..2544
                     /locus_tag="I0B46_002731"
     CDS             1897..2544
                     /locus_tag="I0B46_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084966.1"
                     /translation="MVGVVKFLGFTFDGYGTLGEWVGIILIVITIQQTSKYYWYSNSS
                     KIVSRITEGITKKDITVVGKYGFTITLLNEGKSETAIKFCGIIGKYNIFQRFIRKHWY
                     KYIYKYPVVKKLIEKYVDHEISYLNPTDLINQEFQNIGYLEKGLKIPIVNESLVTAMV
                     ANIKNSKKLEKRYKKCKKLKFSFLFLEYNGKEYYQDIYVLRGSNLYKDIKNKISV"
BASE COUNT         1045 a          395 c          542 g          858 t
ORIGIN      
        1 agaaaaagac agaatgaagg cagtagcttt ggttgaattt gctgaggcgt ataaatcagg
       61 agaactcatt tcacgtacaa aataaaaaaa gtcggattcc tccgactatg aataatattt
      121 ccgacataag tattatatca taattggggg aatcagagga tggtactttt cgatgtaaag
      181 aaatatgaga caccaagcgc gaaagatgtt gatatggaac gcacaaaaca taatgtcgct
      241 gtgtttcttt cagcatatct atcagctaga tgtagagtag ggcaacctcg agaaccaaaa
      301 gtgacagcat cttattcctt ggttccacct tctacagctg atcatatatt tgaagcagaa
      361 agaatgctga tcgataaaga agaagcacag gaagaatttg agtatctgca taaattgttt
      421 gttcgaggct attcagcgat acagcatccg cataagcctg atgtgactga aaggcgcaag
      481 aagatattct atgatcgtta tatcaatggt ctgcccattt atgtaactgc tcaaaggaat
      541 aatactagcg aagaatcggt taaagtagaa tcaaacagaa ttatcatcca atttgcttca
      601 tcgttagaac tggttgcttt caagtagcca gtttttacac tttttatacc cttttattac
      661 caatttggtt tccattttat acctttttta taccaatcac ttacctattc aacgttgtat
      721 tatgatagtg tcgaaagatt aggaaacagg atcgacaaaa taaaatgtaa gggaggaaat
      781 ctccctcatc gtttaattaa gcttcgatag gcagcagcgg aaatattaag aataaggatg
      841 tgaatttcaa ctccttctaa attgttctta ttatctatca tccgttgctg tctattaatt
      901 tatgtattgg agggtatata aaatgaagat cgcttccgaa aaaattgatc aactaacaat
      961 tttagtaaac caaagaatcg aagatattat caaagaattc gaaggtgaaa agattaagaa
     1021 cataaagctt actttaatgg atcataagtt cgaattaaat tcgacaaaaa ggaagatcgt
     1081 tgagcttata gaagaagaaa tagctctcca gatcaatgag caacaattag ctaattaatt
     1141 tatgtattgg aggggaaatg aatggaagtc gaacaaataa agtatcaagg tatggatcgg
     1201 gaacggttga ttgatattat tgagcaacaa cgtttagaat tgatccaaaa agaagctgta
     1261 tgtaaagagt acaaaaaaca tttggaacaa gtaattgagt atcattcagt agaaaagtac
     1321 agatcagtgg tgcaaaaaaa tagagcaacg agtcctgatt ctcgtcaaga tgtatcgatt
     1381 agtatcaaga cgccagtatt taattctgtt aaatcagaaa agctagaaag tatctcaact
     1441 gctttggtag aaaaagcagc tgaaatgaac tccctagcaa atgaagttag tcgtcttatt
     1501 tagtcatatc gataacttga gagtctttgg tgattccgtt ttttatagaa ttaatggttt
     1561 gttcagcgga atatttagtc aagtatgttt cgctagttgc tactacttca ttgttattgg
     1621 acttaataac gaagtaatat tggccattag ttgcttctct tataacaaag tacaaatttt
     1681 tcaccaccta taatttattt cagcggacca ctcgctgata attaaaatta taagctatgt
     1741 ataaaatttc acaatatcaa tttgtcgctg tggcggaaag ggtagacgca ataaaggatg
     1801 aatgggaaaa gtcaatgagt aattatctga aatttacaag tcccctgttt agttatctaa
     1861 gattatttga taatattatt tctgaggtga tagaaaatgg tgggcgtagt taaatttctt
     1921 gggtttacat ttgatggata tggtacgtta ggcgaatggg taggtattat tttgatagtg
     1981 attacaattc aacaaacatc aaagtactac tggtattcta atagttctaa aattgtatca
     2041 agaataactg aaggaataac caaaaaagat attactgtcg tcggtaaata tgggtttact
     2101 ataactttat taaatgaagg gaaatctgaa accgcaataa agttttgcgg gattataggt
     2161 aaatataaca ttttccaacg ttttatcaga aagcattggt acaaatatat ttacaaatac
     2221 cctgtagtta aaaagcttat tgaaaaatat gtggaccacg aaatatctta tctcaatcca
     2281 acagacctta ttaatcaaga attccagaat attggatact tagaaaaagg tctaaaaata
     2341 ccaattgtaa atgaaagttt agttactgca atggtagcta acataaagaa cagcaagaaa
     2401 ttagaaaaga gatataaaaa atgtaaaaaa ttgaaatttt cctttttatt cttagaatat
     2461 aatggcaaag aatattatca agatatttat gttttgcgag gctcgaactt atataaagac
     2521 attaaaaata agatttcagt atgagatttt ttaaaattcg tagatcactc tttgagtggt
     2581 ctttttattt tgcgtaaagg aggcacgacg atgtatagac cacaatactt agaacagaag
     2641 catgaagtaa tcactgtgca aaacggtaac ggtgagatag tacgaaagta tagaagacca
     2701 ataaagagcg atacatataa acggaaggaa agcaatgaag ttattccatt gtatggcaaa
     2761 agaatagcta agtattaaat aagattgcga aaggagatgg gacatgaccg aggaattcta
     2821 tagatggtta ttacagttga
//
LOCUS       DACKAC010000135         2778 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.140, whole genome shotgun sequence.
ACCESSION   DACKAC010000135 DACKAC010000000
VERSION     DACKAC010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.140"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..197
                     /locus_tag="I0B46_002732"
     CDS             <1..197
                     /locus_tag="I0B46_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084967.1"
                     /translation="WFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLIIAIIAVGLV
                     FNAAGVKDILLELFNRIIGA"
     gene            314..811
                     /locus_tag="I0B46_002733"
     CDS             314..811
                     /locus_tag="I0B46_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAR2084968.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSISELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMSDVARYYIEETGALGEVPASLQNYIDYESYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            898..1290
                     /locus_tag="I0B46_002734"
     CDS             898..1290
                     /locus_tag="I0B46_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAR2084969.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAV
                     MILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRRKLTY
                     AGKKVTLGRNQPQEAITAVRSEFYGISN"
     gene            1274..>2778
                     /locus_tag="I0B46_002735"
     CDS             1274..>2778
                     /locus_tag="I0B46_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HAR2084970.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSILSAQERSKNEVTGKLKTVAYDKIDQQTDALISM
                     IGENQVDYRFFIGFKLLLNDQEFSMKSLTVEAKNAFTDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYPLSKKKLEH
                     ETLIKTYDLIKPTRCLVEEKQRYLKIQQEDETAYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKA"
BASE COUNT          839 a          518 c          596 g          825 t
ORIGIN      
        1 actggtttct atcaaacgct caatcactgg tgcttttagc aatcgttgtg attggcttgt
       61 atcttggctt caagcgtgag tttagcaaac tgattggctt tttaattatt gcgattattg
      121 cggttggctt agtcttcaac gctgctggag taaaagacat tttactagag ctattcaatc
      181 gcattattgg tgcttaaata aaaccgttct tttgtggaat ataagtggtt ttcttatgtt
      241 ccgcaaagga atggtacacc aaacgaagtg cggtagggat ttttgaatct ctacaaagaa
      301 aggacgtgaa tatatggacg atatgcaagt ctatattgcg aatttaggca aatacaatga
      361 gggcgaattg gtcggtgcgt ggtttacctt tcccattgac tttgaggaag tcaaagagaa
      421 aatcggcttg aatgatgaat atgaggaata cgccattcat gattacgagt taccctttac
      481 ggttgacgaa tacacttcta ttagcgaact caatcgccta tgggaaatgg tatcggagtt
      541 gccagaagaa ctacaatcgg agctatctgc tctgctcact catttttcaa gtattgaaga
      601 actaagcgaa catcaagaag atattatcat tcattccgat tgtgatgata tgtctgacgt
      661 ggcacgctac tacattgaag aaacgggtgc tttaggcgaa gtgccagcca gtcttcaaaa
      721 ctatattgat tatgaatcct atggtcggga tttagacctt tcgggaacgt ttatctcaac
      781 caatcacggg atttttgaaa tcgtctatta aatctgtcgg tacattctta ctggcagatt
      841 ttcttgttta cggggtagtc aaaagactat ccccattttt atgaaaggat tgattacatg
      901 aagaaaatac gaagctatac cagtatctgg tctgttgaga aagtgttgta ctccatcaat
      961 gactttagac ttccgtttcc cataaccttt acgcaaatga catggtttgt cgtgtcactc
     1021 tttgcagtta tgatacttgg caacttgccc cctctttcta tgatagaggg agcatttctc
     1081 aaatactttg ggattcctgt ggctttcaca tggtttatgt ctacaaaaac ctttgatggt
     1141 aaaaagcctt atggattttt gaagtctgtc attgcttatg cactgcgacg aaagctgacc
     1201 tatgcaggaa aaaaagtaac gcttggcaga aaccagccac aagaagccat tacagcagtt
     1261 aggagtgaat tttatggcat atccaattaa atatattgaa aacaatctgg tctggaataa
     1321 agatggggaa tgttacgctt actatgagct tgttccctac aattactcat ttctaagtcc
     1381 ggaacagaaa atacaagtac atgattcttt cagacagctt atcgcacaaa atcgtgatgg
     1441 caagattcat gctttacaaa tcagtacaga atccagcata ctttctgcac aagaacgttc
     1501 caaaaatgaa gtcactggaa agctcaaaac ggttgcctat gacaaaatcg accaacagac
     1561 agacgcttta atatccatga ttggcgaaaa tcaagtggac taccgtttct ttatcggttt
     1621 taagttgctt ctcaacgatc aggagttttc tatgaaaagt cttaccgttg aagcaaaaaa
     1681 tgcttttact gattttgtct atgatgtgaa ccataagctg atgggcgatt ttgttagtat
     1741 gagtaatgat gaaatcctgc gttttcagaa gatggaaaag cttttagaaa ataaaatctc
     1801 ccgtcgtttc aaaatccgaa gattagataa ggatgacttc ggctatctga ttgaacacct
     1861 ttacggacag acaggcactg cctatgaaga gtatgagtat cctctatcaa agaaaaagct
     1921 ggaacatgaa acactgatta aaacctatga cctcattaag cctactcgct gtctggtgga
     1981 agaaaaacag cgatatttga aaatccagca ggaagacgaa accgcctatg tagcttactt
     2041 taccatcaac agtattgtcg gcgaactgga cttcccgtcc tctgaaatct tctactacca
     2101 gcaacagcaa tttacctttc cgattgatac gtcaatgaat gtggaaattg tagcgaatcg
     2161 taaagcccta tctactgtcc gcaataaaaa gaaagaactg aaagacttgg ataaccacgc
     2221 ttggcaaagt gataatgaaa ccagctctaa tgtggcggaa gctctggaaa gtgtgaacga
     2281 gctggaaacc aatttagacc aaagcaagga atctatgtat aagctgtcct atgttgtaag
     2341 ggtatcagca aatgaccttg acgaacttaa acgtcgttgt aatgaagtga aagatttcta
     2401 tgatgatttg agcgtgaaac tggtacgacc ttttggggat atgctgggct tacatgaaga
     2461 atttttacct gccagcaaaa gatatatgaa tgactatatt caatacgtga cctctgattt
     2521 cctcgctggt ttaggttttg gtgctactca aatgctgggt gaaaatgagg ggatttatgt
     2581 tggctacagc ttagatactg gacgcaatgt ctatctgaaa cctgctcttg ccagtcaagg
     2641 ggttaagggt tcagtaacca atgcgttagc gtctgccttt gtcggttcgc tgggtggtgg
     2701 taaatccttt gcgaataacc ttatcgtcta ttatgcagtg ctttatgggg cacaagcagt
     2761 gattgtagac ccaaaagc
//
LOCUS       DACKAC010000136         2650 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.141, whole genome shotgun sequence.
ACCESSION   DACKAC010000136 DACKAC010000000
VERSION     DACKAC010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.141"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            36..197
                     /locus_tag="I0B46_002736"
     CDS             36..197
                     /locus_tag="I0B46_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002392227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084971.1"
                     /translation="MAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGVKDVLL
                     SLFNRIVGA"
     gene            210..713
                     /locus_tag="I0B46_002737"
     CDS             210..713
                     /locus_tag="I0B46_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAR2084972.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            773..1162
                     /locus_tag="I0B46_002738"
     CDS             773..1162
                     /locus_tag="I0B46_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAR2084973.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            1149..>2650
                     /locus_tag="I0B46_002739"
     CDS             1149..>2650
                     /locus_tag="I0B46_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HAR2084974.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKG"
BASE COUNT          788 a          439 c          610 g          813 t
ORIGIN      
        1 aatggttttt aaccaatgcc caatccttag tcttactggc aattgtggtc attggtttgt
       61 ttttaggatt taagcgggag ttttccaaac tgattggctt tttggtgatt gctttgattg
      121 cggtcggatt ggtctttaat gcttctgggg tgaaggatgt cttactcagt ttgtttaacc
      181 gaattgtcgg tgcgtaaggg ggaagcacga tggaacagat gcgtgtttat attgcgaacc
      241 tcgggaaata taacgaaggt gaattggtgg gggcgtggtt taccccacct gtcgattttg
      301 atgaagtgaa agaacgaatt gggttaaatg acgaatacga ggaatatgca attcatgatt
      361 atgaacttcc ctttgagatt gatgagtata cgccaattga ggaaattaat cggttgtgtg
      421 gcttagcgga ggaacttgaa ggaacgccag ttggtgaagt tgcttctgag attcaacatg
      481 ctttcttcaa ttcttttgag gagatggtag agcatgtgga tgatattatc tattatccag
      541 attgcgatga tatgagtgat gtagcatatt tcttgattgt agaagctggt gattttggag
      601 aagtaccaga acacttgaag acgtatattg attttgatgc gtgtggtaga gacttggaga
      661 ttggaggcaa ttatttggta acgagtcgag gtgtttttga gtatccatat taacaaaatt
      721 attgaagggt agttgatttc taaactatcc ttctttttaa aggaatgatt tgatgaagaa
      781 aataaaaagc tacaccagta tttgggcagt tgaaaaggtc atttatgcaa ttaatgactt
      841 tcaactgcct tttcctgtga cttttaacca gatggcttgg tttgttttat ccttgctgtt
      901 tgtcatcgta tttgcccatg tgcctccact atctatgata gagggggcct ttttaaaata
      961 cttaggtata ccggtggcag ttacttggtt catgtcgcaa aaaacgtttg acggtaagaa
     1021 accattggga tttttacgat cttttattag ctatcactta cgatttaagg tcactttcgc
     1081 aggaaagaaa gtcaaagaac agaagaaacg atgggacgaa cccattactc tggtaaggag
     1141 tgtgaactat gtacccgatt aagtatattg aaaataatct tgtctttaat caagagggag
     1201 aatgttttgc ctactatgag ttaatacctt acaattactc ctttttgtca cctgaacaga
     1261 aatatcaagt ccacgataac tttcgtcaat tgattgcgca aaatcgtgaa ggcaagattc
     1321 atgccttaca aattgccaca gaaagtagta ttcgtgccac gcaagaacga tcaaaaaagg
     1381 aaattaccgg gcgattaaca gaagtagcga aacaacgaat tgatttacag acagaggcat
     1441 tagtatccat gattggcgat agtcagattg attatcgctt ttttattggc ttcaaactga
     1501 ttgcgacaga tgaagaggtc aatctaaaga atctcaagaa atctttcttc tccggctttc
     1561 aagaatttgt ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg
     1621 aagaaattcg ccgatatacc aagcttgaaa aactaatgga aagtaaattg gctcgaagat
     1681 ttaaagtcag acgagtcaca ccgagtgatc tgatgtattt gattgaacat atctatggcg
     1741 aaaagggtac accatttgaa gaatatgaat ttcagctacc aaagaaaaag ctgaagtcgg
     1801 aaactttggt gaaacgttac gacttattac gtcccagtcg ttgtttgatt gaagaaaagc
     1861 cccggtactt atgcatggaa catgagaatc atgaatcata tgtagcgtac ttaacaatta
     1921 atacaattgt gggggaaatg gagtttccct cctcagaact tttctattac cagcaacaac
     1981 aatttacatt cccaattgac actagtatga atgtggagat tgtgacgaac aaaaaggcac
     2041 ttgctaccgt tcgcaataag aaaaaggaat taaaagactt ggacaaccat gcctatcaat
     2101 ctgacaatga aacaaattcc aatgtcttag atgcattgga ttccgtagat gaattggaaa
     2161 ctaccttgga tcagtccaaa gaatccatgt acaaactgag ctatgtggtt cgagtaagtg
     2221 ccgaatcagt agatgaacta aagcgccggt gtgatgaagt gctagatttt tatgatgata
     2281 ccaatgtaaa actagtccgg ccttttggcg atatgatggg gctacatgaa gaattcttac
     2341 cttctagtaa gcgatacatg aatgattata tccagtatgt tacgtctgat tttctcgccg
     2401 gacttggttt tggcgccact cagatgttag gggagttgga aggcatttac tttggttata
     2461 acgtagatac ggggcgcaat gtttacctga aaccggcatt ggcctcacaa ggggtcaagg
     2521 gttcagttac caatgcgcta gcagcggcat tcctcggttc actcggtggc ggaaagtcct
     2581 ttagtaacaa tctcttagtt tattacgccg tcctttttgg tggacaagct gtgattgtcg
     2641 atccaaaagg
//
LOCUS       DACKAC010000137         2645 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.142, whole genome shotgun sequence.
ACCESSION   DACKAC010000137 DACKAC010000000
VERSION     DACKAC010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.142"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..281)
                     /locus_tag="I0B46_002740"
     CDS             complement(<1..281)
                     /locus_tag="I0B46_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084975.1"
                     /translation="MDSFQRLSLVNQFELLAKFEDQNRSYYERKIEILREGYEYHYDD
                     EIWSDLSEPFPKEDSRFVLDVLNMYRDINFSKSKLNLDENQITNSYYTH"
     gene            743..2125
                     /locus_tag="I0B46_002741"
                     /pseudo
     CDS             743..2125
                     /locus_tag="I0B46_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2361..>2645)
                     /locus_tag="I0B46_002742"
     CDS             complement(2361..>2645)
                     /locus_tag="I0B46_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAR2084976.1"
                     /translation="EVAEDDPYWEIEASYIAQCAYNTTLMFSPDVIIFGGGVMKQRHM
                     LQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGTLGCLALAREAVLHS"
BASE COUNT          937 a          473 c          453 g          782 t
ORIGIN      
        1 aaatgagtat agtatgaatt agtaatttgg ttttcatcta agtttagctt tgatttacta
       61 aaattgatat ctcgatacat gttcaataca tctaatacaa atcgagaatc ttctttcgga
      121 aatggctcag aaagatcact ccaaatttca tcatcataat gatactcgta accttctctc
      181 aatatttcga tttttctttc ataataagat ctgttttggt cctcaaattt agccaataac
      241 tcaaattgat ttactagtga caatctctga aaactgtcca ttaaatgttt cttcctatct
      301 tgttcattaa caaaaaatta taagccctta caacgaatat tatatattaa atttaattgt
      361 aggtgaacac gaattttata aatatttatg gtgttaatga caaatgatat ccgactcctc
      421 ttcatacgtt ctggactatt tgctaataga ttaaaatttc agcatctatt tctgaccata
      481 aataaatgag ctgccgaaaa gctgccaaat cagacttgaa aataaaggat caaaaacttt
      541 taagtttctg accccgtaac tcacacttca aaatcttact taaaaaggag tctgaaagaa
      601 aaaaagagta ttcaaaattt ttttctaact agctacatca catctatgca ttgacactca
      661 aagaaccaga ataagtcatc agaaagcaag ggggtgcgga cataatcctg gacgaaaaaa
      721 taaacttagg aggaatgtcc gtgtgttttc ccacaaagag cgtgtaaggg caattcagtt
      781 atttttaaaa tacgattgct cttatgctgc aaccattcga gaattagggt atccgtcgat
      841 tggtgcacta cggaaatggt ataaagagta tttggaatcc ggagagctcc atcaggagca
      901 ccgaaaaaaa tcaaagtatt ctgaagaaca aaaacgaata gcggtgaatc attattttga
      961 atatggtcaa tgttatgcac gaaccattcg catgcttggt tatcctaata gagagtcctt
     1021 gcggcagcgg tgtgaagaac ttgcaccagg agctagaaaa ctccgaaaaa gtgcggtaaa
     1081 gttaacacaa gatcaaaaag aagctgtgtt gaaaaaattc tataaaccgc aaactaatag
     1141 gaaaaattta gccgaatctg gaggaattag tagagttact ctatatcaat ggaaaaacac
     1201 ggttcttgga aaggattttc cattgtgtat gactttaaaa aatcaggaaa aacaaaaaga
     1261 gctactttta aaggaagtag aagacttaca aaaacaagta caccaattac agctcgaaaa
     1321 agcattactc gaaggagcgg ctgaactgct aaaaaaagaa aaaggcgtca atcttctttg
     1381 tctatcaaat caagaaaaaa cgatattgat tgatgcctta cgaaatcagt tcaccttgaa
     1441 agagcttctt caacagctcc agtttcctaa aagcagctat ttttatcaaa aacaagcact
     1501 tgagaaacca gataagtatt acaaagaacg tcaaattaat tatcacgatt ttcaatcata
     1561 acttctgtgc gtatggttac cgtagaattc accaagcttt aaaaaatatg ggtaaaaagc
     1621 tttctgaaaa agttgtacgt agattgatga ctgaggaaaa tctatttgtt aaattttcta
     1681 gacgaaaaaa gtattcttcg tatgctgggg aaatttctcc tgcacaccct aatttattaa
     1741 atcggaattt taaagcaaag aagccaaatg aaaaatggtt aacagatatt actgaattta
     1801 gaattctcgc agggaaaatc tacttatcac cacttgtcga ttgttacgat ggtgctattg
     1861 ttagttggac tattggaaca aagccaactt gcttcactct atgtctaaaa agggttattc
     1921 accagataat tcagcgtgcg aaggtttctt tggacgatta aaaaacgaga tgttttatca
     1981 acgtgactgg aagaatacaa caatcaatca gtttataaac aaattggatg attatattca
     2041 ttggtataat aatcaacgaa ttaagttatc tttaggaggg ctaagccctc ttcaatatcg
     2101 gaagaagcaa agatatattt gttaaaaagg tccaaaaaaa atatccgcac cccctttttt
     2161 ttgtaaaaaa ctattgcaca atacaagtag cctacggcta tcgaaatttc cataactttc
     2221 gagcaagaac ttatctcatt caaggcttga tcttccaagt aaaacaaaaa ccagttaagc
     2281 actctgccta actggttatg actaaatttt cagttgtatc tttctctacc aacactaaat
     2341 gtcgaagagc cttttttcgt ttatgaatgt aagaccgctt ctcgagccaa tgccaaacaa
     2401 ccgagcgttc cagcgttgtc ttctaatgct ggggtcacga tatatttgct taactcaggt
     2461 gtttcgacat acccattgac caattttgca aatgcgtcgt ggacgttttg cagcatatgg
     2521 cgttgtttca tcacaccacc gccaaaaatg atcacatctg gagaaaacat caaggttgta
     2581 ttgtaggcac attgagcaat ataggaggct tcgatttccc aataaggatc atcttctgca
     2641 acctc
//
LOCUS       DACKAC010000138         2644 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.143, whole genome shotgun sequence.
ACCESSION   DACKAC010000138 DACKAC010000000
VERSION     DACKAC010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.143"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            702..2561
                     /gene="ltrA"
                     /locus_tag="I0B46_002743"
     CDS             702..2561
                     /gene="ltrA"
                     /locus_tag="I0B46_002743"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAR2084977.1"
                     /translation="MNKVKTNKTLRHSEYYGMTQIFDELYVKSDKKEVFTELMTIIMS
                     DENIRLAYRNIKNNKGSYTASTDKKSIRDIKTLSEDTFLTEVKVLFRCYKPRAVRRVE
                     IPKPNGKTRPLGIPSIWDRLIQQCILQVLEPICEAKFHNYSYGFRPNRSTHHAIATCN
                     RLANRGKLHFVVDVDIKGFFDNVNHSKLLRQMWTIGIRDKWLLGIIRSMLNAPIVLPT
                     GKVEHPKKGVPQGGVLSPLLSNIILNELDWWVASQWQNTTIETEKIMDRRDKGKGIDK
                     TNQYRALRRTNLKEIYIVRYADDFKIFCRKRSHADKIFHATKMWLKERLSLEISEEKS
                     KIVNLKKGKSEYLGFELKLIKKGKKYVVESHMSKKSMTRVKIQLKKQLRKVARPKNHC
                     EQAKEIGLYNSMVIGIHQYYGIATCVNLDCSKIAYTLKPFINHKLPTKKHGKILNTFI
                     KSKYGKSKEMRWLNNVPIVPLNYVQFRLAVPLSEGTCKYTESGRSKIHSKLKLDLALL
                     LHMMRNSNYERSIEFVDNRISKYSAQKGKCAITGKFLEYEEIHCHHIVPVKQQGTDKY
                     SNLIIIHKNVHALVHAIEEKIIHKYLNVLNLTNEQIEKLNELRVKAGNSVLTV"
BASE COUNT         1022 a          421 c          506 g          695 t
ORIGIN      
        1 gcacttaaaa gaagaaaaca taagtttcct tttaaggaga actgatattt tgagcagtgg
       61 aatgatgggg taacgccctg aaacgctccc ttaatactcc gactggctgt ttaacatata
      121 gctgttagat ggtcagaagc tcggtgaagt cggctgaaag ttaccgtaaa tatgataaat
      181 tcatatacga aagttgttca gaggtgaatg atgtaaccta taggtcggag gtctataaaa
      241 tactcacggt gagaatgtaa gtaatactac tgacgaactt gcgaatgaaa gaatctatac
      301 acggactgta tagaaatgta caggattcta ccgtatcaac ggaacagaat gtatgttgtg
      361 gtttagtaag attattggtt atgaacaacc atgcaatgtt acaggcattg gaaatataca
      421 ggttcaaagc aagcacctaa aagtatatat aaagataaaa atatcgaaac atgggaagct
      481 ggcaatgtgg aggaaactat attccattga aacatttgtg acgaagaata ataagcacat
      541 ttatgccagt gaaagtggtg gcacagtacc aatgaggata tagaaatata tcggaggaat
      601 agccactagt caaaagaaat cgttaattaa atgatgtatt tcatagaatc gagtaagaca
      661 aaataaacac tacaacctaa gtaaacaaag gtagtggtgc tatgaataaa gtaaaaacaa
      721 ataaaacact tcgtcatagt gaatactatg gcatgacaca aatatttgat gaactgtatg
      781 taaaaagtga taaaaaagaa gtttttacag agctcatgac aattattatg agtgatgaaa
      841 atattcgact agcatacaga aatatcaaga ataacaaggg cagttataca gcgagtaccg
      901 ataagaaaag tattcgagac attaaaacac tgtcagaaga tacctttcta actgaggtta
      961 aagttctttt tcgctgttac aaaccacgag cagttagacg tgtagaaatt cccaaaccaa
     1021 atggaaaaac acgccctctg ggcattccat caatctggga taggttaata caacagtgta
     1081 ttttacaagt cttagaaccg atatgcgaag ctaaatttca caattatagt tatggatttc
     1141 gaccaaaccg ttccacacat catgcgattg ctacctgcaa tcgactggca aataggggga
     1201 agctacactt tgtagtagat gttgatatta aaggattctt tgataatgtc aatcactcta
     1261 aacttttgcg tcaaatgtgg acgattggaa tccgtgataa atggctatta ggtattattc
     1321 gatccatgct aaatgctcct atagtcctac caaccggtaa agtcgaacac cctaaaaaag
     1381 gtgtacctca aggtggtgtc ctatcccctt tactatctaa tatcatattg aatgaattag
     1441 attggtgggt tgcttcacaa tggcaaaata ccacgattga gacagaaaag attatggata
     1501 ggcgagataa aggcaaaggc attgataaga ctaatcagta tcgggcatta agacgaacaa
     1561 acttaaaaga aatatacatt gttcggtatg ctgacgactt caaaatcttt tgtagaaaac
     1621 gaagtcatgc agataaaata tttcacgcaa cgaaaatgtg gcttaaagaa cggttatcac
     1681 ttgaaattag cgaagaaaaa tccaaaattg tcaatctcaa aaaaggtaaa tcagaatatc
     1741 taggatttga attaaagcta atcaagaaag gtaaaaaata cgtagtcgaa agtcatatga
     1801 gcaaaaaatc aatgacaaga gttaaaatcc aattaaagaa acaattacgt aaagttgcca
     1861 gaccgaagaa tcattgcgaa caagcaaaag aaattggatt atataattct atggtgattg
     1921 gaatccatca atattatgga attgcaacct gtgtaaatct cgattgcagt aaaatagcct
     1981 atacactaaa accgttcatc aatcacaaac tgccgacaaa gaaacatgga aagatattaa
     2041 atacatttat caaatcgaag tatggaaaaa gtaaagaaat gcgatggttg aataatgtgc
     2101 caattgtacc gctcaactat gttcagttta gacttgctgt ccccctatca gagggaactt
     2161 gcaagtacac tgaaagtgga cgaagcaaga tacacagtaa attaaaatta gacctagccc
     2221 ttttacttca catgatgaga aatagtaatt atgaaagaag tattgaattt gtggataaca
     2281 gaatatctaa atattctgct caaaagggaa aatgtgctat cacgggtaaa tttctcgaat
     2341 acgaagaaat ccattgccac catatcgtac cagtaaaaca acagggtaca gacaaataca
     2401 gcaacctcat tattattcac aaaaatgttc acgcattagt ccatgctatc gaagaaaaaa
     2461 tcatacacaa atacctgaat gtattaaatc taaccaatga acaaatagaa aaactaaatg
     2521 aattacgtgt caaagctggt aatagtgttt taacagttta acttgaaaaa tatatcatac
     2581 ttaacattct tttgatggaa cgccgtatga agtgaaagtt tcatgtacgg tgtgaagcgg
     2641 ggga
//
LOCUS       DACKAC010000139         2625 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.144, whole genome shotgun sequence.
ACCESSION   DACKAC010000139 DACKAC010000000
VERSION     DACKAC010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.144"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            724..2550
                     /gene="ltrA"
                     /locus_tag="I0B46_002744"
     CDS             724..2550
                     /gene="ltrA"
                     /locus_tag="I0B46_002744"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAR2084978.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
BASE COUNT          943 a          458 c          581 g          643 t
ORIGIN      
        1 aaaagagtag gcacagcact tcattaaatc aatatttaat gtgtgttgga gaactaaaca
       61 cacgaatagt cgaatgacgg ggtaacgccc tgaaaggcta ctttaatact ctgactggca
      121 gaaacttgag taaacaaggc tctgtcagaa gctcggtgaa gtcggcagac agacaccgta
      181 acagataatg ctgacgaaag attcccagag gtgggcgttg tgtcctatat gccggaagtc
      241 tataaaatat ctatggttag aatgtagatg agaggtctac tgacgaatcc ttgaatgtac
      301 aggtctatat cggggctgga tagaaatgtc cagtgacaca aacagtgtcg ctactgtggg
      361 tgagtaagac tccgcattta tgaaaatcca tataacgtta aaggcgttat caaggtagca
      421 ggcttataga ggaaacctaa ggatatatgt acagatagtg tgtttggaac gttgaaagct
      481 gacaacatgg agattttact gtctgtgaag tgttcacatc agaaagttat tgtgagatta
      541 gcaatgatat aaggtgtgtt aatgtcagtg agagtggtgg cacagtacct atgaaaccgt
      601 gataacaagc ggtggaggga tagccactag tcatatgaag aatagaatta ctaagaaaaa
      661 tggtatgggt tcgagtaaga ctaagagatg tacttgctcc tgcaaacaag gagggtacag
      721 accatgctaa agaaaaacaa gctccgctat aacgagtatt atgatatgca acatatttat
      781 gacgagttat acgcacagag caagaacggt aataactttt ataagttact tgaaattatt
      841 ggttcggaac aaaatatttg tttagcttac cgtaatctga aatcaaacag tggcagtaaa
      901 acggctggaa cggatggaat gacgatagac gatataaaac aactcagcaa tgctgagatt
      961 gttgctaccg ttcgggaaag ccttagcaac taccgcccca aatcagtcag acgtgttttc
     1021 attccaaaag ctgggagcga taaaatgcgt ccattgggga ttccgtgtat ctgggacagg
     1081 ctggtacaac aatgtatcct gcaagtccta gagccgatat gcgaaccaaa gttccataat
     1141 cattcctatg gattccgtgc gaatcgaagt gcccaccatg cagtcagcag agtaacgaca
     1201 ctgataaatt tatcaaaata tcattactgt gttgacgtag acattaaagg atttttcgat
     1261 aatgtcaatc atggaaaact attaaaacaa atctggacac ttggtattcg ggataaaaga
     1321 ttgatttgca ttatcagcaa aatgctgaaa gccgaaattg acggcgaggg tgtcccagaa
     1381 aaaggaacgc cacaaggcgg tctgttaagt ccactgctct ccctcatagt ccttaatgaa
     1441 ttagactggt gggtaagcag tcagtgggaa acattccagc cgaagaaccg cagtaaaaat
     1501 gggtggcttc aatacgcaaa gaaatatacc aaactgaaaa gcggttttat tgtacgttac
     1561 gctgatgatt ttaaaatcat gtgttcaacc tatggagaag cacaaagatt ttaccacagt
     1621 accgtagatt ttcttaataa aaggctgaaa ttagaaatca gtccagagaa atcaaaggta
     1681 gtaaatctga agaaaaattc atcagatttt ctggggttca aaatcaaagt cattccaaaa
     1741 ggaagaacga aacacggtta tgttgccaaa acagatatga accaaaaagc attaaagaaa
     1801 gcgaaaacaa atctaaagtt aaaggtaaaa gatatagcca gacatacgac tggcttcaat
     1861 atcagcagat ataatctgac agttatcggt atgcagaatt actactgcat agcaactaac
     1921 gtatataaca acctgacaga agtaagttac gctctcttac cgaccatacg aatccgttta
     1981 aggaacattg caaaatcagt tccatttgaa agtacaagtt cagaatttca gagcaggaca
     2041 aaaggaatca gaccaaaaac aaaaatagtg atgattgcgg ataatccgct attgccgatt
     2101 caaggagtac aacacaaaaa tcctatgaat ttctcgcaag atatttgtaa ttttacaaag
     2161 caagggagaa acaaggtgca tgaggacgtt gtagttgtaa caaaagaaga aatccgagcg
     2221 ttgttggaaa atgaaaaccc tgccgatagc gtagagttta acgacaatcg tatttcggca
     2281 tatatcgcac aacaaggcaa ctgctatgta atgaatcgtc gaggaactcc gtccactctg
     2341 atatgtattc acaaatcaga caaaggagat aaccttgaca gatattctaa tctggcattt
     2401 gtggaaattc ccattgcaaa ggcaatccta acagaatcag cagacgaagc aaaatcactg
     2461 ttaaaaggtt atgttcttaa tagtcagcag aagaaaaaac tgaacagaat acggacgaat
     2521 tacggatacc agacaataac tgggaaataa cttataaatc tatttggata tgatggaacg
     2581 ccgtatgaag cgaaagtttc atgtacggtg tgaagcgggg gaaaa
//
LOCUS       DACKAC010000140         2414 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.145, whole genome shotgun sequence.
ACCESSION   DACKAC010000140 DACKAC010000000
VERSION     DACKAC010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.145"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..488)
                     /locus_tag="I0B46_002745"
     CDS             complement(<1..488)
                     /locus_tag="I0B46_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084979.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGG"
     gene            complement(498..1658)
                     /locus_tag="I0B46_002746"
     CDS             complement(498..1658)
                     /locus_tag="I0B46_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAR2084980.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            complement(1658..2344)
                     /locus_tag="I0B46_002747"
     CDS             complement(1658..2344)
                     /locus_tag="I0B46_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAR2084981.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
BASE COUNT          679 a          519 c          358 g          858 t
ORIGIN      
        1 cctcctgaat tagtgtttat agtttccaaa gattctactg taacatttac aatgtttgcc
       61 atatctttta gaatattttc tccgttttca cagcttctaa tataatcaag acttagataa
      121 ctacagcagt cgctacaata ccaaacatca tctttagaag gcaatttgcg caaattaatt
      181 cttttattga aaatgacatc cgaatcgata tagaaatatc ggtcgtcctc acgcgaatga
      241 tcttcttcta aatatttcca ccataaatat ggtttaatcg aaggaatata ctctttgtcg
      301 tcccgcagat catcgtacac atgaacttca acaccatatt ccttctcaaa aaaaatagga
      361 atctgatcat cgtgtctgct gaaaagcaat acgatatctt tgattcctag tttcttcaga
      421 ttagttaaac aaacttcaag ctcccattta aaccgattga ttgccggctg acaaagaata
      481 tacttcattc tgatcaccta cgcttgtgtt gtagttgttg tggttgttgg ttttgtagtt
      541 gtagtagtag ttcccaaagc gctaatatct aatacaatga aactatcgtt acgtttaggt
      601 tgaccgtttg catattgttt agctagataa atgcgttcgt cttcaacaaa atggtattca
      661 tctgaagctt caatttttag tgtagatcct acacccatga agtaatctga ggctacccca
      721 ataactgctt ttccttctgg cacagccgtt gactgcaaat ctgaaactgg tactggcaat
      781 acttgtacgt attctccatt agcagttagt acagtcttag ctgggaatac tttagaccag
      841 taatcagttg gattcacaat taggaccaca tcagaaggat tcacattacg ataaatcgga
      901 tcattcacac cttcgatatt gaattttgat agtcgcgcca tcaaaccgcc catagttaca
      961 gcatctaaag ctgtaatagg ttctgctttt ttttcagcat attctccgct agtttgtttg
     1021 ctcatgtcac gcatcattcc gactggcata tctttaccag taccatcaac aattgcttgt
     1081 tctaatgcaa ttctcaatga ttctactaaa acagtacgga cataacgatc taaccatact
     1141 ggacctaaat caagcattgc cttacataca ggaatataac ctgatagctt gaactgcttc
     1201 atgttaatta catcaaagcc attatctaaa acttttttaa cagcttcgca aagtttaccc
     1261 caccatgctg gattgactcc acgtgacaca atccattctg ttacaccagt tgtgttaaca
     1321 aaagtaattt tttgcaatag tggatgagat tgttctaaat cttcaaatac acgttcaaat
     1381 acagtagctg gcactaattc ttcgacccct gcaaaacctt cgtttttcac tacttcgtta
     1441 tagaattttg tttcttgtgt agttaataca cgctgaccac ggttcattaa tactaattga
     1501 tcttgatttt ttgctgttgc ttcttctaaa attttatcct gaatttcctt agataagctt
     1561 accatagctg cgctaaaaga ttcttcgtta ccatctttaa aagctttcat caattggtcg
     1621 cttgcagctg ttacaccttt taaattttta actgtcatta ttttacatct ccttgtccaa
     1681 atgttttatt taatgctgct gtaaatgcag caattttttc tgctcttttt tctttaacgt
     1741 cattcaaaat ttcttcaacg ctttgttctt ttttagcttc agtacctgag ctattttctg
     1801 catcgataat ttcatcgacc aatccataac tcaaagctgt ttctgcatcc ataaacgatt
     1861 ctttttcaag aagttcttgc aatgcttcat ctgtgccatt gaatcgtgtt ttatatgaag
     1921 cctttaccga tttatcaatt gattccagtt ggtcagcaat cgtacggaag tcatcgacat
     1981 ttccttctcc gtatgtggaa gcgcggtgaa tcatcaattg tgcattgttg tagattttta
     2041 tagtatcgcc agccattgcg ataattgaag cagcactagc ggctaagccg ttaatcacaa
     2101 cgttaacttt tgctttattt gacttaagta agttcccaat agcaatccct tgaaatacgt
     2161 ctccaccgtt tgaattaatt actacttcaa tttcttcttg atcacctaga ctatccaaaa
     2221 tatttttgat tcccttgtca gtattccctt caaagaacca actagaacca ataaatccct
     2281 gaataaaaat ttgcggtact gcgccttcat tctttactgc tagaaatgtt ttcattgtcg
     2341 tcattcgcct cacctccttt cgatacttgt tgattgtttt tagttataaa tatttcatct
     2401 gccatcgcct tatc
//
LOCUS       DACKAC010000141         2397 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.146, whole genome shotgun sequence.
ACCESSION   DACKAC010000141 DACKAC010000000
VERSION     DACKAC010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.146"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            206..2068
                     /locus_tag="I0B46_002748"
     CDS             206..2068
                     /locus_tag="I0B46_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAR2084982.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2068..>2397
                     /locus_tag="I0B46_002749"
     CDS             2068..>2397
                     /locus_tag="I0B46_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAR2084983.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          864 a          353 c          466 g          714 t
ORIGIN      
        1 aaaatttgaa aaagcgtttg attatttata ttacgactta gtgcaataaa aaacgctttt
       61 taacagtaca tgtagtcgtc tttataatag ataaactctg taatattagt taaaaagcta
      121 taagttgcac ttagttaagt gcaaacattt catattgtcg ttagtttaga aaacgcttat
      181 attagcaatg taaggagaga tgataatgca gcagctaaca aaaagagaaa ggcagctttt
      241 gatttacttg gtggaaatgt ctgaaccggt aacaatcaaa aagttggctg aaaaagaaaa
      301 tgttagtgtt cggacgataa aatacgatct agatgatata agagagtggc tgaatgagcg
      361 gaagcaagtc ctatatagca aacgtagtca gggaatttgg atgaatatta gtagctctga
      421 tcgaatcaaa ttaaaaagcg aattgatgga tgttaatcgt tttgaaatat ttgcagatca
      481 aagtctccga atcaaacgtt tactaattat gcttttagta acaaaagaag ctattactgg
      541 aatgaaaatg gccaacgcat tagatgtcag caaagatacg atcatgaatg atttagatgt
      601 gctggaaaat aattttctta aggaaggatt gaccttgaat cgtcaggcgc gaaagggttt
      661 ttggatcata ggagaagaac gtctagttcg attaacgatt gaagagattc ttcaaaaaga
      721 gttcacggat tatgacattt ataaattaat gtcattgtta ttgaatggcg gtgagactga
      781 atactttgaa atgtattctg ctactgctac acctatccaa gaggtattta atcaagtaat
      841 cattcgaatg cgacacttac tggaatttga aaatctagag aaactgaatt acgcggaatt
      901 actaaatatt ctgattcgag tgacaattgc taccgttcgc ttacgcaaag aggctacgat
      961 tggtcgttat caattggttg ctgaacaaga attagacaaa aacgatcttt cttatcgcgt
     1021 catgcagcag gtctttgatt attatcagtt gccactgttc gaggatgaat atcattatat
     1081 ctatagtgat acattcgaca cgattcacca gcaagacgtc atgaaactag ttgagaatgt
     1141 gatttataaa gtaagtaaag caatcaacca ttctttttat caagatccac aattgttgac
     1201 gaatctgtat gctcatttat ccatgcgatt atcgagaaag aaaaaatttg tcaatgaata
     1261 taatcctttt aaagaagata tcaagcagaa atacccggaa ttatttgatg cgatcaagca
     1321 agcagttcga caagaaataa gtgaacaaaa tattctgatc aatgattctt tcgtggcata
     1381 tattgcctta cactttttgg tttcctacga aagagaagaa gatctacgaa gcgttagagc
     1441 cgtctacatt tgttcaacag ggttgggtgt gactagtctg atcaagcaaa agatcaatga
     1501 agaattaaat aatatcacta tcgtggcatt tgcttcagta ttgaatgcgc aagaaatgat
     1561 tgctaccaaa gatcctgatt tggtgattag tatttttcca attaatggca tcgatcgtcc
     1621 tttcatcaaa gtccatccat tacccactga acaggacttg aaaaaaattc aacaaataat
     1681 aaaaaaaata cttgcttctt ccaatcagca ggaaaggcat aacttgaatc ttagagaaac
     1741 gtccatcaat aaagatactt tagaagactt tagcaaggaa ttgattttga aagcttatac
     1801 gatttatgaa cgtttacttg tcattttgga agcagaattg atccaagaat atcgagaagc
     1861 ttttttgctt catgtattct taatggttca tcgtatcaca tttgatcaac aatatatggc
     1921 agaaggtcgt caagtagacc agattatttt agacaaagga acactggtag gaaaaatcga
     1981 agcagttttt gcagaaaata atttaggaat caatcagtca gaaatcagtg cattattctc
     2041 atatataaaa gaaggagcgt caagttagtg aaaatgtcag acgaggcttt aaaaattatc
     2101 gaggaaagtc aaaatcggga aaagttaaaa caagtgattg atctaattga agagcgatta
     2161 gtagaacatg ctattgtacc aaccgagttg cagtggacca ttctaattaa tcacttgaat
     2221 gagatgctga aacgttctca aagaaaggaa acgattcccg tagtcgatcg ggatttgttc
     2281 aaagaagtat ccaaagaagc cataactatt tctgatgaaa tagttagaag tatcggtaat
     2341 ttaaccgatg atgaaatata tgttttgtcg attcattttg aaacagcaaa aaataat
//
LOCUS       DACKAC010000142         2349 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.147, whole genome shotgun sequence.
ACCESSION   DACKAC010000142 DACKAC010000000
VERSION     DACKAC010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.147"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            13..251
                     /locus_tag="I0B46_002750"
                     /pseudo
     CDS             13..251
                     /locus_tag="I0B46_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209247.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            253..1551
                     /locus_tag="I0B46_002751"
     CDS             253..1551
                     /locus_tag="I0B46_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAR2084984.1"
                     /translation="MKHTHKIILAVTSVCTVIILGVSIFFVTQAATNNKLENTSQTTQ
                     HSSSSSKPVEDKQTTKQLDQAKQLAASYHYDEAIALLEKDDAKEAQQLLATLKKEKES
                     LVKWEDPTKISHVFFHSLIVDPAKAFHTQQAQGYKDYMVTISEFNKTIDQLYKNNYVL
                     VNLNGLVKKGTDGKLTFTGVSLPEGKKPLILSQDDVSYYEYMDNSGFPSKLIVDKQNQ
                     IKNIYIDNKKETVGDYDMVPLIDSFIKKHPDFSYQGAKGTLALTGYNGVLGYRTSKSE
                     YGDNEKTHKEIEAAKKVADQLKEDGWSFASHTWGHLNMTQASLADIQQDNERWQNEVA
                     PILGKTNILIYPFGADISDWQPYSEANQKFAYLKQQGFDIFCNVDASTPAWGQLGTDY
                     YRNARINIDGIRFEADLKGENPILDHFINVKEMYDQKDRG"
     gene            2198..2349
                     /locus_tag="I0B46_002752"
                     /pseudo
     CDS             2198..2349
                     /locus_tag="I0B46_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
BASE COUNT          836 a          400 c          429 g          684 t
ORIGIN      
        1 ttttagtttt tcttggaaat aaaaacagcg aaaatccgaa cattttcacc tctctcactt
       61 tataactttt acaaaatagt acttctcata aaaagcgctc tagttaaaga aaataagaga
      121 aaaatcaaaa aaaacaaaag tttcttttac acttgtttct atattcgtta caatggttat
      181 gggaagtttt taaaaaggtt aagaaaagct tacgggggtt tttgaaagca aaaagtagta
      241 ggggacgata aaatgaaaca tacacacaaa atcatattgg ctgtcacttc tgtatgcact
      301 gttattattc taggtgtaag tatctttttt gttacacaag cagcaacaaa caataaatta
      361 gaaaacacta gtcaaacaac tcaacactca agcagcagct caaaacctgt tgaagataaa
      421 caaacgacga aacagcttga tcaagcaaaa cagcttgctg caagttatca ttatgatgaa
      481 gccatcgcct tacttgaaaa agacgacgcc aaagaagcgc aacaattgtt agcaactttg
      541 aaaaaagaaa aagaatcctt ggtaaaatgg gaagacccga ccaaaatctc acacgtcttc
      601 tttcatagtc tgatcgttga tccagctaag gcttttcata cccagcaagc acaagggtat
      661 aaagattata tggtcactat ttccgaattc aataaaacca ttgatcagtt atataaaaat
      721 aactatgttc ttgttaatct aaatggatta gtcaaaaaag gaacagatgg aaaactgacc
      781 tttacaggtg tttctcttcc agaaggaaag aaaccgttaa ttctttcgca agacgatgtc
      841 agctattatg aatacatgga caattcagga tttccaagta agttgattgt cgataaacag
      901 aaccaaatca agaatattta tatcgacaat aagaaggaaa ctgttggtga ctatgatatg
      961 gttccattga tcgattcatt cattaaaaaa catcccgatt tttcttatca aggcgccaaa
     1021 ggaacattag ctttaacagg ctataatggt gtattaggtt accgtacatc aaaatcagaa
     1081 tatggtgata acgaaaaaac acacaaagaa atcgaagcag ccaaaaaagt tgctgatcaa
     1141 ttaaaggaag atggttggag ttttgcttct catacttggg gacacttgaa tatgacacaa
     1201 gcttctttag ccgatatcca acaagataat gaacgttggc aaaatgaagt tgcacctatt
     1261 ttaggcaaaa caaatatttt gatctaccca tttggcgctg acatcagcga ttggcaacct
     1321 tattctgaag caaatcaaaa atttgcttac ttgaaacaac aaggatttga tatcttctgc
     1381 aatgtcgatg cctcaactcc tgcatgggga caactaggaa ctgactacta ccgtaacgcc
     1441 agaatcaata ttgacggtat ccgtttcgaa gctgacctaa aaggagaaaa tccaatactt
     1501 gatcacttta tcaacgtcaa agaaatgtat gaccaaaaag atcgaggata gaggttgtga
     1561 aagaagcgtc cagctccgag caaataagaa ctagatttct aaaatagttt ctcatatttt
     1621 ggaaatctag cgcttattgc tgaggagttg cttcttgaac accgtgaata gaggttgtaa
     1681 aaagagcgtt ttgacttatt gttgaaggtc agctctttga acaccgcgtg ttaggttgtg
     1741 aaaaggctgg tctgcatcaa gcaataagaa tgaacagaga aaaacagctt cttgaatacc
     1801 gcagataggt tgtaaaaaaa ccgttttgac ctgacgataa ccagtaaaac agaggtgaga
     1861 ccattgctaa aagtagcagt ggtctcatct ctttttttgc acaagtcata tttgattgtt
     1921 tcgttatttt aatcaggata aagttctttt tttcatgcta aagagtgatt ctttcttctc
     1981 gattttcact ttcttcgttt aacttttggt aaatacttct gtcagaaaaa gctctaggta
     2041 gacacacgaa agcagataaa aagcctaact tttgcaacag tttatgctgt cgttcaaagt
     2101 taggctttta cagtttataa atagggttct gttgcaaagt tttccaaaaa atctatttta
     2161 gtgtaaaatt gagaaaaaag acagagagga cagagtaatg aatcatttta aaggcaaaca
     2221 attcaaaaaa gacgtcatta ttgtcgctgt tggttactac ctgcgttaca atctaagcta
     2281 tcgttaagtt caggaattgt tatatgatcg tggaataaat gtttgtcata ctacgattta
     2341 tcgttgggt
//
LOCUS       DACKAC010000143         2340 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.148, whole genome shotgun sequence.
ACCESSION   DACKAC010000143 DACKAC010000000
VERSION     DACKAC010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.148"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(365..1657)
                     /locus_tag="I0B46_002753"
     CDS             complement(365..1657)
                     /locus_tag="I0B46_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAR2084985.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
     gene            complement(1931..2191)
                     /locus_tag="I0B46_002754"
     CDS             complement(1931..2191)
                     /locus_tag="I0B46_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084986.1"
                     /translation="MKKIDLINMIGMLIGILVNIVIFTDWLGVLFSNLIPILIIGICG
                     IILSILELFESRNTMNRIFACIILIVNLLPMVYFTFLYFALG"
BASE COUNT          781 a          441 c          355 g          763 t
ORIGIN      
        1 tatgggctac tctgtgatac ttattagcca ttaaactatt ttacccacat agcatatatt
       61 ttgaaatcgt tcattccgta aattcaacta aatatgccta aaaaaataaa aaatctaaga
      121 tagaataaga tttattcagt cttagatgga tttatagcaa ctttttcgta acaactttaa
      181 aaaatacgaa aagaaagagc tgttttactc tgactacgtt gctaaattgt cgtttaatca
      241 ttcatttaaa ccaaatcttt ttttatagaa ttcgttttaa tcctcctgaa tccaaaaact
      301 atgaaaaccg taattaaata gttaagagtt tttaaccacc ataattctct cggctatttt
      361 gaaatcagat aaattttgat cggacacata gttgaataaa cctaccgttg ttacggaagg
      421 taaatcgcat acttttcatt ctagaggaga aggtctcgtt gatcattaac tgattaagca
      481 ccatagccca gttttgaatt cgaccacctg accacttcgc gtgtaattct ttcgtgcgta
      541 aataaagtag ttttaggagt gcattctcat tagagaacgc tccttttttt gtgacttttc
      601 tgaagctgga gtggacactt tcaaccgcat tggtagtgta cataattttt cgaatggcac
      661 taccataatc aaatagttgt tcaacatgtg caaagttccg tttccagaca tctacagcac
      721 cagaataatg agaccaccga ttttgaaaac tgtcaaaagc agcatgtgca gcgtttagag
      781 aagaagcacc gtagaacttt ttcatatctc ggcagacttc cttatagtcc ttacttggaa
      841 tatagcgcaa tgcatttcga acaagatgaa caatacagcg ctgaacaatt accgatggaa
      901 agatcgcttt tgcgccttct tcgaggccag aaacaccgtc catcgaaatg aaaaagacat
      961 cttcgacacc acgtgctttc agttcatcaa atacttgcat ccagcgattt ttagattctg
     1021 tttgatttaa ccataatcct aaaatctcct tatttccttt gagatcatag ccaagaatgg
     1081 tgtatacagc atattctttg gcttcataat tttctcgtaa agtaacatac atacaatcaa
     1141 cgaatagaaa ggcataacac tttgctaggg ggcgggcttg ccattcttcc aattcaggaa
     1201 ggacagcgtc agtgatatct gaaatcattt catgggaaat atcaaagcca taaatagctt
     1261 cgacggttgc ggcaatatct cgttgactca ttcctcgtgc atacatggaa agaaccttcc
     1321 cttcgatgtc ggagacatct cgttttctct taggaattaa ctctggttca aaggaagctt
     1381 cccggtctct aggaacatca atagctactt caccaaaact ggttttaagc gttttagttc
     1441 catagccatt tcgacggtta tcgtgttcct taggctcttt agaatgggca tcataaccta
     1501 aatgattatt caattctcct tgaagcattt tttcaaaaag gggcccaaac acatctttca
     1561 aagcatcttg catgtcatcg acagattcag gttgataggc attcagaatg gattcagcta
     1621 acttttcggc atcaggattt cttttctttc tagccatcgt gtaatcacct cttgatctta
     1681 ttgtagaaaa agaaaaaccg cgaagcaacc acctgcctag gattaatggt tacttacacg
     1741 gtttatatta cactctcatc attaaaaaag tatctttcga tattactaaa caatgaaatt
     1801 atctatttga aatttaaatc tgataatata ttgagtgttc caatgagggc tttgatccta
     1861 ttatccatat ctttttcagt tttaatattg atacacatta taagaaaaag aaacttttgg
     1921 ttaatttatt tcatcccagt gcaaaatata aaaaagtaaa atagaccata ggcaatagat
     1981 taacaattaa tataatacaa gcaaatattc tattcattgt atttctgctt tcaaaaagtt
     2041 ctaatattga aagtataatt ccacaaatgc ctataataag tatgggaata agattagaaa
     2101 ataacacccc gagccaatct gtgaaaatta ctatatttac taatatccct attaacatac
     2161 ctatcatatt tattaaatca atttttttca ttagatattt ctcctttatt aaatctgaag
     2221 tacttatatt aaaataattc taaactattt acctcaaaaa gtgcaataaa ttatttctga
     2281 acaataaaat gatagtcaaa acaaaaaaat attgttaatc gttcagagcc atccgagagt
//
LOCUS       DACKAC010000144         2264 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.149, whole genome shotgun sequence.
ACCESSION   DACKAC010000144 DACKAC010000000
VERSION     DACKAC010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.149"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(562..765)
                     /locus_tag="I0B46_002755"
     CDS             complement(562..765)
                     /locus_tag="I0B46_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084987.1"
                     /translation="MSQLGKCYSLSKSNRKVYTLFISDSAKEGFTLGFSISFKKGTKN
                     TSISHNNRELTDSQKINGLSGRK"
     gene            complement(1224..2189)
                     /locus_tag="I0B46_002756"
     CDS             complement(1224..2189)
                     /locus_tag="I0B46_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAR2084988.1"
                     /translation="MSKRGTIINEWENLTALSNRRLSVQTLTTPLKIQWSIDRITIVG
                     KLKENIYYHTQNDVLILDFEQLMRLNENNGYVKSVGLNGWQIVDKHDENIAYIEILKY
                     QEGQGRIDFNPNKISQFLAGSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQETKRKIVPPEIKTWWRLELQ
                     LRRGKATDWYAMVHESLDSFASPHFLPTDTKHTDRIMIAGLISDQNLWAGLARHTKYK
                     YRNLLKKESQSDELVNHLRETFSESADVLKKELDTWLLGLDVTEE"
BASE COUNT          661 a          512 c          360 g          731 t
ORIGIN      
        1 attttgttcc tcctcaaaga aaaagagact tttggaaaga atttccagag ttcacgtaac
       61 cctaaaaatt catatccaaa aatctctagc ttgaagaaac ctaaaaatat tcgtataatg
      121 aacctgagtt acggagctag gtgaagtagt ttccgtaaat agtcgagagt gttagcgcac
      181 tgctcggctt ttattttgct cttttttctc gttttctttg attatagcac tcctcctaaa
      241 aaaataaaaa gacctcaggg aatttcattc cccgaacccc ttgagcctcc tcctgccgaa
      301 atggaaaggg cgcacccgct gtttatttca ttcagctatc ggctttcaca atctaacacg
      361 atcgttccgc taccagtcgc cttacaggct ccattttcgc tcctctaacg tgttttaacg
      421 attgtgccga taactaaacg aaataaacgc taaaaccgct cagaacgcaa aatatgccat
      481 tttagagcta ttgtagaaaa tgtagtttga aatgcaattt tcttctttca gctttttatt
      541 tcccacttgt aaatctgcta attattttct accagacaaa ccattgattt tttggctatc
      601 agttaactca cgattattat gagaaatgct tgtattcttt gttccctttt tgaacgaaat
      661 actaaaccct aaggtgaacc cctccttcgc gctgtcgcta ataaaaagag tgtaaacctt
      721 tctgttactc tttgacaaag agtaacattt tcctaattgt gacatggctg tcacaattcc
      781 aaacgctctc cgagggtttt cgctgtcaca agtgacaacg aaaaaaggag ccgaaagcgt
      841 gtcacgctct caactccttt tacgctttat tttgcttcac gcaaaataat cgcttatctt
      901 aaacattgac gtaaatttac tctcacttat ctgacttttt tactcggtat cactccgagt
      961 aagccgtcat attcattggg ataatctcgt ctgcttcgct ctaattttcc agagtgaggg
     1021 gtaactcctt cccccgaatt accccaaaga gtgacactgg gatatcccta aatttattat
     1081 aaaaagccaa gtctctttgc gcaactgttt tatccaagtc ggcatttgcg gattcgttag
     1141 cgttttttcg ctcttgctgc aaacgcacga cccaccatgc cgacttgtgc tgaaaataaa
     1201 aaagtggaca aaaattcatc cactcattct tccgtcacgt ccaaccccaa aagccacgta
     1261 tccaattctt ttttcaaaac atctgccgat tccgaaaatg tctctcgtaa atgattaact
     1321 aactcatcac tttggctttc ttttttcaac aaatttcgat atttatattt tgtatgacga
     1381 gccaagccag cccataaatt ttgatctgaa atcaatcctg caatcataat tctatcagta
     1441 tgctttgtat cagtcggcaa aaaatgagga cttgcgaaac tgtctaaact ttcatgaacc
     1501 atcgcatacc aatcagtagc ttttccacgc cgtaactgca attccagtcg ccaccaagtt
     1561 tttatttccg gtggtacaat tttccgtttg gtttcttgtt ccaatttttt gttgtacatc
     1621 cttatttgtc gctcactggc acgactaccc caatatgccg tttccagctt cccagaccgc
     1681 ccatagattg gtttgaatga aacagggtca accacacgat attgcgtaat aaaatcatca
     1741 ggcacatcga taatgtcgca agcaatatca gcacgtgaga aatgtggttc taaaaataaa
     1801 tcatggataa agttcttcat cgaacccgcc aaaaattggc taattttatt tgggttaaag
     1861 tcaattcgcc cttgcccttc ttgatatttc aaaatttcaa tgtaagcaat attctcatca
     1921 tgcttatcca caatttgcca accatttaga ccaacagatt taacataacc attattttca
     1981 ttcagtcgca ttaactgttc aaagtctaaa atcaatacat cattttgcgt gtgataataa
     2041 atattctctt tgagttttcc aacaatggta attcggtcaa ttgaccactg tattttaagg
     2101 ggggtggtca aagtttgcac acttaaccgc ctgttagata gggctgttaa attttcccac
     2161 tcatttatga tcgtgccacg tttactcaaa ttttggttct gttgcaaagt tttaaatcta
     2221 ctatcaaata agatagaata atagaaaaag atagcaggag gaat
//
LOCUS       DACKAC010000145         2235 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.150, whole genome shotgun sequence.
ACCESSION   DACKAC010000145 DACKAC010000000
VERSION     DACKAC010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.150"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(98..394)
                     /locus_tag="I0B46_002757"
     CDS             complement(98..394)
                     /locus_tag="I0B46_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAR2084989.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            750..1106
                     /locus_tag="I0B46_002758"
     CDS             750..1106
                     /locus_tag="I0B46_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAR2084990.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            1116..2015
                     /locus_tag="I0B46_002759"
     CDS             1116..2015
                     /locus_tag="I0B46_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAR2084991.1"
                     /translation="MGKLAIISDLHVDIKKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYEPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT          729 a          381 c          424 g          701 t
ORIGIN      
        1 acttaaagca aatcgtagtt gtcacaatct cttctaaacg atgttccatc agctgacctt
       61 cggtattagc tcaaagcact gatttcacaa cctctattta ggcatgtgtc tcagcaagcg
      121 gttgatagtt gatgatctgc atttttcgat aaaaatcggc gggaagctta ctccaccgtt
      181 ttaataattg atagaaataa ctctcttctg ctttgatttt atactcgctt aatatcaatc
      241 caaaactatt cgtttttttt gaagctaatt ttttataaat atccaatgct ttggttttat
      301 cttcattgac aaagagtgtt tttacttcaa tataagaacc ctcgatattt tgtgtttctt
      361 cgataaaata gatgtttttt atatattcct tcatagtgat tcctccaaag aagtcttttt
      421 atttgataag tttactctac cgaagagttg ttaataatcc gtgaacaaaa agtaattctt
      481 tttccaatag ttgttaaagg ttttttattt tttatagtat tttttgttat ttaagaaagg
      541 aataaggtta aacaaataac aggttgcttt ttgggtcttt ttccgttcaa gaaactgaac
      601 gaagaaaagg gaaagttgtc tcttttgtga taagagaaat cataataaac gaatgactta
      661 ctttccaata aattgagaac agtcagaacc agacaaatta gttgcatctg atgaataaag
      721 tgattattct atgacatgag gtgatacaga tgaaattaga agtgacaaaa gaagcacaag
      781 aagtattgaa gaataaatta gaagcagacg atcaactttt attagatatc gaaaacggtg
      841 acggtccatt tgcagaaagc caagtcagtt gccaattgga cacggcattt cgattgatca
      901 ttgtcaaaaa agatacacaa acagatctaa gtctttattc tgtagtggct gatacaccag
      961 ttggaccaat caaaatcaaa gacagtgcaa tactttacat ggatgatcca tccacacttg
     1021 ctttagaacc aacttataat agtttacaat taaaaggacc aagcggcttg agaaaaggca
     1081 acttgcaagt tgttcgaaaa gactagaggg ataaaatggg aaaattggcg atcatcagcg
     1141 acctacatgt ggacatcaag aaattcggcg aatcagagct gatgcagctt tggcaagttt
     1201 tgcaaaagca caagatcact cggcttcatc tagccggaga tacggctaat aaagtagacc
     1261 gttgcctagc tgttgtcgaa tttttcgtca ataaaggatt atctacgaca tttcatttcg
     1321 gcaatcacga gttggcagat gtgcccaatg aacagatgat ggaccatttt ccagacccac
     1381 attttttaaa tgaacggtat attgctttaa atgagcagac cgttctttta gggatgaatg
     1441 gctggtacga ctatcagttt tccgaattga aagattacga ggcaatcgta cgaatgaagc
     1501 gtttgtattg gtatgatcgg ttcatccagc gtgggaaaac agatctcgag gtcaatgcag
     1561 aaatgcttgc agctgcaaaa aatttgctag accagctgga agcaaaaaaa cttgaggtga
     1621 ttttatccac tcatttcgtt cctaaacggg catttatcat gtatcaatct gctccttatg
     1681 agcgctggaa caaattaaat gcatttttag gatcagaatc attcggagaa ttacttgacc
     1741 gttattcaaa tatcaaacaa gtagtttttg gccatacgca tcgtcgcttt gaagataaat
     1801 ccatcggtca cacgatttac agctgcagac cttttggtta ttattatgaa tggcagctga
     1861 cccgagattt tgtcttgaaa gaacagctgt tagatagcta tgaacccatg aaattacgtg
     1921 tcctcttgcg tgcccatcat ccggcctttc aagcctatca aacacaacat ttgcaagagg
     1981 aatttgaaca ggcgatgaca atcgttccgt attgagacag cattagtaat gtgtaaaaga
     2041 aaataccttg taaaacataa ggataagtaa gagcagtaga tccatttatc gtggatatgc
     2101 tgcttttttt gtggaaaata atcagcgaat gtttatttga tcggatgatt tgtaactcgc
     2161 agagtactag atgcaagcgt ttattctttt tttgaacgat aatcacaaaa aaaaatataa
     2221 tatacatttt ttatt
//
LOCUS       DACKAC010000146         2235 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.151, whole genome shotgun sequence.
ACCESSION   DACKAC010000146 DACKAC010000000
VERSION     DACKAC010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.151"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            74..370
                     /locus_tag="I0B46_002760"
                     /pseudo
     CDS             74..370
                     /locus_tag="I0B46_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2479 domain-containing protein"
     gene            533..979
                     /locus_tag="I0B46_002761"
     CDS             533..979
                     /locus_tag="I0B46_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084992.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            981..1118
                     /locus_tag="I0B46_002762"
     CDS             981..1118
                     /locus_tag="I0B46_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAR2084993.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            1156..1449
                     /locus_tag="I0B46_002763"
     CDS             1156..1449
                     /locus_tag="I0B46_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084994.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            1446..1670
                     /locus_tag="I0B46_002764"
     CDS             1446..1670
                     /locus_tag="I0B46_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAR2084995.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            1667..>2235
                     /locus_tag="I0B46_002765"
     CDS             1667..>2235
                     /locus_tag="I0B46_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAR2084996.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNPYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLA"
BASE COUNT          787 a          360 c          474 g          614 t
ORIGIN      
        1 gaatgctaat gcgactgcta tagatgaatt agacaataag atcgatgaac gcattaatga
       61 tacagctact accacattaa cagttacaaa cgggaatacc ggatcagcaa agctttatcg
      121 tgaaggaaaa acagtttcta tatattttgt ggctttaaac ggaaaaagca gtggtggaaa
      181 tgattcaacg atactaacaa ttccagaagg ctatcggcca ccaattagtt ttgagcaact
      241 ggttggctcg atagaccgtt ctactttgaa cagtgctcag ttatctattg gtgcagatgg
      301 agccattaaa tggcgaagaa actcaagtta tggatcggat tatacctttg caattactta
      361 cacgatttag aaaagcgtga atcgatatga aggcagcata tagaccaatt gaaccttacg
      421 gattcgagca aatcattgtg aatgatgaag aacatttacc ggaagaatgc acagaagtcg
      481 aaccaccgat tccaaattgg aaaccgaaat tcaattactg ggagggaaat aaatgaaaaa
      541 catttggaaa tatggacgta ctggcggaga gtacgcagga aaagtattgg acgacatgct
      601 tgtatccgtt ccttacacag atcagccacc gcttgaagga attcgtgctg atggcgaacc
      661 gttaacaatc gctgatcaaa tgtttgatcc taaattgaat caatggatta ttttagcgaa
      721 cgcactagat cacaacgatt taaacaatct caaagcaatg tatgagtcgt tagaaaatga
      781 gaacggcgat ttaaaacaga tcaatgccaa actcatgcta agcgatgtag caattaaaca
      841 ggaaaatact gcattgaaag aaaaagcgga tagtttagca caaatcaatt caaaaatgat
      901 gcttgcttcg ttacaaaata gcaaagacat ttcagaaatt aaagagcaac taaatccagc
      961 ttcaaaggga ggtgagtagt atgtttagtt ttagcgatgt gaaaatgatg tatgattggg
     1021 gctgtttcac taacgaacaa gtaatggttt tcgttccgtt gtgcattact gaagaaaaag
     1081 cagataaaat cattagcaaa gaagagagcg catcttaatt gatgtgcttt ttattttgat
     1141 tcaaggagtt gtcacatgat taatttaggg gaatggggag cgatagcagg atcaataacc
     1201 gctatcgttt ctttgatttt attagtaata aaaccaatta ctgcatcttt ctcgaagatt
     1261 actgagactc tttcaaaagt aagtcacaat ttagatttgc tgactaaaga tttagaatcg
     1321 agcaaatcag atcgattgat gattcatgaa gaactaaaga aacacgatga aagattagat
     1381 acacatgcag aaaaattggt agaacacacg caacaaatta aaactttatt tagggagaga
     1441 agaaaatgaa taataaaacg ttcgaagtac taaaatggtt cgcactggta attattcccg
     1501 cactagctac tttcgtgggg ttagttggta aagcgctcaa ttggcagtac acagatatct
     1561 gtgttgtcat cattactggt tttggcgcgt ttttagggag tgtgttgggt gtatcaaatc
     1621 gaacctacaa aatgttctcg gctgaaagcg aagaaggagg aaacaaatga aaaagaaaat
     1681 tactattact gcgatgagcc tgttaacggc tcttttttta ttacccatta atacgtttgc
     1741 ttatactatt aatgacgagt ataatttagc gccgaatcaa ggagactcca gattagcaat
     1801 tcctaacaaa attattttgc acgaaactgg aatagatgca ccagcaagaa acgtagccgc
     1861 caacatgaaa aataattata acggaagcaa tccttatact acagatgtta ttggtgacgg
     1921 tgggattgtt taccgtgtgg gtgagcaagg atatgtttcg tggggagctg gtaacgctaa
     1981 tccttatgcg cctgtacaga ttgaattaca gcgcacatat gataaagcat tgtttgaaaa
     2041 aaactatcga gcttatattg aatatacaag agatagtgca aaaaaatatg gaattccatt
     2101 gactcttgat caaggaactt ctttatttac aaaaggaatc atttctcatt tgtgggtgac
     2161 aaattatgtt tgggggaacc acacagatcc atatggttac ttatcgcaaa tgggagttag
     2221 caaagaaaag cttgc
//
LOCUS       DACKAC010000147         2221 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.152, whole genome shotgun sequence.
ACCESSION   DACKAC010000147 DACKAC010000000
VERSION     DACKAC010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.152"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..150
                     /locus_tag="I0B46_002766"
     CDS             <1..150
                     /locus_tag="I0B46_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAR2084997.1"
                     /translation="HAPHTIECDCGERAKCIKSYRLYQCQTCGKKYTLSYGDYILIDE
                     KGKKR"
     gene            150..401
                     /locus_tag="I0B46_002767"
     CDS             150..401
                     /locus_tag="I0B46_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084998.1"
                     /translation="MKTQHKLLTVEEVVQILVDKEVISEKVAEALLSPRHWTIEIPLV
                     EDGPTMLFEDLVKIAGYELHYQENMETYENEYWLTKLEN"
     gene            416..853
                     /locus_tag="I0B46_002768"
     CDS             416..853
                     /locus_tag="I0B46_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2084999.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLDELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFIETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            846..1553
                     /locus_tag="I0B46_002769"
     CDS             846..1553
                     /locus_tag="I0B46_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2085000.1"
                     /translation="MSNVVYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSHNRLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATIDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKRQ"
     gene            1934..2113
                     /locus_tag="I0B46_002770"
     CDS             1934..2113
                     /locus_tag="I0B46_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2085001.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
BASE COUNT          827 a          348 c          455 g          591 t
ORIGIN      
        1 catgcgccac atactatcga atgcgactgt ggagaaagag cgaaatgtat caaaagctat
       61 cgtttgtacc aatgtcaaac atgcggaaaa aaatacacct tgtcttatgg cgattatatt
      121 ttgattgatg agaaaggaaa gaaacgctga tgaaaacaca acataaatta ttgactgtgg
      181 aagaagtcgt tcaaatttta gtggacaaag aagtcatttc tgaaaaagta gcggaagctt
      241 tacttagtcc gaggcactgg acgatagaaa tccctttagt agaagatgga ccaaccatgc
      301 tttttgaaga cctagtgaag atagcaggat atgaactgca ttatcaagaa aacatggaaa
      361 cttatgaaaa cgaatattgg ttaaccaaat tagagaacta aaaaggagaa aaaatatggc
      421 tcagaattac tatgacgaat ttgtcaaatt acctttggat aaaatggcac aaaaaatgga
      481 ggatatgacg ttcctataca atgagacacg tgttccaaaa aaacattata aggaaaaatt
      541 gtcggtagct gtggaagaaa tgattgaatc aggtgtcgaa atgaatctca ttgcgacgta
      601 ctatcgaaca ctggatgaac tgaaaaagca aaatgcaaaa tggtttttcc aagccttact
      661 atgcttggaa gtaggcgtca aaccaagcac aattaaacct tctgaatacc aagctttaga
      721 actaacgtat gcgaaattta tcgaaacgaa aaaagctaag actgtttcaa gtgaatggtt
      781 agattatttt gaaaatatca ataagtatgg cgcatattac accatgaaaa aggaggacaa
      841 cgaaaatgag taatgtggtt tatcaactaa caacggaaga tgtgtcacac tttttaacaa
      901 acaaagaaca tttgaatgaa gtaatcagac ggattcaaaa agactttagt tgtatggaag
      961 aaatgaagta ctttattcaa agagtcgaag aacaattgta tttggaaagt gaaacgagtc
     1021 aaacgaaggt cacagtcagc cataatcgtt tagacgtgta taaacgtcgt cctgaaacgg
     1081 taaaaaaagg aacggagctt ctacgactag aaacagaaaa atacatcatg aatgtttacc
     1141 tagaaaggag tgagctggtc gaaaactttg tcagctacac gatttgcagg aaacactatt
     1201 actcagaatc agaatattat tcaatttttg agagcatgga attaatggaa gaagaaatgg
     1261 aagagctaaa cagcaaagat gcgacgatag acgattatta ccttgtattt actagatttt
     1321 tgacgtgtgg aacggaatct atcgtgttaa tgggaatgga ttatgaactc gctgtacccg
     1381 aagcattttt gagggaatat gtagaagaag tagataaccc aggcgacgac tggtacacga
     1441 ctttaactga ttttttagac acctatactt acgacgatac agaagaaatc caaaatgaac
     1501 ttgaccgtaa acgaatgcca tatcaacgat ttgaaaggaa aaaaaggcaa taatcattta
     1561 taccgaaaag tgagaatgtg tgaaataagg acaaaaaacc tagtaactaa gggaatggtt
     1621 cttatttcgc accaatcgct ccaaccgccc ccgccgctta ggtggtgcga aataagcggg
     1681 caacacccga taacctaaga gaaagaagga gatccatatg aataaaatca ttcctctatt
     1741 gttagtccta tttgttggcg aaatacttat gcgccatgct gcagctgtgc atttaaactt
     1801 cgtagtattt ttggcaatac tggtattttg tttgatcttt tggcgctttg tattagccct
     1861 tgtgagtcta ctattcaaga tgattgcagc catatttttc atagggttat ttcgatgaaa
     1921 ggagagagta ctaatgccaa ttgcaatagg aaataaacgg ttacccgtga cattagatga
     1981 aaagagacaa aaagaattgc agcaactaaa gcagaagtac ggaaaaagtg aatctaagat
     2041 tatgtgtatt gcgttagatc tattgatcgc gcaagaaaaa gcaggatttg atataccagc
     2101 actaaaaaag tgacgtcact tttcatttga agaaacaaaa gtgatagcac ttaattgttc
     2161 ctactattta gaatattttc tatgtccttt ttattccatt ttgtatcaat aactatgaaa
     2221 t
//
LOCUS       DACKAC010000148         2190 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.153, whole genome shotgun sequence.
ACCESSION   DACKAC010000148 DACKAC010000000
VERSION     DACKAC010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.153"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            353..1081
                     /locus_tag="I0B46_002771"
     CDS             353..1081
                     /locus_tag="I0B46_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAR2085002.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRTNESDYNKRDQVLL
                     VKLNEEISVEEAVRTFNASDMVNYAEPNYVVELEPLNDVEEPNTTDSLGNRSIILNDP
                     NWCKQANLREISMPSAWEETKGSKDVKVAVIDSGIDYQHEDLSGNVDESLGYDFFDDD
                     TDPMDTMGHGHILPGLLVLKQTTN"
     gene            1111..>2190
                     /locus_tag="I0B46_002772"
     CDS             1111..>2190
                     /locus_tag="I0B46_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HAR2085003.1"
                     /translation="MVPIRVFGSSGGTSLAILTEAINYASQKGFPIINFSIGSVVYSS
                     TEKAIKNYPGLFVAAAGNNGIDMTDKPFYPASLDIPNIITVGNSSGYERSSDSNYRKT
                     GVDLFARGFRVFSTFLGSKYTYMSGTSMATPHVTGSAALALSLDPDLSTDELKKIILN
                     SVDSFYELSDLCLTGGRLNTNNVVRQVTGKLTASNITLRLNSEWSDEIAKEMCHVHWI
                     DQSGNDLTDQVVVLKNEVDTSNFGDYEVVFASPDLTDQISVTVTVPRLRKLTLGKETA
                     TIDIGSAWDSVIAIDTFQVKAIDDFGNDVTNQVQVTINADIDTINSIQAVIKFEVPEL
                     GLAQIGRLNITAKNSTINGLNEVSIE"
BASE COUNT          769 a          289 c          470 g          662 t
ORIGIN      
        1 cgtggagaaa taataagaac taccagtcag ataaaacaag ataccgaaga ctcactgcgc
       61 aaagggtggg gaatgaataa taaagttaga taataatcaa agaagaacta agtaactacc
      121 tttaaacggt agttacttag ttcttctttg atttgcgtga aatagagggg atttattttt
      181 ctggttacgt gattaaaaaa tccaaaaaat gagcatagga acggttatga ttttagggtg
      241 tgttttaatg agatattgtg atttcattaa caagtgtatg ataatctgtt gttgagttag
      301 taaataagta atagagctaa ctctaaaata aaactattag gaggagatgt aggtgaaaaa
      361 gtttaaaaag aaaatatatt taggattggt tgttggggtg agtatgtttg ggttcatggc
      421 tcatactaaa caagtaactg cagatactaa ccaaaaagaa aggattatga gccaattaga
      481 tgactctcaa atagagtatg tacccaatga gctaattgtc agtttaaagg ctacacaaag
      541 aatttcaaca gagaaaaata aaccgcaaaa tttattgggt gtaaaagtta tggtggaaaa
      601 agatttgact cttaggacaa atgaaagcga ttataataag cgagatcaag tattattagt
      661 gaaattgaac gaagaaatta gtgtggaaga ggcagtaaga acatttaatg cttctgatat
      721 ggtcaattat gcagagccaa actatgtggt tgaactagag ccgttgaatg atgttgagga
      781 gccaaataca actgattctt taggaaatcg atcaattatt cttaacgatc ctaattggtg
      841 taaacaagct aatttaagag agattagcat gccatccgct tgggaagaaa ccaagggttc
      901 taaggatgtg aaggtagcgg tcattgattc aggaattgat tatcaacatg aagatctttc
      961 tggaaatgta gatgaaagtt tgggatacga tttttttgac gatgatacag atcctatgga
     1021 tactatggga catggacaca tattgccggg attattggtg ctcaaacaaa caacgaatta
     1081 ggtgttgctg gaataaattg ggatgttacg atggtaccta tacgtgtatt tggtagtagt
     1141 ggaggtacca gtttggcaat cttaactgaa gctattaatt atgcatccca aaaagggttt
     1201 ccaattatca attttagtat aggtagtgtt gtttatagca gtacagaaaa agcaattaaa
     1261 aactatcctg gattattcgt agcagcagct ggaaataatg ggatagatat gactgataaa
     1321 cctttttacc cagcttcttt ggatatacct aatattatta ctgtaggaaa ttctagtggt
     1381 tatgaacgaa gttctgattc aaattatagg aaaacaggtg ttgatttatt cgctagaggg
     1441 tttcgtgttt ttagtacatt tctaggaagt aaatacacgt atatgtcagg tacctcgatg
     1501 gcaacacctc atgtcactgg tagtgctgca ttagcgttgt cactagatcc tgatctatct
     1561 actgatgaat taaaaaaaat cattttaaat agtgttgatt cattttatga actatctgat
     1621 ttatgtctta cagggggaag actaaatacg aataatgtcg tacgacaagt aacgggtaaa
     1681 cttactgcat ctaatatcac attaagatta aatagtgaat ggtcagatga aattgctaaa
     1741 gagatgtgtc acgttcactg gattgaccag tcaggaaatg atttgacgga tcaagtggtt
     1801 gttctaaaaa acgaagtgga tacatctaac tttggcgatt acgaagtagt ttttgcatca
     1861 cctgatttaa cagatcaaat ctcagttaca gtgaccgtac ctcgattacg taaacttacg
     1921 ctaggaaaag aaacagcaac cattgatatt ggttccgcat gggatagtgt catagccatt
     1981 gatacttttc aagtgaaagc tatagatgat tttgggaatg atgttacaaa tcaagtacag
     2041 gttactatta atgctgatat agatacaatt aattcaattc aagcagtgat taagtttgaa
     2101 gttccagaat taggcttagc ccaaattggt agactgaata ttactgcaaa aaatagtaca
     2161 atcaatggat tgaatgaagt tagcatcgag
//
LOCUS       DACKAC010000149         2134 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.154, whole genome shotgun sequence.
ACCESSION   DACKAC010000149 DACKAC010000000
VERSION     DACKAC010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.154"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            5..>2134
                     /locus_tag="I0B46_002773"
     CDS             5..>2134
                     /locus_tag="I0B46_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2085004.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLARLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DQTGQTELQVGIQAVAKNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAENKNIVPVGSLWIDKKDGKV"
BASE COUNT          856 a          356 c          474 g          448 t
ORIGIN      
        1 ggacatggca gaagaaaaag aaccactaga caatagtcgt ttgggaaaat ccaaaagaaa
       61 attagcacga ttgcaaaatg aattaaacga acaaatcgaa aagatgtttg aacaccagag
      121 aaagaccaac ggtcagccaa tgaacgataa gagaaatggg cattcttggt ttaggcaaca
      181 agaacgttta gaaaacaaag tgcatagtct gagagaagag atcaagcaac aagaaaaaca
      241 ggtagaaaaa cttgaaagac aagaagagct aaaggaaatg ggctataaca agtacggcgg
      301 cctggatatg acaatcgaaa atattccaag aatcaaagaa gaaatcgaac gctttgaaaa
      361 aggtgaatcg actttttcag ccgctacgat ccgaaaatac caacggaaat tggaaacatt
      421 agaacagctg aaagaaagat cagaaaaagg aaaagaaaat cttcttcctg aagtacaagc
      481 aattattgac agcggacgag tgacgcaatg gaaaaaaaat ccaaccattt attttttgaa
      541 agactatcga aaagttgctt tagaactgaa tgaaaacggg ggatttgagg aatcaaaaaa
      601 ataccaagcc acgtcggaag aggaaaaagc agctgttcgt gaattacttg aagaggctgc
      661 gccaactgat tctttgaagg agaaagaaaa cgatcatttc tatcgagtag aattcaacga
      721 aaatgatcca gaaaaaggat tgaaaagtta tggtggggag atcgtaacat tagaattgat
      781 cgatcaactc aaaacgttgg ataaccaata cgccgcagaa gcaggttatt ataaattcta
      841 ttttgaagaa gtgaagaacg gagaagtagt cgatcagatc cgaatggatc taggggatgg
      901 cgtcaacagt aatcaaccca tctatttaga gttagcaaaa ttgtgcgaac aagtacctga
      961 aaaggaacaa aaacaaacgg tcaaaaatga acctgtttcg aaagaacaaa tgcagtcgaa
     1021 agaaacaaaa acaaaacaaa agggctatca aaaacgaacg gcagaagaga ttaagcaaga
     1081 agtcaaatcc ctttcgcaac aggcactgga tgcggtgaaa aagcataccc attctcctga
     1141 agacgtgaaa gaacttttga attttatgtc tcgttttcca gaacgatcct ttagaaatca
     1201 attattgatt gaggaacaat ttccaggtgc aacggcttgt ttaggtcgtg ccgcgttgaa
     1261 aaaagaagga atctatatca aaaaaggaga acaagggaac aagatctttg tcagaaaaac
     1321 ggtcaaagga ttttatgaga aaggacgtgg ctttgttcga gaaacagagg ctacaccaga
     1381 agataaaaaa cgaatcgaaa gtggtcaaat cgaagtgatc aaaaaacctt actacacgat
     1441 tgaaaaagtg tttgatatca cacaaacaca attgaaacct gaagactatc cgaaaatctt
     1501 cccgaaccgc gtctttgatt ttcaattgga tcaaacaggg cagacagaac ttcaagttgg
     1561 gattcaagcg gtagccaaaa atattggaat tcaaattcga gatatgacag aaagtgaagt
     1621 ctatcaaaga gaattaggac aagcacgtgg ggcgtacgtt cgtaacgaat ttactggcga
     1681 agaagaaatc gtcatgaata ctcgaaatac tcctacgcag catttagcca caagtattca
     1741 cgaattagcc catgcaagaa tgcacaaatt ttcagaatta gataccgcaa ctaaagaatt
     1801 gcaggcagaa atgacctcct atattgtgtg caaacatttt ggaatggaca cttcggagaa
     1861 agctattcct tatattgcag catggacgaa gaatggtcag acgttggatg ataaggaagc
     1921 ggcggaacgt gggaaaatca tgaatgatgt cagccgtgtc gcaaatgaat tcattcaaac
     1981 catttctaat gaaatcaatc aatacagaga aagagaacca gagatccaac cagcagaagt
     2041 gaagcaggag cataaaaata ctgtagaaac ggcagagaat aagaatattg tgcccgttgg
     2101 ttcactttgg atcgacaaga aagacgggaa agta
//
LOCUS       DACKAC010000150         1992 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.155, whole genome shotgun sequence.
ACCESSION   DACKAC010000150 DACKAC010000000
VERSION     DACKAC010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.155"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            272..838
                     /gene="amaP"
                     /locus_tag="I0B46_002774"
     CDS             272..838
                     /gene="amaP"
                     /locus_tag="I0B46_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAR2085005.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            851..1039
                     /locus_tag="I0B46_002775"
     CDS             851..1039
                     /locus_tag="I0B46_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAR2085006.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1052..1609
                     /locus_tag="I0B46_002776"
     CDS             1052..1609
                     /locus_tag="I0B46_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAR2085007.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1641..1883
                     /locus_tag="I0B46_002777"
     CDS             1641..1883
                     /locus_tag="I0B46_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAR2085008.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          723 a          308 c          406 g          555 t
ORIGIN      
        1 ataaatagaa tcttgtaata ataactgagc ataatctttg caatttttaa tcttgaataa
       61 tatcctttaa gtatagccag caatcaaaag taaatagaga ataagtaagc aattgttatt
      121 tcagtatcag actggcaata aaacgccgtt atgatccagc aatgtttggt agcgctttat
      181 ttactgatgc tatgctaact gttcgctatc tatactgtga ttgtcggtag agttatttac
      241 agggtaaaaa taagtttata ggaggtggaa aatgaataag gggttaaaaa taatcggtgt
      301 tatcatgagt ttactgctac tttcagtatt tctagcgatt gcgttgatca atgcgccgta
      361 tattatgcct gaacaattgg aacgatttcg cttctttact ttaacgaatt actacatgca
      421 acaatatatt ttctgggcag ctgtagtatt tgccgttctt gctattatca tcttgctagt
      481 cgttcttttc tatccaaagt caagaggaac tttcgttatg aaacgggaag atggcaaact
      541 aacaatcgac aaaaaagcga tcgaagggtt ggtacgttcc catttacacg aagaagagtt
      601 cattcattca cctaaagtac ggatacgttc aacgaaaaat cgaatccata tccatgtcaa
      661 cggagacttg aaacgaactt cttcattagt agggaaaaca ggtgtattga tgcaagatat
      721 cgaagaagaa gtaactaaag tactcggaac aaaagaaacg gtcaaagtgg cagtcacata
      781 cagcggttat gaagaacaag aagatacaag agactacaag cactctcgag tggaatagga
      841 gggccaaata atgaaagaac tattttcagc atacaagctc cctattatct ttgggggtct
      901 tggactactg ctagctgttt tatggttaac gattggattt ttcaagacat tgttgatcat
      961 aatcatgaca gcaattggcg caggaatcgg tttttatttg aaacagtcag gaatagttga
     1021 tcaatttttt aaatcatagg aggaaacaaa gatggaaaac aaaaacatga atacaacaaa
     1081 tggatcagtt ttggcaaatg aaaaaggaga aaaccgcatg atgaatggtg aattgactta
     1141 tgaagacaaa gtcatccaaa aaatcatcgg ttatgcattg aaagatgtag atggtctatt
     1201 gacattagat ggcggatttt tctcaaatat ggctgaaaaa ttagtcaaca cagataacgt
     1261 aacagcaggt atcaatacag aagtaggtaa gaaacaagta gctgtggatc tagatgtgat
     1321 cgtagaatac ggtaaagaca tcgaagatat ttacaaccaa atcaaagaat tgatcagtac
     1381 tgaagtaaac aatatgacac atttagatgt tatcgaagtg aatgtgaatg ttgcagacat
     1441 caaaacacaa gaagaatacc aaaaagatca agaaacagta caagacaaag taacagaagc
     1501 agcaaaatcc acaggacaat ttgcttcacg ccaaacagat aaagcaaaat ctgctgtagg
     1561 caaaggtgca caaaaagtca aagaaaataa cgaaccacgt gtacaataag aagtaagaag
     1621 tcagttagga ggaatttaaa atgggattta tttgggcatt aatcgtcggc ggtatcattg
     1681 gagcaattgc aggagcaatc acgaataaag gatcatcaat ggggatcatc gcaaacatca
     1741 ttgctggttt agtaggttca gcaattggac aagctttact aggaacttgg ggaccaacaa
     1801 ttgcaggaat ggctatcatt ccatcattga tcggtgcaat cattttagta ttagtcgttt
     1861 cattctttgc acgtaaatta taagcaaatt gaagaaaaac ttatgttgaa atgaagaaca
     1921 aatttgagaa ccgtagcgac actcgctgcg gttctttttt atatatttta tatgaagtat
     1981 tctttattcc tt
//
LOCUS       DACKAC010000151         1828 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.156, whole genome shotgun sequence.
ACCESSION   DACKAC010000151 DACKAC010000000
VERSION     DACKAC010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.156"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            184..1026
                     /locus_tag="I0B46_002778"
     CDS             184..1026
                     /locus_tag="I0B46_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAR2085009.1"
                     /translation="MNANSIVVGNFKGGTGKSTSTQMLGFVNAFYKKRKTLLIDLDPQ
                     ANASEVMSFTADNLGNHFYQENGFPKTIWNVLYDGSLDGAVLPLIENLDLIAGNIAMS
                     DYSDFMSAKFPNDKLAQFEYFANILAPLKEEYDEIFIDVPPSLGTIVQSAMYFAEYVA
                     IMLQTQPKSIRGAADYIDYMEFFTERYNTQLGIAGIIPFMLDTKTSTESYMYNEAKKL
                     YGDNLINTIVFRSARLIRYDETGITLETKKNGELKKVDANPQNTFISILDELNEHISW
                     FEEE"
     gene            1029..1583
                     /locus_tag="I0B46_002779"
     CDS             1029..1583
                     /locus_tag="I0B46_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2085010.1"
                     /translation="MAERQSKLFPNKKARPRTNGMKDMEEFTKSLSISSADNSAPKVN
                     TTEKHSDDNKNLKNPNKSHKGKAGRKAEYKDPRLKKDLNTKISLSTKLRIQRLISRKF
                     DNKSEGDVIDIALDYLVSSFDRDDRDSLYKAYKEDMESMIPIIQEKNEKAKKQGKLTL
                     DITDEINQQTLKEQKEKWVSAKFE"
BASE COUNT          752 a          253 c          277 g          546 t
ORIGIN      
        1 aactttgatt aaagattctt caatcaaaaa aacttaaaaa cattgatata atagcattta
       61 ataaaaataa gtatctcgta atcaaaaaaa taatttgatt acgagatact tatttacatt
      121 tacaacagca agttgtatac ttaatttgaa taaaagatac ttaatcaaaa ggagcgtaat
      181 aatatgaatg caaattcaat agtagttggt aattttaaag gaggaacagg aaaatctact
      241 tctactcaaa tgctaggctt tgtaaatgca ttttacaaaa aaagaaaaac attgcttata
      301 gatcttgatc cacaagcaaa tgcttcagaa gttatgagtt tcactgctga caacttaggg
      361 aatcattttt atcaagaaaa tgggtttcct aaaactatat ggaatgttct ttacgacggt
      421 agtttagacg gcgctgtcct tcccctaatt gaaaatttag atttaattgc cggtaatatt
      481 gctatgagtg actactctga ttttatgagc gctaaatttc caaacgataa attagctcaa
      541 tttgagtact ttgcgaacat tctagcacct ttaaaagaag aatatgacga aattttcatt
      601 gatgtaccac cttcactcgg aactattgta cagagtgcaa tgtactttgc tgaatatgta
      661 gctattatgc ttcaaacaca gccaaaatca attagagggg ctgctgatta tatagattat
      721 atggaatttt tcactgaaag atataataca caactaggta ttgctggaat tattccattt
      781 atgttagaca caaaaacaag tacagaaagc tatatgtaca atgaagctaa aaagttatat
      841 ggcgataacc ttatcaacac aatcgttttc agaagtgcta gattgattag atacgatgaa
      901 actggtatta cattagaaac aaagaaaaat ggggagctta aaaaagtaga cgcaaatcca
      961 cagaatactt tcatatctat tttagacgaa ctaaatgaac atattagttg gtttgaggag
     1021 gaataaagat ggctgaaaga caatcaaaac tatttcctaa caagaaagca cgtccaagaa
     1081 cgaatggtat gaaagatatg gaagaattta caaaatcttt atctatatct tcagctgata
     1141 attccgctcc taaagttaat acaacagaaa aacattctga tgataacaag aatctaaaaa
     1201 atccaaataa aagtcataaa ggtaaagctg gcagaaaagc tgaatacaaa gatccaagac
     1261 taaaaaagga tttgaataca aaaatctctt taagtaccaa attaagaatc caacgcctta
     1321 tttcaaggaa atttgataat aaatctgaag gtgatgtaat tgatattgct cttgattatt
     1381 tggtatcttc atttgataga gacgatagag attcattgta taaagcatac aaggaagaca
     1441 tggagtcaat gataccaatt attcaagaaa aaaatgaaaa agctaaaaaa caaggtaaac
     1501 taacactaga tatcaccgat gaaataaatc aacaaacttt aaaagaacaa aaagaaaaat
     1561 gggtatcagc taagtttgaa tagaagatac ttaaaacaac ataaactatt gattgaagta
     1621 tctggtaata ttgattttaa gatacctcaa tcaatagttt tttctttaaa ataagcattt
     1681 atgtttgaat aagagatact aaaatcaaat aaaagtatct tttaatcaac aaactagttt
     1741 gaataaagtt actcataatc aaattataaa tgatttttag atactacaat cagatagaaa
     1801 ccctattaaa ttaactttct taattgat
//
LOCUS       DACKAC010000152         1701 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.157, whole genome shotgun sequence.
ACCESSION   DACKAC010000152 DACKAC010000000
VERSION     DACKAC010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.157"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..255
                     /locus_tag="I0B46_002780"
     CDS             <1..255
                     /locus_tag="I0B46_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2085011.1"
                     /translation="NFRVALGRMSEMGYGMMFGSDVQKDFFLKRIKGRGYVDVGTSVI
                     SEFYTPLVPKGYDFLEEIKKLSNSRQSTQATCEAEVAGVD"
     gene            258..411
                     /locus_tag="I0B46_002781"
                     /pseudo
     CDS             258..411
                     /locus_tag="I0B46_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            434..1639
                     /locus_tag="I0B46_002782"
     CDS             434..1639
                     /locus_tag="I0B46_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAR2085012.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
BASE COUNT          547 a          322 c          377 g          455 t
ORIGIN      
        1 aatttcagag tggctttagg tcgtatgtct gaaatgggct atggcatgat gtttggcagt
       61 gacgtacaaa aggatttctt cttaaagcga atcaaaggtc gtggctatgt tgatgtagga
      121 acaagtgtca tatcagagtt ttatactccc cttgtaccaa aaggatatga tttcttggag
      181 gaaattaaaa agttatccaa cagcagacag tccacgcagg cgacgtgcga agcggaagtc
      241 gcaggtgtgg actgatcttg ctggctggtg tggcaatagc cacgccagca cttaacccct
      301 cggtatctaa caggggggta caaatcgaca ggaaacagtc aaaaaaacat tagaaaatcc
      361 tttggttaca agggatttac aaaatttcag cgtatgtcaa atgggcttta aaagttgaca
      421 tacgcctttt tgattggagg gatttttact gaatgaacaa acttggttac agcatttaaa
      481 agaaaaacgc ttggcttatg gactatctca aaaccgttta gctgttgcga ctggtattac
      541 aaggcagtat ctaagcgata ttgaaacagg aaaagtcaag ccatcagagg atttacagca
      601 gtccctttgg gaagctctgg aacgcttcaa tcccgacgct ccccttgaaa tgctgtttga
      661 ttatgtaaga attcgctttc cgacaacaga cgtacagcag gtggtcgaaa acatcttaca
      721 actgaaactg tcctattttc ttcatgagga ctatggtttc tattcttatt cagagcatta
      781 tgctttaggc gacatattcg tcctttgctc ccatgaactg gacaaaggag ttctggtgga
      841 attgaaaggt cgtgggtgca gacaatttga aagctatctt ctggcacaac aaagaagctg
      901 gtatgagttc tttatggacg ttttggtggc tggcggtgtg atgaaacgcc ttgaccttgc
      961 cattaacgat aagacaggga ttttaaatat ccctgtactc actgaaaagt gccaacagga
     1021 agaatgtatc tccgtcttcc gcagttttaa aagctatcgc agtggcgaac tggtacgcaa
     1081 agaggaaaag gaatgtatgg gaaacaccct ctatatcggt tcattacaaa gtgaagttta
     1141 tttctgtatc tatgaaaagg actacgagca gtacaagaaa aatgatattc ccattgaaga
     1201 cgcagaagta aaaaaccgtt ttgagattcg attgaaaaat gagcgtgcct attatgcagt
     1261 ccgtgattta ctcgtctatg acaatccaga gcataccgcc tttaaaatta tcaatcggta
     1321 tatccgtttt gtagataaag acgattccaa acctcgttct gattggaaac tgaatgaaga
     1381 atgggcttgg tttattggga acaatcgtga acgattaaaa ctaaccacaa aaccagagcc
     1441 ttactccttc caaaggacgc tgaactggct atctcatcaa gttgccccga ccttaaaggt
     1501 tgcgattaaa cttgatgaaa tcaaccagac gcaggttgta aaagacattc tcgaccatgc
     1561 gaaactgaca gaccgacaca agcagatttt gaagcaacag tcagtaaaag aacaggacgt
     1621 gataacaaca aaaaaataac tcaaatacaa attcattgaa tatagagagg agaacatttt
     1681 tatgaatttt ggacaaaacc t
//
LOCUS       DACKAC010000153         1701 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.158, whole genome shotgun sequence.
ACCESSION   DACKAC010000153 DACKAC010000000
VERSION     DACKAC010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.158"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            466..1065
                     /locus_tag="I0B46_002783"
     CDS             466..1065
                     /locus_tag="I0B46_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAR2085013.1"
                     /translation="MKTVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLADVLEISRGRVVVKDRLAMKEQKTAKHNSVYISNKLRKTILEYVQQEFPDQ
                     LLQQDFDGYLFPSRKGTNEPLNRQSLWRIIHDAGTAIGLKDIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            1129..>1701
                     /locus_tag="I0B46_002784"
     CDS             1129..>1701
                     /locus_tag="I0B46_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2085014.1"
                     /translation="MSTLYIIGNGFDLSHGLKTRYTDFSKWVEKSHSEIFDKIDTMIN
                     ENNEVRSTADDKIGWFNFESSLHELIQSPVFEDYVEESKDYLIGYGHEDWSDSAHHDF
                     QNMVYDYVVSLEELQQLFKEWIRKEIQPTIEHKIPKYQFSSDVLFFTFNYTDVLEILY
                     KQPKENIYHIHGSYDDIIIGHSENFDNFIGD"
BASE COUNT          612 a          257 c          321 g          511 t
ORIGIN      
        1 acacaaaatt attgacgctc ccaactaaaa gtgtcaaaat aatgatgaaa tcatttagtt
       61 cttataaatt agaaaagaga aaataaatat tccatatcta ctttattgga tccgtaacat
      121 gactaaattc tgaatacttt ttaaagctta tcatttggta aaactgaata taagttaatt
      181 atatagactg aaaattttga attttcagaa tagaataact taaaagtact gacaaaattt
      241 tttaaagggg caatattaat gttctgagat aatcaaatcc taccgaaaat atcaaaaatc
      301 caatgtgaca caatcagatt gtgtctcatt cgagggaatc acacaatccc acgtggctct
      361 aggcagaatt gaatttttgc ttggggcttt ttccttatat tgattttgaa tgagacttat
      421 tatatgttat aatgcaatta agtatcattg gagagggcga agaaaatgaa gacggtagaa
      481 cctattcgag acaaaaaaaa gatcgatgcg atgaaggcaa tcttggcttc gggaaaatat
      541 ggccaacgaa atttggttct tttttcgatt gggatcaaca ccgcctatcg catttctgat
      601 ttgcgtcaat tgaagctggc cgacgtgctg gaaatttcac gcggtcgcgt ggtggtcaaa
      661 gatcgattag cgatgaagga acaaaaaaca gctaaacata attcggtcta tatttccaac
      721 aaattgcgaa aaacgatttt ggagtacgtt caacaggaat ttcctgatca actccttcag
      781 caagattttg acggttattt gttccctagt cgaaaaggga cgaatgaacc gctcaaccgc
      841 caaagtctgt ggcgaatcat tcatgatgct ggcacggcaa tcggattgaa agacattggc
      901 ccacattcca tgcgaaaaac gttcggttac tttctctata aacaggggac caaaacagaa
      961 atcattcagt ccttgttgaa ccattcttct cagcgcgaaa cattgcggta tattggtatt
     1021 acacaagaag acaaagatac agctgtaaaa agcttagatt tataatataa aagttaagtt
     1081 taaacatgga tagttttaaa ttagtagatt gagtgttggg aggaaagaat gagtacatta
     1141 tatattatag gaaacggttt tgatttaagt catggtttaa aaactagata cactgatttt
     1201 tcaaaatggg ttgaaaaaag tcatagtgaa atattcgata aaatagacac aatgataaat
     1261 gaaaataatg aggtgagaag tacagctgat gataagatag gttggtttaa ttttgaaagt
     1321 agtttgcatg agttaattca aagtccagtc tttgaagatt atgtagaaga gtctaaggat
     1381 tatctcatag gttatggaca tgaagattgg tcagattctg cccatcatga ttttcaaaat
     1441 atggtttatg attatgttgt ttctctagaa gaattacaac aacttttcaa ggaatggata
     1501 cgcaaagaaa tacagccgac aatagaacat aaaattccaa aataccaatt ttcgtccgat
     1561 gtactattct ttacattcaa ttatacagat gttttggaaa ttctatacaa gcaaccgaaa
     1621 gaaaacatat atcatattca tggcagttat gacgatataa taatcggcca ctccgagaat
     1681 tttgacaact ttataggaga t
//
LOCUS       DACKAC010000154         1680 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.159, whole genome shotgun sequence.
ACCESSION   DACKAC010000154 DACKAC010000000
VERSION     DACKAC010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.159"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            197..>1680
                     /locus_tag="I0B46_002785"
     CDS             197..>1680
                     /locus_tag="I0B46_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAR2085015.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVKKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASEAEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLSKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQ"
BASE COUNT          639 a          251 c          367 g          423 t
ORIGIN      
        1 ccaaagctct tagttgaaac gtgggtcgat gtatattcga tgttgaccga cgttttttct
       61 atgccttcag atttggtttt aagcgatatc tgtttagatg acattttgca aatggcttac
      121 aacaagagtg cttatgaagg atggaaaaac tatgcaataa accaatccca gaaaaactaa
      181 agaaaggagg taaaaaatgg ctaaaaagag aacagaagca gaagtaactt tcatagctaa
      241 cgatgacgga ttgaaatcta cgttaaaaga aatcagtgct gaattaacta aaaatagagc
      301 agaattaaaa ctagaacaag ctcaattaca acagactggt tctgaatcag acaagttagg
      361 aagtaaatta tcttctttag aaaagcagta tgaattacaa agtcaaaaag ttgaagtaac
      421 tagccaacgt ttagccaatg cgaaaaaata ttatggagaa aattccaccg aagttaagaa
      481 acttgaaaga gaactgatta atcaacaaac agcgcaacaa cgtttgtcaa acgaaattga
      541 taaaacgagt aatgcactag ctcaagcaaa aggcgaaata cagacgtacg agtctacaat
      601 gcaacagttg gatagcgaac aaaaaaatgt tcaagctagt gcttctctga ttgaatccga
      661 atacaaaaaa tggcaagcaa ctgctggtca atcagcttct gaagccgaga aattagcgaa
      721 agcccaagaa tatgtttctc aacaatctga aaatgcggag aaaacgatag atatcctaag
      781 acgacagtta gaagctacac agtctgaatt tggcgctaca tccacagaag caatgcagat
      841 ggaagcgaag cttaatgatg ctgaacgtga atttgaagag ttaggacaag ctgctaaaaa
      901 tgtagataca actaacttgg acgatatcgg aagcaaaata gatatgaata atttaatgga
      961 agcttctgac gttttaagcg acattggcga taagcttaca gaattaggga aacaagcagt
     1021 ggactctgct aacagtgtag gtagttccca gagtaagata caagctaatt ttggtttgtc
     1081 taaacaagag gctgaagaat taacgaatgt agccagagac atttattata aaggttttgg
     1141 agaatcgtta gatcagtcca cagatgcatt gattttggta aagcgtaatt taggagattt
     1201 aaataatcaa gatttacaaa atatcacgga acaagctatg gtcctagaaa acaccatggg
     1261 cgctgatatg gatgaaacgt tacgtggtgt aaatggctta atggtcaatt tcggcttgag
     1321 cgctcaagat gcaatggatt taatggtttc gggtactcaa aacggtttag ataaaacgca
     1381 cgaattaggc gacaatatgg cagaatatag ccaattatgg agtcaaatgg gatattcagc
     1441 tgatgaaacg ttcggaatgc ttcaaaatgg tttagatgcg ggtgcttata accttgataa
     1501 agtcaatgac ttagttaagg aaatgggaat atcgttaaca gatggtcgat ttgagcaaaa
     1561 catggatatg tttagtgaaa gtactagaaa agcttttgaa gagtggaaaa atggcggagg
     1621 aacacaaaaa gacgttatta attccatgat tcaagatttt agcaatatgg atggtcaata
//
LOCUS       DACKAC010000155         1646 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.160, whole genome shotgun sequence.
ACCESSION   DACKAC010000155 DACKAC010000000
VERSION     DACKAC010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.160"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..582
                     /locus_tag="I0B46_002786"
     CDS             <1..582
                     /locus_tag="I0B46_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAR2085016.1"
                     /translation="LDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYD
                     LAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNYAHIDQFGEIENANLHVAGWHI
                     ANYKYEYIFIMDYNTGKELARVRADGIYRSDVNQAYNTSGNVGYHVSFNMRNFPNKKV
                     YVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
     gene            771..844
                     /locus_tag="I0B46_002787"
     tRNA            771..844
                     /locus_tag="I0B46_002787"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:805..807,aa:Ile,seq:cat)
     gene            complement(1357..>1646)
                     /locus_tag="I0B46_002788"
     CDS             complement(1357..>1646)
                     /locus_tag="I0B46_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2085017.1"
                     /translation="ETQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINLYYSNK
                     NYFKIPAAKTRMKKDVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
BASE COUNT          546 a          285 c          290 g          525 t
ORIGIN      
        1 ttggacactc cttataacca accgggaatc aaatcgcatt tatgggtaac acaaaacatc
       61 tggggcgatc atacagatcc ttacggttat ctttctgaaa tgggcgtaag taaagaaaaa
      121 ttagcatatg atttagctca tggatttacc gatgaaaatc caacaacttc tgaaaacaag
      181 cctgtcattg atccaacacg agctggtgca gctaatccta cactgacaga tggaacgaat
      241 tacgcccaca ttgatcagtt tggagaaatc gaaaatgcaa atttgcatgt agctggatgg
      301 cacattgcta actataaata cgagtatatc ttcattatgg attacaatac tgggaaagaa
      361 ttagctcgag taagagctga tggaatttat agatcagatg taaatcaagc ttataatact
      421 tctggaaatg ttggctatca tgtatctttt aacatgcgta acttccctaa taagaaagtc
      481 tatgtcatga tgcgggcaac gaatgatcca gagggaaaca ctaaaggcgg tgcgcaagat
      541 ttccatgaca aacgttggta tttaaatatt cctaaacgat aaaaatagct cctcgttgag
      601 gagcattaca taactatatt gacaactata aaaatcattc gataaaatag tgatgttatc
      661 gcatatcttc actatcaccc ataatagtca cactccaagc tatgcgataa caggtttgtt
      721 gccacacatt ctactggttg attgtttatg gctttatgtg gcaacaacca gtacccttag
      781 ctcagttggt cagagcagac ggctcataac cgtccggtcg taggttcgag tcctacaggg
      841 tacattaacg tagccatttg aatcgttctg tgttagaatt ttttgaagag tattatacaa
      901 gctaaagctt ttcttcattg ccactcaaat gagtggcttt tttatgtatc cttttatgga
      961 ttaatgaaag gatgtttcac atagttatat ttctgtatat ttgaaaagtt ttactttgat
     1021 ttttaaatag aaagacattt gggttaaatt gtgagatgat aataaagaag agtttaaagc
     1081 gcaccccaag ccacttcccc ataagtgtgt tacgctttaa actcttttat atttgaagct
     1141 attaaaaggc ataccatatt tttgaaaaaa agtgagaaaa aaggcttaca attggagtgg
     1201 tagttaatta gtgacttatt tttgatttta tagcactgat actataaaat atagatatca
     1261 tcatattaca caatcttaat actaacttaa aaaatatctc ctttcacaag tatggtgata
     1321 aaattcgttc cgggctacct ttttaggtag cctactttaa tctttgtatc tttctggatc
     1381 aacgaaagta tactttatat agtcataacg ccgatgatcg ctacgtgcgt ccggcacgtc
     1441 agtcacgata tcaaacaaaa agtatacatc cttcttcatt ctagttttcg cagcaggaat
     1501 tttgaaatag ttcttattag aatagtagag attgattaat aagctatctt cgattgctaa
     1561 aaagaaaact tctgaattcc atactttata aaaatctttg ataaatctat tcgaagggtc
     1621 aaatttaaac cataattgtg tctcat
//
LOCUS       DACKAC010000156         1624 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.161, whole genome shotgun sequence.
ACCESSION   DACKAC010000156 DACKAC010000000
VERSION     DACKAC010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.161"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            290..697
                     /locus_tag="I0B46_002789"
     CDS             290..697
                     /locus_tag="I0B46_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2085018.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            711..1112
                     /locus_tag="I0B46_002790"
     CDS             711..1112
                     /locus_tag="I0B46_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2085019.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1114..1485
                     /locus_tag="I0B46_002791"
     CDS             1114..1485
                     /locus_tag="I0B46_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2085020.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
BASE COUNT          608 a          208 c          282 g          526 t
ORIGIN      
        1 ttttgagggg aagtacatat agttgttgat atagttgcta atattgttag ctaatttgtt
       61 agtaaaactg tatacgaatt tagatagtaa aaaaacagtt tgttccttat atagaggcac
      121 ttttactact gattaaatgt cagatatgct tacaaatata cttataaata tgcttacaaa
      181 attagctaat atttccgttg acaaagcttt gttactattg ttaaactaaa ttgactagca
      241 aaagtgaaac ataaatttat taaataaatg taaaaattag gagaaatcaa tgtcttatag
      301 agtacaatta ataatttcag aagatgtaga aagtcagcaa tttggcacta atgttattaa
      361 taaagtaatt aatccagctc tttctattaa tgcgccttta attcctactg ctttatcatt
      421 ttctgtaaca gctgtggtgt ctgagataga ggatacagaa aagataaaaa tagtagaaat
      481 tgaagtttta aacaagaatg aaaaacaaat tttttctacg ggtgaagtat cggtaagctt
      541 gcctcctcaa gttaacgata taaactttaa tataaacgcc agaaatgtct tggttgaaga
      601 agcaggagag cattatgctg ttgttaaatt taatggaact gagattggaa ggcaaatctt
      661 tgatatcaag gtcaacaaac cagtggaaaa aaattaagga gacgatacgg atgcttgata
      721 tagtaccatc aaatacacat cggagtggta gcttaataaa ttttgctgcg atttttgctt
      781 gcgcatcggt aattctagca tctccagttg ttgtggatgc aagttctact ccgaaaacta
      841 aaaatgataa tcaacttgta attactacaa aaaatgagat taacacaatt tcaagtaatg
      901 atggcaaaat ttttgatgtg ataaatacgg ttttaaaaag aaaagctgaa atcaatataa
      961 aatatgatga agatttaaac ttgtatttct ttgttatcaa aacaacatct gaattgtttt
     1021 cctcagatta cgatgttttg gatactctcg ataatgtatt atctgactat aaatatatgg
     1081 ggaaatctgt agtagcaact ttgggggaat aaaatgtttg actttaatga atatttatct
     1141 atttccaaac aaattgaatc agatgataaa tataattcta aagaatctta tagaagaact
     1201 gctgtttcaa gagcatacta cagtgctttt aaaaaatctg atgagtattt gaaagaaaat
     1261 tatgatatta tttataatgg aagtagcgga aaaggaagcc accaaaccgt ttggaatttg
     1321 ttttcaacag ttaaggaatt gaatacctta ggaatacata atagtgggta tagaatgtta
     1381 gagaaaagaa aatgtgctga ctatattcct aatgaaacca ttacgaaaac agatatggcg
     1441 ctgatgaatc gagaagcaga aaaaataata aacaaactca cctaatgaat ttcaagcgag
     1501 tttgtttgtt atttttttat ttaatctttg tatctttctg gatcaacgaa agtatacttt
     1561 atatagtcat aacgccgatg atcgctacgt gcgtccggca cgtcagtcac gatatcaaac
     1621 aaaa
//
LOCUS       DACKAC010000157         1594 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.162, whole genome shotgun sequence.
ACCESSION   DACKAC010000157 DACKAC010000000
VERSION     DACKAC010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.162"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..1485
                     /locus_tag="I0B46_002792"
     CDS             <1..1485
                     /locus_tag="I0B46_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAR2085021.1"
                     /translation="CEAKFSENSYGFRPNRSVENAIKATYNRLQISQLHYVIEFDIKG
                     FFDNVNHSKLIKQIWAMGIRDKHLIFILKRILKAPIKMTNGTITYPEKGTPQGGIISP
                     LLANIVLNELDHWVESQWQENPVTKNYVVHINKSGSPCKSNAYKEMKKTKLKEMYMVR
                     YADDFRVFCRYKESAEKAKIAITQWIEQRLKLEVSQEKTRIVNVRKRYSDFLGFKIKM
                     IPRRKKLVVKSHISDKQFKNQKDKLVSQAKKIASPSKGKTELEETRLYNSMVLGMQNY
                     YQIATNVNLDCMKLNRAVMAIFTNRLGTNKRGRLRKTGRQLTTKEATRYGKSKMLRYV
                     AGEGEPIFPVGYIQHRKPMAKVYLANCYTLEGRKFIHTNLSVDKLLMYQLMKLSLENR
                     TIEYADNRISLFSAQHGKCAITLEEFQQANEIHCHHIVPTGAGGNDDYKNLILVKEAV
                     HRLIHAKTEETIQKYIALLKLNKVQLIRLNKYRVLAGNQELNLI"
BASE COUNT          632 a          251 c          307 g          404 t
ORIGIN      
        1 tgcgaagcta aatttagtga aaacagctat ggttttagac ctaataggtc agttgaaaat
       61 gcaataaaag caacctataa ccgcctacaa atcagccagc tacattatgt gattgaattt
      121 gatattaagg gtttctttga caacgtgaac cattccaagc tgataaaaca gatatgggct
      181 atgggaatac gggacaagca cttgatattc attttaaaaa ggatattaaa agctccaatc
      241 aaaatgacta acggtacaat aacatatcca gagaaaggaa ctccgcaagg gggaataata
      301 tccccactac tagcaaacat tgttcttaat gagttagacc attgggtaga aagccaatgg
      361 caggaaaatc ctgttacaaa aaactatgtg gttcatatca ataaaagcgg tagcccatgt
      421 aaaagcaacg cttacaaaga aatgaagaaa acaaagttaa aggaaatgta tatggttcgt
      481 tatgcagatg attttagagt tttctgccgc tacaaggaaa gtgctgaaaa agccaaaata
      541 gcaatcactc aatggattga acaacgatta aaattggaag tatcacaaga aaaaacaaga
      601 attgtaaatg ttagaaaaag atactccgac tttcttggtt tcaagattaa aatgataccg
      661 agaagaaaga agttagttgt taaatcacat ataagtgata aacaattcaa aaaccaaaaa
      721 gacaagctgg tatcacaggc aaagaaaatt gcttctccct caaaagggaa aacggaatta
      781 gaagaaacaa gactttataa ttcaatggta ttaggtatgc aaaattatta tcagattgca
      841 acgaatgtaa atctggattg tatgaaactg aatagagctg taatggctat cttcactaac
      901 agattaggta caaacaaaag gggcagattg agaaaaacag gcagacaact tacgacaaag
      961 gaggcaacaa gatatggtaa atctaaaatg cttcggtatg ttgctggcga gggcgaacct
     1021 attttccctg ttggatatat ccaacacaga aaaccaatgg caaaggtgta tttagcgaat
     1081 tgctacactc tagagggaag aaaatttatc cacaccaatc taagtgttga taaattactg
     1141 atgtaccagt taatgaaatt atctttggaa aatcgcacaa tagaatacgc agacaatcga
     1201 atttcgctat tttctgctca acatggaaaa tgtgctataa ctctggaaga atttcaacaa
     1261 gctaatgaaa tacattgtca tcatattgta ccaactggag ctggtggaaa tgacgattat
     1321 aaaaatctta ttttggtaaa agaggcggta caccgtctaa tacacgctaa aacagaagaa
     1381 acaatacaaa aatatatagc tctattgaag ctaaataaag tccagctcat aagactaaat
     1441 aaatatagag tattagctgg caatcaagaa ttaaacttga tataggtcta ctacataaat
     1501 acttttcaat ataaacacga tggaacgcgg agtgcggtga aagtcgcacg ctccgtgtga
     1561 atggggggaa aagtttgaga tatttataat atca
//
LOCUS       DACKAC010000158         1581 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.163, whole genome shotgun sequence.
ACCESSION   DACKAC010000158 DACKAC010000000
VERSION     DACKAC010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.163"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            128..1297
                     /locus_tag="I0B46_002793"
     CDS             128..1297
                     /locus_tag="I0B46_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAR2085022.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVI
                     FMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRG
                     LEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIAHKVRVRGRKEICEDFKLV
                     YQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIYSTNLIEG
                     FNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRIHKGFKEIQDTLESMI"
BASE COUNT          568 a          254 c          258 g          501 t
ORIGIN      
        1 atatcccata ttttaaaata aaagatagcg taaaatattt tgtgtaaata gaaatttagg
       61 gtagaaaaaa aagtccattc tgtaaaatta aagttaccac acagaaattt tataggagga
      121 ctttctcatg actaaattta ctatagaaat tatgcaaaca ctactcaata aaggggattt
      181 agatgaatta tttcaggatc atttagagcg agcgattaat tcacttctac aagcagagct
      241 aactgctttt ttagattacg aaaaatatga tcgaaatgga ttcaattcag gaaattcccg
      301 taacgggaat tattcacgta catttaaaac agaatatggc gagttgaatt taactattcc
      361 tagagatcgg aatggcgaat tttctcaaca aacactacca gcatataagc gaaccaatga
      421 ttcattagaa acgaccatta tccaattatt tcaaaaaggc attactatgg ctgaaatctc
      481 caaattaatt gaaaaaatgt atggtcacca ctatacgcca caaacaatct ccaacatgag
      541 taaattggtg gctgaagatg ttttagcttt taaagaaaga acgttagaag ccaattattc
      601 cgttatattt atggatgcga cgcatatccc tgtgaagcga cagactgttt cgaaggaagc
      661 agtgtatatt acgattggta ttcgtttgga tggaaccaaa gaagttcttg ggtttactat
      721 tgccccaact gagtctgctt atatttggaa agaagttctt caagatctta gaaaacgtgg
      781 gttagaagaa gttttattag tagtgacaga cggattaagc tgtattgaag aaagtatcca
      841 tagtgtgtat ccgaatgccc aatttcaaca atgttgtgtg catgtatcta gaaatatcgc
      901 tcataaagtt cgtgttcgag gtcgaaaaga aatttgtgag gatttcaaat tggtttacca
      961 agcgaattca aaagaagagg cattggatca catcgacttt atgactagga aatggaaaaa
     1021 gcagtatcca agagtcgtca atttactctt gaatcctgcc ctattaacct tttataattt
     1081 ccctcacgcc atcagacgaa caatttattc gacgaacctg attgaaggct ttaataagca
     1141 gctaaaacga tatactcgaa gaaaagaaca attccctaat gaagaatctc tagagagatt
     1201 cttcatttct caatttaatc aatataacca aaaattttta ggtagaattc acaagggatt
     1261 taaagaaatt caggatacat tagagtcgat gatttaactg taattaacag aatggatttt
     1321 ccatttacat ataattcttg acgctaccat aatctaaata accatcatat tcatctacta
     1381 cttatataat tgagttttat taaacttttt aagtttctat tctatcaaat atttaaacaa
     1441 tctctgcatc cgaaaagact acctcgtata gttttactaa caatattttt attagcgtgt
     1501 tcaaatcctg atttcttgta tcaattattt tctgttcact ttcaataaga aaaaagtctt
     1561 tacttccatg gataataata a
//
LOCUS       DACKAC010000159         1541 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.164, whole genome shotgun sequence.
ACCESSION   DACKAC010000159 DACKAC010000000
VERSION     DACKAC010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.164"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(25..762)
                     /locus_tag="I0B46_002794"
     CDS             complement(25..762)
                     /locus_tag="I0B46_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAR2085023.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            complement(813..>1541)
                     /locus_tag="I0B46_002795"
     CDS             complement(813..>1541)
                     /locus_tag="I0B46_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAR2085024.1"
                     /translation="VTSSISNAINAAKSAIQSVWNSISSWISGIWNGIKNTALNLWNG
                     ITSTISSKVNDGKNAISSGWSNLTGIVSDIFNNVKSTIANIWEGIKKTVSAPIDWIRD
                     KISGIFDNLNISIPHIPLPEFIMEGSFNPLKGQIPHLRVKWHAKGGIFTKPTLLGGMN
                     GVGEAGPEAVLPLKRSVLQEIGDRILSSTSVSSRAQTIQPVNNYEFNFTIDGNADEVT
                     MKQTTQQIIDSITKIQNDNASAWR"
BASE COUNT          467 a          309 c          246 g          519 t
ORIGIN      
        1 taaattatcg atcatctatt tcacctacct taacccgtta cgataaattt gtattttgct
       61 caaaccagtg caattaaaat gattgatatc cacttgcaat gtcggatatt gcatggtctt
      121 cattttgttg gaccgatcta aaatatctcc gtccgattgc tcttcgtagc aaagcatcaa
      181 atcactatca atgactacat cagttcctgc tactaagcct tcgaaactaa acacataatc
      241 atttaagatg aactggcatg aagtagctga aggagtgatg ataatctttg gaaaactttc
      301 ttctaaacta ttattcagca agttaaatga ctgtggttta tctacggtta taggtacatc
      361 ttcttgaact cttgcgaatg gtttcgcagt aatatttaca tcgaactctc cccattcaac
      421 aatatcgttt tctgcatccc caatatcgat agtctggata acgtaataga cgttgggatc
      481 gtcagagaat tctaatttct ttgcatagtt taaccaatga cgcatgatat aaaacgattg
      541 cttgaacgct tgatggtctt ccacatcctc taaatagtta tagtgcaatg taaacgacat
      601 atcttcaaac gagtaatctt gtactaagcc acctaaccta cctaaaacag aagtttcaac
      661 tctctgtctt tttggagaag gtatggttgg tctttcagct aaagccaatt tatgcaaata
      721 atcaggaaat ccatcgatta tagaatgtat acaatcagtc attttttcac atccttttta
      781 atactaaaaa aacaggagaa atactctcct gtttaacgcc atgccgaagc attatcattt
      841 tgaatttttg taatgctatc aatgatttgt tgagttgttt gcttcatagt aacctcatct
      901 gcgttaccat caattgtgaa attgaattcg tagttattca caggttgaat cgtttgtgcc
      961 ctagatgaaa ctgatgtgct actcaagata cgatcaccaa tttcttgcaa aacagatctt
     1021 ttcaaaggta aaactgcttc aggtcctgct tcaccgacac cgttcattcc acctagtaaa
     1081 gttggtttag taaagatacc tcctttagca tgccatttta cacgcaaatg ggggatttga
     1141 ccttttagcg ggttaaagct gccttccatg ataaattccg gtaacggaat atgtggtata
     1201 gaaatattca aattatcaaa aatgccactg attttgtctc taatccaatc aattggagcg
     1261 ctaacagtct ttttgatgcc ttcccatatg ttagcaattg tacttttaac attattgaat
     1321 atgtcggaaa caatacctgt tagattggac caaccgcttg aaattgcatt ttttccatcg
     1381 tttactttag agctaatagt gcttgtaatt ccattccaaa gattcaaagc agtatttttg
     1441 ataccgttcc aaattccgct aatccacgaa gatatactat tccaaacact ttgaatggca
     1501 cttttagctg cgtttatagc attgcttata ctactagtca c
//
LOCUS       DACKAC010000160         1529 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.165, whole genome shotgun sequence.
ACCESSION   DACKAC010000160 DACKAC010000000
VERSION     DACKAC010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.165"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(25..762)
                     /locus_tag="I0B46_002796"
     CDS             complement(25..762)
                     /locus_tag="I0B46_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAR2085025.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFTLNDYVFSFEGLVVGTDIVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            complement(813..>1529)
                     /locus_tag="I0B46_002797"
     CDS             complement(813..>1529)
                     /locus_tag="I0B46_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAR2085026.1"
                     /translation="VTSSISNAINAAKSAIQSVWNSISSWISGIWNGIKNTALNLWNG
                     ITSTISSKVNDGKNAISSGWSNLTGIVSDIFNNVKSTIANIWEGIKKTVSAPIDWIRD
                     KISSIFDNLNISIPHIPLPHFKLSGEFNPLKGKIPTLGVDWYAKGSVFNSPNIIGVGE
                     AGPEAVLPLKRSVLQEIGDRILSSTSVSSRAQTIQPVNNYEFNFTIDGNADEVTMKQT
                     TQQIIDSITKVQNDNASAWR"
BASE COUNT          467 a          309 c          239 g          514 t
ORIGIN      
        1 taaattatcg atcatctatt tcacctacct taacccatta cgataaattt gtattttgct
       61 caaaccagta caattaaaat gattaatatc cacttgcaac gttggatatt gcatggtctt
      121 cattttgttg gaccgatcta aaatatctcc gtccgattgc tcttcgtagc aaagcatcaa
      181 atcactatca atgactatgt cagttcccac tactaagcct tcaaaactaa acacataatc
      241 atttaaggtg aattggcatg aagtagctga aggagtgatg ataatctttg gaaaactttc
      301 ttctaaacta ttattcagca agttaaatga ctgtggttta tctacggtta taggtacatc
      361 ttcttgaact cttgcgaatg gtttcgcagt gatatttaca tcgaactctc cccattcaac
      421 aatatcgttt tctgcatccc caatatcgat agtctggata acataataga cgttgggatc
      481 atcagagaat tctaatttct ttgcatagtt taaccaatga cgcatgatat aaaacgattg
      541 cttgaatgct tgatggtctt ccacatcttc taaatagtta tagtgcaatg taaacgacat
      601 gtcttcaaac gagtaatctt gtactaagcc acctagccgt cctaaaacag aagtttcaac
      661 tctctgtctt tttggagaag gtatggttgg tctttccgct aaagccaatt tatgcaaata
      721 atcaggaaat ccatcgatta tagaatgtat acaatcagtc attttttcac atccttttta
      781 atactaaaaa aacaggagaa atactctcct gtttaacgcc atgccgaagc attatcattt
      841 tgaacttttg taatgctatc aatgatttgt tgagttgttt gcttcatagt aacctcatct
      901 gcgttaccat caattgtgaa attgaattcg tagttattca caggttgaat cgtttgtgcc
      961 ctagatgaaa ctgatgtgct actcaagata cgatcaccaa tttcttgcag cacagatctt
     1021 ttcaaaggta aaactgcttc aggtcctgct tcgccgacac cgataatatt cggagaatta
     1081 aacacactac ctttcgcata ccaatcaaca cccaacgttg ggattttccc cttcaatgga
     1141 ttgaattctc cgctcaattt aaaatgtggt aacggaatat gtggtataga aatattcaaa
     1201 ttatcaaaga tactactgat tttatctctg atccaatcaa ttggagcgct aacagtcttt
     1261 ttgatacctt cccaaatgtt agcaattgta cttttaacat tattgaatat gtcggaaaca
     1321 atacctgtta gattggacca accgcttgaa attgcatttt ttccatcgtt tactttagag
     1381 ctaatagtgc ttgtaattcc attccaaaga ttcaaagcag tgtttttgat accgttccaa
     1441 attccgctga tccacgaaga tatactattc caaacacttt gaatggcact tttagctgcg
     1501 tttatagcat tgcttatact actagtcac
//
LOCUS       DACKAC010000161         1456 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.166, whole genome shotgun sequence.
ACCESSION   DACKAC010000161 DACKAC010000000
VERSION     DACKAC010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.166"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            108..1040
                     /locus_tag="I0B46_002798"
     CDS             108..1040
                     /locus_tag="I0B46_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAR2085027.1"
                     /translation="MLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            1112..>1456
                     /locus_tag="I0B46_002799"
     CDS             1112..>1456
                     /locus_tag="I0B46_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2085028.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQD"
BASE COUNT          640 a          169 c          227 g          420 t
ORIGIN      
        1 atgaaataaa tcaaaagtat gatgaaacat taaaaaatgt ctattcttca cttatcgttt
       61 caaatataga agaaacttca tcttctgaaa taaaaaataa gctttctttg ttaaaattaa
      121 aagaaaagga atatattatt gacgatgctg tttctgtccc tcttatttta aataaacgag
      181 agaaaaataa tttaaagaaa aaaatgctca ttccatttga ttcagaagtg aaaggattac
      241 tcaataaact aactatttct aatatagact cgactaggga aaaaatcata aaaatatttg
      301 ataaaattaa taatttattc aagaatgaca atgctgaatt aaggaagggt cttttagagt
      361 ttaaacttca agaactagaa cttcattact cttatttagt gaaaattacc gaagaaaaag
      421 aacaacaaaa agcaataaaa gaacagatga tagaagagga aaaagttcgt agagaaattg
      481 atcgtgaaaa gaaacgtatt gataaagaac aacgtcaatt caattctgaa atttccaaat
      541 taatcgcata tatgcaaaaa gctaatgcag atgtggaaaa agaattatat gcaaataaaa
      601 ttcaagaact ggaagaaaaa ttaaaagaat tagaagttat acgtgaaaat gtattacaaa
      661 gagaattaaa tactagagcc ggttatgtgt atgtaattag taatattggg tcttttggtg
      721 aagacatata taaaattggt atgactagaa gattagaacc tatggataga ataaaagaat
      781 tgagtagtgc atcggttcca tttgaatttg acgttcatgc gatgattttt tcagaggacg
      841 ctccgtcttt ggaaactaaa ttgcataatc attttagaaa acaagaagtt aataaaatta
      901 atcaaagaaa agaatttttc aaagtttcac tagacgaaat agagaaagtt gttttagaaa
      961 attataatgg aacagtaaca tttacaaaat tagctaaagc tgaacaatat agacgatctt
     1021 tagaattgtc aaaagattaa atatataaaa agccttcggg ctttttcttt taacaattaa
     1081 taggaacgta tattcgaaag gaatagaaat aatgtggtta gaaaaaataa atgacaaaaa
     1141 atttaagtac accgaacgtt atacggatcc cttaactgaa aaaaagagaa aagtgtccgt
     1201 tactttatct agcaattcac gccaagcttg gaatcaagct aacttacttt tgaatgaaaa
     1261 aatcgcagaa aaaataaaac gaaatgaaga attaccacta acttttggag aattaaaaac
     1321 taaatgggat gaaaaataca agccaactgt aaaagaaagt tcctatcgaa caactcaggt
     1381 ttatctatca ttaatttcaa aatatataaa agatgatgta cttgtaaaaa acgttaatag
     1441 taatcttata caagat
//
LOCUS       DACKAC010000162         1422 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.167, whole genome shotgun sequence.
ACCESSION   DACKAC010000162 DACKAC010000000
VERSION     DACKAC010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.167"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            6..980
                     /locus_tag="I0B46_002800"
     CDS             6..980
                     /locus_tag="I0B46_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAR2085029.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTITPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          497 a          184 c          236 g          505 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtca ctattactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctggactaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgttggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt ttcttgatgt gatatccttg tcttttttgt
     1081 aatgttttta acttattgct tttgcaaaat ttatttacct gttttctgga tcaacgatag
     1141 tgtatattat gctatccttg tttggatttt aggacgttta aagaggcact tttttacata
     1201 acttaatcca tagacctttt aagcatgctg tgagataata ataaagaaga gtttaaagcg
     1261 caccccaaac cacttcccca taagtgtatt acgctttaaa ctcttttata tttgaattca
     1321 ctaagaagca taccatattt ttgaaaaaaa gtgagaaaaa aggcttataa ttaggattta
     1381 gagtaattaa ttagatgttt attttatagg actgatacta ta
//
LOCUS       DACKAC010000163         1409 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.168, whole genome shotgun sequence.
ACCESSION   DACKAC010000163 DACKAC010000000
VERSION     DACKAC010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.168"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..1359
                     /locus_tag="I0B46_002801"
     CDS             <1..1359
                     /locus_tag="I0B46_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAR2085030.1"
                     /translation="LFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLPITEKE
                     HLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEKEGLSS
                     FKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRDNITNW
                     HIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKDSQHPI
                     VYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFFEAIPK
                     GKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWMATIDP
                     VAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHSTSLEG
                     ARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDVQQHFS
                     RN"
BASE COUNT          466 a          255 c          299 g          389 t
ORIGIN      
        1 ctttttagtg atgaagaaat ttctgtaagt gttccagaag cagctgccac cattattcca
       61 gtaaaaggac atcaacgagt tgtaggaacg aaagcagaca aaatcaaaca cttacctata
      121 acagagaaag aacatctcct tcctttggaa gaacaatttt gtgaacattg cggttcacag
      181 atgaaagata tcggacggac aaaagtacga gaagaaattc gtttccatca ggctatgttg
      241 gattgtctca cccattatca acatacctat tgttgtaaaa gctgcgaaaa agaaggactt
      301 tcttctttta aaaaagcgat cgttcctaaa cctttgattt ctaatagctt aggttccaat
      361 agtttagtgg cggaaacgat ccggatgaaa tttggtcaga aagttcctgc ctatcggcaa
      421 gagaactatt ggaaacaaac acatgggttg gatatttcga gagataatat caccaattgg
      481 catattaaag cagtgcaaaa cgcgttagac cctttaggag aacgattaag agtctatctt
      541 aatcaagaag agattctaca cggggatgaa acaagttatc gtgtgattga aagtgccaaa
      601 acagatacct attactggca gttttgtaca ggaaaagaca gtcagcatcc catcgtttat
      661 tatcaccacg acgaaagtcg tgctggagat gttccgaaaa cttttctcaa ggagtttaca
      721 gggtatttac actgtgacgg ctatagcggg tataatgccg tggaaagtgt acgacttgtc
      781 tattgtttcg cgcatgttcg gcggaaattt ttcgaagcaa ttccaaaagg aaagaaaaac
      841 acggatattc ccgcagctca agcagtcaaa caattggata aatggtttgt cctagaaaag
      901 aaatggaaag acttttctcc tgagaagcga ttaagctgtc ggcaacaaga actgcgtcca
      961 ttatttattg ctttttatga gtggatggca acgatagatc ctgttgcaaa atccaagttg
     1021 gatgcagccg ttcaatatgc gtgtaaacta cgaagtggtt ttgaacctat ttttgaagat
     1081 ggtcgtttag aactcacaaa taatcgtgcg gagcgaaata tcaaagagct ggtgatagga
     1141 cggaaaaatt ggttgcattc cacaagtctt gaaggagcac gaacttcagg aatcattctg
     1201 agtgtatata aaacagcaga acttaacgga ttgaatcctg taaagtatct agagtttcta
     1261 tttgataaga taccaaatct tcccgtactt tcagcagaaa ctttagatca actgttgcct
     1321 tggaacaagg atgtacaaca acatttttct cgtaactaaa aacgccaaac attcccgatt
     1381 ctattctata ggtcgggtta tttgacgta
//
LOCUS       DACKAC010000164         1393 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.169, whole genome shotgun sequence.
ACCESSION   DACKAC010000164 DACKAC010000000
VERSION     DACKAC010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.169"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..>1393
                     /locus_tag="I0B46_002802"
     rRNA            <1..>1393
                     /locus_tag="I0B46_002802"
                     /product="23S ribosomal RNA"
BASE COUNT          374 a          300 c          424 g          295 t
ORIGIN      
        1 aagtctgaag aggagtcaaa tgcttctttt cttaaggaca agctgtgatg gggagggaaa
       61 taatagtacc gaagttcctg atgtcacact gccgagaaaa gcttctagtg agaaaacagc
      121 ggcccgtacc gcaaaccgac acaggtagtc gaggagagaa tcctaaggtg agcgagagaa
      181 ctctcgttaa ggaactcggc aaaatgaccc cgtaacttcg ggagaagggg tgctgatcat
      241 acgatcagcc gcagtgaata ggcccaagcg actgtttatc aaaaacacag gtctctgcaa
      301 aatcgtaaga tgaagtatag gggctgacgc ctgcccggtg ctggaaggtt aagaggagtg
      361 cttagcgcaa gcgaaggtac gaattgaagc cccagtaaac ggcggccgta actataacgg
      421 tcctaaggta gcgaaattcc ttgtcgggta agttccgacc cgcacgaaag gcgtaacgat
      481 ttgggcactg tctcaacgag agactcggtg aaattttagt acctgtgaag atgcaggtta
      541 cccgcgacag gacggaaaga ccccatggag ctttactgta gtttgatatt gagtgtctgt
      601 accgcatgta caggataggt aggagccgta gaaatcggaa cgctagtttc gatggaggcg
      661 ctggtgggat actacccctg cgttatggcc actctaaccc gcaccactaa tcgtggtggg
      721 agacagtgtc agatgggcag tttgactggg gcggtcgcct cctaaaaggt aacggaggcg
      781 cccaaaggtt ccctcagaat ggttggaaat cattcgaaga gtgtaaaggc agaagggagc
      841 ttgactgcga gaccaacaag tcgagcaggg acgaaagtcg ggcttagtga tccggtggtt
      901 ccgcatggaa gggccatcgc tcaacggata aaagctaccc tggggataac aggcttatct
      961 cccccaagag tccacatcga cggggaggtt tggcacctcg atgtcggctc gtcgcatcct
     1021 ggggctgtag tcggtcccaa gggttgggct gttcgcccat taaagcggca cgcgagctgg
     1081 gttcagaacg tcgtgagaca gttcggtccc tatccgtcgc gggcgttgga aatttgagag
     1141 gagctgtcct tagtacgaga ggaccgggat ggacttaccg ctggtgtacc agttgttctg
     1201 ccaagggcat tgctgggtag ctatgtaggg aagggataaa cgctgaaagc atctaagtgt
     1261 gaagcccacc tcaagatgag atttcccatt tctttaagaa agtaagatcc ctgagagatg
     1321 atcaggtaga taggtcagga gtggaagtac agtgatgtat ggagcggact gatactaatc
     1381 gatcgaggac tta
//
LOCUS       DACKAC010000165         1388 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.171, whole genome shotgun sequence.
ACCESSION   DACKAC010000165 DACKAC010000000
VERSION     DACKAC010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.171"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..1310
                     /locus_tag="I0B46_002803"
     CDS             <1..1310
                     /locus_tag="I0B46_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HAR2085031.1"
                     /translation="SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHY
                     PKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIR
                     AYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDS
                     IEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEY
                     SYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISEC
                     TIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCN
                     HLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEK
                     AKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          597 a          103 c          233 g          455 t
ORIGIN      
        1 aatcattaaa aaaacattat acagagcctt gggaagatga agtttttaga gtaattattg
       61 aatataacaa tgttcctatt ggatatggac aaatatataa aatgtatgat gagttatata
      121 ctgattatca ttatccaaaa actgatgaga tagtctatgg tatggatcaa tttataggag
      181 agccaaatta ttggagtaaa ggaattggta caagatatat taaattgatt tttgaatttt
      241 tgaaaaaaga aagaaatgct aatgcagtta ttttagaccc tcataaaaat aatccaagag
      301 caataagggc ataccaaaaa tctggtttta gaattattga agatttgcca gaacatgaat
      361 tacacgaggg caaaaaagaa gattgttatt taatggaata tagatatgat gataatgcca
      421 caaatgttaa ggcaatgaaa tatttaattg agcattactt tgataatttc aaagtagata
      481 gtattgaaat aatcggtagt ggttatgata gtgtggcata tttagttaat aatgaataca
      541 tttttaaaac aaaatttagt actaataaga aaaaaggtta tgcaaaagaa aaagcaatat
      601 ataatttttt aaatacaaat ttagaaacta atgtaaaaat tcctaatatt gaatattcgt
      661 atattagtga tgaattatct atactaggtt ataaagaaat taaaggaact tttttaacac
      721 cagaaattta ttctactatg tcagaagaag aacaaaattt gttaaaacga gatattgcca
      781 gttttttaag acaaatgcac ggtttagatt atacagatat tagtgaatgt actattgata
      841 ataaacaaaa tgtattagaa gagtatatat tgttgcgtga aactatttat aatgatttaa
      901 ctgatataga aaaagattat atagaaagtt ttatggaaag actaaatgca acaacagttt
      961 ttgagggtaa aaagtgttta tgccataatg attttagttg taatcatcta ttgttagatg
     1021 gcaataatag attaactgga ataattgatt ttggagattc tggaattata gatgaatatt
     1081 gtgattttat atacttactt gaagatagtg aagaagaaat aggaacaaat tttggagaag
     1141 atatattaag aatgtatgga aatatagata ttgagaaagc aaaagaatat caagatatag
     1201 ttgaagaata ttatcctatt gaaactattg tttatggaat taaaaatatt aaacaggaat
     1261 ttatcgaaaa tggtagaaaa gaaatttata aaaggactta taaagattga ttatataata
     1321 tatgaaaagc tattataaaa gacattagta ttaaatagtt taaaaaaatg aaaaataata
     1381 aaggaagt
//
LOCUS       DACKAC010000166         1362 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.172, whole genome shotgun sequence.
ACCESSION   DACKAC010000166 DACKAC010000000
VERSION     DACKAC010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.172"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            82..>1362
                     /locus_tag="I0B46_002804"
     CDS             82..>1362
                     /locus_tag="I0B46_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAR2085032.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          464 a          262 c          257 g          379 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat ta
//
LOCUS       DACKAC010000167         1353 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.173, whole genome shotgun sequence.
ACCESSION   DACKAC010000167 DACKAC010000000
VERSION     DACKAC010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.173"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            153..854
                     /locus_tag="I0B46_002805"
     CDS             153..854
                     /locus_tag="I0B46_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAR2085033.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT          465 a          241 c          240 g          407 t
ORIGIN      
        1 attattcaat accattgtat attttttaca taataacaaa ttatatttgt aaagtattaa
       61 aacttgtttc taatgttggt atgttaaaac tttacaaatt ctttaaaaaa agtgacaata
      121 aatatagtga acaataagga gaggactttt taatgcgtgt taataaaatc gattttcact
      181 tttcaaaact cagatatcaa cctgattttg aacatttctc ggatgaagag ttccaacaga
      241 tcaaggaaca tactgttctt cgttcttata aaaaaggaca gatccttttc gacgatggtg
      301 atcccagaca acggttgtat atcttgacca aaggactcgt tcgtttagaa cgttatgatg
      361 aaagtgcaac ttattactac tatgactatg tgaatacaaa taaccttttt cctatgtcag
      421 gaatgtttgg agaagagacc tacacttata cagctcaagc gatgactgat attgaaacat
      481 tctaccttcc tgtttcctac tatgaaaaat tagcacgaaa aaatcccgaa cagttgatct
      541 atatgataca aaaaatttct aaagtagttg aaatacatga attgcgtatc caaatcggat
      601 tgacctctag cgcctttgac cgggtaaaac aaaatttatt cattttgaaa gaagaattgg
      661 gcgtaccaac aaaagatggt catatcacca ttccttatcc gatcacctta aaagaattag
      721 cagtgaatag cggcacgaca cgtgaaactg ctggacaagt aatcaaacaa ctacgtgatt
      781 ccggactgtt agattatgac aaaaagcgtt ttactttctg taagacagct actactccag
      841 ctatgagaga ataacgcgaa caaaaataag cagtgaccct gatatttatc agggtcactg
      901 cttattttcc tccctaaaac ttctgaaaga tccggcagtt ttagatcact gggcaagact
      961 caaagatttt ctatgtgtgt ttcagttatg taatattcac tgtttgcaaa aaatactgat
     1021 agattaacta gaggaagcgt cttgtcctca tccgacctag attattcgat attcctcgac
     1081 aataaacgat taatcagtga gggcaatcag aggaacggat aatgatgaat aagctgtaaa
     1141 aaatagggct ttgacaaaag cgtgtcacag cccttatttc gattaaacgg tgtgtacctg
     1201 tcaactcttc ggtcacaagt cataatgttc ataaacatga ggagctgttt tttgaaattg
     1261 ttccttgtga atcagagtta aacgacaggc ttacgaactc ttgattaaac ggcgttcaaa
     1321 agcttgcata cgcggtaata agaacagcca ggt
//
LOCUS       DACKAC010000168         1324 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.174, whole genome shotgun sequence.
ACCESSION   DACKAC010000168 DACKAC010000000
VERSION     DACKAC010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.174"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            278..1324
                     /locus_tag="I0B46_002806"
                     /pseudo
     CDS             278..1324
                     /locus_tag="I0B46_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180785.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
BASE COUNT          533 a          157 c          247 g          387 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcaaat tgatgaagca gatcccaaag ggctgcttca tcaatttttt
      121 cgtaagtgtc cgtacactta ctgtgcttgc tcatatatta atcgatcata tactaatcgt
      181 gtaagaacaa ctttaagcat aatacttata ttgttatacc atagtttgga aaaataaaaa
      241 attatatttc atcaacgttt cataaaaggt gtgtagaatg ttattgaaag tagctataaa
      301 atgctattgt gtaaaaaata aaggaggaat aatttatgaa aaaatagtac caagaggagc
      361 atgcctatta cttgcattat gtttgagctt aagcgcagtt gggtgtcaaa aaagtaatga
      421 aaacaataaa ataaaagaag gacaatccat aagttctgaa gaggaaggca tgtcaattaa
      481 tgcaaatggc aaaaatgaaa cttttaagcc atcagattat acattggagg caaagaaaga
      541 atatgtttat gaatatttag ggcttaaatt taaactctca gacaaattta gaaattatat
      601 agctgataaa aagatagcga tgttagatga tcaaagtcct atagataaag aactaaagta
      661 tgctattttg acatttgaga aaatgacaga agaacaaaag aatgcagtta tagaaaagat
      721 gggcgatgga tataaaaatt ggcaaaatga gcttgaaaga attgggacta ttggtatatt
      781 tgaaaaaaat acatcagaag aaaaaaatct aaaattataa aatgtgatac tcatactaaa
      841 ataggagttt caagtgatgg aaaatacgat tgctatttaa gcacaaatag cggagctgaa
      901 agtaatcttc ttgatgaatt aaaaagaact gagattcaaa ttatagataa aaaagaacgt
      961 cctaaaaatg gttttgtgct atcagaaaaa actgatttag aaaatacaga agcatttaac
     1021 aaagaatcgg ttaaagattt acgtaaacta ccaacaaagg acataaatgg caaggatttt
     1081 acaagtaaag attttgagaa gtatgatctt actatggtaa atgtatttgc aacttggtgt
     1141 acagcttgtg taaaggaaat tcctgatttg gttgaggttc agaatgaaat gaaaagcaag
     1201 ggtgtaaata tcgttggagt tgttacagat cctgttgatg ataacggtga aaacaaggaa
     1261 gcaattgaaa agtcaaagtt aatacatgaa aagacaaagg cttcatatcc tttcttaatg
     1321 ccag
//
LOCUS       DACKAC010000169         1302 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.175, whole genome shotgun sequence.
ACCESSION   DACKAC010000169 DACKAC010000000
VERSION     DACKAC010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.175"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(680..753)
                     /locus_tag="I0B46_002807"
     tRNA            complement(680..753)
                     /locus_tag="I0B46_002807"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(717..719),aa:Ile,seq:cat)
     gene            complement(943..>1302)
                     /locus_tag="I0B46_002808"
     CDS             complement(943..>1302)
                     /locus_tag="I0B46_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAR2085034.1"
                     /translation="LTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTG
                     KELARVRADGIYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKG
                     GAQDFHDKRWYLNIPKR"
BASE COUNT          411 a          238 c          220 g          433 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcatga ctgacgtgcc ggacgcacgt agcgatcatc ggcgttatga
      121 ctatataaag tatactttcg ttgatccaga aaggtataaa gattaaagta ggctacctaa
      181 aaaggtagcc cggaacgaat tttatcacca tacttgtgaa aggagatatt ttttaagtta
      241 gtattaagat tgtgtaatat gatgatatct atattttata gtatcagtgc tataaaatca
      301 aaaataagtc actaattaac taccactcca attgtaagcc ttttttctca ctttttttca
      361 aaaatatggt atgcttttta atggcttcaa atataaaaga gtttaaagcg taacacactt
      421 atggggaagt ggtttggggt gcgctttaaa ctcttcttta ttattatctc acaatttaac
      481 ccaaatgtct ttctatttaa aaatcaaagt aaaacttttc aaatatacag aagtataact
      541 atgtgaaaca tcctttcatt aatccataaa aggatacata aaaaagccac tcatttgagt
      601 ggcaatgaag aaaagcttta gcttgtataa tactcttcaa aaaaattcta acacagaacg
      661 attcaaatgg ctacgttaat gtaccctgta ggactcgaac ctacgaccgg acggttatga
      721 gccgtctgct ctgaccaact gagctaaggg tactggttgt tgccacataa agccataaac
      781 aatcaaccag tagaatgtgt ggcaacaaac ctgttatcgc atagcttgga gtgtgactat
      841 ttatgggtga tagtgaagat atgcgataac atcactattt tatcgaataa tttttatagt
      901 tgtcaatata gttatgtact gctcctcaac gaggagctat ttttatcgtt taggaatatt
      961 taaataccaa cgtttgtcat ggaaatcttg cgcaccgcct ttagtgtttc cctctggatc
     1021 attcgttgcc cgcatcatta catagacttt cttactagga aaattacgca tattgaaaga
     1081 tacatgataa ccaacatttc cagaagtatt ataagcttgg tttacatctg gtctataaat
     1141 tccatcagct cttactcgag ctaattcttt cccagtattg tagtccataa tgaaaatata
     1201 ctcgtattta tagttagcaa tgtgccatcc agccacatgc aagtttgcgt tttcgatttc
     1261 cccaaactga tcaatgtggg cgtgatttgt tccatctgtc ag
//
LOCUS       DACKAC010000170         1294 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.176, whole genome shotgun sequence.
ACCESSION   DACKAC010000170 DACKAC010000000
VERSION     DACKAC010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.176"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            141..398
                     /locus_tag="I0B46_002809"
     CDS             141..398
                     /locus_tag="I0B46_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2085035.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEANKYEKYDEKELDKEFKEINDLLL"
     gene            complement(630..1043)
                     /locus_tag="I0B46_002810"
     CDS             complement(630..1043)
                     /locus_tag="I0B46_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HAR2085036.1"
                     /translation="MDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVLSASIPI
                     LIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEKLLYITK
                     TSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
BASE COUNT          489 a          166 c          161 g          478 t
ORIGIN      
        1 ttctgtccac gttcatatta ctccaaagtt ttcctcattc caaaagaaaa gcacagaata
       61 cacaaagaag cgtttttatt tgtataaact atatcaaagt tctatactta aattatcaaa
      121 taaaaaggac gtgaagataa atgtcaaatt tagaaaacaa agaagaaaaa gttgtaaaca
      181 aaattgtttc tgttgtcaat aaactggata aagagttaga cgaacttgat acgttatctg
      241 aaaatccaga aaaaaaacat aacctaaaaa aatggttagt tgaacgaaag gctattcatg
      301 aaattaaaaa agttcttcat gaagctaata aatatgaaaa atatgatgaa aaagaacttg
      361 ataaagaatt taaagaaatc aatgacttgt tactataatt gatttatacg actcattttt
      421 gagtcgtcag actgcaaaca acactctaaa tttaggagtt tgtctacagt ctgaataaat
      481 tttaaattta ttgtccataa tgttattatg aatttatagc attaagaaaa gagataagac
      541 tttacatttt tttaggaatg taaagtctta tctcttttct taattgtaca attagtatta
      601 tctgcactac tttctaatta aataaatttt tagttattaa cttgattaaa atcagagata
      661 atatcattta attcagtttg aagggttgta acgattaaat ggaaattttc ttcatctgtt
      721 ttattatatg gggatgtttt cgttatatat aacaactttt ctttttcaat tttcattaat
      781 aactctgtcg aagtctgtac tttatcttga taattaaatg cagacatccc actttgtata
      841 attgtaatta ttgctgaagc aattgaaaca atcaatagta aagacctgtg ttcggaagcc
      901 tgatcaatta atattgggat actagctgat aagactattt ttataatatt tccgatacca
      961 attactctat tataaaatcg aatttctttt tttaatctat cgattgtaga atcaatagat
     1021 tcgataaacg ctttttgatc catcattttc tctccaatca ttttattaaa atcattaaca
     1081 aaaatattat aataggattt aagtaagttc taactaatca catataaaaa taaaaaaaca
     1141 tttgtatttt atcaaagttt tattatactt gtcaatttgt gtacatccta aaatataagc
     1201 tatttgttta cttctgtaga atatatactt tgttgttaga taagttgcac atgtttatag
     1261 aatctaaata ataaattttt atgtaccgcc cctc
//
LOCUS       DACKAC010000171         1197 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.177, whole genome shotgun sequence.
ACCESSION   DACKAC010000171 DACKAC010000000
VERSION     DACKAC010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.177"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..153
                     /locus_tag="I0B46_002811"
     CDS             <1..153
                     /locus_tag="I0B46_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAR2085037.1"
                     /translation="NKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFSFSVSTEIKV
                     LMGITA"
     gene            159..575
                     /locus_tag="I0B46_002812"
     CDS             159..575
                     /locus_tag="I0B46_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2085038.1"
                     /translation="MEFREGAFDFQTSQQNRKFIPEDFQNLQLENMRLEDLFRLADDD
                     KYRFEVLKIKKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNR
                     NSTDVVMITDIYVEDEVIIKYMKPVIKLVNKNKGNG"
     gene            681..944
                     /locus_tag="I0B46_002813"
     CDS             681..944
                     /locus_tag="I0B46_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAR2085039.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDLKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            944..>1197
                     /locus_tag="I0B46_002814"
     CDS             944..>1197
                     /locus_tag="I0B46_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAR2085040.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTYEGRYSRRLNRQHRVVYKVDEDEHVVEIYSART"
BASE COUNT          455 a          153 c          243 g          346 t
ORIGIN      
        1 aataaatttt atcaaagtct ccgtacagcc tcttccacga ttaagggcat ggagaccatt
       61 cgaggaatat ataaaaagaa ccgaagaaat ggaacgctct tcagcttttc ggtgtctact
      121 gaaatcaagg tattaatggg aataacagcc taagatattt ggagttcaga gagggcgcgt
      181 ttgattttca aacttcgcaa cagaaccgaa aatttatacc ggaagatttt caaaacttac
      241 agttagaaaa catgcgttta gaagatttat ttagattagc agatgatgat aagtatagat
      301 ttgaagtttt gaaaattaaa aagaatgaac tattgaataa gttatatcct gagaatgcga
      361 taaaagcaca ttttggtgat gctaatcata tgtatagttg gttcattcct aaaagattta
      421 gagggaaaat caatgctgga gatcaagtga aagtactcaa tcgaaatagt acagatgtcg
      481 taatgataac agatatctac gtagaagatg aagttataat caaatatatg aagcctgtga
      541 taaaacttgt taataaaaat aaaggaaacg gataacactt tacaatgctg ttttggtact
      601 ccaatttaaa caaaacaatc gaatttacgt acttaattaa gtacgttaga atggagatat
      661 aaattagagg gggagtagat atggcaaaaa cagtcttaaa tcctagctca gcacggaaaa
      721 atttttataa gttgttgaaa gatgtaaatg agaatcatac ggaaattgaa atcattagtg
      781 agcgcagtga aaataatgcg gtgcttattg gtttggatga tttgaaagca atcaaagaaa
      841 cgctattact tgagcaaata ggcactttgg gtgttgttcg taagcgagaa aaagatgata
      901 gtggatttac taatgttgat gaaattgatt gggataactt ataatgaata actattctgt
      961 gatgattaaa aactcggcta aagctgattt aaagaaaatc aaacagtcta atttgaaacc
     1021 tcaatttgag aaggttattc agactttaaa ggaagatccc tatctcccta cccaatcatt
     1081 tgagaagtta aaacctacat atgaaggtag gtattctaga agattgaata gacaacaccg
     1141 ggttgtttat aaggtagatg aagatgaaca cgtggttgaa atttattcag caaggac
//
LOCUS       DACKAC010000172         1194 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.178, whole genome shotgun sequence.
ACCESSION   DACKAC010000172 DACKAC010000000
VERSION     DACKAC010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.178"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            197..>1194
                     /locus_tag="I0B46_002815"
     CDS             197..>1194
                     /locus_tag="I0B46_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAR2085041.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLG"
BASE COUNT          463 a          190 c          251 g          290 t
ORIGIN      
        1 ccaaagctct tagttgaaac gtgggtcgat gtctattcga tgttgaccga cgttttttct
       61 atgccttcag atttggtttt aagcgatatc tgtttagatg acattttgca aatggcttac
      121 aacaagagtg cttatgaagg atggaaaaac tatgcaataa accaatccca gaaaaactaa
      181 agaaaggagg taaaaaatgg ctaaaaagag aacagaagca gaagtaacat tcatagctaa
      241 cgatgacgga ttgaaatcta cgttaaaaga aatcagtgct gaattaacta aaaatagagc
      301 agaattaaaa ctagaacaag ctcaattaca acagactggt tctgaatcag acaagttagg
      361 aagtaaatta tcttctttag aaaagcagta tgaattacaa agtcaaaaag ttgaagtaac
      421 tagccaacgt ttagccaatg ccaaaaaata ttatggagaa aattccaccg aagttcagaa
      481 acttgagaga gaactgatta accaacaaac agcgcaacaa cgtttgtcaa acgaaattga
      541 taaaacgagt aatgcactag ctcaagcaaa aggcgaaata cagacgtacg agtctacaat
      601 gcaacagttg gatagtgaac aaaaaaatgt tcaagctagt gcttctctga ttgaatccga
      661 atacaaaaaa tggcaagcaa ctgctggtca atcagcttct gaatccgaga aattagcgaa
      721 agcccaagaa tatgtttctc aacaatctga aaatgcagag aaaacgatag atatcctgag
      781 acgacagtta gaagctacac agtctgagtt tggcgctaca tccacagaag caatgcagat
      841 ggaggcgaag cttaatgatg ctgaacgtga atttgaagag ttaggacaag ctgctaaaaa
      901 tgtagataca actaacttgg acgatatcgg aagcaaaata gatatgaata atctaatgga
      961 agcttctgac gttttaagcg acattggcga taagcttaca gaattaggga aacaagcagt
     1021 ggactctgct aatagtgtag gtagttccca gagtaaaata caagctaatt ttggtttgac
     1081 taaacaagag gctgaagaat taacgaatgt agccagagac atttattata aaggttttgg
     1141 agaatcgtta gatcagtcca cagatgcatt gattttggta aagcgtaatt tagg
//
LOCUS       DACKAC010000173         1159 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.179, whole genome shotgun sequence.
ACCESSION   DACKAC010000173 DACKAC010000000
VERSION     DACKAC010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.179"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..344)
                     /locus_tag="I0B46_002816"
     CDS             complement(<1..344)
                     /locus_tag="I0B46_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2085042.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLG"
     gene            complement(368..>1159)
                     /locus_tag="I0B46_002817"
     CDS             complement(368..>1159)
                     /locus_tag="I0B46_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2085043.1"
                     /translation="YSKKVNKDIMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNL
                     VFSTWDNGNNRPFLSFGAPNFRYSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKF
                     TSDGMLTLPGCFFGSWEDGKLARFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAY
                     ETPEYYFADYGEAVTGDNGKVRVDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDID
                     HFIIETSKPNVLVRWNLVAHRLGYEDIRLKEDTAYDSTVLDQKRF"
BASE COUNT          347 a          254 c          175 g          383 t
ORIGIN      
        1 cctagtgaga aaaaaatgat atcttgcgtt gttaaaattt gattgtcttt tctatattct
       61 acgtagcagt gtttataata tcctgcttca ctcataaatg tgccatcaag agaaaacgtg
      121 acttcttcac tagttacact agttgcaaca ttatctacgt aacggttaga tggtgttgtt
      181 ccttttaaag taaatgttcc gccactcgta tccatctgca agccatttag aaacggttta
      241 accgtcaccg taatcccttt atcaccttga cgagccataa tagctttggt gtagtttaat
      301 tctttgctga aatctaaagc caaattatat aaactgctag ccatttatat acctccttgt
      361 cttcgtttta aaaacgtttt tggtcaagca ctgtgctatc atatgctgta tcctctttta
      421 atctaatatc ttcataccct agacggtgtg ccactaaatt ccatctaact aatacgtttg
      481 gcttactagt ttcaatgatg aaatggtcaa tatcttcatg agtaacagca cacaaaacta
      541 gttctgtagg tgtcacatgt gtcatatacc gacttagatt tactgtttca gcaaacatgg
      601 ggtcaatatc aacacgaact ttaccattgt cacctgtaac ggcttcccca taatcagcga
      661 aataatattc tggagtttca taagcgttca atagtcgttg tccataatgt tctgttggta
      721 cagttgagtt tttagtacct ctaacagtaa aatctttata tacttgtacc gttgattgtt
      781 caaacctagc aagtttccca tcttcccatg aaccaaaaaa acaacctggt aacgttagca
      841 taccatcact agtaaatttc atagtcctac cagctacctt aaattcccat gagttacccg
      901 cactaccatt aatgcttaaa gaactaccgt cgccagaagt tacataacta gcattgctat
      961 acctgaaatt gggcgcacca aaagatagaa acggtctgtt attaccatta tcccacgtac
     1021 taaaaaccaa gttaccctgt ggatttctaa tcatgaaacc accaccagtt ttcatggtgt
     1081 atgatacaat accggcatca gcacttacat aatcacgtgc ttctagctcc ataatatctt
     1141 tgttaacttt ttttgagta
//
LOCUS       DACKAC010000174         1155 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.180, whole genome shotgun sequence.
ACCESSION   DACKAC010000174 DACKAC010000000
VERSION     DACKAC010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.180"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..143)
                     /locus_tag="I0B46_002818"
     CDS             complement(<1..143)
                     /locus_tag="I0B46_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAR2085044.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQIA
                     NNQ"
     gene            complement(154..351)
                     /locus_tag="I0B46_002819"
     CDS             complement(154..351)
                     /locus_tag="I0B46_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAR2085045.1"
                     /translation="MILPDKYYQVIKWAVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            complement(367..609)
                     /locus_tag="I0B46_002820"
     CDS             complement(367..609)
                     /locus_tag="I0B46_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAR2085046.1"
                     /translation="MKDEAIQDVVERLVRIETKLDNYELLREKAESAKDRADQAYSIA
                     LNNAEDIKEMKANNKWSWGYMIGLGITIIGYFLTKL"
     gene            complement(687..>1155)
                     /locus_tag="I0B46_002821"
     CDS             complement(687..>1155)
                     /locus_tag="I0B46_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2085047.1"
                     /translation="NELDNKIDERINDTATTAFYGAQGEVSNKTKVAEYGVGTGYTTT
                     AVGETHFERQSNGYVKCLKAGKYSISAQIRVQLGGKYASWLYTDLYKDGTSVDNLVSY
                     GVDALQNRFAASGNIVTDLEVNDVLNMRTEIGVAYGILQFTSCRTLVLTRLSD"
BASE COUNT          336 a          255 c          166 g          398 t
ORIGIN      
        1 tactgattat ttgctatttg tgagctacct tcatttgcgc ccaaattaaa ttcattgttg
       61 atagtatagg caaacccatt aattggcaat aaaaaaagag ccgttaatag gctcatcgca
      121 gtaatagtaa ttttcttttt catttgtttc ctcctatttt ttcaaattat aagccgacac
      181 accagtgata acacctaaaa atgttgctac tgcattgata gtgagtactg tcatatctgt
      241 tccattccat ccatacgctt tccctaacgt ggctactaac acagaagcag ctggtaatac
      301 tgttaaaact gcccatttaa tgacttgata atacttatcg ggtaaaatca tttcttctca
      361 cctcctttac aatttagtca agaaatagcc aatgatcgta atgcctaaac cgatcatgta
      421 accccacgac catttattat tggctttcat ttctttgata tcttccgcat tattaagcgc
      481 aatagaatat gcctgatccg ctctatcttt tgcactttcc gctttttcgc gtaataattc
      541 gtaattatcc agtttcgttt caatacgcac taagcgttct accacgtctt gtattgcttc
      601 gtctttcaac caactagcct cctttcattg caaaataaaa aacaccctcc tttgagcgtg
      661 ttgatagcaa aattataaat attgtattaa tcagatagtc tagtcaagac taaagtacgg
      721 caagatgtaa attgtaaaat tccatatgca acgccgattt cagtacgcat atttagtaca
      781 tcgtttactt ctaaatcagt tacaatgttt ccgctagctg caaatctatt ttgcaaggcg
      841 tcgaccccat aagatactaa attatcaaca gaagttccat ctttatataa atcagtatat
      901 aaccatgaag catatttccc gcccaattgc acacgtattt gcgcactaat tgaatactta
      961 ccagctttta agcatttaac gtatccgtta gattgacgtt cgaaatgtgt ttctcctaca
     1021 gctgtagtag tataccctgt tcctactcca tactccgcta ctttcgtttt gttagagact
     1081 tcaccttgtg cgccataaaa tgcggtagta gctgtatcat taatgcgttc atcgatctta
     1141 ttgtctaatt cattc
//
LOCUS       DACKAC010000175         1146 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.181, whole genome shotgun sequence.
ACCESSION   DACKAC010000175 DACKAC010000000
VERSION     DACKAC010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.181"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            191..445
                     /locus_tag="I0B46_002822"
     CDS             191..445
                     /locus_tag="I0B46_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAR2085048.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="I0B46_002823"
     CDS             446..763
                     /locus_tag="I0B46_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAR2085049.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          406 a          173 c          229 g          338 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaagacaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga accgtaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1081 tgacgtaact tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa
     1141 gaggaa
//
LOCUS       DACKAC010000176         1134 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.182, whole genome shotgun sequence.
ACCESSION   DACKAC010000176 DACKAC010000000
VERSION     DACKAC010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.182"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            1..557
                     /locus_tag="I0B46_002824"
                     /pseudo
     CDS             1..557
                     /locus_tag="I0B46_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375951.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            591..>1134
                     /locus_tag="I0B46_002825"
     CDS             591..>1134
                     /locus_tag="I0B46_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2085050.1"
                     /translation="MKEKETDHFYRVEFNENDPAKGLKSYGGEIVTLELIDQLKTLDN
                     QYAAEAGYYKFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELARLCEQSPEKEQEQT
                     VKNEPISKVQNEIKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKE
                     LLNFMSRFPERSFRNQLLIEE"
BASE COUNT          472 a          189 c          240 g          233 t
ORIGIN      
        1 taaacgaaca aatcgaaaag atgtttgaac accaaagaaa gaccaacggt caaccaatga
       61 acgataagag aaatgggcat tcttggttta ggcaacaaga acgtttagaa aataaaatac
      121 aaagtttgag agaagagatc aagcaacaag aaaaacaggt agaaaaactt gaaagacaag
      181 aagagctaaa ggaaatgggt tataacaagt acggtggcct ggacatgacg atcgaaaata
      241 ttccaagaat caaagaagaa atcgaacgct ttgaaaaagg tgaatcgact ttttcagctg
      301 ccacgatccg aaaataccaa cgaaaattgg aaacattaga acagctgaaa gaaaaatcag
      361 agaaaggaaa agaaaatctt cttcctgaag tacaagcgat cattgacagc ggacgagtga
      421 cgcaatggaa aaagaatcca actatctatt ttctgaaagg ctatcgaaaa gtcgctttag
      481 aactcaatga aaacggggga ttcgaggaat ctaaaaaata ccaagccacg tcggaagaag
      541 aaaaggcagc tgttcgtgaa ttacttgaag aagctacacc tactgattct gtgaaggaga
      601 aagaaaccga tcatttctat cgagtagaat tcaacgaaaa tgatccagca aaagggttga
      661 aaagttatgg tggggagatc gtaacactag aattgatcga tcaactcaaa acactggata
      721 accaatacgc cgcagaagca ggctattata aattctattt tgaagaagtg aagaatggag
      781 aagtagtcga tcagatacga atggatctag gggatggcgt caatagtaat caccccatct
      841 atttagagtt agctagattg tgcgaacaat cacctgaaaa ggaacaagaa caaacagtca
      901 aaaatgaacc tatttcaaaa gtacaaaacg agattaaaga gacaaaaaca aaacaaaagg
      961 gttatcaaaa acgaacggca gaagagatta agcaagaagt caaatcactt tcccaacagg
     1021 cgcttgatgc ggtgaaaaag catactcatt cacctgaaga cgtcaaagaa cttttgaatt
     1081 ttatgtctcg ttttccagaa cgatccttta gaaatcaatt attgatcgag gaac
//
LOCUS       DACKAC010000177         1102 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.183, whole genome shotgun sequence.
ACCESSION   DACKAC010000177 DACKAC010000000
VERSION     DACKAC010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.183"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(53..124)
                     /locus_tag="I0B46_002826"
     tRNA            complement(53..124)
                     /locus_tag="I0B46_002826"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(89..91),aa:Glu,seq:ttc)
     gene            complement(136..209)
                     /locus_tag="I0B46_002827"
     tRNA            complement(136..209)
                     /locus_tag="I0B46_002827"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(173..175),aa:Ile,seq:gat)
     gene            complement(222..292)
                     /locus_tag="I0B46_002828"
     tRNA            complement(222..292)
                     /locus_tag="I0B46_002828"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(258..260),aa:Gly,seq:tcc)
     gene            complement(297..369)
                     /locus_tag="I0B46_002829"
     tRNA            complement(297..369)
                     /locus_tag="I0B46_002829"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(334..336),aa:Phe,seq:gaa)
     gene            complement(373..445)
                     /locus_tag="I0B46_002830"
     tRNA            complement(373..445)
                     /locus_tag="I0B46_002830"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(410..412),aa:Asp,seq:gtc)
     gene            complement(464..537)
                     /locus_tag="I0B46_002831"
     tRNA            complement(464..537)
                     /locus_tag="I0B46_002831"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(501..503),aa:Met,seq:cat)
     gene            complement(550..639)
                     /locus_tag="I0B46_002832"
     tRNA            complement(550..639)
                     /locus_tag="I0B46_002832"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(601..603),aa:Ser,seq:tga)
     gene            complement(662..733)
                     /locus_tag="I0B46_002833"
     tRNA            complement(662..733)
                     /locus_tag="I0B46_002833"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(698..700),aa:Glu,seq:ttc)
     gene            complement(755..842)
                     /locus_tag="I0B46_002834"
     tRNA            complement(755..842)
                     /locus_tag="I0B46_002834"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(806..808),aa:Ser,seq:gct)
     gene            complement(867..940)
                     /locus_tag="I0B46_002835"
     tRNA            complement(867..940)
                     /locus_tag="I0B46_002835"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(904..906),aa:Ile,seq:gat)
     gene            complement(954..1024)
                     /locus_tag="I0B46_002836"
     tRNA            complement(954..1024)
                     /locus_tag="I0B46_002836"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(990..992),aa:Gly,seq:tcc)
     gene            complement(1026..1101)
                     /locus_tag="I0B46_002837"
     tRNA            complement(1026..1101)
                     /locus_tag="I0B46_002837"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1066..1068),aa:Asp,seq:gtc)
BASE COUNT          316 a          300 c          256 g          230 t
ORIGIN      
        1 taaaatcatt ctttcagata aaaagaaaaa gatactcaaa aagtatcttc catgacccgt
       61 acgggattcg aacccgtgtt accgccgtga aagggcggtg tcttaaccgc ttgaccaacg
      121 ggccaataaa ataaatgggc ctaaatggac tcgaaccatc gacctcacgc ttatcaggcg
      181 tgcgctctaa ccagctgagc tataggccca taaaaactaa aagcgggtga cgagaatcga
      241 actcgcgaca acagcttgga aggctgtggt tttaccacta aactacaccc gcataatggc
      301 gcgggacaga atcgaactgc cgacacatgg agcttcaatc cattgctcta ccaactgagc
      361 taccgagcca aacggttccg acgggaatcg aacccgcgat ctcctgcgtg acaggcaggc
      421 atgttaaccc ctacaccacg gaaccaatgt ttttagtttt taattgcggg agcaggattt
      481 gaacctacga ccttcgggtt atgagcccga cgagctacca gactgctcca tcccgcgata
      541 atatgaaaaa ggaggataag ggattcgaac ccttgcacgg ttttacccgc ctgacggttt
      601 tcaagaccgt tcccttcagc cggacttggg taatcctcca tgtaaaacta tattaaatta
      661 atgacccgta cgggattcga acccgtgtta ccgccgtgaa agggcggtgt cttaaccgct
      721 tgaccaacgg gccaggtaaa acaaaatatt gaaacggaga aggagggatt tgaaccctcg
      781 cgccggtttc ccgacctaca cccttagcag gggcgcctct tcagccactt gagtacttcc
      841 ccaaaaccaa taagattttg tactaatggg cctaaatgga ctcgaaccat cgacctcacg
      901 cttatcaggc gtgcgctcta accagctgag ctataggccc ataaaaaact aaaagcgggt
      961 gacgagaatc gaactcgcga caacagcttg gaaggctgtg gttttaccac taaactacac
     1021 ccgcatggcg gttccgacgg gaatcgaacc cgcgatctcc tgcgtgacag gcaggcatgt
     1081 taacccctac accacggaac ca
//
LOCUS       DACKAC010000178         1082 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.184, whole genome shotgun sequence.
ACCESSION   DACKAC010000178 DACKAC010000000
VERSION     DACKAC010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.184"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            700..>1082
                     /locus_tag="I0B46_002838"
     CDS             700..>1082
                     /locus_tag="I0B46_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004853597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAR2085051.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMW"
BASE COUNT          413 a          161 c          244 g          264 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 ga
//
LOCUS       DACKAC010000179         1035 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.185, whole genome shotgun sequence.
ACCESSION   DACKAC010000179 DACKAC010000000
VERSION     DACKAC010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.185"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..>1035)
                     /locus_tag="I0B46_002839"
     rRNA            complement(<1..>1035)
                     /locus_tag="I0B46_002839"
                     /product="16S ribosomal RNA"
BASE COUNT          218 a          324 c          234 g          259 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgaca
     1021 cccgaaagcg ccttt
//
LOCUS       DACKAC010000180          997 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.186, whole genome shotgun sequence.
ACCESSION   DACKAC010000180 DACKAC010000000
VERSION     DACKAC010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..997
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.186"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            124..435
                     /locus_tag="I0B46_002840"
     CDS             124..435
                     /locus_tag="I0B46_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2085052.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            425..778
                     /locus_tag="I0B46_002841"
     CDS             425..778
                     /locus_tag="I0B46_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAR2085053.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            768..>997
                     /locus_tag="I0B46_002842"
     CDS             768..>997
                     /locus_tag="I0B46_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2085054.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSE"
BASE COUNT          362 a          142 c          192 g          301 t
ORIGIN      
        1 cgatgaagat ttatttttca aagggagata cgtgacttct acgccttttg atgaagattt
       61 atcatttgta aacaagaaaa aatgctctta cgcatatgtt aaagcaatta aggcggtggt
      121 tagatgacgc ctgaacaagt gactgaagaa ttgctaatag ctgtgaagga taatatttac
      181 gttacctgga acgaagaaga tgagtcaatt aaaaagatga tagctaaaaa tgctgtttat
      241 cttcaaagta aagtgagtac aacactttct ttttctcctg aaagcttaga atacggattg
      301 ctaatcgaaa gatgtagata cgactggaat cgtgctttag atgagtttga acaaaatttc
      361 gctagtgagt tattaggttt cattcaacat tatgcgctac aagaatatat tgcaggtgat
      421 gtgaatggcg aataatcgta gactcgaaga aacattcaac gatggttggt taaagatttt
      481 gacgcaaact accaaaagaa atgaactagg aaaaaagatt ggtgtagaag atacagaaat
      541 cacttcttta aaatttagaa atctttccat gagagatagt gatataacag ctatggatgc
      601 gatgggatcg aaattaacta agaaagtaaa gactccattt catccaatcg ccaagaaatt
      661 taataaagat caatatttta tcgtaatcga tagtatgcgt tacaacgtta tctatgccga
      721 ttacgataat ttttatatct atttttatct tgaaagtgtg ggtgaatatg gtgattgata
      781 attctaaaga aaaagaacgt ttaaataagc aaatttctgc tatcaaaact tccttagaag
      841 agcattttgg cctcaaactc tttcaagact ccgtaggcga ggatgagcta cctgatgatt
      901 ttaattactt cattctcgaa acaggagaaa tagaaatgat cactgagcca aaatatagcg
      961 tgggtcaaaa tctatatcta actttctatt cagaaaa
//
LOCUS       DACKAC010000181          991 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.187, whole genome shotgun sequence.
ACCESSION   DACKAC010000181 DACKAC010000000
VERSION     DACKAC010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.187"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(21..94)
                     /locus_tag="I0B46_002843"
     tRNA            complement(21..94)
                     /locus_tag="I0B46_002843"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ile,seq:cat)
     gene            complement(108..181)
                     /locus_tag="I0B46_002844"
     tRNA            complement(108..181)
                     /locus_tag="I0B46_002844"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(145..147),aa:Met,seq:cat)
     gene            complement(209..281)
                     /locus_tag="I0B46_002845"
     tRNA            complement(209..281)
                     /locus_tag="I0B46_002845"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(246..248),aa:Ala,seq:tgc)
     gene            complement(301..374)
                     /locus_tag="I0B46_002846"
     tRNA            complement(301..374)
                     /locus_tag="I0B46_002846"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(338..340),aa:Pro,seq:tgg)
     gene            complement(384..457)
                     /locus_tag="I0B46_002847"
     tRNA            complement(384..457)
                     /locus_tag="I0B46_002847"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(421..423),aa:Arg,seq:acg)
     gene            complement(468..553)
                     /locus_tag="I0B46_002848"
     tRNA            complement(468..553)
                     /locus_tag="I0B46_002848"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(517..519),aa:Leu,seq:taa)
     gene            complement(570..641)
                     /locus_tag="I0B46_002849"
     tRNA            complement(570..641)
                     /locus_tag="I0B46_002849"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(607..609),aa:Gly,seq:gcc)
     gene            complement(653..725)
                     /locus_tag="I0B46_002850"
     tRNA            complement(653..725)
                     /locus_tag="I0B46_002850"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(690..692),aa:Thr,seq:tgt)
     gene            complement(743..824)
                     /locus_tag="I0B46_002851"
     tRNA            complement(743..824)
                     /locus_tag="I0B46_002851"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(788..790),aa:Leu,seq:tag)
     gene            complement(832..904)
                     /locus_tag="I0B46_002852"
     tRNA            complement(832..904)
                     /locus_tag="I0B46_002852"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(869..871),aa:Lys,seq:ttt)
     gene            complement(908..980)
                     /locus_tag="I0B46_002853"
     tRNA            complement(908..980)
                     /locus_tag="I0B46_002853"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(945..947),aa:Val,seq:tac)
BASE COUNT          260 a          283 c          235 g          213 t
ORIGIN      
        1 tgaaacttac aacgccacaa tggaccttgt aggactcgaa cctacgaccg gacggttatg
       61 agccgtctgc tctaaccaac tgagctaaag gtccaggctc ttaaaaatcg cggcggaggg
      121 gatcgaaccc ccgacctccc gggtatgaac cggacgctct agccagctga gctacaccgc
      181 gaaaaagata ttcattattt aaatgaaatg gagcctagcg ggatcgaacc gctgacctcc
      241 tgcgtgcaaa gcaggcgctc tcccagctga gctaaggccc cgtgtttaaa tttataatta
      301 tcgggaagac aggattcgaa cctgcgaccc cttggtccca aaccaagtgc tctaccaagc
      361 tgagctactt cccgtttaat taacgcgccc aagaggagtc gaacccctaa ccttttgatc
      421 cgtagtcaaa cactctatcc agttgagcta tgggcgcata tttgaaatgc cgaggaccgg
      481 aatcgaaccg gtacggtgat cactcaccgc aggattttaa gtcctgtgcg tctgccagtt
      541 ccgccacccc ggcgcgattg ctttggcaaa gcggaaaacg gggttcgaac ccgcgacccc
      601 caccttggca aggtggtgct ctaccactga gctatttccg cgtgtttaaa gatgccggct
      661 aaaggacttg aaccctcgac cctctgatta caaatcagat gctctaccaa ctgagctaag
      721 ccggcatgta aaacttaaat tatgcgggtg aagggacttg aacccccacg ccgtaaggcg
      781 ctagatccta aatctagtgc gtctgccaat tccgccacac ccgcaaaaat atgagccgta
      841 cagggctcga acctgtgacc ctctgattaa aagtcagatg ctctaccaac tgagctaacg
      901 gctcatatgg aggttaacgg gatcgaaccg ctgaccctct gcttgtaagg cagatgctct
      961 cccagctgag ctaaacctcc atggataact t
//
LOCUS       DACKAC010000182          941 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.188, whole genome shotgun sequence.
ACCESSION   DACKAC010000182 DACKAC010000000
VERSION     DACKAC010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..941
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.188"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..>941
                     /locus_tag="I0B46_002854"
     CDS             <1..>941
                     /locus_tag="I0B46_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2085055.1"
                     /translation="QADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQITDLTGQ
                     AKTLQDKLDALKEEISKLGNLQVMYSNSIDFGDYDYSENPNLMPYITEPWVGPLLGNG
                     HTVKDSVKRVITHTKTRTANSGDILSLGLGIPCTAEANNRYLITTLRPSTTYTLSVTM
                     SVGSDWTGETNTIGVRLRYLNEQGGIELPINALIPANVERDKMVTHTFTGITKDNVTS
                     ITNCYVEIFSLNSEYKGTVSVSYDVKLKAHYPNLLDGPYWLGKVPLGENIADPTVVFP
                     HKTSEYMVYGRRNTENYIADQTYTISMKATKLTVQSFA"
BASE COUNT          332 a          160 c          183 g          266 t
ORIGIN      
        1 caagccgatg aatatgtttc gcaattagaa gagttgattc gaaagtacaa cgaaactttt
       61 gatgctttta tggctgaaat caaaggtaga gtggatagct taaatcaaca gattactgat
      121 ttaactggtc aagctaaaac gctacaagac aagttagatg ctctgaaaga agaaatttct
      181 aagttaggta acttacaagt gatgtacagt aacagcatcg acttcgggga ctatgattat
      241 tcggagaacc cgaatttaat gccatatatt acagaacctt gggttggacc tttgttaggt
      301 aatggacata cagttaaaga tagtgttaaa agggttataa ctcatactaa aactagaaca
      361 gctaattccg gcgacatatt atcgcttggc ttaggtattc cgtgtacagc ggaagctaat
      421 aataggtatc ttataaccac tttacgacca tctacgacat acacattaag cgttactatg
      481 agtgtcggtt cagattggac tggagagacc aatactatag gggttaggct tagatattta
      541 aatgaacaag gtggtataga actgcccatt aatgcattaa taccagcaaa tgtagaacgc
      601 gataaaatgg tcactcatac atttactggt ataacaaaag ataatgtaac tagcataaca
      661 aactgttatg ttgaaatatt ctcacttaat agtgaatata aaggaacggt tagtgtcagc
      721 tatgacgtta agttaaaagc acactaccct aacttactag atggccctta ctggttaggt
      781 aaagttcctt taggcgagaa tattgctgac cctacagttg tctttccaca caaaactagt
      841 gaatatatgg tttatggtag acgcaataca gaaaactata tcgctgacca aacatatacc
      901 atatcaatga aagcaactaa actaacagtt caatcgtttg c
//
LOCUS       DACKAC010000183          932 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.189, whole genome shotgun sequence.
ACCESSION   DACKAC010000183 DACKAC010000000
VERSION     DACKAC010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.189"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..>932
                     /locus_tag="I0B46_002855"
     CDS             <1..>932
                     /locus_tag="I0B46_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAR2085056.1"
                     /translation="TIWSALGEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQ
                     ELNGKIAELKDSLAPIGNTIIDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAA
                     FTLLMPIIVGFMALGGPTTLIIGAVITVIAGVIAIIKNWGAITDWFKGIWSKFTDWLG
                     GTWESIKEGASSVWDGVKETWSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQ
                     ETWQAFVDWVSNIWNGVKEVWSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAG
                     QLLGAAWQAVWTPISDFLKNTWDTMTQWVSIAWNG"
BASE COUNT          280 a          140 c          227 g          285 t
ORIGIN      
        1 acaatttggt ctgcacttgg cgaagataac gcgatgaaag ttgtccaatc tttgactgat
       61 gttaaccata catttgatga tgttagtgga tctgcacaaa aaatgaatga agattctact
      121 actccgttac aagaattaaa cggaaaaata gctgaattaa aggattcatt agctcctata
      181 ggcaacacaa tcatagatgc actcgaacca gtaattgatt ttctaggaaa gatggctgat
      241 gcgtttaata atcttccaca accagtacag gattatgccg tagcgattgg cggattgact
      301 gctgcattta ctttattaat gccaataata gttggcttca tggctctagg tggtcctact
      361 acattaataa taggagcagt tattactgtt attgctggag ttatagcaat tataaaaaac
      421 tggggcgcaa ttactgactg gtttaaggga atatggagta aattcactga ttggttgggt
      481 ggtacttggg aaagtataaa agaaggtgcc tcatcagttt gggatggagt taaagaaacc
      541 tggtctggat ttgtagattg ggttcaagat atttggcaag gagtttctga ttggtttgga
      601 gagctatgga gcggattagt tgaaggagct tccaacatct ggcaaggagt ccaagagact
      661 tggcaagcat tcgttgattg ggtttcaaat atttggaacg gagtcaaaga agtatggtcg
      721 attatttggg cagacattgt aggaattgtt caaataccat ggaccttaat aacgtcattg
      781 attcaagccg gtattaatat tatcgtgggt atttttgatg tagctggaca gttattaggc
      841 gcagcttggc aagctgtttg gacacctatt tctgatttcc ttaaaaacac ttgggatact
      901 atgacacaat gggtaagcat cgcttggaat gg
//
LOCUS       DACKAC010000184          932 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.190, whole genome shotgun sequence.
ACCESSION   DACKAC010000184 DACKAC010000000
VERSION     DACKAC010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.190"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..>932
                     /locus_tag="I0B46_002856"
     CDS             <1..>932
                     /locus_tag="I0B46_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAR2085057.1"
                     /translation="TIWSALGEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQ
                     ELNGKIAELKDSLAPIGNTIIDALEPVIDFLGKMADAFNNLPQPVQDFIVAIGGLTAA
                     FGILLPAILAVSYLFGPMMLIIGGIIAVIAGVIVAIKNWGAITDWFSGLWKKFTDWLG
                     DTWESIKDGASSVWDGVKETWSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQ
                     ETWQTFVDWVSNIWNGVKEVWSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAG
                     QLLGAAWQAVWTPISDFLKNTWDTMTQWISIAWNG"
BASE COUNT          273 a          134 c          232 g          293 t
ORIGIN      
        1 acaatttggt ctgcgcttgg cgaagataat gcgatgaaag ttgtccaatc tttaactgat
       61 gttaaccata catttgatga tgttagtgga tctgcacaaa aaatgaatga agattctact
      121 actccgttgc aagagttgaa cgggaaaata gctgaattaa aggattcatt agctcctata
      181 ggcaacacaa tcatagatgc actcgaacca gtaattgatt ttctaggaaa gatggctgat
      241 gcgtttaata atcttccaca accagtacag gatttcattg ttgctatagg tggtcttaca
      301 gcagcatttg gcatactgtt accggcaata ctagccgtgt catatttatt tggtccgatg
      361 atgcttataa ttggaggaat tatagctgtt atagcaggcg ttattgtggc aattaagaac
      421 tggggtgcaa ttactgactg gtttagtggt ttatggaaaa aatttactga ttggttgggt
      481 gatacttggg aaagtataaa agacggagcc tcatcagttt gggatggggt taaagaaacc
      541 tggtctggat ttgtagattg ggttcaagat atttggcaag gagtttctga ttggtttgga
      601 gagctatgga gcggattagt tgaaggagct tccaacatct ggcaaggagt ccaagaaact
      661 tggcaaacat tcgttgattg ggtttcaaat atttggaacg gagtcaaaga agtatggtcg
      721 attatttggg cagacattgt aggaattgtt caaataccat ggacattaat aacgtcattg
      781 attcaagctg gtattaatat tatcgtgggt atttttgatg tagctggaca gttattgggc
      841 gcagcttggc aagctgtttg gacacctatt tctgatttcc ttaaaaatac ttgggatact
      901 atgacacaat ggataagtat cgcttggaat gg
//
LOCUS       DACKAC010000185          907 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.191, whole genome shotgun sequence.
ACCESSION   DACKAC010000185 DACKAC010000000
VERSION     DACKAC010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.191"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..790
                     /locus_tag="I0B46_002857"
     CDS             <1..790
                     /locus_tag="I0B46_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAR2085058.1"
                     /translation="IRTKQHFLENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTL
                     DELAELPLEAFCDLLQEKGKGRFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVL
                     VREIRALEKNIKELDKAIEQVIVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQ
                     TKLAKYAGLSWKVNQSGNYQSQNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQS
                     KYKEVPKHQHKRALVLTARKFTRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          325 a          176 c          160 g          246 t
ORIGIN      
        1 gattcgtaca aagcaacatt ttcttgaaaa tctaacgtac aaatgtaata cgcttgctcg
       61 agaaatgcga gacgaatcga cgagtctttt tagtgcaacc ctcatttcac tcatgacgga
      121 agatttcaca ttggatgaac ttgctgagct tcctttagaa gctttctgtg atttacttca
      181 agaaaaagga aaaggccgtt tcaagcaacc agaaaaaatc gctaaagcaa ttcaacgtgc
      241 aattaccatg agttaccgcc taggtgctct tgcacaagaa tcaattaatg ttgttttaag
      301 tgttctcgta cgagaaatac gagcacttga aaaaaatatc aaagaattag ataaagccat
      361 cgaacaagta attgttgtca ttccagaata tcaatgctta accagtattc caggcgttgg
      421 taaagtctat gctgccggtt taatcgctga aattggtcaa atcgaaagat ttgaagatca
      481 gacaaaattg gcaaaatacg ctggattaag ttggaaagtg aaccaatctg gaaactatca
      541 gtcgcaaaat acacctctga caaaacaagg aaatcgatat tttagatact acttagttga
      601 agctgccaac tctgtaaaaa attatcttcc tgaatacaaa gctttttatc aaagtaaata
      661 taaggaggtt ccaaagcatc aacacaaacg tgcactcgtc ctaaccgcaa gaaaatttac
      721 gcgtctggtg gatacgctac tacgtaaaca ccaactctat acgccaccaa ggagtgtgat
      781 agagaaataa caatccgtta ttgacgcaaa tccttgaatc aacccgaaaa aaatttgatt
      841 ttgatcgggt ctagtttcgt gcgcttcaaa acatattact caataaagaa aatcttaaat
      901 ttcatct
//
LOCUS       DACKAC010000186          905 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.192, whole genome shotgun sequence.
ACCESSION   DACKAC010000186 DACKAC010000000
VERSION     DACKAC010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.192"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..>905
                     /locus_tag="I0B46_002858"
     CDS             <1..>905
                     /locus_tag="I0B46_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2085059.1"
                     /translation="QADEYVSQLEELIRKYNETFDAFMAETKGRVDSLNQQITDLTGQ
                     AKTLQDKLDALKEEISKLGNLQVMYSNSIDFGGYDYSGNPNLMANINADSFSQGTGAL
                     SVIDDGDEVVVTLDPNHKLQLYKMKSQPALVVGETYNMSVEIMLEDDFTGDPSKIALR
                     YFKMPNWVPALTTPNALTTAKGVWQKLTVTAKMTTAIDNAESWYVTLYNQDANNSLSG
                     RLRLRHAKLEEGSTATPYQPNLLDAPYYLSKVPLGKNIADPTVGFPHNTSEYMVYGGR
                     NTENYIAGQTYTLSMKATKLASQSFA"
BASE COUNT          312 a          166 c          184 g          243 t
ORIGIN      
        1 caagccgatg aatatgtttc gcaattagaa gagttgattc gaaagtataa tgaaactttt
       61 gatgctttta tggctgaaac taaaggtaga gtggatagct taaatcaaca gattactgat
      121 ttaactggtc aagctaaaac gctacaagac aagttagatg ctctgaaaga agaaatttct
      181 aagttaggta acttacaagt gatgtacagc aacagcatcg acttcggggg ctatgattat
      241 agtgggaatc cgaatttaat ggctaatata aacgctgaca gcttctcgca aggtactggt
      301 gctttatctg ttattgatga tggtgacgag gtagtggtca cgcttgaccc aaaccataaa
      361 ttacagttat acaaaatgaa aagtcaacca gctctggtag taggcgaaac atataacatg
      421 agcgtagaaa ttatgttaga agatgatttc actggagacc ctagtaagat agcactaaga
      481 tattttaaaa tgcctaattg ggtcccagca ctaactacgc ctaatgcatt aactactgct
      541 aagggtgtat ggcaaaaact aactgttacc gctaaaatga ctactgcgat tgataacgct
      601 gaaagttggt atgtaacact atataatcaa gacgctaata acagcctatc tggtagacta
      661 cgtttgagac acgctaaact tgaagaaggc tcaacagcta caccatacca gcctaattta
      721 ctagacgcac cgtactattt gagtaaagtg ccgttaggta aaaatattgc tgaccctaca
      781 gtcggctttc cgcacaacac tagtgaatat atggtttatg gtggacgcaa tacagaaaac
      841 tatatcgctg gccaaactta taccttatca atgaaagcaa ctaaactagc aagtcaatcg
      901 tttgc
//
LOCUS       DACKAC010000187          900 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.193, whole genome shotgun sequence.
ACCESSION   DACKAC010000187 DACKAC010000000
VERSION     DACKAC010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.193"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            122..526
                     /locus_tag="I0B46_002859"
     CDS             122..526
                     /locus_tag="I0B46_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2085060.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            538..>900
                     /locus_tag="I0B46_002860"
     CDS             538..>900
                     /locus_tag="I0B46_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAR2085061.1"
                     /translation="MAKTKTVTTFDNISIKRISFNFKNATNAISTDCNGQLDGETEMQ
                     RIVKKCGSTEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDERIKSGIYSYGPDSVG
                     EDFSLAAEIVDDFEEKNKL"
BASE COUNT          336 a          134 c          181 g          249 t
ORIGIN      
        1 attcaaaatc gttcgattcg ttttcagaga atggatccca atcatttaaa actagagaac
       61 caagatcgct atatcgatca attggtattt acgtttagac gattattgaa gagtgattgt
      121 catggctaaa aatagttggg agctaaaaat aaatggacat gatgaacttc ttgtgcggat
      181 ggaacgctat tcaagcgaga gcgaacgact gattaacgaa gcattgaaat caaaaggttc
      241 ggctattgca gtggatagga ttacagaaaa aattcctgtt tctgaagcag atttaagaag
      301 agggcaccaa cacgcaaaaa atagtcgtcc acttaagact caatacatta atttgggttt
      361 catcattaga cctacaagaa aatttgagta tttaaaatat cctgatttgg ggataggtac
      421 ttctaaaaga aatcagccgg acgaatttat gagaagagga ttaggtcttg cacttgatcc
      481 aattacagaa cttctgattc gtcaattcga taaattaaat aaataggggg aacaacaatg
      541 gctaaaacaa aaactgtaac aacttttgac aatataagta ttaaaagaat ttcttttaat
      601 tttaagaatg caacaaatgc aatttcaact gattgtaatg gacaattaga tggcgaaaca
      661 gaaatgcaga gaattgtaaa aaaatgcggt tcaacagaag taaaatcgaa atctaaacca
      721 atcaatatga cggtaacaat tactgcacat gtaccgatgg aagtttatcg acgcttcaat
      781 gggttgaaac aagatgaacg tattaaatcg ggaatttact cttatggtcc tgattccgta
      841 ggggaagatt tctctcttgc tgcagagatc gtggatgatt tcgaagaaaa aaataagctg
//
LOCUS       DACKAC010000188          890 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.194, whole genome shotgun sequence.
ACCESSION   DACKAC010000188 DACKAC010000000
VERSION     DACKAC010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.194"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..888
                     /locus_tag="I0B46_002861"
     CDS             <1..888
                     /locus_tag="I0B46_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAR2085062.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT          386 a          116 c          144 g          244 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt
//
LOCUS       DACKAC010000189          860 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.195, whole genome shotgun sequence.
ACCESSION   DACKAC010000189 DACKAC010000000
VERSION     DACKAC010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.195"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..856
                     /locus_tag="I0B46_002862"
     CDS             <1..856
                     /locus_tag="I0B46_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAR2085063.1"
                     /translation="KRFYRPLNPFKQTLPKVLCFDEFKSVRSVASAMSFIMMDGQSHA
                     LLDIVENRRLPYLERYFSRFSLATREAVQLIVIDMYAPYVSLVKKLFPNAQLIIDRFH
                     IVQHIGRTFLNHRVKETTVRLKGNSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRA
                     SFKDYLTESEIADRLLAACPNLRQGYQLYQDILYAVKKRDQSLFEDCLTRDISGLPET
                     YTTTLRTFKKFLPQIQNALHYSYSNGPLECLNNHIKVLKRNAYGFRNFYNFKLRIMIR
                     HGKTFLTK"
BASE COUNT          273 a          173 c          165 g          249 t
ORIGIN      
        1 gaagcgattc taccgcccat tgaatccttt caaacagact ttgccaaagg tcttatgctt
       61 tgatgaattc aaatcggttc gaagtgttgc cagtgcaatg agttttatca tgatggacgg
      121 acaaagccat gcattattgg atattgtcga aaatcgacgc ttgccttatt tagaacgcta
      181 tttttctcgg ttttcattag cgactcgtga agccgttcaa ttgattgtta ttgatatgta
      241 tgcgccttat gtttcgttgg tgaaaaagct tttcccgaat gctcagttga tcatcgatcg
      301 tttccacatc gtccaacata ttggcagaac tttcttgaat catcgagtga aagaaaccac
      361 ggtacgactt aaaggaaatt cccactctca acgagggtta gggaaaaaat tgaagcgcta
      421 ttggaaactc ctacaaaaag aagaagcaaa gttaaactat gaaaaacggg tttggcgtgc
      481 cagttttaaa gattatttga ctgaaagtga gattgccgat cgactgttag ctgcttgtcc
      541 caacttacgc caaggctatc agttatacca agacattctt tatgcggtaa agaaaagaga
      601 tcagtcacta tttgaggatt gtttaactag agacattagc ggtctgccag aaacctatac
      661 aaccacttta cgaacgttta aaaagtttct gccacagatc caaaatgcgc ttcactacag
      721 ctattcaaac ggaccattgg aatgcttaaa caaccacatc aaagtattaa aacgcaatgc
      781 gtacggcttt cgcaactttt acaatttcaa actacgaatt atgattcgtc atggcaaaac
      841 atttttaacc aaataaacag
//
LOCUS       DACKAC010000190          837 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.196, whole genome shotgun sequence.
ACCESSION   DACKAC010000190 DACKAC010000000
VERSION     DACKAC010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..837
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.196"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..750)
                     /locus_tag="I0B46_002863"
     CDS             complement(<1..750)
                     /locus_tag="I0B46_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAR2085064.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGA"
BASE COUNT          244 a          183 c          142 g          268 t
ORIGIN      
        1 cgcacctaaa aagttcttgc gaacagcctt aactaaccct ttgtgtgcat cagaaatgac
       61 cagttttaca ccgaacaaac ctcgttgaac gaacgattga aaaacggtat cccaattatc
      121 ttccgattcg ccgtcttgaa catcaaaacc aagaatcgta cgtcgtccat cagaatcaat
      181 gccgatacca atatatacac cttttgagac aatacgatga ttttccctta ctttcatata
      241 cagggcatca cacatgaaaa atggatattc cgagccttct aggctccggt ttctccactc
      301 aaaaacaaat ggatccaact gtttcatcag atttgaaacg aatgatttag aaacagtcgc
      361 accatccgtt agcagttcaa ctgttttagt tactttgcga gtggagacac cagaaatgac
      421 catttcaatc atggcagcga tcaaagcttt ttcgcttcgc tgatatcttt caaagatagt
      481 aggactaaac ttaccgtctc tggttcgtgg aacatgaagt tctaacgtac caattcgagt
      541 tgtaaacgaa cgatcgtagt aaccatttct ttgtgattgc cgttcttctg aacgttcata
      601 ggcatttgcc tgaatatatt gatttcgttg ttcttccatc agctgattaa acatgatagt
      661 cataagtgtt tttgcggtat cattcgcacc gctttcgttt ataattgctt gaatttcttc
      721 gctttcaaaa gtaaaatgta cttgagtcat tgatatcagt cctattcgag tatgttttct
      781 gcaaaaaaca ttgtactcct aactgagatc aatggctttt ttgatgcttc actttta
//
LOCUS       DACKAC010000191          823 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.197, whole genome shotgun sequence.
ACCESSION   DACKAC010000191 DACKAC010000000
VERSION     DACKAC010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.197"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..>823
                     /locus_tag="I0B46_002864"
     CDS             <1..>823
                     /locus_tag="I0B46_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HAR2085065.1"
                     /translation="GTLELKVPRTRDGHFSPTVFERYQRNEKALMASMLEMYVSGVST
                     RKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKNYPYLMTDVLYIKVRE
                     ENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEYLKERGLQGTELVISD
                     AHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFREAVKGIFKFTDINLAR
                     EAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRLKSTNLIERLNQEV"
BASE COUNT          293 a          157 c          152 g          221 t
ORIGIN      
        1 taggcacgct agaattaaaa gtacccagaa cacgtgatgg ccatttttca cccacagtgt
       61 ttgaacgtta tcaacgaaac gaaaaagccc tcatggcttc aatgttggaa atgtatgtat
      121 caggcgtttc aactcgtaaa gtatcaaaaa ttgtggaaga actttgtggt aaatccgtct
      181 ctaagtcctt cgtttctagc ttaacagaac agctagaacc tatggttaac gagtggcaga
      241 atcgtttatt atcagaaaaa aattatcctt acttaatgac cgatgtactc tatataaaag
      301 tacgagaaga aaatcgagta ctctcaaaaa gctgtcatat agcgattgga ataaccaaag
      361 atggcgaccg tgaaattatc ggcttcatga ttcaaagtgg cgaaagcgaa gagacctgga
      421 caacattttt tgaataccta aaagaacgcg gtttacaagg tacggaactc gttatttctg
      481 atgcgcacaa aggattagtc tctgccatta gaaaatcctt caccaacgta agttggcaaa
      541 gatgccaagt tcacttccta agaaatatct ttaccaccat tcctaaaaaa aattcaaaat
      601 ctttcagaga agctgttaaa ggaattttta agttcacaga tattaactta gcgcgtgagg
      661 ctaaaaatcg attgattcat gattatatcg atcaaccaaa atattcaaaa gcttgcgcat
      721 cattggatga tggattcgaa gacgcctttc aatataccgt acaaggaaat tcccacaatc
      781 gactaaagag taccaatcta attgaacgac tgaatcaaga agt
//
LOCUS       DACKAC010000192          793 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.198, whole genome shotgun sequence.
ACCESSION   DACKAC010000192 DACKAC010000000
VERSION     DACKAC010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.198"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..782
                     /locus_tag="I0B46_002865"
     CDS             <1..782
                     /locus_tag="I0B46_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAR2085066.1"
                     /translation="DMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNG
                     VSLARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGE
                     LISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKL
                     IKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNIDKKVSSHMLRHSHISLLTELGI
                     PLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT          340 a          107 c          102 g          244 t
ORIGIN      
        1 aagatatgct agattactac tattttgaaa aaaatttatc atataattat gtaatacatt
       61 taaaaacttt tacaggaatg atttttaaat tcgccaaccg tcgatatgga ttaaaatata
      121 atcccgtaaa tggggtttct ttagcaagaa aacctaagac aaacgaagaa ataaaaaaag
      181 aaaaagatgg ttatttatca aaagaagaag ttcaaatgat tgctagtaaa caaaaaagtt
      241 cacatcaaca gtcacgttat tctttaatta ctaaattttt gtttttaact ggactacgtt
      301 atggtgaatt gatatctttg actgaatctg attatgatgg taataaaatt acggtttctg
      361 gtacttatga ttacgagtta aaaattaaaa caacaactaa aaatacaggt tcttatagaa
      421 ccattgaatt atcaagtaat gctaaagaaa tcatagatca attaattgaa gaaaacaaat
      481 taattaagaa taataaagat aaatacattt tcatctctaa aaatggtaac ccgatatcta
      541 ttcaagcata taatcaatct ttaagagcag tatccaaaga attaaacata gataaaaaag
      601 tttcttctca catgttaagg cattcccaca tttctctact tacagaatta ggaatcccat
      661 taaaagccat tatggatcgt gtaggacatg aagattcaaa aacaacatta aagatttata
      721 ctcatacaac taaaaatatg caaaatcaac tagtagaaaa actaggaaaa atagaaattt
      781 aaattgcccc ttt
//
LOCUS       DACKAC010000193          785 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.199, whole genome shotgun sequence.
ACCESSION   DACKAC010000193 DACKAC010000000
VERSION     DACKAC010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.199"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            22..714
                     /locus_tag="I0B46_002866"
     CDS             22..714
                     /locus_tag="I0B46_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAR2085067.1"
                     /translation="MGLAFLLAGCTNGNESKKESTKETAKATVATTTTTSTESKSTAA
                     STETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNADKSAQLEAF
                     MTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEWSESGTGQA
                     DYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNETANNELK
                     NGFNQIVYGQAN"
BASE COUNT          282 a          151 c          158 g          194 t
ORIGIN      
        1 aaaaaaagga ttttagtaac aattggtcta gcttttttgt tagctggttg taccaacggg
       61 aatgaatcga aaaaagaatc aacaaaagaa acagcgaaag caacagttgc aactactaca
      121 acaactagca cagagagcaa gtcaactgca gcaagtacag aaacagcaga tacctctact
      181 tcgtcagagt cttccaaaac gactgaaaca actcaaacat ctacttcaag ttcttctgta
      241 gaggcttcat cttcctcttc agaaagcagc gaacaagttg aacaaacact gtggaatgca
      301 gacaaatccg cacaattaga ggcatttatg accaaatggg gccaaacaat gggacaacaa
      361 tacaaaagtt atacgaatca aatgagtgtg gacttatatg gtcttaaagt ccctcaagct
      421 attttgaacg gggagtggaa gatggctatt ggtggagtgc ctgtttctgc agaatggtca
      481 gaaagtggga caggtcaagc agactatcaa atcacagccg tctattccga tgcagagaca
      541 gaaccatatc tgaaaaagca cgtctatcta ttcgggtttc agcaaaatca gccaaaagta
      601 ttggtgacac aacaaaatca ggggaatccc gataattatc tttacttcaa cgagacagcg
      661 aataatgaat tgaaaaatgg ctttaatcaa atcgtttatg gacaagcaaa ttaatgagat
      721 aaacaagggg ttgtgacatg tcttttatca caaccccttg ctcagtttat ttgttagtct
      781 tttat
//
LOCUS       DACKAC010000194          775 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.200, whole genome shotgun sequence.
ACCESSION   DACKAC010000194 DACKAC010000000
VERSION     DACKAC010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.200"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(248..772)
                     /locus_tag="I0B46_002867"
     CDS             complement(248..772)
                     /locus_tag="I0B46_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2085068.1"
                     /translation="MIYKIIEDDKQTELNMCYFDREQDFFYYGGMGGGSIVPKINLVE
                     LDVNNYRELYVFNSLDFEIPMKQMKNIDIHILPSESCQIEYSIEYFYKGKKIKKCDKC
                     FESAVVKFPQFKLNPAASIQFQGHVFDVLAKDNIKKYKKGNIFHIDIQISSNLEDIKK
                     AFKIKTIGTAPNFK"
BASE COUNT          262 a          107 c           87 g          319 t
ORIGIN      
        1 gacgctccct taacaataaa tgaagacttt tttattgtta attgtttatt tttctattat
       61 tagatatgaa aatcgcattt agataaaata gaaaaatatg aatatatttt aaatattaag
      121 atgagagtca ttaacttatg atagtagtca atttgctttt cactaattac aaaatatgtg
      181 atataggaag tttagttatt gttctcataa tgccatcctg tttttagatt atctagtaat
      241 ttttttgtta tttaaaatta ggagcagtgc caattgtttt aattttaaag gcttttttta
      301 tatcttctaa attagaagat atttgtatat caatatggaa tatattacct tttttgtatt
      361 tttttatgtt atctttagct aaaacatcaa atacatgtcc ttgaaattgt atcgaggcag
      421 ctggatttag tttaaattga ggaaacttta ccacagctga ttcaaaacac ttgtcacatt
      481 tttttatttt tttacctttg taaaaatatt cgattgaata ttctatttgg cacgactcac
      541 ttggcagtat atgtatgtca atatttttca tctgtttcat aggtatttca aaatctaaac
      601 tattaaaaac atataattcc ctgtaattat ttacatctaa ctctactaaa tttatctttg
      661 gaacaataga tcctccaccc attccaccat aataaaaaaa atcttgttct ctatcaaaat
      721 agcacatatt aagctcagtt tgtttatcat cctcaataat tttataaatc aaacc
//
LOCUS       DACKAC010000195          741 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.201, whole genome shotgun sequence.
ACCESSION   DACKAC010000195 DACKAC010000000
VERSION     DACKAC010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.201"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..>741
                     /locus_tag="I0B46_002868"
     rRNA            <1..>741
                     /locus_tag="I0B46_002868"
                     /product="23S ribosomal RNA"
BASE COUNT          226 a          151 c          220 g          144 t
ORIGIN      
        1 caaagagggt aaaaaccccg tagacgaaat gtggaagaca cctaggagga tcctgagtac
       61 ggcggaacac gagaaattcc gtcggaatcc gggaggacca tctcccaagg ctaaatactc
      121 cctagtgacc gatagtgaac cagtaccgtg agggaaaggt gaaaagcacc ccggaagggg
      181 agtgaaatag aacctgaaac cgtgtgccta caacaagtca aagcccgtta atgggtgatg
      241 gcgtgccttt tgtagaatga accggcgagt tacgattgca tgcgaggtta agttgaagag
      301 acggagccgc agcgaaagcg agtctgaata gggcgtttga gtatgtagtc gtagacccga
      361 aaccatgtga tctacccatg tccaggttga aggtgcggta aaacgcactg gaggaccgaa
      421 cccacgtacg ttgaaaagtg cggggatgag gtgtgggtag cggagaaatt ccaaacgaac
      481 ttggagatag ctggttctct ccgaaatagc tttagggcta gcctcggaat tgagaatgat
      541 ggaggtagag cactgtttgg actaggggcc catctcgggt taccgaattc agataaactc
      601 cgaatgccat tcattcatat ccgggagtca gactgtgagt gataagatcc atagtcgaaa
      661 gggaaacagc ccagaccacc agctaaggtc ccaaaatata tgttaagtgg aaaaggatgt
      721 ggggttgcac agacaactag g
//
LOCUS       DACKAC010000196          731 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.202, whole genome shotgun sequence.
ACCESSION   DACKAC010000196 DACKAC010000000
VERSION     DACKAC010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.202"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(138..491)
                     /gene="tnpB"
                     /locus_tag="I0B46_002869"
     CDS             complement(138..491)
                     /gene="tnpB"
                     /locus_tag="I0B46_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAR2085069.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(481..675)
                     /locus_tag="I0B46_002870"
     CDS             complement(481..675)
                     /locus_tag="I0B46_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2085070.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          220 a          156 c          122 g          233 t
ORIGIN      
        1 ttgaagcaac tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa
       61 ctgattttgt tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt
      121 tgtgttaacc ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga
      181 tcgatactta atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg
      241 tctttcgtcc gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa
      301 ccatcaccgt cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa
      361 acggcatctt cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca
      421 attccttttc gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa
      481 tcataaagca ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc
      541 gttatagatc aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc
      601 cgcgggttta cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaatctc
      661 atttaattta tacatagaaa acctccagac ttcttgatag ctcaagtata tcttgaggtg
      721 catttgttag t
//
LOCUS       DACKAC010000197          701 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.203, whole genome shotgun sequence.
ACCESSION   DACKAC010000197 DACKAC010000000
VERSION     DACKAC010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.203"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..>701)
                     /locus_tag="I0B46_002871"
     CDS             complement(<1..>701)
                     /locus_tag="I0B46_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2085071.1"
                     /translation="QNAKVLAKPAIPSQFSFSSLVDTLANVKIEKANFLEFFGGKEGS
                     ILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQSVLVGVYPFISS
                     SSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAAKDWLKTNIDKQ
                     KPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSARIVEYTFNVLT
                     NSYDKLVVGNVKTNF"
BASE COUNT          210 a          145 c          103 g          243 t
ORIGIN      
        1 aagaagtttg ttttgacgtt tcctacaact aatttatcgt atgaattggt tagaacatta
       61 aacgtatatt ccacaattct cgctgacata ttcactttta actgtggatg atacacatct
      121 actccgtcac ccatcgaaac tttttctaga tcaacaaatt tttcatagcc tctttgatgc
      181 ctcaatggta ctaattcaat cgaaccactc acttgtggtt tttgtttatc tatgtttgtt
      241 ttcaaccagt ctttagcagc ttcccttaat gtggctacat cagtcgcttt gtctttaaaa
      301 tcaacaaaag aaacatatcc agcaggataa tcatccacgt aatccgtgaa aataacttct
      361 tctggtagag tgatctcgtc ttctccttct gaagagctgc taatgaatgg ataaactcca
      421 actaaaacac tttgagcatc aatctctaag tcaagaccag ttaagttttt agtataaatc
      481 gctttgattt tatgatccgt acctagactt ttttcatgac gtaatgtgtt attatctttt
      541 agaaactcac catgaaatcg atctagaata gatccctctt ttccaccaaa gaattctaaa
      601 aaattcgcct tttctatctt cacattagca agcgtatcta ctaatgacga gaaagaaaac
      661 tgcgaaggga tagctggttt cgctaaaact tttgcgtttt g
//
LOCUS       DACKAC010000198          696 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.204, whole genome shotgun sequence.
ACCESSION   DACKAC010000198 DACKAC010000000
VERSION     DACKAC010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.204"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(53..628)
                     /locus_tag="I0B46_002872"
     CDS             complement(53..628)
                     /locus_tag="I0B46_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAR2085072.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
BASE COUNT          229 a          151 c          125 g          191 t
ORIGIN      
        1 atgaaaaaac agagccagat tagcattatt ttgctaatcc agctctgttt attcagccta
       61 tttttctttt tgaatatatc aagcgttggg gcaacatagt gatgactaat gaaacaacga
      121 cgatagacaa caagcgatca aggtagtctg tacccgcctg taccaagatc acacttgttg
      181 tctgacttaa acctaaccca tgtaatagct gaacgatcat actggatcct gaggaggtga
      241 tcccgccaaa taactgaacg gtaatgatcg aagaaacgat cgttccaggt accgacaaaa
      301 tcaaagcgag taataacagt ttcaagctgt ttttatcttt gccgagatat cttggcaaaa
      361 tcaatccagc aagcaagcct gtgagaattt ggattggcga ataatacaga gaaaaaatat
      421 ctgtagtcac tccacttaaa ataccgctgg acaatcctgt cagcattcca ggtacaggcc
      481 caagcaatgc accagcaaaa atcgttccga tagaatcgag atagattggc aaacgaagcc
      541 ataaagcaat cgttcctcca atataattga tcccaataca caaagccatc actgtaagta
      601 cgcgagttgc cattttgttt ttattcattt tgttagccca tctttaacct ttccaaatct
      661 ttcataatcg tttcttcttg tgcatttaaa agaacg
//
LOCUS       DACKAC010000199          688 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.205, whole genome shotgun sequence.
ACCESSION   DACKAC010000199 DACKAC010000000
VERSION     DACKAC010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.205"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..549
                     /gene="tnpB"
                     /locus_tag="I0B46_002873"
     CDS             <1..549
                     /gene="tnpB"
                     /locus_tag="I0B46_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAR2085073.1"
                     /translation="IDNNKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQ
                     KRKVARLHEKVMNQRTDFLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSW
                     SKFVTKLQYKADWYGRKIIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHD
                     RDINASINILTEGLRLHSMGLA"
BASE COUNT          256 a          110 c          131 g          191 t
ORIGIN      
        1 attgataata ataaattcac gtccaaaatg gaaaagaaac taaagcgtga acaacgcaaa
       61 ttgtctaggc gtgctttatt ggctaaaaat aaaggtacca atctatttga agctaaaaat
      121 tatcaaaaac aaaaacgcaa agtagctaga ttgcatgaaa aagtaatgaa tcaacgtact
      181 gattttctga ataagttgag tacagaaatg atcaaaaatc acgatattat ctgtattgaa
      241 gacttaaaca caaaaggtat gttgcgaaat cataaattag ctaaaagtat ttctgatgtt
      301 tcttggtcaa agtttgtgac taaattacaa tacaaggctg actggtatgg acgaaaaatc
      361 atcaaagtag ataaatggtt tccgtctagt cagatttgtt cagagtgcgg acataaagat
      421 ggcaagaaat ctcttgaaat tcgagaatgg acttgtcctg tttgtcatac tcatcatgat
      481 cgtgatatca atgctagtat caatattctg accgaaggtc taagactaca ttctatggga
      541 ttggcttaga tccaatcatc aagaaccgta ggaactacgg ggatagcttg gtaaataaga
      601 gacaccgctg ttagtaaaga aatacgctat caagtatgct ctattcccaa gaagctccca
      661 cttcaagcgt taagaaccgt caggttta
//
LOCUS       DACKAC010000200          684 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.206, whole genome shotgun sequence.
ACCESSION   DACKAC010000200 DACKAC010000000
VERSION     DACKAC010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.206"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..>684)
                     /locus_tag="I0B46_002874"
     CDS             complement(<1..>684)
                     /locus_tag="I0B46_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3-like element IS1476 family transposase"
                     /protein_id="HAR2085074.1"
                     /translation="DRRLYKLTQYFLRFPRKARLKVKYLVMDMNASYCQLLKTVFPNA
                     EIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYRRLKRYWKLLLKDSTTLEP
                     LKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLRYYFVKRGTEGFQETLKTI
                     SSTLPQSFKKKFTIFHRYQSGISNAFKVSHSNGVTEGLNNKIKLIKRIAFGYRNFYNF
                     RARIYLQQG"
BASE COUNT          191 a          123 c          155 g          215 t
ORIGIN      
        1 gaccttgttg taaatagatc cgtgcccgaa agttataaaa gttgcgataa ccaaacgcaa
       61 ttcgtttaat cagcttgatc ttgttattca gcccctcagt gacgccgttt gagtgggaca
      121 ccttaaaggc atttgaaata cccgattggt agcgatggaa gatcgtgaat ttctttttaa
      181 acgactgagg taacgtagat gaaattgttt taagggtttc ttgaaagcct tccgtacctc
      241 gtttaacgaa ataatagcgt aataattgac aaaaatgata cgcagttcgc aattcttcgt
      301 tgtagcttaa cagctcatca acgatttctg tttgactaat tggtcgttta aacagtcgat
      361 gataatgccg tttgagtggc tccaatgtcg tggaatcttt gagtaagagt ttccaatatc
      421 gcttgagccg acggtacttc ttctgatttt cagagtgtga ggttcgaaaa cgattcatga
      481 tctggacacg taattgattg aagctgcgat tgatgtgttg gacaatatga aagcggtcag
      541 ttacgatctc cgcatttgga aagactgttt taagcaactg acaatagctg gcattcatgt
      601 ccatcactag gtacttgacc tttaaacggg cctttctagg aaagcgcagg aagtattgag
      661 ttagtttgta aagtctgcga tctt
//
LOCUS       DACKAC010000201          655 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.207, whole genome shotgun sequence.
ACCESSION   DACKAC010000201 DACKAC010000000
VERSION     DACKAC010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.207"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            92..421
                     /locus_tag="I0B46_002875"
     CDS             92..421
                     /locus_tag="I0B46_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAR2085075.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            411..>655
                     /locus_tag="I0B46_002876"
     CDS             411..>655
                     /locus_tag="I0B46_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAR2085076.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCG"
BASE COUNT          251 a           73 c          126 g          205 t
ORIGIN      
        1 tacgagtcac agtaattact tgtaaataca ttatctcgtg gtataatgta tttactaagt
       61 aattacatac tgttagaaat gagggattta aatgattaca aaaacaagaa aacaaggcaa
      121 ttcaatcatg ttgacagtac ctaaagattt taatgttcct aatggagtcg aagttgaagc
      181 aaagttggta gagaatggta ttctttatga atttgttgaa ccacaaaaag aattttatga
      241 ttttagcgaa gatattttgt ctgatataat tgcagaaggc tacgataaag atgagattct
      301 tgtagaattt aagaatagaa aaaacaaaat gcattcttcc tttagagata ttgctgaaga
      361 tacacttact aattctaaag taatgactaa agaggagttg gcaacagaaa ttggcttatg
      421 aaatcgttcc ttcaaaacat gtcattaagt atttaaaaaa gctaaaagag aagccattga
      481 aagaaaaatt tttgaattta atttatgatg aaattgcgat tgatccatat aagggtgaac
      541 aaaaaactgg ggatttatca ggtatttggt ctagtgggtt caaatatgcg ggcacaacat
      601 atagagtagc ttacgagatt atagaaaaca aggttattcc agtattgtta tgtgg
//
LOCUS       DACKAC010000202          644 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.208, whole genome shotgun sequence.
ACCESSION   DACKAC010000202 DACKAC010000000
VERSION     DACKAC010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.208"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..210
                     /locus_tag="I0B46_002877"
     CDS             <1..210
                     /locus_tag="I0B46_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAR2085077.1"
                     /translation="KQAQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWD
                     RKSHELFISILKELEEHENWYAEVE"
     gene            211..>644
                     /locus_tag="I0B46_002878"
     CDS             211..>644
                     /locus_tag="I0B46_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2085078.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYP"
BASE COUNT          266 a           93 c          136 g          149 t
ORIGIN      
        1 aaacaagccc aagagattta tggggaacat ttgattaaaa atgttgtact caaaaatgcg
       61 cgcttaaaac gatacgacgg atcaggtatt acgatggaga agacaaagaa aggtctctta
      121 aaacaatggg atcgcaaaag ccacgaatta tttatcagta ttttgaaaga attggaagaa
      181 cacgagaatt ggtatgcgga ggtggagtaa ttggctgaca tcaaaggact tgatattttt
      241 gggaacaaca aaggactcgc caaaataaaa aaagaagcag aagcaaagaa aaagcagcaa
      301 gcaattacac catctgaaaa taagcaatta gaagtgcacc aagttcaaga aataaaaact
      361 tcctccaatg ttgaaagaca gagtagagga agaacaaaaa aagttggcgc accaatcaga
      421 aagtttgata aggtttattc caccaagcat cctttaaaac tgtcgacttt gttaaatgcg
      481 acttccagag ttttagtaga aaagtatgag actaatatga caagagatga attgcttaga
      541 aaagctttag atgagtatat taaacagaat cttacgcaag aagataagca agatttgttt
      601 aatgacgtgg tgaaagaact agacttgttc agagaaaaat atcc
//
LOCUS       DACKAC010000203          614 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.209, whole genome shotgun sequence.
ACCESSION   DACKAC010000203 DACKAC010000000
VERSION     DACKAC010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.209"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..308)
                     /locus_tag="I0B46_002879"
     CDS             complement(<1..308)
                     /locus_tag="I0B46_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAR2085079.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     "
BASE COUNT          195 a          119 c           86 g          214 t
ORIGIN      
        1 tctgctggaa gtatatagag gttattgatt actttataga cagcctcttt aggtggaaga
       61 ttgtttatca acacgtcagt cacagtattt tcgtaaacaa tcggttcacc ttctccgccc
      121 ccttcttcgt tagtgaagtt catatgcgtt aagttcatca cgtcagaagt atttccttgc
      181 atatccaagt caattatcaa ggttcttttt ccttcattaa ctgctgattc aaaccctaac
      241 atttgcgcgc acgtactttt acctacgcca cctttaaaat taccgattac atatttcttc
      301 gctaccacgt tcctacctct tttcttttag caatattcat ttgatactat tataacatat
      361 ttatttttag ttactaccaa aaaaaagata tatatgtact accttataat taataaggaa
      421 aaggtagtac ataaataagg aaaagctgaa atatattgat ttatagcttt cctcaaagta
      481 atgacgttat ttaatttatc gagaagcaga gaatactcgc gactttagtc atgtagacga
      541 atcagatttt cgataaaaaa gaagccgtcc ttcttaagaa cgacaacaaa tcactagatt
      601 aattttttca actg
//
LOCUS       DACKAC010000204          605 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.210, whole genome shotgun sequence.
ACCESSION   DACKAC010000204 DACKAC010000000
VERSION     DACKAC010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.210"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..>605
                     /locus_tag="I0B46_002880"
     CDS             <1..>605
                     /locus_tag="I0B46_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAR2085080.1"
                     /translation="MTKSFTEEVTSFKERELHDRYAAIYMDATYIPLKRKTVAKEAIH
                     IVVGIRPDGSKEVLSYAIAPTESITIWEEILIDLQERGVKNVLLFITDGLKGMAGAVQ
                     RFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMVYQASSKKAALEARGAFAEKW
                     KTSYPKMVESILSNDYLLTFYDFPLAIRKTIYSTNLIESFN"
BASE COUNT          173 a          129 c          137 g          166 t
ORIGIN      
        1 atgactaaat catttacgga agaagtcact tcatttaagg aacgtgagct tcatgaccgt
       61 tatgccgcta tttatatgga cgcaacctat atccctttaa agcggaaaac cgtcgccaag
      121 gaagccattc atatcgtagt tggcattcgc ccggacggtt caaaggaagt gttgagctat
      181 gcgattgcac caactgaatc tatcacgatt tgggaagaga ttttaataga cctccaggaa
      241 cgtggcgtga aaaacgtcct ccttttcatc acggatggct taaaggggat ggcaggggcc
      301 gtgcagcggt tctaccccaa agcccgtttt caacattgct gtgtgcacgt ttcccgtaat
      361 atcagtcaca aagtgcgtgt cgatgatcgt aaggaagtct gtgatgattt taaaatggtg
      421 tatcaagcgt catctaaaaa ggcggcattg gaagcacgtg gtgcttttgc ggaaaaatgg
      481 aaaaccagtt atccaaaaat ggtggaatcg atcctttcga acgattacct gctcactttc
      541 tatgatttcc cattagccat acgcaagact atttactcta cgaacttgat tgaatccttt
      601 aataa
//
LOCUS       DACKAC010000205          604 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.211, whole genome shotgun sequence.
ACCESSION   DACKAC010000205 DACKAC010000000
VERSION     DACKAC010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.211"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            35..>604
                     /locus_tag="I0B46_002881"
     CDS             35..>604
                     /locus_tag="I0B46_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAR2085081.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIIT"
BASE COUNT          207 a          112 c          115 g          170 t
ORIGIN      
        1 gttccgacca aagaattata aggagtgagt acaaatgacc tatacccatc ttacaacgga
       61 tgaacttgta ataatagagt cttatttcaa aatgaatcaa tctgttgcta aaactgccca
      121 ttgcttgaat cgttcaagac aaacgatcca taaagtatat ctgttcttca agcaaggaaa
      181 atcagcctta gaatattatc aacagtataa gaaaaacaaa tcaaactgtg gtagacgtcc
      241 gcttgtttta cccgaggaac aatcagaata tattcaaaga aaggttgttc aaggatggac
      301 acccgatgtg attgttggtc gtgcagcgtt tcctattagt tgttctgctc gtaccattta
      361 tcgtatgttc aaaaaggggc tatttgattc ttctgactta ccgatgaaag gcaagcgtaa
      421 accgaatgga catcaagaaa gacgtggaaa acaaactttc cgccgctcta ttcatgaacg
      481 tgaaaaggat tatagccaat tctcaaatga gtttggtcac cttgaaggtg acactatcgt
      541 aggtctgaaa cataaaagtg ctgtaattac cttagttgaa cgattatcaa aagttatcat
      601 caca
//
LOCUS       DACKAC010000206          604 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.212, whole genome shotgun sequence.
ACCESSION   DACKAC010000206 DACKAC010000000
VERSION     DACKAC010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.212"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..308)
                     /locus_tag="I0B46_002882"
     CDS             complement(<1..308)
                     /locus_tag="I0B46_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAR2085082.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     "
BASE COUNT          195 a          115 c           82 g          212 t
ORIGIN      
        1 tctgctggaa gtatatagag gttattgatt actttataga cagcctcttt aggtggaaga
       61 ttgtttatca acacgtcagt cacagtattt tcgtaaacaa tcggttcacc ttctccgcct
      121 ccttcttcgt tagtgaagtt catatgcgtt aagttcatca catcagaagt atttccttgc
      181 atatccaagt caattatcaa ggttcttttt ccttcattaa ctgctgattc aaaccctaac
      241 atttgcgcgc acgtactttt acctacgcca cctttaaaat taccaattac atatttcttc
      301 gctaccacgt tcctacctct tttcttttag caatattcat ttgatactat tataacatat
      361 ttatttttag ttactaccaa aaaaaagata tatatgtact accttataat taataagtaa
      421 aaggtagtac ataaataagg aaaagttgaa atatattgat ttatagcttt cctcaaagta
      481 atgacgttat ttaatttatc gagaagcaga gaatactcgc gactttagtc atgtagacga
      541 atcagatttt cgataaaaaa gaagccgtcc ttcttaagaa cgacaacaaa tcactagatt
      601 aatt
//
LOCUS       DACKAC010000207          601 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.213, whole genome shotgun sequence.
ACCESSION   DACKAC010000207 DACKAC010000000
VERSION     DACKAC010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.213"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(318..581)
                     /locus_tag="I0B46_002883"
     CDS             complement(318..581)
                     /locus_tag="I0B46_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circular bacteriocin, circularin A/uberolysin
                     family"
                     /protein_id="HAR2085083.1"
                     /translation="MKKNLLLVLPILGFAGFFVGVPMLSANIGISSYAAKKVIDIINT
                     GSTVATIISIVAAVVGGGLITAGIVATAKSLIKKYGAKYAAAW"
BASE COUNT          225 a          104 c           79 g          193 t
ORIGIN      
        1 gccttccttt tttgtatgga ttttttcatt tacacaaaat attttatact ctcctgaatt
       61 ttttcatgtt gaaaacatca ctaatcaaag gcaaattata atttagaaat agaatgaaaa
      121 cactaaatga aaatagaaca ttagaaaaaa tagaatcaaa aaaacccaag actagtgccg
      181 aaataaggga aacaatcgaa gctacaaaaa ataatgattt cgttgaacag gaccaattta
      241 tttgtttccg atatttctca aaaagaactt tctttgggaa agttaaaggc gactgtaaag
      301 tcgcctttag tcctttttta ccaagctgct gcatatttag ctccatattt tttaattaat
      361 gatttggctg tagcaacaat accagctgta attaatccgc ctccaacaac tgcagcaaca
      421 atagaaatga ttgtagcaac cgtgctacca gtattaatta tgtcaataac ttttttagct
      481 gcataagaag aaattccaat gttagcactt agcattggta caccaacaaa gaatcccgca
      541 aaacctaaaa ttggtaaaac taataataaa ttctttttca taataattcc tctatttttt
      601 t
//
LOCUS       DACKAC010000208          586 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.214, whole genome shotgun sequence.
ACCESSION   DACKAC010000208 DACKAC010000000
VERSION     DACKAC010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.214"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..221
                     /locus_tag="I0B46_002884"
     CDS             <1..221
                     /locus_tag="I0B46_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAR2085084.1"
                     /translation="NTGLTFSIENGADEVAALELETKVMQDEFGKFYHEAIVAELEED
                     LTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            241..>586
                     /locus_tag="I0B46_002885"
     CDS             241..>586
                     /locus_tag="I0B46_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2085085.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKE"
BASE COUNT          226 a           82 c          116 g          162 t
ORIGIN      
        1 cgaatacagg attaacattc tctattgaaa atggtgcgga tgaagtagct gcgttagaac
       61 tagaaacaaa agttatgcaa gatgaatttg gtaaatttta tcatgaagca attgttgcag
      121 aacttgaaga agacttaaca gatcaatgga tgacgaatct atctgctgat gtgattaaaa
      181 aaacttcagt tgtgacaact acggccactc aatcacagta aaaaaaacgg aggtagcgaa
      241 atgaacgaag attactcaaa aattgaacta aacgatggaa caattttgaa tttagaacct
      301 aaactgaata tcaagaaatt attgatgatc aatagagatt ttaacacaga cgagtttgca
      361 aaaatgactg tgggaaaagg atccatggat atttctgtta ttcaaggtgc aaaggctgtg
      421 tatattgctt accgccaagc gaacatgact gattatattt cattcgatga atttatcgat
      481 aaatgggatt ttgatatggc tactgccagc tatatttatc aattgatgat gttcaaacaa
      541 gcacgcgatg cttatcaaaa agaatttgaa aaagcaaata aggaaa
//
LOCUS       DACKAC010000209          575 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.215, whole genome shotgun sequence.
ACCESSION   DACKAC010000209 DACKAC010000000
VERSION     DACKAC010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.215"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            312..>575
                     /locus_tag="I0B46_002886"
     CDS             312..>575
                     /locus_tag="I0B46_002886"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAR2085086.1"
                     /translation="MSAVVTDLTYVRVGTNWAYICLMIDLFNREIIGHSCGNKKDAQL
                     VKRAIQSIPYSLQEIELFHTDRGKEFDNQTIQNLMNGLVIKFLS"
BASE COUNT          195 a          105 c           82 g          193 t
ORIGIN      
        1 ttttacactc tctattattc ttttcataaa tattgtaaat agaagtatta aaaatatata
       61 caaaaagtat tatttcgaga aagataaata ttttcaaaaa ccttttcata atcctcaccc
      121 tttcttacac tgataaatca tgcgtctaat attcaaatta tactcgatat gctttagcta
      181 atcagccaac tctgtttgtc tactatcatt atatcaatag acaatatctg ctcccgtctt
      241 tatgatcgtg ccaaagcaat caaaagaaaa ttgcgaatca cttagatcga aagtttactc
      301 aaacaaaagc attgtcagcg gttgtcacgg atttaactta tgttcgggtc gggacaaatt
      361 gggcttatat ttgtttgatg attgatttat tcaatcgtga aattattggc cattcttgcg
      421 gaaataaaaa agatgcgcag cttgtaaaaa gggccattca aagcattcct tactcacttc
      481 aagaaatcga actcttccat acggatcgtg gaaaagaatt tgataaccaa accattcaga
      541 atctcatgaa tggtttggtt atcaaattcc tttcc
//
LOCUS       DACKAC010000210          544 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.216, whole genome shotgun sequence.
ACCESSION   DACKAC010000210 DACKAC010000000
VERSION     DACKAC010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.216"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..395
                     /locus_tag="I0B46_002887"
     CDS             <1..395
                     /locus_tag="I0B46_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAR2085087.1"
                     /translation="KLPKKVSSDVKDELKSIFKASELELTRERKEHFLEKYGCDSKLS
                     AACDILENGFEDAIQILSFPENIRRRIRTTNVLERLNEEIRRRERVIRIFPNINSITR
                     IIGTLLMEKDTEWLASPRKYLEFNSNNI"
BASE COUNT          171 a           99 c          117 g          157 t
ORIGIN      
        1 ataagctgcc aaagaaagtt tcttctgatg taaaagacga gttgaagagt atctttaaag
       61 cctctgaact tgaattgaca cgtgaaagaa aagagcactt cttggaaaaa tatggttgtg
      121 attcaaaatt aagtgctgct tgcgacattt tagaaaacgg ctttgaagat gctattcaaa
      181 ttttgtcttt tcctgaaaat atccgccgaa ggattcgtac aaccaatgtg ttggaacggt
      241 tgaacgaaga gattcgccgg agagaacgag tgattcggat ttttccaaat atcaattcca
      301 tcactcggat aattggaaca cttttgatgg aaaaagacac tgagtggctg gcttctccac
      361 gaaaatattt agaatttaat tcgaataaca tttagcacca tgaacctgtg cgagtctctt
      421 ctttctgagg agagacatca agagtcgctg cgctactgct cctgacctct ctcctcagaa
      481 agaaaggcat ggcagttagg ttcacaacgc aaaacattgt gcttttgatg aggttgaact
      541 ttta
//
LOCUS       DACKAC010000211          487 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.217, whole genome shotgun sequence.
ACCESSION   DACKAC010000211 DACKAC010000000
VERSION     DACKAC010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.217"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..>487)
                     /locus_tag="I0B46_002888"
     CDS             complement(<1..>487)
                     /locus_tag="I0B46_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAR2085088.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IK"
BASE COUNT          142 a           92 c           86 g          167 t
ORIGIN      
        1 gttttatccg ttcattattg tagtaataga tatagctatc gatggctgat tttaattcct
       61 cgaaacagtt ataaactttc ccgtgaaaaa tctcttgttt taataagcca aaaaaattct
      121 ccataagtga attatctaag cagttccctt tccgagacat gctttgaaag attttgtact
      181 ccttcaattt gtttctataa gctttcattt gatacgccca accttgatct gagtgaaacg
      241 tacgacgata gggacaatcg ttagtggtct ggatagcttc ctccaaacct tccattaccg
      301 ctaatgcatt gggcttttca gagatacgat aggataaaat ctcactattg aacatatcca
      361 taaaaggatc tagatacaat ttcttttgac ggataatgcc tttggcatcc acttcatggt
      421 atttaaattc agttgtatcc gtcgtgattt tctgatggca gatactggtg tagaaacgac
      481 gatggat
//
LOCUS       DACKAC010000212          481 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.218, whole genome shotgun sequence.
ACCESSION   DACKAC010000212 DACKAC010000000
VERSION     DACKAC010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.218"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..143)
                     /locus_tag="I0B46_002889"
     CDS             complement(<1..143)
                     /locus_tag="I0B46_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAR2085089.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQ"
     gene            complement(154..360)
                     /locus_tag="I0B46_002890"
     CDS             complement(154..360)
                     /locus_tag="I0B46_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAR2085090.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT          137 a          107 c           66 g          171 t
ORIGIN      
        1 tactgattat ttgctacttg tgagctacct tcatttgcgc ccaaattaaa ttcattgttg
       61 atagtatagg caaatccgtt aattggcaat aaaaaaagag ccgttaacag gctcatcgca
      121 gtaatagtaa ttttcttttt catatctatc ctcctattta tctgatgaaa ttttcttata
      181 cgttcgattt gatacaccta aaatactccc tagaaatgct ccaaaacctg taataatcat
      241 caccgttata tctgtgtacc gccagttaac accttttccg acaagaccga caaaagtcgc
      301 taaagcgggg ataactacta tagcgatcca tttcaaaata tcaaaggttc tattgttcat
      361 actatccacc tcccttcatt ggttgggcta ctttcttttt caatcgcttg ttttcttcta
      421 tcaatatttc tctttctgaa agcaaggcat caatttgccc gtccagttgt tcaattttat
      481 c
//
LOCUS       DACKAC010000213          478 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.219, whole genome shotgun sequence.
ACCESSION   DACKAC010000213 DACKAC010000000
VERSION     DACKAC010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..478
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.219"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..270
                     /locus_tag="I0B46_002891"
     CDS             <1..270
                     /locus_tag="I0B46_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAR2085091.1"
                     /translation="ELVKLEMAETGAGVRSSRHWKAAANIEFYYNEIQKGFDQMRELD
                     RQTNWSKKLHQDRFKFVEKYKEILEEYLRRTANDKKTRSIQHGFI"
     gene            230..475
                     /locus_tag="I0B46_002892"
     CDS             230..475
                     /locus_tag="I0B46_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2085092.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
BASE COUNT          181 a           58 c          116 g          123 t
ORIGIN      
        1 gaattagtta agttagaaat ggctgaaaca ggagcaggag tgcgaagtag caggcattgg
       61 aaagcagcag caaacattga attttattac aacgaaattc aaaaagggtt cgatcagatg
      121 cgtgagctgg atcggcaaac aaattggagc aagaaacttc atcaagatcg tttcaaattt
      181 gtagagaagt ataaagaaat attagaagag tatttgagga ggacagcaaa tgataaaaaa
      241 actcgttcaa ttcagcatgg atttatatga tatcgaatca ggagcaacac tatctgtgga
      301 atcggaccat ctaatcataa attttggtgg aaagcgccag attattttgt gggtagttga
      361 tgatgtactg tttccagaaa ttgttcatga tttcgaagaa tcaaaagcgg ttgagtttga
      421 aatagtgaaa aaagtaatgg aattgattga aaaatacgag gaggacagcg aatgatac
//
LOCUS       DACKAC010000214          471 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.220, whole genome shotgun sequence.
ACCESSION   DACKAC010000214 DACKAC010000000
VERSION     DACKAC010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.220"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            77..>471
                     /locus_tag="I0B46_002893"
     CDS             77..>471
                     /locus_tag="I0B46_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAR2085093.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTP"
BASE COUNT          179 a           87 c           76 g          129 t
ORIGIN      
        1 aatccataca aaaaaggaag tcccttctgt agaataaagt taacgacaac caattcacag
       61 aaaagaggac ttccctatga atgattttac tacagaaatt gtgcaaactc tagtcactaa
      121 aggcgattta aatgaattat tccgttcgca cttagaaaaa gcgataaaca cactcctacg
      181 gactgaatta acggcttttt tagattacga aaaatatgat cgcactggtt ttaattcagg
      241 taattcgaga aacggttctt actttcgatc aatcaaaacc gaatatggtg aattaacatt
      301 ggaaatacct agagatcgta atggtgagtt taaacaacaa actttaccag cctacaaaag
      361 aacaaacgat acattggaaa ccactattat ccatttattc gaaaaaggtg ttacgatgtc
      421 tgaaattgct gatttgatcg aaaaaatgta cggtcatcac tatactccac a
//
LOCUS       DACKAC010000215          462 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.221, whole genome shotgun sequence.
ACCESSION   DACKAC010000215 DACKAC010000000
VERSION     DACKAC010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.221"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..430)
                     /locus_tag="I0B46_002894"
     CDS             complement(<1..430)
                     /locus_tag="I0B46_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAR2085094.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          132 a           92 c           59 g          179 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tc
//
LOCUS       DACKAC010000216          448 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.222, whole genome shotgun sequence.
ACCESSION   DACKAC010000216 DACKAC010000000
VERSION     DACKAC010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.222"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
BASE COUNT          142 a           90 c           68 g          148 t
ORIGIN      
        1 gacactcctc ttaatataca gtcctattgt ataaagtgtt actatataca tagtaactgt
       61 atacataaaa aaagtcaagt attagaaaaa agttcaaaag atcaagtgtg cgcttcgctc
      121 ccactcccct cgacgtttta gtagcctttc cctcacttcg ttcagtccaa gccaactaaa
      181 aagttttcgg gatacattct ccttctcttc ctaataatca attaaaattt taatctttat
      241 atttctgcta atcactcagg aaacagtaca gaaaaacaaa cacgtatcat agatataaat
      301 gtaatggcat agtgcgggtt ttattttcag cctgtatcat agctaaacaa atcgagttgt
      361 gtgtccgttt ttgggcgttc tgctagcttg tttaaagtct cttgaatgaa tgtatgttct
      421 aagtcaaaaa actctgacag cgccttta
//
LOCUS       DACKAC010000217          440 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.223, whole genome shotgun sequence.
ACCESSION   DACKAC010000217 DACKAC010000000
VERSION     DACKAC010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.223"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            44..>440
                     /locus_tag="I0B46_002895"
     CDS             44..>440
                     /locus_tag="I0B46_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAR2085095.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEI"
BASE COUNT          137 a           83 c           86 g          134 t
ORIGIN      
        1 cctttagaat taatttggct accaattcat aggaggattc aagatgtctc atactcagct
       61 tatcaaagat accctaaata ttctagacct aaatatctat ttcgaggaaa attgtttaac
      121 taaagaaaaa tacaaagggc aaatttgctt gatttacaaa ggaactcttc tgtacaagcc
      181 agaagagtgt tcccattgtc tttgtgtcgt cccctctagg atcattcgtt gggggacgac
      241 aaccgttcgg ttgttactga atgatgtgtc tgagtatcgc acttatctgg agctgaagaa
      301 acaacgattt aaatgtaagt cttgccaaag aacattcgta gcggatacct ctgttgctga
      361 aaaacactgt ttcattagtc aaaaagttcg ttggtctgtc attgctcgct taaaagagaa
      421 tacttccatg acggaaatcg
//
LOCUS       DACKAC010000218          415 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.224, whole genome shotgun sequence.
ACCESSION   DACKAC010000218 DACKAC010000000
VERSION     DACKAC010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.224"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..339)
                     /locus_tag="I0B46_002896"
     CDS             complement(<1..339)
                     /locus_tag="I0B46_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAR2085096.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DACKAC010000219          413 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.225, whole genome shotgun sequence.
ACCESSION   DACKAC010000219 DACKAC010000000
VERSION     DACKAC010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.225"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..390
                     /locus_tag="I0B46_002897"
     CDS             <1..390
                     /locus_tag="I0B46_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAR2085097.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          161 a           74 c           65 g          113 t
ORIGIN      
        1 aaagaccgaa aagaaatctg tgatgacttt aaggctgttt atcaagctaa ctcaaaagaa
       61 gaagcgaata ccttcttatc cggcatgatt gagaaatgga agaaaaacta tcctaaagtg
      121 acgcagtcac tcatagaaaa ccaagactta ttaacttttt atgattttcc acctagcatt
      181 cgtagaacca tttactcaac caatctaatc gagtctttca ataagcaaat taaaagatac
      241 agccgtagaa aagagcagtt tcaaaatgaa gaatcactag aacgctttct agtcagcatt
      301 tttgatacat acaatcaaaa atttctaaac agaagccata aaggttttca acaggtaacc
      361 gatacattag tttcaatgtt tactgagtaa ctaattattt tgcaggagga caa
//
LOCUS       DACKAC010000220          406 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.226, whole genome shotgun sequence.
ACCESSION   DACKAC010000220 DACKAC010000000
VERSION     DACKAC010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.226"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            16..>406
                     /locus_tag="I0B46_002898"
     CDS             16..>406
                     /locus_tag="I0B46_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2085098.1"
                     /translation="MKPTEGLANTWELTFTVTKQHIDSGVTNYLEIYQYPSATKGAVQ
                     IEWLKLEKGNTRTPNISEYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEIT
                     GKANNYTDGKVSEINSQLTASINEVDTT"
BASE COUNT          149 a           64 c           86 g          107 t
ORIGIN      
        1 gttaaagtag gagacatgaa gcctactgag gggttagcta atacatggga gttaactttt
       61 actgtaacta agcaacatat agatagtggg gtaactaatt atctggaaat ataccaatac
      121 ccgtcagcta caaaaggagc tgttcaaatt gagtggctca aactagaaaa aggcaatact
      181 cgaaccccga atattagtga gtataaatat cgtggtactg gtatgcgtga ttcaaacaat
      241 ccaaaagatt atgtttggga tctagcacca gaatatgtcg aagataattt ggccacagat
      301 attaaaattt ctgaaattac tggtaaagca aacaattata ccgatgggaa agtatcggag
      361 attaattcgc agttgactgc ttcaattaat gaagtagaca ccacag
//
LOCUS       DACKAC010000221          378 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.227, whole genome shotgun sequence.
ACCESSION   DACKAC010000221 DACKAC010000000
VERSION     DACKAC010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.227"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..>378
                     /locus_tag="I0B46_002899"
     CDS             <1..>378
                     /locus_tag="I0B46_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2085099.1"
                     /translation="RMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYIATWT
                     KNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQEHKNT
                     VETAEQKNIVPVGSLWIDKKDGKV"
BASE COUNT          142 a           66 c           90 g           80 t
ORIGIN      
        1 agaatgcaca aattttcaga attagatact gcaacaaaag aactacaggc agaaatgacc
       61 tcctatattg tgtgcaagca ttttgggatg gacacctcgg agaaagctat tccttatatt
      121 gcgacatgga cgaagaatgg tcagacgttg gatgataagg aagctgcgga acgtgggaaa
      181 atcatgaatg atgtcagtcg tgtcgcaaat gaattcattc aaaccatttc taatgaaatc
      241 aatcaataca gagaaagaga accagagatc cagccagcag aagtgaagca ggagcataaa
      301 aatactgtag aaacggcaga acaaaagaat attgtgcccg ttggctcact ttggatcgac
      361 aagaaagacg ggaaagtc
//
LOCUS       DACKAC010000222          376 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.228, whole genome shotgun sequence.
ACCESSION   DACKAC010000222 DACKAC010000000
VERSION     DACKAC010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.228"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            82..>376
                     /locus_tag="I0B46_002900"
     CDS             82..>376
                     /locus_tag="I0B46_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAR2085100.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTH"
BASE COUNT          133 a           62 c           76 g          105 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcgaa cccatt
//
LOCUS       DACKAC010000223          375 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.229, whole genome shotgun sequence.
ACCESSION   DACKAC010000223 DACKAC010000000
VERSION     DACKAC010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.229"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            7..375
                     /locus_tag="I0B46_002901"
                     /pseudo
     CDS             7..375
                     /locus_tag="I0B46_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          146 a           64 c           68 g           97 t
ORIGIN      
        1 atttttatgt ctaagagaac acgaagaact ttttcacaag aattcaagca acaaatcgtc
       61 aatctttact tagctggaaa gccacgtgta gaaatcattc gagaatatga actaacggct
      121 tcagcatttg acaaatgggt aaagcaatct aaaacgagtg gttcattcaa agaaaaagat
      181 aatcttacgc ctgaacaaaa agaattgtta gaactacgta aaagaaacca gcaattagaa
      241 atggaaaatg atattttaaa gcaagcagcg ctgatattcg gacgaagaga caagtaatcg
      301 atgcgaataa gcatctttac cctatatcag cgatgtgcag aatattaggt ctatcacgtc
      361 agtcctatta ttatc
//
LOCUS       DACKAC010000224          375 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.230, whole genome shotgun sequence.
ACCESSION   DACKAC010000224 DACKAC010000000
VERSION     DACKAC010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.230"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..132)
                     /locus_tag="I0B46_002902"
     CDS             complement(<1..132)
                     /locus_tag="I0B46_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAR2085101.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          132 a           69 c           76 g           98 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaat
//
LOCUS       DACKAC010000225          374 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.231, whole genome shotgun sequence.
ACCESSION   DACKAC010000225 DACKAC010000000
VERSION     DACKAC010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.231"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..>374)
                     /locus_tag="I0B46_002903"
     CDS             complement(<1..>374)
                     /locus_tag="I0B46_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2085102.1"
                     /translation="SNLINDSIDQLKNGGEISDLINDIVDHQTDMITGQNGGYVLLDP
                     KEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGINGTYETAWTLDGGFNASFITAG
                     EIVGITIRGTTLISDGADYRTS"
BASE COUNT          120 a           81 c           50 g          123 t
ORIGIN      
        1 atacttgttc tataatcagc accatcacta attaatgtag tacctctaat agtaatccct
       61 actatctcac cagctgtaat aaacgaggca ttgaatccgc catctaacgt ccatgcggtt
      121 tcatatgttc cattaatacc agttttagaa aaaccaatac cagcgttgtt gatttgtaaa
      181 acattccgtg cggtattctt atctggcgtg tccataatca aaatacgact aggcgcttct
      241 ttaggatcta ataaaacata accaccattt tgaccagtaa tcatatcagt ttgatgatct
      301 acaatatcat tgattaaatc actgatttcg ccaccatttt tcaattgatc aatggaatca
      361 ttaatcaaat tgct
//
LOCUS       DACKAC010000226          374 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.232, whole genome shotgun sequence.
ACCESSION   DACKAC010000226 DACKAC010000000
VERSION     DACKAC010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.232"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..>374)
                     /locus_tag="I0B46_002904"
     CDS             complement(<1..>374)
                     /locus_tag="I0B46_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2085103.1"
                     /translation="SNLINDAIDQLKNGGEISDLLNDIVDHQTDMITGQNGGYVLLDP
                     KEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGINGTYETAWTLDGGFNASFITAG
                     EIVGITIRGTTLISDGADYRTS"
BASE COUNT          118 a           81 c           50 g          125 t
ORIGIN      
        1 atacttgttc tataatcagc accatcacta attaatgtag tacctctaat agtaattcct
       61 actatttcac cagccgtaat aaacgaggca ttgaatccgc catctaacgt ccatgccgtt
      121 tcatatgttc cattaatgcc agttttagaa aaaccaatac cagcattgtt gatttgtaaa
      181 acattccttg cggtattctt atctggtgtg tccataatca aaatacgact aggcgcttct
      241 ttaggatcta ataaaacata accaccattt tgaccagtaa tcatatcagt ttgatgatct
      301 acaatatcat tgagtaaatc actgatttcg ccaccgtttt tcaattgatc aatggcatca
      361 ttaatcaaat tgct
//
LOCUS       DACKAC010000227          361 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.233, whole genome shotgun sequence.
ACCESSION   DACKAC010000227 DACKAC010000000
VERSION     DACKAC010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.233"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
BASE COUNT          123 a           39 c           58 g          141 t
ORIGIN      
        1 taggactcaa ttgttcatag atgagcattt gtaattaagt tatattataa taaaaaaata
       61 cgaattttag tgacgccatt atattgtttt tatttttcaa ttgtaatatt tcatattact
      121 tccataataa ggagtatttt ctattggaat atcttagctc ttttaaggat ttctcctttt
      181 ttgtttctca ttttttaaga ttatttggat ttcctttaca acaaacttcc acaagtatta
      241 ttgacataga tgtaattgag ggaagggggt tccagtgcta gaataaataa tgaaaagagg
      301 taacaaatgc tagcgttaag aggtgaacaa gtttgattga aagacatgta aatttattaa
      361 a
//
LOCUS       DACKAC010000228          352 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.234, whole genome shotgun sequence.
ACCESSION   DACKAC010000228 DACKAC010000000
VERSION     DACKAC010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.234"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
BASE COUNT          158 a           33 c           58 g          103 t
ORIGIN      
        1 atttataaat atcattcact catgataccc agctccgtat cacaaataat gatatttttt
       61 aagagaaaaa attatcccta ttttaaataa tagcaatagg tagtggtaga gagaaattta
      121 taaataactt tgttatttgc tttggtagcc aagtaagttg caaaagaaaa atagatactg
      181 ttaaaaagat aatgtagaca tagaaacaaa tacgtaaaat tttagttaga atgatggcaa
      241 ggagaaaaaa catgatagat gaaaaaaagt gtttaaaaaa tctattggaa gatatggatg
      301 atattgaaga aaaactatct aaatggactg ggaaaacaaa tatatttcag aa
//
LOCUS       DACKAC010000229          349 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.235, whole genome shotgun sequence.
ACCESSION   DACKAC010000229 DACKAC010000000
VERSION     DACKAC010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.235"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(44..>349)
                     /locus_tag="I0B46_002905"
     CDS             complement(44..>349)
                     /locus_tag="I0B46_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAR2085104.1"
                     /translation="TIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQF
                     ETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT           98 a           86 c           64 g          101 t
ORIGIN      
        1 atcctcggat ctgattttct ccggcagaag cacaatcatt caattacgct gcctctccag
       61 aggtatcaga tccgcgctca ttatcaagga ttcgcttcgc caatctctgt tgtcgaattt
      121 ggatcggtgt ttcatacgcc aacgtgccat gtaagcgtag atagttccac caatgcacat
      181 aatcaaacaa ttctagacgt agctgtgcca gtgtctcaaa ttggtattga tgcacgaatt
      241 ctactttgac agatttatag gttgattcca caacggcatt atcatacgga caacccttcc
      301 tactcaatga acgagtaatt ccaaaaccat tcagaatctc atgaatggt
//
LOCUS       DACKAC010000230          344 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.236, whole genome shotgun sequence.
ACCESSION   DACKAC010000230 DACKAC010000000
VERSION     DACKAC010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.236"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..>344
                     /locus_tag="I0B46_002906"
     CDS             <1..>344
                     /locus_tag="I0B46_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAR2085105.1"
                     /translation="TTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEMQTVVKKCG
                     ATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSVGEDFSLAA
                     EIVDDFEENRKL"
BASE COUNT          128 a           57 c           74 g           85 t
ORIGIN      
        1 taacaacgtt cgataacgta agtatcaaac gaattgcttt taattttaag aacgcaggaa
       61 atgcaatcgc aacgaattgt aacggacaat tagatggcga aacagaaatg caaacggtgg
      121 ttaaaaaatg tggagcgaca gaagtaaaat caaaatctaa accaatcaat atgacggtaa
      181 caattactgc acatgtaccg atggaagttt atcgacgttt caatgggttg aaacaagatg
      241 gacgtattaa accaggcatt tactcttacg gtcctgattc cgtaggcgaa gatttctcac
      301 ttgctgcaga gatcgtggat gacttcgaag aaaatagaaa gtta
//
LOCUS       DACKAC010000231          324 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.237, whole genome shotgun sequence.
ACCESSION   DACKAC010000231 DACKAC010000000
VERSION     DACKAC010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.237"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..>324
                     /locus_tag="I0B46_002907"
     CDS             <1..>324
                     /locus_tag="I0B46_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAR2085106.1"
                     /translation="KQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFI
                     GTMKKEFPIPISWTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEG
                     FSHPEM"
BASE COUNT           84 a           67 c           85 g           88 t
ORIGIN      
        1 aaacaatacc aagcagattt agcagggatt gggattggct catttgggcc tatcgatatc
       61 catcgcgatt ccgccactta tgggtacatt acctcgaccc cgaaattggc atggcaaaat
      121 tttgatttca tcggtaccat gaaaaaagaa tttccgatcc cgatttcttg gacaacagat
      181 gtcaatgcgg ctgcctatgg tgagtatgtc ttcggcagtg gcaaaggatt gagcagtgtc
      241 gtttactata cgatcggtac gggtgttggc ggcggtgcct tacaagaagg gcgctttatc
      301 gaagggttta gtcatccaga aatg
//
LOCUS       DACKAC010000232          323 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.238, whole genome shotgun sequence.
ACCESSION   DACKAC010000232 DACKAC010000000
VERSION     DACKAC010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.238"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..>323
                     /locus_tag="I0B46_002908"
     CDS             <1..>323
                     /locus_tag="I0B46_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAR2085107.1"
                     /translation="FRVHLEKAVNTLLKTELTAFLDYEKYDRIGFNTGNSRNGSYDRT
                     VKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEETVIHLFRKGITMSEIADLIEK
                     MYGHH"
BASE COUNT           91 a           78 c           74 g           80 t
ORIGIN      
        1 ttccgtgtcc atttggaaaa agctgtcaat acgcttctca aaacggagtt aacggctttc
       61 ctcgattacg aaaagtacga tcgcattggt tttaacacgg gtaattctcg taacggctcc
      121 tatgaccgta cggtcaagac cgagtacggg gaacttcatc tccagattcc gcgcgaccgc
      181 aacggcgagt tcaagcaaca gactgttcct gcttatagac ggacgaatga taccttagag
      241 gagaccgtca ttcacctctt ccgaaaaggt attaccatgt cggaaatcgc agacttgatt
      301 gagaaaatgt atgggcatca cta
//
LOCUS       DACKAC010000233          305 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.239, whole genome shotgun sequence.
ACCESSION   DACKAC010000233 DACKAC010000000
VERSION     DACKAC010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.239"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..302)
                     /locus_tag="I0B46_002909"
     CDS             complement(<1..302)
                     /locus_tag="I0B46_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HAR2085108.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT           95 a           45 c           50 g          115 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgtt
//
LOCUS       DACKAC010000234          301 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.240, whole genome shotgun sequence.
ACCESSION   DACKAC010000234 DACKAC010000000
VERSION     DACKAC010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.240"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..>301
                     /locus_tag="I0B46_002910"
     CDS             <1..>301
                     /locus_tag="I0B46_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2085109.1"
                     /translation="ANNLAEHLYDTQGLGALSDWLTATVSNKLNGAEIFQGTYSINGT
                     NADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPIFNDIRKSVLN"
BASE COUNT          110 a           42 c           62 g           87 t
ORIGIN      
        1 acgcgaataa tttagctgaa catttatatg atacgcaagg tttaggcgct ttgtcagact
       61 ggttaacagc tactgttagc aataaactaa acggagccga gatatttcag ggtacttatt
      121 caataaacgg aactaatgca gatttgattg tagaaggacg tattattcag tgttatgtag
      181 atgaaaatcg agcaaaacag cgtctacgga tttattatgc aaaaacttct gtaataggca
      241 atacgataga agtaaaagct gaacctattt tcaatgatat aagaaaatcg gtgttgaata
      301 a
//
LOCUS       DACKAC010000235          301 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.241, whole genome shotgun sequence.
ACCESSION   DACKAC010000235 DACKAC010000000
VERSION     DACKAC010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.241"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..>301
                     /locus_tag="I0B46_002911"
     rRNA            <1..>301
                     /locus_tag="I0B46_002911"
                     /product="23S ribosomal RNA"
BASE COUNT           93 a           59 c           87 g           62 t
ORIGIN      
        1 ataagggcgc acggtggatg ccttggcact aggagccgat gaaggacggg actaacaccg
       61 atatgctttg gggagctgta cgtaagctat gatccagaga tttccgaatg ggggaaccca
      121 acatctttaa taggatgtta cgattgtgtg aatacatagc acattcgagg tagacgcaga
      181 gaactgaaac atctaagtac ctgcaggaag agaaagaaaa ttcgattccc tgagtagcgg
      241 cgagcgaaac gggaaaagcc caaaccagca agcttgcttg ttggggttgt aggactccaa
      301 t
//
LOCUS       DACKAC010000236          296 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.242, whole genome shotgun sequence.
ACCESSION   DACKAC010000236 DACKAC010000000
VERSION     DACKAC010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.242"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..>296)
                     /locus_tag="I0B46_002912"
     CDS             complement(<1..>296)
                     /locus_tag="I0B46_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAR2085110.1"
                     /translation="DWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSK
                     SAMYIADWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESG"
BASE COUNT           99 a           57 c           46 g           94 t
ORIGIN      
        1 tcaccgcttt ccaacatgaa aggaataaca ccagcaacat ataaatttgt atcaaattct
       61 ttcacaaaaa agtccatata ctcaagatat tgcatcgcat ttctcatcga ttttacttgg
      121 gtctgtaaga cgacgattgc ccagtccgca atgtacatcg cggatttact atatacagaa
      181 atagaaggtg ggacatctaa atagatgacg tcaaactcat caaataatgg ttctagcttt
      241 tctttcatat atttgaattg ggataatgaa tcattatatt ggtctttgat ccaatc
//
LOCUS       DACKAC010000237          264 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.243, whole genome shotgun sequence.
ACCESSION   DACKAC010000237 DACKAC010000000
VERSION     DACKAC010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.243"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            83..>264
                     /locus_tag="I0B46_002913"
     CDS             83..>264
                     /locus_tag="I0B46_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAR2085111.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
BASE COUNT           83 a           55 c           43 g           83 t
ORIGIN      
        1 cctatgaaat attttcacgt gttaaagtat tcctcgttga acacaacaaa taaaactttt
       61 taacgaataa aggagatttt tcatgacatc acttaaaact ttactttttg gaacaacttt
      121 ggctgccggc gctgcattct tcatgggaac gactgctcat gcggacgaag cttataccgt
      181 tcaatcaggt gatactctat caacgatttc tcaaaaatac gttggtgata actccttgat
      241 caatgccatt gcagaatcaa attc
//
LOCUS       DACKAC010000238          260 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.244, whole genome shotgun sequence.
ACCESSION   DACKAC010000238 DACKAC010000000
VERSION     DACKAC010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.244"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..>260)
                     /locus_tag="I0B46_002914"
     CDS             complement(<1..>260)
                     /locus_tag="I0B46_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAR2085112.1"
                     /translation="NQWFESVPKNLFKSITFDCGKEFSNWKQISNVNDIAIYFADPGT
                     PSQRGLNENSNGLLRRDGLLKSMDFNSVDEFFIQSVASKRNN"
BASE COUNT           85 a           46 c           41 g           88 t
ORIGIN      
        1 attatttcgt ttagatgcga cagattgaat aaaaaattca tctactgaat tgaaatccat
       61 agatttcaat aaaccatctc tacgtaacaa tccgttagaa ttctcgttta ggcctctttg
      121 agacggcgtt cctggatcag cgaaataaat ggcaatatca ttgacattac tgatctgttt
      181 ccaatttgaa aattcctttc cacaatcaaa agtgatggat ttgaataggt ttttcggtac
      241 agattcaaac cattgattta
//
LOCUS       DACKAC010000239          258 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.245, whole genome shotgun sequence.
ACCESSION   DACKAC010000239 DACKAC010000000
VERSION     DACKAC010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.245"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            1..258
                     /locus_tag="I0B46_002915"
                     /pseudo
     CDS             1..258
                     /locus_tag="I0B46_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          103 a           49 c           52 g           54 t
ORIGIN      
        1 ctcgcgcaga aattattcga gagtatgagc ttacgccttc ttctttcgat aaatggatga
       61 agcaagcaca atcaacgggc tcattcaaag aaagagacaa cttaacacca gaacaagcag
      121 aattgatcgc actaagaaag aaaaataagc aactcgaaat ggagaatgat attttaaagc
      181 aagcggcgct gatattcgga cgaaaagaca agtaattgat gccaacaagc ataaatattc
      241 catatcagcg atgtgcaa
//
LOCUS       DACKAC010000240          253 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.246, whole genome shotgun sequence.
ACCESSION   DACKAC010000240 DACKAC010000000
VERSION     DACKAC010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.246"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..161
                     /locus_tag="I0B46_002916"
     CDS             <1..161
                     /locus_tag="I0B46_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HAR2085113.1"
                     /translation="IKNHEKFLIRNYKISGGGCYLLECRFTSKKSMNSFLEQLNEYAN
                     YKLSIVID"
BASE COUNT          105 a           28 c           37 g           83 t
ORIGIN      
        1 ttattaaaaa tcatgaaaaa ttcttgatta gaaattataa gattagtgga gggggctgtt
       61 atcttttgga atgtagattt acttcaaaaa aatcaatgaa cagctttttg gaacagctta
      121 atgaatatgc gaattataaa ttatctattg ttatagatta ataataaaac tagcagatgg
      181 taaaaatact aatatgcgag acacacattc aaccataact atataactta tcaatttaaa
      241 actaaactga tgg
//
LOCUS       DACKAC010000241          250 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.247, whole genome shotgun sequence.
ACCESSION   DACKAC010000241 DACKAC010000000
VERSION     DACKAC010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.247"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..>250
                     /locus_tag="I0B46_002917"
     CDS             <1..>250
                     /locus_tag="I0B46_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAR2085114.1"
                     /translation="SGYYVKGEREKQFAYSAHTSCDDNGFILSTIITPGNIHDSQVAF
                     QLVKQSKRLFPEINCVVADAGYKTPKFVHFLTHLNLRPC"
BASE COUNT           70 a           57 c           45 g           78 t
ORIGIN      
        1 agcggctact acgtcaaagg cgaacgagaa aaacaatttg cttattctgc ccatacttca
       61 tgcgacgaca atggatttat tcttagcaca atcatcactc ctggaaatat tcatgacagt
      121 caagtcgcct ttcagctcgt caaacaatcg aagagacttt ttcctgaaat caattgtgtt
      181 gtcgctgatg ctgggtataa gacacctaag tttgttcact ttttgactca tttgaacctt
      241 cgaccttgtt
//
LOCUS       DACKAC010000242          248 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.248, whole genome shotgun sequence.
ACCESSION   DACKAC010000242 DACKAC010000000
VERSION     DACKAC010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.248"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..>248
                     /locus_tag="I0B46_002918"
     CDS             <1..>248
                     /locus_tag="I0B46_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAR2085115.1"
                     /translation="LSSNLAYTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQ
                     AKLLNQIAKVGYNSTKKSYFYGLKIHMIVTKTGFPITY"
BASE COUNT           90 a           44 c           34 g           80 t
ORIGIN      
        1 gcttaagttc aaacttagct tacactatca agattattcg atactttttc atcaagaagc
       61 tcaccaaagg tgaactagtc ggaattatag atagttttcc tagtccatta tgtaaaccag
      121 ttagaaatag acaagcaaaa ctattgaatc aaatagccaa agttggctat aactcaacaa
      181 aaaaatctta tttttatggt cttaaaattc atatgatcgt tactaagact ggctttccga
      241 ttacttac
//
LOCUS       DACKAC010000243          243 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.249, whole genome shotgun sequence.
ACCESSION   DACKAC010000243 DACKAC010000000
VERSION     DACKAC010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..243
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.249"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..>243)
                     /locus_tag="I0B46_002919"
     CDS             complement(<1..>243)
                     /locus_tag="I0B46_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAR2085116.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMA"
BASE COUNT           71 a           45 c           48 g           79 t
ORIGIN      
        1 aggccatgta tttctcttct ttgatagggg aagttgtgaa tcgttgaata cgcaagaaat
       61 cagcgattct taatgcatcg tttcgatcag ttttattctc atcaaacatg cgactaaatt
      121 gtttcacccg aaaaggattt tcaatcgtta caagtgtgtt gagcttcttt agtttctcat
      181 cttcatgaaa aaacatggaa ggatggaagc tgtacatgga cgttgactcc atgccaatca
      241 caa
//
LOCUS       DACKAC010000244          237 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.250, whole genome shotgun sequence.
ACCESSION   DACKAC010000244 DACKAC010000000
VERSION     DACKAC010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..237
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.250"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..216)
                     /locus_tag="I0B46_002920"
     CDS             complement(<1..216)
                     /locus_tag="I0B46_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAR2085117.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIF"
BASE COUNT           62 a           44 c           44 g           87 t
ORIGIN      
        1 aaaaatgatt tcggttctcc cacaacgtcg cttattttct ttgtattgtt caaagtattc
       61 aagtgctgtt ccaccacatt tgagaaagtt atagacattg taaattgttt ggcgtccacg
      121 tttaagctgc ttcgcaacga tagcaaccgg agtttcttga tgaaaatatg cttctatcat
      181 tacaagctcg tttggtgtaa gatgggtata agtcatttat gttcactctc cttgtat
//
LOCUS       DACKAC010000245          236 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.251, whole genome shotgun sequence.
ACCESSION   DACKAC010000245 DACKAC010000000
VERSION     DACKAC010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..236
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.251"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            26..>236
                     /locus_tag="I0B46_002921"
     CDS             26..>236
                     /locus_tag="I0B46_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAR2085118.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRL"
BASE COUNT           86 a           48 c           39 g           63 t
ORIGIN      
        1 aaccaaccca aaaggaggaa tcaagatgtc ttacactcac cttatcaaag aaacattaga
       61 tattctagac ttatcagtta cttttaacga aaattgtctc acaaaagaaa aatacaaagg
      121 gcaaatctgt catatttatc gtggaaactt gatttataca gcccaagaat gcatccactg
      181 taaacaccag attgcttcag atattgtgcg ttggggtaca acaaccgtcc ggttgt
//
LOCUS       DACKAC010000246          236 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.252, whole genome shotgun sequence.
ACCESSION   DACKAC010000246 DACKAC010000000
VERSION     DACKAC010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..236
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.252"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..>236
                     /locus_tag="I0B46_002922"
     CDS             <1..>236
                     /locus_tag="I0B46_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAR2085119.1"
                     /translation="NETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYG
                     AGSYANANSPVQIELQHTHDKATFEKNYKAYVELA"
BASE COUNT           74 a           45 c           58 g           59 t
ORIGIN      
        1 aatgaaacag caacaggacg caatgaagcg cagtatatgc aacgttcatg gactagcgct
       61 tatactgctt atattgtggg agacggcgga attgtttatc aagtcggtca acctggttat
      121 gtacagtacg gtgctggttc gtatgctaat gccaacagtc ctgtgcagat tgagttacaa
      181 cacacacatg ataaagcaac gtttgagaaa aactacaagg catacgttga attggc
//
LOCUS       DACKAC010000247          235 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.253, whole genome shotgun sequence.
ACCESSION   DACKAC010000247 DACKAC010000000
VERSION     DACKAC010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.253"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            41..>235
                     /locus_tag="I0B46_002923"
     CDS             41..>235
                     /locus_tag="I0B46_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAR2085120.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYY"
BASE COUNT          103 a           37 c           36 g           59 t
ORIGIN      
        1 atgtttttaa cgacaaaaac atacccagga ggacttttac atgacccaag tacattttac
       61 actgaaaagc gaagagattc aaagcattat tgaatattct gtaaaggatg acgtttctaa
      121 aaatatttta acaacggtat ttaatcaact aatggaaaat caacgaacag aatatattca
      181 agcaaaagaa tatgaacgaa cagaaaaccg acaaagtcaa cgaaatggct attat
//
LOCUS       DACKAC010000248          231 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.254, whole genome shotgun sequence.
ACCESSION   DACKAC010000248 DACKAC010000000
VERSION     DACKAC010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.254"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..>231)
                     /locus_tag="I0B46_002924"
     CDS             complement(<1..>231)
                     /locus_tag="I0B46_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HAR2085121.1"
                     /translation="EDIFAYILEQAVKAGFVTEDNLYLDSTHIKANANKHKFTKEMTH
                     DEAKAYQDELEDEINRQRIEAGKRPFTLDLEK"
BASE COUNT           60 a           48 c           37 g           86 t
ORIGIN      
        1 tccttttcta agtccaaagt aaaagggcgt tttccagcct ctattcgttg acgattgatc
       61 tcatcttcta attcatcctg ataggctttc gcttcatcat gggtcatttc tttcgtaaac
      121 ttatgtttat tggcgttagc tttgatgtgg gtagaatcca gatacaaatt atcctctgtt
      181 acaaaacctg ctttaactgc ttgttctaaa atataggcaa aaatatcctc a
//
LOCUS       DACKAC010000249          227 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.255, whole genome shotgun sequence.
ACCESSION   DACKAC010000249 DACKAC010000000
VERSION     DACKAC010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.255"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..>227)
                     /locus_tag="I0B46_002925"
     rRNA            complement(<1..>227)
                     /locus_tag="I0B46_002925"
                     /product="23S ribosomal RNA"
BASE COUNT           48 a           77 c           45 g           57 t
ORIGIN      
        1 atcgtctgca ttccttagcc tcctgcgtcc ctccattgct caaacaaaaa caacgggtac
       61 aggaatatca acctgttatc catcgcctac gcctgtcggc ctcggcttag gtcccgacta
      121 accctgggcg gacgagcctt ccccaggaaa ccttagtcat tcggtggaca ggattctcac
      181 ctgtctttcg ctactcatac cggcattctc acttctaagc gctccag
//
LOCUS       DACKAC010000250          225 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.256, whole genome shotgun sequence.
ACCESSION   DACKAC010000250 DACKAC010000000
VERSION     DACKAC010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.256"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..>225
                     /locus_tag="I0B46_002926"
     CDS             <1..>225
                     /locus_tag="I0B46_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAR2085122.1"
                     /translation="FFDEEWLIEKEYKGVQAQFIKGKLDDSPSYCEHCRSVRIIRNGS
                     YTTRTQMLKVKEKLTILELKRTRFLCHDCG"
BASE COUNT           72 a           38 c           45 g           70 t
ORIGIN      
        1 tattttttga tgaagaatgg ctgattgaaa aagaatataa aggcgttcaa gcgcagttta
       61 ttaaaggaaa attggatgac tctccttcct attgtgaaca ctgtcgatcg gttcgtatta
      121 tccgcaacgg ttcctatacg acacgcactc agatgttgaa agtcaaagaa aagttaacta
      181 ttttagaatt aaaacgtacc cgttttctct gccatgattg tggaa
//
LOCUS       DACKAC010000251          224 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.257, whole genome shotgun sequence.
ACCESSION   DACKAC010000251 DACKAC010000000
VERSION     DACKAC010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.257"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..>224
                     /locus_tag="I0B46_002927"
     CDS             <1..>224
                     /locus_tag="I0B46_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAR2085123.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAA
                     ESQTPAKPAAEPTVDTESAGSSSEGVFQFK"
BASE COUNT           70 a           47 c           58 g           49 t
ORIGIN      
        1 ccgtcacctg ttacaggaac ggttaaaaat gtgattgttc cagaaggtac cgtagcaaat
       61 gttggggatg tattagtaga aatcgatgca ccaggacacg aagacaatga aggggacagt
      121 ggtgtagccg cagaatccca aactccagct aaaccggctg ctgaacccac tgttgataca
      181 gaatcagctg gatcgtcaag cgaaggagtc ttccaattta agtt
//
LOCUS       DACKAC010000252          221 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.denovo.258, whole genome shotgun sequence.
ACCESSION   DACKAC010000252 DACKAC010000000
VERSION     DACKAC010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.denovo.258"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..>221
                     /locus_tag="I0B46_002928"
     CDS             <1..>221
                     /locus_tag="I0B46_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2085124.1"
                     /translation="IFDPVVAWWNGIWTAISTTASNIWNSISATASSIWNSIKNTITS
                     LVQAAATVIQNIWSTVSSWLGGIWNSISST"
BASE COUNT           69 a           34 c           47 g           71 t
ORIGIN      
        1 atatttgatc cagtagtggc atggtggaat ggtatatgga cagctattag tactacggct
       61 tcaaatattt ggaattcaat tagtgcaaca gcttctagta tttggaacag tatcaagaat
      121 acaatcacta gcttggtaca agcagctgct acagtaattc aaaatatttg gtcaactgta
      181 tctagttggt taggtggaat ttggaattca atcagctcta c
//
LOCUS       DACKAC010000253        18378 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.guided.054, whole genome shotgun sequence.
ACCESSION   DACKAC010000253 DACKAC010000000
VERSION     DACKAC010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.guided.054"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..733
                     /locus_tag="I0B46_002929"
     CDS             <1..733
                     /locus_tag="I0B46_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAR2085125.1"
                     /translation="YGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAI
                     DEIYHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEF
                     IKRDVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMY
                     FQKKLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENW
                     AKRFITNDSTIYEQYEYDLSSVENK"
     gene            complement(883..1824)
                     /locus_tag="I0B46_002930"
     CDS             complement(883..1824)
                     /locus_tag="I0B46_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAR2085126.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            1950..3071
                     /locus_tag="I0B46_002931"
     CDS             1950..3071
                     /locus_tag="I0B46_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAR2085127.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRKNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3329..4831
                     /locus_tag="I0B46_002932"
     CDS             3329..4831
                     /locus_tag="I0B46_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAR2085128.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            4898..5425
                     /locus_tag="I0B46_002933"
     CDS             4898..5425
                     /locus_tag="I0B46_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAR2085129.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            5636..6697
                     /locus_tag="I0B46_002934"
     CDS             5636..6697
                     /locus_tag="I0B46_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAR2085130.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            6700..7596
                     /gene="murQ"
                     /locus_tag="I0B46_002935"
     CDS             6700..7596
                     /gene="murQ"
                     /locus_tag="I0B46_002935"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAR2085131.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            7610..9592
                     /locus_tag="I0B46_002936"
     CDS             7610..9592
                     /locus_tag="I0B46_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAR2085132.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(9657..10103)
                     /gene="nrdI"
                     /locus_tag="I0B46_002937"
     CDS             complement(9657..10103)
                     /gene="nrdI"
                     /locus_tag="I0B46_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAR2085133.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            10314..11738
                     /locus_tag="I0B46_002938"
     CDS             10314..11738
                     /locus_tag="I0B46_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAR2085134.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(11919..12692)
                     /locus_tag="I0B46_002939"
     CDS             complement(11919..12692)
                     /locus_tag="I0B46_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAR2085135.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(12689..13594)
                     /locus_tag="I0B46_002940"
     CDS             complement(12689..13594)
                     /locus_tag="I0B46_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAR2085136.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(13748..14068)
                     /locus_tag="I0B46_002941"
     CDS             complement(13748..14068)
                     /locus_tag="I0B46_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAR2085137.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            14542..15228
                     /locus_tag="I0B46_002942"
     CDS             14542..15228
                     /locus_tag="I0B46_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAR2085138.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            15230..16174
                     /locus_tag="I0B46_002943"
     CDS             15230..16174
                     /locus_tag="I0B46_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAR2085139.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            16373..17017
                     /gene="pcp"
                     /locus_tag="I0B46_002944"
     CDS             16373..17017
                     /gene="pcp"
                     /locus_tag="I0B46_002944"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAR2085140.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVSFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            17096..18373
                     /locus_tag="I0B46_002945"
     CDS             17096..18373
                     /locus_tag="I0B46_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2085141.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
BASE COUNT         5880 a         3253 c         3923 g         5322 t
ORIGIN      
        1 ttatggattt gatatggaga tggctcgacg cgtgcgccca tttaccaatg caaaaattgt
       61 gttatttttt tggaatcact tcaaagcaga acacgaggcg atgctgaagc aggcacaaga
      121 tgaaccggca atcgatgaga tttatcattt tgattttttg gaagcaaaag aacttgggct
      181 gaaacataat agttcatttt attcgaaaca tatgaatttt tcttctagcg aaccaaagac
      241 ggatctattt tttggtgcaa cgaataatgg gagaaaagaa cgagcagaaa gctataagca
      301 ggaatttata aaaagagatg ttacgaccaa ctatttcatt cttccacatc gagggaatga
      361 acaacctgga tatcttactt atgaagagta cctgaaaaaa acaggggaat cgcgcgggat
      421 tctggaactg ttgcgggagg gtcagcaagg agtgactttg cggacatttg aatccatgta
      481 ttttcaaaag aaactagtga cggataatca agcgattgct cattatcatt tttatcgtcc
      541 tgaaaatatt tttttgcttc gagaacgtga tttagatgaa ttaccggaat ttctgcatac
      601 accatttaag ccaatcctat cagagctatt ggaattttat gatgcagaaa attgggcaaa
      661 acgatttatc actaacgatt ccactattta tgagcaatac gaatatgacc tctcctctgt
      721 tgaaaacaaa taaaaaacct acattttgaa gagattatgt actgaaacaa acaagcacaa
      781 ttcttatggc atcatgagag ttgtgttttc tttttgatct tttattgggc ttaaaaaaag
      841 agaaccagct gctgattcag ctgattctca tcagagagcc ttttattctg cgccaaggat
      901 tccttctttt gcatggacta attgggtcaa aaaggcacaa tatggtgtag caatatcata
      961 tttttgtcct tttcgccaaa tggcaccatt gatgtagtcg atttcagtca aacgatggtt
     1021 tttgatcagg tcttggtaca tagatgggta atgcaacccg atgttgtcag gatcataagt
     1081 agaagcaata tgttggtaca cttcttcttg atctagtatg attccttctt ttgctgcgat
     1141 agaagcaaat tcagatacga ttgttcgaac catgctctct gccgcttttt gcgcgcctag
     1201 ttcagcaata ttacaatcca aaatcgtgca taaaccattc aatgttccat ttacacaagc
     1261 ttttcgccaa atggaataac gaacgttatg gctatagatc ggatttaaat ttgcttcttg
     1321 aaagacttct acaacttttt ttgtaaatgc ttcgccttct ggttccaagt tctctagttc
     1381 gatttctcca tcacctaaca atgtgacttt tccgggacca gctagaccag ctgtccacat
     1441 cgtgatccca aataagatat ttttctttgg cacatatttt tccagtacat cttcatgacc
     1501 taatccattt agtaaacaca aaacataagt attgtcagta ataatggatt ggatcgaaca
     1561 aaacatatca tctaattgat tggcttttgt taatgctacg atcaagtcca cgtgttcgtt
     1621 gctttcgatg atttcttctg gtgaatagat tggcagtttt gcgaccactt ctttaccgtt
     1681 aaagtcagca atcaacccat ttgtacgaat ggcttcaatg tgagcaggcc aacgatcgat
     1741 caagaggacc tcatttccgc tttgatgtag catcaatccg attcggctgc ccattgctcc
     1801 ggccccagct attgcgattt tcatgttttt tctctccttt attcaatgag ttccctttta
     1861 gtatatagaa aaatggaaga aaatagcatt gttttgtttg aaaaaaatga aaaaaagtgt
     1921 cttacttaag aagaaagtga gggatacaga tggatatttt ggcggcaatt gattcattta
     1981 aaggaagtgc ttcttcattt gaactaaatc aggcagttct aaatggctgg gaagatcgta
     2041 aaggaagaaa aatcaatgtg ccgattgcag atggcggaga aaatactgca gaagcaatct
     2101 attacgcgct caggggaaca tggcgtacca ttgatgggat ggatctctta ttccgtcagc
     2161 gaaaaatcag ctatttattg acaacttggc aaggaaaaaa caccgcagtc atcgaatctg
     2221 cacaagcatt ggggttagat ctacttgata aaccaacgga tcaaactatc cgacttgctt
     2281 ccagttatgc cttaggagaa cttattaaag atgcactgct tcaagaagta gaacaaatta
     2341 ttgtcacttt aggaggaagc gggtgtagcg atggtggtct aggattactc caatccttgg
     2401 gtgcttcttt agaagggtac acagaaggaa atcctttatt atctactaaa aaacttcatt
     2461 ttgatcgaaa aaacgttctt gtttctaaag taaaattgat tatcgctgca gatgttacca
     2521 atccatatac aggtcaacaa ggagctgctt ggcaatttgg aagacaaaaa ggaggaatag
     2581 atacgacgct agctttctta gataaacaag caggaaagat tgctcagcaa ataaaagatt
     2641 tctgggaggt ggatttagat gatattccag gaagtggagc agcaggcggt attggtggag
     2701 cccttttttt gttgggagcg aagatggttt ctggtttcga tttagtcagc caaatcactg
     2761 gattggaaaa aaagatcgaa caagcagatg tcatcattac aggagaaggg cggatcgatc
     2821 agcaaacgat ccacggaaaa gttccatacg gtgtagcagt gcttgcaaaa aaatatcgaa
     2881 aaccaataat cgctatttgc ggaagtagag accaagagct agaaggactt tctgattttc
     2941 tcccaattgt cttaagtatt catttgggtc caataggttt agaagaagca atggaacaca
     3001 ggcaaacatt agaaaaaaca aaaatcttag gacagacact tagcagattt ttcaccatcc
     3061 tcccgaagta gttgacaaaa tcattttctt tatgctatcg taatttcaaa tataggacaa
     3121 agtgagggat ccgtgcattg aaaaattaag tcgattgatg aaagctgcat tcagactgta
     3181 ccttgtggag acaaggaagc agtgtgcaga ttttcgggat tggcttggtt ttttcgatgt
     3241 atgggtcttt ttgtgtgtat ccaacccttg catcctcttt agccagttct gaaaatagga
     3301 ctggtctttt tttacgaggt gatggaaaat gtctaaaatc gaaataaaaa atctgacatt
     3361 cggctacgac agccaaggca cattattatt tgaacaagca aatctaaatt ttgacacaca
     3421 atggaaacta ggacttatcg gacgaaacgg tcgaggaaag acaactttac tgaatattct
     3481 acaaaacaaa ctaccttatc aagggcaagt aatccatcag caagaatttg cctatttccc
     3541 gcaacagaca aaagataaag aacgtttaac ctattacgtg ttaaatgata ttacggattt
     3601 tgagatatgg gaaatcgaaa gagagctcca attgatgcaa acagatcctg aaatcttatg
     3661 gagagaattc agcacactat cggggggaga gaagacaaaa gtcctactgg cacttttatt
     3721 tgtggatgac actcatttcc cgttaatcga tgaaccaacg aatcatttgg atatctctgg
     3781 tagaaaacaa gtagcggctt atttgaaaaa gaaaaaacaa ggcttcatcg tggtcagcca
     3841 tgaccgggga tttatcgatg aagtagtgga ccatgtttta gcaatcgaaa aaagtcaact
     3901 ggaactttat caagggaatt tctctatcta tgaagaacag aaaaaacttc gtgatgaatt
     3961 tgaaatggct caaaatgaaa aattgaaaaa agaagtcagt aggctaaaga aaacagcagc
     4021 tgaaaaagcc gaatggtctc gttcccgaga aggagataaa acaaagaaac aagtcggatt
     4081 catcgatact gaatctagac gagtgaataa aggagcagtg ggtgctgatg ctgcacggac
     4141 gatgaaacga tccaaagcaa tcgtgaatcg gatggagacc cagatcagcg agaaagaaaa
     4201 actattaaaa gatatcgaat atatcgattc tttgacgatg aatagccaag cgtctcacca
     4261 taagcgactt ttaagcgtag aagatcttca attagggtat gaaaatctgt tattcgagcc
     4321 aattcatttt acaatcgagc ctcatcagcg ggtggcgatt tcaggtccta acggtgcagg
     4381 aaagtcatcc attatccatt atcttctggg ggcattcaac ggcaaggtta taggagaaaa
     4441 ataccagcca aaacatctga gcattagtta tgcaagccaa aattatgaag acaatcgagg
     4501 aacgttggcg gaatttgcag agaaaaacca agtagactac caagcatttt tgaacaacct
     4561 ccgaaagctt gggatggaaa gagatgtttt tcataacaag atcgagcaga tgagtatggg
     4621 ccaacggaaa aaagtggaat tggctaaatc tttatcacag ccagctgaac tatatacatg
     4681 ggatgaacca ttgaattatt tggatgtctt caatcaagaa caattagaac aactgatctt
     4741 gaacgtgaaa cctgccatgt tactagtgga acatgatcaa accttcctgg ataaagtatc
     4801 tactgagatt atttctcttg agagaatcta acccttgtaa tctcaagaac aacaaggtat
     4861 ggttaagggg aactttgcca acgaaggaga gaatcacatg ctttatgtaa cgagacacgg
     4921 agaaacaact tggaacgcac aaggattagt ttgcggacgg gcagatgtgc cgctgactga
     4981 aaaaggacag atgcaggcac agaaactagc agaaaaggta gtagatttgc ctgttccaat
     5041 cacgaaaatc atccattccc ctcttcaacg agcaagagat acagcacaag cagttgctga
     5101 tcgtctcagc ttgccgctga ccgttgatga acgattggta gaaatggatt ttggggatta
     5161 tgatggactg ccaagcaaag atgagaactt tcaaaaagca cgtttagcgt tcgctgtacg
     5221 attcccaaat ggggagtcag tcttagatgt gtatgcacgg atcgttcctt tattgaaaga
     5281 atgcatcgaa gacgaagaaa atgtctatct gttagtttgc cataatgcct tgataagagt
     5341 gatcaatgct tattttcatc cgatgcctaa tgaggggttc tttacattca tggtagataa
     5401 tacagaattg gtttcttatg agtaattatg tttttgtgtt atttaaataa aatatttcaa
     5461 ggcactgctt ttgcttagca gtgccttttt taggttatga caattctttt gataatataa
     5521 actcaacctt tagaaatgaa atttaattat tttaataaaa gtattgaaat tttgtttctt
     5581 tagatatata ctctagtcat agaaatgaag acggatacag aaaaagaggg ataatatgtt
     5641 tggcttttca atatttatga acgattcttt gtcagaagag aagaaacagt atatccgcca
     5701 aatggcatcc aatggctttg tcggtatttt cacttccatg catataccag aagatgatgt
     5761 ttcggcatat caaaagcgat tgatcgaact aggaagcgaa gcgaaaaaga ataaactaga
     5821 attgatggtc gatatttcag gagaggcatt ggaacgagcg ggtttttcat tagaagatct
     5881 aacgcctttg aaagcaattg gtgtaacagg tttaagaatg gattatcata tttccaatca
     5941 gcagattgcc gactggtcac atcaattgaa aatcagtttg aatgctagca cgatcacgcc
     6001 taaagatatc gatgaattga aagaagcaga agctgatttc tcacagattg aagcttggca
     6061 taattattat ccacgtcccg aaacagggct ggacaaagaa tggtatcaga aaaaaaatca
     6121 atggctcaaa attcaaggtc tccttgtcca aggttttgta ccaggagata cagagttgag
     6181 aggcccacta tatcaaggat tgccaacttt ggaagaacat cgagggatcc atcctcttgc
     6241 aggcgctcta gatctattag caagtaacac ggatattgtt tacatcggag atgcaggtct
     6301 gtcagaaaac gttcaggagc aatttgcttc tttccaaaaa gaacagacag tcttgctcca
     6361 tacggaacct gtagatgaag aattttacga atatatatta gggaagcata cgaatcggca
     6421 agacgatgca agagatgtca tacgaagcgt agatgcacgt tttcgagaga ttccgcctat
     6481 cccagcaaga aatacagcta cgcgtatgaa agggtctatt actttagata acgaaaagta
     6541 tttgcgctat atgggtgaga tccaattaac caaatatgat ttgccagcgg atgaaaaagt
     6601 gaatgtagta gctaaagtta tcaaagaaga gctaccattg atcaaccaaa tcaaagctgg
     6661 tatgaactat caatttataa gaaaagaagg aagataaaaa tgagcaaaat caatttagag
     6721 aacctaacaa cggaacgcag aaaccaagaa acttttggat tagatgagat gagtatttct
     6781 caagccgttc aactaatgaa caaagaagat cagaaagtgg ccgctgcggt tgaaaaagaa
     6841 ctgacaagta tcgaaccagt gattgctaac acgattgagt cctttaaaaa aggtggacgt
     6901 ctgatttata tgggagccgg aacaagtgga agactaggtg tgttagatgc tgctgaatgc
     6961 gtaccgacat tcggtgtaga accggaaatg gtcataggtc taattgctgg tggagaagaa
     7021 gcaatgactg tagctgtcga aggggccgaa gattcttatg agctagggca agaagatcta
     7081 atcaagctga atttgacaaa aaatgatata gttattggta tcgcagctag cggtcgtaca
     7141 ccatatgtga tcggtggatt agattacgca aaatcgatcg gtgctgtcac aggaacgatt
     7201 tcttgcaata aacaagccga aatcagtaaa cacgcggatt tcccaatcga agtcgatgca
     7261 ggtccagaat ttttgacagg ctctactcga ttgaagtcag gaacagcaca aaaacttatc
     7321 ttgaatatga tttctactat cagtatgatt ggtataggta aagtattcaa taacttgatg
     7381 gtcgatgtca aaccaacaaa tgaaaaacta atcgaacgct caaaacgaat catcatgcaa
     7441 gcgacagaag ctgattacga aacagctgaa aagtactttg aagaagcaga tcaaaatgtt
     7501 aagctggcta tcgtcatgat tttgacaaat agtgataaag aggaagctca agcaaaatta
     7561 gctgaagcaa acggatttgt aaaagggacc ttatgagggg gaaaagacca tggcaaaaaa
     7621 cgaactgact ttggaagaac tggcaaaaca aatttataaa ggggcaggcg gcatgggcaa
     7681 tgtagaaagc attgtccact gcatgactcg tgtgcgtatg aagatcatca atgatgatct
     7741 tgtaaatgtc tctgaattaa aagcgattcc aggagtgctt ggtgtagtag aagatgaaca
     7801 gcttcaagtc atcatcggac ctgggaaagt gaacaaagta gcacagcaaa tggttgattt
     7861 ggcaggcgtg aaattaggag aaaaactttc ttctgtaagt acagctacag caaattcatc
     7921 agcatctgga aaagaccgtg taaacgaacg ggcacaagaa atgaaagcgg cacaaaaatc
     7981 gaaacaaaaa ccatctaaaa tcaaatcggt attaaaagac atttctaata tctttgtgcc
     8041 aatgattcct gcatttgtcg gtactgggat cgtggcaggg attgccgcag ttttagcaaa
     8101 cttagtcaca gccggaacac ttgatgcagc aacatggcaa caatatatcg atgtgatgaa
     8161 catcttgaaa aacgggatgt ttagctacct agttatctat acaggtatca atgctgcaca
     8221 agtttttggt gcaacaccaa ctcttggtgg agttatcggg gccgttgtga tgctgacagg
     8281 gatgaatccg gaagcaccac tgaaaaactt atttactggt gaagctcttg cagctggaca
     8341 aggtgggatc ctaggtgtca tcttcgctgt ttggctattg tcaatcgttg agaaaaaact
     8401 gcataaagta gtacctgatg cggtagatat catcgttaca ccaactcttt cattgattgc
     8461 catcggtctg attgaaattt tcttcatcat gcctttagca ggtttcattt ctgatggcat
     8521 ggtcggtggg atcaactggg tacttggcgt tggtggagca tttgccggct tcgttctagg
     8581 cacattattc ttgccgatgg ttatgttcgg actgcatcaa attttgactc caatccatgt
     8641 gcaaatgatt gatgaaactg gtcgtacgct attgcttcca attcttgcga tggctggtgc
     8701 aggacaagtg ggtgcagcat tggcattatg gattcgctgc aaaaaagata aagaattaac
     8761 agaaatgatc aaaggagctt taccagtcgg gatcctagga atcggcgaac cattgattta
     8821 cggagtaaca ttacctctag gacgcccatt tattactgct tgtatcggtg gaggtatcgg
     8881 cggggcagtg atcggaatga tcggaaatgt cggggctatc gctattggac cttcaggagc
     8941 cgctttgatc ccattgatct ctgacggtaa atggttaggc tacatccttg gattattggc
     9001 agcttatgct ggtggcttcg ttgctacatt cttcttcggt attcctaaag accgtttaga
     9061 aaaagaagaa ttagcgactg aaacagccac agtaaatgaa gtgcctgaaa aagtagtacc
     9121 tcaaacttca gctgaaagtg tgactatcct ctcatcagtt gctgacggaa cagttgtttc
     9181 tttagataaa gcaagtgatc cagtttttgc acaaaagatg atgggtgaag gctactttgt
     9241 agaaccagaa aacggacata tctattctcc agttactggg gtagtcagct ctgtcttccc
     9301 aacgaaacac gctattggga tcacaacgcc aaatggttta gaaatcttgc ttcatatggg
     9361 aatcaacaca gttgacttag gcggtacgcc atttgatgtg aaagtaacag aagggcagca
     9421 agtgacacct gatacgcttg tagcagaagc tgacttagaa gcgatcaaag cagcaggcaa
     9481 agaaacatcc atgatcgtct tggtaaccaa tatggatcgt gtaaaaaact ttgtcttgga
     9541 aaaaacaggt aaagcaaaag caaaagcacc tgtgatggat gtcaaagctt aatatgatag
     9601 aaacaataga aacgaaagaa gaaactgaaa tctatttttc agcttcttct tttttattat
     9661 ttattgtttt ctgctaaaat ctgataaatt cgtgtcacat cagatggtgt tccccgtaat
     9721 tcataatctg ccaagaaagg aaagccaaat gtttgtgcat attgtttggc tgttagacaa
     9781 tactgattat tgaagttttt atttccgcta ccgactacac caagacaaag tttatgattg
     9841 tcttcatgtt ccagatattc acgcatcgtt tcggtcaaga tttctgtatc accgttatct
     9901 aaaccatttc cgccgtctaa ataagtagga acgaaagtaa aaaatggttc tgtttctttc
     9961 gtaaaatcac tattttcatg gatttctttt aaagtaactt caggattgac aggatcttct
    10021 gcatgcattt tttcagcata ttctgctaga tgtttggcaa aggcacgcgt atttcctgaa
    10081 atcgatatat ataaaatgtt catggatcat cctccaattc tttcctttta gtatagcatc
    10141 ttttttaagt tttttggtgg tgtaatttgg aaagggacta ggcgctttgc ttgtataatg
    10201 agaaaaaagc cggtagaatt atatttctat taaagttttt attaattaag taaaaataaa
    10261 aaaggtttgt tatacttttt gataaaagcg gttttatttt ggaggatgct tacatggata
    10321 cagagcaaca gaaaaaagga attggtttaa ttccacttgc tgccttagta attggttcag
    10381 cgattggagg tggcgtattt ggtattatga cggacatctc cggatcggct ggcggcccag
    10441 ctattttcag ttgggtactc gtaggaacag caatgttgat gctctccttg tcgatcaata
    10501 atatcaataa aaaacgtcca gatttaaaag gcgggatctt tagttacgca gaagaaggtt
    10561 ttggccgttt cgctggtttt atcagcggtt ggggttactg gctcacatgc tggttaggca
    10621 acgtagcttt tgcaacattg ttgatgagcg cattaggata tttcttccca gtatttgaag
    10681 gtgggcaaaa tctggcatcg atcgtgatga gtacagtctt tttatgggga tatgcatggc
    10741 tagtgaatcg gggagtcgaa aatgctagtt tcgtcaatgc tatcgtcaca ttctgtaaat
    10801 tagtgccatt atttttattt attgtagtag ctatcagcac gttttcgatc caacaattca
    10861 ttgataactt ttctagcgac attatattga atgcgaatgg acagccgatc ccgttttcca
    10921 gtcaagtatt gagttgcatc atggtgatgc tttgggtatt tgttggtatt gaaggagcct
    10981 ctgtcgtagg ttcacgagca aaaaaacaat cagatgttgg gaaagcaacg attctaggga
    11041 tattcggctt gattattatt tacgtcttgg tttccatgct tccatatggg acaatgacga
    11101 tgaatcagct gcaaacgatc aaggcgcagc ctgctatggg ctatgtgttt gaaatgatgg
    11161 ttggtaaatg gggagcagtc gtgatcaacg gcggtttgat catttcttta gttggtgttt
    11221 ggctctcatg gacgatctta ccgattgaaa caatgggaaa catggctgaa gacggacttt
    11281 tgcctaaagc atggggaaaa ctcaacaaaa atggggcacc aacttatgct atcgtattga
    11341 cgacgttatg tacgaatgtc tttttacttt cattattatt cactgacagt gcttataact
    11401 ttgcttacac acttggtaca gcagctattt tcttcacgtg gcttttctta ggattgtacc
    11461 aaatgaaact ttcttatcag cggaaagaat gggtccaatt tttagtaggg cttttagcaa
    11521 gtgcctttca agtatgggct atgctagtcg tagcctttaa tgaagtaatg cttgttcttg
    11581 ttcttttctt gccaggcatt tatttttacc tcaaagcacg taaggagcaa aacgagcctg
    11641 tacaacatat ccacttagat aagattttac ttataggtgt gacgatggtc ggagtgattg
    11701 caatggtttt atttgccact gggatgcttc acgtatagaa gtataaaaga agtataaaga
    11761 gaggttgtga gcctgtcgtt tattcaaaaa catactagga aaggagctgt gacacaaatg
    11821 ttgtcacagc tcctttttca gaataaacgg tgtttaagag acagccaatc ggcaataagg
    11881 ctgaacgtct ctttcacagc ctcttttttg atgagtcatc atacattttt tcgcttaaat
    11941 acgagatatc ctaatgctaa gaacaaaacg atgtaagcta aattaccgaa tagcatttga
    12001 ttagtagaaa gatgagtcaa ttgcttcatg atctcttcgt ttccaatttg gttttgcaga
    12061 ttcaacatac tgatcggatt ccatttgatc cactcccatt tttggatgag tgcaataagg
    12121 ataccagata tgattgacga agcaaaataa aagacgattc ctgctgagat ggatgcgccg
    12181 gaactattga taaagcaagc aatcatcaac actaaactta aaatcagcca taaaccgata
    12241 taactaccca aagtataagt gaacaagctt tggatcagtg tttgaccagt agaaaccttc
    12301 tccgtgaagg aaatcgaagg gaaaagaatc agtttcatca acacagtgac aataatagct
    12361 aaaaggtata aataaacaga ataaataact aatgtgatcc atttgcttac taatatttct
    12421 cctcgagaat actttcgata aaggagattt ttcaatgtac catattgtgc ttccatcgaa
    12481 atgatcgtgc ttgcagctgc aatcatgata aagacaatcc aagatgtggc actaaaaagc
    12541 tgagcagtca tttcaactgg atcaatgatc gtcgtgtatt ttttggctag aagtgctgtt
    12601 cctatgagca aaacaactaa tagaacagat aaaacagcag ttgacttttt cttgatcaat
    12661 ttaaataatt cttgttttac aagcaatgtc atttttcttc ttcccccttt actgaatcca
    12721 gaatttccaa tagagaagat tccaaatcat tcgtcagatg ctggacgtct gtcacatgga
    12781 tattttgtgc agacaatagc tggatcacgg ttcctaataa ttcttcgttt tcttttaaaa
    12841 gcttgatttt atcaggatct tccaaagtat atccagcttc tgtcaagatc gttcgagcag
    12901 ctagatcatt tgttgtagag ataacgatga aatcttgcgc tgtttggttc aattcagcca
    12961 ttgtacattg tttgatgatc tttccttgat tgatcaggat cagctcatcg accatttttt
    13021 caagctcgct gagtaagtga ctggagatca agaaagaggt gccctgagta gccaagtcgg
    13081 taatgatttt tcgcacatct cttgttgctt gtggatccaa accgttcatc ggctcatcta
    13141 aaataacgat agacggatgg ttcagtaagg caagagcaat tcccaatttt tgtttcattc
    13201 ccaacgagta ggatttcgct tttttgtgaa tgtacttttc catcttgagc tgattcacga
    13261 tagtgtcgat agcagataca tcgttggttt cagagaataa cttcagatga tcatagcctg
    13321 ttaagaatgg atagataccc gggtattcga tcaatgctcc tacttgttcc aaccctttat
    13381 gactagaggg agaaatctca ttttcaccaa tacagattgt tccatctgag taatggatca
    13441 gtcctaaaat agctttcatg atcgttgttt ttccagcgcc attaggacct actaatccaa
    13501 cgattttgcc aggggataag ctaaaactga catcgctgag ggcttgtttg tgaccaaagt
    13561 tcttggataa ttcttggacc gaaagacttt tcaaaaatat tcactccttt tctatagata
    13621 gtacaagcat acacctgatg agtggagaag tcgctaggtc attggactta tttcttattt
    13681 tttcataaaa aaattgaata ttaaataaaa aggcttattt tttctgatca gtacttattc
    13741 caagtgtcta gtggccgcta gtaatcttga tgccaatgat actgatgagt agcatcccca
    13801 caaagaacca agtggaaaga ttcagcttat catgaaacaa gaccacacca attatgaccg
    13861 aaccaactgc gccaatccct gtccagatag gataagagag actcatcggc aaacttttga
    13921 ttgcctttga caaaaagtaa aaactagcaa tcatgccaat cactgtgtaa aaagaataat
    13981 tgattttaga aaaaccatca ctccatttca tcatcgttga ccagaatact tccaaaattc
    14041 ctgctatgat taattccatc catgccatac ttacattcct ccttaactat aaaaaaatac
    14101 acaaaaagag gaaaatcatc ccttcaaaga cctaatggaa tgatcatcct ctttatccta
    14161 cccggtggca gattgtatat ctttttctgt agtatagcac acctatccag atatcggcaa
    14221 tctcatactt tttagtaaag tgtaccaagc actgacggat atatcgttag cggaaaagaa
    14281 gatgaatgct ttgcaatttc atagaaagaa gttgttattt attaatcatt taaaaaaaac
    14341 agtaactaga ccttatttct atatattatc gggcaataaa ttaaaaaaag ctttacctta
    14401 caataagtaa gataattttt gtgataaaaa aataacgaaa aatatttaaa aatagtgtct
    14461 atttcgtgag aaaacaacta tattttttat gtgttttgct ttataattaa aaaaatattg
    14521 tcattttatg gaggaaaata tatggaaaat tatcttagcc ttttaggcgt cttgattgtt
    14581 attctcgggt ttgcatttaa attagactcg atattgatcg taatggttgc tttagttgtt
    14641 acagctttga ctggcggtct tggcattcaa ggaatgttag aacttttagg tacaagtttt
    14701 gtaaacaatc gcgggatggc gatttttatc atcattatgt tggcaacagg aacgcttgaa
    14761 agaaatggtc tgaaagaatc agcggcttcc ttgatcaagc gattcaagaa agtatctgct
    14821 ggtatgattg tagatattta tgggatcttt cgaatgatct ttgcggcttt taacgtgagt
    14881 tttggtggag ttgctggatt tgttcgtcca atcattttac cgatggcatt ggggaccatc
    14941 gaatcaaaag ggttagaagt ccatcctaag catgaagaag aactaaaagg tatggcttca
    15001 gctatggaaa atgtctgctg gttctttggc caagtactat tcatcggagg agcaggggcg
    15061 ttgctagtcc aatcaacact gaaagatcta ggatatgacg taacattggg acagcttgct
    15121 ttagtggaag ttccagtggc tatcacggct ttagttgtgg caagtgtcta cttctatctt
    15181 aaagaaaaga aattagcgaa gaaatattat ccaggaaagg ggcaataaga tggacttttt
    15241 tacaaatcct gatattttat taggcgataa attactggag atcctttata tcgtgatggg
    15301 actcgtattg atttacactg gagtaagaaa tttaacagat aaaaccaatc ctcatcgtta
    15361 tgggtcagcc tttttctgga cggtcttagg ggtagttatt gcaggaggaa gatggcttcc
    15421 ggcaatcgtg aacggtggct tgatctttgc gatgaccata ccagcaattg ctaagaaagt
    15481 cagcaaagga gaaagccggc ttccatcaaa agaatatatg gaaaaaatgt cagacaaact
    15541 aggaatgaaa atttttattc cagctttgag tatcggtgtc tttgcgatct tgtttgctct
    15601 atttaccaat ttaggtgctt tagtcggcgt gggcgtcggt gtttttgcag ccatgctaat
    15661 cttgatgttc ttttctcgag ataatcgacc aagtacattt ttagatgatg cagcagatat
    15721 gctgggcaca gtagggccgt taagcatgtt gccaatgctt ttagcttctc taggtgcggt
    15781 atttacaagc gctggagttg ggacagtgat ttccaatgca gtaggtacga tcgttccaga
    15841 aggaaatgtg aacatcggca tcatcattta cgctcttggc atggtgatat ttacggtaat
    15901 catggggaat gcatttgcgg ccatcaccgt gatgacggtt gggatcggtg caccattcgt
    15961 atttagtcat ggagcgaatc cagcgttaat aggaatggtt gcattgactt gcggtttttg
    16021 tgggaccctg ctgacaccga tggctgctaa cttcaatatc gtgcctgtag cgatgctgga
    16081 gatgaaagac aaatacggag tgatcaaaaa ccagttgttt attgcgttat ttatgctagt
    16141 tttccaaatt gcttatatga ttctatttaa ataatagaag agaggggttg tgacagaata
    16201 attgtcgcaa cctcttctaa cattgtagtg gaacactatt tttttataag aatatattaa
    16261 gtttgatctg ttatgttatt taaactattg tttcctttat ttacgctgtt caaggaaaat
    16321 ggtatgataa aagataacca tatgttaaat caatcggacg gaggaacaac agatgaaagt
    16381 actagtaaca ggttttgatc catttggtgg ggacaaagtg aatccagcct atgaagcagt
    16441 aaaaaaaatg ccagatgaaa tcagcggtgc tgaaatcatc aaagtggaag taccgaccgt
    16501 attcgaaaaa agcagtcaag tagtcaaaga agccatccaa cagcaccaac cagatgtcgt
    16561 gatctgtgtc ggacaagcag gtgggcggtc agcagtctcg ttcgaacgtg tcgcaatcaa
    16621 tctagcagaa gcaagaatcc ctgataatga aggaaatcag ccattcgata cggctttaga
    16681 agaaaacggt ccagccgctt actttacctc attgccaatc aaagcaatga caaaaaatgt
    16741 acaagatcat ggtctaccag cgtacatctc ttatacagca ggaacatttg tctgcaatga
    16801 tatcatgtat cgtttgcttc atttgatcga aacacaattc ccggcaattc gaggtgggtt
    16861 catccacgtg tcattctcac ccgatcaagt gatcgatcgt ccagtgggta cgccttctat
    16921 gtctttagaa gacattgctt cttctttgac ttatgcagtt gaagcagcag tagaaaataa
    16981 agaagatatt tccggaaatg ccggaacaac tcactaattt agcaagaaac actggctggt
    17041 tgctaacgtt atgttagtag ccagcttttt gtaattagaa aggaaagaaa cagccgtgga
    17101 agaatggcaa tccgtctttg aagaatggtt tccaaaagaa atcagcaaaa gctatcccat
    17161 caaaatttct aaacaataca caagcagtca acgttgggag atctatgcaa aattaacgaa
    17221 aaaacagcgg gaactagtcg ataagcaccg ccgatatcta atcagctctc gttttatgga
    17281 agaacattat ttagctgcaa cagattgggt gttttctgac ttcaagatca atccgttttt
    17341 tcgaacaaaa cgaagccagc aaaaattgta ttgtgagtgt ggtagagaat taaaagtgca
    17401 atacatcgtt aaatcgccaa aaaccggaaa gatcctaaaa ctaggcatca accactttgc
    17461 cgatcacctg catgtctctc cgacagtggc agcttcgatc catcagggaa tgacaaaagt
    17521 tgatctggcc ttagacgaat tattatggct taagcagaag aatatcgatt ttcctgaagg
    17581 actatggcaa aaatactgtt ttgtcttgta ccaaaatcgt cggatgaaac agccatattt
    17641 accggatata aaattagctc agcggctcgc agaatttcgc caagtagaaa tgccgattta
    17701 catagcggat tatcaagcat tggaaaatga aatcaagaaa ataagtgaac acatcaatgg
    17761 gcagtcaaaa aagcgacaga taaaaaaaga actatttgac gactttgcag aagaattagt
    17821 aaaggatgta gaagaattct tgatcaacta tcgcgcattt ttacgtaaag attggcaatc
    17881 catcgtatat gaagaagtgc cagtacatcc taatgcttat tttgaaacat ttatttccgt
    17941 gttgagaaag acaaaacgtc aacgaacacc agaagtcact gctcagatgg aatattttgc
    18001 gaaaaaccaa cgattcatcc aaccaaaaat ctatctattt atttggaagc aatattgtcg
    18061 ctatggtttt acagaagggt tcttcgacag cattcctcgg atagtcagaa atggtttttt
    18121 aaaagtattg cgtaaagaac gagaagctat acaatctgct gataagaaag accgtacagt
    18181 ctcaaaagaa aaatggcaac tagtagtgaa agatatccaa tctggaaatg tgcaagagac
    18241 aatcgacaaa tggaaaggaa aacattatcg ttttactgaa gctcagaaac aagcgttgga
    18301 atattatcag aagttagaag aatcactgcg tttcaatgat gaggcaagaa agtatctgaa
    18361 ggaattacta taaaaaat
//
LOCUS       DACKAC010000254         8948 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.guided.093, whole genome shotgun sequence.
ACCESSION   DACKAC010000254 DACKAC010000000
VERSION     DACKAC010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.guided.093"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..937
                     /locus_tag="I0B46_002946"
     CDS             <1..937
                     /locus_tag="I0B46_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAR2085142.1"
                     /translation="GRWKETLPEISHEINIVNLTSDEKNKGLLDPYVIMKNTKDSESL
                     AIDILTFLTGISSRDGERFPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADH
                     IESFTDYDFAHLLFSDGYVEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVA
                     MLIVISTFALDFIHTDRSIFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFV
                     TQNTDDLLDEKLKNNLGLKFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQC
                     LISDLYGRVGVIQFHPVFEELLHAFDTRPPVRKEV"
     gene            940..3123
                     /locus_tag="I0B46_002947"
     CDS             940..3123
                     /locus_tag="I0B46_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAR2085143.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            3120..4121
                     /locus_tag="I0B46_002948"
     CDS             3120..4121
                     /locus_tag="I0B46_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAR2085144.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            4136..5050
                     /locus_tag="I0B46_002949"
     CDS             4136..5050
                     /locus_tag="I0B46_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAR2085145.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            5295..5411
                     /locus_tag="I0B46_002950"
     CDS             5295..5411
                     /locus_tag="I0B46_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAR2085146.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            5427..7343
                     /gene="tet(M)"
                     /locus_tag="I0B46_002951"
     CDS             5427..7343
                     /gene="tet(M)"
                     /locus_tag="I0B46_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAR2085147.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYSP
                     VSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIVD
                     TQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRPN
                     SRIDKVRYMFNKIT"
     gene            7441..8814
                     /gene="tet(L)"
                     /locus_tag="I0B46_002952"
     CDS             7441..8814
                     /gene="tet(L)"
                     /locus_tag="I0B46_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAR2085148.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
BASE COUNT         2830 a         1584 c         1867 g         2667 t
ORIGIN      
        1 tggcagatgg aaagaaacct tgccagagat ttcccatgaa atcaatatcg tcaatctgac
       61 ttctgatgag aaaaacaaag gcttacttga cccttatgta attatgaaaa ataccaaaga
      121 ttctgaatca ctggctattg atattttgac attccttacg gggatttcct ctcgtgatgg
      181 ggaacgcttc ccaatcctta gaaaagccat tcgtgcagta accaatagtg aagtgcgagg
      241 gttgatgaaa gtgattgaag aattacgggt tgagaatacg ccactaagta ccagcatagc
      301 cgaccatatc gaaagtttta cagactatga ctttgcccat ctgcttttta gtgatggtta
      361 tgtagagcag tctatcagcc ttgaaaaaca actgaacatt atacaggttg ccgacttggt
      421 acttcctgac aaggaaactt cctttgagga atataccaca atggagttac tatcggtagc
      481 aatgctgatt gtcattagta cctttgcgtt ggactttatc catacagacc gaagtatttt
      541 caagattgta gacttagacg aagcatggag cttcttacag gtggcacaag ggaaaacact
      601 atctatgaag ctggttcgtg ctggtcgtgc tatgaacgct ggggtatatt tcgtgaccca
      661 aaatacagat gacctcttag atgaaaaact gaaaaataac ctcggcttaa aatttgcctt
      721 tcgttccact gaccttaacg agattaaaaa aacccttgcc ttttttggtg tagacccaga
      781 ggacgaaaac aatcagaagc gattgcgtga tttggaaaac gggcaatgcc ttatcagtga
      841 tttatatggt cgtgtcggtg tgatacagtt ccaccctgta tttgaagaac tgctccatgc
      901 ctttgatacc agaccacctg tgcgaaaaga ggtgtaaatg tgaaaccatc aatattaaac
      961 agaataaaat caaactggac gctgaaacgt ctaggtaaag tggcaatgac agtggctttc
     1021 acacttgtga ttgccatttt tcttttagcc atgctgggaa cggtggttca agctgcgggc
     1081 ttggtagatg atacggtcaa tgtggcaaat gaatacagcc gatacccact tgaaaactat
     1141 caactggatt tttatgtgga taatagctgg ggctggctac cgtggaactg gtcggacgga
     1201 attggaaaac aagtcatgta tggactatat gccattacca attttatttg gacaatcagt
     1261 ctttatgttt ccaatgcgac gggttactta gtacaggaag cctattcctt agacttcatt
     1321 tccgctacag cagattccat tggcaagaat atgcagaccc ttgcaggggt tacgcctagt
     1381 ggactatcaa cagagggttt ctatgttgga ttccttttac tcttgatttt ggttcttggg
     1441 gtttatgttg cctatacagg actgataaag agagaaacca caaaggcaat tcatgccctc
     1501 atgaattttg tgctggtttt tatcctatcg gcttccttta ttgcctacgc tcccgactac
     1561 attaaaaaaa tcaatgactt ttcatcagac atcagcaacg ctagtttgtc actgggtaca
     1621 aaaattgtca tgccccattc agatagtcaa ggcaaggaca gtgtggactt gatacgtgac
     1681 agcctatttt ccatacaggt tcagcaaccg tggctactgc ttcaatataa cagttcagac
     1741 attgaaagta ttggtattga ccgtgtggaa agcctgctct caaccagtcc agattccaac
     1801 aatggggaag acagagaaaa aattgttgcg gaagaaattg aagatagaag caataccaat
     1861 atgacgatta caaagacgat taaccgttta ggtacagtct tcttcctatt tgtcttcaat
     1921 attgggattt ccatatttgt attcctatta acaggaatca tgattttctc gcaggtactt
     1981 tttatcatct atgctatgtt tctgcctgtg agctttattt taagcatgat tccatcattt
     2041 gatggtatgt caaaacgagc cataacaaag ctctttaata ccattttgac acgagctgga
     2101 atcacattga ttattacgac agcatttagt atttcaacca tgctctatac cttatcggct
     2161 ggttatccgt tctttttgat tgcttttcta cagattgtga cctttgcagg aatctacttt
     2221 aagctgggcg atttaatgag tatgttttct ctacagagta acgattctca aagtgtggga
     2281 agtcgtatga tgagaaaacc tcgtatgctt atgcacgctc acatgcaccg tctacagcgg
     2341 aaacttggac gctccgtgac tgctttaggg gctgggtctg ccattgccag tgctacgggt
     2401 aaaaaaggac agtcgggttc gggaagttct gcgagtacac aagcagatca ctctcgacca
     2461 aacgggcagg aaaaatcaac actaggaaag cgtatcggtc aaaccatcgg tacagtagct
     2521 gataccaaag acagaatggt agatactgct ggtaatttga aagaacaggt taaggatttg
     2581 ccgaccaatg caagatatgc agtatatcaa ggaaaatcta aagtgacgga taatgtccat
     2641 gatttaacca gaagtatttc tcaaaccaaa gcggataaag ccagtggacg caagaaacag
     2701 caggaacaaa gacggaaaac cattgcggag cgtcgctctg aaatggaaca ggtcaaacag
     2761 aaaaaacagc cagcttcttc cgttcatgaa agaccagcca ctaagcaaga acaatctcat
     2821 gatggacaga cttcaaggca aacaactgta caggcttcct atagggaatc tcaacaagcc
     2881 aaacaagagc gtccaacagt taagtccgat tcttcaagtt taaaaacgga acgtcaaagt
     2941 aatacagttc aagaaagaac ggttcaaaag ccagtaactt caactgcacc aacagataga
     3001 gcttcacaac gttcaatcac aaaagaacgt cagtctactg ttcaaagagt accactacaa
     3061 aatacaaaaa gtagaccacc aatcaaaacc gccaccatta agaaaggcag taagaaacca
     3121 tgaagctgaa aactttagtg attggtggtt cgggattatt cttgatggtc ttctcactgc
     3181 ttctgtttgt cgccatttta ttttcagatg aacaagatgg cggtttttcc aacattcact
     3241 atggcggtgt ggatgtttcc gcagaagtgc tggctcataa gcctatggta gaaaaagttg
     3301 ccaaagaata tggaattgaa gaatacgtca atatacttct tgcgattata caggtggaat
     3361 cgggtggtac agcagaagat gttatgcagt cctcggaatc cctcggtctt ccacctaatt
     3421 cattgagtac agaagaatcc attaagcaag gtgtgaagta tttcagtgag ttattagcca
     3481 gtagcgaaag gcttggtgta gatttagaat cggttatcca gtcctacaat tatggtggtg
     3541 gtttcttagg atatgtggct aatcgtggga ataaatatac ctttgaactg gctcaaagtt
     3601 tctcaaaaga gtattcgggt gacgaaaaag tgtcttaccc caatcccata gccataccta
     3661 tcaatggggg ctggcgatac aattacggga atatgtttta tgtgcaactg gtaacgcagt
     3721 atcttgtcac aacagagttt gatgatgata cagtacaagc catcatggac gaagctctga
     3781 aatttgaagg ctggaagtat gtctatggcg gagcttcccc gactacctct tttgattgta
     3841 gcggactgac acaatggacg tatggaaaag ctggaatcaa cttaccacga acagcacaac
     3901 agcaatatga tgtaacccgg catatcccat tattggaagc aaaagccggc gatttggttt
     3961 tctttcattc tacctataac gctggctctt atattactca tatcgggatt tatttaggcg
     4021 ataaccgtat gtttcatgca ggcgacccta tcggttatgc cgacttaaca agctcttact
     4081 ggcaacagca tttagtggga gcaggacgaa tcaaacaata agaaaggacg attcaatgat
     4141 gaaattcgga aaaaatcaga atacagaaaa acagacacca aaggaaaaga aacctcgtgt
     4201 ctataaggtc aatcctcgta aaaaggttgt gattgtccta tgggtacttt taggacttag
     4261 tttcagcttt gcgatattca agcactttac agccattgat acccatacca ttcatgaaac
     4321 caccatcata gaaaaggaat atgtcgatac tcatcatgtg gaaaattttg tagagaactt
     4381 tgcgaaagtc tactattcat gggaactaaa cgacaagtcc attgataatc gcatagaaaa
     4441 cctgaaagca tatctgacag aggaacttca agctctgaat gtcgatacag ttcgtaaaga
     4501 tattcctgta tcgtcttctg taaaaggatt tcagatatgg acggtagaag cagatggcga
     4561 caatcaattt gatgtaacct acagtgtaga ccaactcatt acagaggggg aaaatacaaa
     4621 gaccgtccac tctgcttata tagtgagtgt ctatgtagac agtactggaa atatggtcct
     4681 cattaagaat ccgaccatta ctagcatacc aaagaaatca gactataaac caaaagctat
     4741 tgaaagtgag ggtacggttg attccattac aaccaatgaa atcaatgagt ttttaacgac
     4801 gttcttcaag ctctatccta cagcgacagc cagtgaactt tcctactatg tgaatgacgg
     4861 gatattaaaa ccaatcggaa aagagtacat ctttcaagaa ctggtaaatc ctatttacaa
     4921 tcgtaaggat aatcaagtca cggtatcgct gacagtggag tatatcgacc agcagaccaa
     4981 agcaacgcag gtatctcaat ttgatttggt acttgaaaag aacgggagta attggaagat
     5041 tatagaataa caaatattgg tacattatta cagctatttt gtaatcacgt actctctttg
     5101 ataaaaaatt ggagattcct ttacaaatat gctcttacgt gctattattt aagtatctat
     5161 ttaaaaggag ttaataaata tgcggcaagg tattcttaaa taaactgtca atttgatagt
     5221 gggaacaaat aattggatgt ccttttttag gagggcttag ttttttgcac ccagtttaag
     5281 aataccttta tcatgtgatt ctaaagtatc cggagaatat ctgtatgctt tgtatgccta
     5341 tggttatgca taaaaatccc agtgataaga gtatttatca ctgggatttt tatgcccttt
     5401 tgggcttttg aatggaggaa aatcacatga aaattattaa tattggagtt ttagctcatg
     5461 ttgatgcggg aaaaactacc ttaacagaaa gcttattata taacagtgga gcgattacag
     5521 aattaggaag cgtggacaaa ggtacaacga ggacggataa tacgctttta gaacgtcaga
     5581 gaggaattac aattcagaca ggaataacct cttttcagtg ggaaaatacg aaggtgaaca
     5641 tcatagacac gccaggacat atggatttct tagcagaagt atatcgttca ttatcagttt
     5701 tagatggggc aattctactg atttctgcaa aagatggcgt acaagcacaa actcgtatat
     5761 tatttcatgc acttaggaaa atggggattc ccacaatctt ttttatcaat aagattgacc
     5821 aaaatggaat tgatttatca acggtttatc aggatattaa agagaaactt tctgccgaaa
     5881 ttgtaatcaa acagaaggta gaactgtatc ctaatatgtg tgtgacgaac tttaccgaat
     5941 ctgaacaatg ggatacggta atagagggaa acgatgacct tttagagaaa tatatgtccg
     6001 gtaaatcatt agaagcattg gaactcgaac aagaggaaag cataagattt cagaattgct
     6061 ccttgtaccc tgtttatcat ggaagcgcaa aaagcaacat agggattgag cagcttatag
     6121 aagtgataac gaataaattt tattcatcaa catacagaaa gaagtctgaa ctttgcggaa
     6181 atgtcttcaa aattgaatat tcggaagaaa gacaacgtct tgcatatgta cgcctttatg
     6241 gcggaatcct gcatttgcgg gattcggtta gaatatcgga aaaggaaaaa ataaaaatta
     6301 cagaaatgta tacttcaata aatggtgaat tatgtaaaat tgataaggct tattccgggg
     6361 aaattgttat tttgcaaaat gagtttttga agctaaatag tgttcttgga gatacaaagc
     6421 tattgccaca gagagagaga attgaaaatc cgctccctct gctgcaaaca actgttgaac
     6481 cgagcaaacc tcaacaaagg gaaatgttac ttgatgcact tttagaaatc tccgacagtg
     6541 acccgcttct acaatattat gtggattcta cgacacatga aattatactt tctttcttag
     6601 ggaaagtaca aatggaagtg attagtgcac tgttgcaaga aaagtatcat gtggagatag
     6661 aactaaaaga gcctacagtc atttatatgg agagaccgtt aaaaaatgca gaatatacca
     6721 ttcacatcga agtgccgcca aatcctttct gggcttccat tggtttatct gtatcaccgc
     6781 ttccgttggg aagtggaatg cagtatgaga gctcggtttc tcttggatac ttaaatcaat
     6841 catttcaaaa tgcagttatg gaagggatac gctatggttg cgaacaagga ttatatggtt
     6901 ggaatgtgac ggattgtaaa atctgtttta agtatggctt atatagccct gttagtaccc
     6961 cagcagattt tcggatgctt gctcctattg tattggaaca agtcttaaaa aaagctggaa
     7021 cagaattgtt agagccatat cttagtttta aaatttatgc gccacaggaa tatctttcac
     7081 gagcatacaa cgatgctcct aaatattgtg cgaacatcgt agacactcaa ttgaaaaata
     7141 atgaggtcat tcttagtgga gaaatccctg ctcggtgtat tcaagaatat cgtagtgatt
     7201 taactttctt tacaaatgga cgtagtgttt gtttaacaga gttaaaaggg taccatgtta
     7261 ctaccggtga acctgtttgc cagccccgtc gtccaaatag tcggatagat aaagtacgat
     7321 atatgttcaa taaaataact tagtgtattt tatgttgtta tataaatatg gtttcttgtt
     7381 aaataagatg aaatattttt taataaagat ttgaattaaa gtgtaaagga ggaattaatt
     7441 atgaagtgta atgaatgtaa cagggttaaa tacaaccaaa ttttaatttg gctttgcgtt
     7501 ttatcttttt ttagcgtatt aaatgaaatg gttttgaacg tctcattacc tgatattgca
     7561 aatgatttta ataaaccacc tgcgagtaca aactgggtga acacagcctt tatgttaacc
     7621 ttttccattg gaacagctgt atatggaaag ctatctgatc aattaggcat caaaaggtta
     7681 ctcctatttg gaattataat aaattgtttc gggtcggtaa ttgggtttgt tggccattct
     7741 ttcttttcct tacttattat ggctcgtttt attcaagggg ctggtgcagc tgcatttcca
     7801 gcactcgtaa tggttgtagt tgcgcgctat attccaaagg aaaatagggg taaagcattt
     7861 ggtcttattg gatcgatagt agccatggga gaaggagtcg gtccagcgat tggtggaatg
     7921 atagcccatt atattcattg gtcctatctt ctactcattc ctatgataac aattatcact
     7981 gttccgtttc ttatgaaatt attaaagaaa gaagtaagga taaaaggtca ttttgatatc
     8041 aaaggaatta tactaatgtc tgtaggcatt gtatttttta tgttgtttac aacatcatat
     8101 agcatttctt ttcttatcgt tagcgtgctg tcattcctga tatttgtaaa acatatcagg
     8161 aaagtaacag atccttttgt tgatcccgga ttagggaaaa atataccttt tatgattgga
     8221 gttctttgtg ggggaattat atttggaaca gtagcagggt ttgtctctat ggttccttat
     8281 atgatgaaag atgttcacca gctaagtact gccgaaatcg gaagtgtaat tattttccct
     8341 ggaacaatga gtgtcattat tttcggctac attggtggga tacttgttga tagaagaggt
     8401 cctttatacg tgttaaacat cggagttaca tttctttctg ttagcttttt aactgcttcc
     8461 tttcttttag aaacaacatc atggttcatg acaattataa tcgtatttgt tttaggtggg
     8521 ctttcgttca ccaaaacagt tatatcaaca attgtttcaa gtagcttgaa acagcaggaa
     8581 gctggtgctg gaatgagttt gcttaacttt accagctttt tatcagaggg aacaggtatt
     8641 gcaattgtag gtggtttatt atccataccc ttacttgatc aaaggttgtt acctatggaa
     8701 gttgatcagt caacttatct gtatagtaat ttgttattac ttttttcagg aatcattgtc
     8761 attagttggc tggttacctt gaatgtatat aaacattctc aaagggattt ctaaatcgtt
     8821 aagggatcaa ctttgggaga gagttcaaaa ttgatccttt ttttataaca ggaattcaaa
     8881 tctttttgtt ccattaaagg gcgcgattgc tgaataaaag atacgagaga cctctcttgt
     8941 atcttttt
//
LOCUS       DACKAC010000255         5771 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.guided.108, whole genome shotgun sequence.
ACCESSION   DACKAC010000255 DACKAC010000000
VERSION     DACKAC010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.guided.108"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            282..974
                     /locus_tag="I0B46_002953"
     CDS             282..974
                     /locus_tag="I0B46_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAR2085149.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
     gene            1301..1798
                     /gene="dfrG"
                     /locus_tag="I0B46_002954"
     CDS             1301..1798
                     /gene="dfrG"
                     /locus_tag="I0B46_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAR2085150.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(1870..3822)
                     /locus_tag="I0B46_002955"
     CDS             complement(1870..3822)
                     /locus_tag="I0B46_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAR2085151.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3829..4125)
                     /locus_tag="I0B46_002956"
     CDS             complement(3829..4125)
                     /locus_tag="I0B46_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2085152.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            4467..4757
                     /locus_tag="I0B46_002957"
     CDS             4467..4757
                     /locus_tag="I0B46_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR2085153.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
BASE COUNT         1962 a          917 c          998 g         1894 t
ORIGIN      
        1 gcgttttttg taataaatac agtaatctat gaaatgaaaa gggttacgtt taataaaact
       61 tgcttttttg tttttgttca ccataaatat ttatttaaga aagtattaac ctgcattttt
      121 ctctataaaa aagatttttt aaatagataa aaaaggagga ttaaatgagt atttgatagt
      181 atttatatta aaattacata tgtaatttaa aaacgcttga cattgtgttt gagatcactt
      241 acttttagca tgtaaaagta ttggattaag gagctatgca aatgaaaaag aaatttatta
      301 ctggtgggat tgtcgttgca tttctatgtc taattggcta cacaggcacg cggtacgcaa
      361 atgaaattat tttgtggggt ggagagacag atattgatac aatcaatgag aatctgacac
      421 agcttgatca tacattgaca gataaagagc aagcgattac agatcttacc gctcgactag
      481 cacaaaaaga acagacattg caagattcaa aggcagatgt ccaagaatac caaacaaaag
      541 taaatgaatt agaaaaccaa aaaaatcaat cggcagcaga aaaacaaaac ttagaaagtc
      601 aattagtcag caaggatgca caactacaaa gcaaacaaaa tgaaatcaat gtcaaactag
      661 atgagatcaa ccaaaaaaat gatgaaatca atcatctgaa tgcagattgg acagcaagac
      721 tacaagaagc acaagcaaaa ctttcagaat tacaaaatac tgtaaatggt ttgaatcaac
      781 aaatcgcaac actgacacaa gaaaaaacaa atgtagaaac tcagttaaca gatacacaaa
      841 agaaattaga cgaagcaact gctgaaaact caagcttgaa acaatatatt caaaagttag
      901 aaaaagcaaa agatgaagtg gaagatacag ctgataaatc tcagcaaatt gttgaagaac
      961 atttgcgtaa ataatctttc ttaacaagta aacctaaatt agtaaagaag caacagtaat
     1021 ctatactaag tagttcagtt ttggagtaca aaatgacaaa atgtacttca aaatggacta
     1081 cttttttcat tttaacttac ataccaaatt acatacaaaa tgatttataa aatgcaattg
     1141 aaaatgataa ttgaatgaaa atttgtgatt ttgtggtatt atgataatat aacttataga
     1201 aaaaattgct tattcaacta acgggcagga taatgagaaa aggttttttg atagaaataa
     1261 gaaattagat ggtaaattag ttaaaaaggc tgtgttataa atgaaagttt ctttgattgc
     1321 tgcgatggat aagaatagag tgattggcaa agagaatgac attccttgga ggattcccaa
     1381 ggactgggaa tatgttaaaa atactacaaa gggacatccg ataatattag gtaggaagaa
     1441 ccttgaatca atcggaagag ccttacctga cagaagaaat attattctga cgagagataa
     1501 ggggtttacc tttaatggtt gtgaaattgt tcattcaata gaagatgttt ttgagttatg
     1561 taaaaacgaa gaagaaattt ttattttcgg aggagaacag atttataatt tgtttttccc
     1621 ttatgttgag aaaatgtaca tcacaaaaat acatcatgaa ttcgaaggag atactttttt
     1681 tccagaagtg aattatgagg aatggaatga ggtatttgcc caaaaaggga taaagaatga
     1741 taaaaatccg tataactact attttcatgt atatgaaaga aaaaacttat tgagttaacg
     1801 ggtgcgttaa ttgaagaaga acagtaaaga taaagcacaa gagcttggga ttactccaag
     1861 ctctttttgt catttgaaat aaattatatc tttccaatca aattgtttat ctgtaatccc
     1921 taatctttga gcaggtgtca aaaccttttt atccgctgat ttaaaagggc gacaaaagtt
     1981 gtagtaagtt cttagaattg taatcgcaaa ttgagcatac ttcggattga agttagcgta
     2041 aatatagctc ttcttatcac cacgagctgt tgttaatggt ctttcaagag atgatatata
     2101 acgtcttatc tgttgaataa agctattggt cgaatggtca ttaacattta atagcatctt
     2161 tgcgatttcg tttggttcca gagcagataa atccgttcta caatcaactg aataaaatcc
     2221 tttatcctta gtggctaatg gatgtttgat agggttatcg gcatatttgc gatatctttc
     2281 tccatcttta ctaatagcct cttcgtggaa ttgatgactt gttagtaatt cagttagata
     2341 atgagatgcc aaagtccgta gaaactttgt tttgaatcct ctgatatctc cccaatcgag
     2401 cagttctgct tttgcttgtc cgaattcttt taagcattgt tttctgcttt tcgtcttgtt
     2461 aacttggcaa ataaaatgat gtgcatcgct caaccttaat tctttcgtga atactctata
     2521 aaaggcgttt ctaatagaga agtcatcatc actaatcatt cgccattcgt cggcattaac
     2581 catttgttta attaaccaat agtgagcagt tgtagtataa ggagcatcaa catttaaacc
     2641 atcgacaaat ctactcctat tagtaatttt ccctaattcg tgaagataag cattcctatt
     2701 ttcgctatcg ttagcggatg gttcttgtgg ataataagac caatctaacc tatcattttt
     2761 acgactaaaa gtattgagat ggtcttcttt gaattttctt gtatcttcat ttaattcatc
     2821 cattgaaata ttccaatcat aggcaacatc agaacggaac acatatcttg ataagacctc
     2881 agcagataca actatatacg tttgcatatt aaggtcttca aaccccgtat atttctttga
     2941 accttgtcct ttcttacgga cgttatttag gtagtagtgc atcttgtccg tattaagcca
     3001 catctcgtta aatttctttg tttgaagggg ttgtgtctca tatcgttcta aaaactccaa
     3061 acaacgccta tacagccact ccagcttatg atagtaggtt ccaactccaa ttcctaaaat
     3121 atcacaagta cgactgactg aaacccttcc aacaaccatc ttagcgaaca ttggaagaat
     3181 ggtgttcttt tgttggtgat aagtagtggt ttcttctctt tttggtagaa cattagtgaa
     3241 cttcttacac gccttacatt gatagcgttg agatttgccc ctacttttcc cccttttgta
     3301 aaattgcttc ggctcattga atggggttga ttcttcttcc gagcagcctt ctttatgaaa
     3361 ttgataatct ggctcaacat cttggatgct attgatttct ataaggcgtt taatttcttc
     3421 aacaacagac caattagata aaggggttac tgtacagttt tgcgatacac ctctgccaat
     3481 aggattgtca ttgcagtaca tacctttatg acctttatct ttggaactac cagtcatttt
     3541 atatcgactt ggtttccctt ttacatcttt gaatttgtgc tgttctttcc caaaattaca
     3601 acaaaacgga ttgatgcagt aattatattg aatgggatat tcagaaccgt tccatttaaa
     3661 agacgttggc aagaacaaca ttttgttata ccttgttgca aatcgttttg gagttaataa
     3721 atcatattgt ttagccctat cttttaattc ttcgtttgaa atcggaattt taacatctac
     3781 aatttcatct tccttcgtgg ctaatctttt aagcttggtc attgtctatc aacccactct
     3841 atctttgata cccatttttt caacaatccg cttattactt cgcttgttgg caatccattc
     3901 aataaacttc ttatcgtcta aaatgttcaa taagaattca tccactgctt cgctttcagt
     3961 acgctctgca tattcagcgt attgcttaac tatcaccctt acttgctctg atatttccca
     4021 atccacagac ttagcgttct tattttttgg ctcaagaaat tgcataggaa aatgcacccc
     4081 tttttgctta gtaaattgta accattttaa cacaccaatt tgcattccta caataattat
     4141 ttcacgttcg catttttata tacattttaa cttacatttt gtggtgtaaa ttaacaacaa
     4201 acgttgattt aacaataaaa aacgagtatc aaattcagtt gcaatttgat actcatttag
     4261 gtttacattt tcactttttg tactccaaaa ctgaactact taaatctata ctgctgcctc
     4321 ttttttttag tacatgtcct tcatcttcgc aggaagtgcc aatatgattt taagaaaggt
     4381 aatagacaaa aagtcaaaaa gataaaacag agtttcttct ttacaaaggg aatgtatgtt
     4441 cgtataatgg tattgaggtg aaggttatgg agaaaaaata tggaacagta gataagatcc
     4501 gtactttgca agttagtgcc agaccgttga tccgtttcag tttaaatggg gtcaattgtt
     4561 tgattgcttc ccatagtttg aacttcctag ctgaagtaga cgaagggatg aagttagtcg
     4621 ttacaggtta ttataataat agaaagcaat ttgttgtgag atcctatcat gtgttaggga
     4681 aacccaaaat tgtggtagaa tatgagaaaa gtctataccc aagaaaaaag gtccgacaag
     4741 atgaaagaat aaactagaaa acaagcggct gagacaaaaa tgtcctagcc gcttgttttg
     4801 gaaagatgga tgcagacgat gggatttgaa cccacacgag catacgctca caagaacctc
     4861 aatctatata taactacatg tatttaagta tttgtgatga atcactaaac tttccaaact
     4921 catttgaggg atattgctta aagattgaac agatcaattc gaagccctat tagtattcgc
     4981 tcaatggggg gatcagaggc cataaaaaga gactagtcat tactagtctc aggtagtgga
     5041 attgtaggat tttaaacgtt cctggagagt accggtatgt aactcgaaga ggtgattatc
     5101 atagtcgtaa aagtaaattg aagaagcctc gcctttcaca cgagaacgtc ctggttcgac
     5161 agttaaacct aacatttgga tttttgagac aaacgaatct atatcttgtt cgtcaatttt
     5221 aaaagcaata tgattgtatg tctttggcag tttattcgac gaattctgca ttactgcaat
     5281 ccataggtcc ttaactaaga aaaatttttc tggatataat gaaaattttt ttgttgacta
     5341 tcgtagattt cttttgcatc aaataaggta tttaacaatt aagtatttaa aagataactt
     5401 ttaagttatc tttaagtaat gcttaaagct atgttatact ctattaggaa atataagaag
     5461 gagtataaaa atgaacaaac ataatagaaa aataacgaat aacaaagaac tgatcttgtt
     5521 actaataggg atttggggta tctttttact ctttaatatg tatatgccga ctatgagagc
     5581 ggatgacgta gtttatgcaa gtagattaga tgaacttggc tatttgggtg cttcgataga
     5641 acattataag acatggagtt caagaattat tatagaacta ttcttaatgt tcttttctaa
     5701 acactttatg ctatggaaat tattaaattc tacgattatg ttaggaattg tagtattact
     5761 ttgcaagtat g
//
LOCUS       DACKAC010000256         5534 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.guided.111, whole genome shotgun sequence.
ACCESSION   DACKAC010000256 DACKAC010000000
VERSION     DACKAC010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.guided.111"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            93..557
                     /locus_tag="I0B46_002958"
     CDS             93..557
                     /locus_tag="I0B46_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAR2085154.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            541..1170
                     /locus_tag="I0B46_002959"
     CDS             541..1170
                     /locus_tag="I0B46_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAR2085155.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            1524..3002
                     /gene="msr"
                     /locus_tag="I0B46_002960"
     CDS             1524..3002
                     /gene="msr"
                     /locus_tag="I0B46_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAR2085156.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            3049..3495
                     /locus_tag="I0B46_002961"
     CDS             3049..3495
                     /locus_tag="I0B46_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAR2085157.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(3694..4209)
                     /locus_tag="I0B46_002962"
     CDS             complement(3694..4209)
                     /locus_tag="I0B46_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAR2085158.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            4326..5528
                     /locus_tag="I0B46_002963"
     CDS             4326..5528
                     /locus_tag="I0B46_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAR2085159.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         1721 a          927 c         1184 g         1702 t
ORIGIN      
        1 attacacagc tgattctttg acttttttct ttttttattc tattattgac aagtcaatcg
       61 ttgacgagtc aattattaga aaggaggaaa ttatgtcttt agaagagatt agtcaccttt
      121 tatatcaaat caaattagcc gatcaaaaaa tcgtccagat ctttgaagaa aatatcggta
      181 taagtcttac tagatacgag atcatgatga gactgaaaga agaaaatcca ttgctacaaa
      241 gcagtttgca agaaaagttg cagattgatc aaggagcagt tactcgtcat ctgaaggtat
      301 tggaagcaaa tggctatgtt tcgcgtcaga gaaatcctca gaacaaccgg gaagtgtatg
      361 tagcgctaac agaaaaagcc cagaagaaaa tcgagcattg tgaaatggac cataaagata
      421 caggttccgt gctatccccg gctttcagcg atgtagaaat gaaacaattt ctaacacttt
      481 tagataaatt gaatacccaa ctttgcaata taacaacaga aacgaaagag gttaataata
      541 atggaacaaa aaaatgattt ttcaacaatt atgactggta gaagatccgt tcgcgtatat
      601 gatgaaacat acaaaatccc acacgaagaa atgctggaaa tgatcaaaga agcgacgact
      661 gcaccttctt ctgtcaatat gcaaccatgg cgctttgtgg tcgctgaaag cgaggaagca
      721 aaagaaatct tgcgtccttt gatccgtttc aatacgagac aaaatgatac ttcttctgca
      781 atggtcatga tttttggcga tatgcaatgt tacgaatatg gcgaagaaat ctataaccaa
      841 gcttacgaat cagggaagat gccaaaagaa gtcagagatc aacaattagc tgcgatcatt
      901 ccttattaca agagtttttc aagagaagaa atgaacgatg tagtcaaagt cgattctagc
      961 ttagcggcta tgcaattcat gttagttgcg cgtgatcatg gctatgaaac aaatccaatt
     1021 ggtggattcg aagcagatca gttggcagag gcatttggtt tggataaaga ccgttatgtt
     1081 ccagttatta ttttatcaat aggaaaagcg gtagaagaag gttacgagtc tgttcgttta
     1141 gcaccagaaa agatcactac gttcgaataa aaggttttca gttactattc agtagctatt
     1201 gctttttttg aatcgtgtgt ggtaacctaa taaaaaagga ggtcggaaaa atgactgcat
     1261 cgatgaaatt acgtttcgaa cttttgaata acaactaaat aaattcaaaa gacatgtata
     1321 aacaacaatt tcatggatgg agtatgtcca ttttttcgaa cgacagtaga ataggtgaaa
     1381 gctatgacag atttggtgat ttagtcatag ttatttcgtt cagagtagga aaagatgtga
     1441 tgcagttttt ttgcaaacag atttttctgc tcttttttag tatcgataat tgttgtttta
     1501 catgaaatag gagtgtaaaa catatggaaa atttagcagt aaatataaca aatctacaag
     1561 tcagctttgg caaccagcta gaactatcta ttgattctct tcgtgtctat cagcaagatc
     1621 gtatagggat cataggagaa aatggtgtcg gtaaatcgac tttgctcaaa ctaatagccg
     1681 gtgaactttt tcccgatcat gggaaaatcc aaacagagat caccttcaac tacctgcctc
     1741 aattaaccta tctcgctgaa gcaaaggacc taaatttgga attagccagt catttccagt
     1801 taagactgga agaaacttca gagcggaaat ggagcggagg ggaagaacga aagatcgagt
     1861 tgatacgtct tctttcttct tatgaacaag ggatgcttct agacgagcca acaacccatc
     1921 tagatagaaa aagtattgat cgactgattg aagagcttcg ttattattat ggtacgctgg
     1981 tttttgttag tcatgatcgc tattttctag atgagctagc atcgaaaatc tgggaagtaa
     2041 aagacggaga aatccgagag ttttcgggga attatagtgc ctatctcact caaaaggaat
     2101 tggagaaaaa gactcagcta cgagaagcag agtcgatcat gaaagagaaa aaacgattgg
     2161 aaaaatcgat ccaagaaaag aaaaaacaag cggaaaagtt ggaaaaagtg tccagtaaaa
     2221 agaaaaagca acaaatcaga ccggatcggt tgtcttcctc taaacaaaaa gacagtgtac
     2281 aaaaagccat ccaaaagaat gcgaaaacat tagagagaag actccaaaaa ataggagaaa
     2341 caaccaagcc gcagcagatg aaacaaatcc gttttccagt accaaaatct cttgaactcc
     2401 acagccgcta tcccatcatg ggacaaaatg tccaattgga aagaagtggg agaacattac
     2461 tggtaaatgg tgattttcag ttttctttag gtaaaaaaat cgcgattgtc ggtgaaaatg
     2521 gttcaggtaa gacaacctta ttagaacata tccgcaaaca aggagaagga atccttctct
     2581 ctccgaaagt aagctttcaa gtatatcagc aaaagggtta tcaaatgaca tctgaagaat
     2641 ccatcattcg ttttgtcatg agacaaacag agttttcaga atcgcttgtc cgtagtttgc
     2701 tgaatcactt agggtttgct caggaaactt tgacgaaacc gttatgtaca ttaagtgggg
     2761 gagaagcgac ccgtctgacg attgctttgc tttttactaa gccaagtaat gtgttgctgt
     2821 tagatgaacc gactaatttt attgatatgg caacgatcga agctttagag aagctaatgc
     2881 aaatatatcc gggaacgatt ttgtttactt ctcatgattc ttactttgtc gagcgtacgg
     2941 ctgatgaagt ttatgaaata aaagggcaga aaataaaaaa agtacttacg agaaattttt
     3001 aaatgtgcta cgatgatttt cgtgacaaat ttaaaggagt ggtacagatt gcctacaaat
     3061 aaaaaagaag gaataatttt tacaacaatg atgtgttttt tgatggtctt ggggatgagc
     3121 gcgtataatt tatggctgca tcaagatctt catttatcaa gtttgttgat tggtctagtg
     3181 cctggattcg tggtggcttt cattttggat gtcttcatca tcggtgtgct ggcgaaaaaa
     3241 attgccttta aactaccagt aaatccagaa agcaaggtcc aaatgatctt aactatttca
     3301 tgcttgatgg ttttaggaat ggtgacatgt atgtctttat tcggtgtgat catggaacaa
     3361 ggaatacctg atcaattatt ctctgcttat atgaatgctt ggaaaatgaa tgtgattgcc
     3421 gctttaccgt tgcagttgat catcgttggc ccgctttcaa gaagtgtgct aaaagcaatt
     3481 caaagtaatg aatgatggaa cactgttatt tgaaaaaagg ccttggcatg ctttttgtca
     3541 tagtctcttg aatgcagttc tagaaaaata gtgatccgaa cgataggttt tggcctgtcg
     3601 ttcggatcgc tttttctttt ccaactgtat tgctctttta ttgaagataa tttgctcttc
     3661 aatagatatt tctagaaagg ttctgtttat cccttactca aaaaactgct gcatttgttc
     3721 tcttacttgc tgacaccatt ggagttcctg ctgacaaagc ataattctgc gctggatcac
     3781 aacataacgt ccaaattctt gtttcttgtc tagcgagagg tcattcaatt ttttttgatt
     3841 tttttgcatg attttgttca gctgttgttc tctagtgaat agcagtccag tagctgttgt
     3901 tttatcgagc gtcgaaaaag catagatttt tgctagaaat tcatcttttt gcgtaggagc
     3961 gggagtttcc tctttgatcc attcctctac aagagcacgt ccttcatcag tcaagtggta
     4021 gatctttttt tgagagtgct cgtctttgtc caaaacggtc acataaccct gctggtctaa
     4081 cttggcaagc gttgtataga tctgactgtg atgggcttcc caaaaaattg tgatcagctg
     4141 cttcaattca tacccagaac gtggtttgga taaaagcacg cataataata tataggctaa
     4201 agtattcata aaaatcctac ctctcgtctt attttcttta ttatatcaca gtaaagtttc
     4261 cacgttttac atgtgcatat ttacatgtaa aataaatcat gtatgatgct aatagagaga
     4321 aagggatgga aatattggag aatcaaatag atatacaaga acaaacaaat gaagaaacaa
     4381 aggggaaaag ttcagcgcta gttgtagcag ctacctgtat gatcgctttt atgggagtgg
     4441 gtctagtaga cccaatctta aaaacgattg ctgcacagtt aaacgctacg ccagctgaaa
     4501 cgaccctgtt atttactagt tacatgttag tcactggtgt ggtgatgcta tttacaggtt
     4561 tcttatctag tcggatcggt ttgaaaaaaa cattgatgac aggattgttt atcatcgttg
     4621 tattcgctgg tcttggcgga ttatctggga atatcggaat gctgattggg ttacgtgccg
     4681 gctggggtgt cgggaatgct ttatttgtct ctactgcttt agcaacgatc gtcagtattt
     4741 tagttggtaa cacagaaaaa gcaatcatga tgtatgaagc agcacttggc tgcggaatgg
     4801 ctgttggccc tctagtcggt ggggtcttgg gcagtggttc atggcgttat ccattttttg
     4861 gggtagcatt tttgatgttt tgcggtttta tagccctagc ggtactttta cctgaaactg
     4921 agaaaccaaa gagaaaagta ggcctttggg aaggtgtcaa agcgttaagc aatcataaac
     4981 ttcgttcagt tggactaatc gcattgttat ataatttcgg attctttact ttacttgcgt
     5041 atgcgccatt tttattgatt ggctattctg aattagaagt cggttttgta ttttttggct
     5101 ggggtgtgct tctggctatt gcttcgattt tcgttgcacc ggctcttgag cggaagttta
     5161 cgacttatcg gacaatttta gcagcatttg tcttattcat tatttgtttg ctgttattag
     5221 gagctggaac ttctatgcca gtccttgtac cgatcagcat tgtgttagca ggatttttcc
     5281 aaggaattat caatacactg ttgacgacga ttgctatgga aataccaggt ctgcaacgaa
     5341 acgttgcttc gtccagctat agttttgtcc gctttttcgg tggagcttta gcaccattta
     5401 ttgcaggtgt gattggtgaa catattggtg aaagctatgc gttttatttt gcaggtgcca
     5461 ttgttcttat cagtgtatgt tgtatcattt atcaccgtaa ttattttgct acaaatgaag
     5521 aagaataaga aatc
//
LOCUS       DACKAC010000257         4036 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.guided.125, whole genome shotgun sequence.
ACCESSION   DACKAC010000257 DACKAC010000000
VERSION     DACKAC010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.guided.125"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..279
                     /locus_tag="I0B46_002964"
     CDS             <1..279
                     /locus_tag="I0B46_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAR2085160.1"
                     /translation="KMMPTDEVETTGAPEQPQDELFEEAKALVVEMQTASISLLQRRF
                     RIGYNRAARLVDELEAHGVVGPSEGSKPRKVFIEQEETSETLPDDNDM"
     gene            509..1576
                     /gene="rlmN"
                     /locus_tag="I0B46_002965"
     CDS             509..1576
                     /gene="rlmN"
                     /locus_tag="I0B46_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAR2085161.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(1769..2872)
                     /locus_tag="I0B46_002966"
     CDS             complement(1769..2872)
                     /locus_tag="I0B46_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAR2085162.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            3425..3973
                     /gene="aac(6')"
                     /locus_tag="I0B46_002967"
     CDS             3425..3973
                     /gene="aac(6')"
                     /locus_tag="I0B46_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAR2085163.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         1337 a          687 c          827 g         1185 t
ORIGIN      
        1 aaaatgatgc cgacagatga agttgaaacg acaggtgctc cagaacagcc acaagatgag
       61 ctgtttgaag aagcaaaagc acttgtcgtt gaaatgcaaa cggcaagtat ttcccttctc
      121 caaagaagat ttcggatcgg ttacaaccga gcagcacgtt tagtagatga actggaagct
      181 catggagttg ttggcccgtc agaaggaagc aagcctcgga aagtattcat tgagcaggaa
      241 gaaacatcag aaacgttgcc ggacgataat gacatgtaaa acttgtgtga tgaagggttg
      301 aaaaacgaat aaccaataaa aagacaaggc atgatgatac caaaagaaaa aatggttttt
      361 ttccatcttt tctaatacgg tatgatgtct tgtcttttta gtttttgtga gattaaagtt
      421 ctattcgatt tatttgtgaa gtattgcatt aaggaaatga atcatgtata gtatgtagcg
      481 aaactttgag aaaaagaggg aaaatacttt ggaaaaacag tccatttatg gcttgacgaa
      541 tgaagaatta atcaattggt ttatagaaaa cggcgagaaa aaattccgag ctgcccaagt
      601 ttgggaatgg ttgtatcaaa aacgtgtaag taatttcaca gaaatgacga atctatcgaa
      661 acaattgatc gaaaaattat cggagcattt tatcatcaat cctctaaaac agatggtcgt
      721 ccaagaagct tcggatggta cagtaaaata tttgtttgaa ctccctgata aaaacatgat
      781 tgagactgta ctgatgagac aagaatatgg cttgtcagtt tgtgtgacga cacaagtagg
      841 atgtaatatc ggctgtacgt tttgtgcaag cgggctgctc aaaaagaacc gtgatctgac
      901 tgctggagaa attgttgcac agatcatgat ggtccaacat tattttgatg agcgcaatct
      961 tggcgaacgc gtttcacatg tcgttgtaat ggggatcggt gagccatttg ataattacga
     1021 caatgtaatg gactttttgc atatcatcaa tgatgccaaa ggattggcaa ttggtgctcg
     1081 gcacattaca gtttccacta gtggattagc acacaaaatc aaggaattcg ctaataacgg
     1141 cttgcaagtc aatctggcta tttccttgca cgcgccgaat aatgaggtgc gaacatcgat
     1201 catgcggatc aaccgaagtt tcccaattga gaaattgatg gaagctgtgg atgagtattt
     1261 agagaaaaca aaccgtcgaa tcacgtttga atatatcatg ttgaatcaag taaatgatag
     1321 accagagcat gcgcaacaat tagcagatct attaaaagat aagaaaaaat tagcatatgt
     1381 caacttgatt ccatataatc cagtcagtga acatgatcaa tattctcgca gtccaaaagc
     1441 tgatgtattg agattttatg atgtattgaa gaaaaacggc gtaaactgtg tgatacgtaa
     1501 agaacatggt acagatattg atgcagcttg cggacaattg cgaagcaagc aaatgaaaaa
     1561 aacagttgcc aaataatcgt atcctactcg ttttttcaat taccataaaa ggagcagttt
     1621 tcttaattaa ggaactgttc aacttaaata agagaaaaga ttctatagcg aaagctataa
     1681 agcttttttt gacaagaaaa accctcatca agcaaagaaa tgtcctcaat ttgatgacag
     1741 cctgcttgat gggggtttta gcaattattt aactgatttt cttatatcct tgctggcttt
     1801 cttgtgtagc atgataagtt tctacgtcaa gaatggcctg tttaaatttt tctcgggtga
     1861 tttcataagg cacatgttta atatcatttg ttgccatggc tttatctaag attgcttcta
     1921 aatcggtcgt gacattgaga ttcatgtcgg ttaacgatac tggaagttgg ttctttttca
     1981 taaacacata gattttatca aacaaatcga actgctgatc catcatcaat aaggtcagta
     2041 caccataact gacagatgct ccgtgaaggt atccctccat tttttctaga acagtcgtac
     2101 cgtaatagaa tgagtgtgcc atagcagaat tatagttatt gttaactaaa acagaaacca
     2161 atccggtagt aacaatgata tccgaaacga cttgttcaat tgcatatgag ggacgatcgt
     2221 cggcactatc tttcaatgct tgcgtaccat aagtcaagag acgatcaatg cacatttttc
     2281 cgatttggat tcctaatcta tccgtgtagc ctagatcatc gccacgagca gaaaaagtag
     2341 attcgacttc tttggataaa gcatcgccaa tacctgccca taagtattta tcaggtgctt
     2401 cggcaataat tgctgtatca atgaaggtgt gtacaggtgg ttttgaagga aagaagagtc
     2461 cgtccattac gccttcttcg ttatagatga cacataccgc agtgacgggc gaacagttcg
     2521 aaccaatagt gggaaaggtg aagactggtt tagctagttt ttcaccaatg acttttacag
     2581 tatcgcaagc acggccacca ccgactgcaa acaggacgtc tgctttttgg acactttcat
     2641 gctggatcaa ctgttcaata tttccataag ttgaattacc cccgtaccag agactggcga
     2701 caacctctat ttcctgtgtc aaagctactt ttattttgtt ttctgccttt gaaagtgcag
     2761 tcttccctcc aatcagcact acttttgtcc catattcttt gacgattcca tttgcttttt
     2821 catacgcatc tgttccaatg gtataatttg gtaagaaaac actttgactc atattcattt
     2881 ccttctttct tcgttgaaac gggttaaaat tttaaagagt gatcgatctg actgcggctg
     2941 tagatggagt agaggtgaac ctaagacagg ataagtgatg aataaggtgg catgaacacc
     3001 ttgaaatcat caataagtat cctaaaaacg aacaagtaca aaaacattga aaacgacaat
     3061 aatgaatttt tgttcctctt agtatttcta ccgattccct ttgctttttt gtaattatct
     3121 aaaaattcta aaaattaaga ttattatatg aaaacaggtt ttatttgtca attcataatt
     3181 ggtttttttt aaagcaaaag ataatctata tcatgatgaa caaacagagt tttttcctcc
     3241 aattgatggc tttcctgatc tttcctattt tgaataaagt gtttctgtat aattttttta
     3301 ttttataagt aagataaata atgaaagaat agagatactg attggtagct aaagattttt
     3361 gagcgagaac atttcgtttt ttcaaaagga ctgttagaat aagcaagaac aagggggaag
     3421 acgtatgata atcagtgaat ttgaccgtaa taatccagta ttgaaagatc agctttctga
     3481 tttactgaga ctgacttggc cggaagaata tggagacagc tcggcagaag aagtagaaga
     3541 aatgatgaat ccagaacgaa tcgcggtagc agcggtagac caagatgagt tagtaggatt
     3601 tattggtgca atccctcaat acggtatcac aggttgggaa ttgcatccat tagttgtaga
     3661 aagctcccga cgaaagaacc aaataggtac tcgattagtc aattacttag aaaaagaagt
     3721 agcttccaga ggaggaatca cgatttattt aggtacggat gatttagacc atggaacaac
     3781 gttaagtcaa acggacctgt atgagcatac atttgataaa gtggcttcca tccagaacct
     3841 tcgtgaacat ccgtatgaat tctatgaaaa attaggttat aaaatcgtag gtgtcttacc
     3901 aaatgcaaat ggctgggaca aaccggatat ttggatggca aaaacgatta ttcctcgacc
     3961 aaattctcaa taataaaaag aggttgtgac agaagtaatc agcttcaaga aataagaagg
     4021 aatttacgaa aattac
//
LOCUS       DACKAC010000258         1390 bp    DNA     linear   BCT 16-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VRE32604
            SAMN09401217-rid8189313.guided.170, whole genome shotgun sequence.
ACCESSION   DACKAC010000258 DACKAC010000000
VERSION     DACKAC010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09401217
            Sequence Read Archive: SRR7297046
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581748.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581748.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/02/2020 00:16:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,829
            CDSs (with protein)               :: 2,829
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 39 of 74
            Pseudo Genes (incomplete)         :: 27 of 74
            Pseudo Genes (internal stop)      :: 23 of 74
            Pseudo Genes (multiple problems)  :: 13 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09401217-rid8189313.guided.170"
                     /strain="VRE32604"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..1311
                     /locus_tag="I0B46_002968"
     CDS             <1..1311
                     /locus_tag="I0B46_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HAR2085164.1"
                     /translation="ESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYH
                     YPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAI
                     RAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVD
                     SIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIE
                     YSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISE
                     CTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSC
                     NHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIE
                     KAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          597 a          103 c          235 g          455 t
ORIGIN      
        1 gaatcattaa aaaaacatta tacagagcct tgggaagatg aagtttttag agtaattatt
       61 gaatataaca atgttcctat tggatatgga caaatatata aaatgtatga tgagttatat
      121 actgattatc attatccaaa aactgatgag atagtctatg gtatggatca atttatagga
      181 gagccaaatt attggagtaa aggaattggt acaagatata ttaaattgat ttttgaattt
      241 ttgaaaaaag aaagaaatgc taatgcagtt attttagacc ctcataaaaa taatccaaga
      301 gcaataaggg cataccaaaa atctggtttt agaattattg aagatttgcc agaacatgaa
      361 ttacacgagg gcaaaaaaga agattgttat ttaatggaat atagatatga tgataatgcc
      421 acaaatgtta aggcaatgaa atatttaatt gagcattact ttgataattt caaagtagat
      481 agtattgaaa taatcggtag tggttatgat agtgtggcat atttagttaa taatgaatac
      541 atttttaaaa caaaatttag tactaataag aaaaaaggtt atgcaaaaga aaaagcaata
      601 tataattttt taaatacaaa tttagaaact aatgtaaaaa ttcctaatat tgaatattcg
      661 tatattagtg atgaattatc tatactaggt tataaagaaa ttaaaggaac ttttttaaca
      721 ccagaaattt attctactat gtcagaagaa gaacaaaatt tgttaaaacg agatattgcc
      781 agttttttaa gacaaatgca cggtttagat tatacagata ttagtgaatg tactattgat
      841 aataaacaaa atgtattaga agagtatata ttgttgcgtg aaactattta taatgattta
      901 actgatatag aaaaagatta tatagaaagt tttatggaaa gactaaatgc aacaacagtt
      961 tttgagggta aaaagtgttt atgccataat gattttagtt gtaatcatct attgttagat
     1021 ggcaataata gattaactgg aataattgat tttggagatt ctggaattat agatgaatat
     1081 tgtgatttta tatacttact tgaagatagt gaagaagaaa taggaacaaa ttttggagaa
     1141 gatatattaa gaatgtatgg aaatatagat attgagaaag caaaagaata tcaagatata
     1201 gttgaagaat attatcctat tgaaactatt gtttatggaa ttaaaaatat taaacaggaa
     1261 tttatcgaaa atggtagaaa agaaatttat aaaaggactt ataaagattg attatataat
     1321 atatgaaaag ctattataaa agacattagt attaaatagt ttaaaaaaat gaaaaataat
     1381 aaaggaagtg
//
